LOCUS       DAOSNB010000001        44312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_901_length_44312_cov_4.543485, whole genome shotgun sequence.
ACCESSION   DAOSNB010000001 DAOSNB010000000
VERSION     DAOSNB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 44312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44312
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_901_length_44312_cov_4.543485"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..2405
                     /locus_tag="PLG42_00005"
     CDS             312..2405
                     /locus_tag="PLG42_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU29620.1"
                     /translation="MRLTGYSLLLILLILTCRSANAQTNVEIEKSQFRNVRDGFKVAW
                     EHVKEGDAYFMEGGVLYRNAFEEYKKAYAYNSTNAELNYKIGVAALFSDNQDKAAEYL
                     LKAYEINNKVAEDVLLLAGRALQYAGRYNEAIEKLELYIDSSNKRNKAGVEMAKKYIE
                     ECKSAIELTKDTLRIEIQNLGGSVNSDCDDYSIVPTGDGKRIYFGSRRATSGSSSKRY
                     DDKKFDENIFITDFQNGVWTNALPVGSKINTEYCETPLFLSKAEDRLFVYAGYEGNGD
                     IMVSELRRGSWRAPVKEKIGISSNFPETSMCISPDGNEVAFIRDAGKKGIGGKDIYIV
                     KRIQDKRWSKPQNAGITVNSRYDEESVRYSKGGDTLWFSSAGHNAIGGFDIFYSVRST
                     DGSWGPAMNAGFPINTPWHELFYVPAPDDDSAFYFVSNRKGGYGGLDIYKGRLLPPPP
                     VVTPPEPEPEPVKPPEPQIVVVRDTVVIIKEVAPPAPVIDKSVYLTGNITDSETGAPV
                     LARIEVIDYLTDAVIATTASSDADGSYKVKLPERKIYIVNVRATGFLSDMKKISIPED
                     YPSDLMRLDVQLAKVKVGKKVVLNNIFFETGKAVLTKDSFEELDKLVGIMNDNPSMKI
                     EISGHTDNTGSAVINARLSTERARAVVEYLVSKGIDRARMTYRGYGPDQPIADNSTPE
                     GRAKNRRVEFKILEM"
     gene            2494..3600
                     /gene="gcvT"
                     /locus_tag="PLG42_00010"
     CDS             2494..3600
                     /gene="gcvT"
                     /locus_tag="PLG42_00010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOU29621.1"
                     /translation="MKHTPFTQIHIGLGAKMVPFAGFYMPVEYTGIIDEHITVREKVG
                     VFDVSHMGEFRVKGPNAFAFLQYITSNDVSALYDGKVQYSCFPNGRGGIVDDLLVYRF
                     SSDDYLLVVNAANIEKDWQWCVQHARRFGILPGKEIINLSDSTAQLAVQGPLALKAMQ
                     KLTSASIEGMEYYTFKVIEFAGIKDVIFSTTGYTGAGGCEIYVKNEDGPRLWEAVFEA
                     GKEFGIKPAGLGARDTLRLEMGYCLYGNDIDDTTSPIEAGLGWITKFVDGKDFIDREL
                     LLKQKTEGTSRKLRGFEMIEKGIPRQHYEVFDSAGNKIGEVTSGTMSPMLKKGIGMAY
                     LTRGFWKPGTEIFIRIRNKDLKAVIVELPFYKKQ"
     gene            3691..4416
                     /locus_tag="PLG42_00015"
     CDS             3691..4416
                     /locus_tag="PLG42_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOU29622.1"
                     /translation="MEKLKLEVCLSPAMFHRYDCRGNIVVIIDVLRACSAICTAFENG
                     AAEIIPVAEAEEAKEYKNRGYLVAAERDGFTLDFADFGNSPFNFTRERVEGKTIAYST
                     TNGTRIIEMTDKSYKTIIGAFLNISSVTEWILAEKKDVILFCAGWKDRFSLEDTVCAG
                     AYASRLLESGLFTTICDSVHASLDIWEIARNDLRGYIEKAAQRSRLRDKGLDDCIDFC
                     LTADITPVIPVVEKGKIVPLRYK"
     gene            4530..5600
                     /gene="serC"
                     /locus_tag="PLG42_00020"
     CDS             4530..5600
                     /gene="serC"
                     /locus_tag="PLG42_00020"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOU29623.1"
                     /translation="MKKHNFYAGPSILPQFTIEKTIEGIKNFAGTGLSVLEISHRSKE
                     FIACMNDTIALVKELLDVPQGYHVIFLGGGASLQFCMVPINLLKNKAAYLVTGEWASK
                     AFKEAKLIGEAVEVASSKDRNFSYIPKNYTIPADADYFHITTNNTIYGTELKEDPDSP
                     VPVVADMSSDIFSRPVDVSKYAIIYAGAQKNLGPAGVTMVIVREDVLGKVERTIPTML
                     DYRTHIKAESMFNTPPVFAVFAAQQTLLWLKEMGGVRVMQKRNEEKAKILYDEIERNK
                     LFMPTVSDPADRSIMNVCFVMKPEYKELEVEFLDFAKSRGMVGIAGHRSVGGFRASLY
                     NALPKESVEALVEVMKEFESKK"
     gene            5605..6522
                     /locus_tag="PLG42_00025"
     CDS             5605..6522
                     /locus_tag="PLG42_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOU29624.1"
                     /translation="MKILLATEKPFSPAAVRKMRELVEAEGHQFVTLESYTSIEELLH
                     AVADASALIVRSDPVTAQVLDAAPELKLVVRAGAGYDNIDIKACTARNVIAMNTPGQN
                     SNAVAELAFLMMLYMARSGFAGKWGTELRGKSIGLHGFGNVSRFMNQIAKGFGMKVYA
                     YDPYVEENEMKQSGVIPCKTPEELYSNCQYVSLHIPLNNKTRKSIGYELLKLMPPEAV
                     LVNTARKEIIDEDGLLKIFDERSDIKYLSDIEPDCKAIFEQKYSGRFLFTAKKMGAQT
                     EEANTNAGIAAVKQIIDYFKTGNDKFRVN"
     gene            6544..7794
                     /locus_tag="PLG42_00030"
     CDS             6544..7794
                     /locus_tag="PLG42_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOU29625.1"
                     /translation="MAVVKPFRGLRPPASIVKDLACLPYDVMNTEEARQMAKGKECSL
                     LHITRAEIDLPEGTDIHSREVYDKSVENFRLWRERGWLVQDKEPHFYIYAQTMGGRTQ
                     YGIAGCASVDDYYNGIIKKHELTRPDKERDRMIHIKVNNANIEPVFLAYPAVDEIDKI
                     VTEITQKQKPEYDFISEDGIGHHFWVIWDKDTNRKIEELFRTRVPYTYIADGHHRTAA
                     AALAGKEKRESNPLHRGDEEYNFFLAVHFPDNQLKILDYNRVVKDLNGLTPKELIERI
                     KASFSVEDKGTEIYHPRHLHNFSMYLDGRWYSLTAKQGTYNDDDPVGVLDVNILTNHI
                     LAPVLGIEDLRRSTRIDFVGGIRGLEELKKRVDSGEMQVAFALYPVSIKQLINIADTG
                     NIMPPKTTWFEPKLRSGLVIHLLD"
     gene            7807..8487
                     /locus_tag="PLG42_00035"
     CDS             7807..8487
                     /locus_tag="PLG42_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOU29626.1"
                     /translation="MDIALNIKRLRDEVPEHVKIVAISKTRPASDIMIAYQAGHKIFG
                     ENRAQELLSKKDLLPQDIEWHFVGHLQTNKVKMVVPNVKLIHSVDSFKLLNAINKEAA
                     KLNLSVECLLQFHIAREETKSGFTFEEALRMLNSQEIKEMNNVIIRGVMGMATFTDDM
                     KVVRNEFRTLRKYFNILKDRYFIDNKYFTEISMGMSGDYRIAIEEGSTIIRVGTLIFG
                     ERNYFEND"
     gene            8497..9474
                     /locus_tag="PLG42_00040"
     CDS             8497..9474
                     /locus_tag="PLG42_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOU29627.1"
                     /translation="MSKLSTKYLGLELKSPVIAGSSGQTSSLEKIKEAENCGAGAIVL
                     KSLFEEQIEHLIASSGGPHSYPEAEDYMAYYIKANAVENYLNLISAAKKSISIPIIPS
                     INCISAGSWIDYAKMIENAGADALEINIFFLPVKGETTGSEIEKSYLQIIEKLKSKVK
                     VPIAVKIGFRFTNILNIIELLYKRGVEGVVMFNRFYEPDIDIDKMAVVPAEVFSLPAE
                     RRYVLRWIAMADALDFKIDISASTGIHSGDDAIKYLLAGADTVQVCSALYKKGIGFIK
                     EVNERIISWMEKRGFNSINQFRGKLNYSAVENPILYERAQFMKFFSSHD"
     gene            9768..10676
                     /locus_tag="PLG42_00045"
     CDS             9768..10676
                     /locus_tag="PLG42_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29628.1"
                     /translation="MNKLIRTGIITVIILSLFVLPGCKSGGEKQKKIEPVTQKDVQKI
                     KEDISKAEKMFNALPSPLESAMLIKTAGASFDRRYLNPIENVKLYTTTRSQALNLGIY
                     TCDLSFASLYDQTQLIIDYMNAAKKMADGLGILDAINQETISRLEENINNKEVIMEII
                     SETFMNSNSYLADNDQAAVAAIVLTGGWIEGLYIATQLVDLNNFNGNKLVATIIDQKL
                     SIDILINLLESNKGNPAVDELISQVNKLKAVFDKINIQTTPIKTEIDPATNVTILKSK
                     VTTDMSVETFKELTAVVKEIRDSFVK"
     gene            10684..11127
                     /locus_tag="PLG42_00050"
     CDS             10684..11127
                     /locus_tag="PLG42_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29629.1"
                     /translation="MKTVRLFSGILLLLIALSIPAHSQCKAFAKKEVLPELSPYVHDG
                     NYHAAILVEGEEAELYKTFYSDMEYRVVVMGESKLPAVEFKVLDENKNVLYSSKDNGN
                     SSSWDFKLESSKQLKLVIKVSSFNKPGDVPASGCVAIMFGFKVKK"
     gene            complement(11185..13977)
                     /gene="uvrA"
                     /locus_tag="PLG42_00055"
     CDS             complement(11185..13977)
                     /gene="uvrA"
                     /locus_tag="PLG42_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU29630.1"
                     /translation="MEGNIEIRGARVHNLKNISLSIPRNKLVVITGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGRMQKPEVDFIDGIPPAIAIEQKVNTRNPRSTVGTS
                     TEIYDYLRLLYARIGRTYSPVTGREVRKHSVDDIVDFLKTLPENTTVIITSPAEIPSK
                     ITTKEFFLFLVKQGYNRIEKDGDLIRIDDPDSPLCCNPGEKINIVIDRLLVSHSSEGI
                     SRAADSIQTALSTGKGFCQLVIYEGGRITRENFSDKFEENGIQFIEPSEHLFSFNNPI
                     GACPVCEGYGKIIGIDENLVIPDQNLSVYDDAIVCWKGESMKKWKERLISTAHLFGFP
                     LHKPYYQLTPEQKEVLWKGNRYFWGINQFFEYLEEKKYKIQYRVLLSRYRGKTTCPAC
                     KGTRLRKEASYVKIGGKSIQELVSMPVDELLNFLNTLKLTQQEQKIAERLLHEITSRL
                     KFLLNVGLPYLTLDRLSSTLSGGESQRINLATSLGSNLTGSLYILDEPSIGLHPRDIN
                     LLTSVLKELRDMGNSVLVVEHEEEIIRAADEIIDMGPGAGLFGGEVIFQGKIDSTQSN
                     NSLTIKYLEGELEIPVPPVRRPWNSYIEVKGARQNNLKSIDVKFPLHTITVVTGVSGS
                     GKSSLVHDILYRALAERIEGSGIRPGNYREISGDINSLTGVEMVDQNPIGKSSRSNPV
                     TYLKAYDEIRKLFAEQQASIQNNFKASHFSFNIDGGRCEDCLGEGIIKVEMQFMADVE
                     LVCETCKGMRFKKEILEIKYHGKNIYDILEMTIDEAISFFSSYNGKTERKIVEKLKPL
                     VDVGLGYVKLGQSSSTLSGGESQRVKLAYFLSQEKAGGHILFIFDEPTTGLHFHDISK
                     LLGSLNALVEHGHSVILIEHNPEVIKCADWVIDLGPEGGDKGGYIVFEGKPEDLIKCK
                     KSYTGKFLSNKLTKTK"
     gene            14147..14734
                     /locus_tag="PLG42_00060"
     CDS             14147..14734
                     /locus_tag="PLG42_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU29631.1"
                     /translation="MKKETSDYELLKRFIDGEQSCFEELIHRHKNKVYAYISLYIRDQ
                     ALAEDIFQDTFLKVIQSVKAGKYYEDGKFLSWVMRIAHNLIIDHFRRIRQMNTMSNDD
                     YESDLFNSKSLAEKNVEEDIVRIQVRKDVRKLISMLPYDQREVVILRHYTGLSFKEIA
                     EITGVSINTALGRMRYALINMRKMINEKKISLTVK"
     gene            14786..14974
                     /locus_tag="PLG42_00065"
     CDS             14786..14974
                     /locus_tag="PLG42_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29632.1"
                     /translation="MNLNSTPFVKYDEIDVEDADLIDEAWDFRAEFHDVFELLDTLKA
                     EPDRQVTERLMEIIEGQM"
     gene            15083..15937
                     /locus_tag="PLG42_00070"
     CDS             15083..15937
                     /locus_tag="PLG42_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU29633.1"
                     /translation="MKKEFVIKPMSGYIALLAALLFLGGAIAGFIYELIWPGIVLTVL
                     FIFTVIGLTIVYPNESCVTVLFGAYRGTIKENGFFWVNPFYLRRKISLRARNFDSEPI
                     KVNDKIGNPIKIGLVLVWKVEDTYKAAFEVDDYQHFVLVQSDAALRKLAGLYPYDNFE
                     DHEATLALRSGGEEINNELEREISERLAIAGINVIEARINYIAYAEEIANAMLRRQQA
                     TAVVSARYKIVEGAVSMVQMALEQLSDKKIVELDEEKKAAMVSNLMVVLCADRDATPV
                     VNTGTLYT"
     gene            15941..16135
                     /locus_tag="PLG42_00075"
     CDS             15941..16135
                     /locus_tag="PLG42_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOU29634.1"
                     /translation="MVKKKAFILRIDPEKLEALEKWAADEFRSTNGQIEYIIDQALRK
                     AGRLKSKPVSETEKDNKASS"
     gene            complement(16155..16859)
                     /locus_tag="PLG42_00080"
     CDS             complement(16155..16859)
                     /locus_tag="PLG42_00080"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOU29635.1"
                     /translation="MHIFYASGIKGEKCILDVTESRHCVKVLRLKKNDPVKLIDGTGN
                     LYQGVISEPDPEACTVRITKVIKDFQPRPYRLHLAVSPLKNPERFDWLVEKCVEIGVD
                     EITPVICKNTEKKNIKPERIKKIIISAMKQSLKTYLTNLNESIDFNSFISADFNGIKL
                     IAHCSANFTREHVSGIYKKGEDVLFLIGPEGDFTDDEIRAAEKMGFRSVHLGPSRLRT
                     ETAGIAACHSIYFLNQ"
     gene            complement(16880..21364)
                     /locus_tag="PLG42_00085"
     CDS             complement(16880..21364)
                     /locus_tag="PLG42_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29636.1"
                     /translation="MLKKLIITCLALIFASGSAFTQGRKVFSGEREKFSAELVAYMGP
                     NLTPDQSAILSKFTSSWDSAYFSSDIAENIMGISSLLAARNFRPVPHFIDFLKTLLDL
                     SQYKGNKGYFLTWLTGLVAMIHKPAINNDNIDRHLRNTSSFVTSGIVFESATVKWKIK
                     GQNTTFEYDTSFFINAQNVTLTAFSQKDSIEIYDVTGKYFPENTMFRGVIGTVYFEKA
                     GYPRNEAFAEIRNYSINLQRAAYEVDSALFTYKTFFEKPEYGKLTDQVSLSGNRENIR
                     YPRFETYTKTFAIKNIFKDVNYEGGIRLEGSTVIGMGDVFTPARITIFRNDTLYLKVA
                     ANTCLITRGGINTQESSATFYLGNDSIHHSSIGFSYNAENREINLFRSNNPIAKSPWF
                     DSFHGFDIYFENLIWNMNQSTVILSRSRGASLGQARFESASFFNSTYFERLAGIDEYH
                     PLYRLRQFADWFYSNTFPVKEFAKWMKKPEEAVTGLCIDLTNRGFLFYDRVNNEVTIK
                     SKVDDFINAYAKKKDYDVISFMSETNAPVENAFLDLNSFRLTVNGVRNIFLSDSQMVS
                     LYPYNQRIEIGKNRKISFDGVVEAGLFTIFGHNFVFDYDTFKINLHKIDSIRIAVETG
                     ERDAMGRPLIKDVENLIQLTTAELYIDDPKNKSGLRSLKQYPIINATTYSYIFYDKIP
                     GLEGIYKQQDFYFRIEPFTYDNIDHYTNEMMNLPGEFHAGNIIKPTKQYLIIQEDNSL
                     GFTMNIPSDGIEVYDGKGRLYNMISMSNKGLTGSGKLRRITSETESDEFRFFPDSMIT
                     FARSFTISPDGSGRFPVLSSNDVSVKWLTSTDEWIASNAPGKKFDMFGNGTQLDGKII
                     LTPERLSGSGIIDITDSRIISNKFNFAENIIQSDTADYNLKSTRGEAIGFLAENVNVT
                     IDFNSYQSKFSLNTGTSLVKFPEIQYICTMTDFQYDMKNRILNMEQRGKAETPLMSPE
                     ELLKLDFNKLDKPTFFATNIIKDTIAFTSWKGRYDLNQEVVIAENINYIHIADALIQP
                     DKGRITIERGARIQPLQNAVIAVNNKHLLHSGNIVIENTRSYSGSATYNYVDESGNIQ
                     QINFPKITSDKGITSATGYIPVTQNFMLSPAFTFTGDVMLSAQDDYLTFTGAAGIVHN
                     CSFKSYNVKFKSSINPKMVIIPIAEKVRDINDNPVYSGSFISTDSAHIYPAFLSERKS
                     WSDAQIVNAQGALWYEKEKGVYRIASMEKLADLSLPGNMITLDKNYCILSGEGQLNFG
                     ANYDHLKMAAAGKVIHNIDSGKVNFEAVIALDFYFSPQALEMMSNEIRRVPALAPVNL
                     NTEFYKKAMKDLLGESVATRINEEIGLFGTTRNLPREYPYELLLNDVKLYWNEPTSSF
                     RSKGKIGIGLIGSNAVNVYVDGFIEIQRRRSGDMLDIYLKADNSTWYYFSYFRGVLMT
                     QSSNLEYNTLISTLKEKVRTHPESSTRVPYTYMIAVEDRLPRFLQRMTSDQAEEQEQK
                     "
     gene            complement(21520..22278)
                     /gene="tpiA"
                     /locus_tag="PLG42_00090"
     CDS             complement(21520..22278)
                     /gene="tpiA"
                     /locus_tag="PLG42_00090"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOU29637.1"
                     /translation="MRKKIVAGNWKMNMDLSSGIELAKGVKEYFAGKTPKAEIVLCTP
                     FIHLSVMKDVLKDSNIKFGAQNCYSEPSGAFTGEISCGMIKSTGAEYVIIGHSERRTL
                     FKEDNKMLNKKVRMALSTGLKVIYCCGEILKEREQGIHFDVVKEQLTEGLFNLTEDEF
                     KNVVIAYEPVWAIGTGLNATPEQAQEMQSFIRKLIAEKYGKGISENTTILYGGSCKAS
                     NAAELFSMPDVDGGLIGGASLKKEEFCAIVEAVK"
     gene            complement(22303..23409)
                     /locus_tag="PLG42_00095"
     CDS             complement(22303..23409)
                     /locus_tag="PLG42_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOU29638.1"
                     /translation="MKILRVISRIITGAVFVFSGTVKAIDPLGSTYKFSDYFTAFGLD
                     FMQPLCLPLAFLLCLAEFITGFSLLTGIKYKAGVMGATVLMSIFTPLTLILAITNPVS
                     DCGCFGDAIKLTNWQTFWKNVVLMTLVIILIFDKNKPLEYIRSLPGWLVIFSVSIIFM
                     GFTGYNLKYLPIIDFLPYKKGNNIKELMEIPEGAPAPVYKTTFIYEKNGIRKEFTLDN
                     FPADDTTWKFIDQKSVLVKKGYEPPIHGFSLVTADNRDITDMVLNNSNFTLLMISPKL
                     EKAKPERLNKGFQTGHKCMTYGIDFYILTASSSDKIKDYDSNLQFCLVDEITLKTMVR
                     SNPGYIMLRNGTITGKWSWANLPEDIENLIKTNN"
     gene            complement(23411..24097)
                     /gene="tmk"
                     /locus_tag="PLG42_00100"
     CDS             complement(23411..24097)
                     /gene="tmk"
                     /locus_tag="PLG42_00100"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOU29639.1"
                     /translation="MKLIVIEGLDGSGKSTQIKLLKNFYEARNKKCEFLHFPRTDRPW
                     FGELIARFLRGEFGSLNEVDPYLVAMLYAGDRKDAAGMIKSWLNEGKVVLLDRYTYSN
                     IAYQCAKLNDEKKQEELKKWIFELEFEYFKIPRPDINLFLDVPFNFVESKLSTRRKGD
                     DRKYLNGKNDIHESSLAYQMRVREMYLKVAETDPLVEVVNCSDTEGKILPPDIIFRII
                     INILEQKNLT"
     gene            24196..25053
                     /gene="folP"
                     /locus_tag="PLG42_00105"
     CDS             24196..25053
                     /gene="folP"
                     /locus_tag="PLG42_00105"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOU29640.1"
                     /translation="MLKKSEYINTGGKLIDLKIPRIMGIINVTPDSFYTGSRYFDDKS
                     ILEAASRMLDEGADFIDVGGYSSRPGAEDITPEEEKSRVLRAIKLITREIPEAIVSVD
                     TFRAEIAYEAVMECGAKIINDISGGEADKEMFNVVAELNVPYILMHMQGTPRTMQINP
                     AYNDVVADILMWFGERIFKLRTMGVKDIILDPGFGFGKTVEHNYELLRRLGEFKIAGL
                     PIMAGLSRKSMIWKTLDVKPEDALNGTTALNMAALMNGADILRVHDVKEAVQVRTLYL
                     KLRLINKEG"
     gene            25113..25886
                     /gene="cdaA"
                     /locus_tag="PLG42_00110"
     CDS             25113..25886
                     /gene="cdaA"
                     /locus_tag="PLG42_00110"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOU29641.1"
                     /translation="MPFHITIYDVIDIFLVAFLLYQLYRLIKGTPALSIFVAIFLIYL
                     FWLLVRVLKLELVSTLLGQVIGVGVIALIIVFQQEIRKFLIVMGSRYVYNNKLLGLKK
                     IFPENMVQKGHKNTEEIIRAVEYLSNNKIGALIVIGRSGLLEPYSEGGEKLSAAINAS
                     LLETIFTKNTPLHDGAVLIENGKIIAARCPLPITEQVNLPEEFGMRHRAALGMSEHTD
                     AIVIVVSEETGKISVADSGVIRSNISIKDLRQILANERI"
     gene            complement(25897..26325)
                     /locus_tag="PLG42_00115"
     CDS             complement(25897..26325)
                     /locus_tag="PLG42_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29642.1"
                     /translation="MKKYALYIIMLLFSAILSCDENPIFIDCNDCMPDEPDHATLVLY
                     MEKQLTDWHPAAIVRIYQGNLEDNVLLNLFYIHYEKWEIDVPLNKKYTITATYTDING
                     KDYIAVDTAYPRVKYEKSQCENPCYFVYERIVNLRLKYMK"
     gene            complement(26333..26764)
                     /locus_tag="PLG42_00120"
     CDS             complement(26333..26764)
                     /locus_tag="PLG42_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29643.1"
                     /translation="MRNFGVLSGIDFKPAYTRVIIKKEGDVYYQYMDKSYLFYAGLFK
                     DFRLTDYPLRGNWTLTASLSGGYFFANRLKGTNETPGGKIKILPAAGLKWTKNNLLLK
                     AELEYFRTPFYKIGPLWITAGIGYTIYFNKIRAPGKYIRWY"
     gene            complement(26966..27802)
                     /locus_tag="PLG42_00125"
     CDS             complement(26966..27802)
                     /locus_tag="PLG42_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU29644.1"
                     /translation="MPLKLYLYLRIVAVLLIIASHTNISSSQENKRLEEEDDTLRYIS
                     LLRGPEAELNLNYALLTAAAEGKINSIRWLIKYGADVNTSTMEGVTPLHFAVGNDKTE
                     AVKVLLEYGPKLDVLSNYSESPLHIAVKKNNTEIAEILARAGADVNIRDRFGATPLHY
                     ACAYGYFSAADMLLYYDAAIYTKDNEGTTPLMAAVWAGHANIADLLLQNGADPSDRDN
                     DGFTPFLIAAQNGDTLIMDLLIKRFVNIYETNNYNYNALDLCIMSNHKEALEYLLRKG
                     EK"
     gene            27906..29213
                     /locus_tag="PLG42_00130"
     CDS             27906..29213
                     /locus_tag="PLG42_00130"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU29645.1"
                     /translation="MVRKSLKILFILSIILLAGYHPEAVEKNIYLKEKLVPGNIRLTN
                     DFSEAPEFENFERVIRYFMRRWDLVGVSVAVAKDGKLVYAKGFGLADSTSKEQVQPYY
                     RFRIASISKLITAVAIMKLQEEGKLSINDRVFGPDGILNDPYYSNPKDKRIYGITVAH
                     LLAHEGGWTTRFGDQMFMPFVVAKEMGVPVPVDTKTIVRFALNKNLHFTPGTGRSYSN
                     LGYAILGLVIEKVSGLSYSEYCRKNLFEPYGIYDMYLAKNLKDEKLPFEVTYYVPPDA
                     ILKPSVYGNGELVSPAYGGNDIEALGAAGAWVATAPDLLKFVLHIDGLKGRKDILHEE
                     SIRFMTDNNNKYAPIGWKGTINNGVWWRTGSFPGTAGMVKRQPDGISWVFLTNTSTWN
                     GPEISTEINIFMTRALSQVRKWPERDLFNYYVEAPLSSDITIE"
     gene            29226..29951
                     /locus_tag="PLG42_00135"
     CDS             29226..29951
                     /locus_tag="PLG42_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOU29646.1"
                     /translation="MCFTVNVNIVKEELEERYHARLIDPENYRPSYYYHAFSIPKLPV
                     VHTGNREFIEHMNWGLIPSWVRDREKADEIRYKTFNARAESIDAKPSFSNSFATRRCI
                     IPVKGFFEWQHVGERKIPWYVFRADEDIMSLAGLWSEWVDNTTGEVVKTFTVITTEAN
                     EMMSKIHNSKKRMPVVLERSSEEIWLDLKVPKDKLLKLLEPYPDKILKAYTIGTLINR
                     KDADKNSADLIKPVDYNLPGKLL"
     gene            complement(29972..30712)
                     /locus_tag="PLG42_00140"
     CDS             complement(29972..30712)
                     /locus_tag="PLG42_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29647.1"
                     /translation="MNGREKLVGILGAVIIHLTAGIIFMIVKIGSLDIDKYMREYEII
                     FLEENLPEEFAPKQSVGINDDNQAVDQEILNIARNLAAKPDFKINPEDYIDRVKEEMI
                     KSGLLGPDNYIDEQKRLKAESSDRGIEVQKDEEDRDEKMRESNEMAARYSGPTRIYYY
                     LPGRTHVYLPLPIYKCEGGGKIVLAIEVNPKGEVVKAAIIAGESTTSDPCLTEAAIIS
                     AYSSRFNPDATASKNQTGTLTYHFVPQW"
     gene            30777..31730
                     /locus_tag="PLG42_00145"
     CDS             30777..31730
                     /locus_tag="PLG42_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOU29648.1"
                     /translation="MPVEIIVNQILILAIVALVGVIASKAGVITSEGKDFLAKIIFNV
                     TLPSMLLASFGSIDATPRLLTNSLEFIGLSFFSIMLLFIAGTAASSILKLKGEESAIF
                     RIHSMMGNIIYLAFPVIYSLYGQEGILYGSIFTIVSNVVLWTLGIVIIAGNSKISVKE
                     SFKYIINPNTIAILTGFCLFLLHIKLPKIIIDSVGGIGSTNTYLSILYIGSVLFYSKT
                     GRYLKNINIYVLSFNKMILVPFLLTGIFWLAAAYLNLHFDKLVLSVLILESAMPCMVN
                     VVIIAKILGMDDAPAVANVFVSTILSIITLPIVLIFLSVLK"
     gene            31748..32329
                     /locus_tag="PLG42_00150"
     CDS             31748..32329
                     /locus_tag="PLG42_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU29649.1"
                     /translation="MGKLSVLEVIRDRWSPYSFSSIRVEEEKFELLFEAARFAPSSMN
                     EQPWLFIFTTKDDGKYFEIFLDFLYEGNRIWAKNAYVIGIVMAKTRFSKNDSPNRHSL
                     YDTGMAVGNLLAQATSMGLYVHQMGGFSVDKVKKHFGADDYIEPVAMMAIGYLGDGTD
                     LSDELKVRDMKRRPRKALNEFVFRNELSNPAFK"
     gene            complement(32331..34757)
                     /locus_tag="PLG42_00155"
     CDS             complement(32331..34757)
                     /locus_tag="PLG42_00155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU29650.1"
                     /translation="MVRLPKIIIQAVIPLVFLSNSYSTAQDLIVIRGVVKDTTGIPVE
                     NATITISNYNTGIYTNNHGLFSLSIPKDGKEHILSISVTGYERKAITIVCNYNIDLGE
                     IILSRKVTGLDEITVSARSKQSGAGMISISPKDFSMMPSASGSFETVLKTLPGVSSNN
                     ELSSQYSVRGGNFDENLVYINDVEIFRPFLIRSGQQEGLSVINPDMVSSVLFSAGGFG
                     VTYGDKMSSVLDITYRRPDNLKGSVSAGLLINTAHFEGISRSGKITWLTGLRYKSSRL
                     MLKTLDSKGDYLPVFADIQSIVTIKTGNKSEISLFGTYSSNTYNYVPRSRESKFGNET
                     EAYRLYVVFEGREKDRYQTWNTALTWKYSGIKSTHKIIISNFGTNEREQFDIRGFYSL
                     NMLDRNMGSENMVDTLLNIGVGSFLSHARNKISARFFTIKYSGFMNASDNLSFRWGLK
                     ANNEIINDNIREWKMVDSAGFSIPWNTNGELRVSSMLKGSNRIINRIYEAYGEARATL
                     RGEKYNTILTGGIRSTYNSFTSEFFACPRFSARVEMEKGISAWLASGAYIQPPFYREM
                     RFPDGTLNKNIKSQKSFHVVTGMSYDFKAWERPFRFTAEIYNKKLSGIIPYRLDNVRI
                     IYEGDNMATGYSRGIDFRLNGEFVEGAESWFSLSIMDSKLEMPGIIEDMFPSPSEQTL
                     NCNIFFQDYLPGNPSYRAHINISFVTGLPVISPFNSRYDQYHRLPPYRRVDLGMTKVF
                     NNFSSSGFMSGIEELIVCVEIFNLLDINNTVSYFWIKTVNNQSGRSRQFAIPDYLTGR
                     SLNLKFTAYF"
     gene            complement(34758..35408)
                     /locus_tag="PLG42_00160"
     CDS             complement(34758..35408)
                     /locus_tag="PLG42_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU29651.1"
                     /translation="MVSLRVLEAQESQKYKLIQASGVITNRENEPVYNASVFSQKLRR
                     GTISEVTGIYSIISMPGDTIIISALGYKKYSYSIPSDFDGRIYKQDVMLVADTISIEG
                     ITIFPWKTYEEFKREFLATRPVIKPEIQYMYENLYLIQESINQTGAYAVTPEAGFRMA
                     MQQNANAIMTRNQNPANNLLNPFAWAKFFSGLKSGLLKNEKSEEKSGSKTKARRQK"
     gene            35645..37267
                     /locus_tag="PLG42_00165"
     CDS             35645..37267
                     /locus_tag="PLG42_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOU29652.1"
                     /translation="MQRLLLLGDEAIAQGAIDAGISGMYAYPGTPSTEIMEYIQASPE
                     GKSGRIECRWSSNEKTAVETALGMSYCGKRAMAAMKHVGLNVAADAFINSAITGVNGG
                     LIIVAADDPSMHSSQNEQDSRFYGKFAHVPVYEPRNQQEAYDMVFDAFELSEKYSLPV
                     LMRIPTRLAHSRSGVVKRRQIPEKKFRLPDDSRRFILMPAIARKRFKILLENYEKLQF
                     DNRLTKYNTFKDGPDRSLGIIACGIAVNYLMENFSDDELPYPLLSVSTYPFSKDIIKD
                     ITGRCREILVLEEGYPLVEEQLRGILDEGRRIYGRMDGTIPRDGELNPDIVASALGIK
                     AGKGHTVPEIVKSRPPSFCKGCGHADMFNAIVEAIKPYGIQGVFSDIGCYTLAALPPY
                     SCINSCVDMGASVTMAIGAADAGLFPSIAVIGDSTFTHSGMPGLLDAVAKNSPVTIII
                     ADNLTTGMTGGQESNAIGRLQSICTGIGVAPEHVRTIVPLRRNHEENVRIMREEFEYR
                     GPSVVIATRECIQAGARRRKSEKSEKQKNNEE"
     gene            37264..37842
                     /locus_tag="PLG42_00170"
     CDS             37264..37842
                     /locus_tag="PLG42_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOU29653.1"
                     /translation="MKKDIIIAGVGGQGILSIAATIGLAAVEKNMFLKQSEVHGMSQR
                     GGDVVSHLRISDKPIHSDLIPYGTSDLIISVEPMEALRHLPWLAPGGWIVTNSNPYVN
                     ISDYPPVDEIIKNIKKVKNSIIVDADSIAADCGSIKAANMVILGAASPFMGIPLENLE
                     DALKKMFSKKGEQIVEINLKAFRSGRKIAEVQ"
     gene            complement(37844..38794)
                     /locus_tag="PLG42_00175"
     CDS             complement(37844..38794)
                     /locus_tag="PLG42_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29654.1"
                     /translation="MTIAEFQIFSQQEEKIFTPSAEIFTDFHFSITDNTHKPEFSITR
                     AYFGGDFLKEGRFSGSIILNMAKLFDSIPHEGNNSPIREVSIGYDNGKLKIRMGVTGT
                     RIMIYQIRFYDKRYVANTFQSINKYGSLYDLGIAIDYNISNRTGIDFTLMNGGGVAIN
                     DHGSLKASFGFNYFPANDAILRVYCDTRKIAGIWQSTFIGFGGFTWNVFKIGGEFNYR
                     TSHDNTRGHNVWGFSATGSVNLTDKLQLFGRYDYSSSVVLPGEIIEWNYKNDHQLIVF
                     GLQHTFIPGVRIAIDYQGKIPADPSESASDMFFIHLHYRY"
     gene            38914..39414
                     /locus_tag="PLG42_00180"
     CDS             38914..39414
                     /locus_tag="PLG42_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU29655.1"
                     /translation="MKKVVLSILTLLAIGIYTIQMAEAQSNVKLYDPSLDGMKQIREA
                     VAQAKKSGKHVFIQYGGNWCPWCIKFDAFVKSDTGLTRIINQHYVPVKLNYDQTNRNY
                     EANEYLGNPIRFGFPVFIILNGDGKVLHIQDSALLEEGEGYNRQKVYSFLNNWTPSAI
                     IPVRKQ"
     gene            complement(39498..39860)
                     /gene="ssb"
                     /locus_tag="PLG42_00185"
     CDS             complement(39498..39860)
                     /gene="ssb"
                     /locus_tag="PLG42_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU29656.1"
                     /translation="MNQMKNRVLLIGNLGHDPETRTLDSGKRVSRFTLATSDTFKNSE
                     GQKVTETTWHNIVVWNRLSDFASKYLKKGRQVYVEGRIAYRSYEDKTGVTKYTTEIVA
                     SNLLCLSPAGSPVKQAAE"
     gene            40026..40391
                     /locus_tag="PLG42_00190"
     CDS             40026..40391
                     /locus_tag="PLG42_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29657.1"
                     /translation="MERRCLDCGDVIHGRSDKKFCSDQCRNNYNNKLNRDTNNYVRNV
                     HGLLRKNRRILCDLYNEGKRVIHRDALFALGYNFNFFTHIIQTRQGDKYHYCFEYGYR
                     EKDDEYIELTQNLQYLEYI"
     gene            complement(40406..40951)
                     /locus_tag="PLG42_00195"
     CDS             complement(40406..40951)
                     /locus_tag="PLG42_00195"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOU29658.1"
                     /translation="MNSRIFLLLIVLVIGASSCTVSKESYSLRAEKRKAERLAAQVAI
                     KKAIESRRFVLKMDKIILPDGLMVDLVPRNNFIIINGEIISVSLGYAGRSFGARQISG
                     INFNGRTGRYLMKSDEAKGIYNIQVEAITPNKDKFDIYLTLGENGYCSASVNNPYIQS
                     VSYRGILVPLSPENVSYSIRK"
     gene            41275..41889
                     /locus_tag="PLG42_00200"
     CDS             41275..41889
                     /locus_tag="PLG42_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU29659.1"
                     /translation="MKKLFVLLFVSLMAIPAFSQIKFGIKAGAATATVPKYDLSTGTT
                     NIEAVKDAAWGFHGGLFLRLTLLGINIQPEAYLATNTYEYKVTSGTLPEIKKQTFNRL
                     DIPVLVGFKLGPVRLNAGPAAAIQIGTPKALIDDPNFEEMYRGATFGYQAGLGIDLFK
                     KITLDARYEGSLSGKFGEAVTIGGQTFKLDQRQPAFVLSVGLIF"
     gene            41969..43438
                     /gene="cls"
                     /locus_tag="PLG42_00205"
     CDS             41969..43438
                     /gene="cls"
                     /locus_tag="PLG42_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOU29660.1"
                     /translation="MTEVAEMGFPWFQFLIISFYLVTALFICFMIIFENRAPVKTLSW
                     VMVVLLVPVAGIIFYLLFEQNYRKQKIFSRKSVLDMEQLSNIAAFQVKTLPERLKTAS
                     EKVREKEHLIKLMLNNSKSVLTEFNKVDILIDGIETFPSMFTAINEAKSFINIEFYRF
                     ESDILGMKFCDALINKAKEGVKVRVIYDDVGSWNLSKKVVKRMRESGVEIFPFMPVRF
                     PWLTNKINYRNHRKILVVDGIKGYVGGLNIADKYLHGHREIGNWRDTHLLIRGEAVTA
                     LNYVFMVDWYFVSDILLTDEEPIDDKYKVADRCWIQVASSGPDSDWANIMQVYFSAIA
                     TARKYIYLCTPYFSPDETILNALLTASLSGVDVRLILPGKSDSPVSYWNTRSYISEMM
                     DAGIKVYLYNDGFNHSKYLLVDDVFASVGSANVDMRSFDLNFEIAAMIYDEDFAARLL
                     KVFYIDVARSIEVKPDEWNKRKKTERYKESIARIFGPLY"
     gene            43454..>44312
                     /locus_tag="PLG42_00210"
     CDS             43454..>44312
                     /locus_tag="PLG42_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29661.1"
                     /translation="MLKEGEEYIFTAGKEIETPDGKLYRILQGPDNVKYLLRADYYKE
                     YEITEGKRITCRVDKINCRGEVFLEPRHPVYEPGKQYKFKVVSHEFRSDDSGNDTAVI
                     LVEDVFGNKIALPSGVQNTELPASGSEILLTVERIFRGRPVLYRPSSEKGRFKGLKYG
                     EYYDFRIEKVSKGIDGEEIFVVSDPFGRVHTIPVKYFSHYNINPGSVFKGRIIKHSRN
                     SSWKIEPRNPYYNKDDIISLKVESVMPGVNENSVILTLKDEFGFVHLVNSKTKPENKL
                     VKCRVKTIRK"
BASE COUNT        13425 a         8225 c         9147 g        13515 t
ORIGIN      
        1 cagggttaat ataattataa ctcattggga agaaataatt taaattgaaa aaggttttag
       61 ttttcaatta taaaaataat atttatttca aaattcagga atttcgttat taatttattt
      121 ccggttgctt atactgtcga ccttataact ccttttacca gcagataaaa tgaatataaa
      181 attctgcgag aaaacatttt attgtttaat aagatgttac ttatagttta ttttttaaac
      241 aataaacaga aaatcaaaca gttttttatt tgaaataaat attaaattta gcgtgcaaaa
      301 ttcctttcaa aatgagacta acaggatatt cattgttgct tattttattg attctcacct
      361 gcagatcagc aaatgcccag acaaatgtgg aaatagaaaa gagccagttc agaaatgtca
      421 gggatggatt taaagtcgca tgggagcatg tgaaggaggg cgatgcatat tttatggaag
      481 gtggggtatt gtacagaaat gcttttgaag agtataaaaa agcttacgca tataattcaa
      541 caaatgcgga attaaattac aaaatcggag ttgctgcctt attttcagat aatcaggata
      601 aggcagccga atatcttctt aaagcctatg aaattaataa taaagtagct gaagatgtgc
      661 tcctgctggc agggagagct cttcaatatg ctggcagata caatgaggca atagaaaaac
      721 tggaattata catcgactct tccaacaaaa gaaataaagc tggagtggag atggcaaaaa
      781 aatacattga ggaatgtaag tcagctattg aattaacaaa agatacattg agaattgaaa
      841 tccagaacct tggtggcagt gtgaactccg attgtgatga ttacagtata gtaccgacag
      901 gggatgggaa aagaatatat tttggctcga gaagagctac atcaggttct tcttcaaagc
      961 gttatgatga taagaagttt gatgaaaata tttttattac tgattttcag aacggtgtct
     1021 ggacaaatgc attacctgtt ggaagtaaaa taaatactga atattgtgaa actcctcttt
     1081 ttctgagcaa agcagaagac aggctatttg tttatgcggg ttatgaagga aacggtgata
     1141 ttatggtttc cgaattgaga agggggtcgt ggagggcgcc ggtaaaagaa aaaataggga
     1201 tttcaagcaa ctttcccgaa acatcgatgt gtatttcacc cgacggaaat gaagttgcct
     1261 ttatacgcga tgcaggtaaa aaaggtatag gcgggaaaga catttatatt gttaaaagaa
     1321 ttcaggataa aaggtggtca aaacctcaaa atgcaggaat aacagttaac agccggtacg
     1381 atgaggaatc tgtaagatat tcgaaaggag gtgatacatt gtggttcagt tctgcagggc
     1441 acaatgcaat tggaggattt gatatatttt acagtgtaag aagcaccgat ggcagctggg
     1501 gtcctgctat gaatgcaggc tttcctataa atacgccatg gcatgagctc ttttatgtcc
     1561 ctgcccccga cgacgacagt gcattttatt ttgtctccaa ccgcaagggg ggatatggtg
     1621 gtctcgacat ttataagggt cgcctgctgc ctcctcctcc ggttgtaact cctcctgaac
     1681 ccgaacccga acctgtaaag ccacctgaac cacagattgt ggtggtacgc gatacggtag
     1741 tcattatcaa ggaagttgca ccacctgccc cggtcattga caaatcagtt taccttactg
     1801 gaaatattac cgactcagaa acaggtgctc ctgttcttgc acgcattgaa gttattgatt
     1861 accttacaga tgcagttatt gccacaacag caagttctga tgctgacggt tcatataaag
     1921 taaaactgcc tgaaaggaaa atatatatag taaacgttag agctacaggt ttcctgtcgg
     1981 atatgaagaa gatatcaata cccgaagatt atccttccga tttaatgagg cttgatgttc
     2041 aacttgcaaa ggtcaaggtg gggaagaaag ttgttctcaa caatatcttc ttcgaaacag
     2101 ggaaagcagt gctgactaaa gattctttcg aagaactcga taaactggtg ggtattatga
     2161 atgataatcc ctcgatgaag attgaaatat caggccatac tgataataca gggagtgctg
     2221 tgataaatgc ccggttgtca acagaaagag caagagcagt tgttgaatat ctggtgagca
     2281 aggggataga cagagcgaga atgacatata ggggttatgg acccgaccag ccaatagctg
     2341 ataattctac tcctgaaggg cgagccaaaa accgccgtgt ggaattcaaa atacttgaaa
     2401 tgtaaactga atgagaattt catgacttta agcatgaaat tttattaatc agtgacttat
     2461 ttttgactta agtttttaca attttccata attatgaaac atactccatt tacgcagatc
     2521 cacattggtc tgggtgcaaa gatggtacct tttgccggat tttacatgcc tgtagaatat
     2581 acaggaatta tcgatgaaca cattactgtg agggaaaaag ttggggtatt tgatgtgtcg
     2641 catatgggtg aattcagagt caaaggaccg aatgcattcg cttttttaca gtatattacc
     2701 tcaaatgacg tttcagcact gtacgacgga aaggttcagt attcatgttt ccccaacggc
     2761 aggggaggaa tagtggacga cttgctggtt tacaggttca gttccgatga ttatcttctc
     2821 gtggtaaatg ctgcaaatat tgaaaaagat tggcaatggt gtgtgcagca cgccagacgc
     2881 ttcggtattt tacctggaaa ggaaattatt aacctgtccg actccacagc acagttagct
     2941 gttcagggac cacttgcact aaaggcaatg cagaaattaa cctctgccag cattgaaggc
     3001 atggaatact atactttcaa ggttattgaa tttgctggaa taaaagatgt tattttctca
     3061 accacaggtt atactggtgc aggtggatgc gaaatatatg tgaaaaatga agatggaccg
     3121 cggttatggg aagctgtttt tgaagccgga aaggaatttg gaatcaaacc tgcaggactt
     3181 ggtgccagag atacactgcg tctcgaaatg ggctactgcc tctacggaaa tgacatcgat
     3241 gatacaacct cccctataga ggcagggctt ggctggataa caaaatttgt tgacggcaaa
     3301 gactttatcg accgggaact tcttcttaaa caaaagaccg aaggcaccag ccggaaactc
     3361 agaggttttg aaatgattga aaaaggaata ccaagacaac attatgaagt gtttgattcc
     3421 gcaggaaata aaataggtga agtgacatca ggtacaatgt ctcccatgct gaaaaaggga
     3481 atcggtatgg catacctcac cagaggattc tggaaacctg gtactgaaat attcattaga
     3541 ataagaaata aagaccttaa ggcagtaatc gttgaactac cattttataa aaaacaatag
     3601 gcttgtaata aatgatctat tgattttcat cattcctgct gaaaagagat aaatatgtaa
     3661 ttttgaaatg ctggcttaag ctccggaata atggaaaaac tcaaacttga agtttgcctt
     3721 tctcctgcaa tgttccacag gtatgattgt agaggcaata ttgtggttat tatagatgta
     3781 ctgagggcat gttcggcaat ttgcactgct tttgaaaacg gcgcggctga aatcattcct
     3841 gttgcagaag ctgaagaggc aaaggagtat aaaaacagag gttatctggt agcagctgaa
     3901 agagatggtt ttacactcga ttttgccgat tttggaaatt ctccttttaa ttttacacgc
     3961 gaacgtgttg aaggcaaaac aatagcttat tcaacaacca acgggacgag gattatagaa
     4021 atgactgata aatcatacaa aactattata ggagcttttc tgaatatttc ttcggtaaca
     4081 gaatggattc tggcagagaa gaaggatgta attcttttct gcgccggctg gaaagacaga
     4141 ttcagtcttg aagatacagt ttgcgccgga gcatatgcat cgaggctcct tgaaagtgga
     4201 ctctttacta ctatttgtga ttccgttcat gcttctctgg acatatggga gatagccagg
     4261 aatgatctga gaggatatat tgagaaagca gctcagagaa gccgtcttcg cgataaggga
     4321 cttgacgatt gtattgattt ctgccttact gctgatatta caccagttat accggtcgtg
     4381 gagaaaggga aaattgtacc tttgagatat aaatagaaaa aaggcagaat taaatagaca
     4441 gggacaggaa ggtagtatga aattttaaga aataaataaa ttgaaatatt attgcgataa
     4501 aagaacagta ttaaaataaa cttcaaacta tgaaaaagca caatttttac gcagggcctt
     4561 ctattcttcc gcaattcact attgaaaaga caatagaagg aatcaaaaat tttgccggaa
     4621 cagggttgtc agttcttgag atatcccacc ggagtaaaga gtttatagca tgtatgaatg
     4681 atacaatagc actggttaag gagctgcttg atgttcctca gggttatcat gttattttcc
     4741 ttggaggggg tgccagcttg cagttttgta tggtgccgat aaatctactt aagaataaag
     4801 cagcatatct tgtaacaggt gaatgggctt ccaaagcttt caaggaagca aagcttattg
     4861 gtgaagcagt tgaagtggca tcatcaaaag acaggaattt ctcgtatata ccgaaaaatt
     4921 acaccattcc tgccgatgcc gattattttc atataacaac aaataatacc atttacggaa
     4981 ctgaacttaa ggaggatcct gattcaccgg tacctgtggt tgcagatatg tcatccgata
     5041 ttttcagtcg tccggtggat gtttcaaaat atgcaataat ttatgctggt gctcagaaaa
     5101 accttggacc ggcaggtgta acaatggtta tcgtcaggga agatgttctc gggaaagttg
     5161 agcgtaccat ccccacaatg ctcgattacc gtacgcacat aaaggcagaa tcgatgttca
     5221 atactcctcc ggtgttcgcg gtatttgccg cacaacaaac ccttctctgg ctgaaagaaa
     5281 tgggtggtgt aagagttatg cagaaacgaa acgaagaaaa agcaaaaatt ctttatgatg
     5341 agattgaaag gaataaattg ttcatgccca cggtaagcga tcctgccgac cgttcaatta
     5401 tgaatgtatg ttttgtgatg aaacccgaat acaaagaact tgaagtagag ttccttgatt
     5461 ttgcaaaatc acgagggatg gttggtattg caggacacag gtcggtaggt ggtttcagag
     5521 cttcacttta taatgctctt ccaaaagaga gcgttgaggc tctggtggag gttatgaagg
     5581 aatttgaatc caaaaaatag aatcatgaaa atattgctgg caacagaaaa accattttct
     5641 cctgctgcag ttagaaaaat gcgtgagctg gtagaagctg aagggcatca gtttgttacg
     5701 cttgagagtt acacgagtat tgaagaatta ttacatgctg ttgccgatgc cagcgcactg
     5761 attgtaagga gtgatccagt tactgctcag gttctcgatg ctgcacctga attaaaactt
     5821 gtggtgaggg ctggcgccgg atacgataat atcgacatca aagcctgcac agcccgcaat
     5881 gtgattgcca tgaacacacc cgggcagaac tccaacgctg ttgccgaact ggcattcctt
     5941 atgatgcttt atatggcacg ttcagggttt gcaggaaaat ggggtacgga actcagaggc
     6001 aagtcgatag ggttacacgg atttggaaac gtcagccgtt ttatgaacca gatcgcaaaa
     6061 ggtttcggca tgaaggtata tgcctacgat ccgtatgttg aggaaaatga gatgaaacag
     6121 tcaggcgtca ttccctgcaa aactcctgaa gaactttaca gcaattgtca gtatgtgtca
     6181 ttgcatattc ctttaaataa taaaacccgg aaatctattg gctacgaatt actgaaactg
     6241 atgccacccg aagctgtgct cgtgaataca gcaaggaaag aaataataga tgaagatggc
     6301 ttgctgaaaa tattcgacga acggtcagat attaaatatc tttcggatat tgaacctgac
     6361 tgtaaagcca tatttgaaca gaaatacagc ggaagatttt tatttacggc caaaaagatg
     6421 ggggctcaaa cagaggaagc taataccaat gctggaattg ctgctgtaaa acaaattatt
     6481 gattatttca aaacaggaaa tgataagttc agagttaact gaataatacc ttatatatta
     6541 tatatggctg ttgttaaacc tttcaggggt ctgagaccac ctgcatcaat agttaaggac
     6601 cttgcctgtc ttccatatga tgtaatgaac acagaagaag caaggcaaat ggcaaagggg
     6661 aaggagtgtt cattgcttca tatcacaagg gctgagatag atttgcctga aggaactgat
     6721 attcattcca gagaagttta cgacaaatcg gttgagaatt ttagattgtg gcgtgaaaga
     6781 ggctggctgg ttcaggataa agagccacat ttttatattt atgcccagac catgggagga
     6841 agaactcaat acggtattgc cggttgtgct tccgttgacg actattataa cggaattatt
     6901 aaaaaacatg agcttacccg gcccgataag gagagggaca ggatgattca tattaaggta
     6961 aacaatgcga acattgaacc agttttttta gcctatcctg ctgtagatga gatagataaa
     7021 attgttacgg aaataacaca aaaacagaag cctgaatacg attttatttc agaagatgga
     7081 ataggccacc atttctgggt gatatgggat aaagatacaa acaggaagat tgaagaactt
     7141 ttccgaacaa gagtaccata tacttacatc gcagatggtc atcaccgtac agctgcagca
     7201 gctcttgctg gcaaagaaaa aagggaaagc aatcccctgc acaggggaga tgaagaatat
     7261 aacttttttc tggcagtcca ttttcctgat aaccagttga aaatactcga ctacaatcgg
     7321 gttgtgaaag accttaacgg gttaacacca aaagaattaa ttgaaaggat aaaagcgagt
     7381 ttttctgtag aagataaggg aacagagata tatcatcccc ggcatttaca taatttctca
     7441 atgtacctcg atggcagatg gtacagcctc acagcaaagc agggtactta taacgatgat
     7501 gacccggttg gagtactcga tgtcaacatc cttacaaacc atattcttgc cccggtgctt
     7561 gggattgaag acctgaggcg ttcaacacgc atcgattttg ttggcggtat aagaggactt
     7621 gaggaactga aaaagagagt cgacagcggc gaaatgcagg ttgcttttgc tctttatcct
     7681 gtttccatta aacaacttat caacattgcg gatacaggca acataatgcc cccaaagaca
     7741 acatggttcg aacccaagct gcgaagcgga ctggtgattc atctgcttga ctgaaataga
     7801 atttatatgg atattgcatt aaatattaag cggttaaggg atgaagtccc ggaacatgtg
     7861 aagatagttg caatttctaa aacacgcccg gcaagcgata taatgattgc ttatcaggcc
     7921 gggcataaaa tattcggaga aaacagggca caggaattac tttcaaaaaa agatctgttg
     7981 ccacaggata tcgaatggca tttcgtaggt catcttcaga caaataaagt taaaatggtt
     8041 gtgccgaatg tgaaactgat tcattcggtt gacagcttta agttattgaa tgccattaac
     8101 aaagaagcag caaaattaaa tctttcagtt gagtgcctgc tgcagttcca tattgcacgg
     8161 gaggaaacaa aatcgggatt tacttttgag gaagcactta gaatgcttaa ttctcaggaa
     8221 atcaaagaaa tgaacaatgt gataatcaga ggggtaatgg ggatggctac ttttaccgac
     8281 gacatgaagg tggtcaggaa tgagttcaga acccttagaa aatattttaa catattaaaa
     8341 gatagatatt tcattgacaa taagtatttc actgaaatat caatgggtat gtcgggcgac
     8401 tacagaatcg caattgagga aggaagtact attatacgcg tgggaactct tatatttgga
     8461 gaacgtaatt attttgaaaa cgattaaaca tataatatga gcaagctcag tacaaaatac
     8521 ctgggactgg agctgaagag tcctgtaatt gctggtagca gtggtcagac gtcatctctg
     8581 gaaaaaataa aggaagcaga gaattgcgga gctggtgcaa tcgttctgaa atcactcttt
     8641 gaggaacaga tagaacatct tatagcttct tcaggtggtc cacactcata ccctgaagcc
     8701 gaggattata tggcgtatta cataaaagcc aatgcggttg aaaattatct gaatcttatt
     8761 tctgctgcca aaaaaagtat tagcattccc ataataccca gtattaactg tatatcagcc
     8821 ggttcatgga ttgattatgc gaaaatgatt gagaatgcag gtgctgatgc tcttgagatc
     8881 aacatctttt tcttaccggt aaaaggtgaa accacaggct cagagattga aaaaagttat
     8941 ctgcagataa ttgaaaaact caaaagcaaa gttaaagtac caatagcagt aaaaatcggc
     9001 ttcaggttta ctaatattct gaatattatt gaattacttt acaagagggg agtggaagga
     9061 gttgtaatgt ttaacaggtt ctacgaaccg gatattgaca ttgataaaat ggcagttgtt
     9121 cctgcagagg tattcagtct gccggctgaa agacgctatg ttctcagatg gattgcaatg
     9181 gctgatgctc ttgatttcaa aattgatata tcggcttcaa caggtattca tagtggtgat
     9241 gatgcgataa aatatcttct tgccggtgca gatactgtac aggtatgctc ggcactttat
     9301 aagaaaggca ttggttttat aaaagaggtg aacgaacgga taatcagctg gatggaaaaa
     9361 aggggattta attccattaa tcagttcaga ggaaaactca attattcagc tgtcgagaat
     9421 ccgatacttt acgaaagggc acagtttatg aaattctttt catcgcacga ttagaaattt
     9481 aaataatggt ataattccac ctttagattt ttcattgtac ccggtgatat aaatttttaa
     9541 ccactaatga tgcattagaa ctatcaaagt gatactgaat caataacata ggttgactaa
     9601 aagcaaagaa ggaatagcac attttctata aaaatttacc ataaatttta agttgaaata
     9661 ttcaataaat tttcttgaaa taaaaaagat attttaacct tttttgaaaa ataaaaatat
     9721 tgtgctaatt tagtcgttaa ttagttggta acttaaactt tttgatcatg aataaattaa
     9781 tcagaacagg aattatcaca gttataattc tgtcattatt tgttcttccg gggtgcaaat
     9841 cgggaggtga aaagcagaaa aaaattgaac ctgtaactca gaaggatgtc cagaagatta
     9901 aggaggatat aagcaaggct gaaaagatgt ttaatgcact tccttcgccg cttgaatcgg
     9961 caatgctcat aaaaacagcc ggtgcttcat tcgacagaag atatcttaat ccgattgaga
    10021 atgtaaaact ttacacaaca acccgcagcc aggcattaaa tctggggata tatacatgcg
    10081 atctgagttt tgccagtctt tatgaccaga cacagctgat tatagattac atgaatgctg
    10141 caaaaaaaat ggctgatgga cttggaattc ttgacgccat taaccaggaa actatttcaa
    10201 ggcttgagga aaatatcaat aacaaggaag tgattatgga aatcatttcc gaaacattta
    10261 tgaattccaa ttcatacctt gcagataatg accaggcagc agtagcagct attgtcctga
    10321 cgggaggatg gattgagggt ttatatattg caacacagct tgttgatctg aataatttca
    10381 atggcaacaa gcttgtggca accattatcg accagaaact ttcaatcgac attctcatca
    10441 acctgcttga gagcaataag ggcaatcctg ccgtggatga gcttataagc caggtaaaca
    10501 agctgaaagc agtatttgat aagattaata tacagacaac accaattaaa accgagattg
    10561 atcctgccac aaatgtgaca atactgaaat caaaggttac taccgatatg tcggttgaga
    10621 cttttaagga actaacagct gttgttaaag aaataagaga ttcatttgta aaataacacc
    10681 gccatgaaga cagtccgact tttttcaggt atattgctct tattaatagc tttaagtata
    10741 cctgcacatt cccagtgcaa ggctttcgca aaaaaagaag ttcttcctga actctcacca
    10801 tatgttcacg atggcaacta tcatgctgcc atacttgttg aaggtgaaga agctgaattg
    10861 tacaaaactt tttattccga catggaatac agggtcgtgg tcatgggcga aagcaaactg
    10921 ccggcagtgg agtttaaagt actggatgag aataaaaacg tcctctattc aagcaaggat
    10981 aatggtaatt ctagttcgtg ggattttaag cttgaatcga gtaagcagct gaagcttgta
    11041 ataaaggttt cttcttttaa taaacctggc gatgttcctg caagtggttg tgtggcaata
    11101 atgtttggct ttaaagtgaa aaaataatta agaattcaat acctgtaaaa taaagaaggg
    11161 cgttacacaa aacgcccttc tttattattt tgtttttgtc aatttattgc tgaggaattt
    11221 tccggtgtac gattttttac attttatcaa atcttcaggc ttgccttcaa atactatata
    11281 gccgcctttg tctcctcctt caggacccag gtcaattacc cagtcggcac attttatcac
    11341 ctcaggatta tgttctatga gaattaccga atgtccatgt tcaacaaggg cattcaatga
    11401 cccaagcagt ttgctgatat catgaaaatg aagtccggta gttggttcat caaaaatgaa
    11461 aagtatatgt ccgccagctt tttcctgact gagaaaatat gctaatttca cccgctggct
    11521 ttcccctccg gaaagtgtac tcgacgactg cccgagcttg acataaccaa gtccgacatc
    11581 aaccagaggt ttcagtttct ctacaatctt acgttcagtt tttccattat aactgctgaa
    11641 aaaacttatg gcttcatcta tagtcatttc aagaatatcg tagatattct ttccgtggta
    11701 ttttatttcg aggatttcct tcttgaatct cattccttta catgtttcac aaacaagctc
    11761 cacatcggcc atgaattgca tttcaacttt aattattcct tctcccaaac aatcctcgca
    11821 ccttccaccg tcaatattga aagaaaaatg tgacgctttg aaattattct gtattgaagc
    11881 ctgctgttcg gcaaagagtt tccttatttc atcataagct ttgagatagg tgaccgggtt
    11941 tgatctgctc gattttccta tagggttctg gtcaaccatt tcaacacctg tcaggctgtt
    12001 gatatctccc gatatttccc tgtaattacc aggccgtata cctgatcctt caattctctc
    12061 agcaagagct ctgtaaagta tatcgtgaac aaggctcgat ttaccactgc cgcttactcc
    12121 ggttacaaca gtaattgtat gcaggggaaa ttttacatca atcgatttga gattgttctg
    12181 ccttgctcct ttaacttcaa tataactgtt ccagggtctt cttaccggcg ggacaggtat
    12241 ctcgagctct ccttcaagat atttgatagt taatgagtta ttactttggg ttgaatcaat
    12301 tttcccctga aatatcacct cacctccaaa gagtcctgcc cctggtccca tgtctattat
    12361 ttcatcagca gcccttatta tttcttcctc gtgttcaacc acaagcacgc tgttacccat
    12421 atcacgaagt tctttgagca cactggtaag caggttaatg tcacggggat gaaggccaat
    12481 acttggttca tctaagatgt acagcgagcc agtaagatta cttcccaggg aagtggcgag
    12541 attaatcctc tgtgattcac cacctgaaag tgtcgaggag agtctgtcga gggtgagata
    12601 gggaagcccg acattcagca ggaactttaa tcttgaagta atttcatgaa gaagacgttc
    12661 tgctattttt tgttcctgct gtgtgagttt gagtgtatta agaaaattca gcaattcatc
    12721 tacaggcata gaaacaagtt cctgaatgga ttttcctcca attttcacat atgaggcttc
    12781 ctttctgagg cgtgtccctt tgcaggcagg gcaggttgtc ttacccctgt agcggctcag
    12841 taataccctg tattgaattt tgtatttctt ttcttcaagg tattcaaaga actggttgat
    12901 gccccaaaaa tagcgattac ctttccagag cacctccttc tgttcaggtg taagctggta
    12961 atatggtttg tgtaatggaa atccgaagag atgcgctgtg gagatgagac gctctttcca
    13021 ttttttcatt gactcgcctt tccagcatac tatagcgtca tcataaaccg acagattctg
    13081 atcggggata acaaggttct cgtcaatacc tattattttc ccgtatcctt cgcacaccgg
    13141 gcaggctcca atcgggttgt taaaactgaa taaatgctcc gaaggttcaa taaactgtat
    13201 cccgttctct tcgaatttgt cagaaaaatt ttccctcgtt attctgccac cttcatatat
    13261 tacaagctgg caaaaacctt ttccggtact gagtgcagtc tggatagaat ctgctgccct
    13321 gcttattccc tctgaggaat gggaaaccag taacctgtct attacaatat tgattttttc
    13381 cccggggttg cagcaaagtg gtgaatccgg atcatcaatc ctgattagat ccccgtcttt
    13441 ctcaattctg ttataaccct gttttacaag aaataagaag aactcttttg tagttatttt
    13501 tgaaggaatt tctgcgggag aagtgattat tacagttgta ttctcaggca gtgttttcag
    13561 aaaatccacg atatcgtcaa cactgtgctt cctgacttct ctgcccgtaa caggtgaata
    13621 ggttcttcct attcttgcat acaataacct caggtaatca tatatttcgg ttgatgtgcc
    13681 gactgtagag cggggattcc ttgtgtttac tttctgttcg atggcgatag cagggggtat
    13741 cccatctatg aaatcaactt caggtttttg catcctcccg agaaactgtc tggcatatgc
    13801 agagaggctt tcaacatacc gcctttgacc ttctgcgtaa atagtatcaa atgccagtga
    13861 ggattttcct gagcccgaga cgcccgttat cactaccagt ttatttctcg gaatacttag
    13921 acttatattt ttaaggttat gtacccttgc ccctctgatt tcaatgttcc cctccataac
    13981 aggtctgctt gtcaacactt tcagtacgtt cgtcaaaata atttgttaaa atccaaaagt
    14041 aatactttta cttttaatat tttttatttt atttgtattt agaactatca taaaaggagg
    14101 actgcctatt gaaaatctct actctttgaa gtcaaaagag tgagctatga aaaaagaaac
    14161 atctgactat gaactgttaa agaggttcat agatggcgag cagtcctgtt tcgaggaatt
    14221 aattcaccgc cacaaaaaca aggtatatgc gtatataagt ctttatatac gtgatcaggc
    14281 acttgctgaa gacatatttc aggatacatt tctgaaagta attcagtcgg ttaaagcagg
    14341 aaaatattat gaagacggaa aattcctgtc gtgggtgatg agaatagccc ataatcttat
    14401 tattgaccat ttccgccgca taaggcagat gaatacaatg tcgaacgacg attatgaatc
    14461 ggaccttttc aattcaaaat cgctggccga aaagaacgtt gaagaagata ttgtcagaat
    14521 tcaggtaagg aaagacgtca ggaaacttat aagcatgtta ccctatgatc agagggaggt
    14581 tgttatactt cgtcattata caggtttgag ttttaaagag atagctgaaa ttacaggcgt
    14641 gagtattaat actgcactcg ggcggatgag gtatgcgctt atcaatatga gaaaaatgat
    14701 aaatgagaaa aaaataagcc tgactgtaaa ataatattca ttacggcaaa tcgtttttta
    14761 tataaaaatg aaaacgtgtt tgcctatgaa tttaaactct actccttttg taaagtatga
    14821 tgaaattgat gttgaggatg ctgatctgat tgatgaggca tgggatttcc gggccgaatt
    14881 tcatgatgtg ttcgaattgc ttgatactct gaaagcagaa cctgacaggc aggttactga
    14941 aaggctcatg gaaattattg aaggtcagat gtgactttat acctccactg gagggatttt
    15001 taaaaaacac aggaaaattt tcctgtgttt tttgttttat taaaaatata tgatatattt
    15061 gtgatatcaa aatgatatca tcatgaaaaa agagtttgtc attaaaccca tgtcgggtta
    15121 catcgctctg ctggcagcat tattgtttct gggcggggca attgccggat ttatttatga
    15181 attaatctgg cctggaattg ttcttacggt actttttatt tttaccgtga ttgggttaac
    15241 aatagtttac ccgaatgaat catgtgttac agttctgttt ggggcatatc ggggcactat
    15301 aaaggagaat ggatttttct gggttaatcc tttttatctc aggcgcaaga tttcattgcg
    15361 tgcaaggaat tttgacagtg agcccatcaa ggtaaacgat aaaatcggaa atcccattaa
    15421 aatagggctg gttctggtat ggaaagtgga ggatacctat aaggcggcat ttgaagttga
    15481 tgattatcag cattttgtgc tggtacaaag tgatgctgca cttcgtaaac ttgcgggatt
    15541 gtatccttat gataattttg aagatcatga agctaccctt gcacttcgta gcggaggcga
    15601 agagattaac aacgagcttg agcgggaaat tagcgaaagg cttgcgatag caggaataaa
    15661 tgtgattgaa gcaagaatca attacattgc ctatgcagag gaaattgcca atgcaatgct
    15721 ccggaggcaa caggcaactg ctgttgtttc agcaaggtac aaaattgttg aaggagctgt
    15781 atcgatggtt cagatggctc tcgaacaact gtccgacaaa aaaattgttg agctcgatga
    15841 agagaaaaaa gctgcgatgg tcagtaatct gatggtggta ctttgtgccg acagggatgc
    15901 cacccctgta gtcaatacag gaacattgta tacctgattt atggtgaaaa agaaggcatt
    15961 tatactcagg attgatcctg aaaaactgga agcgctcgaa aaatgggctg ctgatgaatt
    16021 ccgtagtaca aacggacaga tagaatatat tatcgaccag gcacttcgta aagctggacg
    16081 attgaaatcg aaaccagtta gtgaaacaga aaaagataac aaagcaagca gttaatttct
    16141 ttaaagcttt cctgttactg gttcaggaaa taaatggaat ggcaggcagc aatgcctgcc
    16201 gtttcagttc ttaatcgact tgggccaaga tggacagatc tgaatcccat cttctctgca
    16261 gcccttattt catcatcagt aaaatctcct tcaggaccaa taagaaaaag tacgtcttct
    16321 cctttcttat atattccgga tacatgctct cttgtgaaat tggccgaaca atgtgctata
    16381 agctttatac cattaaaatc agcagaaata aaactgttaa aatcaatcga ctcattcagg
    16441 ttcgtcagat atgttttcag cgattgcttc atcgccgaaa ttattatttt ctttattctt
    16501 tctggtttta tgttcttctt ttcggtgttt ttgcatataa caggtgtgat ttcatccaca
    16561 ccaatctcaa cgcatttttc aaccagccag tcaaaccttt caggattttt cagtggagat
    16621 acagccagat gcagtctgta aggtcttggc tgaaaatctt ttatgacttt tgttattctg
    16681 acagtacacg cctcagggtc tggctctgaa ataactccct gataaaggtt tcctgtaccg
    16741 tcaatgagtt ttacaggatc gttttttttc agacgcagaa ctttcacaca atgcctggat
    16801 tctgtaacgt cgagaataca cttttcgcct ttgatgcctg aagcgtaaaa aatatgcatt
    16861 cctgaaagaa ttattatgtt cacttttgtt cttgttcttc agcctgatcc gaagtcatac
    16921 gctgcaaaaa tctcggcagc ctgtcttcaa cggctatcat gtatgtgtat ggcacccggg
    16981 ttgaggattc aggatgcgta cgaactttct ccttaagggt agagataaga gtattatatt
    17041 caagattgct tgactgtgtc atcagaacac ctctgaagta tgagaagtag taccaggttg
    17101 aattatctgc tttaagatat atatcgagca tatcaccaga tcttctcctc tgtatttcaa
    17161 taaatccgtc gacgtaaaca ttcacagcat ttgagcctat cagtccaatt ccaattttac
    17221 cctttgaacg gaatgaggaa gttggttcgt tccagtagag tttcacatcg ttcagcagca
    17281 gctcatatgg gtattccctc ggcaagtttc tggttgttcc gaacagacct atttcttcat
    17341 ttattcttgt tgctacagat tctcctaaaa ggtctttcat ggcttttttg tagaattctg
    17401 tattaagatt gaccggtgcc agtgctggta ccctccttat ctcattgctc atcatttcga
    17461 gtgcttgcgg ggagaaatag aaatcgagag caataacggc ttcaaagttc actttgcccg
    17521 aatcgatatt gtgaattacc ttgcctgcgg cagccatttt caggtggtca taatttgcgc
    17581 cgaaattaag ctgaccctca cccgagagaa tacaataatt tttatcaaga gtgatcatgt
    17641 tcccaggcag tgaaaggtcg gcaagttttt ccattgatgc aattctatat actccttttt
    17701 ccttctcata ccacaatgcg ccctgtgcgt tgactatctg ggcgtcgctc cagcttttcc
    17761 tctcagaaag aaatgccgga taaatatggg cggaatccgt tgatatgaat gagcccgaat
    17821 atactggatt gtcattaata tcccttacct tttcggctat agggataata accattttcg
    17881 ggtttatgct ggatttaaat tttacgttat agcttttgaa actgcagtta tgaacaattc
    17941 cggcagcccc ggtaaatgtt aggtaatcgt cctgcgccga gagcattaca tcgcctgtaa
    18001 aggtaaatgc agggctgagc ataaaattct gagtaacagg aatgtatcct gttgctgatg
    18061 taataccttt atcggatgtt attttcggga agttaatctg ctggatattg ccgctttcat
    18121 caacatagtt ataagttgca ctccctgagt agctgcgagt attttcaatg actatgtttc
    18181 ctgaatgaag caaatgcttg ttattgactg caataacagc attctgaaga ggttgaatcc
    18241 ttgctcccct ttcaattgtt attctgcctt tatcgggctg aataagagca tctgcgatgt
    18301 gtatgtaatt aatgttttca gcaataacaa cttcctgatt aagatcatat cttcctttcc
    18361 atgatgtgaa tgcgatagtg tcttttataa tattggttgc aaagaaggtg ggtttgtcaa
    18421 gcttattaaa gtcgagtttc agcagttctt ccggtgacat aagtggtgtt tcagctttac
    18481 ccctctgttc catgttaaga atcctgtttt tcatatcgta ctgaaaatct gtcattgtac
    18541 agatatattg tatttcgggg aactttacca gggatgtgcc tgtattaagg ctgaatttcg
    18601 actggtacga attgaagtcg atagttacat tgacattttc agcaagaaac ccgatggcct
    18661 caccccgggt tgattttagg ttatagtcag ctgtatcgct ttgaattatg ttttctgcaa
    18721 agttgaattt gtttgaaata attctggagt ctgtaatgtc aattattccc gaacccgata
    18781 atctttccgg ggtcagaata atttttccgt caagttgtgt gccattgcca aacatatcaa
    18841 actttttccc gggagcattg cttgcaatcc attcatcagt tgaggttaac catttaaccg
    18901 atacatcgtt ggatgagagc accggaaaac gaccgcttcc gtcaggtgat attgtaaacg
    18961 aacgggcaaa ggttatcatt gagtcgggaa agaacctgaa ttcatccgat tctgtttcag
    19021 aagtaatcct tcttagttta ccgctgccag tcagaccttt gttgctcatt gagatcatat
    19081 tgtataatct gcccttgcca tcatatactt caattccatc agagggaata ttcattgtaa
    19141 atccaagaga gttgtcttcc tgaattatca ggtattgttt cgttggtttt ataatgtttc
    19201 ccgcgtggaa ttctccaggg agattcatca tttcatttgt gtaatgatct atgttatcgt
    19261 aggtgaatgg ctcaatcctg aaataaaagt cctgttgttt gtaaattcct tcaagccctg
    19321 gtattttatc atagaaaata tatgaatagg tggtcgcatt gataatagga tactgtttaa
    19381 ggcttctcaa tcctgatttg tttttcgggt catctatata taattcagct gttgtaagct
    19441 gtatcaggtt ctcgacatcc ttaatcagtg gtcttcccat tgcatctctt tccccggttt
    19501 caacagctat tcttattgaa tcaattttgt gaaggttgat tttaaaagtg tcataatcga
    19561 atacaaaatt gtgtccgaaa attgtaaaaa gccctgcttc aacaactccg tcgaatgaaa
    19621 ttttcctgtt tttccctatt tcaattctct ggttgtacgg atagagagat accatttgtg
    19681 aatcgctcag aaaaatattt cttaccccgt ttactgtcag acggaagcta ttaaggtcga
    19741 gaaatgcatt ttctaccgga gcgtttgttt cgctcataaa agaaatcaca tcataatctt
    19801 tcttctttgc atatgcattt atgaagtcat ccactttgct ttttatagtc acttcattat
    19861 taaccctgtc gtagaaaaga aagcccctgt tggtaaggtc gatgcacagg ccggttacgg
    19921 cttcctcagg tttcttcatc cattttgcaa attcttttac aggaaacgtg tttgaataaa
    19981 accaatcggc aaactgcctc aggcggtaaa gtggatgata ttcgtcaatg ccagcaagtc
    20041 gttcgaaata tgttgaattg aaaaatgaag ctgactcgaa tcttgcctgg ccaagtgaag
    20101 ctccccttga gcgggaaaga attactgtcg attggttcat gttccagata aggttttcga
    20161 aatatatatc gaaaccatga aaggaatcaa accagggact ttttgctatt gggttgttac
    20221 ttctgaaaag gtttatttca cggttttcag cattatatga aaagcctatg cttgaatggt
    20281 gaatggaatc attccccaga taaaaagtag cagatgattc ctgagtgttt attcctccgc
    20341 gtgtaatgag acaagtgttg gcagcaacct tcaggtaaag ggtgtcattt ctgaaaattg
    20401 taatgcgtgc aggagtgaaa acatcaccca tgcctattac tgtagaacct tcaagcctta
    20461 tacccccttc ataatttaca tctttgaaaa tattttttat tgcaaaggtt ttggtgtatg
    20521 tttcaaacct gggatacctg atgttttcac gattacccga aagtgaaacc tggtcggtaa
    20581 gttttccgta ttcgggtttt tcaaagaaag ttttataggt aaatagtgca gaatcaacct
    20641 catatgcagc cctctgaaga ttaatagaat aattcctgat ttctgcaaat gcttcatttc
    20701 ggggataacc tgccttttca aaataaaccg taccaataac acccctgaac atggtatttt
    20761 caggaaaata ttttccggtt acgtcgtata tctctatact atctttctgg gaaaaggccg
    20821 ttagagttac attttgagca tttataaaga atgaagtatc gtattcaaat gttgtattct
    20881 gtccttttat cttccatttt accgttgctg attcgaaaac aatacctgat gttacgaacg
    20941 agctggtatt tctcaggtgt ctgtctatat tgtcgttgtt gatggctggt ttgtgtatca
    21001 tggctacaag tccagtaagc caggtgagaa aatagccttt gttgccttta tattgagaga
    21061 ggtcgagaag tgttttcaga aagtcgatga aatgaggaac aggcctgaag tttctggcag
    21121 ccagcaatga gctgatgccc ataatatttt cagcaatatc tgaggaaaaa tatgcgctgt
    21181 cccagctgga cgtaaattta ctcagaatgg cactctggtc tggtgtgagg ttcgggccca
    21241 tatatgcaac cagttctgcg gagaattttt cccgttcccc tgagaaaacc tttctgccct
    21301 gcgtaaaagc actacctgag gcaaagatga gtgccagaca cgtaataatc agctttttca
    21361 gcatcgtgca atcagaaaat tttacttcag aaacaataaa gttgataaaa tttttttaaa
    21421 aacggttatt tgtttaagct tattttggat ttcaattaga agctggacca aaaaagcgaa
    21481 aggggctgtg tacagccccc tttatagttg ataatctcct tattttactg cttccacaat
    21541 agcgcaaaat tcttcttttt tcagtgaagc gcctccaatc aaccctccgt caacatcggg
    21601 cattgagaat agctcagcag catttgatgc cttacagctg ccaccgtaca ggatggttgt
    21661 gttttcactg atacctttac catatttttc tgcaatcagt ttacggataa acgactgcat
    21721 ttcctgagcc tgttcaggcg ttgcgttcag gcctgttcct attgcccaga caggttcata
    21781 ggcaattaca acatttttaa attcatcttc agtcagatta aaaaggcctt cggtaagctg
    21841 ctcttttaca acatcaaagt gaatgccctg ttctctttct ttcagaattt caccgcagca
    21901 gtaaataact ttaagtccgg ttgaaagagc catcctcact tttttgttaa gcattttatt
    21961 atcctcttta aacaatgttc ttctttctga atggccaata attacatatt ctgcaccagt
    22021 gctttttatc ataccgcatg aaatttcccc ggtaaacgca ccagaaggtt ctgaatagca
    22081 gttttgtgca ccaaacttaa tattactgtc tttcagaaca tctttcatca cggaaagatg
    22141 aataaatgga gtgcagagta caatttctgc tttcggagtt tttccggcga aatattcttt
    22201 tactcctttt gcaagttcaa tgcctgaact tagatccatg ttcattttcc agtttcctgc
    22261 aacaattttc tttctcataa ttattaaata gtttagattc agttaattat ttgttttgat
    22321 aagattttca atatcttccg gaaggtttgc ccacgaccat tttcctgtta ttgtcccgtt
    22381 acgcagcatt atataacccg gattggaacg gaccattgtt ttaagagtta tttcgtccac
    22441 aaggcaaaac tgaaggttgc tatcgtaatc ttttatctta tcggatgaag aagcagtaag
    22501 aatatagaaa tcaataccat aggtcatgca tttgtggccg gtctgaaaac ctttattcag
    22561 tctctcaggt tttgcctttt cgagtttcgg ggagatcatt aaaagcgtga agttgctgtt
    22621 gttcaacacc atatctgtaa tatccctgtt gtctgcagtc actaacgaga aaccatgtat
    22681 tggaggttcg taacctttct taaccagaac agatttctgg tcaataaatt tccaggttgt
    22741 atcgtcagca ggaaaattat cgagggtaaa ttcttttctg atcccgtttt tttcgtaaat
    22801 gaaagtggtt ttgtaaaccg gggcaggagc accttcaggt atctccataa gctctttaat
    22861 gttgtttcct tttttataag gcaggaagtc tataatcggg aggtatttaa ggttataacc
    22921 tgtgaaaccc ataaagatga ttgatacaga gaatatgaca agccaacccg gtagacttct
    22981 aatgtattca agaggtttgt ttttatcgaa aattaaaata atcactaatg tcatcagcac
    23041 aacatttttc cagaaagtct gccagtttgt taattttatg gcgtcgccga aacaaccaca
    23101 gtccgacacg ggattggtta tagcaagtat taatgtgaga ggtgtaaaaa ttgacattag
    23161 tactgtagca cccatcacac ctgctttata ttttatacct gtcagcagtg agaagcctgt
    23221 aataaattct gccagacaga gcagaaaagc aagaggcagg cacaaaggct gcatgaaatc
    23281 aagtccgaaa gctgtgaagt aatcagagaa tttgtatgtt gaacccagag ggtcaattgc
    23341 tttaactgtt cctgagaaaa cgaaaactgc acctgtaatg attctagaaa ttactctcaa
    23401 aattttcata tcaggtcagg tttttttgtt caaggatgtt gatgattatc ctgaaaatta
    23461 tatcaggtgg taggattttt ccttcagtgt cgctgcagtt tacaacttca accaggggat
    23521 ctgtttcagc tactttcagg tacatctcac gcaccctcat ctggtatgca agactgcttt
    23581 catgaatgtc gtttttaccg ttgaggtatt ttctgtcgtc tccttttctt ctggttgaca
    23641 gcttactttc gacgaagttg aaaggcacat caaggaagag attaatatcg ggacgcggga
    23701 ttttgaaata ttcaaattca agttcaaaaa tccatttttt aagttcttcc tgtttttttt
    23761 catcatttag tttggcgcac tggtaggcta tattagaata tgtataacgg tcgaggagta
    23821 caactttccc ttcattaagc caggatttaa tcatcccggc tgcatctttt ctgtcgccag
    23881 catatagcat agcaaccaga tacgggtcta cttcgttgag tgaaccaaac tcacctctta
    23941 gaaaccttgc tatcagttca ccgaaccatg gtctatctgt tcgtggaaag tgcagaaact
    24001 cgcatttttt gtttcgggct tcatagaaat ttttcagaag tttaatctgg gtcgatttcc
    24061 ctgagccgtc gagaccttct ataacaatta gtttcatacg gaattcttta tgtgcaaatg
    24121 taataacgga gtgtgaattc agaaagaata tttgccactt atacatgatt attttatttt
    24181 tggattttaa taattatgct gaagaagtca gaatatataa ataccggggg taagcttatt
    24241 gatctgaaga ttccacggat tatgggcata ataaatgtaa cccctgactc attttataca
    24301 ggaagccgtt attttgatga taagagtatt ctggaagctg ccagcaggat gctggatgaa
    24361 ggtgctgatt ttatagatgt aggagggtat tcttcacgtc ccggtgctga ggatatcaca
    24421 cctgaagagg aaaaatcgag ggtgctcagg gccataaaac ttataacccg ggagattccc
    24481 gaagcaatag tttcagttga taccttcagg gccgaaatag catatgaggc cgttatggaa
    24541 tgtggtgcaa aaattataaa tgatatttca ggcggtgagg cagataaaga gatgttcaat
    24601 gtggtggcag aactcaatgt tccatatata cttatgcata tgcagggaac accccgtacc
    24661 atgcagataa atcctgcata taatgatgtt gtagcagaca tcctaatgtg gtttggggaa
    24721 agaatattta aactcaggac gatgggagta aaagatatta ttcttgaccc cggttttgga
    24781 tttggtaaga ctgttgaaca taactatgaa ttgcttcggc gtctgggaga atttaaaatt
    24841 gccgggttgc ccattatggc aggtctttca agaaaatcaa tgatctggaa aacacttgat
    24901 gttaaaccgg aagatgctct caacggtact actgccctta atatggcagc tctgatgaat
    24961 ggtgccgaca ttcttagagt tcatgacgta aaggaagctg tacaggtcag gactctttac
    25021 ctgaagcttc gtttaataaa taaagaaggt taataatatt ggacatgttg aataatttta
    25081 tggaaaaata attttgtaag tagtttttaa caatgccttt tcacattacc atatatgatg
    25141 taatcgatat tttccttgtg gccttccttc tgtaccagct atacagacta ataaaaggaa
    25201 caccggcatt aagcatattt gttgcaattt ttctgatata tcttttctgg cttcttgtaa
    25261 gagttcttaa gcttgaactt gttagtaccc ttttgggcca ggttataggt gtgggagtta
    25321 ttgcgctcat aattgttttc cagcaggaaa tcaggaagtt tcttatagtt atgggtagca
    25381 ggtatgttta taataataag cttttaggtc tgaagaagat atttcctgaa aacatggtgc
    25441 agaaagggca taagaatact gaagaaatca tcagggcagt tgaatacctt tcaaataaca
    25501 agataggggc tcttattgtt attggacgtt caggattgct tgaaccttat tctgagggag
    25561 gagagaagct tagtgctgcc attaatgcca gtcttctgga aacaatcttt acaaaaaata
    25621 ctcctttgca cgatggtgca gtgcttattg aaaatggaaa aataattgct gcccgttgcc
    25681 cattgcctat aaccgaacag gttaatttgc cggaagaatt cggaatgcgt cacagggcag
    25741 cactgggaat gtcggaacac actgatgcaa ttgtgatcgt ggtttctgaa gaaaccggga
    25801 aaataagcgt tgccgacagt ggtgtaatca ggagtaatat ttcaataaaa gatttgagac
    25861 aaattctggc aaacgagaga atataaacac agttagttac ttcatatact tcagtctcag
    25921 gttaacaatg cgctcgtaga caaaatagca gggattctcg cactgtgatt tttcatactt
    25981 tacccgggga tatgcagtat caactgctat ataatcttta ccatttatgt cagtgtaagt
    26041 tgcagttatt gtgtattttt tgttaagcgg cacgtcaatt tcccattttt cataatgtat
    26101 atagaaaaga ttaagaagaa cattatcttc aaggttaccc tgataaatcc ttacaattgc
    26161 tgccggatgc caatcggtta actgtttttc catatacagt accagggttg cgtggtcggg
    26221 ttcatcaggc atgcagtcgt tacaatcaat aaatatcggg ttctcatcgc atgagagaat
    26281 cgctgaaaat aacaacatta tgatatataa tgcatacttt ttcattctac cttcaatacc
    26341 atctgatata ttttcctgga gctcttattt tattgaaata tattgtgtat ccgattcctg
    26401 ctgttatcca tagaggtcca attttataaa aaggtgttct gaagtattcc agttcagctt
    26461 tcagcagaag gttatttttt gtccatttta atcctgctgc cggtaaaatt tttattttac
    26521 caccgggtgt ttcattggtt cctttcagtc tgttagcaaa aaagtaaccg cccgaaagcg
    26581 aagcagtaag ggtccagttc cctctaagag gataatcagt gagcctgaag tctttaaata
    26641 ggcctgcata aaacaggtag cttttatcca tatactgata ataaacgtcc ccttcttttt
    26701 ttattattac cctggtatat gcaggtttga aatcgattcc ggataaaact ccgaaatttc
    26761 tcaacggttc cttaaaagaa gctgatatgc cagtatagat atcgtggaga ttaaatttgg
    26821 tactggaaac aatatttacc tggtcaaatc ccttatggat agtctgagga atgttatatt
    26881 ttgttagcag atttataagt tctgttcttc cgtatgcgat agcaaccgca taagggctta
    26941 ctgcctttga ttgttctgat gatgatcatt tttctccttt tctgaggagg tattcaagag
    27001 cctctttatg gtttgacatt atgcagaggt caagggcatt gtaattgtaa ttgtttgttt
    27061 cgtatatatt tacaaatctt tttatcagca ggtccattat cagtgtatca ccattttgag
    27121 cagcaatgag aaatggagta aatccatcat tatcacgatc ggaagggtcg gcgccatttt
    27181 gcagaagaag atctgcaata tttgcatgtc ctgcccatac cgccgccatc agaggtgtag
    27241 taccttcatt atctttggtg taaattgcgg catcataata aagaagcata tcagcagcag
    27301 aaaagtatcc atatgcacag gcgtagtgaa ggggagtggc accaaacctg tctcttatat
    27361 tgacatctgc ccctgctctg gcaagaattt ctgcaatttc agtgttattc ttcttaacag
    27421 caatatgaag cggtgattct gagtaatttg aaagaacatc aagtttgggt ccatattcca
    27481 gtagaacctt taccgcttct gtcttgtcat ttcccacagc aaaatgaagc ggcgtcactc
    27541 cttccatagt ggatgtattt acatctgctc catactttat aagccatctg atgctgttaa
    27601 tttttccttc agctgctgcg gttaaaagtg catagttgag gtttaattct gcttctggac
    27661 ccctcagtag acttatatat ctaagagtat catcctcttc ttctaacctt ttattttcct
    27721 gtgagctgga aatatttgtg tgagaggcaa ttattaataa aactgccact attcgcagat
    27781 ataaatagag tttcagaggc atgatacaaa gttattattt tgattttatt ttgtatcgaa
    27841 aacttccacc atgttaataa ttatgatttt ataccgcaac aattatttct atttttgcga
    27901 agaaaatggt aaggaagtct cttaaaatat tatttatatt atcaattata ttgcttgcag
    27961 gataccaccc cgaagcagtt gaaaaaaata tctacctgaa agaaaaactt gtaccgggca
    28021 acataaggct taccaatgat ttttctgaag cacctgaatt cgaaaatttt gaacgagtta
    28081 ttcgttattt catgcgccgg tgggacctgg taggggtgtc ggtggctgtt gcaaaagacg
    28141 gcaaacttgt ttatgcaaag ggatttggcc ttgccgatag tacatcaaaa gaacaggttc
    28201 aaccatatta ccgcttcaga atagccagta tatccaagct gataactgct gtggcaatta
    28261 tgaaacttca ggaagagggc aagttgtcaa ttaatgacag agtttttgga cccgatggaa
    28321 ttcttaatga tccatattat agcaatccta aagataagag gatttacggc ataaccgtag
    28381 cccaccttct tgcccatgaa ggcggatgga caacccgttt cggggaccag atgtttatgc
    28441 cttttgtagt ggcaaaagaa atgggagttc ccgttcctgt tgatacaaag acaatagtac
    28501 ggtttgcact aaataagaat ctccatttta cacccggcac aggccgttca tactccaacc
    28561 tgggatatgc tatacttgga cttgtaattg aaaaagtttc aggcttgtca tacagtgaat
    28621 attgcaggaa aaaccttttt gaaccttacg gtatatacga tatgtatctg gcaaaaaatc
    28681 tgaaggacga gaagttgcct tttgaggtaa catattatgt gccacccgat gctatattga
    28741 aaccttctgt ttatggtaac ggtgaactgg tttctcccgc ctacgggggt aacgatatag
    28801 aagcactggg tgctgcagga gcctgggttg ccacagcacc tgacctcctg aaatttgttc
    28861 tgcatattga cggattaaaa ggcaggaaag atatattgca cgaagaatca atcaggttca
    28921 tgaccgacaa taacaataaa tatgctccca tcggttggaa gggtaccata aataacggag
    28981 tatggtggag gacaggctca ttcccgggta cagcaggtat ggtgaaacgc cagcctgacg
    29041 gtatctcatg ggtatttctt acaaacacaa gtacatggaa tggccctgag atatcaacgg
    29101 agataaacat atttatgacc agagcacttt cacaggtgag aaaatggcct gaacgcgacc
    29161 tgtttaatta ttatgtggag gctcctctgt catccgacat aacaatcgaa taaaaacgaa
    29221 cagtaatgtg ttttacagta aatgtaaaca ttgtaaagga agagctggaa gaaagatacc
    29281 atgcccggct gattgaccct gaaaactacc gcccaagcta ttattatcat gcattttcaa
    29341 ttccgaaact tcctgttgta cacaccggaa acagggaatt tatcgaacat atgaactggg
    29401 gcctgatccc ttcatgggta agagaccggg aaaaagctga tgaaattaga tataaaacgt
    29461 tcaacgcaag agcagaaagt attgatgcaa agccttcatt ttcaaattca tttgctacaa
    29521 ggaggtgcat tatacctgta aaaggatttt ttgaatggca gcatgttggt gaaagaaaaa
    29581 ttccatggta tgtcttcaga gcagatgaag atataatgtc gcttgcaggt ttatggtcgg
    29641 aatgggtgga taatactacc ggggaagttg taaaaacttt tactgttatt acaacagagg
    29701 caaatgagat gatgtcaaag attcacaact ccaaaaaaag gatgccggtt gttctggaaa
    29761 gatcatcaga agaaatatgg ctcgatttga aagttccaaa agacaagctt ctgaaactgc
    29821 ttgaacccta tcccgataaa attttgaagg cttatacaat aggcacactt attaacagaa
    29881 aagatgcaga taaaaatagt gccgatttaa ttaagcctgt tgattataac ttaccgggaa
    29941 aactgctttg ataatttcca gttagatcct gttaccactg gggaacaaaa tggtaggtga
    30001 gggtaccagt ctggttcttt gaggctgttg catcgggatt aaatctggag gagtacgccg
    30061 aaatgatggc tgcttctgtc aggcaaggat cagaggtagt actttcacct gcaattattg
    30121 ctgcttttac tacttcacct ttcggattga cttcaattgc cagcacaatt ttccctccgc
    30181 cttcacactt atagatagga agtggcagat agacgtgggt tcttccgggc agatagtaat
    30241 atatccgggt aggaccactg tatcttgccg ccatttcgtt agactcacgc attttttcat
    30301 ccctgtcttc ttcatctttt tgaacttcaa tacccctgtc agaggattct gccttcagtc
    30361 ttttttgttc atcaatatag ttatcaggac caagaagacc actttttatc atttcctcct
    30421 tcactctgtc gatgtaatct tcaggattaa ttttaaaatc gggctttgcc gcaagatttc
    30481 tcgcaatatt cagtatttcc tggtcaacag cctgattatc atcattaata ccaacagatt
    30541 gtttcggtgc aaattcttct ggcagattct cttcaagaaa aataatttca tactcccgca
    30601 tatatttgtc aatatcgagg gaacctattt taacaatcat aaaaatgata cccgccgtaa
    30661 ggtgaataat cacagctccg agaattccaa caagtttttc ccttccgttc ataattctgt
    30721 gcgaaattat aaattattac ttttgccact cattccatag attaattata taacaaatgc
    30781 cagtagaaat aattgttaat cagattctga tacttgctat agttgcactg gttggtgtaa
    30841 ttgcctcaaa agcaggagta ataacttcag aagggaaaga ttttcttgca aaaattatat
    30901 tcaatgttac ccttccttcc atgcttctgg caagttttgg cagtattgat gctactccgc
    30961 gactgcttac aaacagcctg gagtttattg gactctcctt tttctcaatt atgctgttgt
    31021 ttattgccgg aacggccgca tcatccatac tgaaactcaa aggtgaagag tcggcaattt
    31081 tcagaattca cagcatgatg ggcaatatta tataccttgc atttcctgtc atttattccc
    31141 tttatggaca ggaaggcatt ctttacggaa gtatttttac aatagtttca aatgtagttt
    31201 tatggacatt aggaattgtg ataattgccg gaaattccaa aatatcagta aaagaaagct
    31261 ttaaatatat catcaaccct aacacaatag ctattctgac aggattttgc cttttcctgc
    31321 tgcatattaa gttgcccaag attattatag attcagtagg aggcatcgga agtacaaata
    31381 cctatctttc aatattatac ataggttcgg ttttgtttta ttcaaaaacc gggagatatt
    31441 tgaaaaatat aaatatttat gtcctgtcgt ttaacaaaat gatccttgtc ccttttctgc
    31501 ttacaggaat tttttggtta gcagcggctt atcttaacct gcattttgac aaactggttc
    31561 tctcggtatt aatacttgaa tcagccatgc catgtatggt gaatgttgtt attatagcaa
    31621 aaatcctcgg aatggatgat gcacctgccg ttgccaatgt attcgtaagc acaatcttaa
    31681 gtataattac tttgcccatt gtattgatat ttctttctgt tttaaagtaa tttttaaaat
    31741 ctaacctatg ggaaaattat ctgtattaga agtcattaga gatagatgga gtccttactc
    31801 tttttcatct atccgggttg aagaagaaaa attcgaactg ctctttgaag ctgcccgttt
    31861 tgcaccttca tcaatgaatg aacaaccctg gctttttata tttacaacaa aagatgatgg
    31921 gaaatatttt gaaatttttc ttgattttct ttatgaagga aacaggatat gggctaaaaa
    31981 tgcttatgtt attggtattg taatggcaaa aaccagattt tcaaaaaacg actctccaaa
    32041 cagacattca ctttatgata caggtatggc tgtgggtaat cttcttgcac aggcaacttc
    32101 catgggtttg tatgttcacc agatgggcgg tttttcggtg gataaggtaa agaaacattt
    32161 cggggccgat gattatattg aacctgtagc gatgatggcg atagggtacc tgggagatgg
    32221 aaccgacctg agtgatgagc tgaaagtcag ggatatgaaa agaaggccgc gaaaagcatt
    32281 gaacgaattt gtattcagaa atgaactgag taacccggct ttcaagtaaa tcagaaataa
    32341 gccgtgaatt ttaagttaag acttcttcct gtaaggtaat cgggtattgc gaattgcctt
    32401 gagcggccac tctggttatt gacagtcttt atccagaaat atgaaacagt attgtttata
    32461 tcaagcaggt tgaaaatctc tacacataca atcaattctt ctatgccact catgaaaccg
    32521 gatgacgaaa agttattgaa gacttttgtc atcccgaggt caacgcgccg gtatggaggc
    32581 aacctgtgat actggtcgta ccggctgtta aatggagata ttaccggcag tcctgtcaca
    32641 aacgaaatgt taatatgtgc cctgtaactc ggatttccgg gtaaataatc ctggaaaaat
    32701 atattgcagt tgagtgtctg ttccgaaggt gatggaaaca tatcctcgat aataccaggc
    32761 atttctagtt tcgaatccat aatagaaagg ctgaaccacg attcggcgcc ttcgacaaat
    32821 tcaccattca gtctgaagtc aatacccctg ctgtaacctg tagccatgtt atctccttcg
    32881 tatattattc ttacattatc aagacggtaa gggataatac cagaaagctt tttgttgtaa
    32941 atctctgctg tgaatctgaa tggtctttcc catgctttga agtcgtacga catgcctgtt
    33001 actacatgaa atgatttctg ggacttgata tttttattaa gtgttccgtc agggaagcgc
    33061 atctctctgt aaaagggcgg ctgaatatat gctcctgatg ccagccacgc cgagatgccc
    33121 ttttccattt caactctggc agaaaacctt ggacaggcaa aaaactctga tgtaaatgag
    33181 ttatatgtgc ttcttatccc tcctgttagt attgtattgt atttttctcc ccttaatgtt
    33241 gcccttgctt ctccatatgc ttcgtaaata cggttgatta ttctgttgct ccctttcaac
    33301 attgaactta ctctgagttc gccgtttgta ttccatggaa ttgaaaagcc tgctgagtca
    33361 accattttcc attcacggat attatcgtta atgatttcat tgtttgcctt cagaccccat
    33421 ctgaaagaca ggttatctga agcgttcata aaaccgctgt actttatcgt aaaaaatctt
    33481 gcactgattt tgttccgtgc atgcgaaagg aaacttccta ctcctatgtt taacagagta
    33541 tcaaccatgt tttcagaacc catattcctg tccagcatgt ttagactgta aaatcccctg
    33601 atgtcgaatt gttccctttc gtttgttccg aaatttgaaa taattatctt atgtgttgat
    33661 tttattccag aatatttcca ggttaatgca gtgttccagg tctggtacct gtctttttct
    33721 cttccttcga agacaacata gagcctatag gcttcagttt catttccaaa cttgctttcc
    33781 ctgctgcgcg gtacataatt ataggtattg gatgaataag tgccaaacag tgaaatctcc
    33841 gacttatttc cagtcttaat ggttacaatg ctctgtatat ctgcaaaaac tggcaggtaa
    33901 tcgccttttg aatcaagggt ttttagcata agcctgctcg atttgtacct tagtccggta
    33961 agccaggtta ttttacccga gcgacttatc ccttcaaaat gtgcagtgtt aatcaataat
    34021 cctgcagaaa ctgaaccttt taaattatca gggcgcctgt aagtaatatc gagtaccgat
    34081 gacattttat cgccgtatgt gacgccgaaa ccacctgctg aaaacaacac tgagctcacc
    34141 atatctgggt ttataacgct aagaccttcc tgttgacctg atcttatcag aaaaggcctg
    34201 aatatttcaa cgtcattgat ataaaccagg ttttcatcga agtttccgcc tcttaccgaa
    34261 tactggctgc tgagttcgtt atttgaggaa acacccggaa gagttttcag tacagtctca
    34321 aaactgcccg atgctgaagg catcatgctg aaatctttcg gagaaatact gatcattcct
    34381 gctccagact gcttgctccg ggctgataca gtaatttcat caagtcctgt aacctttctg
    34441 ctgaggatta tttctcccag atcaatatta tagttacaaa cgattgttat cgcttttctt
    34501 tcataacctg tgacagaaat actgagaata tgctcttttc catctttcgg aatagaaagg
    34561 gagaataaac catgattatt ggtatatatg cctgtattat agttgcttat agttatggtt
    34621 gcattttcaa ctggaattcc tgttgtatct tttacaacac ctctgataac aattagatcc
    34681 tgggcagttg aataactatt actcagaaag actaaaggaa taacagcctg gattattatt
    34741 ttgggtaacc ggaccattta tttctgtcgg cgtgcttttg ttttgctgcc tgacttctct
    34801 tctgattttt catttttaag aagtccgctt ttcagtccgc tgaagaattt tgcccatgca
    34861 aaaggattga gcaggttgtt agctggattc tgattgcgtg tcataatggc attggcattc
    34921 tgctgcattg ccatcctgaa tccggcttcg ggcgtaactg catacgctcc ggtctggttt
    34981 atgctctcct gtataagata gagattttca tacatgtatt gaatctccgg cttgataaca
    35041 ggccgggtgg caagaaattc ccttttgaat tcctcatagg ttttccatgg aaatattgtt
    35101 atgccttcaa ttgaaatggt atcggcaaca agcattacat cctgcttgta gatacggcca
    35161 tcaaaatcgg aagggatact gtaagaatat tttttataac ctagtgcact gatgattatt
    35221 gtatctccgg gcatggatat aatggaataa atacctgtta cttcacttat tgtacccctc
    35281 cgaagttttt gggaaaatac cgaggcattg tatacaggct cgttctcacg gttagtaatt
    35341 accccgcttg cctgtataag tttatacttt tgactttcct gagcctctaa tactctcagg
    35401 gataccacca acaaaaaaaa tatataaaat acccttctca atagttgttt ttatttataa
    35461 cgcaatccga caaaaaagag tctgtaaagt aaataataat gattttattt ggtaaaactt
    35521 ttaacttaat tttaataaat gaattgttct ggttaaacaa tttcttgttt gctgttcctg
    35581 ataaaaatcg ttattgagtt gatttaattt tgttaatatt accatgttat aaaaatcctc
    35641 tattatgcag agattattgt tgcttggaga tgaggcaatt gctcagggcg ctattgatgc
    35701 aggcataagc ggaatgtatg catatcctgg aacgccgtcg accgaaataa tggaatatat
    35761 tcaggcttct cccgaaggaa agtcaggcag gatagaatgc agatggtcgt cgaatgaaaa
    35821 gactgcagtt gaaacggcac ttggtatgtc atattgtggg aagagagcca tggcagccat
    35881 gaaacatgtt ggactgaatg tcgcggccga tgctttcata aattccgcca ttacaggggt
    35941 taatggcggg cttattattg ttgcggcaga tgacccttcc atgcattcat cacagaatga
    36001 gcaggattct cgattctacg ggaaatttgc gcatgttccg gtatatgaac ccagaaatca
    36061 gcaggaagct tatgatatgg tatttgatgc ttttgaacta tccgaaaagt actcgttacc
    36121 cgtgttaatg agaatcccaa cccgacttgc tcattcaaga tccggtgttg taaaacggag
    36181 acagattcct gaaaaaaaat tcaggcttcc cgatgattcc aggaggttta ttcttatgcc
    36241 ggcaatagca cggaaacgat tcaaaatcct gctggaaaat tatgaaaaat tgcagtttga
    36301 taacaggctt acaaaatata atacttttaa agatggaccc gaccgaagcc ttggaattat
    36361 agcttgtggg attgcagtaa attatttgat ggagaatttt tctgatgatg aattgccata
    36421 tccgttactt tcggtttcaa cttatccttt ttcaaaggat atcataaagg acattaccgg
    36481 taggtgcagg gagatacttg ttcttgaaga gggctatccc ctggtagaag agcagcttag
    36541 aggcattctt gacgaaggca gaaggatata tggcagaatg gatgggacaa taccacgtga
    36601 cggtgaactg aatcccgaca ttgttgcatc ggctcttggc attaaagctg ggaaagggca
    36661 tactgttcct gaaatagtca aaagcagacc tccttcattc tgcaagggat gcgggcatgc
    36721 cgacatgttc aatgcaatag ttgaagcaat caaaccctac gggattcagg gggtcttttc
    36781 tgatatagga tgttatacac ttgcagccct tccgccatac agctgtataa attcatgtgt
    36841 cgacatgggc gcttctgtta ccatggcaat cggtgctgca gatgcaggtc ttttcccctc
    36901 catagccgtt ataggcgact ccacatttac ccattcaggg atgcccggac tcctggatgc
    36961 agtggcaaaa aattcaccgg ttacaattat tatagccgat aacctcacaa caggcatgac
    37021 cggcggacag gaatcgaatg ctatagggag acttcagagc atatgtactg gtattggagt
    37081 tgctccggaa catgtgcgaa caatcgttcc cctcaggaga aatcatgaag aaaatgtacg
    37141 tataatgagg gaagagtttg aatacagagg accttcggtt gtaattgcaa caagggaatg
    37201 cattcaggca ggagccagaa ggagaaaaag tgaaaaatct gaaaaacaga aaaacaacga
    37261 agaatgaaaa aagatataat aatagcaggc gtaggcggac agggaatact ttccatcgct
    37321 gcaacaattg gtcttgcagc agtagaaaaa aacatgtttc tcaagcaatc ggaagtccat
    37381 ggcatgagcc agagaggcgg ggatgtggtt tcacatctga gaatatctga taagcccatt
    37441 cattccgacc tgatacctta tggcacttct gacttgatta tatcagttga gcctatggaa
    37501 gcactcaggc atttaccatg gcttgctcca ggtggatgga ttgtgacaaa ttcaaatcca
    37561 tatgttaata tatccgatta tcctcctgta gatgagataa ttaagaatat aaagaaggtt
    37621 aaaaacagca taattgttga tgctgattca attgctgctg attgtggttc cattaaagct
    37681 gcaaatatgg taattcttgg agcagcatct ccttttatgg gaataccgct ggaaaatctt
    37741 gaagatgcat tgaaaaaaat gttttcaaaa aagggagagc agatagtgga aatcaacctg
    37801 aaggctttca ggtcaggtag aaaaattgca gaagttcaat gaatcagtac ctgtagtgca
    37861 ggtgaataaa aaacatgtca gatgcgcttt cagaagggtc tgcaggaatt tttccttgat
    37921 aatcaattgc aatacgaaca ccaggaatga aagtatgttg caatccgaaa actataagct
    37981 ggtggtcatt tttataattc cattcaatga tttcaccagg tagaacaaca gaagaagaat
    38041 aatcatatct gccaaaaagc tgaagtttat cagtcaggtt tacagaacca gtagccgaaa
    38101 aaccccagac attatgccct ctggtattat cgtgacttgt tcggtaattg aattcacctc
    38161 caatcttaaa aacattccat gtaaagcctc caaaaccgat aaaggttgat tgccatattc
    38221 cggcaatttt tcttgtatca cagtaaactc tcagaatagc atcatttgca ggaaagtaat
    38281 tgaatccaaa tgaagctttg agagacccat ggtcgttaat agctacacct ccgccattca
    38341 tcagggtgaa atcgatgcct gtacggttgc tgatattata atctattgca attccaagat
    38401 catataaact gccatattta tttatcgact gaaacgtgtt ggcaacatat cttttgtcat
    38461 agaaacgaat ctggtaaatc atgattctgg taccagttac acccatcctg atctttaatt
    38521 ttccgttatc atatccgatt gatacctccc gtatggggga attgtttcct tcatggggga
    38581 ttgaatcgaa tagttttgcc atgttaagta ttatggagcc tgaaaatcga ccctctttca
    38641 ggaaatcacc cccaaaataa gctctcgtga tactgaattc aggtttatga gtattgtcgg
    38701 taatcgaaaa atgaaaatct gtaaaaattt ctgcagaagg tgtaaatatc ttttcttcct
    38761 gttgtgaaaa tatctgaaat tcagctattg tcaataaaat agttaatata aaaatttgtt
    38821 taaagtgtga catgatttga ataaatggca gccaaaatta ttcaattctg aataatatca
    38881 atatatttgt ccggccaatt gatgaataaa attatgaaaa aagttgtgtt aagtatttta
    38941 actcttttag caataggcat ttatactatt cagatggctg aagcacagag caatgtaaag
    39001 ctttacgacc catcacttga tggtatgaaa cagatcaggg aggctgtggc tcaggcaaag
    39061 aaatcaggaa agcatgtttt tattcagtac ggcggcaact ggtgcccgtg gtgcattaaa
    39121 ttcgatgcat tcgtaaaatc cgatacaggg ctaacacgta ttattaatca acattatgtg
    39181 cctgtaaagc ttaattatga tcagacaaac cggaattatg aagcaaacga atatctggga
    39241 aatcctattc gtttcggttt cccggtattt attattctta atggtgacgg gaaagtactg
    39301 cacattcagg acagtgcatt gttggaagag ggagaaggtt ataaccgcca gaaagtttac
    39361 agtttcctta acaactggac tccatctgca ataatacctg tgagaaaaca atagctgtta
    39421 tataaataac agaaaaaagg gattgttatg tacaatccct tttttctgag gaaatgcagc
    39481 gcttcaccta agctttttta ttctgctgcc tgtttcaccg gtgagcctgc agggctgaga
    39541 cacagtaagt tgcttgcgac aatctcggtg gtgtattttg taacaccagt tttatcttca
    39601 taactgcggt atgcaatcct tccttcaaca tatacctgcc ggcccttttt aaggtacttt
    39661 gaggcaaaat cggaaagtct gttccatact acaatgttat gccaggtcgt ttcggttacc
    39721 ttctgtcctt cactgttttt gaacgtgtca ctggtggcaa gagtaaacct tgagactctt
    39781 ttcccgctat caagggttct ggtttccgga tcgtggccaa gattgccgat caaaaggact
    39841 ctgttcttca tctggttcat gactttaaaa tttaaggtta aacaataaat tatttcacag
    39901 caaacataaa ccaggtaaat aaggtgtctc ggttgataaa tatttaaatt cgtttaaaag
    39961 atatttataa tcgtttctat tcgtttgtaa acgttttttt atatctttat caaaacttta
    40021 tcgctgtgga aagaagatgt ctcgactgcg gtgacgttat tcacggacgt tccgataaaa
    40081 aattttgttc cgaccagtgc aggaacaatt ataataacaa gttaaaccgc gacaccaaca
    40141 attacgtaag gaatgttcat ggcctgctga ggaaaaacag gcggattctc tgcgatcttt
    40201 acaatgaagg taaaagggtt atccacaggg atgccctgtt tgcccttggg tataatttca
    40261 atttttttac ccatattatt caaacccgcc agggtgataa gtaccactat tgctttgaat
    40321 acggttacag ggaaaaggat gatgagtata ttgagctaac tcagaatctc cagtatctgg
    40381 agtatatctg atcttatctt gtcttttatt ttcggattga gtaagaaaca ttttctggtg
    40441 aaagaggtac tagaatcccc ctgtaactta ccgactgtat gtacggattg ttaacagaag
    40501 cggagcaata accattttca cccagagtaa ggtatatatc aaatttgtcc ttgtttggag
    40561 taatagcctc aacctgtata ttataaatgc ctttcgcttc atcgcttttc attaaatatc
    40621 ttcctgttct gccattgaaa tttattcccg atatttgtct tgcaccaaat gaacgccctg
    40681 cataacctaa acttacagaa attatttcac cattaattat tataaagtta tttctcggaa
    40741 caagatctac catcagtccg tcggggagaa ttattttatc cattttaagg acaaagcgcc
    40801 tcgattcaat tgcttttttt atagccacct gtgctgcaag cctctctgcc tttctctttt
    40861 cagccctcag ggaatatgat tcttttgata ccgtacatga ggaggcacca ataacaagaa
    40921 caatcagcaa caaaaatatc ctactgttca taaatatctc tttttctatg atacttcaaa
    40981 attcatgccg ctaattactt tcatgagtta aattaatgtc tttatttacg ctaccatggt
    41041 tctaatgttg ttaaggtaat tttaaaaaca cctgtgtttc gcgagcagct ttatgattaa
    41101 catgatgaaa aataaccatt aaaataactt taacacagac aatgaaatat gttaaaaatg
    41161 acatttttag agataatgag gtaaaaaatc gtgttctata tgcaaccttt taccccatat
    41221 ttacgttatt aatgtgttga tatatctaat ggagtctaac ttaaattttg aattatgaaa
    41281 aaactatttg ttttattgtt tgtatcattg atggcaattc cggctttttc ccagattaaa
    41341 ttcggtatta aggccggagc tgcaacagca acagtgccaa aatatgatct ttcaacaggt
    41401 acaacaaata ttgaggcagt gaaagatgct gcatggggtt tccacggagg tttgtttctg
    41461 aggttgaccc ttcttggtat aaatattcag cctgaggcat atcttgcaac caacacttat
    41521 gaatataaag taacttcagg tactttacca gagattaaaa agcagacttt caatagactc
    41581 gatattcctg ttctggttgg gtttaaactg ggccctgtgc ggcttaatgc aggcccggct
    41641 gctgctatac agataggcac tccgaaagcc cttatcgacg atccaaattt cgaagaaatg
    41701 taccgtggag caacctttgg ctatcaggca ggtttgggaa tagacctgtt caagaaaata
    41761 acactcgatg ccagatatga aggtagcctc agcggtaagt tcggtgaagc tgttacaatt
    41821 ggtggacaaa ccttcaaact cgaccagcgt cagccagctt tcgttctgag cgtgggcctt
    41881 attttctgaa cgaacatcaa ttatattcga aaaactctcc ctcattggga gagttttttt
    41941 atttttacac agcctattaa cgaagtatat gacagaagta gccgaaatgg gttttccctg
    42001 gtttcagttt ttgataatat cattttacct ggttacggcc ctttttatat gcttcatgat
    42061 tatttttgaa aacagggctc cggtaaagac tctttcatgg gtgatggttg tgcttctggt
    42121 tccggtagct ggaatcattt tttatctgct cttcgagcaa aattaccgga aacagaaaat
    42181 attttcgaga aagagtgtcc tcgacatgga acaacttagc aatatagctg ctttccaggt
    42241 aaaaactctg ccagaacggc ttaaaacggc aagcgaaaaa gtgcgtgaaa aggaacattt
    42301 gattaagctg atgctaaata acagcaagtc agttttaaca gagttcaata aagtagatat
    42361 ccttattgat ggcatcgaga ccttcccgtc aatgttcaca gcaataaatg aggctaaatc
    42421 tttcattaac attgagtttt acaggtttga atctgatatt cttggcatga aattctgcga
    42481 tgcactcatt aataaagcaa aggaaggagt aaaggttagg gtaatatatg atgatgtagg
    42541 aagctggaat ttgagtaaaa aagtagtaaa aagaatgcgg gaaagcggag tcgagatttt
    42601 tccatttatg cctgtcaggt ttccatggct tacaaataaa ataaattacc gcaatcacag
    42661 aaaaatactc gttgttgacg gaataaaagg ctatgtgggc ggtttaaaca ttgccgataa
    42721 atatcttcat ggtcatcgtg aaataggtaa ctggcgcgat acccacctgc ttataagagg
    42781 ggaagccgta acagcactca actatgtatt tatggtcgac tggtattttg ttagcgacat
    42841 actgcttact gatgaggaac ccattgatga taaatacaag gttgctgaca ggtgctggat
    42901 tcaggtagca tcaagcgggc ccgattctga ctgggcaaac atcatgcagg tttacttctc
    42961 ggcaattgca acggcaagaa aatacatata cctctgcacc ccatattttt cacccgacga
    43021 aacaattctc aacgccctgc ttacagcatc cctttcgggt gttgatgtca gacttattct
    43081 acccggaaaa tctgattcgc cagtttctta ctggaataca agatcatata tcagcgaaat
    43141 gatggatgca ggtataaagg tttatctgta taacgacgga tttaaccatt caaaatacct
    43201 gcttgttgat gacgtctttg cttctgtagg ctcagcaaac gtagatatga gaagctttga
    43261 ccttaacttt gaaattgcag caatgatcta tgatgaagat tttgcagcaa ggcttttgaa
    43321 ggtattttac atcgatgtgg caagaagcat tgaggtcaaa cccgatgagt ggaacaaacg
    43381 aaagaaaaca gaaagataca aggaatcaat agcacgcatt tttggtccgc tgtattaatc
    43441 aaaattttta tgcatgctga aagagggaga agaatatatt tttacagctg gtaaagaaat
    43501 cgaaacaccc gacgggaaat tatacaggat actgcaaggt cctgataatg ttaaatacct
    43561 cctcagggct gattattata aagaatatga aataacggaa ggaaaaagaa taacctgtcg
    43621 ggtcgacaaa atcaactgca ggggagaagt ctttctcgaa ccccgccatc cggtttatga
    43681 accaggtaaa caatataaat ttaaggtagt atcacatgaa tttcgtagcg acgactctgg
    43741 caatgataca gccgtgatcc ttgtcgaaga tgtcttcgga aataaaattg ccctgccatc
    43801 aggagttcaa aatactgaat taccggcatc aggctcagag attcttctta cagtcgaaag
    43861 aatcttcaga ggcaggccgg ttctctacag accttcttcc gaaaaaggaa ggttcaaagg
    43921 tcttaaatac ggagaatatt atgacttcag aatagaaaag gtatcaaaag gcatagacgg
    43981 agaagaaatt ttcgttgttt cagacccgtt tggaagagtt catacaattc cagttaaata
    44041 tttcagccat tacaacataa atcccgggtc ggtatttaaa ggacgaatca taaaacattc
    44101 aagaaatagt tcatggaaaa ttgaaccccg caatccgtat tacaataaag atgatataat
    44161 aagccttaaa gttgaatcag ttatgcccgg tgtaaacgaa aattctgtca ttctaacact
    44221 gaaagatgaa tttggatttg ttcatctggt aaattcgaaa accaaacctg aaaataaact
    44281 tgtaaaatgc agggttaaaa ccatcagaaa ag
//
LOCUS       DAOSNB010000002        29176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_1610_length_29176_cov_4.511624, whole genome shotgun sequence.
ACCESSION   DAOSNB010000002 DAOSNB010000000
VERSION     DAOSNB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29176
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_29176_cov_4.511624"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..2965
                     /locus_tag="PLG42_00215"
     CDS             320..2965
                     /locus_tag="PLG42_00215"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU29662.1"
                     /translation="MKRVILSGFFIYLSVSFLIGQNETADIKKQYNAVYITTAPVIDG
                     KLNEDVWGQGNWNDDFIQFEPLNGRPATQKTEFKILFDEDNLYIGIKAFDTSPDSIVT
                     RLTRRDQADGDLVGVIIDSYHDLRTGFLFGVSASGVKFDQIFTNDGQNQDSSWDPNWW
                     VKTSVNDEGWVAEMKIPFSQLRFEKNSGDVWGLQVARIYYRKNETDFWQHIPREAPGM
                     IHLLGEMTGLEKIKPRKIFDVTPYAVAKAETFKAEPENPFLKSGKRFGFNGGIDAKIG
                     VTNNLTMDLTINPDFGQVEADPSEVNLTAYETFFREKRPFFIEGNNITNFGLGLGDGD
                     IGNDNLFYSRRIGRRPQGFPSLNEGWHADIPTATTILGAAKLTGKTQKGLSVGFIEAV
                     TAEEKAEIDTVGGKKYETVEPLTNYFVGRVQKDYNEGNTIIGGIFTSTNRRLDDNLAS
                     FMHREAYTGGVDFTQYFKKKSWMYNINTAFSYVAGTKEAIELTQRSSARYYQRPDNDY
                     VNFDPDRTSLFGSGGRMQIGKMDGHLNLMGAVIWKTPGFETNDLGYIREADQILSVLW
                     AGYSQWEPKGIYRNYRFGGDFYFVNNFGGDWLGKGFEWFANMNFKNYWFAWTGGNINT
                     TQLSTGMLRGGPMIKLPGNINMRLGFSTDNRKKLVLQVSANNSTGLEGNSSSWYTMAD
                     ISFKPTNYLRVSFNPSYNPSFTELQYVTKKSFGNDTRYLFARIDRKTLSASLRVDFNL
                     TPELTLQYWGQPFIATGKYSEFKYITNPMADEYKDRFHVYTPAQISLNSGSYDIDENS
                     DGTTDYNFGKPDFNVQEFLSNLVIRWEYNPGSTIYLVWSQTRSCFNDSGQMDVFNDLG
                     DLFNCKDNKPHNVFLVKFSYRFGLR"
     gene            complement(3089..4189)
                     /locus_tag="PLG42_00220"
     CDS             complement(3089..4189)
                     /locus_tag="PLG42_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOU29663.1"
                     /translation="MRQVFTILLTFCIPAIYSYSQEALDTLVPVRGFCIAAPRKAELD
                     DFITFIKEELVPRKVNTLILRVDYNYEYKSHPELRDSIALSKRDVKKLVEVCRDNKIT
                     IIPQINLLGHQSWANRTGNLLRVYPQFDETPWVKMPEKYSWPNPDRLYCKSYCPLHPE
                     VHKIVFELVDEICDVFEAGAFHAGMDEVFYIGEDQCPRCGGRDKAELFAGEVTLIRNH
                     LAQKGRRLWIWGDRLIDGKTTGIGEWEASFNNTHRAIDMIPKDVVICDWHYESPLPTA
                     VYFAMKGFDVVSCPWRKPEVGVAQINNIILFRNSSTPEMKEHFKGVVQTVWSGVKQFL
                     DSYYGKSTEASENTAANCFRDMFNEMSRLLNK"
     gene            complement(4233..4673)
                     /locus_tag="PLG42_00225"
     CDS             complement(4233..4673)
                     /locus_tag="PLG42_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29664.1"
                     /translation="MKHYVTNFVRKLLIQSKNNSLITVIVLVASVIFTVNSCSDEQTI
                     MPRLTTLKANPDEITATSAILRGRITYLGNAKIIEYGIELSKSPLFSPSDTNCIKSAP
                     DTGVFAIEFKDLEPSTLYYFKAYVIINTAQYYSQNVEKFTTKSQ"
     gene            4819..5334
                     /locus_tag="PLG42_00230"
     CDS             4819..5334
                     /locus_tag="PLG42_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29665.1"
                     /translation="MWRRVKVLAERIIKNAFRESELGKILYIILPFKFYALSFWVPPL
                     IGIIVVSTKARRLSDKALTGLIAHELCHQERYRKMGVLRYYCFVLKYTFSQKARIEEE
                     HEVDRMTVRKRFAAELHELTLITHASKSHKKIISNYLTPDEIVQYAQKEGLWNSNGNE
                     TAGSKNQDSFK"
     gene            5617..>6080
                     /locus_tag="PLG42_00235"
     CDS             5617..>6080
                     /locus_tag="PLG42_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU29666.1"
                     /translation="MSNHSSLFDIIAIISLFPDLVWFGHERLMKIPLFRRVLILTGYV
                     PMRKATYRNTREMMELLKKRAESHNIAIFPEGTRTLNGKMNDFYRGFVILLRSTETDV
                     LPVTLKGFYRLKPKNRTYIDFSSTLSITIHRLIPREELIKLSDSEIILVVRSV"
     assembly_gap    6081..6090
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6175..7098
                     /locus_tag="PLG42_00240"
     CDS             6175..7098
                     /locus_tag="PLG42_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU29667.1"
                     /translation="MYKEKWVFIINPVAGKGFAASMNETIKREALKRGINPEFVFTQR
                     KGHAILLSERFVSEGYKYIIGVGGDGTFNEVATPLVGNNSVVTGLVAAGTGNDFIQIL
                     GFPDRFGDSEWDALFSAHTVAMDAGKCNNRLFFNGMGLGFDAKVASENYTPEGEVKPG
                     SKKKYIWHILKTILFYKERRMIVYNDGNMSETDCFINTVSNGRRYAGGFYLTPEAIAN
                     DGLLDICSVSKIGLYQRLRILTMVPSGRHIHDRRVHYYKTTGLLIEFSERVPYHLDGE
                     LYFDEKFEIRVIPQALNVIYNPAGRHYFNIP"
     gene            7172..7912
                     /locus_tag="PLG42_00245"
     CDS             7172..7912
                     /locus_tag="PLG42_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU29668.1"
                     /translation="MKKRFIFRTLLTLFVIFLSIPLFSQKVNTLTAKEKKEGWVLLFN
                     GKDFTGWRQYNGTGMPSNWTIEDNAMKVFTAEGKKPGQGAGGDIIYAAKKFRNFELSL
                     DWKTEKMGNSGIFYYVREVPGQAIYSAAPEVQILDNDNATDNKIASHLAGSLYDMLPA
                     DPKTCKPAGEWNNIVIRVKDGKVTHVQNGVKVVEYELWTPKWDEMVQNSKFKNFPGFL
                     EGISKEGYIGLQDHGYTIWFRNIKIREL"
     gene            8103..8915
                     /gene="zupT"
                     /locus_tag="PLG42_00250"
     CDS             8103..8915
                     /gene="zupT"
                     /locus_tag="PLG42_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOU29669.1"
                     /translation="MTENVLFALGLTLFAGLATTAGSLMAFFSRKFKPSFLCASLGFS
                     AGVMIYVAFVEIFVKANESLGNLHGEKTGYWFTVLAFFAGIALIAVVDRLVPSFENPH
                     EMKDVAGSGSIKVDNSARLHRLGLFSALAIGIHNFPEGMATFMGALHDPAIGISIAAA
                     IAIHNIPEGIAVSVPVYYSTGSRKKAFWLSFLSGFAEPVGAVAGYFILRLFFDPAGSV
                     FGLVFSAVAGIMVYISLDELLPAAEEYGRHHVAIGGVMAGMLVMALSLLLLK"
     gene            complement(9283..9353)
                     /locus_tag="PLG42_00255"
     tRNA            complement(9283..9353)
                     /locus_tag="PLG42_00255"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9319..9321),aa:Cys,seq:gca)
     gene            complement(9454..11610)
                     /locus_tag="PLG42_00260"
     CDS             complement(9454..11610)
                     /locus_tag="PLG42_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOU29670.1"
                     /translation="MKKLAIILIIFFTGAGLKALPDEGMWLLPLIEKLNIGKMTELGL
                     KLSAEDIYSVNKSSLKDAIVIFGGGCTGEIVSPNGLLLTNHHCGYGQIQAHSSLEHDY
                     LKDGFWAMTREEELPNPGLSVTFLIRIEDVTDQVLAAVKETMTENERNTAINEARMAI
                     QRKATEGTWYNATVASFYNGNQFYLFVYERFNDVRLVGAPPSAIGKFGFDTDNWEWPR
                     HTGDFSVFRVYTAPDGRPAPYSKDNIPLKPRHYLPVSIKDLNKGDFAMILGYPGRTLR
                     YYTSYEVDELLKITHPNRIKIRGIRQDILMADMQADPKVNIQYASKYSGSSNYWKYSI
                     GQKAGLEKLNVKAKKQEIEEQFNRWVNENPERKAKYGEALTLIKNAVEKRAEYAHAQQ
                     YISECFRQGCEILTMNQIATTLLSALKSGDQKRLEDLSKNIKNNITEAFYKDYNPPTD
                     KKSVKAMMKLYREDVPEKFHPDFYTNVVDKKFKGNIDRFVDDMFERSVFSEESKLLAF
                     LEKPSVKILENDPVYLTSQSIAKVSNEVNNSLSQFDNDLAKGRRLWMAALMEMYPEKT
                     FYPDANSTMRLTYGQILDYEPRDGVLYKYYTTLGGVMEKYKPGDYEFDVPQRLIDLYN
                     RKEFGRYASPKGFMPVCFTTNNDITGGNSGSPVINANGELIGLAFDGNWEAMSGDVAY
                     EPELQRTIAVDIRYVLWVMDIYAGAKHLVDEMTIVQ"
     gene            complement(11675..12274)
                     /locus_tag="PLG42_00265"
     CDS             complement(11675..12274)
                     /locus_tag="PLG42_00265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOU29671.1"
                     /translation="MLKPYLYRNLQEAGCDEAGRGCLAGPVVAAAVILPKKYRNPLIN
                     DSKKLSSKTRALLKDEITSVALAWNVAFVDNNEIDRTNIMRATIKAMHLALDGLSQKP
                     EFILVDGNVFYPYRNIKHETVVHGDSLFMSIASASILAKVFRDEYMMKIHEEFPVYGW
                     NRNKGYATSYHRDAIIKYGISPYHRKSFNLFERQLKIDF"
     gene            complement(12287..13414)
                     /locus_tag="PLG42_00270"
     CDS             complement(12287..13414)
                     /locus_tag="PLG42_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOU29672.1"
                     /translation="MKIAISARYLKEKPDGGVARFTYEVTRRIVQKNPSDEFILVFDR
                     NYSDSLLFGSNCRGVMLEPETTGPLSCYIWHEKKLPGLFETIKPDIFLSPDGVISLRC
                     RIPQVCVLHDISFYHRPQDLPLLTSLYWRYFTRKFANHAARIVTVSEFCRQDISSYLN
                     IDPGKIDIAWNGVSEYFFPAGDEEKDNYRTVLSSGIPYFLFVGNFSPRKNIPAIITAW
                     HLFKQKTRLPHRLVLAGGRLFLNRKTDRLIRSSPWRSDIILPGLIAHQDLRMLYCAAE
                     ALVFTPWFEGFGIPAAEAMRCGTPVILSPVTSLPEIGGDAALYANPADPEEICNAMIR
                     ITSEPGLRESLSVAGQTRSQKFTWDNTADCVRNAIEKVFLK"
     gene            13594..14094
                     /locus_tag="PLG42_00275"
     CDS             13594..14094
                     /locus_tag="PLG42_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU29673.1"
                     /translation="MKGKGVISAVALILFSFAVNGQQLSHQVLVPGAGLIVSGGISYS
                     QTIGETAVEIIGDFEHVLTQGFQQPRLKVTVGEAPHGNGVKVYPNPATDYVNVEFFGE
                     TSRAYSVTIININGQKIFADELDFNGSHWYVREIPLRNLARGFYFIRIVSKDELINRS
                     FKIEKM"
     gene            14106..15548
                     /locus_tag="PLG42_00280"
     CDS             14106..15548
                     /locus_tag="PLG42_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29674.1"
                     /translation="METRKFKWLFFLVILSGSVAISQEVPLGIHYQAVARDSYGKELY
                     NRDIDVKFSIYSGNPLGTLEYQELHSKVRTSRFGVFSLVIGQGINTGGRVSRLGDIEW
                     EKANHYLKVEVKFENDFMDMGTIQFLAVPYALYARKSLEPGPQGPKGDPGPQGPKGDP
                     GPPGPKGDPGDPATDDQVLSFDGTNLSISGGNTVNLSSLITPHQLTIIGDTLSISGGN
                     KVLLPNHIQDLVLNAENILKITGNVNATPINLSKYLDNTDNQKLSFNQLTGKLSITGS
                     AEEVDLSSLKNDADADPTNELQDLVLNNDELTITGKQSPNRINLARYLDNTDNQQLSY
                     NPQTNTLSLTNGGSVTLGSIVAFRARKMISTTASMATDVVFLPTVVDYNDGNAFNSLT
                     GEFIAPATGLYTFTASYYADGSGGSRKISIFLNSSLYEDLAIEIASQTQVTPRSITMK
                     LNQGDVVKLIINTGLATQTGTGTFSGFKVY"
     gene            15751..16864
                     /gene="prfB"
                     /locus_tag="PLG42_00285"
     CDS             join(15751..15813,15815..16864)
                     /gene="prfB"
                     /locus_tag="PLG42_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137305346.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOU29675.1"
                     /translation="MVTTEQIKDLNERLDALRRYLDIEGKSGEISRLETETRKPDFWN
                     DPKKAEQVLKKISALKFWVNEVNRVSSALDDLAVLYEFYREGEAPEDDVDKQYRECLS
                     LIEDLELRNMLRKEEDQLGAVLKINAGAGGTESNDWAAMLLRMYTRWAERNGYKVKQH
                     DLLPGDEAGIKSATIEIDGEKAYGFLKSENGVHRLVRISPFNAQGKRQTTFASVFVTP
                     LVDDNIEIEINPADIVWETYRSSGAGGQNVNKVETAVRLRHIPTGIVIENQESRSQLT
                     NKENALRLLKSQLYELELRKRQEEKAKLEGQKKKIEWGSQIRSYVLHPYKLVKDLRTG
                     YETSNVQAVLDGEIDGFIKAYLMEFAGKEEITPDKN"
     gene            16927..18798
                     /locus_tag="PLG42_00290"
     CDS             16927..18798
                     /locus_tag="PLG42_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOU29676.1"
                     /translation="MPVREKLFEEFPPVTSEEWMEKIVADLKGADFHRKLVWKTIEGF
                     EVMPFYRREDIQGYPHLAAGPGEFPWVRGTNTEGSGWLVRQDIEVDNFKEANRKALLL
                     LDKGVESLGFRLKDPEAVTPENVRLLLEGISPEKAEVNFICEGRAKEIVEIVSGLLRE
                     RNIPAENLRGAFEADPVGRLMVNGKLCTSVKESFDYLSELFRLATNLKAFRLIQVHAS
                     HFANAGVDTVTELAYGLAIGNEYMAALTERGISPARAASGIRFCFGLGSNYFFEIAKL
                     RAARMLWAAVVRAYVPENEDATKMRIHCVTGRWNKTIYDPYVNMLRTQTEAMAAVLGG
                     TDSLTVEPFDIIFRKPDEFSERIARNQQLLLKEEAYFSKVSDPAGGAYYIETLTHLIA
                     ENAWKLFVDTERNGGFLKSLREGIIQKRVREVAGRRLKDVAAGKEVFIGTTHYPAFDE
                     KAPDDLDIGKIYSRKSVGEGFEVEPLPTGRGTEEIEKLRLIAERASYRPVVALLPVGN
                     LAMAMARSQFSGNFFACGGYRVIDNPLFRNIDEGIKAVMKDGADIIVLCSSDEEYAEL
                     APELKEKTGDRFILVVAGNPPCMEELKQKGIKYFISIRSGLVETLSMFHRITGLIND"
     gene            18837..20954
                     /gene="scpA"
                     /locus_tag="PLG42_00295"
     CDS             18837..20954
                     /gene="scpA"
                     /locus_tag="PLG42_00295"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOU29677.1"
                     /translation="MLSDGKHFARRKDEGKSGEEWLTPEMIPIKSCYTPEDIKDFEHL
                     DYAAGIPPFLRGPYAGMYTMVPWTIRQYAGFSTAEESNAFYRRCLAAGQKGLSVAFDL
                     ATHRGYDSDHERVAGDVGKAGVAVDSVLDMKILFDQIPLREISVSMTMNGAVLPIMAF
                     YIVTALEQGAKLEELSGTIQNDILKEFMVRNTYIYPPEFSMRIIADIFRYTAEKMPRF
                     NSISVSGYHMQEAGATCDLELAYTLADGLEYLRTGINAGLDIDVFAPRISFFWAAGMN
                     HFMEIAKMRAARMLWAKIVKSFNPKNPKSMALRAHCQTSGWSLTEQDPFNNVGRTCIE
                     AMAAVFGHTQSLHTNALDEALSLPTEFSARIARNTQLFIQNETGVCRQIDPWAGSYYV
                     EYLTDSIARRAWDHIQEVESMGGMAKAIESGLPKMRIEEAAARTQARIDSGLQTIIGT
                     NKYRLEKEEPLDILEVDNSAVREAQIKRLALVRSQRDEKACQAALDALTECARTGKGN
                     LLELSIKAAACRATLGEISYALEKVAGRYKAVTRSISGIYSSEFKNDAELEEARRLVT
                     LFASKEGRQPRIMIAKMGQDGHDRGAKVVATGYADMGFDVDIGPLFQTPAETARQAVE
                     NDVHVIGVSSLAGGHKTLIPQLIAELRKLERDDILVVTGGIIPPQDYQFLYDAGVAAI
                     FGPGTSVAGAAKQIMEILLHRVE"
     gene            21029..21619
                     /locus_tag="PLG42_00300"
     CDS             21029..21619
                     /locus_tag="PLG42_00300"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU29678.1"
                     /translation="MKKLILFLLIIVLAVSGCRTGKVSFSAGPPEPVTEKKQTALPNN
                     SQQQEKIDFSKPETWILGYFSPERLNRQPHSTWFVPGYDNYQYNEEVVRKLLDLDRND
                     VSILIVMGTWCPDSRREVPRFMKILTAINFPMDRVKFLGVDNTKYTPVENYEALNIQR
                     VPTFIFYVKNIEAGRIIENPSTSLEQDMLNILTLKE"
     gene            21639..22148
                     /locus_tag="PLG42_00305"
     CDS             21639..22148
                     /locus_tag="PLG42_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29679.1"
                     /translation="MEEKRSSSGSALGTAALITGVITFVLAVIPCIGVLAIVPGIVTI
                     VLAAVAISQASRDRAPQGLIIGALVIGIVALLISTSQLFIARNLGRNADRLVPSDIRK
                     ALKEVKEDISKDVLKDLEDAKINIKIESGDEKIEINATGTAKKDKLIEQLEVLEKGEK
                     EKSDTLPGK"
     gene            22145..22585
                     /locus_tag="PLG42_00310"
     CDS             22145..22585
                     /locus_tag="PLG42_00310"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOU29680.1"
                     /translation="MTLIRKLAEAFRTKSYITINLVLAFIIAIVFLYSAVFSPQKNNY
                     PVTCIHEKITGKPCVSCGLSHSFSLIIRGKIIEAHSWNPYGFRVFLFFLAQLLMRVVF
                     SVLYLKEAEYRKQLVIYDITGTIILFLIAFYPFLAYIFSSLIQA"
     gene            complement(22588..23052)
                     /locus_tag="PLG42_00315"
     CDS             complement(22588..23052)
                     /locus_tag="PLG42_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOU29681.1"
                     /translation="MAENIKYNEALKKAMKLCAGREFCRSEIESRLEKWGIQPAEREK
                     ILKALQKDKFIDEIRFSTAYARDKFKHNRWGKVKIAVMLKSKGIDEDTIQQAVGSIDE
                     EEYINTLRKLIETHRKSVRAKNSFELRGKLVRFAISRGFEPDLIYRILEEEE"
     gene            complement(23077..23937)
                     /gene="prmC"
                     /locus_tag="PLG42_00320"
     CDS             complement(23077..23937)
                     /gene="prmC"
                     /locus_tag="PLG42_00320"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOU29682.1"
                     /translation="MNDNFRTIKDFRHYLHTRLHHLYPASEINSIADLIIMTIFNLNR
                     LQEMMKPEYEISPTRRRELLEICNNLEKGSPVQYVLGETFFYNCRIKVNPATLIPRQE
                     TEELVDLILKENPGFKGKIIDIGTGPGTIAIALAKHLPYASITAIDISDKALETAREN
                     AKLNNVKVNFFKADILKAGPEYFPEKFNILVSNPPYVPESEKKNLHINVREFEPETAL
                     FVPDDNPLIYYRAILEKKNFLLAPEGKIYFEMHEKMGKALNTLMISYGIKETRIIKDL
                     NGKERFITGK"
     gene            23964..25019
                     /gene="ribD"
                     /locus_tag="PLG42_00325"
     CDS             23964..25019
                     /gene="ribD"
                     /locus_tag="PLG42_00325"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOU29683.1"
                     /translation="MLAEADHKYMQRCIELALNAEGLTYPNPMVGAVVVHDDVILGEG
                     YHVYAGGPHAEVVAINAVKKRELLPESTLYVSLEPCCHYGKTPPCTDLIISSGIPRVV
                     VGTIDTSTKVSGRGVAILRKAGKEVEVGVMENECRWVNRRFFTYHERKRPYITLKWAE
                     SADGFIDIERPEGLSTGPYWITGMAERVLVHKWRASEEAILVGGETIRKDNPRLNVRY
                     WKGKDPVKIILSRSGNIGSYLAGKRTDGGIIVFTGNREFKPDGAEVVFLEENEPAALR
                     ICKTLYDKGIQSLFIEGGARVLQHFISEGLWDEARIFRGQKYFRNGIKAPVAGGRVIS
                     ETLFESGVLEVLSNDYL"
     gene            25100..27445
                     /locus_tag="PLG42_00330"
     CDS             25100..27445
                     /locus_tag="PLG42_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU29684.1"
                     /translation="MKGKIFYTVLVVLIATSVSFAQSGKKAYKAGNQLMKSNKYEDAI
                     VQFTNAIGAEPTNAAYYYSRATVYELTGKLNEAYADYEKSLIFKPKNVPSLVGMGRVC
                     NKLGKYDEALVYLNRAKAIEKLNREVYPEKVLALIGLEKYDQALKAADTALTIKETAY
                     NYYLRGKVYVKLNNDAMAQRELEKAIAKDRGFIEPRLELADLYLRSNKRADALNQVNE
                     LIAAAPQDARGYVVRSRIYKANLDYPNAINDISKTILLDPNNPEYYMIRGRYYQEFNQ
                     HMNAIADFSKYIAMRPDDPEAYFARAKSYQEMMNFEKAIEDYNKITTLSEFDVRARKL
                     LKEAQDMLYELNREQVPPEIEITSPVVSSGNIMEIKGNATSITVTGKIRDKSKIDEVL
                     INNQKVLTGEKKNNEAEFIATVDVRDVDRITVLARDEYNNEKVIELNLRRTEINPPKI
                     SIIAPYASEDDQIYLDTNSPRLAIEGKITDESRIKSIFIEGITAAYAVNELNPTFTAT
                     IDILNKNKITVIAEDIYGNVQEKEFRLNREGAELSASNPMGKTWVVFIENSKYTSFAP
                     LDGPVKDINMMTRALSNYQIHQIIYKKDMTKADMERFFSIELRDLIKSNQVKSLLVWY
                     AGHGKFINDVGYWIPVDARRDDEFTYFSLNTLRAAMETYINYLTHLLVVTDACESGPS
                     FYQAMRSDIKIRSCDDYTATQLKSSQVFSSAGYELAVDDSQFTRTFANTLANNPKACI
                     PIEEIVINVTNAVVNNNQQKPKFGKITGLRDEDGTFFFIAK"
     gene            27452..>29176
                     /locus_tag="PLG42_00335"
     CDS             27452..>29176
                     /locus_tag="PLG42_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU29685.1"
                     /translation="MRYLSMISAKQAIRLFLMALVFHACLLQSYAQVVYFEKYGVEQG
                     IGSSKVYTIIQDRTDFIWLGTESGVSRFDGNRFENFGSAQGLAAGGVRSIFQDTTGRI
                     WFGHLNGGISFYDGSRFRIARINSSSVSGDITGVIQTGSWLWFATSASGAYRIRFPEA
                     ADTVLHGKHYAGREGLSDQVFSIYIDRNNNLFCITDAFIKKYNAEQDTFLTYSPKGLT
                     TYFNVVAMFQDSKGNYWYGLHNGGLYRQDAATGEMKIFDILNGLSRMFISCFAEDYRG
                     NIWVGTWGGGITVFTGDKTTIYNKGNGLNALNIHSIFEDREKNILIADHYEGLSIYKG
                     DHFITYNTPDILPDNNVWAISRDETGRYWFGTNNGISVFDPIKKGKDKIQIYNEKTVM
                     IGNNIRFIIPDLGRMWIGTYGNGVYRFDLKQGRFIFDTDLNRMLPPDRIVTGMVIDKN
                     KNLWIGTNDGLARWNPSTSEGYVFTQIKGLAGNIITSLFCDSKGVIWAGSEMKTGLSK
                     LLPGADKFIVVDIGEGIIPQTIGETPDGTIWIGTVNGLIALKNEKVEKTFTENDGLLS
                     NNIKFLLSD"
BASE COUNT         8721 a         5366 c         6585 g         8494 t
ORIGIN      
        1 acgaaaagta cttttttacc ctgaaggggt gaaggtgaac cagttttctg ttgtactccg
       61 taaaattccg gtgctattaa agcagccgca gccagaacgg cacttctttt tataaaaagt
      121 cgtcttgttg tcatatgatt ttacatttca agctaagata acaaattaat aatattttat
      181 ccacattttc ttttgataga acttaaatca aaataatggg caataaatca gttaattata
      241 tttagaatca ttccaaattg aaaatttttt gcaacagcaa acacttatga acgtctttaa
      301 attgaaaaaa ttgaaaaaaa tgaaaagagt tattttatca ggatttttta tttatctgtc
      361 agttagtttt ttaatagggc aaaatgaaac agcagatatt aagaagcaat acaatgcagt
      421 ttatataaca actgctccgg taattgacgg aaagctgaac gaagatgtct ggggccaggg
      481 gaactggaat gatgatttca ttcagtttga gcctttaaac ggacgccctg ccacccagaa
      541 gacagagttc aaaatacttt tcgacgagga taatctatat atcggaataa aagcatttga
      601 caccagtcct gatagtatcg taacccgtct gacgcgaagg gatcaggctg atggagacct
      661 tgtaggtgtt attattgaca gttatcatga tcttcgcaca ggatttcttt tcggagtaag
      721 cgcgtcgggt gtaaagtttg accagatttt taccaatgat ggacagaatc aggattcatc
      781 gtgggatcca aactggtggg ttaagacatc agtaaatgat gaaggatggg tagccgaaat
      841 gaaaataccc ttcagccagt tgcgtttcga aaagaattca ggtgatgtat ggggccttca
      901 ggttgcccga atatattaca gaaagaatga aacagatttc tggcaacata ttcctagaga
      961 agcacccgga atgatacatt tattgggaga gatgaccgga cttgagaaaa tcaaaccgag
     1021 aaaaatcttt gatgtgaccc cttatgcagt tgccaaagca gagactttta aagctgaacc
     1081 tgaaaatcca tttctgaaga gcggaaaaag atttggattt aatggtggaa tagatgccaa
     1141 aataggtgtt acaaacaacc tgacaatgga tctgaccatt aatccggatt tcggacaggt
     1201 tgaagccgac ccctctgagg ttaacctgac ggcttatgaa acattcttcc gtgagaaaag
     1261 gcctttcttt atagaaggaa ataatattac aaattttgga ctgggacttg gagatggtga
     1321 tattggtaat gataatcttt tctattctag acgtatagga agaaggccac agggatttcc
     1381 atccctgaat gaagggtggc atgctgatat cccaactgct acaacaatac ttggtgctgc
     1441 aaaattaacc ggtaaaacac aaaagggtct ttctgtagga ttcattgaag cagtgacagc
     1501 tgaagaaaaa gcagaaattg acactgtggg tggaaagaaa tatgaaactg tggaaccact
     1561 tactaattat tttgttggca gagttcagaa ggattataat gaaggaaaca ctattatagg
     1621 aggtattttt acaagtacca acaggcgtct cgacgataat cttgcatctt ttatgcacag
     1681 ggaagcttat actggaggtg ttgattttac ccagtacttt aaaaagaaga gctggatgta
     1741 caatattaat actgcattca gttacgtggc aggcacaaaa gaggctattg aactcacaca
     1801 aaggtcatcc gcaagatatt atcagagacc cgacaatgat tatgtgaact ttgatccgga
     1861 ccggacctcc ctttttggct caggtgggag aatgcagatt ggtaaaatgg atggacatct
     1921 gaacctgatg ggtgctgtaa tctggaagac accaggcttt gaaactaacg acctgggtta
     1981 catccgtgaa gctgaccaga ttctctcagt gctatgggct ggatacagtc agtgggaacc
     2041 aaaaggtatt tacaggaatt acagattcgg tggtgatttt tatttcgtga acaattttgg
     2101 tggcgactgg ctgggaaaag gttttgaatg gtttgccaat atgaacttca agaattactg
     2161 gtttgcctgg acaggaggca atataaacac tacccagctt tcaacaggta tgcttcgcgg
     2221 aggtccaatg ataaaactac ctggcaacat taatatgaga cttggctttt caactgacaa
     2281 caggaagaaa cttgttctgc aggtatctgc caacaattca acagggctgg aagggaattc
     2341 atcaagctgg tataccatgg ctgatatatc gtttaaacct accaattacc tgagagtctc
     2401 attcaacccc tcatataacc cttcatttac tgaattacaa tacgttacaa aaaaatcttt
     2461 cggaaatgac acaagatatc tttttgcaag gattgacagg aaaacactca gcgcatcgtt
     2521 aagagtggat tttaacctca cacctgaact tacgcttcag tactggggtc agccatttat
     2581 tgccacagga aaatactccg aattcaaata cattaccaat ccaatggctg atgaatataa
     2641 agaccgtttc catgtctata caccagctca aataagtttg aattcaggca gttacgatat
     2701 tgatgaaaac tccgacggaa caactgatta taatttcgga aaacccgatt ttaatgttca
     2761 ggagttcctt tcgaaccttg ttattagatg ggaatataat ccgggttcga caatttatct
     2821 ggtgtggagc cagactcgca gttgtttcaa tgattcaggc cagatggatg tttttaacga
     2881 cctgggcgac cttttcaatt gcaaagacaa taaacctcat aatgtctttc ttgttaagtt
     2941 cagttacaga tttggtttaa ggtaaaaaga attatgccca ggaaggcata attggctgaa
     3001 aaacagttat cttattgtga agcaaaccag gtaatttggc gaacaatcag tgaaggatct
     3061 gaacctttag gggcaggtcc ttctgatttt atttatttaa aagtcgagac atctcgttaa
     3121 acatatccct gaaacagttt gctgcagtgt tttcagaagc ttcagtagat ttaccgtaat
     3181 agctgtcgag aaactgtttc actcccgacc ataccgtctg gaccacacct ttaaaatgtt
     3241 ctttcatttc aggagttgat gaatttctga aaagaatgat attgtttatc tgggcaaccc
     3301 ctacttctgg ttttctccac ggacatgaaa ccacatcgaa acctttcatg gcaaaatata
     3361 ctgctgtggg taaggggctt tcataatgcc agtcgcatat tacaacatct ttcggaatca
     3421 tgtctattgc tctgtgggta ttgttaaagc ttgcttccca ttcgcctatt cctgttgttt
     3481 tgccgtcaat caatctgtcg ccccatatcc agagccttct ccctttctgt gccaggtgat
     3541 ttcgtatcag agtcacttcc cctgcaaata attcagcttt atctcttcct ccgcagcggg
     3601 gacactggtc ttcccctatg taaaaaactt catccatacc tgcatgaaat gctccagctt
     3661 caaaaacatc acagatttcg tcaacaagtt cgaatactat cttatgtact tcaggatgaa
     3721 ggggacaata gcttttacag taaagtctgt cggggtttgg ccatgaatat ttttcgggca
     3781 tcttcaccca tggggtttca tcaaactgtg ggtagaccct taaaaggttc cctgtgcggt
     3841 ttgcccacga ctggtggccc agaaggttga tctgtggaat aattgttatc ttattgtctc
     3901 tgcaaacctc aaccagtttt ttgacatctc tttttgagag agctattgaa tctctgagtt
     3961 cgggatggct tttatattca taattgtaat ccactctcag aatcagggta ttgacttttc
     4021 tgggaacaag ttcttcttta ataaaagtta taaaatcatc cagttctgct tttctcggtg
     4081 ctgctatgca aaagcctctt acagggacca gagtgtcaag tgcttcctgt gaatatgaat
     4141 agattgctgg tatgcagaat gtaaggagaa ttgtaaagac ttgcctcatc gaaaggaagt
     4201 tttgacaacg atgccaggtc atagtagagg ttttattgtg attttgttgt gaatttttca
     4261 acgttctggg agtaatactg tgcagtattt atgattacgt aggctttgaa ataataaagc
     4321 gttgaaggtt ccagatcttt gaattcaatt gcgaatacac ctgtatcagg tgcacttttt
     4381 atgcagttcg tgtcggatgg agaaaagagc ggactctttg acagctctat gccgtattct
     4441 atgattttag cattaccaag ataggtaatc ctccctctaa gaatagctga ggttgccgta
     4501 atctcatcag gattagcctt aagagttgta agccttggca taattgtttg ttcatcggag
     4561 cacgaattca ctgtgaatat aacagatgca accagaacaa ttacagttat taaactgtta
     4621 tttttagatt gtatcagaag tttcctgacg aaattagtaa cataatgttt catagcaatt
     4681 tttttggctg attacaaata tacattttca gttttttatg tacaaaatct tcaaatgaaa
     4741 ggtgctacat cagttaatca attaatggtt ataacccttt attattatat ttgagtcgtt
     4801 tgaattttct ttatggttat gtggagaaga gtaaaagtgc tggcagagag aataataaaa
     4861 aatgccttca gggaatctga attgggtaaa atattataca taatccttcc cttcaaattt
     4921 tatgcgcttt cattctgggt tcctcccctg ataggtataa ttgtagtaag cactaaagca
     4981 agaagattaa gtgataaggc acttaccggc ctgattgcac atgaattgtg ccaccaggaa
     5041 agataccgga aaatgggtgt tttaagatat tattgttttg tgctgaaata tacattttct
     5101 cagaaggcaa ggattgaaga agaacatgag gttgacagaa tgactgttag aaagagattt
     5161 gctgcagagt tgcatgagct tactcttata acacatgcca gcaaaagcca taaaaagata
     5221 atcagtaatt atcttactcc tgatgaaatc gtccaatatg cacagaaaga gggattatgg
     5281 aacagtaacg gtaatgaaac agcaggaagc aaaaaccagg atagttttaa ataaaaataa
     5341 tttttatata cttttacttt tcatgtatta ttaagggttt aacatttaat gtccaaaaaa
     5401 tattttttca gtgctctttc tgtcccggtt tatggactct tgtatctttt cacaattctg
     5461 atgatattgc ttggtcttcc ctttatgttt tccaggaagg agctcattgt aagggctatg
     5521 atgaggttct gggccaaagg cattttcata ttgatgggta gaaaaataag tgttatccgg
     5581 taagaaaatc aggagtatag gcggcattat attcttgtgt ccaaccattc gagtcttttt
     5641 gatattatag ccataatatc gttgtttccg gatctggtat ggtttggtca tgaaaggctg
     5701 atgaaaatac ctctattcag aagggtgttg atacttaccg gttatgttcc gatgaggaaa
     5761 gccacatacc ggaataccag ggaaatgatg gaacttttga agaagagagc agaaagtcac
     5821 aacattgcta tttttcctga aggcacaagg acccttaatg gtaagatgaa tgatttttac
     5881 aggggctttg tcattcttct ccgttccact gaaacggatg tccttcccgt tactctaaaa
     5941 ggattttaca ggctgaagcc caaaaacaga acttatatag atttcagctc aactttgagt
     6001 ataaccatac acagacttat accacgggaa gaacttataa aactgagcga cagtgaaata
     6061 atactggttg tcaggtcagt nnnnnnnnnn cagtaattga aaaatctttg accgaagaca
     6121 acggggttta taatggaagc agggtattta taaaaaataa atcagcagtt tataatgtat
     6181 aaagaaaagt gggtattcat aattaatcct gtggcgggca aaggttttgc tgccagtatg
     6241 aatgagacaa ttaaaagaga ggctttgaag agaggtataa atccggaatt tgtatttact
     6301 cagagaaagg ggcatgccat acttctttcg gaaagattcg tttcggaagg ttataaatat
     6361 ataataggtg ttggaggtga cggaacattc aatgaagtgg caacgcctct ggttggcaat
     6421 aatagcgtag taacaggtct ggttgctgca ggaaccggaa atgatttcat tcagatatta
     6481 ggtttccccg acaggtttgg tgacagcgag tgggatgccc ttttcagtgc acatactgtg
     6541 gcgatggacg ccgggaaatg taataaccga ctctttttta acggaatggg gctcgggttt
     6601 gatgcaaagg tggcttcgga aaactataca ccggaaggag aagttaagcc aggcagtaaa
     6661 aagaaatata tctggcatat cttgaagacg atattgtttt ataaagagcg caggatgata
     6721 gtttataatg atggaaacat gtctgaaaca gattgtttca tcaatacggt ttcaaatgga
     6781 cgaaggtatg caggtggatt ttatcttact cctgaagcta tagcaaatga cggtttgctg
     6841 gatatctgca gtgtcagtaa aatcggactt tatcagcgtc tgagaattct tacaatggtt
     6901 ccctcaggca ggcacattca tgacagaagg gttcattact acaaaactac aggtcttttg
     6961 attgagttca gcgaaagggt gccttaccac ttagacgggg aactctattt tgatgaaaaa
     7021 ttcgagatta gagtaattcc tcaggcactc aatgtaattt ataaccctgc cgggagacat
     7081 tattttaata taccctgaaa ttgtacagtt caatttatat aatgattatt taaaagctta
     7141 attttgtgaa tacgaactaa aacttagaac tatgaaaaaa agatttattt tcaggacttt
     7201 gttaacatta tttgtaatat ttttatcaat tcctcttttt tcacagaagg taaatacgct
     7261 tactgcaaag gaaaagaagg aaggatgggt cctgcttttc aacggtaagg attttacagg
     7321 ctggaggcag tataatggca ccggcatgcc gtcaaactgg accatcgaag acaatgctat
     7381 gaaggttttt actgctgaag gaaagaaacc tgggcagggt gccggaggtg atattattta
     7441 tgcagctaag aaattcagga attttgaatt gtcactcgac tggaaaactg agaaaatggg
     7501 taattcgggt attttttatt atgtgaggga agtgccagga caggcaatat attcggctgc
     7561 tccagaggta cagatcctgg ataatgacaa tgcaactgac aacaaaattg caagccatct
     7621 ggcaggttca ctgtacgaca tgctacctgc cgatccaaag acatgcaaac cggccggaga
     7681 atggaataat attgttatca gggttaaaga cggtaaggta acgcatgtcc agaacggtgt
     7741 caaggttgtt gagtatgaac tgtggactcc caaatgggat gaaatggtac agaacagcaa
     7801 gttcaaaaat ttcccggggt ttctcgaagg catttcaaaa gaagggtata tcggccttca
     7861 ggatcatgga tatacaatct ggttcaggaa tattaagatt agagaacttt gagattgtaa
     7921 gaattaggaa tagttagtta tttaattatc aatccccgga taagtcccgg ggatttttta
     7981 tacatattat ttcacggcaa tatattgtca gataaaatca tttttaatta tctcgattac
     8041 ggaacatcgc cggtaaagat ctgaagcaag tctgaaaatt atttacattt gctggtgaaa
     8101 tgattactga aaacgtatta tttgcgcttg gtttaactct ttttgcagga cttgcaacaa
     8161 cagcaggcag tctgatggca ttcttttcaa ggaagttcaa gccttcattt ttatgtgcat
     8221 cactgggttt ttctgccggg gtaatgatat acgtagcttt tgtggaaatt tttgtgaaag
     8281 cgaatgagtc acttggtaat ctacacggtg aaaagacagg ctattggttt actgttcttg
     8341 ctttttttgc aggcattgca ttgatagctg ttgttgaccg actcgttccg tcatttgaga
     8401 atcctcatga gatgaaagat gtagctggaa gtggttctat aaaagtggac aactcagccc
     8461 ggcttcaccg gcttggactc ttttccgctc ttgccatagg aattcacaac ttccctgaag
     8521 gtatggctac gtttatggga gcacttcacg accctgcaat agggattagt attgcagctg
     8581 cgattgcaat tcataatatt ccagagggga tagctgtttc tgtgcccgta tactattcga
     8641 ccggaagcag gaagaaagct ttctggctct cttttctttc aggttttgcc gaaccggtag
     8701 gggctgttgc cggatacttt atacttagac tattctttga tccggcaggc tcagtcttcg
     8761 gacttgtctt ttcagctgtt gcaggtataa tggtttatat atctctcgat gaattgcttc
     8821 ctgcagcgga agagtacggg cggcatcatg tagcaatagg aggagtaatg gctggaatgc
     8881 tggtaatggc tttgagcctt ttacttctta aatagcgatt aatatttttg atgattttgc
     8941 tgaggtcggt ggcggattac ctgagggtga tccggtttgg ggtgactaat cagcaaaatc
     9001 ccatgccgct ttcagcggtc aggtcttttt tccttacact cctccgcttc gcaagcgagc
     9061 ttcgtcctgt aagaaaaaaa tccctggcac ttcgtgccat gggattttgc tgaggtcggt
     9121 ggcggattac ctgagggtga tccggtttgg ggtgactaat cagcaaaatc ccatgccgct
     9181 ttcagcggtc aggtcttttt tccttacact cctccgcttc gcaagcgagc ttcgtcctgt
     9241 aagaaaaaaa tccctggcac ttcgtgccat gggattttgc tgaggtcggt ggcggattcg
     9301 aaccgccgta aacggttttg cagaccgacg cctagccact cggccaaccg accgttttag
     9361 actgcaaatt taataaaata ttcgattaac cttaaaaata aaggggatta attacttaat
     9421 cccctatgca acagttaatt atattaaagt atcttattgt actatagtca tttcatccac
     9481 caggtgtttt gcacctgcat atatatccat tacccacaaa acatatctta tatcaacggc
     9541 aatggttctc tgaagctccg gctcatatgc cacatcgccg ctcattgctt cccagtttcc
     9601 gtcaaatgca agccctataa gttcaccatt ggcattgata accggactgc cgctattgcc
     9661 tcctgtgata tcgttgttgg ttgtaaagca aaccggcata aatcctttag gcgaagcata
     9721 tctgccaaat tccttacggt tgtaaaggtc tatcagccgc tggggaacat caaactcgta
     9781 atcgcccggt ttgtatttct ccatcactcc tccaagggtt gtataatact tgtataggac
     9841 cccgtcgcgt ggttcataat ccagtatctg gccataggta agacgcatgg tggaatttgc
     9901 atcaggataa aaagtctttt caggatacat ctccatcagg gcagccatcc ataacctgcg
     9961 tccttttgcc agatcgttat caaattgact gagactgttg ttgacttcat tgctaacctt
    10021 tgcaatggat tgggatgtaa gataaacagg gtcgttttca agtatcttaa ctgatggctt
    10081 ttcgagaaat gcaagaagtt ttgattcttc cgaaaaaact gacctttcaa acatatcatc
    10141 tacaaacctg tcgatattcc ctttgaattt tttatcaaca acgtttgtat aaaaatcggg
    10201 atgaaatttt tcaggtacat cctcacggta aagtttcatc attgctttaa ccgacttttt
    10261 atcggtagga ggattgtaat ccttatagaa agcttctgta atgttgtttt taatattttt
    10321 tgagaggtct tcaagacgtt tctggtcgcc tgattttagg gctgacagca gtgttgtagc
    10381 tatctggttc attgtcagta tttcacagcc ctgacgaaaa cattcgctta tgtactgctg
    10441 tgcgtgagcg tattctgccc ttttttcaac agcgtttttt atcagggtta gagcttctcc
    10501 gtattttgct tttctttcgg gattttcatt tacccatctg ttgaactgtt cttcaatctc
    10561 ctgttttttt gcctttacat tgagtttttc cagcccggct ttctgcccta tggaatattt
    10621 ccagtaattt gacgaccctg aatatttgga tgcatactga atgtttactt tcgggtcagc
    10681 ctgcatgtcg gccataagta tatcctgtcg gatgcccctt atttttattc tgttcggatg
    10741 cgttattttc aggagctcat ctacttcgta agatgtataa tatctgagag ttcttcccgg
    10801 gtatccaagt atcatggcaa agtcgccctt attaaggtct tttattgata caggcaggta
    10861 atgtcttggt ttaagaggga tgttatcttt tgaataagga gcagggcggc catcgggagc
    10921 agtatatacc ctgaaaacac tgaagtcgcc ggtatgccgt ggccattccc agttgtcggt
    10981 gtcaaatccg aatttaccta ttgccgaggg tggtgcgcca accaaacgta cgtcattgaa
    11041 tctttcataa acaaacaggt agaactgatt gccgttgtaa aacgaagcaa ctgttgcatt
    11101 gtaccatgtt ccctcggttg ctttcctctg tatagccatt cgtgcctcgt ttattgccgt
    11161 gtttctctca ttctcggtca tagtctcttt aacagcagca agtacctggt cggtaacatc
    11221 ttctattctt ataaggaaag tgactgaaag tccggggttg ggaagttctt cttcgcgggt
    11281 catagcccag aaaccatcct tgaggtaatc atgttcgagt gagctgtgtg cctgtatctg
    11341 cccgtagcca cagtggtggt ttgtaagcaa tagaccattg ggagaaacga tttctccggt
    11401 acagccacca ccaaatatta caatggcatc tttcagactt gatttgttta cactgtagat
    11461 atcttctgca ctgagtttta agcccagttc agtcatttta cctatgttaa gcttttcaat
    11521 cagcggcaga agccacattc cttcatcagg tagagctttt aaccctgctc ccgtgaaaaa
    11581 gataataagg atgattgcta attttttcat gattttaaat gatttgtttc agaataggga
    11641 gtaaaaataa atctttaagt tcataaaaac catctcaaaa gtcaattttt aattgtcttt
    11701 caaacaaatt gaatgatttt ctgtgatatg gggatatacc atattttata atagcatcgc
    11761 ggtgataaga agttgcatag cctttgttac ggttccaacc gtaaaccgga aattcttcat
    11821 gtattttcat catgtactca tcacggaaca cctttgcgag tattgaggct gaggctattg
    11881 acataaaaag agagtcgcca tgcaccacag tttcatgttt aatattcctg taaggataaa
    11941 aaacgttgcc atcgacaagg ataaactcag gtttctgact gagtccatca agtgccagat
    12001 gcatagcttt tattgtagct cgcatgatat ttgtccggtc aatttcgttg ttatctacaa
    12061 aagcaacatt ccaggcaagt gcaacggagg ttatctcatc cttcagaaga gccctggttt
    12121 ttgaagagag tttttttgag tcgttgataa gagggtttct gtatttttta ggtaggataa
    12181 ccgctgctgc cacaacagga cccgccaggc aaccccttcc ggcttcatca catcctgcct
    12241 cctgaaggtt acggtagaga taaggtttca gcattgctga taatatttat tttaaaaata
    12301 ctttttcgat tgcatttctg acgcaatctg cagtattgtc ccaggtgaat ttttgtgacc
    12361 ttgtctgacc ggcaactgat aatgattctc tgagccctgg ttctgaggtt attctgatca
    12421 ttgcattgca tatttcttca gggtcagcag ggtttgcata taacgctgca tctccgccta
    12481 tttcagggag ggatgtgaca ggtgagagta taacgggagt tccgcatcgc attgcctcgg
    12541 cggctggtat tccgaaccct tcgaaccatg gtgtgaaaac cagtgcttca gcagcgcaat
    12601 acagcattct aagatcctga tgtgctatca aacctggaag gataatgtca ctacgccatg
    12661 gagaagaacg gatcaacctg tcggtcttcc gatttaaaaa gagacggcca cctgccagaa
    12721 caagtctgtg agggaggcgg gtcttctgtt tgaaaagatg ccaggcagtt attattgcag
    12781 gaatattttt ccgcggggaa aaatttccca cgaaaagaaa atatggaatt ccactgctaa
    12841 gaactgttct gtaattatct ttttcttcat cacctgccgg aaagaaatat tccgatacgc
    12901 cattccaggc aatgtctatt ttaccgggat ctatattcag atatgaagat atatcctgcc
    12961 ggcaaaattc tgacacagta acaatacggg ctgcatgatt ggcaaacttt ctggtgaaat
    13021 atctccagta aaggcttgta agtaagggta aatcctgtgg tctgtggtaa aaactgatat
    13081 cgtgtaaaac gcacacctgg gggatacgac acctcagtga aataactcca tcgggcgaaa
    13141 gaaagatatc tggcttaatt gtctcaaaaa gtccgggtaa ttttttttca tgccatatat
    13201 aacatgataa tggacctgtt gtttccggtt caagcataac gcccctgcag tttgagccaa
    13261 atagcaggga atcgctgtag tttctgtcga aaaccagtat aaactcatcc gatggatttt
    13321 tttgtacaat tcgcctggta acttcataag taaatcgtgc aacaccaccg tcgggttttt
    13381 ccttaagata ccgtgcactt atagcaatct tcatttttat ccagagataa taggaagagg
    13441 ctttaaagaa aataaaaaat accctaccag atgcattttg cttgaaagtt aacctgaata
    13501 tgggcattat tatgtgtttt taacaaaaat tatcaatatt gttgataatt tgttaaaaaa
    13561 tattttattt tggggaaatt ctggatttta ataatgaagg gaaagggggt tatttcagca
    13621 gttgcattga tactattttc ttttgctgta aacggacagc agctatctca tcaggtgctg
    13681 gttccggggg caggattaat cgtgtcagga ggaataagtt attcccagac tattggtgaa
    13741 acggccgttg aaataatagg agattttgaa catgttctta ctcagggatt tcaacaacca
    13801 cggttaaagg taacagtggg tgaagctcct catggaaatg gtgtaaaagt ctatcctaat
    13861 cctgcaactg attatgtgaa tgtcgaattc tttggtgaaa cttcacgtgc ctacagtgtt
    13921 acaataatca atataaacgg acagaagatt tttgccgacg aacttgattt caatggctct
    13981 cactggtatg tgagagaaat acccctgagg aatttggcaa ggggttttta ttttataaga
    14041 atagtaagca aggatgaatt aattaacagg tcgttcaaaa ttgaaaaaat gtaatctcag
    14101 aatatatgga aaccagaaaa tttaaatggt tgttttttct tgtaattctg tcgggttctg
    14161 ttgcaatatc ccaggaagtt ccccttggta ttcattatca ggcagttgca cgcgacagtt
    14221 atggaaagga attgtataac agggatatag atgtaaagtt ttccatatat tcaggtaatc
    14281 ctcttggtac gcttgagtac caggagcttc attcgaaagt gaggacatca aggtttggag
    14341 tcttttctct ggtgataggt caggggatta acaccggagg aagggtttcg cgtcttggtg
    14401 atattgagtg ggagaaagca aatcattatt taaaggtcga ggttaagttc gagaatgatt
    14461 ttatggatat gggcaccatt cagtttcttg ccgtgccata tgctctttat gccaggaagt
    14521 cgcttgagcc cggcccgcag ggaccaaagg gtgacccggg cccgcagggg ccaaaaggtg
    14581 atcccggccc gccaggacca aaaggcgacc ccggcgaccc tgcaactgac gaccaggtac
    14641 tctcatttga cggtacaaat ctctcaataa gcggaggcaa tacggtaaat ctttcatctc
    14701 taattactcc gcatcaactc acaataatag gagatacatt gtcaatttca ggagggaata
    14761 aagtactgct gcccaatcat atacaggatc ttgtactcaa tgcagaaaat attcttaaga
    14821 taacaggaaa tgtgaatgcc acaccgataa acctttcaaa atatcttgac aataccgata
    14881 accagaaact gagttttaat caactaacag gtaaactatc cataacaggt tctgcagaag
    14941 aggtcgacct ttcatcactg aaaaacgatg ctgatgccga tcccacaaat gaattgcagg
    15001 accttgtgct gaacaatgat gaacttacaa ttactggaaa acaatcgccc aaccggatca
    15061 atcttgccag gtacctcgat aacaccgata accagcaact cagttataac cctcaaacca
    15121 acaccctctc acttaccaac ggtggttcag taacactggg aagcattgtg gcattcaggg
    15181 caagaaaaat gatttcaaca acagcctcga tggcaactga tgttgtattt ttaccgacag
    15241 tagttgatta taacgacgga aatgcattta acagtttgac aggtgaattc atagcaccag
    15301 ctacagggct atacactttc actgcttcat attacgcaga tggttctggc gggtcaagga
    15361 aaatatccat atttcttaat tcaagtttat atgaagatct tgcaatagaa atagcttctc
    15421 agacgcaggt cacaccccga tcgattacca tgaagttgaa tcagggtgat gttgttaagc
    15481 tgataataaa taccggattg gcaactcaaa ccggtaccgg caccttctca ggtttcaagg
    15541 tttattgaat ttgaatagaa ttatacccca gcccagatag tttattccat tccatggagc
    15601 tatcttaaaa gtttttcttt ttgtttttca taaattcttc cagcatgtgg ggaacaggta
    15661 aatggataaa ttcccttcca gaccaatctg ccagcaggaa ataaaaacat ggaaaattat
    15721 taactttgca cctcaattat ttatgtgaga atggtaacaa cagaacagat aaaggatctg
    15781 aacgaacgcc tggatgcgtt gaggaggtat ctttgacatt gaaggcaagt cgggcgaaat
    15841 aagcaggctc gagactgaaa cgagaaagcc agatttctgg aatgatccca aaaaagccga
    15901 gcaggttctc aagaaaatat cagcattgaa attctgggtt aatgaagtaa acagggtctc
    15961 atctgctctg gatgaccttg ctgtgctgta tgagttttac agggaaggag aggctccgga
    16021 ggatgatgtg gataaacagt acagggaatg tttatcgttg attgaggatc ttgaactgag
    16081 gaacatgtta aggaaggaag aagatcagct gggtgcagtg ctgaagatta atgcaggtgc
    16141 aggtggcact gaaagcaacg actgggcagc aatgcttctc aggatgtata cacgctgggc
    16201 agaacgtaat ggctacaaag tcaaacagca cgatctcctt ccgggagatg aagctggaat
    16261 taaatctgcc accatagaga ttgacggaga aaaggcttac ggattcctga aaagcgaaaa
    16321 cggggtgcac cgtcttgtaa ggatttcacc attcaatgca cagggaaaac gccagacgac
    16381 atttgcttca gtgtttgtta caccacttgt tgacgacaat attgaaattg agatcaaccc
    16441 tgctgatatc gtatgggaga cctacagatc gagcggagcg ggagggcaga atgtaaacaa
    16501 agttgaaacc gctgtgcggc tcaggcacat tccgaccggt atagtaattg aaaaccagga
    16561 gtcgagatct cagctcacca acaaggaaaa cgccctgcgg ttgctgaaat cgcaactgta
    16621 tgagcttgag cttagaaaga ggcaggagga aaaagcaaaa cttgaaggtc agaagaaaaa
    16681 gatagaatgg gggtcgcaga ttcgctcata tgtgttacat ccttataaac tggtaaaaga
    16741 cctgcgtacc ggatatgaaa cctccaatgt tcaggcagtt cttgatggtg aaattgacgg
    16801 attcataaaa gcatacctta tggaatttgc cggcaaggaa gagataaccc cggataaaaa
    16861 ctgatataat tcataaagtt gttgagttat aattgcttag tttgttgaat cattattata
    16921 ccttttatgc ctgttcgtga aaaactgttt gaagaatttc ctcccgtgac ttcagaggaa
    16981 tggatggaaa aaatagttgc cgacctgaaa ggtgcagatt tccacagaaa gcttgtctgg
    17041 aagaccattg aaggttttga agtgatgccg ttttaccgca gggaggatat tcagggttat
    17101 ccgcatcttg cagctggtcc tggtgaattt ccatgggtta ggggaacaaa tactgaagga
    17161 agcggttggc tggtcaggca ggatattgag gttgacaatt ttaaggaagc aaacagaaaa
    17221 gcccttctac ttctggataa aggtgtcgaa tccctggggt tcagactcaa ggaccctgaa
    17281 gctgtaacac cggaaaacgt ccgattgctt ttagaaggaa tctctccgga aaaggctgag
    17341 gttaatttca tttgtgaggg aagagcgaag gaaattgtcg aaattgtttc cggtttgctg
    17401 agggaaagga acataccggc agagaatctc agaggtgcgt ttgaggcaga tccggttggt
    17461 cggcttatgg ttaacggaaa actttgtaca tcagttaaag aaagctttga ttacctgtca
    17521 gaactattca ggctggcaac caatttaaag gcattcagat taattcaggt gcatgcttcg
    17581 cattttgcaa atgcaggtgt agatacagta actgaacttg catatggtct tgccattggg
    17641 aatgagtata tggcagccct gactgaaaga gggatatcgc ctgcacgggc agcttcaggc
    17701 ataaggtttt gctttggatt gggttcaaat tatttctttg aaatagcaaa actccgtgcg
    17761 gcacgtatgc tgtgggctgc ggtagtaagg gcttatgtgc ctgaaaatga ggatgctaca
    17821 aagatgagaa tacattgtgt tacaggcaga tggaataaga ccatatacga tccgtatgtt
    17881 aacatgcttc gcacgcaaac tgaagctatg gccgcagtgc tcggcggaac agattcacta
    17941 acagttgaac cttttgatat tattttcaga aagcctgatg agttctcgga gagaattgcc
    18001 cgcaaccagc agcttcttct gaaggaagag gcgtatttca gtaaagtatc agaccctgca
    18061 ggaggggcat actatatcga aactcttacg caccttattg ccgaaaatgc ctggaaactc
    18121 tttgttgata ccgaaagaaa tggaggattc ctgaaatcac tcagggaagg cattatccag
    18181 aaaagagtca gggaggttgc aggcaggaga ttgaaagatg tggctgcagg taaagaggta
    18241 tttattggta caacacatta tcctgctttt gacgaaaaag cacctgatga tctcgatatc
    18301 ggaaagattt atagcagaaa gagtgttgga gaaggttttg aagttgaacc tttgccgaca
    18361 ggcaggggaa ctgaagagat tgaaaaactg agacttattg ccgaaagggc atcgtacaga
    18421 cccgttgttg ctcttcttcc ggtcggtaat cttgccatgg caatggcacg ttcccagttc
    18481 tcaggaaatt tctttgcatg cggaggttac agggtaattg ataatcccct tttcagaaac
    18541 attgatgagg gtattaaagc cgtaatgaaa gacggcgcag atataattgt tctgtgcagt
    18601 tctgatgaag aatatgccga acttgctcct gaactgaagg aaaagacagg tgacaggttt
    18661 attcttgtag tagcaggaaa ccctccatgc atggaagaat taaagcagaa aggaattaaa
    18721 tattttataa gtatacgttc gggacttgtt gaaacacttt cgatgttcca tcgtatcacc
    18781 ggacttatta atgattaaag atgaggcctg aattcagata tgatgattta gacaagattt
    18841 tatctgatgg caaacatttt gcccgcagga aagatgaagg taaatcaggt gaggaatggc
    18901 ttacgcccga aatgattccg ataaagagct gttatacacc tgaggatatc aaagactttg
    18961 agcatcttga ttatgcagct gggatacctc cttttctcag gggaccttat gccggaatgt
    19021 atacgatggt accctggact atcaggcagt atgcaggttt ttccactgcc gaggaatcta
    19081 acgctttcta caggcgttgt cttgcagcag gccagaaagg gctttctgta gcatttgacc
    19141 ttgccactca ccgcggatat gattcggatc atgaaagggt tgcaggagat gtaggtaagg
    19201 caggtgtagc ggtagattcg gtgctcgata tgaagatact ctttgatcag atacctctca
    19261 gggagatttc ggtatctatg actatgaacg gggccgtact gccgattatg gcattttata
    19321 tcgttacggc acttgagcag ggtgcaaaac tcgaagaact ttcgggtacc atccagaacg
    19381 atatattaaa ggagttcatg gtcagaaata catatatcta tcctcctgag ttttcgatga
    19441 ggataatagc tgacatcttc aggtatacag ctgaaaagat gccgaggttc aattcaataa
    19501 gcgtatcggg ttatcacatg caggaagctg gggccacctg tgatcttgag ctggcatata
    19561 cccttgccga tgggcttgaa tatctgagga caggcataaa cgctggcctc gatattgacg
    19621 tttttgcacc aaggatttcg tttttctggg ccgcagggat gaatcatttt atggaaattg
    19681 cgaagatgag ggcagcaagg atgctctggg ccaagatcgt aaagagcttt aatccgaaaa
    19741 atccgaagtc gatggctttg agggcacact gtcagacgtc gggctggagt cttactgaac
    19801 aggatccttt caacaatgtt ggccggacat gtattgaagc gatggcggcc gtttttggac
    19861 atacgcagag cctgcatacc aatgctctcg atgaggctct gtcactgccc acggagtttt
    19921 ctgcaagaat tgcccgcaat actcagttgt ttatacagaa tgaaacaggc gtttgcagac
    19981 agattgatcc atgggctgga tcgtattatg ttgaatattt gactgattcg atagcccgca
    20041 gggcctggga tcacatacag gaggttgaaa gtatgggggg aatggcaaaa gcaattgaat
    20101 cgggtctgcc aaagatgaga attgaggaag ctgcagcaag aacacaggca aggattgact
    20161 caggtttgca gacgattata ggtacaaaca agtacaggct ggaaaaggaa gaacctcttg
    20221 atattctgga ggttgacaac agtgctgttc gcgaagccca gataaaaaga cttgctctgg
    20281 tgcggtcgca gagagatgaa aaagcatgtc aggcagcact tgatgccctt acggaatgtg
    20341 ccaggacagg taagggtaat cttcttgaac tttcgataaa agctgcagcc tgtcgtgcaa
    20401 cacttggaga aatttcatat gcactcgaaa aagtagcagg gagatataaa gccgtgacac
    20461 gttcaatttc aggaatttac tcatcagagt ttaaaaatga tgcggagctg gaggaagccc
    20521 gcaggctggt gactctcttt gcctcaaaag aaggcaggca gccaagaata atgattgcta
    20581 aaatgggaca ggatggccac gaccgtggtg caaaggtggt agctacagga tatgccgaca
    20641 tgggttttga tgtggatata gggccattgt ttcagacccc tgcagaaaca gcacgccagg
    20701 cagtggaaaa tgatgtccac gtaatcggtg tgtcgagcct tgccggaggc cataaaactc
    20761 ttataccaca actaattgca gaactcagaa aactggaacg ggatgatatc cttgtagtta
    20821 caggagggat tattccacct caggattatc agtttctcta cgatgcaggt gttgctgcta
    20881 tcttcggccc cggaacctcg gtggcaggag cagcaaaaca gattatggaa atattgcttc
    20941 acagggtaga ataaaattac ccctgagaat caaaaaactt gtgtttattt cataattaac
    21001 ataacttagg cttgttttat aagtgcatat gaaaaaactt attctctttc tattgataat
    21061 agttttagca gtttctggat gcagaaccgg caaagtttca ttctctgctg gacctccaga
    21121 accggttaca gagaaaaaac agacagcttt acctaataat tcgcaacagc aggaaaagat
    21181 tgatttttca aagcccgaga catggattct tggctatttt tcacctgaga ggttaaatcg
    21241 ccagccacac tcaacatggt ttgtaccggg atatgacaat tatcaataca atgaagaagt
    21301 tgtcagaaaa cttctggatc ttgacaggaa tgatgtttca atacttattg tgatgggaac
    21361 atggtgccct gacagccgca gggaagtgcc tcgttttatg aaaattctga cggcaattaa
    21421 ctttcccatg gacagagtga agtttttagg tgtggataat acaaagtata ctccggttga
    21481 aaattatgag gctcttaata ttcagcgagt gccaacattt attttttatg taaaaaatat
    21541 tgaggcagga cgcatcattg agaatccttc gacgtcttta gaacaggata tgctgaatat
    21601 tctgacatta aaagagtaaa atttaaaaaa taagtaagat ggaagaaaaa agaagcagtt
    21661 cgggatcggc acttggtaca gctgcattaa ttacaggtgt tatcacattt gtacttgcag
    21721 ttataccatg tataggggta cttgctattg ttccgggcat cgtaacaata gtgcttgccg
    21781 cagtggctat ttcgcaagct tcaagagaca gggcaccaca gggtttaatt attggtgcac
    21841 tggttatcgg tatagtggca cttcttattt caacctcaca gctgtttatt gccagaaacc
    21901 tgggacgtaa tgctgacagg ctggttcctt cagacatcag gaaggcgctg aaggaggtga
    21961 aagaagatat ttcgaaggat gtattgaaag acctggagga tgccaaaata aatattaaaa
    22021 ttgaaagtgg cgatgaaaaa atagagatta atgccaccgg tacagccaaa aaagacaaac
    22081 tgatagaaca acttgaagta cttgagaaag gcgaaaagga aaaaagtgac accctgcctg
    22141 gaaaatgacc ttaataagaa agctcgctga agcattcaga accaaatcgt acataacgat
    22201 taatttagtt ctggcattta tcatagctat tgtatttctg tattctgctg ttttttctcc
    22261 gcagaaaaac aattaccctg tgacgtgtat acatgaaaag attactggca aaccctgtgt
    22321 ctcgtgcgga ctttcacaca gcttttcact gataataagg ggcaaaataa ttgaagcaca
    22381 cagctggaat ccctacggtt ttcgtgtttt tctttttttt ctggcacagc tgctgatgag
    22441 agttgtattt tcggttttat accttaaaga agcagaatac aggaaacagc ttgttattta
    22501 tgatattacc ggcacaatta tattgtttct tattgccttt tacccgtttc tggcttatat
    22561 tttttcatca cttatacagg cctgatttta ttcctcttct tcaagaatcc gatatattag
    22621 atcaggctca aaacctctag agatagcaaa cctcaccagc ttgccccgga gttcaaagga
    22681 gttttttgcc ctcaccgatt ttctgtgtgt ttctataagc tttcttagag tatttatgta
    22741 ttcctcttca tcaattgagc caacagcctg ctgaattgta tcctcatcaa taccttttga
    22801 ttttagcatc acagcaatct tgaccttgcc ccaccggtta tgtttgaatt tatcccttgc
    22861 gtaagccgtt gagaacctta tttcgtcgat gaacttatct ttttgcagtg cttttagtat
    22921 tttttcacgt tcggcaggct gaatccccca tttttcaagc ctgctctcaa tctcacttct
    22981 gcaaaattcc ctgccagcac aaagcttcat ggctttttta agtgcttcgt tgtattttat
    23041 gttttcagcc attttctggt tgtatttttt tttatctcat ttaccggtta taaatctctc
    23101 ttttccgttc aggtccttga taattctggt ttctttgatt ccatatgaaa tcatgagtgt
    23161 attaagagct ttccccattt tttcatgcat ttcgaaatag atttttcctt ccggggcaag
    23221 aagaaaattt ttcttttcaa gaatggctct gtagtaaata agagggttat cgtcgggaac
    23281 aaaaagtgct gtttctggtt caaattctct aacattgatg tgtaaatttt tcttttccga
    23341 ttctggcaca tagggtggat tactgacaag aatattgaat ttttcgggaa agtattcagg
    23401 tccggctttc aaaatatcag ctttgaaaaa gtttaccttc acattattga gttttgcatt
    23461 ttcacgggct gtctcgagag ctttatccga gatatcgata gctgttatgg aagcataagg
    23521 tagatgtttt gccagggcaa ttgcaattgt acccgggcct gtacctatat ctattatttt
    23581 ccctttaaaa ccagggtttt ctttgagaat cagatcaacc agctcttcag tttcctgacg
    23641 ggggattaag gtagcgggat tgacttttat cctgcaattg taaaaaaaag tttctcccag
    23701 tacatattgt accggacttc ccttttcaag attattgcat atttccagga gctctcttct
    23761 gcgggtgggt gaaatttcat attctggttt catcatctcc tgcaacctgt tgagattgaa
    23821 aattgtcatg ataataaggt cggcaattga atttatctca ctggcagggt agagatgatg
    23881 caaccttgta tggaggtaat gacggaaatc tttaattgtt ctgaaattat cattcatagt
    23941 tcaaatttac tattttaggc cttatgttgg cagaagctga tcataaatac atgcagaggt
    24001 gtattgaact ggctcttaat gcagaagggc tgacatatcc taatcccatg gtgggtgcgg
    24061 ttgttgtaca tgatgacgtt attctggggg agggttatca tgtttatgct ggtggtcctc
    24121 atgccgaagt ggttgcaatt aacgcggtaa aaaagcggga gcttttacct gagtcgactc
    24181 tatatgtatc gcttgagcca tgctgtcatt acggaaagac acctccgtgt acagatctga
    24241 ttatttcttc cggtattcca cgggttgtgg taggaacaat tgacaccagc actaaagttt
    24301 caggcagggg tgttgccata ctgcggaaag caggaaagga ggttgaagtg ggcgtgatgg
    24361 aaaatgaatg ccggtgggtt aacagaagat tttttacata tcatgaacga aagaggccat
    24421 atattacgtt aaaatgggca gagagtgctg atggattcat agatattgaa aggcccgaag
    24481 gtttatctac cgggccttac tggattaccg gaatggctga gagagttctg gttcataaat
    24541 ggagagcctc ggaagaagct atcctggtgg gcggggaaac aatcaggaaa gataatccac
    24601 gtctgaatgt aagatactgg aaaggtaaag atccagtaaa gattatattg agcagatcgg
    24661 gaaatattgg cagttacctt gctggtaaaa ggacagatgg cggaataatt gtttttaccg
    24721 gcaacagaga atttaagccc gatggtgccg aagttgtatt tcttgaagaa aatgagcctg
    24781 ccgcattaag gatttgtaaa accctttatg ataaaggcat acagtctctg tttatcgaag
    24841 ggggagcaag ggttcttcag cattttatat cagagggatt gtgggatgag gcacggatat
    24901 tcaggggaca aaaatatttc aggaatggaa ttaaggcacc cgttgcagga ggaagggtaa
    24961 tttcagagac actctttgaa tcgggtgtgc ttgaagtttt aagtaatgat tatttataat
    25021 tataatgggt tgttaaaatc tgtttaaatt cttaattttg aaagaaattg aaattcgaaa
    25081 cataaaaata ttaagagata tgaaggggaa aatattttat acggtactgg tggtgcttat
    25141 tgcaacatct gtttcatttg cacagagtgg aaagaaggcc tataaggctg ggaaccagtt
    25201 aatgaagagc aataagtacg aagatgctat agttcaattt accaatgcta tcggagctga
    25261 acccaccaac gctgcttatt attattcaag ggctacagta tatgaactca ctggaaagtt
    25321 aaatgaggca tatgcagact atgagaagtc cctgatattc aagcccaaaa atgttccttc
    25381 actggttggt atggggaggg tatgcaataa acttggtaaa tacgacgaag ctctggttta
    25441 tctcaaccgg gcaaaagcaa ttgagaaact caacagggag gtttatccgg aaaaagtact
    25501 ggcactcata ggtcttgaga agtatgacca ggcacttaaa gcagccgata cagcattaac
    25561 tataaaagag acggcgtata attattattt gcgaggaaag gtttatgtga agctgaataa
    25621 tgatgcaatg gctcagcgtg aacttgaaaa ggcaattgca aaggacaggg gatttattga
    25681 gccccgcctt gaacttgcag atctttattt gagaagcaac aaacgagcag atgctcttaa
    25741 tcaggtaaat gaattaattg ctgctgctcc acaggatgcc cggggttatg tcgtaagaag
    25801 caggatttat aaagccaatc ttgattatcc aaatgctata aatgatattt caaagacaat
    25861 tcttctggat cccaacaacc ctgagtatta tatgataaga ggccgttatt atcaggagtt
    25921 caatcagcac atgaatgcca ttgccgattt ctccaaatat atagcaatga ggcccgacga
    25981 tcccgaagct tattttgcca gggcaaaatc gtaccaggag atgatgaatt ttgaaaaggc
    26041 aatagaagat tataacaaaa taacaactct ttcagagttt gatgtaaggg caaggaagct
    26101 tctgaaagag gcgcaggata tgctctatga gcttaacagg gaacaggtgc ctcctgagat
    26161 agaaattact tcacctgttg tatcttccgg taatataatg gaaataaaag gcaatgcaac
    26221 atccataact gtaacaggta aaataaggga taaaagcaag atagacgaag ttttaataaa
    26281 caatcagaaa gttctgacag gtgaaaagaa aaacaatgag gccgaattta ttgcaacagt
    26341 tgatgtgaga gatgttgaca gaataactgt gcttgcccgg gatgaataca ataatgaaaa
    26401 ggtgattgaa ctgaatttga gaaggacaga aatcaatcca cctaaaattt caattattgc
    26461 accatacgca tctgaggatg accagattta tcttgacacc aattcaccga ggctagctat
    26521 agaagggaaa attactgatg aaagcagaat caaatctatt ttcatagaag gtattacggc
    26581 tgcttatgcc gtgaatgaac ttaatccaac ttttaccgcc acaatagata ttctgaacaa
    26641 aaacaaaatt actgtcatag ccgaagatat ttacgggaac gtacaggaga aagaattcag
    26701 gcttaacagg gaaggtgcag agctatccgc ttccaatcct atgggcaaaa catgggttgt
    26761 tttcattgag aattcaaaat atacatcttt tgcacctctt gacggcccgg ttaaggacat
    26821 caacatgatg acaagagctc tttcaaatta tcagattcat cagataattt acaagaaaga
    26881 tatgacaaaa gccgatatgg aaaggttttt ctcaatagag ttgagggatc tgataaagag
    26941 caatcaggta aagtcgcttc ttgtatggta tgccggacac ggtaaattta ttaatgatgt
    27001 cggatactgg atacctgttg acgcaagaag agatgatgag tttacatatt tcagtctgaa
    27061 cactttacgg gctgcaatgg aaacgtatat taattatctt actcatttgc ttgtagttac
    27121 agatgcctgc gaatcaggac caagctttta ccaggcaatg cgttccgaca ttaaaatcag
    27181 aagttgtgac gattataccg caacacagct gaaatcatca caggtgttct cttcagcagg
    27241 atatgagctt gcagttgatg attcacagtt tactagaacc tttgcaaata ctcttgcaaa
    27301 caaccccaag gcatgcatac cgattgagga aattgttatt aatgttacaa atgccgtagt
    27361 aaacaataac cagcagaagc caaagttcgg aaagataacc ggactcaggg atgaggacgg
    27421 tacattcttc tttattgcga aataataata aatgaggtat ttatcaatga tttcagcaaa
    27481 acaggccatc agattatttc tgatggctct ggtatttcat gcctgcttac tgcagtcata
    27541 tgcccaggtt gtttattttg aaaaatacgg agttgagcag gggataggat cgtcaaaggt
    27601 ttataccatt attcaggaca gaactgattt catctggctt ggaacagaaa gtggcgtttc
    27661 cagatttgac gggaacaggt tcgaaaactt cggaagtgct cagggtcttg ctgccggagg
    27721 agtaagaagt atatttcagg atacaaccgg aagaatctgg tttggccacc tgaacggagg
    27781 gataagtttt tatgacggga gcaggttcag aattgcacgg attaattctt cttcagtgag
    27841 cggagatatt acaggagtaa ttcagacagg ctcatggtta tggtttgcca cttcggctag
    27901 cggagcgtac agaataagat ttccagaagc agcagatact gtattgcatg gaaaacatta
    27961 tgctggaaga gaaggtttga gcgatcaggt tttcagcatt tatattgata gaaataacaa
    28021 ccttttttgt atcaccgatg catttataaa aaaatataat gctgaacagg acacctttct
    28081 tacatattcc cctaaaggat tgacaactta tttcaacgtg gtggcgatgt ttcaggattc
    28141 aaaaggcaat tactggtatg gacttcataa tggcggactt tacaggcagg atgccgctac
    28201 tggggagatg aagatatttg atatcctgaa cggactttcc agaatgttta tttcatgctt
    28261 tgccgaagat tacaggggta atatctgggt aggaacatgg ggtggaggca ttacggtttt
    28321 tacaggagat aagaccacaa tatataataa aggtaacggg ctcaatgctc tgaatattca
    28381 ttcaattttc gaggacaggg aaaagaatat tcttattgcc gatcattatg aaggactgag
    28441 tatttacaag ggggatcatt ttattacata caatacacca gatattcttc ctgataacaa
    28501 tgtatgggct atctcacgcg atgaaacggg cagatactgg tttggtacca ataacggaat
    28561 atctgttttt gacccaataa agaagggcaa agataagata cagatttaca atgaaaaaac
    28621 agtaatgata gggaataata tcaggtttat tattccggat ttaggcagga tgtggattgg
    28681 tacttacggt aatggtgttt acaggtttga tctcaagcag gggaggttta tattcgatac
    28741 cgacctcaac aggatgcttc cacccgaccg cattgttacg ggcatggtaa ttgataaaaa
    28801 caaaaatctc tggataggga caaacgatgg tctggcaagg tggaatcctt caacctccga
    28861 aggttatgta tttacccaga tcaaaggtct ggcaggaaat attatcacat ctctcttttg
    28921 cgacagcaaa ggagtaatct gggcagggtc ggaaatgaag accggactgt cgaagcttct
    28981 gccaggtgct gataaattta tagttgtaga tatcggggaa gggattattc cacaaactat
    29041 aggggaaacg cccgacggaa caatatggat aggaactgtt aatggtctta ttgcactgaa
    29101 aaatgaaaag gtggagaaga catttacaga aaatgacgga ttgctttcaa ataacataaa
    29161 gtttttactt tctgac
//
LOCUS       DAOSNB010000003        27318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_1761_length_27318_cov_5.260206, whole genome shotgun sequence.
ACCESSION   DAOSNB010000003 DAOSNB010000000
VERSION     DAOSNB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27318
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1761_length_27318_cov_5.260206"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..782)
                     /locus_tag="PLG42_00340"
     CDS             complement(111..782)
                     /locus_tag="PLG42_00340"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOU29686.1"
                     /translation="MDLYALIVAGGSGKRMGIDTPKQFLELAGKPVLMHTIERFRKFS
                     SSIEIIAVLPENQLRFWNELLKKYSFDIPHTLVKGGKHRFISVKNGLEFVNGPGLVAI
                     HDGVRPLVSSETLKRCFEAAEKYGNAIPVISPSESIRIVTDEGSEPINRAKIKIVQTP
                     QVFDIDAIKNAYKQDYDPFFLDDATVLEKTGVKINLVEGNRENIKITNPEDLFIAQTL
                     LPFVS"
     gene            complement(804..2453)
                     /locus_tag="PLG42_00345"
     CDS             complement(804..2453)
                     /locus_tag="PLG42_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU29687.1"
                     /translation="MKERLIDYIEQSIRRNWNIEALSNYREKGYTYREIAEKILKLHI
                     FFRRAGIAEGDKIALVGRNSANWCVVYLAVVTYGGVIVPVLPDFKPEDMTNIINHSDS
                     KLLFVDDKIWENLDFSRVPHITSVISVDTFNLLVSRDPSLETSYRETDNEFRKFYPDF
                     KPEDVKFSDVTNDKLAVISYTSGTTGFSKGVMLPHNSLAANVRFAQHHMPLDPGDPVV
                     SFLPLAHTYGCAFEFLFPFTYGCHITILAKTPSPQIILQAFGEIKPRLILSVPLVIEK
                     IYKKQILPVISKPHMKLLLSIPGINKLLYKKIREKLEHSFGGRFKELVIGGAAFNPDT
                     EKFFRKIGFRFTVGYGMTECGPLISYASWDTTELGASGRPVDTLEVKIDSPNPENQVG
                     EILVRGENVMAGYYKNEEATKAVIDSEGWLHTGDLGVMDKKGNIYIKGRCKSMILGPS
                     GKNIYPEEIESVFNNEKYIAEAIVIDEANRLVALVYPDYDQLKSDNIPEDKIPEVFDD
                     IRKRVNDRLPDYMAVSKVRIQKEEFAKTPKRSIKRFLYAKE"
     gene            complement(2506..2892)
                     /locus_tag="PLG42_00350"
     CDS             complement(2506..2892)
                     /locus_tag="PLG42_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29688.1"
                     /translation="MANFPSILSYILGAVFIILIFSLAYAYLKPHILHRTRPVSTLLL
                     KVSYLVYVLVLLIVVYLAALVRGGLDKVFFGIEFFAFLIVLFVPTMGIFARKLQQFRK
                     QRENYNYFFTAVNLLSIFALLIMYIF"
     gene            complement(2922..3896)
                     /locus_tag="PLG42_00355"
     CDS             complement(2922..3896)
                     /locus_tag="PLG42_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU29689.1"
                     /translation="MAGKKVAAGIDIGGTNSVFGLVDREGNILAGGSVKTAHYPDAEE
                     FTKVVYNEVIKALDSLGGKAELAGFGIGAPMGNINRGTIEYPAGLPWKGITPLADFFR
                     KYTSLPIIVTNDANAAAVGEMIYGAAKGMKDFVVLTLGTGLGSGFVVNGRLVYGNDGF
                     AGELGHTTIKPGYSDRECGCGKKGCTETYVSATGLKRTLLKVMANTLQPSRLRRYSFE
                     ELDAKIIYEAALEGDPLALKAFEYTGMMLGIKLADVVAHTSPEAIFLFGGLANAKEFI
                     FEPARKYMEENLLSIYRGKVKLLPSGLEEKNAAVLGASSLVWSELENK"
     gene            complement(4133..4795)
                     /locus_tag="PLG42_00360"
     CDS             complement(4133..4795)
                     /locus_tag="PLG42_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOU29690.1"
                     /translation="MRFSKILEVRKEFFPEKCPDPLEVSSRMNFEGSGHFIDEVNWKE
                     FPYKPEVEFDIRYSERAIFLKYRVAENYFKAEKTSHNDRVWEDSCVEFFIEPAADGIY
                     YNLEFNAIGTCYIGAGRNREERQPLPESVISKLRVFPSSGNKPVREVEGRISWELTII
                     IPFGVFCYHTIESFEGKEIRANFYKCGDKLKVPHYLSWNPVGTLMPDFHRPEYFGILR
                     FI"
     gene            complement(4782..5735)
                     /locus_tag="PLG42_00365"
     CDS             complement(4782..5735)
                     /locus_tag="PLG42_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU29691.1"
                     /translation="MKEVVTGIDIGGTNTVMGLVDRDGNILAEASLLTTSYPEVQGFV
                     DAIAGAVSDMRKKAGDVNIRGIGIGAPNGNYYKGTIEQATNLVWRGIIPIADMIEKKT
                     SVKVVLTNDANAAAMGEMIFGAAKGMKDFIVLTLGTGLGSGIVVNGQMVYGHTGFAGE
                     LGHMVIVPGGRACGCGSHGCLETYASATGLVRTVLLLLSEMREETPLRKVSPAELTSK
                     MVAEAAAKGDPVALKAFDYTADKLALGIANSVIFSSPQAVFLFGGLVNAGELLFKPLR
                     EHINKYVMPWYRGRFEVLPSGIKEHNAAVLGAAALAWNEIL"
     gene            complement(5837..7624)
                     /locus_tag="PLG42_00370"
     CDS             complement(5837..7624)
                     /locus_tag="PLG42_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOU29692.1"
                     /translation="MKKALLFTSAGVFAIVVYLASCAKMVAPSGGPKDVDPPVVVKSL
                     PPQNSIFFKGKSIQITFNEYITLDKLNEKFMISPPMDRKPEISLRGKTLKIEFSEELK
                     DSVTYTLYFQDAIRDLNEGNPLVNFQFVFSTGGYIDSLSVTGNVFMSYSLNPSKNVLV
                     MLHSELADSAPRKRIPDYVTIASETGFFRINNVKPGKYRLYALSDNNNNKLYDLQDEA
                     FAFYDHVIDVSPESNYLSPGEDSLMNGSRVDSLKRPIPVEGDYQLYLFTAPVKKYYLT
                     SSTRSMQYKLVYTLSRPPDSLAFDFIADEKDKFLIEKNATGDTMTVWILESLLWSQQE
                     IRTLIKYPFTDSTDNLVYRTDTIPMRYFAVKPVRGKEKREILSITTNISGGFLKPGQN
                     IMLASAIPLLEPDTSGIKLYLAEKDKRTVIPYQLFADTTDRKRYILKAKLAEEMRYQL
                     ITTRGAFRSIYGIVSDSAAFTFTVRPESSFGHLTVILNNGEGKMIVQLLDKSEKVIAE
                     QKAEANERVLFPFLEKGTYRLRVIYDFNGDGRWNTGDYDLKLQPEPVSFYPDEIDVKT
                     DWRIEQEWDVGRRNEKSWKLRDIKQQQTR"
     gene            7779..8594
                     /gene="lgt"
                     /locus_tag="PLG42_00375"
     CDS             7779..8594
                     /gene="lgt"
                     /locus_tag="PLG42_00375"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOU29693.1"
                     /translation="MQLLIIPWDPDPEIFRIGSFAIRWYGLLFASSFLFGYIIMKKFF
                     ANEKVNEDILDRLTVYIALGTIIGARLGHCLFYEPSYYLRNPVEILKIWHGGLASHGG
                     AIGILIALWLFERKEKKGYFWALDRIAIVTALAGFFIRMGNLMNSEIYGVETTLPWGF
                     VFLRNNEVVPKHPTQIYEGLSYLIIFFILYRWYWKKKGNIYQGLLISVFLILVFSARF
                     MIEFVKEPQVEFEKSMILNMGQILSIPFILAGICGIFLSLKYKVPGVKPVQKK"
     gene            complement(8595..9179)
                     /locus_tag="PLG42_00380"
     CDS             complement(8595..9179)
                     /locus_tag="PLG42_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU29694.1"
                     /translation="MVNKEEIRKRIISTARDIFGRYGFRKTTMEEIARELKMGKSSIY
                     YYFKSKEEIFEAVVRYEANLLRSELTRAIKAVDSPVDKMKNYVRVRMKAFEKLSNYYN
                     AIFDKNLDHFDFIEKIRAKYDLEELAILRLILYDGNRKNVFRVKNSEYMAMAIQTAMK
                     GFEVPLFWKKKEIDVEDRVNAVLDMLFYGILRKE"
     gene            9713..10819
                     /gene="mtnA"
                     /locus_tag="PLG42_00385"
     CDS             9713..10819
                     /gene="mtnA"
                     /locus_tag="PLG42_00385"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOU29695.1"
                     /translation="MRIEGKSYRSVWIEENKPEVVKIIDQGKLPFSFEIKELTSVEDV
                     YNAISEMAVRGAPAIGVAGAYGMYLATLEITVHTRIHEHLRNAASYLSSARPTAINLS
                     WAVSRVLKNIEECESRTEIISKALKSAQEICEEEIKKCIEIGNYGFEIIKNISLKKNG
                     DSVNILTHCNAGWLACVDYGTATAPVYTARDKGIPVHVWVDETRPRYQGARITCFELE
                     NEDIPHTLITDSTGGYLMQKGMVDIVITGSDRVTRCGDCANKTGTYHIALAAKDNNVP
                     FYIAVPSSSFDLNISDGLKEIPIEERSPEEITETEGINGNSINKVRICNYSTRAVNYS
                     FDITPARLITGLITDKGVIKAEENNIVKLFKKNS"
     gene            10820..11416
                     /locus_tag="PLG42_00390"
     CDS             10820..11416
                     /locus_tag="PLG42_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOU29696.1"
                     /translation="MNGLVKFNCHWTQTGPVISDEQFEIINYWREVLYNMDLIGAYEN
                     GVGFGNISMRTGPGNQFIITGSSTGEIPELEPEHYVKVVSFNIDENAVQCIGPLKASS
                     ESLTHAAIYMSDPDINAVVHVHDIDLWNELIHKAPTTNPGMDYGTMGLAKDILRLFKE
                     PEVYEKRIIIMAGDRAGILTFGHDMDEAINVLMQHLKK"
     gene            11419..12165
                     /gene="mtnP"
                     /locus_tag="PLG42_00395"
     CDS             11419..12165
                     /gene="mtnP"
                     /locus_tag="PLG42_00395"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOU29697.1"
                     /translation="MAKVGIIGGSGLENPEILKSQQEINIVNLYGAPSSPLICGTIED
                     TEVVIISRHGKQHTIPPSKVNNRANILALKEAGCTHIIATTACGSLREEIKRGDLVIP
                     DQFIDFTRHRIITFFDEFEPGKLGHTPMADPFDSRLRELLINTAKGLKLPIHEKGTVI
                     TIEGPRFSTRAESRMFRLWGADIINMSTAPEAILANEAGIPYAVIAMSTDYDSWKNDE
                     KPVTWEDVLEVFRQNVQNVLKLLIRVIPEI"
     gene            12235..12308
                     /locus_tag="PLG42_00400"
     tRNA            12235..12308
                     /locus_tag="PLG42_00400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12269..12271,aa:Met,seq:cat)
     gene            complement(12751..13716)
                     /locus_tag="PLG42_00405"
     CDS             complement(12751..13716)
                     /locus_tag="PLG42_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU29698.1"
                     /translation="MKNIIKAEVVLLFLCLTGVAVAQERNDVIKVYNEGAKAIQTDIN
                     AAIQAFENVIVLSEKVGESVNDLKQKAMQVLPGLYTKVAAAAYNEKKPASEVIAAAKK
                     AMAAAEKYNNATQKENARNILLRGYYQMGTELFAQKNYADALLVFDSILTINPDYVTA
                     IYNKALIYAAQNSSDDFEKTIDLFLQKVKAQNDEEKIKQASELALGYFRAAGSQENQA
                     GNLDEALSLLDKAARYGDDKDLFYFYADVYNKKKNFDKAAEFARKGLELETGDAEAKA
                     KYYFQLGLAQSGKGQVSEACASFKNAAYGVFVQAANAERKNLKCQ"
     gene            14062..15426
                     /locus_tag="PLG42_00410"
     CDS             14062..15426
                     /locus_tag="PLG42_00410"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOU29699.1"
                     /translation="MKISSLFLLFVLFLFSSCSEKRLTYPEAVINGDSILIENIFPYQ
                     TQHCHASTIVELPDHDLLVAWFQGSGERTAEDVVIMGARYNRKTGKWSEPFLMADTDG
                     FPDINPVLFIDNQSRLWLIWYTVMAYQWESSLLKYRISTEYMQKEGPPVWSWQDILIV
                     KADGSAPEGINSNDQFVKTLERKYREYHDYLVSAGYLSPDGKGFITEELWNVSLQRYM
                     DIAKGVDFIADGMEINEKGEKIRTRLGYPLRRRIGWQTRNKPLITGGRILLPLYSDGF
                     DFSLIAISDNNGNSWQFSEPIVGAGSIQPALALCNDSSITAFMRDNGPQPKRLMISRS
                     FDRGATWSTVQDTEIPNPGSAADIVVLKSGKWVLVSNDLESGRHRLTVKLSEDEGKTW
                     PYSKSIVNGEPGSQTRAHYPAIIQGHDELIHITFTNQIPADGGKTNVKNIAHAVFSEK
                     WLMN"
     gene            complement(15440..17404)
                     /locus_tag="PLG42_00415"
     CDS             complement(15440..17404)
                     /locus_tag="PLG42_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU29700.1"
                     /translation="MKKGRAGRFSAENQGKWYKNTTLIAILFVTVVLTLTAYRRAFDN
                     EFVDWDDFTYVVNNNLVRSQADTVLDDIFSIPVSSNYHPLTILSLRLNNNSCQTCQNE
                     ISPAPFIMWNIILHLANTVLVFLLVYRLSGKKVFASFFVAVVFGVHPLHVESVAWISE
                     RKDVLYSFFFLSGLLSYLAFKDATSSIYKALFYILTFILFVFSCLSKAVAVVFPLVLV
                     LIDYWTEPHFDRANILQVIKKWTDLRRILLILPFLVVSLFTGLLAYHLQNGENFLGLM
                     RIDKDLPDVVNTVRKLTVFQRIGIGCAGLVSYLIKFFLPFKLSAIYPYPPEEVMKSAG
                     FIAGMAVSTLITIAIAVTSVAFMKKTKILPFGTAFFFITIMLVLQFISVGIAIKADRY
                     TYIPYIGLAFIPAMFIEEKLRERTRKTIVIISCFFVVFLWFLTSRQADVWQNTETLWT
                     DVIKKYPEAETPRASRGKFYTRKSLSAKSVSEKTYFEEKALADFMVAVKVRTKNTDVY
                     EGIGYILASRGDYKKALEYFNLAIIADQQNGSAYYNRALVYTSLNMPEEAIKDYTTAL
                     RYSPEKKREIINNRSNLLLETGRFREVIPDLDYLISLEPTNPNHYFNRATARLVTGDI
                     QGAAADLRQVLRLNPADREALELLKKIQSG"
     gene            complement(17415..18176)
                     /locus_tag="PLG42_00420"
     CDS             complement(17415..18176)
                     /locus_tag="PLG42_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU29701.1"
                     /translation="MINGKKIAVVLPAYNAEKTLKQTYEEIPGDIVDFIILTDDGSSD
                     RTLELARKLSIDHIIRHEVNRGYGANQKTCYDRALELGSDIVVMLHPDYQYTPKLIPA
                     MCHLIASGLYDAVLGSRILSKGAVKGGMPLYKYIANRILTFFQNILLNQKLSEYHTGY
                     RAFSAELLRKIKYHNNSDGYIFDNELLAQIIYLKARIGEISCPVRYLPESSSIDFFRS
                     IIYGLGVICVSLKFVLQKTGLFNFKLFSFQESAES"
     gene            complement(18228..19112)
                     /locus_tag="PLG42_00425"
     CDS             complement(18228..19112)
                     /locus_tag="PLG42_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOU29702.1"
                     /translation="MKKTLFLLALISLAVTARGQQATKGDIPFKYEELTSPQFVKAVE
                     KAGGVCVIPIGVFEKHGPHLPLGTDLFEAREVAFTAAKKEYAVVFPPYYGSQIFEAKH
                     QPGTIAYSSELIWKMLEETCNELARNGLKKIILLNGHGGNTNFLQFFCQSQLMKNNGY
                     IVVLFRPGEVQSLAGEIKSLKKAKLDGHAGEEETSMMYFIRPDLVDTESMTKESGLDQ
                     GRLKQLKYGYTGIWWYASFPNHYAGDVVQANKRLGELLVEQDAVQLAELIKFLKSDNS
                     IERLQEEFFKRAENPMAK"
     gene            complement(19150..19440)
                     /locus_tag="PLG42_00430"
     CDS             complement(19150..19440)
                     /locus_tag="PLG42_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOU29703.1"
                     /translation="MQFLLIAFDGKEPGAEDLRARVRPVHLEKIAELKRKGEFLFGGA
                     ILDDEGRMKGSMIVYELPDRQSLEERLKDEPYLYEGVWQKVEIYPFRLARID"
     gene            19638..20432
                     /locus_tag="PLG42_00435"
     CDS             19638..20432
                     /locus_tag="PLG42_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOU29704.1"
                     /translation="MAENKFLYKTGLVLSGGGARGFAHVGVLKALNEAGIFPDAVAGT
                     SAGAIAGVLYCDGYTPDEIMNIMRSRSWLEYMRPSVSKTGLLSISGVQKILKEHLRAK
                     TFDELKIKLYVTATDFNNGKAVYFEKGDLIKTVIASASIPVIFKPVIIEGITYFDGGV
                     LDNLPIQPLEGKCRVIIGSYVNPTGPEKNFNSLIKIAERTFLLNLSREVNSKATRFDL
                     LIAPTELKNYPVLDPEKSEEIFRIGYETTRELLKSKEIYNKLLFKK"
     gene            complement(20438..21280)
                     /gene="nfo"
                     /locus_tag="PLG42_00440"
     CDS             complement(20438..21280)
                     /gene="nfo"
                     /locus_tag="PLG42_00440"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOU29705.1"
                     /translation="MKYVGAHVSAEGGVHNAPLNAHRIGAKAFALFTKNQRQWFAPPL
                     SEEVIKLFRQNCETLGYKSFQILAHDSYLINLGHSEKEALEKSRKSFLEEFKRCELLG
                     LDRLNFHPGSHLNLTGEEDCLKIIAESINITLEKTRGVTAVIENTAGQGTNVGYKFEQ
                     IRYIIELIEDKTRVGVCFDTCHAYAAGYNIKDAKGFMETFKLFDEIIGINFLRGLHIN
                     DSKKEYASRVDRHENLGKGFLGEEVFKLIMNDSRFDNIPIILETPDESLWEEEIRKLY
                     SYVK"
     gene            complement(21306..22709)
                     /locus_tag="PLG42_00445"
     CDS             complement(21306..22709)
                     /locus_tag="PLG42_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU29706.1"
                     /translation="MSGFFGAVSKSSCVEDVYYGTDYHSHLGTRRAGMVFYEPGKGFR
                     REIHSIENAYFRNKFENALSMFEGNAGMGIISDTDPQPLLFNSHLGPFAIATVSRIVN
                     ISELEKYFLEKKIHFTEHFEGNINPTEIVANLICEKDDFVSGIDNVFEKVKGSCSLLI
                     LTSEGIIAARDRLGRTPIVIGKKEGAYAVATESSSFANTGYKIVYFPGPSEIVLVKED
                     GYTQLKKPSDEMQICAFLWVYYGYPPSYYEGINVEECRYRCGAALAKRDVIEADFVCG
                     IPDSGIGHAVGYSNQKGLPYKRAYSKYTPTWPRSFMPQDQSQRDLVARMKLIPNDEII
                     KGKRIIFLDDSIVRGTQLKESAKELRLCGAKEVHMRIACPPLMFPCTFLNFSQSRSIY
                     ELAGRKAIRQLGEDSKDITEYTDPDSPKYKKMVSCITENLCLDSLMYQRLDDLVKAIG
                     LPKEKLCTHCWDGSSYF"
     gene            22824..23723
                     /locus_tag="PLG42_00450"
     CDS             22824..23723
                     /locus_tag="PLG42_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29707.1"
                     /translation="MSDSNKTTKNFSQNKPPVFLIFITVILSIAFIFLVISYFSQKNK
                     MIEMETVLTHEKDSLANEIRKIMYGYDTLRSNNDSLNAQLARERERIRRLLSINASNV
                     ELIKKYRAEINTLREIMKSYIVQIDSLNTRNKLLAAENQDIRQKFSQVQKTNIELEKV
                     KEELSTKVEIASVIQAKNIVAVALNKNRKETTRIDRLDKLRVCFTLRENPIATAGKKT
                     VYMRIIRPDQLLITTSPDNLFDYGNTKMIYTESRSVDYINQDVDMCIYVDNTGDFIPG
                     NYNGELYLEGNLIGTFTFMLTKR"
     gene            complement(23743..24870)
                     /gene="hemW"
                     /locus_tag="PLG42_00455"
     CDS             complement(23743..24870)
                     /gene="hemW"
                     /locus_tag="PLG42_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOU29708.1"
                     /translation="MAGIYIHIPFCKSICHYCDFYKVLDEDNHSRFINAVLLEAKLRK
                     DYLSGESVSTIYLGGGTPSVLSVKELSDLIDGLYGIFNVENDCEITIEVNPDDVGIEW
                     LNEIRKIGFNRLSYGIQSWNDDILRMLNRRHNSKQAAEALIFSEKAGFENISVDLIYG
                     IPGYDSASWGKDLEQTFGYNIKHLSAYHMTIKPGTFFGKLKKAGKLNELDEEESNNQF
                     NLLIEKAENAGFIQYEISNFAKEGWFSKHNSNYWRQVIYIGLGPSAHSFNRISRQWNI
                     SNLEKYISAVESNKKFYTREELDNKKIFNEYILTSLRTMWGIDLDYIEINFSKEGYDY
                     VMNLSRKFIDYGLMRLENNNLVLTNTGKMISDNIISEFMMV"
     gene            24923..25312
                     /locus_tag="PLG42_00460"
     CDS             24923..25312
                     /locus_tag="PLG42_00460"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOU29709.1"
                     /translation="MFTFMKFFRNNILSILFSALIAVSSFTSSKSISKINFLHLQNID
                     KVVHLLMYACLTFLILFENRNRIKNKIHYVLIISITFAYGLIIEILQPIITNSRSREP
                     ADMMFNLSGIIAVVIIWYVINNYKKKL"
     gene            complement(25307..25747)
                     /locus_tag="PLG42_00465"
     CDS             complement(25307..25747)
                     /locus_tag="PLG42_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOU29710.1"
                     /translation="MISNTCKYGIRALVYLASKTDGSGSINIKTISRDLNLPNPFLAK
                     ILQQLVRNKILYSSKGPHGGFSFAKDPCKISIYEIISIIDGNELFENCVIHNRTCKSA
                     DAEKLLCPIHAKFSNIRNEIVKLFRETTISHLVESAKQSNEIML"
     gene            complement(25749..26189)
                     /gene="rpiB"
                     /locus_tag="PLG42_00470"
     CDS             complement(25749..26189)
                     /gene="rpiB"
                     /locus_tag="PLG42_00470"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOU29711.1"
                     /translation="MKKIGIASDHAGYFLKGKIIEFLKKNGYEFKDFGSFSDESTDYP
                     DYAHPLALSISKGEYDLGITICATGNGINMTVNKHQKIRSALCWNEEISRLARAHNDA
                     NICALPGRFISEPEAILIVRTFLTTEFEGGRHTNRIAKIPLKIV"
     gene            26658..>27318
                     /gene="nadA"
                     /locus_tag="PLG42_00475"
     CDS             26658..>27318
                     /gene="nadA"
                     /locus_tag="PLG42_00475"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010182653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOU29712.1"
                     /translation="MTKIDYIKEIERLKKEKNAIILAHFYQTPDIQDIADYVGDSLAL
                     AQKASSADASIILIAGVKFMAETAKILVPEKKVLIPDINAGCSLADSCTPEEFKKFIE
                     ENPGRTVVSYVNTSVEIKAMSDIICTSSNALQIIESLPENEKIIFAPDRNLGNYIKSK
                     TTRDIIVWDGACHVHEKFSIEGILELKKKYKNARIIVHPECELPVRMIADFIGSTSAL
                     LK"
BASE COUNT         8485 a         5720 c         4650 g         8463 t
ORIGIN      
        1 tgataaaaca acttttcgca tattcaatta ttttaacaat gcaatataca aattttaaaa
       61 tcagaagccc gttacctata aaaattgaaa tttcaataaa aggtatttct ttatgaaacg
      121 aatggaagaa gcgtctgtgc aataaagagg tcttcaggat tggtaatttt tatgttttcc
      181 ctgtttcctt ccaccaggtt tatcttcacc cctgttttct ccagaacggt tgcatcatca
      241 agaaagaagg gatcataatc ctgtttatag gcatttttga tcgcgtcaat atcaaaaacc
      301 tgcggtgtct gtactatttt tatttttgcc ctgtttattg gttcacttcc ttcgtcggtt
      361 acaatacgga tcgactccga aggacttata acaggaatag cattaccata tttttctgca
      421 gcttcaaaac aacgtttcaa agtctcactg ctcacaagtg gcctcactcc gtcgtgaatt
      481 gcaaccagac ccggtccatt tacaaactca agtccgttct taactgaaat aaatctgtgc
      541 tttccccctt ttacaagagt atgaggtatg tcgaaagaat attttttcag caactcattc
      601 cagaacctga gctggttttc gggaagaact gcaattattt caatcgaact gctgaatttc
      661 cggaaacgtt caattgtatg catcagaaca ggttttccgg caagctcaag aaactgcttg
      721 ggagtatcaa tgcccattct tttgccgctt ccaccggcaa ctattaaagc atataaatcc
      781 ataccaacaa tttcttccag ttattattct ttcgcataaa ggaatctctt aatacttctc
      841 ttcggtgtct tggcaaattc ttccttctgt atccttacct tgcttactgc catatagtca
      901 ggaagccggt cgttaactct ttttcttata tcatcaaaaa cttcaggaat tttatcttca
      961 ggaatattgt cactcttcag ctggtcataa tccggataaa caagagctac aagcctgttg
     1021 gcctcatcaa ttacaatggc ttcagcaata tatttctcgt tattaaatac cgattcaatc
     1081 tcttcgggat aaatattttt gcctgaagga cccagaatca tacttttgca tcttcctttt
     1141 atatagatat ttcctttttt atccattaca ccaaggtcac cggtatgaag ccagccttca
     1201 ctgtcaataa cagcctttgt ggcttcctca tttttataat aacctgccat aacattttca
     1261 ccccttacaa gaatttcacc cacctggttt tcagggttgg gtgaatcaat ctttacctcc
     1321 agtgtgtcaa ccggtctgcc cgaagctcca agctctgtgg tatcccacga ggcgtaactg
     1381 atcagcggcc cacactccgt cataccatat ccgacagtaa acctgaaacc tatttttctg
     1441 aagaatttct cagtatccgg attgaatgct gccccaccta tcacaagttc tttaaatctt
     1501 ccgccgaatg aatgttcaag tttctccctt atctttttgt acaagagctt atttattccc
     1561 gggatcgaaa gcaaaagctt catatgaggc ttgctgataa ccggcaaaat ttgcttcttg
     1621 taaatctttt caattacaag aggcaccgat agtatcagcc ttggtttaat ttctccaaaa
     1681 gcctgcagta ttatctgcgg tgaaggcgtc tttgcaagaa ttgttatatg acacccgtat
     1741 gtgaaaggga aaaggaactc aaaggcacaa ccgtaagtat gtgctagcgg cagaaatgaa
     1801 accacaggat cacccgggtc aaggggcata tgatgctggg caaaccttac gtttgcagcc
     1861 agactgttgt gcggcaacat cacacccttt gaaaatcctg ttgtccctga agtataactt
     1921 ataactgcaa gtttgtcatt tgttacatca gaaaacttta cgtcttcagg tttaaaatca
     1981 ggatagaatt tcctgaattc gttgtctgtc tctctgtaag aagtttccag agaaggatcc
     2041 cttgaaacca gaagattgaa agtatcaaca gaaatgacag atgtaatatg cggtactctg
     2101 gaaaagtcaa gattttccca tattttatcg tcaacgaata aaagttttga atcactgtgg
     2161 ttaataatat tggtcatatc ttccggttta aaatcgggca ataccggcac aattactcca
     2221 ccatacgtta ctacagcaag gtaaacaaca caccagttgg cagagtttcg tccaacaagg
     2281 gcaattttat ccccttcggc tattcctgct cttctgaaaa atatatgcag tttcagaatt
     2341 ttttctgcaa tttcccggta agtgtaaccc ttttccctgt aattcgacaa agcttcaatg
     2401 ttccagttcc tgcgaatgct ttgttcgata tagtctataa gtcgttcctt tatcatattt
     2461 tccctgttta aattcgttac taaattctat ataaatctaa gaaaatcaga aaatatacat
     2521 tattaggagt gcaaaaatag aaagcaggtt cacggcagta aaaaaatagt tatagttctc
     2581 cctctgtttt ctgaattgct gaagtttcct tgcaaatatg cccattgtag gaacaaaaag
     2641 cactatcagg aaagcaaaaa attctatccc gaaaaatact ttatctagac ctcctcttac
     2701 aagtgcagca agatatacaa caatcaatag aacaagtaca taaacaagat agctcacttt
     2761 aagcagaagc gttgaaaccg gacgtgttct atgcaaaata tgcggtttca gatacgcata
     2821 tgcaagcgaa aatataagaa tgataaaaac tgctccaaga atgtaactca gaatactggg
     2881 gaaattagcc atatctctgg ttttttagta ctatatgaaa attatttgtt ttcaagttcg
     2941 gaccatacca gtgaacttgc cccaagtact gcagcattct tctcttcgag accagaggga
     3001 agcagcttaa cttttcccct gtaaatgcta agtagatttt cttccatata ttttctggca
     3061 ggttcaaaaa taaactcctt cgcatttgcc agtcctccga aaaggaatat tgcctcagga
     3121 ctggtgtgtg ctacaacatc agcaagttta ataccaagca tcataccagt atattcgaaa
     3181 gcctttaatg ccagcgggtc tccttcaagt gcagcctcat aaataatttt cgcatcaagt
     3241 tcttcgaagc tgtatctcct tagcctgctt ggctgaaggg tatttgccat aactttcagc
     3301 agtgttcttt tcaatcctgt tgccgaaaca tatgtttccg tacaaccttt tttcccacac
     3361 ccgcattctc tgtcggaata gccaggcttg atagtagtat gacccaattc acctgcaaat
     3421 ccatcattgc cataaaccag tctgccattt acaacaaaac cgcttccaag accggtgccg
     3481 agagtaagta ctacaaaatc cttcattcct tttgcggctc cgtaaatcat ctcccctaca
     3541 gccgcagcat ttgcatcgtt ggtaactata attggaagtg aagtatattt tctgaagaaa
     3601 tctgcaagag gcgttattcc cttccatggt agtccggcag gatattcaat agtaccccgg
     3661 ttgatattac ccattggagc acctattccg aatccggcaa gctccgcttt acctccgaga
     3721 ctatccaatg cctttataac ttcattatac acaactttcg taaattcttc agcatcagga
     3781 taatgtgcgg ttttaacaga gcctccggca agaatgtttc cttccctgtc tacaagacca
     3841 aaaacactat tagtacctcc tatatcgatt cctgcggcta ctttttttcc agccatcaag
     3901 cagaaaaatt ggtttaagtt caggatacaa atatcaataa aataatgcaa ttaaaatatt
     3961 acaccttatt tttttatcat aagaatctaa acctaacctt atgaatatta tcgaatatgt
     4021 ttatattacg gtttatttat ttaccgattt ctgctggcat gttaacagac aatttaagga
     4081 ctattatatc actctttaca ttcgtcattc aacctgttac cagcactact gattaaatga
     4141 atctcaggat tccaaaatac tcaggtcggt gaaaatcagg cataagtgta cctacaggat
     4201 tccacgaaag ataatgcggt acttttaatt tatcgccgca tttataaaaa tttgctctta
     4261 tctccttgcc ctcaaaagat tcaattgtat gatagcagaa aactccaaaa ggaattatta
     4321 tagtaagctc ccaggatatt ctgccttcca cttctctcac aggtttattt cctgatgaag
     4381 gaaatactct caattttgaa attactgatt caggcagagg ctgcctctcc tccctgtttc
     4441 ttcctgcacc tatatagcag gttccgattg cattgaattc cagattataa tatatcccgt
     4501 cggcagcagg ctcaatgaaa aactctacac atgaatcttc ccataccctg tcattatgag
     4561 atgtcttttc agctttaaaa taattttcag ctaccctgta tttaagaaaa attgcccttt
     4621 cactatacct tatatcaaat tctacttcag gtttatatgg aaactctttc cagttcacct
     4681 catcaataaa atgtccggaa ccctcgaagt tcatccttga tgaaacttct aagggatccg
     4741 gacacttttc cggaaagaat tcttttctta cttcaagtat tttagagaat ctcattccat
     4801 gcgagtgcgg cggcacccag aacagcagca ttgtgttctt ttatacctga aggcagaacc
     4861 tcaaacctgc ctctgtacca gggcataaca tacttgttta tatgttccct gagaggtttg
     4921 aacagaagtt ctcctgcatt caccagtccg ccaaaaagga aaactgcctg tggacttgaa
     4981 aagataactg agttggcaat accgagtgcc agtttgtctg ctgtatagtc gaaagctttc
     5041 agtgcaacgg gatctccctt ggcagctgcc tctgccacca tcttcgatgt gagttctgct
     5101 ggtgatactt ttctcagtgg cgtttcttcc ctcatttcgc taagcagaag cagtactgtt
     5161 cttaccagac ctgttgctga tgcataggtt tcaaggcaac catgacttcc acagccgcat
     5221 gctcttccac ctggcacaat aaccatatga cccagctcac ctgcaaaacc ggtgtggcca
     5281 taaaccatct gaccgtttac aacaatacca ctgccgagac ctgttccaag tgtcagaaca
     5341 atgaaatctt tcatgccctt tgcagcgccg aaaatcatct cacccattgc agcagcattc
     5401 gcatcattgg tcagaacaac ctttactgat gtcttctttt ctatcatatc tgcaatggga
     5461 ataatgcccc gccagacaag atttgttgcc tgctcaatgg tacccttgta ataattacca
     5521 ttcggtgcac cgatacctat gcccctgata ttcacatccc cggccttctt tcgcatgtcg
     5581 ctaacagccc cggcaatggc gtcgacaaac ccctgtacct ccggatatga ggtagtaaga
     5641 agactggctt cagccagaat atttccatct ctgtcaacca gacccatcac tgtgttggtt
     5701 ccacctatgt caatacctgt tacaacttct ttcataattt aagatttaaa agaattaaac
     5761 attaacttgt tttatctgtg tctcaaatat aataagtcct gatttaaaaa aataccaatt
     5821 aagttttttt agcatgttac cttgtctgct gctgctttat gtctctcaac ttccaggatt
     5881 tttcatttct cctcccaaca tcccactcct gttcaatacg ccagtcggtc ttaacatcaa
     5941 tttcgtcagg ataaaacgaa actggttcgg gctgcagttt taaatcataa tcgcctgtat
     6001 tccatctgcc gtcaccatta aaatcgtata ttaccctcaa acggtaggtc cctttttcaa
     6061 gaaaagggaa aagaaccctt tcgtttgctt cagccttctg ttcagcaata actttttcac
     6121 tcttatcaag cagttgaact atcatcttac cttcaccgtt attcagtatc actgtaagat
     6181 gcccgaaaga gctctcaggc ctcacagtaa aagtaaaagc tgccgaatca gaaactattc
     6241 cataaatact cctgaacgct ccccgggtgg taataagctg gtatctcatt tcttcggcaa
     6301 gttttgcctt cagaatatat ctcttacgat ctgtagtatc ggcaaaaagc tgataaggta
     6361 tcacagtcct tttgtctttt tcagcaagat aaagttttat tccagatgta tccggctcaa
     6421 gcaaaggaat tgctgaagcc aacattatat tttgccccgg cttaagaaaa cctcctgaaa
     6481 tattagtggt gatactcaat atctcccttt tttcttttcc cctcactggc ttaaccgcaa
     6541 aatacctcat gggtattgtg tccgtacggt aaactaaatt atcagtagaa tctgtaaaag
     6601 gatactttat taaagttctt atttcctgtt gcgaccacag caggctttca agaatccata
     6661 cagtcatagt atctcctgtc gcattctttt caatcagaaa tttatctttt tcatctgcaa
     6721 taaaatcaaa tgccagagaa tccggaggcc ttgagagagt ataaaccaat ttgtactgca
     6781 tactgcgggt agaagaggta aggtaatatt tttttacagg agctgtgaaa agataaagct
     6841 ggtaatcgcc ttcaacaggt atggggcgct tcagcgaatc aaccctgctc ccgttcatta
     6901 aactgtcctc ccccggtgag agataattgc tttcaggact tacgtcaata acatggtcgt
     6961 aaaatgcaaa agcttcatcc tgaagatcat aaagtttgtt gttattattg tctgaaagtg
     7021 catataacct gtattttccg ggttttacat tattaatcct gaagaaccct gtttcactgg
     7081 caatagtaac ataatcgggt atccttttcc gcggagcaga atcggcaagt tcactgtgaa
     7141 gcatcaccag cacattttta gaaggattaa gagaatacga cataaacaca ttgcctgtta
     7201 ccgaaaggga atcgatataa cctccggttg aaaaaacaaa ctgaaaatta accagcggat
     7261 taccctcatt aaggtccctg atcgcatcct gaaaataaag cgtatatgta acactgtctt
     7321 tcagctcttc ggaaaactca atttttaatg ttttacccct gagtgaaatt tcaggttttc
     7381 tgtccatcgg aggggaaatc ataaattttt cattcagctt gtcaagggta atatattcat
     7441 taaaggttat ctggatactt ttgcctttga aaaaaatgct gttctgtgga ggcagacttt
     7501 ttacaactac aggaggatca acgtctttcg gcccgcccga aggagctacc atcttcgcac
     7561 atgatgcaag gtaaactaca attgcgaaaa ctcctgctga cgtaaacaac aaagcctttt
     7621 tcatgaatct gaataacagg atacaaacat acaccaaatt tttattcttt taagccagtg
     7681 ctgactctgg agcataatta taaaatttaa tacacacctg attccggata gcggaattaa
     7741 atattttcta cctttgaaac cgtttaaaac ctgtcggtat gcaattgctt attattccct
     7801 gggaccctga tcctgaaata tttcgtatcg gttcatttgc tattagatgg tacggattac
     7861 tctttgcttc ttcttttctg tttggttata taataatgaa aaagttcttt gcgaatgaga
     7921 aggttaatga ggatatactc gaccggctta cagtatatat tgcactgggc acaataatag
     7981 gagcccgtct gggccattgc ctcttctacg agccttcata ctatctccgc aatcccgttg
     8041 aaattcttaa aatttggcac ggtggacttg ccagtcatgg cggtgcaata ggtatactta
     8101 ttgcattatg gcttttcgaa aggaaagaaa agaaaggcta tttctgggca ctcgacagaa
     8161 ttgcaattgt tactgcgctt gcaggcttct ttatccgcat gggaaacctg atgaactcag
     8221 aaatatatgg cgtagaaact actttgccat ggggttttgt tttcctgaga aacaatgaag
     8281 ttgttcctaa acatcccact cagatttatg agggtctatc ttaccttatt attttcttta
     8341 ttctctacag atggtactgg aagaagaaag gtaatattta ccagggactg ctgataagcg
     8401 tcttcctcat cctcgtgttc tcagcaagat tcatgataga atttgttaaa gaaccacagg
     8461 tggaattcga aaaatcaatg attcttaaca tgggacagat cctcagtata ccgtttatcc
     8521 ttgcaggaat ctgcggaatt ttcctgagtc tgaaatataa agtacccggc gtaaagcctg
     8581 ttcagaaaaa gtaatcactc tttccttaat atcccataga aaagcatatc aagaacagca
     8641 ttaacgcggt cttcaacatc aatttccttc tttttccaga aaaggggtac ttcaaaacct
     8701 ttcatcgcag tctggattgc cattgccata tattcactat tcttgaccct gaagacattt
     8761 tttctgttgc catcataaag tatgagcctt aaaatggcca gttcttcaag gtcatacttt
     8821 gcccttattt tctcgataaa atcaaagtga tccagattct tgtcaaatat tgcgttataa
     8881 taattggata gtttttcgaa tgctttcatt cttactctta cataattctt catcttatca
     8941 acaggagaat ccaccgcttt tattgccctt gtaagctcgc ttcttaacaa atttgcttca
     9001 tatctgacaa cagcctcgaa aatctcttcc ttgcttttaa aataataata aatggaactt
     9061 ttacccattt tcaattcacg ggcaatctct tccattgtcg tcttacggaa tccatacctg
     9121 ccaaaaatat cccttgcagt tgaaatgatt ctttttctta tttcttcttt attaaccatt
     9181 tattatttta tcaagcctat atacaattct atacaaatat agtaaactat tgtatgataa
     9241 aattttttag aatattctgg tcgattattc taatattatc agctttattt ccttaaaagt
     9301 tcaattccaa aagaataaat tctttatcaa cttttttcag gacgctaaag aaacgggaaa
     9361 ggggttggat gataagcccc ctctttacga agtagagagg gggttggggg tgagtacatt
     9421 ttttaagctt cccccctggc gtctgtctga ctggcgtcag ccgggcaggg aaacaagaaa
     9481 ggggatcaat taatcattga aacgggaaag gaggttatta ctccataaag aaacaggaaa
     9541 atgtataatt attttttaaa cttctttcag gacgacacac ctctgttggt taataaagtt
     9601 ttctgccatt ttacatttta cattaaaaat taaccagtat aatttaaatg cgatacttaa
     9661 ctattttata attttgtaaa ttaattcttc aggtaaccgg atattcatat aaatgagaat
     9721 agaaggcaag agttaccgca gtgtctggat tgaagaaaac aaacctgaag ttgtaaaaat
     9781 tatcgatcag gggaaactgc cgttttcttt tgagatcaag gaacttacat cggttgaaga
     9841 tgtgtacaat gctatttcag aaatggcagt aagaggggct cctgcaatag gtgtagcagg
     9901 ggcttacggc atgtaccttg caacacttga aattacggtt cacacaagga tacatgaaca
     9961 tcttcgaaat gctgcttcat acctgagttc tgcacggcca acagcaataa atctttcatg
    10021 ggccgttagt agggtgttga aaaatattga ggaatgtgaa tcacgcactg aaattataag
    10081 taaggctttg aaatctgcac aggaaatctg tgaagaagaa ataaaaaaat gcattgaaat
    10141 agggaattac ggatttgaaa taataaagaa tatcagtctg aaaaaaaatg gggactctgt
    10201 aaacatactt acacattgca atgcgggttg gcttgcctgt gtcgattatg gcactgccac
    10261 agcacctgtc tatactgcaa gagataaagg tataccggtt catgtgtggg ttgatgaaac
    10321 acgcccaaga taccaggggg cacgaataac ctgctttgaa ctcgaaaatg aagatatccc
    10381 tcatacactc attaccgaca gtacaggtgg ctacctgatg caaaaaggga tggttgatat
    10441 tgttatcaca ggtagtgaca gagtgacacg ctgcggagat tgtgccaata aaaccggaac
    10501 ataccacatc gctcttgctg caaaagacaa taatgtacct ttttacatcg cagtgccatc
    10561 ctcatcattc gacctgaata tttcagacgg attgaaagaa atccccattg aagaaagatc
    10621 tccggaagaa ataacagaaa cagaaggaat taacggtaat tccattaata aagttagaat
    10681 atgcaattac tctacccggg cagttaatta ttcatttgac ataactcctg cccgcctgat
    10741 tacaggatta attacagata aaggagtcat aaaggcagaa gaaaacaata ttgtgaaact
    10801 atttaagaaa aacagttaaa tgaacggatt ggtcaagttt aattgccact ggacacagac
    10861 aggtccggtg atatcggacg aacagttcga aattatcaat tactggcgtg aagttcttta
    10921 taatatggac cttatcggtg cttatgaaaa tggcgttgga tttggaaaca taagcatgag
    10981 aacgggtcca ggtaaccagt ttatcattac gggctcctcc acgggtgaga taccagaact
    11041 ggaaccagaa cattatgtta aagtggtttc attcaatatt gatgaaaatg cagttcaatg
    11101 cattggtcct cttaaagcct cttccgaatc gcttacccac gctgccatct acatgtcaga
    11161 tcccgatatt aatgctgtag tacatgttca cgacatcgac ctctggaatg aactgatcca
    11221 caaggctcca accactaacc ctggaatgga ttacggaaca atgggacttg caaaagatat
    11281 actgaggctt ttcaaggaac cagaggtata tgaaaaaaga ataataatta tggctggcga
    11341 ccgtgcagga attctcactt tcggtcacga catggacgaa gcaataaatg ttcttatgca
    11401 acatctcaag aaatagtaat ggcaaaagtt ggaattatag gcggttcagg tctggaaaat
    11461 cctgaaattc tgaaatctca acaggaaatt aatatagtta atctttacgg agctcccagt
    11521 tcgcctctca tatgcggaac tatcgaagat acagaagtgg tgataatatc ccgtcacggc
    11581 aaacagcata ccattccacc ttcaaaggtc aataacaggg caaatattct tgcccttaaa
    11641 gaagctggct gtacccacat tattgcaact acagcatgcg gaagcctcag ggaggaaatc
    11701 aaaagaggtg accttgtaat tcccgaccag tttattgatt ttacacgcca ccgaatcatt
    11761 actttttttg atgaatttga acctggcaaa ctgggccata cacccatggc agaccctttc
    11821 gactcccgcc tgcgtgaatt gcttataaat actgcaaaag gtctgaagct ccccattcat
    11881 gaaaaaggca cagtaataac aattgaaggc ccccggttct ctacccgggc agaatccaga
    11941 atgttccgcc tgtggggtgc agatataatc aatatgtcaa ctgcacccga ggcaatactg
    12001 gcaaatgaag caggaatccc atatgccgtg atcgcaatga gtacagacta cgactcatgg
    12061 aaaaacgatg aaaaacctgt tacatgggaa gatgtactgg aagttttcag acaaaacgtt
    12121 cagaatgttc ttaaattgct gattagagta atacctgaaa tttaaaaaac caatatgttt
    12181 tctttacaat ttgttttatt tgcaacaaat ttataatatt gcagtccgtt ttgagggccc
    12241 atagctcagc aggttagagc atctgactca taatcagggg gtcgctggtt cgaatccagc
    12301 tgggcccacc tccgccctcc gaagccttgg cgcagaaggg cttcatcatt ttatatggct
    12361 ttacacacgc caaacctttc tcaggcttca gcggacgagg cacacttaaa aatagctaaa
    12421 gcgagcattt cacttttttc taacctgctt tttgtcttaa aaagttacag aattataacc
    12481 atttctctct ctatacaaat ctgagaaaac ctttatttta cttttcttaa tccataatca
    12541 ggggtcgctg gttccccacc cgactgatgc aatacagaca cgcctttagg gtggaaatac
    12601 aaaaaaggcc tttacgttaa ttggtatttt attaaactgt catccttgtt tcaggacaag
    12661 acatctgtat agattactat cagagttgcc tgagattttt tcctgaccaa attaaaataa
    12721 aaatctggta taacttaaaa ccatataata ttactggcac ttgagatttt ttctttcagc
    12781 attggctgcc tgaacaaaaa ctccgtatgc tgcattctta aatgaagcac aggcttcaga
    12841 tacttgacct ttgcccgact gtgcaagtcc taactggaaa taatattttg ccttggcttc
    12901 tgcatcaccg gtttcgagtt ccagaccctt tctggcaaat tcagctgctt tgtcaaaatt
    12961 tttcttttta ttataaacat cagcataaaa atagaaaagg tctttgtcat caccatatct
    13021 ggcagcctta tcgagtaatg aaagtgcctc atcgagattt cctgcctgat tttcttgtga
    13081 tccagcagca cggaaataac ccagagctag ctccgatgcc tgcttaatct tctcctcatc
    13141 attctgtgcc tttaccttct gcagaaataa atctattgtc ttttcaaaat catctgaact
    13201 gttctgagca gcgtaaatca aagccttgtt atatatcgcg gtaacataat ccggattaat
    13261 tgtaagaata ctgtcgaata ccagcagggc atcggcataa tttttttgtg caaagagttc
    13321 cgttcccatc tgataatatc ctctgagcaa aatattccga gcattttcct tctgggtcgc
    13381 attgttgtac ttttctgcag cagccattgc tttctttgct gcagctatta cctcagaagc
    13441 cggtttcttt tcattatatg cggcagcagc aaccttcgta tacaaacctg gtaacacctg
    13501 catcgctttt tgcttcaggt cattaaccga ctctccaact ttttctgata acacaataac
    13561 attctcaaaa gcttgaattg cagcatttat atctgtctgt atggccttgg caccttcatt
    13621 ataaaccttt atcacatcat tgcgttcctg ggcaactgcc actcctgtta aacataagaa
    13681 taataaaact acttctgctt taataatatt cttcataaaa attaaaattt tagttaatta
    13741 attttcaata tttttactgt ggtaaattta taaataaaat taaattggca aaacttgtta
    13801 tacaatcaat attgccggct ttttaactgc cagatattaa tctttgcatt ttaaaatcat
    13861 tttttctgtt gacaaatata tttaaattct taataattta ttaatctgca ttttatcaac
    13921 aattgaaaaa cttcgaaaag aagactctct ccatatgaaa aattttaagt gtagctcgat
    13981 tttatatgaa attatacggt ttatttattg gtctttattc ttaattttac tgaactaact
    14041 attaataata ctttaattat catgaaaata tcctctttat tcctgctttt tgtactgttt
    14101 ctttttagtt catgttctga aaaaagatta acctatcctg aggcagtaat taacggagat
    14161 tctattctta ttgaaaatat tttcccgtac cagactcagc attgtcatgc ctcaacaatt
    14221 gttgaacttc cagatcatga cctccttgtt gcatggttcc agggaagtgg tgaacgaact
    14281 gcagaagatg ttgtaataat gggtgcgcgt tacaaccgta aaacaggaaa atggtcggaa
    14341 cctttcttga tggccgatac agatggcttt cctgatatta acccagtact tttcattgat
    14401 aaccaatcac gcctgtggct gatatggtac acagtgatgg catatcagtg ggaatcatct
    14461 cttttgaaat atcgcatcag cacagaatat atgcagaaag aaggaccacc tgtgtggtcg
    14521 tggcaggata tactcattgt taaagccgat ggctctgcac ccgaaggtat aaacagcaac
    14581 gaccagtttg taaagacact cgaaagaaaa tacagagaat accacgatta tctggtatca
    14641 gcaggttatc tttcaccgga cggaaaaggt ttcattaccg aagaactctg gaatgtttcc
    14701 ctgcaaagat atatggatat tgcaaaaggt gttgacttta ttgccgacgg tatggaaatt
    14761 aacgaaaagg gagaaaagat acgaacccga ctggggtatc ctctcaggcg ccgtatagga
    14821 tggcagacca gaaacaaacc tctcatcaca ggaggccgga ttcttctgcc cctctattct
    14881 gacggatttg atttttcttt aatcgctatt tctgataaca acggcaattc atggcaattc
    14941 agcgaaccca ttgttggagc cggttcaatt cagcctgctc ttgccctgtg caatgatagt
    15001 tctattacag catttatgcg cgacaacgga cctcagccca aaagattgat gataagcagg
    15061 tcgttcgaca gaggtgcaac atggagcact gtgcaggata cagaaatacc caaccccggc
    15121 tctgctgctg acatagttgt acttaaaagc ggaaaatggg ttctggtatc taacgacctt
    15181 gaatcgggcc ggcatcgact taccgtaaaa ctatctgaag atgaaggcaa aacatggcca
    15241 tattcaaaaa gtatcgtaaa cggcgaaccc ggaagccaga caagagcaca ctatcccgca
    15301 attatccagg gacatgatga gcttattcac atcacattta ccaaccagat accagccgat
    15361 ggcggtaaaa caaacgtaaa aaatatagcc cacgctgtct tttcagaaaa gtggctcatg
    15421 aattaagatc tatcatgaac taccccgact gaattttttt aagcagctcg agagcttccc
    15481 tgtcggccgg attcaggcgc agaacctgtc tgaggtcagc tgccgccccc tgaatatctc
    15541 ctgttactag acgggctgtg gctctgttga aataatgatt tggattcgtc ggttccagcg
    15601 aaataagata atcaaggtct gggataactt ctctaaaacg gcctgtttca agcaaaagat
    15661 tcgaacgatt atttattatc tctcttttct tttcaggact gtatcgaaga gccgttgtgt
    15721 aatcttttat tgcttcctca ggcatattca gtgaagtata taccagtgcc ctgttgtaat
    15781 aagcactacc attctgctga tctgctataa tggcaagatt aaaatattca agtgcctttt
    15841 tgtaatcacc ccttgaagcc agaatataac caataccttc ataaacatcg gtgtttttgg
    15901 ttctaacctt gacagcaacc ataaaatcgg ctaatgcctt ttcttcaaaa tatgtttttt
    15961 ctgatacact tttagcactc agcgatttcc ttgtgtaaaa cttgccacgt gaagccctgg
    16021 gagtttcagc ctcaggatac ttctttataa cgtcagtcca caaagtttca gtattctgcc
    16081 aaacatcggc ctgccttgaa gtcagaaacc agagaaaaac tacaaaaaag caggaaataa
    16141 ttacgatcgt tttcctggtt ctctccctta atttttcttc aataaacatc gccggaatga
    16201 aagccagtcc gatatacgga atataagtat acctgtcggc tttaatagct ataccaaccg
    16261 aaatgaactg cagaacaagc attatagtaa tgaaaaagaa agctgtccca aaaggcaaaa
    16321 tttttgtttt cttcataaaa gcaacggacg tgaccgcaat ggcaatggta atcagtgtgg
    16381 atacagccat gccagcaata aatcctgcac ttttcattac ttcttcaggc ggataaggat
    16441 atattgctga aagtttaaaa ggcaaaaaga acttaattaa atacgaaaca agacctgcac
    16501 atcctatacc aattcgctga aatactgtaa gttttcgtac tgtatttaca acatcgggca
    16561 aatccttatc aatccgcatc agtccaagaa agttttctcc attctgcaga tgataagcaa
    16621 gcaggccagt aaatagtgaa actaccagaa aaggtaaaat cagtaggatt cttcgcaaat
    16681 cagtccattt ttttattacc tgcaggatat tcgctctatc aaaatgaggt tctgtccagt
    16741 aatcaataag tacaagcaca aggggaaata ccacagctac agcctttgat agacatgaaa
    16801 agacgaaaag tatgaaagtc agtatataaa acagagcttt gtatattgaa cttgtcgcat
    16861 ctttaaaagc aagatatgat agaagtccag aaagaaagaa aaacgaataa agaacgtcct
    16921 tcctttccga aatccaggct accgattcca cgtgaagagg atgaactccg aaaactaccg
    16981 ctacaaaaaa agatgcaaaa actttctttc cgctaagcct gtaaaccaga agaaaaacca
    17041 gaacagtgtt tgccagatgt agtataatat tccacattat gaacggagca ggtgaaattt
    17101 cgttctggca agtctgacag ctgttgttgt taagtcttag agataatatt gtaagcggat
    17161 gataattcga agatacagga atagaaaaga tatcatcaag aactgtatcg gcctggctcc
    17221 tgacaagatt gttgttcaca acatatgtaa agtcatccca gtcaacaaac tcattgtcaa
    17281 atgccctgcg gtaggccgtt aatgtaagca cgacagtaac aaaaagtatt gcaatcagag
    17341 ttgtattttt ataccacttc ccctgatttt ctgctgaaaa ccttccggct cttccttttt
    17401 tcactgcctg tgcattaact ttctgcactt tcttgaaaac tgaataactt aaaattgaat
    17461 aaacctgttt tctgcaaaac aaacttcaac gaaacacaaa tcacaccgag gccataaata
    17521 atacttctga aaaaatctat tgatgaactt tcgggtaaat agcgaaccgg acatgatatc
    17581 tcaccaatcc tggctttcag ataaattatt tgtgccagta attcattgtc aaagatatat
    17641 ccatcagaat tgttatgata ttttattttc cttaataatt ctgccgaaaa tgctctgtaa
    17701 ccggtgtgat attcagatag tttctggtta agaagaatat tttgaaaaaa cgtcaaaatt
    17761 ctgttggcaa tatacttata aagcggcata cctcctttaa cagctccttt gctcagaatt
    17821 ctggaaccca gtacagcatc atataagcct gatgcaatta gatggcacat ggccggtata
    17881 agttttgggg tatactgata atcgggatgc agcataacaa ctatatcaga gcccagttca
    17941 agagccctgt cataacaggt tttctgattt gcaccatacc ccctgtttac ctcatgcctg
    18001 ataatatgat ctattgaaag cttccttgcc agctccagag tcctgtcact gctgccgtca
    18061 tctgtaagaa ttataaaatc aacaatatcc ccgggtattt cctcatatgt ctgctttaaa
    18121 gtcttttctg cattgtacgc cggaagaaca acagctattt tcttcccgtt aatcatttta
    18181 tacccgggga tttttatata caaattcagt tctgttagtt tcgtagctta ttttgccata
    18241 ggattttccg ctcttttgaa aaactcttcc tgcaatcttt caatagaatt atcactcttc
    18301 agaaatttaa taagctctgc aagctgaaca gcatcctgct caacaagaag ttctcccagt
    18361 cgtttgtttg cctgaaccac gtcacctgca taatggttcg gaaaacttgc ataccaccat
    18421 attcctgtgt atccatattt cagctgtttc aagcgtccct gatcgagtcc actctcttta
    18481 gtcatcgact cagtatcaac aagatctgga cgtataaaat acatcatcga tgtctcttct
    18541 tcacctgcat ggccatcgag ttttgccttc ttcagtgatt ttatttcacc tgcaagtgac
    18601 tgtacttcac caggccggaa aagaacaaca atatatccat tatttttcat cagctgtgac
    18661 tggcagaaaa actgcaggaa atttgtatta cctccatggc cattaagaag tattatcttt
    18721 ttaagcccgt ttcttgccag ttcattacag gtctcttcaa gcattttcca tatcagctcg
    18781 ctgctatacg caatagttcc gggttgatgt tttgcctcaa aaatttgact tccataataa
    18841 ggaggaaaaa ccacagcata ctcctttttc gctgcagtga atgcaacttc ccttgcttca
    18901 aaaaggtctg ttcccagcgg caaatgaggt ccgtgctttt caaaaactcc tatgggaatc
    18961 acacacacac cccctgcctt ttctacagcc tttacaaatt gaggagatgt caattcctca
    19021 tacttaaatg gaatatcccc ttttgttgct tgctgccctc ttgcagttac tgccagagaa
    19081 attaatgcca gtaaaaaaag tgtctttttc atgttagtaa atatttaatg ttcaacataa
    19141 tatgtttctc taatctattc tggcaagccg gaaagggtaa atttccacct tctgccagac
    19201 cccctcatag agataaggtt catctttaag cctctcttca agagactgtc tgtcaggtaa
    19261 ctcatataca atcatcgaac ctttcatcct gccttcatca tccagtattg cacccccgaa
    19321 aagaaattcg ccttttcttt taagttctgc tattttttca agatgtaccg gccgtaccct
    19381 ggcccttaaa tcttcagctc cgggctcttt tccgtcaaaa gcaataagta aaaactgcat
    19441 cacaatactt ttaaaagtta ttcttctgcc aaaatataaa ttaattgcaa ataattgtaa
    19501 caaaattatt tttgctttca gaacatatcg tttaaaattt ttatttgtgt gtgagaatca
    19561 tttaaaataa tgatatttgt aatataattt attctttttg aatgactgaa aatcaataaa
    19621 gaattattct taaaatcatg gcagaaaata aatttttata taaaaccgga cttgttctaa
    19681 gcggcggtgg cgcaaggggc tttgcgcatg taggtgttct taaagcactt aatgaagctg
    19741 gtattttccc cgacgcagtt gcaggaacaa gtgccggggc tattgcaggc gttctttatt
    19801 gcgacggata tacgcccgac gaaataatga atataatgcg ttcacgcagc tggctcgaat
    19861 acatgcgccc ttcagtcagc aaaacaggcc tgctgtctat ctcaggagtg caaaaaattc
    19921 ttaaagaaca tctccgggcg aaaactttcg acgaacttaa aataaaactt tacgtcactg
    19981 caacagactt taataatgga aaggccgtct atttcgagaa aggagatctc attaaaactg
    20041 taatcgcatc tgcaagtata cctgtaatat tcaaacctgt aattattgaa ggaataacat
    20101 atttcgacgg gggtgttctc gacaatctgc caatccaacc actcgaagga aaatgcaggg
    20161 tgataattgg ttcatatgta aacccgaccg gccctgagaa aaactttaac agtcttatca
    20221 aaatagccga gagaacattt cttcttaacc tgtcgcgtga ggtaaactca aaagcaacaa
    20281 ggtttgacct tttaattgct ccgacagaac tcaaaaatta tccggttctt gatcctgaaa
    20341 aatcagaaga aatttttaga ataggatacg aaaccaccag ggagctcctg aaatcaaaag
    20401 agatttacaa caaactatta tttaaaaaat agtcttatca cttaacataa gaatagagtt
    20461 ttctgatctc ttcttcccag agtgattcat ctggtgtttc gagtataatc ggtatgttat
    20521 caaagcgtga atcattcata attaacttga aaacttcttc gccaagaaat ccttttccga
    20581 gattttcatg acggtcaaca cgagatgcat attccttctt tgaatcatta atatgtaacc
    20641 ctcttaaaaa attaattcct attatttcat caaaaagttt aaatgtttcc ataaatcctt
    20701 ttgcatcctt aatgttgtag ccggccgcat aagcatgaca ggtgtcaaaa cagactccta
    20761 ccctggtctt atcttcaata agttcaatta tatatctgat ctgttcaaat ttataaccaa
    20821 cgtttgtccc ctgccctgca gtgttttcaa tcacagcagt aactcccctg gttttttcca
    20881 gtgtaatatt tattgactct gcaataatct taagacaatc ttcttctcct gtaaggttca
    20941 gatggcttcc gggatgaaaa ttaagacgat caagaccgag tagttcgcat cgtttaaatt
    21001 cctctaaaaa cgacttcctc gatttttcaa gagcctcctt ttctgaatga cccagattta
    21061 ttagataact gtcatgagca agaatctgaa atgatttata acctaaagtc tcacagtttt
    21121 gtctgaacaa ctttatgact tcctctgaca gtggtggcgc aaaccattgt ctctgatttt
    21181 tggtaaataa agcaaatgct tttgcaccta ttctgtgtgc attcaaaggt gcattatgta
    21241 cacctccttc tgcactaaca tgtgcaccta catatttcat aatgaattac atttaaaagt
    21301 aaatattaaa aataactgct cccatcccag cagtgggtac atagtttttc ctttggcagt
    21361 cctatagctt tcaccagatc atccagcctc tggtacatca atgaatcaag gcatagattc
    21421 tcggtaatgc aggataccat cttcttatat ttcggagaat ccgggtcggt atattctgta
    21481 atatccttac tgtcttctcc gagctgcctt atcgcttttc tccctgcaag ttcatatata
    21541 gaacgagatt gggaaaaatt aagaaaggta cagggaaaca tcagtggagg acatgctatt
    21601 ctcatatgta cttcttttgc tccgcataag cggagctcct ttgcactttc tttcaattgt
    21661 gtgcctctta caatactatc atccagaaaa attattcttt ttccctttat tatttcgtca
    21721 ttcggtatca gtttcattcg ggctacaaga tccctctggc tctggtcctg tggcatgaaa
    21781 cttcttggcc atgtaggggt atactttgaa tatgctcttt tatacggtaa acctttctga
    21841 ttgctgtaac caactgcatg accaattccc gaatccggaa taccgcaaac aaaatccgct
    21901 tcaattacgt ctctcttagc aagagctgca ccacacctgt acctgcactc ttcaacattt
    21961 atcccttcat aatacgaagg tggataacca taataaaccc agagaaaagc acatatctgc
    22021 atctcatcag atggcttctt caactgtgta taaccatctt ccttcaccag aacaatctca
    22081 gaaggccctg gaaaatatac aattttatat cctgtattgg caaatgaact tgattctgtt
    22141 gctactgcat aagcaccctc ttttttacct atcacaatcg gtgttctccc aagacggtca
    22201 cgtgcagcaa ttatcccctc agaagtgaga atcaacagag aacacgaccc tttgaccttt
    22261 tcaaaaacat tatcaatgcc ggaaacaaaa tcatcctttt cgcaaatcag atttgcaaca
    22321 atttcagtag gattaatatt cccttcaaaa tgttctgtaa aatgtatttt tttttcgaga
    22381 aaatacttct caagctcact tatatttaca attctgctta ccgttgcaat tgcaaaaggg
    22441 ccaagatggg aattaaatag aagaggttgg ggatcggtat cacttataat gcccatccct
    22501 gcattacctt caaacattga gagtgcattt tcaaatttgt tgcggaaata tgcattttca
    22561 atgctgtgaa tttcacgcct gaaacctttt ccgggttcat aaaaaaccat ccccgctcgc
    22621 ctggttccaa gatgtgaatg ataatcggtt ccgtaataaa catcttcaac acaagatgat
    22681 tttgatactg caccaaaaaa tccactcata caaggatcat taactaattt aatatcaaaa
    22741 tgaaaaattt aacgctaatt aatttttaaa acatcataaa tataaaccga tattattatt
    22801 tttatcccct aaattaatta aacatgtctg attcaaataa aacaaccaaa aatttttcac
    22861 agaataaacc tccggttttt cttattttta ttacagttat cctctctatt gcttttattt
    22921 ttctggtaat aagctatttc tcacagaaaa ataaaatgat tgaaatggag actgtactca
    22981 cacatgaaaa agattccctg gccaatgaaa tacggaaaat aatgtacggg tatgataccc
    23041 tcaggtcaaa taatgattca cttaatgcac agcttgcccg ggaaagagaa cgtatacgcc
    23101 gcctcctgtc aataaatgcg tcaaatgtag aactcattaa aaagtaccgt gccgaaataa
    23161 ataccctcag agaaataatg aaaagctata tagtccagat agattcactt aatacccgta
    23221 ataaattact tgcagctgaa aatcaagata tcaggcaaaa attcagtcag gttcagaaaa
    23281 ccaatattga acttgaaaaa gttaaagaag aattatcaac aaaagtcgaa atagcttcag
    23341 ttatacaggc taaaaacata gtggcagttg ctctgaataa aaaccgcaaa gagaccacaa
    23401 gaattgatcg tctcgacaaa ctccgggtat gctttaccct cagggaaaat cccatcgcaa
    23461 ctgctggtaa aaagaccgta tatatgagaa tcataagacc cgatcagctg ttaattacta
    23521 caagtccaga caacctattc gattacggaa atacaaaaat gatttataca gaaagccgga
    23581 gtgtagacta catcaatcag gatgtcgata tgtgtatata tgtagacaat acgggagatt
    23641 ttattccggg taactataac ggcgaactct accttgaagg aaatctgatc ggtacattta
    23701 catttatgct gacaaaaaga taaagataat cagcaatagt tactatacca tcataaattc
    23761 agaaattata ttgtctgata tcatttttcc tgtattggta agaacgagat tattattttc
    23821 aagtctcatt aacccgtaat cgataaattt cctggataag ttcatcacgt aatcgtagcc
    23881 ttccttactg aaatttattt ctatatagtc aagatctatc ccccacatag ttctgaggga
    23941 agtcaaaatg tattcgttga atattttttt attgtccagt tcttctcttg tatagaattt
    24001 tttattgctc tcaaccgcag atatatattt ttcaagattg ctaatattcc actgtcttga
    24061 tatcctgttg aaagagtgtg ccgaaggacc cagcccgata tatattacct gcctccaata
    24121 gttactgtta tgcttggaaa accatccttc ctttgcaaaa tttgaaattt catattgaat
    24181 aaaaccggca ttttcagctt tttcaataag aaggttaaat tgattgttgc tttcctcttc
    24241 atccagttca tttagtttac cggctttttt aagcttcccg aagaatgttc ccggctttat
    24301 tgtcatatgg taagctgaaa gatgtttaat gttatatcca aaggtctgtt caagatcttt
    24361 tccccagctg gcagaatcat acccgggtat accatatatc aggtcaaccg aaatgttctc
    24421 aaacccggct ttttcagaaa aaataagagc ctccgcagcc tgtttactgt tgtgtcgacg
    24481 attaagcatc cttaaaatat catcattcca cgattgtatt ccataactca acctgttaaa
    24541 tcctattttc ctgatttcat tcaaccattc aattccaaca tcatccggat ttacttcaat
    24601 tgttatctca caatcatttt caacattgaa aattccatat aatccatcaa ttaaatcaga
    24661 taactcttta acactcagta ccgaaggtgt tccaccccct aaatagatcg ttgatacaga
    24721 ttcccctgag agataatctt ttcttaattt cgcttccaga agaacagcat taataaacct
    24781 gctgtgatta tcttcatcca gtaccttata aaaatcacag taatggcaaa tacttttaca
    24841 aaacggtatg tgtatgtata ttccggccat attacaatgt cctgcataaa attatgaaat
    24901 tatatgttac aaaatttact ttatatttac cttcatgaaa ttcttccgaa ataacatact
    24961 ttctattttg ttttcagctc tgattgctgt ttccagcttt accagttcca aaagcatcag
    25021 caaaatcaat tttctacatc tgcaaaatat cgacaaagtt gtacatcttc tgatgtatgc
    25081 ctgcctgact tttttaatcc tgtttgaaaa cagaaacagg attaaaaata aaatacatta
    25141 tgtcttaatt atttctataa cttttgccta tggcctgatc attgaaattc tccagccaat
    25201 aattacaaac agcagaagta gagaacctgc agatatgatg tttaacctct ccggtattat
    25261 agctgtggtg ataatatggt atgttataaa caactataaa aagaaattat aacattattt
    25321 cattggattg ttttgccgat tctacaagat gacttatagt agtttccctg aaaagcttaa
    25381 ctatttcatt tctgatattt gaaaattttg cgtgaatagg acatagaagt ttttctgcat
    25441 cagctgattt gcatgtacgg ttatgtatta cacaattttc aaataattca tttccatcta
    25501 taattgatat aatttcataa atactgattt tacatggatc ctttgcaaaa gaaaatcctc
    25561 catgaggccc ctttgacgaa tataaaattt tgtttcttac aagctgttga agtatttttg
    25621 ccagaaaagg attcggtaga ttaaggtccc tggaaatcgt tttaatatta atactacctg
    25681 atccatccgt tttacttgcc agataaacca gggctctgat tccatatttg catgtatttg
    25741 aaatcatgtt aaacaatctt taatggaatt tttgctattc tgttagtatg tcttccacct
    25801 tcaaattcag tggtaaggaa agtcctgaca atcaaaattg cttctggctc cgagataaac
    25861 ctgccaggca aagcgcatat attggcatca ttatgtgccc tggctaaacg actaatttct
    25921 tcattccagc aaagcgcact ccttatcttt tgatgtttgt ttactgtcat atttattcca
    25981 ttacctgtag cacaaatagt tattcccagg tcatattcac ctttcgaaat tgataaagca
    26041 agaggatgag cataatctgg ataatctgta ctttcatctg aaaaacttcc aaaatccttg
    26101 aattcatatc catttttttt cagaaattca attattttgc ccttcagaaa ataaccagca
    26161 tgatcactgg caatacctat ttttttcatt ttcattaatt tatttaatcc gaattataat
    26221 attcagataa agcgtaaaag taatcaccag atacaaataa atcaaagaga tgatcaaatc
    26281 aatattttat taacaacaat tttatttatc tgtttataaa tcattagaat ttattaagat
    26341 actatttttt gaaaatttaa taaaagacct taaacctctt ataaacaatt taacatttaa
    26401 ttttgtttat gtttttatga agatattaat aaattaacag atgttaatat aatgtttaaa
    26461 acgctgaatt tcaaaaacgg tataattttt gacatgttaa aaaaatgttt ttatgtatgt
    26521 gattgtaata tttaaaagca aattttatta tttatttcaa caatattaac agaatatatt
    26581 ctaattcttc taagaaaaat ttttaaacct gtaataaaga acattgttaa aaaccgatat
    26641 attaaacatc ccgggaaatg acaaaaatag attacataaa agaaattgaa agacttaaaa
    26701 aagaaaagaa cgcaataata cttgcgcact tttatcagac tccagatata caagatattg
    26761 ccgattacgt gggagacagt cttgctttag cacaaaaagc ttcgtcagct gatgcttcta
    26821 ttatattaat tgcaggagtt aaattcatgg ctgagactgc gaaaatattg gttcctgaaa
    26881 agaaagtttt gatacccgat ataaatgcag gatgctcgct agcagattca tgtacacctg
    26941 aagaatttaa aaaatttatt gaagaaaacc caggaagaac agtcgtttca tatgttaata
    27001 cttcagttga aataaaggca atgtctgata tcatatgtac gtcatcaaat gcgctacaga
    27061 taattgaatc tctacctgaa aatgaaaaga taatttttgc gcccgacaga aaccttggta
    27121 actatataaa aagtaaaacc acaagagata taattgtatg ggatggagcc tgtcatgttc
    27181 atgaaaaatt ttcaattgaa ggaatccttg aattaaagaa aaaatataaa aatgccagaa
    27241 taattgtcca tccagagtgc gaattaccgg taagaatgat tgctgatttt attggttcaa
    27301 cttcagcatt acttaaat
//
LOCUS       DAOSNB010000004        26025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_1881_length_26025_cov_5.170466, whole genome shotgun sequence.
ACCESSION   DAOSNB010000004 DAOSNB010000000
VERSION     DAOSNB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1881_length_26025_cov_5.170466"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..3464)
                     /locus_tag="PLG42_00480"
     CDS             complement(420..3464)
                     /locus_tag="PLG42_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU29713.1"
                     /translation="MKISELAVRNYQFTIIVFLMLVVMGIISFIKIPQAEDPEFPIPI
                     FPVIAVYPGASPVDIEQLVVDKVERSINELEDLKRIKTEIKDGVAVIVVEFTAESDPD
                     KKYDEILRQLNSLRPSLPEGLYSLEAIKIQAGNTNIVQSAIVSETASYETLGDYAEEL
                     RDLVSSVPGVRKAEALAYPAKEVRIEIDLSKMAQLKLTVSRIIAAIQSENANIPAGSI
                     DVGPKKFNIRTTGGYQSVENVRNTIVGSDNGQIVYLKDVAQVMWSNEDKKYFGRYNGE
                     KAAFVIASMKKGYNIHRVRNDIYKVYDSFEKKLPKGIRLERGFDQSQNVRRKLTRLEK
                     DFLFAFFLVIVTLLPLGFRASGTVLVSIPLSLMIGVTGLYFTGFSINQLSIVGGVIAL
                     GLLVDDSIVVIENISRWVREGKSPSEAAIGATRQIGTAVLGCTATLIFAFLPLMFLPG
                     MAGKYMRSLPTTIAFVVTGSLFVALTIIPFIASRMLKGNTDPRGNVVLRAFHKAINFT
                     YSKALHWSLNNPVKTLTGATILFTASLFLIKVIGFSLFPKAGIPQFLITVETPRGSNL
                     NETDRAVKFVESVLSGRPEVKYFMSNIGKGNPMIYYNSFQKSGQTTLGEVMCEVKPEY
                     LKQLQMFVEWLRDTLNTYPNGKIYVNEFENGMPVDAAIAVRLVGNNLDTLYKYAEVIE
                     EIFRSTPGTIYIKNPLSQSLTDLRIEVNSEKAGMLGIPSIEVDRTIRLALAGIEAGRF
                     RDSDGKEYPLMVRQVHRGEADIDRLNSIYIASLTGSLIPLSQIARVEFSDSPTVIQHY
                     NRERSITVSASVMTGYNTDRLTRQVLEKLYQLKLPDGYRIIPAGELESRRESFAGIWS
                     AILIAVFGIFTILLLEFKTFKGTLIVLSVIPLGIIGGILMLYITGNTLSFAAAVGFVA
                     LIGIEIKNSILLVDFTNQLREEGVSLDEAIQKAGEIRFLPVVLTTLTAVGGLMPIALS
                     GSALYSPLAYVIIGGLITSTLFARLVTPVMYKLIPPEIKTVK"
     gene            complement(3472..4503)
                     /locus_tag="PLG42_00485"
     CDS             complement(3472..4503)
                     /locus_tag="PLG42_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU29714.1"
                     /translation="MKKEILFLLILQFLVLSCKSRQEQHIVADLNNVKVVSVASSQES
                     LPVHAAGVIMPEEEIKLSFKTGGIVEHILVKEGDRVKKGTLLASLNLSEISSQAELAR
                     NAYEKALRDYTRVRNLYADSVATLEQLQNATTALNMAKSNLDIANFNLERSKIVAPAD
                     GIILKQLVRENELVAAGYPVFLFGLSGKMWKVTAGLADRDIIRVRQGDSAVVMPDAWP
                     GVRLPGIVEKTGELAGPLTGTFDVEIRFSDGGYRLAAGFIAGVDIFPSVSKNTVYVPA
                     GSVVDADGNTGYIFALTDSSTVKKIKVVITGIRGSEIAVENLPGDVTLVVSEGAAYLK
                     DGMKVNVLN"
     gene            complement(4500..5870)
                     /locus_tag="PLG42_00490"
     CDS             complement(4500..5870)
                     /locus_tag="PLG42_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU29715.1"
                     /translation="MKKYILFAAVLFSPFLCGQESLEEYIQYGLENNLVLKQKTLDCQ
                     KSLEALREARALFYPGISFIARYTRSEGGRTIEFPAGTLMNPVFMTLNALTHSNLFSP
                     IDDQVIKFLRPREHETKIRVTQPVFSPEIYYNTQIRKEINLYNEVDLNQYRRELIAEI
                     KKAYYNVAMASAMLDILKETGKLLEENVRVSRKLFENDKVTKDFVYRSESELARYEQE
                     LQRAENNRKTAVAYFNFLLNRPLETPVITSFPDSFPTISPVTEQYTYSAISGREELEK
                     LRKYQNMAELQVKMNQANKLPDIYFVFDYGFQGEEYRFNKEQDYLQASAVLTWTLFEG
                     YRNRAKIRQAMLDKAEADSKLEEAKKKIELEVLTTLSELFTSEKEILTAEKRLRNASE
                     GFRLVQRKYEEGQASLLEFLDARSNLTQAEENLVISKFNYLSCFADFERVTVINSDIY
                     EKFSIK"
     gene            complement(5883..6338)
                     /locus_tag="PLG42_00495"
     CDS             complement(5883..6338)
                     /locus_tag="PLG42_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOU29716.1"
                     /translation="MEKELQKLIDQFLRILHLYSLITRKPKDYGTGDMLYFTEVHTLA
                     MIGRNQGLNITRLAELMGVTKGAISQTIRKLSAKGLILRKQTKNKKEVLLNLTEKGLT
                     VYRAQESFRKELFAFAGSLYRQAKPEDAELVGRLFDAILKNMKERIDKR"
     gene            complement(6526..6981)
                     /locus_tag="PLG42_00500"
     CDS             complement(6526..6981)
                     /locus_tag="PLG42_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29717.1"
                     /translation="MNFSNFITSTGKRINRDHFIHLVQVAKLDGKISETEMEILHKEG
                     RKFGLTDPEIDDIINNEHPLNYHPPYSLEDKFFELYNITELILSDNVITEAEKKMIRR
                     YAIAAGFPDDVIEGLCKILIEGVKKGEEEEALLKEFRKKYISKYKQSRK"
     gene            7138..7827
                     /locus_tag="PLG42_00505"
     CDS             7138..7827
                     /locus_tag="PLG42_00505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU29718.1"
                     /translation="MKNPFLCRMWFIPLLFTLFLTPVANSQSLKENPALDEQVRKFLN
                     ENSRRWYDMNVPEVDGKLLYDIIIKNNYKSALEIGTSTGHSGIWIAWALSKTGGKLIT
                     IEIDEGRHRQAVENFKKAGLSEYIDARLADAHQLVKELKGPFDFIFSDADKEWYKNYF
                     IELDPKLKVGGCFTAHNVYDYGSGGRGSRGGFRSGQQEFLEYVHSLPNYETTVDNRGG
                     GVSISYKKSSR"
     gene            complement(7834..9042)
                     /locus_tag="PLG42_00510"
     CDS             complement(7834..9042)
                     /locus_tag="PLG42_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU29719.1"
                     /translation="MKTKLFASLFLLSIVFPVFAQQIKTASQKQTSTDEGYVFTTVAD
                     LKATSVKNQAITGTCWCFATTSFIESELLRMGRGEYDLSEMFVVRYNYIDKLKDNYLR
                     QGRGNLGEGSLAHDWMIVFSREGIVPEEVYSGLNYGSTIHNHSELNAFINAVAEVPVR
                     RKNESPQYHQIVDAIFDIYFGKVPETFTYQGKSYTPKSFAASLGINPEDYVEITSFTH
                     FPFYTRGVVEVPDNWRMEKFYNVPLDELIQIMDYSLTNGYTIAWDGDVSESGFMHSRG
                     IAILPATEGGDNYSTSMGRAENEGSQVIYTKTAGNRPVPEIKVTQEIRQAGYENFSTT
                     DDHMMHITGLLKDQYGTKYYKTKNSWGTERNPFGGYLNMSENYVRAKTIFIMVHKNGV
                     PPAIKAKLGI"
     gene            complement(9053..9418)
                     /locus_tag="PLG42_00515"
     CDS             complement(9053..9418)
                     /locus_tag="PLG42_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOU29720.1"
                     /translation="MADINNIGPAGEDIAVRHLVEKGYKIRHRNWKSGQKEIDIVAEN
                     ENYVVFVEVKTRKDDYYIHPRDAVTDKKQKMIIYAADSYIRIYNIDKESRFDVITVIS
                     DGKSFTVEHIEDAFYSTLR"
     gene            complement(9424..10065)
                     /locus_tag="PLG42_00520"
     CDS             complement(9424..10065)
                     /locus_tag="PLG42_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU29721.1"
                     /translation="MEKIKIIIVDDHHLFRNGLKLLLEGIPEFEITGEASNGAELLDI
                     IDRTRADIVLMDINMPVMDGVEATIKVLERKPEIKVIALSMYGEEDYYYRMTDAGARG
                     FILKDSDISEVREAINTVKRGGTFFSQQLLQNVIQKIKHREAETKVAVLSKREKEILE
                     KICEGLSNQEIANALFISKRTVDKHRANLLEKTNSKNTASLILYAIRNKLIKI"
     gene            complement(10077..11024)
                     /locus_tag="PLG42_00525"
     CDS             complement(10077..11024)
                     /locus_tag="PLG42_00525"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU29722.1"
                     /translation="MVRLIALIISIALQVIAAIIALSFVKMTKYRLSWILLSFSFLFM
                     AIRKIIQLWELLRGTPSFTWQMIDEWMGVFISFMIIVGVILIREIFYSLKRAETDRLK
                     TERRVLNAIINTEENEKKRFAKELHDGLGPLLSTVKMSLSALNGKITDPAGIDILQNT
                     NHVVNEAINTIKEISNNLSPHVLTNFGLSSAINAFTSKVNKTRALEIDFKSNMEGVRL
                     DNDKEVVIYRAVCELINNSLIHSGASRVEIELNKHDKFITLQFSDNGRGFDTSILGTE
                     DCKGMGLSNIDTRVKSIDGVFFIESSKGKGTNALIKIIV"
     gene            11155..12405
                     /locus_tag="PLG42_00530"
     CDS             11155..12405
                     /locus_tag="PLG42_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOU29723.1"
                     /translation="MNFLYSLAVRLYVWSIYAASPFNLKARLWVKGRKGWKEKLSSKS
                     EYLNNPVWIHCASLGEFEQGRPLIEMIKEKKPDQKILLTFFSPSGYEIRKNYSFADYI
                     CYLPSDTRKNAKYFIETVKPVMAIFIKYEFWNNYISELYLKGIPLYLVSGIFRNSQHF
                     FKWYGSFFLGMLKKFTHFFLQDEASAELLSKAGINNFTVTGDTRFDRVSKIAASAPVI
                     PVIEEFRNGEKVFLAGSSWPEDEKIIVKYINSNPGRMKWIFAPHEPEPSNIDRLERMI
                     KTNCVRFSEFSGKNRDARVLIIDKIGILSSAYRYAYIAAVGGGFGKGIHNILEPACWG
                     IPVMFGPNYRKFREALSLLGRKGAFCFRNFEEFSTILDELLSSMELYKSASEATLKYV
                     YENTGATDRIFSSIFQQDINNNQV"
     gene            12553..15855
                     /locus_tag="PLG42_00535"
     CDS             12553..15855
                     /locus_tag="PLG42_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOU29724.1"
                     /translation="MIKKTRVIFGVLMFLAFSAVSFGQGATTSSMAGKVTDNRGQALP
                     GATVVAVHVPSGTVYGASANAEGFFLIQGMRPGGPYKVEVSFVGYTKKTYTDITLFLG
                     ETFDLNASLTESSQELTEVVVVGDKPSAFNSLKTGVSMNIRSQDISSLPSISRSINDF
                     IRLSPLYGGSNSFAGRDGRYNNIVIDGSNFNNNFGLSSRNMPGGDAEPVSLDAIEEIQ
                     VNVAPFDVRQANFTGAGINAITRKGTNEYSASVYTYYRDQSFNGTKVGDFELPPAAKT
                     STKIIGARVGGPIIKDKLFFFINGEYESSSVPGMEWKAYRPGVNNLGDPNVSRVPADS
                     LIKFSNFLKSKFGYETGPFEDYGTFAVENYKILGRLDWNISQRHKLSIRFNTVRNTND
                     QLLNATSAPNPRASSSRVGVNAMSFKNSNYGFLNTVYSLSAELNSNLSSALSNQILGT
                     VTLIQDTRTSPSEIFPFIDIWDGSGDAYMSAGYELFTFNNNVVNNVYTIVDNVTYKTG
                     RHNITSGISFEYLYFGNSYMRYGTSYYRFKDLQSFVNHVNGVPGYQPTAFAITYSFDP
                     NNPKPVAELGFGQLAAYAQDEYNLLDNLKITYGIRFDVPLYLNELKSNPAVAALTFDN
                     NVKLDLGKWPETKVIPSPRVGFNWDVFKNRVLKVRGGTGIFTGRLPFVWFTNQPTNSG
                     MLQNTVELTSTATLDSIKFNPDPFYNMGATNYNTTTKTHDPLFPMTAGTKPPSSIAAI
                     DPQFKMPQVWRTSLAADIKLPLDMSLTLEGTYTQDINAIVQKNLNLKPADIAHYDGPD
                     RRPVWSTAGRKVYSNLSEVMLLTNTNRGYSWTFTAQLNFPIVRNFNGMFAYTASMAKD
                     ITGNPGSQAASAWSNNLSVRGQNDLDLSYSQYLTPHRLVGSLSYRFEYLKHAATTISV
                     FYSGYVAGNFSYRYSNDFNNDGVNADLIYIPKDQTEITFEDYIVGGVVKYTAAQQASA
                     FWAYVAQDKYLRNHLGQYAERNAAFFPWYHRFDVKILQDIFVNIGGKRNSLQVSADIL
                     NFGNLLNPNWGVRKELTLNNGSILSSRIVSNKPVFKMVEAGGKLPTSTFKNVNTTAST
                     WGLQIGLRYTFN"
     gene            16337..18826
                     /locus_tag="PLG42_00540"
     CDS             16337..18826
                     /locus_tag="PLG42_00540"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOU29725.1"
                     /translation="MEATIKYFKGDELAARVWTNKYALKDSYGNLYEKTPDDMHRRIA
                     REIHRIEKKYPNPLDEEFIFSLLKDFKYIVPQGGSMTGIGNDFQIASLSNCFVIGNEG
                     PADSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPKGMPVLNSALTSTGVVPFMERYSN
                     STREVAQDGRRGALMLSISIKHPDSTGFIDAKLESGKVTGANISVKITDEFMRAVEQK
                     SKFRQQYPIDSDKPKFIRDIDATELWNKIIHNAWKSAEPGVLFWDTILRESVPDCYAD
                     LGYRTVSTNPCGEIPLCPYDSCRLLAINLYSYVKDPFTKKAKFDFELFKEHVGLAQRI
                     MDDIIDLEIEKIDSILAKIDADPEPEELKLVEKNLWKNIRKKTEEVRRMGIGTTGEGD
                     MLAALGLRYGSDDAIDFSVEVHKTLALEAYRASVYLAKERGAFKIYDPNREKNNPFIK
                     RLKQADPALYESMVKHGRRNIALLTVAPTGTTSLMTQTTSGIEPVFSLFYKRRRKVNP
                     NDKDVKITFRDEVGDCWEEYPVLHHKFIEWLKVNNYDPDAVMQMSDQEINEIIARSPY
                     YRSTASDIDWVAKVKMQGAIQKWVDHSISVTINLPADVTEELVSELYMTAWKSGCKGV
                     TVYREGSRNGVLVNATNNQGTVVERPKRPKVLDCDVIRFKNNEEDWIAFVGLKDGRPY
                     EIFTGMSDEDTLLIPKNIVKGKIIKVKDEDGNTRYDFQYTDKYGYKNTIGGLSRMFKP
                     EFWNYAKLISGVLRYGMPIPDVVNLIQSLKLDSDSINNWKNGVERALKRYIPDGTRAK
                     GKCKDCGSETLVYQEGCLICNNCGSSKCGGS"
     gene            complement(18838..20031)
                     /gene="kbl"
                     /locus_tag="PLG42_00545"
     CDS             complement(18838..20031)
                     /gene="kbl"
                     /locus_tag="PLG42_00545"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOU29726.1"
                     /translation="MYQSFKKHLENQIKEIKEAGLYKNERIIVSPQGPEITLDTGQKV
                     LNFCANNYLGMSNSSELVEAAKEAMKTHGYGMSSVRFICGTTDLHKKLEKKIAEFFGT
                     EDTILYAACFDANGGVFEPLLTEEDAIISDALNHASIIDGVRLCKAQRYRYENANMED
                     LEAQLKLAQAQRFRIIVTDGVFSMDGNVAPLDRIVELAEKYDALVMVDESHSAGVVGR
                     TGRGVTELFNLMGKVEIITGTLGKAFGGAIGGFTTGRKEIIEMLRQRSRPYLFSNSIP
                     PLVAAAGIKMFEMMSATNELQDRLHANAEYFISKMKEAGFTIKPTQSAICAVMLYDAK
                     LSQDFAARLLEKGIYVIGFYYPVVPKGQARIRVQLSAAHTKEQLDKAIDAFISTGKEF
                     GLIKS"
     gene            complement(20258..21292)
                     /gene="tdh"
                     /locus_tag="PLG42_00550"
     CDS             complement(20258..21292)
                     /gene="tdh"
                     /locus_tag="PLG42_00550"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOU29727.1"
                     /translation="MKTMKALVKSKPERGIWMEEVPVPEPGLNDVIIKVKKTAICGTD
                     LHIYRWDDWAQRTIKTPMIIGHEYMGYIEKMGAGVINLKEGDRVTGEGHIACGHCRNC
                     RRGKEHVCENTVGVGVNIDGAFAEYVKIPASNIVPLDKRIPDDWAAIMDPFGNATHTA
                     LSFPLLGEDVLVTGAGLIGSMAIAIARYAGARFIVASDLSDYRLEIAKKMGATLTVNP
                     SKGEKISDAVKKLGMRGFDVGLEMSGSPAAFREMIQNMYNGSSIALLGILPQVCEIPW
                     SDIIFKAITIKGIYGREMWETWYKMEMMIIGGIDLNPVITHRFHVDDFQKAFEVMEEG
                     NCGKVIMDWN"
     gene            21437..22945
                     /gene="gltX"
                     /locus_tag="PLG42_00555"
     CDS             21437..22945
                     /gene="gltX"
                     /locus_tag="PLG42_00555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOU29728.1"
                     /translation="MVRVRFAPSPTGPLHIGGVRTALYNYLFARKHNGTFILRIEDTD
                     QTRYVEGAEEYIFESLDWCGIKVDEGVREGGPYGPYRQSDRKNIYRQYAEFLINKGHA
                     YYAFDTPEELEMLRKKYESQKKTFLYNAEVRNELKNSLSLPEQQWKEMINSGIPYVIR
                     YRMPVNEEISFDDMIRGHITVNSSTLDDKVLFKSDGMPTYHLANIVDDHLMKITHVIR
                     GEEWLPSLPLHVMLYRSFEWEAPLFAHLPLILKPDGKGKLSKRDGDKMGFPVFPLYWP
                     YGETARGYREDGYYPEAFVNMLALLGWNPGTEQEIFSMEELIKEFSLERIHKAGSRFD
                     PVKARWFNHVWLQKRSNNQLAHEFSEIIKGKGYSCDIARLETLVGLIKERVDFVKDMW
                     DEAYFFFSPPESYDSDALRKKWNEESPLLLAEVSNLLRKIEDFTPVNLEKNVREWIEK
                     KGCNSGTIMHALRIAVVGKLKGPGIFDIISWLGKDETLNRIEKALATIVQQS"
     gene            22942..23619
                     /locus_tag="PLG42_00560"
     CDS             22942..23619
                     /locus_tag="PLG42_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU29729.1"
                     /translation="MNLPLADDFIDIHNHGASSATGIFSVENITADEERVPDDSHGLA
                     YTIGIHPWHVTVKNYNEQLKKVILYSRHSNVIALGEAGFDRLRGPEINFQKEIFEKQV
                     IISEEVKKPLYIHCVKAWEELLSAHKMLKPHMPWLVHGFRGKHELAMQLITRGMYISL
                     WFDFVMRPESTQLLKSLPFNRIFLETDGGDTKISTIYEKVATDLDLTVDQLKSQLYSN
                     FRTFFGV"
     gene            23619..24359
                     /locus_tag="PLG42_00565"
     CDS             23619..24359
                     /locus_tag="PLG42_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOU29730.1"
                     /translation="MEEWLSRTELCLGKERLEKIKSSNILVVGLGGVGAIAAEMLCRS
                     GIGRMTIADGDIILPSNRNRQIHALISTERQPKALVMEKRLRDINPELQLTVINEFIR
                     DERMIEILEEPFDYVVDAIDTLSPKVFLIYHSLRRNYRIISSMGSGGKFDPLLIKVSD
                     ISETSFCHLARMIRKKLHKLGIREGFKAVWSPEIVEKSRIIRTEGERNKASVAGTVPW
                     MPAAFGIVIASVVIRDLAGIEINASDIQ"
     gene            complement(24368..25357)
                     /locus_tag="PLG42_00570"
     CDS             complement(24368..25357)
                     /locus_tag="PLG42_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU29731.1"
                     /translation="MNTGYRDRIFNIKHPEEFEDIAIEVFHYQVQHNPVYRQFTERLG
                     IEINGVQRIEEIPFLPVEFFRNHTVITGSREAVMVFESTGTTGTIKSRHYITDPSLYD
                     ESLLRGFKFFYGDPRDYLFAALLPSYLERPNSSLIYMVNKLIDSGASERSRFFERGSW
                     KLIDTLKLAAGEGKKVMLIGVSFALLDLAEKFSSDLSGIIIVETGGMKGRRKEITREE
                     LHNILIESFNVPAIHSEYGMTELLSQAWSQGKGKFSSPPWMKILVRELNDPLSVSAAP
                     GTAGAINVIDLANINSCSFIATGDIGRLQSDGGFEVLGRYDHSETRGCNLMAE"
     gene            complement(25354..25899)
                     /locus_tag="PLG42_00575"
     CDS             complement(25354..25899)
                     /locus_tag="PLG42_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29732.1"
                     /translation="MRPLFLFLVAVAVLLTGCRKEDKPVYSGIMTINNKRAGVSPTYY
                     IEGFHVPTGKKVPDLNNKLDIISVLSDYDVNFNPLKIIFITNNFKNSFYRYGQYSDTK
                     SAEDAFNALTEFTEPVWTEIGDSVRINQIWIYRTSDSRYAKLRIISTILEKRPDMQDP
                     YAECTFEWVFQPDGTKTFPPR"
BASE COUNT         7735 a         5781 c         4971 g         7538 t
ORIGIN      
        1 caatcctgtt tgtaagaacg tcaagatgat tcatagtcag attttctgtc tgacctagcc
       61 ggataatctc ctttattact ctgtcatgct gtgcataacc ttcagaaaat aaacacaaca
      121 gacaaaatac aatcacatat agtttgaaaa gtttcatgtg cagaatattt atttatattt
      181 gattatcaaa ttttcccatc ttctttaaaa tagctttaaa ctctatcaga atgaggtacc
      241 ttttaattgg aataattatt tctttagtag ctgatttcaa tttcatatcc acttatggtt
      301 gcaaaatcat atttttttca tcactacaat aatcaaaaaa cttattcatc aaaatcaaag
      361 acccggcaaa aataacattt atatcttact aaactcattt gcaggtaact ggtttcattt
      421 tatttaacag tttttatttc cggtggtatc aacttgtaca ttacaggtgt taccagtctt
      481 gcaaaaaggg ttgaggttat taaaccccct ataatcacat atgcaagagg agaataaaga
      541 gctgaaccac tgagtgcaat tggcataagg cctccaacag ctgttagtgt ggttagaact
      601 acaggaagga atcttatctc acctgccttt tgaattgctt catcaagaga gaccccttct
      661 tctctgagct ggtttgtaaa atccacaagc agtattgagt ttttgatttc tattccgatt
      721 aaagccacaa aaccaacagc agcagcaaac gagagagtgt tacccgtgat ataaagcatc
      781 agtatacctc caataattcc tagaggtatt actgatagta ctatcagagt gcccttaaat
      841 gttttgaatt caagaagaag aatagtaaat atcccgaaaa cagcaataag tattgccgac
      901 catattccgg caaaactttc acgcctgctt tccagctcac ctgcagggat aatcctgtag
      961 ccgtcaggaa gtttaagctg gtaaagtttt tcaagtacct gtctggtaag ccggtcggta
     1021 ttgtagcctg tcataactga tgccgaaact gtaattgacc tttccctgtt ataatgctgg
     1081 atcacagtgg gtgaatcaga gaattccacc ctggcaatct gtgaaagagg tatcagagag
     1141 ccggtcagag aagcaatata gatactgttc agtctgtcaa tatcagcctc tccacgatga
     1201 acctgcctta ccatcagggg gtactccttt ccgtctgagt ctctgaacct gccagcttca
     1261 atacccgcaa gggcaagcct gatggtcctg tccacttcta ttgacggaat tcccagcatt
     1321 cctgccttct ccgaatttac ctcaatcctg aggtcggtaa gcgactgact gagaggattc
     1381 ttaatataaa ttgtaccggg tgtgcttctg aagatttctt caattacctc cgcatatttg
     1441 tagagtgtat ccaggttgtt acctacaagc ctgaccgcta ttgcagcatc aaccggcatt
     1501 ccgttttcaa attcattaac atatatctta ccatttggat aagtattcag agtatctctg
     1561 agccattcaa caaacatttg caattgtttc aggtattccg gtttaacctc gcacattact
     1621 tcacccaggg ttgtctgtcc gctcttctga aaagaattgt aataaatcat cggattacct
     1681 ttcccgatat ttgacataaa atactttact tcgggccttc ctgaaaggac cgattcaaca
     1741 aattttactg cacggtcggt ctcatttaaa ttgctcccgc gtggtgtttc aacagtaatc
     1801 aggaactgtg gtatacctgc tttcgggaaa aggctgaaac cgataacctt aatcagaaaa
     1861 agggaagctg taaaaagtat agtagcacct gttaaagtct tgaccggatt attgagcgac
     1921 cagtgcaatg cctttgaata tgtgaagttt attgctttat gaaaggccct gagaactacg
     1981 tttcctctcg gatcggtatt acctttgagc atccggctag caataaatgg tattatggtc
     2041 agagccacaa acaacgaacc tgtaacgacg aatgcaattg ttgtgggaag cgatctcata
     2101 tacttaccag ccatgcccgg cagaaacatc agcggcagga aagcaaaaat aagtgttgcc
     2161 gtacagccaa gaaccgcagt acctatctgc cttgttgcac ctattgccgc ttcagaagga
     2221 cttttccctt cccttaccca tctcgagata ttttcaatga ccacaattga atcatcaaca
     2281 agaagtccaa gagctataac tccgcccaca atacttaact gattaatgct gaatcctgta
     2341 aagtagagtc cggttacacc aatcataagc gaaaggggaa ttgaaacaag aacggtgccc
     2401 gaggctctga aacccagcgg tagtaaagta acaatcacga ggaaaaatgc gaaaagaaaa
     2461 tctttttcaa gacgcgtcag ttttctcctt acattctgcg actggtcaaa acctctttca
     2521 agcctgatcc ccttcggcaa cttcttttcg aaagaatcat aaaccttata tatatcattc
     2581 cttaccctgt ggatgttata accttttttc atactcgcaa ttacaaaagc tgccttttct
     2641 ccgttgtatc ttccaaaata tttcttatcc tcattgctcc acattacctg tgctacatct
     2701 ttcaggtaaa caatttgccc gttatcagag cctacgattg tgtttcttac attttcaacc
     2761 gactgatatc caccggttgt tctgatgtta aatttctttg gtcctacatc tatacttcct
     2821 gccggaatgt tggcattttc actctgtatg gcagctataa tcctgcttac tgtcagcttt
     2881 aactgtgcca ttttactgag gtctatttca atccttacct ctttcgcagg ataagcaagt
     2941 gcttcagctt ttcgcactcc cggtacagac gagacaagat cacgaagttc ttcggcataa
     3001 tcgcccagtg tttcgtaaga tgctgtttct gaaacaattg cactttgaac aatatttgta
     3061 tttcctgcct gaattttaat tgcttccaga ctgtaaagtc cttcaggaag tgaaggccga
     3121 agagaattaa gttgtcttaa aatctcatca tattttttgt caggatcact ttccgctgta
     3181 aattcaacca caatcactgc cactccatcc tttatttccg tttttattct tttgagatct
     3241 tcaagctcat taatgcttct ttccaccttg tcgaccacaa gttgttcaat atcaacaggg
     3301 gaagctcccg ggtaaacggc tataaccgga aatattggta tcggaaactc gggatcctcg
     3361 gcctggggta tttttatgaa agaaattatc cccatcacaa caagcatcaa aaacactatt
     3421 atcgtaaact gataatttct gacagccagt tccgaaattt tcataacagc tttaatttaa
     3481 gacatttact ttcataccgt ccttcagata agctgcgcct tcagaaacaa caagggtaac
     3541 gtctcctgga agattctcca ctgctatttc tgacccccgg atgcccgtta taaccacttt
     3601 tatctttttc actgttgatg aatcagttag ggcaaagatg taccctgtat taccgtcagc
     3661 atcaacaact gagcctgccg gaacataaac ggtatttttg cttaccgacg gaaatatatc
     3721 aacacctgca ataaatccgg ctgctaatct gtagccgcca tcgctgaaac ggatttccac
     3781 atcgaaagta cctgtaagag gccctgcaag ttcacctgtt ttctcaacaa tacccggcaa
     3841 gcgtactcca ggccatgcat cgggcattac cacagcagaa tcaccctgtc tcaccctgat
     3901 tatatccctg tcggcaagcc cggctgtcac cttccacatt ttgcctgaaa ggccgaaaag
     3961 aaaaacagga taacccgccg caaccagctc gttttcacgt acaagctgtt taagtattat
     4021 tccatcggcc ggtgcgacaa tttttgaccg ttcgagatta aagtttgcta tatcgaggtt
     4081 cgactttgcc atattaagcg ccgtagttgc gttctgaagc tgttcaagcg ttgccacact
     4141 atcggcataa agatttctga cccttgtgta atcccgtaat gccttttcat aggcattgcg
     4201 ggcaagttct gcctgcgaac tgatttcaga aagattaagt gaggcaagca gtgtcccttt
     4261 cttaacacgg tcaccttctt taactaaaat atgctcaact ataccccctg tcttgaatga
     4321 gagctttatc tcttcttctg gcatgattac acctgctgca tgcacaggca gactctcctg
     4381 tgaagatgca actgaaacca ccttaacatt attcaaatct gcaacaatgt gttgttcctg
     4441 tctgctttta caggataaca ccagaaattg cagaattaat aaaaacagaa tttctttttt
     4501 cattttatag aaaatttttc gtatatatct gaatttatta ctgttactct ttcaaaatcg
     4561 gcgaaacaag agaggtaatt aaactttgat attacaagat tctcttcagc ctgtgtcaga
     4621 tttgagcgtg catcaagaaa ttcaagcagg cttgcctgcc cttcttcata ttttctctgc
     4681 actagccgga acccttctga agcatttctg agccttttct ccgcagttag tatctctttt
     4741 tctgaagtaa agagttcact caaagttgta agtacttcca gctcaatctt tttctttgcc
     4801 tcttcgagct tcgaatcggc ttcggcttta tcaagcatgg cctgccttat ttttgcacgg
     4861 ttacggtaac cttcaaataa ggtccatgta agaacagctg aggcctgaag ataatcctgc
     4921 tctttgttga atctatattc ttcaccctga aatccgtaat caaacacaaa atagatgtcg
     4981 ggaagtttat ttgcctggtt catcttaacc tgcaactctg ccatattctg atatttcctg
     5041 agcttttcga gttcctccct gccagaaatg gcagaataag tgtattgttc agtaacagga
     5101 ctgatagtag ggaaactgtc gggaaaagag gtgattaccg gtgtctccag tggccggttc
     5161 aaaagaaaat tgaaataggc aaccgcagtt ttcctattgt tttcagccct ttgaagctcc
     5221 tgttcatacc ttgcaagttc agattcggag cggtaaacaa aatcttttgt aaccttgtca
     5281 ttttcaaaga gtttcctgct tacccgtaca ttttcctcaa gaagctttcc tgtctcttta
     5341 agaatatcaa gcatcgcact ggccattgca acattgtaat aggctttctt tatttctgct
     5401 atgagttctc ttctgtattg attcaaatca acttcattgt aaagatttat ctctttcctt
     5461 atctgtgtgt tataataaat ttccggactg aagacaggtt gagtaaccct tattttagtt
     5521 tcatgttctc tcggacgcag aaactttata acctgatcat caattggaga gaagagattt
     5581 gaatgggtaa gagcattaag tgtcatgaaa actggattca tgagagtgcc ggcaggaaat
     5641 tcaatagtcc tgccaccttc tgaacgtgtg tatcttgcaa taaagcttat tcccgggtag
     5701 aaaagagccc ttgcttccct gagagcttcc agacttttct ggcagtcgag agtcttctgt
     5761 ttcagcacca ggttattttc aagtccatac tgaatatact cttctagaga ttcctgaccg
     5821 cagagaaaag gtgaaaataa aactgctgca aaaagaatat attttttcat ttttctgaat
     5881 ttttatcttt tatctatcct ctctttcata tttttcagaa tagcatcaaa aagtcttccg
     5941 accagttccg catcctcagg cttcgcctga cggtatagtg aaccggcaaa cgcaaatagt
     6001 tcctttcgaa atgactcctg agccctgtaa accgttagcc ccttttctgt aagattcaac
     6061 aaaacctctt ttttgttttt tgtctgcttt cgcagaatga gcccttttgc agaaagtttc
     6121 ctgatagtct gtgaaatagc accctttgtc acacccataa gttctgcaag acgtgtaata
     6181 ttcaatccct gattcctccc aatcattgca agagtatgaa cctcagtaaa atataacatg
     6241 tcacccgttc cgtaatcttt tggttttctg gttattagag aatacagatg aagtattctg
     6301 agaaactggt caataagttt ctgaagctct ttttccattt cttatattgt ttagatgcta
     6361 aacaaaatta taaaaagttt agatactaaa caaattttaa gtaaattcat tgaaaaactc
     6421 tggagctctt atttggggcc cctttgtcta aggaagaata tatttgaaaa tcttcaggat
     6481 tagcagggaa ccgctgccgg attatctaag gtttccgtac ggttgttatt ttctggattg
     6541 tttgtatttg ctgatatatt ttttacgaaa ctccttaagt aaagcttcct cttcctctcc
     6601 tttctttact ccttcaataa gaattttgca aaggccctct ataacatcat ccgggaaacc
     6661 tgccgctatg gcataacgtc ttatcatctt tttctcagct tccgttatca cattgtctga
     6721 gagaataagc tcggtgatat tgtaaagttc aaagaattta tcctcaagac tgtatggcgg
     6781 atgataattt aaaggatgct cattatttat aatgtcatct atttccgggt cggtaagccc
     6841 gaatttcctt ccttctttat gaagaatttc catctcagtt tcgcttattt tcccgtcaag
     6901 tttcgcaacc tgaaccagat gaatgaaatg atccctgttg attcgtttac ctgtgctggt
     6961 aataaaattt gagaaattca tattagcata catttgagaa gcaaaattat ataaaagtct
     7021 gtcacactta tctcatcaca gtaaaaaaca tttaaaattc tgttttgttc ttaagaaaat
     7081 aaggtattgg aataaaatag gaaaatcatt tgtcattaat tataaaagac tactattatg
     7141 aaaaatccat ttctttgcag gatgtggttt attccactgc tttttacatt atttcttact
     7201 cctgtggcaa atagtcagag tttaaaagaa aatccagcgc tcgatgagca ggtgaggaaa
     7261 ttcctcaatg aaaactcacg aaggtggtac gacatgaatg tacctgaggt tgacgggaag
     7321 cttttatatg atataattat aaaaaacaac tataaaagtg ctcttgaaat aggcacttca
     7381 acgggtcatt ccggcatctg gattgcatgg gcattaagta aaacaggtgg caaactcatc
     7441 acaattgaaa ttgatgaggg ccgtcacagg caggctgttg agaatttcaa aaaagcaggg
     7501 ctatcagagt atattgacgc caggcttgct gatgcgcatc agcttgtaaa agagttaaaa
     7561 ggtccttttg atttcatttt cagtgatgct gacaaggaat ggtataaaaa ttatttcata
     7621 gagctcgacc caaaattaaa ggttggagga tgtttcacgg ctcacaatgt atatgattat
     7681 ggatctggag gcaggggttc aaggggtggt tttagaagcg ggcaacagga atttcttgag
     7741 tatgtccaca gtctcccgaa ttatgaaaca accgtcgaca accgtggtgg tggagtatcg
     7801 ataagttata agaaaagttc cagataaccg gcatcaaatc cccagctttg cctttatggc
     7861 aggaggcaca ccatttttat gcaccataat aaaaatggtt tttgcccgta cataattttc
     7921 tgacatgttc agatagccac caaacggatt tctttcggta ccccatgaat ttttcgtctt
     7981 gtaatacttt gtgccgtact ggtctttcag caaccccgta atgtgcatca tatggtcgtc
     8041 ggtggtagaa aaattttcat aacctgcctg cctaatttcc tgtgttacct taatttcagg
     8101 aacaggacgg tttcccgcag tcttggtata aataacctga gatccctcat tttctgctct
     8161 tcccatggac gtggaataat tgtcgccccc ttctgttgca ggcaaaatcg ctatccctct
     8221 tgagtgcatg aaaccacttt cgctcacatc tccatcccat gcaatggtat acccattggt
     8281 gagtgaataa tccattatct gtataagctc atcaagaggc acattgtaaa atttttccat
     8341 acgccagttg tcgggtacct caacaacccc cctcgtataa aaagggaaat gagtaaatga
     8401 tgttatctca acataatctt caggatttat tcccagactt gctgcaaatg atttcggtgt
     8461 ataactcttc ccctggtatg taaatgtttc aggaactttt ccgaagtaga tatcaaaaat
     8521 tgcatccaca atctgatggt actgcgggct ttcatttttc cttcgaaccg gaacttctgc
     8581 cacagcattt atgaaagcat tcagttcgct gtgattatga attgtcgatc catagttgag
     8641 gccagaataa acttcttcag gtactatgcc ttccctcgaa aacactatca tccagtcgtg
     8701 tgcaaggctg ccttcaccaa ggttacccct gccctgcctc aggtaattgt ctttcagctt
     8761 atcaatataa ttatacctta caacaaacat ttcagaaagg tcatactctc cccttcccat
     8821 cctcaggagt tcagactcta tgaatgaagt ggttgcaaaa caccagcatg tgccggtgat
     8881 tgcctgattc tttactgatg tggctttaag gtctgccacc gtagtaaaaa catatccttc
     8941 atcagttgaa gtctgcttct gtgatgcagt ttttatttgc tgggcaaaaa ccggaaaaac
     9001 aatgctcaga aggaaaagtg aagcaaataa tttagttttc atataattca atttatctca
     9061 atgtggaata aaatgcatct tctatgtgtt caacagtaaa tgattttccg tcggaaataa
     9121 ccgttatgac atcgaaccgg ctctccttgt caatattgta aatccttatg taactgtcgg
     9181 cagcatatat gatcattttc tgttttttat cagtcacggc atcacgtgga tggatataat
     9241 aatcgtcctt tcgtgtttta acctccacaa aaaccacata attttcgttc tctgcaacaa
     9301 tatcaatttc cttctggccc gatttccagt tccggtgtct gattttatag cctttttcca
     9361 ccagatggcg gactgctata tcttcacctg ccgggcctat gttattaatg tcggccattt
     9421 tattcagatt ttaatcagct tgtttcttat tgcatacagt ataagacttg ctgtattttt
     9481 cgaattggtc ttttcgagaa gatttgcacg atgcttgtcg acggtgcgct tgctgataaa
     9541 aagtgcattt gctatttcct ggtttgaaag tccttcacat attttctcaa gtatctcttt
     9601 ctcacgcttc gacaggacgg caacttttgt ttcagcttcc ctgtgtttga ttttctgaat
     9661 gacattctga agcagctgct gggagaaaaa tgtcccgcca cgctttacag tatttattgc
     9721 ttccctgact tcggaaatgt ctgaatcttt aagaatgaaa cctcgcgctc cggcatcggt
     9781 cattctgtaa taataatctt cttcaccata cattgaaagt gcaatgacct ttatttcagg
     9841 ctttctttcc agaaccttga ttgtagcttc aaccccatcc attaccggca tattaatgtc
     9901 catcaataca atatctgccc gggttctgtc gataatatca agaagttctg ctccgtttga
     9961 agcttctccg gtaatttcaa attccggaat accttcaagg agaagcttca gcccgttacg
    10021 gaaaagatga tgatcgtcaa caataattat ttttattttt tccatcttct gctttatcag
    10081 actataatct ttatcaatgc atttgttcct ttgcccttgc tgctttcaat aaagaaaact
    10141 ccgtcgatgg atttcacacg tgtatcaata tttgataaac ccatcccctt gcaatcttca
    10201 gtaccaagta tcgaagtgtc aaatcccctg ccattgtctg aaaactgaag tgtgataaat
    10261 ttatcatgtt tgttaagttc tatttcaacc cttgaagccc ctgaatgtat cagtgaattg
    10321 ttaattagtt cacaaaccgc tctgtatatt acaacttcct tgtcattgtc aagtcttacc
    10381 ccttccatgt tcgatttgaa atcgatctca agggcccggg ttttgttaac ttttgaagtg
    10441 aaggcgttta tagcacttga aagcccaaaa tttgtaagaa catgcgggct gagattgttt
    10501 gagatctcct tgatagtatt aattgcctca ttaaccacat ggttcgtatt ctgaagtata
    10561 tcaattccag cagggtcggt gattttccca ttcagagccg aaagtgacat cttgactgtg
    10621 gaaaggaggg gacccagacc atcatgaagt tccttcgcaa atctcttctt ttcgttttct
    10681 tcagtgttaa taattgcatt aagaaccctt cgctcagttt ttagcctgtc ggtttcagcc
    10741 cttttaaggg aatagaatat ctcacgtatc agtataaccc ctacaataat cataaatgat
    10801 atgaacaccc ccatccattc atctatcatc tgccatgtaa aagagggtgt accccgcagt
    10861 agttcccaga gctgtataat ttttcttatt gccatgaaaa gaaatgagaa tgaaagcaat
    10921 atccacgaaa gcctgtactt tgtcatcttg acaaaactca gagctataat tgcagcaata
    10981 acctgaagcg caatagaaat aataagggca atcagccgta ccattcaaat ggcaattatt
    11041 ctgcaaaata acaagtttat tctgttccaa gcaataaata tgtaacagat atttaaccgc
    11101 ataattttta caggaacctg aggtttttta tctttgccgc gaaatttttt cataatgaac
    11161 tttctttact ctctggctgt aagattatat gtgtggagca tttatgccgc atctcctttt
    11221 aatttaaaag ccagattatg ggtgaaaggc aggaaagggt ggaaagaaaa actgtcatca
    11281 aagtcagaat atttaaacaa tcctgtatgg atacactgtg cgtcactggg agaatttgaa
    11341 cagggaaggc cgcttattga aatgataaaa gaaaaaaaac ctgatcagaa aattcttctc
    11401 accttttttt caccatcagg gtatgaaatc agaaagaact attcttttgc cgattatata
    11461 tgctatctcc cctccgacac acgaaaaaat gcaaagtatt ttattgaaac agtaaaaccg
    11521 gtaatggcaa ttttcataaa gtatgagttc tggaataatt atatatcgga actctactta
    11581 aaaggaatcc cgttgtatct ggtctccgga attttcagaa actcacagca tttttttaaa
    11641 tggtacggaa gtttttttct tgggatgcta aaaaaattca cccatttttt cctgcaggat
    11701 gaagcttcgg cagagcttct tagtaaagct ggaataaaca attttacagt gactggtgac
    11761 acgaggttcg acagggtatc aaaaatcgcc gcatctgcac cggtaatacc tgtaattgaa
    11821 gagttcagaa acggtgaaaa agtttttctt gcaggaagca gctggcctga agatgaaaaa
    11881 attattgtta agtacatcaa cagcaatccc ggaaggatga aatggatatt cgctccacac
    11941 gagccggaac cttcaaatat tgacaggctt gaaaggatga taaaaacaaa ttgtgtcaga
    12001 ttttcagaat tttcgggaaa gaacagggat gcaagggtat taataattga taaaatcgga
    12061 attctttcat ctgcttaccg gtatgcatat attgcagctg ttggaggggg ctttggaaag
    12121 ggaatacaca atattctgga gcctgcctgc tggggtattc cggttatgtt cgggccaaac
    12181 taccggaagt tcagagaggc tctcagcctg cttggcagaa aaggtgcatt ctgtttcaga
    12241 aattttgaag aattcagcac tatcctcgat gaacttctct ccagcatgga actctataaa
    12301 tctgcttcag aagccaccct gaaatatgtt tatgagaata caggggcaac agataggatt
    12361 ttttcgtcaa tttttcaaca agatattaac aacaatcagg tttaaatctt gtttataatt
    12421 tcatattatg ctttataaat gaaagacttc aagaaatata atttggcact ctggtatttt
    12481 ccagcaaagg tttgtacgga aaaaatgatg ggtattgaat tttaatattt tacataaata
    12541 aatcctgaaa ctatgatcaa aaaaacaaga gtaatttttg gagttctgat gtttttggcc
    12601 ttttccgcgg tttcgttcgg gcagggtgca acaacatctt caatggcagg taaagtaact
    12661 gacaacagag gtcaggccct gccaggtgct acagtagttg ctgttcacgt tccatccgga
    12721 actgtttatg gtgcttcggc aaatgccgaa ggtttctttc tgatacaggg tatgcgtccg
    12781 ggtggccctt acaaagttga agtatcattt gtgggctata ccaaaaagac ttataccgat
    12841 ataactcttt tccttggtga gactttcgac cttaatgcct cccttacaga gagcagccag
    12901 gaacttaccg aagtggttgt tgtaggagac aagccctcag catttaactc actcaagacg
    12961 ggagtttcga tgaatatcag aagccaggac attagctcac ttccctctat cagcaggagc
    13021 ataaatgact tcatacgcct ctcaccgctt tatggaggca gcaacagttt cgcaggccgc
    13081 gacggacgtt acaacaacat agtcattgac ggttcaaact tcaacaacaa cttcggcctc
    13141 agcagcagaa acatgcccgg cggtgatgca gaacctgttt cactcgatgc tatcgaagaa
    13201 attcaggtta atgttgctcc tttcgatgta agacaggcta actttaccgg agccggtatt
    13261 aacgcaatta cccgcaaggg aaccaacgaa tattcggcat cagtttatac ctattaccgt
    13321 gatcagtcat tcaacggaac aaaagtgggc gatttcgagc tgcctcccgc agctaaaaca
    13381 tcaacaaaaa ttatcggtgc aagagtgggc ggtccaataa taaaggacaa actcttcttt
    13441 ttcattaatg gagaatatga aagctcttct gtcccgggaa tggaatggaa agcctacaga
    13501 ccaggcgtaa ataaccttgg cgatcccaac gtaagccggg ttcctgccga ctcactcatc
    13561 aaattctcaa atttcctgaa atcgaagttc ggatacgaaa ccggaccgtt tgaagattat
    13621 ggaacattcg cagttgaaaa ctataaaatt ctcggccgac tcgactggaa cataagccag
    13681 agacataaat taagcatccg cttcaatacg gtacgcaata caaacgacca gctgcttaat
    13741 gcaacaagcg ctcccaatcc aagggcatct tcaagcagag ttggggtaaa tgcgatgtcg
    13801 tttaagaatt caaactacgg attcctgaat actgtatatt cactctcagc tgaacttaac
    13861 agtaatctct catctgcact ttctaatcag attctcggca ctgtaacttt gattcaggat
    13921 acgagaacaa gtcctagtga gattttccca ttcattgaca tctgggacgg ttcaggggat
    13981 gcatatatga gtgccggtta tgaactgttt acttttaaca ataatgttgt caacaatgta
    14041 tatacaatag ttgacaatgt cacttacaaa accggccgcc ataatataac aagcggtata
    14101 tcatttgaat acctttattt cggcaattca tatatgcgct atggtacaag ttactacaga
    14161 ttcaaggatc tgcagtcatt tgtgaaccat gttaacggag tacccggtta tcaaccgact
    14221 gcttttgcaa taacctattc attcgacccc aataatccca aaccggttgc agaacttggt
    14281 ttcggacagc ttgctgctta tgcacaggat gaatacaatt tgcttgataa cttaaaaatt
    14341 acctatggta tcaggtttga tgtgccgctc taccttaatg aattaaaatc aaatcctgct
    14401 gttgctgctc ttacatttga taataatgtg aagctcgacc tcgggaaatg gcctgaaaca
    14461 aaggtaatac cttccccgag ggttggtttc aactgggatg tcttcaagaa cagagtactt
    14521 aaggtacgtg gtggaacagg tatcttcaca gggcgtctgc cttttgtctg gttcaccaat
    14581 cagcctacaa acagtggtat gcttcagaac acagttgagt taacatcaac tgccacactt
    14641 gattccataa aattcaatcc cgatccgttc tacaacatgg gagccacaaa ttataatacc
    14701 acaacaaaaa ctcacgatcc tctgttcccg atgactgcag gcacgaaacc accaagctcc
    14761 atagcagcaa ttgatcctca gttcaaaatg ccccaggtgt ggaggacaag cctggcagcc
    14821 gacataaagc tgcccctcga catgagcctt actctggagg gaacatatac acaggatatc
    14881 aatgcaattg ttcagaagaa cctcaacctc aagccggccg acatagccca ctatgatgga
    14941 cccgacagaa ggcccgtatg gtcaacagca ggcagaaagg tctattcaaa cctctcggaa
    15001 gtcatgctcc ttaccaacac caaccgtggt tacagctgga ctttcactgc acagctcaat
    15061 ttccccatag tgaggaactt caacggtatg ttcgcctata ctgcttcgat ggcaaaagac
    15121 atcaccggca atccaggctc ccaggcagct tcggcatggt cgaacaacct gtcggtcaga
    15181 ggacagaacg acctcgacct gagctattca cagtacctca ccccccaccg acttgtgggc
    15241 tcactcagct acaggtttga atacctgaaa catgcagcta caactatttc agtattctac
    15301 tcaggttatg tggcaggcaa cttctcatac aggtactcca acgacttcaa caacgacggt
    15361 gtaaatgccg accttatcta tatcccgaaa gaccagaccg aaattacttt cgaggattat
    15421 attgttggtg gagtggttaa gtacaccgct gcacagcagg catctgcttt ctgggcatac
    15481 gttgcacagg acaaatacct caggaaccac ctgggacagt acgctgaacg taacgcggca
    15541 ttcttcccat ggtaccatag attcgacgtc aaaatactgc aggatatctt cgtaaatatc
    15601 ggcggtaaga gaaattcact acaggtctca gctgacattc ttaattttgg caacctgctc
    15661 aatccgaact ggggtgttag aaaagaactc accctgaaca acggttcaat actttcatca
    15721 agaatagtgt cgaacaagcc tgtattcaaa atggttgaag caggaggcaa actgccgaca
    15781 tccactttca agaatgtgaa tacaacagcc agtacctggg gacttcagat tggactcaga
    15841 tacacattca actaaattct taaaaattaa ttaatgtttg aaggggggtt atttaacctc
    15901 ccttcttttt taaagaaccc tctatcttaa tgtgctgttt attaattact tacatttata
    15961 actcgaacat aaaacacaca ccttcaggaa gaaaaatata aactcattaa cggttaataa
    16021 ctattgaatc aaatgttaat aaccttaatt tttttgactt gcttttagaa cctctgttat
    16081 ctaatttagc aactacccgc cttttttacc ggtggttgtc taacataatc tttttcagta
    16141 aataatagca taatacgttc aggatatggg agaacttttt gtcgagaaac cagaagtgtc
    16201 agaaaagaaa gattcaaaaa agatgacaca gccttcggca gttgaaactg atggtaaaaa
    16261 tattgcaggt gactgtgccg gagcaggtga aggcaaattg aataacccga aggttatatt
    16321 ttctcaggac gaagtgatgg aagcaacaat aaaatacttt aaaggagacg agcttgcggc
    16381 aagagtctgg actaacaagt acgcacttaa ggactcgtac ggaaatcttt atgaaaagac
    16441 tccggatgat atgcaccgca ggattgcaag agagattcac aggattgaaa agaagtatcc
    16501 caatccactc gatgaagaat ttatattcag cttactgaaa gattttaaat atattgtccc
    16561 tcagggtggt tcaatgacgg gaataggtaa cgattttcag atagcatcac tttcaaactg
    16621 ctttgtaata gggaatgaag ggcctgccga ctcatatggc ggcataatga aaatagacca
    16681 ggaacaggtt cagctgatga aaaggagggg tggagtgggg cacgacctct cccacatccg
    16741 cccgaaaggg atgcctgttc ttaacagcgc ccttacttct acaggtgtgg tgcctttcat
    16801 ggaaagatac tccaattcta ccagagaggt ggcacaggac gggagaagag gagctctcat
    16861 gctctccatc tcaataaaac atcccgactc tacaggcttt attgatgcca aacttgaaag
    16921 cggcaaggtg acaggagcta atatatcggt aaaaattacc gatgaattta tgcgcgctgt
    16981 ggaacaaaaa tcaaaattca ggcaacaata ccccatcgac tccgacaaac ctaaatttat
    17041 cagggatatc gatgctacgg agctgtggaa caaaataatt cacaatgcat ggaaatcggc
    17101 agaacctggt gtgctgttct gggatacaat attacgggaa agcgtacccg attgctatgc
    17161 cgacctgggc tacaggacag tttcaacaaa tccctgcggt gaaataccac tatgccccta
    17221 cgacagctgc cgcttgctag ccataaacct ctacagctat gtgaaagatc ctttcaccaa
    17281 aaaggctaaa ttcgattttg aactcttcaa agaacatgtc ggactcgccc agcgtataat
    17341 ggacgatatt atcgaccttg aaatagaaaa aatcgattca atacttgcaa aaattgatgc
    17401 tgatcccgaa cccgaagaat taaaactcgt tgagaaaaat ctctggaaaa acatccggaa
    17461 gaaaacagaa gaggtcagaa ggatgggtat cggcacaaca ggtgaaggcg atatgcttgc
    17521 agctctcggg ttaaggtacg gaagtgacga cgccattgac ttctcagtag aagtgcacaa
    17581 aacccttgcc cttgaagcct accgtgcttc cgtttacctc gctaaggaaa gaggcgcttt
    17641 caaaatctat gatcccaacc gcgagaaaaa caatccgttc attaaacgcc tgaaacaggc
    17701 tgaccctgct ctttacgaaa gcatggtcaa acatggaaga agaaatatcg ctctcctgac
    17761 cgtggcacct accggcacta caagtctcat gacacagaca acatcaggca tcgaaccagt
    17821 attttcactt ttctataaaa gaagaagaaa agtcaacccg aacgacaagg atgtaaagat
    17881 tactttcagg gatgaagtag gcgattgctg ggaagaatat cctgttctcc accataaatt
    17941 tatcgaatgg ctcaaagtca acaactatga tcctgacgcc gtgatgcaaa tgagcgatca
    18001 ggagattaat gaaattattg cccgttcacc ctattacagg tcaacagcca gtgatatcga
    18061 ctgggttgca aaggtgaaaa tgcagggcgc aatacagaaa tgggtcgacc attcaataag
    18121 cgtaaccatt aacctccctg ccgatgtaac agaagaactc gtaagtgagc tttatatgac
    18181 tgcatggaaa tcgggttgca agggtgtgac ggtttaccgt gaaggctcaa gaaacggagt
    18241 acttgtcaat gcaacaaaca accagggcac cgtggtagaa cggcccaaaa gacccaaagt
    18301 actcgattgc gacgtgataa gattcaaaaa caacgaagaa gactggattg ctttcgtagg
    18361 cctcaaagat ggaagacctt atgaaatctt tacaggtatg agcgatgaag atacactgct
    18421 cattcctaaa aatattgtga aaggtaaaat tatcaaggta aaagacgaag acggaaatac
    18481 cagatacgac ttccagtata ccgataaata tggatataaa aatacaatag gcggactttc
    18541 ccggatgttc aagcctgagt tctggaatta cgcaaaactt atctcagggg ttctccgcta
    18601 cggcatgcct ataccggatg tcgttaatct tatccagtca cttaaactgg acagcgactc
    18661 aatcaacaac tggaaaaacg gtgttgaaag agccctgaag cggtacatcc ccgatggtac
    18721 cagagctaaa ggaaaatgca aggattgcgg aagcgaaact ctggtctatc aggaaggttg
    18781 tttgatatgc aataattgtg gctcctcaaa atgcggtgga tcctgattca agatatttca
    18841 actcttaatt aatccaaatt cctttccggt gcttataaaa gcatcaatgg ctttatctag
    18901 ctgttctttt gtgtgagcgg cagagagctg cactcttatc cttgcctgac ctttaggcac
    18961 taccggataa tagaaaccta tgacataaat acccttttca agtagccttg ctgcaaaatc
    19021 ctgtgagagt tttgcatcat ataacataac ggcgcagata gccgactggg ttggctttat
    19081 tgtgaaacct gcctctttca tcttactgat gaaatattcc gcatttgcat gcagtctatc
    19141 ctgaagttcg tttgttgccg acatcatctc aaacatcttt attcctgctg cagcaaccag
    19201 aggaggaata gaattagaga aaagatatgg ccttgacctc tgccttagca tttcaataat
    19261 ctcttttctg cctgttgtaa agccacctat tgcccctccg aaagcctttc ccagagttcc
    19321 agttattatc tcaaccttgc ccataaggtt gaacaactct gtaactcccc tccccgtcct
    19381 gccaaccaca ccggcagaat gtgattcatc aaccatcacc agagcatcat atttctcagc
    19441 cagttcaaca atacggtcaa gaggtgcaac attaccatcc atcgaaaata caccgtcagt
    19501 cacaataatt ctgaacctct gcgcctgtgc cagttttagc tgtgcctcca ggtcctccat
    19561 atttgcgttt tcataacggt agcgctgcgc tttgcatagc cttacaccat caataatcga
    19621 agcatgattc aatgcatcgg atatgatagc atcctcttca gttagcagag gctcaaaaac
    19681 accaccattt gcatcaaaac atgccgcata aagtatggta tcctcagttc cgaaaaactc
    19741 agcaatcttc ttttcaagct tcttgtgaag gtcagtggtt ccacatataa atctgaccga
    19801 cgacatgcca taaccatgtg ttttcatcgc ctcttttgca gcttctacaa gctcagaact
    19861 gttcgacatg cccagataat tgttagcaca aaaattaaga accttctgac ctgtatcaag
    19921 agttatttcc ggtccctgcg gcgacacaat tattctctca tttttgtaaa ggcctgcctc
    19981 cttaatctct ttgatctgat tttcaagatg ttttttaaat gactgataca tattttttta
    20041 atttaaaatt aaatccaact aataaaccaa acttcaacca ttctcaatcc gccaaccagc
    20101 aaacccatca accagttaac cagtcaacca gctaacccgt aaacccgcta accagttaac
    20161 ccgtcaaccc ctaaaccagt taacccgtca accagctaac ccgtaaaccg ctaaccagtt
    20221 aacccctaaa ccagttaacc cgtcaacccg ctaaccctca attccagtcc attatcacct
    20281 ttccgcaatt accttcttcc ataacctcaa aagccttctg aaaatcatca acatgaaaac
    20341 ggtgagtgat taccggatta agatctatac cacctattat catcatctcc attttgtacc
    20401 atgtctccca catctctctt ccgtatattc ctttgatagt tattgccttg aagataatat
    20461 cactccaggg tatctcgcat acctgtggca gaattccaag cagggcaatg cttgacccat
    20521 tatacatgtt ttgaatcatt tcgcggaaag cagcaggaga acccgacatt tccaatccta
    20581 catcaaaacc tctcattccc agtttcttaa ctgcatcaga aatcttttca ccctttgaag
    20641 gattaaccgt gagtgtagca cccatctttt tcgcgatttc cagacggtaa tcgctcagat
    20701 cgcttgcaac aataaaccgt gcgcctgcat aacgcgctat agcaattgcc atactgccta
    20761 tcaatcctgc tcccgtaaca aggacatcct cgcccagaag cggaaaagag agagctgtat
    20821 gggttgcgtt gccaaaggga tccattatag ctgcccagtc atcaggtatt cttttgtcga
    20881 ggggtacaat gttcgaagcg ggaattttaa catattcagc aaatgctccg tcaatattta
    20941 ctcccactcc aaccgtgttc tcacaaacat gttctttacc tctccggcag ttgcggcaat
    21001 ggccgcatgc aatatgcccc tctccggtta ccctgtcacc ctcctttaaa tttatcacgc
    21061 ctgcccccat cttttctata taacccatat attcatgacc gataatcatg ggtgttttta
    21121 tggttctctg tgcccaatcg tcccagcgat aaatatgaag gtcggtaccg catatcgctg
    21181 tcttcttcac cttaatgatt acatcgttca ggccgggttc cggaacgggc acctcttcca
    21241 tccatattcc cctttcgggt tttgacttta cgagtgcttt catcgtcttc atacggaaat
    21301 aaatttaatt tgaaaaacag ggcgttattc tacggctaaa ttaaaataat tccggtgcta
    21361 ttatgctgat ttttgttatt atgttaaatg ttaacagctt taagtatctt tacgccggca
    21421 aaatcgtaaa gttattatgg taagagtcag atttgcacca agtcccaccg gaccgcttca
    21481 tattggcggg gtaaggacag ccctctataa ttatctcttt gcccgcaaac ataacggaac
    21541 ttttattctc cgcattgaag acaccgacca gacacgttat gtggagggag ctgaggaata
    21601 tattttcgaa tcactcgact ggtgcgggat taaagtggat gagggagtga gggaaggcgg
    21661 tccgtatggc ccctacaggc aaagcgacag aaaaaatata taccgccagt atgctgaatt
    21721 tcttattaat aaaggtcatg catattatgc cttcgatact cccgaagaac tagaaatgct
    21781 gcgtaaaaaa tacgagtctc agaaaaaaac atttctttat aacgccgaag taagaaacga
    21841 actaaaaaat tcactttcac tacctgaaca gcagtggaaa gaaatgataa acagcggcat
    21901 tccttatgtg ataagataca ggatgccggt gaatgaagaa atttcttttg atgatatgat
    21961 aagagggcac ataacggtaa actcatcaac actcgacgac aaagtgctct tcaaatcaga
    22021 cgggatgccc acctaccatc tggccaatat tgtagacgac cacctgatga aaattaccca
    22081 tgtaatccgc ggcgaagagt ggctgccttc cctccccctg catgtgatgc tctaccgttc
    22141 attcgaatgg gaagctccat tattcgctca ccttccgctt atccttaaac ccgacggaaa
    22201 aggcaaactc agcaaacgtg acggtgataa aatgggcttt cctgttttcc ctctgtactg
    22261 gccttacggt gaaaccgcaa ggggatacag agaagacggc tattatcccg aagcattcgt
    22321 aaacatgctt gctctgctcg gctggaaccc cgggacagaa caggagatct tcagcatgga
    22381 agaactgatt aaggaatttt cactggagag aatacataaa gccggctcac gcttcgaccc
    22441 tgtaaaagca agatggttca atcatgtctg gctccagaaa cgctcaaata accagctggc
    22501 acatgaattc agtgaaataa tcaaaggaaa gggttacagt tgcgatatcg ccagactgga
    22561 aacacttgtt ggactaataa aagaacgagt agattttgta aaggatatgt gggatgaagc
    22621 atattttttc ttcagcccgc cagaatcgta cgatagcgac gccctacgaa aaaaatggaa
    22681 cgaagagtca cctctcctcc ttgcagaagt atcaaattta ttaaggaaaa tagaagattt
    22741 tacccctgta aaccttgaaa aaaacgtcag ggaatggata gaaaagaaag gatgcaacag
    22801 tggtaccata atgcatgccc ttagaattgc agttgtgggt aaacttaaag gacccggaat
    22861 attcgacatc atcagctggc taggaaaaga cgaaacactt aacagaatag aaaaagctct
    22921 tgcaacaatc gttcaacagt catgaacctt cctcttgccg acgatttcat cgacatccat
    22981 aaccacggag cttcatctgc gacaggtatc ttctccgtag agaatattac tgccgatgaa
    23041 gaaagagttc cagacgattc acacggcctt gcatatacca tcggaataca tccctggcat
    23101 gtgaccgtaa aaaactacaa cgagcagctg aaaaaggtca ttctgtattc ccgccattcc
    23161 aatgtaattg ctttaggaga agcaggattc gaccgcctga gaggacctga aattaatttt
    23221 cagaaagaaa tctttgaaaa acaggttatt atttctgaag aggttaaaaa acctctatat
    23281 atacattgtg taaaggcctg ggaagaactt ctctcggctc ataaaatgct taaaccccat
    23341 atgccatggc ttgtacacgg tttcagaggc aagcatgaac tggcaatgca gttaatcacc
    23401 agaggaatgt acatttccct ctggttcgat tttgttatga gacctgaatc aactcaactt
    23461 cttaagtccc tcccctttaa cagaattttt cttgaaaccg acggagggga tacaaaaatc
    23521 agcactattt atgaaaaagt tgcaacagat cttgacctga ctgttgatca actaaaaagt
    23581 cagttatatt caaattttag aacttttttc ggagtctgat ggaagaatgg ctttcaagaa
    23641 ccgaactctg ccttggaaaa gaaaggcttg aaaagataaa atcgtcaaat attctggtgg
    23701 ttggactcgg tggtgttgga gctattgcag ccgagatgct ttgccgttca ggaataggaa
    23761 gaatgacgat cgctgacggt gacataatcc tgccctccaa ccgcaacaga caaattcacg
    23821 cactcatcag caccgaaagg caacccaagg cgcttgtgat ggaaaaacgt cttagagaca
    23881 ttaatcccga attacagctt acagttataa acgaatttat cagggatgag agaatgatcg
    23941 aaatacttga agaaccattt gattatgtgg tagatgctat tgacactctc tcacccaagg
    24001 ttttccttat atatcacagt ctaagaagga attaccgtat tataagctcc atgggttccg
    24061 gaggcaaatt cgatcctctg cttataaagg tcagtgatat atcagaaact tccttctgcc
    24121 atcttgcaag aatgatacgc aagaaacttc acaagctggg aataagagaa ggattcaagg
    24181 cagtatggtc acccgaaatt gttgaaaaat ccagaataat cagaacagag ggagaaagaa
    24241 acaaagcatc ggttgcagga acagtaccat ggatgccggc agccttcgga atagttattg
    24301 cttctgtagt tataagagac ctggcaggaa tagaaattaa cgcgtcagac atccaataac
    24361 aattgcatca ttcagccatc agattgcagc ctcttgtttc actgtggtca tacctcccca
    24421 atacttcaaa tcctccatca ctctggagcc ttccaatgtc gcctgttgct ataaaagaac
    24481 acgaatttat gtttgcaagg tcaataacat ttatagcacc cgctgtaccc ggtgctgcag
    24541 ataccgaaag aggatcgttc aactctctca caagtatctt catccatgga ggagaactga
    24601 atttgccttt gccctgtgac catgcctgac tcagaagctc tgtcatacca tattccgaat
    24661 gaatagcagg aacattgaaa ctctcgatga gtatattgtg aagctcttct cttgttattt
    24721 cttttcttct gcctttcatc cctcctgtct ctactattat tataccactc aagtcggaag
    24781 aaaatttttc agcaagatcc agcagtgcaa aacttactcc aatgagcatg acttttttgc
    24841 cctctcctgc cgcaagttta agtgtgtcta tcagcttcca tgaacctctt tcaaaaaatc
    24901 tgcttctttc agaagcacct gaatcaataa gtttatttac catgtaaatc agggaactgt
    24961 ttggccgttc aagataagag ggcaaaaggg ctgcaaacag gtaatccctg gggtcgccgt
    25021 aaaaaaactt gaagcctctc aaaaggctct catcataaag actggggtcg gtgatataat
    25081 gtcggctctt tatcgttcct gtggtaccgg tgctttcaaa aaccattacc gcctctctgc
    25141 ttccggtaat tacagtatga tttctgaaaa actcaaccgg caaaaaaggt atctcttcga
    25201 ttcgttgtac tccattaatt tcaatgccaa gcctttctgt aaattgtctg taaaccggat
    25261 tgtgctgtac ctgataatga aaaacttcaa tggcaatatc ttcaaattct tcagggtgtt
    25321 tgatattaaa aattctatct ctgtaccccg tattcatctc ggagggaaag tttttgtgcc
    25381 atcgggctga aatacccatt caaaggtgca ttccgcatat ggatcctgca tatctggcct
    25441 tttctccaga atggtgctta ttatcctgag cttggcatat cttgagtcac ttgtcctgta
    25501 tatccagatc tgattaatcc ttacagagtc accaatctct gtccacacgg gttcagtaaa
    25561 ctctgtaagt gcgttaaacg catcttctgc tgatttcgtg tcactatact gaccgtatct
    25621 gtagaatgaa tttttgaaat tattggttat aaaaattatt ttcagtggat taaaattcac
    25681 atcataatcc gacaatactg atataatatc aagcttgtta ttaaggtcag gaaccttttt
    25741 cccggttgga acatgaaagc cttcaatata ataggtaggt gatactccag ctctcttgtt
    25801 gtttatggtc ataataccag aatatacagg cttatcttcc ttcctgcatc ctgtcaaaag
    25861 tactgctacc gccactaaaa acagaaacag aggcctcatc tctttaaatt ttcacattat
    25921 cttctattgc aggaacaaat tttattcctc taagatactc tgataaaaag agtttatctc
    25981 tttgctaatc aaaatattac gaagcacaaa aattatatgg ttcca
//
LOCUS       DAOSNB010000005        25487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_1941_length_25487_cov_4.738440, whole genome shotgun sequence.
ACCESSION   DAOSNB010000005 DAOSNB010000000
VERSION     DAOSNB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_25487_cov_4.738440"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..988)
                     /locus_tag="PLG42_00580"
     CDS             complement(158..988)
                     /locus_tag="PLG42_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU29733.1"
                     /translation="MKRQRIMFQTLNFIILLTLSVTLFSQEKGKNNEFKPEVGQEGKD
                     VVWVPTPQALVEKMLDLAQVTPSDIVIDLGSGDGRTVITAAKRGARAIGVEYNPDMVA
                     LSYRNAEKEGLKNKVEFVNGDLFEYDFSKATVLTMFLLPDINLRLRPKILDMRPGTRI
                     VSNTFTMGEWTPDDTATIDDESTHWNTAYLWIVPAKVEGRWKFQPQGELVLRQEFQMV
                     SGELTTGGKKEAITGGKIRGDEITFYIGNTLYKGKIRGNSMEGTADNGKGTTRWTATR
                     "
     gene            1209..2735
                     /locus_tag="PLG42_00585"
     CDS             1209..2735
                     /locus_tag="PLG42_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU29734.1"
                     /translation="MNLKRREFIKLGGITAAGTIMIPPFLKSCRGPELSGKAESFMNH
                     FEVTVRQLEEVIRTAMSRGADYADLFFEHTLENYASLEDGKVDRAYSNVDFGVGIRVL
                     KGEQTGYAFSEDLNLEAMLKAAKTAASIADSQARVLPVNVSEYAVPDYYPVKVSWEDT
                     SVKEKIPFLQKVNDRIFELDKRVTKVNATIGDTTSYILFFNSEGRLVWDYRPMVFMYV
                     TCIMEKDGRIENFTSSRSSRQGYEFLTEELIETIANEAIGKTSLLFEAVKPKAGEMEV
                     VMSAGESGILLHEAIGHAFEADFNRKKTSIFSDKMGKKIAEPFINIYDDGTLLSNRGA
                     LNIDDEGNPTERTALVSEGILTSYLHDRISANFYKVKPTGNGRRQDFRNIPLPRMRCT
                     TMENGPHTREEIIASVKRGIYVDTFSNGQVNIGAGDFTFFVKSGYLIENGKLTRPIKD
                     INIIGNGPQALADIVMAANDSKLSDSTWTCGKGGQGVPVSMGLPTVKIKKLTVGGTNT
                     "
     gene            2757..4073
                     /locus_tag="PLG42_00590"
     CDS             2757..4073
                     /locus_tag="PLG42_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU29735.1"
                     /translation="MTREEKYSLAAAVIEHALSKGAQQVSVAIDESRSNEVEIRDRQI
                     DRLTESNRNSLTVRLYVDKKYSAHSTNRLKKDEVLKFTEEAIVATRFLAEDQYRTLPE
                     PELYYKGGGKTPDIFDPKLESVDPKSKIELAMQLLDEAYGKDERIISVTSYYSDSISS
                     NVLVTSNGFKGDSERTDISLYVTVSVKDKNGRPSDYWFESDLFFDRITKTGIGKKALE
                     RVLEKINPRKVSSGKYPMIVENRVASNLLSPVYNALQGYSIYQKQSFLAGKEGKKIAS
                     GKMHIEDDPTLPSEPGSRLFDEEGLAAVRRIIIENGILRNYYIDTYYGKKLGMKPTSG
                     SSSNIVFKKGDKDLPGLINSLKKGILVTGFNGGNCNGSTGDFSYGIEGFYIENGKVVH
                     PVNEMNITGNMNDLWFRLAEAGNDYIKGSSLKIPSLLIDDVDFSGI"
     gene            4149..5618
                     /locus_tag="PLG42_00595"
     CDS             4149..5618
                     /locus_tag="PLG42_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HOU29736.1"
                     /translation="MKYIKILMPFLIVSACAGPNGQPGNIKTETIYFNLEGKTLSVYT
                     TAKDTNLRLTLTGTMPFKKLESTSEREIYVFVDPSKTDQDFIGIGGALTDAAAETFAK
                     LPPAKQEEILQAYYSTDKGIGYTFARTNINSCDFSSGSYTYVKEGDKELNTFDVSHDR
                     QFKIPLIKKAIAAAGGKLTLFASPWSPPAFMKDNNDMLHGGKLKPEYFQSWALYYAKF
                     VKAYQKVGIPVWGITIQNEPMATQRWESCIYYAWEERDFLKNYLGPTMKKEGLGDVKI
                     IVWDHNRDLIIQRAQVIFNDPQASQYAWGMGFHWYEDWSGGEQMYRNVEYVHRFWPEK
                     HLIFTEGTPASFNPERYTSWRLGEDYGISMINDFNSGAEAWTDWNILLDEQGGPNHVG
                     NYCFAPVHADTRTGELIYTNSYYYIGHFSKFIRPGAKKILAASSRSMLLTTAFRNSDG
                     SIVVVVMNPTDRKAVYNLCAGLYYSEIESLPHSIQSIVF"
     gene            complement(5654..6520)
                     /locus_tag="PLG42_00600"
     CDS             complement(5654..6520)
                     /locus_tag="PLG42_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOU29737.1"
                     /translation="MKTKTTILICIILILNSAFLDGQKRTQMTAGEIIETIIRQTGSE
                     PVPNTVDVIKTGKPETQVTGIVTCMFAPMSVLRKAVELKCNLIITHEPVFYNHRDETS
                     QFSNDPVFLEKKKFIDDHGLVIWRFHDYIHRMKPDGIDYGMARKLQFDKYLVSGTDNR
                     FVIPETTLEGLLKHLKKVFPGHSFNVIGNLNMKVSKVTFSAGAPGSAAHFAMLRDPET
                     EVVIAGEVPQWETYEYVRDAILQGRNKAIIFLGHIPSEEAGMEYAAEWIKTFLTGIPV
                     HFVECQSSYFTY"
     gene            6626..8380
                     /locus_tag="PLG42_00605"
     CDS             6626..8380
                     /locus_tag="PLG42_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HOU29738.1"
                     /translation="MISRLFLSLLLVVACLVGSGLNGASSLKIDGDPPLLQKYNRTVL
                     GENVFVFEPGMDMKEVQAVIDTIFLRQSGRRSEFSNNRYALIFKPGEYNLDIRVDYYM
                     QVIGLGNSPEDVVIKGAVRSNTTHGNSVLTNFWRAVENMTIVPEEKSTMVWGVSQAAP
                     LRRVHIKGNLQLFDRGYASGGFLADSKVDGIITSGPQQQWLTRNTLFEGWEGGVWNMM
                     FVGVPQAPEENWPEKPYTVIGTTPVIREKPWLSYDKKGFCVKIPPIKKNSAGPGWING
                     EKPERIIYMNRFYIAKPGQDDAVSINAALKKGKNILFTPGRYYLNESLKVTQPGTLIM
                     GTGLATLIPEKGNSAIEIADVDGVIVCGLTFDAGPIFSEKLFVVGESGAGKSHQANPV
                     FLYDLFFRVGGHAQGSAGACLVINSNDVIVDHVWLWRADHGNGVGWEKNKCANGLIVN
                     GNNVIIYGLFNEHFQEYQTLWNGENGRVYFYQSEMPYDPPSVDAWKHNGIYGYASYKV
                     ADHVRTHEAWGLGIYNVFYDAPVIVDNAIETPEHLEKFIHNKIIFWLNGNKESVVKSI
                     INGKGGQVDVNNRKAAMK"
     gene            complement(8410..10272)
                     /locus_tag="PLG42_00610"
     CDS             complement(8410..10272)
                     /locus_tag="PLG42_00610"
                     /inference="COORDINATES: protein motif:HMM:NF003780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HOU29739.1"
                     /translation="MKKNFILLIYLLLCSFLFIPAINGQQKAIQTGKKAPSKIIVPVV
                     VNGEAQKIPEFENPDEWIREDLWVETEFDSDGDGRRDRMHVDVTRPRQTETEGIKLPV
                     IYESSPYFAGTGSTARQYFWNVRHELNSVPPPRTSMPPVQRVGKRPIISNSHVSTWVP
                     YGFIVVHSSAPGTGLSQGCPTIGTEIEALAPKAVIDWLNGRAKGYTTPDGNDEVKAYW
                     TTGKVGMIGTSYNGTIPFAAATTGVEGLEAIIPVAPNTSYYHYYRSNGLVRSPGGYLG
                     EDVDVLYEFIHSNPDRCAWCDSIYRDGIMARGMDRIKGDYNDFWAQRDLMNYMGKFRA
                     AVLMSHAFNDWNVMPSHSYRFIKVLKEKGVPLKIYYHQGGHGGEPPFSMMNKWFTRFL
                     SGVDNGVEKEPRAWIVRENDDRLNPTPYPDYPHPDARPVEFFLTAGAPQAGGLTTTKV
                     SGQGTETLVDNFSFSGSILAQAEWTEHRLLYLTPVLKEPVHISGVPSITIRAASSKPA
                     VNLSVWLVILPWNSARDAKITDNLITRGWADLQNYKSLRESEPLVPGKFYEMTFELEP
                     DDQIIPAGKQIGLMIFSSDREFTLWPDPGTKLTIDLDKTSIKIPVVGGAPALKF"
     gene            complement(10283..12721)
                     /locus_tag="PLG42_00615"
     CDS             complement(10283..12721)
                     /locus_tag="PLG42_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU29740.1"
                     /translation="MKSLQFSIIILLLTIISQSVSGQTRIIQELSEGWKFAKGENKGA
                     WQVNFDDSHWQNVVVPHDWAIEGPFNRDGDPNTGKLPWKDEGWYRIRLNIPSAWSGKQ
                     IYLLFDGVMAFPEVYINGELAGRWDYGYNSFYLNVTRLIKSGSENVVAVHADTRKHDS
                     RWYPGAGIYRKVSLIAVNPIHVDIWGTYITTPVVKPNYADVLIATTVNNCGAGTEEVV
                     LRHVIKNQSGSVVAEKEAKGIIAPGKNRIIETTVTLTDPHRWDINDPHLYSVETKVIN
                     NDKEVDNYVSTFGVRTIRFTADDGFYLNDRRVQLKGVCLHHDLGALGAAFNLRALERE
                     LEIMKEMGVNAIRSSHNVPAPELPWLCDRMGLLLFNEAFDKYDAKADITDTTDFENFA
                     HRNIRNFVVRDRNHPSVFIWSVGNEIGDVQRNVNNGFQRLHTMVNYVRKYDPTRPVTL
                     VCDNEQSASLRHFDYYDVHSWNYGRRYKLARQMEPNKAVIISESASTVSTRGFFELPL
                     PEKKTDFTKSLQVSSYDLNAPEWAEIPDDDFMWQQEEPYVAGEFVWTGFDYLGEPTPY
                     NSFFTRILGLTAADASRSSYFGIVDLAGIPKDRYWLYRSYWKTDANTVHIVPHWNWEN
                     MEGKNIPVFVYTNGDCAELFLNGKSLGKKCKNPKSENSVERFRLIWKDVIYEPGELRA
                     VAYKEGVKIGEAVVKTAGKPYQLRLTPDRTVIKSDGKDLSFILVEALDRNGNPCPLAN
                     NNFEIKVKGSGKFIGVDNGNPQSLTSFHSTKYDLFYGKAMIIVGASKTKGEITVEVIS
                     DGLKPAITRITTQN"
     gene            12842..13804
                     /locus_tag="PLG42_00620"
     CDS             12842..13804
                     /locus_tag="PLG42_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOU29741.1"
                     /translation="MINEIKITELEPEKFIQEKISEIRDAVGNGTAINALSGGVDSSV
                     VTMLGHRALGEKLRTIFIQNGLMREGEPESVVRIFRDLGVNVEVIDARREFIEALKGI
                     TDPEEKREAITQTFYKKVFGRIVRESGARFLLQGTILTDVDETVAGIKRQHNVFAQLG
                     IDPQEAFGYQILEPLIQLRKDCVRVVGRALGLPEEIFNRIPFPGPALAARIIGEVTEE
                     KLEVVRKATSVVEEILKETGAFQYMAILHNDRVTGMRGGKRDFGMQIEVRCWDSTDAR
                     TATPTRLSFEILERVAKSILEEVPGVVSVTYNIAPKPPSTIEAV"
     gene            13971..15200
                     /gene="serA"
                     /locus_tag="PLG42_00625"
     CDS             13971..15200
                     /gene="serA"
                     /locus_tag="PLG42_00625"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOU29742.1"
                     /translation="MARYSFDKNKIKVLLLEGIHDNALKHFNENGYYNVECYKEAFNG
                     TRLEDKIQDVHIIGIRSRTELRRDILQKARKLIAIGCFSIGTNQVDLYDAKMLGIPVF
                     NAPFSNTRSVAELVIAEIIMLMRGVPEKNALAHRKIWHKTIGNAVEIRGKTLGIIGYG
                     HIGSQVSILAESLGMNIIYYDIEKKLSLGNAKPVSSLEELLRNADVVTLHVPSTPLTK
                     NMISKEQVLMMKKGACLINTSRGDVVDYTAVAQGLKSGHLAGAAADVFDPEPSSNEEP
                     FIHELQDFDNVILTPHIGGNTREAQANIGLEVAEKLVKYSDTGSTIGAVNFVEISLMP
                     NNSTQRFLHIHRNVPGVLKEINNFFSSKGINISAEYLQTDADIGYVIIETESELDPSS
                     VEELKKIPYTIKVRMLY"
     gene            15303..15524
                     /locus_tag="PLG42_00630"
     CDS             15303..15524
                     /locus_tag="PLG42_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOU29743.1"
                     /translation="MPQIGKFLVIAGIILIIIGLILWLGGSRLNWIGHLPGDIRIEKE
                     NVKFYFPVTTMIVISILLSFILWVIRKLF"
     gene            complement(15539..17728)
                     /locus_tag="PLG42_00635"
     CDS             complement(15539..17728)
                     /locus_tag="PLG42_00635"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU29744.1"
                     /translation="MTQIQGKSYFATTVFLAFFEILTSQSAIHWETAVFNNDIWRYFV
                     GLSEPQQGWQQPGFDDSTWQIGPGGFGFGDNDDSTVIAQCSSVYLRIRFIISDTSRIN
                     SAVLHIDYDDAFVAYLNGIEIARAGINGINPPFNRTGDDHEAIMYRGGYPESFLIERS
                     VLKSVARPGENVLALQVHNNSSSSTDLSSNAWLSFGITGNSVLFRPVPSWFTPPVIFT
                     GSHLPVVVIKTQPGEVIRNEPKITADMKIIYRPGIINNITDSGNVYSGKVGIEIRGRY
                     SASLPQKPYGFETRDETGNNLNVSLLGMPEENDWILIANYNDKTFLRNLLAFELFRRM
                     GHYAPRTRFCEVIVNNNYQGIYLLCEKIKRDRNRVDISELKENENSGNDLTGGYIFKT
                     DYYTASDSWLSNYSPLNKPGAKVYFVYYYPEPDKITQYQKKYLKDFVNTFESILYSYD
                     YKDTRTGYRAYIDVNSFVDYFILSEVTRNVDAYKKSRYFYKDKDSKGGKIYSGPPWDY
                     DWAWRDIKENCIHFNQTDGSGWAYKINECNPNPVPPSWEVRLMQDPDFIRMIHDKYYL
                     LRKNILSMSQINMIIDSVATLLSEAQERHFQKWRILGINAGTPEYGEQPDTYEGEIIK
                     FKSWISRRLAWLDANMPGKSIIASQGYEPICRIFPNPADNYLHVQADTLISTIEIFSF
                     TGVPVKKSEGFNNYSATLNLEGLNEGIYYIRIRFRHGDIITRKLIKR"
     gene            complement(17880..18548)
                     /locus_tag="PLG42_00640"
     CDS             complement(17880..18548)
                     /locus_tag="PLG42_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29745.1"
                     /translation="MCFSAGASFAGSAMLAGIGVATLRKVSHPSQKLFASIPLLFAVQ
                     QFSEGILWLTLRANDYGRLQDTATYIFLLFALIIWPIMVPLSVIKMEKPGARKIILKF
                     LLATGVIVSSYYALCLLSFNVEPGIRDFHIEYRNNFPDKLALPAFILYTASTILPLFV
                     SGVKKMYIFGLLVLVSCIITGIFYREYLTSVWCFFAAIISAVIYYIITQEKWVPETEN
                     LRFQ"
     gene            complement(18619..19455)
                     /locus_tag="PLG42_00645"
     CDS             complement(18619..19455)
                     /locus_tag="PLG42_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29746.1"
                     /translation="MIKKHFKLFLCILIFASQLSGQVRNRDELARGVNFIETKIYSAE
                     EDARILELYKNLRVADVSDGLDMVGLPGSGLVDPAIHAAWTDPKDFKHVFRGIAITVR
                     YVPTQRPDRPAPGESFSQWESNAYNTITTEAFTGLIRPGSVIVIDDVEEKDIGSIGSN
                     NILNWYRLGAVGVVTDAGARDTDEIGLEGVPLYLRKKGRGIRPGRNEIESVNRPVSIG
                     GVLVCPGDVVVADGDGVVVVPRAVAEQVAKYANDILASDKAGRKSLYEKLGRPLDRTV
                     KE"
     gene            complement(19643..20263)
                     /locus_tag="PLG42_00650"
     CDS             complement(19643..20263)
                     /locus_tag="PLG42_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29747.1"
                     /translation="MGRNVKIIILSGLLLTSVAALYGQRGMRGWADTSSLKRPGREMR
                     MRQMPAMKEIPDSLRKGIGRGYRHGYGRIMPFDHGMWNMPYRRVPERFGPGWFYGPER
                     VPYTDPFRFGRRDFVRPVPPAMAPERPPMPYIYRIPDLTDKQKKEIESLREKFRDEMN
                     KFREENRRKMDEMRKSHLDKIKNLLTPEQKKWLEERIPQSTESKKI"
     gene            complement(20409..20819)
                     /locus_tag="PLG42_00655"
     CDS             complement(20409..20819)
                     /locus_tag="PLG42_00655"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOU29748.1"
                     /translation="MLYRFLDLFFIVFHTSLTLFNALGWIWKKTRLLNLITLFFTGAL
                     WLFLGLIVGTPGYCPLTDWHFDVLEKLGKTGLPNSYIKYLADRLTGFDFNPAFVDAMT
                     LIVFSAALLSSISFNLRDYFIRKRKGASSVNLNL"
     gene            complement(20896..21660)
                     /locus_tag="PLG42_00660"
     CDS             complement(20896..21660)
                     /locus_tag="PLG42_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29749.1"
                     /translation="MKKYIYLTIAPEALIGSMLPPEEFGCYMATGTKKSNKSQVIFFE
                     IDFEQIKSLINVDYLNRKCVAKPDGSPKSSVYLSIYRVLENIPLSSLLDLYLTTDTGK
                     VLQLKKGTYDPGKIGERKMFLYQELCPVSPQVASNLPPHEFLKILTDGSLQIRLPKLF
                     FVDLKLGELAINPLSGSAEHLPYGNISHLRDCLEIIKTEEMKLMKTVQREYTGALLYR
                     TIESGFYIGAVNDLIFYPYPTMAEIEKMNYEFFRAI"
     gene            22044..23552
                     /locus_tag="PLG42_00665"
     CDS             22044..23552
                     /locus_tag="PLG42_00665"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU29750.1"
                     /translation="MKKTTKIKRREFLGSAAAASVAFTVVPRYVLGGQGYVAPSDKLT
                     LGYIGCGTQGIREMAPLLAHPSIQIVAVCDPNKFSTNYIDWSMNSVRDTIRKALEEPS
                     WGENIKGIPGGRDLGQEFVQKYYAKIKKLPSYKGCSSYSDFRELLEKEKDLNAIKIMT
                     PDHLHACIAVAAMKKGKHVVTHKPIANRMHEWKVTYDMVRKSGVSTHLLAWSRRPGYN
                     VVKKMIADGFIGTLKEIHNWSNRPVWPQWQENPKESVPIPEGFDWQLWLGPVPDRPYH
                     PNYTHAVFRGWYDFGGGSIADMGHYSLFPLFLALGVNSPAVSAEAYGTTTCGFRENVA
                     VSIRNDVAFPLSCIVRFKFNAQGNLPPFELIWYDGGMKPLTPEELLAEGKSLPAEGMM
                     FVGDKGKILAGFRGENPTLIPSSRMKDFVPPPEEPEGLSGNDVWIDAFLKGTQSPGSF
                     IYAGPVTETINLGAVALRARKRVEYDSVNMRITNDEEANKFLTREYRKGWEM"
     gene            23674..24438
                     /locus_tag="PLG42_00670"
     CDS             23674..24438
                     /locus_tag="PLG42_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU29751.1"
                     /translation="MTKDDISLAQWALVDEIRAGKWKNLDFPAVARKDFGLNGIEFVN
                     TLFEVPTEGYLRQLKKNAENHNVTMVLIMVDDEGDGCSATREERRQFVINHRKWIDIA
                     AYIGCHAIRTNCRGPKDVNKEEALKWSVETYQMLLEYAVPARISVLIENHGGVSNDAD
                     WMLRLLKEVNNLYFGSYPDWRQPSADFDNVDYLTKMLPYAGGMSYRNQPTEELTAKMI
                     KLCREAGYRGWYGIESSGREEIKKGISLLKKYLNLS"
     gene            24643..25434
                     /locus_tag="PLG42_00675"
     CDS             24643..25434
                     /locus_tag="PLG42_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29752.1"
                     /translation="MMRIIALISFFCLYLPVGKATGKPDKLFSSEDILQLELRSDFTA
                     LQKNRMEEPVWFDGVLVYYQPEGTNLKIDVKVSVRGNFRLRPENCSFPPLLIDFRKES
                     VKNTLFEDQNRLKLVTPCQDEEDLLDEYIVYRLYNQVTEYSFRVRLAKILYFDTRTNE
                     PLFVKYSFFLEDEERMAKRNDAKVTEKFYTPFDLDYESYKKLSVFQYIIGNKDWYAVS
                     RKNIVIVQPSDTTMKPVAVPYDFDFSGFVGASYTKPDKLPAYSMG"
BASE COUNT         7492 a         4919 c         5532 g         7544 t
ORIGIN      
        1 aaggaaatcg tgaacatatg ctctttcttc aatacaaagc cctgtgtcct gcttcactag
       61 cagggcaaac agttccatca tggctttaag aatctcctca ctcatttatt ttaagtgtgc
      121 taaatttaaa agacagagcc agaagtcctg gccgggctta tcttgttgca gtccagcggg
      181 tcgttccttt tccgttatct gcggttcctt ccattgaatt acctctaatt tttcctttat
      241 aaagagtgtt gccaatataa aatgtaatct catccccgcg gatttttcca cctgttattg
      301 cctccttttt accaccggta gttagttctc ctgacaccat ctgaaattcc tgtcgaagga
      361 ccagttcgcc ctgcggctga aatttccagc gtccttcaac ttttgcgggt actatccaga
      421 gatatgcagt attccagtgt gttgactcat catcaattgt tgcagtatcg tcgggggtcc
      481 attcacccat agtaaaggtg ttggatacaa tacgggttcc gggtctcata tcaagaatct
      541 taggcctaag tcgcaggtta atgtcaggaa gaagaaacat agtcagaaca gtcgcctttg
      601 aaaaatcgta ctcaaaaagg tctccgttaa caaattcaac tttgttttta aggccttctt
      661 tttctgcatt tctgtaagaa agtgcaacca tgtcgggatt atattccaca ccgatggctc
      721 gggcaccgcg ttttgcagct gttattactg tacgaccatc gcctgagcct aaatcaataa
      781 caatatccga tggggtaacc tgggcaaggt caagcatttt ttccacaagt gcctgcgggg
      841 tcggaaccca tactacatcc ttcccttcct gcccgacttc aggtttgaac tcattattct
      901 ttcctttctc ctgcgagaaa agtgttacag atagagttaa taaaataata aaattcaggg
      961 tctgaaacat aattctctgt cttttcataa tgtagatgtt agatgaaaaa ttaataattt
     1021 attatccggg acaaaaggta taaaaaattc aatcaaagta taatatcata actgttaaat
     1081 atattttaat tgaataaaaa tttatttcta agtccagagt tattcagatt catctattct
     1141 atttcaatgc aaaattttgg aataataaat aatgtttcga aatttgagat cgaataaaaa
     1201 gtcataaaat gaatctgaaa cgccgtgagt ttattaaact tggcggtata acagcagcag
     1261 gtactattat gattccacct tttttgaagt catgtcgtgg tccagaactt tcaggcaagg
     1321 ctgaaagttt tatgaatcat tttgaagtta ctgtaagaca gctggaagaa gttattcgta
     1381 ccgccatgag cagaggggct gattatgccg acctgttctt tgaacacacc cttgaaaatt
     1441 atgcaagcct ggaagacggc aaagttgacc gggcatattc aaatgttgac tttggagtag
     1501 gtataagagt attaaaagga gaacagaccg gttatgcctt ctctgaggat ttaaacctgg
     1561 aagcgatgct gaaagcagca aaaactgcag caagtattgc cgatagccag gcaagggtat
     1621 tacctgtgaa tgtttctgaa tatgcagttc cggattatta cccggtaaag gtttcatggg
     1681 aagatacttc agtaaaagaa aagattccct ttttgcagaa agtaaatgac aggatttttg
     1741 aacttgataa acgtgtcaca aaagttaatg ccacaatagg agacactact tcttatatcc
     1801 tctttttcaa ctccgagggt cgtcttgtct gggattacag gcctatggtg tttatgtatg
     1861 ttacatgtat tatggaaaaa gacggaagga ttgagaattt tacatcctcc cggtcttcgc
     1921 gccagggata tgagtttctg accgaagagc tgatagaaac aattgctaat gaggctattg
     1981 gcaaaacttc attacttttt gaagcagtta agccaaaggc cggggagatg gaagtggtta
     2041 tgagtgcagg agaatcgggt attctgcttc atgaagctat agggcatgct tttgaggctg
     2101 actttaacag aaagaagact tctatttttt ctgataaaat gggcaaaaaa attgccgaac
     2161 cttttattaa tatttatgat gatggaacac ttctgtcaaa ccggggtgca cttaatattg
     2221 atgacgaagg aaatccgact gagcggactg cactggttag tgaaggaatt ctaacaagct
     2281 accttcacga ccgtataagt gcaaatttct ataaggttaa gccgacggga aacggaagaa
     2341 ggcaggattt cagaaatata cctcttccga gaatgagatg taccacaatg gaaaacggac
     2401 ctcatacaag agaggagatt attgccagtg tgaaaagagg aatatatgtt gatactttca
     2461 gtaacgggca ggtaaacatt ggggcaggtg atttcacatt ttttgttaaa tcaggttatc
     2521 ttattgaaaa tggaaaactt acacgtccaa taaaggatat taatattata ggcaatggcc
     2581 ctcaggctct ggctgatata gttatggctg ccaatgacag caaattatcc gattcgacct
     2641 ggacatgcgg taaagggggg cagggcgttc ctgtatccat gggtcttccc actgttaaaa
     2701 taaaaaagct aacagtagga ggaacaaaca catgaaaaac attaaaaatt aaagaaatga
     2761 ccagagaaga gaaatattca ctcgcagcag cagtaataga acatgcttta agcaaaggtg
     2821 cgcagcaggt atcggttgca atagatgaaa gccgaagcaa tgaggtggag atacgcgaca
     2881 ggcagattga ccgccttaca gagtccaaca gaaacagtct gactgtcaga ttgtatgttg
     2941 ataaaaaata ctcagcccat tcaaccaaca ggttgaaaaa agatgaggtg ctgaaattta
     3001 ctgaagaggc aattgtggca actcgttttc ttgcagaaga tcaatatcgg accctcccgg
     3061 aaccggaact ctattacaaa ggaggaggaa agactccaga tatttttgat ccaaaacttg
     3121 aatcggttga cccaaaaagc aaaattgaac ttgcaatgca gcttctcgat gaagcttacg
     3181 gaaaggatga aaggataatt tctgtgacat catattattc cgattcaatt agcagtaatg
     3241 ttctggtgac aagcaatggg ttcaaaggag attcagaaag aacagatatc tcgttatacg
     3301 tgactgtatc ggtaaaagat aaaaacggca ggccctctga ctactggttt gaaagcgatc
     3361 tgttttttga ccgtataaca aaaaccggga tcggtaaaaa agctcttgaa agagtactgg
     3421 aaaaaatcaa tccaagaaaa gtcagctcag gaaaatatcc gatgatagtc gagaacaggg
     3481 tcgcttccaa tctgctgtct cctgtttata acgcgctgca gggatacagt atttatcaga
     3541 aacagtcgtt tcttgccggt aaggagggta aaaaaattgc atctggaaaa atgcatatag
     3601 aggatgaccc aacactgcca tcagaacccg gctcgagact ttttgacgag gagggactgg
     3661 ctgcagtcag gagaataatt attgaaaacg ggatacttcg caattactat attgatactt
     3721 attacggtaa aaaacttggc atgaagccca catcaggtag ttcttcaaac atagttttca
     3781 agaagggtga taaggatctt ccgggactga ttaattctct caaaaaaggt attctcgtta
     3841 ccggtttcaa cggaggtaac tgtaatggct caacaggaga cttttcttat ggtatagaag
     3901 gattttatat cgaaaacgga aaggttgtcc atccggtaaa tgagatgaac attacaggca
     3961 acatgaatga tctgtggttt agacttgcag aagcaggaaa tgattacatt aaaggcagtt
     4021 ctttaaaaat accgtcactt ctgattgacg atgttgattt tagcggtatc tgaagtcagc
     4081 cttagaatac cctcaaaaga cttaataact aatattttag tttataaaaa tctgtaaata
     4141 gttatcatat gaagtacatt aaaattttaa tgccatttct tattgtttct gcatgtgcag
     4201 gtccgaatgg ccaaccgggg aatataaaaa ctgaaacaat ttactttaat cttgaaggaa
     4261 aaactctgtc ggtttataca acggcaaagg acacaaactt acggcttact ctaaccggga
     4321 ccatgccatt taagaagctc gaaagcacct cagaaaggga gatatatgtt tttgttgacc
     4381 cttcaaaaac tgaccaggat tttattggaa taggaggagc tcttactgat gccgcagcag
     4441 aaacatttgc aaaactgcct cccgcaaagc aggaagaaat tttacaggct tactacagca
     4501 ccgataaagg tataggctac acctttgcac gaacgaacat caacagctgt gatttctcaa
     4561 gtggaagtta cacttatgta aaagaaggcg ataaagaatt gaatactttt gatgtttctc
     4621 acgacaggca gttcaaaatt cctcttatta aaaaggccat agctgctgca ggcggaaaac
     4681 tgacactttt tgccagtccc tggagcccac cggcatttat gaaagacaac aacgacatgt
     4741 tgcacggagg gaagcttaaa cctgagtact tccagtcgtg ggcgctctat tatgcaaaat
     4801 ttgttaaagc ttaccaaaaa gtaggaatac ctgtgtgggg aataacaatt cagaatgaac
     4861 cgatggctac acagagatgg gaatcgtgca tttattatgc atgggaggag agggattttc
     4921 taaagaatta ccttgggcca acaatgaaga aggaagggtt gggagatgta aagattattg
     4981 tatgggatca taaccgtgac ctgataatac aacgtgcaca ggttatattt aatgatcctc
     5041 aagcttcaca atatgcatgg ggtatgggtt tccactggta tgaagactgg tcgggcggag
     5101 aacagatgta caggaatgtg gaatatgttc accgtttctg gccagagaag catcttattt
     5161 ttactgaggg tacgcctgca agcttcaatc ctgagagata cacctcgtgg agactggggg
     5221 aggattatgg catatcaatg atcaacgact tcaactcggg tgctgaggcc tggactgact
     5281 ggaatatatt gcttgatgaa cagggaggtc caaatcatgt aggaaattat tgttttgcac
     5341 cggtgcatgc tgatacaagg acaggcgaac tcatttatac gaattcatat tattatatag
     5401 gacatttttc gaaattcata cgtcctggag caaaaaagat tctggcagca tcaagcagga
     5461 gcatgctgct gacaacggct ttcagaaaca gtgatgggag cattgtggtg gtggtgatga
     5521 atccgaccga cagaaaagct gtctacaacc tttgtgctgg tttatattat tctgaaattg
     5581 aatccctgcc tcattcgatc cagtcaattg tgttctgatg cggatgagga tttaaacatt
     5641 tacgaatact ggctcaatat gtaaagtacg atgactggca ttccacgaag tgaactggaa
     5701 tgcctgtcag aaacgtttta atccattcgg ctgcatattc cattcctgcc tcttctgatg
     5761 ggatatgacc aaggaaaatt attgccttgt ttctgccctg tagtatggca tcacgtacat
     5821 attcgtatgt ttcccactgt ggtacttcgc cggctattac aacttctgtt tctggatctc
     5881 ttagcatggc aaagtgggct gcactacctg gtgctcctgc tgaaaatgtg actttcgata
     5941 ccttcatgtt caggttccca atgacattaa aagaatgccc tggaaaaact ttcttcaggt
     6001 gtttgagtaa gccttcgaga gttgtttcag gtatgacaaa tctgttatca gtaccgctga
     6061 caagatattt gtcgaattga agctttcggg ccattccgta atcaatgccg tcgggtttca
     6121 ttctgtgaat ataatcatgg aatctccaga ttaccagacc atggtcgtca atgaacttct
     6181 tcttttccag aaaaacagga tcgttgctga actgagaggt ctcgtcacgg tggttgtaga
     6241 aaaccggttc gtgggtaata ataaggttgc atttgagttc gacagctttg cgcagtacac
     6301 tcatcggagc aaacatgcag gttacaatac cggtaacctg tgtttcgggt ttacctgttt
     6361 ttataacgtc cacagtattg ggaacaggct cactaccggt ttgccgtatt attgtttcaa
     6421 ttatctcacc tgctgtcatc tgggtccgtt tctgaccgtc aagaaaggca gagttcagaa
     6481 taagaattat gcaaataaga atggttgttt tggttttcat gtttaaataa tttaattttc
     6541 ctcaaaatcc cgatttgata atcaaaatta ggaaaattaa taattaacat atgttttaaa
     6601 aaagaagaaa ctaaccgaaa atattatgat aagtagatta tttttatcac tgttgctggt
     6661 tgtcgcatgc cttgtaggca gcggcctgaa tggcgcatca agcctgaaaa ttgatggtga
     6721 tcccccatta ttacagaaat ataacaggac ggtccttggt gaaaatgttt ttgtgtttga
     6781 gcctggaatg gacatgaagg aggtacaggc tgtaatagat acaatatttc tgaggcagtc
     6841 gggccgaagg agtgagttca gcaataaccg gtacgctctg atattcaaac ctggagagta
     6901 caatctcgat atcagggttg attattacat gcaggttata ggattgggga attcccctga
     6961 ggatgtagtg attaagggag cggtaaggtc aaatacaact cacggtaaca gtgttcttac
     7021 aaacttctgg agggccgttg aaaacatgac aatcgtgccc gaggagaaat cgacaatggt
     7081 gtggggtgtg tcgcaggcag ctccactcag gagggttcat ataaaaggta accttcagtt
     7141 attcgacagg ggttatgcca gtggaggttt tctagctgat tcaaaagttg atgggataat
     7201 aacttccggt ccgcagcagc agtggcttac cagaaacact ctatttgaag ggtgggaagg
     7261 tggagtctgg aatatgatgt ttgtgggggt tcctcaggca cctgaagaaa actggcccga
     7321 aaaaccttat accgttatcg gtacaacacc tgtgataagg gagaaaccat ggctttcata
     7381 tgataaaaaa gggttttgtg ttaaaatacc cccgattaaa aagaactcag ctggtccggg
     7441 ctggattaac ggcgaaaaac ctgaacgtat aatatatatg aacaggtttt acattgcaaa
     7501 acccgggcag gatgatgctg tgtcaattaa cgcagcactt aaaaagggaa aaaacattct
     7561 cttcactccg gggaggtatt atctgaacga aagcttaaag gtaacacagc ccgggactct
     7621 aattatgggt accggacttg cgacccttat tcctgaaaaa ggcaactctg caatagaaat
     7681 agccgatgtt gacggggtta ttgtatgtgg tttgactttc gatgcaggac ctatcttttc
     7741 tgagaagctt ttcgttgttg gcgaatcagg tgcaggaaaa tcacatcagg caaaccctgt
     7801 gtttctttat gatctttttt tcagggttgg cggacatgct cagggttcag ccggcgcctg
     7861 ccttgttatt aacagcaacg atgtgattgt tgaccatgta tggttatgga gggcagacca
     7921 tgggaacgga gttggatggg aaaagaataa atgtgccaac ggcttaatcg ttaacggaaa
     7981 caatgtgatt atttatggtc tttttaacga acatttccag gaatatcaga ctctctggaa
     8041 tggtgaaaac ggcagggtgt atttttatca gagtgaaatg ccgtacgatc ccccgtctgt
     8101 tgatgcatgg aaacataacg gcatttacgg atatgcatca tacaaagtgg ctgatcatgt
     8161 aaggactcac gaggcgtggg gacttggaat atataatgta ttttatgatg ctccggtaat
     8221 agttgacaat gcaatagaga cccctgaaca tcttgaaaaa ttcatccata acaagataat
     8281 tttctggcta aacggcaaca aggaaagtgt ggtaaaaagc ataataaacg gcaaaggcgg
     8341 acaggttgat gtgaataatc gcaaagccgc aatgaagtga aaatagttag cttttgcctt
     8401 gaaggtttat taaaacttca acgccggggc tccgccaact acaggaattt ttattgatgt
     8461 tttatcaaga tctattgtaa gttttgttcc cgggtcgggc cagagagtga actcacggtc
     8521 gctcgagaaa atcatcaaac ctatctgttt acctgctgga ataatctggt cgtcaggttc
     8581 aagctcaaag gtcatttcat aaaactttcc aggcacgagt ggttcgcttt cccgtagtga
     8641 tttatagttc tggaggtcag cccatccacg ggttatcaga ttatcggtaa tctttgcatc
     8701 tctggcacta ttccatggta aaattacaag ccaaactgac aggtttacag caggtttact
     8761 gctggctgcc ctgatagtta ttgaaggaac acctgaaata tgcacaggct ccttaagtac
     8821 cggagtaagg taaagtaaac ggtgttctgt ccattctgcc tgtgccagga tactgccgct
     8881 gaatgagaag ttatcaacga gtgtttctgt tccctggcct gacacctttg ttgtagtcag
     8941 tccacctgcc tgtggagctc ctgcggtaag aaagaattcc acaggtcttg catcaggatg
     9001 tggataatcc gggtagggag taggattaag cctatcgtcg ttttctctga cgatccatgc
     9061 tctgggttct ttttccacgc cattatccac cccagataga aatcgtgtaa accatttgtt
     9121 catcatagaa aagggaggtt ctccaccatg cccgccctga tggtaataaa tcttcagagg
     9181 aacccctttt tcttttagta ctttgataaa ccggtaacta tggctgggca ttacattcca
     9241 gtcgttgaaa gcatgtgaca tcagaacagc tgctctgaat ttgcccatat aattcatcag
     9301 gtcacgttga gcccagaaat cgttgtaatc accttttatt ctgtccattc cgcgggccat
     9361 tatgccatcc ctgtaaatcg aatcgcacca ggcacacctg tcaggattgg aatggataaa
     9421 ttcatagaga acatcaacat cctctccgag ataacctccc ggacttctaa ccagtccgtt
     9481 ggagcggtag tagtggtaat atgaagtatt tggagcaaca ggtattatag cttctagtcc
     9541 ttctactcct gtagttgctg cagcaaaggg gatggtgccg ttataggatg tgccaatcat
     9601 tccaaccttg ccggttgtcc agtaagcctt tacttcatcg tttccatcgg gtgttgtgta
     9661 accttttgct cttccgttta gccagtcgat aacagctttt ggagcaaggg cttcaatttc
     9721 tgttcctata gttgggcagc cctgtgataa tcccgtaccg ggagctgagg agtgtaccac
     9781 aataaaacca tacggtaccc atgtggaaac atgagagttt gaaataatcg gtcgtttacc
     9841 tactctttga accggaggca tagaagtgcg tggtggtggt acagaattca actcatggcg
     9901 cacgttccag aaatactgac gggctgtact tccggtacct gcaaaatatg gactcgattc
     9961 atatataaca ggtaatttga taccttctgt ttctgtttgc cggggacgtg taacgtcaac
    10021 atgcattctg tcacgcctgc catcgccgtc tgaatcaaat tccgtttcaa cccagagatc
    10081 ctctcttatc cattcatccg gattttcaaa ctccggtatc ttttgtgctt cgccattaac
    10141 gacaacagga acgataattt ttgagggagc ctttttacct gtctgtatag ccttttgctg
    10201 cccattgata gcagggataa agagaaaaga acagagcagt aagtaaatga gtaaaataaa
    10261 atttttcttc attatgtaag atttagtttt gtgttgttat tctggttatt gcaggtttta
    10321 gtccgtctga aataacctct acagttattt caccttttgt ctttgatgca cccacaataa
    10381 tcatggcttt gccatagaaa agatcgtatt ttgttgagtg aaatgaagtc agcgattgtg
    10441 gattaccgtt atctacacct atgaattttc ctgaaccttt aactttaatt tcaaaattat
    10501 tgtttgcaag cgggcagggg ttcccatttc ggtcgagagc ttctacaagg ataaaggaga
    10561 gatctttacc gtctgatttg attactgttc ggtcaggagt cagacgcaac tggtatggtt
    10621 taccggcagt tttcacaact gcttcaccta ttttaacacc ttccttgtaa gcgactgccc
    10681 tgagttcgcc aggttcatat ataacatctt tccagataag tctgaaacgt tcaaccgagt
    10741 tttcggattt cggattttta cattttttgc cgagtgattt cccattcaga aacagttcgg
    10801 cacagtcgcc gtttgtatat acaaaaacag gaatgttttt gccttccata ttttcccagt
    10861 tccagtgcgg tactatatgt acggtgttag catctgtttt ccagtagctt ctgtatagcc
    10921 agtatctgtc ttttggtatt ccggcaaggt caacaattcc gaaatagctg cttcgtgagg
    10981 cgtcggcagc cgtcaggccg agtattcttg taaagaaact gttgtacggg gttggctcgc
    11041 caaggtagtc aaatcctgtc cagacaaatt cacctgccac atatggttcc tcctgctgcc
    11101 acataaaatc atcgtcgggt atttctgccc attcaggtgc attgaggtcg taagaactta
    11161 cctgtagcga ttttgtgaag tcggtctttt tttccgggag gggaagttca aagaatcccc
    11221 gtgtgcttac agtagaagct gattcactta tgatgactgc tttattgggt tccatctggc
    11281 gggcgagctt ataacggcga ccataattcc aggagtgaac atcgtaatag tcaaaatggc
    11341 ggagacttgc actttgctca ttgtcgcaaa cgagtgtcac cgggcgcgtc gggtcatatt
    11401 tacggacata attcaccatt gtatgtagcc tctggaaacc gttattaaca tttctctgaa
    11461 catcacctat ttcgttgcca acactccaga tgaaaacgga aggatgattc ctgtcgcgta
    11521 ctacaaaatt tcttatattt ctgtgggcaa aattttcaaa atcggtagtg tcggtgatat
    11581 cggctttagc gtcatattta tcgaaagctt cattaaacag aagcaatccc attctgtcgc
    11641 acagccatgg tagctccgga gcaggtacgt tatggctcga acggatggca tttacaccca
    11701 tttccttcat tatttcgagc tctctttcaa gtgctctcag gttgaaagca gcaccaagtg
    11761 caccgagatc atggtgcagg caaactccct ttagctggac gcgcctgtcg ttcaggtaga
    11821 atccgtcatc tgcagtaaat ctgatagttc taacaccaaa cgtggaaaca taattgtcaa
    11881 cctctttgtc attattaatc acttttgtct ctacactata cagatgaggg tcgttgatat
    11941 cccagcggtg cggatcagtc agagttacag tggtttcaat aattctgttt ttgcccggag
    12001 caataatacc ttttgcctcc ttttcagcca caaccgaacc tgactgattt ttaattacat
    12061 gcctcagtac tacctcttct gtaccggctc cacagttgtt aaccgttgtt gctatgagca
    12121 catcggcata attaggtttt acaaccggag ttgttatgta cgtgccccat atgtcaacat
    12181 gaatgggatt tactgcaata agactgactt tccggtaaat accagctccg ggataccatc
    12241 ggctgtcgtg cttccgggtg tcggcgtgaa cagccactac attttccgac cctgacttaa
    12301 tcaaacgagt cacattaagg tagaaggagt tataaccgta atcccatctt ccggctaatt
    12361 cgccgtttat ataaacttcc gggaatgcca taacgccgtc gaaaagtaga tagatttgct
    12421 tacctgacca cgctgaaggt atgtttaatc tgattctgta ccagccttcg tctttccatg
    12481 gcagtttgcc tgtgttagga tctccatccc tgttgaaagg cccttcaatt gcccagtcgt
    12541 gaggaaccac aacattctgc cagtgggaat catcaaaatt tacctgccag gcccctttgt
    12601 tttcgccttt tgcaaatttc cagccttccg aaagttcctg aataattctt gtttgacctg
    12661 aaactgattg tgaaatgatt gtaagcaata aaatgattat tgaaaactga agagatttca
    12721 tgtgttttta aattttactg gtgtaagctt caaagttatt aaaaacatca tcaaaatttt
    12781 tcaacaactc cgattttgat ctgtaatttt ggagtatgtt tataatataa aattttagat
    12841 tatgattaat gaaataaaaa ttacagaact tgagcctgaa aagtttattc aggaaaagat
    12901 ttctgagata agggatgctg taggtaacgg tacagctatt aatgcacttt cgggcggagt
    12961 tgattcatcg gttgttacaa tgctcgggca cagggctctg ggagaaaaac tcagaactat
    13021 attcatacag aacgggctta tgagggaagg agaacctgaa tcggtagttc gaatattcag
    13081 ggacctgggg gtaaatgtgg aagttattga tgcccgaagg gagtttattg aagctttgaa
    13141 aggtataaca gatcctgaag aaaagcgtga agccataacc cagacttttt ataagaaagt
    13201 attcggaagg attgtaaggg aaagtggtgc caggtttctt ttgcagggta cgattttaac
    13261 cgatgttgat gaaactgtag caggaataaa gaggcaacac aatgtgtttg cccagctcgg
    13321 gatagatccg caggaagctt ttggttatca gattctcgaa cctctgattc aattgcggaa
    13381 agattgtgtg agggttgttg gaagagcgct tgggctgcca gaagagatat ttaaccgcat
    13441 tccttttccc ggcccggcac ttgctgcccg tattatcgga gaggtaactg aagaaaagct
    13501 ggaggtggta cgaaaagcta catcggttgt tgaagaaatt cttaaagaga ccggagcatt
    13561 ccagtatatg gctattcttc ataatgacag ggtgacaggc atgcgcggtg ggaaaaggga
    13621 cttcgggatg cagattgaag tcagatgctg ggacagcaca gatgcccgta cagcaacccc
    13681 tacaagacta tcttttgaaa tacttgaaag agtggcaaaa tcaattctgg aagaagttcc
    13741 gggggttgtg tcagttacat ataatattgc acccaaacca ccttctacta ttgaagctgt
    13801 atgaaaatta tgtgatacca aattgaatga tatgttctta taagcatgca taaacttaaa
    13861 atatagttga ctaaaatata tttaaattaa aatatttaaa cagttaagaa caatatgttt
    13921 aaaatttaaa tcagaatata tatttttgcc ggataattaa aattgaagga atggcaagat
    13981 actcttttga caagaacaaa attaaggtat tgttgcttga agggatacat gataatgccc
    14041 ttaagcattt taatgagaac ggatattata atgttgaatg ttataaagaa gcatttaacg
    14101 gcaccaggct tgaggataaa atacaggatg ttcatataat aggcattcgc agccgtacag
    14161 aactgcgccg ggatatactg cagaaagcca ggaaactcat agctattgga tgtttcagca
    14221 ttggcacgaa tcaggttgat ctctacgatg caaagatgtt gggtatccct gttttcaatg
    14281 cacctttttc aaatacccgt tcagtggcag aacttgttat agcagagatt attatgttga
    14341 tgaggggagt gcctgagaaa aatgcccttg cccacaggaa gatatggcat aaaacgatag
    14401 gcaatgcagt ggaaataaga ggtaaaacac ttggtataat tggatacggc cacataggtt
    14461 cacaggtttc aatactggca gaaagtctgg ggatgaatat aatttattat gatatcgaga
    14521 aaaaactcag tctgggtaat gcgaaaccgg taagcagtct tgaagaactg ttaagaaacg
    14581 cagatgttgt gacacttcat gttccctcaa cgccacttac aaaaaatatg atttcaaagg
    14641 aacaggtatt aatgatgaaa aaaggagcct gtcttatcaa tacttctagg ggagatgtgg
    14701 tcgattatac tgctgtagca cagggattaa aatcgggcca tcttgctggt gcagcggctg
    14761 atgtatttga ccctgaacca tcttcaaatg aagaaccttt cattcatgaa cttcaggatt
    14821 ttgataatgt aatacttacg ccccatattg gaggaaatac acgggaagct caggcaaata
    14881 taggtctgga agtagctgag aaacttgtaa aatacagcga tacagggtct acaattggcg
    14941 ctgttaattt tgtagaaata tctcttatgc ccaataactc aactcagaga ttccttcata
    15001 tccaccgtaa tgtaccaggt gttctgaagg aaattaacaa ctttttttcc tctaaaggca
    15061 tcaacatttc agccgaatac ctccagacag atgccgatat tggatatgta ataattgaaa
    15121 ctgaaagcga actcgaccca tcttcagttg aggaacttaa aaaaattccc tataccataa
    15181 aggtaagaat gctgtactga taacatgtta gattaggttt ttggttaagc ttatttaagt
    15241 ttaagcaagg tatactgtcg ggttatattt atttcggtta attttacagc aaaaaagtga
    15301 atatgcctca gattggtaaa tttctggtta ttgcggggat aattctgatt attataggtc
    15361 ttatcttatg gctgggagga agcaggctga actggatagg ccatcttcct ggagacatac
    15421 gaattgaaaa ggaaaatgtg aaattctatt ttcctgttac caccatgatt gttattagca
    15481 ttcttctttc ttttatttta tgggtaatca gaaagctttt ctgacagact gtatagtttt
    15541 atctttttat tagtttcctg gtaataatgt caccatgcct gaagcgaatc ctgatgtaat
    15601 atataccttc attcaatccc tcaagattta aagttgccga atagttattg aatccttcag
    15661 attttttcac cggtacacct gtgaatgaaa agatttctat agtggagatc aaagtgtctg
    15721 cctgaacatg aagataatta tcggccggat tggggaaaat tctgcaaatt ggttcataac
    15781 cctgtgatgc tattattgat tttccgggca tatttgcatc gagccatgca agccttctcg
    15841 agatccagct tttgaattta attatttctc cttcatacgt atcgggttgt tctccgtatt
    15901 caggagttcc ggcattaatt cccagtattc tccatttctg gaaatggcgt tcctgtgctt
    15961 ctgacagcaa tgtggcaact gaatcaataa tcatatttat ttgactcatg ctcaggatgt
    16021 tctttctgag caggtagtat ttgtcatgta tcattcttat aaaatcaggg tcctgcatca
    16081 gacgaacctc ccatgatggc ggtactggat tgggattgca ttcatttatt ttataggccc
    16141 atccggaacc atctgtctga ttaaaatgaa tacagttctc ttttatatct ctccatgccc
    16201 agtcgtaatc ccatggtggg ccggaataga tttttccacc tttactgtct ttatctttat
    16261 aaaaatatct gcttttttta tatgcatcaa cgttgcgtgt aacctcactt agaataaagt
    16321 aatcaacaaa agagttcaca tcgatatagg ctctatagcc ggtacgggta tctttataat
    16381 cataactgta aagaatagat tcaaaagtgt ttacaaagtc tttcagatat tttttttgat
    16441 actgggttat tttatctggt tccggataat aatatacaaa atagactttt gcaccgggtt
    16501 tatttaacgg ggagtaatta ctcagccagc tgtcggaagc tgtgtaataa tcagtcttga
    16561 aaatataacc tcctgttaaa tcattacctg aattttcgtt ttctttcaat tctgaaatat
    16621 cgactctgtt tctgtctctt tttatttttt cacacagcag gtagattccc tgataattat
    16681 tatttactat gacttcacaa aatcttgtac ggggtgcgta atgacccatt cttctgaaga
    16741 gttcaaaagc aaggaggttt ctaagaaatg ttttatcgtt ataatttgca atcagaatcc
    16801 aatcattttc ttcgggcatt ccaagaagag acacattaag gttatttcct gtttcatcgc
    16861 gtgtttcaaa accataaggt ttctggggta atgatgctga atatcttccc ctgatttcaa
    16921 ttccaacttt gcctgaatag acatttccgg agtcagttat attgttgatt atgccgggtc
    16981 ggtatataat cttcatatca gcagttattt tgggctcatt tcttataact tccccgggtt
    17041 gagttttgat taccacaaca ggcaggtgtg aacctgtaaa tattacagga ggtgtaaacc
    17101 atgatggtac cggccggaat aatacggaat tacctgttat gccgaaagag agccaggcat
    17161 ttgaagagag gtcggtagac gatgatgagt tattgtggac ctgtaaagca agcacatttt
    17221 caccaggccg ggcaacagat ttcagcaccg acctttctat cagaaatgat tcgggataac
    17281 ctccccggta cataattgct tcatgatcat cacctgttct gttgaatgga ggatttatac
    17341 cgtttattcc ggcacgtgca atctcaattc cgtttaaata ggccacaaaa gcatcatcat
    17401 aatcaatatg aagcacagct gaattgattc gtgatgtatc ggatattata aatcttattc
    17461 tcaaatacac agaagagcac tgtgcaataa cggtactgtc atcattatca ccgaaaccaa
    17521 aacctccagg tccaatttgc catgtactgt cgtcaaaacc aggttgttgc cagccttgtt
    17581 ggggttcaga taatccaaca aaatatctcc atatgtcatt attgaaaact gctgtttccc
    17641 aatgaatggc ggattggctg gtgagtattt cgaaaaaagc caggaagaca gttgttgcaa
    17701 aatatgattt accctgtatt tgagtcatta ataataattg ttacaaggtt tttctatgtt
    17761 gttatttgcc attccggttt aattatgaga aaattctatt atcacttttt atcattaaga
    17821 tattgtgtat gatagatttt agttgtggct gtttaagcca tgaatattca ttccggccgc
    17881 tattggaaac ggaggttttc tgtttcaggc acccattttt cctgggtaat aatatagtat
    17941 ataacggcac ttattattgc tgcaaagaag caccagaccg atgtcaggta ttccctgtaa
    18001 aagatgcctg ttataataca tgaaaccaga acaagcaagc caaaaatgta catcttcttc
    18061 actcctgaaa cgaaaagcgg aagtattgtg gaagcagtat ataatataaa tgcaggcaaa
    18121 gcaagtttat cgggaaaatt atttctgtat tcaatatgga aatcccttat tcctggttct
    18181 acgttgaaag aaagcaggca gagggcataa tatgaagaaa caataactcc ggtagcaaga
    18241 agaaatttca gtataatttt tcttgcacca ggtttttcca ttttaatgac agaaagtggt
    18301 accattatgg gccatattat cagtgcgaaa agaaggaaaa tatatgttgc cgtatcctgg
    18361 agcctgccat agtcattagc tctcagagtg agccatagta tcccttcaga gaactgttga
    18421 actgcaaaaa gaagaggtat agaagcaaaa agcttttgtg acgggtggct tactttcctt
    18481 aaagttgcaa ctccgatgcc tgcaagcatc gcacttcctg caaaacttgc tccagccgaa
    18541 aaacacatat tagtttattt tatatgtcag gagtaaaagt aaatattttt caacagagtt
    18601 tttatttcac tgacaaattc actcttttac ggttctgtcg agtggacggc cgagtttttc
    18661 ataaaggctt tttctacctg ccttgtctga ggcgagaata tcatttgcat attttgccac
    18721 ctgttcggca acagcacgag gtaccacaac cacaccatcg ccatcggcta caaccacatc
    18781 gcccgggcaa accagaacac ctcctatgga gacagggcgg tttacagatt caatctcatt
    18841 tctgcctggt cttataccgc gacctttttt tcgcaggtaa agtgggactc cttcaagccc
    18901 gatctcatca gtatcccggg caccggcatc tgtcacaact cctacagctc ccagcctgta
    18961 ccagttgaga atgttgtttg atcctattga accgatatct ttttcttcga catcatcaat
    19021 cacaatcacc gagccgggtc ttattaagcc tgtaaatgct tcagtggtaa tagtattata
    19081 agcattgctt tcccattgtg agaagctttc acccggtgcc ggcctgtcgg gcctctgtgt
    19141 cggtacgtaa cgcacggtta tcgctatgcc cctgaaaaca tgcttgaaat ctttgggatc
    19201 ggtccatgct gcatgtattg cagggtcaac aagccctgaa ccaggtaaac ctaccatatc
    19261 gagtccgtca gaaacatcag ccacgcgaag atttttataa agttccagga ttcttgcatc
    19321 ttcttcggct gaatatatct ttgtctcaat gaaattcaca cccctggcaa gttcatccct
    19381 gttgcgtacc tgccctgaga gctgtgacgc aaatattaaa atgcacagaa ataacttaaa
    19441 atgctttttt atcatgtctt ttagattttt ggttaataat ccggatatta attttcctta
    19501 aaggtaagaa atacctaaaa aataaaaaat aaagagtgcc aattgaaggc accctttact
    19561 aacctgatct aacctaacct aacccaacct gacccttatt aacctgacct aacctgatgc
    19621 aattttataa acagtttgtt tcttatattt ttttgctttc tgtagattgt ggaatcctct
    19681 cttcaagcca ttttttctgt tcaggagtaa gaagattttt gattttatcc agatgggatt
    19741 ttcgcatctc atccattttc ctgcggtttt cttcgcggaa tttgttcatt tcatccctga
    19801 atttttcacg cagtgattct atttccttct tctgtttatc tgtaaggtcc ggtattctgt
    19861 aaatataagg cataggtggc ctctccggag ccattgcagg aggaacaggt ctaacaaaat
    19921 ctcttctgcc gaacctgaat gggtcggtgt aaggaactct ttccgggccg tagaaccaac
    19981 ctggtccgaa tctttcaggt actcttctgt agggcatatt ccacattccg tggtcaaaag
    20041 gcataatcct tccataacca tgcctgtagc ctctcccgat acccttgcgg agagaatctg
    20101 gtatttcttt cattgcaggc atttgccgca tacgcatctc cctgcccggc cgcttcaaag
    20161 aagaggtgtc ggcccaaccc ctcattccgc gctggccgta aagagccgct actgatgtca
    20221 gtagcaaccc gctgagtatt attattttta cattccttcc cataattcta agttttatca
    20281 aagtttcctc tattatcgtt ctctgctaat tagacaataa tattcacctg aaaattccct
    20341 taaacttcag aattaacaaa acattaacac aataaatagc cagcaggatt ttattctgtg
    20401 attggttatt ataaatttaa atttacactg ctagcccctt ttctctttcg tataaaataa
    20461 tctctaaggt taaatgatat tgatgacaga agagcagcac taaatacaat aagtgtcatt
    20521 gcatctacaa aagcagggtt aaagtcaaat ccggtaagcc tgtctgcaag atattttatg
    20581 taggaattgg gtaagccggt tttgcccagt ttttccagaa catcaaaatg ccagtcagta
    20641 agggggcagt atcccggcgt accaactatc aatccgagga aaagccataa ggctccggta
    20701 aaaaaaaggg ttataagatt caataacctg gttttcttcc atatccagcc cagggcattg
    20761 aaaagggtaa gggaagtatg gaatacaata aaaaacagat ccagaaaacg gtacaacatt
    20821 tacaatgtat gtccggtatt gactttaaaa cttcttatat acttatatta caggtaaaaa
    20881 gtgttgttat aatttttata tggctctgaa aaactcgtaa ttcatttttt caatctctgc
    20941 cattgtcgga tatggataga atatcaggtc gttaacggct ccgatataga aacctgattc
    21001 tatagttcta tataacaggg ctcctgtata ttcacgctga accgttttca taagtttcat
    21061 ttcctctgtc ttgattattt ccaggcagtc tctcaggtga gaaatgtttc cataaggcag
    21121 gtgttcagca gaacctgaaa gtggattgat tgcaagttct ccgagtttga gatctacgaa
    21181 gaaaagtttt gggagtctta tctgcagtga tccgtcagta agtattttca ggaattcatg
    21241 gggaggcaag ttactggcaa cctgggggct gacagggcaa agttcctgat agaggaacat
    21301 ttttctttct ccaatcttac cggggtcgta agtgcctttt ttaagctgga gaacttttcc
    21361 tgtgtcagtt gtcaggtaaa ggtcaagcag tgatgatagg gggatgttct ccagtactct
    21421 gtaaattgat aagtacacag agcttttggg tgaaccatcg ggtttggcta cacatttcct
    21481 gttcagataa tctacattaa ttagagactt tatctgttca aagtctattt caaaaaagat
    21541 aacctgtgat ttattcgatt tcttggttcc ggtagccata tagcaaccga attcttcagg
    21601 cggaagcatt gatcctataa gtgcttccgg ggcgattgtg aggtaaatgt attttttcat
    21661 aggtagatat tttatgttta aaaattattc agttctgctt ttcctccctg catcctttcc
    21721 cttcagctat tgagattccc atagaaccag gaggagtttt ttttgattgg cagattacgt
    21781 gtgtttcata tatagcagat ttacattttc ttattgtaat atggacagta ttaacttttc
    21841 gggtattaac tgcccctatt caaagataat aagtttgaat gccaaaagca aaagtttctg
    21901 tgtttaagag cacaaaaatg ttgctttctt ttctgactga atcagagcat ttttataatg
    21961 cattgtttgt atgattttat taaatttgag taattactga gttttaattt ttcatgataa
    22021 cttgaaactt aaaatctttc actatgaaaa aaacaactaa aatcaaaagg cgtgagtttc
    22081 tgggctcggc agctgccgct tcagtggctt ttactgtagt acccaggtat gttcttgggg
    22141 gccagggcta tgtggcaccc tctgacaagc tgacactggg ttacattggc tgtggtactc
    22201 agggaatcag ggagatggcc cctttgcttg cacacccgtc gattcagatt gttgctgtat
    22261 gcgacccaaa taaattcagc acaaactata tcgactggtc gatgaacagt gtacgcgata
    22321 ccatccgtaa ggctctcgaa gagccctcct ggggtgagaa tatcaagggt atccctgggg
    22381 gcagagacct tgggcaggag tttgttcaga aatattatgc aaagataaag aaattgccct
    22441 cctataaagg atgttcttcg tacagcgatt tcagggaact ccttgagaaa gagaaagatc
    22501 ttaatgcaat aaagattatg actcccgacc atctgcatgc ctgcattgca gttgctgcaa
    22561 tgaaaaaagg taagcatgtt gtgactcata aacccatagc aaacaggatg catgagtgga
    22621 aagttactta tgacatggtt cgcaagagtg gggtaagcac gcacctgctg gcatggagta
    22681 gaaggccggg ctataatgtt gtaaaaaaga tgattgccga cggattcatt ggaactctta
    22741 aagagataca caactggtct aatcgtccag tatggcctca atggcaggag aatcccaaag
    22801 agtcggttcc aattccggaa ggcttcgact ggcagctgtg gttagggcct gtaccagatc
    22861 gtccgtatca tcccaattat acgcatgcag tattccgcgg atggtacgac tttggcggag
    22921 gcagtattgc tgatatgggc cattacagtc tgttcccgct tttcctggca ttaggcgtca
    22981 attcccctgc ggtgagtgcg gaagcttatg gtactactac atgcggattc cgtgaaaatg
    23041 ttgccgtaag cataagaaac gatgtggcat ttcctctttc atgcattgta aggtttaaat
    23101 ttaatgcaca ggggaacctt ccaccttttg aacttatatg gtatgatggc ggaatgaaac
    23161 cgctcactcc tgaagaactt ttagctgagg gcaagtcatt accggcagaa ggtatgatgt
    23221 ttgttggcga taaaggtaaa atacttgcag gattccgtgg cgaaaatcca actctgattc
    23281 cttcctccag aatgaaagac tttgtgcctc ctccggaaga acccgagggc ctttcaggca
    23341 atgatgtatg gattgatgct tttcttaaag gaactcaatc acccggaagc tttatttatg
    23401 ctggccctgt tactgaaacc attaaccttg gtgcggttgc tctcagagca agaaagaggg
    23461 ttgaatatga ctctgttaat atgaggatta ctaacgacga agaagcaaat aagttcctta
    23521 caagagaata cagaaaagga tgggagatgt aaaatcgtat gttatgaata aactgggaag
    23581 aagagacttt attgaaaagt cagttctggc aggggctgca ggcatatttt ttcttctgga
    23641 cttacaaagc ctgagcccct tcctactctc agaatgacaa aagacgacat atcacttgca
    23701 caatgggcac ttgttgatga aataagggca ggtaaatgga agaatctcga ctttcctgct
    23761 gttgcaagaa aagattttgg gctgaacggg attgagtttg tcaacacatt gtttgaggta
    23821 cctacagagg gttatctcag gcagctgaag aaaaacgctg aaaatcataa tgttactatg
    23881 gtactgatta tggttgacga tgaaggggac ggatgttcag caaccaggga agagcgcagg
    23941 cagtttgtaa tcaatcaccg taaatggatt gatattgcag cttatattgg ttgtcatgct
    24001 atacgcacca attgccgcgg accaaaggat gtcaacaagg aagaagcact taaatggtca
    24061 gtggaaacat atcaaatgct gctggaatat gctgttccgg ccagaataag cgttttgatc
    24121 gagaaccatg gcggagtatc caacgatgcc gattggatgc tcagactgct aaaagaagtt
    24181 aataatcttt atttcggaag ctatcccgac tggaggcagc catcggccga ctttgataat
    24241 gttgattacc ttacaaagat gcttccttat gcaggtggga tgtcgtaccg gaatcaaccc
    24301 acagaggaac tgaccgcaaa aatgataaaa ctatgccggg aagctggata ccggggatgg
    24361 tatggcattg aatcgagcgg cagggaagaa attaaaaaag gaatatcact ccttaagaag
    24421 tatcttaacc tgagctgaag cgcaggttaa ttttgtgcac atgtggttaa gaagaaacaa
    24481 aaaataaacc ggcaatcatt tccgagcaac tgacataaag gagcataaag atttgaaatt
    24541 ttagaatggt ttaagaaata attttaattc ggaaagaagg aattttaacc gtgaacaaaa
    24601 cattcaggtg aatgtttatg gataaaatta ccagtgttcc ggatgatgag gattattgct
    24661 cttatatcat tcttttgcct gtatctgcca gttggtaaag ccacgggcaa acctgacaaa
    24721 ttgttcagtt cggaagacat cttacaactg gagctcagga gtgattttac tgctctacag
    24781 aaaaacagga tggaagaacc agtatggttc gacggagtac tggtttatta tcagcccgag
    24841 ggtacaaacc tgaaaataga tgtaaaagta agtgtgcgtg gtaatttcag actaaggcct
    24901 gagaattgtt cgtttcctcc tcttctaatt gatttcagaa aagagagtgt aaaaaataca
    24961 ctatttgaag accagaacag gttgaagctg gtaacgccct gtcaggatga agaggatctt
    25021 ctggatgaat acatagttta caggctttat aatcaggtta ctgagtatag tttcagggtc
    25081 aggcttgcta aaatattata ttttgatacc cggacaaatg aacctttatt tgtaaaatac
    25141 agcttctttc tcgaggatga agagaggatg gcaaagcgaa atgatgcaaa agtaaccgaa
    25201 aagttttata cgccttttga tcttgattat gagagttaca aaaagctatc tgtttttcaa
    25261 tatatcattg ggaacaaaga ctggtatgct gtttctcgta aaaatatagt tatagtacaa
    25321 ccttccgaca ccactatgaa gccagtggcg gtgccttatg attttgattt ttcggggttt
    25381 gttggtgcat catatactaa accggataaa ttgccagctt attcgatggg ataagttgtg
    25441 caactttttt tctgtaaaat catttaagcc acgattaatt ttttgac
//
LOCUS       DAOSNB010000006        24229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2089_length_24229_cov_4.171382, whole genome shotgun sequence.
ACCESSION   DAOSNB010000006 DAOSNB010000000
VERSION     DAOSNB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2089_length_24229_cov_4.171382"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..684
                     /gene="rpmH"
                     /locus_tag="PLG42_00680"
     CDS             529..684
                     /gene="rpmH"
                     /locus_tag="PLG42_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOU29753.1"
                     /translation="MKRTYQPSRRKRLNTHGFRKRMSTANGRRVLSARRAKGRKKLTV
                     SDEKRVK"
     gene            831..1940
                     /gene="mqnE"
                     /locus_tag="PLG42_00685"
     CDS             831..1940
                     /gene="mqnE"
                     /locus_tag="PLG42_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOU29754.1"
                     /translation="MKEFLTHIKDEFLRRTAEKVCEGERLSPAEGLLLYEKASLSLLA
                     MLSDTVRRRLNGDIVFFNCNFHIEPTNICINNCLFCSYRRKAGEPGSWEYSIDEMLET
                     VKKFDSKHVTEVHITGGVHPSRDIIFYCELIKRIHNHRPGLSIKAFSAIELDYMFSKS
                     GLSIEEGLKMLKECGLSSIPGGGAEIFDENIRNKICPGKTSSERWLAIHETAHRLGIP
                     SNATMLYGHIENYNHRIDHMERLRNLQDRTGGFNAFIPLKYKKHNNPLSYTGEVNILE
                     DLRNYAVARIFLDNFPHLKAYWPMLGKENARLSLSFGADDLDGTIEDTTKIYSMAGAA
                     ETNPSMSIEEITGLIRQSGYIPAERDTFYNIIREL"
     gene            2021..2875
                     /locus_tag="PLG42_00690"
     CDS             2021..2875
                     /locus_tag="PLG42_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOU29755.1"
                     /translation="MSLKEFLLSKIFFKNLGLAIAIVLGSIFLVLLWLNIYTRHGQAR
                     PVPDFYGLTLEETAKLAKKHKLRFQIMDSVYTNAVPRGCIAEQNPKPGYRVKKWRRII
                     LTINAFNPEMVEMPDLTGLPKRQALALLQTTGLEAGKLIYVPDLSVDVVLKQLYDGKE
                     IEKGQPVQKGSAIDLVLGKGLSNERTPVPNLIGMNYDRAKNSVLSASLNIGAYIFDNT
                     VKNRDDSTKAFVYRQSPEFNEEATLQLGSVVYIWLTTDSALLPVDSTLVSIPDSLIVT
                     SPGNSNDQ"
     gene            2882..4741
                     /locus_tag="PLG42_00695"
     CDS             2882..4741
                     /locus_tag="PLG42_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU29756.1"
                     /translation="MQKNILAYIALLLFYGNCINAQEVVTGLSRNIYLQANKSLSEST
                     KSTQSADTLELPFFDDFSDSYIYPDSKRWKDKFAFINNTYSVKQISAGVATLDALDSD
                     GRLYETASSFVFEADHLTSKPINLNYNSDDNIYLSFFYEAGGLSDKPEPGDSLVLQFW
                     APAEQEWFRIWKAPLNETNGFKPVIINIADSRFLKKGFRFRFINHASLSSAVSDPSMA
                     GNSDIWNIDYILLDKNRHPADTIPPDVAFTLPVRSVLKTYEEMPWNQFRQVFLSEMGP
                     YITINYRNNDRIVRNVTRSFQITDVYNNSVVHSFTAGATNIDSGAVVTYNAGLIYTFN
                     SSSTDSALFRIKCYLTTDAFDRKQNDTIVYYQRFGRCFAYDDGTSEGGYGISGLGSRN
                     AMLAYRFKSFVPDTLRAVQICFNDSYQNANQRAFDLMVWDNNKGIPGNVIYTQEDMVV
                     KQGESINGFYTYVLDKPVEIIGDFFVGWRQRSETFLNAGFDLNTPHNGRQLYYLNGEW
                     NVSQMSGSVMIRPVFGPEIKSSDINNPLYNEMRIKIWPNPASEYIYIGIGNQPLVSDI
                     GITIIDLAGRIILKSNYYNSDRLDISALPAGIYFVSASAKSKILGTTRLIISR"
     gene            4748..5779
                     /locus_tag="PLG42_00700"
     CDS             4748..5779
                     /locus_tag="PLG42_00700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU29757.1"
                     /translation="MSEEELTEYQELYEHHRFRVDAGQAPVRIDKYLATRIVNASRTR
                     IQYAAEAGNILVNNSPVKPSYKIKPGDIIQVLLPTPPREIELIPEDIPLNILYEDDDV
                     IVINKEAGMVVHPAYGNYTGTLVNALMWHFRDMPLFSTGELRPGLVHRLDKNTSGILV
                     VAKNEIAQNRLARQFFDRRTGRIYNALVWGTPDPPEGTITGHVGRNLKDRKIMQVFPD
                     GSHGKPAVTHYRVIESFGYVSHVECRLETGRTHQIRVHFAHIKHPLFNDAEYGGDKIL
                     RGTTFSRYQQFVKNCFELLPRQALHARTLSFDHPSTGKRMTFESDLPDDMKKVIEKWR
                     EYTSGKAEK"
     gene            complement(5799..6476)
                     /locus_tag="PLG42_00705"
     CDS             complement(5799..6476)
                     /locus_tag="PLG42_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOU29758.1"
                     /translation="MMKMIRKIAVFLTLISASGVLAGQENDFGIWLELNTTHKIVKNL
                     DATVNGELRTFNNSSQVRQVFIEGGLEYKLTKRFSVAGSYRLTSRREDDDNFYWRHRI
                     FADLKLKYPVNDFNISARLRFQRSTKTYIEDLEDLDAAYLYRLRMKIDYDIPKVPLAP
                     YIYYESFSPAFAGEGFRISKYRLSAGAEYHVTKKSDIQAGYIFQHDKDPKLRNTNILS
                     LGYAVKF"
     gene            complement(6460..7155)
                     /locus_tag="PLG42_00710"
     CDS             complement(6460..7155)
                     /locus_tag="PLG42_00710"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOU29759.1"
                     /translation="MEIQDISVLDSVNNSGIEFIEIHGFLEFLLRFSLNLAVIMILIR
                     WLYYTATRRKDYLFTYILIANIVFLLCFLLENVKLQVGFALGLFAIFGILRYRTDTIP
                     IKEMTYLFLVIGVSVINALTTSDTTLVEILFSNIVIILITFGLEKVWLLRHESSKLIT
                     YEKIDLLKPEKHEELLNDLRERTGIKNIKKVEIGKINFFRGTCDLKIFYETIEPEKVT
                     EKDNSRNEDDEDD"
     gene            complement(7325..8143)
                     /locus_tag="PLG42_00715"
     CDS             complement(7325..8143)
                     /locus_tag="PLG42_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU29760.1"
                     /translation="MNYIRYKLAALFIFLFFSAIYAQDVPDRPYPPRLVNDFAGLLTG
                     DEANALERKLVAFNDTTSTQIAIVIVKDLAGYAISDYAQRLGEKWGVGQKGFDNGIVI
                     VVKPKTLTSRGEVFIATGYGLEGAIPDLICADIVDNEILPAFREGDYFRGLDRATDVL
                     MLLASGEFSADEYRKKSKGNPETIPALILIIVIGLLMIIISGRSNHSHFSGRRSNLPL
                     WLLLSMMSSGSRSHRGSWGGFSGGGGIGGGFGGGGGFGGFGGGSFGGGGAGGSW"
     gene            complement(8143..8598)
                     /locus_tag="PLG42_00720"
     CDS             complement(8143..8598)
                     /locus_tag="PLG42_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOU29761.1"
                     /translation="MKASSFFTKEQKETILSAIREAEKDTSGEIRVHIETHCPEDVLD
                     RAAWIFRKLGMDKTRERNGVLFYLAVADRKFAVIGDAGINSKVPEGFWNQVKELLTKY
                     FKDGLFTEGLAEGIKLAGRQLKTFFPYRKDDVNELSDEISFETFSEKKN"
     gene            complement(8613..9215)
                     /locus_tag="PLG42_00725"
     CDS             complement(8613..9215)
                     /locus_tag="PLG42_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOU29762.1"
                     /translation="MKKGCLIGLIFGSLAVLFVIMMVLWGIRVYNNMVSMNEAVVSQW
                     GNVETQYQRRMDLIPNFVNTVKGAANFEQQTLTQVIEARSKATSVTVDPSNLSEEALA
                     RFQEAQGEISSALSRLMVVVEQYPEIKATQNFRDLQVELEGTENRIAVERRKFNEVAQ
                     SYNAYIKRFPNNFIASIGGFQPKGYFKSAEGAEKAPEVKF"
     gene            9431..12232
                     /locus_tag="PLG42_00730"
     CDS             9431..12232
                     /locus_tag="PLG42_00730"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU29763.1"
                     /translation="MKRFYLPVILLAIITLVACKKSEEVVISIPFEKYTLPNGLTVVL
                     HQDKSDPIVSVAIYYHVGSSRESRGKTGFAHLFEHMMFQRSENVGEDEFFRNIQSAGG
                     TLNGSTNQDRTNYYEVIPKNALELALWMESDRMGYLENTITKKSFAIQQNVVQNEKRE
                     SVDNAPYGFNNELILKYLYPEGHPYSWTVIGRMEDLANATVEDVKAFHRKFYVPNNAF
                     LVISGDFEPEQVKGLVEKYFGEIPSGEPVEKRDPMNVTLNSTVKLYHEDNFARAPRLT
                     MVFPTVERYSKDSYALNFLGEILAGSRKSPMYKVLVKEKKLTSNVMARNGSQELAGSF
                     TISVTANPGVNLTEVEKAIFESFERFEKEGFSEDDLKKIKAQYETRFVTQFASVQGKA
                     FTLAEYVMNTGDPGYYKKDLANIQAVTMDDIKRVYEKYIKGKNFVETSFVPKGQVNLI
                     AEGAVKADIVEEDVTKQTQVSIEGMPEEPVAKTPTKIDRSVKPPLGPDPEVTIPEPWK
                     SSLANGIRIWGIKHSELPIVSYSLVIEGGRLLDTPDKAGLANLVATLMNEGTKNKTPE
                     ELEDAIGLLGASINVRAGNEDITISVNTLTKNFEKTLALVEEMLLEPRWDEEQFALAK
                     SRIINTLKRNLASPDYLATRTLNRFIYGDNILAYDLSGTEESVASITIDDLKDFYNKY
                     FSPSVTRFLVAGDVDQQRVEAALAGLSNRWKPKEVVIPEFKIPPQPEKSQIIFVDVPG
                     AKQSVIAIGTPSIPRGHPDFHPATVANYKLGGSFNGIFNLILREEKGYTYGARSSLTG
                     GKNYGAFTASSRVRTDATFESVNIFKTEMEKYRENIPQDYVDFTKSALMKNNALRFET
                     LSALVDMLNTMSAYNLPEDYIKQEEAFIKELTADKILEYARKYIDPAKMYYVVVGDAA
                     TQLKPLEKIGFGKPLLMKF"
     assembly_gap    12353..12452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12453..12690)
                     /locus_tag="PLG42_00735"
     CDS             complement(<12453..12690)
                     /locus_tag="PLG42_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29764.1"
                     /translation="MSTDSTIRANKTILPYFKQPVLRVGPCPDLSGDSTIRANKTILP
                     YFKQPVLRVGPCPDLSGDSTIRANKTILPYFKQPV"
     gene            12665..12752
                     /locus_tag="PLG42_00740"
     tRNA            12665..12752
                     /locus_tag="PLG42_00740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12699..12701,aa:Leu,seq:taa)
     gene            13038..13895
                     /locus_tag="PLG42_00745"
     CDS             13038..13895
                     /locus_tag="PLG42_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU29765.1"
                     /translation="MQTVILNNGILMPMLGFGVFQITNPEVCERAVLDAIETGYRLID
                     TAASYMNESAVGNAIKRSGINREELFVTTKLWVQDTGYEKTKQAFERSLNNLQLDYID
                     LYLIHQPFGDVHCSWKAMEELYKSGKIRAIGLSNFSPDRVMDIIIFNEIVPAVNQIET
                     NPFNQQIKTQKFLKENNIQIEAWAPFAEGRNNLFKNNLLMSIAERYGKSVAQVVLRWL
                     IQRGIVAIPKSEKKERIKENFDIFDFELDSNDMKAIETLDSGKSCFFDHTDPEVVKWI
                     GSRKLYETK"
     gene            13930..14412
                     /locus_tag="PLG42_00750"
     CDS             13930..14412
                     /locus_tag="PLG42_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU29766.1"
                     /translation="MKKSIIVLFWMAMMLPFSSSFSQQPGLNQTTRSFTPEEQEIINL
                     SKQKWQWMSDKNADVLASLFHQKAVFVHMGGAWGTEQELNIIRSGGIWYKKADIHDVS
                     VNIIDNTAILLNRIDLLAVVGGNEVTNPFMVTEVYIKQNGKWTLGALSFTRLMTPGGQ
                     "
     gene            14639..16747
                     /locus_tag="PLG42_00755"
     CDS             14639..16747
                     /locus_tag="PLG42_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU29767.1"
                     /translation="MKTNSLFILLSLTLVIGISCSSRKYKELQPVLGTRSVGIIEKRG
                     LKFRDLNKNGKLDKYEDWRLTAEERSRDLLSKMSVEEKAGLMMINTLNMVGTRAAEAS
                     GGKLSASDLTEGAMPVQGGGQFSGREGSRERAGRTQGNRAGSSGMTGERIAGMGGTAG
                     TTKMVKEFHMRHFILRANESARVTAEWANKLQELCESEPLGIPALICSNPRNNITTNA
                     SLGTSVGTTVFSSWPGELGLSAMRDLELTREFADIARQEWVAVGIRKGYMYMADLATE
                     PRWSRVNGTFGENAEWVAKMIREIVLGFQGEKLNSSSVALTVKHFPGGGSGEKGQDSH
                     FEWGKKEVYPGGMFSYHLIPFQAAIDAGTSAIMPYYSLPAGTQYEEVGYAFNKGILTD
                     LLRNQMGFKGIINSDTGPLTGMPWGVENLTKEERYKKAIEAGVNIFSGESDPSMLVKL
                     IDSGIVDISYIDNSVFLLLKEEFELGLFENPYVDVDQAEKIVNNERFRERAALALRKS
                     IVLLRNENNFLPLKPKTKVYFESIQRNPRGSSSVAPNIYLVNDNKYDLEFVKTPGEAD
                     ILILWLQPTGNALFGSTGAPISLSLSKCGIDVDYVNKLTAKKPTVLVVNYTNPWVINE
                     VYNPKTKNIMGVLATFGTTADALLDVITGKFNPSGKMPFATPVSDEAVENQKEDVPGF
                     MEGENYALFKYDEGLSYPVR"
     gene            complement(17001..18347)
                     /locus_tag="PLG42_00760"
     CDS             complement(17001..18347)
                     /locus_tag="PLG42_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOU29768.1"
                     /translation="MDKRTVRLLFSSVAVFFIMLNVCAQDWPQVFGPNRNNISPQKGI
                     LRSWPEKGPEILWSVELGRGFGGPVVKDGKVYLLDREENQKDILRCLDFGTGKELWRF
                     EYEAPGSVMFPGSRSVPTVDGNYVYSVGHNGDLYCININTHKPVWHKNLMTDFGGARL
                     PIWAITQCPLIYGNMLIVLYSQDPYAGVVAFNKLTGDIIWKTDPIGNETYASPSIAKI
                     GGEDHIVVVFSSTNPIGHREVPRSMGKIVGFKPQTGEILWEYNNWENHIQVAPPLDAG
                     EGRIIVVGGYELGTAMIQVEKQAGGKYSVKEIFRHNDFGDHTKPPVLYNGYFYAQFST
                     NSKRDGLCCMSMDGKVMWKTMRNPSFDKGSMIFADGLIIASDGATKLYLIEPDPSGFK
                     PVATASLLSEGGAGSENDPVASRVAGSTQNWSPMALANGKLLLRDQRKLICVRVAK"
     gene            complement(18462..19919)
                     /locus_tag="PLG42_00765"
     CDS             complement(18462..19919)
                     /locus_tag="PLG42_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOU29769.1"
                     /translation="MERWLSGNGIDLNPHTITNTILKFYGKDYTFSVPILSMLTICGV
                     IPESCINNIPRLPFELAILPQRFYRFLNLGVVSYALPALIGVGIYLHRAGKRTYGLYG
                     KIRTLLIEPAIKKLEKLVPESGGFLEAIPLTGFVVMCLKAAGKSDNSIIEKGLEFLRR
                     QQREDGGWSVDTDLSTWITTLAIKAFGNDLRNQFDNMQIQVLRKHILSLQYSVKHPFN
                     GAKPGGWGWTNYSGSVPDADDTSGAILALLEIYSEAEEEKRAIIDGCKWLASLQNSDG
                     GFPTFCKGWGHLPFDSSCPDITGHAILASVRTKAVLSSYLTRKIRNKLDNCVNRAVKY
                     LEKNQSINGEWVPLWFGNQQTADKTNPVYGTAKVCTYLIDSLKFITSCTELSNRIRKM
                     IRRAQDYLIESQNPDGSWGGGKGVAGSVEETALTLCALAGENEEICSKGIEWLEKQDF
                     EHASPIGLYFALLWYYEKMYPLIFSTEAFRRIASN"
     gene            complement(19953..20246)
                     /locus_tag="PLG42_00770"
     CDS             complement(19953..20246)
                     /locus_tag="PLG42_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29770.1"
                     /translation="MEKEIILRKYYDLCQRLKSEVNSGGFWKGYLSTSALSTAVAIVA
                     LKVNGAPVDKERIKAGFDWLCKNINSDGGFGDTTNSLSNVSTTLLCYSAISYC"
     gene            complement(20252..21127)
                     /locus_tag="PLG42_00775"
     CDS             complement(20252..21127)
                     /locus_tag="PLG42_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOU29771.1"
                     /translation="MISLEKFLENTFKTHFFPDPKISEGRSIKAVLKKSLELLKDEVI
                     NDVRNFVLKMQHPSGGFIDRAGNPDMYYTLFGFFLADVLGLKENLQRTGKYAEDEISY
                     SMPEGVHLQCAAILLTELSDNRQIKNKIRRQIRRDVVMPHKEKNEYYAFVNLLASLYT
                     RDYIVLRQIYKLLSRLNQSDDLPVTVIAASLILYSTFKKPAEHLRQKLLDFYDNQGGF
                     RAVKTIALPDLLSTAVSLYALSYTGFDLLLIKPECLGFIDSHYINGGFCASVADRQTD
                     IEYTFYGLLALGSLA"
     gene            complement(21090..22682)
                     /locus_tag="PLG42_00780"
     CDS             complement(21090..22682)
                     /locus_tag="PLG42_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU29772.1"
                     /translation="MEKTVEKILPLVVPVEKQVRDEIRIKATLFTSEQNLLPPVTIDK
                     LEVIASEVLNKYGWDERYKAFVMVCCGNALWRPVVASIPYNRRLLLLPQCLKNSKKCV
                     AGEDEFGLLCGGCGNCNIPEYLQEAENLGYLTIVAEGTTIASKLIESGKIEAIVGVAC
                     MESLQKIFKTINSYFVPAIGVPLVFNGCTDTIADEDWLREELHYYANDNQFSLLNLHS
                     LKKGTISIFNTEQINKLLKLSDSTNDEIVKTLMLSGGKRMRPLLAILSYKAFKNNTDE
                     KLLKNLAMSIECFHKASLVHDDIEDEDSLRYGKKTIHAQYGVPIAINTGDFLIGEGYR
                     LISECDMDASDKVECLKIISEGHSAMAKGQGNELLARKNNILLPVNELLEIYKNKTGE
                     AIRVALLTGAIAGGADERVLKILSRFSYLFGIAYQLNDDLEDLKEVSSTSSLKNPNIL
                     ISILAESKDLNLREALHNAVSNNDFCLLRELIVNSNIKEETESLISMYINLIYECLAE
                     LNNITLKIALHDIVGKIFGKYL"
     gene            complement(22702..23865)
                     /locus_tag="PLG42_00785"
     CDS             complement(22702..23865)
                     /locus_tag="PLG42_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOU29773.1"
                     /translation="MSILKNLIKTDKRCLRIFLMNMVVKGILGFIRFRKRIKNGSMFP
                     AFQFISVTNNCNLHCQGCWVEVNGPKINIEPEKIDAVIRAGKKYGSYFFGILGGEPLL
                     YDGLFDIFEKHPDCYFQLFTNGTLLTKSTAQRLRRAGNVTPLISLEGDEYVADIRRGG
                     HRIYNRTVEAIETSVSEGLITGVAISVCKSNFEMAVSDEFVNMLHDSGVIYLWYYIYR
                     PSGSDPHYELALNTSEITKLRNFIVDARTRLPLILIDSYWNEKGIPFCPAIAGLSHHI
                     NPSGDIEPCPVIQLSCENLQYGEPGSLYENSLFLREFKEQLREKTNGCILMEDPAWLV
                     HFSENHKAKNTSNRENYLTSLLKAPSIPSHSSSPLILRKNYCIVLQRKQRFLV"
     gene            complement(23868..>24229)
                     /locus_tag="PLG42_00790"
     CDS             complement(23868..>24229)
                     /locus_tag="PLG42_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29774.1"
                     /translation="QCAEFCLFSVYEKKSEKVKVIFPQGCKNQCAACARICPSTAIIF
                     PKYKNGGAIGGADYFDENEELIRREADVKTFLGDDLVLALRKRQLKMNSIIREEASRK
                     AIEEREKFLLKNKEDQA"
BASE COUNT         7351 a         5073 c         4815 g         6890 t
ORIGIN      
        1 tgcaaattat gaaaaataaa aattcgagtc aaattttttt gacgaatgga gaataaacgt
       61 aaatgaacgg aggatttttt tagatgaatg aaaaaaggca gtctaaccag gaggaattga
      121 ccagcgatgt tgggttgggg gttcaagggt ttaaggttta aggtttaagg tttaaggttc
      181 aaggttcaag gttcaaggtt caaagggttc agggtttaag ggtttcaggg tttaagggtt
      241 tcaaggttta agggtttaag ggtttatgtt accaattgcg ccgggaacga agggttcgtt
      301 ctctattaaa cctccaggta cggcgcctgt gtttgtcatt gttccgtggc taaaaacatg
      361 ccgctcctgt cataggttaa aaaattaaat taaacctata aacaatttga tcaacaattt
      421 attaacgtta aatgtgtgag ttatttttac taaaaagtgt tgtatttctg aaaaaaataa
      481 tacatttgca gtctcattca gaaaaggtaa actcataaaa atcatacaat gaaaagaaca
      541 tatcagccat cgagaagaaa gaggttaaac actcatggtt tcagaaaaag gatgtcgaca
      601 gccaatgggc ggagggtgct ttcagccaga agggccaaag gcagaaagaa gctgactgtt
      661 tccgacgaaa aaagggttaa gtaagaaatt aaaaaaataa ccttataata aagggtactg
      721 ccagaaaaag cagcaccctt tattatttta ccaggattat cctgaatagc ccacgatgat
      781 ggaaaaagaa aacagccata aataatttgt aattttgacc accaattata atgaaagaat
      841 ttctgacaca tataaaagac gaatttctca gacgcacagc agaaaaagtt tgtgaaggag
      901 aacgtctttc acccgctgaa ggcctattac tttatgaaaa ggcatctctt tcattgcttg
      961 caatgctgtc tgacacagtc agaagacgtc ttaatggtga tattgtcttc tttaactgca
     1021 attttcatat tgaacctaca aatatttgca tcaacaattg cctcttctgt tcgtaccgca
     1081 ggaaagcagg cgaaccgggt agctgggaat acagtattga tgagatgctc gaaacagtga
     1141 aaaaatttga cagtaaacat gttactgaag tccatataac aggtggtgtc catccgtcaa
     1201 gggatataat tttttactgc gaactgataa aaagaattca caatcacagg cccggactta
     1261 gcataaaggc tttttcggca attgagcttg attatatgtt cagcaagagc ggtctgtcaa
     1321 tagaagaagg acttaagatg ctgaaggagt gtggactttc ctccattcct ggcggcggag
     1381 ccgagatttt tgatgaaaat atccgcaaca aaatctgtcc gggtaaaacc tcttccgaaa
     1441 ggtggctggc tattcatgaa actgcccacc gccttggaat accatcaaat gccaccatgc
     1501 tttatggaca tatcgaaaat tataaccacc gtatcgacca catggaacgc ctccgcaacc
     1561 tgcaggacag aacaggtggc tttaacgcct ttatccctct taaatataaa aagcataaca
     1621 atcccctctc gtacaccgga gaagtaaata ttcttgagga tcttagaaac tatgccgtgg
     1681 caaggatttt tctcgataac tttcctcacc tgaaagccta ctggcccatg cttggaaaag
     1741 aaaatgcccg gctcagtctc tctttcggag ccgacgacct tgacggcacc atagaagata
     1801 ctacaaagat atattcaatg gcaggggctg ccgaaaccaa tccttccatg agtattgaag
     1861 aaataaccgg actcatccga cagtcaggtt acattcctgc tgaaagggat actttctaca
     1921 atatcataag agaactctga cgagcagcat aatatgacac tgttatgttc caatataatt
     1981 ttatctttgc ctctgcgttt ttctaatgta aaccatcttt atgagtctga aagagttcct
     2041 tctgagtaaa atatttttca agaatctcgg acttgcaatt gccatcgtac tgggttcaat
     2101 ctttcttgtg ctgctgtggc tcaatatcta tacacgacac gggcaggcaa gacctgttcc
     2161 ggatttttac ggccttacac tggaagagac agcgaagctg gcaaaaaaac acaaactgcg
     2221 gtttcagata atggattctg tttatacaaa tgctgtcccg agaggctgta tagcagagca
     2281 gaacccgaaa cccggataca gggtgaaaaa atggagaaga attattctga caatcaatgc
     2341 tttcaatccc gagatggtag aaatgcccga ccttaccggg ctgcccaaaa gacaggcact
     2401 ggcgctactt cagactacag gactcgaggc cggtaaactc atctacgtgc cagacctctc
     2461 agttgacgtc gtactgaaac aactctacga cggcaaggaa attgagaaag gacaacctgt
     2521 ccagaaaggc tcagcaatcg atcttgtact cggaaaagga ctcagcaatg agagaactcc
     2581 tgttcccaac ctcatcggaa tgaattacga ccgggcaaaa aatagtgtgc taagtgcatc
     2641 acttaacata ggagcatata tttttgataa caccgttaaa aacagggatg attcaacaaa
     2701 ggctttcgtt tacaggcaga gtccggaatt caacgaagaa gccaccctgc agcttggctc
     2761 agtagtttat atatggctta caaccgattc ggcattactg cctgttgatt ccactcttgt
     2821 cagtataccc gatagtctaa ttgtaacatc tcccggaaac agtaatgacc agtaacccca
     2881 gatgcaaaag aatattcttg catacatcgc tcttctgtta ttctatggta actgcatcaa
     2941 cgctcaggaa gttgttaccg gactttcacg aaacatctat ctgcaagcta ataaatccct
     3001 atctgaatca actaaaagca cccaatcagc cgacacactc gaacttcctt tttttgacga
     3061 tttttctgac agttacattt accctgacag caaaagatgg aaagacaaat ttgcctttat
     3121 taataacact tattcagtga agcagatcag cgctggcgtt gcaacactcg atgcactcga
     3181 cagcgacgga agactctacg aaacagcatc gtcattcgta tttgaagctg atcatcttac
     3241 ttcaaaaccc attaatctga actacaattc tgatgataac atctatctga gtttctttta
     3301 tgaagcaggc ggattatcag acaaacctga accgggcgac agtctggtac tgcagttctg
     3361 ggcacccgct gaacaggaat ggtttcgcat ctggaaggcg ccactaaatg aaacaaatgg
     3421 ttttaagcca gttattatca atattgccga cagcaggttt cttaaaaaag gcttcagatt
     3481 cagattcatc aaccatgctt cactttcatc ggctgtctcc gatccgtcaa tggcaggaaa
     3541 cagtgatatc tggaacattg attacattct tcttgacaaa aacaggcatc ctgccgatac
     3601 catcccgccc gatgtggctt tcacactgcc tgtcaggtct gttctgaaaa cttatgaaga
     3661 gatgccctgg aatcagttca gacaggtatt cctttcagaa atgggtccat atatcactat
     3721 taactacaga aataacgaca gaatcgtcag aaatgtgaca cgcagcttcc agataaccga
     3781 tgtatacaat aatagtgttg tgcactcctt tactgcaggt gccacaaata ttgactcagg
     3841 tgccgtggta acatataatg cgggactaat atatactttc aactcatcca gtactgattc
     3901 tgcacttttc agaataaaat gctacctgac aacggatgca ttcgacagaa agcaaaatga
     3961 tacaattgta tattatcagc gtttcgggag atgctttgca tatgacgacg gcacatctga
     4021 aggtggttat ggtataagtg gactgggctc gcgcaacgcc atgctggcat acaggttcaa
     4081 atcatttgtt cccgatacgc tgagagctgt acagatatgc tttaatgaca gctatcagaa
     4141 tgccaaccag cgagctttcg acctgatggt atgggataac aacaaaggca ttccaggaaa
     4201 tgtgatttac acacaggaag atatggttgt taaacagggt gaaagtatta acggattcta
     4261 cacatacgtt ctagataaac ctgttgaaat aattggtgat ttctttgttg gatggaggca
     4321 aagatctgaa acattcctga atgcgggatt tgacctcaat accccgcata acgggcgaca
     4381 gctctattac ctcaatggag aatggaacgt atcgcaaatg tctggcagcg tgatgataag
     4441 gccagtcttc ggacctgaga ttaaatcatc agatattaac aaccccctgt ataacgaaat
     4501 gcggatcaaa atatggccca atcctgcttc ggaatatatc tacattggta taggcaatca
     4561 accgcttgtt tcagatatcg gcatcaccat aatagatttg gccggcagaa taatcctgaa
     4621 gtcaaattat tacaactccg acaggcttga tatttctgca ctgccggcgg gaatatattt
     4681 tgtcagtgct tcggcaaaat cgaagatttt gggtacaacc aggcttatta tttcaaggtg
     4741 agtgtctatg agtgaggagg aactgactga gtatcaggag ctttatgagc atcaccgttt
     4801 cagggtagat gcggggcagg cacctgttcg aattgataaa taccttgcaa caaggattgt
     4861 aaatgcatcc cgtacaagga ttcagtatgc cgccgaagca ggaaatattc ttgtaaacaa
     4921 ttcaccggta aaacccagtt acaagatcaa gccgggagac attattcagg tactccttcc
     4981 gacgccaccg cgcgagattg aacttatccc tgaagatatt cctctcaaca ttctttatga
     5041 ggatgatgat gtaattgtca taaataagga ggcaggaatg gttgtccatc cggcgtacgg
     5101 caattacaca ggaacccttg tcaatgccct tatgtggcat ttcagggata tgccgctttt
     5161 cagcaccggt gaattacgtc caggacttgt acacaggctt gataaaaaca cttcgggcat
     5221 tcttgttgtt gcaaagaatg aaattgcaca gaaccgtctt gccaggcagt ttttcgaccg
     5281 tagaactggc agaatataca atgctctggt atggggcacg cccgacccgc cggagggcac
     5341 aattacaggt catgtcggaa gaaacctgaa agacagaaag ataatgcaag tctttcccga
     5401 cggaagtcat ggcaaaccag cagttacaca ctacagggtg attgagagtt tcggttacgt
     5461 gtcgcatgtt gagtgccggc ttgaaaccgg ccgtacccac cagataagag ttcattttgc
     5521 acacataaag catcctttgt tcaacgatgc agagtacgga ggcgacaaaa tactcagggg
     5581 aacaacattc agccggtatc agcagtttgt aaaaaactgt ttcgaactcc tgccccgcca
     5641 ggctcttcat gcccgaacac tatccttcga ccacccttca acaggcaaaa gaatgacctt
     5701 cgaatcagac cttcctgatg atatgaaaaa ggtaatcgaa aaatggaggg agtatacttc
     5761 aggaaaagca gaaaaataat ttcacattca tagtccgttc agaatttaac agcgtagccc
     5821 agggataaaa tattggtatt ccttagctta ggatccttgt cgtgctggaa aatgtaccct
     5881 gcctggatat ccgatttctt tgtaacgtga tattctgctc ctgcagagag cctgtatttg
     5941 cttatgcgaa aaccctcccc ggcaaaagcc ggtgaaaaag attcataata aatatacgga
     6001 gccagtggca ctttgggtat atcataatcg atcttcatcc ttaatctata caaataggcc
     6061 gcatccagat cctccagatc ctcaatgtat gtttttgtac tcctctgaaa cctcagcctg
     6121 gcagaaatat tgaaatcgtt tacaggatat ttcagtttta ggtcagcaaa aatcctgtga
     6181 cgccagtaaa aattatcatc atcttccctt ctgcttgtca gcctgtagct cccggcaact
     6241 gaaaaacgtt tcgtcaattt atattccagt ccgccttcta taaaaacctg gcgtacctga
     6301 gaggaattgt tgaaagttct caactcacca ttgactgttg catcaagatt ttttacaatt
     6361 ttatgtgtag tattaagctc aagccagata ccaaagtcat tttcctgacc agctaaaact
     6421 ccggaagctg aaataagagt aagaaaaacc gctattttcc taatcatctt catcatcttc
     6481 atttcttgag ttatcttttt ccgtgacttt ctcaggttca atggtttcat aaaatatttt
     6541 cagatcgcaa gttcccctga agaaattaat ttttcctatc tctacttttt tgatattttt
     6601 tatgcccgtt ctttcacgga gatcattcag aagctcttca tgcttctcag gttttaacag
     6661 gtctattttt tcgtatgtta tcagctttga cgactcatgc cggagcaacc aaaccttttc
     6721 aaggccaaac gttatcagga ttataacaat attgctaaaa agaatttcca caagtgttgt
     6781 atcagatgtt gtcagggcat ttataacaga aacccctata acaagaaaca gataagtcat
     6841 ttcctttata ggaattgtgt ctgtccggta acgcagaatg ccaaatattg caaatagtcc
     6901 aagggcaaat ccgacctgaa gtttaacatt ttccagcagg aagcatagaa gaaagactat
     6961 gtttgcaatc aggatgtatg taaacagata gtctttacgt ctggtagcgg tataatacaa
     7021 ccatcttatc aaaatcatta tcactgcaag gttcagtgaa aacctcagca gaaactccag
     7081 aaaaccatga atttctataa attcaatacc tgaattattg accgaatcga gtacagaaat
     7141 atcctgaatt tccatattaa attgctcaat ataatcaact aatatttcag taatttcccg
     7201 ggttaatttt tatgattcca ggctccggtt atttactgaa acacgaagtt aaagtaatta
     7261 ttttaagatt taaaaagaaa ggtaaattaa aaaatcagga attatgtgta gaggatcggt
     7321 taaattacca gctacctccg gcacctccac caccgaagct tccaccacca aagccgccaa
     7381 agcctccgcc accgccaaat ccacctccaa taccacctcc tcctgagaat ccaccccagc
     7441 tgcctctgtg ggaacggctt cctgaactca tcatgctcag aagaagccat aaaggtaaat
     7501 tgctgcgcct gcctgagaaa tgtgagtgat ttgaacggcc cgaaataata atcattaaca
     7561 aaccgatgac aataattaat attagcgcag ggatagtttc aggatttcct tttgactttt
     7621 tccggtactc atcagctgaa aattctccgg aggcaagcaa cataaggaca tccgtggcac
     7681 gatcaagccc cctgaagtaa tcaccctccc tgaaggctgg gagtatttcg ttgtcaacta
     7741 tatcggcaca tataagatcg ggtattgcac cttcaagccc gtaaccagta gcaataaaaa
     7801 cctctcccct tgaggtcaaa gttttcggtt ttacaactat cacaattccg ttgtcgaatc
     7861 ccttttgccc caccccccat ttctcgccaa ggcgctgtgc ataatccgaa attgcatatc
     7921 ctgcaagatc tttgacaatg acaatggcaa tctgcgttga agtggtatcg ttaaatgcaa
     7981 caagctttct ctcaagggca ttagcctcat cgcctgtgag caaacctgca aaatcgttta
     8041 ccaaacgggg cggatagggt ctgtcaggaa cgtcctgggc atatattgca ctgaaaaaga
     8101 gaaaaataaa aagtgccgcc agtttgtatc ttatataatt cattaatttt tcttttccga
     8161 aaatgtctca aatgatattt cgtcgctcag ctcattaaca tcatctttcc tgtaagggaa
     8221 aaaagtcttc agctggcggc cagccagttt aataccctcg gcaagacctt cggtaaaaag
     8281 tccatcttta aaatattttg ttagaagttc ttttacctga ttccagaacc cctccggaac
     8341 ttttgagttg attcctgcat caccaataac ggcgaatttt ctgtcggcta cagcaagata
     8401 aaaaagcaca ccatttctct cgcgtgtctt atccataccg agtttacgga atatccaggc
     8461 tgctctgtcg agcacatctt caggacagtg agtttcaatg tgaaccctta tttcaccgga
     8521 tgtatccttt tcagcctccc ggatagctga taaaattgtt tctttctgct cctttgtaaa
     8581 gaatgatgat gctttcatgg tttacacccc tgttaaaatt ttacctcggg agctttttca
     8641 gcaccttcag ccgatttgaa gtaacctttt ggctggaacc cgcctatact ggcaatgaaa
     8701 ttattcggaa aacgcttgat ataggcattg taagactgag ctacctcgtt aaactttctt
     8761 ctctcaacag ctatcctgtt ttctgtacct tcaagttcta cctgcaaatc cctgaaattt
     8821 tgtgtggctt ttatctcagg atattgttcc acaactacca tcagcctgct aagtgctgag
     8881 cttatctctc cctgtgcctc ctggaaacgt gcaagtgctt cttcgctcag attggaaggg
     8941 tcaacagtta cagatgttgc cttcgacctt gcttcaataa cctgagtgag agtctgctgc
     9001 tcaaaatttg cagcaccttt tacagtattc acaaaatttg gaatcagatc catcctgcgc
     9061 tggtactggg tctcaacatt accccactga ctaactacag cctcattcat cgaaaccata
     9121 ttgttgtaaa ccctgatgcc ccatagcacc atcataatta caaaaagtac cgcaagagaa
     9181 ccaaaaatga gtcctattaa acaacctttt ttcatgataa taagagttta attccagttg
     9241 caaatttaga agaattctgc cggtttacaa ctcatacata agtataccgg tatgttttca
     9301 gcagaatacg gatgacaacc ggtaaccggc gattttcttt tttcctggca caatatatca
     9361 gatatctatt aaacaaatta tgctgtttca acaatatttg tattattttt agcctcaaaa
     9421 atctaccaaa atgaaacgtt tttacttacc tgttatttta ttggcaataa ttactttagt
     9481 tgcctgcaaa aagagcgaag aggttgttat atcaatccca tttgagaaat atactcttcc
     9541 caacggactg actgttgtcc tgcatcagga caaatcagat ccaattgttt cggttgctat
     9601 ttattaccac gtgggttcca gccgtgaatc cagaggtaaa accggtttcg cccacctttt
     9661 cgagcacatg atgttccagc gctcggaaaa cgttggtgaa gatgagtttt tcagaaacat
     9721 ccaaagcgct ggtggcacac ttaatggaag caccaatcag gacagaacca actattatga
     9781 agtaatccct aaaaatgccc ttgagctggc tctatggatg gaatcggaca ggatgggata
     9841 tcttgaaaat acgattacta aaaaatcatt cgccatacag cagaatgtgg ttcagaacga
     9901 aaaaagggaa agtgtcgaca atgcccctta cggattcaat aatgaactca ttctgaaata
     9961 cctctatcca gagggacatc catacagctg gaccgtaata ggccggatgg aggatctggc
    10021 aaatgctact gttgaagacg tcaaagcttt ccatcgaaaa ttttatgtgc caaataatgc
    10081 atttcttgtt atttctggcg attttgagcc agagcaggtg aaggggcttg tggaaaaata
    10141 tttcggagaa ataccttcag gcgaacctgt cgagaaaaga gatcctatga acgttaccct
    10201 caactcgacc gtaaaacttt accacgaaga caattttgcg agggcacctc gtcttactat
    10261 ggttttcccc acggttgaaa gatattcaaa agactcttat gctctgaatt tcctgggcga
    10321 aatcctggca ggctcaagaa aatcacccat gtataaggta cttgtaaaag agaaaaagct
    10381 tacttcaaac gttatggcaa ggaacggttc acaggaactg gcaggcagtt ttacaatatc
    10441 ggttactgcc aatcctgggg ttaacctcac cgaggtggaa aaagcaattt ttgagtcatt
    10501 tgaaagattt gaaaaagaag gattttctga ggatgacctg aaaaagatta aagcacagta
    10561 cgaaacccgc tttgtgactc agtttgcgag tgtacaggga aaagctttca ctcttgcaga
    10621 atatgtcatg aatacgggcg atccgggata ttataaaaag gaccttgcaa acatccaggc
    10681 agttactatg gacgacataa aaagggtcta tgaaaaatat ataaaaggca aaaacttcgt
    10741 tgaaaccagt tttgttccga aaggacaggt taatctgatt gccgagggtg ctgtcaaagc
    10801 agatattgtg gaagaagatg ttaccaaaca gacacaggtt agcattgaag gcatgcctga
    10861 agaacctgtt gcaaaaactc ccaccaaaat tgaccgtagt gtaaaaccac ccctgggtcc
    10921 cgatcccgaa gtgacaattc ctgaaccatg gaaatcatca cttgcaaatg gtataaggat
    10981 atggggtata aaacattccg aactgcctat tgtcagctat tcgctggtaa tagaaggtgg
    11041 acgtcttctc gatactcccg ataaagcggg cctggcaaac cttgttgcaa cactcatgaa
    11101 tgaaggaacc aaaaacaaga ctcccgagga acttgaagac gctatagggc tattaggtgc
    11161 atcgataaat gtgagagcag gtaatgagga cattacaata agtgttaaca ctcttaccaa
    11221 aaacttcgaa aagacccttg cactggttga ggaaatgctt ctcgagccaa gatgggatga
    11281 agaacagttt gcactggcca aaagcagaat aataaacact cttaaacgga atcttgcaag
    11341 tccggattac cttgctacac gcacacttaa tagattcatt tacggtgata atattcttgc
    11401 ctatgacctt tcaggaacag aagagtctgt tgcatcaata acgattgacg atctgaagga
    11461 tttctacaat aaatatttct caccttcggt tacccgcttc ttagttgcgg gagatgttga
    11521 tcagcaaagg gtcgaagctg ctcttgccgg gctcagcaac agatggaagc cgaaggaggt
    11581 ggttattcct gaattcaaaa ttccgcctca acctgagaag tcgcagatta tttttgttga
    11641 tgtgccaggt gcaaaacagt cggtaattgc tattggcaca ccatccattc caaggggtca
    11701 tcccgacttt catccggcaa ctgttgccaa ctacaaattg ggtggctctt ttaatggaat
    11761 tttcaacctg atactgcgcg aggagaaggg ctacacctat ggtgcaagat cttccttgac
    11821 aggaggcaag aactatggag cttttacggc ttcatcccgc gttcgcacag atgccacctt
    11881 cgaatcagtg aacatcttca aaactgaaat ggaaaaatac cgtgagaata ttcctcagga
    11941 ctatgtagat tttacaaagt cggcccttat gaaaaacaat gctctccgct tcgaaacact
    12001 aagtgctctt gttgatatgc taaatacaat gagtgcatac aacctgcctg aagattacat
    12061 aaagcaggag gaagcattta ttaaggagct gaccgctgac aaaatactgg aatatgcccg
    12121 taagtatatt gacccggcca agatgtatta tgtggttgtg ggagatgctg ccacacagct
    12181 taaacctctt gaaaaaatag gattcggcaa acctctgctt atgaaatttt aaagattttt
    12241 gtagtatctt tgtaacgcac attctgcagt tggcctgcag tagtatgtca tgatgcgttg
    12301 cccggatggt ggaatctccc gataaatcgg gacagggtcc gacacgcagt acnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggaccggt tgtttgaaat aaggaagtat
    12481 tgttttgttt gcccggatgg tggaatctcc cgataaatcg ggacagggtc cgacacgcag
    12541 gaccggttgt ttgaaataag gaagtattgt tttgtttgcc cggatggtgg aatctcccga
    12601 taaatcggga cagggtccga cacgcaggac cggttgtttg aaataaggaa gtattgtttt
    12661 gtttgcccgg atggtggaat cggtagacac gcaggactta aaatcctgtg gccagttaaa
    12721 cggctgtgcg ggttcaagtc ccgctccggg tacaaattga ttaaatgcca gtgaaatcgg
    12781 ttgttaatct gtaataatca gatattctaa agaggtttta cattaactgt acgacttacc
    12841 attatcctga ttcgtaaaag tattatacct gaaatttcta cctgaaaaga tatcattaga
    12901 atagaaagga ttaattattt cgccataaat tctctaattc tcgttttaat gaaagtcatc
    12961 gaacttttta aaatttgagt tattataatt gcatattaaa ttgagaaaca ttgtcaaaaa
    13021 attctttaaa aacataaatg caaactgtaa tattaaacaa tggtatttta atgcccatgt
    13081 tgggttttgg ggtctttcag ataacaaacc ctgaggtatg cgaaagggcg gtgctagatg
    13141 ctattgaaac tggatacagg ttaattgaca ctgccgcatc atatatgaat gaatcagccg
    13201 ttggaaatgc gattaaaagg agcggaataa atagggaaga attatttgtt accaccaagt
    13261 tgtgggttca ggataccggt tacgaaaaaa ctaaacaggc ttttgaaagg tctctgaaca
    13321 accttcaact tgattatatt gatttatatt taatacatca gccttttggt gatgtgcatt
    13381 gttcatggaa ggcaatggaa gagttgtata aaagcggaaa aatcagggct attggcctca
    13441 gtaatttttc ccccgaccgt gttatggata ttataatttt taatgaaatt gtccctgcag
    13501 taaaccagat tgaaacgaat ccatttaatc agcagattaa aacacaaaaa tttctgaaag
    13561 aaaacaatat tcagattgaa gcatgggctc cgtttgctga agggagaaat aatcttttta
    13621 aaaataatct acttatgtca attgccgaaa gatacggcaa aagtgtagcc caggtagttc
    13681 tcaggtggct tattcagaga ggaattgttg caattccaaa atcggaaaaa aaagaaagaa
    13741 taaaggaaaa tttcgacatc ttcgattttg aacttgacag taacgacatg aaggctatag
    13801 aaacccttga ttcggggaaa agttgttttt ttgaccacac ggacccagaa gttgttaaat
    13861 ggataggaag tagaaaactt tatgaaacca aataatttaa aaattttaac cttaaaaatt
    13921 atcatcgata tgaaaaaatc gattattgta ttattttgga tggcaatgat gttgcctttt
    13981 tcatcatcat tcagccagca gccaggttta aaccagacaa ccagaagctt taccccggag
    14041 gaacaggaaa ttatcaatct ctctaagcaa aagtggcagt ggatgtcaga caaaaatgca
    14101 gatgtactgg ccagcctgtt tcatcagaaa gctgtatttg ttcacatggg aggcgcatgg
    14161 gggacagaac aggaacttaa tatcatcaga agcggaggaa tctggtacaa aaaagctgat
    14221 atccatgatg tatctgtaaa tattatagat aatacagcta tacttcttaa cagaatagat
    14281 cttctggcag tggttggtgg aaatgaagtt accaatccgt tcatggttac tgaagtatat
    14341 atcaaacaga atggcaaatg gacactggga gctctttctt ttaccaggct gatgacaccg
    14401 ggcgggcaat aataaacgat atcaactgct ctattgtgat caagtaaaga ataagcgggt
    14461 aagaaaaagc catgtcttca ttcctcaggg atatatgcaa taatgttagt atagtgcggt
    14521 cacaataatg attatgatgt gatatttttt ttaccttgta agaataagtg agtaataaaa
    14581 tttttaaact tacataatct cttacaaact ttttgaaatt ctatttatga taaaaaaaat
    14641 gaaaacaaat agtttattta ttcttttatc actgacactg gtaattggaa tatcatgtag
    14701 tagcaggaaa tataaagagt tgcagcctgt tcttggcaca aggtcggttg gaataatcga
    14761 aaaaagaggc ctcaaattca gagatctcaa taaaaacggc aaacttgata aatatgaaga
    14821 ctggcgtctt actgccgaag agagaagcag ggatcttctt tcaaaaatgt cggtagaaga
    14881 gaaggcaggt ttaatgatga ttaacactct taatatggtt ggcacaaggg cagcggaggc
    14941 ttcgggagga aaactttcgg caagcgacct caccgaaggt gctatgccag tccagggggg
    15001 tggccagttt tcaggcaggg aaggaagcag ggaaagggca ggaaggacac agggtaacag
    15061 ggctggcagt tccggaatga caggagaacg tatagccgga atgggtggaa cagcaggaac
    15121 aacaaaaatg gtgaaagagt ttcatatgcg ccattttata ctgagggcta atgagagcgc
    15181 gcgagtcaca gccgaatggg caaataaact gcaggaattg tgcgaaagcg aaccgctcgg
    15241 aatccctgca ttaatttgct caaaccctcg taacaatatt accaccaacg cttctctcgg
    15301 gacaagtgtt ggcacaactg ttttttcttc ctggcccggt gaactcggac tttcagcaat
    15361 gcgtgatctt gagcttacaa gagaatttgc agatattgcc cgccaggagt gggtggctgt
    15421 gggaatcaga aaaggctata tgtatatggc cgaccttgct acagagccaa gatggtcgag
    15481 agttaacggc accttcggag aaaatgcgga atgggtagct aagatgataa gggaaattgt
    15541 cctcggtttt cagggtgaaa aactaaattc ttcgtcggtg gcactcacag ttaagcattt
    15601 ccccggtggc ggttcaggcg agaaaggtca ggattcacat ttcgaatggg gtaaaaaaga
    15661 agtatatccc ggcggcatgt tcagttacca cctgataccc ttccaggctg caatcgacgc
    15721 aggtacatca gcaatcatgc cttattattc acttccagca ggcacacaat atgaagaagt
    15781 aggctacgca ttcaataaag gaattcttac agacctcctc cgtaatcaga tgggatttaa
    15841 aggaatcata aattcagata caggtccact aacaggcatg ccatggggtg ttgaaaacct
    15901 tacaaaagag gagcgctata aaaaagcaat agaagcaggt gtaaatattt tttcaggaga
    15961 atctgatcct tcaatgcttg tcaaactgat agattctgga attgttgaca tcagctatat
    16021 agacaattct gtatttctac tgttaaaaga agaatttgaa cttggactgt tcgaaaatcc
    16081 atatgttgac gttgatcaag cagaaaagat tgtaaataat gaaaggttca gagaaagggc
    16141 tgctctggct ttaaggaaat ccattgtcct gctaaggaat gaaaataact ttctgccact
    16201 aaaaccaaaa actaaagtat attttgaatc tattcagaga aatccgaggg gttcctcatc
    16261 agttgcccct aatatatacc tggtgaatga caataaatat gatctggaat ttgtaaagac
    16321 accgggtgaa gcagatatcc ttatattatg gctgcaaccc acaggaaatg ctttattcgg
    16381 gtcaaccggc gcaccaattt ctctatcctt gtcgaaatgt ggtattgacg tcgactatgt
    16441 taataagtta acagcaaaaa agccaacggt gctcgttgtc aattacacta atccatgggt
    16501 aatcaacgaa gtttataacc caaaaacaaa aaacatcatg ggtgtccttg caacctttgg
    16561 aacaactgca gatgcattgc tggatgttat aactggcaag tttaacccca gtggcaaaat
    16621 gccctttgcc actccggtat ctgatgaagc agttgaaaat cagaaggagg atgtgccagg
    16681 atttatggaa ggtgaaaatt atgcactctt taagtatgat gaaggtttaa gctaccctgt
    16741 cagatagtta ttcacgaact gataaaacag acctattaag agtcctcttt attttatgct
    16801 taatcagtaa aacatacctg ctctccgaag cctaatttac tgtatcatgc gtgtttttcg
    16861 tttagctgac tccggagagc aggtaaaacc tttcattctc aataacctga taaaggatat
    16921 cagaatttaa atacgaatta agagtcccgt cttaagttgg gacagtatcc atcaaccagc
    16981 atcttgaaaa tcttcctgaa tcacttagca actctcacac atattaattt tctctgatcc
    17041 ctcagcagta gcttgccatt tgcaagtgcc atcggagacc agttctgtgt tgagcctgct
    17101 actcttgagg caacagggtc attttcagaa cccgctcctc cttcgctgag caatgaagct
    17161 gtcgcaacag gtttaaaccc tgacgggtca ggttcaatca ggtacagttt cgttgctccg
    17221 tcggaagcaa taattagtcc atctgcaaat atcatgctac ctttatcgaa tgaaggattg
    17281 cgcatcgttt tccacatcac ctttccgtcc atgctcatgc agcaaaggcc atcgcgttta
    17341 ctgttggtag aaaactgtgc atagaaataa ccgttgtaca atactggagg ctttgtgtgg
    17401 tctccgaagt cattatgcct gaaaatctct ttaacactgt atttgccacc ggcctgtttc
    17461 tcaacctgaa tcattgctgt accaagttca tagccaccta ccacaattat tcttccttca
    17521 cctgcatcaa gaggaggcgc cacctgtata tgattttccc agttgttgta ttcccagagg
    17581 atctcaccag tctgtggctt aaatccaaca atcttaccca ttgacctcgg gacctccctg
    17641 tggcctattg gatttgttga tgaaaacaca actactatat gatcctcacc acctattttt
    17701 gcaatagagg gacttgcata agtttcattc ccgataggat ctgtcttcca gataatatcg
    17761 cctgtaagct tattgaaggc aacaacaccg gcatagggat cctgtgaata caatacgatg
    17821 agcatgtttc cgtaaataag agggcattgt gttatagccc atataggcaa tctggcaccg
    17881 ccaaaatcgg tcatcaggtt tttgtgccat acaggtttgt gtgtgttgat gtttatacag
    17941 taaaggtcgc cgttgtgacc aactgaataa acataatttc cgtcaacggt ggggacgctc
    18001 cgtgaaccgg ggaacattac agatccggga gcttcgtatt caaatctcca gagttctttt
    18061 cctgtaccaa aatccagaca gcgcaagata tctttttgat tttcttccct gtcgagaaga
    18121 tagactttgc cgtcttttac aaccggccca ccgaatcccc tgcccagttc tacactccac
    18181 agaatttcag gtcccttttc aggccatgag cgtagtatgc ctttctgcgg tgagatgtta
    18241 ttcctgtttg ggccaaaaac ctgtggccag tcctgagcgc aaacatttag cattataaaa
    18301 aatactgcaa cagaagaaaa cagtaatctg acagttcttt tgtccataac ttcagtaatt
    18361 taaattaaca tacacaaatt taaggatcta aaccttcatg ttaaattctt ttatcactca
    18421 aaaggaagaa tcaattgttt atcacgaaaa agaaagccgc attaattact tgctattctt
    18481 ctaaaagctt cagtactaaa tatgagggga tacatttttt cataatacca gagcaatgca
    18541 aaatagagtc ctatgggaga agcatgctca aaatcctgtt tttcaagcca ttctatgccc
    18601 ttgctgcata tttcttcgtt ttcccctgca agggcacata aagtaagtgc tgtttcctct
    18661 accgaacctg caactccttt ccctcctccc caactcccat cagggttctg agattcaata
    18721 agatagtcct gggctcttct tatcattttc ctgattcggt tgctcaattc agtacaggat
    18781 gtaataaatt taagagaatc aattaaatat gtgcatactt tggcagttcc ataaaccggg
    18841 tttgttttat ctgctgtttg ctgatttccg aaccagagag gtacccactc tccattaata
    18901 gactgatttt tctcaagata tttaactgcc ctgtttacgc aattatcaag tttgtttctt
    18961 attttacggg ttaaatatga tgataaaacg gccttagtcc tgactgaggc caggatagca
    19021 tggcctgtaa tgtcggggca gctgctgtcg aagggaagat gtccccatcc cttgcaaaat
    19081 gtcggaaacc caccatcaga attctgcaag ctggcaagcc atttacaacc atcgatgata
    19141 gctctcttct cctcttcagc ttctgaatag atttcaagta atgccagtat tgcacccgag
    19201 gtgtcatctg catccggtac cgaaccagaa tagttcgtcc aaccccatcc accgggcttc
    19261 gctccgttaa aaggatgttt cacactgtat tgcagcgata aaatatgttt tctcagtact
    19321 tgaatctgca tattatcaaa ctgattcctg aggtcgtttc caaatgcttt aatggcgaga
    19381 gtggttatcc aggttgaaag gtcagtgtct acagaccatc cgccatcctc cctctgttgc
    19441 ctgcgaagaa attcaagacc tttttctatt attgaattat ctgatttccc tgctgctttc
    19501 aggcacatca caacaaatcc tgtaaggggt attgcttcaa gaaatcctcc gctttcggga
    19561 acaagtttct caagtttttt aatagcaggc tcaatgagta atgtccggat tttaccatat
    19621 aagccataag ttctcttgcc tgccctatga aggtatattc caacacctat aagtgcaggc
    19681 agagcatagc ttacaactcc cagatttaaa aacctgtaga atctttgtgg taaaattgcc
    19741 agttcaaatg gcaaccgggg tatattattt atacatgact caggtataac tccacagata
    19801 gtaagcatcg aaagtattgg gactgaaaat gtgtaatctt tcccataaaa cttcaaaata
    19861 gtgtttgtaa ttgtgtgggg attaaggtct attccattac ccgacaacca tctttccatc
    19921 ttctggagaa gtgaactaca actaccttca gtctagcaat aactaattgc actgtagcaa
    19981 agaagagttg tgctcacatt acttaaagaa ttcgttgtgt ctccaaagcc tccgtcagag
    20041 tttatatttt tgcataacca gtcgaagcct gctttgatcc tctccttatc aactggggct
    20101 ccatttacct tcagcgccac tattgcaact gccgtcgaca atgcactggt tgagagatac
    20161 cccttccaga atccgcctga gttaacctca gacttcagcc tctggcataa gtcataatat
    20221 ttccgcagta tgatttcttt ttccataatt atcatgccag tgaaccaaga gcaagaagtc
    20281 cgtaaaaagt atattcaatg tcagtctggc gatcagcaac gcttgcacaa aaacccccat
    20341 taatataatg actatcaatg aaaccaaggc attcaggttt aatcaataga agatcaaatc
    20401 cagtataact gagagcataa agtgatactg ccgttgaaag taaatcagga agagctatgg
    20461 ttttaacagc tctgaagcct ccctgattgt cataaaaatc cagaagtttc tgcctgaggt
    20521 gttcagccgg tttcttaaat gtactgtata aaataagtga tgcggcaata acagttacag
    20581 gcaggtcatc tgattgattt aaccggctta atagtttata aatttggcgc aaaacaatat
    20641 agtctctcgt ataaaggctg gcaagcagat ttacaaatgc ataatattca tttttttctt
    20701 tatgtggcat gaccacgtca cggcggatct gccttcttat tttgtttttt atttgccggt
    20761 tatctgataa ttcggtcaaa agtatcgcgg cacattgcag atgaacaccc tctggcattg
    20821 agtaactaat ttcatcttca gcatattttc ctgttctttg aagattttcc ttaagaccaa
    20881 gaacatctgc gagaaaaaaa ccgaataatg tataatacat gtcggggttg cctgctctgt
    20941 caataaatcc tccggaagga tgctgcattt tgagaacaaa atttctgaca tcgtttatta
    21001 cttcatcttt cagaagctcc agtgattttt taagaactgc ctttatgctt cttccctcag
    21061 atatctttgg atctgggaaa aagtgtgttt taaaggtatt ttccaaaaat ttttccaacg
    21121 atatcatgca aggcaatttt taaagtaatg ttattcagct cagccaggca ttcatatata
    21181 agatttatgt acattgatat cagactctca gtttcttcct taatatttga attaactata
    21241 agctccctta gtaaacaaaa atcattgtta cttacagcat tatggagagc ctccctgagg
    21301 ttgaggtcct tactttctgc gagaatagaa atcagaatat ttggattttt taaagaagat
    21361 gttgatgaca cctctttcag atcttcaaga tcatcattaa gttgataagc aatgccaaac
    21421 aggtagctaa aacgacttaa aatttttaat accctttcgt cagcaccccc ggcaatagca
    21481 cctgtaagta aggcaaccct gattgcctct ccggttttat ttttatatat ttccagtagc
    21541 tcatttacag gtaaaagaat attattcttt cttgcaagaa gttcattccc ctgtcctttg
    21601 gccatagcac tgtgtccttc ggagattatt ttcagacact caactttatc actggcatcc
    21661 atgtcacatt ccgagatcag tctgtaacct tcaccaataa gaaaatctcc tgtgttaatt
    21721 gctattggca ctccatactg agcatgaatt gtcttctttc cataacgcaa actatcttcg
    21781 tcttcaatat catcgtgaac aagggatgcc ttatgaaaac actcgattga catagccagg
    21841 tttttcaata acttttcatc tgtattattt ttgaatgctt tatagcttaa tattgccagg
    21901 agcggacgca tccttttgcc tcctgaaagc attaaagttt ttacaatttc atcgtttgtg
    21961 gaatctgaaa gcttaagtag tttatttatt tgttctgtgt taaatataga tatagtccct
    22021 tttttaagac tgtgcaggtt aagaagactg aactggttgt cattagcgta ataatggagc
    22081 tcttctctga gccaatcctc atcagcaata gtgtccgtgc aaccgttaaa aaccagagga
    22141 acaccaatag ccggtacaaa atatgagttt atggttttaa atattttctg gagagattcc
    22201 atacatgcca ctccgactat tgcttctatt ttcccgctct cgattaattt tgatgctatt
    22261 gttgtgcctt cggcaacaat ggtaagatag cccagattct cagcttcctg caaatattca
    22321 gggatattgc agttgccaca tcccccacat aataatccga attcatcttc tcctgccaca
    22381 cattttttgc tgtttttaag gcattgtggc aaaagcaata atcttctgtt atatggtata
    22441 gaagccacaa ccggcctcca tagagcattg ccacagcata ccattacaaa tgccttgtac
    22501 ctttcgtccc acccgtattt attaagtacc tcacttgcaa taacttcaag tttgtcaata
    22561 gtaactggtg gcagtaaatt ttgctccgat gtgaacaggg tcgcttttat ccttatctca
    22621 tcgcgaacct gcttctcaac aggaacaacc aatggcaata ttttttcaac agtcttttcc
    22681 atttcaatta accatatgct cctaaaccaa aaaacgctgt tttctttgca atacgataca
    22741 atagtttttt ctcagaatta atggtgatga actatggctt ggtattgatg gtgctttcag
    22801 caggctggta agataatttt cacggtttga tgtattttta gccttatgat tctccgaaaa
    22861 atgaaccagc caggcggggt cctccatcaa tatacaaccg tttgtcttct ctctgagctg
    22921 ctctttgaat tcccttaaga ataaagaatt ttcatacaag ctccctggtt caccgtattg
    22981 cagattttca caacttaact gaattaccgg acagggttca atatctcctg atggattgat
    23041 atgatggctc agtccggcaa tagcagggca aaaaggaatc cctttttcgt tccagtaaga
    23101 atcaatcaga attaacggga gccgggtacg ggcatcaaca ataaagtttc tcagctttgt
    23161 tatttcactg gtattcagag ccagttcata atgaggatca ctacctgaag gcctgtaaat
    23221 ataataccag aggtatatta cccctgaatc atgaagcata ttgacaaatt catcagaaac
    23281 agccatttca aaattcgatt tgcacacact gattgcaaca ccggttatga gtccttcaga
    23341 aacagatgtt tcaattgcct caacggtgcg gttgtagatc cggtgcccgc ctcttcttat
    23401 gtcagcaaca tattcatctc cttcaaggct tataaggggt gttacattac ctgctctacg
    23461 caaacgttga gcagttgact ttgtcaaaag agtaccgttt gtaaaaagtt gaaaataaca
    23521 atcaggatgt ttttcgaata tatcaaacaa tccatcgtat aaaagcggtt ctcctccaag
    23581 aattccgaaa aaatatgaac catatttctt gcctgcccta attacagcat caattttttc
    23641 tggctcaata ttaattttag ggccgtttac ctccacccag catccctggc aatgaagatt
    23701 gcaattattt gtgacagaga taaactgaaa agcaggaaac atactaccat ttttaattct
    23761 cttgcggaac ctgataaacc ccaatatgcc tttaacaacc atattcatca ggaatatcct
    23821 cagacagcgc ttatccgttt taataagatt tttaagtatt gacatgttta tgcctggtct
    23881 tctttatttt tcaaaaggaa tttttctctt tcttcaattg cctttctgga agcttcttcc
    23941 ctgatgatag aattcatctt caattgtctc ttgcgaagag ccaacaccag atcatctccc
    24001 aggaatgttt taacatctgc ctcccgtcta ataagttctt cattttcgtc aaaatagtct
    24061 gcaccaccaa tggcgcctcc atttttgtat ttcgggaaaa taattgctgt tgaggggcat
    24121 atccgtgcac aggctgcaca ctgattctta cagccttgag ggaagattac tttaaccttc
    24181 tcagatttct tctcatatac actgaaaagg cagaattcag cacactgcc
//
LOCUS       DAOSNB010000007        23744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2152_length_23744_cov_4.211406, whole genome shotgun sequence.
ACCESSION   DAOSNB010000007 DAOSNB010000000
VERSION     DAOSNB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23744
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2152_length_23744_cov_4.211406"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLG42_00795"
     CDS             <1..1064
                     /locus_tag="PLG42_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29775.1"
                     /translation="MGFYYTSEKKFYFKDSVKIVNPNYTVIADTMVYNTSTETAYFTG
                     PTTLEGDSIYMYCEKGWYDTKNDLSTLWNSPVINNRQHIITGDSLFYDRRTGYGEAFR
                     KISITDTSSNIIVAGNYAWYYREPEKFMVTDSALFIQVSEEDSLFLHSDTISAVTLKK
                     FPSDTSSFRLIRAYYRCKIFSKDFQAKCDSLTYSFQDSVIRLYRDPILWSEENQMVAD
                     SMAIFTRNRKTDRMELYNSVFVASQVDSLRFNQMKGRKLTGYFMNNKLYRILIEGNGE
                     TVYYLVDNNELIGINHARSASIEILIDDGKVKEIIELQNPEGTLDPPDTNSPGSLRLK
                     GFVWYDYLRPKKKSDIFIK"
     gene            complement(1160..1381)
                     /locus_tag="PLG42_00800"
     CDS             complement(1160..1381)
                     /locus_tag="PLG42_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOU29776.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGDIYESIEDIWPDYPSKEDFFFNEDEY"
     gene            complement(1473..2015)
                     /locus_tag="PLG42_00805"
     CDS             complement(1473..2015)
                     /locus_tag="PLG42_00805"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOU29777.1"
                     /translation="MKIYTRKGDDGFASLYGGRRLPKHHPRLEAYGTIDELISWIGLL
                     RSYRETVKHADFLLYIQKQLMACAAALAYDNENPSGEIMLPEEECVEKTEKEIDKLNE
                     QLPSLKSFIIPGGNIIVSNCHITRCVCRRAERNVTKLGESEKAPEIIIKFLNRLSDYF
                     FVLARFLNKDIDNENVNYNC"
     gene            complement(2071..2781)
                     /locus_tag="PLG42_00810"
     CDS             complement(2071..2781)
                     /locus_tag="PLG42_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU29778.1"
                     /translation="MNENNVIIELQDIVKNYQVGTVIVRALRSVSLTIYKNEYVAIMG
                     PSGSGKSTLMNLIGCLDTPTSGRYFLNGVDVSKMEDDRLAEIRNKEIGFVFQTFNLLP
                     RYTALENVTLPLIYAGVPKAEREKLAIETLTQVGLADRMHHKPNELSGGQRQRVAIAR
                     ALVNKPSIILADEPTGNLDSKTSIDIMNLLDDIHRKGNTIILVTHEEDIAKHAARIVR
                     LLDGQVSSDMLNEKYVKA"
     gene            complement(2855..3646)
                     /locus_tag="PLG42_00815"
     CDS             complement(2855..3646)
                     /locus_tag="PLG42_00815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU29779.1"
                     /translation="MNDFHIVINNLKYKVFSRHKRGHGIHSPFVFNLITHVFRNKIDP
                     TVVSTIENIRKEMLADPSFVKVTDYGSGSKHFRDGMRKVSSIAKYSSVPKKYGELLHK
                     FAAEYGGTDIIEMGTSLGISALYLATGARGTMVHTIEGCPVVSEIAKRNFEKAGCRNI
                     RVYTGKFDEVLPELKEKNIRPGLVFIDGDHRKDALLKYFETICSMCNDKSVVIIDDIH
                     RSVEMAEGWAEIKKMKNVSVTIDICRMGIVFLRKGISKFDYVIRY"
     gene            3732..5837
                     /gene="recG"
                     /locus_tag="PLG42_00820"
     CDS             3732..5837
                     /gene="recG"
                     /locus_tag="PLG42_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOU29780.1"
                     /translation="MQNFLDTDIKYLPGVGPRRAEQLNKELNIKCYRDLLYYFPYRYV
                     DRTKFYTVAELDPDLPYVQIRGVIKGYFTEGKGNNKRLIAEFSDETGVIRLIWFRGTR
                     WIPETYPAGKEYIIFGKPGVFNSMINIIHPEIELAEKREKRLVSGLEALYSTTEELKS
                     SFITSKFISKLVGNLLGMINFRLPETLPDYIITNNNLMSLHDAIHKIHFPSNTKEMER
                     ARYRFKFEELFYIQLNLLRYKVFRKTHTPGFIFSRVGEYFNKFYFNNLSFELTDAQKR
                     VMKEIRRDLGSGKQMNRLLQGDVGSGKTLVALMSMLIAADNGYQSCIMAPTEILAYQH
                     FRSISKFTEGIGIRVMLLTGSTKKTERKHIEKVLLSDEPAILIGTHALIEENVRFKRL
                     GLAIIDEQHRFGVAQRAKLWEKSQNPPHVLVMTATPIPRTLAMTLYGDLDVSVINELP
                     PGRKPVKTMHFFESERNKVFAFMRKQISEGRQVYIVYPLITESEKLDYKNLDEGLDIL
                     TQAFPPPQYCISVLHGRMTAANKEISMKLFREGKAHIMVATTVIEVGVDVPNATVMVI
                     ESAERFGLSQLHQLRGRVGRGAEQSYCILMSSDKLSKEASERIETMVRTSDGFEIAEA
                     DLRLRGPGDIEGTQQSGFPFELRISDLARDGQIIEYVRRIAESILEKDPLLEDDKNLI
                     LKQELNRLFSRNYSWSNIS"
     gene            5911..6327
                     /locus_tag="PLG42_00825"
     CDS             5911..6327
                     /locus_tag="PLG42_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOU29781.1"
                     /translation="MKSENQITHEGVILSSGPDSVTVMISQNVSCAGCQASGVCNLND
                     RNNKILKIDGNYNLKPGSKVIVTVNLSHGYLAVFLGYVMPFVILIASLLVTISMSLGE
                     LAAGLISLGITSFYYILLFLFRKHVSKKFSFSLKTL"
     gene            6332..7198
                     /locus_tag="PLG42_00830"
     CDS             6332..7198
                     /locus_tag="PLG42_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOU29782.1"
                     /translation="MSHTILLTVIVLGSVGLAAAVILYFVSQKFKVYEDPRIDIIQEA
                     LPGANCGGCGFTGCRNFAEALVKADTLDGLNCPVGGATVMADVAKILGKEAVVSEPLV
                     AVVRCQGSYEHRPRTSAYDGVKECRIAHLLYGGETDCHYGCLGYGDCVEACQFGAMYM
                     DEKTGLPVVIDEKCVACGACVKACPRNIIELRKKFKKDRKIYVSCVNCDRGGPARKAC
                     KVACIACNKCFNVCKFGAITIQNNLAYIDSFKCTLCRKCVTECPTSAIVELNFPPRKA
                     EEEAVKETLTQK"
     gene            7217..8551
                     /gene="rsxC"
                     /locus_tag="PLG42_00835"
     CDS             7217..8551
                     /gene="rsxC"
                     /locus_tag="PLG42_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOU29783.1"
                     /translation="MLKTFPRGGVHPPENKLTAGNPIEILPPPETVVIPVTQHLGKPA
                     VATVNKGDKVLVGQLIARAEGFVSANIHSSVSGTVSRIDTVTDISGYKQTCIIIDVSG
                     DEWLSDIDRSPDIITEIKHSPEEIIQKCLDCGIVGLGGATFPTHVKLKVPAGKKCNLL
                     IINGVECEPWLTSDHRLMLEKGDEILTGARIMMRALGVTKAIIGIENNKPDAIAHLSR
                     LLNGANDISVQGLKVKYPQGGEKQLIKALTGREVPSGGLPIDVGVVVQNAGTAYAVYE
                     AVQKNKPLFERVVTVTGKSLSKPGNFLVRVGTPVAKLIEAAGGLPENTGKIISGGPMM
                     GKALANLQTPVVKGTSGIVVFPAEESQRPQVGPCIRCSKCVSACPMGLEPYLLMSLAE
                     KGLFERMKAEKVTDCIECGSCSYICPAHRPLLDYIRLGKTTLFKMMREKSVK"
     gene            8564..9565
                     /locus_tag="PLG42_00840"
     CDS             8564..9565
                     /locus_tag="PLG42_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOU29784.1"
                     /translation="MSSLLTVSQSPHIHTGDDTKKLMRGVIIALIPAFIASVYYFGWG
                     ALIVTATSVLSCIFFEYVIQKYLIRGPVTITDGSAIVTGLLLAFNLPSNLPVHIIIIG
                     ALVAIGIGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTTWPVPSGFKTGYADAVTGA
                     TPLGVLKEGIRNGEQVSQLMNQIPDHMQMFYGHMGGSMGEVAALALLIGFVYMLVKKI
                     ITWHIPVAVTGSIALFTGILWLINPEKNADPLFHLLTGGVMLGAIFMATDYVTSPMVP
                     KAMIIYGIGIGVITVIIRVYGAYPEGVSFAILIMNAFVPLMNAWIKPKRFGEEVKNG"
     gene            9558..10133
                     /locus_tag="PLG42_00845"
     CDS             9558..10133
                     /locus_tag="PLG42_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOU29785.1"
                     /translation="MAKIESSLKNMIMSLTFIALGASAALGFVYEITKRPIELTKLNK
                     KLEAIKAVVPSFDNNPDQEMFKLPTGEGDSLEVFPAKINNEIIGYAVRTSTQKGFSGY
                     IGIMVGFSPDGTIINTNVLEQKETPGLGTKMTEPSFKDQFNGKNPDNFKLRVKKDGGE
                     VDAITAATITSRAFCDALERAYNTLKKGGLI"
     gene            10130..10711
                     /locus_tag="PLG42_00850"
     CDS             10130..10711
                     /locus_tag="PLG42_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOU29786.1"
                     /translation="MSLAKQFTKGFFRENPILVLLIGMCPTLATTTSAINGLGMGIAT
                     TFVLTSSNVVISLLAKYIPDKVRIPSFIVVIASFVTIVDLVMQAYVPGLYETLGIFIP
                     LIVVNCIVLARAEAFASKNKLLPSFLDGLGMGLGFTMALGILGTIREILGSGSVFGYK
                     FIKGDGILVFILAPGAFIALGYLIAFINKVRKA"
     gene            10724..11299
                     /locus_tag="PLG42_00855"
     CDS             10724..11299
                     /locus_tag="PLG42_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfA"
                     /protein_id="HOU29787.1"
                     /translation="MEYIIIIISAIFVNNIVLAQFLGVCPFLGVSNKVSTSFGMAGAV
                     TFVMVLATMVTYLIYTYILVKLGIAFMQTITYILVIAALVQMVEIILKKISQPLYQAL
                     GIFLPLITTNCAVLGVTLLVIKKHYNLLEGVVYSAATAIGFGLALVILAGIREQLDLL
                     NVPKGMKGAPISLITAGILALAFMGFSGIVR"
     gene            complement(11312..12187)
                     /gene="zupT"
                     /locus_tag="PLG42_00860"
     CDS             complement(11312..12187)
                     /gene="zupT"
                     /locus_tag="PLG42_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOU29788.1"
                     /translation="MDSAVIIRALIMTFIAGMATGAGGLVAFFARRTKPSFLSFSLGF
                     SAGVMIYISFTELLPESRLNFSGVYSENVSSWLSFIFLVIGFILTALIDKFIPAVDNP
                     HEARDVEQIAEIEATRPLVNPEVGQKRRHRWGHYSSIRDTRLMRVGIFTAFAIFVHNI
                     PEGIATFISGISDFTIGVSIALAIAIHNIPEGIAVSVPVYMATGSRKKALIWSVMSGF
                     SEPIGALLGICLISVIKGSALLGYIYAIVSGIMIYISFDELLPSAHRYGKHHTAIYGL
                     LSGMIIIGLSLLLLS"
     gene            12297..12878
                     /locus_tag="PLG42_00865"
     CDS             12297..12878
                     /locus_tag="PLG42_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOU29789.1"
                     /translation="MHTSVHISEYYRILGLTRGCTVDDIKKSYRKIAREYHPDLNQDP
                     GAKDIFISATEAYEFLMANHSRIASAESEYERVMEEWKKFRSEQARRRASAYARTSYS
                     KFRNTDFYKSTRIFDGTTIIASIIISLMVLIIAIYGYFYRLHHPIPGLKDPSVFILII
                     FILLGMTLFIKSYIHLKAYIESSKRKSDVQKNS"
     gene            12859..13341
                     /locus_tag="PLG42_00870"
     CDS             12859..13341
                     /locus_tag="PLG42_00870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOU29790.1"
                     /translation="MFRKIVNPFDRKLNMCFGCGPENPAGLKLHFTECKGELQAKWLP
                     SEHYQGYPGVLHGGITAALLDETAAWFVYTVIGTAGVTYTMNVRYHHPVYINKGEVKI
                     TAKLINREDKNALILCQLTDSSEKLCAEAELNYFLYPVKIAKQKFKYPGKEAFYSNDG
                     "
     gene            13399..14319
                     /locus_tag="PLG42_00875"
     CDS             13399..14319
                     /locus_tag="PLG42_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU29791.1"
                     /translation="MKFLIVFSTAMAMLVSFSGKAEKPVVSAEMQEQTPLFSFGIITD
                     VQYCDCEPAGSRFYRNSFQKLEEAFNTFKAENPAFIINLGDLIEKDYSSFLPVLKIME
                     NSGFKIYYTTGNHDFTVESKYKKRIHSLLGLKEEYYSFSYDKFRFIVLNGNEISTYGS
                     GGRATIRQAEEMISKLKAEGEPNYHDWNGGISEKQISWLKSRLNESSAKGEKVFIVCH
                     FPVWPENEHNLFNYREIVDLLGNYNNIIAWFSGHNHAGNYGNSNMVHHYTFKGMVETE
                     SSNSYAIVEVYKNKIWIKGYGREKNMILAY"
     gene            complement(14356..14736)
                     /locus_tag="PLG42_00880"
     CDS             complement(14356..14736)
                     /locus_tag="PLG42_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU29792.1"
                     /translation="MGELRIEKGKNTPEIILDPEGVIQITGRSIHENAEEFYQPVEEW
                     INEYIKNPADITVVNIHMEYFNSASARVFIKLFQKIIYVTVKDKKYIFNWYYDEGDED
                     ILERGEYFSAVLSVPFNFIELKGK"
     gene            complement(14826..15842)
                     /gene="tsaD"
                     /locus_tag="PLG42_00885"
     CDS             complement(14826..15842)
                     /gene="tsaD"
                     /locus_tag="PLG42_00885"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOU29793.1"
                     /translation="MENITILGIESSCDDTSAAVIRDGYLLSNLISSQELHRVYGGVV
                     PELASRAHQINIVPVVDRALKEAGVEIQDISAVAFTRGPGLIGSLLVGVSFAKGLALA
                     RDIPLIEVNHLHAHVLAHFIKRKDKENRIPSFPFLCLLVSGGHTQLIVVRDYYTMGLI
                     GNTIDDAAGEAFDKCGKLMGLPYPAGPVIDKLAASGNKNAFRFAKPSVPGLDFSFSGL
                     KTSFLYFLRDRLNENPDFIKENLPDLCASLQDAIISVLITKLVKAAEITGIEEIGIAG
                     GVSANSAFRQALKEEGEKRGWNVYIPEFIFTTDNAAMIAITGYYRYLKGEFASHDVIP
                     LARM"
     gene            15901..20415
                     /locus_tag="PLG42_00890"
     CDS             15901..20415
                     /locus_tag="PLG42_00890"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOU29794.1"
                     /translation="MLKSIKNIARVVIITITTITVLILMLLLLLSISPVQTIISKKLS
                     EYISEELNTTISVDRIKYTYFNRLNIANLLIKDKNQDTLIYIPLLRVSIRKYEPGTGS
                     LTLGRIIATRPQVSLITDSTGVMNLTWYLDKFRKKDSEHGKGKGKIEINRIDLNNGRF
                     RLVNITKPHSKTPVDFNNLRLDSLYATVTKLNIAGDSVKMDIKDLRFRESSGFEVKNF
                     SSELSVSGRKIIFYNSLIESDSSLINAPVIALLPDSVTKFRNFESGVGLQIQLTESKI
                     ACSELRYLVPLKTLPSNIFNIKGTVTGTISELKGRNISIAFEDNSKAEINISLSGLPD
                     INNTFIFFEIKQLETFPMELEKLKIPGAKPIIIPDNLRDMGKIAFSGSFTGFITDFVT
                     YGRLYTDAGIISSDISLRPEGAKHFRIKGLLKCENIDAGRIAGNSDLFGKSTLIADIN
                     GETESFKTFAFSVNGKIDSIEINKYKYRDITLNGYFTEKTWDGTIKAEDENIRMELLG
                     MFDFSKELPEFDFTLNLKNADLYRLNIDKKDSTSAASLLLTANFRGNNIDNLDGEIKL
                     LNSSFRKYGQNLEVYDFSLKTFSRENVRSLLLNTDFFDMEINGDYNFATLGTEFKKMF
                     SEIFPSRFSTSLKNTENNTNNFRLKVNFKNINALNNFLKTGLSISENSIISGFVQPDS
                     IIHINGTIGTLGIKNNYFSNLSFDLNYNDSLFKGGISTSSLSILNLTELKNFKIGLST
                     FPDHFQTAMEWDDRSKVANRGTFKAEGEFVSKTDNGITGKKYYLKLGILPGEIYVRNN
                     KWDINPAEIIIDTNSIKISRFLISSNENYFSIEGSASHDRQDTIYIKLNNINISGLNN
                     LYEKKAGEKNTIHLAIGGILSGTISLTDIYRNFMFESNIRINNFALLESNYGTVTISS
                     AWNKSSKVADIEFNNNIDGRKTFDITGYYDPENRLIDLTARADRLPVEILNPLLKTFA
                     SDISGMASGKVRLLGEPGNITLTGSLYADNSAIKIDYLQTKYRFSDSIRFDKNAIRFN
                     NIVFRDERGNTGSLNGSVYHKNFKDYSVDLTVRTNGCMVLNTREKDNEIFYGTAFASG
                     VTTIKTIGQVLRFDISARTGRNTRFFIPLNTGMSVAENTFVSFISSESEKQKEVSKSV
                     VYQQPQSPKTALEMAFDLEVTPDAEIQLIMDPKAGDIMKGSGSGNLNLSLDRKGVFKI
                     FGDYTIESGDYLFTLGNIINKPFTVQSGGKITFNGELDRADIDIKAVYRTKASLYEIM
                     PGMLSDEKLKERIPVECLLILTGKLFNPVVGFDINLPTADEETRAYLRSMIKSEEEMS
                     KQFLFLLVMNSFYADPSAGIQTRKADIGSATVGVTTMEMLSNQLSNWLSQISKDFDIG
                     VVYRPGSSALPNSQELQVALSTQLLNDRVTINGNFDVAGNQAAGRIGTSSTNTITGAF
                     DIEYKISERLRFKFFNRSNDNLYIDNGVQYTQGIGIFYKQDFDRFRDLFVKKEKSPGK
                     KEEEVKVVDK"
     gene            20545..21231
                     /locus_tag="PLG42_00895"
     CDS             20545..21231
                     /locus_tag="PLG42_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOU29795.1"
                     /translation="MGYLLLLPQDTGLQGAGEMKMTLLDLAIKGGWVMIPIILLSFIA
                     AYIFIERYYVIKRASKEDLNFMNRIKDYIHDGKIDAAVALCRSTDSPSARMVEKGISR
                     LGRPLHDINTAIENVGKLEISKLEKGFPTLATIAGAEPMLGFLGTVIGMVKSFYAMSQ
                     AGSNVEISTMSSGIYTALITTVAGLAIGILCFFAYNFLVERVEKVVFNLEATLTEFMD
                     ILNEPVKTGK"
     gene            21233..21625
                     /locus_tag="PLG42_00900"
     CDS             21233..21625
                     /locus_tag="PLG42_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOU29796.1"
                     /translation="MALTTRNKINISFSSVGMTDVVFNLLIFFMLTSTLIHPTALKLN
                     LPKGSVQVSAKPQTTVSITKDLKLYVENQQISFDDLEAVLKQKLSANPDIYIALHTDE
                     SVPIGVVTKVLNVAQKNNYKLILATTPK"
     gene            21606..22505
                     /locus_tag="PLG42_00905"
     CDS             21606..22505
                     /locus_tag="PLG42_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOU29797.1"
                     /translation="MQLHQNSSVRRIPSEKGKGLAGTILFHAGLFILFLVIGFSVRLP
                     DKPESGILVNFGFDDTGSGLIEPSGLPSQPEATPLPVQAQEETTVKDEALLTQDFDKE
                     VPEVKKADPEAEKKKQEQIEAEKKRQADLEAERLKREKEEEERRRLEEEQKRVNEILN
                     KTKNALAGAKNTGTDSRGEGVAGGQGNQGVPEGSPDSKIRGEGSGLGDRGISYSLEGR
                     GFQSLPPPKYDYQGEGRVVVEVSVDRNGKVIQAIPGAKGSTTLDEYLLNAAKEAALKA
                     VFEPKPDAPVIQKGTITYNFVLK"
     gene            complement(22542..22922)
                     /locus_tag="PLG42_00910"
     CDS             complement(22542..22922)
                     /locus_tag="PLG42_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU29798.1"
                     /translation="MDKGKIIFVVDDDPFVTSLIKKRFTSEGYNVETFNYGEDCIKAM
                     SQKPDLVILDYYFTKPGQQVMNGMEVFDRIKEIYPDVIVIMLSGQEKGEIVLELARKG
                     IDDYVIKDNNLLDNLSVSIKDLLG"
     gene            complement(22988..>23744)
                     /gene="glyA"
                     /locus_tag="PLG42_00915"
     CDS             complement(22988..>23744)
                     /gene="glyA"
                     /locus_tag="PLG42_00915"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018920153.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU29799.1"
                     /translation="MRKIADDVGAVLMVDMAHPAGLIAAGLLNNPLKYAHIVTSTTHK
                     TLRGPRGGIILMGKDYENPWGLTTPKGEIRMMSSVINSAVFPGVQGGPLEHIIAAKAV
                     AFGEALEPQFKKYQMQVKRNAAVMAEAFIKKGYKIVSGGTDNHLMLIDLRTKFPDLSG
                     KKAENTLVRAKITVNKNMVPFDTRSPFLTSGLRVGTPAITTRGLKEEHMPLIVELIDE
                     VLTNIDNEEVINRVGEKVKEMMKPFPLFNEDAF"
BASE COUNT         7545 a         4638 c         4739 g         6822 t
ORIGIN      
        1 tcatgggatt ttactacaca tcagaaaaga aattttactt caaggatagt gtaaagattg
       61 taaacccaaa ttatactgtt attgccgaca ccatggtata caacacatct actgaaactg
      121 cctatttcac aggacctaca acacttgaag gtgacagtat atatatgtat tgcgaaaaag
      181 gatggtacga cacaaaaaac gatctgtcta ctttatggaa ttcacctgta attaacaaca
      241 ggcagcatat tattacaggt gattcacttt tctacgaccg acggacaggc tatggtgaag
      301 cattcagaaa aatcagcatt acagatacat ccagcaacat tatcgtagcg ggtaattatg
      361 catggtacta cagggaaccg gaaaaattca tggtaaccga cagtgccctt tttatccagg
      421 tatcagaaga agattcattg tttctgcatt cagacacaat aagtgcagtt acattaaaaa
      481 aattcccttc cgacacttcc tcattcagac ttatacgtgc ctactataga tgcaaaatct
      541 tcagcaaaga ttttcaggca aagtgtgatt cgctcaccta ttcttttcag gactcagtaa
      601 taaggcttta cagggatcca attttatggt cggaagagaa tcagatggtg gcagattcaa
      661 tggcaatttt caccagaaac cggaaaactg acagaatgga actttataat tctgtatttg
      721 tggcaagcca ggtagattct ctcaggttta accagatgaa gggcagaaag cttacaggat
      781 attttatgaa caacaagtta tacaggattt tgattgaggg aaatggcgag acagtatatt
      841 atcttgttga taataacgag cttataggca taaatcatgc ccgcagcgcc tcaattgaaa
      901 ttcttattga cgatggaaag gtaaaggaaa taattgagtt acaaaaccct gaaggcactc
      961 ttgatcctcc tgataccaac tcacctggaa gtctaagact gaaagggttc gtctggtacg
     1021 attatttgag gccaaaaaag aagtcggata tattcattaa ataaagctgg gcgcaaaaaa
     1081 aatttcattt aaagctctgg aagtaaaaaa aatgtatatt ttagataaaa aaaggggctg
     1141 tttttacagc cccgacagtt taatactcat cctcattaaa gaaaaagtcc tctttacttg
     1201 ggtaatcggg ccagatatct tcgatgctct cgtagatatc gccctcatcc tcaatctcct
     1261 gtaaattttc tataacttca agtggagcac ctgacctgat ggcataatca ataagctcat
     1321 ccttggtagc tggccatgga gcatcctcaa gtttcgatgc taattcaagt gtccaataca
     1381 tatccaggaa gtttttatgt tgtttaaaaa aaggttcgca aatataaaaa attttataaa
     1441 ttatccaata atcgaaaaag ttttaaaaaa ttttaacaat tgtagttaac attttcatta
     1501 tcaatatctt tattcaaaaa tcgtgccagc acaaagaagt aatccgacag ccggttcaga
     1561 aattttataa ttatttccgg agctttctca ctctcaccca gtttggtaac atttctttct
     1621 gctctacgac atacacatct ggtaatatgg cagttagata caatgatatt accgccaggt
     1681 ataataaaac ttttcaaaga agggagttgt tcattcaatt tgtcgatctc tttttctgtt
     1741 ttttccacgc attcttcctc cggtagcatt atttcacccg aaggattttc attatcgtat
     1801 gccagtgctg ctgcacatgc catcagctgc ttctgtatat aaagcagaaa atcagcatgt
     1861 tttactgttt ccctgtaact ccgcagtaag ccaatccagg aaatcagttc atcaatagtg
     1921 ccataagcct ccagcctcgg atggtgcttg ggcagtcttc tgcctccata caatgaagca
     1981 aacccgtcat cacctttacg ggtatagatt ttcatttttt agaaatactg tttgcttttt
     2041 atgcccttaa gataatataa aaaccttttc tcaggctttt acatatttct catttagcat
     2101 gtcagacgaa acctggccgt cgaggagtct tacaatacgt gcagcatgct tggcaatatc
     2161 ctcttcgtga gtaaccagaa taatggtatt gcctttacgg tgtatatcgt ccagcaggtt
     2221 cataatatct atcgaagtct tgctgtcgag attaccagtt ggctcatcag ccagaattat
     2281 ggatggtttg tttacaagtg cccttgcaat agctactctc tgcctctgac cacccgaaag
     2341 ttcattgggt ttgtgatgca tacggtcggc cagaccaacc tgggtgagtg tctcaattgc
     2401 tagcttttca cgctcagcct tgggaacacc agcataaatc aacggtaaag taacattttc
     2461 cagagcagtg tatcgtggaa ggagattaaa agtctgaaat acaaatccta tttctttatt
     2521 tcttatctct gcaagcctgt catcctccat cttactgaca tccacaccat taagaaaata
     2581 tctaccactg gtcggcgtat caaggcatcc tataaggttc atcaatgtgg atttccctga
     2641 acctgaggga cccataattg ctacatattc gtttttgtaa atagtcagtg ataccgatct
     2701 gagcgccctt acaattacag tgccaacctg gtaattctta actatgtcct gtagctcaat
     2761 aataacatta ttttcattca taaaagcctg aaatttaaag tcccgaaaat agagcaatta
     2821 atcttaaaat cgaattatta tttattaatt tatgttaata tcttatcaca taatcaaatt
     2881 ttgaaatccc ttttcttaaa aaaactattc ccatacggca aatatcaatt gttacactaa
     2941 catttttcat cttctttatt tctgcccatc cctctgccat ttccaccgac ctgtgtatat
     3001 cgtcaataat aaccacactt ttatcattac acatactgca tatagtttca aaatatttaa
     3061 gcagggcatc tttcctgtgg tctccgtcaa tgaatacaag cccgggtctg atattcttct
     3121 ctttcagttc tggaaggact tcatcaaact tcccggtata aactctgata tttctacatc
     3181 ctgccttttc aaagttcctc tttgcgattt ctgaaactac cggacaaccc tctattgtat
     3241 gaaccattgt gcctctggct ccggttgcaa gatataatgc actgataccc agagacgttc
     3301 ccatctcaat tatgtccgta cctccatatt ccgctgcaaa tttgtgcaaa agctcaccat
     3361 attttttagg cactgaagaa tactttgcta tcgacgacac ctttctcatt ccatccctga
     3421 aatgcttcga tcctgaacca taatcagtta ctttaacaaa tgaaggatct gcaagcattt
     3481 cttttcttat gttttctata gtagagacaa cagtcgggtc gattttattc ctgaaaacat
     3541 gagtaatcaa gttgaaaaca aaaggactgt gtatgccatg acctctttta tgccgggaga
     3601 aaactttgta tttcagatta ttaattacaa tatgaaaatc gttcattttt gataccgggt
     3661 atgattatgt agggtttgca aaaatactta ttttcggaaa ataaaattta aagcgactca
     3721 taacaaattg tatgcagaat tttctggata ctgatatcaa atacctgccg ggtgttggtc
     3781 caagaagggc tgaacagctg aataaggagc ttaatatcaa atgctacaga gatctgcttt
     3841 attattttcc ttacagatat gttgacagaa ccaaattcta tactgttgca gaacttgatc
     3901 ctgacctgcc ttatgttcag atacgcggtg taataaaagg atacttcaca gaaggtaaag
     3961 gtaacaacaa aaggcttata gccgaatttt cagatgaaac gggagttatc aggttaattt
     4021 ggttcagggg aaccagatgg atacctgaaa cctatcctgc aggaaaagag tacataatct
     4081 tcggtaaacc aggggttttc aatagtatga taaatattat tcaccccgaa attgaacttg
     4141 ccgaaaaaag ggaaaaaaga cttgtgtcgg gtcttgaagc actctatagt actactgaag
     4201 agctcaaaag cagttttatc acatcgaaat ttatcagcaa gcttgttggt aacctgcttg
     4261 gcatgataaa cttcaggctg cccgagacac tccctgatta cattataacc aataacaatc
     4321 ttatgagttt gcatgatgca attcataaga tccatttccc ttccaataca aaggaaatgg
     4381 aaagagcccg gtaccgtttc aaatttgaag agctctttta tattcaactt aacctgttgc
     4441 gctacaaggt ttttcgaaaa acccatactc ctggatttat cttttccaga gttggtgaat
     4501 attttaacaa attctacttc aacaatttat ctttcgagct aactgatgcc cagaaaaggg
     4561 ttatgaagga aatacgcagg gatttaggct cgggaaagca gatgaacagg ttacttcagg
     4621 gtgatgttgg aagtggtaaa acacttgttg cattaatgtc gatgctgata gctgccgaca
     4681 atggatatca gtcgtgcata atggcaccca cagaaatcct tgcttatcag catttcagat
     4741 caatttcaaa gtttaccgag ggaataggta tcagggtaat gttgcttacg ggctccacaa
     4801 aaaaaaccga gaggaaacat attgaaaagg tgttgctcag tgatgaacct gcaatattga
     4861 ttggaaccca tgccctgatt gaagaaaatg taaggtttaa aaggcttggg ctggcaatta
     4921 ttgacgaaca acatcgtttt ggcgtagcac agcgggcaaa gctatgggag aaaagccaaa
     4981 atccgcccca tgtactggtg atgactgcca cacccattcc aagaacactt gccatgacac
     5041 tctatggcga cctcgatgta tcggttataa atgaattacc accgggaagg aagccggtaa
     5101 agacaatgca tttctttgaa tcagaaagaa ataaggtctt cgcttttatg aggaagcaga
     5161 taagcgaagg aaggcaggta tatattgtct atcccctgat aactgagtcg gagaaactcg
     5221 actataaaaa cctcgatgaa ggactcgata ttctcactca ggcctttcct cccccacagt
     5281 actgcataag tgtgcttcat ggcaggatga cagcagccaa caaggaaatc tcaatgaagc
     5341 tgttcaggga aggcaaagct catataatgg ttgccacaac agttatcgag gtaggtgtgg
     5401 atgtacccaa cgctactgtg atggttatag aaagcgccga gagattcgga ctttcccaac
     5461 tacatcagct aagaggaagg gttggcaggg gtgccgagca gtcgtattgt attctgatga
     5521 gctcagacaa gctgtcgaaa gaagcatctg aacgaattga gacaatggtc agaaccagcg
     5581 acgggttcga aatagctgaa gccgacctga gactcagagg tcctggtgat attgaaggca
     5641 cacagcagag tggattcccc tttgaactga gaataagcga cctggcacgc gacggacaaa
     5701 tcattgaata tgtcagaaga attgcagaat cgattctcga aaaagaccct ctgcttgaag
     5761 atgataaaaa cctgatcctg aaacaggaac ttaacaggct cttcagcagg aattattcgt
     5821 ggagtaatat cagctgataa aaaaatgata aaaagcagaa ataattttaa gcaggaaata
     5881 taaatttgcc actctgattt accggggaaa atgaaatcag aaaaccagat aactcatgaa
     5941 ggtgttattt tatcatcggg acctgattcg gttactgtaa tgatatcaca gaatgtaagt
     6001 tgtgcggggt gtcaggcttc aggagtatgt aatctgaacg atcgcaacaa taagatactt
     6061 aaaatagatg gaaattacaa tctgaagccc ggaagcaaag taattgtaac tgtcaaccta
     6121 tcacacggtt atcttgccgt tttcctgggt tatgttatgc cttttgtgat tctgattgca
     6181 tctcttttgg tgacaatctc catgtcatta ggtgaactcg ctgcaggatt aatttcgctg
     6241 ggaataactt cattttacta tatattgctc tttctttttc gtaaacatgt ttcgaagaaa
     6301 ttttcattca gtttaaaaac attataacca gatgagtcat acaattctgt tgactgttat
     6361 agttctaggt tcggtcggac tggctgcagc agttatatta tactttgtct ctcagaaatt
     6421 caaagtttat gaagatccga ggattgatat aattcaggaa gctctgcctg gtgccaactg
     6481 cggaggttgt ggttttacag gttgcaggaa ttttgcagag gcacttgtta aagctgatac
     6541 attggatggt cttaactgcc cggtaggcgg agcaacagtg atggctgatg tagcgaaaat
     6601 acttggcaaa gaggctgttg tatccgagcc tcttgtagca gtggtaagat gtcagggaag
     6661 ttatgagcac cggccacgca catctgcata tgatggtgtt aaagagtgcc ggatagccca
     6721 ccttctttat ggaggtgaaa cagattgtca ttacggttgc cttggctatg gtgattgtgt
     6781 agaggcatgc cagtttggtg caatgtacat ggacgaaaaa accgggctgc ctgtagttat
     6841 tgatgaaaaa tgcgtggcat gcggtgcttg tgtaaaagcc tgtccaagaa acatcattga
     6901 gctcaggaag aaattcaaga aagacaggaa gatttatgta tcatgcgtca actgcgacag
     6961 aggcggcccc gcgagaaaag cctgcaaagt tgcatgtatt gcatgtaaca agtgcttcaa
     7021 tgtctgcaag ttcggagcca tcaccattca aaacaacctt gcatatatcg attcattcaa
     7081 gtgcacactt tgccgtaaat gtgtgactga atgccctaca agtgctattg tagagctaaa
     7141 tttccctcca cgcaaggctg aggaagaagc agtaaaagaa acattaaccc aaaaataatt
     7201 atatacgacc cctactatgc taaaaacatt tccgcgcggc ggtgtccatc cgccagagaa
     7261 caaactgaca gcaggcaacc cgattgagat acttccgcca cctgaaacgg ttgttatccc
     7321 ggttacccag caccttggaa aacctgcagt agcaacagtt aataagggtg ataaagttct
     7381 ggttggacaa cttatcgcca gagcagaagg atttgtttcg gcaaatatac attcatcagt
     7441 atcaggtaca gtatcaagaa tagatacagt aacagacatc tcaggttaca aacagacatg
     7501 cattattatt gacgtatcgg gagatgaatg gctgagtgac atagatagaa gtcctgatat
     7561 tataactgag attaaacatt cgcctgaaga aataattcag aaatgcctcg attgcggtat
     7621 tgtaggactt ggcggtgcaa ctttccccac ccatgttaag ctgaaagtcc ctgcaggtaa
     7681 aaaatgtaat ctactcatta tcaatggtgt agaatgcgaa ccctggctta catctgacca
     7741 ccgccttatg ctcgaaaaag gagacgaaat tcttacaggg gcaagaatca tgatgagagc
     7801 acttggggtc acaaaggcaa ttatcggcat tgaaaacaac aaacctgatg caattgcaca
     7861 tttaagcagg ttgctaaatg gggctaacga tattagtgta cagggattaa aagttaaata
     7921 ccctcagggt ggtgaaaaac agctgattaa agcacttact ggaagggagg ttccttcagg
     7981 tggtttaccg attgatgttg gagtggtagt tcaaaacgct ggtactgcat acgctgtata
     8041 cgaagctgtg cagaagaata agcctttatt tgaaagggta gttaccgtga caggtaagtc
     8101 actttcaaag cctggtaatt tccttgttag ggtgggcaca ccggttgcga agctcatcga
     8161 agcagcagga ggattgcctg aaaatacggg gaagataata tctggaggtc ctatgatggg
     8221 aaaagcactg gcaaaccttc agacacctgt tgtgaaaggc acttctggta ttgttgtgtt
     8281 tcctgctgaa gaatcacaaa gacctcaggt aggtccctgc attcgctgca gtaaatgtgt
     8341 ttcggcctgc ccgatgggac ttgaacctta tcttcttatg agtctggctg aaaaaggcct
     8401 gtttgaaaga atgaaggcag aaaaggttac cgactgcatt gaatgcggtt cgtgcagcta
     8461 tatatgtccg gcccacaggc cccttctgga ctatatcagg cttgggaaaa ccactctgtt
     8521 taaaatgatg cgtgaaaaaa gtgtgaaata atcatacaac agaatgagca gtttgttaac
     8581 agtttcacaa tcaccccata tccataccgg ggatgataca aaaaagctga tgcggggagt
     8641 tattatcgcc ctgattccgg ctttcattgc ttcggtttat tatttcggct ggggagcact
     8701 aattgtcact gcaacatcag tattgtcgtg catattcttt gaatatgtaa ttcagaaata
     8761 tctgatcaga ggtccggtta ccatcactga cggctctgca atagttacag gacttttact
     8821 ggccttcaac ctgccttcaa atctacctgt acacattata ataatcggtg ctctggtggc
     8881 aattggcata ggcaagatga gttttggcgg attgggtaat aatccgttca atcccgctct
     8941 ggtaggacgt gtgttccttc tgatatcttt tccggttcag atgacaacat ggcctgtacc
     9001 atcaggtttc aaaaccggat atgctgatgc agtaacaggg gctacacctc ttggggtact
     9061 taaagaaggt atcagaaacg gcgaacaggt atcacaactt atgaaccaga tcccggatca
     9121 catgcagatg ttttatggac acatgggcgg gtcgatgggc gaagtagcag cactggcatt
     9181 gcttattggt tttgtttata tgctggtaaa aaaaataatt acatggcaca ttcctgttgc
     9241 tgttacaggc agtatagcac tattcaccgg tattctttgg ctgattaatc cggagaagaa
     9301 tgccgatccc ctgtttcatc ttcttacagg aggtgtcatg ctgggagcta tatttatggc
     9361 aacagactat gttacatcac ctatggtacc aaaagcaatg atcatttacg gtataggtat
     9421 cggggtaata actgtaataa taagggttta tggagcatat cccgaaggtg tttcatttgc
     9481 cattcttatc atgaatgctt ttgtacccct tatgaatgca tggataaaac caaaacgttt
     9541 cggagaggag gttaaaaatg gctaagatcg aatcttcgct taagaacatg attatgtcgc
     9601 ttacctttat agcacttggt gcttcagcgg cactgggttt cgtttatgaa ataacaaagc
     9661 ggccgataga attaacaaaa ctcaataaaa agctggaggc cattaaagct gtggttcctt
     9721 cctttgacaa caaccccgac caggagatgt tcaagcttcc aacaggtgaa ggcgacagcc
     9781 ttgaagtttt ccctgccaaa ataaataatg aaattatcgg gtatgcagtc agaacatcaa
     9841 cacaaaaagg tttcagtgga tacattggaa taatggtagg ttttagtcct gacggcacaa
     9901 ttataaatac aaacgtcctt gaacaaaaag aaacccctgg tcttggaaca aagatgacgg
     9961 aaccctcttt caaagaccag tttaatggaa aaaatccgga taatttcaaa cttagagtaa
    10021 agaaagacgg tggcgaagtt gatgccataa ctgctgccac aataacatca agagcgttct
    10081 gtgatgcact ggaaagagct tataatactt taaagaaagg aggtctgata tgagtctggc
    10141 taaacaattc accaagggat tcttcaggga gaaccctatc cttgtcctgc tgataggtat
    10201 gtgtcccaca cttgcaacca ccacctcagc cataaacgga ctgggaatgg gcattgcgac
    10261 aacatttgta ctaacatctt ccaatgtagt tatctcttta ctggcaaaat atattcccga
    10321 taaagtaaga attccctcat tcatagtggt tatagcttca tttgtaacca ttgttgacct
    10381 tgttatgcag gcttatgttc caggtttgta tgagactctt ggcattttca ttccgctaat
    10441 tgttgttaac tgtatcgttc ttgcaagggc tgaggctttt gccagcaaga ataagttact
    10501 tccctccttt ctcgatggac tgggtatggg acttggattt accatggcgc tagggatact
    10561 tggaacaata cgtgaaatac tcggaagcgg ttcagttttc ggatacaagt tcataaaggg
    10621 tgacggaata ctggttttca ttctagcacc cggtgcattt atagcactcg gctatcttat
    10681 tgcatttata aacaaagtca ggaaagcata ggaggaaaag attatggaat atattatcat
    10741 tatcatctct gccatctttg taaataacat cgttcttgcg cagtttcttg gtgtttgtcc
    10801 ttttctaggt gtatcaaata aagtcagcac ttcatttggt atggcaggtg ctgtgacttt
    10861 cgttatggta cttgctacca tggtaactta tctgatttac acatatattc tggtaaaact
    10921 gggtattgca tttatgcaaa ccattaccta catccttgta attgcagctc tggttcagat
    10981 ggttgagata atattaaaaa agataagtca gcctctttat caggcacttg gcatatttct
    11041 ccctctcata acaacaaact gtgctgttct gggtgttact cttcttgtta taaaaaagca
    11101 ttacaatctt ctcgaagggg ttgtttacag tgcagcaaca gctatcggct tcggtctggc
    11161 tctggtgatt cttgcaggta taagggagca gcttgacctt ctcaacgttc caaaaggaat
    11221 gaagggagcc cccatctcac ttataacagc aggaatactt gctcttgcat ttatgggttt
    11281 ttcagggata gtacgataaa acccttcaat ttcaggatag aagaagtaaa cttaagccga
    11341 taattatcat accgctcagc agtccgtaaa tcgcggtatg gtgtttgccg taccggtggg
    11401 ctgatggcag aagttcatca aaagatatat atatcattat gcctgataca atggcatata
    11461 tgtaacccag caaggcactt cctttaataa cagatatcag gcaaatgcca aggagtgcgc
    11521 caattggttc agaaaaacct gacatcactg accatatcag ggcttttttc ctggagccag
    11581 ttgccatgta cacaggtact gaaactgcaa tgccctccgg gatattatgt atagctattg
    11641 ccagcgcaat gcttactcca atggtgaaat cagaaatacc tgaaataaat gttgctatac
    11701 cttccggtat attatgaaca aaaattgcaa aagccgtaaa aatgcctacc cgcatcaacc
    11761 ttgtgtctct aatactgctg taatgtcccc atctatgcct ccttttctgc cccacttcag
    11821 gattgactaa cggccgggtg gcctcaattt ctgcaatttg ttccacatct cttgcctcat
    11881 gaggattatc aactgccgga ataaacttat caatcaaagc tgtaagaata aatccgatta
    11941 caagaaaaat gaatgagagc cagctggata cattttcact gtatacgccg gaaaaattca
    12001 gtctcgattc aggaagcagt tctgtaaaag agatataaat catcactccg gccgaaaaac
    12061 cgagagaaaa cgacagaaac gatggttttg tccgcctggc gaaaaaagca accagtccac
    12121 ccgcacctgt tgccataccc gcaatgaaag tcataattaa agccctgatt attacagcgg
    12181 aatccattgt ccctgttttt tttatttagt ttaaatttaa acaacaaatg tattgttttg
    12241 aaatctattt gcaaaaagca acctttacat aatatttcat tacttttata tgtaacgtgc
    12301 atacttcagt gcatatatcg gaatattacc ggattcttgg tcttaccagg ggttgtacgg
    12361 tagatgacat aaaaaagtca taccgtaaaa tcgcaaggga atatcatcct gatctgaatc
    12421 aggatcctgg agcaaaggat atattcattt ctgcaacaga agcttatgag ttcctgatgg
    12481 caaatcattc aaggatagct tctgcagaaa gcgaatatga aagagtgatg gaagaatgga
    12541 aaaaattcag aagcgaacag gcaaggcgaa gggcaagtgc ttatgcccgt acatcatatt
    12601 caaaattcag aaatactgat ttttacaaga gtacccgcat atttgacgga acaactatta
    12661 ttgcttcaat tatcatttct ttgatggtgc ttattattgc aatatacgga tacttctaca
    12721 gattacatca tccgataccc ggactgaaag atccatcagt ttttattctg ataattttta
    12781 ttcttcttgg catgacactt ttcattaaat catatattca ccttaaagcg tatattgaat
    12841 cgtcaaaaag aaagtctgat gttcagaaaa atagttaacc ctttcgacag gaaattgaac
    12901 atgtgttttg gctgcgggcc tgaaaatccg gcaggactaa aacttcattt tacagaatgc
    12961 aagggagaat tgcaggctaa atggttacca tcagaacatt accagggata tcccggtgtt
    13021 cttcacggag gaataacagc cgcactgctt gatgaaacag ctgcatggtt tgtatatacc
    13081 gtaattggca ctgcaggtgt aacttatact atgaatgtca gatatcacca tcctgtatat
    13141 attaataaag gtgaagtaaa aataacagca aagctaatca acagggaaga caaaaatgcc
    13201 ctcattctct gccagttaac agacagtagc gaaaaattat gtgctgaagc tgaattgaac
    13261 tattttttgt atccggtgaa aattgcaaaa cagaagttca aatatcctgg aaaagaggca
    13321 ttctatagca atgacggctg ataagatcaa aatcattaaa tatattttga atacatttat
    13381 tcaaaatttt aaataaaaat gaaattcctg attgtatttt caactgcaat ggcaatgctt
    13441 gtttcattct ccggaaaagc ggaaaaaccg gtagtatctg cagagatgca ggaacagaca
    13501 ccattattca gttttggtat cattacagat gttcaatact gtgattgtga acctgcaggt
    13561 tcgagatttt acagaaattc ctttcagaaa ctcgaagaag ctttcaatac attcaaggca
    13621 gagaatcctg cttttatcat aaatctcggg gatcttattg aaaaagacta ttcatcattt
    13681 ttacctgttc tgaagattat ggaaaatagc gggtttaaaa tctattatac aactggaaat
    13741 cacgacttta cagttgaatc aaaatataaa aagcggattc actctcttct gggattaaaa
    13801 gaagaatatt actcattttc ttacgataaa ttcagattta ttgttctgaa cgggaatgag
    13861 ataagcacct atggttcagg aggaagagcg actatcaggc aagctgaaga gatgatctcg
    13921 aaattaaaag cagaaggcga accaaattat cacgactgga acggtggaat aagtgaaaaa
    13981 cagatttcct ggttaaagtc ccgtctgaat gagtcctcag caaaaggcga gaaagtgttt
    14041 attgtatgcc actttccggt atggcccgaa aatgaacata atttatttaa ttacagagaa
    14101 atagttgatt tacttggaaa ttacaataac ataattgcct ggttcagcgg gcataatcat
    14161 gccggtaatt acggcaacag caatatggtt catcattata cctttaaagg tatggttgaa
    14221 acagagagtt ccaacagcta tgcaatagta gaagtttaca aaaacaaaat atggattaag
    14281 ggttacggaa gggagaaaaa tatgattctt gcttattaaa aatgtttcgt attgcagaca
    14341 atttgtataa tacttttatt tgcctttaag ctcgataaaa ttaaaaggca ctgagagcac
    14401 cgctgaaaaa tattctcccc tttccagaat atcctcatcg ccctcgtcat aataccagtt
    14461 aaaaatatat tttttatctt ttaccgttac gtaaattatt ttctgaaaaa gtttaatgaa
    14521 gactcttgca ctggcactgt tgaaatattc catgtgtata tttaccaccg tgatatcagc
    14581 aggattctta atatattcat tgatccactc ctctactggt tgatagaact cctcagcatt
    14641 ttcatggata gaacggccgg tgatctggat tactccttca ggatcaagaa taatttcagg
    14701 tgtattcttc cccttttcta tcctcagttc ccccataaaa aaactttgcg cgaatataaa
    14761 taaattttta atactgcttt tcaggaaaga tatacacaca ttaaaaacag gcagatgttt
    14821 gatgtttaca ttcttgccag tggaataacg tcgtgtgatg caaactctcc tttaagatat
    14881 ctgtaatatc ctgtaatagc tatcattgca gcattatctg ttgtaaaaat gaattcaggt
    14941 atataaacat tccatcctct tttttcccct tcctctttta gagcctgacg aaatgctgaa
    15001 ttggctgaaa cgccaccggc tattccgatt tcttcaattc cggtaatttc tgcagccttg
    15061 accagtttag ttattaaaac ggagattatt gcatcctgca gtgaagcaca taaatctggc
    15121 aggttttcct ttataaagtc aggattttca tttaatctat cgcgcaggaa ataaagaaag
    15181 ctggttttga ggcctgaaaa actgaaatcc agccctggca ctgatggctt cgcaaaccgg
    15241 aatgcgtttt tattacctga agctgcaagt ttatctataa ccggacctgc aggatatgga
    15301 agtcccatca gttttccgca tttgtcaaaa gcttcacctg ctgcatcatc aatagtgtta
    15361 ccgattaatc ccatcgtata ataatccctt acaactatca gctgggtatg accgcctgag
    15421 accagcagac agaggaacgg gaaagaaggt atcctgtttt ctttgtcttt tctttttata
    15481 aaatgagcca gaacatgggc atggaggtga tttacttcta tcaaaggaat atctctggca
    15541 agggcaagac cttttgcaaa cgatacgccg acaaggagtg aaccaattaa tcccggacca
    15601 cgtgtaaatg caaccgctga aatgtcctga atctcaaccc cggcttcttt taatgcacga
    15661 tcaacaacag gaactatgtt aatctgatgc gctctcgatg ccagctcggg cacaacgcca
    15721 ccatataccc tgtgcagttc ctgggaggaa attaaattcg acaacaggta cccatccctt
    15781 atcaccgctg ccgatgtatc atcgcacgac gattcgatgc caagtattgt gatgttctcc
    15841 acagcttttt ttttccgtat tttttaagtt attttgaaac tgaatttact gtaaaaatca
    15901 atgctcaaaa gtattaaaaa tatagccaga gtagtaatta taacaataac aactattact
    15961 gtcttaattt taatgctgtt acttttgcta agtatctccc cggttcagac catcatttca
    16021 aaaaagttat cagaatatat ttcagaagaa ctcaatacca caatatctgt tgacaggata
    16081 aagtacactt atttcaatag acttaacatt gcaaatcttc tcataaaaga taaaaatcag
    16141 gacactctta tttatattcc tcttttaaga gtcagcatcc gtaaatatga gcctggaact
    16201 ggatctctga ctttaggcag gataattgcc actcgccctc aggtatcatt aataacagac
    16261 tccaccggag taatgaatct gacctggtac cttgacaaat tcaggaagaa agattctgaa
    16321 cacggaaaag gcaagggtaa gattgaaata aaccgtatcg atttaaacaa tggacgtttc
    16381 aggcttgtaa acataaccaa accgcattct aagacacctg ttgatttcaa taaccttcga
    16441 cttgattctt tgtatgcaac agtcacgaag ctgaacattg caggtgattc ggtgaaaatg
    16501 gatattaaag atttaagatt cagggaatca tccggatttg aagtcaaaaa cttttcatca
    16561 gaactctctg taagtggcag aaagataata ttttataaca gcctgataga gagtgattca
    16621 tcattaatca acgcgcctgt aatagcactt ttacccgatt ctgttacaaa attcagaaat
    16681 tttgaaagtg gcgtgggtct tcagattcaa ctaacagaat caaaaattgc atgttctgag
    16741 ctaaggtatt tagttccttt gaaaacactt ccttcaaata tatttaatat taaaggtaca
    16801 gtcacaggaa ctatatcgga actgaaggga aggaatatca gcattgcttt tgaagataat
    16861 tcgaaagcag aaataaacat cagcctgtcg ggtcttcctg acattaacaa cacatttatt
    16921 tttttcgaga taaaacaact ggaaactttc cccatggagc tggaaaaact taaaattcct
    16981 ggagcaaaac caataattat tcctgataat ctgagagata tggggaaaat agcattttca
    17041 ggctctttta cagggttcat aacagatttt gtgacttacg ggaggttata cacagatgca
    17101 ggaataatat catccgatat ctcattaaga cctgaagggg caaagcactt cagaataaaa
    17161 gggcttctga aatgcgaaaa tatagatgct ggaagaattg caggcaattc cgacctattt
    17221 ggaaaatcga ctcttattgc ggatatcaac ggtgaaactg aatcgtttaa aacatttgcc
    17281 ttcagcgtaa acggaaaaat agacagcatt gaaataaaca aatacaaata cagagatata
    17341 actctgaacg gatattttac tgagaagaca tgggatggaa caataaaagc ggaggatgag
    17401 aacatacgga tggaattgct aggaatgttc gatttcagca aagagcttcc cgagttcgat
    17461 tttactctta acttaaaaaa tgccgatctc tacagactta acatcgacaa gaaagattcc
    17521 acttccgctg cttcattatt actgacagca aatttccgcg gaaataatat tgacaacctc
    17581 gatggtgaaa tcaaactact caattccagt ttccggaagt atggtcaaaa cctcgaagta
    17641 tacgattttt cccttaagac tttttcaagg gaaaatgttc gctcattgct gctgaatacc
    17701 gattttttcg acatggaaat aaacggggac tataattttg cgaccctggg aaccgaattc
    17761 aaaaagatgt tttccgagat ttttccatcg cgtttctcaa cctcactcaa aaatactgaa
    17821 aacaatacca ataacttcag actgaaagtt aatttcaaga atataaatgc tttaaataat
    17881 ttccttaaaa caggactgtc aatatctgaa aactcaataa tatcaggatt tgtgcaacct
    17941 gatagtatta ttcacataaa cgggactatt ggcacactcg gaataaaaaa caattatttc
    18001 agcaatcttt catttgatct taattacaac gactccctgt tcaaaggagg aatttcaacc
    18061 tcatcattaa gtattcttaa tttgactgaa ttgaaaaatt tcaaaatagg tctgtcaaca
    18121 ttccctgatc atttccagac cgcaatggaa tgggacgaca gaagcaaggt tgcaaacagg
    18181 ggaacattta aagctgaagg agaatttgta agcaagacag ataatggaat tactgggaaa
    18241 aagtattatt taaaactcgg aattctgccg ggcgaaattt atgtcaggaa taacaaatgg
    18301 gatatcaatc cggctgaaat aattattgat actaactcca tcaaaatttc cagatttttg
    18361 atttccagta atgagaatta tttcagcatt gagggatctg cgtcccacga caggcaggac
    18421 accatttata ttaagctcaa taacataaac ataagtggac ttaacaatct ttatgaaaag
    18481 aaagcaggtg agaaaaatac aattcatctg gcaattggcg gaattctcag tggcacaata
    18541 agtcttacag atatctacag aaatttcatg tttgaaagca atatccgcat aaacaatttt
    18601 gctttacttg aaagcaatta cggtacagtt acaatcagtt ctgcctggaa taagtcaagt
    18661 aaagtggcag atattgaatt caacaacaat attgatggta gaaagacatt tgatataaca
    18721 ggttactatg atcctgagaa cagattgatt gatcttacag cccgtgctga caggcttccg
    18781 gttgaaatac ttaatcctct ccttaaaaca tttgcatcag atatcagcgg tatggcatcg
    18841 ggtaaagttc gccttctggg tgaacctgga aatattacac ttactggctc gctttatgca
    18901 gataattcag caatcaaaat agattacctt cagacaaagt accgtttcag cgacagtata
    18961 agattcgaca aaaatgccat caggtttaat aatattgttt tcagagatga aaggggcaat
    19021 acaggttcgc tgaatggttc ggtatatcac aaaaacttca aagattattc tgttgacctt
    19081 accgtaagga ccaacggctg catggtactc aataccagag agaaagacaa tgaaatcttc
    19141 tacggtactg catttgccag cggagttaca acaataaaaa cgatagggca ggttttaagg
    19201 tttgatatct cagccagaac aggcagaaat acaaggtttt tcatccctct caatacagga
    19261 atgtcagttg ctgaaaacac attcgtatct tttatcagtt ccgaaagtga aaaacagaaa
    19321 gaggtatcaa agagtgtggt ttaccagcag ccacaatcac caaaaaccgc cctcgagatg
    19381 gccttcgacc ttgaagtgac acccgatgcc gaaatacaac ttataatgga ccccaaggca
    19441 ggtgatatta tgaaagggtc aggttcagga aatcttaacc tgagtctcga ccgcaaaggc
    19501 gtttttaaaa ttttcggtga ctatactatt gaaagtggtg attatttgtt cacacttggt
    19561 aatattatta acaagccgtt taccgttcag agtggaggca aaataacctt caatggtgaa
    19621 ctcgacaggg cagacattga tataaaggct gtttaccgca caaaagcatc actttacgaa
    19681 ataatgcctg gaatgttgtc ggatgaaaaa cttaaggaga ggataccagt agagtgcctt
    19741 ctgattctta caggcaagct cttcaatcct gttgtaggat ttgacattaa tcttccaaca
    19801 gctgatgaag agacaagggc atatcttcga agtatgatta aatcggagga agaaatgagt
    19861 aaacagttcc tcttccttct ggttatgaac agtttctatg ctgacccttc agctggcata
    19921 caaacaagaa aagctgacat cggttcagca actgtaggag ttacaacaat ggaaatgctt
    19981 tcaaaccagc taagcaactg gttgtcacag attagcaagg attttgatat aggtgtggta
    20041 taccgcccgg gctcatctgc ccttcccaac tcacaggaac ttcaagtcgc tctatcaaca
    20101 cagctactta atgacagggt aacgataaat ggaaattttg acgttgcagg taatcaggct
    20161 gctggcagaa ttggaacatc aagcacaaac actattacag gagcattcga tatcgaatac
    20221 aagataagtg aaaggctcag gtttaagttt tttaacaggt cgaatgataa tctctatatc
    20281 gacaatggag tacaatatac tcagggcata ggtatatttt acaaacagga cttcgacaga
    20341 ttcagggatt tattcgtaaa aaaggaaaaa agccccggaa aaaaagagga agaagttaag
    20401 gtagttgata aataacctct ttttgttatt aaaccggaaa taatataaaa aacattgcct
    20461 cgtttttaaa taaatttgca tccgagacaa aaatctatta acagggtaaa ttataattgt
    20521 ttataactta atctgaatta aataatggga tatctcttat tacttccaca ggacactggc
    20581 ctgcagggtg caggtgaaat gaagatgact cttcttgatc ttgccataaa aggcggatgg
    20641 gttatgattc ctattattct gctctcattt attgcagcgt acattttcat tgaaaggtat
    20701 tatgttataa aaagggcaag caaagaagac ctcaacttca tgaacaggat aaaggattac
    20761 atacatgacg gaaaaattga tgcagccgtt gcattatgca gatcgactga ttcgccatcg
    20821 gcgaggatgg ttgaaaaagg cataagcaga ctgggaaggc cattgcacga tatcaatact
    20881 gctattgaga atgtcggaaa acttgagatt tcaaagcttg agaaaggttt tccgactctt
    20941 gcaactattg caggtgcaga accaatgctg ggcttccttg gtacagtaat cggtatggtt
    21001 aaatcatttt atgcaatgtc gcaggcaggt agcaacgttg aaatatcaac catgtcgagc
    21061 ggtatttata cagctcttat aaccacagta gcaggtctgg caataggtat tctttgcttc
    21121 tttgcttaca atttcctcgt tgaaagagtt gaaaaagttg tattcaacct tgaggctaca
    21181 ctcactgaat tcatggatat tctcaacgaa cctgtaaaaa ccggtaagta agatggctct
    21241 aacgacaaga aataagataa atataagttt cagttctgtc ggaatgacag atgttgtatt
    21301 taacttactt attttcttca tgttaacatc aacactgatt catccaacgg cattgaaact
    21361 gaatctgccc aaaggttcgg tgcaggtatc tgcaaagcct cagacaactg tttcaataac
    21421 aaaagacctg aaactttatg ttgagaacca gcagataagt tttgatgatc ttgaggcggt
    21481 tctgaagcag aagcttagtg ctaaccctga tatttatatt gctttgcata ctgatgaaag
    21541 tgtgccgatc ggggttgtta caaaagttct aaatgtagct cagaaaaaca attacaaatt
    21601 aatccttgca actacaccaa aatagcagtg ttcgcaggat accttcagag aaagggaagg
    21661 gtctggcagg aacaattctg tttcatgcag gattgtttat actctttctt gtaattggat
    21721 tttcggtacg tttacctgac aaacctgagt cgggtatact ggttaatttc ggttttgatg
    21781 atactggctc tggtctgata gaaccttcgg gtcttccctc tcagcctgaa gctacccccc
    21841 tacctgtaca ggcacaggaa gagacaacag taaaagatga agcacttctt acccaggatt
    21901 ttgataaaga ggtgcctgaa gttaaaaaag ctgaccctga agctgaaaag aaaaaacagg
    21961 agcagattga ggcagaaaaa aagcgacagg ctgacctgga ggctgaacgt ctgaaaaggg
    22021 agaaggaaga agaagaaaga agaaggcttg aagaagaaca gaaaagagtt aatgagatac
    22081 ttaacaaaac aaagaatgct cttgcagggg caaaaaatac cggaacggat tcaagaggtg
    22141 aaggcgtggc aggcggccag ggtaatcagg gcgttcctga aggctcacct gattcaaaaa
    22201 taagaggtga aggatcaggg cttggcgaca ggggcatatc ctatagcctt gaaggcaggg
    22261 gatttcaatc attgccacca ccaaaatatg attaccaggg agaaggaaga gtcgttgttg
    22321 aagtaagcgt cgatagaaac ggtaaagtaa tccaggcaat tcccggtgca aaaggttcta
    22381 caacactcga tgaatatctt ctgaatgcag ctaaagaagc agcactaaaa gctgttttcg
    22441 aacccaaacc agatgctccg gtaatacaaa aaggaacaat tacatataat ttcgttctga
    22501 aataagaaga ctggaagata agatttcaaa atcgaataat cctatccaag aagatctttt
    22561 attgaaaccg agagattatc gagaagatta ttgtctttga tcacataatc atctattcct
    22621 tttcttgcca gctctagtac aatttcaccc ttctcctgtc ccgacagcat aatcacaata
    22681 acatccgggt atatctcttt tatcctgtca aaaacttcca taccattcat aacttgctgg
    22741 cccggttttg taaaataata atcaagtatc acaaggtcag gcttctggct cattgctttt
    22801 atgcaatcct ctccgtaatt aaaagtctca acattataac cttcagaagt gaagcgtttt
    22861 ttaatgaggc ttgttacgaa agggtcatca tcaaccacaa atattatttt acccttatcc
    22921 atggtataac tttaacatgt aaatttaaca ttaaaaatta aaaaagagca tttaaccggt
    22981 tattaatcta aaatgcatct tcattaaaaa gcggaaatgg tttcatcatc tctttgactt
    23041 tttccccaac ccggttaata acctcctcat tgtcaatatt tgtcagtact tcgtcgatta
    23101 attcaacgat aagcggcata tgctcttctt ttaatcctct tgttgttatg gcgggagtac
    23161 caactctcag acctgatgtc agaaaaggtg atctggtatc gaaaggaacc atatttttat
    23221 ttacagtaat ctttgccctg acaagtgtgt tttctgcctt tttgcctgac agatccggaa
    23281 attttgttcg caaatctatc agcatcagat ggttgtcagt gcctcccgaa acgattttat
    23341 agcccttttt aatgaatgct tccgccatta ctgctgcgtt tcttttaacc tgcatctgat
    23401 attttttgaa ttgaggttcg agggcttcac cgaatgcaac ggcttttgct gctatgatgt
    23461 gctcaagcgg gccaccctgt actccgggaa aaactgcaga gtttattacc gacgacatca
    23521 tcctaatctc tcctttagga gtagtaagtc cccatggatt ctcataatcc ttacccatca
    23581 gaataatacc gcctctcggt cctctcagtg ttttatgtgt agtggaagtc acgatgtgtg
    23641 catacttcag tggattgtta agcagaccag cagctataag tccggccgga tgagccatat
    23701 caaccatcaa tactgctcca acatcatctg ctatcttcct catc
//
LOCUS       DAOSNB010000008        23404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2199_length_23404_cov_4.331663, whole genome shotgun sequence.
ACCESSION   DAOSNB010000008 DAOSNB010000000
VERSION     DAOSNB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_23404_cov_4.331663"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PLG42_00920"
     CDS             <1..486
                     /locus_tag="PLG42_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29800.1"
                     /translation="GIIVDEGKKFYTMLLTGMLIWNLFYGLIPLNRISGESEKYLCNM
                     LVENNDIAVIARDDQQIASMVFYYTGKESSHNIYKSPAVLKISGYDSEILKGVIDSLL
                     VQDKKVFTDCLSVMPMSRAVFLEGKINQQFFSAYLLKEEKRWENTGGIRIIHRITGKK
                     S"
     gene            complement(488..3361)
                     /gene="infB"
                     /locus_tag="PLG42_00925"
     CDS             complement(488..3361)
                     /gene="infB"
                     /locus_tag="PLG42_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOU29801.1"
                     /translation="MSDEVKVTRLSKAAREFNVGISTIVEFLHKKGFNLDPNPNTKLP
                     PEAYALLVKEYSTDITAKKESEKLMLKNLHRKKESVSIEDINTKTPAEESETEGEILI
                     KDSLGVTKVDIKPEISKPVVKVLGKIDLEKTVKPKVEKPEPKKDTAEEKEKKKESAEI
                     KEEAVAAEKPTKEKEAVSEAESEAKAPKATAIAEEKPAIEVPAEPVKEEKATAKAETV
                     ITSQDKSGTGAETVEQETEPEFDEEEIPTTFRLASEVKITGPVVIGKIELEEKKKPVK
                     IEYDEELKHKKRRKRISKDKEIIPLKPAEKKERERAKVFLKKRPVRAEIDEEEVQKQI
                     KETLARLTAKSKSKGVKYRREKREALILKEKEKSELQELEKKVLKVTEFVSANELAAM
                     MNVPVTDIIATCMNLGLFVSINQRLDAETMSIVAEEFGYKVQFVSAEVVEAVTEEEDR
                     PEDLKPRPPIVTVMGHVDHGKTKLLDYIRNTNVVAGEAGGITQHIGAYSVTLENGRQI
                     TFLDTPGHEAFTAMRARGAQITDIAIIVVAADDGVMPQTVEAINHAAAAGVPIVFAIN
                     KIDKPTANPDRIKEELAKMNYLVEDWGGKYQSQEISAKTGLNVNLLLEKVLLEADLLD
                     LKANPNKPAHGTVIESSLDKGKGYIATLLVKSGTLRIGDIVLAGCHYGRVKALFNERG
                     NRVTEAGPSTPVLMTGLNGAPQAGDKFNVMKTEREAKEIAIKRAQLQREQELRTQKHI
                     TLDEIGRRIAIGNFQELNLIVKGDVDGSVEALSDALIKLSTEQIQVNIIHKAVGQISE
                     SDVLLAAASNAIIVGFQVRPSMNARKLAEKEQIDIRLYSIIYDAIEEIKAAMEGMLMP
                     EVKEVIVGSLEIKEVFKISKVGTVAGCFVREGKITRDSKVRIIRDGIVVYTGELGSLK
                     RFKDDVKEVSSGLECGLNIKNFNDIKVGDIIEAYQEVEVKKTL"
     gene            complement(3416..4654)
                     /gene="nusA"
                     /locus_tag="PLG42_00930"
     CDS             complement(3416..4654)
                     /gene="nusA"
                     /locus_tag="PLG42_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOU29802.1"
                     /translation="MENLNLTDTFLEFKELKNIDRPTMMSVLEDVFRNILAKKYGSAD
                     NFDIIVNIDKGDFEIWRNRKVVADEDFTDPNTQIPLSEAKKIDADYEIGEEVSDEVKF
                     QDFGRRAILSIRQNLTARILDLEKNNLYAKYKDRIGEIVTGEVYQVWKKEILVLDDDG
                     NELILPKSEQIPTDHFRKGDTIRAVVIKVEMKNNTPVIILSRTSPVFLERLFELEVPE
                     IFDGLITIKKIVRVPGERAKVAVESYDERVDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTTNTQLYIQRALSPAKISSIKINEEAKRAEIYLKPSEVSLAIGKGGLNIKLASQL
                     TGYEIDVYREPGGEDEEDVNLEEFSDEIEEWIIDELKAIGCDTAKSVLSLSVSDLVKR
                     TDLEEETIKEVINILKAEFE"
     gene            complement(4672..5136)
                     /gene="rimP"
                     /locus_tag="PLG42_00935"
     CDS             complement(4672..5136)
                     /gene="rimP"
                     /locus_tag="PLG42_00935"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOU29803.1"
                     /translation="MIDRTKIEKLVKEYMEGKDLFLVDVRVTRDNKITVVADTPSDIT
                     IDQCAELSRFIESRLDRNEEDYELEVSSPGIGHPFIVKEQYLKNIGKSVEVVCLTGEK
                     YRGILKNVTGNGLEIESQEGKKSKSRGLTDESKNISINFEEIKSTKEILIFR"
     gene            5261..6235
                     /locus_tag="PLG42_00940"
     CDS             5261..6235
                     /locus_tag="PLG42_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOU29804.1"
                     /translation="MGYYDKIVYEMIDPGIYTEEERLILSSCTLCPRECGIDRFEATG
                     YCGADAGLNIASICIHRGEEPPISGPQGICNIFFAGCNLRCFYCQNYDISHPVSFSKY
                     AVTDLTTCVDTIEEILSKGIYAVGFVSPSHVVPQVKSIIRELGRRGYKPVTVYNTNSY
                     DKVETLKELEGLIDVYLPDYKYVSPLLAARYSDAPDYPEVAIKALKEMYYQKGSVLHT
                     DENGVAYSGMLIRHLVLPGHTDNSIGVLRSIAEEISTGVHLSLMSQYHPAGEAISMCE
                     LNRPLTKEEYSLVAEEMERLGFRNGWLQDPDSYKNYLPDFSREHPFEW"
     gene            complement(6254..7375)
                     /locus_tag="PLG42_00945"
     CDS             complement(6254..7375)
                     /locus_tag="PLG42_00945"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOU29805.1"
                     /translation="MSTEHKSVASPLPGRVASIDFFRGLTMFLLAGESTRLYEHLVKL
                     DNPVIQFIGNQFHHHEWHGLHFWDLIQPFFMFIVGVAIPFSVANRLKKGESYKQITMH
                     AIKRALLLLFLGWALYFTAAGKLVFRFQNVLAQLSVTYLVAFLLLNKSYKFQISFTVI
                     TLILIDCAYRFFPVEGFNHPWVNYENLGAWFNNKIEGVEKASEWATLNFVSTSAHTIW
                     GAMCGKLLMSEKTPSEKIRIMVIAAVIALVAGYALDLLNITPIIKKIATVSFVLVSGG
                     WTILALCFSYWLIDMKKLFLKGSKFFIIVGMNCIFIYLFFELGFTRIISNRIVAPFTN
                     LFFSWAGEMTCAIITSLGAWFILWYICYWLYKNKIFIKI"
     gene            complement(7492..8163)
                     /locus_tag="PLG42_00950"
     CDS             complement(7492..8163)
                     /locus_tag="PLG42_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29806.1"
                     /translation="MTVYNLKIIFANRFFWFLIGSVGFYSGLSLIYILSNNIYKVQDL
                     YGTFIFTGILLVFYPSVFGIQNDQDSRTIEILFGIPDYRYKVWLIRLLMIFIITFLVM
                     LVYTSISSLLIIKFRILEMTSQVMLPVMFIGTLAFMMSTVVRNGNGTAVIMIVIGIFF
                     LILSDSLSRSQWNIFLNPYDLPRDMNETAWNLITVKNRIILTTGIILCLLAGLYNLQN
                     REKFI"
     gene            complement(8205..12974)
                     /locus_tag="PLG42_00955"
     CDS             complement(8205..12974)
                     /locus_tag="PLG42_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU29807.1"
                     /translation="MDFIIKRKVLVSMLFLGLTMMGYVSYKQLPVELFPNAELPLLIV
                     QVTTPLEMDPAYIEQQAVIPVEGAIGTIEGVEKIESNVTSRYGTVIIYFNQKANLKYS
                     NLKLQEKIDIVRNSIPEEFIINVIKIDLEQITGQFMDLQVRGEGGIDRIRNIADREIK
                     PEFENLDGIAGVEVFGGRENSIEIRLNEKACKAYGVTMNQVRNLLNSYSRDKTFVGRV
                     VDGANRYFVNITSEYTDVSEIGNIIINKEGPVLLKDIAEIFFGVKEETSYSRINGMDA
                     VTITLVNDNQANTIDLSHKAAALVNSLNQKLKPLGVEIVIQNNTTEVLERNINQIISL
                     AITGGILAIVVLWFFLRNLRLVMIIGVSIPVSVYTAFNFFYGAGISINSLTLVGMALA
                     VGMLVDNSVVVLENIYRLSGQGFIPEQAVKTGTREVRKAIIAATLTTIIVFTPFVFSG
                     NYLIKIIGKNIGVSIISTLSVSLLAALMLIPMATYYLLNVKGGRSEIFKNLSIHNRLV
                     QAYHLILKACMRKPAATIIGTLVVFFAALLTSLTLSISSTREVDTPNFRLSVRMPSGS
                     TLEKTDAVVAEIESRLATLPEKGDVISRIEEEQATVTVTLPDDWKKKTKRSLPEIKND
                     ISERVKNITQAEITMDEISAGGGYSSGGAGGGTNPGASFLNLLGIGTQRESIIIKGQN
                     FDQMKILADDIKSYLDDLSTISSSSVNVQDNRPEVQLHFDMDYINRNNFTLANLATAL
                     STFGREYSSGARFRQGTETYDIILKYNDEAAQENQNSEKTLEDLKHLEVTNPTGSVME
                     LQELARIVFSAGPGNIHRENQEKRVTVTYNFTEEIRNSKDLLESARAEVEAIVADLNI
                     PSGIAVEVVHEENELKEFYKLIGIAFVLIYMILAAVFQSLTVPVVLLFSIPLAALGSL
                     LALILTGNSLLNANTLTGFLILLGIVVNNGIILIDYTKTLRNRGYRRSRALMMAGVAR
                     IRPIMITALTTILALLPLAMGQAEYVSVIGASFAITVIGGLSLSTMLTLVFIPTFYSG
                     LENAIDWIKSLSTRTKIIQLCALAALLYLVFTFVNTFIWQLITTILSVIIVPAATWFI
                     IYSLRKAKESVIPQNEPINIQIQSLVKIYDRESRWLREWKAGARIRRRLGLEKEIRSL
                     KDMENMTWQLPLFGFLIYFTYFYLSKGFWVFFFSIITWLALFGIWSSFRQFLTNLASK
                     NQKLPVKNISRIIEFIVMWFVPLFNLVFFQIQWKNIATVLILGFVWYASILVYITGEK
                     LTRENIEIYRLQGRFRELRKTFYKIVLMVPLIGKRKKPFKALNCVSMTIGSGMFGLLG
                     PNGAGKTTLMRIICGILEQSYGKVWINGIDTQKKREELQGLIGYLPQEFGTYENMTAH
                     EYLHYMAILKNITDPEVREERVTYALNAVHMADRRFEKISSYSGGMKQRIGIAQILLH
                     LPRILVVDEPTAGLDPRERIRFRNLLVELSRERVVIFSTHIIEDISSSCNKVAVLDRG
                     NLKYLGRPIDMAVSAEGHVWQITMHASEFKEFIKDHLVVHHMSEGNMVKLRCISEEKP
                     WETAIKVTPNLEDAYLWLLRRKNNGENILPETNRLMQ"
     gene            complement(12991..16119)
                     /locus_tag="PLG42_00960"
     CDS             complement(12991..16119)
                     /locus_tag="PLG42_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU29808.1"
                     /translation="MNRIARFAVRYPVTVLMFVLGVMLLGFISFGKLGTDLFPDLNNP
                     KIYIEIKAGEKPPEEIEKNYVDQIESIAMRQNGVIQVSSSSKVGKAVVTVEYDWKKDM
                     DEAFLDLQKELNSFAQNSDIEDFTMSQYDPNAAPVMVVALKNERLNNLDELRKVAENY
                     IRNELVRIDGVADVKLAGIEQSEVIIETKKHILESYGITSDAIAAQISNYNRNVSGGS
                     IVEMGTRYVVKGVSVLRDLTDLENIIVGFKQTTSATSAGIAASASSSSRVPIYLKDVA
                     KIYFGNKEPDNIVTINGERCIGLSIYKEPKFNTVEAVKKLKEEFANLSRALPGYEFIF
                     IQDQGKYIRSAITEVQNTALIGIVLAVFVIYIFLRRIGATLVISLAIPISIIATFNLM
                     YFNHLTINVMTLGGLALGAGMLVDSAIVVLENISRNREEGMPFIDAIITGTGQVGGAI
                     TASTITTIVVFLPIVYIHGASGELFKDQALTVAFALLSSLFVALLVIPMLVSTIFKEK
                     SARAISASVKFGWYSKILEKIVEKRGIVLISAFIIMGLTALLIPKLGSEFMPGAESNQ
                     FNIEIKLPEGTSLERTASTTERIEKLIRDILGDRIEAIYAQAGATGTSYYTESGADEG
                     ENISNLKVILKNEYVSQSDESMALVESFLKNIPEMEVTFSREETALQSTLGTTGPPFA
                     LEISGTDYEEIEKIMNQAKEILSEVKGLYNITTSLDEGSPQVEISIDRFRTSYYNVSA
                     ENIVNQVRSYLTGASAGSFEKDGELKDITIKLEDITLNQLKDLMITAGSVKVPLSELA
                     NIKIVTTPKEITRRNQSRTGYIYAMVNKDIPFDQLIKTAGEALRKITLPPDYKMEFTG
                     EELKRKESMSNLTFALILSLVLVFMVLAAQFESLIQPFIIMLTIPLAGVGSILTFLLL
                     GKSLNMMAYIGIVMLAGIAVNNAIILVDRINQLRAEGMTRREAIITAGTQRIRPIIMT
                     SLTTILALLPLTLGIGESASLRSPMALAVIGGLFTSTLLTLAVIPCAYWVTDAAGEYI
                     TGKTFRKTIKTV"
     gene            complement(16125..17183)
                     /locus_tag="PLG42_00965"
     CDS             complement(16125..17183)
                     /locus_tag="PLG42_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU29809.1"
                     /translation="MKRSIFLIVIAFAILSLAGCRNQEPNLSADIEIPVTVEDIRLKS
                     IEEYVSTTGIVYPHGEVTLKAKISASYYLEKNPQTGRPWQLGDRVKAGSLIARLEDKE
                     FILSIKLETNELNLELAESELRKQESLYEKGGVTLKDLKTAGINYENAKTAVENARLQ
                     LEKTRVVSPIDGIIVELPYYTPGTQVEAGSTIAKIMDYQTMYMNVQLPEKYIGVIKPE
                     QPVKLTNYTIPEDTLYGKVTQLSPAINPDTRTFQGVITVNNPDLLLRPGMFVKADIII
                     NRKDSVIVIPKSIILSRQRGKTVFVVDRGLALERIIETGLENLSEAEVVRGLSKNERI
                     VTSGFETLSNRSRVKIVK"
     gene            complement(17202..18686)
                     /locus_tag="PLG42_00970"
     CDS             complement(17202..18686)
                     /locus_tag="PLG42_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU29810.1"
                     /translation="MKTKPLNTKPLLLFICMISALELPCHAQKGLTLQDAMKIAEANS
                     PSIKQTKLSLIRSQENLNAQNAALKSNFSLTINPIGYTQSREFNDLISKWNTRRSTES
                     YSVFTVSQPILFTDARVSLVNRFGYKDSYSEFTNATTKGFSNNLSINLDQPLFTYNRT
                     KLQLKQLQLALENAQLNYALQLLSLERQVAQAYYYVYQQQQSLEIAKQAYENMKQSYE
                     ISKNKVEAGLSAQDELYQAELNLATTKSDFENKQVSLENAKDDFKILIGMDLYDDFVV
                     LPDISVDTIPVDIAFAIDHGLKNRMELRQREISIENSQFELIQTKALNEFKGNLSLSV
                     GLFGDNEKFSDVYANPTDNESVALSLTIPLWDWGEKKSRIKATEASIESAKISFENEQ
                     NNIILGIRKIYRNLINLQNQIEIARKSVTNAQLTYELNLERYKNGDLTGMDLNIYQNQ
                     LSERQLAYTNALISYKLELLNLKIQTLYDFEKKEPVSPVLKLNQ"
     gene            complement(18770..22294)
                     /locus_tag="PLG42_00975"
     CDS             complement(18770..22294)
                     /locus_tag="PLG42_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29811.1"
                     /translation="MTFLHNILTVAHYEAKTLRRTWFFRLFAIGALFIFTMLNIGLFS
                     PVGAESWEMVSIPSALPLINLYLLNIGQAVVVIFLASDFLKRDKKLDTVEVLYTRSVS
                     NFEYVTGKTLGILRLFLSLNVVILLIGLIINIISKTMKVDILSYFEYLLFISIPTLIY
                     SLGLAYILMLIIGNQAITFFLLLGYAALNMFYLWYRAGSLYDYMVFGMPLYKSGIIGF
                     DNIFSIINQRLGYFFAGVSMIMASVILFKRLPQSRLHTNLARTLLVLCIIGSGITFYN
                     TLNNHLEREKLKKSIIEINRKYEKSKFPSTTSADIELAHKGKTIEAVSLLRIVNDNDT
                     PLESYIFSLNPGLSVEKVISGNSNIQFKTDGHIIEISPAKRLEPGGYDSVTIFYSGKI
                     TEAFCYPNFSDDPKNNRYRIAMVNVNKRQAFLKNNYVLFTPESHWYPVSGLNYYPSNP
                     ARIKVDFTKFILKAKSDKNLKVVSQGVPGEEGEWTKYSCPFPLTGLTVAMGNYVADSI
                     TVDSVKFISWHYPGTDYYKKDFEELKDTLPNLISGFMKDLESGFGMAYPFSCLSMVEA
                     PVQFYSYPKSNTQTRAEIQPSMVIIPEKLSTLQQAGFAKRFARQKKQMARNNQIITDK
                     ELKVRIFNNFIRNTFISGRNFRMVNGVLVNEPTRYLLGPSFYFFRNNFYSDEYPVINA
                     VFESHLQKLNEPAPAGGPGMMAGNLSDNDRANLILKNTSFKELLARNPSSDTMRAVLT
                     LKGDCLFNYLRYKAGINEFNEWFKNYIWDNSYKRVNILKFNNDLKERFGFEFYPYLEE
                     WFNGREQPGFIFTEPDVTTIVVDQRTRFQVVFTASNTESAGGLFNVSFRTGGGFGGRG
                     GGQATMAVTFQGGRGGASGGGRIMQIAMQGRGLASSDISKIVYLSPHETKKIGVVLES
                     QPRAMQINTLFSKNIPGELVFTIQEVKKESAKQKPFEGEDIIQEIPSFTQPGEVVVDN
                     EDPGFYRSATSKMNPLRKILSGNKEVAETYQSMNLFVAPEYWQPVVQNTFYGRFVRSA
                     VYTRAGTGDRYVTWKGIIKEPGYYDVYAYIGKAGAQIIMRGAGRPGQAGAQPGRPPAG
                     GPPGTPPPPGDQSGEPARQNPYKDFHFKVYHDDGMDEITIDYETADPGWNKLGTFYLS
                     PDTVKVEMTNQSEGRVVIADAVKWVLKE"
     gene            complement(22306..23202)
                     /locus_tag="PLG42_00980"
     CDS             complement(22306..23202)
                     /locus_tag="PLG42_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU29812.1"
                     /translation="MKIEIRDLNKIYPNGTHALKNINLVIESGMFGLLGPNGAGKSTL
                     MRILVTLVKPTSGVVLLDGKDIQKHRKEVRKILGYLPQDFRFFTSLKTWEFLDYSAAL
                     AGMKNRKERLKEVDRMLDQVGLLEVRDRNANKLSGGMKRRLGIAQALIGNPKLIIVDE
                     PTTGLDPDERIRFRNILSTLSQKQDVIIILSTHIVGDISSTCQNMALLNKGEVVFCGR
                     PENLVNEAKGHVFKMNLTPYEYEKAKEEYDIISTIPVENGWEVQIVSDLPPDGNAVEI
                     EPNIEHAYVYYMEHKIHADLNV"
BASE COUNT         6224 a         4829 c         4628 g         7723 t
ORIGIN      
        1 ggcataattg ttgatgaggg taaaaaattc tacacaatgc ttttaacggg aatgctgata
       61 tggaatctgt tctatggttt gattcctctt aaccggatat caggtgaatc tgaaaaatat
      121 ttatgtaata tgctggttga aaataatgat attgctgtta ttgccaggga tgatcagcag
      181 attgcttcaa tggtttttta ttatacaggc aaagagagtt cacataatat ttacaagtca
      241 ccggctgttt tgaaaataag tggctacgat tctgaaatat taaaaggagt tattgatagt
      301 ttgcttgttc aggataagaa agtttttaca gattgcctaa gtgtaatgcc aatgtcgagg
      361 gcagttttct tagaggggaa aattaatcag caatttttca gtgcttacct attaaaggaa
      421 gaaaaaagat gggagaatac tggaggaatc cgtatcatcc atcgtattac agggaaaaag
      481 agttgattta aagagttttc ttcacttcaa cttcctggta agcttcaatg atatcgccca
      541 ctttgatgtc gttgaagttt ttgatattta agccgcattc cagtccgctg gaaacttcct
      601 ttacatcgtc tttaaatcgt ttcagtgagc ccagttcacc ggtgtaaacg actataccat
      661 ctctaataat gcggactttt gaatctctag taattttacc ttctctcacg aagcaacctg
      721 cgacggttcc aaccttgctt attttaaaga cctcttttat ttcgagagat ccgacaatta
      781 cttctttaac ttctggcata agcatgcctt ccattgcagc tttgatttct tcaatagcgt
      841 cgtaaattat tgagtaaagg cgtatatcaa tttgttcttt ttcggcaagt tttcgggcat
      901 tcattgaagg tcttacctgg aagccaacaa tgattgcatt ggaagcagca gccagcagga
      961 cgtctgattc agagatctgg cctacagctt tatggattat gttgacctgt atctgttcgg
     1021 tcgacagctt gattagagca tcactgagtg cctccacgga gccgtcgaca tcacctttaa
     1081 caataagatt gagctcctgg aaattgccga tagctatgcg gcggccaatt tcgtcgagag
     1141 taatatgttt ctgggtgcgg agttcctgtt ccctttgcag ctgagccctt tttattgcta
     1201 tttccttagc ttcgcgttcg gttttcatca cattgaactt gtctcctgcc tgtggagctc
     1261 cgttaagtcc agtcataaga acaggtgttg aaggccctgc ctcggtaact ctgttccctc
     1321 tttcattgaa cagtgccttt actcgaccat agtgacaacc tgccagaaca atatcaccga
     1381 tacgtagagt accgcttttt acaagtaatg tggcaatata acctttccct ttatcgagcg
     1441 atgattcaat aactgttcca tgagccggtt tgttgggatt ggctttcaag tcgagcaggt
     1501 cggcttcgag aaggactttt tcgagaagaa ggtttacgtt gaggcctgtc ttggcagaaa
     1561 tttcctgcga ctggtatttt cccccccagt cctcaacaag gtaattcatt tttgcaagtt
     1621 cttcctttat tctgtcaggg tttgcagtgg gtttgtcaat tttgtttatt gcaaaaacta
     1681 ttggaacacc cgcagcagca gcatggttta ttgcttctac agtctggggc ataaccccgt
     1741 cgtcggcagc aacaacaatt attgcgatat cggtaatctg ggcgccgcga gcacgcatag
     1801 ctgtaaatgc ctcgtgaccg ggcgtatcga gaaatgtgat ctgtcttccg ttttcgagtg
     1861 ttacactgta tgcgcctata tgctgggtga tacctcctgc ctcacctgcc acaacattgg
     1921 tattgcggat atagtcaagc agctttgttt tcccatggtc aacatgtccc attacagtca
     1981 caatgggtgg cctgggctta aggtcttcag gtctgtcttc ctcttctgtt actgcttcta
     2041 ccacttcggc acttacaaac tgaaccttat aaccgaactc ctctgcaaca attgacatgg
     2101 tttcagcatc caggcgctgg ttgattgata caaagagccc cagattcatg caggtggcta
     2161 tgatgtcagt aacgggaaca ttcatcatag ccgcaagttc gtttgccgaa acaaattcag
     2221 taaccttaag aactttcttt tcaagctcct gaagctccga tttttccttt tccttaagga
     2281 ttaatgcttc cctcttttcc ctcctgtatt ttactccttt tgattttgat tttgcagtga
     2341 gccttgccag cgtctccttg atttgcttct gtacttcctc ttcgtcaatt tctgccctga
     2401 caggtctctt tttcagaaat acttttgctc tttctctttc tttcttttcg gcaggtttca
     2461 gaggaataat ctctttgtcc tttgaaattc tttttcttct ctttttatgt tttagttctt
     2521 catcatattc gattttaaca ggtttctttt tttcttccag ctctattttg cctatcacca
     2581 cgggccctgt tatcttgact tcagatgcga gtcggaaagt agttggaatt tcctcttcgt
     2641 caaattccgg ttccgtttcc tgttcaacag tttcagcacc tgtacctgat ttatcttgtg
     2701 atgttataac ggtttcagct tttgctgttg ccttttcttc ctttacaggt tcagcaggta
     2761 cttcaatggc aggtttctcc tctgcaatgg cggtagcttt tggagccttt gcttcggatt
     2821 cagcttcact gacagcctct ttttccttcg ttggtttttc tgcagctact gcttcttcct
     2881 ttatctcggc agactctttc ttcttttctt tttcttctgc tgtatctttt ttgggctcag
     2941 gtttctcaac ctttggtttt actgttttct caagatcaat tttacccagt acttttacaa
     3001 caggtttgga tatttcgggt ttgatatcga cttttgtcac cccaagcgaa tctttgataa
     3061 gaatttcacc ttctgtttca ctctcttcgg ctggtgtttt ggtattaata tcctcaatag
     3121 aaactgactc tttttttcta tggaggttct taagcattaa tttttccgat tctttctttg
     3181 ccgtgatgtc agtactgtat tccttaacaa ggagggcata agcttcggga ggtagtttgg
     3241 tattgggatt tgggtcaaga ttaaaccctt tcttatgcag aaattccaca atagtggata
     3301 ttcccacatt gaattcgcgg gctgctttac ttaatcttgt aacctttact tcgtcactca
     3361 tagaaatcct cctgtaatat tctcctctga tatataaatt taaaatcttg tttttttact
     3421 caaactctgc tttcagtata ttgattacct ctttgattgt ttcctcttca aggtctgttc
     3481 tcttcacaag gtcgcttaca gaaaggctga gaacactttt tgccgtatcg cagccgattg
     3541 ctttaagttc atcgattatc cattcttcaa tttcatcgga gaattcctcg aggttgacat
     3601 cttcctcatc ttcacctcct ggttcgcgat agacgtctat ttcgtaacct gtaagctgac
     3661 tggcgagttt tatattcaat ccgccttttc caatagccag agatacttca ctcggtttca
     3721 ggtagatttc agccctcttc gcttcttcgt ttattttaat agaggatatt tttgccgggc
     3781 taagggctct ctggatatat aactgcgtat ttgttgtata atttatgaca tcaatatttt
     3841 cgtttctaag ttccctgact atcccatgta ttcttgatcc tttcatgccc acgcatgctc
     3901 ctacaggatc aactctttca tcatatgatt caactgcaac cttagcacgc tcaccgggta
     3961 cccttacaat ctttttaatg gtaatcagac cgtcgaaaat ttccggaact tcaagttcga
     4021 aaagcctttc gaggaatacc ggtgaagtac ggcttagaat aattaccggg gtgttgtttt
     4081 tcatttctac ttttataaca acagccctta tggtgtcacc cttccggaaa tggtcagtcg
     4141 gtatttgctc gcttttcggt agaataagct cattaccatc atcatccagc acaagtatct
     4201 ctttcttcca tacctggtaa acttcgcctg tcactatttc accaatccgg tccttatact
     4261 tggcatatag attgtttttt tcaaggtcaa gaatacgggc agtaagattc tgacggatgg
     4321 aaagtatagc tctcctcccg aaatcctgaa attttacttc atccgaaacc tcttcgccta
     4381 tctcgtaatc ggcatctatc ttttttgctt cagaaagagg tatctgagta ttagggtcgg
     4441 taaaatcctc atcagcaaca acttttctgt ttctccagat ctcaaagtca cctttgtcga
     4501 tattaacaat aatatcgaag ttatcggccg agccatactt ctttgcaagt atgttcctga
     4561 aaacatcttc aagaacactc atcatcgttg gccggtcgat gttttttaat tccttgaact
     4621 ccagaaaagt gtctgttaaa ttaagatttt ccatctttca cattctcctt tttatctaaa
     4681 aattaaaatc tcctttgttg atttaatttc ttcaaagtta attgatatat tttttgattc
     4741 gtcagttaaa cctctgcttt tgctcttttt tccctcctga gattcaatct ccagtccgtt
     4801 tcctgtaaca tttttcagta tccccctgta tttttcccct gtcaggcata ccacttcaac
     4861 acttttcccg atgtttttta aatactgttc tttaacaatg aaaggatggc cgatacctgg
     4921 agacgacact tcaagctcat aatcctcttc attgcggtcg agcctgcttt cgatgaatct
     4981 gctcagctca gcacactggt caatagtaat gtcagagggt gtatctgcaa caacagttat
     5041 tttattgtca cgcgtaaccc tcacatctac aaggaaaagg tctttacctt ccatgtattc
     5101 tttgaccagt ttttctattt ttgttctgtc aatcattaca aaaaacataa taaaacaggg
     5161 ggactcatcc ccctggtgtt tttaacgcca cgaatcaggt gcaaatataa taatattttt
     5221 ggaattatca aaatctgaat agttttacaa aattttctga gtgggttatt atgataaaat
     5281 agtgtacgaa atgattgacc cgggaatata tactgaggag gaaaggctaa tactttcttc
     5341 gtgcaccctg tgcccaagag aatgtgggat tgacaggttt gaagccaccg gttattgcgg
     5401 ggccgatgcg ggccttaata ttgcctctat atgtattcac aggggagaag aacctcctat
     5461 aagcgggccc cagggtatat gtaacatatt ctttgcagga tgcaatttga ggtgttttta
     5521 ttgccagaat tatgacataa gccatccggt aagcttttcg aaatatgccg ttactgattt
     5581 aacaacatgc gtggatacta ttgaagaaat tctttcaaaa ggtatttatg cagtgggatt
     5641 tgtttcacca tcgcatgttg taccacaggt taagtcgatc atcagggaac tgggaaggcg
     5701 agggtataaa ccggttacag tatataatac aaatagttac gataaagttg aaactttaaa
     5761 agagcttgaa ggtctcattg atgtttacct tccagattat aaatatgttt cacccctttt
     5821 agctgcaagg tattctgatg ctccggatta tcctgaggta gcaattaaag cacttaaaga
     5881 gatgtattat cagaaaggtt ccgttttgca tacagatgag aatggcgtag cttacagcgg
     5941 gatgttgata cgccatcttg ttcttccggg gcatacagat aatagtatag gggtattgcg
     6001 tagtattgca gaagagattt caacaggcgt ccatctttca cttatgtcgc aatatcatcc
     6061 tgcaggtgag gcaatatcaa tgtgcgaact taaccgcccg ttaacaaaag aagagtacag
     6121 cctggtggct gaggagatgg aaaggctggg attcagaaac ggatggctgc aggatccgga
     6181 tagttataaa aactaccttc cggatttctc aagagaacat ccgtttgagt ggtaaaaagt
     6241 tcctgagttt ctgctatatt tttataaaaa tcttgttctt ataaagccag taacagatat
     6301 accagagtat aaaccatgcc ccaaggcttg taattattgc acatgtcatt tcaccagccc
     6361 atgagaaaaa cagattggtg aaaggagcta ctatccggtt gcttattatt cttgtaaagc
     6421 caagttcaaa aaacagatat ataaagatgc agttcattcc cacaattatg aagaattttg
     6481 aacctttcag gaaaagtttt ttcatgtcaa taagccagta gctgaagcag agggcaagta
     6541 tagtccatcc gccgcttaca agaacaaacg atactgtagc tatcttttta ataatcggtg
     6601 ttatattaag caagtcaaga gcatatcctg ctacaagtgc aataactgca gcaatgacca
     6661 taatacgtat tttttccgat ggtgttttct cgctcataag cagtttaccg cacattgcac
     6721 cccatatggt atgggctgaa gtggaaacga aattaagagt tgcccattca cttgctttct
     6781 ccacaccttc aatcttattg ttaaaccagg ctccaagatt ttcataattt acccagggat
     6841 gattgaaccc ttccacggga aagaaccggt aagcacaatc gataagaatg agggtaatga
     6901 ctgtgaagga tatctgaaat ttgtaactct tgtttaaaag cagaaatgct acaaggtatg
     6961 ttactgacag ttgtgccagt acattctgga atctgaaaac aagttttcct gctgccgtaa
     7021 aataaagagc ccatccaaga aagagcagaa gcagagccct tttgatagca tgcatggtaa
     7081 tctgtttgta gctttctcct ttcttcagcc ggtttgcaac agagaaaggt attgccacac
     7141 cgacaataaa catgaaaaaa ggctggataa gatcccagaa atggagtccg tgccattcat
     7201 gatgatgaaa ctggtttccg atgaattgaa taacaggatt atccagtttt acaagatgtt
     7261 cataaaggcg ggttgactcg cctgccagaa gaaacattgt tagtcccctg aagaaatcta
     7321 ttgaggcaac tctgccggga aggggagagg caacactttt atgttctgtg ctcactttat
     7381 tagtgttttt ggttaatgca aagagctaaa aatttcaatc tctcaaaata aaaatttttt
     7441 ctgaattgat tattacttca gaaaaataat tatttacagt gatttggttt attaaatgaa
     7501 tttttctcta ttctgaaggt tgtatagacc ggccagcagg cacaggataa ttccagttgt
     7561 gaggataatt ctgtttttta ctgttataag attccatgct gtttcgttca tgtcacgggg
     7621 aagatcgtaa ggattcagaa aaatattcca ctggcttctc gatagactgt cggatagtat
     7681 caaaaagaag atgcctataa caatcattat tactgcagtg ccgttaccgt tccgtactac
     7741 tgtcgacatc ataaaagcca gtgttccgat gaacattacc ggaagcataa cctgggaggt
     7801 catttcaagt atcctgaatt ttattattaa taatgatgaa atggacgtat aaacaagcat
     7861 taccagaaaa gttattataa atatcattaa aagccttatc agccatactt tgtaacggta
     7921 atcgggtata ccgaaaagaa tttcaattgt tctggagtcc tggtcgttct gaataccgaa
     7981 aactgaagga taaaagacca gcagtatacc cgtaaagata aaagtaccgt agagatcctg
     8041 aactttataa atgttatttg agaggatata tatcagtgat agtcctgagt aaaatccaac
     8101 tgagccaatc agaaaccaga agaatctgtt tgcgaaaatg attttcagat tataaactgt
     8161 cattttctga atcacgttaa aatatttata aagtatattt cttttcattg cattagtctg
     8221 tttgtctccg gtaatatgtt ttctccgttg tttttccttc tgagtagcca caggtaagca
     8281 tcttcaaggt taggcgtgac tttaatggct gtttcccatg gtttttcttc agatatgcat
     8341 ctcagtttaa ccatattgcc ttccgacata tggtgaacga ccagatgatc ttttatgaac
     8401 tctttgaatt ctgatgcgtg catagttatt tgccagacat ggccctctgc acttaccgcc
     8461 atatcaatag gtcttcccag atattttagg ttacctctgt cgagtacagc aaccttattg
     8521 catgaacttg agatatcttc gattatatgg gtggaaaaga taactactct ttccctgctg
     8581 agttcaacaa ggaggtttcg gaaccttatt ctttctctcg gatcgagacc tgcagttggt
     8641 tcatcaacca caaggattct cggaaggtgg agcaaaatct gagctattcc tattctctgc
     8701 ttcatgcctc ccgagtacga actgattttt tcaaatcttc tgtcggccat atgaacggca
     8761 ttaagggcat aagtgacgcg ctcttccctc acctcagggt cggttatgtt tttgagtatt
     8821 gccatataat gaagatattc gtgggcggtc atattctcat aggttccgaa ctcctgaggc
     8881 aggtagccta tcaaaccctg aagttcctct cttttcttct gggtgtcaat tccgttaatc
     8941 cataccttgc cgtagctctg ttcaaggata ccgcaaataa tgcgcataag ggttgtcttg
     9001 cctgccccgt taggcccaag caatccgaac attccacttc cgatggtcat gcttacacag
     9061 ttgagagcct tgaagggctt tttccttttc ccgataagag gcaccattaa aactatttta
     9121 taaaatgttt ttctgagttc cctgaaccga ccctgcagtc tgtaaatttc tatgttttcg
     9181 cgcgtaagct tctctcctgt tatataaaca agaatggaag cataccatac aaaaccaaga
     9241 ataagcactg ttgcaatgtt tttccactga atctgaaaga aaaccagatt aaacagtggt
     9301 acgaaccaca taacaataaa ttcgattatc cgggaaatat tttttacagg aagtttttga
     9361 ttcttacttg ccaggttggt taaaaattgg cggaatgagg accatattcc gaatagtgcc
     9421 agccatgtga taatactgaa aaagaatacc cagaatccct ttgaaaggta aaagtacgtg
     9481 aaatagatca aaaagccgaa tagtgggagt tgccatgtca tattttccat atccttcagg
     9541 gagcgtatct ctttttcgag tccgagtctg cgtctgattc ttgctcctgc tttccattcc
     9601 cttaaccatc ggctctccct atcgtatatt ttaacaaggc tctgtatctg gatgttgatt
     9661 ggctcgttct gaggaataac actttctttt gctttgcgca agctgtagat aatgaaccat
     9721 gtggcagcag gaacaattat aacagataga attgttgtga taagctgcca gatgaaggta
     9781 ttgacaaatg tgaacaccag atagaggagg gcagcaaggg cgcagagctg aataattttg
     9841 gttcttgtgc tgagagattt gatccagtcg attgcatttt cgaggcccga gtaaaatgtg
     9901 ggaataaaaa caagtgtcag cattgtagaa agtgacaatc cacctattac tgtaattgcg
     9961 aatgaagcgc cgattactga tacatattct gcctgtccca ttgcaagagg cagaagtgcc
    10021 agaatagtgg taagggctgt aatcataata ggccttattc gtgccactcc tgccatcatc
    10081 agtgctcttg acctgcggta accacgatta cgcagagttt ttgtgtaatc gataagtatt
    10141 atcccgttat ttaccactat ccccagcagg ataagaaaac cggtaagtgt attggcattg
    10201 agaagggagt taccggtgag aattaatgcc aggaggcttc cgagggcagc gagaggaatg
    10261 ctgaaaagca acactaccgg aacagtaagc gattgaaaca ctgctgcaag aatcatgtaa
    10321 ataagtacaa aggctatgcc aataagcttg tagaactctt tcagctcgtt ctcttcatgc
    10381 actacttcta cagctattcc ggatggtatg ttgaggtcag ccacaattgc ctccacttct
    10441 gccctggctg actcgagaag atcttttgaa tttcttattt cttctgtgaa attgtatgta
    10501 acagtaactc ttttttcctg attttcccgg tgtatattgc ccggtcctgc agaaaagacg
    10561 attcttgcca gttcctgtaa ctccatcaca gagccggtgg ggtttgttac ttcgaggtgt
    10621 ttaaggtctt cgagggtttt ttctgaattt tggttttcct gtgctgcttc atcgttatat
    10681 ttcagtatta tatcgtaagt ttctgttccc tgacggaatc ttgcacctga tgaatattct
    10741 ctgccgaagg tgctcaatgc tgttgcaagg ttggcaaggg taaaattgtt tctgtttata
    10801 tagtccatgt cgaaatgaag ctggacttca ggtcggttat cctgtacatt gacacttgag
    10861 ctgctgattg ttgagaggtc gtcgagatat gatttgatgt catcggcaag aattttcatc
    10921 tggtcgaagt tctggccctt tattattata ctttctcgtt gagtgcctat acctagcagg
    10981 ttaaggaagc tggcaccagg atttgttccg ccgcctgcac cccccgaact gtatcctcct
    11041 ccggcggaaa tctcatccat ggtaatttca gcctgagtaa tgtttttaac cctttcactt
    11101 atatcgtttt tgatttcagg cagacttctt tttgtctttt tcttccagtc gtcaggtaga
    11161 gttactgtta ccgtagcctg ctcttcttca atgcgactta ttacatctcc tttttcaggt
    11221 aatgtggcaa gacgcgattc tatctccgca actactgcat cggttttctc taaagtggag
    11281 cctgagggca tcctcaccga gagcctgaaa ttcggagtat ccacttcccg ggttgagctt
    11341 atacttaatg tgaggcttgt cagaagagct gcaaagaaaa ccacaagagt gcctattatt
    11401 gtagcggccg gttttctcat acaggctttg agtatcagat gataagcctg aaccagcctg
    11461 ttgtgtattg aaagattttt gaaaatttcc gacctgcctc ctttaacatt aaggagataa
    11521 tatgtggcca tcggaattag cataagagct gcaagcagcg aaactgaaag tgttgaaatt
    11581 atcgaaacac ctatattctt tccaattatt ttaataaggt aattgccaga aaacacaaaa
    11641 ggtgtaaaaa ctataattgt agttaatgtt gcggctataa ttgctttcct gacttctctg
    11701 gtaccggtct tcacagcttg ttcaggaata aaaccctgac ccgacagcct gtatatattt
    11761 tcaagtacaa caaccgaatt gtccaccagc ataccaactg caagagccat tcccaccagt
    11821 gtgagactat ttattgatat ccctgctcca tagaagaaat taaaagctgt atatactgat
    11881 acgggaatag atactcctat tatcataaca agcctgaggt tacgcaggaa aaaccataaa
    11941 accacaatag caagaatgcc tcccgtaata gccaggctaa ttatctgatt gatatttctt
    12001 tcaagtactt ctgtcgtgtt gttttgtata actatttcaa caccgagcgg cttaagtttc
    12061 tgattgagac tgtttacaag tgcggcagct ttatgagaga gatctattgt gtttgcctga
    12121 ttgtcattta ccagagtaat tgtgacagca tccattccat ttatcctgct gtatgaggtt
    12181 tcttccttta caccaaaaaa tatttcggct atgtctttta gcaggacagg accttcctta
    12241 ttaattatta tatttccgat ttcggagacg tcagtatact cggatgtgat attgacaaag
    12301 tacctgttgg caccatcaac aactcttccc acaaaagtct tgtctcgaga ataactgttc
    12361 agcagatttc ttacctggtt cattgttacg ccgtatgcct tgcaggcttt ttcgttgagc
    12421 cttatttcga tagaattttc cctgcctccg aatacttcca caccggcaat tccgtcaaga
    12481 ttttcaaatt ctggctttat ttcacggtcg gcaatattcc ttatacggtc aattccaccc
    12541 tcgcctctta cctgaaggtc catgaattga cctgtaatct gttccaggtc tattttgatg
    12601 acattaataa taaattcttc gggaatggaa tttctaacta tgtcaatttt ctcctgcaat
    12661 ttcaggtttg aatatttcag atttgctttc tggttgaaat agatgataac tgtaccataa
    12721 cgtgaagtga catttgattc aattttttcc actccttcaa tagttccaat cgcaccctct
    12781 accgggatga ctgcctgttg ttcgatgtaa gcaggatcca tttccagagg agtggttacc
    12841 tgtacaatca gaaggggtag ttctgcattt ggaaatagtt ctaccgggag ctgtttgtat
    12901 gaaacatagc ccatcatcgt gagccccaga aagagcatac ttacaagtac cttcctttta
    12961 atgatgaaat ccatttttta tcattataaa tcagacagtt ttgattgttt tcctgaatgt
    13021 tttacctgtt atgtattctc ctgctgcatc ggtcacccaa taagcacatg gtataacggc
    13081 cagagttagt aatgttgagg tgaagagtcc accgatcacc gcaagggcca taggtgaacg
    13141 caacgaggca ctctctccta tccctaatgt caggggtaat aaagcaagaa ttgtagtcag
    13201 gcttgtcatt attattggtc tgatcctctg tgtccctgcg gtaataatag cctctctcct
    13261 tgtcattcct tcagctctta actggtttat cctgtcaaca aggataattg cattgtttac
    13321 ggcgatgccg gcaagcatta ctattccaat gtaagccatc atgttaagtg actttcccag
    13381 aagcaagaag gttagtattg agcctacacc tgctagaggg attgtaagca ttataataaa
    13441 aggttgtatc agcgactcaa actgtgcggc aaggaccata aaaactagca cgagagaaag
    13501 tatcagtgca aatgtgagat ttgacattga ttctttacgt ttgagttctt ctccagtaaa
    13561 ctccattttg tagtcggggg gcagggtaat ttttctaaga gcttctcctg ctgtctttat
    13621 taactggtcg aaaggtatat ccttgtttac catagcgtag atgtagcctg tgcgtgactg
    13681 gtttcttcgg gttatctctt ttggggtagt tactattttt atatttgcca gctcgctgag
    13741 tgggactttt acgctcccgg cagtaatcat aaggtccttc aactggttca gggtaatatc
    13801 ttctaattta attgtgatat ctttcagttc accgtctttt tcgaaactgc ctgctgatgc
    13861 tcctgtaagg taacttctaa cctggtttac aatattttct gccgaaacat tataataact
    13921 tgttctgaag cggtcgattg aaatttccac ctgcggagaa ccttcatcca gcgaagtggt
    13981 aatattatac aggcctttaa cttcggaaag gatctctttt gcctggttca taattttttc
    14041 gatttcctca tagtctgttc ctgagatttc cagagcaaat ggtggaccgg ttgtacccag
    14101 agttgactga agggcggttt cttcgcgtga aaatgtaacc tccatttcag gtatattctt
    14161 cagaaagctt tcaacaagtg ccattgattc atccgattgt gaaacatatt catttttaag
    14221 aatgactttc agattagaaa tattttcgcc ttcatcagca cccgattcgg tatagtaaga
    14281 agtgcctgtt gctccggcct gggcataaat ggcttctatt ctgtctccca gaatatctct
    14341 tattaatttt tcgattcttt cagttgttga tgctgttctc tccaggctgg tcccctcagg
    14401 tagttttatt tcgatgttga actgatttga ttctgccccc ggcataaatt cactgccaag
    14461 ttttgggata agcagggcag ttaatcccat aattatgaag gcagaaatta aaacaatacc
    14521 tcttttttct acaatttttt cgagaatttt actgtaccat ccgaatttta cagaggctga
    14581 aatagctctg gcactctttt ctttaaaaat tgttgatacc agcatgggga taaccagaag
    14641 agcaacaaac agggatgaaa gcagggcaaa tgcaactgta agggcctgat ctttgaatag
    14701 ttcacctgat gctccatgta tgtaaacgat aggaagaaaa actactatcg ttgtgatggt
    14761 tgatgcagtg atagcaccgc ctacctgtcc ggtaccagtt attatagcat caatgaatgg
    14821 cattccttct tcccggtttc tgctaatatt ttcgagtaca acaatggcac tgtcaacgag
    14881 catgccggca ccaagggcaa gacctccaag agtcatcaca ttgatggtaa gatgattgaa
    14941 atacatcaga ttgaacgtcg caataataga tatgggtatg gcaagactta tcacaagtgt
    15001 tgccccgatc cggcgaagga agatgtaaat cacgaaaaca gccagcacaa tgccgatcag
    15061 agctgtattc tggacctcag ttattgcact gcgtatatat ttaccctgat cctgaataaa
    15121 aatgaattca tatcccggaa gagccctgga aagatttgca aattcctctt ttagtttttt
    15181 tactgcttca actgtattaa acttgggctc tttgtaaatg gagagtccaa tgcatcgttc
    15241 accattgatt gtgacaatat tatcaggttc cttgtttccg aaataaattt ttgctacatc
    15301 tttcaggtag attggcactc ttgatgaaga cgacgcagag gctgcaattc cggcagaagt
    15361 tgctgaagtt gtctgtttaa aaccaactat tatgttttca aggtctgtga ggtctctaag
    15421 aacactaaca cccttaacca cataccttgt ccccatttcc actattgagc cgcctgacac
    15481 atttctgttg tagttactta tctgggcagc tattgcatcg gatgttatac cataagattc
    15541 gagaatatgc ttttttgttt caatgatcac ttcactctgc tcgatgccag caagctttac
    15601 atctgcaacc ccgtctattc ttaccagttc atttcttatg tagttttctg caacttttct
    15661 gagttcatcc agattgttca aacgttcatt tttcagggcg actaccatga ctggggcagc
    15721 atttggatca tactgcgaca ttgtaaagtc ttcaatatct gaattctggg caaatgaatt
    15781 cagctctttc tgcaggtcga gaaaagcttc atccatatct tttttccagt cgtattctac
    15841 cgtaacaaca gcttttccca cttttgatga ggaagagacc tgaattaccc cgttctgccg
    15901 catggcaatc gattcgatct gatcgacgta atttttctct atctcttctg gtggcttttc
    15961 cccggctttg atttcgatat aaattttggg attattaagg tcgggaaaga ggtcggtgcc
    16021 cagcttgccg aaagaaataa agccaaggag cataactccc aggacaaaca tcagaacggt
    16081 aacagggtat ctgactgcaa aacgggctat tctgttcata ttttctattt aactattttc
    16141 actctggatc tgttactcag tgtttcgaag ccacttgtta ctattctttc gttcttcgac
    16201 aggcctctca caacttctgc ttctgaaagg ttttccagcc ctgtctcaat aattctttca
    16261 agggccagtc cccggtcaac gacaaatact gttttccctc gttgccgcga tagaattatg
    16321 cttttgggga tgacaatcac agagtctttc ctgttaataa ttatatcggc ctttacaaac
    16381 attccgggcc ggagaagtaa atcaggatta ttgactgtga taactccctg aaaagttctg
    16441 gtatcaggat ttattgcggg ggataattgt gttacttttc cataaagtgt atcttcagga
    16501 atagtgtaat tggtaagttt tacaggttgt tcaggtttaa ttacaccaat gtatttttca
    16561 ggtagctgga cattcatata catagtctgg taatccatga ttttagcaat tgttgatcca
    16621 gcttcaacct gagtcccggg cgtgtaataa gggagttcca cgattatacc atcaataggg
    16681 gagacaaccc tggttttttc aagttgcagc ctcgcatttt cgacagctgt ctttgcattt
    16741 tcatagttta tacccgccgt tttaaggtct tttaaagtca cacctccttt ttcgtaaagg
    16801 gattcctgct ttctaagttc gctttcagca agttcgagat tcagctcatt ggtttcgagt
    16861 tttatcgaca ggataaattc tttatcctcc agcctggcaa taagcgaacc tgctttaact
    16921 ctgtcgccaa gctgccatgg ccggccagtt tgcgggtttt tttcgaggta ataagaggcg
    16981 gaaatttttg ccttcagagt cacctctccg tgtggataga ctattccagt agtactgaca
    17041 tattcttcta ttgacttaag ccttatatct tcaaccgtaa caggaatttc aatgtcagct
    17101 gaaagatttg gctcctgatt tctacagcct gccagagaga ggattgcaaa agcaattaca
    17161 attaaaaata ttgacctttt catattaata ttattaaaat tttactggtt aagttttaag
    17221 accggcgaaa ccggttcttt cttttcaaaa tcgtataatg tctgtatttt aagatttagt
    17281 aattcaagtt tataggaaat tagggcatta gtataagcta actgccgttc tgacaactgg
    17341 ttctggtaaa tatttagatc cataccggtc agatcgccgt ttttgtatct ttcgaggttc
    17401 agttcatagg taagctgcgc atttgttact gatttccggg ctatctcaat ctgattctgg
    17461 aggttaatca gattcctgta tattttcctt atgccgagaa taatattatt ttgttcattt
    17521 tcgaaagata tttttgcact ttcaattgaa gcttcggtag ctttaatcct tgattttttt
    17581 tctccccagt cccagagagg aattgtaaga gataatgcaa ctgattcatt atctgtagga
    17641 tttgcataga cgtcactaaa tttttcgtta tcaccaaaaa gccctactga aaggctcaga
    17701 ttccctttga attcattaag tgcttttgtc tgaataagtt cgaactgtga gttttcaata
    17761 gatatctctc tctgacgaag ttccattctg tttttaagtc cgtgatcaat agcgaatgca
    17821 atgtcgacag gtattgtatc aactgatata tccggcaata caacgaaatc gtcatacagg
    17881 tccataccga taaggatttt gaaatcgtct tttgcatttt cgagagatac ctgtttgttt
    17941 tcaaaatccg attttgttgt tgcaaggtta agttctgcct gatatagttc gtcctgggct
    18001 gagaggcctg cctccacctt gtttttagaa atctcatagc tctgtttcat gttttcatat
    18061 gcttgttttg caatttccag actttgctgc tgctgataca cgtaataata tgcctgggca
    18121 acctgcctct caagtgaaag gagctgcagg gcatagttga gctgggcgtt ttcaagggca
    18181 agctgtaatt gtttaagttg aagctttgtc cggttatatg taaaaagagg ctgatcgaga
    18241 ttaattgaga ggttgttgct gaatccttta gtagtagcat tggtaaattc gctgtatgag
    18301 tctttgtagc caaatctgtt taccagtgat actcttgcat cagtaaacaa aatgggttgt
    18361 gatacagtaa acacactgta gctctcagtg gatcttctgg tattccattt tgatatcaga
    18421 tcgttgaatt ctctgctctg tgtgtatcct ataggattga ttgtgagtga gaagttcgat
    18481 tttagtgccg cattctgtgc attaaggttt tcctggcttc ttataaggct gagttttgtc
    18541 tgttttattg aggggctgtt agcttctgca attttcattg catcctgtaa ggtaagtcct
    18601 ttctgggcat gacagggaag ttccagggca gatatcatac agataaataa aagcagtggt
    18661 ttggtattta gcggttttgt tttcatatga gactttattt taaattttaa agtattttca
    18721 actagctacc tgttttaaat gtcattattt cttggttctc agcaccgatc tattccttca
    18781 gcacccattt tactgcatct gcaatcacaa ctctgccttc tgattggttt gtcatctcca
    18841 ctttaacggt atcgggtgaa aggtagaaag tgccaagttt gttccatccc ggatcagcgg
    18901 tttcgtaatc gatggttatt tcatccatac cgtcgtcgtg gtagactttg aaatggaagt
    18961 ctttgtacgg gttttgccgg gcgggttcgc cactctggtc acccgggggt ggtggtgtgc
    19021 ccggaggccc tccagctggt ggacgacctg gttgagcacc tgcctgcccc ggccttccag
    19081 ctcctctcat aataatctga gctccagcct tgccaatata tgcatataca tcataatagc
    19141 ctggttcttt tattatgccc ttccatgtca catatctgtc gcctgtgcct gctcttgtgt
    19201 aaacagcaga acgcacgaat cggccgtaaa aggtgttctg aactacaggc tgccagtatt
    19261 cgggtgcaac gaaaagattc attgattgat atgtctcagc gacttcctta tttccactca
    19321 atatttttct gagagggttc atcttagatg ttgcactcct gtaaaatcca gggtcttcat
    19381 tgtctactac aacttctccc ggttgtgtaa aagatggtat ttcctgaata atatcctctc
    19441 cttcaaaagg cttttgtttt gctgattctt ttttcacttc ctgaatggta aacaccagtt
    19501 ccccagggat atttttcgaa aacagggtat taatctgcat ggctctgggc tggctttcaa
    19561 gaaccactcc aatttttttc gtttcgtgag gtgagagata gacaattttt gatatgtcgg
    19621 aacttgcaag gcctcttccc tgcattgcta tttgcattat tctaccgcct cctgaggcac
    19681 ctcctcgtcc tccctgaaaa gttacagcca tagttgcctg tcctccgccc ctgcctccga
    19741 aaccgccacc tgtgcggaac gaaacattga aaagcccacc tgcagattca gtattggatg
    19801 ctgtaaaaac aacctgaaac cttgtccgct ggtcaaccac aatagttgtt acatcgggtt
    19861 ctgtaaaaat aaatccaggc tgctccctgc cattaaacca ttcttcgaga tatggataaa
    19921 attcaaatcc aaatctctcc tttaaatcat tattgaattt cagaatattt acccttttgt
    19981 agctgttgtc ccatatataa tttttaaacc attcgttaaa ttcgtttatg cctgccttat
    20041 atctcagata attaaacaga cagtctcctt ttagagtaag cacggcacgc atggtatcac
    20101 tgctgggatt tcgggccaga agctctttga agcttgtgtt tttgagaata agattagccc
    20161 tatcgttatc tgaaagatta ccggccatca ttcctggtcc tcctgcagga gcaggctcat
    20221 ttagcttctg gagatgcgat tcaaaaacag cattaataac aggatattca tctgagtaga
    20281 aattgtttct gaaaaagtaa aagcttggtc ctaacaaata ccttgttggt tcattgacaa
    20341 gcacaccatt taccattctg aaattacgcc cgcttataaa agtgtttctg atgaagttgt
    20401 taaagattct gactttcagc tctttatcgg ttattatctg attgttgcgt gccatctgct
    20461 ttttctgtct tgcaaacctt tttgcaaaac ctgcctgctg cagggtcgaa agcttttcag
    20521 ggataataac catcgagggt tgaatttcgg cccgggtctg tgtattgctt ttcggatacg
    20581 agtaaaactg aacaggagcc tccaccatgc ttaaacagga aaaaggataa gccatcccga
    20641 aacctgattc gagatctttc ataaaacctg agataagatt tggaagtgtg tctttaagtt
    20701 cttcaaaatc ttttttgtaa tagtcagtgc ccggataatg ccatgagatg aattttacgg
    20761 aatcgacagt tatactgtcg gcaacatagt ttcccattgc aacggtaagt cctgttagag
    20821 gaaacggaca tgagtatttt gtccattcac cttcttcccc gggtactccc tgtgaaacta
    20881 ccttaaggtt tttgtctgat tttgctttca gaatgaattt tgtgaaatcg actttaatcc
    20941 ttgccgggtt tgaagggtaa tagttcaaac ctgatacagg ataccagtgt gattccggag
    21001 taaagagcac atagttattt ttcagaaaag cttgcctttt attaacattt accatggcaa
    21061 ttctgtacct gttgtttttc ggatcatcac tgaaatttgg ataacaaaac gcttcagtaa
    21121 ttttgcccga ataaaaaatc gttacactat catatcctcc cggctcaagt ctctttgcag
    21181 gagaaatttc aattatatgt ccgtcagttt taaactgaat attgctgttt cctgaaatta
    21241 ctttttcaac tgataggcct ggattaagtg aaaaaatata tgattcaaga ggggtatcat
    21301 tatcatttac aattctgagc agggatacag cctcaattgt cttaccctta tgtgccagtt
    21361 cgatatctgc tgaagttgtt gaggggaatt tgcttttttc gtatttacgg ttgatttcaa
    21421 ttatgctttt tttaagcttt tctctttcca gatgattgtt taatgtgtta taaaatgtta
    21481 tgcctgaacc tataatacaa agtacgagta aggtccgggc taaattagta tggagtcttg
    21541 actgtggtag tcttttgaaa agaatcaccg aagccattat cattgatact cctgcaaaga
    21601 agtagcccag acgctggttg attatcgaaa atatattgtc gaatccaatt attcccgact
    21661 tgtaaagtgg cattccaaaa accatgtaat cgtataaaga tcctgccctg taccacagat
    21721 agaacatgtt aagtgctgca tatccgagca ggaggaaaaa tgtaattgcc tgattcccga
    21781 ttataagcat taatatatat gcaaggccca ggctgtaaat aagggtagga attgagataa
    21841 acaggaggta ttcaaaatat gaaagtatgt ctactttcat ggttttggaa ataatattta
    21901 taatcagtcc tattaataaa ataacaacat taagtgaaag gaaaaggcgc aatatgccta
    21961 aagtttttcc ggttacatat tcgaagttgg agacagatct ggtatataaa acttcgacag
    22021 tatcaagttt tttatccctt ttcagaaaat cggatgcaag aaatattaca acgactgcct
    22081 gaccaatgtt cagcaggtac agattgatca ggggtagggc tgaaggaatt gaaaccattt
    22141 cccatgattc agctcccacc ggagaaaaga ggcctatgtt caacattgta aaaatgaata
    22201 gcgcccctat ggcaaagagt ctgaagaacc aggttcttct cagggtcttt gcctcgtaat
    22261 gtgcaacagt gagaatatta tggaggaacg tcatttttta ttgaattaaa cgtttaaatc
    22321 ggcatgaatt ttatgttcca tataataaac gtatgcatgt tcaatattgg gttcaatttc
    22381 aactgcattc ccatcgggag ggagatcgga aacaatctgt acttcccatc cgttttcaac
    22441 tggtattgtg gagatgatgt catattcttc cttggctttc tcatactcat agggtgtgag
    22501 attcattttg aaaacatggc cttttgcttc atttaccagg ttttcgggac gtccacaaaa
    22561 gactacctcc cccttgttga gcaaagccat attctggcat gttgaggaga tatctcctac
    22621 aatatgtgtc gaaaggataa taattacatc ctgtttctga ctgagtgttg aaaggatgtt
    22681 tctgaatctt attctttcat caggatcaag acctgttgtt ggctcatcca cgataatgag
    22741 tttcggattg cctatcagtg cctgcgcaat accgagacgt cttttcattc ctcccgagag
    22801 cttgttggca ttcctgtcgc gtacctcgag taagcctacc tgatcaagca tacggtcgac
    22861 ttcttttaat ctttctttcc tgtttttcat tcctgcaagc gcagctgaat agtcgagaaa
    22921 ctcccatgtc tttaaagaag taaagaatct gaaatcctga ggaagataac caagaatttt
    22981 ccttacttcc tttctgtgct tctgaatgtc tttcccatcg agcagaacaa cacctgatgt
    23041 gggttttaca agagtaacaa gaattctcat cagagttgat ttacctgcac cgtttggacc
    23101 cagcagtccg aacatgccag attcaatcac cagatttatg tttttcaatg cgtgagttcc
    23161 gttcggatag attttattca aatcacggat ttctatcttc atataattct ttgatcttga
    23221 tgaaagttaa tcattttaat atttagacta ttaaaagaca tgatttattt cagattgtta
    23281 cacatttgat aaatgttttt aaacggtttt caatatttca tatctatcaa ttttacagat
    23341 agtagtgtat tgaagttatt tctatttttg tgctgtgaac ttttcccatg tggattacag
    23401 taca
//
LOCUS       DAOSNB010000009        21926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2373_length_21926_cov_4.909515, whole genome shotgun sequence.
ACCESSION   DAOSNB010000009 DAOSNB010000000
VERSION     DAOSNB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_21926_cov_4.909515"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLG42_00985"
     CDS             complement(<1..673)
                     /locus_tag="PLG42_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU29813.1"
                     /translation="MKLCAFRIKNFRSIVDTEWQNLSPDNITCLIGQNESGKTSVLEA
                     LRAFYTGVISEDVLRSDLSMPEITCRFSVKKGWLVEITENAGTELKELLSGISSLDLT
                     RTWMPDFTSVIKASGAISDYLDSLENAWKLYLEEVQEKLKAELETISNLENTIKEIVS
                     VKTDLSKRIGSSSPGGILTSLFHKEKSSGEGMSVSELKKQYSELVRDEERVRAELMKK
                     DKIKKA"
     gene            complement(685..1503)
                     /locus_tag="PLG42_00990"
     CDS             complement(685..1503)
                     /locus_tag="PLG42_00990"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU29814.1"
                     /translation="MFDLRSKQAEIISQAGLQALDEHLASLRYAGMIQHALMPDDELL
                     AGVLKDYFVMFLPRDIVSGDFYYAFPGKEYLCLAAGDCTGHGVPGALLSILGISFLNE
                     ILQSVRAVKANRVLNLMREKVMKALHQTGDRAETKDSIDIALCIVEYQTGKMQYAGAN
                     RPLVVVRNGHIIEYKPDFMTIGLAPVAERAFKNNYIDIFPDDQFYLFSDGFPDQFGEK
                     TNKKLKNKNFRKILEIASGIPEMSAQKAYLEKKYYEWKGNMTQIDDVLVFGFKL"
     gene            complement(1642..3048)
                     /gene="rlmD"
                     /locus_tag="PLG42_00995"
     CDS             complement(1642..3048)
                     /gene="rlmD"
                     /locus_tag="PLG42_00995"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOU29815.1"
                     /translation="MGGGNYLSVYEKVEVTDIGAGGIAVAKAGSLVIFLPGAVPGDVA
                     DIQIVRRKRNYLEGVPVKFHKMSPDRKPPLCEHFGVCGGCSWQCLKYEKQLFFKEKSV
                     KDAMERIAKAEIRESHQILGSPQQYYYRNKLEYTFSEKKWLTDEEIKTTQTLSRENAL
                     GFHKPGHFDRVIDIYKCHLQPEPTNLIRNALRDYAKTLGLSFYNLKEHRGFLRNLIIR
                     NTLGGRVMVIVVFHEQEEEKVKGVLEFLRKEFPDIASLMYIINKKKNDTITDQEVLLH
                     SGDAYLTEEMNDLKFRIGPKSFYQTNTRQAIRLYETVKQMAALTGKEIVYDLYSGTGT
                     IACFLARSSRSVTGLEYVREAIDDARINAEINNLGNVTFIEGDIKELLSEGLFGKMGR
                     PDVVITDPPRSGMHKDVVMAILKAAPERIVYVSCNPATQARDIQLLSEKYSVDSIQPV
                     DMFPHTQHVENVALLIRK"
     gene            complement(3068..4297)
                     /locus_tag="PLG42_01000"
     CDS             complement(3068..4297)
                     /locus_tag="PLG42_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOU29816.1"
                     /translation="MNRKLWFTGFQILLFLFLVSSCKKEEESPYSYLVSTEKATSYTT
                     QYINTLLDNAVSYYPEISAVKPFISVGVDVYIIFYTTDIGTKKIKASGLMCVPQLPGS
                     YPVISFQNGTNTVDAYAPSNFFVNPQYQMIEIIASMGYIVLLPDYPGFGASASVAHPY
                     LVKEPTVRSIVDMLRAVKEAAGNEIRNIEVKNEYYFIGYSQGGWATMAMHNAMENEYG
                     NEFNLAGSVCGAGPYDLYFLLSNMTDVTVYPMPVYICYIINAYKAYNQFTNPVTDILK
                     EPYASKLPLLFNGKQDFGQINSQLTTSVPDLLTAEFLAGFKSDSKYEPVRNALVSNSI
                     QAWKTKKPLYMLHGGSDTQVNPQVTEYFYSAMISAGSSTETVKKEILEGLDHGDAAAP
                     AIIKGLLFINDIHSLLN"
     gene            4541..6484
                     /locus_tag="PLG42_01005"
     CDS             4541..6484
                     /locus_tag="PLG42_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOU29817.1"
                     /translation="MLSVENISVSFGSFELLKDISFLVNKNDRIGLAGKNGAGKSTLL
                     RIIAGLQSPTTGRVNKPKNFRIGYLPQQMSTNITSTVIDETLKAFDELFEISANISLI
                     TKNISSRTDYHSAEYMDLCDSLAVLEEHYRMLGGNEYMAQTEKTLVGLGFDRENFDRP
                     TGELSEGWRMRIELAKIILRKPDLMLLDEPTNHLDIESIQWLESYLAEYPGAVIVVSH
                     DRAFLDNVTKRTIEISLGKIYDYRVPWSEFVELRKERREHQLAAFRNQQKMIADTEKF
                     IERFRYKATKAVQVQSRIKQLNKLERIEVDEEDTSTINIRFAPAPRSGTVVIEAEGLA
                     KSYGDHPVLDNISLTVTRGEKVAFVGRNGEGKTTLSRIIAGELDYKGKLKIGHNVKIG
                     YYAQNQDEIINEDKTVFQTVDEIARGEVRTRLRDILGAFLFRGEEIDKKVKVLSGGER
                     SRLALVKLLLEPYNLLLLDEPTNHLDMQSKEILKQALLKYDGTLIVVSHDRDFLDGLV
                     DKVYEFRNRKIKEHTGGIYDFLRMKKIESLRELETPEKQAVEQKKEEDISLNKQRYLE
                     KKEFERNLRKLRKRVEDIENEIEEIENKIAEMDTKLSEPGNEALFSEEFFSRYNELKE
                     KLSNKIALWEQYNIELDSFLKSN"
     gene            6487..7242
                     /locus_tag="PLG42_01010"
     CDS             6487..7242
                     /locus_tag="PLG42_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOU29818.1"
                     /translation="MQEAIYRKEYSVHVYELGIDEKLSPFALFNYFQDIAAEHAVNLG
                     FGREDLLKMNCFWVLSRMAVVVKNWPLWKDRLVITTWPRGTDRFFAIRDFKVEYPGGN
                     EVAAATSSWLIVDINTRRIRKPDENLLKNRPGLADLSATGAYAGKIEPLKEFTDISGS
                     FMVKTSDLDVHRHTNNAVYIKWVTDAYSHDFREKYFPCSFEVNYLAESKQGDEIIIKT
                     FTEDNLQFYHSVFRTADNTELCRIRIRWRNCMH"
     gene            7264..8595
                     /locus_tag="PLG42_01015"
     CDS             7264..8595
                     /locus_tag="PLG42_01015"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOU29819.1"
                     /translation="MKYSKITYFLSGVLLLAAIAVSCVSTRPVYDPKDLSYLYNPLKT
                     RINPRYSVFNESDNRSLLGVKFFTGDLFFSEANPAGVPMARMYIYARLYNTTFGRVIT
                     DTAYYDIDIVKDPNKEEYLYKIPLKVQKGMEYVCEVKIYDKLRDFMIQAFVPFNTTSD
                     FNRYNFYVRGHLLKNELLKPVVRKDEYFNIFYGRGRPDSVFVSVYPLSEDIPYPPSMV
                     LPERSVPVKPDTVIAIPYSDTLPLMLPEKGIYFFTVSREIREGITLFNFGDDFPTMTT
                     PEEMIKPLAYLLPEDELQKIASSEKPKVALDDFWLGCCNGNVEKARELIRIFYTRAVF
                     ANYYFTSWKEGWRTDRGMIYIIYGPPDKLYKSSDVETWGYRKPVVSSGWGARVSVKEE
                     YLFFTFKKKDNPFTDNDYSLSRSETVVSYWDKAVQSWRKGIVFRLDNPEEI"
     gene            8599..9363
                     /gene="rlmB"
                     /locus_tag="PLG42_01020"
     CDS             8599..9363
                     /gene="rlmB"
                     /locus_tag="PLG42_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOU29820.1"
                     /translation="MSGETEFIFGLRPVIEAIKEGKQIERLLIRQGLQGQLYHELMKE
                     VREHSIPYQIVPVEKIEQHTRKNHQGVLAFMSLIEYQDISNILPAIFEKGEDPLILCL
                     DGITDVRNFGAIVRSAECFGAHAVLIPERGSARITADAVKTSAGALNSFPVCRTRSLT
                     KTIQFLKESGLKVLCADEKSNKRVFNATLTGPLVLVMGSEDRGISRELSALADETVRI
                     PLKGKTGYLNVSVAAGVILYEITRQRASSDGNLKNF"
     gene            complement(9334..9618)
                     /locus_tag="PLG42_01025"
     CDS             complement(9334..9618)
                     /locus_tag="PLG42_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOU29821.1"
                     /translation="MAKGEPLRKKFKGDASEKCTLILYNDDVNTFSHVIKSLVEVCGH
                     DEIQAEQCAVLVHFKGKLEVKSGSPEMIMAMSEKLNKLGLKSKVLKIPVR"
     gene            complement(9620..11149)
                     /locus_tag="PLG42_01030"
     CDS             complement(9620..11149)
                     /locus_tag="PLG42_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29822.1"
                     /translation="MKRWFFTWSILFVIYCAADVTAQEVQQRATRQTAAEAYNKGLYE
                     DALRQFSELLALYPRDPLYKYYCGVCLVKLESEPVRAVKLLKEAAEGSGTIKPVPADV
                     WFWLGRAQQLAGMFSEAVFSYNRFTESAGRKVAREMDVPDYIKQCNEGKGSIAEEVQA
                     AVKKTEQVKISQEKPEGKPPAGGGVERQAAEAEAMYAPVEVDTMLARLLEEKKQMPEI
                     IGKEAGNKDTVIKQKTVTEPGPEVENNKIQAAAKLPAQEVKAPDKKVEVEPVIKNLPA
                     ESREPVYSQFEILKKPVYAPDDKVPINPDVPPGLIYRIQIAIFRNPVAPSYFKGLTPV
                     HGFRREESNLTVYYAGMFRKAEDAYVALQKVRGLGFKDAFVVALMDKQQISMERAKVL
                     EKEWGSKPLFEITPIVAEKDTVPPTLVFRVEVMKTPKPLTGQQLEELKKLATGKGFDV
                     LVDDKKNNIYLIGIFINYTTAVEYADLLKRNGYRDARVVAYLGLKEIPVDIARKLFEE
                     Y"
     gene            11325..11783
                     /gene="bcp"
                     /locus_tag="PLG42_01035"
     CDS             11325..11783
                     /gene="bcp"
                     /locus_tag="PLG42_01035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOU29823.1"
                     /translation="MADLKEGMKAPEFEGKDQNGNIIKLSDFKGKKLVLYFYPKDNTP
                     GCTAEACNLRDNYNVLINKGYAILGVSPDPVKSHKNFSEKYSLPFPLIADTEKEILKL
                     YGAYGEKKMYGKTVTGVIRTTFVINEEGIIEKIIRKVDTKNHAEQILDKN"
     gene            11812..12822
                     /gene="recA"
                     /locus_tag="PLG42_01040"
     CDS             11812..12822
                     /gene="recA"
                     /locus_tag="PLG42_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOU29824.1"
                     /translation="MSSEKKEINKEKLKALEVTIGKIEKDYGKGTIMKLGDHPVDSVQ
                     VISTGSIGLDAALGVWGLPRGRVIEIFGPEASGKTTLAIHAIAEAQKNGGIAAIIDAE
                     HAFDRNYAEKLGVDVENLLISQPDNGEQALEIADHLIRSGALDIIVIDSVAALTPKAE
                     IEGEMGDSKMGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPETTTGG
                     NALKFYASVRLDIRKINQIKEGEEIIGSRTRVKVVKNKLAPPFKKADFDILYGEGISQ
                     AGEIVDLGVDFDIIKKSGSWFSYGDTKLGQGRDAVIKLIKDNPELYEELKAKVIEALK
                     KQ"
     gene            12845..14905
                     /locus_tag="PLG42_01045"
     CDS             12845..14905
                     /locus_tag="PLG42_01045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU29825.1"
                     /translation="MKKLFVLMLAAGMVFVFSCKQKPAKEAELKPPFSVELADSEKAS
                     GLMTPEIMWKFSRLGSFALSPDGSEVVYSITRIDLATEARITNLYKIPTTGGEPVQLT
                     YEGGSSPQWFNNGKKIAWVSEGNLMTMNPDGSGKQKVEGLSDFEIYRISPAGNKIFFT
                     RRVKLDQTANEKHNLPKAKVRIIDDLMYRHWNYWHDYSYSHLFIASFDGVKVSDEKDI
                     MEGQKFESPLSPNFDENEITWSPDGRYIAYTTKRLRGKEDAVSTNSDIYLYDVTTGKE
                     VNISKGNMGYDRYPVFSPDGSMIAWQSMERDGYEADLDRLFVYNIKDSTRTWVTKGWD
                     FDVEDITWADNNTIYFTCSYLGTKQVFRTDLMSRKVIKVTEGMHDLGPLRLQSGTMIA
                     QIMSMSMAPEIASIDLKTGAVNKITKINDYIYDHIRMGKVEEKYTKTKDNKDLQMWVI
                     YPPDFDPSKKYPLLLFCKGGPQGPMSQWWSYRWNFQMMAAKGYIIVAPNRRGSSGFGQ
                     AWKEQISGDYGGKNMQDYLDATDAMAKEPYVDANRMGAVGASYGGYSVFYLAGIHQKR
                     FKAFVAHCGMFNIESWYGSTEELWFPNMDIGGPYWENHPGYKFSPHLMVKKWDTPILI
                     ITGQNDFRIPYTQSLEAFTAAQLLGVPSRLLFFENEDHFVSKPQNAVIWQREFFAWLD
                     EWLK"
     gene            complement(14999..15916)
                     /gene="miaA"
                     /locus_tag="PLG42_01050"
     CDS             complement(14999..15916)
                     /gene="miaA"
                     /locus_tag="PLG42_01050"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU29826.1"
                     /translation="MTEYDLVIITGPTASGKTAFAAALAARIEGEVISADSRQVYRRM
                     NLGTGKDYSDYVVDGRQIPVHLIDIVEPGYKYNVFEFQRDFLRVYEDIKSRGKFPVVC
                     GGSGMYIDSIVSGYKLAEVPPNPELRKELENKTLDELTEILKQYKRLHNKTDIDTVKR
                     AVRAIEIEKYYEAHRDIIRDFPKLKSLVTGVFFDRETRRKRISERLKQRMDAGMVKEV
                     QELLESGIDSDTLIYYGLEYKYITLYLNGQLSYDEMYRQLEIAIHQFAKRQMTWFRGM
                     ERKGVKIHWIDGNLPQEAKIEKIMELLTT"
     gene            16001..16477
                     /gene="smpB"
                     /locus_tag="PLG42_01055"
     CDS             16001..16477
                     /gene="smpB"
                     /locus_tag="PLG42_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOU29827.1"
                     /translation="MKAGRENIVIRNKKATFDYELEEKLIAGIVLTGTEIKSIRAGKA
                     ALTDSYCHFVDGELYIKGMHIAEYDYGNIRNHDPLRERKLLLTKRELRRLEKKVREAG
                     YTLIVTKIFINERGLAKAELALAKGKKMYDKRESIKRKDAAREMERALKGRGGKSY"
     gene            complement(16474..17223)
                     /locus_tag="PLG42_01060"
     CDS             complement(16474..17223)
                     /locus_tag="PLG42_01060"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU29828.1"
                     /translation="MKIVCKTDTVWIAGQYNHVSVDVTFDASDGLVRFTQDFPEGVEV
                     APENIPAGDFSWYNNQLNIVWLTIPASKKISFSYLVMPGKSMSGSFDLEGRLVMITGG
                     KTKRTFSMNNIKVSIKGAQTSEAVNTEAGKRNVMQDGRGDVVSEPRGRQAAPSDRNIE
                     FRVQVASYSTPLPAEQVAKKLGLWPGEKLVMVKVGNVYKYQVGSFSVYEEAVALQKRL
                     AASGLKDAFVVAYSGETQIPVSEAIRIIKSR"
     gene            complement(17294..18004)
                     /locus_tag="PLG42_01065"
     CDS             complement(17294..18004)
                     /locus_tag="PLG42_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU29829.1"
                     /translation="MEKPADLIKDAFEQKTWQEIHITDSWRVFKILSEFVEGFEKLAR
                     IGPCVSIFGSARTKPDNKYYKMAEEIAFKLTQKGYGVITGGGPGIMEAANKGAWRGKG
                     KSVGINIDLPFEQKPNPYIDSDKLITFDHFFVRKVMFVKYAQGFIVMPGGFGTFDELF
                     EAMTLIQTRKIGKFPIILVGKKYWEGLIKWIKEVMLEQEHNISPQDLDLFTVVDTADE
                     AVDLIDKFYSRYLLSPNF"
     gene            complement(18095..20926)
                     /gene="uvrA"
                     /locus_tag="PLG42_01070"
     CDS             complement(18095..20926)
                     /gene="uvrA"
                     /locus_tag="PLG42_01070"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOU29830.1"
                     /translation="MSDRLTVLGARVHNLKNIDVTIPRNKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYMETLSAYARQFLGGLERPDVDKITGLSPVISIEQKSTSKNPRSTVGTI
                     TEIYDYLRLLYARAADAYSYASGEKMVRYNDEQIIELIEKGFHGKKINILAPVVRGRK
                     GHYRELFERLRLKGYLYARINGEIREITPGMKLDRYKTHFIEIVIDKFRVGEVSHKRL
                     RESVLLALDQGDGVMMILDRESDKIRYFSRHLMCPVTGISYNEPAPHTFSFNSPQGAC
                     PHCNGLGEISDIDINKIIPDRSLSIRKGGIEPLGKYRNTWIFWQIEAILEKSGYTIDT
                     PISELPEDVVNKILFGSDEVLILSNTPLGLTSNYFLTFEGVISFLGNLEVINGQRNGS
                     RLKDQYVEYRTCPECNGNRLKKEALWFRIADKNIGELAAMDIRELYSFLASVEEKMSE
                     KQKKIASEILKEIRARLSFLLEVGLDYLSLNRSARTLSGGESQRIRLATQIGSKLVNV
                     LYILDEPSIGLHQRDNRRLISSLRKLRDEGNSVIVVEHDRDMIMSADHIIDVGPGAGV
                     HGGRIVAQGPPQEFMHCETLTAAYLSNCKRIEVPDERRRGNGKYLIIRGASGNNLKNI
                     DVAFPLGMFICVTGVSGSGKSSLINHTLHPALARKYHNSGKTPLPYHDIIGTEHIDKV
                     IEVDQAPIGKTPRSNPATYTGVFTDIRSLFAQTAEAKIRGFGPGRFSFNVKGGRCEGC
                     EGAGVKTIEMNFLPDVYVLCTECNGKRYNRETLEVKYKGKSISDVLEMTVDEAVEFFS
                     NVPAIYHKIKTLQDVGLGYIKIGQQSTTLSGGESQRVKLATELARRDTGKTLYILDEP
                     TTGLHFEDVRVLLNVLNRLVDRGNTVIVIEHNMEVIKMADYIIDLGPEGGKEGGYVVF
                     SGPPEEIIKCEKSYTGAFLKRELEKTVHK"
     gene            complement(20952..21902)
                     /locus_tag="PLG42_01075"
     CDS             complement(20952..21902)
                     /locus_tag="PLG42_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU29831.1"
                     /translation="MRYKPPRNIYYLLLPALFIGAVFLLSGFFNDSRPEKLPVNGKSP
                     FMLDIKLPDTIYFAGERVPLENFDTRESLEREIIMTAYRHGSTIMIIKRAPRYFPVIE
                     KILSENGIPDDFKYVAVAESELSNMVSPAGAVGFWQILAGTGREHGLEINNEIDERYH
                     LEKSTVVACDYFKKSYEKYGNWTLAAASYNGGRNAIDEQISIQKQTNYYDLLLAEETA
                     RYIFRILSYKIIISNPAAYGFEIDKEELYPPLRYTEVKVDTAVADFARFAAHFGTNYK
                     ILKFYNPWLRKPYLSKKSGKSYYIKIPEEGSRTTLYENEI"
BASE COUNT         6325 a         4867 c         4434 g         6300 t
ORIGIN      
        1 cggctttctt tatcttatcc tttttcatca attctgcacg aacacgctcc tcatccctga
       61 ccagttcact gtactgtttt tttaattcgg aaacgctcat tccttcacca ctggactttt
      121 ccttatgaaa gagacttgta agaatgcctc caggtgaact ggaaccaatt ctttttgaca
      181 aatctgtttt aacggaaact atttccttaa tagtattttc aagattgctt atggtttcca
      241 gttcggcttt aagtttctcc tgaacctctt caagatagag tttccaggca ttttccagtg
      301 aatcgaggta atcgcttata gcaccgcttg cctttattac cgaagtaaaa tcgggcatcc
      361 atgtacgtgt gagatcaagc gaactgattc cggaaagaag ctctttaagc tcagtcccgg
      421 cattttcagt aatctcgacc agccatccct tcttaacaga aaacctgcag gtaatctccg
      481 gcattgaaag gtcgcttctc agcacatctt cagaaatgac cccagtataa aaggccctca
      541 gagcctccag tactgatgtc ttccccgatt cgttctgccc tatcagacag gtaatattat
      601 ccggagacag attctgccat tctgtgtcaa ctatcgaacg aaaattcttt atcctgaatg
      661 cacaaagctt catgaaaaac ttatttacag tttaaatccg aataccagaa catcgtctat
      721 ctgtgtcata ttccctttcc actcgtaata ttttttttcc agatatgcct tctgcgccga
      781 catctccgga atgcctgacg caatttcgag aatttttctg aaatttttat tcttcagctt
      841 tttattcgtc ttttccccga actggtcggg aaaaccatcg gaaaacaaat agaattgatc
      901 atccggaaat atatcgatat aattattctt gaaagccctt tcagcaacag gtgcaagccc
      961 gattgtcatg aaatcaggtt tgtattcgat tatatgcccg ttacgcacaa caacaagagg
     1021 ccggtttgcc cctgcatatt gcatcttacc cgtctgatat tcaacaatac acagtgcaat
     1081 atcaatgctg tcttttgtct ctgccctgtc gcctgtctga tgcaaggcct tcatcacttt
     1141 ctccctcatc agattcaata ccctgttggc ttttactgct cttaccgatt gaagtatttc
     1201 gttaaggaaa cttattccca gaatactcag cagggcacca ggaacaccat gtccggtaca
     1261 gtcacccgct gccagacaca aatattcttt cccgggaaaa gcatagtaaa aatctccgct
     1321 tacaatatca cggggaagaa acattacgaa ataatctttc agaactccag caagcagttc
     1381 atcatcaggc atcagtgcat gttgaatcat gcctgcatat ctcagacttg ccaggtgttc
     1441 atcaagtgcc tgaagacctg cctgggaaat aatttctgcc tgctttgacc ttaaatcaaa
     1501 catcccttca aaaacaaaaa gttaaattta cattattatt ctttaataac tataaatcag
     1561 tgtaaacgta ttgttatttt cttgcattta cccactcacc ttttcacctc tttaataaaa
     1621 tcatatagaa aaactttctt actactttct tataagcagt gcaacattct ccacatgctg
     1681 tgtgtgtgga aacatgtcga ccggctggat agagtccacc gaatatttct ccgatagaag
     1741 ctgaatatcc cttgcctggg ttgcaggatt gcagcttacg tacacaatcc tttcaggtgc
     1801 agctttgaga attgccatca caacatcttt atgcattccc gaccttggag gatcagtaat
     1861 aacaacatcc ggcctgccca ttttgccaaa taatccttcc gaaagtaatt ctttaatatc
     1921 cccctcaata aatgtcacat ttcctagatt atttatttca gcatttattc tggcatcatc
     1981 aatggcttcc ctcacgtatt caaggcctgt cacactgcga gaagatcgtg caagaaaaca
     2041 tgcaattgtc cccgtacctg agtacaggtc gtaaacaatc tcctttccgg taagcgcggc
     2101 catctgcttg acagtctcat acaacctgat ggcttgcctt gtattagtct gataaaatga
     2161 tttcggacct atcctgaact ttaaatcatt catctcttcg gtaagatagg catcaccact
     2221 atgcagcagc acctcctgat cagttattgt gtcatttttc tttttgttta ttatgtacat
     2281 cagggatgca atatcaggga actcttttct gagaaactca agcacacctt taactttttc
     2341 ctcttcttgt tcatgaaaaa caacaattac catcactctg cctccgagtg tatttcttat
     2401 aatcagattt ctgagaaagc ctctgtgttc cttcaggtta tagaatgata gtccaagtgt
     2461 ttttgcataa tctctcagag catttctaat cagatttgtt ggttccggct gaaggtggca
     2521 tttgtaaata tcaatcactc tgtcaaaatg tccgggttta tgaaaaccaa gtgcattttc
     2581 tcgactaagg gtttgtgtgg tttttatttc ttcatcagta agccattttt tttccgagaa
     2641 tgtatattcc agcttgtttc tgtaatagta ctgctgaggc gagccaagta tctgatgcga
     2701 ttccctgatt tcagcttttg ctattctttc catcgcatct ttaactgatt tctccttgaa
     2761 gaacaactgt ttttcatatt tcaggcactg ccatgaacat cccccgcata cgccgaaatg
     2821 ttcgcacaga gggggctttc tgtcgggtga cattttatgg aatttaaccg gaaccccttc
     2881 gagataattt ctcttcctgc ggacaatctg tatgtcggcg acatcaccgg gtactgcccc
     2941 tggaagaaaa atcaccaggc tgccggcttt tgccacagca ataccgccgg ctcctatatc
     3001 tgtaacctca actttttcgt aaacagaaag atagtttccc cctcccactg caaaaagact
     3061 atttgtttca gtttaagagc gaatgtatat cattaataaa aaggagtccc ttgattattg
     3121 ccggtgcagc ggcatcacca tggtcgagcc cctcaagtat ttctttcttg acagtttcag
     3181 tagaactgcc ggcagaaatc attgctgaat aaaaatattc tgtcacctgc gggtttacct
     3241 gagtatcgct gcccccatga agcatatata gaggcttctt ggttttccat gcctgtatac
     3301 tgttcgaaac cagagcattt ctgaccggtt catacttcga atcactctta aatcctgcaa
     3361 gaaactctgc tgttaaaaga tccggaacag aggtggtaag ctgtgaattt atctgtccga
     3421 agtcctgctt gccgttgaaa agtaacggta attttgaagc atatggctct ttcaggatgt
     3481 cagtaaccgg attggtaaac tgattatatg ccttataggc attaatgatg taacagatat
     3541 aaacaggcat cggataaact gtcacgtctg tcatgttcga taaaaggaaa tagaggtcat
     3601 atggcccggc accgcatacg cttccagcca gattgaactc attcccatac tcattttcca
     3661 ttgcattatg catcgccatt gttgcccatc ccccctgtga atatccgata aaataatatt
     3721 cattttttac ttcaatgttt cttatttcat ttcctgctgc ttccttgaca gcacgaagca
     3781 tatcgacaat cgacctcact gtaggttctt ttacaagata aggatgcgcg actgaagccg
     3841 aagcaccaaa tccgggataa tctggtaaaa gaactatata ccccatggat gcaatgatct
     3901 caatcatctg atactgcgga tttacgaaga agttcgaagg tgcataggca tcaacagtgt
     3961 tagttccgtt ctggaagcta atcacaggat aacttccagg caactggggc acacacataa
     4021 gcccggaagc ttttattttc tttgtaccga tatcagttgt gtaaaaaatt atataaacat
     4081 caacaccaac tgaaataaaa ggcttaaccg cacttatttc aggataatat gataccgcgt
     4141 tatccagtag tgtattgata tactgcgtgg tataactcgt ggctttttca gtggaaacaa
     4201 gataactgta aggagattct tcttcctttt tacacgatga aaccagaaaa aggaaaagca
     4261 gaatctgaaa tccggtaaac cacaatttcc tgttcatatt attttgctga ttatcaattt
     4321 gttataaaat attgtttacc aacaccttac atcactccct gataaattaa taatgagata
     4381 tttacaattc tcttatttac tcataaaatt aaaacaagaa cttgataatt aattattaaa
     4441 ccttttattt ctaattaaaa accaggagcc tctgttaatt attaacccgg tagcaaagtt
     4501 acacaaagca tttcatatat ttgccctttt caatcaggtg atgttgtcgg ttgaaaacat
     4561 atcggtctct ttcggcagtt tcgaattgct taaggatatt tcttttctgg taaacaaaaa
     4621 tgaccgtata gggcttgcag ggaaaaatgg tgcgggaaaa tctactttgc tgagaattat
     4681 tgcaggtctt cagagtccga ctaccggaag ggtaaataaa cctaaaaact tccgtatcgg
     4741 ttatctcccc cagcaaatgt caacaaacat cacctccacg gtgattgatg aaacacttaa
     4801 agctttcgat gaattatttg aaatatcggc aaatattagt ctgataacca agaatatatc
     4861 atcacgtacc gattaccatt cagctgaata tatggatctt tgcgacagtc ttgcggttct
     4921 tgaagagcat tatcgtatgc tgggaggtaa tgaatatatg gcacagacgg aaaagacact
     4981 ggttggtctg ggattcgaca gggaaaattt tgacagaccc accggcgagc tcagtgaggg
     5041 ctggaggatg agaattgagc ttgcaaagat cattctgcgc aagcctgatc ttatgctgct
     5101 tgatgagccc accaatcatc tcgacattga atccatccag tggcttgaat catatctggc
     5161 agaataccct ggtgccgtta tagtagtttc tcacgaccgg gcttttctcg ataatgtaac
     5221 taaacggaca atagaaattt cgctgggaaa aatatatgac tacagggttc cgtggtcgga
     5281 atttgtagaa ttaaggaagg agagaaggga gcatcaactg gcagcattca ggaatcagca
     5341 gaaaatgata gccgacaccg aaaaattcat cgagcggttc agatacaaag ctacaaaggc
     5401 agttcaggtg cagtcgagga taaaacaact gaacaagctc gaaagaatcg aagttgatga
     5461 ggaagatact tctacgataa atataaggtt tgcacctgcc ccgcgttcag gtacggttgt
     5521 gattgaggcc gaaggcctgg caaaatcgta tggtgaccat ccggtactcg acaatatttc
     5581 acttaccgta acccgtggcg agaaggtcgc atttgtaggc cgcaatggcg aaggaaaaac
     5641 aacattgtcg cgcatcatag caggagaatt agattacaaa ggcaaattga aaatcggaca
     5701 caacgtaaaa atcggttatt atgcccagaa ccaggatgag attatcaatg aagacaaaac
     5761 tgttttccag accgttgatg agattgccag gggtgaagtg agaacaagac tcagagatat
     5821 tcttggtgca tttcttttca ggggcgaaga aattgacaaa aaagtaaagg tgctctctgg
     5881 tggtgagagg tcgagacttg cactggtcaa actgctactt gaaccttata acctgctcct
     5941 tctggatgaa ccaacaaatc acctcgacat gcagtcgaaa gagattctta aacaggcttt
     6001 gctgaaatac gatggcacat taattgttgt ttcacacgac agagattttc tcgatggact
     6061 tgtggataaa gtttatgagt tcagaaatag aaagataaaa gaacacacag gaggaattta
     6121 tgactttctg aggatgaaaa aaatagagag cctgagggag cttgagactc cagaaaaaca
     6181 ggcagttgag caaaagaaag aagaggatat ttcattgaac aagcagcgtt acctcgaaaa
     6241 gaaggagttt gagcggaacc tcagaaagtt gagaaaacgt gtggaagata ttgaaaatga
     6301 aatcgaagag attgaaaaca agattgctga aatggacact aaactctctg aaccgggtaa
     6361 tgaggcactc ttttcagagg agttcttcag cagatataat gaactcaaag aaaaactttc
     6421 aaataaaatt gcgttatggg aacaatataa tattgaactc gacagttttc tgaaaagcaa
     6481 ctgattatgc aggaagcaat ataccgcaag gaatacagtg tacatgttta tgaattaggc
     6541 atcgacgaaa aacttagtcc tttcgccctt ttcaactatt ttcaggatat tgctgcagaa
     6601 catgcggtaa atcttggttt cggaagggaa gaccttctaa aaatgaactg tttctgggta
     6661 ctttcccgca tggctgttgt tgtaaagaat tggcctctgt ggaaggaccg tctggttatt
     6721 accacctggc caagggggac tgacaggttc tttgccataa gagatttcaa agttgaatat
     6781 ccgggaggaa atgaggtggc tgcagcaaca tcatcgtggc taattgtgga cataaacaca
     6841 cggcgcatac gtaaacccga tgaaaatttg ctaaagaaca gacctggtct tgctgacctg
     6901 tctgcaacag gagcatatgc cggaaaaata gaaccattaa aggaatttac agatatctca
     6961 ggttctttca tggtaaaaac cagtgatctt gacgtccacc gtcacacaaa caatgccgta
     7021 tatattaaat gggtgactga tgcctacagc cacgacttca gggaaaaata ttttccatgc
     7081 agttttgaag taaactacct tgccgaatca aaacaggggg atgaaattat tattaaaacc
     7141 tttacggaag acaacctgca gttttaccat tcagttttcc gtactgctga taatactgaa
     7201 ctctgtcgaa tccgaatcag gtggagaaat tgcatgcatt gaaaagttta ttacatttaa
     7261 aacatgaaat acagtaaaat aacatatttt ttatccggtg ttctgttact tgcagccatc
     7321 gctgtctcat gcgtttcgac ccgtccggta tatgatccca aggatctttc atacctgtat
     7381 aacccgctga aaacaagaat taatccgagg tacagcgtat tcaacgaatc ggataaccgt
     7441 tcactgctcg gtgtaaaatt ttttacagga gaccttttct tttctgaagc caatcctgca
     7501 ggagttccga tggcgaggat gtatatttac gcaagactct acaacacaac tttcggaaga
     7561 gtcattaccg atactgcata ttatgatatc gacattgtaa aggatccgaa caaggaagaa
     7621 tacctttaca aaatacctct gaaggtccag aaaggaatgg agtatgtatg tgaggtaaaa
     7681 atatatgaca agctgcgtga tttcatgata caggcatttg ttccgttcaa caccacctct
     7741 gattttaaca ggtataattt ctacgtaaga ggacaccttc taaaaaacga acttctcaag
     7801 cctgttgtaa gaaaggatga atatttcaat atcttttacg gaagaggcag gcctgattcg
     7861 gtttttgtct ctgtttaccc tctttcggaa gacattccct acccaccttc tatggtactt
     7921 cccgaaaggt cggttccggt aaaacccgac actgtgattg ccatccctta ttctgatact
     7981 ttaccgttga tgctacctga aaagggaata tatttcttca ctgtgtcgag ggaaataagg
     8041 gaaggcataa cgctcttcaa ctttggcgac gactttccca cgatgaccac acctgaagaa
     8101 atgataaagc ctctagcata cctgttacct gaagacgaat tgcagaaaat tgcttcatcg
     8161 gaaaaaccca aagttgcact tgatgatttc tggctcggct gctgcaatgg aaatgttgaa
     8221 aaagccagag aattaatacg aatattttat actagggctg tattcgcaaa ctattatttc
     8281 acttcctgga aagaaggctg gagaaccgac cgtggcatga tatatattat atatggacct
     8341 cccgacaaac tctataaatc gagtgatgtg gaaacatggg gttatcgcaa acctgtagtc
     8401 agttcgggat ggggtgcaag agtatcggtt aaagaagaat atctcttttt tactttcaaa
     8461 aagaaagata atccgtttac agataacgat tattcattga gcaggagtga aactgttgta
     8521 agttactggg acaaggcggt acaatcgtgg cgtaaaggga ttgtattcag gctcgataat
     8581 ccggaagaga tctgaaaaat gagcggcgaa acagaattta tttttggact cagacctgta
     8641 attgaggcca taaaggaagg caagcaaata gagcgccttc tgataaggca gggcctgcag
     8701 ggacaactat accacgaact aatgaaagaa gtaagggagc attcaatacc ttaccagatt
     8761 gttcctgttg aaaaaattga acagcataca agaaaaaacc accagggtgt gctggcattc
     8821 atgtcattaa ttgaatatca ggatataagc aacatacttc ctgcaatttt tgagaaagga
     8881 gaggatcctc ttattctatg tctcgatgga ataaccgatg tcaggaactt cggagcgatt
     8941 gtacggtctg ccgaatgttt cggtgctcat gctgtgctca tacctgaaag aggctctgca
     9001 cgtattactg ccgatgccgt aaagacatca gccggtgccc tgaattcttt tcctgtatgc
     9061 agaaccagaa gtttaacaaa gacaatccaa tttcttaagg agtcgggttt gaaagtgtta
     9121 tgtgcagatg aaaaaagcaa taagagagtt tttaatgcca cactaaccgg gccacttgta
     9181 cttgtgatgg gatcggaaga caggggtata agcagagaat tgtctgccct tgccgatgaa
     9241 actgtcagaa tacctttgaa aggaaagact ggctacctta atgtgtcggt tgcagctgga
     9301 gtaatcctgt atgaaattac aagacagaga gcctcatctg acgggaatct taagaacttt
     9361 tgatttaagt ccaagcttgt taagcttttc tgacattgcc ataatcatct caggagaacc
     9421 ggattttact tcaagttttc ccttaaaatg aactagtacc gcgcactgct ctgcctgtat
     9481 ttcatcatgt ccgcatacct caacaagcga ttttataacg tgcgaaaaag tgttcacgtc
     9541 atcattataa agtatcagag tgcatttttc tgatgcgtca cccttgaatt tttttcttaa
     9601 cggttcacct tttgccatat cagtactcct caaaaagttt tcgtgcaata tcaaccggaa
     9661 tctccttcag ccccagatat gctaccactc tagcgtcgcg gtatccgttc ctcttgagta
     9721 aatcagcata ttcgacagct gtagtgtagt ttataaaaat cccgataaga taaatattat
     9781 tttttttatc atcaacaaga acgtcaaaac cctttccggt tgccagtttc ttaagttctt
     9841 caagctgttg tccggtgagc ggttttggag tcttcattac ctcaaccctg aaaacaagtg
     9901 tcggaggaac ggtatctttc tccgctacaa taggagttat ctcgaacagc ggttttgaac
     9961 cccactcttt ttccagcacc tttgcccgtt ccatagatat ctgctgctta tccatcagag
    10021 ccacaacaaa agcatctttg aatcccaatc ccctcacttt ttgcagtgca acataagcat
    10081 cctccgcttt cctgaacata cctgcataat aaacagtgag gttggattct tctctcctga
    10141 agccatgtac cggagtaaga cctttaaaat atgaaggagc gactggattc cggaaaatcg
    10201 ctatttgaat acggtaaatc aaacctggcg gcacatcggg attaatgggt actttatcat
    10261 ctggagcgta aaccggcttc ttgagtattt caaactgtga atataccggc tccctgctct
    10321 cagcaggcaa attttttata acaggttcga cctcaacctt tttatctgga gccttcacct
    10381 cttgtgccgg caattttgct gctgcctgaa tcttgttatt ttcgacctct ggccccggtt
    10441 cagtcacagt tttctgctta ataactgtat ccttattgcc agcctctttc ccgattattt
    10501 ccggcatttg ttttttttct tcaagaagcc ttgccagcat tgtatcaact tctacaggag
    10561 catacatggc ttctgcctct gcagcctgcc tttcaacacc accacctgca ggaggcttgc
    10621 cttcaggctt ttcctgtgag attttcacct gttctgtttt cttgacggca gcctgaactt
    10681 cttctgcaat acttcctttc ccttcattgc attgtttaat ataatcaggg acatccattt
    10741 cccttgcaac tttccttccg gcactctctg taaacctgtt atatgaaaag acagcctcac
    10801 tgaacatccc tgcaagctgc tgtgcccggc ccagccagaa ccacacatcg gctggaactg
    10861 gcttaatggt tcccgaacct tctgcagcct ccttcaaaag cttaactgcc ctaacaggtt
    10921 cactctcaag cttaaccaga cacactccgc aataatattt gtacagcgga tcccgcggat
    10981 aaagtgccag aagctcggaa aactgccgta aagcatcttc gtatagacct ttattataag
    11041 cttctgcagc tgtctgacgg gttgccctct gttgaacctc ctgcgccgtt acatcagctg
    11101 cacaataaat tacaaacaaa atagaccagg tgaaaaacca tctcttcaaa actctccaac
    11161 ccactgtatg acaattattt ataatcatct gttattcaat tcattgggaa taatttcaat
    11221 aaattcacct atggttatta gttgttaata acaattagtg ctattttttt attatcctgt
    11281 tcaaacttcg taaatttaca ggtaacaaca aaattaatat gtttatggca gatttaaagg
    11341 aaggaatgaa agcaccggaa tttgagggta aggaccagaa cggtaacatt ataaaacttt
    11401 ctgatttcaa aggtaaaaaa ctggttctgt acttttatcc caaggacaac acaccgggat
    11461 gcactgcaga agcatgcaat ctcagggata actataatgt actgattaat aaaggctatg
    11521 cgattctggg tgtaagtcct gatcctgtaa aatcacataa gaatttctcg gagaaatatt
    11581 cactgccttt cccccttatt gccgacactg aaaaagagat tctgaagctt tacggtgcat
    11641 acggtgaaaa gaagatgtac ggaaaaacgg taactggtgt aatcaggaca acgtttgtca
    11701 ttaatgaaga ggggattatt gaaaaaatta taagaaaggt agatacaaaa aatcatgcag
    11761 agcagattct ggataagaat taattgagga acaaggaaaa ataaattaaa tatgagcagc
    11821 gaaaaaaagg aaataaacaa ggaaaagctt aaggcacttg aggttacaat cgggaagatt
    11881 gaaaaagatt atggtaaggg aacgataatg aagctgggtg atcatcctgt tgatagtgtt
    11941 caggtgatat cgacaggctc cattggtctg gatgcagctt tgggcgtatg gggcttgccg
    12001 cgcggcaggg tgattgagat ttttggcccg gaagcatcag gaaagacaac ccttgcaata
    12061 catgctattg ccgaagccca gaaaaacgga ggcatagctg caataataga tgccgaacat
    12121 gcgttcgacc gaaattatgc ggagaagctt ggtgttgatg tggaaaacct tctaatctcc
    12181 cagcccgaca acggtgagca ggcgctcgaa atagccgatc acctgatccg ttcgggtgcg
    12241 ctcgatatca tagttattga ctccgtggca gctctcactc ctaaagccga aattgaaggt
    12301 gaaatgggcg acagcaaaat gggtctgcag gcacggctga tgtcacaggc gctcagaaaa
    12361 ctaactgcca acataagcaa gaccaacact acctgcatct ttataaacca gctcagggaa
    12421 aaaatcggtg tgatgttcgg caaccccgaa acaacaacag gtggtaacgc tctaaaattc
    12481 tatgcttcag tgcgtctcga catcagaaag ataaaccaga taaaagaagg tgaagaaata
    12541 attggcagcc gcaccagagt aaaagtggtt aaaaacaagc ttgctcctcc attcaagaaa
    12601 gcagatttcg acatcctcta cggcgagggc atctcccagg ccggcgaaat tgttgacctt
    12661 ggcgtcgact tcgacataat aaaaaagagc ggctcatggt tcagctacgg cgatacaaaa
    12721 cttggccagg gacgtgatgc tgttattaaa ctcataaaag acaaccccga actgtacgaa
    12781 gaactgaaag caaaagtaat cgaagcgctt aaaaaacaat aaataactat ttattgcacc
    12841 agccatgaaa aaactctttg tcctgatgct tgcagctggg atggtgtttg tattttcatg
    12901 caaacagaaa cccgcaaaag aagctgagct taaacctcct ttcagcgttg aactggcaga
    12961 ctcagagaaa gccagtgggt taatgactcc cgagataatg tggaaattca gccgtctcgg
    13021 ttcttttgca ctctcacccg acggctctga agtggtctat tccattacca gaattgacct
    13081 cgccaccgag gctagaatca caaacctgta taaaataccc accaccggcg gtgaacctgt
    13141 gcagcttact tacgaaggcg gctcaagccc ccagtggttc aataatggga agaaaattgc
    13201 atgggtaagc gaaggtaacc tgatgaccat gaaccccgac ggctcaggta aacagaaggt
    13261 tgaaggactg agtgatttcg aaatatacag aatctcacct gccggaaaca aaatattctt
    13321 tacacggcga gtaaagctcg accagacagc taatgaaaaa cataacctcc cgaaggcaaa
    13381 ggtgagaatc attgatgacc tgatgtaccg gcactggaac tactggcacg actactctta
    13441 cagccatctt ttcattgctt cattcgacgg ggtaaaagta tcggatgaaa aagacatcat
    13501 ggaaggacag aaatttgaat cccctctttc accaaatttc gatgaaaacg aaattacatg
    13561 gagccccgat ggcaggtaca ttgcctatac taccaaaaga ctcaggggca aagaggatgc
    13621 ggtcagcacc aattccgaca tctaccttta cgatgttaca acaggcaaag aagtaaacat
    13681 cagcaagggt aacatgggat acgacaggta tcctgtcttt tcgcccgatg gctcaatgat
    13741 tgcatggcag agcatggaac gcgacggata tgaagctgac cttgacaggc tatttgtcta
    13801 caatattaag gacagcacaa gaacgtgggt aacaaaaggc tgggactttg atgttgaaga
    13861 tattacatgg gctgacaata acactatata cttcacctgt tcatacctgg gcacaaaaca
    13921 ggtcttcaga acagacctca tgagccgtaa ggtaattaaa gtaacggaag gaatgcatga
    13981 tctcggtccg ttaagattgc aatcaggcac catgatagcc cagataatgt cgatgtcgat
    14041 ggcacctgaa attgcctcga tagacctgaa gacaggtgct gttaataaaa taacaaaaat
    14101 aaacgactat atatacgatc acatcaggat gggcaaagtt gaggagaagt acacaaagac
    14161 taaggacaac aaagatcttc aaatgtgggt tatctatcct cccgattttg atccatcaaa
    14221 aaaatatcct ttgcttcttt tctgcaaggg agggcctcag gggcctatga gtcagtggtg
    14281 gtcgtaccgc tggaattttc agatgatggc ggcaaaggga tacatcattg tagctcctaa
    14341 ccggcgtggc agctcagggt tcgggcaggc atggaaagag cagatatcag gtgattacgg
    14401 tggcaaaaac atgcaggatt acctggatgc cacggatgca atggcaaagg aaccttatgt
    14461 tgatgctaac cgcatgggcg ctgtgggtgc cagctacgga ggttattcgg tattttacct
    14521 tgccggcata caccagaaac ggttcaaagc atttgtagct cactgcggta tgttcaacat
    14581 cgaaagctgg tatggttcta ccgaagagct ctggtttcca aacatggaca tcgggggacc
    14641 ttactgggaa aaccatcccg gatacaaatt ctccccacat ctgatggtca aaaaatggga
    14701 taccccaata cttattataa cgggtcagaa cgacttcaga ataccctata ctcagagcct
    14761 tgaagctttc acagcagcac agctgctcgg agtgccaagc cgcttgcttt tcttcgagaa
    14821 cgaagaccat ttcgtatcga aaccccagaa tgcagtcatc tggcaaaggg aattctttgc
    14881 ctggcttgat gaatggctga aataaagcac actgctaagt tcacacgaag cgaagccccg
    14941 ggaatgggca gggcaaacac agtgtagaaa ggtcctgtaa agcggaaggg ttcagggttt
    15001 atgtggtaag taattccata attttttcaa ttttggcttc ctgcggcagg ttgccgtcta
    15061 tccagtggat ttttactcct ttgcgctcca tgcccctgaa ccatgtcatc tgccgctttg
    15121 caaactggtg tattgctatt tcaagctgac ggtacatctc atcgtacgag agctgtccgt
    15181 tcagataaag agtaatatat ttatattcaa gtccgtaata gataagcgtg tcactgtcaa
    15241 tgcctgattc aagcagttcc tgaacttcct ttaccattcc ggcatccatt ctctgtttca
    15301 gcctttctga aatccttttc cttcttgttt ccctgtcgaa aaagaccccg gtgacaagag
    15361 acttaagctt cgggaaatcc ctgataatat cccggtgggc ttcataatac ttttctatct
    15421 caatagctct gacagcccgt ttaacagtgt caatatcagt tttattatgc agtcttttat
    15481 actgtttcag gatctcagtc agttcatcaa gggttttgtt ttcaagttcc ttcctcagct
    15541 cagggttggg cggtacttca gctagtttgt agcccgacac tatactatcg atatacattc
    15601 ccgagcctcc gcatacaacc gggaacttcc cacgtgactt tatatcttca taaactctca
    15661 gaaaatctct ttgaaattca aatacattat atttataacc aggttcaaca atatcaataa
    15721 gatggacagg aatctgccgc ccgtcgacaa catagtccga ataatccttt cctgttccaa
    15781 gattcattct ccggtacacc tggcgggaat cggcgcttat cacttctcct tctatccttg
    15841 cagccagtgc ggcagcaaaa gcagttttgc ctgaagctgt cggacctgta ataattacaa
    15901 ggtcatactc tgttatcatt ccctctggca tgtgtcagct tacttttccg gcaatttacc
    15961 aataaaattg cttatttttg ccgcattctt ttgtgataaa atgaaagcag gccgggaaaa
    16021 tattgtaata cgaaacaaga aggcaacctt tgactatgag cttgaggaaa agctgattgc
    16081 tggaattgtt ctgaccggca ctgaaattaa atcaataagg gcaggaaagg ctgctctgac
    16141 tgattcatac tgtcatttcg ttgacggtga actttatata aaaggaatgc atattgcaga
    16201 atatgactac ggtaatatcc gcaatcatga tcctcttcgt gaaaggaagc ttttactcac
    16261 gaagcgtgag cttcgccggc tcgaaaagaa agtccgggaa gctggttata ctcttattgt
    16321 tacaaaaatc ttcataaacg agcgtggact tgcaaaggcg gagcttgcgc tggcaaaagg
    16381 caagaaaatg tatgataagc gtgaatcgat aaagagaaaa gatgccgcac gcgaaatgga
    16441 acgtgccctt aaagggagag gcggcaagtc ttactaacgt gatttgatta tccttattgc
    16501 ttcacttacc ggaatctgcg tttcaccact atatgctaca acaaatgcat ctttaagtcc
    16561 tgacgcagcc agtcgtttct gaagtgcaac tgcctcttca tatacagaga atgaacccac
    16621 ctgatacttg tagacatttc cgaccttaac cataacaagt ttttctccgg gccaaagccc
    16681 cagctttttt gcaacctgtt cagctggcag tggagtggaa tatgatgcaa cctgtactct
    16741 gaactctata ttcctatcag aaggtgctgc ttgtctgcca cgcggttctg atacaacatc
    16801 tcctctgcca tcctgcatca catttctttt tcctgcctct gtgtttactg cttcagatgt
    16861 ctgagcacct tttattgaca cctttatatt attcatactg aaggttcgtt ttgtttttcc
    16921 gccggtaatc ataacaagcc tgccttccag atcaaagctt ccgctcatcg atttccccgg
    16981 cataacaaga tacgagaagc ttattttctt gctggcaggt atggtaagcc agacaatatt
    17041 aagttgatta ttgtaccagg aaaaatcacc tgcaggaata ttttcaggtg caacttcaac
    17101 accttcagga aaatcctggg taaagcgtac cagaccatca gaggcatcga atgttacatc
    17161 aaccgaaaca tggttatact gtccggcaat ccatacagtg tcggttttgc agacaatttt
    17221 cacctgcccg gcaaggggtg tcaaaacaaa tatgaaaaaa cagaaaatga agcttaagta
    17281 cttcatcaca aaatcagaag ttcgggctca ggaggtatct cgagtagaac ttgtcgatca
    17341 ggtctacagc ctcatcagca gtatccacta cggtgaacag gtctagatcc tgaggtgaga
    17401 tattgtgttc ctgctcaagc attacctctt taatccattt aataaggcct tcccagtatt
    17461 ttttgcccac cagtataatc gggaattttc ctatttttct tgtctggata agggtcattg
    17521 cttcaaacag ctcgtcgaaa gtgccgaacc ctccgggcat tactatgaaa ccctgggcat
    17581 attttacaaa catgactttt ctcacgaaga aatggtcgaa ggttatgagt ttgtcgctgt
    17641 caatgtaagg attcggtttc tgctcgaatg gaaggtcgat attgatacct accgatttgc
    17701 ccttgcccct ccatgcacct ttattggctg cttccattat tccagggcct ccgcctgtaa
    17761 taacaccata acctttctgt gtgagcttaa aggcaatctc ttctgccatc ttgtaatatt
    17821 tgttgtcggg ctttgtgcgc gcagaaccga atattgaaac gcacggccct attctggcaa
    17881 gtttttcgaa tccttcaaca aactctgaaa gtattttaaa aactctccac gaatcggtaa
    17941 tatgaatctc ctgccatgtc ttttgttcga aagcatcttt gataagatca gcgggtttct
    18001 ccatagttcc ttatttaaaa gttttcaaat ataataattt atcaataata gccttaaaaa
    18061 cagcttttga tagcctttgt ttttaagctt ttgattattt atgcactgtc ttctcaagtt
    18121 cccttttcag aaacgctcct gtataactct tctcacattt aattatttct tctggtggtc
    18181 ctgaaaaaac aacatatcct ccctcctttc cgccttccgg tccgagatct attatataat
    18241 ctgccatttt aataacctcc atgttatgct caatcacaat aactgtattg cctctgtcaa
    18301 ccagtctgtt cagaacgttc agaagcactc tgacatcttc aaaatggagg cctgtggtag
    18361 gctcatcaag aatatacaaa gtcttaccag tatcgcgccg ggcaagttca gtagccagtt
    18421 ttactctctg cgattctcct ccggaaaggg ttgttgactg ctgtccgatt ttaatatatc
    18481 ccaaccccac atcctgcaat gtttttattt tatgatatat ggcaggaaca tttgaaaaga
    18541 actcaactgc ctcatcaaca gtcatttcta gaacatcact gattgacttc cctttgtatt
    18601 ttacttcgag tgtctccctg ttgtaacgtt ttccgttgca ttcggtacag agcacataca
    18661 catcgggaag aaaattcatt tcaattgttt tcactcctgc gccttcacag ccctcgcatc
    18721 ttcccccttt tacgttgaac gagaatcttc ccggaccgaa tcctcttatt ttggcttcgg
    18781 cagtctgagc aaagaggctg cgtatatcgg taaaaacacc cgtatatgtt gcagggtttg
    18841 agcgtggtgt tttcccaata ggtgcctgat caacttcaat aaccttatct atatgctcag
    18901 tacctataat atcatggtat ggtagtggtg ttttacccga gttgtgatat ttccttgcga
    18961 gcgcaggatg gagagtatgg tttataaggc tcgatttccc gctgcctgaa acacctgtaa
    19021 cgcatataaa catgcccagt ggaaatgcta catcaatgtt tttcaggtta ttacctgagg
    19081 cgcctctgat tataagatat ttgccattac ctctccgccg ctcatccggt acttcgatac
    19141 gtttgcagtt gctcaggtaa gcggcagtaa gtgtttcaca gtgcataaat tcctgtgggg
    19201 ggccctgggc aacaatcctt ccgccgtgta caccggcacc cggacccaca tcgatgatat
    19261 ggtcggccga cattatcata tccctgtcgt gctcaacaac aatcactgag ttaccctcat
    19321 cgcgaagttt cctcagtgat gagatgagtc tgcggttgtc gcgctggtgc aatccaatgc
    19381 tgggctcatc aagtatgtac agtacattta caagtttcga tcctatctgt gttgccagcc
    19441 tgattctctg gctctcacca cctgaaagag tgcgtgcact cctgttaagc gataaataat
    19501 caagacctac ctcgagcagg aaactgagcc ttgcccttat ctctttaaga atctctgatg
    19561 ctatcttctt ttgcttttcc gacatctttt cttccacaga agccaggaaa gagtacagct
    19621 cacgtatatc catcgccgca agctcaccga tgtttttatc tgcaatcctg aaccagagtg
    19681 cttctttttt aagtctgtta ccattacatt ccggacatgt ccggtattct acatattgat
    19741 ctttcaacct cgagccgttt ctctgtccgt taataacctc aagattgccc agaaagctga
    19801 taactccttc gaaagtcaga aaatagtttg aagttagtcc cagtggcgta ttagaaagaa
    19861 tgagaacttc gtcggagccg aaaagtattt tgttcacaac gtcttctggc aattcgctta
    19921 taggagtatc gatggtatat ccgctttttt caaggatagc ttctatctgc cagaatatcc
    19981 atgtattacg gtacttgccc agtggttcta ttccaccttt tcttatactg agcgacctgt
    20041 cgggtatgat tttgttaatg tcaatgtcgg atatttctcc aagtccgttg caatgggggc
    20101 aggcaccctg aggtgaatta aatgaaaatg tatgtggggc aggctcgttg taagagatgc
    20161 ccgttacagg gcacatcaga tgacggctga aatatcttat tttatctgat tccctgtcga
    20221 gaatcatcat cacaccatca ccctgatcta gagcaagaag aacggattcc ctgaggcgtt
    20281 tatgagagac ttcccccact ctgaacttgt caataacaat ttctatgaaa tgagttttgt
    20341 aacgatcgag tttcatcccg ggagtaatct ctcttatctc accgttaatc cgtgcataga
    20401 gatatccttt caacctcagc ctttcaaaca gctccctgta atggcccttt ctgcccctta
    20461 ccaccggggc aagaatgttg atttttttac cgtgaaaacc cttttcaata agttcaatta
    20521 tctgctcgtc gttataacgt accatttttt cacccgatgc atatgaatag gcatcagcag
    20581 cccgtgcata gagcagtcgg aggtagtcat aaatctctgt tattgtaccg acggtagacc
    20641 ttgggttttt gcttgtagat ttctgttcaa tggatatgac cggactgagt ccggttatct
    20701 tgtccacatc gggccgttcc aagcctccca gaaactgccg cgcatatgcc gagagagtct
    20761 ccatatagcg tctctgaccc tcggcataga ttgtatcgaa tgcaagcgac gacttcccac
    20821 tcccgctcag acctgttata acaacaagct tgttgcgggg aatcgtaaca tctatatttt
    20881 tcagattatg aacccttgct cccagaaccg taagcctgtc gctcattaca gttgcaattt
    20941 cctttcagtg attaaatttc attttcgtaa agagtggttc ttgaaccctc ttcgggtatc
    21001 ttgatatagt aactctttcc tgatttcttc gatagatagg gctttcttag ccacggattg
    21061 tagaatttca gaattttgta attcgttccg aaatgtgctg caaatcttgc aaaatctgca
    21121 acagctgtat ccaccttcac ctcagtgtag cgcaatggtg ggtatagttc ttctttatcg
    21181 atctcgaaac catatgctgc cggattgctt ataataattt tatatgacag gattctgaag
    21241 atataccttg cggtttcctc agcaagcagg agatcataat agttagtctg tttctgaatg
    21301 gaaatctgtt cgtctatggc atttcttcca ccattgtacg atgcggcagc aagtgtccag
    21361 ttgccatact tttcataaga ttttttaaag taatcgcatg caactacggt ggatttttca
    21421 agatgatacc tctcatcaat ttcgttgttt atttcaagtc cgtgctccct gcctgtcccg
    21481 gcaagtatct gccagaagcc taccgctcct gctggtgata ccatgttact gagctcgctc
    21541 tctgcaacag ctacatactt aaaatcatca ggtattccat tctcactcag tattttctca
    21601 atcaccggaa aatatcttgg agctctttta attatcataa ttgtagagcc atggcgatag
    21661 gccgtcataa taatttctct ttcaagactt tcgcgtgtat caaaattttc taacggcact
    21721 ctttcaccgg caaaataaat tgtatcggga agttttatat ccagcataaa tggacttttc
    21781 ccatttaccg ggagtttttc aggtctggag tcattaaaaa agcctgataa aagaaagaca
    21841 gcaccaataa acagagccgg cagcagcaaa taataaatgt ttctgggtgg tttgtatctc
    21901 attctatttc attttaaaat gaaatg
//
LOCUS       DAOSNB010000010        20210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2660_length_20210_cov_4.638402, whole genome shotgun sequence.
ACCESSION   DAOSNB010000010 DAOSNB010000000
VERSION     DAOSNB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2660_length_20210_cov_4.638402"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1627
                     /gene="hcp"
                     /locus_tag="PLG42_01080"
                     /gene_synonym="priS"
     CDS             2..1627
                     /gene="hcp"
                     /locus_tag="PLG42_01080"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOU29832.1"
                     /translation="MFCNQCQETAKNTGCTINGVCGKKEKTSDLQDLLVYACQGLAFA
                     TTEARKRGIKTDTESRHIVNSLFMTITNANFDDRSIIKAIQDCLVYRDSLKERVNIPF
                     LHESVRWSASSETEFYEKAKQVGTLTYDTNEDVRSLKQYVLFGIKGLAAYAEHASNLG
                     FEDPEIFDFIEQSLIMITKSENVESMMQWLVSTGEHGVKVMALLDKANTSTFGDPEIT
                     RVNIGTGKNPGILVSGHDLKDLEELLIQTEGQGIDIYTHSEMLPAHAYPGLKKYKHLR
                     GNYGNAWHRQQDEFESFNGPILFTTNCLVPPRKTATYNNRVFTTGPTGMPEWKYIDKR
                     LPNGQKDFSEIIELAKKCPPPSEIENGELVIGFAHNQVLALADKILEAVNSGAIRKMI
                     VMSGCDGRMKSREYYTEFASKLPKDTVILTSGCAKYRYNKLNLGNINGIPRVLDAGQC
                     NDSYSWAFVALKLKEVLGLDDINKLPLAFNIAWYEQKAVIVHLALLYLGFKNTRIGPT
                     LPGFLTPNVLKIIQEKFGVQTITSVEEDLAKIL"
     gene            1726..1905
                     /locus_tag="PLG42_01085"
     CDS             1726..1905
                     /locus_tag="PLG42_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOU29833.1"
                     /translation="MEEDVLICNCNEVYRSEIVKAIKEKGLKTVEEVGEVTLAGTGCG
                     GCQEDIQAILDEING"
     gene            1995..2720
                     /locus_tag="PLG42_01090"
     CDS             1995..2720
                     /locus_tag="PLG42_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOU29834.1"
                     /translation="MKEPISYFFEKDGFNPSIVRRYALGERFAGIMLNDGRIGICAVL
                     DAQLDDSILSGRKQPDISNHGHRVVLNAYYNALYNYSTELPCNSDILNRVNLRNFRNI
                     VIVGYFESMVRKLRDAGISFRVFDKDENIEGEGISPINELPDALGKADAVIITGSAIA
                     NNTFCDLTGKTGSGCSVFLLGPSNILHPDMFNYKNVKVIFGSVFERYEERILDMIDAG
                     YGAKDFLTDKNKVCMNHHSFKLL"
     gene            2717..3307
                     /gene="thiF"
                     /locus_tag="PLG42_01095"
     CDS             2717..3307
                     /gene="thiF"
                     /locus_tag="PLG42_01095"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOU29835.1"
                     /translation="MKADEVREHLKKFRVGIAGAGGLGSNCAMLLARAGVGTLVICDF
                     DTVERSNLNRQFYFLHQCGMKKTEALKENLKMIDEDINVIARNIKLERENIPEVFAGC
                     DVIVEAFDRAEMKEMIIEAVQTDMPGVPLIVASGLAGWGRNDSIRSRKIDDNLYVCGD
                     ESQEVSEDNPPLAPRVVIVAAMQANFVVEILMNRKK"
     gene            3314..3517
                     /gene="thiS"
                     /locus_tag="PLG42_01100"
     CDS             3314..3517
                     /gene="thiS"
                     /locus_tag="PLG42_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOU29836.1"
                     /translation="MKILLNNRVEEFDAGSLTVQQLLEIKKYSFRMRTVKVNGRYVPP
                     AEYDKTIINDGDNVQVIYLMSGG"
     gene            3553..3885
                     /locus_tag="PLG42_01105"
     CDS             3553..3885
                     /locus_tag="PLG42_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOU29837.1"
                     /translation="MRIRFKYADKIPPFLRNKYILAIIIFLLWIILLDANNLIDRIKQ
                     MNQLRKLEKEKEYYTKKIEEEREKLNRLRTDDGYLEKFAREQYLMKKKDEDLFIVLTP
                     REERKRRR"
     gene            complement(3889..5139)
                     /locus_tag="PLG42_01110"
     CDS             complement(3889..5139)
                     /locus_tag="PLG42_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29838.1"
                     /translation="MWSADKKLFPALATEPPHPETGIIVVVPACGEPDIIKLLDSLAS
                     CFQPQCHTEVFIIINAPPGASAEVIENNLLAISNIEAWRKKAGALFFRLYVVDIGQPS
                     YKKWGVGMARKTGMDEAFSRFQSIGKMNGVIVSLDADCTVQQNYFQAIEKELFNHSGR
                     RACSIYFEHPLSGKEYPQELYNAVVRYELHLRYYYQGLLHTGYPWVYHTVGSCMAVKA
                     HAYAQAGGMNRRQAGEDFYFIQKLLPAGGYFSLNSTTVYPSPRISDRVPFGTGAAVKN
                     MVEKGMENFLTYNPHAFKDLRIFFGKVNKSSGSDLNELLKLYNDLPDAVCQFVDPYEW
                     EKKIKEIKQNTSSPASFIKRFFGWFNMFRIIKFLNFAHNKGIYRKLSPGEATKKLFEL
                     KGKWDVPQDERELVIYLRNMERNE"
     gene            complement(5158..5757)
                     /locus_tag="PLG42_01115"
     CDS             complement(5158..5757)
                     /locus_tag="PLG42_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU29839.1"
                     /translation="MKNLSTILLIVLFLAVAGIYVLHFTGTGNGKRVATAEGAGESKS
                     HGIVYVNIDTIIFNFEMFADRRNDLLNKQKNAEAELNSKTFQYEKGVRDYQEKVNKGL
                     VTRATAAQMEQALLQQQQDLVALRDKLQSELIEEEQVMNRQILDYITKFLEENKSEYN
                     YQFILGKSFGGPVLYSDSTLDITYKVLNALNEKYRSEKK"
     gene            complement(5839..6825)
                     /locus_tag="PLG42_01120"
     CDS             complement(5839..6825)
                     /locus_tag="PLG42_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU29840.1"
                     /translation="MLKRFIYLIVFIFLAINTFGQDPTFSQFYANPLYLSPSFAGATE
                     EYRLGINYRNQWPAIPGVFETYSISFDKAMPNFSSGIGVLATYDVAGSGDLSTTNIGL
                     LYSYDFNITRDWHVRPGVHFKFYYLGLNIYKLIFNSQITGTGTTPPVTPPPFDKVADV
                     DFATSALVYNDAIWAGFTLDHLFTPKQSFYGDEAQLPVKFNLYGGVRIVGTTRLRTKM
                     IEALSVAMNFQTQSRYYQTDIGLYYYKSPLIFGLWYRGIPFMTSQAGDAIIGLIGIKT
                     DKLHIGYSYDFTISNLISSTGGAHEISIVYEFTSFEITGGRKRIRAIPCPEF"
     gene            complement(7014..8315)
                     /locus_tag="PLG42_01125"
     CDS             complement(7014..8315)
                     /locus_tag="PLG42_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU29841.1"
                     /translation="MGDRRHMELDELFRSKLDNIEAVPDPAVRHSLMRKLAVKEFLRF
                     NPVRFNIYYAAGIVAAGIAAAVLLFSHDDRAEKSEQGTINIKSEIRSDTVSMFYSPAV
                     IRKESQEKTEKQAISQSVVKPDERKRGVQPPQENKSVGVMAADSLKKMEFSPVVSDKT
                     TNANIRKTPVASFKASFLQGCMPLKVSFYNTSVAYDSCKWIFGDGGSSVSVNPEWIYD
                     RPGAYRVVLNVYGGGTVSYATEVITVHPKPSARFEVEPLNPTGGNDAVKFINYSNDAV
                     RYRWDLGDGTVSFAFEPIHRYDRTGKYNIKLTVWSEFGCADSITVINALGGSDYFIEF
                     PNAFIPNRDGPTGGYYSATSDASAHIFHPIASGVVEYQLRIFTRTGILIFESNDINIG
                     WDGYNKGNLCEPGVYVWKVRVTFKNGETYVKMGDVTLLRGN"
     gene            complement(8315..8899)
                     /locus_tag="PLG42_01130"
     CDS             complement(8315..8899)
                     /locus_tag="PLG42_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU29842.1"
                     /translation="MLSENEIIEGCARHDRRAQQMLYDRYSRLLFGVCMRYASDRAEA
                     EDILQESFLKIYFSIRDYSGTGSFTGWMRKIVVNTAITHYHRNLKHKHYVEIEDYVSV
                     ETGVSSFEEDFFTSDELFKVLNELPPGYRMVFNLYAIEGYKHKEIAEMLGIDINTSKS
                     QYSRAKAALREKLEVLRKLKEKYNAEDNIADLQK"
     gene            9117..10028
                     /locus_tag="PLG42_01135"
     CDS             9117..10028
                     /locus_tag="PLG42_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOU29843.1"
                     /translation="MINKLIASTLPYFPKKFIWLFSRTYISGETIDDAMRVSAELNRQ
                     KIKVTIDVLGEFIKNLDEAQKNKEEYLNLIDVTTRHGIDGNFSLKPTSFGLLIDKEIC
                     YKHIHEIVAKAASVNNFIRIDMEDSSCTDLEIELYRRLKAEFPKSVGLVLQAYLHRTR
                     SDIEKLLEIHTPDSPLNFRLCKGIYIEPETIAYKKYEEINMHYLEDLELMLKNKIYVG
                     IATHDKPLIDGAYDLIKKYDVPRNMYEFQMLYGVTPKLRENIVNEGHTMRVYVPFGKE
                     WFGYSTRRLKENPKIASHIIKALFWKG"
     gene            complement(10054..10773)
                     /locus_tag="PLG42_01140"
     CDS             complement(10054..10773)
                     /locus_tag="PLG42_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29844.1"
                     /translation="MRPGWVVAIILINLTFVASAQQSELSGRSILFRGVVIDAETQAR
                     IADSKIYLNGAVKTVTGTDGTFSFLARPNDTVVFRAMGYQLARIIVSDTLRATEFLAG
                     IYLKPDTISIGEVIILPRNRSLKTELMASPVPSDVKTEYARSNVNIAAFQGRTGQYKL
                     GDPEMNYELLRQVQKKEAYEKGGIPSDRIVGISPLLLLPAAYLILHGLPEPPAPPEPQ
                     ISQKELEELNKIFLEKIRNKK"
     gene            complement(10782..11354)
                     /locus_tag="PLG42_01145"
     CDS             complement(10782..11354)
                     /locus_tag="PLG42_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU29845.1"
                     /translation="MDFSELVSKCRSYRRFDESFRIDYELLKKLIGYARLSASAANRQ
                     PLKYLIYNKPEDCEKVFPCTAWAGCLPDWPGPEPGERPAAYIIILGDTTITESFGIDP
                     GIAAQTIMLGAVNEGLGGCMIASIKKDKLREELQIPLQYEILLVLALGKPVEKVVIED
                     IRGDNVKYWRDENNVHHVPKRTLDELILKL"
     gene            complement(11410..12264)
                     /locus_tag="PLG42_01150"
     CDS             complement(11410..12264)
                     /locus_tag="PLG42_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOU29846.1"
                     /translation="MKLTLGFTPCPNDTFVFHAMVNRKIDTEGLEFDYFLADVEELNR
                     RAFEGTTDILKISYHAYAYAADKYLILDSGSAIGRGNGPLLISKREIHEDEVKNLLIA
                     IPGKYTTANLLFSIKWPEALNKKEYVFSEIENALLNGEVDAGIIIHETRFTYFRKGLR
                     KIVDLGRYWEEVTGYPVPLGAIVINRAVPEDIALKVNRILKKSIEYAYTDLYSTFDFV
                     RQHAREMESDVLHSHIEMYVNEFTAELGLEGRRAVEKLFSMASEAGVIPEPPARIFLT
                     REQAELDN"
     gene            complement(12268..12954)
                     /gene="mqnB"
                     /locus_tag="PLG42_01155"
     CDS             complement(12268..12954)
                     /gene="mqnB"
                     /locus_tag="PLG42_01155"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOU29847.1"
                     /translation="MNCKDNIIVKILLIFATDHEAECIKEGKGEEPFRAGPHEVSVLV
                     TGVGSVATAWALKKWLQNYGNPDLAINAGIAGTFNENISIGEVVMPVSDCFADLGIET
                     DTGFVPLKDTYLMESDKFPFSGGNIICDNEFTGRIKNFFRPVRAVTVNMSSGSEKTIE
                     RLKAHFNPDIETMEGAAFFYVCSMEKIPFIALRAISNRVGAAERKNWNIPLAINNLSE
                     KLKELLTLLQ"
     gene            13021..13452
                     /locus_tag="PLG42_01160"
     CDS             13021..13452
                     /locus_tag="PLG42_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOU29848.1"
                     /translation="MGQKLYLTRREKFSAAHRMYVPEWTDEQNLDVFGKCSNPNWHGH
                     DYVLYVTVRGDISGETGLVINMNVLKEIIREKIIDKVDHRNLNLEVDFMKGMNATTEN
                     LAVAIWNELEEALKSAGCELYCVKIEETENNTVEYYGKKDH"
     gene            13433..14083
                     /gene="folE"
                     /locus_tag="PLG42_01165"
     CDS             13433..14083
                     /gene="folE"
                     /locus_tag="PLG42_01165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOU29849.1"
                     /translation="MAKKIIKVLENGVEETIKGYIKIEQWNKENVDELASHYYKVLGL
                     LGEDPDREGLKKTPERVAKALYFMTSGYHQNPCKILNSAKFREDYKQMVLVKDIDIFS
                     LCEHHMLPFFGKAHVAYIPNGYITGLSKIARVVEAFSRRLQVQERLTTQIRDCIQGCL
                     EPLGVAVVIEAQHLCMMIRGVQKQNSVTTTSAFTGCFLDNFRTREEFMHLIGTKLH"
     gene            14145..15026
                     /gene="fabD"
                     /locus_tag="PLG42_01170"
     CDS             14145..15026
                     /gene="fabD"
                     /locus_tag="PLG42_01170"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOU29850.1"
                     /translation="MKAYVFPGQGSQYPGMGKDLYEKYPLAKELFDKANEILGFRITD
                     LMFNGTEEDLKQTKVTQPAIFLHSTIIIKVMGDAFSPDMVAGHSLGEFSALVANRTIS
                     FEEGLVLVSKRAAAMQKACEMNPSTMAAILGLDDSVVEEVCAGFNGLVVPANYNSPGQ
                     IVISGTFEAIDQAIEILKAKGAKRALKLQVGGAFHSPLMEPARKELEEAINSTPFKKP
                     VCPVYQNVNAKPSTDPGEIKANLVMQLTSPVRWTQTIINMINDGATSFTEAGPGNVLQ
                     GLIKKINRDIEVAGAVV"
     gene            complement(15052..18030)
                     /locus_tag="PLG42_01175"
     CDS             complement(15052..18030)
                     /locus_tag="PLG42_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOU29851.1"
                     /translation="MAVIDGILEMEKYDFTDTSFDLLMQKRIHRVLIICSNYDNYMLE
                     EDGRIEEQIFNEYVSLNLRYPPTFIQTDNVEDAFKILAQGNIDLVIAMLSVKGTDVFA
                     LAKQIKSIYPKIPIVVLTYFSREVSVRLEKEDLSAIDYVFCWLGDASLILAIIKLLED
                     RMNADHDIEQVGVQAIILVENSRRYISSYLPSLYKIILAQSLDFQREALNEHQRMLKM
                     RGRPKILLATNYEEAVELYQKYKYNVLGIISDISYKRNGEIDENAGIELCKMVKADDD
                     QVPFLLQSSSLEHKKVAEELGAGFIYKYSKSLSLELRNYLIQNLAFGPFVFRNPDTME
                     PIAIATDLQSLQQKILTVPDKSLEYHASRNHFSKWLNARALFPVAQLFKYLRKEDFET
                     LDEMRRFLYVAISSFRLGKGRGVIAKFDRSNFDEYQIFSRIGEGSIGGKARGLAFINS
                     IIKKNKLYNKFPDVIITIPRTIVLSTDVFDEFMETNNLYSIAMSDLPDEDILARFISA
                     ELPGWLYQDLYAFLTVSRNYPLAVRSSSKLEDSYYQSFAGIYSTYMIPRVPDNRYMVQ
                     LLADAIKEVYASVYYRMSKAYMAATSNVIDEEKMGIIIQEVCGTRHETMFYPTFSGVA
                     RSLNYYPIGSEKAEDGIANVALGLGRLVVEGGQTLRFSPKHPKKILQLSSPEMALRDT
                     QKFFYALDLNVNRFVPSTDDGVNLLKVKISDVNNETAMRYLASTYDRNNNILYDGIGR
                     PGVRIITFASILQYNQFPLAEILNTLLEIGSEEMNHPIEIEFAANLETPPGTPKIFYF
                     LQIRPIVLNEQLYKVDIDKVNPEDTIIYSESALGNGVFKDIRDIVYLKPETFNPAYSE
                     KIAVDIEKINTSFQRNGEGYVLIGPGRWGSNDPWLGIPVKWPQISAARVIVEAGLKDY
                     RIDPSQGTHFFQNLTSFRVGYITVNPYNGDGHFDVDWLNQQQVVYENQFIRHVRFEKP
                     LEILIDGRKHKGVVMKPSSN"
     gene            complement(18178..19200)
                     /locus_tag="PLG42_01180"
     CDS             complement(18178..19200)
                     /locus_tag="PLG42_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOU29852.1"
                     /translation="MEDKSDLKYSPKDFKSDQEVRWCPGCGNHAILASIQKALSELGV
                     PKEKYVFVSGIGCSSRLPYYMNTYGFHGIHGQAAAIATGVKTVNPDLSVWVITGDGDS
                     LAIGGNHFIHAIRRNIDINIILFNNEIYGLTKGQYSPTSNKGLVTKTSPFGTVEEPFS
                     VGELVIGAKGKFFARTVDNNINLTTEILVEAARHKGTSVVEVLQNCVIFNDGIHDVVA
                     GKDVRDDRTIILRHGEPMLFGKDRKSGLILEGLKLKVVRIGEKGIKEKDILVHNAHEP
                     NPGIHYFLANMRYPEYPVALGVIRSVEGETYEAAVAEQIKRVKEKSEIKCVDDLLESG
                     TVFVVE"
     gene            complement(19202..>20210)
                     /locus_tag="PLG42_01185"
     CDS             complement(19202..>20210)
                     /locus_tag="PLG42_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOU29853.1"
                     /translation="KDLGVISFQGEDEIAGICSAIGASFAGCLAVTSTSGPGLALKTE
                     GIGLAVMAELPLVIVDVQRGGPSTGLPTKTEQADLMQALFGRNGECPVVVLAASTPTD
                     CFHYAYQAAKIAIEHMTPVLLLSDSYLANGSEPWHIPKMSELPDIKPPYARKENEPYI
                     PYKRDEKRLNRYWAIPGTPGMEHRIGGLEKTIKGTVSYVPENHEYMVKMRDEKVKRVA
                     EKIPPLNVYGDETGDLLLVGWGSTYGHMLTSVKELREEGYNVSLACFNYIYPLPSNVR
                     DVFSKFKKIAVCEINLGQFANYLRMNFQQFHFYQINKVQGLPFTVKEIKENCIKLLEI
                     K"
BASE COUNT         5957 a         4346 c         4022 g         5885 t
ORIGIN      
        1 catgttttgt aatcaatgcc aggagacggc aaaaaacaca ggctgtacaa ttaacggtgt
       61 atgtggtaaa aaggaaaaaa cctctgatct tcaggacctt cttgtatatg catgtcaggg
      121 acttgcattt gcaactactg aagcccgtaa acgcggaatc aaaacggaca ccgaaagcag
      181 gcatattgtc aactctcttt ttatgacaat caccaatgcc aacttcgatg accgttctat
      241 aatcaaagca atacaggatt gtctggttta cagggatagt ctgaaagaga gggttaatat
      301 tcctttcctg cacgaatcgg ttagatggag tgcctcatcg gagactgagt tttacgaaaa
      361 agccaaacag gttgggacat taacctatga cacaaatgaa gatgtaagat ccctgaagca
      421 atatgtccta ttcgggatta aaggattggc tgcgtatgcc gagcatgcat ccaacctcgg
      481 ttttgaagac cctgagattt ttgattttat agagcaatct ctcataatga ttacaaagtc
      541 tgaaaatgtt gaaagcatga tgcagtggct ggtgagcaca ggtgaacatg gagtaaaggt
      601 gatggctctg ctcgataaag ccaatacttc aacatttggc gatcctgaaa ttaccagagt
      661 aaacataggt accggtaaaa atccaggcat tctggtaagc ggtcatgacc tgaaagacct
      721 cgaggaatta cttatccaga ccgaagggca gggaatagat atttacacac attccgagat
      781 gctcccggca catgcgtatc ccggactaaa gaaatacaaa cacctcagag gcaattacgg
      841 taacgcatgg caccgccagc aggatgagtt tgaatcattc aacggtccca tactatttac
      901 taccaactgt ctggtaccac cacgaaaaac tgccacatat aacaatagag tctttacaac
      961 cggacccaca ggcatgcccg aatggaaata tattgacaaa agattaccga acgggcagaa
     1021 agatttttca gagataatag aacttgcaaa gaaatgcccg cctccatcag aaattgagaa
     1081 cggtgaatta gtaatcggtt ttgcacataa ccaggtactt gcccttgccg ataaaatact
     1141 cgaagccgta aacagcggtg ctatcagaaa gatgatagtg atgtcaggct gcgacggaag
     1201 gatgaaatca agagaatatt acaccgaatt tgcttcaaag cttcctaaag atacagtcat
     1261 actgacttcc ggatgcgcaa aataccgtta caacaaactc aacctgggca atattaacgg
     1321 aattccgaga gtgctcgatg ctggccagtg caacgactcc tattcatggg cttttgtagc
     1381 actgaaacta aaagaagtgc tcggccttga cgacatcaac aaactgccgc tggcattcaa
     1441 tattgcatgg tatgaacaga aagccgtaat tgtgcatctt gcactcctct accttggctt
     1501 taaaaacaca cgcatcggac caacgctacc tggcttcctt acgcctaacg tgctgaaaat
     1561 aattcaggaa aaatttggcg ttcagactat tacttcagtt gaagaggacc ttgcaaagat
     1621 actttgacaa aaattgtaaa tttaaatggc gcaaatcgtc catatgcgtt ttacaaaaca
     1681 aaaacaaaac agtattgtta gtattaataa ccagatcata aaattatgga agaagatgtt
     1741 ttaatttgta actgtaacga agtttacagg agtgaaatag tgaaagctat taaagaaaaa
     1801 ggactcaaaa cagttgagga ggtaggagaa gtaactcttg cggggacggg ttgcggaggt
     1861 tgccaggaag atattcaggc gatactcgat gaaataaacg gatagtggtt atttttccgt
     1921 agccttttta aatcccggca accgatggtg ccgggatttt ttatctttgc aattaataaa
     1981 tctgccgtaa cctgatgaaa gaaccaataa gttacttttt tgaaaaggat ggatttaatc
     2041 cttcaatagt aaggcgctat gcactcgggg aaagattcgc aggcataatg ctcaacgacg
     2101 gcagaattgg catttgtgct gttctcgacg cacaactcga tgattcgatt ctttcaggca
     2161 gaaaacaacc agatatatct aaccacggtc acagggtggt attgaatgca tattacaacg
     2221 ccctttacaa ttattcaact gaattgccct gcaattccga cattcttaac agggtgaatc
     2281 tcaggaactt caggaatata gtaatagtgg gttatttcga atcaatggtc aggaaactga
     2341 gggatgcagg gatttctttc agggtctttg acaaagatga aaacatcgaa ggcgaaggta
     2401 tttcacctat aaatgaactg cctgatgcat tgggcaaagc cgatgcggtc attattacag
     2461 gatctgcaat agccaataac accttttgcg atctgacagg caagacaggt tcagggtgca
     2521 gtgtatttct tcttggtcct tcaaatatcc tccatcccga tatgttcaat tataaaaacg
     2581 taaaagttat tttcggctcg gtttttgaaa gatacgaaga gagaattctt gatatgattg
     2641 acgcaggata tggggcaaaa gattttctga cagataaaaa caaagtatgc atgaatcatc
     2701 attcatttaa actgttatga aggccgatga ggtgagggaa catcttaaga aattcagagt
     2761 aggtattgca ggtgccggcg gactgggttc aaactgtgcc atgctcctgg caagagccgg
     2821 cgtgggcacc ctggttatct gcgattttga cacagttgaa agaagcaacc ttaaccgtca
     2881 gttctatttc cttcaccagt gtggaatgaa aaaaactgaa gcattaaagg aaaacctgaa
     2941 aatgattgat gaagatatta atgttattgc ccgcaatata aaacttgaaa gagaaaatat
     3001 tcctgaggtt tttgccggat gtgatgtaat tgttgaagct ttcgacaggg cagaaatgaa
     3061 agagatgatc atcgaagctg tacaaacaga tatgcccgga gttcctctta tcgttgcttc
     3121 aggccttgcc ggatggggaa gaaatgattc aatcagaagc aggaaaattg atgataacct
     3181 ttatgtgtgc ggtgatgaat cacaggaggt ctcagaagac aatcctccgc tcgcaccacg
     3241 ggtggttatc gttgctgcca tgcaggcaaa ttttgttgtt gaaatcctga tgaacagaaa
     3301 aaaataggct gaaatgaaga tattgctcaa taacagagtt gaagaattcg atgccggaag
     3361 ccttacggtt caacaactgc tcgagataaa gaaatacagc ttcaggatgc gcaccgttaa
     3421 agttaacgga cgttacgtgc ctccggcaga atacgataaa acaatcatta acgatggcga
     3481 taacgttcag gttatttacc tgatgagcgg tggctgaaaa ttatctgaaa taatttattg
     3541 taattttgaa gcgtgagaat ccggttcaaa tatgctgaca aaatccctcc atttctgagg
     3601 aataaatata ttttagctat aattattttc cttctttgga tcattcttct cgatgcaaac
     3661 aatctgatcg acagaataaa gcagatgaac cagctgagga agctggagaa agaaaaggaa
     3721 tattatacta agaagattga agaagagcgt gaaaaactca acaggttaag aacagatgac
     3781 ggttacctcg agaaatttgc cagagagcaa taccttatga agaaaaaaga cgaagacctg
     3841 tttattgtgc tgactcccag agaagaaaga aagagacgca gataaccctc actcatttcg
     3901 ctccatgttt ctgagataaa taaccagttc ccgttcatcc tgaggaacat cccattttcc
     3961 ttttaattca aaaagttttt ttgttgcttc accgggagac aattttctgt aaatcccttt
     4021 attgtgtgcg aaattcagaa acttaataat gcggaacatg ttaaaccaac cgaaaaatct
     4081 ctttatgaaa gatgccggcg atgaagtatt ctgctttatt tccttgattt ttttctccca
     4141 ttcataggga tcaacaaact gacaaaccgc atctggcaaa tcattgtata atttcaaaag
     4201 ttcattcaag tctgaacccg aagatttgtt tacttttccg aagaagattc tgaggtcctt
     4261 aaaagcatgg gggttgtagg taagaaagtt ttccattcct ttttcgacca tatttttgac
     4321 tgctgcgccg gtgccaaaag gaactctgtc tgagatacgt ggcgaaggat agaccgtagt
     4381 gctgttgaga ctgaaataac ccccggccgg aagcagtttc tggataaaat agaaatcctc
     4441 ccctgcctgc cgcctgttca tgcctccggc ctgcgcatag gcatgagcct ttacagccat
     4501 acatgaacct acagtatgat aaacccaggg atagcctgta tgcaacaagc cctgataata
     4561 atacctcaga tgcagttcat atcttacaac cgcattatat aactcctgcg ggtattcctt
     4621 gccactgagc ggatgttcga aataaatcga acacgccctg cggcctgaat ggttaaacag
     4681 ttccttctcg atagcctgaa aataattttg ctgtaccgta cagtcagcat caagactgac
     4741 tataactccg ttcatcttcc caatggactg aaacctcgaa aaagcctcat ccatgcccgt
     4801 cttgcgtgcc atgccaactc cccatttctt ataggatggc tgtccaatat ccacaacata
     4861 gagtctgaaa aacagagctc cggctttttt acgccaggcc tcgatattac ttatagcaag
     4921 aaggttgttt tcaatcactt cagcagaagc gccgggagga gcattaataa ttataaagac
     4981 ttctgtatgg cattgcggtt gaaagcatga ggccagtgaa tcgagcagtt taataatatc
     5041 tggctcaccg caggcaggta caacaacaat tattccagtt tcagggtgag gaggttcagt
     5101 tgccagtgcc ggaaacagtt ttttatcagc agaccacact tttaaaaggt ctgaaaccta
     5161 ctttttctcc gagcggtact tctcattaag tgcattaagt accttatatg ttatatccaa
     5221 tgtagagtcg ctgtaaagaa caggccctcc gaaagattta ccaagaataa actggtaatt
     5281 atattcgctt ttgttttctt cgagaaactt tgtgatgtaa tcgagtatct gcctgttcat
     5341 aacctgctcc tcttcaatca gttcactctg taatttatct ctcagtgcta ccagatcctg
     5401 ctgctgttgc agcagagcct gttccatctg ggcagcagta gctcttgtaa caagtccttt
     5461 attcactttt tcctgataat ctcttacacc tttttcgtac tggaaagtct ttgaattaag
     5521 ttctgcctca gcattcttct gtttgttgag caggtcattg cgcctgtcgg caaacatttc
     5581 aaaattgaat ataatggtat ctatgttgac ataaacaata ccatgtgact tgctttctcc
     5641 tgctccttca gccgttgcaa ctctttttcc attcccggtt ccggtaaaat gaagtacata
     5701 tatccctgcc actgcaagga aaagtacaat taacagaata gtagacaaat tcttcattac
     5761 aatatcttta ttgttaaaat tttcctgcgc aaatataatt aaataaagag gaaaaagtca
     5821 aatgaaggta aatattcatt aaaattccgg acatggaatt gccctgattc tttttctgcc
     5881 acctgttatt tcgaaggatg tgaattcata cactatcgaa atctcatgcg ctccaccggt
     5941 tgaggatatc aggttcgata ttgtaaaatc ataactgtat ccgatgtgaa gtttatcggt
     6001 tttgatccct atgagtccga ttattgcatc acctgcctgg gatgtcataa atggtatacc
     6061 acggtaccac aaaccgaaaa taaggggact tttataataa tagagcccta tatcagtctg
     6121 gtaatatctc gactgagtct ggaaattcat tgcaactgac agggcttcaa tcattttagt
     6181 tctgagcctg gttgtcccaa ctattcttac gccgccgtaa aggttgaatt ttacaggtaa
     6241 ctgggcttcg tcgccgtaga atgattgttt gggagtaaaa agatgatcga gtgtaaagcc
     6301 tgcccatatt gcatcgttat aaaccagtgc tgaagttgca aaatcgacat ctgccacttt
     6361 gtcgaatggt ggcggtgtta caggcggtgt ggttccggta ccggttatct gactgttaaa
     6421 tattagtttg taaatattca aacccaggta ataaaactta aaatggactc cgggtctaac
     6481 atgccaatca cgtgtgatat taaaatcata agaataaagt aacccaatat ttgtagtgct
     6541 taaatcacca gatccggcaa catcataagt tgctagtacg cctatacctg aactgaaatt
     6601 tggcattgcc ttatcgaatg aaatgctgta tgtttcaaaa acacccggaa ttgcaggcca
     6661 ctgattgcgg taatttattc caagcctgta ttcttcagtg gcaccggcaa atgaaggtga
     6721 aaggtataga ggattggcat aaaactggga gaatgtggga tcctgcccga atgtattgat
     6781 tgccaggaaa ataaaaacta ttagatatat aaatctcttt agcaaaccag gtaacatatt
     6841 gtttgttact cgaaaaatac tcatttagtt ttaataaacc tctgtttttt taacgtattt
     6901 tttccgcaaa aagatacaga aataagaata ttttcaacaa atattcaaaa aatttagata
     6961 aaaaccacga attttttgat gaaatatcat taaagagtga cggaaagaaa catttagttt
     7021 cctctcagga gagtaacatc tcccattttt acataggttt caccgttctt gaatgttact
     7081 cgtaccttcc atacatatac acctggttcg catagattgc ccttgttgta gccatcccat
     7141 ccgatgttta tatcgttgct ctcaaaaatc agtatgccgg ttcttgtgaa tattctgagc
     7201 tgatactcaa ccactccact ggctatcggg tgaaagatgt gtgccgaggc atcactcgtg
     7261 gcactgtaat aaccacccgt tggaccatcc ctgttgggta tgaaagcatt ggggaattct
     7321 atgaaataat ccgaacctcc taaagcattt attacagtta ttgaatcagc gcaaccaaat
     7381 tctgaccata ctgtcagctt tatattatac tttcccgttc tatcgtatct gtgaatgggt
     7441 tcgaaagcaa aggaaactgt tccatctccc agatcccatc tgtacctgac agcatcattg
     7501 gaataattta taaacttaac tgcatcattc cctccggttg gatttaatgg ctcaacctcg
     7561 aagcgtgcag atggtttcgg atgaactgta ataacttctg ttgcatagga gactgtgcct
     7621 ccgccgtaaa cattcagtac aacccggtat gctccaggcc ggtcgtaaat ccattcagga
     7681 ttaaccgata ccgacgaccc accatccccg aaaatccatt tgcatgaatc ataggccacc
     7741 gatgtgttat aaaaagaaac tttcagaggc atgcatccct gcaggaaaga tgctttgaat
     7801 gacgcaacag gagttttgcg aatgtttgca ttggttgttt tatctgaaac aacaggtgaa
     7861 aactccattt tcttcaatga atctgctgcc ataacaccga cactcttatt ttcctgtgga
     7921 ggctgtaccc cacgctttct ttcatcaggt ttcactaccg actgtgaaat tgcctgtttt
     7981 tcagtttttt cttgactttc tttcctgatg actgcaggag aatagaacat tgaaacagta
     8041 tcagacctta tttcagattt aatattaatt gtaccttgtt ctgatttttc tgccctgtcg
     8101 tcatgtgaga ataacagcac tgcagcagca attccagctg caactattcc tgctgcataa
     8161 tatatattga acctaaccgg attaaaacgc aaaaactcct tcactgccag ctttctcata
     8221 aggctatgcc tgactgccgg atcaggtaca gcctcgatgt tatcaagctt gctcctgaaa
     8281 agttcatcca gttccatatg tcttctatcg cccatcactt ctgtaaatct gctatattat
     8341 cctctgcatt gtatttttcc ttaagttttc tgagtacttc cagtttctcc ctcaatgctg
     8401 cttttgccct gctatactgc gatttggatg tatttatgtc aatacccagc atctctgcta
     8461 tctccttatg cttataacct tcaatggcgt acaggttgaa aaccattctg tatcccggtg
     8521 gtaattcatt aagcacttta aaaagttcat ccgatgtgaa aaaatcttct tcaaaactgc
     8581 ttacacctgt ctcaacggaa acatagtcct caatttcaac ataatgttta tgtttaagat
     8641 tcctgtgata gtgagttatt gcagtattga ccacaatttt tcgcatccat cctgtgaaag
     8701 aacctgttcc ggaataatct cttatactga aataaatttt cagaaaactc tcctgtaaaa
     8761 tatcctccgc ctcggctcta tcacttgcat aacgcatgca tacaccaaaa agaaggcgag
     8821 aatacctgtc gtaaagcatc tgttgcgctc tcctgtcatg ccgtgcacat ccttcaatta
     8881 tttcattctc gctcaacata tgctacgaca tttttaagac aatctatgaa ttcgaatggt
     8941 tgcattgttc aaaaaatgtt caaaaagtaa acgaacatct ttataaagta ttttgaatga
     9001 caaacatcat tgaaaatatt tcagatacct tcttactttg ccacaaattt aatctttatc
     9061 agtgtcaggt ttgcactaat attgaatatt tttggaataa agaaaaaaca aaagatatga
     9121 ttaataaatt aattgcatcc acactgccct acttcccgaa gaaatttata tggcttttct
     9181 cgagaacata catatccggt gagactattg atgatgccat gcgtgtatca gctgaactca
     9241 acagacagaa aatcaaggtc acaattgatg ttctcggaga gtttattaag aatctcgatg
     9301 aagcacaaaa aaacaaggag gagtatctta acctgataga tgttactacc cgtcacggca
     9361 ttgacggtaa cttttccctt aaaccaacca gctttggatt gctcattgat aaagagatct
     9421 gttacaaaca catacatgaa atagtagcca aagccgcatc ggtcaacaac tttatcagaa
     9481 tcgacatgga agattcctca tgcaccgatc tggaaattga gttgtaccgc aggctaaaag
     9541 ctgaatttcc gaagagtgtg ggacttgttc tgcaggcgta cctgcacaga acccgcagtg
     9601 acatagagaa actgcttgaa attcatacgc ccgattcacc gctgaatttc agactgtgca
     9661 agggtatcta cattgaacct gaaacaattg cctataagaa atatgaagaa ataaacatgc
     9721 attaccttga ggatctcgaa cttatgctca agaataaaat atatgtaggt attgccaccc
     9781 acgataaacc gctgatagac ggtgcatatg acctgattaa gaaatacgat gtaccccgta
     9841 acatgtatga attccagatg ttgtatggtg taactccgaa actcagggaa aacattgtaa
     9901 atgagggaca cactatgcgg gtttatgttc ctttcggaaa agagtggttt ggttattcaa
     9961 cccgccgcct gaaagagaat ccgaaaatag caagccatat cattaaagca ctcttctgga
    10021 aaggatagtg cacaactgta gcagtagctt gaattacttt ttgttgcgta ttttttccag
    10081 aaagatttta ttcagctcct caagctcttt ttgagatatc tgtggctctg gcggagccgg
    10141 tggttcgggc aaaccatgaa gaataagata agccgcaggt aataaaagca agggacttat
    10201 ccctacaata cggtcggatg gaatccctcc cttctcataa gcttctttct tttgtacctg
    10261 gcgcagaagc tcataattca tttccgggtc tcccagtttg tactgccctg tccttccctg
    10321 aaaagcggca atatttacat tgctgcgcgc atattcggtc ttaacatctg aaggcaccgg
    10381 agaagccatc agttcagtct tcaaagagcg gttgcgcggc aaaattatta cctcaccaat
    10441 gcttatagtg tcgggcttga gataaattcc tgccagaaat tcagttgccc ggagcgtgtc
    10501 actcacaata atcctggcca attggtaacc cattgccctg aaaactactg tgtcgttggg
    10561 ccttgcaaga aacgaaaatg taccatcagt ccctgtaaca gtctttaccg ccccgttcag
    10621 atagatcttc gaatctgcaa tcctggcctg agtttctgca tcaataacca cccccctgaa
    10681 gagtattgac ctgcctgata gttcagactg ctgcgcagac gcaacaaatg tgagattaat
    10741 aagtattata gcaacaaccc accccggtct catataattt tttacaattt gagtatcagt
    10801 tcatcaagtg ttcttttagg aacatggtga acattattct catcacgcca gtatttcaca
    10861 ttgtcacctc ttatatcctc tatcacaacc ttttcaacag gtttaccgag agccagcacc
    10921 agaagaattt cgtactgaag aggaatctgc agttcttcgc gcagtttatc tttctttatt
    10981 gatgctatca tacacccccc aagaccttca ttcacagctc ccagcataat tgtctgtgct
    11041 gcaattcccg ggtcaatccc gaacgactcg gtgatggttg tgtcaccaag aataattatg
    11101 taagcagctg gcctttctcc gggttccggt cccggccaat cgggcaggca tcctgcccac
    11161 gctgtgcatg ggaaaacttt ctcgcaatcc tccggcttat tataaataag atatttcaat
    11221 ggctgtctgt ttgcagccga tgccgacagc cttgcataac caataagctt ttttaataat
    11281 tcataatcaa tcctgaagga ttcatcaaac cttctgtaac tgcgacattt tgatacaagt
    11341 tctgaaaaat ccatatctct ataattttaa tattcaatta gctttttata gtttttgctt
    11401 gtatagttat taattgtcca gctcagcctg ttcgcgggta agaaaaatcc ttgcaggagg
    11461 ttcaggtatt acacctgcct ccgatgccat cgagaatagc ttctcaactg ccctccttcc
    11521 ttcaagaccg agctctgcag taaattcatt aacatacatt tcgatatgac tgtgaagaac
    11581 atctgactcc atttcacgtg catgttgtct tacaaaatca aaggtcgagt agagatctgt
    11641 atatgcatac tctatgcttt tcttgagtat cctgtttact ttcaatgcaa tatcttccgg
    11701 tacagctctg tttattacaa ttgccccaag aggaaccgga tatcccgtaa cctcctccca
    11761 gtaccttccc agatccacta tttttcttaa accctttctg aaataagtaa aacgggtttc
    11821 gtgaattata ataccggcat caacctcgcc gtttagcaat gcattttcaa tttcagaaaa
    11881 aacgtattct tttttgttta atgcctcagg ccattttata ctgaaaagaa gattagcagt
    11941 agtatatttc ccgggaattg caatcaaaag atttttaact tcatcttcgt gaatttctct
    12001 ttttgaaatc agcaaaggac cattacccct tccgattgca cttcccgaat caaggatgag
    12061 atatttatct gcagcataag cataggcatg ataactaatt ttaagtatgt cagtcgttcc
    12121 ctcaaaggcc cttcggttaa gttcctccac gtcagcaaga aaatagtcaa attccagtcc
    12181 ctccgtatca attttccggt tcaccattgc atgaaatacg aaggtgtcgt tcggacatgg
    12241 ggtgaaacca agagtaagct tcataattta ctgtaacaat gtcaaaagtt cttttaattt
    12301 ttctgaaaga ttatttattg ccagggggat attccagttc ttcctctctg cagctcccac
    12361 cctgtttgat atagccctca gcgcaataaa cggaatcttt tccatagaac atacataaaa
    12421 aaaagctgcc ccttccatcg tctcaatgtc ggggttaaaa tgtgccttca gcctttcaat
    12481 tgttttctca gaacctgacg acatattcac tgtaacggct ctgacaggcc tgaaaaagtt
    12541 ctttattctc ccggtaaact cattgtcaca aattatgttt ccgccagaga aaggaaattt
    12601 atcggattcc atcaggtaag tatccttaag gggtacaaaa ccagtgtcgg tctcaatacc
    12661 aaggtcggca aaacagtcag agacaggcat aaccacttct cctatactga tattctcatt
    12721 gaacgtgcct gcaattcctg catttattgc cagatcaggg ttaccgtaat tctggagcca
    12781 tttcttcaat gcccacgctg ttgccacaga accaactcct gtcaccagca cagaaacctc
    12841 atgaggtccg gcacggaaag gttcttctcc ttttccttct tttatgcatt ctgcctcatg
    12901 gtcagtggca aatatcaaca aaatcttcac aataatatta tctttgcagt tcattaaagt
    12961 gaggactaag ttattgtttt taccttgaaa ttgtttaacc ttacatgctt taaaaaataa
    13021 atgggtcaaa aattgtacct tacaagaagg gaaaaattca gtgcagctca caggatgtat
    13081 gttcctgagt ggactgatga acaaaacctg gatgttttcg gaaaatgttc caatcccaac
    13141 tggcatggtc atgattatgt tctctatgtt accgtcagag gagatatttc tggagaaacc
    13201 gggcttgtta tcaatatgaa tgtgctgaaa gaaattatca gggagaagat aattgacaag
    13261 gtagatcacc gcaatcttaa tctggaggtt gattttatga aagggatgaa tgccactacc
    13321 gaaaatctgg cagtagcaat ctggaatgag ctggaggaag ctttaaaaag tgcgggctgc
    13381 gaattatatt gcgtaaaaat tgaagaaaca gaaaacaata ctgtagaata ttatggcaaa
    13441 aaagatcatt aaagtactgg aaaacggtgt agaagaaacc attaaaggtt acattaaaat
    13501 tgagcagtgg aacaaggaga atgttgatga acttgcttca cattattata aggttcttgg
    13561 cttgctgggg gaagatcctg accgcgaagg acttaaaaag actcctgaaa gagttgccaa
    13621 agctctttat ttcatgacat ctgggtacca tcagaaccca tgtaaaattc taaactcggc
    13681 aaaattcaga gaagactaca aacagatggt tctggtaaaa gatatcgata tcttctcgct
    13741 ttgtgagcat catatgctgc ctttctttgg taaggcacac gttgcataca taccgaatgg
    13801 atatattact ggattaagta agattgcacg tgttgttgag gctttcagcc ggaggctaca
    13861 ggttcaggag cggctcacta cccagataag ggactgtatt cagggttgcc tcgagcccct
    13921 tggtgtagca gttgtcattg aagcacagca cctttgcatg atgatcagag gggtacagaa
    13981 acagaactct gttaccacaa catcggcatt tacgggatgt tttctcgaca atttccggac
    14041 aagagaagaa tttatgcacc tgataggaac aaaactgcat tgaactttca acatttaact
    14101 aaaaaacaat caaaaacaag tcaataaatc agtttatacc atttatgaaa gcatacgttt
    14161 tccccggtca gggttcgcag tacccaggta tgggtaagga cctgtatgaa aaatatccgc
    14221 ttgcaaagga gttgttcgat aaagccaatg aaatactcgg tttcagaatc accgacctga
    14281 tgttcaatgg aacagaggaa gacctcaagc agacgaaggt aacgcaacct gccattttcc
    14341 tgcattctac aataatcatc aaggtgatgg gagatgcttt ttcacctgat atggtagcgg
    14401 ggcattccct tggtgagttt tcggcactgg ttgccaaccg taccatatcg tttgaagagg
    14461 gactggttct tgtttcaaaa cgggctgctg ccatgcagaa agcctgtgaa atgaacccat
    14521 caacaatggc tgcaatactt ggactcgacg actccgttgt ggaagaagta tgcgcagggt
    14581 ttaacgggct tgttgtaccg gcaaactata acagtccggg gcaaatagtc atctccggca
    14641 cctttgaggc aattgatcag gctattgaaa tacttaaggc aaagggtgca aaaagagctt
    14701 tgaaacttca ggtgggcggt gcattccatt ctccccttat ggaaccggca agaaaagaac
    14761 ttgaagaagc aataaacagt acccccttca aaaagcctgt atgccctgtt tatcagaatg
    14821 tcaatgcaaa accatcaacc gatccgggag aaataaaagc caacctggtg atgcagctta
    14881 cttcaccggt gagatggacc cagacaataa taaacatgat caacgatggg gcaacatctt
    14941 ttactgaagc aggacctggc aatgttctgc agggattgat aaaaaaaatc aacagggata
    15001 ttgaagttgc aggggctgtg gtatgaatat aaaagctcct gatttatttc attaattgct
    15061 ggaaggtttc attaccacac ctttgtgttt tctgccgtca ataagtattt caagaggttt
    15121 ttcaaacctt acatggcgga taaactgatt ctcataaaca acctgttgct gattgagcca
    15181 gtcaacatca aaatgcccgt cgccgttgta aggatttact gttatatatc ccactctgaa
    15241 tgatgtaaga ttctggaaaa agtgggtgcc ctggctgggg tcgattctgt aatcttttag
    15301 gcctgcctca acaattaccc gtgcggcaga tatctgaggc catttcacag gtatcccgag
    15361 ccatgggtca ttcgaacccc aacgacccgg accaatcagc acataaccct caccattacg
    15421 ttgaaaagag gtattgattt tttcaatatc aactgcaatc ttttcactgt aagccggatt
    15481 gaatgtctcg ggcttcagat aaactatatc tcttatatcc ttgaaaacac cattaccaag
    15541 agccgactcg gaatatatga tagtatcctc agggttgacc ttatcaatat caactttata
    15601 aagttgttca ttaagaacta tgggtctgat ctgcagaaaa taaaatattt taggtgttcc
    15661 gggcggtgtt tccaggttgg cagcaaattc tatctcaatc ggatgattca tttcctctga
    15721 gcctatttcg agaagagtgt taagtatttc cgcaagcgga aactgattgt attgtaaaat
    15781 actggcaaaa gttatgatac gtacgcccgg acgtccgata ccgtcataaa gaatattatt
    15841 gttccggtcg taggtcgaag ccagatacct catcgccgtt tcattattta catcgcttat
    15901 cttaacttta agcaggttaa cgccgtcatc ggttgaagga acgaaacggt ttacgttcag
    15961 gtcgagagca tagaaaaact tttgtgtatc tctcaatgcc atctcagggg aactaagctg
    16021 cagtattttc ttcgggtgct tgggcgaaaa acgcaatgtc tggccgcctt caacaaccag
    16081 ccgacccagg cctaatgcca catttgcaat gccatcttca gccttttcag agccaatggg
    16141 gtaatagtta agtgaccttg ctacccccga aaatgttgga tagaacattg tctcatgcct
    16201 tgtgccgcaa acttcctgta ttattatacc catcttttct tcatctatca cattggaagt
    16261 agcagccata tatgctttgc tcatcctgta atacaccgaa gcatatacct cctttatggc
    16321 atcagcaagc agctgcacca tataacggtt gtcgggaacc cggggtatca tataagttga
    16381 gtatattccc gcaaacgact gataatatga atcttccagt ttactggaag atctcactgc
    16441 aagcggatag ttccttgaaa ctgtcagaaa agcgtataaa tcctggtaca gccatcccgg
    16501 caattcggcg cttataaagc gagcaagaat atcttcatcg gggaggtccg acatagcaat
    16561 ggagtaaagg ttgttggtct ccataaactc gtcgaacaca tcagtactaa gcacaatagt
    16621 gcgggggatg gttattatta cgtcaggaaa tttgttataa agtttattct tcttgataat
    16681 actgtttatg aacgcaagtc ctcttgcctt tccgccgata gaaccctctc caatgcgtga
    16741 aaagatctgg tattcatcaa aattagacct gtcgaatttt gcaattactc ctcttccctt
    16801 tcctaggcgg aaactcgata tggcaacata aaggaaccgg cgcatttcat ccagagtctc
    16861 aaaatcttcc ttgcgaagat atttgaacaa ctgggctacg gggaagagtg ctctggcatt
    16921 cagccacttt gaaaaatgat tacggctggc gtgatattct agcgatttgt cgggaactgt
    16981 gagtattttc tgctgaaggc tctggaggtc ggtcgcgatg gctattggct ccatcgtgtc
    17041 aggattcctg aaaacaaacg gcccgaatgc aaggttctgt atcagataat tcctgagttc
    17101 aagtgacagt gatttggaat atttgtatat aaatcctgca cccagctcct cggcaacttt
    17161 tttgtgttca aggcttgacg actgcaaaag gaacggcacc tgatcgtcgt cggcctttac
    17221 cattttgcaa agttcaatac ctgcattttc atcaatctcc ccattacgct tataactaat
    17281 atctgaaata attcccagta cgttgtactt atatttctgg tatagctcta cggcttcctc
    17341 ataattggtg gctaaaagta ttttgggtct tcccctcatt ttcagcatac gctgatgctc
    17401 attaagagcc tctcgttgaa aatcaagtga ttgagcaagt attattttat aaagactggg
    17461 aagatatgac gatatataac ggcgtgagtt ttcaaccagt attattgcct gaacgccaac
    17521 ctgttcaata tcatggtcgg cattcattct gtcttccagc agttttatga tcgcaagtat
    17581 taatgatgca tcgcccagcc agcagaatac ataatcaatt gcactcaggt cttctttttc
    17641 gagccttact gaaacttccc tcgaaaaata tgtcagaacc acaatcggaa ttttaggata
    17701 aatgctcttg atctgctttg ccagagcaaa aacatctgtt cctttaacgc taagcatcgc
    17761 tataaccagg tctatattcc cctgggcaag tattttgaaa gcatcctcta cattgtcggt
    17821 ttgtataaaa gtaggtggat atctgaggtt cagcgataca tattcattaa aaatctgttc
    17881 ctcgattctc ccgtcctctt caagcatgta attatcgtag ttactgcaga taatcagcac
    17941 cctgtgaatt cttttctgca tcagcaggtc aaaagaagta tctgtaaaat catacttctc
    18001 catttccagt attccgtcaa tcactgccat ccttcaaaga ttggttaaaa tctcttcagt
    18061 taaagatata gttatataat gctatcttct taagatagag atgtaaaatt actaaatatg
    18121 ttgatatgat taaacttccg gtgatgtttc aattaatagc gggaagtaaa gtggttttca
    18181 ttccacaaca aagacagttc ccgattcaag gaggtcgtca acacatttaa tttctgactt
    18241 ttcttttacc ctttttatct gttcagcaac agccgcttcg tatgtctcgc cctccaccga
    18301 tctgattaca cccagtgcta caggatattc aggatatctc atatttgcca gaaaataatg
    18361 gatcccgggg ttgggttcat gggcgttatg caccaggata tctttctcct ttatcccttt
    18421 ctctccaata cgtaccactt taagtttaag cccttcgagg atgagtccgc tttttctatc
    18481 ctttccgaac agcatcggtt ctccatgtct cagaataatt gttcggtcat ctcttacatc
    18541 tttaccggcg acaacatcat gtattccgtc gttgaaaatt acgcagttct gaagcacttc
    18601 aaccactgaa gtgcctttat gtctggctgc ctcgacaagt atttcagttg taaggttaat
    18661 attgttatca actgttcttg cgaagaattt accttttgct ccgattacca gctcacccac
    18721 tgagaatggt tcttccaccg ttccgaatgg tgaagttttt gttaccagcc ctttattgga
    18781 ggtgggagag tattgtcctt ttgtcaggcc gtatatttcg ttgttgaaga gaatgatatt
    18841 aatatcaata tttcttctga ttgcatgtat aaaatggtta cctcctatcg ccagagaatc
    18901 gccatcgcct gttatcaccc atactgaaag atcaggattt acagttttta ctcctgtagc
    18961 aatcgctgca gcctggccat gaataccatg aaatccgtag gtgttcatat aataaggaag
    19021 tctcgacgag catcctatac ccgaaacaaa aacatacttc tcctttggaa caccaagttc
    19081 tgaaagtgcc ttctggattg aggcaagaat tgcatgatta ccacaacccg ggcaccatct
    19141 gacttcctgg tcgctcttga aatccttcgg gctgtacttc agatcacttt tatcttccat
    19201 tttatttaat ctccagaagc tttatacagt tttctttgat ttccttaacc gtaaatggca
    19261 aaccctgcac cttgttaatc tgataaaaat gaaactgttg aaaattcatt ctgaggtagt
    19321 ttgcaaactg tccaaggttt atttcgcaca cagcaatttt tttgaattta ctgaatacat
    19381 ctcttacatt tgacggcagg gggtaaatat aattaaagca agcaaggctt acattatatc
    19441 cctcttcacg aagttctttt acagaagtta gcatatgtcc gtaagtgctg ccccagccaa
    19501 ccaggaggag gtcacctgtt tcgtcgccgt aaacattcag aggaggaatt ttttcagcca
    19561 ctcttttcac tttctcatct ctcatcttaa ccatatattc gtgattctca ggaacataag
    19621 acaccgttcc cttaattgtc ttttccagtc cgcctattct gtgttccatt cctggggtgc
    19681 ctggtattgc ccagtaacgg ttcaggcgtt tttcatcgcg tttataaggg atatatggtt
    19741 cgttctcttt tcgtgcgtaa ggtggtttga tatcggggag ctctgacatt ttcgggatat
    19801 gccatggttc ggagccattt gccagatagc tgtcggaaag gagcaggact ggtgtcatgt
    19861 gttcgatggc aattttcgct gcctggtagg cataatgaaa gcagtcggta ggtgtgctgg
    19921 cagcaagtac cactaccggg cattctccgt tacgtccgaa taaggcctgc ataagatctg
    19981 cctgttcagt ttttgtaggt aatcccgtgg aaggccctcc cctctgaaca tcaacaataa
    20041 caagtggtaa ttctgccata actgccaggc ctattccctc tgtctttaag gcaagtcctg
    20101 gccccgatgt tgaggtcact gccaggcatc cagcaaaact ggcacctata gccgagcata
    20161 ttcctgcaat ctcatcctcc ccctgaaagc taatgactcc caaatcttta
//
LOCUS       DAOSNB010000011        19284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2842_length_19284_cov_4.379011, whole genome shotgun sequence.
ACCESSION   DAOSNB010000011 DAOSNB010000000
VERSION     DAOSNB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_19284_cov_4.379011"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLG42_01190"
     CDS             <1..395
                     /locus_tag="PLG42_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU29854.1"
                     /translation="TGNPLKQDIVEDIKRVEPELLDVVFECCGKQEAADQAVKLLKPG
                     GMLVIAGIPSFSEWKFSAEDIRRKEITIRNVRRQNEMVQPTLDILSAGKINPEKMITH
                     TFDLDHVSDAFELVAGYRDGVMKAMIHL"
     gene            complement(401..1321)
                     /locus_tag="PLG42_01195"
     CDS             complement(401..1321)
                     /locus_tag="PLG42_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOU29855.1"
                     /translation="MNKKTNTYFLAILTCLLWSTVYASIKIGLKYEAPLYFASKRFII
                     AGVILLPFAGKLRDYISVIRDHTKLLLQVTVLQVIMNYLLFYLGMNLVPGALGAIIVG
                     SQPLITAIVAAVLTDNNTVTRKKMFTIVSGLAGIVLINAGRQVLRLGSATELLGVILI
                     LGANISTASSNVLVSVNNKNVNPVILNSISLLAGGIIIFFLALILEKPAEEILPASYW
                     MSLLWLSFVAAFAFSTWYKLLQRPEVRVSELNLWKFLIPVFGAIISWIVIPEEKPDIM
                     TVGGIVIISTSLLLFFSGIKGITFDRAINK"
     gene            complement(1360..3624)
                     /locus_tag="PLG42_01200"
     CDS             complement(1360..3624)
                     /locus_tag="PLG42_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOU29856.1"
                     /translation="MFSKRINCFRITVLSILFTIIQHSYSQPDTSDYGRADRLQQMVS
                     GKVFYGNVRPTWINNTGRFLYESTTPEGTEYYIVDAISKTKRVAFDQKKFAEAFREAT
                     GQKVEPGKLPIRNLVFSERMMSFSFNYDNYNWICILRNYRIIRGDRTVERARPQDAWS
                     WGFRDELQNAPVQSPDKKWTAYIKNYNVYVKSNDDNKEYQLSYDGGTGEYYSSYIIWS
                     PDSKKLVAKRVRPAEKHMIHYIESSPEDQLQPRHFSYEYQKPGDAVPQFYPQLFDVEN
                     KKHIKLDDSLIPNQYYIDEIKWSKDSRYFTYEYNKRGHQVYQVIKVDATTGECKVIIN
                     ETSQTFIHYSGKRYRYDLEKTNEIIWASERDGWNHLYLYDSETGQVKNQITRGEWVIR
                     GVIGVDEEKRQIIFQASGKEPGDPYFIHVYRINLDGTGLKRLTDGYGNHEVSFSPDKR
                     FFVDTWSTVDTPPVAVLRNGEDGSLIMELEKADITKLLETGIRLPEPFVAKGRDGVTD
                     IYGIIVRPMNFDPSKKYPVIENIYAGPHSSFVPKSFRPIISGMHQLAELGFIVVQIDG
                     MGTSNRSKAFHDVCWKNLKDAGLPDRILWIKAAARKYPYMDTTRVGIYGTSAGGQNAA
                     AAVLFHPEFYKVAVAACGCHDNRMDKMWWNEQFMGWPVGPEYAESSNVENAWRLQGKL
                     MLINSEMDKNVDPSSTVQFVDALIKAGKEFEYVIIPGAGHSSGGPYGERKRRDFFVKH
                     LLGVNPPDWNNTSN"
     gene            complement(3822..5654)
                     /locus_tag="PLG42_01205"
     CDS             complement(3822..5654)
                     /locus_tag="PLG42_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOU29857.1"
                     /translation="MKKFLTATIFYSFFWLAFFFFARVIFLLMQFKEMVRYSFADIIQ
                     TFLHGFKMDLSVTAYILILPLFLGAFWFVFAGKWYQKFMKIYSAFLIIFFTLLVLGDA
                     VVYSYWGYRVDFSVVAYLRDPKDAFASASTLQLIGGVSVSALLIYLQLKGLKWFIKRF
                     YSFVPEKRFVLLKTSLVIILTGLLIVPVRGGFGVAPLNAGHVYFSEHLFLNHAALNEV
                     WHFGHSTAYLKPLKNPYEFTSKEEALGNFKNLMLDEGKTTMLLNTQRPNILLIIVESF
                     GSEIISAREGDSVVAPRFNSFIPEGIYFSNFYATGSRTDKAIPAILGGYPNLPTIQVI
                     REPKKTQSMPGIFKMLDSAGYKTSFWYGGNINFANINSFITTTGFKEKVTIDNFERKY
                     RNSKWGAHDHVVFERLYDSLLSVKEPFAYALLTLSSHEPFEVPMKPVFKGNSILSRFK
                     NSIYYTDKSLGEFLDRAKNTEWWKRTLVIIIADHCRRNSETIPAYSETIFRIPMLWLG
                     GAIAVKDTVISKPCSQFDLPATLSAQLNLPDVFPFSKNILSDGVKDFAFYTYNEGFAF
                     ITDSSKVIYDIRLNGPVEKSGNWEIAEKYGKSFLQVLFDDYLSR"
     gene            5769..7370
                     /locus_tag="PLG42_01210"
     CDS             5769..7370
                     /locus_tag="PLG42_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOU29858.1"
                     /translation="MTVAEYIAERLFSEGVRYVFGVPGGASIPLIEAIRQAGIAFVLT
                     AEERSAGFMADVTARITGIPGVCHSTFGPGATNMSTGVGCALLDRSPLLALTSEMPDD
                     WIDRTTQMNINHQALFKPVTKATFRLSPRNVQSVLPRALSICNEEYPGPVHLGIPSDI
                     AGNQVSSDVKSQEVRRQQIPENNHDITEIKNLLKSSKNPVIAIGLTAARLCNSRQVNT
                     LLTGFQVPVLLTPMAKGLIDEEHPCYAGVLSHALSDYLEDIISQADLVIGLGYDPVEY
                     NYESWMPDVPLIHFDTVLQDLPRSRDVYSFVGDPTDWFAILSELKLTSGIISSGLLAE
                     VREEMDSVFRGFTSGFGPVTVIKVLGEMLPRDTIATVDVGSHLHLAGQYWKTHGFQNL
                     IMTNGWSSMGFGLPAAIAAKLNKPSSTVVCLTGDGGFLMSAGEVLTARRCLAPIIIVV
                     FSDGELNLIKLKQTWKGLQPYGIHISSGDLFGSEYFLGAKVFNATSETTLRDAVMNAL
                     ILDETVIINAIIDPEDYKWLVVKQK"
     gene            complement(7457..7963)
                     /locus_tag="PLG42_01215"
     CDS             complement(7457..7963)
                     /locus_tag="PLG42_01215"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOU29859.1"
                     /translation="MGKGDRKSRRGKIFLGTYGVRRPRKKKSKKPALQPEIVKVEEAV
                     KKPVREKKKEAVEVVEPAVAVEAVTQEVIEKIEEKAEVTEQVTVEKEVEKKAPKAKKE
                     AAAKKEPVASKETAAKKESGVKKETAEKKTAKEKKEPAAKDTKKKSEEGEKKEKKASS
                     SGKKTGKE"
     gene            8174..8476
                     /locus_tag="PLG42_01220"
     CDS             8174..8476
                     /locus_tag="PLG42_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU29860.1"
                     /translation="MNIYVGSLNFKMQESELKEIFEEYGEVTSARIITDKYSGRSKGF
                     GFVEMPDEADARRAIKELNGAEVGGRTIIVNESIERPERRNFRNGNNDRRRRDSYR"
     gene            8488..8694
                     /locus_tag="PLG42_01225"
     CDS             8488..8694
                     /locus_tag="PLG42_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU29861.1"
                     /translation="MNKMKGNVKWYDSVKGYGFIQTEDNKDIFVHRTGIEGSYRKLET
                     GEAVEFEIEEREKGPVAINVRKSV"
     gene            8883..10178
                     /locus_tag="PLG42_01230"
     CDS             8883..10178
                     /locus_tag="PLG42_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU29862.1"
                     /translation="MLKIFTRCNSPRLKYVADLIFFSILGVPYEIVTDRRRIGRNPVI
                     NYSGEKIDDAFRISPSGLLGETGIKPFDIEVSEWKNLPIFFRTETDADFPFDIFSAIF
                     YMVTRYEEYLPFKPDTHGRFPASMSLAFRNGFLKKPVVNLWIKELAAELVIHFPELVF
                     KKNSFKSFVTFDVDEPFKYLGKDLFRNFGRMLLDLGKKNFSSAERYRTIKGKLKDPWN
                     IFDYLIEKTENSGSDILIFVPTGDRSEFDRWPTWNNEDYAELLAAIRSKTRIGLHPSY
                     FSSDNIKKLKTEKARLESLMASEIFSARYHYIRLKLPVSYSNLEEAGIREDYSMGFAD
                     EPGFRAGIATPFYFYDLNKENKTNLMVYPFQVMDATLVQYRKMNPGDAQCLIEDLIDE
                     TRLAGGIFQSVWHNNILAEGDAAMEWRAVFENTLKYQNR"
     gene            10175..11080
                     /locus_tag="PLG42_01235"
     CDS             10175..11080
                     /locus_tag="PLG42_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29863.1"
                     /translation="MIIYLKNKEIDRNLWDTCLKNSRCLKPYAYSWYLDIMAPGWEAL
                     IDDEYDSVFPVPGFEKFGIKYITTPIFLQQLGAFSPDKPAEKVIHEFIFYMPEFYRFV
                     DLCVNYRVDNKDYKITERTNYVLHLNRDFNEIWNSYSSDCRRNITLASQNSINYTTDI
                     KPKEIINLFIENTGKNIPGIKQRDYERLETLMNHCLFNRKGRIIGVRSGDGRLIFGIF
                     LVEIPGSKTILFTANSSESREKRINYHAVNELIKENASTGIVLDFAGSSIPSVASFMQ
                     SFGSSKETYYRLYRNSLPWPLRMLK"
     gene            complement(11176..11886)
                     /locus_tag="PLG42_01240"
     CDS             complement(11176..11886)
                     /locus_tag="PLG42_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU29864.1"
                     /translation="MEEAHSLDTIIEFRNCNIWQQDHLVLTDVNFSVKRNEFMYLIGR
                     VGSGKTSLIKTINAQLPLKEGEAFVAGYNLKKLRRKDIPYLRRRLGIVFQDFQLLTDR
                     NVHDNLEFVLRATGWKNKNDIETRIAEVLEKVGLGLKGYKMTHQLSGGEQQRVVIGRA
                     LLNDPDIILADEPTGNLDNETSDGILRLLMEINQTGRAIIVATHNQNLLKRYPARTLK
                     CEDGKLLEVDQEENIELI"
     gene            12115..15135
                     /locus_tag="PLG42_01245"
     CDS             12115..15135
                     /locus_tag="PLG42_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU29865.1"
                     /translation="MNKRLLLILFLVLPAVQLAAQISYMNKEVSSEFQKGLELYNKEK
                     YTAALKFFDSWLKNSQGDNANEKFDAEFYSAMSALKLFNPDGEYRMNRFIASWPGSSR
                     INDAYLALGDYFYQNKNYRKAATYYQRVNRILLPSEKLHEYCFKYGYSQFMNGNKEIA
                     LLMFSEIKDIDTYYTSPALYYFSHIAYEEKKYETAMEGFMRLRNDETFGSVVPFYIVQ
                     VLYLRKDYDGILEMAPELLKSAGKMRAVELYRFIGDAYYNKGNYKEAAGYLEKYAEGA
                     KASDRPDRFQLGYCYYMTGDYDKAIKIFLDLTARSDILTQNVWYILGDCYLKKGDKQR
                     AQFAFGQASQLNFDPKIKEDALFNYAKLLYETSYSPFGEAIRAFQEYINLYPGSDRIE
                     EVYNFLVATYMQIKNYREALASLDKITRKDARLEAAYQRVAFYRGLELFKNMELTTAI
                     NMFDKSLDYGKYNNQLRARAIYWRGEAWYRLGEYDNAIADYQTFMGLPGSAQLDEYRL
                     VRYNLGYALFNKKDYTNALAHFRNFESNATAVRADILVDARNRIADCYFINTNYADAV
                     EFYNKVIEFGKIDADYALYQKGFALGLMNNQKGKAETLTILINNYPSSKYIPGALFER
                     GRAWVSLKDKARGEADFNQVISTFPGSPFAPRAIIQLGLLYFDTGENEKAIAQFKRVI
                     ENYSSTPEARYALTGLKNAYVEINDVDSYFAYVKSLKGFGDVAQTEKDSLLYVSGERL
                     YMSGNCDRASEVFRNYLNEFTYGSFRINALFYLAECEMLKGRKDEALDNYQKVIESPG
                     SEFYEPALRSAAALLFEKEDYLKAFGLYEQLERITDNSESQVIALRGQLRAAYEAGDA
                     EKTIRTAEKINKAQNVPEELLREANFMSAKAHYSLNHYDEALRDFRKTAVEITSAQGA
                     ESKYRVAEILVLKGNIAEAEKVINEFINSTTPHQYWMAKMFLLLADVSLKKGDTIMAK
                     ATLQGLKESYSVPDDGILDEVKAKLDSLNAGQIQ"
     gene            15163..16908
                     /locus_tag="PLG42_01250"
     CDS             15163..16908
                     /locus_tag="PLG42_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29866.1"
                     /translation="MKMKINIMKPLMIITAFLAFSALFPARGQETVIKREITLYNPYK
                     PSLPDVVKKSFLPDMTDTSAVKPVIKYEIRTTPYTPPYTVSPLKAATMVPDPLTKLYN
                     GYINMGLGNYLTPLAEISITNLRSKQSGVGIYAGHFSTNGKVKLENGKKEFAGYMDNN
                     VSLYGRKFLDESVLKGSIDFSRKTRYAYGYNPGITDYDPEKKDFRLNYTSAGINAGIS
                     SVRPDSSKLVYEANLDYNFFISEKNMYLHTVKLTGQAGKEVEGFYAGSGAGFEFYKPS
                     GYISSSSAYIAEITPFITRKSADWNAKLGAGFALDKFIDDDDPKLHIYPDLRFGFNIM
                     PSYLAFYAELKGKLERNTPENVIMLNPFMLSDYLYKIRNTCYPLIVSAGLEGESGIEG
                     RYRLNVSYTIADDFLLFTNYAGTRNDTINILMGNYFKPLFDEAEILKFHGETAGRINR
                     HFTFSAEGNYYKYTLTSNKYAWGMPDWDLKFNLKYNLRDKIIASIGVNTTGPRKLLTT
                     TDNFDNLFNLLGSSEKEYSMPAHLNFNFSAEYRYTKILSFWLKFNNLSFTKYYEWAWY
                     PSLRFICMAGFTYSL"
     gene            17245..19215
                     /gene="gyrB"
                     /locus_tag="PLG42_01255"
     CDS             17245..19215
                     /gene="gyrB"
                     /locus_tag="PLG42_01255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOU29867.1"
                     /translation="MSDKLKKENNGNGYSADSIQVLEGLEAVRKRPAMYIGDTGVKGL
                     HHLVYEVIDNSIDEALAGYCDRIDVVIHEDGSVSVTDNGRGIPTDIHEKEQKSALEVV
                     MTILHAGGKFDKDTYKVSGGLHGVGVSVVNALSEWLEAIIHRDGKVFYQKYERGKPVT
                     DVKIIDKTDKTGTIVRFKPDIQIFQTIDFNFEILSARLRELSFLNKGVMLTIKDLREP
                     VENGNGNKIRYEEFISQNGLIEFVKYLDSNREPLIEKPIYISTDKYGFPVEVAMQYNT
                     SFTENVYSYVNNINTIEGGTHLAGFRKGLTRTLKKYAEESGATAKLKFDINGDDFREG
                     LTAVISVKVPEPQFEGQTKMKLGNTEVMGAVDQAVGNALANYLEENPRDARQIVQKVI
                     LAATARHAARKARELVQRKNLLSGSGLPGKLADCADRDPSKCELFIVEGDSAGGTAKQ
                     GRDRRFQAILPLRGKILNVEKAMQHKIFESEEIRNIFTALGLSIGTEEDSKALNTSGL
                     RYHKIIIMTDADVDGSHITTLILTFFFRYMQDLIKNGNIFIASPPLYLVKKGKIEKYC
                     WTEEEREAAVKELGQGKDSSVSVQRYKGLGEMNAEQLWQTTMNPETRILKQVTIESAA
                     EADHIFSMLMGEEVPPRREFIETHAKYARIDA"
BASE COUNT         5910 a         4013 c         3885 g         5476 t
ORIGIN      
        1 ggaccggcaa tccgcttaaa caggatatag tcgaagacat taaacgcgta gaacctgagt
       61 tgctcgatgt tgtctttgaa tgttgcggaa agcaggaagc cgccgaccag gcagttaaac
      121 ttctgaagcc gggtgggatg ctggttatag caggaatacc atcctttagc gagtggaaat
      181 tcagtgcgga agatataaga aggaaagaga taacaatcag aaatgtaagg cggcagaatg
      241 aaatggtcca gccaacactt gatattttat ctgccgggaa aataaatcct gaaaagatga
      301 taacccatac gtttgacctc gaccatgttt cagatgcttt tgaactggta gccggataca
      361 gggatggcgt gatgaaggca atgatacacc tgtagctatt ttatttattg atcgcgcggt
      421 cgaacgtaat tccttttatt ccgctgaaga acagcaggag agaggttgag ataataacta
      481 taccccctac cgtcattata tcaggcttct cttcaggtat tactatccag ctgattattg
      541 caccaaaaac aggaataagg aatttccaga ggttaagttc cgacaccctg acttccggcc
      601 tttgaagaag tttgtaccag gtgctgaaag caaatgctgc cacaaagctt aaccatagca
      661 gcgacatcca gtatgaagcc gggagaattt cttctgccgg cttttcaaga ataagtgcca
      721 ggaaaaatat aattatcccg cctgcgagca gagatataga atttaaaatt acagggttga
      781 catttttatt atttaccgat acaagtacat tactcgacgc agtggagata tttgctccga
      841 gaatcagtat tactcccagc agttctgttg cagaccccag tcgcaaaacc tgccttcctg
      901 catttattag cacaattcct gcgagccccg aaacaatagt aaacattttt ttccgggtta
      961 ctgtattgtt atcagttaaa acagctgcca caattgctgt aattaaaggc tgcgagccta
     1021 caattattgc tccaagtgca ccaggtacaa gattcatccc caggtaaaag agcagatagt
     1081 tcattatcac ctgaagcaca gtaacctgaa gaagaagttt tgtgtggtct ctgatgactg
     1141 agatataatc tctgagcttc cctgcaaacg gcaacaaaat aacccctgca attataaacc
     1201 tcttcgatgc aaaataaaga ggggcttcat acttcagtcc tatttttatt gaagcatata
     1261 ctgttgacca cagcagacag gtaagtatgg caagaaaata agtgtttgtc tttttgttca
     1321 attcccgaat aattttaagc ggcaaatatc cgaaaaaaat caattcgaag tgttgttcca
     1381 gtcgggtggg ttaacgccca gaagatgttt tacgaagaaa tcacgtcttt ttctttctcc
     1441 gtaaggtccg ccgctggagt gcccggcacc cgggataata acatattcga attccttacc
     1501 tgcttttatc agtgcatcca caaactgaac cgtggagctg ggatccacat ttttatccat
     1561 ttccgaattt ataagcatga gctttccctg aagcctccat gcattttcga catttgacga
     1621 ctcagcatat tccggtccca caggccatcc cataaactgc tcgttccacc acatcttatc
     1681 cattctgtta tcatgacacc cgcacgctgc aacagccacc ttgtaaaatt caggatggaa
     1741 gaggacagct gctgcagcat tctgaccgcc agccgaagtt ccatagatgc ctacacgggt
     1801 ggtgtccata taaggatact tgcgtgcagc agcctttatc cacagaatcc tgtcaggcaa
     1861 ccctgcatct ttcagattct tccagcatac atcatgaaaa gctttcgaac ggttcgaagt
     1921 acccataccg tctatctgga caacaatgaa ccccagctca gccagctgat gcatgccact
     1981 tattatcggc ctgaaactct tgggaacaaa actgctgtga ggccctgcat aaatgttttc
     2041 aataaccggg tatttttttg aggggtcaaa attcatcgga cgcacaatta tgccgtaaat
     2101 gtctgtcaca ccgtccctcc cttttgccac aaaaggttct ggcaacctga tacctgtctc
     2161 aagaagtttc gtaatatcgg ctttttcgag ttccataatt aaacttccat cctctccgtt
     2221 tctcaaaacg gcaaccggcg gtgtatctac cgttgaccat gtgtcaacaa aaaatctttt
     2281 gtcgggagaa aaacttactt catgattgcc ataaccgtca gtcaacctct tcagccccgt
     2341 gccatcaaga ttaatcctgt aaacatgtat aaaataagga tcgcccggtt ctttgccact
     2401 cgcctgaaaa attatctgcc tcttttcctc atccactcca ataacgcctc taataaccca
     2461 ctccccgcgg gtaatctgat tttttacctg acctgtttct gaatcgtaaa gataaagatg
     2521 attccatccg tccctctccg aagcccagat tatctcattc gtcttttcca gatcataacg
     2581 gtaccttttt ccactgtaat gaatgaaagt ctgggaggtt tcgtttataa ttaccttaca
     2641 ttctcctgta gttgcatcaa ccttaatcac ctgataaacc tgatgccctc ttttgttgta
     2701 ttcataagtg aagtacctgg aatccttact ccattttatt tcgtcaatgt aatactggtt
     2761 gggaatgaga gagtcgtcaa gttttatgtg ttttttgttt tcaacgtcaa acaactgcgg
     2821 ataaaactga ggtacggcat caccgggttt ctggtattcg tacgaaaaat gccggggttg
     2881 cagctgatcc tctggagagg attcgatgta atgaatcatg tgtttctcgg caggccgcac
     2941 tcttttggct accagttttt tagaatcagg cgaccatatt atataagacg agtagtattc
     3001 acctgtcccg ccatcgtaac tcaactgata ttccttgtta tcatcgtttg actttacata
     3061 aacattgtaa ttcttaatat aagctgtcca ttttttatcg ggcgactgca ccggcgcatt
     3121 ctgaagctca tccctgaaac cccagctcca tgcatcctgt ggacgtgctc tttcaacagt
     3181 tctgtctccc cttatgattc tgtaattcct taaaatgcat atccagttgt aattatcata
     3241 attgaatgaa aaactcatca tcctttccga gaaaaccaga tttcttattg gcagcttacc
     3301 aggttcaact ttttgccctg ttgcctccct gaacgcctct gcaaactttt tctggtcgaa
     3361 ggctaccctc tttgtctttg atattgcatc aacaatgtag tattcagtac cttcaggagt
     3421 ggtactctca tacaaaaacc ttccggtatt gtttatccat gtagggcgca cattgccata
     3481 aaacactttc cctgatacca tctgctgaag acggtcggct cttccgtaat cagatgtgtc
     3541 gggttgtgaa tatgaatgct gtatgatggt gaaaagaata gacaggactg ttatccggaa
     3601 acaatttatt ctcttactga acatatcttc tcttctttat ggaatttttc aaagataacg
     3661 taactggaaa taataagttg ttaaaaatct tcattaaaac tccattttaa ttttttttaa
     3721 gactacacaa tactatcctc attaaaaata aattaaagcg atgattcatt caccagcatt
     3781 tactatatcg ctgaaaaagg ttaaccaatc tacataatta cctaccttga gagatagtca
     3841 tcgaaaagca cctgcagaaa tgatttacca tacttttcgg caatttccca gttgcccgac
     3901 ttttctacag ggccgttaag cctgatatca tatataacct tagaactgtc ggttataaag
     3961 gcaaaacctt cattgtatgt gtaaaatgca aaatctttta cgccgtctga tagaatattt
     4021 ttgctgaacg gaaaaacatc gggtaaatta agttgtgctg ataatgtagc agggagatca
     4081 aactggctgc atggcttact tataaccgtg tccttcacag caatagctcc accaagccag
     4141 agcataggta ttctgaaaat tgtttcagaa tatgccggaa ttgtttccga gtttcttctg
     4201 caatgatctg ctattataat gacaagagtt cttttccacc attctgtatt ttttgccctg
     4261 tctagaaatt ctccaagaga tttatcggta tagtatattg aattcttaaa tctgctgagt
     4321 atactgtttc ccttaaaaac gggtttcatc ggaacttcaa atggttcatg actcgacaaa
     4381 gtgagcaaag catatgcaaa gggttccttt acagaaagaa gtgagtcgta gaggcgttca
     4441 aatacaacat ggtcgtgcgc accccatttt gagttccggt atttcctttc gaaattatca
     4501 atggttacct tttctttaaa ccctgttgta gttataaatg aattgatatt tgcgaagttg
     4561 atgttgccgc cataccagaa agatgttttg taaccggcac tgtcgagcat tttgaaaatt
     4621 cctggcatcg actgtgtttt tttaggctcg cgtataacct gtattgtggg cagattagga
     4681 taaccaccaa ggatagccgg tatggcctta tcagttctgg agcctgttgc ataaaagttt
     4741 gagaaataaa ttccttctgg tatgaaactg ttaaatctgg gggcaacaac cgaatctccc
     4801 tcccttgctg aaattatttc acttccaaaa ctttcgacaa taataagaag aatattgggt
     4861 ctctgagtgt taagtaacat cgtcgtttta ccttcgtcaa gcattaaatt tttgaaattg
     4921 ccgagtgctt cttcttttga agtgaattcg tatggatttt ttaatggttt caggtaagca
     4981 gtcgagtgtc cgaaatgcca tacttcattc agcgcagcat ggttcaggaa aaggtgctca
     5041 ctgaaataca catgaccggc attcagggga gcaaccccga aaccacccct caccggaaca
     5101 atcagcaatc cggtaagtat aattacaagc gaggtcttta acagtacaaa tctcttttca
     5161 gggacaaacg aatagaatct ttttataaac cattttagac ctttcagctg caggtaaatc
     5221 aataacgcag aaacactcac tccacctatt aattgaagcg ttgatgctga tgcaaaagca
     5281 tcttttggat ccctgagata agcaactaca gaaaagtcaa ctctgtatcc ccagtatgaa
     5341 tatactactg catccccaag aacaagtaag gtgaagaata taatcagaaa tgccgaatat
     5401 attttcataa atttctggta ccattttccg gcaaacacaa accagaatgc gcccagaaat
     5461 aatggcagaa tcaatatgta tgcggtaacg gaaagatcca ttttgaaacc atgcagaaaa
     5521 gtttgaataa tatctgcaaa tgagtaccgc accatctctt tgaattgcat tagcaggaaa
     5581 atcactctgg caaaaaagaa aaatgccagc cagaaaaagc tgtagaatat tgtagcagtc
     5641 agaaattttt tcaatttcta taaaattttg aagttcacaa aagtaatatt ttgaacatga
     5701 tttttgtatg atttttattt tattgaggaa cttatgatat atttaaattg aaatttgtat
     5761 caggagtcat gacagttgca gaatatattg ccgaaagact tttttctgag ggtgtaaggt
     5821 atgtttttgg cgtgcccggg ggagcttcaa taccattaat cgaagcaatt cgtcaggcag
     5881 gtatagcatt tgtacttacc gccgaagaaa gaagtgcagg ttttatggcc gatgtaactg
     5941 ccagaataac aggtattcca ggcgtctgtc attcgacatt cggacctggc gctaccaata
     6001 tgagtaccgg agttggctgt gctctgctcg acaggtctcc tcttctggcg ctgacaagcg
     6061 aaatgcccga tgattggatt gatcggacca cacagatgaa cattaaccac caggctctct
     6121 ttaaaccggt tacaaaagca acattcagac tttcacctag aaatgttcag agtgttttgc
     6181 ccagggctct aagtatatgt aatgaagaat atcccgggcc cgttcacctt ggaataccct
     6241 ccgacattgc cgggaatcag gtttcatcag atgtgaaaag ccaggaagta aggcgtcagc
     6301 aaatacctga aaataatcat gatattacag aaataaagaa tttactgaag agtagtaaaa
     6361 acccggtaat tgccattggt cttaccgcag cacgcctttg taatagcagg caggttaata
     6421 ctctgctcac aggttttcag gtgccggtgt tacttactcc aatggctaaa gggctcattg
     6481 atgaagaaca tccatgctat gccggagtac tttcgcatgc acttagtgat tatcttgaag
     6541 atatcatttc tcaggcagat cttgttattg gactgggata tgacccggtg gaatacaatt
     6601 atgaatcgtg gatgccggat gtgccattga ttcattttga tacagttttg caagacttgc
     6661 cccgaagcag ggatgtttac agttttgtgg gagacccgac agattggttt gcaatacttt
     6721 cagaattgaa actaacttcc gggattatca gctcaggctt gctggctgaa gtcagggagg
     6781 agatggattc tgttttccgg ggatttactt ccggtttcgg acctgtgaca gttattaaag
     6841 tgcttggaga aatgctcccg cgtgatacaa ttgctacagt agatgttggc tcacatcttc
     6901 atctggcagg acaatactgg aagacgcatg gttttcagaa tctgataatg actaacggct
     6961 ggtcgtcgat gggcttcggc ctgcccgctg ccatagcagc aaaacttaat aaaccttctt
     7021 ccactgtggt atgcctgact ggtgatggtg gattcctcat gtcggcaggc gaagtactta
     7081 ctgcccgccg ctgcctggca ccaataatta tcgtggtatt ctccgatggc gaacttaacc
     7141 ttattaagct aaaacagaca tggaaaggac ttcagcctta cggcatccat ataagctcag
     7201 gcgacctgtt tggttctgaa tactttctcg gtgcaaaagt ctttaatgcc acatctgaaa
     7261 ccactctgag ggatgcagta atgaatgcat tgattcttga tgagacagtg attatcaatg
     7321 caataataga ccctgaagat tataagtggc ttgttgttaa acagaaataa atcaccttcc
     7381 gcaagaggat tctctcatcc ggtatttact tgttatgctg tcttttcagt ttatcggaat
     7441 caggcttaag gggagtctat tctttcccgg tttttttccc tgaagatgac gcctttttct
     7501 cttttttctc tccttcttca gattttttct ttgtatcttt agctgcgggt tctttcttct
     7561 cttttgcggt tttcttctct gcggtttcct tctttacacc tgattctttc tttgcagcag
     7621 tctctttact ggctaccggt tccttttttg cagcggcctc tttctttgct tttggtgcct
     7681 ttttctcaac ttccttctca acagttacct gttcagtaac ctctgccttt tcttcaatct
     7741 tttcaatcac ctcctgtgta acagcttcca ctgcaacagc tggttcaacc acttcaactg
     7801 cctctttctt cttttctctt acaggcttct ttactgcttc ttcaactttt acaatttcag
     7861 gctgtagtgc cggttttttt gattttttct ttctgggacg ccttactcca tatgtcccca
     7921 ggaatatttt tcctcttctg ctttttctgt ctccttttcc catataattt atttttgatt
     7981 ccttataaaa aaatcatttg aaatgagagg caaagttata aaaattcttt actccgactg
     8041 aattttgata tttcattgag ggaaaaattt tagaaaatta tagtcttttc agcatttatt
     8101 gttgtaaata tttttttatt cggaaaataa acctcacctt tgccgtgttt taacatttaa
     8161 tttttttgta attatgaaca tttacgttgg aagcctgaat tttaaaatgc aggaatcaga
     8221 attaaaagaa atcttcgagg agtatggtga agtaacttca gccagaatta tcacagataa
     8281 atactccgga agaagcaaag gtttcggttt tgtcgaaatg cccgatgaag ctgatgccag
     8341 aagagccatc aaggaactta atggagcaga agttggcgga agaaccataa tcgtcaacga
     8401 atccatcgaa agaccagaac gcagaaattt cagaaacggt aacaatgacc gcagaagaag
     8461 agacagctac aggtaggaat taaaaagatt aacaagatga aaggaaacgt taaatggtac
     8521 gatagtgtta aaggttatgg atttatccag accgaagaca acaaggacat ctttgtacat
     8581 cgtaccggaa tagaaggttc ttaccggaaa ctggaaacag gagaagctgt tgaatttgaa
     8641 atagaggaga gagaaaaagg gcctgtcgct ataaatgtca ggaagtcagt ataatctgtt
     8701 tctgatatat taatttttga ggggcataaa aaatttttat gcccctcaaa attttacacc
     8761 ccttcaaaat tttgtgccac tatttttcat atgtgacact aataaaaaaa atctggcaaa
     8821 aatcaatgat gtaattatag caggattcat atttttctaa atttgtatgt atggatccct
     8881 ggatgttaaa aatatttaca cgatgtaatt cacccagatt gaaatacgta gctgacctta
     8941 tttttttcag catcctggga gtaccttatg aaatagttac tgaccgcaga cggattggaa
     9001 gaaaccctgt tatcaactat tccggagaaa aaattgatga cgcattcaga atttcaccat
     9061 cgggtctgct gggtgaaaca ggtataaaac cctttgatat tgaggtttct gaatggaaaa
     9121 acctgccaat ttttttccgt acagagacag atgcagactt tccttttgac attttttctg
     9181 caatatttta catggtaaca aggtatgaag aatatcttcc attcaaacca gatactcacg
     9241 ggaggttccc tgcctcaatg agccttgcat tcagaaacgg ctttctgaag aaacccgttg
     9301 tgaatctgtg gattaaagag cttgctgctg aattggttat tcatttcccg gaacttgttt
     9361 tcaaaaaaaa tagcttcaaa tcatttgtta cattcgatgt tgatgaacca ttcaaatacc
     9421 ttggaaaaga ccttttcaga aacttcggga gaatgctgct cgatttaggt aaaaaaaatt
     9481 tcagttccgc tgaacgctac aggacaatca aagggaaatt gaaagacccc tggaatattt
     9541 tcgattatct tatcgaaaaa acagaaaatt ccggctctga catattgatt tttgttccga
     9601 caggcgaccg gtccgaattc gacagatggc ccacatggaa caacgaagac tatgctgagc
     9661 ttctggctgc aatcagaagc aagaccagaa tcggactcca cccttcttac ttttcaagcg
     9721 ataacataaa aaagctaaaa accgaaaaag ccaggttaga gtcactgatg gcatctgaaa
     9781 tattctctgc aagatatcat tacataagac tgaaattacc ggtatcatat tcaaacctcg
     9841 aagaagctgg aatacgtgag gactattcaa tgggttttgc cgatgaaccc ggtttcaggg
     9901 caggtattgc aacacctttc tatttttatg atctaaacaa ggaaaataaa acaaacctga
     9961 tggtttatcc gtttcaggtt atggatgcca cactggtgca atacagaaaa atgaacccgg
    10021 gcgatgcaca atgccttatt gaggacctta ttgacgaaac cagattggcg ggaggaattt
    10081 ttcagtcggt gtggcataat aatatacttg ctgaaggcga cgctgcaatg gaatggagag
    10141 ctgtttttga aaataccctt aaatatcaga atagatgatt atatatctta aaaacaagga
    10201 aatagacaga aatctctggg atacatgcct gaaaaactca aggtgtctca aaccatatgc
    10261 ttactcatgg tacctcgata taatggcacc gggatgggaa gcacttatcg acgatgagta
    10321 cgactctgtt tttccagtcc cgggatttga gaaattcgga attaaataca taaccacacc
    10381 tatctttctg caacaactcg gagcattctc acctgataaa ccggccgaaa aagttattca
    10441 tgaattcata ttttatatgc ctgaatttta caggtttgta gacctatgcg taaactacag
    10501 ggttgataat aaagattata aaattactga aagaaccaac tatgttctgc atctgaacag
    10561 agatttcaat gaaatctgga attcgtattc atcagattgc agaagaaata tcaccctcgc
    10621 ttcacagaac agcatcaact acacaacaga tataaaaccg aaagaaataa taaatctttt
    10681 tatcgaaaat accggaaaaa atatccccgg gataaaacag agggattatg aacgactcga
    10741 aaccctgatg aaccattgtc tttttaacag gaaaggacgt attatcggtg tccgttcagg
    10801 tgatggcagg ttgattttcg gtattttcct tgtcgaaatc ccgggttcaa aaacaattct
    10861 ctttacagca aacagttctg aaagcagaga aaaaagaata aactatcatg ctgttaatga
    10921 gttgattaaa gaaaatgcat ccactggcat cgtgctcgat tttgccggct cgtcaatacc
    10981 ctcagttgca tcttttatgc aatctttcgg cagctcaaaa gagacatact acaggttata
    11041 cagaaattcc cttccctggc cactgagaat gctgaaataa cttcttaata tttttagaag
    11101 atttttgtaa cgaaagtttt tccttcagcg aacagtaagg ttataatgat tccttaatta
    11161 taatattgtt gaatttcaga tcagttcaat attttcttcc tggtcaactt ccagaagctt
    11221 tccatcttca cacttcaggg ttcttgccgg gtatctttta agcagattct gattatgtgt
    11281 ggcaactatg atagccctgc ccgtctgatt gatttccatc aaaagcctca gaattccgtc
    11341 cgaagtctca ttgtcaaggt ttccggtcgg ttcatcggca agaatgatat caggatcgtt
    11401 gagaagggcc ctccctataa caacacgttg ctgttctcca cctgaaagct gatgggtcat
    11461 tttgtagcct ttaagtccca gccccacctt ttcaagaact tcagcaatcc ttgtttcaat
    11521 atcattctta ttcttccatc ctgtagccct cagaacaaat tcaagattat cgtgcacatt
    11581 cctgtcggtt aacagctgaa aatcctgaaa aacaatccca agcctccttc taagataagg
    11641 tatatctttt ctcctaagtt ttttcagatt atatccagct acgaaggctt ccccttcctt
    11701 aagcggaagc tgtgcattta tagtcttaat cagactcgtt tttccgcttc ctaccctgcc
    11761 aatgagatac ataaactcat tccttttcac ggaaaagtta acgtcagtaa ggaccagatg
    11821 atcctgctgc cagatgttac agttcctgaa ctcaattatc gtatcaaggg aatgagcttc
    11881 ctccatatag cggcaatttt gtccacaaaa taaagaaaga attgataact aacatagcga
    11941 tgttaataaa ttcggtaata aagccattca ggcaccgttt aatagttgta attttacgcc
    12001 acatagaaag ccgcacataa gctaattatg tgacatttaa acagtttgtt ctgttagcgg
    12061 gaaaatttta aataatggtg aaaaagtgat tataattcaa gcttttaatt aattatgaac
    12121 aagaggctgc ttttgatatt gtttttggtg ctgccggcag ttcagcttgc tgctcagatc
    12181 tcttacatga ataaggaagt cagctcggaa tttcagaagg ggcttgaact ctacaacaag
    12241 gagaaatata cagctgccct gaaatttttt gattcctggc ttaaaaactc acagggcgat
    12301 aatgcaaacg aaaagttcga tgctgaattt tacagcgcca tgtcagcatt gaagcttttc
    12361 aaccccgacg gtgagtaccg gatgaacaga tttatcgcct catggcccgg ctcttcacgg
    12421 attaatgacg cttaccttgc tctgggtgac tacttctacc agaacaaaaa ctaccgtaag
    12481 gcagcaactt attatcagcg cgtgaaccgt atactgcttc cttcagaaaa acttcacgaa
    12541 tattgtttta aatatggata ttctcagttc atgaatggaa acaaagagat agccctttta
    12601 atgttcagcg agataaaaga tattgataca tattacactt cccctgcact atattatttc
    12661 tctcacattg cctatgagga gaagaaatat gaaactgcaa tggaagggtt catgagactg
    12721 agaaatgatg aaaccttcgg aagcgtggtg cctttttata ttgttcaggt attgtatctg
    12781 agaaaagatt atgatggaat acttgaaatg gctcctgaac ttctgaagtc ggcaggtaag
    12841 atgagagccg tagaacttta caggtttata ggtgatgcat actacaataa aggcaactac
    12901 aaagaagccg caggctacct cgaaaaatat gctgaagggg caaaagccag cgaccggccc
    12961 gaccggttcc agcttggcta ctgctactat atgacaggcg actatgataa agccattaaa
    13021 atattcctcg acctcacagc ccgctcagat attctgacac agaatgtttg gtacatcctt
    13081 ggcgactgtt acctgaagaa aggcgataaa caacgcgcac agtttgcttt cgggcaggca
    13141 tcacagctta atttcgatcc gaagataaaa gaagatgcac tgtttaacta tgcaaagctg
    13201 ctttacgaaa cttcttattc accttttggc gaggcaataa gagcatttca ggaatatatc
    13261 aacctctatc caggctccga ccgcatcgag gaggtctata atttcctcgt tgcaacatat
    13321 atgcagatta aaaactacag ggaagccctt gcatcactcg ataaaatcac acgcaaagac
    13381 gcacgtctcg aggcagctta tcagcgcgtt gcattctaca gaggactcga actgtttaaa
    13441 aatatggaac tcacaacagc tattaacatg ttcgacaaat cgctcgatta tggaaaatat
    13501 aacaatcaac tgcgggcaag ggcaatatac tggaggggag aagcatggta ccggcttgga
    13561 gaatacgaca acgcaatagc tgactaccag acttttatgg gattacctgg ctcggcacaa
    13621 ctcgatgaat accggcttgt tagatataac ctcggttatg cacttttcaa taaaaaagac
    13681 tataccaacg ccctggctca tttccggaac ttcgaatcaa atgccacagc tgttagagcc
    13741 gatatcctgg ttgatgcacg aaacagaata gcagattgtt atttcattaa cacaaattat
    13801 gccgatgcgg ttgaattcta taacaaggtg attgagttcg gcaaaataga tgcagattac
    13861 gccctctacc agaaaggatt tgcactcgga ctcatgaata accagaaggg taaagcagag
    13921 accctgacaa ttcttattaa caattaccca tcttcaaaat atattccggg tgcattgttc
    13981 gagaggggta gggcatgggt aagcctgaag gacaaagcca ggggagaagc cgatttcaac
    14041 caggttatat caaccttccc gggaagccca ttcgcaccga gggctattat tcaactcgga
    14101 ctcctctatt ttgatacagg agaaaacgaa aaagctatag cacagttcaa aagagtgata
    14161 gaaaactaca gctcaacacc cgaagcacgc tatgcactta ccggacttaa gaatgcctat
    14221 gtagaaataa acgacgttga ctcatatttc gcttatgtaa aatctcttaa aggatttggc
    14281 gatgttgccc agacagaaaa ggattctttg ctttatgtat cgggcgaaag attgtatatg
    14341 tcgggcaatt gcgaccgtgc atcagaagta ttcagaaact acctcaacga atttacatac
    14401 ggcagcttcc ggattaatgc tctcttctac cttgcagaat gcgaaatgct gaaaggaagg
    14461 aaagatgagg cacttgataa ctaccagaaa gttattgagt cgcccggaag tgaattttat
    14521 gagccagccc tgcggtcggc tgctgcacta ttgttcgaaa aagaagatta cctgaaagca
    14581 ttcggattat acgaacaact tgaacgtatt acagacaatt ctgaaagtca ggtgattgca
    14641 ttgagagggc agctccgtgc agcatatgaa gccggcgatg cggagaaaac catcagaaca
    14701 gctgaaaaga tcaacaaagc acaaaatgtt ccggaagagc ttctcaggga agcaaacttt
    14761 atgagcgcta aagcacacta cagccttaat cattatgacg aggcactgag ggacttccgg
    14821 aaaaccgctg ttgagataac cagtgcacag ggtgcagaat cgaaatacag ggtagccgag
    14881 atacttgttc tgaagggaaa catcgcagaa gccgaaaaag ttattaatga attcattaat
    14941 tccaccaccc cgcatcagta ctggatggca aagatgtttc tcctgctggc cgatgtaagc
    15001 cttaaaaagg gagatacaat aatggcaaaa gccaccctgc agggacttaa agaaagctat
    15061 tcagtaccgg atgacggcat actcgacgag gtaaaagcta aacttgattc cctgaatgcc
    15121 ggacaaatac aatagccaaa acactaaagg acgatgagga atatgaaaat gaaaataaac
    15181 ataatgaagc cgctgatgat tattactgct tttctggctt tttctgctct attccctgcc
    15241 agaggacagg aaacagtaat aaaaagggaa ataacactct acaaccctta taaaccttca
    15301 ttgcccgatg ttgtaaaaaa gagcttcctc cccgacatga cagatacatc agctgtaaaa
    15361 ccggtgatca aatatgaaat ccggacaaca ccctacacgc ctccatatac ggttagccca
    15421 cttaaggctg caactatggt acccgaccca cttacaaaac tctataacgg ttatataaat
    15481 atgggcctcg gcaattacct gacccccctt gctgaaataa gcataacaaa cctgagatcc
    15541 aaacagagcg gcgttggtat ttatgctgga catttctcca caaacggtaa ggtgaagctt
    15601 gaaaacggca aaaaagagtt tgctggttac atggataata atgtgtcact ttacggaaga
    15661 aaattcctgg atgaaagcgt acttaaggga agtatcgact tttcccgtaa aaccaggtat
    15721 gcatacggat ataatccagg gataacagat tatgaccctg aaaagaagga tttcagactg
    15781 aattatactt cggcaggaat aaatgctgga atatcttctg tgagaccaga ttcctcaaaa
    15841 ctggtatatg aagctaatct tgattataat tttttcatat cagaaaaaaa catgtacctg
    15901 cacacagtga aattgaccgg ccaggcagga aaggaggtag aaggttttta tgctggttca
    15961 ggagcgggct ttgagtttta taagccttcg ggttatattt cttcatcttc agcatatatt
    16021 gcagaaataa caccttttat taccaggaag agcgccgact ggaatgctaa gctgggtgca
    16081 ggatttgcgc tggacaaatt tattgatgat gatgacccca agttacatat ttaccccgac
    16141 ctcagattcg gatttaatat aatgccttca tacctggctt tttatgcgga acttaaagga
    16201 aaacttgaaa gaaacacacc tgaaaatgtt atcatgctta atcctttcat gctttcagat
    16261 tatctatata aaataaggaa tacatgctat cccttaattg taagtgccgg actggaaggg
    16321 gagtcgggca ttgaaggcag atacaggtta aatgtgtcat atacaattgc agatgatttt
    16381 cttttgttta cgaattatgc aggaacaagg aatgacacta ttaatatatt gatgggtaat
    16441 tactttaaac cactatttga tgaggctgaa atactgaaat ttcacggaga gacagccggc
    16501 cgtatcaaca ggcatttcac cttttcagcc gaaggaaatt attataaata cacacttaca
    16561 agcaataaat atgcatgggg tatgcccgat tgggatctta aatttaatct gaaatataat
    16621 ctcagggaca agataattgc ttcaattggg gtaaacacaa ccgggccaag aaaactgctg
    16681 acaactactg ataattttga caatctgttt aatttactgg gaagctcgga gaaagaatat
    16741 agcatgcctg cccatctgaa ctttaatttc agtgccgaat acagatatac aaaaattcta
    16801 tcgttctggc taaaattcaa caatctgtct ttcacaaaat actacgaatg ggcatggtat
    16861 ccatcactcc ggtttatctg catggcggga tttacttaca gcctgtaaat tgtatttgta
    16921 tttagcaggt tttcagtctt catatatatt tgttaattaa caaattatgc catgttaatt
    16981 acttggtaaa atagttaata ttttttaact cttccctgat aaacataagg atattatttg
    17041 tatatttgca acgataatta atttacatta tatcaaatat ttatcaaggg tttgagaagt
    17101 tggatccggg ggcagcgcag atatgtaaga atctgattct ccttaaaaaa tgcctgttca
    17161 acttcaggac agaccgtaaa agggtctgct gataggaatt tataattttt aaactgaaaa
    17221 acagtaagtt aaacaaatat aaaaatgagt gataaactga aaaaggaaaa taacgggaac
    17281 ggttattccg ccgatagcat tcaggtactc gaaggccttg aggcagtaag gaaaaggcct
    17341 gctatgtaca tcggcgatac aggggtgaaa ggtcttcacc atcttgttta tgaggttatt
    17401 gataattcta tagatgaggc tcttgcaggt tattgcgata gaatcgatgt tgtaattcat
    17461 gaagatggct cggtatcggt gaccgataac ggccgtggta tacctacaga tatccatgaa
    17521 aaagaacaga agtcagctct tgaggtggta atgactatac tccacgcagg tggtaaattc
    17581 gataaggata cctacaaagt gtcaggaggc cttcacggag tgggcgtctc ggttgttaat
    17641 gcactctcag aatggctcga agctatcatc catcgcgatg gaaaagtttt ctaccagaaa
    17701 tacgagagag gtaaacctgt taccgatgta aaaattattg ataaaacaga taaaacagga
    17761 acaattgtaa gatttaaacc tgacattcag atttttcaga caattgattt caattttgag
    17821 atactttctg caaggcttcg tgagctctct ttccttaata aaggggtaat gctgacgata
    17881 aaggaccttc gcgaacctgt tgaaaacggc aacggaaaca aaataagata cgaggaattt
    17941 attagccaga atggactgat tgaatttgtt aagtatcttg attccaacag agaacctctt
    18001 attgagaagc caatatacat atcaaccgat aaatatggtt tccccgttga ggttgcaatg
    18061 caatacaaca cctcatttac tgaaaatgtc tattcatacg taaataatat caatacaatc
    18121 gagggtggca ctcaccttgc tggtttcagg aaaggactta cacgcaccct taaaaaatat
    18181 gctgaagagt cgggtgctac tgcaaaactg aagttcgata taaacggtga cgatttcagg
    18241 gaaggcctta ccgcggttat ctcggttaag gttcctgaac cccagtttga agggcagacc
    18301 aagatgaaac ttggtaacac cgaggttatg ggtgctgtcg accaggctgt tggtaatgct
    18361 cttgccaact acctcgagga aaatccgcgt gacgcccggc agatagttca gaaagtaatc
    18421 cttgccgcaa cagcccgcca tgccgcaaga aaggcacggg aactcgtgca gagaaaaaac
    18481 cttctgtcag gttcaggact ccccggaaaa cttgccgact gcgccgaccg tgacccgtca
    18541 aaatgcgaac tctttatcgt tgagggagac tcagcaggag gaacagcaaa acagggacgc
    18601 gaccgccggt tccaggctat cctcccattg agaggtaaaa tcctcaacgt cgagaaagct
    18661 atgcagcaca agatattcga aagcgaggag ataaggaata tttttaccgc cctcggactt
    18721 tcaataggta ccgaagagga tagtaaagcc ctcaacacat caggcctgcg ctaccacaaa
    18781 ataatcatta tgaccgatgc cgacgtcgac ggctcgcaca tcacaaccct tatactcaca
    18841 ttcttcttca ggtatatgca ggaccttatc aagaatggaa atatattcat tgcctcgcct
    18901 cccttatatc ttgttaaaaa aggaaagatc gaaaaatatt gctggacaga agaagaacgc
    18961 gaagctgctg ttaaggaact cggccagggc aaagactcat cagtaagcgt ccagagatac
    19021 aaaggtctgg gtgaaatgaa cgccgaacaa ctctggcaga caaccatgaa ccccgagact
    19081 cgcatcctca agcaggttac aatagaaagc gctgccgaag ccgaccatat cttctccatg
    19141 ctgatgggag aagaagtacc gccacggagg gagttcattg aaacacatgc aaaatacgca
    19201 agaatagatg cataaagggg aaagctatac accaaaaagc ttcctcctta tctctattgc
    19261 gctgtcggtg cagacttcac tgca
//
LOCUS       DAOSNB010000012        18962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_2918_length_18962_cov_4.311948, whole genome shotgun sequence.
ACCESSION   DAOSNB010000012 DAOSNB010000000
VERSION     DAOSNB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2918_length_18962_cov_4.311948"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1399)
                     /locus_tag="PLG42_01260"
     CDS             complement(80..1399)
                     /locus_tag="PLG42_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29868.1"
                     /translation="MADWHSNIDLVFRNGLKNHEVLPPEDVWENVSRSLPERRRKIYP
                     ALMRAAAFATLVLSTGTAVYFLHNKLTDTLANAPALTLNQDAMPEGQFLASEHNSGIA
                     AQLQKPAIRMPVVTAMEETSQKEENNIDISGIPSSMISENNTFKTSNNELNLANLRYL
                     DVPGKVKSKTFDITYIPEERGSSEGEKWKVGASVLPSYHSRFSFGNDDAAADYLKSEK
                     MAFSYSGGLSFTFEASRRLSFVSGIFYSSIGQRIDGITSFTGFSKYNESKSASDFSIL
                     TSSGTISSTNKDIYFVDNSNVARVQTRYSADIFDPMKANLSHLSNSLIQSMSYLEIPL
                     MMKYKLIDRKVDINMLGGLSYGILIGNTSYINSGGLKYTIGHTEGLSPVTFSSSVGMG
                     MEYKLTTNISFNLEPVLRYYITPLGGITGSMVHPYSFGILSGIFYNF"
     gene            complement(1392..1952)
                     /locus_tag="PLG42_01265"
     CDS             complement(1392..1952)
                     /locus_tag="PLG42_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU29869.1"
                     /translation="MDDIKEIIKGCLEGNRRDQELLYRRHCSKLYAVCLQYSSDDEEA
                     RDILQEGFIKIFQNLHSYKHEGSFEGWLRKIVVNTALEKYRAKHNLYRVDDLDTIDEP
                     QAEPDTEDYTGIDAADLLYMIRELPPKYRMVFNLYAIEGYSHKEISKMLNISEGTSKS
                     NLSRARTILQKKVKLYTGIRKTIING"
     gene            complement(2089..3480)
                     /gene="lpdA"
                     /locus_tag="PLG42_01270"
     CDS             complement(2089..3480)
                     /gene="lpdA"
                     /locus_tag="PLG42_01270"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOU29870.1"
                     /translation="MDYDLLIIGSGPGGYVAAIRASQLKMKVAVVERAELGGVCLNWG
                     CIPTKALLKSAQVYQYFMHASQYGISVQGEVKPDFKAIIARSRAVAEGMSKGIQFLFK
                     KNNIVHIKGTGRLKGAGKVEVTDDSGNRKVYTAANIILATGARPKALPGMEIDGKKII
                     GYREAMTLPEQPASMVIVGSGAIGCEFACFYQALGTEVTLVEIMSQIMPLEDEEVAKQ
                     LERSFKKMKMKVLTSSTVSSVDTTGNKCHVTVKTPSGNQVIEADVVLSAAGITPNTEN
                     LGLEEAGVRTHDGKVMVDEFYRTSVNGIYAIGDIVKGPALAHVASAEGIICVEKMAGL
                     DVEPLNYDNIPSCTYTMPEVASCGLTEKAAARAGYEIKVGKFPFTASGKASAAGDREG
                     FVKLIFNAKDGELLGAHMIGQNVTEMIGEIIVARKLETTAYEIIKSVHPHPTLSEAIM
                     EAAAAAYGEAINL"
     gene            complement(3634..4449)
                     /locus_tag="PLG42_01275"
     CDS             complement(3634..4449)
                     /locus_tag="PLG42_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOU29871.1"
                     /translation="MPVLQYDFILKGILGCLFASITAGIAGTYIVSRRMVFLSGGITH
                     ASFGGIGIGYFAGVNPVAGAAVFGILSAIGIEYLSVKQKIREDSAIGIIWALGMAVGI
                     IFIYLTPGYTPNLISYLFGSILTVTNADLIALGILSVILLLYFSVFYRTILYISFDEN
                     YARTYSSYVDFFKYLTTSLMALTIVLNIRMAGVVLVLSLLTIPPNIAMLFTRKYSSIM
                     KLSVLFGFAGTSGGYLFSWFTGIPAGAATIFVLVVMWLILKLITMVNLKREME"
     gene            complement(4458..5204)
                     /locus_tag="PLG42_01280"
     CDS             complement(4458..5204)
                     /locus_tag="PLG42_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU29872.1"
                     /translation="MKKLVELIDVTAGYNSKVVLEDVNLTVYEEDFIGIIGPNGGGKT
                     TLLKVILGMVKPMKGTVIYNHEMLKRTDIGYLPQLSSVDISFPLTVTDVILSGLMMHK
                     GVYVKMNREDRKKADGIIDEMGLKGFEKATLAELSGGQIQRVFLGRALIGNPRLLLLD
                     EPDNFVDTTFENEFYIKLHELNSRMAILMASHDVGTISYHVKSYACVNRHLHYHPSPE
                     LTEEQLSAYNCPIQLVTHGPVPHTVLKKHD"
     gene            complement(5201..6079)
                     /locus_tag="PLG42_01285"
     CDS             complement(5201..6079)
                     /locus_tag="PLG42_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOU29873.1"
                     /translation="MRKEFLFLILLSLLSCKPNTFSTDKGLITVSIAPFGFFVEKIGG
                     GDFIVNVMVPEGADPHVYEPAPAQVAALRKSSAYISDGYLDFELAWLDKFYSVNPSMK
                     KLKLADSVELINEETEVNEGIMLYADPHFWVAPLSGLKIAESVKSLLCELKPDSCEKY
                     TLNFHAVEDSIFKIHKRASELFAPFAGHSFVVFHHTLAYIAKDYNLIQVSIEKEGKEP
                     SPGWLREVIDMAKKNRTGVILIQKEYDTRNARVVASESGAGIREIRPLSADWYGAVND
                     IIDAIYESFTASDSKK"
     gene            6198..6692
                     /locus_tag="PLG42_01290"
     CDS             6198..6692
                     /locus_tag="PLG42_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29874.1"
                     /translation="MKNKGEILAEILREYDFCYFFLHNTRDINTVNKILNEGFRYESQ
                     LPHSTDRVNPHEPIEIVYFLFQRKEYGPYTVVIAIPRETYERYTRLSNILDTTIEEVM
                     STEKPYYSENDELVYTISPKHILGYFNNNTSEFVKNEHWDPLFDISQLKTFKRRPPAD
                     AKPK"
     gene            7378..9048
                     /locus_tag="PLG42_01295"
     CDS             7378..9048
                     /locus_tag="PLG42_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU29875.1"
                     /translation="MVKRLLKFTFLLVVISSIFISESLAQFANHRALSQKATKLATDY
                     PALCSVKSLVKTPGGRDVWVLAIGTGERDSKPAIAIVGGIDGSYIFTRELAIGFAENI
                     LKESATPEIKNLLDRITFYVFPDVNPDASEQYFSTLRYERLINDRPTDDDRDFQTGED
                     PFEDLNGDGLITLLRVSDPAGKYIESTDDKRVMVEADLSKGQKGTYLLFSEGIDNDKD
                     GLFNEDGAGGVDFNKNFTYNYEEYGANAGLHAVSEPESKAVADFLYDHFNIYAVICFG
                     PQDNLSQQTPRGNERPAPSGQQTQAIQQQQGRQAGERRITSVLRSDETVIRLVSEKFQ
                     EITGLRGAPPSKTSPGNFADWVYYHYGRYCFTTPGWWFPSERGKNTEVSFLKFAEDNK
                     LENVFVPWTEIKHPDFPDKKVEVGGIKPFAMLTPPADKVQEMITKNYKFIVEVAGMHP
                     ELEFIDKKVENVGSDIYRITLKVHNKGLFATCSEAGANNQWTRIMRISLEPAKGQTIL
                     SGQKVQRMQRLEGNSSSEFSWLISGKGRVAISAGALNTGTISTSFDLK"
     gene            9070..10866
                     /locus_tag="PLG42_01300"
     CDS             9070..10866
                     /locus_tag="PLG42_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU29876.1"
                     /translation="MKSIKKISLIILLMVLTSAASNAQKEELFFRAAGSPPDPKVQAS
                     WNKYYTNAGIYDLCSKLAKAYPNLVSLESIGKSYQGRDIWLLTISDKKAGNPDKKPGY
                     WIDGNIHSIELQGTEMALYTAWYLCEMYNTNTFIKELLAERTFYIAPTINPDAREYFT
                     SVGVPPRSGLAPRDNDRDGLFDEDGFDDLNGDGEISQMRRKNPEGNYIVDPKDNRRMI
                     RVEPGQKGEYELLGQEGIDNDGDGLVNEDGPGGYDGNRDWGFNWEPNYIQSGADKYPF
                     TFPENQAVRDFLLKHRNIAGAQSFHNSGGMILRGPSVQSGYAEAYSRADDAVIEALGK
                     TGEKILPGYRLLTVWKDLYTVYGGEVDWMHGAMGCFVFSNELWTPYLMFYDTTNTDQY
                     EFDRLLLFEDAFIPWQKINHPVYGEVEIGGFSKNYGRLHPGFLLESDAHRNAAFCIYN
                     AYHLPKLEVTKVTVKSLGGGLKEVSATIENKRMVPTHSASNLRYKIDPPDYVYIEGAQ
                     SVLAGMIVENEDLNICREQKKNPARIEIENIPGYLGGGAQTGGGGFAGGRSPGAAGNS
                     NIIKVKWIVKGGDRYTVRVESVKGGTASAQSN"
     gene            complement(10946..11626)
                     /locus_tag="PLG42_01305"
     CDS             complement(10946..11626)
                     /locus_tag="PLG42_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29877.1"
                     /translation="MKLKVLYTGVLSALILGMVVAGAAGQEKTQEQKEKEARLQQAIE
                     QQKKAMQEQAKIMEQQAGEIQKAVVQEQGRPEGVRPDDPSRRSRTNVFIGREGADAGA
                     FYIGPGGDFGGLSYFRGSGTSFEFSKGLNEATITRQLPFEVDSDAKNVVMSISGDCKT
                     GEIKVKILSPDGKTYAEVLIDTSGNLNWRKSFTVSGEENKDKIGEWTFKVETTKATGY
                     FRISVQTF"
     gene            11778..13166
                     /locus_tag="PLG42_01310"
     CDS             11778..13166
                     /locus_tag="PLG42_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU29878.1"
                     /translation="MRLKNSILVAGIISIVILIIVQVLVIKDIWFDKDVIFDLKYKLL
                     SQEAMEQLNRMSQNDPFENALNFLNDYSRQVMTTEIQNVKNDEDLTRLKEEIMRDVNK
                     ILTELENLSPYLKGYFNKQGFESDFSIKIEVNFLRLIDFEKRFDVYANPQMFAGPPVS
                     SANVTKDTRSKILVNRFFREDNYYRIAFDYYIDISGKQRMVLRDMALYLALSIFSIIV
                     VMIIFYITYHNLMEEKRLSNLKTDFINNMTHEIKTPLSTITVAGKTLEMEQIRTNENK
                     VLETARLIHKQSVHLNQLINLILEISMWERTQFQLDKKEVDLRELMHDIVESFRTAQN
                     NNVQIIENYGFDTLKTKLDVVYFTTMLNNLLSNAVKYSDKEPVIKVEGQVNKENIVIS
                     VTDNGIGISKQDQKHIFDKFYRVSHGNIHKVKGLGLGLYYVKRIAEAHGGTVTVSSKP
                     GEGSCFTVTIPK"
     gene            13182..13901
                     /locus_tag="PLG42_01315"
     CDS             13182..13901
                     /locus_tag="PLG42_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU29879.1"
                     /translation="MKVLIAEDDRDFGNVLAQYVTISGFEVTLARDGKEAWELFNKDR
                     PDICVLDVMMPEMDGFTLAEKIKEAAPEMPFIFLTAKSLKEDIVRGLKLGADDYITKP
                     FDPEVLILRINNILKRAYSSVNDEYKISDTILKFNTLELISGKKKEKLTLKEAQLLRY
                     FIMNKNKILTREDILTEIWGEDDYFLGRSMDVFISRLRKYLSEDKGIDIRTVRGTGFI
                     LEELGSGMQEKDEEEKTEPEM"
     gene            complement(13911..15008)
                     /gene="meaB"
                     /locus_tag="PLG42_01320"
     CDS             complement(13911..15008)
                     /gene="meaB"
                     /locus_tag="PLG42_01320"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOU29880.1"
                     /translation="MVQMPDKQKKGSALSIRNGIEQPPLVNPEILKKNIAGKRKILSA
                     GEYISGILSGNRAVLAKAITLIESSNPNHYSLAQEVVEGCLQYSGNSVRIGITGIPGA
                     GKSTFIESFGTMLTGMGKKVAVLAIDPSSKKTKGSILGDKTRMERLSTDPNAFIRPSP
                     SAGTLGGVARKTRESIILCEAAGFDTILIETVGVGQSETAVASMVDLFILLIVAGAGD
                     ELQGIKRGIMEMADIVVVNKADGDNRQRAETTASEFRNAVHLFPVPASGFTPVVTTCS
                     SKEGTGIQEIWQTVNEYLAHARATGYFEKKRQEQSVRRMHDFILDYLENSFYNNPEIK
                     SSLQRVEEMLRRGEITSYKAAYILLNKFLNK"
     gene            complement(15120..16202)
                     /locus_tag="PLG42_01325"
     CDS             complement(15120..16202)
                     /locus_tag="PLG42_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU29881.1"
                     /translation="MKKLLIATICLLSGVLMLAQPKVQVATYEHDFGTFREEDGRQSY
                     NFEIVNTGNAPLVIQNVVASCGCTTPTWTKSPIPPNGKGIVTAMYDPAGRPGPFNKTL
                     TVYTNASPSTVVLVIKGEVKEREKTIEELFTFPVGKGVRFESNHLAFTTIKKTEKKIR
                     VMPVINTSNELAKIEFENVPGHLQLKAVPETLKPGQKGVIEGTYDATKNGNWGTVTDV
                     VRVKVNGVAQENTWFYVSANLVEDFSNLTKEELENAPVFKVQAATVDLGDMLPSTTKD
                     VEFKFTNEGKRDLIIRHIRSTCGCTAVNQSSSNIVKPGETGTIKATFNSGSYKGRVTK
                     AIYVYTNDPNHSEVILMLNANVIEKK"
     gene            16442..>18962
                     /locus_tag="PLG42_01330"
     CDS             16442..>18962
                     /locus_tag="PLG42_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29882.1"
                     /translation="MSGNFLKGLFFSLLLIYLPAGIRGQFYNGLQMSFGKNRVQYYDY
                     YWSFYRFENIDCYFKEEGRELAQFTANYAIKKLEEIEDYFDYNLEKRLIFIIFNKQAE
                     YKQSNIGLVTFSEDDYNLGGYSRIIKNKVMLYYEGNMVAYQKQIASSIVEVIVNEMIA
                     GADLRDRTTSSSSFDIPQWYMKGLANYAGYGWDYDVENKVKDGFKSGKYKNINHLEYD
                     DAIYAGQSFWRFIGRQYGDALIPSIIYLTKIYKNINDGFMYVTGRKLKDLLKEWQEFY
                     SKEFAEDNGDPPKDENNLKRSRKEQIFQQVKVSPDRRYVAYVTNDWGRKRIWVYDQQT
                     GKTKIIFRAEPKYEHKTDNTYPVIAWHPGSRILTFINEEKALLRIYFYRIQEKTTESR
                     LFLYLDKVLDFAYSPDGTKLVLSGVKGGFTDIYVHTIASGTNEQITHDLADDLSPSFI
                     NGSNDEIIFTSNRLSDTLTNTGNPFERLGLTYDLFLYNITKNDKVLTRLTEGKYNDHF
                     QPVNTGKDAFAFIGNNNGVYNRYSAVFDSTISFIDTIPHYRYFIKAEPLTDYDRNILY
                     HDVANNSDFFGEVVYNKSRYHVRKGNSSSGIKMKGKELPVTNFRKEHLNALHRADSIE
                     QLRQWLLLEDRKRRDTLKKPLYEYYAKDEPIDINHYIFEKEKQNYYEQLWRKDYMDID
                     LGTDTLQLPLIRIYQTSFYNNYIANQIDFSFLNNSYQPYNGGAPYFNPGTNLFTRIGI
                     LDLFEDYKITGGFRFAGNFDSNEYLLSVENLKGKFDKQLTYHRMTFNTSTEDYEYYKI
                     HSDNVYLTLARPFSPVLALKGTLSYRLDRYVNLSTDVASLSN"
BASE COUNT         5759 a         4088 c         3810 g         5305 t
ORIGIN      
        1 aaagttacaa cttttcaatc tgatagaata aaaaatcaga agccaaataa tgttaaaact
       61 gcaagggaaa gaagcaggat cagaaattat aaaagatacc tgatagtatt ccgaaagaat
      121 atggatgaac cattgaacca gtgatgccac ctaacggggt gatatagtat ctaagtaccg
      181 gttcgaggtt gaatgaaatg tttgttgtca gtttgtactc catacccata ccaactgaac
      241 tgctgaaagt gaccggactc aggccttcgg tatgtcctat tgtgtatttt aaaccacctg
      301 aatttatata tgatgtattt ccgatgagta taccgtatga gagtccgcca agcatgttta
      361 tatctacctt gcggtcaata agtttatatt tcatcatcag aggaatttca aggtaactca
      421 tgctctgaat caacgaattg cttaaatgac tcaggtttgc cttcatcgga tcaaaaatat
      481 cggctgaata tctggtctgc acacgtgcca cattactgtt atcaacaaaa tagatatcct
      541 tgtttgttga acttattgta ccggaagatg taagaattga aaaatcgctt gcacttttag
      601 attcgttata tttagagaat cctgtaaatg aggttattcc gtcaattctc tgtccaatag
      661 aactatagaa aatacctgat acaaatgaga gtctcctgct tgcctcaaaa gtaaaggata
      721 acccaccgga atatgagaat gccatttttt ctgatttcag ataatctgct gctgcatcgt
      781 catttccgaa actaaatctt gaatggtatg aaggcagaac agatgcacct actttccatt
      841 tttctccctc actggatcct ctttcttctg gtatgtaggt gatatcaaag gttttgcttt
      901 tcacttttcc gggaacatct aaatatctga gatttgcaag gttaagctca ttgtttgaag
      961 tcttgaaggt attgttttct gaaatcattg aagaaggtat tccggaaata tcaatgttat
     1021 tttcttcctt ctggctggtt tcctccatgg cagttacgac aggcatcctt attgctggtt
     1081 tttgcagctg agcagcaatt ccagagttat gttcagaggc taagaactga ccttcaggca
     1141 ttgcatcctg gtttaatgta agtgcaggag cgttagcaag tgtgtcagtg agtttattat
     1201 gtaaaaaata gactgccgtt ccggtgctga ggaccagggt ggcaaatgca gcagcacgca
     1261 taagggcagg atatattttc ctgcgcctct ccggtaacga cctgctaaca ttttcccata
     1321 catcttcagg gggcagtact tcatgatttt tcagtccgtt cctgaacacc aggtcaatat
     1381 ttgaatgcca gtcagccatt tataatagtc tttcttatac cagtatagag ttttaccttt
     1441 ttctgcagta tagttcttgc acgtgaaagg ttcgattttg atgttccttc tgatatattc
     1501 agcattttac tgatttcttt gtgtgaataa ccctcaatag catagagatt gaataccatt
     1561 ctgtattttg gtggcagttc cctgatcata taaagtagat ctgctgcatc gataccagta
     1621 taatcttctg tatcaggttc tgcctgaggt tcatcgatgg tatcaaggtc gtcaaccctg
     1681 tacaggttgt gttttgccct gtatttctcg agggcggtat tcacaacaat ctttctcagc
     1741 cagccttcaa aagaaccttc gtgtttataa ctatgcaggt tctggaatat tttaataaat
     1801 ccttcctgca aaatgtcgcg cgcttcttca tcatcactgg aatactggag acacactgca
     1861 taaagtttcg agcaatgtct ccggtataac agctcctgat cacgcctgtt accttccaga
     1921 caacctttta ttatttcttt tatatcgtcc aaaaactaac gtataacgat ataaaaataa
     1981 taaaaagtcc ggataaacat actgctccgg aataagtgat gaacaatttt tatgaaaggt
     2041 tgcgtcgggt cttagcaggt gctcatcata cctgcgggaa aaaacctttt acaaatttat
     2101 tgcctcaccg tatgctgctg cagcagcttc catgatggct tccgacaatg tcgggtgagg
     2161 atgaacagat tttattattt catatgctgt tgtttcaagc ttccgtgcta caataatttc
     2221 gccgatcatc tccgtaacat tctgccctat catatgagca ccgagcaatt caccatcctt
     2281 tgcattgaag ataagtttaa caaatccctc cctgtctcct gcagcacttg ccttacccga
     2341 tgcagtaaac gggaattttc caacctttat ctcatatccg gctctagcag cagctttttc
     2401 cgtcagtccg cacgaagcaa cctcaggcat agtgtaggtg catgaaggta tgttatcgta
     2461 gttgagaggt tcaacatcga gacctgccat tttttcaacg cagataattc cttcagctga
     2521 ggccacatgt gcaagggccg gacccttaac tatatctcct atggcatata tgccattcac
     2581 cgaggttctg taaaactcat ccaccatcac tttaccatcg tgggtcctga cacctgcttc
     2641 ctcaagcccg agattctctg tatttggtgt aatacccgcg gcagagagaa ccacatcagc
     2701 ttctattacc tggtttcctg aaggagtctt caccgtaaca tggcatttat taccagtagt
     2761 atcaactgag ctaacagttg agctggtcag gactttcatt ttcatctttt taaatgacct
     2821 ttcgagttgt tttgcaacct cctcatcttc aagtggcata atctgcgaca ttatttccac
     2881 aagtgtcact tcagtgccga gcgcttggta aaaacatgca aactcgcagc ctattgcgcc
     2941 tgaaccgaca ataaccatag aggcaggttg ttccggaagg gtcattgctt cacggtatcc
     3001 gataatcttt ttgccgtcta tctccatacc gggaagtgct tttggcctgg ctcctgttgc
     3061 tagaatgata ttagctgcag tatatacctt cctgttaccg gaatcatctg ttacctcaac
     3121 tttccctgca ccttttagtc tgcctgtacc cttaatatgt acaatattgt tctttttgaa
     3181 aagaaactgt atccccttgc tcatcccttc agctacagcc ctgctcctgg ctataattgc
     3241 cttaaagtcg ggtttaactt caccctgaac agatatcccg tattgtgagg catgcataaa
     3301 atactggtac acctgtgcac ttttaagtaa agccttggta gggatgcatc cccagttaag
     3361 gcatacccct ccgagttctg ccctttcaac tactgcaact ttcatcttaa gctgcgatgc
     3421 ccttatggca gcaacataac cgccaggtcc acttccgatt atcaacaaat cgtaatccat
     3481 aattctataa gtttattttt atttttacca ttgtaattat ttgataatta ataaattata
     3541 atcatttaat tatcagtatt ttactattaa catccaataa ctcttagcca gtaagtcaac
     3601 tgagttctgc caaaataact cgcgacataa gcattattcc atctcccgtt tcaaatttac
     3661 catagtaatc agttttaaga ttaaccacat aactaccaga acgaatatag ttgcagctcc
     3721 tgcaggtatt cctgtgaacc acgaaaagag ataacctccc gacgtgccag caaatccgaa
     3781 aagaaccgac agcttcatta ttgaagagta ttttcttgta aaaagcattg caatgttagg
     3841 gggaattgtt agaagtgaaa gtaccagaac gactcctgcc atccttatgt tcagaactat
     3901 cgtgagcgcc atcaacgagg ttgtgagata tttgaaaaaa tccacatatg atgaatatgt
     3961 tctggcgtaa ttttcatcaa atgatatata tagtattgtt ctgtaaaata ccgaaaagta
     4021 caataataga attactgaaa gaatgcccag ggcaataaga tcggcattag ttactgttag
     4081 aatgcttcca aagagatagc ttatcaggtt gggagtatag cctggagtca ggtaaatgaa
     4141 aatgatacca actgccatac ccaatgccca tataatgcct attgctgaat cttctcttat
     4201 tttctgtttc accgacaggt actcaatgcc tatggcggag aggataccaa aaacggctgc
     4261 tccggccaca ggattaacgc ctgcaaaata gccaatcccg attcctccaa atgaagcatg
     4321 agtgattccc ccgctcagaa aaaccattct ccttgagaca atatatgtcc cggcaatacc
     4381 tgctgtaatg cttgcaaaca ggcatcccaa tatacctttc agaataaaat catattgcaa
     4441 tacagggata tccattatca gtcgtgtttt ttcagaaccg tatgtggaac aggaccatgt
     4501 gtaacaagtt gaatcggaca gttataggcc gaaagctgtt cttcggtcaa ttccggagaa
     4561 gggtgataat gcagatgcct gttgacacat gcatacgact tgacatggta ggaaattgta
     4621 ccgacatcgt gtgatgccat cagaattgcc atacggctat tcagttcgtg aagttttata
     4681 tagaactcat tttcaaaggt ggtatcaaca aagttgtcgg gttcatcaag taatagcagc
     4741 cggggattgc ctataagtgc ccttccaaga aaaactctct gtatctggcc tcccgacagc
     4801 tcagcaagtg tcgccttctc aaaacccttc aagcccattt catcaattat cccgtctgcc
     4861 tttttcctgt cttccctgtt cattttaaca taaacacctt tgtgcatcat aagtcccgaa
     4921 agaataacgt cagtgacggt aagagggaaa gaaatatcaa ctgatgaaag ttgcggcaga
     4981 tagccaatat ctgtgcgttt tagcatctca tgattataaa ttacagtacc cttcattggt
     5041 ttaaccattc ccagaatgac cttcagcaga gtagttttcc cgcctccgtt tgggcctata
     5101 atacctataa aatcttcctc atatacggta agattcacat cttccaatac cacctttgaa
     5161 ttgtatccgg ccgtaacgtc aattagttct acaagttttt tcattttttc gaatcactgg
     5221 cagtaaaact ttcatagatt gcgtcaatta tatcattaac agcgccgtac caatcggccg
     5281 atagtggtct tatttccctt ataccagcac ccgattccga tgcaacaacc cttgcatttc
     5341 ttgtatcgta ttctttctga atcagtatca ctcctgtcct gtttttcttt gccatatcaa
     5401 taacttctct cagccatccc ggcgatggtt ccttgccttc tttctctatc gaaacctgta
     5461 tgaggttata atccttcgca atgtaagcaa gagtatgatg aaaaacaaca aaggagtgtc
     5521 cggcaaaggg ggcaaaaagc tcggaagcac gcttatgtat tttaaaaatg gaatcttcta
     5581 cagcgtgaaa attcaaagta tatttttcac agctatcggg tttaagttca caaaggagcg
     5641 attttactga ttcagctatt ttcaaccctg aaaggggtgc cacccagaag tggggatcgg
     5701 catacaacat aattccttca tttacctcag tctcttcatt aataagctca acactgtcgg
     5761 caagttttaa tttcttcatt gaaggattta cagaatagaa cttatcgagc catgctaatt
     5821 cgaaatcgag ataaccgtcg cttatatatg ctgacgattt tcgaagagct gcaacctgtg
     5881 ccggcgccgg ctcgtaaaca tgagggtctg ctccctcagg taccataaca tttacaatga
     5941 aatcaccccc tccaatcttt tcaacaaaaa acccaaaagg tgctatgctt accgtaatca
     6001 ggcctttatc agtagaaaaa gtatttggct tacaggacaa tagtgacaat aaaatcaaaa
     6061 aaagaaactc tttgcgcata aaaatgtgtg ttcaagggta caaaattaac acgttaaagg
     6121 gtgaaaagtt taaatattgg aaataaaata ataaaaatta attttgatat tttctaatta
     6181 agtctcaccc gcaatatatg aaaaacaagg gagaaatact tgcagaaatt ctgagggaat
     6241 atgatttttg ctatttcttc ctccataaca caagagacat aaacacagtc aataaaatcc
     6301 tgaatgaagg cttccggtac gaaagccagc ttccgcattc aaccgacagg gtgaatccac
     6361 atgagccaat cgagattgtc tattttctgt ttcagagaaa agaatacgga ccgtacaccg
     6421 tggtaattgc cataccaagg gaaacttacg agagatacac acgtctttcc aatattctcg
     6481 acactaccat tgaggaggtg atgagcacag agaagcccta ttacagtgag aacgacgagc
     6541 ttgtatacac gatatctcca aagcacatac tcggatactt taacaacaat acatcagaat
     6601 ttgtaaaaaa tgagcactgg gatcctcttt ttgatatttc acaactgaag acattcaaaa
     6661 ggaggccacc tgccgatgcc aaaccgaagt aattatcctt tacctctacc aggtaaaata
     6721 caatcattat ttgcattatc ttgcaggggg ctacccccaa accccctggc agggggctac
     6781 ccccaaaccc cctggcaggg ggctaccccc aaaccccctg agaggggact acccccaaac
     6841 cccctgagag ggggcttgaa tgtcattcat ttctaaatta ttagctccgt aggagcgcca
     6901 tgtttgtagc aatggattca gacaattttt ggcgccggaa cgagaagttt atttgaagat
     6961 gaatcttcat acccggcgca attggttttg aatcgtttaa atccccgcct cactgccgga
     7021 aagcagcatg gtgggcaagg gtgtgatatt aatttatagt catttgcgtt tcgctccgct
     7081 caacgcgtaa ttcaatagta attcgatcca ttgatctata tgacctgaac ccctatattg
     7141 ccaaatggca caaatatagg ggggttgggg ggtgatttca atcgttcgct ttgccatggc
     7201 tcggttcttt cagcaagcaa agaaagtaag agtaaatatt cttagactct tggaattaaa
     7261 ctaaaaacca tctcagatgt taaaaaatga taaaaaatac ccttaatttt aaaagtttct
     7321 ttgcttaata attttaatga aataatttca actcgctaaa aacttaaaaa actcaacatg
     7381 gttaaacgtc ttttaaaatt tacatttctg ctggtagtta tttccagcat ttttatttca
     7441 gagagtcttg ctcaatttgc aaaccacagg gctctttcac agaaagcaac aaagctggca
     7501 acagattatc ccgccctctg ctcggtcaaa tcacttgtca agacacccgg aggcagggat
     7561 gtatgggtac ttgccatagg aacaggcgaa agggacagca aacctgccat tgccattgtg
     7621 ggaggaatcg acggtagtta catattcaca agggaactgg caataggttt cgctgaaaat
     7681 atcctgaaag aatctgccac accagaaatt aaaaaccttc ttgacaggat cactttttat
     7741 gtctttcccg acgtcaatcc ggatgcttcg gagcaatatt tttcaacgct cagatatgaa
     7801 aggcttatta acgacaggcc tactgacgat gaccgagatt ttcagacggg ggaagatcct
     7861 ttcgaagacc ttaacggtga cggcctgatt acacttttaa gggtatctga cccggcaggt
     7921 aaatatatcg aaagtaccga cgataagaga gttatggttg aagccgacct ctcgaaaggg
     7981 caaaaaggaa cttatctttt attcagtgaa ggtatcgaca acgataaaga tggtcttttc
     8041 aatgaagatg gtgcgggtgg agtggacttc aacaaaaact ttacatacaa ctacgaggaa
     8101 tacggagcaa atgcaggttt gcatgccgtt tcagagcctg aaagcaaggc tgttgcagac
     8161 ttcctttacg accatttcaa catatatgca gttatctgtt tcggacccca ggataacctt
     8221 tcgcagcaga ctccaagagg aaatgagcga cctgcacctt ccgggcagca aacccaggca
     8281 atccagcagc aacaaggaag gcaggcaggc gaaaggagaa taacttcagt gttgagaagc
     8341 gacgaaacag ttatcaggct ggtttccgaa aaatttcagg aaataacagg cctcaggggt
     8401 gcccctccat caaagacctc tccgggaaac tttgccgact gggtatatta ccactacggc
     8461 cgctactgtt ttaccacacc cggctggtgg tttccctccg aaagagggaa aaatacagaa
     8521 gtatcattcc ttaaatttgc cgaagacaat aaactcgaaa atgtcttcgt gccatggacc
     8581 gaaataaaac acccggattt cccggataaa aaagttgaag tgggtggcat aaaaccattc
     8641 gcaatgctta ctcccccagc agataaagta caggaaatga taacaaaaaa ttacaaattt
     8701 atcgttgaag tcgcaggaat gcatcccgaa cttgaattta tagacaaaaa ggttgagaat
     8761 gtcggaagcg acatttaccg gattaccctg aaagttcaca acaaaggact cttcgccacg
     8821 tgctccgaag caggagccaa taaccagtgg acaaggataa tgaggatttc acttgaaccg
     8881 gcaaaaggac agacaatcct aagcggccag aaggtacaga ggatgcagag gctagaagga
     8941 aacagttcct cagagttctc gtggctgata tcaggtaaag gacgtgttgc tatatctgca
     9001 ggtgcactga atacgggaac aataagcaca tctttcgatc tgaaataaaa atttaaaaca
     9061 gacaggaata tgaaaagcat aaagaaaata tcattaataa ttcttctgat ggttctgaca
     9121 tcagcagcat caaacgcgca gaaagaggag ctctttttca gggctgcagg ttcaccacct
     9181 gatcctaagg tacaggcttc atggaataaa tattacacaa atgccggtat atatgacctg
     9241 tgcagcaaac ttgcaaaagc atatcccaac cttgtttctc tagagtcaat tggcaaatcg
     9301 taccagggaa gggatatctg gctgctgaca atatcggata aaaaagcagg caatcccgac
     9361 aaaaagccag gttactggat tgacggcaac attcactcaa ttgagctgca gggaacggag
     9421 atggcacttt acacggcctg gtatctttgc gagatgtata acaccaacac attcatcaaa
     9481 gagttacttg ccgaacggac gttctacata gcaccaacca tcaaccctga tgccagggaa
     9541 tattttacat cggtaggtgt tccgccacgc tccggccttg ctccacgtga caacgaccgc
     9601 gatggtctct ttgacgagga tggtttcgat gacctgaacg gtgatggcga aataagccag
     9661 atgagaagga aaaaccctga aggaaattat atagttgatc caaaagacaa cagaagaatg
     9721 ataagggttg agccggggca gaaaggcgaa tatgagttac tcgggcagga aggcattgac
     9781 aacgacggtg acggtctggt taacgaagac ggcccgggtg gttacgacgg caatcgcgac
     9841 tgggggttca actgggaacc caactatatc cagagcggag ccgacaaata tccgttcacc
     9901 tttcctgaga atcaggctgt gagggatttt ctgctgaaac acagaaatat tgcaggagct
     9961 cagtcatttc ataactcggg tggcatgatt ctccgggggc cctcggtaca aagcggttat
    10021 gccgaggcat acagcagggc cgacgatgca gttatagaag cactcggtaa gacaggtgaa
    10081 aagatacttc cagggtacag gttgcttaca gtttggaaag acctgtatac ggtttatggc
    10141 ggtgaagttg actggatgca tggagcaatg gggtgtttcg tattctcaaa cgaactctgg
    10201 accccatacc tcatgttcta tgacacaacc aataccgacc agtatgaatt tgacaggctg
    10261 ctactctttg aagatgcatt tataccctgg cagaaaataa accatcccgt ttacggagag
    10321 gttgaaatag ggggcttcag caagaattac ggaaggctgc atccgggttt tctgctagaa
    10381 tcggatgcgc atcgcaatgc tgcattctgt atatataacg catatcacct gcctaaactt
    10441 gaggttacaa aagtaacagt gaaaagcctt ggcggaggac tgaaagaggt ctcagcaacc
    10501 attgaaaaca aaaggatggt tccaacacac tcggccagca acctgaggta caaaattgac
    10561 cctcctgatt atgtttacat cgaaggggca cagagcgtac ttgcaggcat gatagttgaa
    10621 aacgaagacc tcaacatatg cagggaacag aagaaaaatc cggcacggat cgaaattgag
    10681 aacatacccg gttatctggg cggtggcgca caaacgggag ggggaggatt tgccggagga
    10741 cgatcccctg gagcagccgg caacagcaac attattaaag tgaaatggat tgttaaagga
    10801 ggtgacaggt acaccgtaag ggttgaaagc gttaaaggag gtacagcttc tgcacaatca
    10861 aactgatatt ttcaaactga aagaaaaagt cgttcacggc aaatgcacgg aacgactttt
    10921 ttatcttact attcaggaaa ttatttcaga atgtttgcac tgaaatcctg aaatagcctg
    10981 ttgctttcgt agtttcaact ttaaatgtcc actcacctat cttgtcctta ttttcttctc
    11041 ctgaaacagt aaatgatttt ctccagttaa gatttcctga agtatcaatc agaacttctg
    11101 catatgtttt accatcaggc gaaaggattt tcaccttaat ctcaccggtt ttgcaatcac
    11161 cgctgatact catcaccaca ttttttgcat cactgtccac ttcaaaagga agctgccttg
    11221 taattgtggc ttcgttcagt ccttttgaaa actcgaatga tgtaccgctg cctctgaaat
    11281 aacttaatcc gccaaaatca cctcccggtc caatataaaa tgcgcctgca tcggctcctt
    11341 ccctgccaat aaaaacattc gttctgcttc tccttgaagg gtcgtcagga cgaacacctt
    11401 caggtctacc ctgttcctgt acaacagcct tctgtatctc accagcctgc tgttccataa
    11461 tctttgcttg ctcctgcatg gcttttttct gctgttcaat agcctgctga agcctggctt
    11521 ccttctcctt ttgctcctgg gttttctcct gtcctgccgc ccctgcaacc accattccca
    11581 gtatcagagc cgaaagcact cctgtatata aaactttgag tttcatgatt tcctccttta
    11641 attactttaa ccactgtaaa aataagcaga taatgtacca ctttttgtta atgccgggtt
    11701 aatgtaaggt taatcttcaa ccatatctta gcaattaatg aaataaattc aataatttta
    11761 atgtcgattt atatacaatg agattaaaga acagcatact ggttgccgga ataatttcaa
    11821 tagttatcct tattatcgta caggtactgg taataaagga tatctggttc gacaaggatg
    11881 taattttcga cctgaaatac aagcttctct ctcaggaggc tatggagcag ctaaacagga
    11941 tgagccagaa cgaccctttc gaaaatgcat tgaatttcct taacgactat tcccggcagg
    12001 tgatgacaac tgaaatacag aatgtcaaga atgatgaaga cctgactcgt cttaaggaag
    12061 agattatgag ggatgtcaat aaaatattga ctgaactcga aaacctcagc ccttatctta
    12121 aaggttattt caacaaacag ggatttgaaa gtgatttctc aatcaagata gaagtaaatt
    12181 ttctccggct gatagatttt gagaaaagat tcgatgttta tgcaaacccg cagatgtttg
    12241 caggaccacc tgtttcgtcg gcaaatgtta caaaagatac gcgttcaaaa atacttgtaa
    12301 accgcttttt cagggaagat aattactacc gcatcgcttt tgattattac atagatatat
    12361 caggaaagca aaggatggtc ctccgggata tggcactata cctggcattg agcatcttca
    12421 gtataattgt tgtaatgata atattttata tcacttacca caatctgatg gaagaaaaga
    12481 ggctttcgaa cctgaaaacc gatttcatta acaacatgac ccatgagatc aagactcctc
    12541 tttcaaccat aacggttgct ggtaaaacgc tggaaatgga gcaaataaga acaaatgaaa
    12601 ataaggtgct tgaaactgca aggctaattc ataagcagag cgtacatcta aaccagctca
    12661 ttaatctgat tctggaaatc agcatgtggg aaagaaccca gtttcagctc gacaaaaagg
    12721 aggtggacct cagggaactt atgcacgata tcgttgagag tttcagaaca gcacaaaaca
    12781 acaatgttca gattatagaa aactacggct tcgacactct gaaaacaaag ctggatgttg
    12841 tatatttcac taccatgttg aataacctgc tgtcaaatgc agtaaaatac tccgataaag
    12901 aacctgtaat aaaggttgaa ggacaggtga ataaagaaaa tattgttatt tcggtaacag
    12961 acaatggtat cggaataagc aagcaggacc agaaacatat atttgacaaa ttttaccgtg
    13021 tgagccatgg caatattcat aaggtaaaag gacttggact tgggctttat tacgtaaaga
    13081 ggattgcaga ggctcatggc ggtactgtta cagtatcaag caaacccggt gagggcagtt
    13141 gttttacagt tacaattccg aaataattaa aaacgagcga tatgaaagta cttattgcag
    13201 aagatgacag ggatttcggg aacgtacttg cgcagtatgt cacaataagc gggtttgagg
    13261 tcactctcgc ccgcgatggt aaagaggcct gggagctctt caataaggat cgccccgata
    13321 tctgcgtact cgatgttatg atgcctgaga tggacggatt tacgcttgcc gaaaaaatta
    13381 aagaggcagc accggaaatg ccttttatct tcctcactgc aaaaagcctg aaggaagaca
    13441 tagttagggg tctgaagctg ggagctgatg actatattac aaaaccattt gatcctgaag
    13501 ttcttattct cagaattaat aacattctga aaagagctta ttcgtcggtg aatgatgaat
    13561 acaaaatctc tgacacaatt ctgaaattca acacacttga gctaatttca ggtaaaaaga
    13621 aagagaaact tactttgaag gaagcccagc tgctgaggta tttcataatg aacaaaaata
    13681 agatactcac ccgtgaagat atcctgacag agatatgggg cgaagacgat tatttcctgg
    13741 gaagaagcat ggatgttttt ataagcaggc tccgtaaata tctttcggag gataaaggca
    13801 tcgatattcg cacagttagg ggcacaggtt ttattctgga agagctcgga agcggcatgc
    13861 aggagaaaga tgaagaagag aagacagagc ctgaaatgtg acagcataaa ctacttgttc
    13921 aggaatttgt taagaagaat ataagctgct ttgtatgaag taatttcacc tctgcgaagc
    13981 atttcctcta cccgctggag tgatgactta atttcaggat tattgtaaaa tgagttttca
    14041 aggtaatcca gaatgaaatc atgcattctt ctgactgatt gttcctgtct tttcttttca
    14101 aaatagccag tagctctggc atgagccaga tactcattta cagtttgcca tatttcctgt
    14161 attccggtgc cttcttttga tgaacatgtt gtaacaaccg gagtgaatcc ggaagctggc
    14221 accgggaaca ggtgcactgc atttctgaat tcacttgcag tagtttcggc tctctgcctg
    14281 ttgtctccat cggccttgtt taccacaact atatcagcca tctccattat accgcgtttg
    14341 atgccctgca attcgtcacc ggcacctgcg actatcagaa gaataaaaag gtcaaccatc
    14401 gatgcaactg ctgtctccga ttgaccaact cccactgttt cgatcaatat tgtatcaaat
    14461 cctgcagctt cgcacaatat tatactctct cgcgtcttgc gagctacacc tccaagggtg
    14521 cctgctgagg gcgaaggcct tataaaagca tttgggtcgg tgctcagacg ctccattctt
    14581 gttttgtcac caagaatact gcccttcgtc tttttgctgc tagggtcaat agccagtacg
    14641 gcaacttttt tgcccatacc ggtaagcatc gttccaaaag attctatgaa tgtacttttg
    14701 cctgcacccg gtatgccagt tatgccaatc cttactgagt tgccactgta ttgcagacaa
    14761 ccctctacaa cctcctgagc aagactgtaa tgattggggt tggaactctc aataagagtt
    14821 atagcctttg ccagcactgc cctgttcccg gaaagaatac ctgatatata ttcaccggca
    14881 gaaagaattt tccttttccc tgcaatattt tttttcagaa tctcaggatt caccaatggg
    14941 ggctgttcaa taccatttct tatactgagt gcactcccct ttttctgttt atcaggcatc
    15001 tgtaccataa attgctgcag gtaaatttaa aaaataaaaa agcgcctttt tgttaaggcg
    15061 ccttttccct gattattttt cgacatctta ctcagattct gaatacagac tggtaattct
    15121 tatttctttt ctattacatt ggcattgagc atcagtatca cttcagaatg attcgggtcg
    15181 ttggtataaa catatatggc ttttgtaacc cttcctttgt atgaacccga attgaaagtg
    15241 gcttttattg tacctgtttc accaggttta acaatgttgc tgctgctctg gttaaccgca
    15301 gtacatccgc atgttgaacg tatgtgcctt attatcagat ctcttttacc ctcattggta
    15361 aatttgaatt caacatcctt tgttgttgag ggaagcatat cgccaagatc aaccgtagct
    15421 gcctgaacct tgaatacggg agcattctca agctcttcct ttgtcaggtt cgaaaagtct
    15481 tcaaccagat tggcagaaac ataaaaccat gtattttcct gagcaacacc gttaaccttg
    15541 actctgacaa catcagtaac cgtaccccag ttaccattct ttgttgcatc ataagttccc
    15601 tctataacgc ccttttgccc gggcttaaga gtttcaggga ctgctttcag ctgcaggtga
    15661 cctggcacat tctcgaattc aatctttgcc agttcattcg atgtatttat aacaggcatc
    15721 acccttatct tcttttctgt tttcttaatc gttgtaaaag caaggtgatt gctttcgaac
    15781 ctcactcctt tgccaacagg gaaagtaaag agctcttcaa tggtcttttc cctttcctta
    15841 acctcacctt taattacaag caccaccgta gaagggcttg cattggtata aacagtgagt
    15901 gtcttgttaa acggaccagg acgtcctgca ggatcataca tcgcagtaac tatacccttt
    15961 ccgttgggag gaatgggact tttagtccat gtaggtgttg tacaaccgca cgaagcaaca
    16021 acattctgaa ttaccagagg agcattacca gtattaacaa tctcaaaatt atacgactgc
    16081 cttccatctt cttccctgaa ggtgccaaaa tcatgctcat aagttgcaac ctgcactttg
    16141 ggctgtgcca gcatcaaaac acctgataac aaacaaatag tggcaataag aagctttttc
    16201 atatcatttt attttattag taattcaaac ttaatcaaac acctgattta tatataacct
    16261 gaattatcgt catcttgttg catactccat acaaaaagca gaccattttc cgttaaattt
    16321 ccaaaattaa taaagtttac tcaaatttat aaaagcaggt aaagattata ttattagatt
    16381 tgtcaggatt gttttggaac agaatttgaa aagagatttg acggcaaatt tcatcaggct
    16441 gatgtcaggg aattttctga aagggttatt tttctcgtta cttctgattt atctgccggc
    16501 aggtatcagg ggccagtttt acaacgggct tcagatgtca tttggcaaga acagagttca
    16561 gtactatgac tattactggt cgttttaccg tttcgaaaac atcgactgtt attttaagga
    16621 agaaggaagg gagctggccc agtttactgc caattatgct ataaaaaagc ttgaagagat
    16681 agaagattac ttcgactaca atctggaaaa aaggcttatt ttcataattt tcaacaagca
    16741 ggcagagtac aagcagtcaa atataggcct tgttacattt tcagaggatg actacaacct
    16801 cggagggtat agcaggataa ttaagaacaa agttatgctg tactacgagg gaaatatggt
    16861 tgcataccag aaacagatag cctcatcaat tgtagaagtc attgttaatg aaatgattgc
    16921 aggggcagac ctgcgcgacc gaacaacaag ttccagctct ttcgacatac cccagtggta
    16981 tatgaaggga ctggcaaact atgctggcta cggatgggat tatgacgtgg aaaacaaagt
    17041 aaaggatgga tttaagagtg ggaaatacaa aaacataaat caccttgaat atgatgatgc
    17101 catctatgca ggtcaatcgt tctggagatt cataggccgg caatacggag atgctctaat
    17161 tcccagtatt atatatctga ctaaaattta caagaatatc aatgacggct tcatgtacgt
    17221 tactggccgt aagctcaaag atttgctcaa agaatggcag gaattctaca gtaaagaatt
    17281 tgcagaagac aacggcgacc cgccaaaaga tgagaataat ctgaaaagat ccagaaaaga
    17341 acagattttc cagcaggtaa aagtcagtcc cgacagaaga tatgttgcat atgttacaaa
    17401 cgactggggc agaaaaagaa tatgggttta cgatcagcag actggtaaaa cgaagataat
    17461 attcagggcc gagcccaaat atgaacacaa aacggataat acctatccgg taatagcctg
    17521 gcatccagga agcaggatac ttacatttat caacgaagaa aaagcccttc ttagaattta
    17581 tttttaccgg atacaggaaa aaacaaccga aagcaggctc tttctctacc tcgacaaagt
    17641 tcttgatttt gcctactccc ctgatggcac aaaacttgtg ctttcgggtg ttaaaggtgg
    17701 ttttaccgac atttatgtcc ataccatagc ttccggaaca aatgagcaga taacccatga
    17761 ccttgctgac gaccttagcc cgtcgtttat aaacggctca aacgatgaga taatcttcac
    17821 ttcgaaccgg ctctctgata cacttacaaa caccggcaat ccttttgaac gtctcgggct
    17881 cacatacgac ctgttcctgt ataatataac taaaaatgat aaggttctca caaggctcac
    17941 cgaaggaaaa tataatgacc acttccagcc ggtaaataca gggaaagacg ctttcgcatt
    18001 catcggaaac aataatggag tttacaacag gtattctgca gtttttgaca gcacaataag
    18061 ttttatcgat actattcctc attaccggta tttcatcaag gcggaacctc tcaccgacta
    18121 cgaccggaac attctttacc acgatgtggc aaacaattcc gactttttcg gagaagtggt
    18181 ctataacaaa agccgatatc atgtacgaaa gggaaactca tcttccggca ttaagatgaa
    18241 aggaaaagaa ttacctgtaa caaacttcag aaaggagcac ctgaatgctc ttcaccgtgc
    18301 cgacagcata gaacagctga ggcaatggct tttgcttgaa gaccggaaga gaagagatac
    18361 tcttaaaaaa ccactttatg aatattatgc aaaagacgaa cctatcgata tcaaccatta
    18421 tattttcgaa aaggagaaac agaattacta tgaacagttg tggcgaaaag attacatgga
    18481 catcgacctt ggcaccgaca cccttcaact gcctcttata agaatatacc agacatcatt
    18541 ctataacaat tacattgcaa accagatcga tttcagcttt ctgaataatt cataccagcc
    18601 ttacaacggt ggtgctcctt acttcaatcc tggcaccaat ctatttacac gtatcggtat
    18661 tctggatctg tttgaagact ataaaattac aggtggattc aggtttgcgg gcaatttcga
    18721 cagcaacgaa tatctgctca gcgttgaaaa tctgaagggg aaattcgaca aacaacttac
    18781 ctaccaccga atgacattta acacttccac ggaagattac gaatattaca aaatacacag
    18841 cgacaatgtt tatctgactc tcgccagacc tttttcacct gtactggcat tgaagggaac
    18901 attgtcgtac aggctcgaca ggtatgtaaa cctttcaact gatgtggcat ctctaagcaa
    18961 cg
//
LOCUS       DAOSNB010000013        18150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3097_length_18150_cov_4.668914, whole genome shotgun sequence.
ACCESSION   DAOSNB010000013 DAOSNB010000000
VERSION     DAOSNB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18150
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3097_length_18150_cov_4.668914"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLG42_01335"
     CDS             <1..736
                     /locus_tag="PLG42_01335"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HOU29883.1"
                     /translation="LILGAEPDISVAVKCRLKVFINPNVPLIYSDTVTINVKPFEKVI
                     EYPKLYVPGSYQGWVLNQSTPVITSAKSDGKYEGYLYFPDANTEFKLTKVPAWEEPNT
                     IGDPDPSGMSGTLKIGGWGGNNIKVTGGPGQFRINADLNEATYSWVKTTWALIGDATP
                     GGWSTDTPMTYNINENTWTVTVNLIGGKSFKFRANGEWAINLGDTGGDRKLEYNGDNI
                     PVAENGNYTITLDLSKTIYKYKIVKN"
     gene            833..2005
                     /locus_tag="PLG42_01340"
     CDS             833..2005
                     /locus_tag="PLG42_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOU29884.1"
                     /translation="MLNRFTYSVILFVILIVLTCCKRQGDSLQQKRIYYLPDKFVMGA
                     DLSYVNQILEHGGVYRDSGTIKDPYLIFRQYGTNVVRFRLFHNPVWTREVYSPPSEKM
                     YNDFADVKDGIRRAKLLGMKVCLDFHYSDTWADPSKQIKPAAWQSLDIAALRDSVYNY
                     TYKTLKELDKEGLMPEYVQPGNEINPGFLLPEGSRWNGNENNMIYLLNGAISAIRSAG
                     NSSQIKPRIIIHVAQPENADPWFEGLWQKGLTDYDFIGLSYYYIWSKVKLEELSNYIS
                     MLKTKYTKDVMIMETAYPWTIENADNYPNIIDTGKLTDRFPATQEGQFNYLKTLVQEI
                     IEGGGEGVFYWEPAWITSEMKDLWGTGSSWDCNTFFDFQGNTIKGINYMTEKYNIR"
     gene            2108..4825
                     /locus_tag="PLG42_01345"
     CDS             2108..4825
                     /locus_tag="PLG42_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU29885.1"
                     /translation="MISTDPAVPTLGRVIKIYYDTNQDAGELHNYTGDIYVHTGLILE
                     GSPNWQKVIGTWGNNAVQPKLNYLGNYKYEFIISPSIETFYNVLPGEKVKKIALVFRN
                     SDGTRQTRPDIFIDVFESGLNVIFTLPERFSVVIEKNSLLGVSASATNADSISLYINN
                     QYRKSGRTRDLVTDTIIAESYGEYWVKAVAWDLPRFAADSFFCHVRKSVEVEELPPGS
                     IDGINYINDTSVILVLHAPYKNYVFATGDFTDWLACSKGYMKRTPDSERYWVRIDGLE
                     PKKEYRFQYFVDGELYIADPYADKILDPFNDDFITESTYPGLIKYPKGITNGMVSVLQ
                     TAQNPYKWKNEFFNAPPPEKLVIYELLLRDFVASHDFKTLTDTLNYLKNLGINAVELM
                     PVTEFEGNLSWGYNVAFYFAPDKYYGPKEYFKAFVDSCHGKGIAVIMDLVLNHCFGQS
                     PFVQLYLDHYGAQEIVMKTPNPWFNSVSPNPVYKWGADFNHEAPATQKLVDRITSYWL
                     TEYKIDGFRFDFTKGFTNTPGEGWTYDASRIAILKRIAGKIWEVNPSAYVILEHFTEN
                     AEEKELAEYGMLIWGNLNYQFAEAVMGYDSDLSGATHIARGWKVPNLVSYMESHDEER
                     IMYKALAYGASAPDYNVKNLQTALRRMELAAVFLLSIPGPKMIWQFGELGYDISIDYN
                     GRTGEKPLLWNYFSIPERRRLYRVYSVMNNLRQTCDAFSNTFFTYSLSGKQKSIILGS
                     QNIKAYIVGNFDISTATFIPLFPQTGKWYEYFSDDSLTVNSFSDPLTLRPGEYRIYTT
                     VRLPSPKEYLGVQEILDNELISVNAYPNPSKDQFYFTVNTKLPGLLTLTIYNSTGNTI
                     WQKKMPALNEFIVEWDGKDSRGNDIPAGIYFVHVRNGKYYNVIKIVKLN"
     gene            complement(4822..6699)
                     /locus_tag="PLG42_01350"
     CDS             complement(4822..6699)
                     /locus_tag="PLG42_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOU29886.1"
                     /translation="MGIQNFYKIIAILLLACGCNYKTTYTVPEWSKKAVWYQIFVERF
                     RNGDPSNDPSVKDITGAYPDNYPEDWSITPWGHDWYAHEPWLDKIKGGNFHAKIQARR
                     YGGDLQGVLDKIDYLDSLGITAIYFNPLNDSPSLHKYDARTYTHIDRNFGPDPEGDSQ
                     LMESETPDDPATWKWTSADRLFLKVVEEFHKRGIKVIIDYSWNHTGKEFWALKDIREK
                     GPFSKYADWYNIISYDNPATPENEFRYEGWGGRNIYMPVFRKDIIPPDDTIMPFEGNL
                     HSSSLRKHIFDVTRRWLDPDGNGDPSDGVDGFRLDVAAEIPMGFWREYRKVVREINPE
                     AYLVGEIWWLKWPDQLLDPAPFMGEQFDAIMNYRWFRVARGFFAQAEPVLTPSEFVEW
                     IKKLNVGIPAENQMAMMNVAGTHDSPRLSTSFYNKTLDKYRAKPSENPDYKINKPDSI
                     TLLEQKMFLVHQFTFTGAPHIWYGEEVGMWGADDPDCRKPMVWNDIVYEVERTHFYPD
                     KIRPADTVKPDHNLNEFYKKMIKLRKENPVLVYGDLDFLIADDNKMILAYSREKGSEE
                     IITIFNRSGSEQTVMLPVRKKALYIELFPDNKKDYKTNNKNLIINIKPLSAIVLKRVG
                     T"
     gene            complement(6672..8051)
                     /locus_tag="PLG42_01355"
     CDS             complement(6672..8051)
                     /locus_tag="PLG42_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOU29887.1"
                     /translation="MEKIILYVLSIFFVAALAAGGCLHKRNRNNYQPFVSEVVHPEWS
                     KDAVLYEVNIRQFTPEGTFKAFTQHLPRLKELGVDILWLMPIHPIGIKNRKDELGSYY
                     SVRDYMKVNPEFGTLEDFKILLDKAHEAGFHVIMDWVPNHSSWDNPLTTEHPEFYVKD
                     KDGNFIPPLGTDWTDVIQFDWTNKGLHEYMLNALKFWVELGVDGFRVDHPHKTPPEFW
                     ETVRAELWKIKPVLLLAENEGQNEFMKKGFDMNYSWELHHLMNQIARKKDSVNSLEKY
                     YEKELFVYPQNTYRMVFLDNHDENSWNGTITSRMGEAQYPFAVFMFTTRGVPLLYNGQ
                     EVCLNKSLRFFTKDTIRWDTCTMTAFYKKLIRLKKENQALWNGEEGGKMEIIKTNQPG
                     KVFAFSREKDGNLLIVFLNLRDKEVAVKPVLKNIRGTFTDYFNHSVIEMPDDKPLIIE
                     PWGFRIFIK"
     gene            complement(8099..10417)
                     /locus_tag="PLG42_01360"
     CDS             complement(8099..10417)
                     /locus_tag="PLG42_01360"
                     /inference="COORDINATES: protein motif:HMM:NF010380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOU29888.1"
                     /translation="MIKTNLFKIHEWTIEEEGFHPEINREAESIFSIGNGYMGHRANF
                     EEKYSGDSLQGSYVAGIYYPDRTKVGWWKVGYPEYYAKIPNSPKYIGIDVNVNGAEID
                     LAKHKPLKFNRVLNMKEGFLERNFTVRPFSRKEIRIQSLRFLSMADPDLCIIKYKINP
                     VNLSGTLSLNIYLDADVKNENTNYGEKFWEDIDNLAEPGLGMLTARTLRTDFYVTVGM
                     TYELTLNGNKLNLVPEVINRKGYAANIIHLDFQPGDEIVIVKYVSVQSSLYHPVKDHF
                     KITTSRLNYAKKKGFENLFSAHKKEWENIWSISDVKIDGDPAAQQGIRFSIFHLNQTY
                     TGKDERLNIGPKGFTGEKYGGSTYWDTEAFVLPFFLKTAPAYVSKNLLLYRYRHLKKS
                     IENARKLGFRGGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAYAIFNYIRHTGDKD
                     YMKDYGLEVLIGISRFWSQRVSFSKNLRKYVILGVTGPNEYENNVNNNWYTNSIAVWT
                     LSYTIHNIEELKKSNPAALEKIFLKTKFRYKSETGKWKKIIDGMHFPYDPKRKIFLQQ
                     DGFLDKELKPASAIPVSNRPINQHWSWDRILRSCYIKQADVLQGLYFFEDQYDTETIK
                     RNFEFYEPMTVHESSLSAGVHSILAARIGNIDKAYELFIRSTRLDLDDYNKEVAEGLH
                     ITSMAGNWLAIAEGFGGMRIAGDRINFNPLIPDKWKSYSFHARFKEILFEIKVTKSFC
                     IVRNLSERILPISVKEKNFDISPKDSIKIKTG"
     gene            complement(10414..11076)
                     /gene="pgmB"
                     /locus_tag="PLG42_01365"
     CDS             complement(10414..11076)
                     /gene="pgmB"
                     /locus_tag="PLG42_01365"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOU29889.1"
                     /translation="MWFDACIFDLDGVIVDTAKYHFLAWKRLADMLGIPFSEKENEKL
                     KGVSRLASLEIILELSGKKYTDSEKAKFAEMKNTWYVEFVSRMTPSEILPGAIELLTD
                     LKSHGIKTAIGSASKNTLLILEKVRMKDLFDTITDGNMVKSAKPDPEIFLVTASILNV
                     KPEKCIVFEDSVAGIKAAHKAGMYGIGVGNKEILIEADYVINDLSEMSFLKLTALKTI
                     NQ"
     gene            complement(11090..12592)
                     /locus_tag="PLG42_01370"
     CDS             complement(11090..12592)
                     /locus_tag="PLG42_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU29890.1"
                     /translation="MVKRKPPLSFWQIWNMSFGFLGIQFGFALQNANVSRIFETLGAK
                     IEEIPILWIAAPVTGLIVQPIIGHMSDNTWNRLGRRRPYFLTGAILASLALLIMPNSP
                     ALWVAAGMLWVMDASINISMEPFRAFVGDMLPPEQRTKGFATQSFFIGTGAVVASLLP
                     YILTNWLNVRNTAQPGEIPPSVKLAFYIGAAVFLSAILWTVIKTREYSPEELKEFSHT
                     ESKTPDEKREENTALNKEIYIRRGIYWTSGGILLSIIIMKLNLEAELYILTVGAAVFG
                     LIQLISGLFVSSGKTKNGLVIVITDLFKMPRTMKQLAVVQFFSWFALFSMWIYTTSAV
                     THHIYGTSDTSSELYNRGANWVGVMFAVYNGFAALMAFLLPVVARYTNRKFTHMISLI
                     AGGVGLISIILFKKPELLIISMLGVGLAWASILAMPYAILTGSLPPEKMGTYMGIFNF
                     FIVIPQIMAASILGFLVKHLFGGESIFAIVTGGVSMFLAAAFVLFVDDKD"
     gene            12886..14202
                     /gene="fucP"
                     /locus_tag="PLG42_01375"
     CDS             12886..14202
                     /gene="fucP"
                     /locus_tag="PLG42_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOU29891.1"
                     /translation="MMDSSKEKGKLVDPQNLIPFILITSLFFLWGLANNMTDTLLAAF
                     KRIMSMTDSRTALIQVACYGLGYFIFALPAAIFIKKFSYKSGVLLGLGLYIVGCLMFY
                     PAKITGNYFHFLGALWILFGGLSILETSANPYIIAMGDEATGTRRLNLSQSFNPLGSI
                     AGVVISQMFILSGLNRATASERAVMPEETLKAIQNGELNAVTMTYVTVGFLLLVLWII
                     ILVVKMPRASDADTTVNLGQTFKRLLRNKNYVTGVIAQFFYVGAQIGVWSFTIRYAMA
                     TLNLETVNLPGGKTPEQAAATYYIASLVLFGASRFIFTGLMKFFKPSSLLLFAAVMAM
                     LCTLIVIFAGGMTGVIALVMISGFMSLMFPTIFGQAVKGLGDDTKMGGSGLIMAILGG
                     AVITFLQGKLSDITGSINYSYFIPFICFIIIAYYAASGRKNEYSLQ"
     gene            14199..15290
                     /locus_tag="PLG42_01380"
     CDS             14199..15290
                     /locus_tag="PLG42_01380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU29892.1"
                     /translation="MKILVISDLSGGYSITLPERHLYKGLAEKGAELTIISHWPTPET
                     HEMENSGLKVIYLPIIRKIDLRVVKELRRIIIQGGYDILYITYGKALTNCLLAVRNLK
                     VKIVGYIGSLNIHWHDPTAYLSFLNRRIDRMVCLSNAVKEHFVRQRGESFRDKCVVIY
                     KGYNPEWINVTRKLVRADLSIPDDSLIVCCIANVRRIKGIPYLIKSADYLPLNLPVYF
                     VLIGKGMDAPGLMKLVRKSSYRDNFRVFGFIRDVFPYTDLCDLYIQPSITEGLGRSII
                     EAMCLRKPVIVTDNGGASDLVDNGVNGFIVPVKSPAAIAEKILWCFENRDKLKGMGQK
                     SLDKVRDILDPNNTVELTYSLFKEVLAEK"
     gene            complement(15319..15879)
                     /locus_tag="PLG42_01385"
     CDS             complement(15319..15879)
                     /locus_tag="PLG42_01385"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOU29893.1"
                     /translation="MRIKILIILIMTATTLYSQKKLNADPKNTKLEWLGEKVLGKHPG
                     TIMLQSGWLIWENNRITSGEFIIDMRTIKSDEGLTRLEEHLRSDDFFSVEKFPVSKLT
                     ITGSDDFGKGTATVRGTLTIKGITNPVEFTATRQEKEDGTWFYANITIDRTQYNVRYG
                     SGKFFDNLGDKIIYDNFKVSVNLLVK"
     gene            complement(16094..16873)
                     /locus_tag="PLG42_01390"
     CDS             complement(16094..16873)
                     /locus_tag="PLG42_01390"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOU29894.1"
                     /translation="MNGKPVAVITGASRGIGRAITLSIAAEKYDIVAIARSLDSEGME
                     TLGVEVEKLGSDFFPIGLDISCTSCHVEVVRNILDRYGRIDLLVNNAGVAPLQRNDLL
                     ELTEESYDRVMNINLKGPLFFTQKIAKEMIWLKSKIQDYKPKIIFITSISATIASTNR
                     GEYCISKAGLSMVAQLFAERLTGEGILVYEVRPGIIETNMTARVKEKYDKIIEEGTVR
                     QKRWGLPADVARAVASLARGDWDFSSGAIFEVSGGLNIRSI"
     gene            complement(16940..>18150)
                     /locus_tag="PLG42_01395"
     CDS             complement(16940..>18150)
                     /locus_tag="PLG42_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU29895.1"
                     /translation="SSYQQVIPRYFSTDKNGNDPKEFLNEFFPDLRTLTRAIFLKGYQ
                     WPFSPDRINNYGSSLIDLLIYRERTEKKRRVFLDYTRNPIGPAGEKFSKNILDNEVYS
                     YLEKSNALKETPYKRLQAMNQPAIQLFLDHNIDISREPLEIAICVQHNNGGLKGNIWW
                     ESDLLHLFPVGEVNGSHGVYRPGGSALNSGQVGSYRAAKWISKKYNYNPPPFFEFLEL
                     ASAQIKEKLALVTKWISEQKRGVSFEELFKEIRVRMSETAGIIRNPGKTGKAVKEAYE
                     MLENIDHQIYASRVKEIADAFRLREHCLTHYIYLSALNKYLEAGGRSRGSFIVTDNKG
                     ILPHSKLENHWRFNICNYDRDIENKILEIKYLNKTVRTRLVEVRPIPQQELWFEKVWK
                     KNLEDKMTEC"
BASE COUNT         5212 a         3426 c         3636 g         5876 t
ORIGIN      
        1 tctcattctt ggagcagaac cagatatttc agttgcagtt aaatgccggc ttaaagtatt
       61 tattaatccc aatgtgccac ttatttactc agacacggta acaattaatg taaaaccttt
      121 tgagaaagta attgagtatc cgaaacttta tgttccaggt agttaccagg gatgggtcct
      181 gaatcagtca accccagtta ttacctctgc aaaaagtgat ggtaaatatg aaggttatct
      241 ctattttcct gatgctaata cggaattcaa actgacaaag gtacctgcat gggaagagcc
      301 caatacaata ggtgatcccg atccgagtgg aatgtcgggt actttaaaaa tcggaggctg
      361 gggtggtaac aatataaagg ttacaggtgg tccgggacaa ttcagaataa atgccgactt
      421 aaatgaggca acctattcat gggttaaaac tacatgggct ctgattggtg atgcaacacc
      481 gggtggatgg agcaccgata ccccgatgac atacaacatc aatgaaaaca cctggacagt
      541 aactgtcaac ctgattggtg gtaaatcatt caagttcaga gctaacggag aatgggcaat
      601 caatcttggt gatactggag gagacaggaa actggaatac aacggcgata acatcccggt
      661 tgctgaaaat ggtaattaca caataactct tgatcttagc aagacaatct ataaatataa
      721 gattgtcaag aactaaatga ttccagtgtg ttataaagag gttatcatca ggaggtgata
      781 acctcttttt tcacttgtaa aagttttata ttcaaagact aaaaaaatta caatgttaaa
      841 caggtttacc tattcagtga ttctctttgt cattcttatc gtattaacat gctgtaaacg
      901 tcagggcgac agtttgcagc agaagaggat ttattatctg ccagataaat ttgtcatggg
      961 tgccgacctt tcttacgtaa accagatact tgaacatggt ggggtttaca gagattcggg
     1021 gacaattaag gacccttatc ttattttcag gcaatatgga acaaatgtag ttcgcttcag
     1081 attatttcat aatccagtat ggacaagaga ggtttattct cctccctccg aaaagatgta
     1141 caacgatttt gcagacgtta aagatggaat ccgcagagct aaattacttg gaatgaaagt
     1201 atgcctcgat ttccattact ccgacacatg ggctgatcca tcaaaacaga taaaaccagc
     1261 agcctggcag tcgctcgata ttgccgcact tcgtgatagt gtttataatt atacttataa
     1321 aacgcttaaa gaactggaca aagaaggtct tatgcctgaa tatgttcagc ccggaaatga
     1381 gattaacccg ggtttcttgc ttccggaagg aagccgctgg aatggcaacg agaataacat
     1441 gatttatctt ctaaatggtg caataagtgc aatccggagt gctggcaatt caagtcagat
     1501 aaaaccccga ataataatcc atgtagcaca acctgaaaat gctgatcctt ggtttgaagg
     1561 cctctggcaa aaaggattaa cagattatga ttttatcggg ttgtcttatt actacatatg
     1621 gtcaaaggtt aaacttgaag agcttagcaa ttatatctcg atgctcaaaa caaaatatac
     1681 gaaggatgta atgataatgg aaacagctta tccatggact atagagaatg cagataatta
     1741 tcccaatata attgatacag gtaaattgac tgaccgattc cctgctaccc aggaaggtca
     1801 gttcaattat ctgaaaacac ttgttcagga aattattgaa ggaggcggcg aaggtgtttt
     1861 ctactgggaa cctgcatgga ttacatctga aatgaaggat ctctggggta cgggttcatc
     1921 atgggattgc aatacttttt ttgattttca gggtaataca atcaaaggca taaattacat
     1981 gactgaaaaa tataacatca gataaaacac actaattgta ggatgaacat tttaaaattt
     2041 tgaaatgaat aaattcctgg catcagcggt tctttttctt ttgtttctct taagtgtttc
     2101 ggggcaaatg atttcaacag atccagctgt tccgacactg ggcagggtaa ttaaaatata
     2161 ttatgataca aatcaggacg ctggtgaact tcataattat accggtgaca tttatgtaca
     2221 tacagggctt attctggaag gtagtccaaa ctggcagaaa gttataggca cctggggaaa
     2281 taatgcggtt cagcctaaac taaattatct tggcaattac aagtatgagt ttattatatc
     2341 tcccagtatt gaaacatttt ataatgtact gccgggagaa aaagtcaaga aaattgctct
     2401 tgttttcaga aactcagatg gaactcgtca aacaagaccc gatattttca tagatgtttt
     2461 tgaaagtggt ttgaatgtaa tattcacact tcctgaaagg ttttcagttg ttattgaaaa
     2521 aaacagcctg cttggagtaa gtgcatctgc tacgaatgcc gattcaattt cgttgtacat
     2581 taataatcag tacaggaaaa gtggcagaac cagggatctt gttactgata ccattattgc
     2641 ggaatcttac ggtgaatatt gggttaaagc agttgcatgg gacctgccgc gatttgctgc
     2701 agactctttc ttctgtcatg tcagaaaatc ggttgaagtt gaagaacttc cacccggttc
     2761 cattgatggt ataaattata tcaatgatac ttctgttatt ctggttcttc atgctccata
     2821 taaaaactat gtatttgcaa caggtgactt tacagattgg ctggcgtgca gtaagggtta
     2881 tatgaagaga acaccagaca gtgagcgtta ctgggtaagg attgatggcc ttgaaccaaa
     2941 gaaggaatac aggtttcagt attttgtgga tggggaactt tatattgccg atccttatgc
     3001 agataaaata cttgaccctt ttaatgatga tttcattact gaatcgacct atccaggact
     3061 gataaaatat cccaaaggaa ttaccaatgg tatggtttcg gtgctccaga cagcacaaaa
     3121 tccatataaa tggaaaaatg aattcttcaa tgctccccca ccagaaaagc ttgtcattta
     3181 tgaactgctt ttaagggatt ttgtcgcttc acatgatttt aagactctta ctgatacatt
     3241 aaattatctt aaaaatcttg gtataaatgc tgtcgaactt atgccggtta ccgagtttga
     3301 aggtaacctg agctggggtt acaatgtggc attttatttt gcacccgaca aatactatgg
     3361 acctaaagaa tatttcaaag cttttgttga cagctgccac ggcaaaggta ttgccgttat
     3421 aatggatctt gttcttaatc attgttttgg acagtctcct tttgttcaac tatacctgga
     3481 tcattacggc gctcaggaaa tagttatgaa gactccaaac ccctggttca attcagtttc
     3541 accaaatccg gtatataagt ggggagcaga ttttaaccat gaggctccgg ctactcagaa
     3601 acttgttgac cgtattactt catactggct tacagagtat aaaatagacg gtttcagatt
     3661 tgactttacc aaagggttca caaacacacc tggcgaaggg tggacatatg acgcttcgag
     3721 gattgcaatt ctgaaaagaa ttgcaggtaa aatctgggag gtcaatcctt cggcatatgt
     3781 gattcttgaa cattttactg aaaatgctga ggaaaaagaa ctcgccgaat atgggatgct
     3841 tatatggggt aatttaaatt atcaatttgc agaagcagtt atgggctacg attcagatct
     3901 ttcaggagca actcatatag ccagaggatg gaaagtacca aatctggttt cttatatgga
     3961 aagccatgat gaggaaagaa ttatgtacaa agctcttgct tatggagctt ctgcccccga
     4021 ttataatgtc aaaaatcttc agacagcttt acggaggatg gaactggcag ctgtgttttt
     4081 gctgtcaatt cccggaccaa agatgatatg gcaattcggg gagctaggat atgacatctc
     4141 aatcgattat aacggaagaa caggtgaaaa acctctgcta tggaattact tttcgatacc
     4201 ggaaaggcga agactttaca gagtttacag tgtaatgaac aatctgaggc agacctgtga
     4261 tgctttcagt aatacttttt ttacatattc cctttcaggc aaacagaaaa gcataattct
     4321 tggttcacag aacattaaag catatattgt tggtaatttt gatatttcaa cagcaacttt
     4381 tatacctctt ttcccacaaa caggaaaatg gtatgaatat tttagtgatg attcactgac
     4441 agtaaattca ttcagtgacc ctctcacctt acgtcctgga gagtacagaa tttatacaac
     4501 tgtaagactc ccatctccta aagagtatct gggtgtacag gaaatactcg ataatgagtt
     4561 gatatctgta aatgcctatc ctaatccatc aaaagaccag ttttatttta ctgtcaatac
     4621 caaactacct ggtttattga ctctgacaat ttacaattca accggaaaca caatatggca
     4681 aaagaaaatg ccggctttga atgaattcat tgttgaatgg gatggtaaag acagcagggg
     4741 gaacgacatt ccagccggta tatattttgt tcatgttcga aacggaaagt attataatgt
     4801 gataaaaatc gtaaaactga attaagtacc aaccctttta agtactattg ctgaaagtgg
     4861 ttttatgttg ataatcaaat ttttattatt ggttttgtaa tcttttttat tgtcagggaa
     4921 aagttcaata taaagagcct ttttccgtac cggtaacatc actgtttgtt cactccctga
     4981 tctgttgaag atggtgatga tttcttcaga acctttttca cgggaatagg ccagtatcat
     5041 tttattgtca tcagcaataa gaaaatcgag gtctccataa acaagaacgg gattttcttt
     5101 tcttagttta atcatctttt tgtaaaattc atttaggtta tggtcgggtt ttacagtatc
     5161 ggcaggtcgt attttatcgg ggtagaagtg agttctttcc acctcataaa caatgtcatt
     5221 ccacaccatt ggtttgcggc aatcagggtc gtcggcaccc cacattccaa cttcctcacc
     5281 ataccatata tgtggtgctc ctgtaaaagt gaactggtga acaaggaaca ttttttgctc
     5341 aagcaatgtt atactgtcag gtttattgat tttgtaatca gggttttcag aaggttttgc
     5401 acggtatttg tccagggttt tgttatagaa agaagttgaa agtcgtggag aatcatgtgt
     5461 gccggcaaca ttcatcattg ccatctggtt ttcggccgga attccgacat taagtttttt
     5521 tatccattct acaaattcgg atggagtcaa gacaggttct gcctgtgcga agaatcctct
     5581 tgccaccctg aaccatctat aattcattat tgcatcgaat tgttcgccca taaacggggc
     5641 ggggtcaagg agctggtcgg gccatttgag ccaccatatc tcacctacca gatatgcctc
     5701 aggattaatt tccctgacca cttttcggta ttcacgccag aagcccattg gtatttctgc
     5761 tgcaacatca agacggaatc cgtcaacacc gtctgaggga tcaccattgc catcggggtc
     5821 aagccatctg cgggtgacat caaatatatg tttacggagg gaagaagaat ggagattccc
     5881 ttcaaaaggc atgatggtat catctggggg aattatatct tttctgaaaa caggcatata
     5941 aatgtttctt ccgccccatc cttcatatct gaattcattt tcaggagttg cagggttatc
     6001 ataacttata atgttgtacc agtcggcata ttttgaaaat ggaccttttt ccctgatatc
     6061 tttaagtgcc cagaattcct ttcctgtgtg gttccacgag taatcaatta ttactttgat
     6121 gcctcttttg tggaattcct caacaacttt aagaaaaagt ctgtcggctg aggtccattt
     6181 ccatgttgcc gggtcatcag gggtttctga ttccatcaat tgagagtccc cttcaggatc
     6241 gggaccgaaa ttgcggtcga tatgagtata agtgcgggca tcgtatttgt gcagggaagg
     6301 tgagtcattt agtggattaa aatagatggc agtaatcccc agggaatcaa gataatctat
     6361 cttatcaagc acaccctgaa gatctccccc gtaacggcgg gcctggattt ttgcatgaaa
     6421 attaccgccc ttgattttgt cgagccaggg ctcatgtgca taccaatcgt gtccccatgg
     6481 agtgatgctc caatcctcgg gataattatc gggatatgca ccggtaatgt cctttacaga
     6541 aggatcgttg gatggatctc cgtttctgaa acgctcaaca aaaatctgat accagacagc
     6601 tttttttgac cattccggaa cagtataagt tgttttgtag ttgcatccgc aagccaggag
     6661 taaaatggcg attattttat aaaaattctg aatccccatg gctcaattat aagcggttta
     6721 tcgtcaggca tttcgattac tgaatggttg aaataatcgg taaaagtacc ccggatgttt
     6781 ttaagaaccg gttttacggc cacctcttta tctctaagat tcagaaaaac tatcaaaagg
     6841 ttaccatctt tttctctgct aaaggcaaat accttgccgg gctgattggt tttaataatt
     6901 tccatcttgc ctccttcctc tccattccag agggcctgat tttctttttt aaggcgtata
     6961 agttttttat aaaaagctgt cattgtacat gtatcccatc tgatagtatc ctttgtaaaa
     7021 aagcgaagac ttttattaag gcacacttcc tggccattgt aaagcaaagg tacacccctt
     7081 gtggtaaaca tgaagacagc aaaaggatac tgggcttcac ccattcgtga tgttatggtt
     7141 ccgttccatg aattttcatc atgattgtct aaaaatacca tccggtaagt attctgcgga
     7201 taaacgaaaa gctctttttc gtaatatttt tcaagtgaat ttacgctgtc ttttttgcgg
     7261 gctatctggt tcatcagatg atgcaattcc caggaatagt tcatgtcaaa gccctttttc
     7321 ataaattcat tctggccctc attttctgcc agcagcagaa caggtttaat tttccatagt
     7381 tctgccctta ccgtttccca gaattcagga ggtgttttat gcggatggtc aaccctgaaa
     7441 ccgtcaaccc caagttcaac ccagaattta agcgcattca gcatatattc atgaaggcct
     7501 ttattagtcc aatcgaattg tatgacatca gtccagtctg ttcccagagg tggaataaaa
     7561 ttaccgtcct tgtcttttac gtagaactct ggatgttcag ttgttaaagg attatcccat
     7621 gatgaatggt ttggaaccca gtccattatt acatgaaacc ctgcttcgtg agctttgtca
     7681 agtaaaattt tgaaatcttc cagagtgcca aattctggat ttactttcat ataatccctt
     7741 actgaataat agcttccaag ttcatccttt cggtttttta ttcctatagg gtggattggc
     7801 ataagccaca ggatatcaac tcccagttcc ttaagtctgg gcaggtgctg ggtaaatgct
     7861 ttgaaggtcc cttcaggtgt aaattgcctt atatttactt cgtaaagaac agcatctttt
     7921 gaccattcgg gatgaacaac ttctgaaaca aaaggctggt aattgttacg gtttctttta
     7981 tgcaaacaac ctcctgccgc aagagcggct acaaagaaaa ttgacagaac atagagaata
     8041 attttttcca ttattttttt attaaactat taaactcatt tatatgaaat aaaaataatt
     8101 atcctgtttt gattttaatt gaatcttttg gagaaatatc gaagtttttt tctttaactg
     8161 atatcggaag aattctctca gaaaggtttc tgacaatgca aaaagatttt gttactttaa
     8221 tctcgaagag tatttcttta aacctggcat gaaatgaata tgatttccat ttatccggaa
     8281 tcagcgggtt aaaatttatc ctgtcccctg caatcctcat tcctccaaag ccttcagcga
     8341 tagcaagcca gtttccagcc atgcttgtaa tatgcaaacc ttcagccact tctttattat
     8401 aatcgtcgag atcgagtcga gtgcttctta tgaaaagctc atatgcttta tctatatttc
     8461 ctatccgggc tgcaaggata gaatggactc cggccgaaag tgaggattca tgaacagtca
     8521 ttggttcata aaattcaaaa ttccgtttaa tagtctctgt atcatattgg tcttcaaaga
     8581 aatataaacc ctgtaaaaca tccgcctgtt tgatatagca cgacctcaga attctgtccc
     8641 atgaccagtg ctggttaata ggcctgtttg aaacaggaat tgctgaagct ggtttgagtt
     8701 ccttgtcgag gaatccatct tgctgtaaaa aaatttttct ttttggatcg tatgggaagt
     8761 gcataccatc gataattttt ttccattttc cggtttcaga tttatatctg aattttgttt
     8821 tcaggaaaat tttctcaagg gctgccggat tgcttttttt aagttcttca atattatgaa
     8881 tggtatagga aagggtccag acagcaatag aattggtgta ccaattatta tttacattgt
     8941 tttcatattc atttgggcct gtaactccca gtataacata cttcctcagg ttttttgaaa
     9001 aagatactct ctggctccag aatcttgaga taccgataag aacttcaagg ccataatctt
     9061 tcatatagtc tttgtctccg gtatgtctta tataattgaa gatggcatag gctattgcac
     9121 catttctatg gatttcctca aaggttattt cccattcgtt atgacactct tccccattca
     9181 ttgtaaccat tgggtataga gctgctcctc ctctgaatcc aagttttctt gcgttttcta
     9241 tacttttctt caaatgcctg tatcggtaaa gaagaaggtt ctttgataca tatgctggag
     9301 cagttttaag aaagaaagga agaacaaaag cttctgtatc ccagtaagtg cttcctccgt
     9361 atttttcccc ggtaaaaccc ttgggtccta tattcagccg ttcatctttc ccagtatatg
     9421 tctggttaag atggaagata ctgaacctta tgccttgttg tgctgcagga tcaccatcaa
     9481 tttttacatc ggagatactc cagatatttt cccactcttt tttatgagca gagaaaagat
     9541 tttcaaatcc tttcttttta gcatagttga gacgcgaggt tgtaatttta aaatgatctt
     9601 ttaccgggtg gtacaaggaa gattgcaccg atacgtattt gacaatgact atttcgtcgc
     9661 cgggttgaaa atccagatga ataatatttg cagcatatcc ttttcggttt ataacttctg
     9721 gaacaagatt taatttattt ccgtttaatg taagttcata tgtcataccg actgtcacgt
     9781 aaaaatcggt tctcagggtt ctggcagtaa gcattcccaa accgggttca gcaaggttgt
     9841 caatgtcttc ccagaatttt tctccgtagt tggtgttttc atttttaaca tcagcatcga
     9901 gataaatatt cagtgagaga gtgccgctaa gattcacggg attgatttta tatttaatta
     9961 tgcacaggtc agggtctgcc atactaagaa atctaaggct ctgaatccta atttcttttc
    10021 tgctgaaagg tcttacagtg aaatttcttt ccagaaaacc ttctttcatg ttaagaacac
    10081 ggttaaattt taatggctta tgttttgcca gatctatttc ggctccattc acatttacat
    10141 caattcctat atattttggt gagtttggaa tcttggcata atattcagga tatcctactt
    10201 tccaccatcc cactttggtc ctgtcgggat aatatattcc tgcaacatag cttccctgaa
    10261 gtgaatcacc agagtatttt tcctcgaaat ttgccctgtg gcccatatat ccattgccta
    10321 ttgagaaaat actttcggct tccctgttta tttcagggtg aaatccttcc tcttcaattg
    10381 tccattcatg gattttgaaa agattggttt ttatcattga tttatagttt ttaaggcggt
    10441 taattttaaa aagctcattt cagataggtc atttataaca tagtcagctt caataagtat
    10501 ttccttattg ccaactccta taccatacat tccggctttg tgggctgctt ttattcctgc
    10561 taccgaatct tcgaaaacta tgcatttttc aggtttaaca ttcagtattg atgcagttac
    10621 aagaaaaatt tcagggtcgg gtttggcgct tttaaccata ttaccgtcgg ttattgtatc
    10681 gaacaggtct ttcattctaa ctttttcaag tattaaaagt gtatttttac tggccgagcc
    10741 tatggcagtt ttaattccat ggctctttag atctgtcaac agttcgatag ctccgggcag
    10801 aatttcggaa ggtgtcatac gggaaacaaa ttcaacatac cacgtgtttt tcatttctgc
    10861 aaactttgct ttctctgaat cggtgtattt tttacctgag agttcaagaa ttatttcgag
    10921 agatgcaaga cggcttactc ccttaagttt ttcattttct ttttcactga aaggaattcc
    10981 cagcatatcg gcgagtcttt tccatgcaag aaaatggtat ttagccgtgt cgacaattac
    11041 tccatcgagg tcgaatatac aggcatcgaa ccacatagtc tgtattcagt taatccttat
    11101 cgtcaacaaa tagcacaaat gctgctgcca gaaacattga tacaccgccg gtaacaattg
    11161 caaatatgct ttcaccgcca aataggtgtt ttacaagaaa accaagaatt gatgctgcca
    11221 ttatctgagg aattactatg aagaaattga aaattcccat atatgttccc attttctcag
    11281 gaggcagtga accggtaagt attgcatagg gcattgcgag tatgcttgcc catgcgagcc
    11341 ctactccaag cattgaaata ataagtaatt cgggtttttt gaacaggatt atcgaaataa
    11401 gtcccactcc tcccgcaatg aggcttatca tatgggtaaa ttttctgttt gtgtaccgtg
    11461 caacgacggg gagaagaaaa gccattaatg cagcaaagcc gttgtaaacg gcaaacatta
    11521 cacctaccca gttggctcct ctgttataga gttcacttga agtatcgctt gtgccgtata
    11581 tatggtgggt aacagctgaa gttgtgtaaa tccacatgga gaatagtgca aaccaggaga
    11641 aaaattgtac cactgccagc tgtttcattg tccggggcat tttgaacagg tcggttatga
    11701 caataaccaa cccgtttttt gttttgccac ttgaaacaaa tagaccggaa atcagttgta
    11761 tcagtccaaa aacagcagcg ccaacagtaa gtatataaag ctcagcttcc agattcagct
    11821 tcattattat aatgctcagc aatatacctc cagaagtcca gtaaataccg cgcctgatat
    11881 atatttcttt attaagcgct gtgttttctt cccgcttttc atccggtgtt ttgctttctg
    11941 tatgtgaaaa ctctttgagt tcttcagggg agtattccct ggtttttatt actgtccaga
    12001 gtattgctga taaaaagaca gccgcgccga tatagaatgc aagttttacc gatggaggta
    12061 tctcacctgg ttgtgcagta tttcgtacgt taagccagtt tgtaagaata tagggtaata
    12121 gtgaggctac gacggcacca gttcctataa aaaaactttg cgtggcgaag ccttttgttc
    12181 tttgttctgg agggagcata tcgccaacaa aagctctaaa aggttccatt gagatattta
    12241 ttgaggcatc catcacccat aacatcccgg ctgccaccca gagagcaggt gagtttggca
    12301 ttatcagaag agcaagggaa gcgagtattg cacctgtgag aaaatatggc cgtctgcgcc
    12361 ccagtctgtt ccatgtatta tcactcatgt gtcctataat aggctgtact atcaggccag
    12421 taacaggagc agcaatccag aggataggaa tctcttctat ttttgctccc agggtttcga
    12481 aaatgcggct gacatttgca ttctgaagtg caaatccgaa ctggataccc agaaatccaa
    12541 aactcatatt ccagatttgc cagaagctca gaggtggttt tctttttacc atgtttagtt
    12601 aatattcgtt taaataatca ggcagggttc tgctcaaaaa tatgtaaaat catctaataa
    12661 aaatcttgta tatgtcattt attttcaatg aaatcagtaa ttaaaacggt atttatgact
    12721 gtcagtattt atttcaaacg ttttcggata aaatggattt ttaagaaaaa atttctgtga
    12781 actttccgat ttttaaaaag aattcaattt ttttattggt tgattggtat atgctgttat
    12841 ttttgacact ttattttaaa tgttgtttaa tttcataaaa ctgacatgat ggattcatca
    12901 aaagagaagg ggaagcttgt cgatccccag aatcttatac cattcattct aataacttcc
    12961 ctgttctttt tgtgggggct ggctaataat atgactgata cacttcttgc cgcttttaaa
    13021 aggatcatga gcatgaccga ctcaagaact gcacttattc aggtggcatg ttatggttta
    13081 gggtatttta tttttgcatt gccagcagcg atctttataa agaaattttc ctataaatct
    13141 ggtgttttac ttggactggg gctttacata gtggggtgtt taatgtttta tcctgcaaaa
    13201 attacgggta attattttca ttttcttggg gcattatgga ttcttttcgg gggactttca
    13261 attcttgaaa catctgccaa tccttacata attgcgatgg gtgatgaagc aactggaaca
    13321 cgaaggctta atctttcaca gtcatttaat cctttaggtt ccattgcggg agttgttata
    13381 agccagatgt ttattctttc tggcttgaat cgtgcaacag cttcagaaag ggctgtaatg
    13441 ccggaagaga cactaaaggc cattcaaaac ggagaactga atgccgttac aatgacatat
    13501 gttacagttg gttttctttt acttgtatta tggattataa ttctggtagt taagatgccc
    13561 cgagcttctg atgctgatac aacagtcaat ctggggcaga cgttcaagag gcttttacgt
    13621 aataaaaatt atgtaacagg agttattgcc caattcttct atgtaggcgc acaaattggt
    13681 gtatggtcgt ttacaatacg ttatgccatg gctactctta accttgaaac tgttaatcta
    13741 ccaggtggga aaaccccaga acaggcagca gcgacatatt atatagcttc acttgtactg
    13801 tttggagctt ccagatttat tttcacaggg cttatgaaat tttttaaacc ttcgtcgctt
    13861 ttactgtttg cagcggtgat ggctatgcta tgtacattaa tagtaatttt tgcaggtgga
    13921 atgacaggag taattgccct ggtaatgatt tcagggttta tgtcgcttat gtttccgaca
    13981 atctttggcc aggcagtaaa gggtctggga gatgatacaa aaatgggagg ttcaggtctg
    14041 ataatggcaa tacttggtgg agcagtgata acatttctgc agggtaaatt gtcagatatt
    14101 acaggtagta taaattattc atatttcata ccctttatat gctttattat catagcatat
    14161 tacgcagctt cgggcaggaa aaatgagtat tcgttgcaat gaagattctg gttatatccg
    14221 atttaagtgg tggttattca attacacttc ctgaaaggca cctttataaa gggctagctg
    14281 aaaagggtgc tgaactaacc atcatatcgc attggcctac ccctgaaaca catgaaatgg
    14341 aaaattcagg actaaaggtt atttatcttc ctattataag aaagattgat ctgagggttg
    14401 taaaagaact aagaagaata ataattcagg gtgggtatga tattctgtat ataacttatg
    14461 gcaaagctct cacaaattgt ttgctggcag tgcgtaacct gaaggttaaa attgttggat
    14521 acatcgggtc gttgaatatt cattggcatg atcctacagc atatctctca tttcttaaca
    14581 ggcggattga caggatggta tgcctgagca atgctgttaa ggagcatttt gtgaggcaga
    14641 ggggggaaag tttcagggat aaatgtgtgg tgatttataa aggttataat cctgaatgga
    14701 taaatgtaac aaggaaattg gtcagggcag atctgtccat tcccgatgat agccttattg
    14761 tatgttgtat tgcgaatgta aggaggataa aagggattcc gtatcttatt aagtcggcag
    14821 attatcttcc cttaaatctg ccggtttact ttgtattgat aggtaaaggg atggacgccc
    14881 cggggcttat gaaacttgtt agaaagtcat catacagaga taatttcagg gtgtttggtt
    14941 ttatcaggga tgttttccct tataccgacc tgtgtgatct ttacatacag ccatccatta
    15001 ctgaagggct gggccgttca atcattgaag ccatgtgcct cagaaagccg gttatagtta
    15061 ccgacaacgg cggtgcaagt gaccttgtcg ataatggagt aaatggcttt atagtaccgg
    15121 ttaaatcgcc tgccgcaata gcagagaaaa ttctatggtg ttttgaaaac agagataaac
    15181 tgaagggaat gggtcagaaa tccctggata aagtcaggga tattcttgac cctaataata
    15241 ccgttgagct aacttatagc ctttttaagg aagtattggc tgagaaataa ttataaatga
    15301 aattatcatt tcagagagtc attttacaag aaggttgaca ctcactttga agttgtcata
    15361 tatgattttg tcccccagat tgtcgaaaaa ttttcctgac ccgtacctga cattatattg
    15421 agtgcggtcg atagtaatgt ttgcataaaa ccatgtgcca tcttcctttt cctggcgtgt
    15481 tgcagtgaat tcgactggat ttgttattcc tttaattgta agggttcccc gcactgttgc
    15541 tgttcctttg ccgaaatcat cgcttccagt aatagtaagt tttgacacag gaaatttttc
    15601 aactgagaag aaatcatctg atctaagatg ttcctcgagt cgtgtcaggc cttcatcgct
    15661 tttaattgtt ctcatatcaa taataaattc cccggatgta atccggttat tttcccatat
    15721 gagccaacct gattgcagca ttatagtgcc cgggtgttta cctagaactt tttccccaag
    15781 ccattcaagt tttgtatttt ttggatcagc atttaatttt ttctgactgt ataatgttgt
    15841 agctgtcata ataagtatta ttaaaatttt aattctcatt tatatattga ttaacatttt
    15901 ggaatataaa cagaggaaca gggtaattgt tcaattgaat ctttatcagc aattaatact
    15961 cagggatttt aactttttaa ttcgaatttt gcaggagttg acctgaagga ttaagccagt
    16021 ctcatatatc gggttgatta agaattccag gttagaaggt tcaaccttga agtaatgata
    16081 ttggtcttga ttattaaata cttctaatat taaggcctcc gcttacttcg aatatggcac
    16141 ctgatgaaaa atcccagtcg ccgcgggcta gggaagctac agccctggca acatcagcgg
    16201 gcaaccccca tcgtttttga cgtacggttc cttcttcaat tattttgtca tatttttctt
    16261 ttactctggc ggtcatattg gtttcaatta ttccgggtct gacttcgtac acaagtatgc
    16321 cttcaccggt gagtctttct gcaaacagct gtgccaccat acttaaacct gcttttgaaa
    16381 tgcaatattc tcccctgtta gttgaagcta tagttgctga aatagatgtt atgaaaatta
    16441 tttttggttt ataatcctgg atttttgatt tgagccagat catttccttt gctattttct
    16501 gggtaaagaa gaggggtcct ttaagattaa tattcataac cctgtcgtag ctttcttctg
    16561 taagttcaag caaatcattt ctttgcaaag gagcaacccc ggcattattc accagcaggt
    16621 cgattcttcc atacctgtca agtatattcc tcacaacttc cacatggcaa ctggtgcatg
    16681 agatatcaag tcctatgggg aaaaagtcgc ttccgagttt ttcgacttca acgccaagtg
    16741 tctccatccc ttcactgtcg agggatctgg ctattgcaac tatgtcgtat ttttctgcag
    16801 caatagaaag tgttattgct cttcctatac ctctactggc accggtaatt acagcaacag
    16861 gtttgccatt cataggcttt tggattagat caggtttatc aggttttgac tggtaaaggt
    16921 aataatttga tgcatcattt caacattctg tcattttatc ttcaagattt ttcttccaca
    16981 ccttctcaaa ccataattcc tgttgaggta tgggacggac ttctaccagt cttgttctga
    17041 cggttttatt cagatattta atttcaagaa ttttattttc gatgtcacgg tcgtagttac
    17101 aaatattgaa gcgccagtgg ttttcaagtt tactatgggg aaggattcct ttattatcag
    17161 taacaataaa cgatcctcgg cttcttccac cagcttccag atatttattc agtgctgaaa
    17221 gataaatata gtgagtcagg caatgctctc tcagcctgaa tgcatctgca atttccttga
    17281 ccctgctggc atatatctgg tggtctatat tttcaagcat ttcgtaagcc tctttaactg
    17341 ctttacctgt ttttccagga tttcggatta tacctgcagt ttctgacatt cggactctta
    17401 tttctttgaa aagttcttca aaagatactc cccgtttttg ttcagagatc cattttgtca
    17461 caagtgctag tttctctttg atctgcgcag aggccaattc aaggaattcg aaaaatggag
    17521 gaggattata gttatatttt ttcgatatcc attttgctgc tcgataactg cctacctgac
    17581 ccgaatttaa tgctgaacct cctggtcggt atactccatg ggaaccattc acttcaccta
    17641 caggaaatag gtggagaaga tcagattccc accagatatt tcctttaaga ccgccattat
    17701 tatgctggac acaaattgca atttccagag gttccctgct tatgtcaata ttatgatcga
    17761 gaaaaagttg aattgcaggc tggttcattg cctgaagtct tttataaggt gtttctttaa
    17821 gagcattcga tttctcgagg taactataaa cttcgttgtc aagaatattt tttgagaact
    17881 tttcacctgc tggacctata gggtttctgg tatagtcgag aaacaccctg cgtttttttt
    17941 ctgttctttc cctgtaaatc aggaggtcga taagtgatga accgtaatta ttaatccggt
    18001 cgggtgagaa aggccattga tatcctttaa ggaaaatagc acgggtgaga gttctcagat
    18061 caggaaagaa ttcattcaga aattcttttg ggtcatttcc gtttttatcg gtcgaaaaat
    18121 atcgtggaat tacctgctga tagctgcttg
//
LOCUS       DAOSNB010000014        18127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3102_length_18127_cov_4.901726, whole genome shotgun sequence.
ACCESSION   DAOSNB010000014 DAOSNB010000000
VERSION     DAOSNB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_18127_cov_4.901726"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..761
                     /gene="pepE"
                     /locus_tag="PLG42_01400"
     CDS             45..761
                     /gene="pepE"
                     /locus_tag="PLG42_01400"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOU29896.1"
                     /translation="MKLLLISNSTNAGEGYLEHAFPHIKSFLGSKQAEILFIPYAAVT
                     FSYDEYEKKVSQKFIEGGYQIKSIHHFTDPVAAIKKAEVIAIGGGNTWKLLKMIRENK
                     LIEPVREKVFSGTPYIGWSAGANVACPTIRTTNDMPITEPDSFEAFCLIPFQINPHYL
                     DVNPEGFAGETREQRIREFIELNPGIYVAGLREGTMLLIEGKNVKLIGPKSARIFIKN
                     AEPFEAGNNDDLSFLLQKNI"
     gene            811..3417
                     /locus_tag="PLG42_01405"
     CDS             811..3417
                     /locus_tag="PLG42_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOU29897.1"
                     /translation="MKLVVKIFAWIVAGIIITLTAMIVTAFVFQNQAVVAVIDSINRN
                     LSTKFEFEKYRLSLIKRFPKASFELLNLTVFSSESFDRTQWTGINTDTLLRAGTASLE
                     FKITDILKKKYSIESITISDGELFLITDSIGGINFKISDSNKGNYDNEIDLDKIVIRN
                     LKATYLNKATDISISGVINNGKLRSRIGGKNLDFSCTSGILVNQFNIGSLNIKPDIQV
                     YADINLRKSDEQLTFQNSILKFEGLSLRLTGYIKPENELLLKIEGQNIDLSKAKKYLP
                     QKLIEKYTDFTAGGLLNASCILSGPLNRTKNLFTQMAFSIDKGKLYNRKTDILVENIS
                     FSGNFTGGDGSDYPRGKLNIQPCSFMIGNAEYYISFSAENLKNPFIKLNFSGEIVPED
                     LKKLVSIPWLISGEGSARLNLKLEGPLPKKTKKLNAADIFNLNPQANIQFKSLSINHK
                     NERFSFNDASGNIFIASSLWTDDLVFSYNNQRIKITGEFGNLPAWLAGKPEIIKINAS
                     ITADNIDVINFSASNSTDKSNIKRALKMPEGIEADISFVLNNFKWRKFTAANISGTLN
                     YKPGLLNFTNFSATTLDGLVSGIFVLSRQKTGLPFASQGNFFIENIDINKCFSSFDNF
                     GQDFIHSEHLSGNLSGTLKIMMQHDSLLKPDIRSVNAEGNYLIENGSLTNFEPVKALS
                     KFININELENISFSRLENNFYIKNNYLAIPQMEINSSAVNFTVSGRHNFDNTYEYHVK
                     VYLSELLSGKMKKNQTITDFGAVEDDGLGRTSVFLKITGQGNNIKVSPDLKATGVRIR
                     ESLNNEKSNLIKILNEEHGLFKKDTAMKTEPAPRPKFNIEWEETNNVEIKKDTSGNQE
                     KNNILNRIFKKK"
     gene            3444..4598
                     /locus_tag="PLG42_01410"
     CDS             3444..4598
                     /locus_tag="PLG42_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU29898.1"
                     /translation="MNIYSHKTLWKLILLFLAVGIGMFSFCYTYLLVRNLKSEERKKI
                     EIWAEAIRQLNFAADTGQTLEFLSSIIEGNTTIPVILADGKDSIISARNYDPMKLTAP
                     NFLQTRLKKIKEKSEPVIIYLPDGHINKVYYEDSIILRKLIYYPIVQLSIIILFILVS
                     YLAFNSSRKAEQNQVWVGMSKETAHQLGTPVSSLTGWVEMLRSKYPEIPFTEEIATDI
                     ARLEKITERFSKIGSRPELISTNIIPVLRQTIDYLRSRASSRTVFINEYDPLAEIVVK
                     FNPPLISWVIENVCKNAIDATNGEGTINISVTEMRNYVIIDIRDNGKGIPRSAHKKIF
                     SPGYTTKQRGWGLGLSLSKRIIEEYHKGKIFVKYSEPGLGTCIRIMLRKN"
     gene            4639..5889
                     /locus_tag="PLG42_01415"
     CDS             4639..5889
                     /locus_tag="PLG42_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOU29899.1"
                     /translation="MKRHQVEISAPAGSWASLTAAVQGNADSVYFGAEQFNMRASSSE
                     NFSLSDLPEIVSFCKSHGIKCYLALNIVIYDDEINKMKKIIDTAAKSGISAIIATDQS
                     VINYAFSKGLEVHLSTQLNITNTNTLKFYSQWADVAVLARELSLEQIKNISDEIKKRK
                     ICGPSGKPFRIEIFAHGALCMAISGKCYLSLHEYNKSANRGLCNQVCRRSYIVTDKET
                     GFELEIDNQYIMSAKDLCTIGFLDRIIEAGAEILKIEGRARSPEYVRTVTSCYDEALR
                     AIEQGTYTPENIKKWTERLSTVFNRGFWDGYYLGRKMGEWTNKYGSSATMKKTYIGKI
                     TNYYNKIGVAEIKLENGDLSIGDTILIIGPTTGTLEYRVEEIRDSEHMITEKALKGTF
                     CSIKTDHYLRRSDKVYKWEERDKE"
     gene            complement(5911..8733)
                     /locus_tag="PLG42_01420"
     CDS             complement(5911..8733)
                     /locus_tag="PLG42_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU29900.1"
                     /translation="MKRILIIAEVVLLLILLINFFYYRNLYRNQISYMIELLDRQVRI
                     VGLEVDSINYAFTSDLGQITFTNDAYYFFDRTKPEIKNRITEQLKLFYSKYRDFVVKI
                     RLYDDKQNEYTLSKDETKNEWIEGEFVSLDQRPIEPKEKLIENNGEFNYYMVIFKGGK
                     ASGNIVTTVDYRKYFGKLFSKYNLKDYQWQWVLTENGEVVFDNNENLPEYTEKGKITE
                     GLKEGIVSNLIHYAIINNERVEVLSSFYSTQLLERDLGVIFSAPADFFQKYLIRNSVF
                     IVTATILLIQLIILVFWRVLKIQENKIQELHDSENTLRRLIEEMPVGIIIYNDKREIL
                     KANKVAAEYYSYENEAEMTGKIFPETTLKIDNAYFSKYLGKSFNPDQFLVIKKPFGEL
                     ILYHTSIPVVYQREKASLEVLIDVTMLESARKQEVKANIAKTEFLARMSYEIRTPLNG
                     IIGMSDILNKYDLSPEVRDIVYLLRRSTEVLLGIVNDILDFSKIESGKMILEETAFNI
                     REEVYYAIDLAKPLAKERNVTILCEVDDSVPETVIGDPYRLRQILVNLINHSTSNTEN
                     GEVHVRILKKSEKSGVITIGFEILDTGKAFDKAELKKIFGDIVNIESIVPGNYNESMF
                     STLIARQLIELMGGTLTAVSPSGISGDNGTKITFTIQVHSNERIKKDIDTSTIKSIDQ
                     IKTLVITGPSGRDEELLSIFHRAGLQISVTSYTKTTLNQILVNSTIPKIKYDLIIITD
                     DENFDGIEVAQMLWDNNLSSRYRVFMVSTNDRKGNYLKCLTFGVDYYLVKPFDVHEFQ
                     NAVSEVFHIEPVGLILHEGEEAKSLNILIVEDNKMSQKILAKMIESLGYKCDVAEEGY
                     EGYQMAFSKKYDVIFMDLFMPEMDGYESARRIFEARPEVLIVAVTADNMPETRKKAEM
                     YGIKEFITKPVRLDDLKRIILKYFIK"
     gene            complement(8780..9706)
                     /gene="rsgA"
                     /locus_tag="PLG42_01425"
     CDS             complement(8780..9706)
                     /gene="rsgA"
                     /locus_tag="PLG42_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOU29901.1"
                     /translation="METGVVVKSTGSRYKVMIPDGRNIECTIKGRFRIKDIQTTNPIA
                     VGDRVKFSLLPGKETGIISEIEERKNYIIRKASNLSKKYHIIAANIDQVFLMVTIRMP
                     KTLQEFIDRFLATSEAYRIKSIIIFNKIDLYDEEDTAKMNYLASLYSDIGYDCYCISV
                     KTGKNVELVKEAMKDKVNLIAGNSGVGKSSLLNLLNPSLKLKTDELSPYHEQGKHVTT
                     FPEMHPMPFGGYVVDTPGLRGFGIIDMDKNEIYHFFPEIFRKSKECRFHNCLHVNEPG
                     CAVIPAVESGEIDIMRYRSYLNILDDENRKYR"
     gene            complement(9910..11115)
                     /locus_tag="PLG42_01430"
     CDS             complement(9910..11115)
                     /locus_tag="PLG42_01430"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU29902.1"
                     /translation="MANIKTKRGVIGILTGGGDVPGLNPAIRAVTIRANREGFKVIGL
                     KRGWAGLTEIIRDNKADNSRCYQVLTNEIVNKAGRTGGTFLHTSRTRPSHMSKNDVPE
                     HLKSTYNADMNDLTPEVLKNLEWLGIDYLIPIGGDDTLSYAVHLYTKGVKVVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTINMTNYLRTCAGSHERILVIEVFGRYAGFTSILPTMAG
                     AANRCVIPEYKFDIEHLTKLLVDDRNENPSRYSIVLVSEGAMFKGGEMVFENMEKDAY
                     GHAKLGGIGDLVSAKIKELSPKFNKGKTINIINQKLGYLVRSGDPDAVDSIVPMAFGN
                     LALDLILRGIHGRLVVLRNGRYDNAPVDIVTSQKKFVNVEEYYNTERLRPHYHSFEMK
                     PFFLMAAEG"
     gene            11257..12195
                     /locus_tag="PLG42_01435"
     CDS             11257..12195
                     /locus_tag="PLG42_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOU29903.1"
                     /translation="MIANKKEPCNNYSVLITGGSGMVGRYLTSFLLEKGYTVSHLSRV
                     ANLFGRVRVFRWDPEKEILDPTALENTDYIIHLAGANIGEKRWTEKRREEIIRSRVDS
                     ANLLLNTVIKNRFPLKAFISASATGYYGSITSETIFTEEMGPGEDFTGIVCQKWEEAA
                     EKFAKSGIRTVKLRTAVVLEKNCAVLKKLQLTEPFGILPVPGSGKQYFPWIHINDLCR
                     IYIKAVEDSQMNGAFNACAPEQITFIDFVKKYAELTGKKKIITKVPAVLLKIAFGKMS
                     EIILQGSRVSSSKLISAGFTFDFPTMDKALKNILGS"
     gene            complement(12204..13538)
                     /locus_tag="PLG42_01440"
     CDS             complement(12204..13538)
                     /locus_tag="PLG42_01440"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU29904.1"
                     /translation="MNYINLVEKLLKKCLKLGADVAEVYFQSERNLSIQILNSEIETI
                     EEASTQGVGFRVFTGNRMGFSHCNNFSDRSVEEAILRAIEFAKLSTPDEYNVLTDDKT
                     VSEVNGLYDQNLGNIPVDNKIKMAIELEKEALKDQRITKSSGSSYRERETEIYIANSN
                     GILKNYKTSSCSLGVSVVAEKGEQKTTGGESCSRVFFEDLLPIEEIAAVAARKAFEML
                     DPVMVSTQRATVIFDADVGRSLLGGIISAINGERVIQGASFLRNSLGEKIASPLLTIV
                     DDGIKPKGLASCPFDGEGVPTIKNIIIENGILKSFIYNLKAAKRAGVKSTGNATRGGF
                     SSLPGIGVHNVYVPAGKSLKNEIIAATRRGFLVKEVTGYGIDPVTGNFSGGASGLWIE
                     NGEIVHPVRGLTIAGKADEILNAIDMVGNDLDMNRTFATPTFRVAEMQIGGR"
     gene            complement(13558..15084)
                     /locus_tag="PLG42_01445"
     CDS             complement(13558..15084)
                     /locus_tag="PLG42_01445"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOU29905.1"
                     /translation="MKRTYVSDIPRRKFLKLGIKGGIIVAASPYMIEYLTSCSGKQNM
                     FPPPEINREMLDKVIAHALKKGGDYADIYIENRITRQILMEESKFKSGLYGVSQGAGV
                     RVISGNRTGYAYTEEISYNKLLRAAEIASYVAQNGNLIIPRPVEVAENPSYITVKVPL
                     QDVADEKRLEIIQRADQAALSYDRRIKMTIIEYNDEIRSRIIATSEGIYCRNELPLLF
                     FIVQVMSDDGNSRHMARERLSYHSGFEMFDLVTPEKVAQNAAREAIAMLEAKDAPAGV
                     MDVVMQNGWGGVLVHEAVGHPLEADNIAKGVGAFTGKLGKKVASDCFTMVDDGTLPNF
                     RGTIDFDDEGTPVQRNVLIENGILKGYMTDVLSAKQLSLPRTGNGRRESFRNIPIPRM
                     TNTFIEKGKDKPEDILASTKKGLYVQSLSGGSVNPVTGVFNFTCREAYLIENGKKTYP
                     VKGATLIGSCMDVITNIDAVGDDLDFGPGICGKGQSAEVTAGQPTVRIRGINVGGTRG
                     "
     gene            complement(15149..17113)
                     /locus_tag="PLG42_01450"
     CDS             complement(15149..17113)
                     /locus_tag="PLG42_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU29906.1"
                     /translation="MKKIILLIVLLLTSRFLISQNYNNIGSQGDSATGREKKSNYLLT
                     GFRHGVSYFPEVIYRGVEYEAGETLSFDRYHSLEVIYMWLERWAEKYPDIFQLYEVGR
                     SYEGRPIYQVTISARSSGKDTEKPGAYFEGGRHSGEITASESALWLIKYLVENYGKDP
                     EITHLVDTRTIYIRPVNNPDGHNLYLKTAQSNRSTVRPFDNDGDGLLDEDSPEDLDGD
                     GLILNMRWKDEKRGTYIPDPADPTGRLMKQVQAGKGIYLVAQEGFDNDGDGRVNEDGI
                     GGLDLHRNYPENWRPQSEETGRGYTQSGAGEYPLSETETRSVVTFLLSHPNVYVVNSM
                     DTSVPMHLRAPSTSASEERMYPEDLSWYRYFDELGKKITGYSRAGDVFNDYNRGNPLF
                     GHGPDFGYWYFGAIWYGDEIWNGGRNKDYNGDGTIDQSEILKWDDEENNGDGFIEWKP
                     ARHPVYGDVELGGFNPKFFSQNPPPRHLEKWVSNQALFNLELIKALPELKWENIEIKK
                     IKSYRKDSADYQIRVSYKNTGLLPTALKQAHLVKIVRDDRVVIEIDSAAFKGDKRIFK
                     VLSPSPEQGRENRSGRDAFSDSPVLQQRSALRSISQNVPDTKGGAVSTVIFEVRVYGN
                     SYFSGKASVYSTRGGVLKNKEFYVKQSFNN"
     gene            17237..18043
                     /locus_tag="PLG42_01455"
     CDS             17237..18043
                     /locus_tag="PLG42_01455"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOU29907.1"
                     /translation="MLYITDYHIHTNYSDGTAEPEEYISTAIKKGFKEIGFSEHLNLF
                     RTDLTWCIDMQKVPDYISHISSLKNQLDNIKIKTGFEIDYYPGKEKEINDIISSLNID
                     YAIGSIHFIGENTVDSSEDFYNGKSFNDIFVHYFEIVFEAIKSGLFDFIAHCDLIRIF
                     GLKYHGNPEPLYRHLAHLMAKHEVALEINTNGKNKPLGEFYPDVRFLHLFKKENVPVC
                     VNSDAHNPGRIGEYFNEAYQLLKDAGYTEMCTFNRRERYMIPVDFSLLST"
BASE COUNT         5610 a         3683 c         3020 g         5814 t
ORIGIN      
        1 agtaagaatg attatctttg cggcaagtat ttaattccta ttcaatgaag ctgttactta
       61 taagcaattc aaccaatgca ggggagggtt atcttgaaca tgcatttccc catataaaaa
      121 gttttcttgg cagcaaacaa gcagaaattc tgtttatacc ttatgcagct gttactttct
      181 catacgatga atatgaaaaa aaggtgagtc agaagttcat tgaaggaggt taccaaatca
      241 aatcaataca tcatttcact gatccagttg ctgcaataaa aaaagctgaa gtaattgcca
      301 taggaggtgg aaatacctgg aaacttctga aaatgataag ggagaacaaa ctaattgaac
      361 cggtaaggga gaaagtattc tcagggactc catatattgg atggagtgct ggtgccaatg
      421 tcgcatgtcc gacaatccga acaaccaatg atatgccgat tactgaaccg gattcttttg
      481 aagcattctg ccttattcca tttcagatta atcctcacta cctcgacgtt aatcccgaag
      541 gttttgccgg tgaaacccgc gaacaaagaa tacgggagtt tatagaatta aatccgggaa
      601 tttatgtggc tggcctgaga gaaggcacaa tgctgctgat tgaaggcaaa aatgtcaaac
      661 ttataggacc caaaagcgct cgaatattca ttaaaaatgc agaacctttt gaagcaggca
      721 ataatgatga cctctcattt ctccttcaaa agaacatata atcttgattt aacaataaag
      781 taaactactg tcagttaaaa tagtttccaa atgaagctgg ttgtgaaaat atttgcatgg
      841 attgtagcag gaataattat tactctgact gcaatgatag taaccgcatt tgttttccag
      901 aaccaggcag tagttgccgt gattgattca atcaacagga atctgtcaac aaaattcgag
      961 ttcgaaaaat acagactttc tctgataaaa cgtttcccaa aagcttcatt tgaactactg
     1021 aaccttaccg ttttttcttc cgaatcattc gacaggactc aatggacagg aattaatact
     1081 gacactctgc taagggctgg tactgcatca cttgaattta aaattaccga tatactcaaa
     1141 aaaaaatatt ctattgaaag cataacaata agcgacggag aactttttct tattaccgac
     1201 agcattggag gaattaattt taaaatatca gattcaaata aagggaacta tgataatgaa
     1261 attgaccttg ataaaattgt aatacgcaat cttaaagcaa cttatttaaa caaggccacc
     1321 gacatttcaa tttccggtgt tataaataat ggaaaattac gaagcagaat tggtggaaaa
     1381 aatctcgact tttcatgcac atcaggaatt cttgttaatc aattcaatat tggttcatta
     1441 aatataaaac ctgacattca ggtatatgca gacattaatc tgagaaaatc agatgaacaa
     1501 ttaacttttc aaaacagtat tctaaaattt gagggccttt ctctcaggct aacaggttat
     1561 ataaaaccag aaaatgaact tttactgaaa attgagggac aaaacattga cctttcaaaa
     1621 gcaaagaaat acctcccaca gaagcttatt gaaaaatata ctgattttac tgcaggtgga
     1681 ttactgaacg ccagctgcat tttatcaggt ccattaaacc gcacaaagaa tttattcacc
     1741 caaatggcat tttcaattga taaaggaaag ctttacaata gaaaaacaga tatactggtg
     1801 gaaaatattt ctttttccgg aaattttaca ggtggagatg gttctgacta cccgagaggc
     1861 aaacttaata tacagccatg ttcatttatg attggaaatg cagaatatta tatttccttt
     1921 tctgcagaaa atcttaaaaa tccttttatc aaacttaact tttccggaga aattgttcca
     1981 gaagatttaa aaaaacttgt aagcatacca tggctcatat ccggagaagg gtctgcacga
     2041 ttaaacctga aacttgaagg tccattgccg aaaaaaacca aaaagctaaa tgcggctgat
     2101 attttcaatc ttaatccaca ggcaaatatt cagtttaaat ccctttcaat aaatcacaaa
     2161 aatgaaagat tttcattcaa tgatgcctca ggtaacattt ttatcgcttc atcattatgg
     2221 accgacgacc ttgtgttttc ctataataac cagcgtatta agattacagg agaatttggt
     2281 aatctgccag catggctggc aggaaaacca gaaataatta aaatcaatgc cagtataaca
     2341 gctgataaca ttgacgtaat aaacttttct gccagtaatt ctacagataa aagtaacatc
     2401 aaaagagcct taaaaatgcc tgaaggaatt gaggctgata tttcttttgt tttaaacaat
     2461 ttcaaatgga gaaaatttac tgccgctaac ataagtggga ctttaaatta taaacctggt
     2521 ttgctcaatt tcaccaattt ttctgcaaca acgcttgacg gattagtttc aggcattttt
     2581 gttctgtccc gtcaaaaaac aggtttacca tttgcttccc agggcaattt ttttattgaa
     2641 aatatcgaca ttaataagtg tttcagctcg tttgacaact tcgggcagga ctttattcat
     2701 tctgaacatc tttcgggcaa tctttcaggt acgttaaaaa taatgatgca acacgactcg
     2761 ctgttgaaac ctgatatcag gtcagtaaat gctgaaggca actatttgat tgagaacgga
     2821 tctcttacta attttgaacc ggtaaaagct ctgtcaaaat ttataaatat aaatgagctt
     2881 gaaaacattt cattttccag attggaaaac aatttctaca ttaaaaacaa ctatcttgct
     2941 ataccacaaa tggagattaa ttcttctgct gttaatttca ctgtaagcgg ccgacataat
     3001 tttgacaaca catatgaata tcatgtaaag gtttatctct ccgaattatt atcaggaaaa
     3061 atgaaaaaaa accagacaat aactgatttt ggtgcagttg aagatgacgg gctcgggcgt
     3121 acatctgtat ttctgaaaat aaccggtcaa ggaaacaaca tcaaagtttc acctgattta
     3181 aaagctacag gtgtaagaat cagggaaagt cttaataatg aaaaaagtaa tctgataaaa
     3241 attcttaatg aagaacacgg tttattcaaa aaagatactg ctatgaaaac agagcccgca
     3301 ccccggccaa aatttaacat agaatgggaa gagacaaata atgttgaaat taaaaaggat
     3361 acatcaggca accaggaaaa aaataatatt ttaaatcgta tttttaaaaa aaaatgaaaa
     3421 agataactta atcactctgt aaattgaaca tttattccca taagactctc tggaaactta
     3481 ttttactgtt tcttgcagta gggataggaa tgttttcatt ctgttataca tacttacttg
     3541 tcagaaatct taaatcagaa gaaaggaaaa aaattgaaat atgggctgaa gcaatacgtc
     3601 agttgaattt tgctgctgat acaggacaga cactcgaatt tttatcttca attattgaag
     3661 gtaacacaac aatacctgtt attcttgccg atgggaagga ttcaataatt tctgcaagaa
     3721 attatgatcc catgaaactt actgctccga acttccttca gacaaggctt aaaaaaataa
     3781 aggaaaagtc ggaacctgta ataatatacc tgcccgatgg ccatattaac aaggtttatt
     3841 atgaagacag tattattctc agaaaactga tatattatcc cattgttcag ctcagtatta
     3901 taattctgtt tatacttgtt tcttatctgg cattcaattc ctcaaggaaa gcagaacaga
     3961 accaggtatg ggttggcatg tcaaaagaaa ctgctcatca gcttggtaca cctgtttcgt
     4021 cacttaccgg gtgggttgaa atgctcagaa gcaaatatcc cgaaataccc ttcaccgagg
     4081 aaattgcaac tgatatcgcc aggcttgaaa aaataactga acgtttctca aagattggtt
     4141 cacgtccgga acttataagt acaaacatta ttccggtact acgtcaaacg attgattatt
     4201 tgagatccag ggcttcttca agaactgttt ttattaacga atatgatcct ctggctgaaa
     4261 tagtggttaa atttaatcca ccccttatta gctgggtaat agaaaatgtg tgtaaaaacg
     4321 caattgatgc aacaaatggt gagggaacaa ttaatatatc agttaccgaa atgcgtaact
     4381 acgttatcat tgatatcagg gacaacggca aaggaatacc aaggtcggct cataaaaaaa
     4441 tattcagtcc gggttataca acaaaacaga gaggatgggg ccttggtctc tctctttcaa
     4501 aaagaattat tgaagagtat cataaaggaa aaatatttgt taaatattct gagcccggat
     4561 tgggaacctg catccgtata atgctgagaa aaaactgaat tttacctgat aattataact
     4621 tgataaaacc agacggggat gaaaaggcat caagttgaaa tatcggctcc agccggttca
     4681 tgggcttctc ttactgcagc tgttcagggg aatgccgatt ctgtatattt cggcgcagaa
     4741 cagttcaata tgagagcctc atcatctgag aatttctcat tatcagacct gcctgagatt
     4801 gtttcattct gcaaatcaca cggcattaaa tgctatctcg cacttaatat tgtgatttat
     4861 gatgatgaaa ttaacaaaat gaaaaaaatt attgatacag cagctaaaag cgggatatca
     4921 gctataattg ccactgatca atcagttatc aattacgcct tttcaaaagg attggaagtt
     4981 cacctgtcta cacaacttaa tattaccaac accaacactc tgaagttcta ttcacaatgg
     5041 gctgatgttg cagtattggc cagagaattg tcgctcgaac agatcaaaaa catatcagat
     5101 gaaataaaga agagaaaaat atgtggtccc tccggtaaac cttttagaat tgaaattttt
     5161 gcacatggtg ctttatgtat ggctatatca ggaaaatgtt atctgagtct gcatgaatac
     5221 aataagtctg ctaacagggg cttatgcaat caggtttgca gaagatcata tattgtgact
     5281 gataaagaaa ccggatttga actggagatt gacaatcagt acataatgtc agcaaaagac
     5341 ctctgtacaa taggatttct tgacaggata atagaagccg gtgctgaaat acttaagata
     5401 gaaggccggg cacgctctcc agaatatgtc agaacggtga catcatgcta tgacgaagca
     5461 ctaagagcca ttgaacaggg cacatatact cctgaaaaca tcaaaaagtg gacagaacgt
     5521 ctttccactg tttttaacag gggattctgg gatggttatt acctgggacg taaaatgggt
     5581 gagtggacta acaaatatgg ttccagtgca accatgaaaa agacttatat cggaaaaatc
     5641 accaactatt acaacaaaat aggagttgct gagattaagc ttgagaacgg cgatttgtcg
     5701 ataggtgata caattcttat aatcggtcca acaacaggga cccttgaata tcgggtagaa
     5761 gaaattcgtg attcagaaca catgattact gaaaaagcat tgaagggtac attctgttcc
     5821 attaaaacag atcattattt aagacggtct gataaagtct acaaatggga agaaagggat
     5881 aaagaataat aaaatttatg caccaatccg ttattttata aaatatttaa gaataattct
     5941 tttcaggtca tctaatctca ctggtttggt aataaattct ttaatcccgt acatttcagc
     6001 ctttttcctt gtttcaggca tattgtcagc tgttacagca acaatcagca cttcaggtcg
     6061 ggcttcaaat attcttcttg ctgattcata accatccatt tccggcatga agagatccat
     6121 gaaaataaca tcatattttt tgctgaaagc catctgatat ccttcataac cttcttcagc
     6181 aacatcacat ttatatccga ggctttcaat catttttgcc agaattttct gactcatttt
     6241 attgtcctct actatcagaa tattaagact tttggcctct tcaccctcat gaagtatcaa
     6301 tccgacaggt tcaatatgga aaacctcact cacagcattc tgaaattcat gtacatcaaa
     6361 aggtttaacc agataataat ccacaccaaa agtcaagcat ttaaggtagt tcccctttct
     6421 gtcgttggta ctgaccataa ataccctgta tcttgaagag aggttattgt cccagagcat
     6481 ttgtgctact tcaatgccgt caaaattttc atcgtctgta ataataatca gatcatattt
     6541 tattttcggg attgttgagt ttacaagaat ctggtttaat gtggtttttg tataagaagt
     6601 aactgaaatc tgcaggcctg ccctgtgaaa aattgaaaga agttcttcat ctcttcctga
     6661 aggtccggta attacaagag ttttaatctg gtcaatgctc ttgattgttg aagtgtcgat
     6721 atccttttta attctctcat ttgaatgcac ctgaatggta aatgttattt ttgttccatt
     6781 atctcctgaa atacctgagg gactaacagc agtaagagtt cctcccatca gttcaatcag
     6841 ctgccttgca ataagggtgg aaaacattga ttcattgtaa ttcccgggaa ctatactctc
     6901 aatattaaca atatcaccaa agattttttt cagctcagcc ttgtcaaatg cttttccggt
     6961 atccagtatt tcaaaaccaa tggtaattac tccgcttttt tctgatttct tcagaattct
     7021 aacatgaacc tctccgtttt ctgtattgct tgtcgaatga ttaattaggt taaccagtat
     7081 ctgcctcaat ctgtaagggt ctccaattac cgtttcagga acgctatcat caacttcaca
     7141 aagaattgta acatttcttt ctttagcaag aggctttgca agatctatag cgtaataaac
     7201 ttcttccctg atattgaaag ctgtctcttc aagtatcatt ttaccggatt caatcttaga
     7261 aaaatcgagt atatcattta cgattcctag cagaacttca gtagatcttc gtaaaagata
     7321 gacgatatct cgcacctccg gtgaaagatc atacttatta aggatgtccg acattccgat
     7381 tattccgttc aaaggagttc ttatctcata actcatcctt gcgagaaact ctgtttttgc
     7441 aatatttgct ttaacttctt gttttcttgc cgattccagc atagttacgt ctataagaac
     7501 ctcaagggag gctttttccc tctgataaac taccggtatg ctggtatggt acagaattaa
     7561 ttccccaaaa ggttttttaa ttacaagaaa ctggtctgga ttaaatgatt taccaagata
     7621 ttttgaaaaa taagcattat caattttaag agttgtttca gggaatattt tacccgtcat
     7681 ttcagcttca ttttcgtaag aataatattc tgctgccact ttatttgctt ttaaaatttc
     7741 ccttttgtcg ttataaataa ttatacctac aggcatttcc tcaataagtc ttctaagggt
     7801 attctctgaa tcatgaagct cctgtatttt gttttcctga atctttaaca ctctccagaa
     7861 aaccagaatg atcaactgta ttaataaaat tgtagctgtc acaataaata ccgaattcct
     7921 tataaggtat ttctgaaaga aatctgcagg agctgagaaa ataactccca gatccctttc
     7981 aagaagctgt gttgaataaa aagaagaaag tacctcaacc ctttcgttat taattatcgc
     8041 ataatgtatc agattggaaa caatcccttc cttcaaacct tctgttattt tccctttttc
     8101 tgtatattcc ggtaaatttt cattattgtc aaatacaact tccccgttct ctgtcaatac
     8161 ccattgccac tgatagtctt tcaggttata ttttgaaaaa agttttccga aatatttcct
     8221 gtaatcaaca gttgtaacaa tattcccgct tgctttgccg cctttaaaaa ttaccatgta
     8281 ataattaaac tcaccgttat tttcaataag tttttccttg ggttctatcg gacgttgatc
     8341 aagtgaaaca aactctcctt caatccattc attcttagtt tcatccttgg ataaagtata
     8401 ctcattctgt ttatcatcat acagccttat tttgacaaca aaatcacgat actttgagta
     8461 aaaaagcttc aattgttctg ttattctgtt tttaatttca ggttttgttc tgtcaaaaaa
     8521 ataataagca tcatttgtaa aagttatttg gccgagatcg cttgtaaagg cataatttat
     8581 actgtccacc tcaagtccaa ctattctaac ctgcctgtcg aggagctcaa tcatatagct
     8641 tatctgattg cggtacaggt ttctgtaata gaagaaatta attaacagaa tcaataacaa
     8701 tactacttcg gctattatca aaatcctctt cattactcct aattttcatt aatatatcta
     8761 atataatttc ccattaaaat tacctgtatt ttctgttttc atcatcaaga atatttaagt
     8821 aactccggta tctcataatg tctatttctc ctgattcaac tgctggtatg actgcacaac
     8881 ccggctcatt aacatgcaaa cagttatgaa acctgcattc ttttgatttt ctgaatattt
     8941 caggaaaaaa atggtaaatt tcgttcttat ccatatcgat tattccaaat ccccttaacc
     9001 cgggtgtatc aaccacataa cctccaaatg gcattggatg catttccgga aaagtggtaa
     9061 catgtttacc ctgttcatga taaggtgata attcatccgt tttaagttta agtgaaggat
     9121 taagcaagtt taacaatgaa ctcttaccca ctcccgaatt tcctgcaatt aaattaactt
     9181 tatctttcat cgcttccttt acgagctcaa cattttttcc ggttttaact gaaatgcagt
     9241 aacaatcata tcctatatca ctataaagtg aggcaaggta attcattttt gctgtgtcct
     9301 cctcatcata taaatcgatc ttgttgaaaa taattatgct tttgattctg taagcttctg
     9361 atgttgccag aaatctgtca atgaattcct gaagtgtttt tggcatacgt atggttacca
     9421 tcaggaaaac ctggtcgata ttagctgcta taatatgata tttttttgaa aggttcgatg
     9481 cctttcttat tatataattt ttcctctcct caatttcaga aataattcct gtttccttcc
     9541 cgggtaataa agaaaacttt actctgtccc caacagcaat aggattagtg gtttgaatat
     9601 cttttattct gaaccgtcct tttatagtac attcaatgtt ccttccatca ggtatcatta
     9661 ctttataacg gcttcctgtc gactttacaa caactcctgt ttccaagtta ttaattttat
     9721 ttcaaatgta actaaaagta ttaaaattca atatattaat tgtaattttt atggtataaa
     9781 tgaatcctga caatctccct tttttactat tttatttatt cccttactaa gttttctata
     9841 tttacccact gatctgaaaa aaaaggggtc tgttaaatat tcagacccct ttctgtttgg
     9901 ttatactttt taaccttcag ctgccatcag gaagaatggt ttcatttcaa agctgtgata
     9961 atgaggcctg aggcgttcgg tgttataata ttcctccaca ttcacaaact ttttctgtga
    10021 agtaacaata tcaaccggag cattatcata tcttccattt ctcaggacaa ccagcctgcc
    10081 atggatgcct ctaagaatca aatcaagggc aagattaccg aatgccatag gtactataga
    10141 atcaacagca tcaggatctc cagagcgtac cagatatcca agcttctggt ttataatatt
    10201 tattgttttc cccttattga acttcggtga aagttccttt atttttgccg aaacaagatc
    10261 accaattcct cccaattttg cgtggccata ggcgtctttc tccatatttt caaataccat
    10321 ttctccacct ttaaacattg caccttccga taccagcact attgaatacc tgctcggatt
    10381 ctcattccgg tcgtcaacaa gaagttttgt aagatgctcg atatcaaatt tatattccgg
    10441 aatgacacat ctgtttgctg cacctgccat tgtcggaagt attgaagtga atccagcata
    10501 tcttccgaag acttcaataa ccagaattct ttcatgtgag ccggcacagg ttctcaggta
    10561 atttgtcatg ttaatcgtac gtgtaacaca agtgctgaat ccgatgcagt aatcagttcc
    10621 tggaacgtcg ttatccattg tttttggtat tgcaacgact ttcactccct tggtgtaaag
    10681 atgaactgca taacttaaag tatcgtcccc tcctatcggt ataaggtaat ctatacctag
    10741 ccattcaagg ttcttaagta cttcaggtgt cagatcattc atgtcggcat tgtatgtact
    10801 cttaagatgt tcaggtacat cattcttcga catatggctt ggacgggtac gtgaagtatg
    10861 caaaaatgta cccccggtac gtcctgcctt gttcactatt tcatttgtaa gaacctggta
    10921 gcatcggctg ttatcagctt tattgtccct gataatttca gtgagaccag cccagcctct
    10981 tttcaggccg attactttaa atccttcacg gtttgccctg attgtgacag ctctgattgc
    11041 aggatttaat ccaggaacat cacctccacc tgtcaggatg cctataactc ctctttttgt
    11101 ttttatgttt gccatagatt cagtttttta tgtttaccat aattttttca aaccgctaaa
    11161 gtaaaacatt ttcatattat tgcaaatatc ggaaaatagt taaatttatt taattttatt
    11221 aagcctattg tttcatggat ttttcaggag tgacacatga tcgctaataa aaaagaaccg
    11281 tgtaataatt actccgtact gattactggc ggcagcggta tggttggaag atatcttaca
    11341 tcttttctgc ttgaaaaagg ttatacagta tcacacctgt caagagttgc taatctattt
    11401 ggaagagtca gagtcttccg ctgggatcca gaaaaggaaa tactggatcc tactgctctc
    11461 gaaaatacag attatatcat tcatcttgca ggtgcgaata ttggagagaa acgatggacg
    11521 gaaaaaagac gagaagaaat tattagaagc agggtggatt cggccaattt gcttttaaat
    11581 actgtaataa aaaaccggtt tccattaaaa gcttttatct cagcttcggc aacaggctat
    11641 tatggttcta taacctcaga gacaattttt actgaagaaa tgggtccggg agaagatttt
    11701 acaggcattg tatgccagaa gtgggaagaa gctgctgaaa aatttgcaaa atcagggata
    11761 agaactgtaa aattaagaac tgctgttgta ctggaaaaaa attgtgctgt tcttaaaaag
    11821 cttcagctaa ctgagccatt tggtatccta cctgttcctg gaagcggaaa acaatatttc
    11881 ccatggatac atattaatga cctctgcaga atatatatca aagctgttga agacagccag
    11941 atgaacggtg cctttaatgc ctgcgcccct gaacagatta cttttattga ttttgttaaa
    12001 aaatacgctg agcttacagg aaaaaagaag attattacaa aagtaccggc ggttttactt
    12061 aaaattgctt tcggaaaaat gtctgaaata attcttcaag gaagcagagt atcatcttca
    12121 aaattaattt cagcgggttt tactttcgat tttcccacca tggataaagc tcttaaaaat
    12181 atattaggtt cctgaacaga actttatctt cctcctatct gcatctcagc cactctgaaa
    12241 gtaggtgtgg caaaagtccg gttcatatcc aggtcgttcc cgaccatatc aattgcattt
    12301 agaatttcat ctgctttgcc tgctatggtt agccctctaa ccggatgaac tatttctcca
    12361 ttttcaatcc acaaccctga agctcctcca ctgaagttac ctgttacagg atcaattccg
    12421 taaccagtta cctccttcac gagaaaacct ctgcgtgtag ctgctattat ttcgttcttc
    12481 agacttttcc cagccggaac atatacatta tgcacgccta ttccaggcag agaactgaag
    12541 ccacctcttg ttgcatttcc agtacttttt acacctgccc tttttgcagc tttaagatta
    12601 tatataaaac tctttagaat gccattttca ataataatat tttttattgt tggaacacct
    12661 tcaccatcga acgggcaact tgcaagtcct tttggtttta ttccatcgtc aactattgtc
    12721 agaaggggtg atgcaatttt ttcgccgagt gaattgcgca gaaaacttgc tccctgaata
    12781 acccgttccc cattaatagc agagataatc cctccaagaa gagaccggcc gacatcagca
    12841 tcaaatatta cagttgccct ttgtgtgctg accataacgg ggtcgagcat ttcgaaagct
    12901 ttccgagcag ctactgctgc aatctcttcg ataggaagaa gatcctcaaa aaatacacgc
    12961 gaacagcttt cacctccagt ggttttctgc tcacctttct cagccacaac gcttacgccc
    13021 aaagagcatg aacttgtttt gtaattcttc agtataccat tagaattggc aatataaatt
    13081 tctgtctccc tctccctgta agatgaacct gaactttttg taattctctg atcctttaaa
    13141 gcttcctttt caagttcaat tgccatttta attttattgt caaccgggat attccccaaa
    13201 ttctgatcgt ataaaccatt aacctctgaa acagttttgt catcggtcag aacgttatac
    13261 tcatcaggcg tagaaagttt tgcaaactca attgccctta aaattgcctc ttcaactgat
    13321 ctgtcagaaa aattattaca atggctgaaa cccattctgt tacccgtaaa aaccctgaaa
    13381 cccacccctt gtgttgaggc ctcttcaatt gtttctattt cagaattcag aatctgtatt
    13441 gaaagatttc tttccgactg aaaatatacc tcagcaacat ctgccccaag tttcaggcat
    13501 tttttaagca gtttctcaac aagatttata taattcatat tcatatgttt ttaaaattca
    13561 accccttgta cctccaacgt ttattcctct tattcttact gtaggctgtc cagcggtaac
    13621 ttcggctgat tgccctttcc cgcatatacc aggaccaaaa tcaaggtcat ctccaactgc
    13681 atcaatgttt gttataacat ccatgcatga gcctattagt gtggcacctt taaccgggta
    13741 agtctttttt ccgttttcaa ttaaatatgc ttcccggcag gtaaaattaa atactccggt
    13801 tactggatta acgctgcctc cgctcaggct ttgcacatat aatccttttt tggttgaagc
    13861 cagaatatct tcaggtttgt ctttcccttt ctcaataaaa gtattggtca ttctgggaat
    13921 tggaatgttt ctgaatgatt ctcttcttcc gtttcctgtt cttggcaacg aaagttgttt
    13981 tgcactcagt acatcagtca tgtaaccttt taatatacca ttttcaatca acacgttacg
    14041 ttgtaccgga gtaccttcat catcaaaatc gattgtgcct ctgaagttgg gcaatgttcc
    14101 atcgtcaacc attgtaaaac aatcgcttgc aaccttttta ccaagctttc ctgtaaatgc
    14161 tcctacacct ttggcaatat tatcagcctc aagaggatgc cccactgcct catgcaccag
    14221 aacacctccc catccgtttt gcataaccac gtccatcact ccagcaggag catcctttgc
    14281 ctccagcatt gcaattgctt cccgtgcagc attttgtgcc actttttctg gtgtcacaag
    14341 atcaaacatc tcaaaacccg aatgataact taacctctct cttgccatat gtcttgaatt
    14401 accatcatca ctcattacct gcacaatgaa aaacaaaaga ggtaattcgt ttctgcagta
    14461 aattccttcg cttgttgcaa taatccttga ccttatctca tcattatact caataatggt
    14521 catttttatg cgcctgtcat acgaaagggc agcctgatca gctctctgaa tgatttccag
    14581 gcgcttttca tcagcaacat cctgcaatgg caccttcact gtaatatatg aagggttttc
    14641 tgcaacttcc accggtcggg ggattatcag gttaccgttt tgtgcaacgt atgaagctat
    14701 ctcagcagcc cgtaacagct tattgtaact tatctcttcc gtgtaagcat aaccggtacg
    14761 gtttcctgat ataaccctta ctcctgctcc ctgactgacc ccatataaac cactcttgaa
    14821 ttttgactct tccatcaaga tctgtcttgt tatccggttc tcaatgtaaa tatcagcata
    14881 atcaccgccc tttttcagag catgggcaat aactttatca agcatctcac gatttatttc
    14941 cggaggagga aacatattct gtttacccga acatgaagta agatactcaa tcatatacgg
    15001 cgaggcagct actataatac ctcccttaat cccaagctta agaaactttc ttctcggaat
    15061 atcggaaaca taggttcttt tcatatttta tttattctgt taatgatttt tctgattttt
    15121 cctgagttgt gaagatagtt atatcaattt aattattaaa agattgtttt acataaaatt
    15181 ccttgttttt taaaactcct cccctggtgg aataaacact cgcttttcca ctgaagtaac
    15241 tgttaccata aactctcact tcaaaaatca cagtagaaac agctcctcct tttgtatccg
    15301 gaacattttg tgaaatgctc ctcaacgcac ttctttgctg tagtacagga ctatctgaaa
    15361 aagcatctct gccagatcgg ttttctcttc cctgctcagg cgacggactc aatactttaa
    15421 atattcgctt atccccttta aaagcagctg aatcaatttc aattacaaca cggtcgtccc
    15481 ttacaatctt caccagatgc gcttgtttaa gagctgttgg aagtaaaccg gtattcttat
    15541 agctaactct aatctgataa tctgctgaat cctttctgta tgattttatc ttttttattt
    15601 caatattttc ccacttaagc tcaggcagag cttttattaa ttcaagatta aatagagctt
    15661 gatttgatac ccatttttca agatgtcggg gcggtggatt ctgagaaaag aattttggat
    15721 tgaatccgcc cagctcaaca tcaccgtaaa ctggatgcct ggcaggtttc cattcaataa
    15781 acccatcacc gttgttttct tcgtcatccc atttaagtat ttcactctgg tcaattgtac
    15841 cgtcaccgtt ataatctttg ttcctccccc cattccatat ttcatcaccg taccagattg
    15901 cgccaaagta ccagtatcca aaatcagggc cgtgtccaaa aagcggatta ccacggttat
    15961 aatcattaaa tacatcccct gctcttgaat atcctgttat tttcttgccg agctcatcaa
    16021 aatatctata ccagctcaag tcttcaggat acattctttc ttccgacgca gatgttgaag
    16081 gtgctcttag atgcatgggg acgcttgtat ccattgaatt tacaacataa acgttaggat
    16141 gtgacagtaa aaaagtaacc accgaccttg tttctgtttc gctgagcgga tattcacctg
    16201 cacccgactg cgtataacca cgaccggttt cttctgattg cggcctccag ttttccggat
    16261 aattcctgtg caggtccaac cctccgatgc catcttcatt cactcttccg tcaccgtcat
    16321 tatcaaaacc ttcctgtgca acaagataga tccccttacc tgcttgtacc tgtttcatta
    16381 atctgccagt tggatcagcc ggatcgggaa tatatgttcc cctcttctca tctttccatc
    16441 tcatatttaa tattaagcca tcaccgtcca gatcttcagg cgaatcttca tccagtaatc
    16501 cgtcaccgtc gttgtcaaat ggccttactg tacttcgatt gctttgagca gttttcaggt
    16561 aaaggttatg tccatccgga ttgtttacag gtcttatgta aatagttcgc gtatctacca
    16621 gatgagttat ttcaggatct ttcccataat tctcaaccag atatttaata agccataaag
    16681 cactctctga agctgtaatc tctccactgt gacgcccacc ttcaaaatac gccccaggct
    16741 tttcagtgtc tttcccacta ctcctggcag aaatagttac ctgatatata ggccttcctt
    16801 catagctgcg tccaacttcg taaagctgaa aaatgtcagg atacttttct gcccatcttt
    16861 caagccacat gtaaattact tccagagaat gatacctgtc aaaactcaat gtttcacctg
    16921 cttcatattc aacacctcta tatattactt caggaaaata acttactcca tgtctgaaac
    16981 cggtcaacag gtaattcgac tttttctctc tgccagttgc tgagtcaccc tgagagccaa
    17041 tattattata attctggctt atcagaaatc tgctggtcaa aagtaatact atcaaaagaa
    17101 ttatcttttt catctcgttt tttaaggcct gaatttaaat tattttatac tataatcaaa
    17161 tttcttgctg tttaaatagc gaggtgtata tttgcttcag atttatccaa tagaaataaa
    17221 ctgtaaaata acatttatgc tatatattac tgattatcac atccatacaa attacagcga
    17281 tggtactgca gaacctgaag aatatatttc aactgcaatt aaaaagggct ttaaagaaat
    17341 tgggttttca gaacacctta acctgttcag aacagatctg acgtggtgca ttgatatgca
    17401 gaaagttccc gattatatca gccacatatc aagtctgaaa aatcaattgg ataatattaa
    17461 aatcaaaaca ggtttcgaaa tagattatta ccctggcaag gaaaaggaaa ttaatgatat
    17521 tatttcatcc ctgaatatcg attatgcaat tggttcaatc catttcatag gtgaaaatac
    17581 cgttgacagc agtgaggact tttataatgg taaatcgttt aatgatattt tcgttcatta
    17641 ctttgaaatt gtttttgagg caattaaatc aggactgttt gattttattg ctcattgtga
    17701 ccttataagg attttcggcc tgaaatatca tggaaatccg gagcctctct acaggcatct
    17761 tgctcatctt atggcaaagc atgaagtagc actggagata aatacaaacg gaaaaaacaa
    17821 acctctaggg gaattttatc ctgatgtaag gttcctccat ttattcaaaa aagagaatgt
    17881 acccgtatgt gtcaattcag atgcacataa tcccgggcgg ataggtgaat atttcaatga
    17941 agcttaccag ttgcttaaag acgcaggata tactgagatg tgtactttca acagaaggga
    18001 aagatatatg atacctgttg atttcagtct tttatccacc tgataggtgt tcagtaaata
    18061 ttttaactcc tatccctata agaataactc cacccattat ttctgcatat tttccagttt
    18121 tttctcc
//
LOCUS       DAOSNB010000015        18007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3137_length_18007_cov_4.883857, whole genome shotgun sequence.
ACCESSION   DAOSNB010000015 DAOSNB010000000
VERSION     DAOSNB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3137_length_18007_cov_4.883857"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLG42_01460"
     CDS             <1..648
                     /locus_tag="PLG42_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU29908.1"
                     /translation="VVQPEVRMYFNPSLKSHFMFVPGVITLIMILICALMTSVTITRE
                     KEFGTMEVLLVSPLKPSQIILGKVAPYFLLSFINIITILILSWFVFGLPVRGSLILLL
                     LETMLYIMMSLTLGILISTVSKTMQQAIFISAIGMMLPTILLSGFIFPIENMPKVYDY
                     FSSILPPRYFIVIIKNIMIKGTGLQYVWKETLIIALMTLIFMALSIKKFSLRLQQ"
     gene            653..1771
                     /locus_tag="PLG42_01465"
     CDS             653..1771
                     /locus_tag="PLG42_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU29909.1"
                     /translation="MRTILFLIRKEFLQIFRNRFIGRAIFAIPLIQMLILVPAVTFEI
                     KYVNLCIIDNDRSVESRELIGKLEGSPFFRVKYYTWSEEKANELIYNNKCSAIINIPS
                     GFGKELGAGRPVKILARIDAINAMKGQLTWAYLSGVISSYNENIIAESLPVVQATMVP
                     RISVTGRYWYNEMLNYKYYMLPGILVILVTAIGFMLAGLNLVREKEIGTIEQINVTPV
                     KKSEFIISKMVPFFIIGLADLGFGLILGKIFFNIPFEGSLLLMFLGSAIFLVAVLGLG
                     LFISTFSSTQQQFMFVAFFFIIVFVLMSGIFTPFESMPMWAQKFDLINPVAHLIRFNR
                     MVMLKGSGIADVARELLLLCGIAVMFTTFAVRRYRKTA"
     gene            complement(1784..2644)
                     /locus_tag="PLG42_01470"
     CDS             complement(1784..2644)
                     /locus_tag="PLG42_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU29910.1"
                     /translation="MLRTSVILFLAIGFLVSCSKDKSNTLKVITFNIRYDNPRDSINA
                     WPNRKEFLISFLKEEKPDLFGLQEALWHQYAAIDSAMDEYLSVGVGRDDGDKKGEMNP
                     VFINGKRFEIVRSNTFWLSLTPEVPGSKGWGASLPRIVTWVELKGKKDGRTLYYFNTH
                     FAHDSDSARVMSAGILLNEVRKIAGDNQFVITGDFNMTPDSRAYSILTGAGSENPLII
                     DSYIITETPPAGPVGTFNGWSDTPRGNRIDYIFVRNGMKVKSISTIVKKKGPVFISDH
                     WPVVGEIYLN"
     gene            2732..4543
                     /gene="mutL"
                     /locus_tag="PLG42_01475"
     CDS             2732..4543
                     /gene="mutL"
                     /locus_tag="PLG42_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOU29911.1"
                     /translation="MPDVIRLLPDSVANQIAAGEVIQRPASVVKELMENSVDAGARSI
                     KVIIKDAGRTLIQVIDDGTGMSETDARLAFERHSTSKISCAEDLYAITTKGFRGEALA
                     SIAAVSMVELRTRRTEDETGTLVVISGSKVERQEMCSCPAGANFAVKSLFFNVPARRK
                     FLKSDNTELRHIISEFQKIAIAHPEIKFSLTHNDTEIYNLPAANTRQRIAAIFGKNTH
                     QELLNLETRTSLADITGFIGKPESARKTSGEQFFFVNNRYIKHPYFHKAVIEGYQKIL
                     PLDVVPSYFIFISVDPSRIDVNIHPTKTEVKFEDERAIWQILLAAVRETLGRFNIVPA
                     LDFESESYFEIPVRNSNSPLPSPPEIKVDTSFNPFGEDRKPSRFELVEKFEKERLAYW
                     EKLYPEKKEPVSRPQQPFETGFRKEERRVLQIKGRYIVCPVKSGLMFIDQHRAHERIL
                     YERFIECLDNRSVPGQKTMFPEKIELDASAFYTLKEIEDELRAIGFDFEFGDNFNVEL
                     KCYPSLEISDRGTELLKTLLDSFKSLQSDPTADLREKVAIAMAQTAAIPYGKTLTKEE
                     MEQLIDTLFACKLPNYSPSGKPVVSIMTTEELDKRFK"
     gene            4659..5372
                     /locus_tag="PLG42_01480"
     CDS             4659..5372
                     /locus_tag="PLG42_01480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU29912.1"
                     /translation="MPPVVKNIIYINILMLLAYYAAASVFDIDLNRILGIYFPKSDQF
                     RPIQILTHMFMHGGFWHLFFNMFALYMFGQVLEQVWGPRRFLIYYFVCGLGAMATHMA
                     VMAIQYYRVLAYITPDQLQEVIDNGYVYSLQMKAYLDPAMKELQTILYTPTVGASGAV
                     FGVLLAFGVLFPNTQLMLLFPPIPMKAKYFVMLYGGLEFYMAITQPGSSVAHAAHVGG
                     MIFGYILIVIWRKTTKTLY"
     gene            5372..6265
                     /locus_tag="PLG42_01485"
     CDS             5372..6265
                     /locus_tag="PLG42_01485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU29913.1"
                     /translation="MSLWSDIRSTFRHGTSLTRLIILNIAVFLAISVIGIIGFLLANP
                     EISDYTIRLFSVPSSFKYFILRPWTVITYMFTHKDLLHIAVNMLWLYWFGKIFLDYLD
                     QKKLVAVYLLGGITGALLYVISYNIFPAFSEFAGSSIPLLGASAAVMAVVVAISVYVP
                     DYTVFLFLLGRIKIKYIALVIFILTSIVDFQANSGGKLAHIGGAIFGYIYTVYYRKGK
                     DLGKWLNRIIDFFVTFLKPRKKLKVTYKKRVTDDYEYNRIKAERQARINEILDKISKG
                     GYDSLTREEKDFLFRESQKKH"
     gene            6268..7386
                     /locus_tag="PLG42_01490"
     CDS             6268..7386
                     /locus_tag="PLG42_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU29914.1"
                     /translation="MVKSKTSLRFPGVRRLIHNIFLSLNIFFALSLIFSYLAVHINPG
                     DFSIPAFFGLAYPYLLLINIVFLVIWAALLRWEALISFAVIVAGYSHFSNYIRIFKPH
                     NTENGNILIMCYNLRLMNYYEDPQERETEKKILALIKSKQPDILCLQELFINGDASQK
                     DNEIKKALGGGYYSHTKLLGKGKNKFYGIATYTKYRIAGTGEIIHPGSSSLSIYTDVI
                     IQGDTFRVFNNHLQSFRLKKTEKSFLEELLEAENREAVNEIMSLSASLKKGFIQRALQ
                     AQVVKDHINRSPYPVIVAGDFNDTPVSYSYRKIRKGLNDSFVTSGYGAGFTYRGNYPP
                     NRIDYILYDNSLVNTDFEILRVKYSDHYPVMAWFRKAD"
     gene            complement(7370..8323)
                     /locus_tag="PLG42_01495"
     CDS             complement(7370..8323)
                     /locus_tag="PLG42_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOU29915.1"
                     /translation="MILPSLENIKEAHRRIKSYVHRTPVMTSTQINRISGAEIFFKCE
                     NFQKVGAFKFRGAMNAVLLLKEEEVREVITHSSGNHAAALALAAKMAGMKAYVVMPRT
                     APSVKINAVRDYGAEITFCEPTQKAREEATGILMEKTGARLVHPYDNFNVICGQGTAA
                     VELLEEAGELDILIAPVGGGGLLSGTSTAAKGLKSNIKVFGAEPRNADDAYRSFTTGI
                     LHPSVSPKTIADGLLTSLSELTFTIIRKNVDNIFTVSEESIIEAMRLIWERMKIIVEP
                     SSAVTLAVVMENPDVFRGKKTGIILSGGNVELSELPFNQLS"
     gene            complement(8408..10699)
                     /locus_tag="PLG42_01500"
     CDS             complement(8408..10699)
                     /locus_tag="PLG42_01500"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOU29916.1"
                     /translation="MVSSIFWIVPVAALTALLFAWIFYRSMIVREEGTDRMKEIAQYV
                     REGAMAYLKRQYTVVTKVFLILVVLLFVLAYFGVQNPFVPVAFLTGGFFSGLCGYLGM
                     RTATYASSRTAWAASTSLNKGLQVALRSGAVMGLVVVGFGLLDIALWFVILNYAVFTP
                     DNMEHGLKFLGLTFVAPGTTEHQKLIEITATMLTFGMGASTQALFARVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGA
                     ALPSLDAENQIKAIIAPMIVSAVGVVLSIMGVYMVRAKESATQRNLLRALLLGTGGSS
                     ALILVVLAVMAFTGWITWGVFGSVIAGLIAGVIIGQATEYFTSDEYKPTRGIAKQSQQ
                     GPATVIIEGMAVGMMSTWVPVVTIVAGIVAAFGFAGGFHNFAQGVYGIGFAAVGMLST
                     LGITLATDAFGPIADNAGGNAEMAHLPKEVRERTDALDALGNTTAATGKGFAIGSAAL
                     TAMALLAAYIEEVKMWLGKLADKAPEGYKQVGDILFYKSHAPAVIQEGQRAVQVTQAH
                     IGDFVAAYNISLFNPILLGGLFIGAMMAFVFCSMSMKAVGRAAGRMVDEVRRQFREIP
                     GIMEGTGKPDYAKCVEISTRGAQREMLAPALMAIIVPVLIGVIFGVPGVVGLLAGGLT
                     AGFTLACMLNNAGGAWDNAKKYIEKGNYGGKKSDPHKAAVVGDTVGDPFKDTSGPSLN
                     ILIKLMSMVSVVMTGLTVAFKLL"
     gene            10984..11967
                     /locus_tag="PLG42_01505"
     CDS             10984..11967
                     /locus_tag="PLG42_01505"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOU29917.1"
                     /translation="MKPERKVRIGIAGYGNLGRGVEYAVKQNPDMELIGVFTRRPPEK
                     LHCSLPVFHISDMKNFKDKIDVMILCWGSATDLPEQGPAAAEIFNTVDSFDTHAKIPE
                     YFIKIDECGRKNNTLSLISSGWDPGLFSLARLLGHCVLPEGKDYTFWGKGVSQGHSDA
                     IRRVPGVRYGIQYTVPVQDVVEKIRNGEEPELKSSDMHLRICYVVPEKGADLKKIEET
                     IINMPHYFKEYKTTVNFISEEEFKKNHTGMPHGGTVIRSGKSATGLKHRIEFTLNLES
                     NPEFTASVLVAYARAIFRMAKEGKTGALTVFDIPFAYLSPEPDEKLREKLL"
     gene            12035..>12415
                     /locus_tag="PLG42_01510"
     CDS             12035..>12415
                     /locus_tag="PLG42_01510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU29918.1"
                     /translation="MVKISAVVIAFNEEEFIGRCIESLKEVADEIIVVDSYSTDSTPD
                     ICRSLNVKLVQHPFEGYAEQKNFATSLATYPVVLSLDADEALSEELKSSILKVKEDFR
                     YDGYYFNRRNNYCGKWMSHSRLYPD"
     assembly_gap    12416..12515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12516..12704
                     /locus_tag="PLG42_01515"
     CDS             <12516..12704
                     /locus_tag="PLG42_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29919.1"
                     /translation="HFHFAWNFFRSFILSRGFLDGYYGYTYCMITAFGSFLKYAKLRR
                     LWLLKKQSDSRKNNEKGS"
     gene            12688..13251
                     /locus_tag="PLG42_01520"
     CDS             12688..13251
                     /locus_tag="PLG42_01520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU29920.1"
                     /translation="MKKDLDISLDVLRKGGVILYPTDTVWGLGCDATCAEAVEKIFAI
                     KQRRDSKSLIILVNSHEMLEEYVQEIPPMAEEITKIADKPITIVYPRSRNLAPGVSAE
                     DGSVGIRITKDKFCSALIEKLGKPLVSTSANRSGEPAPGNFSEIPEEIIKAADYTVTI
                     RREEKRKAKPSPVIRVEMNGEIKILRR"
     gene            complement(13270..13791)
                     /gene="rimM"
                     /locus_tag="PLG42_01525"
     CDS             complement(13270..13791)
                     /gene="rimM"
                     /locus_tag="PLG42_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOU29921.1"
                     /translation="MEEYILLGKILKLHGISGVVAVSTDKPLSKAFSKPDFVFLEIEG
                     KKVPFCVETCELQNNKTLFLKFEGYGSAGKVKEFTGCNVYVKVSGSRKEKKVFYWDEF
                     LNYRIISEGSGISGNIIAVIDNPGNLLLKVKDENSGSELFIPFHEDLIVAINKKQKII
                     YMNLPEGLSDINK"
     gene            complement(13836..14387)
                     /locus_tag="PLG42_01530"
     CDS             complement(13836..14387)
                     /locus_tag="PLG42_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOU29922.1"
                     /translation="MAVRIRLARKGRKKLAYYHIVVADSRSPRDGRFIEKLGIYDPRT
                     DPATVELDFDKSLDWLQKGAQPTETCRAILSHKGVLLKKHLLEGVKKGAFDEAEAERR
                     FNEWLKQKEAKIQEKIQKLEKAADEELRKRIEEEKKINEKRAAELAKKNAEKNAEMAA
                     SAAEAEPSTEESSASDTETSSEQ"
     gene            complement(14522..15877)
                     /locus_tag="PLG42_01535"
     CDS             complement(14522..15877)
                     /locus_tag="PLG42_01535"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU29923.1"
                     /translation="MNRSIILTTVLTLFMLLTGCDWLLEPEPSQSVSEEVALSTDANV
                     KSILNGAYSLFNEPGIYGGNILRNAELLAGNGEILWVNEETYYEPFEFFTKTISADNS
                     EVFTQWRESYRVINTVNNVLSALDVVKKADRGWVEGEALFLRALIYFDLVRFFALPYE
                     PGALNNQYGVPLILKPIREISDEVYTGRNTVEEVYNQIIKDLVAAAERLPDDNWIFAT
                     RGAANALLARVYLQKGDYDNARKAADAVISSGKYNLLPVYASIFNQYEPTKEDIFVAK
                     ISYPTEYNAMTVSFSTSSFGGNGFIEILDGHLNIYPKEDKRAELFYTGDGALRCGKWN
                     NLYGCVNLIRLAEMFLIRAECNLRLGPPYVGASPVDDYNAVHTRAGLPAAGSVSLDEL
                     ILERRRELAFEGHRIHDIKRLKQNIGPWNYKNPKLVFPIPAREILNNPALQSQQNEGY
                     R"
     gene            complement(15896..>18007)
                     /locus_tag="PLG42_01540"
     CDS             complement(15896..>18007)
                     /locus_tag="PLG42_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU29924.1"
                     /translation="VSTDWQSLAFRKGRIWSADLSAQGGNDKLQYSAGSSVLINDGIL
                     VSNGIEKISARLRIDNKVSRRIDLGFSLRINRAAIDQVSSDNYLSSPLQLVAQAPVTP
                     PRDADGELSTFPVTSYYNALLDAEYASKKITEYRSLTSGYINFNIIEGLTWKNELGFD
                     LYGNKENARYGEKTMEGMDRSGYGFANHGQNQNITGRSLLLFNRKLSNISLNTLLGTE
                     FQHTSVETMYTEGERFASDALKTIASAGIISGGSSTLSRYGFLAGFLRVNTNYDGKYL
                     LSLSGRIEGSSRFGSNNKFGFFPGISAGWIMTGENFMQSVPFLSFLKIKAGYGLAGNA
                     GFGNYWHLGLYDSGSYGDVPGLVPGQIANPDLKWESLSEINTGVEFGFLKDRISGEID
                     IYQRKATNILLGEEVPSTTGYLTRIQNSGAIQNKGLEFVLNTVNITGLFEWKTSLNFS
                     LNKNKVLSLGKQNILDFGGESYMNVAMTGYPIGSFYGAEYAGVDYNNGDALWYINEKD
                     ANGNIINPDKTTSNFNSANFIILGNPNPDFMGAITNTLCYKGLELIFTFQGVAGNKIH
                     LAGDRWMASNGVWFDNQLKSQLRSWKSKGDITDVPQARLLWDNGNQSRSSRYLSDGSY
                     LKLREVSLSYDVPSVVTNKLNIRKMQLFIQGRNLFTFSLYEGWDPEVSADFMVNNIFS
                     GFDFYSAPQPRTIVFGITLGF"
BASE COUNT         5233 a         4017 c         3496 g         5161 t
ORIGIN      
        1 gtggtccagc ctgaagtacg catgtatttc aacccctccc tgaaaagcca tttcatgttc
       61 gtacccggcg taatcacact tatcatgata ctcatatgtg cactgatgac ctcggtaaca
      121 ataacgcgtg aaaaagaatt cggaacaatg gaagttcttc ttgtctcacc actgaaacct
      181 tctcagataa tactcggaaa agttgctcca tattttcttc tctcttttat aaacatcatt
      241 acaatactca tcctttcatg gttcgtcttt ggcctgcccg taagaggaag cctcatatta
      301 ctgctacttg agacaatgct ttatattatg atgagtctta ctctgggcat actcatttca
      361 accgtttcaa aaaccatgca acaggcaata ttcatctctg caatagggat gatgctgcca
      421 acaatactcc tgtcgggatt tatcttccca atagaaaaca tgccgaaggt ttatgattat
      481 ttcagcagca ttctgccacc gagatatttt atagttataa tcaaaaatat tatgattaag
      541 ggtaccggac tgcaatatgt atggaaagaa acattgatta ttgctttgat gacattgatt
      601 tttatggcat taagtattaa aaaattcagt ctgagacttc agcagtgagg atatgagaac
      661 aatattattt cttataagaa aagagtttct tcagatattc cgtaacaggt ttatcggcag
      721 ggcaattttt gccattcccc ttatacaaat gctaatcctt gttcctgcag ttactttcga
      781 gatcaaatac gtaaatcttt gcattattga caatgaccgc tcagttgaat cgcgggaact
      841 tattggcaaa cttgaagggt cgccgttctt cagggtaaaa tattatacat ggtccgaaga
      901 aaaagccaat gagctgatat acaacaataa atgcagtgca ataattaaca tcccctcagg
      961 tttcggcaag gaacttggag ctggcaggcc ggtaaaaatt cttgccagga tagatgccat
     1021 taatgcaatg aaggggcaac tcacctgggc ttaccttagc ggtgtaatca gcagctataa
     1081 tgaaaatatc atcgcagaga gtctgcctgt tgttcaggca acaatggtac cacgcataag
     1141 tgtgactggc aggtactggt ataatgagat gcttaactat aaatattaca tgctccctgg
     1201 tatcctggtc atacttgtca ctgccatagg atttatgctt gccggactta atctagtcag
     1261 ggagaaggag attggaacaa ttgaacagat aaatgtcacg cctgtgaaaa agtcagagtt
     1321 cattatctcc aagatggtgc ctttttttat tattggcctt gccgatcttg gtttcggact
     1381 catcctcgga aagatatttt tcaacattcc gtttgaaggg agcttattgc taatgttcct
     1441 gggttctgca atatttcttg tagcggtact tgggcttggt ctgttcatat caaccttttc
     1501 atcaacacag cagcagttca tgtttgtggc atttttcttc ataatagtat ttgtactgat
     1561 gagcggtatc ttcactcctt ttgagagcat gccaatgtgg gcccagaaat ttgatttaat
     1621 caatcctgta gctcacctga taagattcaa ccgcatggta atgctgaaag gttcaggtat
     1681 tgctgatgtt gccagggagt tgctcttgct ttgcggaata gctgtaatgt ttaccacatt
     1741 tgccgtgaga agatacagaa aaactgcctg aagtatccct catttaatta agatagattt
     1801 cacccacaac cggccagtgg tcggatatga acaccggacc ctttttcttt actatagtgg
     1861 aaatgctttt caccttcatt ccattgcgga caaaaatata atctatgcgg ttccctctgg
     1921 gcgtatcact ccagccattg aatgtaccaa ctggacctgc aggtggtgtt tcagtgataa
     1981 tgtatgaatc aattattaaa ggattttccg aaccggctcc ggtgagtata gaatatgcgc
     2041 gactgtcggg tgtcatgtta aaatcgcccg ttataacgaa ttggttatcc ccggctattt
     2101 ttctaacttc attcaagagt attccggcac tcattaccct tgccgagtct gaatcgtgtg
     2161 caaaatgtgt gttaaaataa tataaagtcc tgccgtcttt cttgcctttg agctcaaccc
     2221 atgttactat tctcggtaat gaagctcccc agcctttgct gcccggcacc tcaggcgtca
     2281 gcgaaagcca gaaagtatta ctcctcacta tctcaaacct ttttccattt ataaataccg
     2341 gattcatttc gcccttctta tcaccgtcat cgcgtccgac tccaactgaa aggtattcat
     2401 ccattgcaga atcaatagcc gcatattggt gccacaatgc ttcctgcaga ccgaacaggt
     2461 cgggtttctc ttctttcaga aaacttatca gaaattcttt tctgtttggc catgcattaa
     2521 tactgtcgcg aggattatca tagcggatgt tgaatgttat tacctttaga gtgttgcttt
     2581 tatctttact acatgataca agaaagccaa ttgccagaaa aagaattact gatgtcctta
     2641 gcatattttc atttttcccc aaagataaca acaatgaatt aattactgca gccgcgataa
     2701 taattaatat atttgcacaa taacacttat catgcccgac gtcatacggt tacttcctga
     2761 ctctgtcgcc aatcagattg ctgctggaga agtgattcag cggccagcgt cggtcgtgaa
     2821 agaattaatg gaaaactccg ttgatgcagg tgcaagaagc attaaagtta ttattaaaga
     2881 tgccggaagg acacttatcc aggttataga tgatggcacc ggcatgtcag aaacggatgc
     2941 cagactggca tttgagcggc attcgacatc aaagatatca tgcgctgagg atctctatgc
     3001 aattaccacc aaaggcttca ggggtgaagc tcttgcctca atagcggcag tctctatggt
     3061 tgaactccgc acgcggcgga cagaggacga aacaggcaca ctggttgtta taagcggctc
     3121 gaaggttgaa aggcaggaga tgtgcagctg ccctgcaggt gcaaacttcg cagtcaaaag
     3181 ccttttcttc aacgtgcccg cacgaagaaa attcctgaaa tcggacaaca cggaactgag
     3241 acatattata agcgagtttc agaaaatagc tattgcccat cctgaaatta aattttcact
     3301 cacgcataac gatacggaaa tatataacct gccggcagca aacacccgtc agcggatcgc
     3361 tgctatcttt ggcaaaaaca ctcatcagga actgcttaac ctcgaaacaa gaacatccct
     3421 cgccgatatc acaggtttca tcggaaagcc cgaatcggcc agaaaaactt caggcgaaca
     3481 attcttcttt gtaaataatc gctatataaa acacccatac ttccataaag ctgtaataga
     3541 aggataccag aaaatactac cgctcgacgt tgttccctca tatttcattt ttatctcagt
     3601 cgatccttcc agaattgacg tgaatatcca ccccacaaag acagaagtga aattcgaaga
     3661 cgaaagagca atatggcaaa tacttcttgc cgcagtaaga gaaacactcg ggcgtttcaa
     3721 tattgttcct gcactagatt ttgaaagtga atcgtacttt gaaataccgg tcagaaacag
     3781 caactccccc ctgccatcac ctcctgaaat taaagttgac acttcattta accctttcgg
     3841 tgaagataga aaaccatcac ggtttgaact ggtagaaaag ttcgaaaaag aacggctcgc
     3901 ctactgggaa aaactttatc cggaaaagaa agagccggtt tcgaggccac aacaaccttt
     3961 tgaaacaggg ttcaggaaag aagaaagaag ggtgctgcag attaagggaa gatatattgt
     4021 atgtccggtg aaatcaggcc tgatgtttat cgaccagcac cgggcacatg aaagaatcct
     4081 ttacgaacgg tttattgaat gtctcgacaa ccgttccgtg ccgggccaga aaacaatgtt
     4141 tccggaaaaa attgaactcg atgcatcagc attttatact ctgaaggaaa tagaagatga
     4201 gctccgtgca attggattcg attttgaatt tggagataat tttaatgtgg aattaaaatg
     4261 ttacccctct cttgaaataa gcgaccgcgg cacagaattg cttaaaacgt tactcgactc
     4321 gttcaaaagc ctgcaatcag atcccacggc tgacctgaga gaaaaagtcg caattgcaat
     4381 ggcacaaaca gctgcaatcc cctatggaaa gacccttact aaagaagaga tggaacagct
     4441 tattgacact ctttttgcct gtaaattgcc aaattattca ccatcgggta agccggtggt
     4501 cagtataatg acgactgaag aactcgacaa gagattcaaa taatagcccc gatttacaat
     4561 cattaatgaa atgattgatt atttttgccc gacagttact ttctaaattt taacgttaca
     4621 gcaaattcat gaacggttac tacagaggct ttttcggaat gccgccggtt gtcaagaaca
     4681 tcatatacat aaatattctg atgctccttg cctactatgc agctgcaagt gtttttgata
     4741 tagacctcaa cagaatactc ggaatatatt tcccaaaatc agatcaattc aggccaatcc
     4801 agatactgac ccatatgttt atgcatggtg gtttctggca tctttttttc aatatgttcg
     4861 cactctacat gttcgggcag gttcttgagc aggtatgggg cccaaggcgc tttctgattt
     4921 attattttgt ttgcggtctt ggagcaatgg caacacacat ggctgttatg gcgattcagt
     4981 actacagagt gcttgcttac attactcccg accagttgca ggaagttatt gacaacggtt
     5041 atgtttattc actccagatg aaagcatatc tcgatcctgc aatgaaagaa ctgcagacca
     5101 tactctatac tcccacagtt ggagcttccg gcgctgtttt cggcgtcctt cttgcttttg
     5161 gagtgctgtt ccctaacacc caacttatgc tgcttttccc cccgatacca atgaaagcaa
     5221 aatatttcgt aatgctatat ggcggactcg agttctacat ggcaattaca cagccaggaa
     5281 gttctgtagc acatgccgca catgtcggag gtatgatttt tggatatata cttattgtaa
     5341 tctggcgtaa aacaacaaaa acactttact gatgagtctg tggtctgaca tcagatcaac
     5401 cttcaggcac ggaacaagcc tgaccagact gattatactg aatattgcag tctttcttgc
     5461 tatctctgtt attgggataa tcggatttct tcttgccaat cctgaaatat ctgattatac
     5521 tatccggctt ttctcagtac cttcatcatt taaatacttt atactaaggc cctggacagt
     5581 aataacttac atgttcaccc ataaagacct gctccatatt gcagtaaaca tgctctggct
     5641 ctactggttt ggcaaaatct tccttgacta ccttgaccag aaaaaacttg tggctgtata
     5701 ccttctggga ggaattactg gagctttgct gtatgtgata tcatacaaca tctttccggc
     5761 attttctgaa tttgcaggaa gctcaatacc cctgctcgga gcaagtgctg cagttatggc
     5821 agttgtggtg gcaatttctg tatatgtacc cgattataca gtttttctgt ttcttctggg
     5881 aagaattaaa ataaaatata ttgcccttgt cattttcatt ctgacttcaa tagttgattt
     5941 ccaggcaaat tcaggaggaa aacttgccca tataggaggt gccatttttg gttatatcta
     6001 taccgtatat taccggaaag ggaaagacct gggcaaatgg ctcaacagaa ttattgattt
     6061 ttttgttact tttctaaagc cacgtaaaaa acttaaagta acatataaaa aaagggtgac
     6121 ggatgattat gaatataacc ggattaaagc agaacgtcag gcaaggataa atgaaatact
     6181 cgataaaatt tctaaaggcg gatacgacag tctgacgcgg gaagaaaaag attttctttt
     6241 cagagaaagc cagaaaaaac attaaccttg gtaaagagca aaacttcttt gagatttcct
     6301 ggtgtaagga ggctgattca taatattttt ctgagtctga atatattttt tgccctttct
     6361 ctgatatttt cttatctggc ggtgcacata aatccaggtg atttttccat tccagcattt
     6421 ttcggtctag cttatcctta ccttttgctt ataaacatag tttttcttgt tatatgggct
     6481 gccctgcttc gttgggaggc gcttatatct tttgcggtaa ttgttgcagg gtattcacac
     6541 ttttcaaatt acataaggat ttttaaacct cataacaccg aaaatggcaa catcctcatt
     6601 atgtgttaca acctgcgact aatgaattat tatgaggacc cacaggaaag agagactgaa
     6661 aaaaagatac ttgcattgat taaatcgaag caacccgata ttctttgcct tcaggaactt
     6721 tttatcaatg gcgatgccag ccagaaagac aacgagatta aaaaagctct tggaggtggg
     6781 tattattccc atacaaaact tctcggaaag ggtaaaaata aattttacgg cattgcaacc
     6841 tatacaaaat accggattgc tggaacaggg gaaatcattc atcccggttc atcaagcctt
     6901 tcaatatata ctgatgtgat aattcagggc gacaccttca gagttttcaa taatcatctc
     6961 cagtcgttca ggttgaaaaa aacagaaaaa tcatttcttg aagagttgct tgaagctgaa
     7021 aacagagaag cggtgaatga gattatgtca ttatcggcca gcctgaaaaa aggattcatt
     7081 cagagagcgc ttcaggcaca ggtggtcaaa gatcacataa accggtcgcc ttatccggta
     7141 attgttgccg gtgactttaa cgacaccccc gtgtcgtact catacaggaa aatacgcaag
     7201 gggttaaatg attctttcgt aacatcaggt tacggggccg gattcactta cagaggcaat
     7261 tatccgccaa acaggataga ttacatcctg tatgataatt ctcttgtaaa taccgatttt
     7321 gaaatcctgc gtgtgaaata ttccgaccat taccctgtga tggcatggtt caggaaagct
     7381 gattaaaagg cagttcgctc agttcaacat ttccacccga aagaattata cctgtctttt
     7441 tccctctgaa aacatccgga ttttccatta ctactgcaag agtcacggca gaggaaggct
     7501 caactattat tttcatcctc tcccatatca gcctcattgc ctcaattatt gattcttccg
     7561 atactgtaaa aatattgtct acattcttac ggattatagt aaatgtaagt tcgctgaggg
     7621 aggttagaag cccgtcggca atggtttttg gagaaacaga aggatgcagt attccggttg
     7681 taaacgaacg gtatgcatcg tcggcattac ggggttctgc cccaaaaacc ttaatattac
     7741 tcttcaatcc ctttgctgca gttgaggttc cgctaagcaa ccctcctccg cctacgggtg
     7801 cgatcaatat atccagctct cctgcctctt caagcagttc aacagcagct gtaccctgac
     7861 cgcaaataac gttgaaatta tcataaggat ggaccagcct ggcacctgtt ttttccatca
     7921 gtataccagt ggcctcttcc cttgccttct gagtgggctc gcaaaaagtt atctcagcac
     7981 catagtctct cactgcattt atttttacag aaggtgctgt gcggggcata accacatatg
     8041 ccttcatccc cgccatcttt gcagcaaggg caagtgcagc ggcatgatta cctgatgaat
     8101 gtgttatgac ctccctgact tcttcctctt taagcagtag tacggcgttc atggcacccc
     8161 tgaacttgaa agcacctact ttttgaaagt tctcgcattt gaaaaatatt tctgcacccg
     8221 atattctgtt tatctgcgtt gaagtcatta ccggtgtgcg gtgaacatat gactttatgc
     8281 gccggtgcgc ctctttgata ttttcaagcg aaggaagtat cattatctca gtttaagaca
     8341 attcaaatat aaaaaaaaga gtgccggaaa agaacatgtc ttttccggca ctctcatata
     8401 attttttcta caaaagttta aatgctaccg tcagtcctgt cattacaacg gacaccatcg
     8461 acataagttt tataagtatg ttgagtgaag gacctgatgt atctttgaac gggtcaccta
     8521 ctgtatcgcc aacaactgca gctttgtgcg gatcactctt ctttccgccg tagttgcctt
     8581 tctcgatata tttcttggcg ttgtcccatg caccacctgc gttgttgagc atacatgcaa
     8641 gtgtgaatcc tgcagttagt ccgccagcaa gcagccctac aacacccgga acaccaaaaa
     8701 ttacacctat aagaaccgga acaattattg ccatgagtgc aggtgcgagc atctccctct
     8761 gggctccgcg agttgatatc tcaacacact ttgcataatc gggtttccct gttccttcca
     8821 ttatacccgg tatttcacgg aactgccgcc tcacttcatc aaccattctt cctgcagcac
     8881 gtccgacagc tttcatcgac attgaacaga agacaaaagc catcattgcc cctataaaaa
     8941 gtcctccaag aagtatggga ttaaacagtg aaatgttata tgctgcaacg aaatcaccaa
     9001 tatgtgcctg cgttacctga acggccctct gaccttcctg tattacagcc ggtgcgtggc
     9061 tcttatagaa aagtatgtct cccacctgct tgtatccctc aggtgcttta tctgcaagtt
     9121 tacccagcca catctttacc tcttctatat atgcagcaag aagtgccatg gctgtcaggg
     9181 ctgccgagcc tattgcaaat cctttaccgg tagctgctgt ggtattgcca agtgcatcaa
     9241 gggcatcggt tctttcccta acttctttag gcaggtgtgc catctctgcg tttccaccgg
     9301 cattatcagc tatagggccg aatgcatctg ttgcaagagt aattccaaga gtcgacagca
     9361 tgcctacagc agcaaagcct atcccgtaaa caccctgagc aaaattatgg aaaccacccg
     9421 caaacccgaa agcagcaact atacctgcta ctattgtaac aacaggtacc catgtcgaca
     9481 tcataccaac agccatacct tctataatga ctgttgcagg tccctgctgc gactgttttg
     9541 caatgccacg ggttggtttg tattcatccg atgtaaaata ttctgttgcc tgtccaataa
     9601 ttacgcctgc tattagacct gctatgactg agccgaatac accccaggtg atccagccgg
     9661 tgaaagccat taccgctagc accacgagta taagtgccga gctgccaccc gttcccagaa
     9721 gcagcgcacg cagaaggtta cgctgggtag cactctcctt tgcacgcacc atgtatactc
     9781 ccatgataga aagcacaaca cctacagccg atacaatcat tggagcgata atagctttga
     9841 tctggttttc ggcatctagc gaaggaagtg ctgcaccgag agctgctgtt gcaagaatag
     9901 aacctgcata agactcataa aggtcagctc ccatacctgc aacatcgcca acgttatcac
     9961 ctacattatc ggcaatagtt gcggggttac gcgggtcatc ttcgggtatt cctgcttcta
    10021 ccttaccaac aaggtcggca cctacatcgg ctgcctttgt aaatatgcca cctcccacac
    10081 gtgcaaagag cgcctgagtt gaagcaccca taccaaatgt cagcatggtg gctgtaatct
    10141 caataagctt ctgatgttca gtagttccag gtgcgacaaa agtcaaaccc agaaatttca
    10201 gcccgtgttc catattatca ggtgtaaaca ccgcatagtt aaggatcacg aaccacagtg
    10261 ctatatccag aaggccgaaa cctactacta ccaagcccat cacagcacca ctgcgcaatg
    10321 ctacctgaag acctttattc agggaagtcg acgcagccca tgcagtcctc gaagatgcat
    10381 atgttgctgt acgcatccct aaataaccgc aaagaccaga gaaaaaacct cccgtaagaa
    10441 aagctacagg tacaaatgga ttctgtacac caaaatatgc cagtacaaaa agcagtacta
    10501 ccagaatgag gaaaaccttt gtaacaaccg tatattgacg cttaagataa gccatcgcgc
    10561 cttcacggac atactgggca atttccttca ttctgtcagt cccttcttcc ctgacaatca
    10621 tacttctgta aaaaatccag gcaaaaagta atgcagtaag tgctgcaaca ggtacaatcc
    10681 agaaaatact gctaaccata agttttgatt ttagttttta ttaaaggtta ttctaacaaa
    10741 ttcttaaaaa taattgctgc aaaactaatc agtttttttg ttttgcaaga attttagaaa
    10801 aaattaactg ttaagttctt ttttctgcaa tagaatcaaa ctttttgcat aactcttttt
    10861 ttaaaaaggt atttaatact gccgtcaaaa ttaactttaa tattttcgat tgaataactg
    10921 ttaaataact attgaaaatt ttgttataca ttcgtccccc gataaataaa agaatttaaa
    10981 acaatgaaac cggaaagaaa agttagaata ggtatagcag gttacgggaa tctcgggagg
    11041 ggagtcgagt atgctgtcaa acagaatccc gacatggagt tgattggtgt tttcacaaga
    11101 cgtccacctg aaaaacttca ttgcagttta ccggtatttc atatatcgga tatgaaaaac
    11161 tttaaggata aaattgatgt tatgatactt tgctggggat cagcaaccga tcttcctgag
    11221 cagggtcctg cggcagctga aatttttaat accgtagaca gtttcgacac ccatgcaaaa
    11281 attccagaat atttcataaa aatcgatgag tgtggaagaa aaaacaacac cctgtctctt
    11341 atttcatccg gttgggatcc aggcctgttc tcactggcac gtttactggg tcattgcgtt
    11401 ctgccagaag gcaaagatta tactttctgg ggtaaaggtg taagccaggg ccattccgat
    11461 gcaattagaa gagtgcccgg tgtaagatac ggaatccagt acactgtccc tgtacaggat
    11521 gtggttgaaa agatcagaaa tggagaagaa cccgaactta aatcatccga catgcacctc
    11581 aggatatgtt acgttgtgcc ggaaaaaggt gccgacctta aaaaaattga agaaactatc
    11641 ataaacatgc ctcattattt taaagaatat aaaacgactg ttaattttat atccgaagaa
    11701 gaattcaaaa aaaatcatac cggaatgccc cacggtggaa cagttatccg aagtggtaaa
    11761 agcgcaacag gattaaaaca cagaattgaa tttactttaa atcttgaaag taatcctgaa
    11821 ttcactgcaa gtgttctggt tgcctatgca agggcaattt tccgcatggc aaaagaagga
    11881 aaaaccggag ccctcacagt atttgatatt ccttttgcct acctgtcacc ggaacctgat
    11941 gaaaaactga gagaaaagct tctgtaaacc gtaaaaattt aaattactta cacaacaaaa
    12001 tttataatca tacagttttt tcaaaaaacc tactatggtt aaaatatcgg cagttgttat
    12061 tgcttttaat gaggaagaat ttattggcag gtgcattgaa tctcttaaag aggtcgctga
    12121 cgaaataatt gtagtcgact cttattcaac cgactcaacg cctgatattt gcaggtcgtt
    12181 gaacgtaaaa cttgtgcagc atcctttcga aggatatgct gaacagaaga attttgccac
    12241 atcccttgca acctatcctg tggttctgtc gctcgatgcc gatgaagcgc tcagtgaaga
    12301 gttaaaaagt tctatcttaa aagttaagga agatttcaga tacgatgggt actattttaa
    12361 ccgccgtaat aattactgcg gtaaatggat gtcgcattca aggctttacc ccgacnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncattt tcactttgcc tggaactttt
    12541 tcagatcctt tattctgagc agaggtttcc tagatggtta ctacggatat acttactgta
    12601 tgataactgc atttggcagt ttccttaaat atgccaagct aagaaggtta tggcttctaa
    12661 aaaagcaatc ggacagcagg aaaaacaatg aaaaaggatc ttgatatttc acttgatgtt
    12721 ctcaggaagg gaggtgtaat tctttaccct accgatactg tatggggtct gggatgcgat
    12781 gccacatgtg ctgaagcagt tgaaaaaata ttcgctataa aacagagacg cgacagcaaa
    12841 agcctgatta ttcttgtaaa tagccatgag atgctggaag aatatgttca ggaaatacca
    12901 ccgatggcag aagaaataac aaagatagcc gacaaaccaa ttaccatagt gtatccccgc
    12961 agccgtaacc ttgctccggg tgtttcagcc gaagatggaa gcgttggaat aagaatcaca
    13021 aaagacaaat tctgttcggc tctgatagaa aaactgggca aaccattggt ttccacatct
    13081 gcaaacagaa gcggcgaacc tgcacccggc aacttcagtg aaatacctga ggaaataata
    13141 aaggctgctg attatacagt tacaatcaga agggaagaaa aacgaaaagc aaagccatct
    13201 ccagtaatta gagtcgagat gaacggggaa ataaaaattc tgagaagata aaaccttgcg
    13261 agtgcccggt tatttattaa tatctgacag cccctcaggt aaattcatgt agatgatctt
    13321 ctgtttttta tttatcgcga caatcagatc ctcatgaaaa ggaataaata attctgatcc
    13381 gctattttca tcctttacct tcagcagcaa atttccagga ttatctatga ctgcaattat
    13441 attccctgat attccggaac cttcagatat tattctgtaa ttcagaaact catcccaata
    13501 aaacactttt ttctctttcc gcgacccgga gactttaaca taaacattgc atccggtaaa
    13561 ttccttaact tttcctgcac ttccgtatcc ttcaaatttc aggaaaaggg ttttattatt
    13621 ctgcagttcg cacgtttcaa cacaaaaagg gacctttttc ccttctattt caaggaaaac
    13681 aaagtccggt ttactaaatg cttttgataa gggtttatca gtgcttaccg caactacacc
    13741 actgataccg tgaagtttaa gaattttccc aagcaggata tattcctcca tccgggatgt
    13801 aatataaggc ttcccggaga ttaccggggt tttttctact gttccgaaga agtttcagta
    13861 tcagaggcgg atgactcttc agttgatggt tcagcttcag cagcactggc tgccatttct
    13921 gcatttttct cagcattttt ctttgccagt tctgcagccc tcttttcatt tatcttcttt
    13981 tcttcttcga tgcgtttcct cagctcctca tctgctgctt tttcaagttt ctgaatcttc
    14041 tcctgaatct ttgcctcttt ctgcttcagc cattcattga aacgcctttc agcttcagct
    14101 tcgtcaaatg cacctttctt cacgccctca agcaggtgtt tcttaagaag aacaccttta
    14161 tgagataaaa ttgccctgca agtctccgta ggttgagcgc ctttctgcag ccaatccagc
    14221 gacttgtcga aatcaagctc aacagtggcc ggatcagtac gcggatcata aattccgagt
    14281 ttttcaataa atctgccatc ccgtggcgac ctgctatctg ctaccactat gtggtaatag
    14341 gccagcttct tgcggccctt cctagctaat ctgattctaa ctgccattca aatacgctta
    14401 gttatttaac ctacttttta aaattgtggc tgcaaagata aatatttatt acaaattatt
    14461 ttcaaaaaat tatcgaaaac cgtgctgtca acaaatttat ttcttttata cgccggcaaa
    14521 atcacctgta tccttcattc tgctgcgact gaagagcggg attgttaaga atctccctcg
    14581 ccggaatcgg aaaaaccagt tttggatttt tataattcca tggccctata ttctgtttca
    14641 gccttttgat atcatgaatt ctatgcccct caaaagccag ttcacgcctt cgctcaagaa
    14701 ttagctcatc cagactcacc gagccagcag caggcaaacc tgccctggtg tgcactgcat
    14761 tataatcatc aaccggactt gcaccaacat acggaggtcc caacctcaga ttgcattccg
    14821 cccttatcag aaacatctcg gccagacgta tgagatttac acaaccatac aaattgttcc
    14881 acttcccgca tcttaaggcc ccgtccccgg tataaaacaa ctcggctctt ttatcctctt
    14941 tagggtaaat attaagatgt ccgtcaagaa tttcaataaa accgttacct ccgaacgaag
    15001 atgttgagaa agaaactgtc attgcattgt actctgtggg atatgagatt tttgccacaa
    15061 aaatatcttc ctttgttggt tcatactggt tgaatatgct ggcataaaca ggaagaaggt
    15121 tgtattttcc cgatgaaatg acagcatcgg cagccttcct ggcattgtcg taatcacctt
    15181 tctgaagata aactcgtgca agaagtgcat tggctgctcc acgtgtggca aatatccagt
    15241 tgtcatcggg cagtctttca gctgctgcca ccaggtcttt aattatctgg ttatatacct
    15301 cctcaaccgt attgcgtccg gtgtaaacct catcgctgat ttcacgtatc ggtttcagga
    15361 tgagcggtac cccatactgg ttatttaaag cacctggttc atagggcagg gcaaaaaacc
    15421 tcacaaggtc aaaatagatc aaagctctaa gaaaaagagc ttctccttcc acccagccac
    15481 gatcagcctt tttcaccaca tcgagggcac tgagaacatt gttaacagta ttgattaccc
    15541 tgtaactttc gcgccattgt gtaaaaacct cactattatc tgctgaaatt gtttttgtaa
    15601 aaaattcgaa aggttcataa taggtctctt catttaccca taatatttca ccgttacctg
    15661 caagaagttc ggcattcctg agaatattgc ccccgtatat acctggctca ttgaaaagtg
    15721 aataggcccc attaagaatg cttttgacat ttgcatccgt cgatagagca acttcctcac
    15781 tgacactctg cgaaggttca ggttcaagca gccagtcaca acctgttaac agcataaaaa
    15841 gcgttaaaac tgttgtcaga ataatacttc tattcataaa ttcctccttt aacattcaga
    15901 atccaagtgt tattccaaaa actatggttc gcggctgagg tgccgaataa aaatcaaatc
    15961 ccgaaaaaat gttgtttacc ataaaatctg cagaaacttc cggatcccac ccttcgtaga
    16021 ggctgaaggt gaaaaggttc ctcccctgaa taaataactg catttttcta atattcaatt
    16081 tattagttac tactgagggg acgtcatagc ttaaagagac ctcccgcagt ttcaggtatg
    16141 aaccatccga cagatacctg ctgctacggc tctgattgcc attatcccag agcagccttg
    16201 cctgaggcac atcggtaata tcgcccttcg acttccagct gcgtaactgc gacttaagct
    16261 gattgtcgaa ccagacaccg tttgaagcca tccacctgtc gccggcaaga tgtattttgt
    16321 tgcctgcaac tccctgaaaa gtgaaaatca attctaaccc tttgtaacac aaggtattag
    16381 taatagcccc catgaaatca ggattgggat tgccgagaat aataaaattc gcactattaa
    16441 aattactggt agttttatcc gggtttataa tgttcccgtt tgcatccttt tcgttgatat
    16501 accacagggc atcgccatta ttgtagtcaa cacctgcata ttcagcacca tagaaagacc
    16561 caatgggata accggtcatg gcaacattca tataactttc accaccgaaa tcaagaatat
    16621 tttgtttgcc cagacttaat accttattct tgttaagcga aaaattcagg ctggttttcc
    16681 attcaaacaa tcctgtgata ttaaccgtgt ttaaaacaaa ttcaaggcct ttgttttgaa
    16741 ttgctccaga attctgaatc cgggtcaggt aacccgttgt tgaaggcact tcttccccaa
    16801 ggagaatatt ggttgcttta cgctggtata tatcaatttc gcccgaaatc cggtctttaa
    16861 ggaatccaaa ttcgacacct gtattaatct cactcaggga ctcccactta aggtcagggt
    16921 tggctatctg tccggggacc aggcctggca catcaccata acttcctgaa tcatacaatc
    16981 caaggtgcca gtaatttcca aaacctgcat ttccagccaa tccgtatcct gccttaattt
    17041 tcaaaaaact caggaaaggc acactctgca taaaattttc tccggtcata atccagccgg
    17101 cagatattcc cgggaaaaag ccaaacttat tgttgcttcc gaaacgggaa gaaccttcaa
    17161 tcctgccgga tagtgaaaga agatacttgc cgtcatagtt agtatttact cttaaaaacc
    17221 cggcgagaaa gccataacgc gacaatgttg atgacccgcc tgagattatg ccggcgcttg
    17281 ctattgtttt gagagcatca gaagcaaaac gctctccttc ggtgtacata gtctcaactg
    17341 aagtatgttg aaactccgtt cccagaagag tgtttaaact gatattgctg agttttctgt
    17401 taaataaaag aagtgacctt ccggttatat tctggttctg tccgtgatta gcaaatccat
    17461 agccgctcct gtccatacct tccatggttt tttctccata acgtgcattt tccttattac
    17521 catagaggtc aaagccaagt tcatttttcc atgtaagtcc ttcaataata ttaaaattta
    17581 tatatccact tgtaagtgag cggtattcgg taattttttt cgatgcatat tcagcatcaa
    17641 gaagtgcatt gtagtatgag gtaaccggga aggtgctcag ttcaccgtct gcgtcgcgtg
    17701 gaggtgttac cggtgcctga gctacaagct gcaaaggtga agataaataa ttgtcggacg
    17761 atacctggtc aatggcagcc ctgttaattc ttaaagaaaa accaaggtca atacgtctgc
    17821 ttactttatt gtcgatcctc aggcgcgctg atattttttc aattccgttt gatacaagaa
    17881 taccatcatt gatgagaact gagctgcctg cactgtactg aagtttatcg tttcctccct
    17941 gtgctgaaag gtctgccgac cagattctcc ctttcctgaa agcaaggctc tgccagtcag
    18001 tactaac
//
LOCUS       DAOSNB010000016        17787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3204_length_17787_cov_4.913264, whole genome shotgun sequence.
ACCESSION   DAOSNB010000016 DAOSNB010000000
VERSION     DAOSNB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3204_length_17787_cov_4.913264"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PLG42_01545"
     CDS             <1..1172
                     /locus_tag="PLG42_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOU29925.1"
                     /translation="DYENGKAHEQDIEDIGDFIVVNSKFISSDGEVKKYTNTAFCPAQ
                     KGDILMVLSDVPNGKAIAKCFLVEENNRYTVNQRIGRIIPQSLNSKILYYLLNRNPYL
                     LAFDDGVKQTNLRKEDVLNCPLLIPKNPKEQQKIASCLSSLDEVIAGERQRLELLKAH
                     KKGLLQNLFPREGETVPKLRFPEFKDSGEWEVTALSELGYLINGLTYSPEDVREKGLL
                     VLRSSNIQDGLIDLKDCVYVRENIKGVNLIKPRDILVCVRNGSKDLIGKNAMIPEGIP
                     LATHGAFMTIFRANNPEFVFQLFQTDYYDKQVRADLGATINSINGKNFIKYKFPVPEN
                     PKEQQKIADCLSSLDDVIQAQQQKIELLEQHKKGLLQGLFPNLKQLDNERCNYDI"
     gene            1147..2292
                     /locus_tag="PLG42_01550"
     CDS             1147..2292
                     /locus_tag="PLG42_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU29926.1"
                     /translation="MSGAIMTYNDLASLVTHLRSLNKKHILLFAYNSTGKTRLSFEFK
                     EAGKVYDDDHNVISRDTLYFNAFTEDLFLWDNDLDDDTNRRLLINKNSAFISGMRALD
                     MDNKIRPLLHRYSNFNFIIDYEYKNKEGKELWAVNFIREELINGTLQNVEYVKISRSE
                     ENLFIWCFFLAIAQLAIDRHENYNWVKYIYIDDPVSSLDDNNVIAIAHHLAMLLKSEN
                     ANENGEIKTIISTHHALFFNVLCNEFNRGAKYFLCKIAEGYALKDTTDSPFLYHVSLI
                     QELKKAIDTGKLYTYHFNILRTILEKAANFHGFNGFSDCLEVDEDDDERTLHARMVNV
                     LNHGSYSLYDPKEMLEENKKYFIQIFNNYMKNYKFNKKLLENINETI"
     gene            2294..3904
                     /locus_tag="PLG42_01555"
     CDS             2294..3904
                     /locus_tag="PLG42_01555"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOU29927.1"
                     /translation="MTTNNHTELGKTLWKIADDLRGSMNADDFRDYMLSFLFLRYLSD
                     NYEEAARKELGKDYPSQNGNGGRTPLSVWYEQNPGDVAEFEKQMRRKIHYVIEPQYLW
                     NNIAYLAKTQSRDLLHTLQKGFKYIENQSFESTFQGLFSEINLDSDKLGKSYEERNKK
                     LCTIIQKIAEGINQFSTDKDILGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSEIV
                     ILDSQDPAQGKKKKIERVLDFACGSGSLLLNVRRKIIEANGTIGKLYGQEKNITTYNL
                     ARMNMLLHGVKDTEFEIHHGDTLLNDWETLKEINPAKKLEFDAVVANPPFSYRWEPTE
                     EMAQDFRFKDYGLAPKSAADFAFLLHGFHFLGKEGTMAIILPHGVLFRGGAEEKIRRK
                     LLVDGNIDTVIGLPANLFFSTGIPVCILVLKKCKKFDDVLFINAAEHYEKGKRQNTLL
                     PEHIRKIVETYQFRKEEDHYSRRVSMAEIEKNDFNLNISRYVNTAQEEEQIDLTDVSN
                     KLTEIEQQIAKARETHNQYLKELGLPPI"
     gene            5341..5805
                     /locus_tag="PLG42_01560"
     CDS             5341..5805
                     /locus_tag="PLG42_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU29928.1"
                     /translation="MTDKTVVQHLDIQKYLGTWYEIARYDHKFERGLVGVTATYSMRD
                     DGMIKVINRGFKGTLDGKPSEAVGKAKIPDPSVPSRLKVSFFLFFYGDYYVLELDQDY
                     RWAVVGSSSDNYLWILSRTPQMDESLYRQILDKLVKRGYNVEKLIKVPQRNN"
     gene            5807..6211
                     /locus_tag="PLG42_01565"
     CDS             5807..6211
                     /locus_tag="PLG42_01565"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOU29929.1"
                     /translation="MARLLFQFLIFIFLPLQAQYSVVIKTEKLRNNNGTIFWELTDEH
                     QARIAGGIQNIENNTCIISIKNIQKGKYIFKYFHDENCNNKLDKNCLGIPTEGFGFSN
                     NAKGSFGPPETGKTIFKIENDTLLIYHPTYYL"
     gene            6228..6704
                     /locus_tag="PLG42_01570"
     CDS             6228..6704
                     /locus_tag="PLG42_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU29930.1"
                     /translation="MEKQVNPVEQDLAEKRIINIETIKELWSKTYNTEGRPDWSHILP
                     FYDEDIIFRDSIQELHGFQDFKEMTERLTKRSKDLKIKIVNAIMSGNTAFIEWEMTLS
                     FKKYPSSILYGASRLTLSEEGKIKEQRDYYDLWGDIFDNIPRFGKIYRKFVIKKFG"
     gene            6710..7528
                     /locus_tag="PLG42_01575"
     CDS             6710..7528
                     /locus_tag="PLG42_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU29931.1"
                     /translation="MALIDKRTLRYIKEYRWSNVFEMIRNNRRSPDICEEDFTGKLVV
                     VSGATSGIGYSTVRKYASKGANILCINRNPEKSEKLKDEIEKEFNVKCDYIIADLSSI
                     EQTHNASSKLLELTIPIDVLIHNAGVYFTRKQVTADGIEYLFMVHYLSSFIINYILKE
                     KLKAQGNSRIILVSSEGYRFAAWGLRLDDLNWEKRRYSGLKSYGSAKLAQLLTMLLFD
                     DYFRVSGVTINAMHPGAVKTDSGQENGKFYKWFKRNILDKALKPPDISADRIFK"
     gene            7595..9130
                     /locus_tag="PLG42_01580"
     CDS             7595..9130
                     /locus_tag="PLG42_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOU29932.1"
                     /translation="MDKNLFRSGKIVPVFLLAAAVFSISILTVNAQISSGKVKPRIVI
                     TTDPELDDSNSLIRFLLYSTDVEIEGLVYASSQFHWKGDGKGTRWYVPGREYDRFGMN
                     ECPCESWRWSENSRHIHDVVEAYEKVYPNLIKHNPDYPSPEYLKSKIRYGNIEFDGDI
                     SKDSPGSDLIKSLILDDRPGKLFLAAWGGHSTIARALKSIQEQYEYTTEWEALKKKIS
                     RKVVLLPSGDQDDTYAKYIKPNWPDIEYRQFRGGPNYGYGAQIKASPEDAAYMTPEWM
                     KENITSRGPLGELYRVWGDGKQMVKGDRLDYFGLSGYTDEQLRKMGYIVWMPVQKKGS
                     WLGEGDNHTFMNMLDNGLRAWEKGYYGGWGGRVIEAREASPGLSVSKMDYPDFFPQAQ
                     RDFANRLKWSVTDKYENANHEPVVTVEGPLEFLASPGQRVAIHGRVSDPDGNKVAVKW
                     WQFNVGTYPGKVEISHPDSLKTYVTIPKDAAGGQTIHIILEATDNGEPPLTRYQRIII
                     TIR"
     gene            9166..10380
                     /locus_tag="PLG42_01585"
     CDS             9166..10380
                     /locus_tag="PLG42_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HOU29933.1"
                     /translation="MKNVIINLNAMVLLIIFSGYLYSFRKEKVQPQQPVSGYYVAAYV
                     WPSCHDDPLGRAKLWPEGIGEWEVIKKGTPRFEGHYQPRQPLWGYEPDNDPRVVEKWI
                     DAATDHGVNVFVYDWYWYEGGPFLESALNDGFLKARNNEKMKFYIMWAYHDVRYNYWN
                     VHLYRDNNNILFNAKVDWENFRIIVERVINNYFKRPNYFKINGEPVFSIFSVNKLIES
                     FGGSVEETRKALDYFRDEVKKAGFPGLHLQWNQGGGSVMTQEAAERFAARIKELGFDS
                     FAMYNMGGFNEDYLVYGTNSLKIREQLDAILDIPVFPCVSIGWDDTPRFPGKGIKDVV
                     HYHNTPESFAALLSKAKRYADTHPEQPKLITINAWNEWVEGSYLLPDMLNGFGYLEAV
                     KEVFIDGRYDRY"
     gene            10757..11224
                     /locus_tag="PLG42_01590"
     CDS             10757..11224
                     /locus_tag="PLG42_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOU29934.1"
                     /translation="MADITAPKILMDHGLKVTPQRTLILEALISLDEHPSYEIIMKFM
                     RFRYPNISLATIYNTLKLFKEKGIIKQIAAGDEEIRFDPVQKKHHHLYYKDTDKLEDF
                     EDKELDRILKNYFNKKQIPGFKIEDISIMLTGHSEKTQTPEKSKQKTQKKIIK"
     gene            11324..11824
                     /locus_tag="PLG42_01595"
     CDS             11324..11824
                     /locus_tag="PLG42_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOU29935.1"
                     /translation="MAEIGRSIVKMDVDKLINLLNKALSDEWLAYYQYWIGAKVVKGP
                     MKDAVISELQLHATEELTHADLLATRIVQLGGTPVLSPQEWLKITNCGYDAPEDPFVG
                     VILDQNIKGEQCAISTYNKLLDITKDADPITYNIILQIITDEVEHEEDLAALKEDLEL
                     MLAKHK"
     gene            11843..12553
                     /locus_tag="PLG42_01600"
     CDS             11843..12553
                     /locus_tag="PLG42_01600"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOU29936.1"
                     /translation="MCEEFAVSMPRIGDKAPEFKAVTTQGEINFPADFKGSWVIFFSH
                     PADFTPVCTSEFMTFATMEKKFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVTFPLIEDITMEISKKYGMIQPGESSTKAVRAVFFIDPNGIIRTIIYYPLSLGRNF
                     EELYRVLIGLQTADAFSVALPADWQPGDDVIVPPAGSCGVAKERMEGKKEDIKCYDWF
                     FCTKKLPKESIFKKILNK"
     gene            12764..13156
                     /locus_tag="PLG42_01605"
     CDS             12764..13156
                     /locus_tag="PLG42_01605"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOU29937.1"
                     /translation="MIEIYFEGGKVVTANVHGHTIRTDQPVESGGNNSAPAPFDLFLA
                     SIGTCAGIYVKSFCDRRGIPSDGIKIIQTMEVDQEKRLPSLFKLEIQLPPDFPEKYTE
                     AVINAADLCLVKKTIYNQPKFEITATKK"
     gene            13161..13550
                     /locus_tag="PLG42_01610"
     CDS             13161..13550
                     /locus_tag="PLG42_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOU29938.1"
                     /translation="MKIAVPVTSDNKLDSHFGHCRHFKIFSVKNENEIECIKKISPEN
                     GHGCKSGIVSLLAAEGVEVLLAGGIGEGAVNNLNSAGIKVIRGCAGEPDDLVRLYIEG
                     KIDDSGESCRHPRHHQHEGQQGTCHHS"
     gene            13596..15392
                     /locus_tag="PLG42_01615"
     CDS             13596..15392
                     /locus_tag="PLG42_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOU29939.1"
                     /translation="MTNIIFTPVLTDVGHKIMEHIKPGELKQVWLKASVVGSIWASFE
                     IILGSFLHNLKIPISGTILSFIGVWILISFQLVWKDKGLVWRAGLICALMKSISPSAI
                     ILGPMIGIFTESLIIEITTRLLGRNFLAYIIGGALAVLSTLVQKIINLIILYGLDLIK
                     ILDNLYRFAVKQIGMEQLNPVYVIAAITALYLLAGMAGATGGFLTGKRYLKLKRLQET
                     EEKITLKPGTELFEKTSEDKYSILFLLLNLFAIIGILLLLNYNLLIIAGIVAVIYSTF
                     CILTYKNSLRRLKKPGFWISFFIITFSAAFIWNWFSDGIFFTTEGFLVGLKMNARATV
                     MIIGFASISAELKNPVIKSMLYNRGFASLYQSLNLSFSALPYLISTLTIKEEQGKFPG
                     KKSLHGILSRADNLFNVFENEYRKRPSIVIITGEVHAGKTTYAMKIINALRERNIQAC
                     GFLALAVNENGERKGFNLLDLETGEEKELCSTSETPGSLKSGRFYFRRETLEKGLKIL
                     TNIPHTCPCVVIDEIGPLELAGGGWSMAIEDLCRKNVSPQIWVVRKPILHQLIRRWNT
                     GDIYIFDIQTDSIKDAVEKVLELSLKQKPAGT"
     gene            complement(15395..15607)
                     /locus_tag="PLG42_01620"
     CDS             complement(15395..15607)
                     /locus_tag="PLG42_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOU29940.1"
                     /translation="MKKNTGKLDRIIRLIIALLIGILYFTNVISGTLGIVLLVFAVIL
                     LITSITGFCGIYTLFGINTCKTRVEK"
     gene            15829..17298
                     /locus_tag="PLG42_01625"
     CDS             15829..17298
                     /locus_tag="PLG42_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU29941.1"
                     /translation="MKKIHLSWLVILIISTIFFQECRNKSEYNETIKRIAALVEEGAL
                     EKAAMIADSLKNATRDDDLRWKADSLNEITKRIRLDFSVTQNGIDTILGKLVQGYSES
                     DRKIWEENGWLEYRIIDGEKRYFNRAAKNLILLRNFYQNRQYRDSLTARDPRIVFRKN
                     HTADIIRVSGKSPVPVMPIQIEVTYTITVKPDAVPAGSTIRCWMPYPKENNKRQTEVY
                     LLGISNENYFLLAPDSVVHKTVYMEEKARRGIPTVFQIAFSYFSYGQYFDPENMKILP
                     YDKNSEIYRKYTREQPPHICFTDRVKHLADSLAGNESNPYEIVKRFYYWFNNNIPWAG
                     ALEYSIIPNIPEYTLKNRRGDCGMQTFLLMSMLRYKGIPVKWQSGWMVPPQGENLHDW
                     CEVYYEGTGWVPLDISYSLQYSNNQKLKEFYITGIDSYRLIINDDIGGKLYPEKKYLR
                     SEPFDFQRGEVEWEGGNLYFDQWDYDIQVKIKPLPGTRI"
     gene            17332..>17787
                     /locus_tag="PLG42_01630"
     CDS             17332..>17787
                     /locus_tag="PLG42_01630"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU29942.1"
                     /translation="MNNTKLLRILIFLITALMVVAEGCRFPVGIRLVNQIDSISKKWV
                     PDSREGVFKVEISRKGTILMLKGETDIPEAKADIINLLKSRKTKFIDSLIVLPDTSVI
                     KKPWGLVAVSVCNIRSAPDHSAELTSQALMGTPVKVLKKKNSWYFIQTPD"
BASE COUNT         6019 a         3407 c         3537 g         4824 t
ORIGIN      
        1 ccgattacga aaacggtaaa gcccatgaac aagatattga ggatattggc gactttattg
       61 tagtgaactc aaaatttatt tcttctgatg gtgaagtaaa gaaatatact aatacagctt
      121 tttgcccagc acagaaaggt gatatcttaa tggtgcttag tgatgttccc aatggaaaag
      181 caattgcaaa atgttttttg gttgaagaaa ataatcgcta cacagtgaac caacgaattg
      241 gcagaattat accacagagc ttgaatagta agattttata ttatttacta aacagaaatc
      301 cgtatttatt ggcatttgat gatggtgtaa aacaaaccaa tctgcggaaa gaagatgttt
      361 taaattgtcc tttgcttata cctaaaaacc cgaaggaaca gcaaaaaatc gcctcctgcc
      421 tgtcgtcctt agatgaagtg atagccggag agcggcagcg gctggagctg cttaaagccc
      481 acaaaaaggg tctgctgcaa aatctcttcc cccgggaagg cgaaaccgtg cccaaactcc
      541 gcttccccga gtttaaggat agtggagagt gggaagttac tgcactaagt gaattaggat
      601 atttaatcaa tggacttaca tatagtccag aagatgttcg agagaaaggt ttattagttt
      661 taaggtcgtc aaatattcag gatggactta tagatttaaa agattgcgtt tatgttagag
      721 agaatataaa aggagttaac ctaattaaac ctagagatat tttagtgtgt gttagaaatg
      781 gctcaaaaga tcttattggt aaaaatgcaa tgattcctga aggaattcct ttagcaacac
      841 atggtgcatt catgacgatc ttcagagcaa ataatcctga atttgttttt caattattcc
      901 aaacagacta ttacgacaaa caagttagag cagatttagg agcaacaata aattcaatca
      961 atggaaagaa ctttattaaa tacaaatttc ctgttcctga aaacccaaaa gagcaacaaa
     1021 aaatcgccga ttgtctgtcg tcattagatg atgtaataca ggcacagcag caaaaaatag
     1081 aattattgga acagcacaaa aaagggttgt tgcaggggtt gttccctaat ttaaaacagt
     1141 tagacaatga gcggtgcaat tatgacatat aatgatttag cgtctttggt aacgcattta
     1201 cggtctttga acaagaaaca tattcttctt tttgcttata atagcaccgg caaaacacga
     1261 ctttcatttg aatttaaaga agcgggaaag gtatatgatg atgatcacaa tgtaatttca
     1321 agagatacgc tttactttaa tgcttttaca gaagacttat ttttatggga taatgattta
     1381 gatgatgaca caaatagaag attgctaata aataaaaact ctgcattcat ttcagggatg
     1441 cgggcgctgg atatggataa taaaattcgg cctttattac atcgttattc gaatttcaac
     1501 tttataattg attacgaata caaaaacaaa gaaggaaaag aactttgggc tgtcaatttt
     1561 atcagagaag aattaattaa tggaacttta caaaatgttg aatacgtaaa aatttcaaga
     1621 agtgaagaaa acttatttat atggtgcttt ttcttagcaa tagcacaatt agctattgat
     1681 cggcatgaga actacaattg ggtaaaatat atttatattg acgatccagt atcatcccta
     1741 gatgataata atgtgatagc cattgctcat catttagcaa tgttacttaa atctgaaaat
     1801 gcaaatgaaa atggtgaaat taaaactatt atttccacac atcatgcttt gttttttaat
     1861 gttctttgta atgaatttaa taggggtgca aaatattttt tatgtaaaat agctgagggt
     1921 tacgctttaa aggatacaac tgattctccc ttcctttatc atgtttcttt aattcaagag
     1981 ttgaaaaagg caattgatac agggaaatta tacacttatc acttcaacat tttaagaacc
     2041 atacttgaaa aagcagcaaa ctttcatggg ttcaatggtt tctcggattg tcttgaggta
     2101 gatgaagatg atgatgaaag aactttgcat gcccgtatgg taaatgtctt aaatcatggt
     2161 agttactcac tttatgatcc gaaagaaatg cttgaggaaa acaaaaaata ttttattcaa
     2221 atcttcaata actatatgaa gaactacaaa tttaacaaga agttgcttga aaatataaat
     2281 gaaacaatat aatatgacaa caaacaacca taccgaatta ggcaaaaccc tctggaaaat
     2341 cgcagacgac cttcgcggtt ccatgaacgc agacgatttc cgcgattata tgctttcgtt
     2401 tttatttctg cgctacctga gcgataacta cgaagaagca gcccggaaag aactcgggaa
     2461 agattatcca tcgcagaacg gaaacggggg cagaactcca ttatccgttt ggtatgaaca
     2521 aaaccccggc gatgtggctg aatttgaaaa gcagatgcgg agaaagatac actatgttat
     2581 tgaaccgcaa tacctgtgga acaatatcgc ataccttgct aaaacccaga gcagggactt
     2641 gctgcacacc ttacaaaaag gctttaagta cattgaaaac caatcgttcg aaagcacctt
     2701 tcagggtttg ttttctgaaa tcaatctgga ttctgacaaa ctcggaaaaa gttatgaaga
     2761 gcgcaacaaa aaactttgca ccatcattca gaaaattgcc gagggcatca accagttttc
     2821 caccgataag gatattttag gcgatgccta cgaatatctc attggtcagt ttgcagcagg
     2881 ttcgggcaaa aaagcgggcg aattttacac cccgcaacgc atttccgata ttctttccga
     2941 aattgttatt ctcgacagcc aggaccccgc tcagggcaaa aagaaaaaaa ttgagcgggt
     3001 tttagacttt gcctgcggtt cgggttcatt actcctgaac gttcgcagaa aaataatcga
     3061 agcaaatgga accattggca aactatacgg acaggaaaaa aacatcacca cctacaacct
     3121 tgcccgaatg aacatgctgt tgcatggggt gaaagacacc gaattcgaaa tccatcacgg
     3181 tgataccctg ctcaacgatt gggaaacgtt aaaagaaatc aatccggcca aaaaattaga
     3241 atttgatgcg gtagtagcca atcctccctt tagctaccgc tgggagccca ccgaagaaat
     3301 ggcacaggat tttcggttta aagattacgg tcttgcccct aaatcggcag ccgattttgc
     3361 cttcctgctg catggttttc attttcttgg caaagaggga accatggcga ttatcttgcc
     3421 acacggcgta ttgttcagag gcggagcaga ggaaaaaatc agaagaaaat tgctggtgga
     3481 cggcaacata gacacagtta tcggactgcc tgcaaacctg tttttctcta cgggtattcc
     3541 ggtttgtatt ttggtcttaa agaaatgcaa gaaatttgac gatgttttat tcatcaatgc
     3601 tgcagagcat tacgagaaag gcaaacggca aaatactctg ttacctgaac atatccgcaa
     3661 gattgtagaa acctaccaat tccgaaaaga agaagaccac tactcccgaa gagtttccat
     3721 ggcagaaatt gaaaaaaacg attttaacct gaatatttca aggtatgtga acaccgcaca
     3781 agaggaggaa cagatagatt taacggatgt gagtaataaa ctaaccgaaa tagaacagca
     3841 gatagcaaaa gcaagagaaa cacataatca gtatttaaaa gaattggggc taccgccaat
     3901 ataaccagac gaaatgctaa tatcattttt aagcacattt ataaacatct aacaaagcca
     3961 acgaaagata tatgcgaagc atttacaaac acctataagt tcttcaaaaa aattaaaata
     4021 cgccatgcaa aaccgattag ccaatgtcac ggaaattcaa tactgacaat aatttacaaa
     4081 accggatcat gagcgccatc tgaaagtttg tctcccctgc tcaaatctgc taataggcta
     4141 tctaacggta cttaaagtct acgaaaccta aaagcatcat attagatata ttcttgcatg
     4201 ataaaattcc tgtacccaaa ttcctatctt tgaaaaaact ttaaaataat tatgtatcct
     4261 tctttgatat attaagatga agccacattg gtttaaaaga aaaggtatat tttttattcc
     4321 caaaagtctt ttgggttggg caataatgct aatcgcattc atttatgcaa catatatttt
     4381 tattgaaata gatagcagat ctcattctgt tagtgatact ttaagaccat tctttataca
     4441 tcttttatta attggacttg catatacctt gatagcttta ttaacaagcc ggggaccaac
     4501 agataaaaag taacaatatg actgatatat aaatgctatg aaacctaact ctgatagcca
     4561 tgaaaaactc ctactccttg ttggcttttc taccctcctg aaaacttcct accgtcaaga
     4621 agaaatgaaa atttggaatg aatttctgtt tctggttata aacaataaaa tggccacaga
     4681 gaggatcaaa ccacatgaca aacctggaga caaattcaaa cctgttctgc taaaatataa
     4741 atcccgagtc tttatcagac aatcaggcga ctgtacagct tgaaacgata tatttttact
     4801 ttttaaatat tgcaggtcac cttaaaacaa gaatctaaat cgaggattat aaacaaatct
     4861 ttgagaccat atggcaagac agagtcagaa atgccggatg gaaccttgat attaaatatt
     4921 cacctgacaa taaaataact ttcaatttct agcggtatta ataaaaaatt cccagcttac
     4981 acggactgtt tactcaatat aatcttgtta tactttttgc cccaccttgc agaacttcat
     5041 tacaccttct tgtggttcct caactattac tcctgcaccc gggaaggatt tgatattgtt
     5101 cctgctgcca gcctattgta cgccagggca tgccttccag aatttactct gctcttatgc
     5161 ctgatatatc aattttcttt ttagcggtcg caatagaaat taattatatt ttcttatttt
     5221 caacactgta aatatccagg aattatgaaa acaatcagta aaaaatttaa aatacttagt
     5281 attttaatca gtcttttaat tccaggctca ttttcaatat gttgtaaaac acaggtaact
     5341 atgactgata aaaccgttgt tcaacatctg gatatccaga aataccttgg gacatggtat
     5401 gaaattgccc gttacgacca caaattcgaa aggggactgg ttggagtcac cgcaacctat
     5461 tccatgcgtg atgacggaat gataaaagtg atcaacagag gcttcaaggg cactctcgac
     5521 ggcaaaccgt ctgaagcagt cgggaaagca aaaatccccg acccttctgt tccttccaga
     5581 ttgaaagtct ccttcttcct gttcttttat ggcgactatt atgttctgga actcgaccag
     5641 gattacagat gggctgttgt gggcagcagt tccgacaact acctctggat tctgagccgc
     5701 accccacaga tggacgaatc actttatcgc cagattctcg acaaactggt gaaaagaggg
     5761 tataatgttg agaaactgat caaagtccca caaaggaata attaagatgg ctagactttt
     5821 atttcagttt ctcatattca tcttcctgcc attacaggct cagtattcag ttgttataaa
     5881 aacagaaaag ttgcggaata ataatggaac tatcttctgg gaacttactg acgagcacca
     5941 ggcaagaatc gcaggaggca ttcaaaacat cgaaaataat acatgcatta tttcaatcaa
     6001 gaatatccag aagggaaagt atatctttaa atatttccat gatgaaaact gcaacaataa
     6061 attagataag aactgtctgg gaattccgac agaaggattt ggattttcaa ataatgcaaa
     6121 aggctccttc ggtccgcctg aaaccggaaa aacaattttt aaaatagaga acgacacctt
     6181 attgatttat catccaacct attacctgta attcaaaata ctgtactatg gagaaacaag
     6241 ttaatcctgt cgaacaggat ctggccgaaa aaagaataat aaatattgaa accataaaag
     6301 agctgtggtc aaaaacttac aatacagaag gcaggccaga ctggtcccat atcctgccct
     6361 tctacgacga agatattatt ttcagagact ccatccagga actccatgga tttcaagatt
     6421 tcaaagaaat gactgaacgt ttgacaaaac gttctaaaga cctgaaaatt aaaatcgtaa
     6481 atgcaataat gagcggtaat acagctttca tcgaatggga aatgacactc agcttcaaga
     6541 aatatcccag ttcaatactt tacggagcca gcagacttac gctcagcgaa gaaggcaaga
     6601 ttaaagaaca aagagactat tacgatctat ggggagatat atttgataat ataccacggt
     6661 ttggtaaaat ttacagaaag tttgtaataa agaaattcgg ataaaaatta tggccctgat
     6721 tgataaaaga actctcagat atattaaaga gtacagatgg tcgaacgttt tcgaaatgat
     6781 aagaaacaat cgccgtagtc ctgatatttg tgaagaggat tttacgggga aactcgtcgt
     6841 agttagcgga gctacttcgg gtattggtta ctcaacagtc agaaaatatg cctcaaaagg
     6901 ggccaacatc ctctgtataa acaggaaccc ggagaaatct gagaagctaa aagatgaaat
     6961 agaaaaagaa tttaatgtaa aatgcgatta cataattgcc gatttaagca gtattgagca
     7021 aacacacaat gcatccagca aactgcttga actaactata ccaattgatg tacttattca
     7081 caatgccggt gtatatttta caagaaagca agttactgcc gacggtattg aatatttgtt
     7141 tatggtacat tatctttcat cattcattat taattacata ttaaaagaaa aactaaaagc
     7201 acagggaaat tcgagaatta tcctggtgag ttcagaaggc taccgttttg cagcatgggg
     7261 gctcaggctt gacgacctga actgggaaaa aaggcgatat tcaggtttga aaagctatgg
     7321 ttctgccaaa ttagcccaat tattgaccat gcttcttttt gacgattatt tcagagtgtc
     7381 aggtgtcacg ataaatgcca tgcatccggg agcagtaaaa acagattcgg gacaggagaa
     7441 tggtaaattt tacaaatggt ttaagagaaa tattctggat aaagctctga aaccacctga
     7501 tatttcagca gacaggatat tcaaatgaaa cttgctttct gagcaaactg aggtaatttt
     7561 tagcgtatat cttaaaacaa taaatatctg cattatggat aaaaatcttt tccgctccgg
     7621 gaaaatagtg ccggtatttc tcctggcagc tgctgttttc tccatcagca tactgacggt
     7681 taatgcacaa atctcttcag ggaaagtcaa accccgtatt gtcataacca ctgatcccga
     7741 gcttgatgac agcaactcac ttattcgttt cctgctctac agcaccgacg tggaaattga
     7801 aggtctggtt tatgccagca gtcagtttca ctggaaaggt gatggtaagg gtacccggtg
     7861 gtatgtgccg ggaagagaat atgaccgttt cggaatgaat gaatgtccct gtgaatcatg
     7921 gcgatggtca gaaaattcaa ggcatatcca tgatgttgtg gaagcatatg aaaaggttta
     7981 ccccaatctt ataaaacata atcctgatta tccatcgcct gaatatctga aatcaaagat
     8041 ccggtatgga aatatcgagt tcgatggtga catttcaaaa gattcaccag gatcagattt
     8101 gataaaatcg ctgatacttg atgacaggcc tggtaagtta tttcttgcag catggggagg
     8161 tcacagcacc attgcgcgtg ctctgaaatc gattcaggaa cagtatgaat acacaacaga
     8221 atgggaagcc ttgaaaaaaa agatttcaag gaaggttgtg ttactgcctt cgggtgacca
     8281 ggacgacacc tatgcaaaat acatcaagcc caactggcct gatatagaat acaggcagtt
     8341 caggggtggt cccaattacg gatacggagc acagattaaa gcttcgcctg aagacgcggc
     8401 atatatgaca cctgaatgga tgaaagaaaa cataaccagc cggggtcctc tgggtgaact
     8461 atacagggta tggggcgatg gcaagcagat ggtaaaaggc gacaggctcg actattttgg
     8521 tttgtcgggt tatacagacg agcaactaag aaagatgggt tatattgtat ggatgcctgt
     8581 acagaaaaaa ggatcatggc tcggagaagg cgacaatcat acatttatga atatgcttga
     8641 caatggcctt cgcgcatggg aaaaaggata ctatggcggc tggggtggaa gagtaatcga
     8701 agcccgggaa gcttcccccg ggttaagtgt cagtaaaatg gattatcctg actttttccc
     8761 ccaggcgcag cgcgatttcg ccaaccgcct gaaatggagt gttacagata aatacgaaaa
     8821 tgccaatcac gaacccgtgg tgacagtaga aggacctctg gaattcctcg cctcccccgg
     8881 acagagagtc gccatccacg gaagagtgtc agaccctgac ggaaataaag tagcggttaa
     8941 atggtggcaa tttaatgtag gcacctatcc gggtaaagtt gaaatttctc atcccgattc
     9001 actgaaaaca tacgtaacaa ttcccaaaga tgcagccgga ggacagacca ttcatataat
     9061 tcttgaggcg acagacaatg gtgaaccacc gcttacaagg tatcagagaa ttatcataac
     9121 cataaggtaa taaacaaatt caattaaaaa taaaaaacct gaaccatgaa aaatgtcatt
     9181 ataaacctaa atgctatggt gttgctgatt attttttcag gatatctata tagtttcaga
     9241 aaagaaaaag tgcaacctca gcaacccgtt agcggctatt atgttgcagc atatgtatgg
     9301 ccatcatgtc atgacgatcc gcttggcagg gcaaagctct ggcctgaggg gattggtgaa
     9361 tgggaggtga tcaaaaaggg caccccacgt tttgagggtc attatcagcc aaggcaacct
     9421 ctctggggtt atgaacctga caatgatccc cgtgtggttg aaaaatggat tgatgcagca
     9481 actgaccatg gtgtgaatgt tttcgtatac gactggtact ggtatgaagg gggtcccttt
     9541 cttgagagcg ctctcaacga cggattcctg aaagccagaa acaatgaaaa aatgaagttt
     9601 tacatcatgt gggcatacca tgatgtaagg tacaattact ggaatgttca cctttacagg
     9661 gacaacaaca atatactttt taatgcaaag gtagactggg agaatttcag aatcattgtt
     9721 gagagggtaa taaataatta ttttaagcgt cccaattatt tcaagatcaa tggtgagcct
     9781 gttttttcta ttttcagtgt aaacaaactg attgaaagtt tcggcggcag tgtggaagag
     9841 acccgcaagg cgcttgatta ttttcgtgat gaggtaaaga aagcaggttt tccggggttg
     9901 catcttcaat ggaatcaggg tggtggatcg gttatgacgc aggaggcagc ggaaagattt
     9961 gcagcaagga taaaggaact gggttttgac agttttgcaa tgtacaatat gggaggtttc
    10021 aatgaagatt accttgttta cggtacgaat tccctgaaga tcagggagca gctggatgcg
    10081 attctggaca ttccggtatt tccatgtgta tctattggat gggatgacac gccgcgtttt
    10141 ccggggaagg ggataaagga tgttgtgcat tatcacaaca ctcctgagag ttttgcggca
    10201 ctgttgtcga aagcgaagcg atatgccgac actcatcctg agcagccgaa gctgataacc
    10261 ataaatgcat ggaatgaatg ggttgaaggg agttatctat tgcccgacat gctcaacggg
    10321 tttggatatc tggaagctgt gaaagaggtt tttattgatg gcaggtatga caggtattag
    10381 ggggcaacag gagggttacg tggaaataca ttatattttt gaaagtataa gggaattttc
    10441 gggatttatt taagaagcta agaaataatc taacttagaa atttttttat ttgatatttt
    10501 ttatcatttg ataaattttg ttatttagta ataattacta acaaatattt tttattattt
    10561 gataataatt tttatttgaa acattttact aattgatatt ttttatcatt tgataatatt
    10621 ttttacctga taatttttac tacctgataa aacgtactat ttaataattc attctatttg
    10681 aaattttttt tcaaaaaata aaaattctaa aaaattttac taactttgtt aacactaaac
    10741 caaaaataaa aatactatgg ccgatattac tgctcctaaa atacttatgg atcacggact
    10801 taaagtaacc cctcaaagaa ccctcatcct cgaagctctt ataagcctcg atgaacaccc
    10861 ttcctacgaa atcatcatga aattcatgag attcagatac cccaatatct ctcttgcaac
    10921 tatctataat acactcaaac tttttaaaga aaaaggtatt ataaaacaga ttgcagctgg
    10981 cgacgaagaa atacgattcg accccgtgca aaaaaaacac caccacctat attacaaaga
    11041 caccgataaa ctcgaagatt tcgaagataa agaactcgac agaatcctta aaaactactt
    11101 caataaaaaa caaatacccg gatttaaaat agaggatata tccataatgt tgaccggaca
    11161 ctcagaaaaa acccagacac ccgaaaaatc aaaacaaaaa acacagaaaa aaattattaa
    11221 atagcctgca gaacaataac ccgggcaaat tgttaattta aatatgcccg caatcatacc
    11281 agaacttaat aaaactattg tcaaactata aattatatga actatggcag aaattggaag
    11341 atcaatcgtg aaaatggatg ttgacaagct gatcaacctc cttaacaaag cactctccga
    11401 cgaatggctg gcctactacc agtactggat aggtgcaaaa gttgtaaaag gccccatgaa
    11461 agacgcagtt attagtgaac tgcaactgca tgcaaccgaa gaacttaccc acgccgacct
    11521 gctggccaca agaatcgtgc aacttggcgg aaccccggtc ctctccccgc aggaatggct
    11581 gaaaattacc aactgcggat acgatgcacc tgaagatcct ttcgtgggtg taatactcga
    11641 ccagaacatc aaaggcgaac agtgcgcaat aagcacatac aataaactgc ttgacataac
    11701 aaaagatgcc gacccaatta cctataacat aatcctccag ataataaccg atgaggtcga
    11761 acatgaggag gaccttgctg cacttaaaga agatctagaa ttaatgcttg caaaacataa
    11821 ataatattaa atccttaaca atatgtgcga agaatttgca gtatcaatgc cgagaatcgg
    11881 cgacaaagcc cctgaattca aagctgtaac aacacagggc gagataaact tcccagctga
    11941 tttcaaagga agctgggtga tcttctttag ccacccggcc gactttacac cggtatgcac
    12001 ctcagagttc atgaccttcg ccacgatgga gaaaaaattc aacgaggcca actgcaaact
    12061 ggtgggactc tcagtcgacg gactctacag ccacatcgcc tggcttagaa ctatcaaaga
    12121 aaaaatcgaa tacaaaggga tgaaaaacgt ggaggtcaca ttcccactta tagaagatat
    12181 cacaatggag atatcaaaga aatacggaat gatacaacca ggagaaagct ccacaaaagc
    12241 cgtaagagca gtattcttta tcgacccgaa cggcatcatc cgtactatca tctattaccc
    12301 actgagcctc ggccgtaact tcgaagaact ttaccgtgtt ctcataggcc ttcagacagc
    12361 tgatgcattc tcagtggccc tgcctgccga ctggcaacca ggcgatgatg ttattgtccc
    12421 acctgctggc tcatgtggtg ttgccaaaga acggatggaa ggtaaaaaag aagacataaa
    12481 atgctacgac tggttcttct gtactaaaaa gttacctaaa gaatccatct tcaaaaagat
    12541 actgaataag taaaaacagt atcccatcag caactgcagg aagaacaggc cacataactg
    12601 cctgttcttc ttttatttta caatgcaaaa taaattcgcc gtcttaatat ttacaaaata
    12661 agtcttaaac tattgttatc tttgcaacaa aatgaacaat attctcaaat aactgtttag
    12721 aaatttattc aataattaac ctgttcaaaa aaatcttaaa attatgatcg aaatctattt
    12781 tgaaggcggt aaagttgtaa ccgcaaacgt ccatggacat actataagaa ctgatcagcc
    12841 tgttgaaagc ggaggaaaca acagcgcacc tgcgcctttt gacctttttc tggcctcaat
    12901 aggaacctgc gcaggcattt acgtcaaatc tttctgcgac agaagaggca taccctccga
    12961 tggaataaaa attatccaga caatggaagt tgaccaggaa aaaaggctgc cttctttatt
    13021 taaacttgaa atacaacttc cccccgactt cccggaaaaa tatactgaag cagttataaa
    13081 tgctgcagat ctttgtcttg taaaaaagac tatatataat caaccaaaat ttgaaattac
    13141 tgcaacaaaa aaataaaact atgaaaattg cagtacctgt cacctccgac aataagctcg
    13201 atagccattt cgggcattgc agacatttca aaatcttttc tgtaaaaaat gaaaatgaaa
    13261 ttgaatgcat caagaaaatt tcacccgaaa atggccatgg atgcaaatcg ggaatagttt
    13321 cactgctggc agcagaaggc gtggaagttc tgcttgccgg tggtattgga gaaggtgcag
    13381 tcaataacct gaactccgca ggaatcaaag ttattagagg ctgtgcagga gagcctgacg
    13441 accttgtgag gctttatatt gaaggtaaga ttgatgacag cggagaatca tgcaggcacc
    13501 cccgtcatca tcaacacgaa ggtcaacaag gtacctgcca ccacagttag ccccaggtta
    13561 aaactactta cccggttaag tcatgataaa atatcatgac taatattata tttacgcctg
    13621 ttcttactga cgttgggcat aaaattatgg agcacataaa accgggcgag cttaaacagg
    13681 tatggctgaa ggcttctgta gttggcagca tctgggcttc tttcgaaatc atccttggca
    13741 gttttttaca taacctcaaa atccctatat cgggaactat tctttcattt ataggcgtct
    13801 ggatactgat ttcatttcaa cttgtatgga aagataaagg ccttgtatgg agggctgggc
    13861 tgatatgcgc acttatgaaa tccatatcac ccagtgcaat aatactgggt ccgatgatag
    13921 gtatctttac cgaatcgctg attattgaaa taacaacccg gctgcttggc agaaactttc
    13981 ttgcatatat catcggaggc gcacttgcag tcctcagcac cctggttcag aaaataatta
    14041 acctgataat actttacgga ctcgacctga ttaaaatact tgataacctc tacagatttg
    14101 ctgtgaaaca gatcggaatg gaacaactca atccggttta cgttattgca gccattaccg
    14161 ctctatacct gctggcaggc atggcaggcg ctacgggggg atttttgacc gggaaaagat
    14221 atcttaaatt aaaacgcctc caggaaacag aagaaaaaat cacccttaag ccgggaactg
    14281 aacttttcga gaaaacctct gaggataaat attctatact ttttctgctc ctgaatctgt
    14341 ttgccattat cgggatactc ctccttctga attataacct tttaattatt gcaggaattg
    14401 ttgcggttat ttattcaaca ttctgcatac tcacctataa aaattcactc cgcaggctga
    14461 agaaacccgg cttctggatc agtttcttta tcattacttt ctcggccgca tttatctgga
    14521 actggttctc cgacggcatc ttctttacaa ccgagggatt tctggtagga ctgaaaatga
    14581 atgcaagggc aacggtgatg attataggtt ttgcctccat aagtgccgaa ctgaaaaatc
    14641 cagttatcaa atcaatgctt tacaacaggg ggtttgcatc actttaccaa tcgctgaatc
    14701 tctcattctc agctctcccc taccttattt caacattgac aatcaaagaa gaacagggaa
    14761 aatttccggg caaaaaatca ctgcacggaa tattgagccg ggccgataac ctgtttaacg
    14821 tttttgaaaa cgaatatcgc aagagaccgt cgattgtgat tattaccgga gaggtccatg
    14881 ccggaaaaac cacctatgcc atgaagataa tcaatgctct aagggaaaga aatatacagg
    14941 catgtggctt tcttgccctg gcggttaatg aaaacggaga aagaaaagga ttcaatcttc
    15001 tcgatcttga aacaggagaa gaaaaagaac tctgctccac ctcagaaaca ccgggcagtt
    15061 taaaatcagg gcgattctat ttccggcgtg aaaccttaga aaaaggatta aagatattaa
    15121 caaatatacc acacacctgc ccctgcgtgg ttatagatga aataggcccg ctcgaactgg
    15181 ccggaggagg ctggagcatg gctatagaag atctctgcag aaaaaatgtt tcaccacaga
    15241 tatgggtggt aagaaaacca atactccacc agttaatacg acgctggaat accggagata
    15301 tttacatttt tgatattcaa accgacagca tcaaagatgc tgtggaaaaa gttctggaac
    15361 tctccctgaa acaaaaacct gcagggactt agaattattt ctcaacccgg gttttgcagg
    15421 tgttaatacc aaatagggta taaattccgc aaaaacctgt tattgaagta ataagaagta
    15481 tcaccgcaaa aacaagtaaa actattccta acgttccgct gataacattt gtaaagtata
    15541 aaatcccaat cagcaaggct ataattagcc ttattattct gtcaagtttt cctgtattct
    15601 ttttcataat cctttgattt ttatttttct aataaaatta attataactg ataaacaatt
    15661 caatctgcat taagttcaaa ttgatgactt tttgtatttt tattagttct aaaatttact
    15721 ccttcttccc cgggggaagt ttggatgata gtaagtaaag catgaacgaa tctttaaaaa
    15781 gtaaaaactt ataatttgat aatcaaaaat atataaaatt attttcacat gaaaaaaata
    15841 cacttgtcct ggttggtgat attgataatt tctacaatat tctttcaaga atgtcgcaat
    15901 aaatctgaat ataatgagac gataaaaagg atcgcagcat tggttgagga aggagcactt
    15961 gaaaaagctg caatgattgc cgattcactc aaaaatgcga caagagatga tgatttgaga
    16021 tggaaagccg attcgctcaa tgaaataaca aaacgtatcc ggctcgattt ttcagttacc
    16081 caaaacggta ttgacacaat tcttggaaag ctggtacagg gctacagtga atctgacaga
    16141 aaaatatggg aagaaaacgg atggctggaa tacagaatca tcgacggtga aaagcgttac
    16201 ttcaacaggg cggctaaaaa tcttatcctt cttagaaact tttatcaaaa caggcagtac
    16261 agagattctc taactgcgag ggatccacga attgttttca ggaaaaacca tacagccgat
    16321 attataagag tttccggaaa gagccctgtt ccggttatgc ccatccagat agaggtaaca
    16381 tatacaatca ctgtcaaacc cgatgctgtt cctgctggtt caacaatacg atgctggatg
    16441 ccttacccga aagagaacaa taagaggcag acagaagtct atcttcttgg catctctaac
    16501 gagaattatt ttctgctggc acccgattcg gtagttcata aaacggttta tatggaagaa
    16561 aaagccagaa gaggcatccc aactgtcttt cagatagcat tctcatattt ttcgtatggc
    16621 cagtatttcg atccggaaaa tatgaaaata cttccatacg ataagaattc tgaaatttat
    16681 agaaaatata cacgggaaca gccaccacat atatgcttca ccgacagagt aaagcatctt
    16741 gcagacagtc tggccggtaa tgaatcgaac ccttacgaaa tagtgaagag attctactac
    16801 tggtttaata ataatattcc atgggcgggt gctcttgaat attcaatcat accaaatatt
    16861 ccggaatata cgctgaaaaa ccggagaggt gactgcggca tgcagacatt ccttctgatg
    16921 tcgatgttga ggtataaagg tatacccgtt aaatggcaga gcggctggat ggtgcctcca
    16981 cagggcgaaa accttcacga ctggtgcgaa gtgtattacg aaggaacagg atgggttccg
    17041 cttgacattt cttacagtct ccagtattcg aacaaccaga aacttaaaga attctatatt
    17101 accggaatag attcataccg gctaattata aacgacgata taggaggcaa actttaccct
    17161 gagaaaaaat atctgcgaag cgaacctttt gatttccaga gaggtgaagt cgaatgggaa
    17221 ggcggtaatc tctattttga ccagtgggat tatgacattc aggtcaaaat aaaaccacta
    17281 cccggaacca ggatttaatg aataaggagt tattttaaac ctgctatgat aatgaataat
    17341 acaaagttat taaggattct catcttttta ataacagcat tgatggtggt ggccgagggt
    17401 tgcaggttcc ctgttggtat ccggcttgtt aaccagatag attcaatttc gaagaaatgg
    17461 gtacccgaca gccgcgaagg tgtttttaag gtggaaattt cccgtaaagg cacaatactt
    17521 atgctgaagg gggaaacaga tattcctgag gcaaaagctg atataataaa ccttctgaag
    17581 agcagaaaaa caaaattcat cgacagcctt atagtgctgc ccgacacttc tgtaattaaa
    17641 aaaccatggg gacttgtagc agtgagcgtt tgtaatatca gatctgctcc tgaccacagt
    17701 gctgaactta catctcaggc actgatggga actccggtaa aggttcttaa aaagaaaaat
    17761 agctggtatt ttattcaaac acccgac
//
LOCUS       DAOSNB010000017        17087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3372_length_17087_cov_7.039925, whole genome shotgun sequence.
ACCESSION   DAOSNB010000017 DAOSNB010000000
VERSION     DAOSNB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3372_length_17087_cov_7.039925"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..861
                     /locus_tag="PLG42_01635"
     CDS             328..861
                     /locus_tag="PLG42_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU29943.1"
                     /translation="MHGQTLHTNGSWYKEKTSAGNIIWFKQYKTKSCRSCPVRSHCTR
                     SKKERLIHRSEYADYYERNRTNTIEKEHLYKRRQTIVEHLYGTIKRQWGYSYVLTKKG
                     ISRASADVGLMFIAYNLRRIGNILTMNVLKEYLRMLSSLFMCLFDLKWVILKRSRGLC
                     YVITARTVKINLRLKPA"
     gene            2224..3084
                     /locus_tag="PLG42_01640"
     CDS             2224..3084
                     /locus_tag="PLG42_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29944.1"
                     /translation="MIQKSRWLYPGLIAFSIILNSCSKPLEWPESPLYVFDTGKLNTI
                     YPADKESAMSHIVGHYAHFDVVAYEDTTTRTTMRTFIISYGFTNFYLEDGKLMQSDRF
                     VHAEQKLSRKNARSALRDEAVQAIKPRVQEVDLSLKDGVWNIYRPESPSLLGIEGDPQ
                     KSLSTDPNDPNLTDPDKDGHPGVTVQISVGKFFSGEIYITRREVFRNYLSLNYNGTLT
                     GYVVDHSEQFVVGASKKILNQKSNNSQHPDKGLNPVLLVKVDPDIDTYEELLQVRDKI
                     FPKEPEFKTK"
     gene            3123..3596
                     /locus_tag="PLG42_01645"
     CDS             3123..3596
                     /locus_tag="PLG42_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOU29945.1"
                     /translation="MIEQQTEKENSTTPFVLNAESIKELWSKTYNTEGKPDWSHILPY
                     YDDNVYFRDSIQAIHGKEEFISMTKRLTDRSKDLSMKIVRTGQHGKNIFIEWEMTIKY
                     KKSPSSVLYGSSRVTLNDEGKIIEQRDYYDLWGDIFDNIPRFGKAYRKFMKKKFG"
     gene            3647..4333
                     /locus_tag="PLG42_01650"
     CDS             3647..4333
                     /locus_tag="PLG42_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU29946.1"
                     /translation="MKKTSFSIMFISFLLCGALSAQTGARFGLKAGFNLATQYGIKPA
                     DDLYTVSSTGRNGFAGGIMIYYPITESVGIQQELLYVMKGSGQDVTITTPLNINTKSE
                     YKMNYFEMPMVLKYRFVNIKDFGIYGNTGIALTLLINGDYNITTTINTGIPPLIVTKE
                     SGNMKGLDMFDYSFVYGLGTDFKLLNKDFFFETRMTIGWNTLAMPNATGADPIPLRNQ
                     AYTFTLGLYF"
     gene            4397..5368
                     /locus_tag="PLG42_01655"
     CDS             4397..5368
                     /locus_tag="PLG42_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU29947.1"
                     /translation="MKNKNFKKYFKAYQWSNIVSMIKNNSRKPKICTSDFKGRLVVIS
                     GATSGIGYHTARKYASCGANILCINRNEQKSEALKTEIERDFGVSCKYIIADLSNMKD
                     IFTAAEKLRSIETPIDVLIHNAGVYLTKRELTPEGFDKVFVVHHLSSFVINILLSEKL
                     KSQEKARIILVNSEGHRFAAWGLQLSDLNWEIRRYSGLKSYGSAKLAQLLSMMLLADY
                     YKPSGVTINAMHPGAVRSDTGRENGAAYRWVKKHLFDKTLKPTDISAESIYYLGVSEE
                     VSGVSGKFFNLTTEEEPAPPALDRESAQELWAKSVEMTGVKDIIGKS"
     gene            5374..6969
                     /locus_tag="PLG42_01660"
     CDS             5374..6969
                     /locus_tag="PLG42_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU29948.1"
                     /translation="MTENKYDSVIVGGGIAGLTCGAFLAKEGHHVLIIEKNKECGGLV
                     NSFSHNSFTFDTGIRALENAGIIFPMLKELGVELDVVKSHVTVGVENELIDILDIDSL
                     NEYSNLLKRLYPESKDEIDSVIRIIRKVMKHMDVLYGVDNPNFKDIKNDRRYLFCELL
                     PWLPKFLLTLRKINRMNVPVEPYLETIVKNQSLRDIISQHFFRNTPAFFALSYFSLYL
                     DYFYPKGGVGKLAEAVENSFLKQGGEILRETTIDRVTLNEKYVSDQNGIKYHFNTLVW
                     AADLKTFYRITTTEGLPSEVSRDFEKVKNRLTAGRGGDSVFTLFLEVDIPTEYFKKIG
                     KGHFFYSPSRKGLGDLRWNSINNIIKSSQKPDRSEILNWLDKFLAFNTYEISIPALKD
                     PDLAPEGKTGLIISFLADYDLFKKVHDEGWHKEFITELEERIIRVLSDSVYPVLKEKV
                     IDRFSFTPLSYEKRVGSSEGAITGWSFCEPIPVINKITAAGKAVLTPVPSIYQAGQWT
                     YSPAGVPMSILTGKLAAQRILSS"
     gene            complement(7052..8044)
                     /locus_tag="PLG42_01665"
     CDS             complement(7052..8044)
                     /locus_tag="PLG42_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29949.1"
                     /translation="MEIQNDEKFIKRVESLINPMEVSNLLFFQKLKGLLPTAVLKFML
                     HRASKNTPYIGFIIDPYSLFLFFKLADIELAKSMLPGRYELSKTRLFADDVPDYYMGI
                     GNLSTRASTFWGTRMEAYLIAKDKETGLLSFIFIDILSNTIIALPTKGIADPNCERAI
                     LTTSSQGEVFFDIEEKRTKRKLQFKGIIKRGKIRILDQQLWLMGNTSIGHCKSYVKGE
                     DIPFAVIFDPAEVKEALDIPVEDIHIKENTLFPGLARQEISAAICFPYAQHYIADSPG
                     CYTKIKDREELISMYNRIADSGEFKTFSARTIINQVVAGIVITAIAVLLLSLIF"
     gene            8261..9679
                     /locus_tag="PLG42_01670"
     CDS             8261..9679
                     /locus_tag="PLG42_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU29950.1"
                     /translation="MKILLTGATGYIAQRLLPVLLRNGHEVICCVRDRIRFNDKYNSP
                     LLNVIEADFLKKETLTVIPKDIDAAYYLIHSMSLSKGDFEILESISAQNFKDIIQQTS
                     ARQVIYLSGIVNEARLSKHLSSRKNVEAILTGGSYSLTTLRAGIIIGSGSASFEIIRD
                     LVEKLPLMIAPKWLNTLSQPIAIRNVIEFLSGVLLNESTFNHSYDIGGPDILTYKQML
                     LGFAKVRGLRRYIGIVPVMTPKLSSYWLYFITSTSYRLAQNLVDSMKVEVICKENNLG
                     EILGIKPVGYDEAVAEALEKIELNQVISSWKDALTSDTLNGGILKHLDVPAYGCYFDY
                     RKRKVRDPECTLQKIWSVGGTVGWYYGDWLWAIRGFIDKLFGGVGLRRGRKSQSEISP
                     GEALDFWRVLAADKSEKRLLLYAEMKLPGEAWLEFKIDKDNILHQTATFRPLGLTGRL
                     YWYLVLPLHFFIFRGMIKKLAC"
     gene            9969..10880
                     /locus_tag="PLG42_01675"
     CDS             9969..10880
                     /locus_tag="PLG42_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU29951.1"
                     /translation="MVNFKLIGNKIAEARKKVNISQAQLAQRLFISSQAVGKWERGES
                     MPDIITFNRLAEILGVDLNYFSEGLFPVPAEKVLIDSSENKTNDLLSAKPKINRSWDM
                     SRGNWMDADFSGLKNLHERFSSSNMLRCKFVGSDMSGLLLKSNNVQTCDFSGSDFSRS
                     QIQNSNLSNNLFKDCPMKETEFSQTNIIDCDFTGADFTGVIIKAGGFGKNTIVDAIWN
                     CTSFVDSHLADVIFTGTLKDCSFENCSFTKLIFKNATILNTFFKNKSLKRIQFIDCQA
                     DRITYEFLKNGKANLTGITLIPKGDEK"
     gene            10983..11795
                     /locus_tag="PLG42_01680"
     CDS             10983..11795
                     /locus_tag="PLG42_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29952.1"
                     /translation="MNKIAYCISILIIGIVAYLFLFGKLFAFSPIIIGFDKHESKNAI
                     FYIQKDSKYNDFELIDTLIPSVENFHELNFIRKPRIFTFQDSLTYIHHSLSKARFCAF
                     SSGRLFISPWALKEAKQGKISIPIYTKHELSHVLILQNKGIFSELKFPEWLLEGIAVY
                     SANQLGTSFYPGKEETYKAIAQGNFMPPFDFKTKRESKINLDVKYRITFMYSEFACIV
                     DYLIKKYGKDKFLSYMKALIKENDHDEIFKKIYGVDFDESIEEFLRYVTTGI"
     gene            11908..13113
                     /locus_tag="PLG42_01685"
     CDS             11908..13113
                     /locus_tag="PLG42_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU29953.1"
                     /translation="MINRKLITSFFLIVAIVVPAYGQANTQKALKDAYAGKFLIGAAN
                     DLTKISDAEAANIKMHYDIITPENCMKPQPIHPSEDKYNFVTSDAMVEWCQKNGLKVW
                     GHTLAWHSQSPAWFFQEKNAATGEAAAPTPQPATTPAAAPDPNGPRPGMGGFGDRANT
                     GPQASREVILERLKNHIMTVVGRYKGKITGWDVFNESIADGGDGKTENLRTSSWYKAV
                     GPDVITLAFKWAHEADPNAQLYYNDYNIEQGAVKNTGKHASSILLLKRLIAEGAPITG
                     VGIQGHWHLDTNLEDVEKAIENYASLGLKVSITELDVTATGTNSGAFGAGQGNRTIPP
                     ENYQKQAEVYKKLFEIFMRHADVIDRVTFWGLSDTRSWRRGQDALLFDGQLNPKPAFN
                     AVIEASSSK"
     gene            13280..13762
                     /locus_tag="PLG42_01690"
     CDS             13280..13762
                     /locus_tag="PLG42_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29954.1"
                     /translation="MKSIIAGINLLILILIVSCGRDLDQSKRYSDMKSEYDSLIAQAN
                     LIINEQKTIADSLKNIRDDIVRNKALNYKLPEINRKITNAREIESNIEEIRKHLEAIR
                     LFANNGEKVTKRIIYFKRKYIVYSITIQIHDENIMIRDITGDVSAKQYLSRYLSSERK
                     "
     gene            14065..16608
                     /locus_tag="PLG42_01695"
     CDS             14065..16608
                     /locus_tag="PLG42_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HOU29955.1"
                     /translation="MRRLSFLFLVLLLAVACNKGPNQDSNQDLKLNDLGYFETQGVNV
                     LVYNNLFTGGFNDEKTAGIELIHHGVRTAQGGAVRLSNTPEQWDLVPAIPIRTVDTLA
                     NKIESTLRYEDYDFDSRVIVSARGKGVEISVYLDKPVPAALEGAAGFNLEFLPSQYWN
                     KTFLMDGRPNRFPRYVVGNTITRPNSEKIKQFKGYKTYDDRGTGKFIDPLPLETGRTI
                     LMAPEDPERLVKITSEDADLMLFDGRILAQNGWFVVRSLLPAGKTGKVLTWTVEPNAI
                     KGWIREPNIGFSQVGYIPSQPKVSVIELDKKDKPLAKASIYKVGDDGNATEVFSGKIV
                     PWGDYFKYHYVKFDFSSVNTPGIYYIQYGDIKTNNFLIENNVYDKMTDATSDIWIPIH
                     MNHMFVNEAYRVWHGEPFKEGYLQAPAPTDHFDMHRQGPTTDTKYKGLELIPGLNIGG
                     FFDAGDFDIETGSNIGVVQNFVQTWEYFKPLRDQTFVDQKQRYVDLHRPDGTPDILQF
                     IEHGTLNLLAQAEIIGHMASTLSNSVLDNYHHLGDAASITDGLPYNPNLGPYEVARDG
                     RSSGLKDDMWAFTSRNPGTDLRAATMFAAASRALKGYNDNLSARALKQSKRLLKEATE
                     LLAKQPQGPAGRSFGSADMGTNLQLYISTGEQQYIDKFQELLWPALERNVSGSILTAL
                     HAIPHMDASYKEKLRPYVVKYKEYVDEMEQDNPYGVPIGLGNWAGSGGVVSFGTTICF
                     ANMHFPDIIDASHAFKTTNWLFGCHPYHNYSLVATLGATRPKAVFYGNNRADFSFIPG
                     NVAPGILFRQPDHFENYDDWPFLWGQNEGTIGGNTSYLIFGSAFKNMLK"
     gene            16768..>17087
                     /locus_tag="PLG42_01700"
     CDS             16768..>17087
                     /locus_tag="PLG42_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007211527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOU29956.1"
                     /translation="MNIKPKTFLSAVITVILAVSGLQGYAAMSGDKTDSAQQSDNKRS
                     IAPYFIRATKAKKTPDTDGFIQRWLLLEPINKPNRSNTVFTDSYIRTAFTTEYFPNQF
                     TGIP"
BASE COUNT         5503 a         3294 c         3478 g         4812 t
ORIGIN      
        1 aaactgcaga atccttatta aaatcaaaca tgcggaattt aggagctatc gggacataca
       61 aggacaaaac agaacccaaa ttaatctcct tcctgtctcc ctggatcaga aaatagatgc
      121 cgataataaa gtgcagataa ttgacctgtt tgtcgatagc ctttctataa aggttttcat
      181 acgggttcag aacttaagac agcacatgat cttggcgttg agacgatagt agcaataccg
      241 ggagtacctt ccaccagtca ggcacctgat ccgctgttca actacgagtt tttcaggtat
      301 aacagggagg atgataatta tacctgtctg catggacaga cactccacac caacggatca
      361 tggtataaag agaaaacaag tgcaggcaat attatctggt ttaagcagta taaaacgaag
      421 tcatgcagaa gttgtccggt acgttcacat tgtaccagat cgaagaagga gaggcttata
      481 cacagaagcg aatatgctga ttactacgag aggaaccgta ccaacaccat tgagaaggaa
      541 catctttaca aacgccgtca aacaattgtg gaacacctgt atggaactat aaaacgacaa
      601 tgggggtaca gctatgttct tactaaaaaa ggaataagca gggcaagcgc cgatgtgggc
      661 cttatgttta ttgcatataa tctcagaaga attgggaata ttttgaccat gaatgtgctg
      721 aaggagtatc tgaggatgct ttcttccctg tttatgtgtc tgtttgacct caaatgggtc
      781 attttaaagc gttccagggg cttatgttat gtaattacgg ccaggactgt gaaaatcaac
      841 ctgaggctta agcctgctta aaaaaggtca gattctgtgg aataatagag attaatagac
      901 aaactcccgt tagcggtaat agtgatcgct gtggtaatct tgactaattt gaatccttat
      961 cttggaacaa catgttgaca ttgccaaatt tttaatactt attttcattt agagttctat
     1021 gaataaattt aaatttgaac atcacagaca ggaaatttta ccattaccaa aatttctttt
     1081 taggcttagt aagtatctat tgttttgttt aatattaatc gccttttcac ttctaatagg
     1141 gatttcaggg tatcattttt ttggaaaact cgacttaatt gacagttttc atatgtccag
     1201 tatgattttg actggaatgg gacctgtcaa tgaaatgaca actgatagtg cgaagatttt
     1261 ttcttcactc tatgctttat atagcgggat agcattttta agtattacag ctgtattctt
     1321 ctctccaata tttcatagaa taatgcacat tttgcatttt gaaggaaata atgaaaccaa
     1381 ataaactaca gcctctaaca cgaacggtag ggtcaataag cttgtcacac tttctgcttt
     1441 tgttcctgct cccaagacag atttgtaatg gtttttgcac ccggatacgc gcatcagcct
     1501 gcaatcaaaa tcctgcaccg tgacatcccg atagctatcg ggacgggaca gattcgtggc
     1561 agtgctgtcg tcgggtaaag cttacgaaat tataccgccg ggcctttagc acatatattt
     1621 ggcaattcgt aacttagccc ttgcgccgct acatattaat tttattctga cagtaaaaaa
     1681 ttcaacactt ttaccgggac ttgccaacgc acgacagaca attggccctc ccgccgtcac
     1741 caaaaacttc tgtcatttaa ttaacaatac tgctttctac ctggactctc ctatcgcaca
     1801 acttaaaata catgtgctaa cagctaaaag ccaattttta tgatccggct agatttaatc
     1861 cgaactaaaa caagactccg gaattgtcga atgttcgttt tttaatagat ttgtaaaaac
     1921 aaccaaaatg aatcaatcag agattaaaaa agtaatcttg agccatttaa aagactatga
     1981 tccaattaag attggcattt ttggttcttt tgcatgaggt gagaataaaa atgatagtga
     2041 tatagatatc cttgttgagt ttaaggaatc tccaacatta ctcactctga ttaaattaga
     2101 aaatgaccta tcagagattc ttgggattaa agttgtatct cgtcctgaaa taggttgaca
     2161 ctccggattt ggaattctat ttactttaag atagatttgc gagtagtata atattcgtaa
     2221 attatgatac aaaaaagcag atggctttat cctggattaa tagctttttc cataatctta
     2281 aattcctgtt caaagcctct ggaatggcca gaatcgccac tgtatgtctt tgatacaggg
     2341 aagctgaata caatatatcc cgcagataaa gaaagtgcaa tgtcgcatat tgtaggccat
     2401 tatgctcatt ttgatgtcgt agcttatgaa gatacaacga ccagaacaac aatgcgcaca
     2461 tttattattt cttatggttt tactaatttc tatctggaag atggcaaact gatgcagtct
     2521 gaccggtttg tacatgcaga acaaaaacta agcagaaaaa atgccaggtc ggcattgagg
     2581 gatgaggcag ttcaggcaat caagccccgt gttcaggagg ttgatttatc tcttaaagat
     2641 ggtgtctgga atatctacag accagaaagc ccaagcctcc tgggtattga aggagacccg
     2701 caaaaatctc tttcaacaga tcccaatgac cccaacttaa cagacccaga caaagacgga
     2761 catcccgggg ttacagtcca aataagcgtg gggaaattct tcagtggtga gatttatata
     2821 acccgccgtg aagtattcag aaattatctt tcgcttaact ataatggtac gctaactggc
     2881 tatgtggtgg accattcaga acagtttgtt gtaggtgcat caaagaaaat actgaatcag
     2941 aaatcaaaca attcccagca tcctgataag ggcttaaacc cggttttgct agttaaggtt
     3001 gaccctgata ttgacacata cgaggaatta ttgcaggtaa gagataaaat ctttccgaag
     3061 gaaccggagt tcaaaacaaa ataaataata aagatcctgt ctgacgatta taatatttca
     3121 atatgattga gcaacaaaca gaaaaggaaa acagtaccac accctttgtc cttaatgcag
     3181 aaagcataaa agagttatgg tcaaaaacct ataataccga aggtaagcca gactggtcac
     3241 acattcttcc ttattatgat gataatgttt atttccggga ttcgatccag gcaattcacg
     3301 gaaaagaaga gtttatttca atgactaaac gtctcacaga ccgttcaaag gacttgtcga
     3361 tgaaaattgt aaggaccggc cagcacggaa agaatatttt catagaatgg gaaatgacaa
     3421 taaagtataa gaaaagccca agttctgttc tttacggatc tagccgcgta acactgaacg
     3481 acgaggggaa aatcatcgaa caaagagatt attacgacct ctggggcgat atttttgaca
     3541 acatccccag gtttggcaag gcttacagaa aattcatgaa aaagaagttc ggatagtctt
     3601 aattgaacaa aatgtttgtt ccgtccgttt attaaacaaa aaatttatga aaaagacatc
     3661 attttccatc atgtttatat ccttcctgct ttgcggggca ttgtcagcac agacaggagc
     3721 ccgttttggc ctgaaggctg gatttaatct tgcaactcaa tacggcatca agcctgccga
     3781 cgacttgtac acagtcagct caacaggaag gaatggcttt gccggaggaa taatgatata
     3841 ctaccctata acagagtcgg taggtattca gcaggagctt ttatatgtta tgaaagggtc
     3901 gggtcaggat gtaacaatta ctacccctct gaacataaat acaaagtcgg aatataagat
     3961 gaactacttt gaaatgccta tggttctaaa gtacaggttc gtcaatatca aggattttgg
     4021 catctacggt aacaccggta tcgccttaac tcttttaata aacggcgatt acaatataac
     4081 aacaacaatc aataccggca tccctccgct aattgtaaca aaagagtcag gcaacatgaa
     4141 agggttggat atgtttgatt attcgtttgt atacggttta gggactgact ttaagcttct
     4201 taataaagat ttcttctttg agacccgcat gacaatcgga tggaatactc tggcaatgcc
     4261 taatgcgact ggcgccgatc ctattccgct gcgcaaccag gcttatactt ttacacttgg
     4321 tctttatttc tagactccca tcactgttta agtgataagt aatctgatat gtctttctta
     4381 tttgcgtgta agacctatga agaataaaaa ttttaaaaaa tatttcaagg cgtatcagtg
     4441 gtcgaacatt gtgtcaatga tcaaaaacaa cagtcgaaag ccgaaaatct gtacaagcga
     4501 tttcaaaggc agactggtgg taataagtgg cgcaacatcg ggaatcggat atcatacggc
     4561 ccggaaatat gcatcatgcg gggcaaacat tctgtgcatc aaccgtaatg agcaaaaaag
     4621 cgaagctctt aaaaccgaaa tagaaaggga ctttggtgta agctgtaaat acatcattgc
     4681 cgatctgagc aatatgaaag acattttcac tgctgctgaa aaacttcgaa gtattgagac
     4741 tcctatagat gtgctaatcc ataatgcagg agtttatctt acaaagagag agcttacgcc
     4801 ggaaggattc gacaaggttt ttgttgtgca tcatctttcc tcttttgtta ttaatatcct
     4861 tctcagcgaa aaactaaaat cgcaggaaaa ggcaagaata atattagtaa actcggaagg
     4921 ccacaggttt gccgcctggg gcctgcaact aagcgaccta aactgggaga tacgcagata
     4981 ttctggattg aaatcatacg gctcggccaa gctggcccaa ctgctttcta tgatgctgtt
     5041 ggcagattac tacaagccgt cgggtgtaac aattaatgcc atgcacccgg gtgctgtcag
     5101 gtcagatacc ggaagagaaa atggagctgc ctatcgctgg gttaagaaac acctcttcga
     5161 taaaacactg aaaccgactg atatctcagc tgaatcgatt tattatcttg gagtctcgga
     5221 agaagtaagc ggagtaagcg gaaagttctt taaccttacg acagaagaag agcctgcacc
     5281 accggcattg gatagagagt cggcccagga attgtgggca aaaagtgtgg aaatgacagg
     5341 agtaaaggat ataattggca aatcataata agtatgactg aaaataaata tgattcagtc
     5401 attgtaggtg gcgggatagc agggcttacc tgcggggcct ttctcgctaa agaaggccac
     5461 catgttctga ttattgaaaa gaacaaggaa tgtggaggac ttgtgaattc cttttcacac
     5521 aatagtttta cttttgatac agggataagg gctctcgaaa atgctggcat tatttttcca
     5581 atgctgaaag aactgggggt tgagctcgat gtggttaaaa gccatgtcac tgtaggtgtt
     5641 gaaaatgaat taattgatat attggacatc gatagcctta atgaatacag taatctcctg
     5701 aaaagactgt atcccgaaag caaagatgaa attgacagtg ttataaggat aatccggaag
     5761 gtgatgaagc atatggatgt cctttacggt gttgataatc cgaattttaa agatataaag
     5821 aacgacagaa ggtatctgtt ctgcgaactt ctgccgtggc ttccgaaatt ccttttaaca
     5881 ttgagaaaaa tcaaccggat gaatgtgccg gttgagccat atcttgaaac aattgtaaaa
     5941 aaccaatccc tcagagatat tatctcccag cattttttca ggaacacacc tgcctttttt
     6001 gctctgagct acttttctct gtacctcgac tatttctacc caaaaggcgg ggttggcaaa
     6061 ctggctgaag ctgttgaaaa cagttttctt aagcagggtg gcgaaatatt aagggagacc
     6121 accattgata gagtaacact gaacgaaaaa tatgttagtg accagaacgg cataaaatat
     6181 cattttaata ccctggtatg ggccgccgat ctaaaaactt tctacaggat tacaacaaca
     6241 gagggattgc cgtcagaggt cagccgtgat tttgaaaagg ttaaaaacag attgacagcg
     6301 gggaggggtg gcgactctgt ttttactctt tttctggagg ttgatatacc aacagaatac
     6361 tttaaaaaaa taggaaaagg gcacttcttt tattcccctt cgcgaaaagg acttggtgat
     6421 ttaagatgga acagtataaa taatattata aagtcatcac aaaaaccaga caggtcggaa
     6481 attcttaatt ggctcgataa gttcctcgct ttcaatactt acgaaatatc tatacctgca
     6541 ctcaaagatc ccgatcttgc tccggaagga aaaacaggtc tcataataag ctttcttgcc
     6601 gactacgatc tttttaagaa agtccacgac gaaggctggc ataaagaatt tattactgaa
     6661 cttgaggagc gcatcatcag agtactttca gattctgtat atcctgtact gaaggaaaag
     6721 gttattgaca ggttttcgtt caccccgcta agctacgaaa aacgggttgg ttcgtctgag
     6781 ggtgcaatta ccggatggtc tttctgcgaa cccatccctg taatcaataa aataactgca
     6841 gccggcaagg cagttttaac tccggtacct tcaatatacc aggcaggaca gtggacatac
     6901 agcccggccg gagtgccaat gtctatcctc acaggcaagc ttgctgcaca aagaatatta
     6961 agttcctgag aatttcaaat cacacggaat tttcagatga aagtcaaaaa tttgctttga
     7021 gaatatcctg tgtggtcagc aaattcaaca actaaaaaat caaagaaagt agtaaaactg
     7081 caattgccgt gataacaatt ccggctacga cttgatttat tattgttctg gcagagaagg
     7141 ttttaaactc gccggagtct gcaatacggt tatacatgct tataagttct tcgcggtcct
     7201 taattttagt gtaacacccc gggctgtcag ctatatagtg ttgtgcataa ggaaaacaga
     7261 ttgctgcact gatttcctgc cgtgccaatc ccgggaaaag ggtattttct ttaatatgaa
     7321 tatcctccac tggaatatcc agggcttcct ttacttccgc cggatcaaag atcactgcaa
     7381 aaggaatatc ttcccctttt acatagctct tacagtgacc tatcgatgta tttcccatta
     7441 accagagctg ctgatcaagg attcgaattt tacctctttt tatgattcct ttaaattgaa
     7501 gttttctctt tgtccttttt tcctcgatgt caaagaagac ctctccctgt gaacttgtag
     7561 ttaaaattgc cctttcacag tttggatcag caatgccttt tgtcggcagt gctataatgg
     7621 tgttggataa aatatctatg aaaataaaag acaacaatcc tgtttcttta tcttttgcaa
     7681 tcaggtaggc ctccattctt gttccccaga atgtgcttgc acgcgtactt agattaccaa
     7741 tacccatgta ataatcgggc acgtcatcag caaacaatct tgtttttgaa agttcatacc
     7801 taccaggaag catcgatttt gcaagttcaa tatcggccag tttaaaaaac agaaaaagac
     7861 tatagggatc aattataaag cctatataag gagtattttt ggaagccctg tgaagcataa
     7921 actttaaaac agccgtgggc aataatcctt ttagcttctg gaagaaaagg agattgctga
     7981 cttccatagg attgatcagg ctttcaaccc ttttaataaa tttttcatcg ttttgaattt
     8041 ccatacgtaa gtttgaaaca aaatatcaag aactgtgcat aaatctaagt tatatatttc
     8101 acatttgaaa acgcaggcag tgcattaaaa atagtctatg aacctttcat taaaaatttt
     8161 ttatataatc gattaaaaat gatcaatttt gaatatatat tgttaaccaa tttgttaaga
     8221 atcttttata gcaattattc tttgatttaa tagacctttt atgaaaatcc ttcttaccgg
     8281 tgcgaccgga tatattgccc aaagattatt accggtacta ttaagaaacg ggcatgaggt
     8341 tatctgttgt gtcagggaca ggatcaggtt taacgataaa tacaattctc ctttattaaa
     8401 tgtaattgaa gcagattttt taaagaagga gactcttact gtaatcccaa aggatataga
     8461 tgcagcctat tatttaatac attcgatgtc tttatcaaaa ggtgattttg aaatcctcga
     8521 aagtatatct gcccaaaatt ttaaggacat tattcagcag acaagtgcaa ggcaggtaat
     8581 ataccttagc gggatagtta atgaagcaag gttatcaaag catctttcaa gccgcaagaa
     8641 tgttgaggcg attctgacgg gaggtagtta ttctcttact accctgcgtg caggtatcat
     8701 tattggatca gggagcgctt catttgaaat aataagggat cttgtcgaaa aactcccttt
     8761 aatgatagca ccgaaatggt taaacacctt atcccagccc attgccataa gaaatgtcat
     8821 tgaattccta tcaggcgtat tgttgaatga aagtacattc aatcatagtt atgatattgg
     8881 tggacctgat atcctcacct ataaacagat gctgttgggg tttgcaaagg taaggggact
     8941 cagacgatat ataggaattg tcccggtaat gacgccaaag ctctcatcct actggcttta
     9001 tttcatcaca tccacttctt acaggcttgc acaaaacctg gtcgacagta tgaaagttga
     9061 agttatttgc aaagaaaaca atctcgggga aattttaggg attaaacctg tcgggtatga
     9121 tgaagcggta gctgaagcat tggaaaagat cgaattgaat caggtaatat cgagctggaa
     9181 agatgcattg acatcagata ctcttaacgg aggtattctg aaacatctgg atgtacctgc
     9241 atatggctgc tattttgatt acagaaaacg aaaggtaagg gatcctgaat gtaccttaca
     9301 aaagatctgg tctgttggag gaactgtcgg atggtattac ggcgactggt tatgggcgat
     9361 caggggtttt attgataagt tatttggcgg ggttggactc aggaggggcc gtaaaagcca
     9421 atctgaaatc tcacctggcg aggcattgga tttctggaga gtactggcag ctgacaaaag
     9481 tgagaaaagg cttttattat atgctgaaat gaagttacca ggtgaagcct ggctggaatt
     9541 caaaatagat aaagataata tactgcacca gactgctaca ttcagaccac tcggactaac
     9601 agggagatta tactggtacc ttgtactgcc cctgcatttc tttattttca gaggaatgat
     9661 aaaaaagctg gcatgttaat gtgaacggtt attatccaac aggaatgaat tcgattggaa
     9721 tgggatgtac ctaaactaaa tattggctga catcctgtaa gatagataaa ttttttttca
     9781 actaaccata atagcaaaat atcaattaat aagcgggatt taatctcaaa tcggacaaat
     9841 actacctttg gatgtagatt tacaactata agtagcgaaa taacaactat aagtaattga
     9901 tatattttga ttatggaaat tctccaaaat aactttgccg tatcaaacaa ttttaaaaaa
     9961 taaaagatat ggtaaacttt aaattgattg gcaataagat tgccgaagca cgaaaaaaag
    10021 taaatatttc tcaggcacaa cttgcccagc gacttttcat aagctcacaa gcagtaggaa
    10081 aatgggaacg cggagaatca atgcctgaca tcattacttt taaccgtctt gcagaaattt
    10141 tgggtgttga ccttaactat ttttcagaag gcttattccc tgtcccagca gaaaaagttc
    10201 taattgattc ttcagaaaat aagacaaacg atttactctc cgcaaaaccg aaaattaatc
    10261 gcagctggga tatgtcacgc gggaactgga tggatgctga cttctccgga ttaaagaatc
    10321 tgcacgaaag attcagttca tctaacatgc ttcgctgcaa gtttgttggt tctgatatgt
    10381 caggacttct tctaaaaagc aataacgtgc aaacctgtga cttttcaggt tctgacttca
    10441 gcagaagcca aatccaaaac tccaatttat caaacaacct ttttaaagac tgtccgatga
    10501 aagaaactga attctcacaa accaatatca ttgattgcga ttttaccggt gcagatttta
    10561 caggagtgat aattaaagct ggtggttttg ggaaaaatac aattgtggat gcaatttgga
    10621 attgtacttc ctttgttgat tcacacttag cagatgtaat tttcactggt acattaaagg
    10681 actgctcctt tgaaaactgc tcgtttacca agttgatttt caaaaatgcg actattctaa
    10741 acactttctt taaaaacaaa agtctaaaaa ggatccagtt cattgactgt caggcagacc
    10801 ggataaccta cgaattcctg aaaaacggaa aagcaaattt aaccgggatc actttgatac
    10861 caaaaggaga tgaaaaataa aattgtcccg aaatgctaac acagacggta taattaatgg
    10921 gctcgtccac taaattatac cgcctggttc agcgttcatt gccacccaac aacagcatgc
    10981 aaatgaataa aatagcttac tgcatttcaa ttttaattat cggaattgtc gcatatttat
    11041 tccttttcgg aaagcttttt gccttttccc ctataattat cggatttgat aaacatgagt
    11101 caaaaaatgc aatattttat attcaaaaag attcaaagta caatgatttt gaattaattg
    11161 atacattaat tccttctgtc gaaaattttc acgaattaaa ctttatcagg aagcccagga
    11221 tttttacttt tcaagatagc ttgacatata ttcaccattc actttctaag gccagattct
    11281 gtgctttttc aagtggcagg ttatttattt ctccatgggc attaaaagag gcaaaacaag
    11341 gaaagatctc aataccaatc tacacaaaac atgaactttc gcatgtctta attttacaaa
    11401 ataaaggtat attttcggaa ttaaaattcc cagaatggtt acttgaagga atcgcagttt
    11461 atagtgcaaa tcaattggga acttcattct atcctggtaa agaagaaaca tacaaggcga
    11521 ttgcccaggg gaatttcatg cctccttttg atttcaaaac aaaaagagaa agcaaaatca
    11581 atctagatgt aaaataccgg atcacattca tgtattcaga gtttgcttgt attgtagatt
    11641 atcttatcaa aaagtatgga aaagataaat tcctgtccta catgaaagct ttaatcaagg
    11701 aaaatgacca tgacgaaatt tttaagaaaa tatatggagt tgactttgat gaatcaatag
    11761 aagaattttt gagatatgta accacaggta tataaaatta ctgaaataca ttgagtttca
    11821 taaatgcaaa ataataatat ttgtaagatt aaaatcagta aatttaaact ccgaaatact
    11881 tttaatataa cctgaaaaaa atagattatg atcaacagaa aattgattac cagctttttc
    11941 ctgattgtgg caatagttgt ccctgcatac ggacaggcca atactcaaaa agcattaaaa
    12001 gatgcatacg cagggaaatt cctgatagga gctgcaaatg acctgacaaa aatctctgat
    12061 gctgaagcgg caaatattaa aatgcattac gacatcatca ccccggaaaa ctgcatgaaa
    12121 ccacaaccaa ttcatccttc ggaggataag tacaactttg taaccagcga tgcaatggtt
    12181 gagtggtgtc agaagaatgg tttaaaggtt tggggacata ccttagcctg gcattctcaa
    12241 tctccggcat ggttctttca ggaaaagaat gcagcgacag gagaagcagc tgctcctacc
    12301 cctcaacctg caactacgcc tgcagcagca ccggatccta atggtccacg tcccggcatg
    12361 ggaggttttg gtgatcgcgc taacactggc ccccaggcaa gccgggaagt catcctggaa
    12421 cgtctgaaga atcatattat gactgttgtt gggcgataca aaggaaaaat aactggatgg
    12481 gatgtattta atgaatcaat tgctgacggt ggtgacggta aaacagaaaa tctccggacc
    12541 tccagctggt ataaggccgt cgggcccgat gttattacac tggctttcaa gtgggcacat
    12601 gaagccgatc caaatgctca gctatactac aacgactaca acatcgagca gggcgctgtt
    12661 aaaaacacag ggaaacacgc cagttccatt ttgcttctaa agcggctcat tgcagaaggt
    12721 gccccgatca ccggcgtggg aattcaggga cattggcacc tggacactaa ccttgaagac
    12781 gttgaaaaag ctatcgagaa ttacgcttcc ctgggcttga aagtatctat tacagaactg
    12841 gacgtgacag caacaggtac aaatagtggc gcttttggcg cagggcaggg aaatagaaca
    12901 atcccgcccg agaactacca gaagcaggct gaggtataca aaaagctgtt cgaaattttc
    12961 atgcgtcatg cagatgtcat cgaccgcgta accttctggg gtcttagcga tacaaggtcg
    13021 tggcgcaggg gacaggatgc acttttgttt gatggccagt tgaatcccaa accggcattc
    13081 aatgctgtta ttgaagcaag ctcgtctaag taaattctgt tctgtcagaa tactaaggtt
    13141 tacaaatgct ctcaattaat gggcttccct ccgaaacggg ccaattaccc gcatgtttcg
    13201 caggggagct ctttcttttg taccacgctg ttataattga tattcattta cagtcactta
    13261 gattaataac caacataaca tgaaatcgat tatagcagga ataaatctat tgattctaat
    13321 actaatagtg agttgcggca gagaccttga ccaatcaaaa agatattctg atatgaaatc
    13381 ggaatatgat agtcttattg ctcaggccaa tttgattatt aatgagcaaa agactattgc
    13441 tgatagctta aaaaatatca gggacgatat agtaaggaac aaggcactga attataaatt
    13501 accagaaata aacaggaaaa ttacgaatgc aagagaaatt gagtccaata ttgaggagat
    13561 aagaaaacat ctggaggcaa taagactctt tgcaaacaat ggcgagaaag taactaaaag
    13621 aatcatatac tttaaaagaa aatatatagt ctattcaatt actattcaga tacatgacga
    13681 aaatattatg atccgggata taacaggtga tgtatcagca aagcaatact tatcaaggta
    13741 tttatctagt gaaagaaaat aggtacacaa cctgatacga ataattaagc tcatcttata
    13801 tcatactttt tgtttccttc taccaataat aaaccgtttg cagagagaag aaaccagtcg
    13861 atgcaatcga gaaggaaggg aggaaaatat ttttgcttgc tgtagtattt taaattgatt
    13921 tgtttacttt tacactttag cggccggagc aaagagtttt ttatctacag aatcctaagt
    13981 cattacttta ttccatgact atcagatatt tattgcaaca tatctttata tttaaatcac
    14041 tacctgaaac ttaaattttt taaaatgaga agactgtcat ttctatttct tgtattgttg
    14101 ctggcagtag catgcaataa gggtccgaac caggattcga accaggattt gaaactcaat
    14161 gatcttgggt atttcgagac gcaaggtgta aatgtccttg tgtataataa tctctttacc
    14221 ggaggtttta acgatgaaaa gactgccggt atagaactga ttcaccatgg agtcagaact
    14281 gcacagggag gtgctgtaag actttccaac actccggagc agtgggacct tgtcccggca
    14341 ataccaatcc gaacagtcga caccctggcg aataaaattg agtccacctt aaggtatgaa
    14401 gattacgatt ttgattcgcg ggttattgtc tcagccaggg gaaaaggtgt tgagatatct
    14461 gtttaccttg ataaaccagt tccggctgca cttgaaggcg ctgccggatt caatcttgag
    14521 ttccttccct ctcagtactg gaataagacc tttctgatgg atgggcgtcc caatcgattc
    14581 ccgcgctatg ttgtaggcaa cacaattaca aggcccaaca gtgaaaaaat caagcaattc
    14641 aaagggtata aaacttatga cgacagggga accggcaaat ttatcgatcc tcttccactt
    14701 gaaacaggcc gtacaatcct catggcacct gaagaccccg aacgtttggt aaaaataacc
    14761 tccgaagatg ccgatcttat gcttttcgac ggccgtattc tggcacaaaa cggatggttc
    14821 gtagttcgca gtttacttcc ggcaggaaaa acaggcaagg ttctgacatg gacagttgaa
    14881 cccaatgcaa ttaaaggttg gataagagag ccaaatattg gtttctccca ggtgggctat
    14941 ataccttcgc aaccaaaagt ctctgtaatt gaactcgata agaaagacaa accgctcgca
    15001 aaagcatcga tatataaagt aggcgacgat ggcaatgcta ctgaagtatt cagtggtaaa
    15061 atcgtaccct ggggtgacta ttttaaatat cattatgtaa aattcgattt ttcttcagtc
    15121 aatacccccg gcatatacta catacagtat ggtgacatta aaacaaataa ctttttaata
    15181 gaaaataacg tttacgacaa aatgaccgac gccacgagcg atatatggat cccgatacat
    15241 atgaatcata tgttcgtaaa cgaggcttac agggtttggc acggtgagcc tttcaaggag
    15301 ggctatctac aggctccggc acccaccgat catttcgaca tgcaccgcca gggaccgacc
    15361 accgacacaa agtataaagg gttggaattg atccccggat taaacatagg aggtttcttc
    15421 gatgccggag attttgatat cgagacaggg tcgaatattg gtgtggtgca gaactttgtt
    15481 cagacatggg aatatttcaa acccctgcga gatcagactt ttgttgatca gaaacagcgt
    15541 tatgttgatc ttcaccgccc ggacgggaca ccagacatcc tgcagtttat cgagcatggt
    15601 acattgaacc tgttagcaca ggcagagata atcggacaca tggcatcaac tctatctaac
    15661 tctgttctag ataattacca tcatcttgga gatgcagcct caataacaga cggccttccc
    15721 tataatccta atcttggtcc ttacgaagta gcccgtgatg gccggtcgag cgggcttaag
    15781 gatgatatgt gggcatttac aagccgcaat ccaggcaccg atctcagggc agcaactatg
    15841 tttgctgcag caagccgggc gctaaaagga tataacgaca atctttcagc aagagcgttg
    15901 aaacagtcaa aaaggcttct gaaagaggca acggaattgc ttgccaaaca gccacagggt
    15961 cctgccggca gaagcttcgg atcagcagac atgggcacca atcttcaact ctacatctca
    16021 accggggagc aacaatatat cgataaattt caggagcttt tatggcccgc acttgagcgt
    16081 aacgttagtg gcagcatatt gacagcattg catgctatac cgcacatgga cgcatcctat
    16141 aaagaaaagc tgcggcccta tgttgtaaag tacaaagaat atgtcgacga gatggaacag
    16201 gataatccgt acggagtacc catcggcctt ggcaactggg caggaagcgg aggcgttgtg
    16261 agctttggta caactatttg ctttgctaac atgcattttc ctgatatcat tgatgccagt
    16321 catgctttca agactactaa ttggctgttt ggctgtcatc cctatcataa ttattccctg
    16381 gtggctacat tgggcgctac tcgtcccaaa gctgtcttct atggaaataa cagggctgat
    16441 ttctcattta ttccggggaa tgttgctcct ggtatattat tcaggcaacc cgaccatttt
    16501 gaaaactacg acgactggcc atttctttgg ggccagaacg aaggaacaat tggtggaaat
    16561 acaagctatt taatttttgg atctgctttt aagaatatgt taaaataaag atcccgcttt
    16621 tgttagcatt ataaaaagct ttctccatat ctactcggtt actatttact ggaaaaacag
    16681 tttagagtga taattctttt tgtagatttg ctaaataagt agtcatattt ttcgtagcca
    16741 taatcgataa ccctgtaaaa atttactatg aacattaaac caaaaacatt cttatcggcg
    16801 gtaatcactg taattctggc agtatccgga ttacagggat acgctgctat gtcaggcgac
    16861 aaaactgata gcgctcaaca aagcgataat aaacgcagta tcgctcccta cttcattcgg
    16921 gccactaaag cgaaaaagac accggataca gatggattca ttcaacgctg gttactactg
    16981 gagccaatta acaaaccaaa ccgcagcaat actgttttta cagacagcta cataagaaca
    17041 gccttcacaa ccgagtattt cccaaaccag tttaccggta ttcccaa
//
LOCUS       DAOSNB010000018        16994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3395_length_16994_cov_4.803235, whole genome shotgun sequence.
ACCESSION   DAOSNB010000018 DAOSNB010000000
VERSION     DAOSNB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16994
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_16994_cov_4.803235"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..666
                     /locus_tag="PLG42_01705"
     CDS             298..666
                     /locus_tag="PLG42_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29957.1"
                     /translation="MYRNLFRFAVMTVTILAANLITSTLNNYLITFRHHVRPVTFTII
                     GMIVTVLIFYPLFVKLEEWVENISTDILKRGRSMTGKYLGLIFSFFVCLIVLAWFYAK
                     MWYRIDLLKILIRGDLGRYV"
     gene            complement(724..1068)
                     /locus_tag="PLG42_01710"
     CDS             complement(724..1068)
                     /locus_tag="PLG42_01710"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HOU29958.1"
                     /translation="MKVDRLEFGSITIDGKVYKEDVVIDNGVIRKRKKSKSKKYRDKF
                     GHTPLSSDEKIPWKCRHLIVGTGHSSSLPVMNEVFKKARKKGVELHLMTTPEAIKHIN
                     DPDTNLILHLTC"
     gene            1187..4513
                     /locus_tag="PLG42_01715"
     CDS             1187..4513
                     /locus_tag="PLG42_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU29959.1"
                     /translation="MYTLKQLITTGLLFFSIAVKILSQDLDSAMKFFDGAKYEKAAEE
                     FEKVLPLLEKIYGENDTSYYTIFLMYAAFSNEKCNQFEKAENYYLKIREIYRNLKDTS
                     AYLYVTATSNLAELFRITGKFKSAEAIFKEAIILNSKIYGIESLQYMNMLNNLALFYR
                     ETGRYKEAEEFFIRAIDICRKLFSEDHPRYMILTGNLAALYHKMGNYVKAEPLYFKVL
                     QFARNYYESGSPEIVEILNNLGAFYEDTGNYEKALELYTESLNIRAKTFGSGHPEYAI
                     AANNLAQLYFKTGFFEKAEQLYYKALEIYGNTYGENHMLYASALNNLGVLYHNSGNFS
                     KAEKIYSEVLKIRKNILGEKHPDYANTLGNLALLYHELGNYERAETLYYQALKVREET
                     TGESHPDYSVTLNNLALLYCDMSNFSKATVLFQKAAEIRENISGKYHPAYAVVLNNLA
                     LTYACSGNYDRAEKTLSEVIEIYEKSSGNIHPDYATALSNLADIFWSKGNYEQSARLY
                     LQALDIFTETAGQMHPGRTSVLNNLALLYHETGNINEASKYYHLAKEVYMKQIRQQFG
                     FLSSSEKEKYITSIQHFFKTYQNFIFKALNESPFLATEAYDIELAWKSLILHADKQMR
                     MAVLESGDSGIIKKFDSWLAAKASLARQYSLPLSKRNTDVQLLEEQAENLEKELTRFF
                     SDKGILRSIRDVKWQDIRRCLQPDEAAIEYTYFPWYNGRKWTDSIYYCAIVVRNNFSY
                     PIFILLFEEKQLDSLLVKAKGSDESYIDDLYAWNDYYNPGTPGKGKQIFELLWKPVEK
                     YLEGVKRIYYSPTGRLHQLSFSAIPVTENEILSDIYDLHQMISTAEITENIPDKPSGN
                     IVLYGGINYGQQPSVDMNSGHEKRGNYLMFLDGTLTEIQKIKKIAEDKGFKTIVFSGD
                     NATEPSLRNLSIKERPGIFHFATHGFFLQKADSSITNLRHTVRGYYGSLQTHNISENP
                     LMRSGLALTGANMAWGSGEIFPDSDDGILTAYEVSNLYFPGTILAVLSACETGLGEIK
                     GSEGVYGLQRSFKIAGVNYIIMSLWKVPDYQTSELMTLFYSEWFSGKSVKDAFSIAQS
                     SLRKRYPRMPFMWAGFVIVR"
     gene            4588..5955
                     /locus_tag="PLG42_01720"
     CDS             4588..5955
                     /locus_tag="PLG42_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU29960.1"
                     /translation="MRSRKKFLSRRQFISATAGALAAPYIISCSSKPKRTDNKLHHAC
                     IGVGGMGWQDLQRFLEDPNVKIVAICDVDTTFLKKASEAVPEARVYTDWRELLKKECD
                     NIDSVNVTVPDHNHFPIAYNFIKKGKHVYLQKPMCHDVAEVRILTEAAIKAGVVTQLG
                     TQYASLKGDRTAVRLLKDKAIGKIKHAYLCSNRPGAIENYRLAGPRPEKGTEPPANLN
                     WDLWIGTAPERPYAPDIYHPVKWRAWQDFGTGWSGDIGCHIFDAVWKGLSMKQPLSVI
                     AEVQKSWKESPARRADNWPQGDHITWIFPGNEYTESDTLTVEWFDGEFYPPEEIRALF
                     PGEYPAESAMLIGTEGALLIPHGRMPVLLPEDKFKDYKMPVFESGNHYNSFVIASLGG
                     PPTESHFAQTGPMTEAILLGTIAIRVPDILLEWDPVKMRFPNYPDANKFLRRRYRKGW
                     NTGGF"
     gene            complement(5995..6558)
                     /locus_tag="PLG42_01725"
     CDS             complement(5995..6558)
                     /locus_tag="PLG42_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU29961.1"
                     /translation="MKNRLISLIFFIIISFASALNGQQAVKSATEVILSGYSVRMFAP
                     GTVSDSDIELILKCGIKAPSARNLQPWWFTVVKDQALLKELMPNITEGNILIIISGRE
                     DARWAEYDCGLATQNMYIAAQSLGLGAHIYAGPVESINTSKKEVLGIPSGYRAIMVLR
                     IGHIDKNVDAVSAASPRKSIQETVNFK"
     gene            6670..9873
                     /gene="carB"
                     /locus_tag="PLG42_01730"
     CDS             6670..9873
                     /gene="carB"
                     /locus_tag="PLG42_01730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOU29962.1"
                     /translation="MPKRTDIEKVLIIGSGPIVIGQACEFDYSGTQACKALRSLGYKI
                     VLVNSNPATIMTDPEMADVTYIEPLNEYALTEIIKKERPDALLPNLGGQTGLNLSSQL
                     AKSGVLDKYNVKVIGVNINAIERGEDRIAFKETMKKLGIEVPRSKAVNSVKAAEDVAA
                     ELGYPVVIRPAYTLGGTGGGLVYNVEELRNVAGRGLAASMVHQILIEESVLGWEELEL
                     EVVRDSKNQMITVCFIENVDAMGVHTGDSFCTAPMLTIPPELQKKLQKYSYQIVEAIE
                     VIGGTNIQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAYISALLACGITLDEL
                     PYWREGTLDKYTPSGDYVVVKFARWAFEKFEGLEDKLGTQMQAVGEVMSIGKTYKEAL
                     QKAIRSLEIGRYGLGFAKDFNSKSLDELMALLKHPSSERQFIMYEALRKGADIEELYK
                     RTHIKPWFIQQMKELVEEEEKILSYKGKTLPDELLVQAKKDGFSDKYLSFLLNIPEKE
                     IRERRISLGVKQAWEAIPVSGVENAAYYYSTYNAPDTVKVSNKKKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFAIRDAGYESIMVNCNPETVSTDYDTSDKLYFEPLTLEDVLSIYNK
                     EKPEGVIVQFGGQTPLNLANDLAEAGVKILGTSPDTIDLAEDRDRFRKIIKKLGIPQP
                     ESGMASNLEEAIAVAEKIGYPVMIRPSYVLGGRAMKIILDREMLEQYVNEAVGVTPDR
                     PLLIDLFLEDAIECEADALSDGTESFVPVIMQHIEYAGIHSGDSACVIPPVIIPKEHK
                     QTIYDYTRKIAKELNVIGLLNIQYAIYDNVVYILEANPRASRTVPLVSKVCNIKMAWI
                     ATQIMLGKKLSEFNLKNKNLKHYGVKEAVFPFNMFPTIDPLLGPEMKSTGEVLGLADT
                     YGMAFFKSQEATQSPLPIKGTVLITIADRDKEKILPAAKNFRDLGFTILATRGTKNFL
                     ESHGIPAKLVLKVHEGRPNIVDAIKNGEIDLLVNTPAGRLSEYDDSYIRKNAIRYKIP
                     YITTTSAALSATNGIRERQNGQYKVKSLQEYHSEIED"
     gene            10052..11446
                     /locus_tag="PLG42_01735"
     CDS             10052..11446
                     /locus_tag="PLG42_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOU29963.1"
                     /translation="MKKIVLAALITFFCNYSLSAGYRAGVAKKIITPETPVQLSGYAS
                     ASRAQPVGEVLHDLWAKALVLSEDNREGSMIIIVTMDVIGLSHELSESIIERVTSQYK
                     IDRSQLLINASHTHSGPVIWPSLMNMFDLSKEDIQSLVKYNKRLTEDVLELIGMAISD
                     LRPVKISIAHSSAKFAVNRREKTDRGVVIGVNKNGPVDYDVPVLKIETYDSRLFAVLF
                     GYACHNTTLDINQINGDYAGFAQIEIEKNNPGTVAMFLSGCGADQNPYPRRSVEAAIQ
                     HGKDLAEAVQHALKGDFKPVGTPLRTFFTTCELEFAPFVPEQFREEMLGNDRFRFKRG
                     KFILEAMDKGYNVQTIRYPVQAVRFGNDFTIIALAGEVVVDYSLKTKKLYPDEDIFVA
                     GYCTEVQCYIPTAEILKEGGYEPVTSMIYYGLPGPFSENVENKIFTAINLAMKKTGAK
                     AYTRKKLNLLMNNL"
     gene            11585..13042
                     /locus_tag="PLG42_01740"
     CDS             11585..13042
                     /locus_tag="PLG42_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU29964.1"
                     /translation="MKKTSGGGLNRREFLGLSALGLTGLTILPSYVIDGMRIPPSDRI
                     LMGTIGLGRQSLSDFRGFAGYPGLQIVACCDVDTNKQLRYKRTIEEWQKSKGLAPRCD
                     VYEQYERLLERKDIDVVEIVSPDHWHALMTIHACQAGKDVYVQKPLSYTIKEALVMTR
                     VANDTGRVVQVGSQQRSSPEFQKAIELVRAGAIGHIEKIYAKVGDPPKPFDLPEQPIP
                     GTINWNLWLGPLNDEKIVYHPDLAPPISLDPPVNESIWGAWRWYRETGNGYTADWGAH
                     MFDIAQAAIGMDGSGPYEVIPKGYNGQPYLTFKYLTGVVMTEQPYLEDNPTAQGIKFI
                     GTKGWIEVARGYIACSDSSLIPPEIAGRRPQPRTAQAGGQQGQRQTQQAQGQQQRQQQ
                     QQARPAPTQEGLRFEISSPHMTNFLDCVKSRKKPIASIEVGCSTAITCCLGNIATELG
                     RPVKWDPATLTFVNDPEAENHRLMHYQYRRPYKLC"
     gene            complement(13161..14423)
                     /locus_tag="PLG42_01745"
     CDS             complement(13161..14423)
                     /locus_tag="PLG42_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU29965.1"
                     /translation="MRINITSALKEILIAVFILALLVSGGECKAQFPGINDSIAKYRQ
                     GIIVVKAKPGSSFKIEQLRHEFWFGCAISNGLAGNSWSEETKRIYKEKFLENFNAAVT
                     ENALKWANMEPRRGDVNYTVVDMILGWCEANGIPLRGHNLFWGIPQFIQPWVKELKDD
                     ELKQVIKNRAETIAAKYKGRFAEYDLNNEMIHGNYYEERLGPEITKLMAQWVHNGDPD
                     AKLWLNDYDILTGVKLVDYMSNIRLLLRQGVPISGIGVQGHLHAETFDRNQLQRALDS
                     LAIFGLPIRITEFNIPGQRSKYYREKINTLTPEEEELKAKELVDYYRICFAYPAVEGI
                     LMWGFWEGANWIPVSSLYRRDWTPTPAAEAYRNLIFREWWTNITGKIGRDGSFSTKAF
                     YGKYRVTVNGSSREIELRKSEGMTTVVF"
     gene            14713..16845
                     /locus_tag="PLG42_01750"
     CDS             14713..16845
                     /locus_tag="PLG42_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOU29966.1"
                     /translation="MKKSTFISVFCFVFITSTNISFPQFDRRAEELMVLLKSGKQRNL
                     LSIARRVDSVNYRDETDRTLLMYASGLGNTELCKILVEKGADVNLTTRDGVTALMFAA
                     SYGHALTAKFLVENGARLDIRSENGFTALAIASKNGHTEVVKTLLDNGDSPDTRITEG
                     YTSLMIAAENGFQEITKLLLEKGANPDLRTTDGYTALILASQNGNIGVVKTLIDYKAN
                     LDLQTDDGITALIMATRYGYSDIVKLLAYAGANPSLQCADGETALFIAAMNGYTEIVK
                     TLLEKGADVCLPCNDSATPLFIASLKGYTEIASMIIENEKARGVSCVDEIKTLMMTAN
                     DEMAEKVKSILEKRSKSKTEFAKGIGAKVYTTSNGHIEVVEFMLDKEPDPEIEKSGKR
                     RTSLMRASQSGTVELVKMLIKDNIKKDYPFDEKYSALMVACMNGHAEIIKMLLDKGSD
                     VNYRLSDGTTALIYAVQNGNSEIVNMLLQSGADINNICRDGSTALIHASSKGKTEVVK
                     LLLERGARHDLKRNDGSTALTIASGEGFRDIVSLLLDKGADPEVRERNGYTPFMLAVK
                     NGYTDIVKLFLDRISNPDSHTNDGVTPLMQACIGGYTEIARMLLGKGSEINARTPDGY
                     TPLMLAALNGHIETVKFLIDRGAEINQQMTKGTTALMLAASNMHLDVVRLLLDKGAKP
                     DIKDNDGKTAIDYTESFAIKNLIKKYTK"
BASE COUNT         5507 a         3409 c         3328 g         4750 t
ORIGIN      
        1 ttttaatttt ttgacaatct gtaatatata aaatcagata cttaaaaaac ctgttttaat
       61 cctgttgaat gtcgttacat tcgggccttc ttaattttca tgcaaaagga ataagaacat
      121 ttttcacaaa gcctgtataa ataccttcaa tattactagc ttcctttgcc agtttcagaa
      181 tatagccttt tatttcttct ggagtagaca tcgcatttaa atttacatga attgcgaaaa
      241 ttaaaagtat aaaaaattct ttactttttc tgtttgccta atattaaaga gcccgctatg
      301 taccgtaatc tattccgttt cgctgtaatg acagtaacta ttctggcagc taatcttatc
      361 acctcaacac tgaataatta cctgattaca ttcaggcatc acgttcggcc tgttacattt
      421 accataattg gtatgattgt gactgtgctg attttctacc ctctttttgt gaagctcgaa
      481 gaatgggttg aaaatatttc aactgatatc ttaaagcgtg gccgcagtat gactgggaaa
      541 tatttaggac tcatcttctc attttttgtc tgcctaatcg ttctggcatg gttttatgca
      601 aagatgtggt atcgaatcga ccttctgaaa atacttatca ggggagacct gggacggtat
      661 gtatagatat acagggatga gggatgagga atgagtttta aaaattgaat aattaagttt
      721 actttagcag gtaaggtgca ggataagatt tgtatctgga tcatttatgt gttttattgc
      781 ttcaggtgtg gtcattaaat gaagttcaac tccttttttc cttgcttttt taaaaacttc
      841 attcatcact ggaagacttg aactgtgccc tgtacctact atgagatgcc tgcatttcca
      901 tggtatcttt tcatcagagg aaagtggtgt atgcccgaat ttgtcgcggt atttcttcga
      961 ttttgacttt tttcttttcc ttataactcc attgtcaata acaacatctt ccttataaac
     1021 ctttccgtca atcgttatgg agccgaattc aagtctgtca actttcataa aaaatatttc
     1081 cccctgctgg ttcagtaagg caaaattact aaatgacaat aaaataaata cgcatcagga
     1141 ataaaagatc agctaattat tagtaaatta gcttataaag tatcctatgt atactttgaa
     1201 acaactgatt acaacaggcc tactcttctt cagtattgct gttaaaatcc tttcacagga
     1261 tctcgacagt gcaatgaaat ttttcgacgg ggcaaaatat gaaaaggcag ctgaagagtt
     1321 tgaaaaggtt ttacctctgc ttgaaaagat ttatggtgaa aacgacacaa gttattatac
     1381 aattttcctt atgtatgctg ctttttcaaa tgaaaaatgc aatcaattcg aaaaagctga
     1441 aaattattat cttaaaatca gagaaatata tcggaatctc aaagacacct ctgcatatct
     1501 atacgttaca gctaccagca atcttgctga actgtttaga ataaccggga aattcaagtc
     1561 tgctgaagct atatttaaag aggctattat tcttaactca aaaatttacg gaattgaatc
     1621 tcttcagtac atgaatatgt taaacaacct tgcattattc tatagagaaa caggaagata
     1681 taaagaagcg gaagaattct tcatcagagc tatcgatata tgcagaaaac ttttcagcga
     1741 ggatcatccc cgttatatga ttcttacggg aaaccttgca gctctttatc acaagatggg
     1801 aaattacgta aaagcagaac cactttattt taaggttctg cagtttgcac gcaattatta
     1861 cgaatcgggt tcacctgaaa tagttgaaat cctcaataat cttggtgcat tctatgaaga
     1921 taccggtaat tatgaaaaag ctcttgaatt atacaccgaa tcacttaata ttagggcaaa
     1981 aactttcggc tcagggcatc ctgaatatgc tattgcggca aataacctgg cacaactata
     2041 cttcaaaaca ggctttttcg aaaaagcaga acaactatac tacaaggctc tggaaatata
     2101 tggaaacacc tacggtgaaa atcacatgct ttatgcttca gcactgaata atcttggggt
     2161 tttatatcat aattccggta atttcagcaa agctgaaaaa atctattccg aagtacttaa
     2221 gataagaaag aatatccttg gggaaaaaca tcctgattat gcgaacactc tgggcaacct
     2281 ggcccttctg tatcatgaac tcggcaatta tgaaagagct gaaacattat attatcaggc
     2341 attgaaggta agagaagaaa ctaccggaga atcacatccc gactattctg tgacattgaa
     2401 taaccttgcc cttctatatt gtgacatgtc taatttttca aaagcaactg tattgtttca
     2461 aaaggcggca gaaatcagag aaaatatatc gggcaaatac caccctgctt atgctgttgt
     2521 gctcaataat ctcgctctta catatgcctg tagcggcaat tacgacagag ctgaaaaaac
     2581 attatcggaa gtaatcgaaa tttatgaaaa aagctctggg aacatccatc ccgattacgc
     2641 aactgcactc agtaacctgg ctgatatttt ttggagcaag ggaaattatg agcaatctgc
     2701 cagactctat ttgcaggcac ttgatatttt tacagaaacc gccgggcaaa tgcatcctgg
     2761 aagaacttct gtactcaaca atcttgctct tctttatcat gaaaccggca atatcaatga
     2821 ggcaagtaaa tattaccatc ttgccaaaga agtatatatg aagcaaatcc gccagcaatt
     2881 cggctttctt tccagctctg aaaaagaaaa atatattact tccattcagc atttctttaa
     2941 aacttaccag aatttcattt ttaaagcttt aaatgaaagt ccattccttg caacagaagc
     3001 ttatgacata gaattagcct ggaaaagcct gattctgcat gcagataaac agatgcgaat
     3061 ggcagttcta gaaagcggag attcaggtat aataaaaaaa tttgactcct ggttagctgc
     3121 taaagcatct cttgcacgtc aatattcatt gcctttgtca aaaagaaata cagatgtaca
     3181 actacttgaa gagcaagcag aaaaccttga aaaagaacta actcgttttt tttcagataa
     3241 aggtattctg agaagcatca gggatgtgaa atggcaggat atccgaagat gtcttcaacc
     3301 tgatgaggct gcaatagaat atacttattt cccatggtat aacggcagga aatggactga
     3361 tagcatttat tattgtgcga ttgtcgtaag aaacaatttt tcttacccaa tttttattct
     3421 tctctttgag gaaaaacagc ttgattcact ccttgttaaa gctaaaggtt cggacgaaag
     3481 ttacattgat gacctttatg cctggaacga ttattacaac ccgggcacac cgggcaaagg
     3541 caagcagata tttgaacttt tatggaaacc cgttgaaaaa tatctcgaag gagtaaaaag
     3601 aatttattat tctccaaccg gcagacttca ccagctttca ttttcagcta tacctgtcac
     3661 agaaaatgaa attctttccg acatttatga tctacatcaa atgatctcta cagcagaaat
     3721 cacggaaaat atacccgaca aaccttccgg taacatagtg ctttatgggg ggatcaatta
     3781 tgggcaacag ccttcagtag atatgaattc agggcatgaa aaaagaggaa actatctgat
     3841 gttccttgac ggcactttaa ctgaaattca gaaaattaag aaaattgcag aagataaagg
     3901 ttttaaaaca atagtttttt caggcgacaa tgccaccgaa ccctcactca gaaatctttc
     3961 tataaaagag cggcccggaa tcttccattt tgcaacacat ggtttctttc tgcaaaaggc
     4021 cgactcatca ataacgaact taagacatac tgttcggggc tactacgggt ctctacaaac
     4081 gcataatatt tctgagaatc cacttatgcg atcaggcctg gctcttacag gtgcaaacat
     4141 ggcatggggg agtggagaaa tttttcccga ttctgacgat ggtattctta cagcttatga
     4201 agtatcaaat ctttatttcc ctggtacaat acttgcagtt ctgtcagctt gtgaaacagg
     4261 cctgggtgaa atcaaaggca gcgaaggggt ctatgggctg cagaggtcat ttaaaatagc
     4321 cggtgtaaat tacattatta tgagtttatg gaaagtgcct gattaccaaa catctgaact
     4381 tatgacactt ttctattcag aatggttttc aggcaaatct gtaaaggatg ctttttctat
     4441 agcccaaagc tctttgagaa aacgttatcc acgaatgcct tttatgtggg cgggatttgt
     4501 aatagtcaga tagaagtaaa ttaaaagatt ttcataataa agattattta aaataattcg
     4561 taatttgata atcaaaattc atactctatg agatcccgta aaaaattcct gagccgccga
     4621 cagtttattt cagcaactgc cggtgcactt gctgcacctt acattataag ctgttcttca
     4681 aaaccaaaaa ggacagataa caagctccat catgcctgta taggagtagg aggaatgggc
     4741 tggcaggacc tccaaaggtt tctggaagat ccaaatgtga aaatagttgc aatctgtgat
     4801 gtggatacaa catttctgaa aaaggcatcc gaagcagtac ctgaagcccg tgtatatacc
     4861 gactggcgtg agcttcttaa aaaggaatgt gataatatcg attcagtgaa tgttaccgtg
     4921 cctgaccata accacttccc gattgcctac aacttcataa aaaaggggaa acatgtctat
     4981 ttacaaaaac caatgtgtca cgacgttgct gaagtaagga tactgactga agctgcaatt
     5041 aaagctggcg tggtaacaca actaggtacg cagtacgctt cgctaaaagg tgatcgaact
     5101 gcagtgaggc ttctcaaaga taaagccatc ggaaaaatca aacatgctta tctctgttca
     5161 aaccgtcccg gtgcaataga aaattaccgg cttgcaggtc ctcgaccaga aaaaggtaca
     5221 gaacctccag caaacttaaa ttgggactta tggattggta ctgctcctga aagaccttat
     5281 gcacccgaca tatatcaccc cgtaaaatgg agagcatggc aggatttcgg aacaggatgg
     5341 tcgggtgaca tcggatgcca tatttttgat gccgtatgga aaggattgtc aatgaaacaa
     5401 cctctgtcgg ttattgctga agttcagaaa tcatggaaag aatcacctgc cagaagggct
     5461 gataactggc ctcagggtga ccatattaca tggatattcc ctggcaatga atatacagaa
     5521 tctgatacac taaccgtaga atggttcgac ggagagtttt atccacccga agaaataaga
     5581 gctcttttcc cgggtgaata tccagctgaa tcagccatgc ttattggaac agaaggggct
     5641 cttctgatac cccacggaag aatgcctgtg ctcctgcctg aagataagtt taaagattat
     5701 aaaatgcctg tttttgagtc gggtaatcac tacaacagct ttgtcattgc cagccttgga
     5761 ggacctccta ctgaatcaca tttcgctcag acaggaccaa tgactgaagc tatactgctg
     5821 ggtacgatag caatacgtgt acctgatatc ctgcttgaat gggatccagt aaaaatgaga
     5881 ttccctaatt atcccgatgc aaataagttt ctccgccgca ggtatagaaa aggttggaat
     5941 accggcggtt tctaaaatgc tggaaaaaca aattagatca gaaaatgaaa ccgtttattt
     6001 gaaattcacg gtttcctgta tgcttttcct tggtgatgcc gctgaaacag catcgacatt
     6061 cttgtcaatg tgacctattc taagtaccat tatagcacgg taccctgagg gtataccaag
     6121 cacctccttt ttagaagtat taatactctc cacaggtccg gcataaatat gtgctccaag
     6181 gccaaggctc tgtgcagcaa tatacatatt ctgggttgcc aggccacagt catattcagc
     6241 ccacctggca tcctcccttc cggaaataat tattagaatg ttaccctctg taatgtttgg
     6301 cataagctct ttcagaagag cctgatcctt cacaacagta aaccaccatg gttgaaggtt
     6361 acgtgcactt ggagctttaa taccacactt aagtatcaac tctatgtcac tgtcagaaac
     6421 agtccctgga gcaaacattc tgactgaata gcccgatagt ataacttcag tagcactttt
     6481 aacggcttgc tggccgttta aagctgaagc aaaagaaatt attataaaaa aaattaaact
     6541 gattaaccgg tttttcatag ctgtacttat ttatcttcac aaatttaaat tttttctcca
     6601 taaataattt aaaataactt tttctcaaat tgatttaata aattgcacac tcaaaaaaat
     6661 ttttttatta tgcccaaaag aactgatatt gagaaggttt tgattattgg ctctggtcca
     6721 atcgtaattg gacaggcatg tgagtttgac tattcaggta cccaggcatg caaagcacta
     6781 aggtcactgg gttataaaat agtacttgtc aactctaatc cggccacaat aatgactgac
     6841 cctgaaatgg ccgacgtcac ttatatcgaa cctttgaatg aatacgctct tacagaaata
     6901 attaaaaaag aaaggcccga tgcacttctt ccaaaccttg gaggacagac agggttaaac
     6961 ctttcttcac aacttgcaaa aagcggtgtg ctcgacaaat acaacgtaaa ggttattggc
     7021 gtaaatatca atgcaatcga gaggggcgaa gaccgtatcg cattcaagga gacaatgaag
     7081 aagttgggca tagaagtacc cagaagcaag gctgtaaatt ctgtaaaagc agcggaagac
     7141 gtggcagccg aacttggata ccccgttgtt ataaggccag cctatactct cggaggaact
     7201 ggaggaggtc ttgtttacaa tgtcgaagaa ctaagaaacg ttgcaggcag aggtcttgct
     7261 gcaagtatgg ttcaccagat attgatagaa gagtcggtgc ttggatggga agagcttgaa
     7321 cttgaggtag taagagactc aaaaaatcag atgattacag tatgttttat tgaaaacgtc
     7381 gacgcaatgg gagttcatac cggagattca ttttgtacag ctcccatgct aactatacca
     7441 cctgaactgc aaaaaaagct tcagaaatat tcgtatcaaa tagtagaagc tattgaagtt
     7501 attggaggaa caaatatcca gtttgcacac gatccaaaaa caggaagggt tgtagtaata
     7561 gaaataaatc cacgcacctc acgctcctct gctctagctt caaaagcaac cggtttccct
     7621 atcgcttata tatcagcatt gctggcctgc ggaataacac ttgatgaact accttactgg
     7681 agagagggaa ctctagataa atacacacct tcaggcgact atgtggtagt taagtttgca
     7741 agatgggcat ttgaaaaatt tgaagggctt gaagacaaac ttggtaccca gatgcaggca
     7801 gtaggcgaag ttatgagtat aggaaaaaca tataaggaag cccttcagaa agcaataagg
     7861 tcgcttgaaa ttggaagata cggactcggt tttgccaaag attttaactc aaaatcgctt
     7921 gatgaactga tggctcttct taaacaccca tcaagcgaaa gacagtttat tatgtatgaa
     7981 gccttgagaa aaggagcaga tattgaagaa ttgtacaaaa gaacacatat taaaccctgg
     8041 tttatacaac agatgaaaga gcttgttgag gaagaggaga aaattctgtc atacaaaggc
     8101 aaaacacttc ccgacgagct acttgttcag gcaaaaaaag atggcttctc cgataagtat
     8161 ctctctttcc tgctgaatat ccctgaaaaa gagataaggg agaggcgaat atcgctggga
     8221 gtaaaacagg catgggaagc aattccggta agcggagttg aaaatgctgc atattattat
     8281 tccacatata atgctcccga cactgtaaaa gtaagtaaca aaaaaaagat tatggtcctt
     8341 ggcggaggtc caaacagaat tggccaggga atagaatttg actactgttg tgttcatgca
     8401 gcctttgcaa tacgtgatgc aggatacgaa tcaattatgg taaactgcaa ccctgaaaca
     8461 gtatctaccg attatgacac ttctgacaaa ctttactttg aaccacttac acttgaggat
     8521 gtcctgagta tatacaataa agaaaaacca gaaggtgtaa ttgtccagtt tggcgggcaa
     8581 actcctctta accttgcaaa tgatcttgct gaagctggag taaaaatcct cggaacttcc
     8641 cccgatacca tcgaccttgc cgaagacagg gaccgtttcc gcaaaatcat aaaaaaactg
     8701 ggtatacccc aaccagaatc gggaatggcc agtaacctcg aggaagctat tgcagtggct
     8761 gaaaaaatag gataccctgt aatgattaga ccttcctacg ttctgggcgg ccgtgcaatg
     8821 aagattatcc tcgacagaga aatgcttgaa caatacgtga acgaagctgt aggtgttaca
     8881 cccgaccggc ctcttctcat cgatcttttt ctagaagatg ctatcgaatg cgaagccgat
     8941 gccctctccg acggaactga atcttttgtt ccggttatta tgcagcatat agaatatgcc
     9001 ggtatccatt cgggcgactc agcctgcgta ataccaccag taattatccc gaaagaacat
     9061 aagcaaacta tatacgatta taccagaaaa attgcgaaag aattgaatgt tattggtttg
     9121 cttaatatcc aatatgcaat ctacgataat gtagtatata tccttgaagc aaatcctaga
     9181 gcttcacgta ctgtaccgct ggtgtcaaaa gtatgtaata taaaaatggc atggattgcc
     9241 acccagataa tgcttggcaa aaaactctct gaattcaatc tgaagaataa gaatcttaaa
     9301 cattacggag taaaggaagc tgtttttcct tttaatatgt tcccgacaat tgatcctctg
     9361 cttggaccag aaatgaaatc aacgggtgaa gtactgggac tggcagatac ttacggaatg
     9421 gcatttttca aatcacagga agcaactcaa tcccccctcc ccatcaaagg tacagtgcta
     9481 ataaccattg ccgaccggga taaagaaaaa attctacctg cagcaaaaaa tttcagagat
     9541 ctgggattta ctatattagc aacaagaggc actaagaatt ttcttgaaag ccatggtatt
     9601 cctgctaaac tggtcctgaa agtacacgaa ggaagaccta atatcgttga cgcaatcaag
     9661 aacggagaaa tagatctgct ggtaaatact ccagcaggac gtcttagtga atatgatgac
     9721 tcatacatcc gcaaaaatgc aatccgatat aaaattcctt atataacgac tacatcggcc
     9781 gccctttcag caactaacgg tataagagaa agacaaaacg ggcaatataa agtcaaatcg
     9841 ctgcaggagt accactctga aatagaagac tgaaaaaacc tcagctcata ctcaaacttt
     9901 taccctgatt gaggtatccc ggtttgttgc atactctggt taatagattc ctgtacatct
     9961 tttttatttt ggcatccatc ttcatataga aaaaagcaaa aatagtgctg aaaaataagt
    10021 aaattactgc catttaaaat accctggaaa aatgaaaaag atcgttctgg ctgcattgat
    10081 tacttttttc tgcaattatt ctctttcagc aggatacagg gcgggagtgg caaaaaaaat
    10141 aataacccct gagacacctg tacaactttc aggttatgcc agtgcaagcc gggcacaacc
    10201 tgtaggtgaa gtccttcacg acctttgggc taaagcactt gttttatcag aagacaatag
    10261 agaagggtca atgataatta ttgtaacaat ggatgttatt ggactttcac atgaattgtc
    10321 ggaatcaata attgagagag taacatcaca atacaaaata gatcgctcac agcttctcat
    10381 caatgcttca catactcatt caggcccagt aatatggccc tcgcttatga atatgttcga
    10441 cctgagtaaa gaagatatcc agtctcttgt aaaatacaac aaacgtttaa ccgaagacgt
    10501 cctggaacta atcggcatgg ccatctctga cctcaggccg gttaaaatat caattgcgca
    10561 cagctcagcc aaatttgctg taaacagaag ggaaaaaact gacaggggag tggttatcgg
    10621 agtaaataag aacggcccgg tggattatga tgtacctgtt cttaaaatcg aaacatatga
    10681 tagtaggtta tttgctgttt tattcggtta tgcatgccat aacacaactc tcgatataaa
    10741 ccagattaac ggtgattatg ctggatttgc acaaattgaa attgaaaaaa ataaccctgg
    10801 aactgttgcc atgtttttat caggttgtgg agccgaccag aatccttatc cccgccgttc
    10861 agttgaagca gcaattcaac atgggaaaga cctggcagaa gctgttcaac atgcactcaa
    10921 aggcgatttt aaacctgttg gaactcctct aagaaccttt tttacaacct gtgaacttga
    10981 gtttgcacct tttgtacctg agcaattcag agaagaaatg cttggaaatg accgttttag
    11041 attcaaaaga ggaaaattca ttcttgaagc aatggataaa ggatacaacg tacaaactat
    11101 tagatatcct gttcaggctg ttcgtttcgg gaatgatttt acaattattg cccttgcagg
    11161 agaagtcgtg gtcgattact cactgaaaac aaaaaaatta taccctgatg aagatatttt
    11221 tgtagcaggt tattgcaccg aggtacaatg ttacatccca acagcagaaa tactgaagga
    11281 aggcggttac gaaccggtaa ccagtatgat ttattatgga ttgccagggc ctttttctga
    11341 aaatgttgaa aataaaattt ttactgcaat aaaccttgct atgaaaaaaa ctggtgcaaa
    11401 agcctacacc agaaaaaaat taaacttact aatgaataat ctgtgaactt tgtcttttct
    11461 tccttgcatc agataaaact gtaacacttt ataatactgt taaaaattgt gaaaggctta
    11521 aatctgttta ttctttattt tatactttta tcttcaaata aaaataacct taaaacacta
    11581 cactatgaaa aaaacatcag gtggcggcct taaccgccgg gaatttcttg gtctgtcggc
    11641 tcttgggtta accggcttga cgatattgcc aagctatgtt attgacggaa tgcgtatacc
    11701 tccaagcgac cggatactga tgggcacaat cggccttggc cgtcagtctc tctctgattt
    11761 caggggattt gcaggctatc caggtcttca gattgtagca tgctgcgatg ttgacacgaa
    11821 taagcaactt cgatataaga ggactattga agagtggcag aagtccaagg gacttgcccc
    11881 aagatgcgat gtctacgagc agtatgaaag gcttcttgaa agaaaagata ttgatgtggt
    11941 tgagattgtt tcccccgacc actggcatgc tcttatgacg atacatgcat gtcaggccgg
    12001 aaaggatgta tatgttcaga agcctctttc ctacacaatt aaagaggcac tggtgatgac
    12061 cagggtggca aatgatacag gccgggtggt tcaggtagga agccagcagc gttcaagccc
    12121 tgaattccag aaagctattg aacttgtaag agctggagca attgggcaca ttgaaaaaat
    12181 atatgcaaaa gtaggtgacc cgccaaaacc atttgacctg ccggaacagc caattcccgg
    12241 aacaattaac tggaatcttt ggctgggacc gcttaacgat gaaaagattg tttaccatcc
    12301 agatcttgca cctcctattt cactcgaccc acctgttaat gaatcaatat ggggtgcatg
    12361 gcgctggtac cgcgaaacag gtaatggata tacagccgac tggggggctc atatgttcga
    12421 catagcacag gcagcaatag gtatggatgg atcagggccc tatgaggtaa ttcctaaagg
    12481 ctacaacgga caaccatacc tcacattcaa atacctcacc ggggtggtaa tgactgaaca
    12541 accctatctt gaagacaatc ccactgcaca gggtattaaa tttatcggaa ctaaaggctg
    12601 gatagaagta gcacgtggat acatagcatg ctccgattca tcacttatcc cacctgaaat
    12661 tgccggaaga agacctcaac caagaacagc acaggcagga ggtcagcagg gtcaaaggca
    12721 gacacaacag gcacagggac aacagcagag gcaacaacag cagcaggcac gtcccgctcc
    12781 gacacaggaa ggactgagat tcgaaatcag ttctccgcat atgacaaact tcctcgattg
    12841 tgtcaaatca agaaagaaac ccatcgcctc aattgaagta ggatgcagta cagctataac
    12901 ctgctgcctg ggtaatatcg caaccgaact gggacgacca gtaaaatggg atcctgcaac
    12961 actgactttc gtcaacgacc ctgaagcaga aaaccacaga ctgatgcatt accagtacag
    13021 aagaccatat aaactgtgct gaaatattac cctgctttaa atagatttcc cgaaataaaa
    13081 aagataaacc tgcagaaaaa ttctctgcag gttttttctt aaaatttcgc actctgttat
    13141 cttcctccag gtatgatatt ctaaaataca actgttgtca ttccctctga tttccttagt
    13201 tcaatctccc tcgaggaacc gtttactgta actctgtatt taccatagaa agcttttgtt
    13261 gagaaagatc catcccggcc tattttaccc gtaatatttg tccaccactc cctgaaaatc
    13321 agatttctgt aagcttcggc tgcaggagtg ggagtccagt cgcgcctgta cagtgaagaa
    13381 accggtatcc agtttgcccc ttcccagaaa ccccacataa gaatcccctc aacggcagga
    13441 tatgcaaaac aaatacggta ataatccaca agctcttttg ctttaagttc ttcttcttca
    13501 ggtgtcaaag tatttatttt ttcccggtaa tattttgaac gctgacctgg aatgttgaat
    13561 tcggtaattc ttatgggcaa accaaaaatt gccagcgaat caagagctct ttgcagctga
    13621 ttcctgtcaa atgtttcggc atgcagatgc ccctgaactc ctattcctga aataggaacc
    13681 ccttgcctca gcagtaatct aatattagac atgtaatcaa ccagcttaac accggtaaga
    13741 atgtcataat cattcagcca gagctttgca tcagggtcac cattgtgaac ccattgagcc
    13801 attagtttcg tgatttcagg gccaagacgt tcctcgtaat aattaccatg tatcatttca
    13861 ttattcaaat catattcagc aaaccgtcct ttatactttg ctgcaattgt ttcagccctg
    13921 ttttttatca cttgcttcag ttcatcatct ttcagttcct taacccatgg ctgtatgaac
    13981 tggggaatgc cccagaaaag gttatgaccc cgtaaaggaa ttccgttagc ttcgcaccat
    14041 cccagtatca tatcaactac cgtgtaattc acatcccctc tgcgtggttc catatttgcc
    14101 cattttaaag cattttctgt tactgcagca ttaaaattct caagaaactt ctccttgtat
    14161 attcttttgg tttcctcact ccacgaatta cctgccagac cgttgctgat agcacaacca
    14221 aaccagaact catgtctcaa ttgttcaatt ttaaagctac tacctggctt tgctttaaca
    14281 acaattatac cttgccggta tttcgctatt gaatcattaa ttccggggaa ctgtgctttg
    14341 cattccccgc ctgaaaccaa taacgccaga ataaaaacgg caatcagaat ttcttttaat
    14401 gcagatgtaa tatttattct cattttttaa aaaaataaat taacagtatc tgtttttctt
    14461 tttgatcttc taaacattta agaattcaag ctctcaaata gttttcacat cctgcaatca
    14521 aatagatcta tttttatgca agatataact atttatagac atacctgtaa aatgttcttg
    14581 aaacggtcat ttatattaaa acgtcctgcg ggctatttta atttttgtta ttaaattatt
    14641 gattcagttt aattattatt ataactttat cattgcttcc agtagcgttc tttatggcaa
    14701 aaacggaagt ttatgaaaaa atccactttc atatcagttt tctgttttgt attcattacc
    14761 agcactaata tttcttttcc ccagtttgac agaagagctg aagagctgat ggttctttta
    14821 aaatcaggca aacaaagaaa ccttctctct atagctagac gtgtagactc agtaaattat
    14881 agagatgaaa cggatagaac gctgttaatg tatgcttcgg gattaggtaa tactgaactt
    14941 tgcaaaattc ttgttgaaaa aggcgctgat gtgaatttaa caacacgtga tggtgttact
    15001 gcacttatgt ttgcagcatc ttatggtcat gcacttactg caaagtttct tgttgaaaac
    15061 ggagcgcgat tagatataag atccgaaaat ggcttcactg ctcttgcgat tgcttcaaaa
    15121 aacggacata cagaagtagt aaaaacactg ctcgacaatg gagattctcc tgatactaga
    15181 ataacagaag gatacacttc tttaatgatt gctgcagaaa acggatttca ggaaattaca
    15241 aaattacttc ttgaaaaagg tgcaaatcct gacctgcgta caacagacgg atacactgca
    15301 ttgatccttg cttcccagaa cggaaatatt ggcgttgtaa aaacccttat tgattataag
    15361 gccaacctcg atttacagac agacgatggt attactgccc tgattatggc aacacggtat
    15421 ggttattctg acattgttaa acttctggct tatgcaggag caaatccgag tctccagtgc
    15481 gctgatggag aaaccgcact atttatagca gcaatgaatg ggtataccga aatagttaag
    15541 accctgctgg agaaaggggc agatgtgtgt ctgccttgca acgatagcgc aacacctctt
    15601 tttattgcat ctcttaaagg ttatactgaa atagcctcaa tgataattga aaatgaaaaa
    15661 gccagaggag tttcctgcgt ggatgaaatt aagacattaa tgatgactgc caatgatgaa
    15721 atggcagaga aggttaaatc aatactggaa aaaaggtcaa aaagcaaaac tgaattcgca
    15781 aagggaattg gtgcaaaagt ttatacaaca tcaaacggcc atatagaagt agttgaattc
    15841 atgctcgata aagagcctga tcctgaaata gaaaaaagcg gaaaaagacg aacatcactc
    15901 atgcgggcta gccagagcgg aactgttgaa ctggtaaaaa tgctcataaa ggataatatc
    15961 aaaaaagatt atccttttga tgaaaaatat tctgccctga tggttgcctg catgaacggt
    16021 catgcagaaa taataaaaat gctgcttgat aaaggttctg atgtaaatta cagattatcc
    16081 gacggaacaa cagcacttat atacgcagtc cagaacggta attcagaaat agtcaatatg
    16141 cttcttcaaa gtggagcaga tattaacaac atttgcaggg atggctctac agcacttatt
    16201 catgcttcat caaaagggaa aaccgaagtt gttaaactcc tcctagaaag aggtgcccgt
    16261 catgatctga aacgaaacga tggttcaaca gccctgacaa ttgcctcagg tgagggtttc
    16321 agagatatag tatcactgtt actggataaa ggtgccgacc cagaagttag agaaaggaac
    16381 ggatatacac cttttatgct cgctgttaaa aacggatata ctgacattgt aaagctcttc
    16441 ctggacagaa tatccaatcc tgattctcat acaaatgacg gagttacacc tttaatgcag
    16501 gcttgcatag gaggatacac tgaaattgcc cgaatgcttc ttggaaaggg ttctgagatt
    16561 aatgcccgca cacccgacgg atacactccc cttatgctgg cagcacttaa cggtcatatt
    16621 gaaacagtca aatttctgat cgacagaggt gcagaaataa accagcagat gacaaaagga
    16681 actactgccc taatgcttgc tgcaagcaat atgcacctcg atgttgtcag actgctcctt
    16741 gataaaggtg caaaacccga cattaaagac aatgacggta aaacagcaat cgactatacc
    16801 gaatctttcg caattaaaaa tcttataaag aaatacacga aataagaaag ggaaagtgtt
    16861 tcaaccttcc cttttctcct tgtaggagcc tgtttaaatt tttgtgtaaa ctggcagcag
    16921 ccgggattaa aatatactgc atctatcacc aaaaataatc attaattggt taataactgt
    16981 gccccaatct ctga
//
LOCUS       DAOSNB010000019        16658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3490_length_16658_cov_4.180148, whole genome shotgun sequence.
ACCESSION   DAOSNB010000019 DAOSNB010000000
VERSION     DAOSNB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3490_length_16658_cov_4.180148"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2364
                     /locus_tag="PLG42_01755"
     CDS             <1..2364
                     /locus_tag="PLG42_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU29967.1"
                     /translation="QLIETAWQLASRNDRETLEILDNVIVLLVHANPDGQELVSSWYM
                     QESDPQKRRMNIPRLYQKYIGHDNNRDFYMLAMRETRNMARQMFIEWIPQILYNHHQS
                     GPAGTVLAGPPYRDPFNYLFDPLLITSIDALGAAMNNRLNAEGKPGATQRTGASFSTW
                     YNGGLRTTAYFHNIAGLLTEITGSPTPSEIPLVIDRLLPNGATPNPVTPRKWHFRESI
                     DYSISLNYAVLSYASRHRSEVLYNIYRMGMNSIERGSRDNVTITPRIIEAMKKLAESD
                     TAARSSSASGNFAGQQQPRIPLKYYEQVMNDPAFKDARAYIIPSDQTDFPTAVRFVNA
                     LILTGIEVHKASGSFTVNGKTYPAGSYVIKTAQAFRPHVIDMFEPQDHPNDLQYPGGP
                     PIPPYDAAGWTLALQMGIKFDRVTDDINGPFIKLPVGVTEPFPAQTLPAGAKAGYLLT
                     PAINNSFRIVNDLLAAGVEVFRVTKDEKNKSNVRAGDFFVPVAARRLLAELISEYGVK
                     VKPASNRPAAMERIVPARIAVWDRYGGSIQSGWIKWILEQYNFPYRTVFSQGIDSGSL
                     KQKFDVIIFPPGAIPAYRGAVQEGQGTFQRQQPGEDVPEPYRNWTGSITAESSIPQLK
                     KFIEEGGIVVATGTSTNLAYHLQLPVRNALVKKSDDGRETPLSNSEFFIPGSLLSVAV
                     DSSLAEAWGMQSRGIIFFDRSPVFSLGDDAETRGVRRLLWFTDEKPLASGWALGQQYL
                     KGNCAGFTAAVGKGKLVAYGPDITFRSQSQGNFRLLFNQLYSYQIKR"
     assembly_gap    2529..2628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2631..3872
                     /locus_tag="PLG42_01760"
                     /pseudo
     CDS             2631..3872
                     /locus_tag="PLG42_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(3955..4659)
                     /locus_tag="PLG42_01765"
     CDS             complement(3955..4659)
                     /locus_tag="PLG42_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOU29968.1"
                     /translation="MKKIFDYFDHRYRESIMSRSASQDGPVITISRQTGCDARQIAEM
                     VVDNLNRKYYTNKWKWIDKDIIYKVAEELNTDPNRIENFYKGIELTDISEMIMAFSGN
                     FVSDLRVKKAIKDVVLSMCKEGYIVFVGRGSVAIARDFQNALHIRLIAPFYWRVENVM
                     KKKEMDIETAEEYVVDTDEKRYNLILNFLDKKPLSIDYLFDAIINRSSFTIQEIADFI
                     TAMYEKKIAKRLAATV"
     gene            4950..5492
                     /locus_tag="PLG42_01770"
     CDS             4950..5492
                     /locus_tag="PLG42_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU29969.1"
                     /translation="MKNLLKYSTLIISILLFTACKKIHVVSEGQKIFFQYEYVNYSSG
                     FQHYGFIIDNEGNVLTFNKPEKWNFPGSNKILTQAEIEENISSCTPSGQKIPENELKK
                     FVNYIDNIAASKVSAPRTRHRASKAGTLSYYCFIFSESDSEYKVVTVKTEGEVECENL
                     NFFSRRVIEWMRDINRSLNK"
     gene            5623..6078
                     /locus_tag="PLG42_01775"
     CDS             5623..6078
                     /locus_tag="PLG42_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOU29970.1"
                     /translation="MINFILSTPELLWFLLGLILFLLELILPGFVIFFFGVGAWTTAF
                     VCLLFNPGVDIQILIFALTSVLSLVALRRMIQKKFFYGKNDNSHLIEDEFTGKEAITL
                     SEIKPGKKGKVEFKGTVWTAESDTDIDEGETVIIISKDNFNLKVKPKNK"
     gene            6081..7025
                     /locus_tag="PLG42_01780"
     CDS             6081..7025
                     /locus_tag="PLG42_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HOU29971.1"
                     /translation="MLASTTTFILLGLILLGFILISSAVKIVPQRTAIIVERLGKYKA
                     TYTAGFQIIIPFIDKVRYRHTLKEQAMDVAPQVCITKDNIAVEVDGILYLQVLDPMKA
                     SYGIDNYKFASIQIAQTTMRSVIGKLELDKTFEEREAINNAIVEAVDKASEPWGVKVT
                     RYEVKNISPPQSIRDAMEKQMRAEREKRAIIAESEGTKQAKINVAEGEKQELIKKSEG
                     EMQRRINEAAGRASEIEQIAKATANGLRAIAQAISEENGLNAVNLRIAEQYLTEFGKL
                     AKQNNTIILPSNLTDVGGIIATATAVFNEVKDKQKKQS"
     gene            complement(7106..9469)
                     /locus_tag="PLG42_01785"
     CDS             complement(7106..9469)
                     /locus_tag="PLG42_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOU29972.1"
                     /translation="MTERVRKLREQSLNAEETISAERAILITQFYKSDEARGLSAPVL
                     RARAFEYLLRNKHICINEGELIVGERGPAPKATPTYPEICVHSIKDLEILNSRPKVSF
                     KVSKEVFEVYEKEIIPFWKGRSNRDRIMSLMTPEWLDAYEAGVFTEFQEQRAPGHTVL
                     GYKMFKTGFLQLKEEIKASIAKIDFYNDPDALRKLEELKAMDIACDAIIMYARRHAEE
                     LEKMAQKEPNQGRKKELEKMAAICRKVPAHAPETVHEMLQHYWFIHLGVITELNPWDS
                     FNPGRLDQHLLPVFKNDIESGNASRDELYELLCCFWVKFNNHPAPPKVGVTAQESNTY
                     TDFCLINLGGVKPDGSDGVNEVTYMLLDVIKEMRLVQPSSMIQVSKKSPDRFVHKALD
                     IIKTGFGQPSVFNTDAIIQELLRQGKDITDARNGGASGCVETGAFGTECYWLTGYFNM
                     PKVFEITINNGIDPRTGKQIGLKTGEVESFKTFEELVEAFRKQVNYFIDIKIRGNNVI
                     SKTFAEFLPVPFLSLLIEDCIANGKDYNNGGARYNTTYIQGVGLGSMADILTSVRYNI
                     YDKGKYSWEEVKEALQADFKGFEQMQYDFLYETPRYGNDDDYADRHAVKVFEIFYDAV
                     NGRPDLRGGVHRINLLPTTSHIYFGSVTGATPDGRNARQPLSEGISPVQGADHEGPTA
                     VIKSASKIDHLKTGGTLLNQRFSPSFFNDENSYANLTALIRSYFSLDGHHIQFNVVST
                     ETLRDAQKHPEKYRDLIVRVAGYSDYFNDLGEDLQNEIIRRTEHEEI"
     gene            complement(9504..10304)
                     /locus_tag="PLG42_01790"
     CDS             complement(9504..10304)
                     /locus_tag="PLG42_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOU29973.1"
                     /translation="MKGLVFSIKRYSIHDGPGIRVTVFMKGCPLSCWWCHNPEGISPE
                     PEEVNEIKRIGKKEFSVKKMAGEYYEPEDIVALIERDRVFIERSGGGITFSGGEPFMQ
                     PEFLLETLKVCRKAGFHTAIDTSGHFPSELLPEVLPYTCLFLFDLKHLDPEKHQKFTG
                     VTNTLILKNLQLMLKSDVEIMLRIPVIPGINDDEQHLFELRKYISEIKTGRIRMINLL
                     PYHKIGASKYRRLNREDKMKDYNTPSSERMKELKEFFSATGIKVKIGG"
     gene            complement(10717..12027)
                     /locus_tag="PLG42_01795"
     CDS             complement(10717..12027)
                     /locus_tag="PLG42_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU29974.1"
                     /translation="MKKNLLLVPFLLFLLESAGQQPEIILKKGWNFGFLPAVAYDSDL
                     GIYYGIIINPFDYGDGSVYPDYLQSVFLQVSGYSGGSSEHILEYVSYSLLPSTRFSTG
                     IKYTGYKAYPFYGYNGNESYYNVDWENDESSAYRTRMFYRLARKNFRILTDIQDTVGN
                     TNYQWHLGFMANYCRTGAVDIVRMNKRLPASRQLPDTATLYDKYCEWGLLDDAERDGG
                     ITGTLMAGLIYDSRDRLTCPGKGVFSEVNLLWMPPFLCEKNYSYLKFGLIHRQYITLV
                     TRRLIFAYRFWLNVNLAGDQPFYTRQLIAGFQGNEGYGGYSTLRGVLMQRIVAEGFIL
                     GTAELRSRLVNFRFINQNWYIGAVAFIDAGRIIKPVSLNLSNVPQADYEIFFRPSDRS
                     LHKSAGGGLKLAMNENFVLSAEFAAPFDRQDGVSGLYLGLGYQF"
     gene            12158..14266
                     /locus_tag="PLG42_01800"
                     /pseudo
     CDS             12158..14266
                     /locus_tag="PLG42_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857936.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
     gene            14328..16358
                     /locus_tag="PLG42_01805"
     CDS             14328..16358
                     /locus_tag="PLG42_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOU29975.1"
                     /translation="MPVNVSIAGKEPVAVKGVLDLREYKPGDRIKVNLNGEWEFYWNR
                     FLYPSDFTTSEKRPQPDIYGKVPAYWTSYSGEIPVNKYGYAAYRLVILLPENTETSYA
                     FDIPVFDSSFELWVNDSLLYSNGKPGTTSETTIPEYRPGLRVYNSSSDTLSILINVAN
                     FHHRRGGFWQTMVFGTYKDVQKSVASKLAADYITVGILGSFALFFMLFFLLYPRDKIL
                     LIYSLTLLFLAIRPFFTSEYLIYDYTDVSWSWTIRYEYLCSFLALGGWAWYNYYLYPS
                     KYNKAFSQSLTVLFVASIIAILATSVKVFSYSVFILYIGIVSLVAHALISSFKGILKK
                     NITDAFYFILFLIISFGALHDIFVSAGMTKSSFGYLMPFLMVIFLFAQSVLLLSKWIK
                     AFYEKERLQVELEKINRNLENIVAERTRELTLRTKEIEKKNLMIEAQNLRLTETINFK
                     NKIFSVIAHDLRSPVVNILYILNLLKEKEFKDKYEEFADSSIKYAQMVIALLENMLVW
                     GRGQEDKIKYSPGMHDLAVMIQTNISIFKETANRKKISISFNQEGNSMAYCDKDLIDI
                     VIRNLLSNAIKYSYPGGIVSVLVKENKNENTLSVKVCDNGTGIPEEHLKNLFNPTEII
                     STPGTENEKGTGIGLKLCHELMLINKGHISAESHEGSGSCFQIVLPSSPVLL"
     gene            16427..16573
                     /locus_tag="PLG42_01810"
     CDS             16427..16573
                     /locus_tag="PLG42_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU29976.1"
                     /translation="MLRDIIRIDEELCTGCGLCVPNCHEGALQIIDGKVRLISELMCD
                     GLGA"
BASE COUNT         5088 a         3510 c         3329 g         4631 t
ORIGIN      
        1 cagcttattg aaacagcatg gcagcttgcc agccgcaacg acagggaaac gctcgaaata
       61 ctcgataatg tcattgtgct gcttgttcac gccaaccccg acgggcagga gctggtttca
      121 tcgtggtata tgcaggagag cgatcctcag aaacgaagga tgaacatacc aagactttat
      181 cagaaatata tcggtcacga taataacagg gacttttaca tgcttgccat gcgggagaca
      241 cgcaatatgg cacgccagat gttcatagaa tggattccgc agatcctcta caatcatcac
      301 cagagtggtc ctgcaggaac tgttctcgca ggtcctccct accgtgaccc gtttaattat
      361 cttttcgatc cactgcttat cacaagcata gatgctctcg gagctgccat gaataaccgc
      421 ctcaatgctg aaggtaaacc aggtgctaca cagcgcacag gtgcttcatt ttctacctgg
      481 tataatggtg gactgcgtac aacagcatat ttccacaata tagcagggct gctcacggag
      541 ataaccggaa gtccaacacc gtcggagata cctcttgtca ttgacaggct tcttcccaac
      601 ggcgccactc caaatcccgt tacaccccgt aaatggcatt tcagagagtc aatagactat
      661 tccatctcac ttaactatgc agtactcagc tatgcaagcc gtcaccgcag cgaggtgctt
      721 tacaacattt accgcatggg aatgaattca atcgagcgcg gcagcagaga caacgttaca
      781 ataacccccc gcataatcga agctatgaag aaactggctg aaagtgacac tgctgcaagg
      841 agttcttctg cttcaggaaa ttttgcgggt caacaacagc ccaggatccc attgaagtat
      901 tacgaacagg ttatgaacga tcctgctttc aaggatgcac gggcatatat aattccttct
      961 gaccagactg actttccaac agcggtaagg tttgtcaacg ctcttatcct cacaggcata
     1021 gaagtgcata aagcttccgg aagttttacc gttaatggaa aaacctaccc ggcaggttct
     1081 tatgtaataa aaactgcaca ggctttccgc cctcatgtga ttgacatgtt cgaacctcag
     1141 gatcatccta acgatctcca gtacccgggt ggtccaccca taccaccata tgatgcagcc
     1201 ggatggaccc tggcattgca gatggggata aaattcgacc gtgtcacaga cgacattaac
     1261 ggacctttca tcaaattgcc ggttggcgtt actgaaccat tcccggcgca aacactgcca
     1321 gcaggagcaa aagctggata tctgctcaca cctgcaatta acaattcatt cagaattgta
     1381 aatgatctgc ttgctgcagg tgttgaagtt ttcagagtga caaaggatga gaaaaacaaa
     1441 agtaatgttc gtgcgggtga tttctttgtt ccagttgcag caaggagact ccttgcagaa
     1501 cttatctcag aatacggagt taaagtgaaa ccggcatcaa accggcctgc tgcaatggaa
     1561 agaattgttc cagcaaggat tgctgtctgg gatagatatg gaggttccat acaatcggga
     1621 tggataaaat ggattcttga gcagtataac ttcccttacc gtacagtttt ctcacagggc
     1681 attgattcag gaagtctgaa gcagaaattt gatgtgataa tcttcccccc tggtgccata
     1741 ccagcctaca gaggagctgt acaggaaggt cagggcactt ttcagcggca gcaaccaggc
     1801 gaagatgtgc cggagcccta taggaactgg acaggctcaa taactgccga aagttctatt
     1861 ccacaactta aaaagttcat tgaggaagga gggatagtcg ttgctaccgg aacaagtaca
     1921 aatcttgcat accatctgca gttgcctgtg agaaatgcac tcgtaaaaaa atcggatgat
     1981 ggccgtgaaa caccgctaag caacagtgaa ttcttcatac ctggaagctt gctgtcggtg
     2041 gcagttgatt catcgcttgc tgaagcatgg ggtatgcaat cgcggggaat aatatttttc
     2101 gacagaagcc ctgtattcag tctcggcgac gatgcagaaa caagaggtgt cagaagactt
     2161 ttatggttta ctgatgaaaa gcctcttgcc agtggctggg ccctcggaca acaatatctt
     2221 aaaggaaact gtgcaggttt tactgctgct gttggaaaag gaaaacttgt tgcatatgga
     2281 cccgacataa cattccgcag ccagtctcag ggcaacttca ggctactttt caaccagctg
     2341 tacagctatc agattaaaag atgactgaca gcattgaaat tcagataaaa taatgtcata
     2401 agtcatatgt ttttttcagt tttagttata aattgtaaca attttaaata actaaacaat
     2461 ttaattaata tacatatgcc taaagtcaca aaggaagatg ctcttctgta tcatagcagg
     2521 ggacggaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat atgcctaaag
     2641 tcacaaagga agatgctctt ctgtatcata gcaggggacg gaagggaaaa cttgaggtaa
     2701 ttcccacaaa accatacgga acccagttcg acctcagtct ggcttacacc cccggtgttg
     2761 cttttccatg cctcgagatt gagaagaacc ccgatgatgt ttaccagtat actgccaaag
     2821 gcaatcttgt tgcagtgata tcgaatggca cggcagttct tgggcttggc gacataggag
     2881 caatggcagg taaacctgta atggaaggca aagggctgct tttcaaagtg tttgccgatg
     2941 ttgacgtctt cgatattgaa attgacgaaa aagatccgga aaagctgatt gagatatgtg
     3001 caaaaattgc tcccacattt ggaggaataa acctggagga tattaaagct cctgaatgtt
     3061 ttgaaattga aaagcgactg atagagatgc tcgacatccc tgtcttccat gatgaccagc
     3121 atggcacagc aataatttca ggagcaggat tactgaatgc tctcgagatt acagggaaaa
     3181 atatagaaga cctgaaagtg gttgtaagcg gagcaggtgc cggggctatt gcctgtacaa
     3241 aattttatgt atcgcttggc gtcagaaaag aaaacatcgt aatgaccgat agtaaagggg
     3301 tattgaacaa aaagcgaaag gacttgaaca aatacaagtt ggaatttgtg actgacagaa
     3361 atattgacac tcttgctgaa gccatgaaag gggctgatat gtttctggga ctttcggttg
     3421 ccaatgtcgt tacaagggag atgcttgcct cgatggcgcc caatccgata gtgttcgcaa
     3481 tggcaaatcc tgtccctgaa atttcttatg aagatgcaat ggcaacccgt aacgacctta
     3541 tctttgcaac aggaaggtcg gattacccaa atcagataaa taacgtgctc ggattcccat
     3601 atatcttcag gggtgcactt gatgtaagag cctcagtcat taatgaagaa atgaaacttg
     3661 cagcttcaca agcactggca agccttgcca aagaacctgt gatacctgaa gtattgaaag
     3721 catataacct gaaagaacta aaattcggca aggattatat aatccccaaa ccactggatc
     3781 ccagactgat atcaactgta tctcctgctg ttgcccgagc agcaatggat accggagtag
     3841 caaagttccc gataaccgat tgggaagcat ataaaaagag cctcgacagg aggctgaggg
     3901 atcatatcca gaaggttgca ggacttattg gcatttagcc tgaagaaaag cgatctaaac
     3961 agttgctgct aaacgctttg caatcttctt ctcatacatg gcagtaataa aatctgctat
     4021 ttcctgaatt gtaaaagagc ttcgattgat aattgcatcg aaaagataat caattgacaa
     4081 tggcttttta tcaaggaaat tcagaatgag attatacctt ttctcgtcag tgtcaacaac
     4141 atattcttcg gctgtctcaa tatccatctc tttctttttc attacgttct ccaccctcca
     4201 gtagaatgga gcaataagcc ttatatgaag ggcattctga aaatcgcgtg caattgccac
     4261 actacctcta cctacaaaga caatataacc ttccttgcac atggaaagaa ccacatcttt
     4321 aattgccttt tttaccctta aatcactgac aaaatttccg gaaaaagcca ttatcatttc
     4381 agaaatatct gtaagctcaa tacccttgta aaaattttct atacggttag gatccgtatt
     4441 aagctcttct gcaaccttgt agattatgtc cttgtcaatc catttccatt tgttagtgta
     4501 gtacttcctg ttcaggttat caactaccat ctcggcaatc tgccgtgcat cacatcctgt
     4561 ctgcctcgat attgttatta cagggccatc ctgcgaggca gacctgctca ttatcgattc
     4621 cctgtaacgg tggtcgaagt aatcgaatat ttttttcata accttaaatt ttatatccct
     4681 tcagtgggaa atatttaaaa agtttattat caaccactta tcccgtattt gaaaacggca
     4741 gaaatacagg gtaattataa aacaacccgc tagaatcttt tgccataatt tacgaaaaaa
     4801 aattgtaatc aggaaattta ttcagatact tttctgaaaa aatatctctc cataggaatg
     4861 cgttataatt attgaaaaat ttaaataacc tgcttctact ataaaaaatt gagtatggcg
     4921 cgatttttgt atatttgcat caatcagaaa tgaaaaactt actaaaatat tcgacgctta
     4981 ttatcagcat attactcttc accgcatgta aaaaaataca tgtcgtgagt gaaggtcaga
     5041 aaatcttctt tcagtatgaa tatgtgaatt attcttctgg ctttcagcat tacggattca
     5101 ttatcgacaa tgaaggcaac gtcctgactt tcaataaacc cgagaaatgg aacttccccg
     5161 gttcgaataa aatacttacc caggcagaaa tagaagagaa tatctcatca tgcactcctt
     5221 caggccaaaa gatacccgaa aatgaactta aaaaatttgt aaactatatc gataatatcg
     5281 cagcaagtaa ggtttctgca ccgcgaacca gacacagggc ttcaaaagca ggaacactga
     5341 gctattactg ttttattttc tcggagagcg actcagaata taaagtggta acggttaaaa
     5401 ccgaaggtga agttgaatgc gaaaatctca acttcttttc aagaagagta atagaatgga
     5461 tgagagacat taacagatct cttaataaat aaatctgtta ccttaaatta atttcctgta
     5521 attcacaaaa aaatttttaa taacctgtaa tccctgctca ttttgttata tctttactca
     5581 ttatgaattg ctgtaattta tttagataaa acaaccttta atatgataaa ttttattctt
     5641 tcaacacccg aattattatg gtttttactg ggacttatcc tgtttctact ggaactcata
     5701 cttcccggtt ttgtaatttt cttttttggg gttggagcat ggacaacagc atttgtatgt
     5761 cttcttttta accccggtgt tgatattcag attctgattt ttgcattaac atctgttcta
     5821 tcactggtcg cgcttcggcg tatgatccag aagaaattct tttatggaaa aaacgacaac
     5881 agccatctga tagaagatga atttaccggg aaagaagcca taactctctc tgaaattaaa
     5941 ccgggcaaaa aaggcaaggt agaatttaaa ggtacggtct ggacggcaga atctgatacc
     6001 gatattgatg aaggtgaaac agtaataatc atcagtaaag ataatttcaa ccttaaagtc
     6061 aaaccaaaaa ataaataaca atgttagctt caaccacaac attcattctt ctcggattaa
     6121 tccttctcgg attcattctg atcagtagtg cggtaaaaat tgtaccacag agaactgcta
     6181 taattgtaga gcgtctcggc aaatacaaag caacttatac agcaggcttt cagattatca
     6241 ttcctttcat cgacaaagta aggtacaggc acacccttaa agaacaggcg atggatgttg
     6301 caccccaggt atgtatcaca aaggataaca ttgctgttga ggttgatggt attctttatc
     6361 ttcaggttct tgacccgatg aaggcatcgt acggaataga caattacaaa tttgcttcaa
     6421 tacagatagc ccagacaacc atgagaagtg tcataggcaa gctggagcta gacaaaactt
     6481 tcgaggaaag ggaagcaata aacaatgcca ttgttgaggc ggtggataaa gcgagtgaac
     6541 cgtggggtgt aaaggtaaca cgttatgaag taaagaatat ttctccgccg caaagcataa
     6601 gggacgccat ggagaagcag atgagggctg aaagggaaaa gagagctatc atagccgaat
     6661 cagaaggtac gaagcaggca aaaataaatg ttgctgaagg cgagaagcag gaacttatca
     6721 aaaaatcgga aggtgaaatg caaaggcgta tcaatgaagc cgccggacga gcctcagaga
     6781 tagaacagat agcaaaggca acagcaaacg gactgagggc aattgctcag gcaatatcag
     6841 aagagaatgg acttaatgca gtgaacctca gaatagctga acaatacctt accgaatttg
     6901 gaaagcttgc aaaacagaat aatacgatta tcctgccttc aaaccttact gatgtaggag
     6961 gaatcatagc cactgcaaca gcagtgttca acgaagtgaa ggataagcag aaaaaacagt
     7021 cctgatattt tcaggaaact gatttaagac tttaagacag aaccggattt agtgtaccgg
     7081 agcggggaaa gctattatgc aaccgctaaa tttcttcgtg ctctgtccgc cgtattattt
     7141 cattctgcag gtcttcaccc agatcgttga aatagtcgct gtatcctgca acgcgcacaa
     7201 tcaggtcacg atatttttcc ggatgtttct gtgcatctct cagggtttct gttgaaacca
     7261 cgttgaactg aatatgatgt ccatcgaggc tgaaataact tcttataagt gctgtaaggt
     7321 ttgcatagct gttttcatcg ttaaagaagg aaggtgagaa tctctggttc agcaatgtcc
     7381 cgcctgtctt aaggtgatca atcttcgacg ctgacttaat aactgccgtg ggaccttcat
     7441 ggtcagctcc ctggactgga gaaattccct ccgacagggg ctgcctggca ttacgcccgt
     7501 cgggtgtagc tcctgtcaca cttccgaaat aaatatgtga agtggtcgga agtaaattta
     7561 ttctgtgaac tcctccccgc aaatcgggtc tcccattcac tgcatcataa aatatctcga
     7621 aaacttttac ggcatgcctg tcggcataat catcatcatt accgtatctg ggtgtttcgt
     7681 acagaaaatc gtactgcatc tgctcaaacc ccttaaagtc agcctgcaac gcttccttga
     7741 cctcttccca tgaatatttg cctttatcgt aaatattgta cctgaccgaa gtaagtatgt
     7801 cagccatact tcccagccca actccctgaa tataagtagt attgtatctg gctcctccgt
     7861 tgttgtaatc tttgccatta gctatgcagt cttctataag aagagacagg aatggtacag
     7921 gaaggaactc agcaaatgtc ttgctgatca cattattccc tcgtatcttg atatcaataa
     7981 aatagttcac ctgtttcctg aaggcttcaa caagttcttc aaaggtcttg aatgactcta
     8041 cctctccggt tttaagacct atctgctttc cagtgcgtgg gtctataccg ttgttaattg
     8101 taatttcaaa aacctttggc atgttaaagt aacctgtaag ccaataacat tctgtcccga
     8161 aggctcctgt ctccacgcaa cctgaggcac ctccgttacg ggcatcggta atatccttcc
     8221 cctgtcgcag aagctcctgg ataatggcat cggtattgaa caccgaaggc tgcccgaacc
     8281 ctgtttttat gatgtcgagg gctttgtgaa caaacctgtc gggcgacttt ttgcttacct
     8341 gaatcattga gcttggctgc accagcctca tctcttttat cacatcaagc agcatatatg
     8401 ttacttcatt cactccatcg ctgccatcgg gctttacacc tccaaggttg atcaggcaga
     8461 agtcggtata agtattgctt tcctgtgccg ttactcctac tttgggaggg gcaggatgat
     8521 tgttaaactt tacccagaaa cagcatagca gttcataaag ctcgtctctg gaagcattcc
     8581 ccgattcaat atcatttttg aatacaggca gcaggtgctg gtcaaggcgt ccgggattga
     8641 atgagtccca cggatttagt tcggtgataa ctccaaggtg gatgaaccag tagtgctgaa
     8701 gcatttcatg taccgtttct ggagcatggg caggcacttt gcggcatatt gccgccattt
     8761 tttcaagttc tttctttctt ccttgattcg gttccttttg agccatcttt tcaagttctt
     8821 cagcatgacg gcgggcatac ataattatcg catcacatgc aatatccatt gctttaagtt
     8881 cctccagctt tctcagtgca tccgggtcat tgtaaaaatc aatcttagct atacttgcct
     8941 ttatctcttc ttttaactgc agaaagccag ttttgaacat cttatatccc agtaccgtat
     9001 gtccgggagc ccgctgctcc tgaaactctg taaacactcc tgcttcatat gcatcgagcc
     9061 actcaggggt catcaaactc ataatcctgt cgcggttact cctgcctttc cagaagggga
     9121 taatctcttt ttcgtaaact tcaaaaacct ctttgcttac cttgaatgat actttaggcc
     9181 tgctgttcag tatctcaagg tctttaatgc tatgaacaca tatctcagga taggtgggtg
     9241 tagctttagg ggcaggacct ctttcaccca caatcaattc accttcattt atacatatat
     9301 gcttgttgcg aagcaggtat tcaaatgctc tggctcttaa taccggagcc gacaaacccc
     9361 tggcctcatc acttttgtaa aattgcgtta tcagtattgc cctttcggct gaaatagtct
     9421 cctcagcatt aagactctgt tcccttagtt ttcttactcg ttcagtcatg gcttctaaaa
     9481 ttttttcaga tttaacaatt atatcaccct ccaatcttaa cctttatacc tgtggcggaa
     9541 aaaaattcct tcaactcctt catcctttcg gatgatggtg tattataatc cttcattttg
     9601 tcctcacggt ttaatctcct gtactttgag gcacctattt tatggtaagg taaaagatta
     9661 atcatcctta ttctgcctgt ttttatttca ctaatgtatt ttcttaactc aaaaagatgc
     9721 tgttcatcat cattaattcc tggaataaca ggtatcctca gcattatttc gacatcggat
     9781 ttcagcatta gctgaagatt cttcagaata agtgtatttg taacacctgt aaatttctgg
     9841 tgtttttccg gatccaggtg tttcaggtcg aacagaaaaa ggcacgtata tggtaaaact
     9901 tccggaagaa gttccgacgg aaaatgccct gatgtgtcaa tagcagtatg gaaccccgct
     9961 tttctgcaca ctttcagtgt ttcaagcaaa aattcaggct gcataaatgg ttctcctcct
    10021 gaaaaagtaa tgccccctcc agatcgttct atgaaaactc tgtctctttc aatcaatgca
    10081 acgatatcct ccggttcata atattcacct gccatctttt ttacagaaaa ctccttcttc
    10141 cctattcgct ttatctcatt aacttcttca ggttcaggtg atataccttc agggttatgg
    10201 caccaccagc acgaaagcgg acaacctttc atgaaaacag tcaccctgat gccaggacca
    10261 tcatgtattg aataacgttt tatactgaaa acaagacctt tcatgtgcta acagaaaaat
    10321 tatgcgtgga gatattaagc ctgaaaaaag tgattaacag ccgtagaaca taaggcagca
    10381 atagagtctg cggccaagac ggcacaacat aaaactgtat ctgagttttt ctagcacagt
    10441 ttgcatgata aatgaggatg caagttaaaa actattttaa tatgttgtat tttaaattca
    10501 tattgctggt tgattttaac gcaatcttat agtatgtaac ttttatgaaa attttaacat
    10561 tttttatgca acactggctc gttttttgcg tatttatgtt ggttgacatt aagttacatc
    10621 tctaccagcc aaccggcaga ttcaatcagg ttttagtttg acaggagggt agaaaaggcg
    10681 ccgcccggcg ccttcctctt ttttatggta taatgtttaa aactgatatc caagtccaag
    10741 ataaaggccc gaaactccgt cctgcctgtc gaaaggtgct gcaaattcag cagaaagaac
    10801 aaagttttca ttcattgcaa gcttaagtcc gcctccggcc gatttatgca gagacctgtc
    10861 ggatggtctg aaaaaaatct cataatcggc ctgcggcaca tttgacaggt taagacttac
    10921 gggttttata atacgtccgg catcaataaa agctactgcc ccgatatacc agttctgatt
    10981 gataaacctg aaatttacca ggcgcgacct taattctgca gtaccaagga taaatccttc
    11041 tgccactatt ctctgcatca gcactcctct cagtgtagaa tagccgccat atccttcgtt
    11101 cccctgaaaa cctgctatca gctgacgcgt ataaaaaggt tgatcccctg caagattgac
    11161 attcagccag aacctgtatg caaaaatcaa acggcgagtg acaagagtaa tgtactgacg
    11221 atgtattaac ccgaacttga gatatgaata gtttttctcg cacagaaatg gaggcatcca
    11281 caaaaggtta acctctgaaa atactccctt tcccggacat gtaagccggt ctctcgaatc
    11341 atatattaaa cctgccataa gggttccggt tatacccccg tctctttcag catcgtcaag
    11401 aagtccccac tcacagtact tgtcgtacag agtggctgta tcaggtaatt gcctgctggc
    11461 aggaagtctt ttattcattc ttactatatc aacagcacct gtcctgcaat agtttgccat
    11521 gaaacctaaa tgccattgat aattcgtatt tccaacagta tcctgtatat ctgtcaaaat
    11581 tctgaaattc tttctcgcca gcctgtaaaa catacgtgtc cggtaagcag aactctcatc
    11641 attctcccaa tcaacattat agtacgattc attgccattg tatccataaa agggataagc
    11701 tttataacct gtgtatttta tgcccgtact aaaacgggtg cttggtaaaa gtgaatatga
    11761 aacatattca agtatatgtt ctgaactacc tcctgaataa cctgatacct gaaggaaaac
    11821 cgattgcaga taatccgggt aaacagaacc atcaccataa tcaaatgggt taataataat
    11881 tccgtaatat ataccaaggt ccgaatcata tgcaactgct ggtaaaaacc cgaagttcca
    11941 tcctttttta agtatgattt ccggctgttg ccctgctgat tcaagaagaa aaagcaaaaa
    12001 aggaaccagt aataaattct ttttcacctt tttactttat tattaataat ataaatgtag
    12061 tattttttta ttttttttgc ctgatataat ttttacctgc ttttaaatta aatacatgca
    12121 ttatatttga aaaaacatcc cgtaaaaatc gaaacctatg aaaaaaattc tgttactggc
    12181 agttatagca acgtttctta tttcaggctg caagcaaaaa gttatgaacg agaatccatt
    12241 tttaaacagg aagtataata ctccctttga agttcctcca tttgacaaaa taaaggcaga
    12301 gcatttcaaa cctgcgtatc tcaagggctt tgaggagcag aaggctgtca tcagggaaat
    12361 tattaacaat cccgaacctc ctacattcga aaacaccata aaggctttag aatatagcgg
    12421 caagcttctt ggggaggttt cacgggtttt cggggctcta aattcagccc acaccaacga
    12481 caccctgcag gcaataaata aggagattgc cccgctaatg gccaggcacc gtgacgatat
    12541 taatctgaac gatagccttt ttcagagaat aaaacaggtt tatgagaaca gggataaatt
    12601 taatctcacc gaagaagaaa agaagattct ggatgacact tacaagtcgt ttgtccgcaa
    12661 tggcgctgca ctatcgccgg aagataaaga gaaacttcgt aaaataaacg aggaactatc
    12721 gcttctttca gtgcgtttcg gagagaatct gcttgccgag acaaatgcct ttaagcttgt
    12781 actggaaaaa gaagaagaac tggttggtct gccggaaggt gtgagggctc aggcagctgc
    12841 actggcaaaa agcatggggc tcgacggtaa atatgtattt acacttcagg taccttcaat
    12901 gactccattc ctgcaatact ctgaacgcag agacctcaga gagaaactct ttacagctta
    12961 cttcatgaaa ggcgataatg ataatgagcg cgataacaaa gcaataatag cacgtatggc
    13021 aaaactaaga gttgaaaggg caaaactact tggatacgaa gattatgctt catacgtgct
    13081 tgaaagaaat atggcaaaga cacccgaaaa agtgtttgag ttgctgctgc agatctggga
    13141 tgctgcactt ccggtcgcaa aggcagaagc tgcagcccag caagcgatta tagacaggga
    13201 aggaggcaag ttcaaacttc agccgtggga ctggtggttc tataccgaaa aaataaaaaa
    13261 agaaaagttt gacctgaatg atgaagctac acggccatat tttcttacgg aaaatgttat
    13321 aaaaggaatg ttttatgttg ccaaccgcct ctataatctc gaattcaagg aaagaaacaa
    13381 cattccaaaa tatcaccccg acgtaaagac atatgaagtt acaagagaca ataagcatgt
    13441 gggagttctt tatattgaca attatcctcg tgaatcaaaa cgaggtggag catggtgcgg
    13501 tgcattcaga gggcagtaca gagaacccga cggaaaaatg gttacacctg tggtttatat
    13561 agtaaccaac tcaactcctc cctcaggcga caaaccttca ttacttactg tggaagaagc
    13621 aactacttta ttccatgaat tcggtcatgc tctccatcaa cttctttcaa attcaaccta
    13681 tcccggggtg tcaggcacct cagttcctcg cgactttgtc gaactccctt cccagataat
    13741 ggaacactgg gtactggaac ctgaagtgct caaagtgtat gcaaaacatt atcagacagg
    13801 cgaaataatt ccataggatc ttgttgataa acttgataag gcaagcaaat tcaatatggg
    13861 ctttattaca gttgaatacc ttgccgcttc acttcttgat atggagtacc atatacttaa
    13921 ggaacctgta gaactaaata tcagagaatt cgaaaagaaa gttctggata aatacggatt
    13981 aatccctgaa ataaaaccca ggtacaggtc gacatatttc aaccatattt gggcaggcgg
    14041 ttatgcagca ggctattaca gttatatctg gtgcgaaata ctggactgtg atgcattcca
    14101 ggcatttaag gaaactggtg acatctttaa cagagaggtt gcatcaagat ttgaaaaaga
    14161 gatactttca agaggcggta caagggaccc gcatgacatg tatctggctt tcagaggaaa
    14221 agaaccaagt gtagatgctt tacttgaaaa cagaggatta aaataatatt cataaactgt
    14281 taaaaatatt actgttatgc cagggctatc tcgtcttatg cctggtattg ccggtaaatg
    14341 ttagcattgc cggaaaagag cctgttgcag ttaagggagt acttgatctc agagaatata
    14401 aacctggtga caggataaag gttaacctga acggcgaatg ggaattctac tggaacaggt
    14461 tcctttatcc ttctgatttc acaacatctg aaaagaggcc acagcctgac atatatggaa
    14521 aagttcctgc ttactggaca agctattccg gggaaatacc ggttaataaa tacggatacg
    14581 cagcgtaccg ccttgttata cttctgccag aaaacactga aacatcatat gcattcgaca
    14641 ttcccgtttt cgactcttca ttcgaactct gggtaaacga ttccctgctt tactcaaacg
    14701 gcaagcccgg aacaaccagt gagacaacca tacccgaata cagacctgga ttaagagtat
    14761 ataattcttc atcagataca ttaagtattc tgattaacgt agcaaacttt caccaccgca
    14821 ggggtgggtt ctggcaaacc atggtattcg gaacctataa agacgtccag aaatcagtgg
    14881 ccagcaaatt ggctgccgat tatattacag ttggcatact tggaagcttt gccctgtttt
    14941 ttatgctatt cttcctgctc taccccagag acaaaattct cctaatttac agcctgaccc
    15001 tcctctttct cgctatcaga ccttttttca cttctgaata ccttatttat gattataccg
    15061 acgtgagctg gtcgtggacc atacggtatg aatacctttg ctcatttctc gctttaggcg
    15121 gttgggcatg gtataattac tacctctacc cctcgaaata taacaaagct ttctcacaat
    15181 ctcttaccgt actatttgta gcatctatta ttgccatact tgctacaagt gtcaaagtat
    15241 tcagctacag cgtatttatc ctttatattg gaatagtgtc acttgtagca catgctttga
    15301 tatcatcgtt caaaggaatc ctgaaaaaaa atataacaga tgcattttat ttcattctat
    15361 ttctgataat atctttcggc gccctgcatg atatttttgt ttctgcagga atgaccaaat
    15421 cttcatttgg ataccttatg ccatttttaa tggttatctt cctgtttgcc cagtcggtcc
    15481 ttcttctttc caaatggata aaagcattct acgaaaaaga aagactacag gttgaactcg
    15541 aaaaaataaa caggaatctt gaaaatattg ttgctgaaag aacccgggaa cttactttaa
    15601 gaactaaaga aatagaaaaa aagaacctca tgatagaggc tcagaatctg agactgactg
    15661 aaacaataaa tttcaagaat aaaatcttct cagttattgc tcacgacctg agaagtccgg
    15721 ttgtaaacat actttatatt ctcaatctgc tcaaagaaaa agaattcaaa gacaaatatg
    15781 aggaatttgc agattccagc ataaagtacg cccagatggt gattgctctt cttgaaaaca
    15841 tgcttgtgtg gggcagagga caggaagata aaatcaaata ttcacctggc atgcacgacc
    15901 ttgctgtgat gatccagacc aatatcagta tttttaaaga aacggcaaac aggaaaaaga
    15961 ttagcatcag tttcaatcag gaaggtaatt caatggcata ctgcgacaaa gacttgattg
    16021 atatcgtaat aagaaatctt ctgtcaaacg ctataaaata ctcgtatcca ggaggaatcg
    16081 taagcgttct tgtgaaggaa aacaaaaatg agaacactct atccgttaaa gtttgcgaca
    16141 acggaaccgg catccctgag gagcatctga aaaacctctt caatccgact gaaatcataa
    16201 gtacaccagg cactgagaat gagaaaggta caggcatagg actgaaactt tgccatgaac
    16261 taatgctgat taataaagga catatttctg ctgaaagcca tgaaggtagt ggctcatgtt
    16321 ttcagattgt gctgccttca agtcctgtgt tgctgtaaaa atttaacaga aatcctgaac
    16381 aattttttaa aaggtttgtt ttgtttgtga aaacaactca caaataatgt taagggatat
    16441 tatacgaatt gatgaagaac tttgcacagg ttgtggcttg tgcgttccta actgccacga
    16501 aggggctctt cagataattg atggaaaggt aaggcttatt agcgaactca tgtgcgacgg
    16561 gctgggggca tgaataggtc aatgttccga aggtgctata acaatcaaaa aaaagggaag
    16621 aataaaaaga atgttcaaaa tcaaaagtct caagacca
//
LOCUS       DAOSNB010000020        16643 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3498_length_16643_cov_5.177056, whole genome shotgun sequence.
ACCESSION   DAOSNB010000020 DAOSNB010000000
VERSION     DAOSNB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3498_length_16643_cov_5.177056"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..2410
                     /gene="metG"
                     /locus_tag="PLG42_01815"
     CDS             377..2410
                     /gene="metG"
                     /locus_tag="PLG42_01815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOU29977.1"
                     /translation="MKKFKRYLITSALPYANGPIHIGHLAGVYVPSDIYTRYLRLKGE
                     DVISICGSDEHGVPITLKARKEGITPQQVVDRYHEMNKKAFEDFGIAFDIYSRTTNKI
                     HYETASEFFLKLYNKGEFIEKTSLQYYDTEAGCFLADRYIIGECPHCHNENAYGDQCE
                     KCGSSLSPSDLINPRSAISGSQPVLKETLHWYLPLDKYEPWLKKWILEEHTEWKPNVY
                     GQCKSWLDQGLQPRAVSRDLDWGVPVPLKNASGKVLYVWFDAPIGYISATKELTPEWE
                     KYWKDPETRLIHFIGKDNIVFHCIIFPVMLKAEGSFILPDNVPANEFLNLENDKISTS
                     RNWAVWLHEYLEEFPGKQDVLRYTLCANAPETKDNDFTWKDFQARNNNELVAILGNFI
                     NRTLVLTNNYFSGRVPEPGILNNPDKEVLDEISRLTKATGEAIENFRFREGLKEAMNL
                     ARLGNKYLADTEPWKLIKTDPDRVKTIINISLQITANLSIILEPFLPFSMKRLREWIN
                     LSNFTWNDAGKTELLPPGHQVSKGGLLFEKIDDETINRQIEKLMKTRRENETVSAAAS
                     PLKEPVSYEDFSRMDIRTATVLEAERVPKTNKLLKLKIDCGTDIRTIVSGIAEYYEPE
                     TLVGKQILIIANLEPRKIKGIESRGMILMAEEPGGKLVLVSPVEKTDNGSVVK"
     gene            complement(2411..3394)
                     /locus_tag="PLG42_01820"
     CDS             complement(2411..3394)
                     /locus_tag="PLG42_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOU29978.1"
                     /translation="MDIVAIIRELLFSHDCVIIPGFGGFIGNYSPARTDKESCTFYPP
                     AKRISFNRNLNQNDGLLISRISVAANLNYNEAKELVEDFVADIKRRLTRGEKVTFERI
                     GTFVNNSEGNVQFEPDRDVNYNLNSYGLAPFQFPMLEGYDVRKRLMGDSYEDTVRSVS
                     IRRYLWRAAILVPLIGALIAVPLTTDIFKTKVQVTSLNPLITAISDSGVKEKPAVDET
                     RGLLSDSISEKIVDQVQSEQSTPAVNISEPVQQMPHEEYGIITGSFKSESNALLLVKK
                     LAGEGYSAEIIQGANGFYRVSALRCKSLSEAESVKNSMEARYPGAWIVKLK"
     gene            3505..4629
                     /locus_tag="PLG42_01825"
     CDS             3505..4629
                     /locus_tag="PLG42_01825"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU29979.1"
                     /translation="MKAIQMADLRAQYERLKPEIDKAINEVLLSTNFVKGPEIKKFED
                     ELAQYLGAKHVISCGNGTDALQIAYMALGLKPGDEIITTSFTFIATVETMALLGLKPV
                     FADVDEKTFNISPAEIRKRLTPSTRAIVPVHLFGQCADMEEILRIAEENSLYVIEDTA
                     QATGAEFIFSDGKRKKAGTMGIAGTTSFFPSKNLACYGDGGAIFTDDDELARKMRSIA
                     NHGMQERYHYECIGVNSRLDTIQAVILRVKLKYLDSFNEARQKAALFYDEAFASCPEI
                     IIPGRKENSTHIFHQYTIRVLNGKRDLLKKHLQSRNIPTMIYYPLPIHMQKAYRYLGW
                     NEGDLPVTEQLCKEVLSLPICPEIEREQLEYIAKSVLEFF"
     gene            4657..5235
                     /locus_tag="PLG42_01830"
     CDS             4657..5235
                     /locus_tag="PLG42_01830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU29980.1"
                     /translation="MEKEYFAHETAVIDEGCKIGKGTKIWHFSHIMTGCEIGEGCNLG
                     QNVVVSPGVKLGKNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIINPRSAIVRRDKYV
                     TTIVEKGATIGANATIICGNRIGRYAFVGAGAVVTKDVKPYALVVGNPARQIGWMSEY
                     GHRLNFDENGIAVCPESGEKYKLENGNVSKIC"
     gene            5262..6218
                     /locus_tag="PLG42_01835"
     CDS             5262..6218
                     /locus_tag="PLG42_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU29981.1"
                     /translation="MENTPKNFGLIGVAGYIAVRHLKAIKDTGNILLASLDKFDSVGI
                     LDNYFPNSDFFVEFERFDRHFEKLKRSGTMIDYVSICSPNYLHDAHIRFALRHKADAI
                     CEKPVVLNPWNIDALQEIEKETGKKVYSILQTRLHPAIKQLKEKIEKGPRNTKYDIDL
                     TYITSRGNWYFFSWKGDTQKSGGVATNIGVHFFDMLGWIFGKVQENIVHLSQYNKAAG
                     YLELEKARIRWFLSLDFNDIPEEFRAEGKRTYRSVTVDGEEVEFSEGFTDLHTASYAE
                     ILRGKGFGLEECRQSIEIVYSIRNAKPEGLKGNYHPMLKKIL"
     gene            6223..7512
                     /locus_tag="PLG42_01840"
     CDS             6223..7512
                     /locus_tag="PLG42_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOU29982.1"
                     /translation="MFDRLINKETKLSVIGLGYVGLPIALAFAKKLSVIGFDIKEDRI
                     QMMKKGIDPSNELPPEEFEGTDIYFSSNPDDLREASFHIIAVPTPIDKHNLPDLKPVL
                     SASETVGTILKKGDYVVYESTVYPGCTEEDCVPVLEKFSGLKYKEDFKVGFSPERINP
                     GDKNHTLTKIVKITSGCDAESAEEIARVYELIIEAGVYRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFNRMGINTYEVIEAASTKWNFLKFYPGLVGGHCIGVDPYYLSYKAK
                     ELGYHPQIIDSGRFVNDSMGRYVGKQTIKKIIANEKNPRDARILVMGITFKENVTDIR
                     NSRVVDVINEMKDFGATVEVVDPGASPEEVKKEYGIELATAPSGIYDAVVVAVSHKEY
                     LDLDENYFTSLLNSDGVLVDVKGIYRGKIKNIRYWSL"
     gene            7514..8542
                     /gene="galE"
                     /locus_tag="PLG42_01845"
     CDS             7514..8542
                     /gene="galE"
                     /locus_tag="PLG42_01845"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOU29983.1"
                     /translation="MKNLILVTGGTGYIGSHTVVELIEKGFEVVSVDSLHNSEAWIND
                     RIGMITGKTPPLEIFDLCEIDRLDNLFKKYPGISAVIHFAAFKAVGESVEKPLVYYRN
                     NILSTINILEMMKKHQVKYMVFSSSCTVYGQPDKLPVTEDSPLQPATSPYGNTKKICE
                     DIIKDTTVASNEIKAISLRYFNPIGAHPSALIGELPRGVPENLVPFITQTAIGLRDEL
                     KVFGNDYNTPDGSCIRDYLHVSDLAKAHVKAVERLIGGSNKKDYEIFNLGTGKGVSVL
                     EIINTFEKVTGIKLKYRITGRRAGDIEKIWADATLANKELGWKTEYSLGDALKSAWEW
                     EKNLRKMK"
     gene            8545..9603
                     /gene="rfbB"
                     /locus_tag="PLG42_01850"
     CDS             8545..9603
                     /gene="rfbB"
                     /locus_tag="PLG42_01850"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOU29984.1"
                     /translation="MKKTILVTGGAGFIGSHVVRRFVIKHPDYLIVNADKLTYAGNLE
                     NLADIEKFPNYIFEKTDIVDKKAVVDLFNKYQFDGVIHLAAESHVDRSIDSPDDFVMT
                     NVVGTVNLLNAALASWNSHFAGKLFYHVSTDEVYGTLGKKGSFTEKTPYDPKSPYSAS
                     KASSDHFVRAYFNTYKLPIIISNCSNNYGPNQFPEKLIPLMINNIKNNKPLPVYGKGE
                     NIRDWLYVEDHAAAIDLIYHKGGIGETYNIGGNNEWKNIDLVRLLCRIMDRKLGRKEG
                     SSEKLITFVKDRPGHDFRYSIDSSKLRNELGWQPSVSFEEGLEKTVDWYLENTRWLEN
                     VVSGEYEKYYEKMYLKRG"
     gene            complement(9898..11262)
                     /locus_tag="PLG42_01855"
     CDS             complement(9898..11262)
                     /locus_tag="PLG42_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOU29985.1"
                     /translation="MTNFYRKVKDTGNPACNISFSNHLLFSGIVLFTALFLFSCTEKF
                     TDIGINLLPSKDFVNIRSNDTITVESFTEYRDSVPTRNKTYSYLGGLYDPYFGSVSSD
                     FVAQLRLTQRWPSGGDNVIIDSVKLYLYISGAKGELGVDQIISLWEITEKLSSDSVYY
                     STRDPHAGYYIGSFALPRVEKDSAQTLQVSLPLSFGEYLLRDTTKLFQSGSGEDFRDF
                     FKGVYVTVKEAGESQGKGVIEGGRQLLILPFESGSSAVPFYITIYYHTYKASNLNFNF
                     IINNNSVRYNRYYHDFSSAEPEKRIVHINDGQKDTLSYLQAFYGVYTRIIMKGLSSFR
                     DSLPIAVNRARLSVPVFIDGEIYEKTNMPSPVYLIYKSKEGYNYIVPDYYMSSDFYNG
                     KFNSTTLSYTFNIAAFVQSYLEGKIPEPELIMSLGDNEYRNIIFKANTSNSPAKFELT
                     YTHY"
     gene            complement(11243..12049)
                     /locus_tag="PLG42_01860"
     CDS             complement(11243..12049)
                     /locus_tag="PLG42_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOU29986.1"
                     /translation="MESKKVLFVSQEITPYLAETRMSKISRYLPQGIQERGKEIRIFM
                     PRYGCINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNEDYFHR
                     KFVVTDSTGKEFEDNDERALFFARGVLETVKKLRWAPDIVHCHGWMSALVPVYLKDIY
                     HDDPIFANHKIIYSVYNNEFKTPFRSTFADKLRYDGIDGDNLKLAQKPDFINISKLAI
                     RNSDAVIIGSEDVNPELLKYIKSTGKPVLEYQDESNYIDAYNEFYDKLLS"
     gene            12205..13083
                     /gene="panC"
                     /locus_tag="PLG42_01865"
     CDS             12205..13083
                     /gene="panC"
                     /locus_tag="PLG42_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOU29987.1"
                     /translation="MKVFNTVKDLQHHLGSIKASPKGFVPTMGALHKGHFSLIEKSLL
                     ECPLTTVSIFVNPTQFNDKNDLERYPRTPQKDLSLLSEILRTDDAVFIPPVEEVYPAG
                     LKIENYDFGKLDKVMEGIYRPGHFNGVAQVVSRLFDIVKPDRAYFGQKDFQQLVIVRE
                     LVRKKGLPVKIIGCPIVREPDGLAMSSRNILLEPSLREKAGIIFKTLSDASEMLKDSD
                     IPEIKEFVASTINSTPGFKLEYFEIVDENELQPVSRKNSMLEDKKYFGCIAVWAGKIR
                     LIDNIEFRHPYSKKVH"
     gene            13122..13472
                     /locus_tag="PLG42_01870"
     CDS             13122..13472
                     /locus_tag="PLG42_01870"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOU29988.1"
                     /translation="MLIEVVKSKIHNVSVTDANLNYIGSITIDEDLMDAAGIIENEKV
                     QVVNINNGERFDTYVIKGTRGSGQICLNGAAARKVAIGDILIIISYASMTPEEAKNFK
                     PRILFPDTRTNKLV"
     gene            13475..14527
                     /locus_tag="PLG42_01875"
     CDS             13475..14527
                     /locus_tag="PLG42_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOU29989.1"
                     /translation="MSLKKSIIQLIKFFAFLIAGISLLWLAFRNINFSGLGQKIIHAD
                     YKWLVLSIIFATLAYISRARRWVLLIRPLGYNPSFKNTFNAMMTGYLANMALPRIGEI
                     TRCIALGKKEKIPTGQLFGTVIIERTVDMLSLLIITFIMLLIKGHLLGSFLVKNALEP
                     FTGKIKAASGSALILWIIILASILLISYLIFVYRKNLRKIRFFAKMFDISKGVVTGLK
                     TITSLREKGEFLFHTGFIWLNYALMSWIVVFTLESTSHLTFSDGFLLLVIGSLAMSAP
                     VQSGLGAFHFIISRGLNGLYGVSLEDGLAYALISHESQLIYGAILGFIAFYLLVKKDR
                     IKLTINGQNQNSVDLP"
     gene            14493..15857
                     /gene="radA"
                     /locus_tag="PLG42_01880"
     CDS             14493..15857
                     /gene="radA"
                     /locus_tag="PLG42_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOU29990.1"
                     /translation="MARTRTVWICRNCGSESVKWIGRCPSCGEWNTYQEETITPSTTS
                     RELKLAGDTGKRKPELLSEITGKEKERYKTGMSELDRILGGGLVAGSLVLLGGEPGVG
                     KSTLALQIALEKADRRVLYVSGEESGEQISLRARRIRKKNSECFILCETDLESIITHS
                     ENLKPEIIIVDSIQTISTPVLENVAGSVTQVRECAAQLLKYSKLSGIPVILIGHITKD
                     GSLAGPKVLEHIVDVVLYFEGENNYAYRVLRSVKNRFGSTSEIGIFEMTESGLREISN
                     PSELFINQHDEPLSGIAIAATIDGLRPFLIETQALVSSAVYGTPQRSSTGFDTRRMNM
                     LLAILEKRAGFKLGMKDVFLNIAGGLKVSDPAIDLAVISAILSSNLDVPVDRQLCFAG
                     EAGLTGEIRPVSRIEQRIREAAKTGFTGVVISKYQKDPFIKNAPLKIIQLGKIEHLVK
                     FLFS"
     gene            complement(15872..>16643)
                     /locus_tag="PLG42_01885"
     CDS             complement(15872..>16643)
                     /locus_tag="PLG42_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOU29991.1"
                     /translation="VGDSTWTPRSSGPREYHGKLVTLKWGLAQSENYISAWLMTQFTP
                     QAVVDLMHKMGIRSYIDPVYSIFLGTSDITLEEMVGAYSTFANKGVYTRPLYVTRIED
                     KNGNVISRFTTKVEEVITEDQAYLMLDLLQGVVNQGTAIRMRSVYGFKNQMGGKTGTT
                     QNHGNGWYIGVLPNLVGGVWSGWEDQSIHFETLGEGQGARIALPVMGLFLKKLYADTE
                     FGIMEADVFEKPAGFNIELDCNKVKRGTGRRSNYVERY"
BASE COUNT         5271 a         3440 c         3292 g         4640 t
ORIGIN      
        1 ttattttttt cagccgtggc ttttttaatt ttacagaaac aatgttacat aatctaaatt
       61 aataaataat tttcattaag gttattgtat tatttaataa tagcaaacta catactaatg
      121 ttatgcttta aattaaaatc aaaattactt aattatttgc ttttttcatc aagattatgt
      181 attcttctaa atcatagttt ctaagaaatt aaattataat tcgttatcag actacaagtt
      241 tgtaatataa ttttttgttc cacaagaact aatataaaga accttgtttg cttctatcac
      301 aattgttacg aataaatcct ctaattcaaa tttgttatat ttgcggagtt tttaataatc
      361 tgaacgtttt cgtgttatga agaaattcaa aaggtacctg attacttcag cattgccata
      421 tgcaaatggg ccaatacata tcggccatct ggcaggtgtg tacgtgccat ccgatatcta
      481 tacccgctac cttcgtctta aaggagaaga tgtcatctcc atctgtggat ctgatgagca
      541 cggtgtgcca ataaccctca aggcacgcaa agaaggaatt accccacagc aggtagtgga
      601 caggtaccat gaaatgaaca aaaaagcatt cgaggatttc ggaatcgctt ttgacattta
      661 ttcacgtaca accaacaaaa tacactacga aactgcttcg gaattcttcc tgaaactcta
      721 taacaaagga gaattcattg aaaagacatc acttcagtac tacgataccg aagccggttg
      781 ctttcttgcc gacaggtata tcataggaga atgcccacat tgccataacg aaaatgccta
      841 tggagatcaa tgtgagaagt gtggcagctc tctgagtccg tccgacctta tcaatccgcg
      901 ttctgcaatc agcggaagtc agcctgtgct taaagaaacc cttcactggt acctgccact
      961 cgataagtat gagccatggc tgaaaaaatg gattcttgaa gaacataccg aatggaaacc
     1021 caacgtttac ggccagtgca aatcatggct cgaccagggt cttcagccac gtgcagtaag
     1081 ccgcgacctc gactggggtg tgccagttcc tctgaaaaat gccagcggaa aagtgctata
     1141 cgtatggttc gatgcaccta taggctatat ctccgccact aaggaactta caccagaatg
     1201 ggaaaaatac tggaaagatc ccgaaacaag gctaattcat ttcatcggca aagacaacat
     1261 tgtgttccat tgcatcatat ttccggtgat gctcaaagcc gaaggatcat tcatattgcc
     1321 cgacaatgtg ccggcaaacg agtttctgaa ccttgagaat gacaaaatat ccacatcacg
     1381 caactgggca gtgtggctgc atgaatacct agaagaattc ccgggaaaac aggacgtcct
     1441 tcgatataca ctctgcgcca acgctcccga aacaaaagat aatgacttta catggaaaga
     1501 ttttcaggca cgcaataaca atgagctcgt tgctatactc ggtaatttca ttaaccggac
     1561 actcgttctt acaaacaact acttctctgg cagggtacct gaacctggta tattaaacaa
     1621 tcctgacaaa gaagtacttg atgaaatatc acggcttaca aaagccactg gtgaagcaat
     1681 tgaaaacttc agattcaggg aaggcctgaa agaggccatg aacctcgcac ggctaggaaa
     1741 caaatatctt gccgacactg aaccctggaa actaatcaaa accgatccgg atagagtcaa
     1801 aacaattatt aatatctcac tacagattac tgccaatctg agcatcattc ttgagccctt
     1861 ccttcctttt tcaatgaaga gactccggga atggatcaac ctcagcaatt tcacatggaa
     1921 tgatgccgga aaaactgaac tcctgccacc gggccatcag gtcagcaagg gaggactcct
     1981 gttcgaaaaa atcgatgacg aaactataaa caggcagatt gaaaaattaa tgaagacccg
     2041 cagggagaat gagactgtgt cagcagcagc atctcccctt aaagaacctg tttcttacga
     2101 agatttttca aggatggata tccgcacagc tacggtactg gaagctgaaa gagtgcctaa
     2161 aacaaacaaa ctgcttaaat tgaagattga ctgtggcacc gatatacgca caatagtatc
     2221 gggcattgca gaatactatg agcctgaaac actggttggg aaacagattc ttataatagc
     2281 caatcttgaa cctcgaaaaa taaaaggaat agaatcgaga gggatgatcc ttatggctga
     2341 agaacctggc ggtaaacttg tacttgtttc tcctgttgaa aaaaccgata acggaagcgt
     2401 ggttaaataa ctattttaac tttactatcc aggcacccgg gtagcgggcc tccatactat
     2461 tctttacaga ctctgcttct gaaagagatt tgcaccggag agcactcacc ctgtaaaatc
     2521 cattggctcc ctgaattatc tctgcagaat agccctcccc tgccagtttt tttaccagca
     2581 acaaagcatt tgactccgat ttgaaactgc cggttattat tccgtattct tcatggggca
     2641 tttgctgaac aggttctgaa atattgactg caggagtact ttgttcactc tgaacctgat
     2701 caacaatctt ctcacttata ctgtccgaca gcaaacctct tgtttcatct acggcgggtt
     2761 tctcttttac tccgctgtca gatatggctg ttatcagagg attgagactg gtaacctgaa
     2821 cctttgtttt gaaaatatct gtagtaagtg gcactgcaat caatgctcct ataagaggga
     2881 ctaagattgc agccctccag agatatcttc ttatacttac acttctgaca gtatcttcat
     2941 aagagtctcc catcaacctt ttacgcacgt cgtagccttc aagcatcggg aactgaaatg
     3001 gagcaaggcc atatgagtta aggttgtaat tcacatccct gtcaggctca aactgcacat
     3061 tcccttcact gttgtttaca aatgttccta tccgctcaaa tgtcactttt tctcctctgg
     3121 taagccttct tttgatatca gccacaaaat cctctacaag ctcttttgct tcattataat
     3181 ttaaatttgc tgcaactgat attctgctaa taagaagtcc gtcgttctgg ttcagattgc
     3241 gattgaatga aatgcgcttg gctggcgggt aaaatgtgca gctctcctta tctgtgcggg
     3301 caggtgaata attacctata aatcccccaa agcccggaat tattacacag tcgtgactga
     3361 aaagcagttc ccttataatt gcaactatgt ccacagaagt agatattgtt aaagcaaaat
     3421 taagaaaaat aaatcaatat ttcagcttta tgtttcaata agcagaattg tgctaacttc
     3481 gtgtttcaaa accattgata ttctatgaaa gcgatacaga tggccgattt acgggcacag
     3541 tatgagagac tcaaaccgga gatagataaa gccataaatg aagtgctact ttcaacgaat
     3601 tttgtcaaag ggccggaaat aaaaaagttt gaagatgaac tggctcaata ccttggtgca
     3661 aagcatgtaa tatcatgcgg caacggaaca gatgcactgc agatcgctta catggcactt
     3721 ggcctcaaac cgggtgatga aatcataact acaagcttca cctttattgc cacagtagaa
     3781 acaatggcgc ttctaggact gaagcctgta tttgccgatg ttgacgaaaa gacctttaat
     3841 atttcacctg ctgaaatcag aaaaagactc accccctcaa ctcgtgctat agttccggtg
     3901 catcttttcg ggcaatgtgc cgatatggaa gaaattttaa gaattgcaga agaaaatagt
     3961 ctttacgtta ttgaggatac agctcaggct acaggcgctg aattcatctt cagcgacgga
     4021 aaaagaaaaa aagcaggaac gatgggcata gcaggtacaa cttctttttt tccttcaaaa
     4081 aaccttgcat gctatggcga tggcggtgca atttttactg atgatgatga gcttgcccgt
     4141 aaaatgagaa gtattgcaaa tcatgggatg caggaaagat atcattacga atgcattgga
     4201 gtcaactcac gacttgatac cattcaggcc gtcatactcc gcgtaaagct taaatacctc
     4261 gatagtttca atgaagcacg gcaaaaggcc gctttatttt atgatgaagc ttttgccagc
     4321 tgcccggaaa taattatacc cggcagaaaa gaaaactcaa cacatatatt ccatcagtat
     4381 accatacgag ttctcaacgg aaaaagagat cttctgaaaa aacatctgca gtcgaggaat
     4441 atcccgacaa tgatatatta cccccttcct atacatatgc agaaagccta cagatacctg
     4501 ggatggaatg agggagatct gcctgtaacc gaacagcttt gcaaagaagt gctctccctg
     4561 cctatatgtc cagaaattga aagggaacag cttgaataca ttgcaaaatc tgttcttgaa
     4621 ttcttttagt ttttgattgt aaataaaaac aataatatgg aaaaagaata ttttgcccat
     4681 gaaacagcag taattgacga aggttgtaaa atcggaaaag gaacaaaaat atggcacttc
     4741 agccatatta tgacaggatg tgaaattgga gaaggatgta accttggtca gaatgtggtc
     4801 gtttcaccgg gagtaaaact tggaaaaaat gtaaaggtcc agaataacgt atcaatttat
     4861 acgggtgtta tttgtgagga tgatgttttc cttgggccct caatggtctt taccaatatc
     4921 atcaatccga gaagcgccat tgtaaggagg gataaatatg taaccacaat tgttgagaaa
     4981 ggtgccacaa taggagccaa tgccactatc atctgtggta accgcatcgg ccgttatgct
     5041 tttgttggag ccggagctgt tgtaacaaaa gacgtaaagc cttatgctct tgtggtaggc
     5101 aatccggcca ggcaaatagg atggatgagc gaatacggtc accgtcttaa ctttgatgaa
     5161 aatggcattg cagtatgccc ggaaagcggt gaaaagtata agctcgaaaa tggaaacgtt
     5221 tctaaaattt gttagtttta ctgcctgatt tttaatagtt gatggaaaat actccaaaaa
     5281 atttcggtct catcggtgtt gccggatata tcgctgtaag acacctgaag gctattaagg
     5341 atacaggcaa tatcctgctt gccagccttg acaagttcga cagtgtgggt attctcgaca
     5401 attacttccc gaacagtgat ttttttgtag aatttgagcg cttcgaccgg catttcgaaa
     5461 aactaaagcg ctcgggaaca atgatcgact atgttagtat ctgctctcca aattatctgc
     5521 atgatgctca tatcagattt gccctccgac acaaggccga tgcgatatgc gaaaaaccgg
     5581 tagtactgaa cccatggaac atcgatgctc tgcaggaaat tgaaaaggag accgggaaaa
     5641 aagtctactc aatcctgcag acccgccttc atcctgcaat aaaacaatta aaagaaaaaa
     5701 tagaaaaagg acctcgcaac acgaaatatg atatagacct tacttatatt acaagtcgtg
     5761 gaaactggta tttcttttca tggaaaggag acacccagaa atcaggaggc gttgccacca
     5821 atatcggggt tcattttttt gatatgcttg gctggatttt cggaaaagtg caggaaaaca
     5881 tagtccacct ttcacagtac aacaaagctg ctggttatct cgaactggaa aaggcgagaa
     5941 taagatggtt tctcagtctc gactttaatg acatccccga agaattcaga gcagaaggca
     6001 aacgcacata caggtcagta actgttgacg gtgaggaagt tgaattcagt gaaggattca
     6061 ccgacctcca cacagcatca tatgctgaaa tcctgagagg taaaggattc ggactcgaag
     6121 aatgccggca gtcaatcgaa atagtctatt cgattcgcaa tgcaaaacct gagggactga
     6181 aaggtaatta tcacccaatg cttaaaaaaa ttctataaga tgatatttga cagacttata
     6241 aataaagaaa ccaaactttc agtaattggt ctaggttatg tcgggctgcc aattgcactg
     6301 gcatttgcaa aaaagcttag tgtaataggc tttgacataa aagaagaccg tattcaaatg
     6361 atgaaaaagg gaattgatcc cagtaatgaa ctgcccccag aagaatttga aggcaccgat
     6421 atatattttt catccaatcc ggatgattta cgggaagcct ctttccacat tatagcggtc
     6481 ccaaccccta tcgataaaca taacctcccc gaccttaaac cagtactcag cgcttcggaa
     6541 acagtgggca ccatattgaa aaaaggagac tatgtggtgt atgaatccac tgtttatccg
     6601 ggctgtaccg aagaagactg tgtacctgtt cttgagaaat tctccggatt aaagtataaa
     6661 gaagatttta aagttggttt ctcccccgaa agaattaatc caggagataa aaatcatacc
     6721 cttacgaaaa ttgttaagat caccagcggc tgtgatgctg aatcggctga agaaattgca
     6781 agggtttatg agctgatcat cgaagctgga gtttacaggg caagttctat taaggtagct
     6841 gaagcggcaa aaatcatcga aaacacacaa cgcgacatca acatcgcctt catgaatgaa
     6901 ctctctatca tatttaacag gatgggcatc aatacttatg aagtaattga agctgcttca
     6961 acaaaatgga acttccttaa attttatcca ggccttgtgg gaggccactg tatcggtgtc
     7021 gacccttact atctttcata taaggcaaaa gagctgggat accatccgca gattatcgac
     7081 tcaggcaggt ttgtgaacga cagcatgggc cgttatgtag gcaaacaaac tataaaaaag
     7141 atcattgcga acgaaaaaaa cccaagagat gcacgcatac ttgtaatggg tattaccttc
     7201 aaggaaaatg tgactgacat tcggaattca agagtagtgg atgttatcaa tgagatgaag
     7261 gattttggtg caacagtgga ggtggttgac cctggtgcat cgccggagga agtgaaaaaa
     7321 gaatacggta ttgaactcgc cacagctcct tcgggcatct acgatgctgt ggttgtcgca
     7381 gttagtcata aagagtatct cgacctggat gaaaactact tcacatcatt gctgaattcc
     7441 gatggagttt tagtagatgt gaaaggcatc tacaggggaa aaataaaaaa tatcagatac
     7501 tggagtcttt aaaatgaaaa atcttatact ggtaacaggt ggaactggat atataggttc
     7561 tcatacagtg gttgaactga tagagaaggg ctttgaggtt gtatccgttg atagcctgca
     7621 taattcggaa gcctggataa atgaccgtat tggtatgata accgggaaaa cacctcctct
     7681 ggaaattttc gacctttgcg aaatagacag actcgacaat cttttcaaaa aataccccgg
     7741 tatatcagcc gtgattcatt tcgcagcatt caaagctgtt ggtgaatcgg tggaaaaacc
     7801 cctcgtatac taccgcaata acatcctctc cacaataaat atccttgaaa tgatgaaaaa
     7861 acaccaggta aaatacatgg tgttctcatc ttcatgcaca gtttacggac agcccgacaa
     7921 gcttcctgtt acagaagatt cgcccctgca acctgcaact tcaccttatg gtaatactaa
     7981 gaaaatttgt gaagatatca taaaagatac aactgtggct tccaatgaaa taaaggcaat
     8041 ttcactcaga tactttaatc caataggagc acatccttct gctctcatag gcgaactccc
     8101 cagaggtgta cccgaaaacc ttgtaccatt cattacacaa acagctattg gactgaggga
     8161 cgaactcaaa gtattcggaa acgattataa tacacccgac ggttcatgca tcagagatta
     8221 cctccatgtt tccgaccttg caaaggctca tgtaaaagct gttgaacgcc ttattggagg
     8281 cagcaacaag aaagattacg aaattttcaa tcttggcacc ggtaaaggag tttcagtcct
     8341 tgaaataatt aacacttttg aaaaagtaac agggataaaa ttaaaataca gaattaccgg
     8401 ccgccgagcg ggagatattg agaaaatatg ggccgatgcc accctggcta ataaagagct
     8461 cggctggaaa actgaatatt cccttggtga tgcccttaaa tcagcatggg aatgggaaaa
     8521 aaacctgcgt aaaatgaaat gaatatgaaa aaaaccattc ttgttactgg aggtgcagga
     8581 ttcataggat ctcatgtagt gaggcggttt gtaattaaac atcctgatta tttaatagtt
     8641 aatgccgaca agctgacata tgccggtaat cttgaaaatc ttgctgatat cgaaaaattt
     8701 ccaaattaca tattcgaaaa aacagatatt gttgataaaa aggctgttgt agacctattt
     8761 aataaatacc aattcgatgg agtgattcac cttgcagcag agtcgcatgt tgacaggtca
     8821 atagatagtc ccgacgattt tgtaatgaca aatgttgtag gtaccgtaaa cctgctgaat
     8881 gcagcccttg catcatggaa ttcacacttt gctggaaaac ttttttatca cgtctcaacc
     8941 gacgaagttt atggaacact tgggaagaaa ggttcattta ctgaaaaaac cccatacgat
     9001 cccaaaagtc cttactctgc ttcaaaagcc agttctgatc acttcgtaag ggcatatttc
     9061 aatacttaca aactgccgat aattatttca aactgttcaa ataattatgg cccaaaccag
     9121 tttcctgaaa aactcatccc tttgatgata aacaacataa agaacaataa accactgcct
     9181 gtatacggaa aaggtgaaaa catcagagac tggctttatg ttgaagatca cgctgcggca
     9241 atagatttaa tatatcataa agggggaatt ggtgaaactt ataacatagg tggtaataac
     9301 gaatggaaga atattgacct tgtgaggcta ctgtgcagaa taatggaccg taaacttgga
     9361 cgaaaggagg gttcttcaga aaaactgata acattcgtaa aggatcgtcc ggggcacgat
     9421 ttccgttatt ctatcgattc atccaaactc agaaatgaac ttggctggca gccttccgta
     9481 agtttcgagg aagggctcga aaagacagtt gactggtatc ttgaaaatac aagatggctg
     9541 gagaacgttg tctcaggtga atatgaaaaa tattatgaaa agatgtacct gaaaagaggt
     9601 tgatcctatt tatgccgaaa ttatgatttt ctccatcacc aataccaata actagagggc
     9661 tgtcttttct tgctgcaaat attttatcct gctcatatct gcacacaatt accagaccgt
     9721 aagctccttc aacgttgctt aatgctgaaa ttattgcctg ttcagctgta agatttcctt
     9781 caagatagag gtgctcaatc agattagcca ctgcttccgt gtcggtctgg ctggaaaatg
     9841 cttctccacg gccttccaga tgccttttta ttctggcata gaaaaaaatt aaaaaattta
     9901 atagtgggta taggttaatt caaattttgc aggcgaattg ctggtgtttg ctttaaaaat
     9961 tatatttcgg tattcgttat ctccaagtga catgatcagt tcaggttcag gaattttgcc
    10021 ttcaagataa ctctggacaa aagcagctat attaaatgtg taacttaaag ttgttgaatt
    10081 aaattttccg ttgtaaaaat cggaactcat ataataatcc ggtacaatat agttataacc
    10141 ttcttttgat ttatatatca aatataccgg cgaaggcata ttggtttttt catatatttc
    10201 tccatcgata aataccggca cactcagcct tgctctgttg accgcaatgg gcagtgaatc
    10261 cctgaaagaa ctcaggcctt tcattattat tctggtatat acaccgtaaa atgcctgtaa
    10321 atagctcaga gtatccttct gcccatcatt gatgtgaact attctctttt caggttctgc
    10381 cgagctgaaa tcatgataat acctgttata cctgacactg ttgttgttta ttataaaatt
    10441 gaaattaaga tttgatgcct tgtaagtatg gtaatatatt gttatataaa aaggaaccgc
    10501 tgaacttccc gattcaaatg gaagaatcag aagttgacgc cctccttcga taactccctt
    10561 cccctggctt tctccagctt cttttacagt tacataaaca cctttgaaaa agtcacggaa
    10621 atcttcacct gaaccactct gaaaaagttt tgtagtgtcc cttaaaagat attcaccaaa
    10681 cgaaagtggc aaacttacct gaagcgtttg tgctgagtct ttctcaaccc ttggaagagc
    10741 aaatgaaccg atataataac ctgcatgtgg atcccgggtt gaataataaa ccgaatcgga
    10801 actcagtttc tcagttatct cccagagact tattatctgg tctaccccaa gttcaccttt
    10861 tgcaccactt atatataagt aaagttttac ggagtcaata ataacattgt cgccacctga
    10921 aggccatcgc tgcgttaaac ggagctgggc aacaaaatcg gatgaaacac tgccaaaata
    10981 cggatcgtaa agtccgccca gataggaata ggttttgttc cttgtgggaa cactgtcgcg
    11041 gtattccgta aaagactcta ccgttatcgt atcatttgaa cggatattta caaaatcctt
    11101 tgaaggcaat aaattaattc ctatatcagt aaatttctca gtgcaggaaa acaaaaaaag
    11161 tgctgtaaac aatactattc cggaaaaaag caaatggttt gaaaaagaaa tattacaagc
    11221 cggattacct gtatctttta ctttacgata gaagtttgtc ataaaattca ttataggcat
    11281 caatgtaatt tgactcatcc tgatattcaa gaacgggctt gcctgtcgat ttaatatatt
    11341 tcaacagttc gggattaaca tcttcacttc ctataataac cgcatccgag ttcctgatgg
    11401 caagctttga tatgttaata aaatcaggct tctgagccag tttcagatta tccccatcta
    11461 taccatcata tctgagttta tctgcaaacg tagatctaaa cggagtttta aactcattat
    11521 tatatactga ataaataatt ttatgatttg caaaaatcgg atcgtcatga taaatgtctt
    11581 ttaggtatac aggtacaaga gccgacatcc atccgtggca atgaacaata tcgggggccc
    11641 accttaactt tttcaccgtc tcaagtaccc ccctggcaaa gaaaagagct ctttcatcat
    11701 tatcttcaaa ctctttacct gtactgtcgg tcacaacaaa tttcctgtgg aaataatctt
    11761 cattatcaat aaaatatacc tgcatccgcg ccgactgtat agatgccacc ttaataatca
    11821 gcggatgatc cgtatcgtct ataatcaggt tcattcctga taacctgatt acctcatgca
    11881 gctggtttct cctttcattt atgcatccgt aacggggcat aaaaattctt atttccttac
    11941 ccctctcctg aataccctgc ggaagatacc tgcttatctt cgacatcctg gtctctgcca
    12001 gataaggagt tatctcctgg gaaacaaata aaaccttctt actttccatc tattctgcca
    12061 ctataaaaac catcaatcgt ttcaggctgc aaatttagta aaaataattg atattaagta
    12121 tttttattaa ctttcgcacc ttccctgttt tttcttaggt ttgcatgtaa attcatcaca
    12181 tagaagaact tatttgccag taaaatgaaa gtattcaata cagttaaaga tctgcaacat
    12241 catcttggat ccataaaggc ctccccgaaa ggatttgttc ctacaatggg tgcccttcac
    12301 aaaggccatt tttccttaat tgaaaaatcc cttctggaat gtcccctgac tactgtgagc
    12361 atattcgtaa atccaaccca gttcaacgat aaaaacgatc ttgaaagata cccccgcaca
    12421 cctcagaaag acctttcatt gctttcagaa atactcagaa cagatgatgc tgtttttatt
    12481 cctccagtgg aagaagttta tcctgctgga cttaaaatcg aaaactacga tttcggtaaa
    12541 cttgataaag tcatggaagg aatttaccgg cccggtcatt ttaacggagt agcccaggta
    12601 gttagcagat tatttgatat agtcaaacct gatagagcat attttggaca gaaagatttt
    12661 cagcaacttg ttatagttag agaacttgtg aggaaaaaag ggcttcctgt aaaaataata
    12721 ggatgcccca ttgtgcgcga acccgacgga ttggcaatga gcagcaggaa tatcctgctc
    12781 gaaccatctt taagagaaaa agcaggaatt atttttaaaa cactttccga tgcctctgaa
    12841 atgcttaaag actcagatat accagaaata aaagagttcg tagccagcac aataaattca
    12901 accccaggtt tcaaactcga atatttcgaa atagttgatg aaaatgaact tcagcctgtt
    12961 tcccggaaaa attctatgct ggaagataag aaatactttg gctgtattgc agtatgggca
    13021 ggaaaaataa gactgataga taatatagaa ttcaggcatc cgtattccaa aaaggttcat
    13081 taactttgtg cctgattttt aatgttaaaa ccgggattga tatgcttatt gaggtagtaa
    13141 aatcgaaaat ccacaatgta tctgttacgg atgcaaatct taactatatt ggaagcatta
    13201 ccatcgatga ggatctgatg gatgcggcgg gaattattga aaacgaaaaa gtacaggtag
    13261 tcaatataaa caacggagag cgctttgata cttacgttat taaaggcaca cgaggttcag
    13321 gtcagatatg tctcaacggt gcagctgccc gcaaagtggc tataggcgat atattaataa
    13381 ttatctccta tgcttccatg acccctgaag aggcaaaaaa tttcaaaccc cgtattctct
    13441 tccccgacac ccgtacaaat aaacttgtct gaacttgagc cttaaaaaaa gtatcatcca
    13501 gctaataaaa ttttttgcat ttctgattgc aggcatctcc ctgctttggc tggctttcag
    13561 gaatataaac ttttccggac tgggacaaaa gattattcat gccgattaca aatggctcgt
    13621 actatcaatt atttttgcca ctttagcata tattagccga gcaagaagat gggtattgct
    13681 tataaggcca ctcggttata atccttcttt taagaacacc ttcaatgcca tgatgaccgg
    13741 ctatctggca aacatggcat tgccacgtat aggagagata accagatgta ttgccctggg
    13801 taaaaaggaa aaaataccaa ccggtcagct tttcggcact gtaataatag aacgtacggt
    13861 tgatatgctg tcactgctaa tcataacttt tataatgctt ttaataaaag gtcatttact
    13921 cggttcattt cttgtaaaaa atgcactgga acctttcact ggtaaaataa aagcagcatc
    13981 cggttctgca ttgatattgt ggataattat cctggcatca attttattaa tttcgtattt
    14041 gatttttgtt tacagaaaaa atctcagaaa gatccgattt tttgccaaaa tgtttgatat
    14101 atcaaagggc gtggttaccg gactgaaaac cattacctca ttaagggaaa aaggagaatt
    14161 cctttttcac actggtttta tctggttaaa ttatgccttg atgtcatgga tagtcgtttt
    14221 tacccttgaa agtacttctc atttaacttt cagtgacggt tttctcttgc ttgtaattgg
    14281 tagtctggca atgtcagcac ctgttcagag cggacttggt gcttttcatt ttattatctc
    14341 ccgcggactc aacggactct atggagtcag ccttgaagac ggacttgcat atgcactcat
    14401 ctcacatgaa tcccagctta tctacggtgc aatccttggc tttattgcat tttatcttct
    14461 tgtaaaaaaa gacagaataa aactgactat taatggccag aaccagaaca gtgtggattt
    14521 gccgtaactg tggatctgaa tcggttaaat ggatagggcg atgcccttcc tgcggcgaat
    14581 ggaatacata ccaggaagaa accattacgc cttcaacaac ctccagggaa cttaaacttg
    14641 ctggtgatac tggtaaacgc aaacccgaac tgctgtcaga aatcaccgga aaagaaaaag
    14701 agagatataa aacaggaatg tccgagctcg acagaatact gggtggcgga ttagtagctg
    14761 gctctctagt ccttctcgga ggggaaccgg gagtaggtaa atcaaccctc gccctccaga
    14821 ttgctcttga aaaggctgac agaagagtgc tatatgtgtc gggcgaagag agcggcgagc
    14881 agataagcct aagagcaaga agaatccgca aaaagaattc agagtgtttc atactctgtg
    14941 aaacagatct tgagagtata attacccatt cagaaaatct aaaacctgaa ataataatcg
    15001 tagattcaat ccagacaatt agcaccccgg ttctcgaaaa tgttgcaggt tctgtaacac
    15061 aggtgcggga atgtgcagcc cagctgctga aatattccaa attgtcgggt atccctgtta
    15121 tcctgatagg gcacataacc aaagacggtt cgctggcagg gccaaaagtt cttgaacata
    15181 ttgtggacgt tgttctttat ttcgaaggtg aaaataacta cgcataccgg gtacttcgat
    15241 ctgtcaaaaa ccgattcggt tcaacctctg agataggcat attcgaaatg acagaaagcg
    15301 ggctacggga aatatccaat ccttcagaac tcttcattaa tcaacacgat gaaccactga
    15361 gcggaatcgc aatagctgca acaattgatg gtctcaggcc ttttcttatt gaaactcaag
    15421 cacttgtaag cagtgcggtt tacggcactc cccagagaag ctcaacagga ttcgataccc
    15481 gacgaatgaa tatgttgctg gctattctgg agaaaagagc gggatttaaa ttggggatga
    15541 aggatgtatt cctgaatatt gctggtggcc ttaaagtatc agatccggca atagacctgg
    15601 ctgtaataag tgctatcctc tcgtctaacc ttgatgttcc tgtcgacaga caattgtgtt
    15661 ttgccggtga agccggactt acaggcgaaa tacggccagt ttcgaggata gaacaacgca
    15721 taagggaagc agcaaaaact ggattcacag gtgttgtaat ctcaaaatac cagaaagatc
    15781 cgtttattaa aaatgcacct ttaaaaatta tacagctggg gaaaattgaa caccttgtaa
    15841 aatttctctt cagctgaatc aggtcagccg attaatagcg ttctacataa ttcgaccgtc
    15901 tccctgtgcc cctttttacc ttgttacagt cgagttcaat attgaatcct gcaggtttct
    15961 cgaaaacatc ggcttccatt attccaaact ccgtatcggc atataatttc ttaagaaata
    16021 aacccattac tggtaaagca atccgtgcac cctgcccttc accaagagtc tcaaagtgaa
    16081 tactctgatc ctcccatcca ctccagactc cccctaccag attcggtaat acccctatat
    16141 accacccgtt accatggttc tgtgttgttc ctgtcttgcc ccccatctgg tttttgaagc
    16201 cataaaccga cctcatcctg atggcagtac cctgattgac cacaccctgt aaaagatcaa
    16261 gcataagata agcctggtcc tcggttatta cttcttccac ttttgtagta aacctcgaaa
    16321 taacattccc gttcttatcc tcaattcgtg ttacatacaa aggacgggta tagactcctt
    16381 tatttgcaaa tgtactgtaa gctcctacca tctcttcaag ggtaatatcg gaagtcccga
    16441 gaaatataga atagacaggg tctatataac tccttattcc catcttatgc atcaggtcaa
    16501 ctactgcctg aggtgtaaac tgtgtcataa gccatgccga aatgtagttc tccgactgtg
    16561 ccagacccca tttaagcgtc acaagtttgc cgtgatattc tctagggcca gaacttcttg
    16621 gagtccatgt ggagtctccc aca
//
LOCUS       DAOSNB010000021        16096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3659_length_16096_cov_4.589427, whole genome shotgun sequence.
ACCESSION   DAOSNB010000021 DAOSNB010000000
VERSION     DAOSNB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3659_length_16096_cov_4.589427"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLG42_01890"
     CDS             complement(<1..495)
                     /locus_tag="PLG42_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOU29992.1"
                     /translation="MALNYIWIAFFLIGFLVALGQLIFAGNTQIFNELVNAAFSNART
                     GFEISLGLTGALTLWMGLMKVGERGGVVSILAKLTAPLFRRLFPGLPSGHPAYGSIML
                     NIAANMLGLDNAATPVGLKAMSEMQEVNPKKDTASDAQIMFLVLNTSGLTIIPVSIMV
                     YRFQL"
     gene            630..1304
                     /locus_tag="PLG42_01895"
     CDS             630..1304
                     /locus_tag="PLG42_01895"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOU29993.1"
                     /translation="MARFSRIDVILKMRETGVVPIFYNKDTDICKNVIKAIYDGGIKV
                     FEFTNRGDFANEMFSELNRWASKELPDMILGAGSVVDAATAAIYIQAGANFIVSPILN
                     PEMAKVCNRRKILWIPGCGSLSEINQAEELGAEIVKIFPGLATGGPDFVKAVKGPCPW
                     TSIMPTGGVEPTLENLREWFEAGVTCVGIGSNLITKEIVQKKDWKSLTAKVNGVVKIA
                     KMFRIE"
     gene            1348..2004
                     /locus_tag="PLG42_01900"
     CDS             1348..2004
                     /locus_tag="PLG42_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOU29994.1"
                     /translation="MKSSVKIRLLTVLLSCSIVTVISAQDILLPPPVKQGGKPLMQAL
                     NERQTTRTFTGENLSLQQLSDLLWAAWGINRPEEKKRTAPSARNIQEIDVYVTLQSGL
                     YLYDAENNRLKQIHNRDIRSLTGTQDFVGKAPVNLVYVADMGKTGKKEGDKIADADLW
                     WSYANCGFIAQNVYLYCASANLGCVVRGLVPKEKLAPEMGLRSNQVILLAQTVGVPQK
                     "
     gene            complement(2016..3458)
                     /locus_tag="PLG42_01905"
     CDS             complement(2016..3458)
                     /locus_tag="PLG42_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOU29995.1"
                     /translation="MKYLASLLLFLLFAFCSDNKAPEPVGPLPSERQLAWHDLGYYMF
                     VHFSINTFTDKEWGYGDESPSLFNPSKLDCRQWAKVARDAGMKGIVITAKHHDGFCLW
                     PSKYTEHSVKNSPWREGKGDLIRELRQACDEYGLKLGIYLSPWDRNSSVYGTPEYITY
                     YRNQLRELLTEYGDIFEVWFDGANGGDGYYGGARETRRIDNRTYYDWPNTIQIVRDLQ
                     PNAVMFSDAGPDVRWVGNERGIGSLTNWCLLNRDEMYPGGPFAKILGQGHEDGKYWVP
                     AEVDVSIRPGWFWHAREDTMVKSPEKLLDIYYTSVGRNCNLILNVPPDKRGLIHENDV
                     KSLLGFKALLDREFANELARGKKVSASDTRGRKFSPANVNDGNPETFWATNDNVTSAY
                     ITIDLVKETEVNRILMQEYIKLGQRVQEFKVEALSDGSWKEVVKGTTIGYRIIRKFPT
                     IKASKIKVTILRSKACPVISNIELYRAPGD"
     gene            complement(3479..6871)
                     /locus_tag="PLG42_01910"
     CDS             complement(3479..6871)
                     /locus_tag="PLG42_01910"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU29996.1"
                     /translation="MKYLRLLTIGFIQLLLTINVSSQDLRTLETKVADLLAQMPARDN
                     NTTERLMNEMLSLGDAGLKMICNQVLPAGSGDDVRPRFAVESLSRHLSRYGLEAERAR
                     WEKICISFVESSKEYTVKDFFMKQLQLVGGEESVQAIKIYLTDKNLCSPAVGVISSVK
                     GATAEKILSDALSDPNLPCVASVMNALASMNSELAVNQFITWSQNKDIQIKAAAYRAL
                     AMSGSPLAYPVLSKAAREVAYKWEYSGATASLLNYANRVAEKGDITTMEKICKTLIGK
                     CNSRTNIQFKSGALETLVRYKGTDAMNYLLDAASHPDNSYRNKAIQLSLTLPGINVTR
                     KWMDYFPKAPAFSKPEIISMLGERKDETALPLIISTLGNPDPATRKEAVTALVKIKGQ
                     EAIPELITFMRNNKGAADQEAVRNVLMTVLNSKRMPQLLPVLETGPDEARRTVLELLA
                     WSRQPEYFKTVLQYVTSDNEPVRAAAYKALPELVSAGDQEKIIELLSLTDNPSYIADL
                     QLALSNAANQVKDPEKRSEIILRSPLLKTNKEKIIPVLANTGGREALKYVLDEFENGS
                     PEMREVCFKALTSWKDYTAASALFEICASGNKTYEAPAFNAYVRLVRSAPVTPEQRLL
                     LYRKIMPYALTAERKNQILSELGKIKTYQALFFVARYLDDPETSATAARAAMYIALPT
                     ADGKDGMYGTLVREILGKVITKLTGSEIEYERELIRKYLASMPPDEGFVPMFNGKDLT
                     GWQGLVGNPITRAKMSPAELAKQQAEANKKMLENWSVKDGCIYFSGKGDNLCSIKEYG
                     DFELLVDWKITKGGDSGIYLRGSPQVQIWDTSRTDVGAQVGSGGLYNNQKYPSKPLVV
                     ADNPVGEWNTFRILMIGEKVSVWLNGILVVDDVVLENYWDRSIPIFPRGPIELQAHGT
                     DLAFRDIYVREINEKVYNLTQEERDQGFVALFNGRNLDGWVGDKESYVVEDGMIVIKP
                     GKGSGGNLYTEKEYSDFIFRFEFQLTPAANNGVGIRAPLTGDAAYVGMEIQILDDTAP
                     VYANLQPYQYHGSVYGVIPAKRGYLKPVGEWNYEEITAKGTKIKVVLNGTVIVDGDIA
                     EAIEKGTMDGRNHPGLKNKSGHIGFLGHGSEVRFRNIRIKEM"
     gene            complement(6888..8150)
                     /locus_tag="PLG42_01915"
     CDS             complement(6888..8150)
                     /locus_tag="PLG42_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU29997.1"
                     /translation="MNKSGRRTFIKRAGVAFAAFTIVPRHVLGGKGYTPPSEQLTKGI
                     IGVGSMGRGHFNYEGTRLLAVCDVDKKHLEEAVALAGGKVDAYHDFRELLARTDIDIV
                     HIATPPHWHGIMAKMAAEAGKDIWCEKPMTRTIGEGIKVVEAVKKHGRMFRLNTWFRF
                     KDNFYGMGTTVKPLKKIVDSGVLGWPLKVTVSGITGFDWKFYWSGLHNLKPEPVPPEL
                     DYDFWLGPAPWKPYNPERVHSKFRGYWDYDGGGLGDMGQHYLDPVQYLLGKDNTSPVY
                     VEIDAPQQHWDAVGSWRRITYTYADGCKIVLDGENRDKDAAYIEGPYGKIFKGLKSDI
                     PNLQKLIDSLPDPEPQITTFTESVLTRKKFSLNEENGHRSCTIVNLGVIALRLGRNLK
                     YDPVKQRFVDDEGANRMINQPMREPWTI"
     gene            8622..8969
                     /locus_tag="PLG42_01920"
     CDS             8622..8969
                     /locus_tag="PLG42_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU29998.1"
                     /translation="MKINNNHEIESAEVQMEGAKKVRMKILIGPKDESHNIIMRHFTI
                     SPGGHTPYHQHNYEHVVKIEKNRGIIVDENGNEHEIKVGQSLFVKPGEKHQFKNPFSE
                     DFEFLCIIPNPQK"
     gene            complement(9117..9674)
                     /locus_tag="PLG42_01925"
     CDS             complement(9117..9674)
                     /locus_tag="PLG42_01925"
                     /inference="COORDINATES: protein motif:HMM:NF017238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU29999.1"
                     /translation="MEQHHLCPVWVGYLSLIPLRKLRHNPKKILGPYIKSGMTVMDYG
                     CAMGFFSIPMARLVGECGTVYCVDIQPEMLERLKKRAEKHGVSNIIRPLLVNSDYNPA
                     ELSGKLDFVMLFYVVHEVAEKKQLFADLAMMLKRGGKILFVEPEGHVTSDEFEESLKL
                     AAEAGLELTNDKPVRNKLSAILIKK"
     gene            complement(9681..10016)
                     /locus_tag="PLG42_01930"
     CDS             complement(9681..10016)
                     /locus_tag="PLG42_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU30000.1"
                     /translation="MEESPVNFEIGKVFRFAELVDYSEKSVVSRQVIKSERGSVTVFS
                     FDRGEGLSEHTAPFDALVQVIEGTARITIGGKEHILNAGESVIMPASVPHSVFAPERF
                     KIILTMIRG"
     gene            complement(10345..10956)
                     /locus_tag="PLG42_01935"
     CDS             complement(10345..10956)
                     /locus_tag="PLG42_01935"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOU30001.1"
                     /translation="MDYKKGIPLKRKRSYRDVKETYLIICQGENTEPVYFRSFKLTSA
                     DIKAISKPEFTNILRFVEEAQKIKNQYKNDYDNYWIVIDKDDNSDEDFNNAIALAKKY
                     GFKVAYSNQAFEFWLILHFICYIGPMHRKNYKKILDKYLGFSYNKKLSTCKKLYNALL
                     PFQGTAIKNAETVYSKIGDHTNIAAEESSTTVHELVKSLNRYL"
     gene            complement(10964..12211)
                     /locus_tag="PLG42_01940"
     CDS             complement(10964..12211)
                     /locus_tag="PLG42_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU30002.1"
                     /translation="MTAAPIREKFESFNQNKFNYNGKIQFLKSAVLYGANASGKTNFI
                     KALSFFKNFILTSANLNPEMKIPQIPFFLNIKTRTEPSEFEIVFLCCGLIYRYGFIIS
                     RETVIEEWLYQKDKRETMVFRRNSKGINIPRKYTILNELRTKNMIRPNALLLSIAAQF
                     NDKSSIEILQWFSNNLNIISGLTDELYSLFSINLLDDSKSKDIIISLIKTADPGIEDL
                     KVIEKEGENILFTLRAPTNQIPEIKKGKAVIKELNSVKHVINDSGEFETIVEFPFELF
                     ESEGTKKFFHLLGPVMDTLRYGKILVIDELDTKLHPLITRQIVKLFNNNKTNPNNAQL
                     IFATHDIQLLDAKIFRRDQIWFTEKQDDGSTRLYSLLDFKKGKTPRNDEKISKNYAYG
                     KYGAVPYTGDIDILFEDYINYNK"
     gene            12470..12820
                     /locus_tag="PLG42_01945"
     CDS             12470..12820
                     /locus_tag="PLG42_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30003.1"
                     /translation="MFEHKKNPLLPFPMFLVRMVRYFFYSVLLIAFSLMLGVMGYHFT
                     AGLGWIDSLYNASMILTGMGPVAELKTTTAKVFASFYALFSGIAFLSTVAVFFAPVVH
                     RFMHKMHLESESSG"
     gene            complement(12921..14072)
                     /locus_tag="PLG42_01950"
     CDS             complement(12921..14072)
                     /locus_tag="PLG42_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU30004.1"
                     /translation="MNTKISLSKILPVLMSFFVMSFVDLVGISVDRVSSDMDLSATLA
                     QLIPMATFLWFFLLSVPVGIIQARYGKRFVLNAGMVLTAAGLLIPFISYSFGMVLAGF
                     ALIGIGNTIVQVSANPLLLDVVPSNRASSFLSFSQFVKAVGSMIGAPLAAVFASQFGD
                     WKLEFLVFGIISLLAVIWLGSVKIEERKEEGYRATLKTSFGLLGNSYILMMVVAIFLV
                     VGVDVGFNSNSGQFLMKHFGIEQTAAESGRSVYFFGRMLGTFLGAIMLTRIQAPRFLM
                     WTTILSIASVAVLLVIPSKVAAWGLVFLTGLAVANIWPLIFSLTVGRFPERSSEISGL
                     MIMAVSGGAPIPFLIGWISDISNIVLGMSVLIVCLIYIFAVSVYNLKKI"
     gene            complement(14124..15059)
                     /locus_tag="PLG42_01955"
     CDS             complement(14124..15059)
                     /locus_tag="PLG42_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU30005.1"
                     /translation="MEKNIAIGVDIGGSHITSAAVDMTSGKVLKETMADRPVNNQAQA
                     FEIIDVWSSCLQEVMGHLKFDDIRGIGFAMPGPFDYVNGICYIKGVSKYENLYGFNIG
                     DAVKSKLELPDDFGIRFINDASAFAVGEAWAGKAKDYRKSMCITLGTGLGSAFISDRI
                     PVVDGPDVPKIGCVYHLPYGESIADDYFSTRWFIRSYKEATGKETGGVKELSDLAHGD
                     KTVQGLFMKFGNDLAVFLAPWLKKFGAEILVIGGNISNAYHLFGEVFEGRLRKESWKG
                     DVGISELKEDAALLGSAYLFDDHFWKAVQHALPLM"
     gene            complement(15278..>16096)
                     /locus_tag="PLG42_01960"
     CDS             complement(15278..>16096)
                     /locus_tag="PLG42_01960"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOU30006.1"
                     /translation="PTKLGFDPFELPVVSREYDSALHQVGTLGGGNHFIEIQKGDDGA
                     IWIMIHSGSRNIGKQVADYYNKKAISLNREAGEDYGPKTELAWLRLDSKIGQDYLKEM
                     QYCIEFAMCNRYLMMDRIIGVFNDYFGSKFSHGEIINIAHNYAAIEKHFGEELVIHRK
                     GATKATKGLKGIIPGSQGTRSYIVEGKGNPDSFESCSHGAGRVMGRNQAIRTLNLEEE
                     KRRLDKLDILHSIRTKNDLEEAPSAYKNIEEVMAQQADLVDVVTGLSPLAVIKG"
BASE COUNT         4599 a         3703 c         2989 g         4805 t
ORIGIN      
        1 cagctgaaac ctgtaaacca ttatactcac cgggattatt gtaagtcctg aagtattaag
       61 aaccagaaac attatctggg catcggaggc tgtatctttt ttgggattga cttcctgcat
      121 ttcactcatc gctttcaacc ccacgggagt agcagcattg tcgagaccca gcatattagc
      181 agcaatattc agcattattg agccataagc aggatgccct gaaggtagac ccgggaaaag
      241 ccttctgaaa agaggagctg ttaattttgc aagaatcgat actacgccac ctctctcacc
      301 aaccttcatc aatcccatcc ataaagtaag agcacctgtg agcccgagcg aaatctcgaa
      361 gcctgtcctg gcatttgaaa aggcagcatt aaccagctcg ttgaatatct gagtattacc
      421 agcgaatatc agttgcccga gcgccaccag aaagccaata agaaaaaatg ctatccagat
      481 atagttcagt gccataaaaa cacaataagg ggtgtaatgg cataaaggta aaaaaattct
      541 tttacctcag tccttaatat ctctctcctg tttaaatcct cttgctttta aaactatttt
      601 aggtacattt gtaacacaac attaaagtta tggcacgttt tagtagaatt gatgttattc
      661 tgaaaatgcg tgagacgggc gttgtgccca tcttctataa taaagataca gatatttgca
      721 agaacgttat aaaagctatt tacgacggtg gcataaaggt ttttgaattt accaaccgtg
      781 gcgattttgc aaatgagatg ttcagtgaac tcaacaggtg ggcttcaaaa gagttacccg
      841 atatgatact cggtgccggc tctgtggttg acgcagccac tgcagctata tatattcagg
      901 caggtgccaa ctttattgta tcgcctatcc tgaacccgga gatggcaaaa gtctgcaaca
      961 ggcgaaaaat tctctggata cccgggtgcg gttcgctgtc tgaaataaac caggctgaag
     1021 aacttggcgc tgagatagtg aagatatttc cgggacttgc taccggcgga ccagattttg
     1081 taaaggcagt caaaggccca tgcccatgga caagcattat gcctaccggc ggtgtggaac
     1141 ctactcttga aaacctcagg gaatggttcg aagccggtgt tacttgtgtg ggaataggct
     1201 ccaaccttat aactaaggaa attgtacaga agaaagactg gaaaagtctc acggcaaaag
     1261 taaacggagt ggtgaaaatt gctaaaatgt tcaggataga gtagtagatt ttctgagcat
     1321 atctaacatt tcataaattt taaagccatg aaaagttcag ttaagatcag acttttaaca
     1381 gtacttctgt cctgttctat tgttacagta atttcagcac aggacatcct tctgcctcca
     1441 cccgttaaac agggcggaaa acctctgatg caggcactta atgaacgtca gacaacccgc
     1501 acttttacag gcgaaaatct atcactgcag caactatcag accttctctg ggcagcatgg
     1561 ggtatcaatc ggcctgagga aaagaaacgc actgccccat ccgcaagaaa tattcaggaa
     1621 atagatgtat atgtaaccct gcaaagcgga ctctatcttt acgacgctga aaataacaga
     1681 cttaaacaga ttcataaccg cgacatccgt agcctgacag gcacccagga ctttgtaggt
     1741 aaggcacccg ttaaccttgt gtatgtcgct gatatgggca aaaccggaaa aaaggaaggg
     1801 gataaaattg ctgacgcaga cctctggtgg tcgtatgcaa actgcggatt tatagcacag
     1861 aatgtttacc tctactgtgc ctctgccaac ctgggttgcg tggtgcgcgg acttgttcca
     1921 aaggagaaac tggcaccaga gatgggtctg agatcaaatc aggtgattct ccttgcacaa
     1981 acagtaggag tgccccaaaa ataaatacga taaactcaat cacccggcgc cctgtaaagt
     2041 tctatattgg aaattaccgg gcaggcttta gatctcaata tagttacctt aatctttgaa
     2101 gctttaatgg ttggaaattt tcttattatc ctgtaaccta ttgttgtccc ttttacaact
     2161 tctttccagc ttccatcgga tagcgcttca actttaaatt cctgtaccct ctgtcccagc
     2221 tttatatact cctgcataag aatacgattc acctcggttt ctttaactaa atctatagtt
     2281 atatatgctg aagttacatt atcatttgtt gcccagaagg tttcaggatt accatcatta
     2341 acatttgctg gtgaaaactt ccttcccctt gtatctgagg cagagacctt cttcccacgg
     2401 gcaagctcat ttgcaaattc cctgtcgaga agtgccttaa atcccagaag cgattttaca
     2461 tcgttttcat gaataagccc tctcttatcg ggtggcacat tcagtatcag gttgcaattt
     2521 ctgccaacag acgtatagta aatatccagc agtttttcag gcgactttac cattgtatct
     2581 tctctggcat gccagaacca gccgggtcgg attgagacat ccacttctgc cggaacccag
     2641 tatttaccat cttcatgacc ctggccgagt atctttgcaa aaggtccgcc cggatacatc
     2701 tcgtccctgt tcagcaaaca ccagtttgtc agactgccta tccccctttc atttccaacc
     2761 caccgcacat cagggcctgc atcactgaac attactgcat tcggttgcaa atctctcact
     2821 atctggatag tattgggcca gtcgtagtaa gtgcggttgt caatacgtcg tgtttccctc
     2881 gcccctccat aataaccatc tccgccgttt gcaccatcaa accacacttc aaaaatgtca
     2941 ccatattccg taagcagttc acgcagctgg ttacggtaat aggtgatata ttccggcgtg
     3001 ccataaactg aactgtttct gtcccacggc gaaagataaa ttcccagctt caaaccatat
     3061 tcatcacatg cctgacgcag ttcccttatc aggtcgccct tgccttcacg ccatggagaa
     3121 ttttttaccg aatgctccgt atacttcgaa ggccagaggc agaaaccgtc gtgatgcttg
     3181 gcggttataa ctatcccctt catacccgca tccctcgcca cctttgccca ctgcctgcaa
     3241 tcaagcttcg acggattgaa aagtgacggc gactcatcac cgtaacccca ttctttatca
     3301 gtaaaagtgt tgatagaaaa atgaacgaac atataatacc cgaggtcatg ccaggcaagc
     3361 tggcgctctg aaggcaaagg accaacaggt tcaggtgctt tgttatcaga acaaaacgca
     3421 aataacagaa acaacaataa ggaagctaaa tatttcatat ttaatagaat aaatttctct
     3481 acatctcttt tattctaata ttgcgaaatc ttacttcaga gccgtggccc aaaaaaccaa
     3541 tatgtcccga cttgtttttc aaaccgggat gatttcttcc gtccatagtt cctttttcta
     3601 ttgcctcggc aatgtcgccg tcgacaatca ccgtaccgtt gagcacaacc ttaatttttg
     3661 ttccctttgc tgttatttct tcataattcc attccccaac cggcttcaga tagcctcttt
     3721 ttgcaggaat gactccataa accgagccat ggtactgata cggttgcaga ttggcataga
     3781 ccggtgctgt atcgtcgaga atctgaattt ccataccaac atatgcagca tcgcctgtta
     3841 gcggtgcacg tattccaacg ccattgttgg ctgccggggt aagctggaac tcaaatctga
     3901 aaatgaaatc agaatactcc ttctctgtat aaagattgcc tccgcttcct tttcctggct
     3961 taatgacaat catcccatct tcaacaacat acgattcctt atcgccaacc catccgtcaa
     4021 gatttctgcc gttgaaaagt gctacgaagc cctgatccct ctcttcctgt gtgaggttgt
     4081 aaactttttc atttatttct cttacataga tatcacggaa tgctaggtct gtgccgtgag
     4141 cctgaagctc gataggtcct ctcggaaaaa taggtattga acgatcccag taattttcca
     4201 gaacaacatc atcaacaaca agtattccgt tgagccaaac agaaactttt tcgcctatca
     4261 tcagtatcct gaaagtattc cattcaccaa caggattatc agccaccaca agcggttttg
     4321 aaggatattt ctgattgttg tagagtccgc ccgaaccgac ctgggctcct acatctgtcc
     4381 ttgaagtatc ccatatctga acctgcggtg aacctctgag ataaatgcca ctgtcgccgc
     4441 cttttgtgat tttccagtca acaagtaact caaaatcacc gtattcctta attgagcaga
     4501 gattatcgcc tttcccgctg aaatagatgc atccatcttt aacactccag ttctcaagca
     4561 tcttcttatt tgcttcagcc tgctgttttg ccagttcggc aggtgacatc tttgcacgtg
     4621 tgattggatt tccaaccaga ccctgccagc cggtaaggtc ttttccgttg aacattggca
     4681 caaatccttc atcgggaggc atcgaagcaa ggtattttct gatgagttcc ctttcgtact
     4741 ctatctctga acctgtaagt tttgtaataa cttttcctag tatctctctt acgagtgtgc
     4801 catacattcc atcttttcca tcggctgtcg ggagtgcaat atacatggca gcacgtgcag
     4861 cggtagcgct cgtttcaggg tcatcgaggt agcgggcaac gaaaaagagt gcctgatatg
     4921 ttttaatttt acccagttct gaaaggatct ggttcttccg ctctgcagtc aaagcatacg
     4981 gcattatctt gcggtaaaga agaagtctct gttccggagt aacaggagcc gacctcacca
     5041 gcctgacgta agcattgaaa gcaggcgcct catatgtctt gttgccagag gcacaaatct
     5101 caaaaagcgc agaggctgca gtataatctt tccatgaggt gagagcttta aagcatacct
     5161 ccctcatttc gggagaacca ttttcaaatt catcaagcac atatttcagg gcttctcttc
     5221 cgcctgtatt tgcaagaacc ggaataattt tttctttgtt ggttttcaga agaggggaac
     5281 gcaaaataat ttccgacctt ttttcggggt ctttcacctg atttgcagca tttgaaagcg
     5341 caagttgaag gtcggcaata tatgaaggat tatctgtgag agatagcagt tcaattattt
     5401 tttcctgatc gcctgctgaa acaagttcgg gaagagcttt gtatgctgca gcccttaccg
     5461 gctcattatc agaagtgaca tactgaagaa ctgttttgaa atactcaggc tgacggctcc
     5521 atgccagtaa ctctaaaaca gtcctgcgcg cttcatcagg tcctgtttca agtaccggca
     5581 gaagctgggg cattctcttt gaattaagaa ctgtcataag tacattcctg actgcttcct
     5641 gatctgcggc tcccttatta tttctcatga atgtgataag ttcaggaatg gcttcctgtc
     5701 ccttaatctt tacaagtgca gttacagcct cctttcttgt agcaggatca gggttgccga
     5761 gtgttgaaat tatcagagga agggcagtct catccttcct ttcgcccagc attgaaataa
     5821 tttcgggctt tgaaaatgcg ggagctttcg ggaaataatc catccatttt cgcgtgacat
     5881 ttatccctgg aagggtaagc gaaagttgta ttgccttgtt tctgtagctg ttatccggat
     5941 gcgaagccgc atcgagcaga tagttcattg catcggtgcc tttatacctg acaagtgttt
     6001 cgagggcacc tgatttgaac tgtatgtttg tccgggagtt gcattttccg ataagagttt
     6061 tacatatctt ttccatagtt gttatatctc ccttttcagc cactctgttg gcataattga
     6121 gcagtgatgc tgttgctccc gaatattccc atttgtatgc tacttctctg gcagctttac
     6181 tcaggaccgg gtaagccagg gggctcccgc tcatggcaag tgcccggtat gcagctgcct
     6241 taatctgaat atccttattt tgtgaccatg tgataaattg atttacagca agttctgaat
     6301 tcattgaagc aagtgcattc atcaccgatg caacacatgg caaatttgga tccgaaagag
     6361 catcagaaag tattttttcg gctgttgctc cttttaccga agagataact cccacagcgg
     6421 gtgagcagag attcttatcg gtaagataaa tctttattgc ctgtactgac tcttcacctc
     6481 ctaccagctg aagctgtttc ataaagaaat cttttacagt atactctttt gaagactcaa
     6541 caaagctgat gcatatcttt tcccatctag ccctttctgc ctcaagccca taacgggaaa
     6601 gatgacgtga aaggctttcc acggcaaacc tcggtctgac atcatcaccg cttccggcag
     6661 gaagaacctg attgcagatc attttcaaac ccgcatcacc cagtgagagc atctcattca
     6721 tcagcctttc ggtggtattg ttatcccttg caggcatctg ggcgagaagg tcagcaactt
     6781 ttgtttcaag tgtacggaga tcctgggaac tgacatttat tgtcagcaaa agctggataa
     6841 atcctattgt taataatctt aaatatttca tattcaatta tttttcatta aatagtccat
     6901 ggttcgcgca ttggctggtt gatcatacgg tttgcacctt catcgtcaac aaacctttgt
     6961 tttacagggt cgtatttaag gtttcgtccc agtcgcagag ctataacacc gaggttgact
     7021 attgtacacg acctgtgtcc gttctcctcg ttaagggaaa attttttcct tgtaagtacc
     7081 gactcggtaa aggttgttat ctggggttca gggtcgggca ggctgtcgat tagtttctgc
     7141 agattgggta tatccgactt caggcctttg aatatttttc catatggtcc ttcaatatat
     7201 gcggcatctt tgtcgcggtt ttcgccatcg agaactattt tacaaccgtc ggcgtaggta
     7261 taggttattc ttcgccatga gcccacggca tcccagtgtt gctggggtgc gtcaatctct
     7321 acataaaccg gactcgtatt atcctttcca agcaggtatt gaaccggatc gagatagtgt
     7381 tgtcccatgt cgccgagtcc tccaccatcg tagtcccagt aacccctgaa tttcgagtgt
     7441 acgcgttcag ggttataagg tttccagggg gcagggccga gccagaaatc gtaatcaagt
     7501 tcaggtggaa caggttcagg tttaaggtta tggaggcccg accagtagaa tttccagtcg
     7561 aagccagtga ttccgcttac ggtcacctta aggggccagc ctaaaacacc gctgtcaaca
     7621 attttcttga gaggtttgac tgtggtgccc atgccataga aattgtcttt gaatctgaac
     7681 caggtgttga ggcggaacat gcgtccgtgt ttttttacag cttcaacaac ctttatcccc
     7741 tcgcctatgg ttcttgtcat gggtttttcg caccatatat ctttccctgc ttcggcggcc
     7801 atttttgcca ttataccatg ccagtgcggc ggggtggcaa tatggacgat gtcaatatcg
     7861 gtgcgggcaa gtaactcacg gaaatcgtgg tatgcgtcca cctttccccc ggcaagggct
     7921 actgcttcct caagatgttt tttgtcgaca tcacatacag caagtaaacg ggtaccttcg
     7981 taattaaagt gccccctgcc catgcttccc actccaataa tgccttttgt aagttgttcg
     8041 ctgggagggg tatagccttt cccacccaat acatgacggg gaacaatggt aaaggccgca
     8101 aatgcaaccc ctgccctctt tatgaatgta cgtctgcctg atttattcat gattaaactg
     8161 gtttgactca aaaataagta aaaagaagaa gtattaaagg taatttcaat tttttaacat
     8221 gagaaaaatc aagatgggtt aatgtttgtt ttttgctcaa agcagcattc aatggtcact
     8281 cgattgtcat ttgcgcttca ctggactcag aggggattcg atagtaattc aatagtaatt
     8341 cgatagtaat tcaattgttc ccgatatgtt gcaatatcgg gacctccgcg gaatttatcc
     8401 cccaaagcag gacttcaaaa ttaaattgtt cccaatatgc tgagccagcg ggaatgtcat
     8461 cctgtatacc gggtctctaa aattcggatg ttttttcatc ttattatcgg taacatcatc
     8521 gaaactgttc ccagaattca ttaaacattt acaaactttc atatgtttgt atattaaaat
     8581 gcaggaaaaa attgatttat aaataataaa accatctgaa tatgaaaata aataataacc
     8641 acgaaattga atctgcagag gtgcagatgg aaggcgcaaa gaaagtcagg atgaaaatcc
     8701 tgattggccc gaaagatgaa tcccataata ttataatgag acatttcaca atctcaccgg
     8761 gtgggcatac cccttaccat caacacaact atgaacatgt ggtcaagatt gaaaaaaaca
     8821 ggggaattat tgtcgatgaa aacggaaatg aacatgagat taaagtaggt cagagccttt
     8881 tcgtaaaacc cggtgaaaaa catcagttca aaaacccctt cagtgaggat tttgaatttc
     8941 tgtgtatcat tcccaacccg cagaagtaaa cccgaatctt cctctggcta cataaaagat
     9001 tactattttt ccggctatcg cacggaaatt tcactttttt tattattcat atcaaaaaat
     9061 ctattgatta acccggaaag tcagaaatgt tgtttaatca actaagtttt tagatattat
     9121 ttttttatca atattgctga aagcttgttt cttacaggtt tatcgtttgt taattccagc
     9181 ccggcttccg cagccagctt cagcgattct tcaaactcat cagatgtgac atgtccctct
     9241 ggctcaacga ataagatttt cccacctctt ttaagcatca tggcaagatc ggcaaacaat
     9301 tgtttctttt ctgccacctc atgtacaacg tagaataaca ttacaaaatc gagtttcccg
     9361 gaaagttccg ccggattata atctgaatta acaagcaacg gccttataat attgcttacc
     9421 ccatgcttct ccgccctttt cttaagtctt tcaagcattt caggctggat atccacacaa
     9481 taaactgtgc cacactcccc taccagtcga gccataggaa tgctgaagaa acccatggca
     9541 caaccatagt ccatcacagt catgccagat ttaatatatg gtccaagtat ttttttggga
     9601 ttatgccgaa gtttgcggag aggtataagt gataggtacc ctacccatac cggacaaaga
     9661 tggtgttgtt ccataattta tcaacccctt atcatcgtca gaataatctt gaaccgctca
     9721 ggtgcaaata ctgaatgtgg cactgatgcg ggcatgataa ccgattcccc ggcattaagt
     9781 atatgctcct tgccgcctat tgttatcctt gctgttcctt ctatgacctg aacgagagca
     9841 tcaaacggtg cagtatgctc actcaaaccc tccccccgat caaaggaaaa tactgttacg
     9901 ctgccacgct ccgactttat tacctgacgg cttacaacac tcttttctga ataatctact
     9961 aattctgcaa acctgaaaac ctttccgatt tcaaaattca ccggtgattc ttccatgact
    10021 tattttttga ttgttttcat gaagttaaca ttaaaccaga gcttttgttc agggtgggtc
    10081 attaaaagat tttacacttt gaattcaaaa tacaattgaa tggcacacgg atgacacgga
    10141 tgaaacagat agccacggat atcagaaaat taattcaagt ttatctgagt actgatggaa
    10201 tgcttaaatt aaatatcaaa ttaaaggttt ctcatgtaat caagcctgct gaatttggga
    10261 tacaaaccag aacttaatca tattctaaca gttgctgatt taggggaaaa ggggttattt
    10321 aaaaaaatca ttcatcataa agtattataa atatctatta aggcttttaa ccagttcgtg
    10381 tactgtggtt gaggattctt ctgcagcaat atttgtgtga tctccaattt tgctgtagac
    10441 agtctcagca ttttttattg cagtgccttg aaatggtagc aaagcattgt acaatttttt
    10501 gcaagtagac aattttttat tataagaaaa tcctaggtat ttgtccagaa tttttttata
    10561 attctttctg tgcattggac caatgtagca aataaaatga aggataagcc agaattcaaa
    10621 agcctgatta ctataggcaa ctttaaatcc gtattttttc gccagagcta ttgcattatt
    10681 aaaatcttca tcagaattat catctttgtc tattacaatc cagtaattat cataatcgtt
    10741 tttatactga ttttttattt tctgagcttc ttcaacaaac cttaaaatat ttgtaaattc
    10801 aggctttgat atcgccttta tatcggcact tgttaatttg aatgacctga aatatacagg
    10861 ttcagtattc tcaccctgac agattatcaa atacgtttct tttacatccc tataacttct
    10921 ttttcttttt agcggaatcc cctttttgta atccattcaa aatctattta ttgtaattaa
    10981 tatagtcttc aaaaagaata tctatatccc cagtataagg aaccgcgccg tattttccat
    11041 aagcataatt tttacttatc ttttcatcat ttcttggagt tttgcctttt ttaaaatcga
    11101 gaagtgaata aagtcgggtt gaaccatcat cctgtttctc agtaaaccat atctgatctc
    11161 tcctgaaaat tttggcatcc aaaagttgta tatcatgtgt ggcaaaaatt aactgggcat
    11221 tattagggtt tgttttatta ttgttgaata attttactat ttgccgggtt attagaggat
    11281 gaagcttggt atcaagctca tcaataacaa gtattttacc ataccgaagt gtgtccataa
    11341 ccggacccag caaatgaaaa aatttttttg taccctcaga ttcgaacaat tcgaaaggaa
    11401 actcaacaat tgtttcaaat tctcctgaat cattaataac atgtttaaca ctgttcaact
    11461 ctttaataac tgccttacct tttttaatct caggtatctg attagttggt gcccttaggg
    11521 taaataaaat gttctcacct tctttttcaa ttactttaag atcttcaatt ccgggatcag
    11581 cagttttaat gagagagata ataatgtctt ttgatttaga gtcgtcaagc aagtttatag
    11641 aaaaaaggct ataaagctca tccgtaagcc ctgaaataat attaagatta tttgaaaacc
    11701 actgcaatat ttctatagaa cttttatcat taaactgagc ggctatcgaa agaagcaggg
    11761 cattaggtct gatcatattt tttgttctca actcattaag aattgtatac ttccggggaa
    11821 tattaattcc tttactgttt cttctaaaaa ccattgtttc tctcttatcc ttctgataca
    11881 accactcttc gattactgtt tccctgctaa ttataaaacc atacctgtat ataagtccgc
    11941 agcataaaaa aacaatctca aactcagatg gttctgttct ggtttttata ttaagaaaaa
    12001 aaggaatttg tggtattttc atttccggat taagattggc agatgttaaa ataaaatttt
    12061 taaaaaaact taatgcttta atgaaatttg tttttccact ggcatttgca ccatataata
    12121 cagctgattt aagaaactgt atcttaccat tataattaaa tttgttctga ttaaatgatt
    12181 caaatttctc acgaatcgga gccgctgtca tttgaaaaat ctgtgtttct ttaaatgaca
    12241 gaaaattttt aatagaaaaa ccaattatca tatttgtaat atttgtgata tcttcacaaa
    12301 aataataatt aagtattaaa aacaaatagt tcagctaatt tatttttaaa aagtgcctta
    12361 taaaataata aataattccc ggtgcatgtg tttaacagat catggctcaa ttataaatct
    12421 ccaggaataa accctaactt agcacccaaa gctataaaac cttagagcca tgtttgaaca
    12481 taaaaaaaat ccgcttttgc cttttcccat gtttcttgtc agaatggtga ggtacttttt
    12541 ttattcggta ctactgattg ccttctctct tatgctggga gtcatgggtt accattttac
    12601 ggccggactc ggatggatcg actcgctgta caacgcatca atgatcctta ccggtatggg
    12661 tccggtagcg gaacttaaaa caaccactgc caaagtgttt gcatcatttt atgccctctt
    12721 cagcggtata gcattcctga gcaccgttgc cgtcttcttt gctccggtag tgcatcgctt
    12781 catgcataaa atgcacctcg aaagtgaatc ttcaggataa gaattattat tatacctgaa
    12841 aaattacagc tatccccctt ttcagaactc ctgccttcat ggcatttttt tctctccaga
    12901 actgataccc tgcaatagct ttatattttt ttcagattat aaaccgacac cgcaaatata
    12961 tatataaggc aaactatcag caccgacatt cccagcacta tattgcttat atcgcttatc
    13021 cagcctataa ggaaaggtat cggggcacca cctgacacag ccatgatcat aagtccggat
    13081 atctcgctgc tgcgttccgg aaacctgcca accgtaaggg aaaatatcaa tggccagatg
    13141 ttggctactg ccagacctgt gagaaagaca agtccccatg cagctacttt tgaaggaata
    13201 acaagaagaa ccgccacaga agctattgaa agtatagtcg tccacatcag aaacctgggt
    13261 gcctgtatcc ttgttagcat gattgcccca aggaatgtcc cgagcatacg gccgaagaaa
    13321 taaacacttc gtcccgactc tgctgccgtc tgctcaattc cgaaatgttt catcagaaac
    13381 tgcccggaat tcgagttgaa tccaacatct acccccacaa caagaaatat agctacaacc
    13441 atcatcagta tgtaactgtt tccaagcagg ccaaatgaag ttttcaatgt tgcccggtaa
    13501 ccttcttcct tccgctcttc tatttttaca gaccccagcc atattacagc cagtaacgat
    13561 atgatgccaa aaacaagaaa ttcgagcttc caatcaccga attgggaagc aaagaccgcc
    13621 gccagcggag caccaatcat cgaaccaact gcctttacaa actgagagaa actcaaaaaa
    13681 ctcgatgccc tgtttgaagg tacaacatct aataacagcg ggtttgctga tacctgcaca
    13741 atggtgttac cgatgccaat aagagcaaac cctgccagca ccatcccgaa ggaataagaa
    13801 atgaaaggaa taagcagtcc ggctgctgtc aaaaccattc ctgcattaag cacaaacctt
    13861 ttaccatatc ttgcctgaat aattcctacc ggcaccgata gcaggaaaaa ccagagaaat
    13921 gttgccatcg gaattaactg ggcaagcgtc gcactgagat ccatatcgga acttaccctg
    13981 tcaacactta tccctaccag atccacaaaa ctcatcacaa aaaacgacat gagcactgga
    14041 agaatctttg aaaggctgat ttttgtattc ataatgagat ttttctgtta gacagtattc
    14101 ttttaacctt gaactttata cttttacatc aacggcagtg catgctgtac agccttccag
    14161 aaatgatcat caaagaggta agcactgcca agcagtgccg catcctcttt aagctcagaa
    14221 atacccacgt ctcctttcca gctttctttc ctcagcctcc cttcaaatac ctctccgaaa
    14281 agatgatatg cattcgaaat gttgcctcca ataaccagta tctcagcccc gaacttcttc
    14341 agccacggag ccagaaaaac tgcaaggtcg ttaccaaatt tcatgaataa cccctgaaca
    14401 gtcttgtccc cgtgtgccag atccgataat tccttcacac cacctgtttc tttccctgta
    14461 gcctccttgt aacttcttat aaaccatctg gttgaaaaat aatcatcagc aatactttca
    14521 ccataaggta aatggtatac acaacctatc ttaggaacat ccggaccatc aacaaccgga
    14581 atacggtcac taataaatgc cgaccccaat cctgtgccca gagtaataca catcgatttt
    14641 ctgtaatcct tagcttttcc tgcccacgct tcccctactg caaacgccga cgcatcgtta
    14701 ataaaacgaa taccgaaatc atccggcagt tcaagcttgc tcttcaccgc atctccaata
    14761 ttaaacccgt acaggttctc atatttcgaa acacccttta tataacatat cccattcacg
    14821 taatcaaacg gccccggcat tgcaaaacct attcctcgta tgtcatcaaa ttttaaatgc
    14881 cccattacct cctgcaaaca tgaactccaa acatcaatta tctcaaaagc ctgcgcctgg
    14941 ttatttaccg gcctgtcagc catagtttcc tttaaaactt ttcctgacgt catgtcaaca
    15001 gctgcagaag tgatatgact accgcctatg tcgacaccaa ttgcaatatt tttctccata
    15061 ataaattaac tgtttataac ccgatattaa taaactgcct gaaagtattt aaaattgata
    15121 aaaattcaaa atgaaactct gaacaaaatt gtctttcgtt cgaaaatcag aattaaaaag
    15181 atttctaatc aattctattc tattgaaaag caaaggctaa tttgacagca tggtaatccg
    15241 tcacagttgt ggtttctaca ggcttgagta attattttca acccttaatc actgccagag
    15301 gcgaaagacc agtaactaca tccaccagat cggcttgctg agccatcacc tcttcaatat
    15361 tcttgtatgc cgaaggagcc tcttcaaggt cattttttgt acgaattgaa tgcagtatat
    15421 cgagtttatc aagtcttcgc ttttcctcct caagattaag tgtacggata gcctggttgc
    15481 gtcccattac cctaccagca ccatgtgagc aactctcaaa actatccggg ttccccttgc
    15541 cctcaacaat ataactcctc gttccctgcg atccgggtat gataccttta agtccctttg
    15601 tagcctttgt ggcacccttc cggtgaataa ccagctcttc cccgaaatgt ttctcaatcg
    15661 ctgcgtaatt atgggctata tttattatct ctccatgaga aaattttgaa ccgaaataat
    15721 cgttgaacac accaattatc ctgtccatca tcagatagcg attacacatt gcaaattcta
    15781 tacagtactg catctccttc agataatcct gtccgatttt cgaatcgagc cgcaaccatg
    15841 caagttcagt ttttggtccg taatcttcac ctgcctccct gttaaggctg atggctttct
    15901 tgttgtaata gtcagccacc tgcttcccga tgtttctgct tcccgaatgt atcataatcc
    15961 atattgcacc atcatcacct ttctgaatct caataaaatg attgccacct ccaagtgtcc
    16021 ctacctgatg caatgcactg tcgtattccc tcgatactac aggaagctca aacggatcaa
    16081 aacccagttt ggtggg
//
LOCUS       DAOSNB010000022        15351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_3877_length_15351_cov_4.265560, whole genome shotgun sequence.
ACCESSION   DAOSNB010000022 DAOSNB010000000
VERSION     DAOSNB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15351
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_15351_cov_4.265560"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PLG42_01965"
     CDS             complement(<1..767)
                     /locus_tag="PLG42_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOU30007.1"
                     /translation="MKKTVFLPIFFLAFISCKQEKSDIIWSDEFNYTGLPDSSRWGYE
                     VGYIRNRELQYYTYRRQENAVVKNGNLMLIGRKEPWEGFEYTSASINTEGKHYFQYGL
                     IEARMKLPVGQGKWPAFWMMGKNRPQVGWPECGEIDIMEHINSTLILHGTAHWEDENR
                     NHKSSGGQIPCDVREYHVYAVDWTPDSLVWLVDGRRYFGISIKDSVNSTHEFHQPFYI
                     LLNFAIGGSWPGNPDSTSVFPDTVFVDWVRVHKFNRK"
     gene            complement(854..3049)
                     /locus_tag="PLG42_01970"
     CDS             complement(854..3049)
                     /locus_tag="PLG42_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOU30008.1"
                     /translation="MDKTVNHSLSVCISKFTVIIVLFSFSFSQARAQEILRWEPVAPG
                     IWKAEAGTPERYNLLTASGAKPVIKALEKMGSAPFPLDPALITAGIFDGQVYLKFPLV
                     RGERIFGFGLNFKTIYQRGKILTLHVDHYGGTDNGRTHAPVPFYVSDRGYGVFINSAR
                     YIRVWAGTSVNRDRQEPSAVRDRNTDKRWTSAPYSDAVDILVPAAGCEIYIFAGPSAL
                     DAIRRFNLFCGGGPLPPRWGLGFTQRVPTLYTSQQVIREAEEFDAHNFPLDFIGLEPG
                     WQSRAYPCTFEWDKTRYPDPEGFVRTMLEKGIRLNLWTNPYVSPESPVYEKILPYTGS
                     HNVWGGIVPDFSIKEAQEIFTGQLKKSQVSIGISGYKIDEVDGYDRWLWPDVAIFPSG
                     NSAEQMRQTYGLLLQRITSEMFREANRRTWGLTRASNGGGVSFPYVIYNDYYSHEDFI
                     TALINSGFCGVLWTPEVRSSKTAEEWIRRFQTVCFSPMAMINAWASGTKPWSFPEVED
                     DVRYYAYLRMQLIPYFYTEFAKYHYDGTPPFRSMVLEEGFNPVETEEIKNANLEDNPY
                     LEALRGEIKDQYMAGEYLLVAPLFAGQTSRKVILPKGNWYDFYTGEYAGNGEIITVTP
                     GLNRIPVFVKDGGIIPMMPPYSKAPAAGKKYDLEIRHYGTKEGHYLLYDDDGETFDYE
                     KGIFSWREIRVEPGKNGRMTGSISKAEKWKPDNIGNVTFRFMSLNQQKK"
     gene            3359..4306
                     /locus_tag="PLG42_01975"
     CDS             3359..4306
                     /locus_tag="PLG42_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HOU30009.1"
                     /translation="MKKILLPVLSFVFILPVIAQQSVNLRLNPEKNKTYRLRSVSHQT
                     VLQTVSGNQQTVESDVIYSFSLKMLDATPEFMVAEVHFDTLITKTNTMGRVETISSAD
                     EGDIKSSEASKVMSYIMNKLSKNAVFVKLNYAGQPLEIINAKILSDLILKDTASITLS
                     EPAASALKTQIINTVSENNLKTMIKNFTWHLPAAQVSKGTEWKIYDQINSGGMLLMVS
                     SKFRLEGLNGNNALITVESEIKVPENAPPIKSAGATVTYNSLTGMGKSNMVVDTETGL
                     LVENKTQTRITGNLSISAPGFSMEMPMDINGETVVRAVK"
     gene            4319..6697
                     /locus_tag="PLG42_01980"
     CDS             4319..6697
                     /locus_tag="PLG42_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU30010.1"
                     /translation="MKKSFIAFIVVLIFISFATEAQTLKGYVCYEKDKTPVQFATVAL
                     LQLPDSAIVTGVITLTDGAYTLENIKPGSYFVRASFVGYRTAGKLVNVAGTEKEIVVD
                     TIYLAETTAVLDEIMVVGERLKGKEMVDRTVYSIPEAVSKSSTNGYDLLKKIPQVNVD
                     FQNNITLNGSSNFIIQVDGRQRDREYLARLLPSDIQSIEIISNPGGKYEGNIDGVINI
                     ILKKEARFGMNGNLLEALKPFRKVTSVTQGSIDYSIGKITFYATGSYITQALNIFPKA
                     ESHFATADSSTYTGGGGNIKVSVGSLNAGMDYYMNDKNNFNFNISYKPISQNVHFPNT
                     ITLFKSDIPLNTIASVTDNNLKSDETAFSFFYKKTFKKPVEEFTTEVTWYMFRSVSGN
                     SFSNVRYPYGSEVVLDTYSRVEDDLNNRRYISTKLDYVYPLGMNAKLEAGYHFYFQKM
                     EYDFKINNQPESNLFEYSELRNSGYAGFALNLKKFGFQASLRIENSHIAADSVTKPDY
                     SCFLPSANIQYKFSASHNLKFTYNRRINRPGIYSMNPYYKVGQSYDITQGNPNLRPDY
                     RDRLQLTYTWNFGSNYFSPFVYYEYFSDRVSNRYTITRSPINGSLTTISKPFNLLNGY
                     ETGGGVNAVLWYVNISARIYNGHYNRYSEGSFTIPSKDYFSYTITSTAYKTLDKKKTI
                     TAFAYLQYNGVNIEAQTRTSTLPLYGFGGQWQLKSHSIGLFWLLPFSKITEYQTIETT
                     TPVYTSINKTGIDFGSWFQFQYMYRFNKGRNVKKLGRKVEIESDSKAHAIGQ"
     gene            complement(6705..7763)
                     /locus_tag="PLG42_01985"
     CDS             complement(6705..7763)
                     /locus_tag="PLG42_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30011.1"
                     /translation="MKKLFLFSFLVLFFYALSAQEEVTINITRKKIIFNSGMKLPVYV
                     NGTEVARLRNGETFSYKASLDALQPISVMVKSGLSKREIVFVVDASKVCNIETGFPGG
                     MLDLTLVSGGKIVPGTGMTARTKVNQKDLSVSYTMTQTLASDTIRLQWLEKGGTIMGM
                     SYIGGLTYLSLNKPAFSMTGFGGQVMITASYLKFKVPENKPGIRSWSSAVLGYTLMDQ
                     FSSSTTKLKNIDPGLPIETEFSSTSMNVMLSLNGGYTIGLGKFKNETKWKGAALELTY
                     RPSIVMTIPQEGETNVSFNATGFGFDINFNSFTSNAAKLAPKAQSKITLFILPPVKDM
                     PLYINVGYGLTFYRKIGR"
     gene            complement(7868..8815)
                     /locus_tag="PLG42_01990"
     CDS             complement(7868..8815)
                     /locus_tag="PLG42_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOU30012.1"
                     /translation="MKYLEKNIKSLLLGVATGDALGVPVEYNSRESLKEAPVTDMRGY
                     GSYDQPPGTWSDDTSLTLCLAEALTQGFDLKIIAENFVKWCFAGYWSANGSVFDIGGT
                     TYEAIYRLKKGTEPHLAGSDDKYSNGNGSLMRISPLVFYLLDKPADERFEKTRLVSSI
                     THRHIRSIIACFYYLEFARGILEGENVFDIYRNLQTEIPGFLASKGIDDHEISIFDRL
                     LKGIIYELPEEEIHSGGYVIDTLEASVWCLLTTDNYSDAVLKAVNLGQDTDTTGAVTG
                     GLAGLLYGIDNIPKKWLKKIARRKDIEDLAERLAKKLSE"
     gene            9059..10390
                     /locus_tag="PLG42_01995"
     CDS             9059..10390
                     /locus_tag="PLG42_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30013.1"
                     /translation="MTSRREFIQKAALGSAAIAFSAKSYSRILGANDRVRVGVVGFSD
                     RFRSSLFPAFMANAKDLNFEFTALSDIWRLRREEADKFFKSNGITLKLYRNNEELYSA
                     KDTDAVIISTADFQHALHCAEAVRAGRDAYVEKPFAETMADAREALKAVRETGKIVQI
                     GSQRRSAPNYIAANEYIRSGKFGKIVMVEMTWNVNQPGRWRRPNVVPLLKEEDTDWKR
                     YLMNRPFEPFNARKYLEFRLFWPYSSGIPGQWMAHQIDTVHWFTGLSHPRSVAANGGI
                     YLWKDGRTNFDTMTAVMDYGSDTDPNLSFLVTYSSRFTNSAGGTKELYFSNGGTLNLD
                     TNKVTPEGGLRANDAKAMNMQPNLLEPFDLPSFTVETSANTGGDPMTTAHMRNWMECV
                     RDRKKPNADVVAGYNHSIACIMVTAALRTGEKVTFDEKTQEVMAGGKVFQY"
     gene            10425..11450
                     /locus_tag="PLG42_02000"
     CDS             10425..11450
                     /locus_tag="PLG42_02000"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOU30014.1"
                     /translation="MRYLLITFLVLVNMAAYSQKVKLVHDEQKRKVDVFIGDKLFTSY
                     CYPANLEKPFLFPVYAPNGAVVTRGFPLEPRKGERVDHPHHIGLWFNHGNVNGLDFWN
                     NSSAIPVNRKDQYGHIVVKKIVKAKGGKTGLLEVVSEWKDNKENTVLTENTQYIFRAS
                     KTGWMVDHIATLTATNGPVTITDNKEGMFAIRVDRALEMPTNEPLIFTDEKGNPTTVK
                     VMDNTGVTGMYVSSNGNRGDAVWGTRNKWVILSGKKDNVPVSLAIFDHPKNPGFPAYS
                     HARGYGLFSVNNLGQKSYDPKQEEVVYKLAPGESMVLRHRFYIQSGSELTPEAAEAIF
                     NDFSKSY"
     gene            11609..12397
                     /locus_tag="PLG42_02005"
     CDS             11609..12397
                     /locus_tag="PLG42_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOU30015.1"
                     /translation="MVKNYLTLFFGAVFFFAAFMASGQAPRNREYYEIRIYHLKDKSQ
                     EERTDKFLKEAFLPALHRAGINKVGVFKPVESDTAFGKRIYIFIPFNNAIRMIELHSV
                     LENDRKYNQDGKDFLDAPFDNPPFTRYESIYLYAFKDMPAFRPPKFNTPPSARIYELR
                     SYESATEAKALKKIEMFNEGGEMKLFEKLGFNAVFFGQVLAGSTKPNLMYMTSFADQK
                     SREDHWNEFRNHPEWKTMSGLEQYKNTVSKANVFLLHPTDYSDF"
     gene            complement(12552..13919)
                     /locus_tag="PLG42_02010"
     CDS             complement(12552..13919)
                     /locus_tag="PLG42_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOU30016.1"
                     /translation="MKTSKKIILTEREMPRQWYNILADMPNKPLPPLHPATRQPLSPE
                     DLAAVFPMELIKQEVSTERYIDIPDEVLDLYKTYRPSPLYRAINLERKLGTKSKIYYK
                     YEGNNYSGSHKANTAIAQAYYNKKEGIRRITTETGAGQWGSAMSFACHHFGLELLVFM
                     VKVSYYQKPGRKVLMSAYGARVVPSPSTMTEAGRKVLKQDPESPGSLGIAISEAMEIA
                     LQDPYTKYSLGSVLNHVILHQTIIGQEALLQMEKAGDYPDVVIGCFGGGSNFSGIAFP
                     FLRERLLNGKNVRLLTVEPASCPKLTKGKFMYDFGDSAGMTPLLPMYTLGHNFIPEKI
                     HAGGLRYHGAGVLVSQLLKDGYIEARAKMQRECFEAGVLFASTEGILPAPESNYAIAA
                     ALDEARRADLEGVSKVILFNLSGHGHMDISAYEKYFEGNLPDHELTQSEVEAILSQYD
                     LPEAK"
     gene            complement(14152..15309)
                     /locus_tag="PLG42_02015"
     CDS             complement(14152..15309)
                     /locus_tag="PLG42_02015"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOU30017.1"
                     /translation="MVLQMDITPLNRWISDGGKPFIISGPCSAESEEQVIETARKLSE
                     IKQVKAFRAGIWKPRTRPGTFEGAGNVALKWLRRVKEETGLLTATEVANEKHVYEALK
                     YGIDILWIGARTSVNPFTVQEIADALTGVDVIVLVKNPINPDIDLWTGAIERVARASI
                     KKIGAIHRGFSAFEKTVYRNQPNWQIPIELRRRIPGLPILCDPSHIGGARKYIFEISQ
                     KAMDLNFDGLIIESHIDPDKALSDAAQQITPDTLKEILCRLVLRSPDPSDPRLLDELG
                     ELRQQIDIFDDHLLDLLERRMQIADKIGFFKKRNNITVLQSARWDEVISKAISKGKAK
                     GLSAELIDTIFKAIHQESINRQIRIVNNGLESNNIEERKETSDFTDFHNKT"
BASE COUNT         4388 a         3304 c         3215 g         4444 t
ORIGIN      
        1 tatttccggt taaacttgtg cacccttacc cagtcaacaa atactgtgtc agggaagacc
       61 gatgttgaat cgggattgcc gggccatgag cctccgattg caaagttcag caggatgtaa
      121 aagggctggt gaaactcatg tgtgctgttt acactgtctt ttatgcttat cccgaaatat
      181 ctccgtccgt caacaagcca taccagcgaa tcaggcgtcc agtcaactgc ataaacatgg
      241 tattccctaa cgtcgcatgg aatctgccct cctgagcttt tatggtttct gttttcatct
      301 tcccagtgtg ctgttccgtg gaggataaga gtactgttga tatgttccat aatatcaatc
      361 tctccgcatt cgggccatcc cacctgcggc ctgtttttcc ccatcatcca gaaggcaggc
      421 cactttccct gccccacagg cagcttcatc cttgcctcaa tgagaccgta ctggaaataa
      481 tgcttccctt cagtgttgat acttgccgaa gtatattcaa aaccttccca cggctctttc
      541 cttcctatta gcatcagatt accattttta actacggcgt tctcctgtcg cctgtaggtg
      601 taatactgaa gctccctgtt tcttatgtag ccaacttcgt atccccatct ggatgaatca
      661 ggaagacctg tataattgaa ttcatccgac cagatgatat cactcttttc ctgtttgcag
      721 gaaataaagg ccagaaagaa aattggcaga aaaactgtct ttttcattgt tgatggattt
      781 aatcattccg tttattgtct aaaaataaaa aaaattacct gacagatttg attatgaaat
      841 tatttaagga aaattatttt ttctgctgat tcaggctcat aaacctgaag gtcacgttcc
      901 cgatgttgtc gggcttccat ttttctgctt ttgatattga accggtcatc cttccgtttt
      961 tgcccggttc aaccctgatt tctctccatg agaaaatacc tttttcataa tcaaatgtct
     1021 caccatcgtc gtcataaagc aggtagtgtc cctcttttgt tccgtaatgc cgtatttcga
     1081 gatcatattt cttacctgca gccggagcct ttgaatatgg tggcatcata gggatgatac
     1141 cgccatcttt tacgaagaca ggtattcggt tcagtccggg agtgacagtt attatttcgc
     1201 cgttacccgc atattctccg gtataaaaat cgtaccagtt gcctttcggc agaataactt
     1261 ttcttgatgt ttgtccggca aacaggggag ccaccagaag atattctcca gccatatact
     1321 ggtcttttat ttctcctctt aatgcttcga gatatggatt atcttccagg ttggcatttt
     1381 ttatttcctc tgtctccacc gggttaaaac cttcctcgag taccatgctt ctgaatggtg
     1441 gcgttccatc gtaatggtat ttcgcaaatt cggtatagaa gtatggtata agttgcatcc
     1501 gcaggtatgc gtaatatcta acatcatctt caacttccgg gaacgaccag ggttttgtac
     1561 ctgaagccca tgcattgatc atagccatag gagagaagca gacagtctgg aatcttctga
     1621 tccactcttc agctgttttc gagcttctca cctcaggtgt ccataaaacg ccacagaagc
     1681 cactgttgat aagagcagta ataaaatctt catggctgta ataatcgttg tagatcacat
     1741 aaggaaacga taccccgccg ccgtttgatg cccttgtcaa tccccatgtg cggcggtttg
     1801 cttcgcggaa catttccgat gttattctct gaagcagcaa accatatgtt tgtctcatct
     1861 gttcggcgct gttacccgaa ggaaatatgg ctacatcagg ccataaccac ctgtcgtaac
     1921 cgtcaacttc gtcaatcttg tatcctgata tgccaattga gacctgactc ttcttcagct
     1981 ggccggtgaa tatctcctgt gcctctttta tactgaaatc aggaacaata ccaccccaaa
     2041 cattatgaga gcctgtgtag ggtaatattt tttcataaac aggggattcg ggtgatacat
     2101 aaggatttgt ccagaggtta agcctgatac ctttttcaag catcgttctg acgaaacctt
     2161 ccgggtcggg gtatcttgtt ttgtcccatt caaaagtgca agggtaggct ctgctttgcc
     2221 atcccggttc cagaccaata aaatctaacg gaaagttatg agcatcaaac tcttctgctt
     2281 cccttataac ctgttgtgaa gtataaagtg ttggtactct ctgggtgaac ccgagacccc
     2341 atcggggtgg caagggccca cccccgcaga agaggttgaa acgccttatt gcatcaaggg
     2401 ctgacggccc tgcaaaaata tatatctcgc atcctgcagc aggaacaaga atgtcaacag
     2461 catctgaata aggagctgat gtccaccgtt tgtcggtgtt acggtcacgt actgccgagg
     2521 gctcctgccg gtcgcgatta accgacgtgc cagcccagac ccttatatac cttgcactgt
     2581 tgataaatac tccgtatccc ctgtcagaaa cataaaacgg aacaggtgca tgagtgcgac
     2641 cattgtcggt accaccataa tggtcaacat gaagagtgag aatctttccg cgttgataaa
     2701 tggttttgaa attcagacca aaaccaaata ttctctcccc tcttacaaga gggaatttca
     2761 gataaacctg cccgtcaaaa attccggctg taatcagtgc aggatcgagg ggaaaaggag
     2821 cgctgcccat tttttcgagc gcctttataa caggttttgc ccctgaagcg gtaaggagat
     2881 tgtacctttc aggtgtgcca gcctccgctt tccatatacc cggcgctaca ggttcccatc
     2941 tcagtatttc ctgagctctg gcctgtgaaa aggaaaaaga aaatagaacg attattacgg
     3001 taaattttga aatacaaact gataatgaat ggttaacagt tttatccata agaaatatga
     3061 tttaaaatat tcctgctgta agataactaa aatgatttcc cattatgctt tttacgaaaa
     3121 aactcagatt taatcctata taacatgcag cgaaaaaata tttcaacagt ctattaaaca
     3181 tgaaaatttt tgccgaaaaa cctggctgga aagattttca tgaggataag attctggaag
     3241 gagctccgta cataatcttt aaaccttgtt ttaccggtag aaaataacaa attaccgata
     3301 acatattgga gacattaata ttatggtata gctttgaaaa attttaaacg tcttgacaat
     3361 gaaaaagata ttgttgccag ttctttcctt cgtttttatt ttgccggtta tagcgcagca
     3421 gagtgttaat ctccggttga atcctgagaa gaataagacc taccggttga ggtctgtttc
     3481 gcaccagaca gttttacaaa cagttagcgg taatcaacaa actgttgagt ctgatgtgat
     3541 atatagtttt tctctgaaga tgcttgatgc aacacccgaa tttatggttg ctgaagttca
     3601 ttttgacaca ttgattacaa aaacaaatac catggggaga gttgaaacaa tcagctctgc
     3661 tgatgagggc gatattaaat cgtcagaggc ttcgaaagtt atgtcgtata ttatgaacaa
     3721 actcagtaaa aatgccgtat tcgtaaaact gaattacgcc ggacagcctc ttgaaattat
     3781 caatgcaaag attctttcgg atttaattct gaaagatact gcttcgataa cgctttctga
     3841 gcctgcagcc tccgcactga aaacgcagat aataaatact gtaagcgaga ataatcttaa
     3901 aacgatgatc aaaaacttta cgtggcatct gccagcggca caggtttcaa aaggaactga
     3961 atggaaaata tacgaccaga ttaattcagg aggaatgctg ctgatggtaa gttcgaaatt
     4021 tcgactcgaa ggtttaaacg gaaataatgc tcttattacg gtagaatcgg agataaaggt
     4081 acctgaaaat gcacctccaa taaagtcagc aggagccaca gtaacatata acagccttac
     4141 cggtatgggt aaatcaaata tggtggttga tactgagaca gggctattgg ttgagaataa
     4201 aacccagacg cgcattacag gaaacctcag cataagcgcc ccggggttca gtatggagat
     4261 gcctatggat attaacggag aaaccgttgt aagagcagtt aagtaactaa ttttaaacat
     4321 gaaaaaatca tttatcgctt ttattgtagt actgatattt atatcttttg ccactgaagc
     4381 acaaactctc aaagggtacg tttgctatga aaaggataaa acaccggtac agtttgctac
     4441 tgttgccctt ttacaattac cggattctgc aatagttaca ggtgtaatta cccttaccga
     4501 tggagcatac acacttgaaa atataaaacc aggcagctac ttcgttaggg cgagttttgt
     4561 tgggtacagg acggcgggta agttagttaa tgtagcaggt actgagaagg aaatagttgt
     4621 tgacaccatt taccttgccg agactacggc agttcttgat gaaataatgg tagttggaga
     4681 acgtctgaag ggaaaagaaa tggttgaccg cactgtttat tcaattccgg aagcggtaag
     4741 caaatcttca actaatggat atgatcttct gaaaaaaatt cctcaggtaa atgtagattt
     4801 ccagaataat attaccctta atggaagcag taattttatt attcaggttg atgggaggca
     4861 gcgcgacagg gagtaccttg ccaggctact tccttccgat attcagagta ttgaaatcat
     4921 tagcaatccg ggagggaaat acgaaggtaa cattgacggt gttataaaca tcatcctgaa
     4981 aaaagaagca cgttttggaa tgaatggcaa cctacttgaa gccctgaaac cattccgtaa
     5041 agttaccagc gtcactcagg gaagtattga ttattcgata ggcaaaatta ccttttatgc
     5101 tactggctca tatataacgc aggcacttaa tatctttccg aaagccgaga gccactttgc
     5161 tacagctgac tcatcaacat acaccggtgg tggcggaaat attaaggtat cagtcggctc
     5221 tctgaatgct ggtatggatt actatatgaa cgataaaaac aattttaatt ttaatattag
     5281 ctataaacct ataagccaga atgttcattt tccgaatacc ataacacttt tcaagagcga
     5341 tattccgctg aacacaattg catcggttac ggataacaat ttaaaaagtg acgaaacagc
     5401 attttcattt ttctataaaa agacattcaa aaaaccggta gaagagttta ccactgaggt
     5461 gacatggtat atgttcaggt ctgtttcagg taattcgttt tctaatgttc gctatcctta
     5521 cggaagcgag gttgtgcttg atacatactc gagggttgag gacgatttaa acaaccggag
     5581 atacatatca acaaagctcg attatgttta tcctttgggt atgaacgcta aactcgaggc
     5641 tggctaccat ttttactttc agaaaatgga atatgatttt aaaataaaca atcagccaga
     5701 aagcaacctg tttgaatatt cggagttaag aaattccggt tatgccggat ttgccctgaa
     5761 tcttaaaaaa tttgggttcc aggcatcgct gaggattgaa aacagccata tagcagctga
     5821 ttcggttaca aagccagatt attcatgttt tctgccatcg gcaaatattc agtataaatt
     5881 ttcagcatct cacaatctga agtttactta caaccgcaga atcaatcgtc ccggtattta
     5941 cagtatgaac ccgtattaca aagtggggca gtcgtacgat attactcagg gtaatccgaa
     6001 tcttcggcct gactatcgcg accgccttca actgacatac acctggaatt tcggaagcaa
     6061 ctatttttca ccttttgttt actatgaata tttttctgac agggtaagca accggtacac
     6121 aataacccgc tcacctataa acggttctct tactaccata tcaaaacctt ttaacctcct
     6181 taacggttat gaaaccggtg ggggcgtaaa tgcagtttta tggtatgtga acatcagtgc
     6241 caggatttac aacggccact ataacaggta ttctgaaggt tcattcacaa taccatcaaa
     6301 agattatttt tcctatacta ttacgtcaac agcatacaaa actcttgaca aaaagaagac
     6361 tataactgca tttgcttacc tgcagtataa tggtgtgaat attgaagcac aaacaaggac
     6421 aagcaccctt cccctgtatg gttttggtgg tcaatggcag cttaaatctc attcaatcgg
     6481 gcttttctgg ctgttgcctt tctcgaagat aacagaatat cagactattg aaactacaac
     6541 acctgtttat acctcaataa acaaaaccgg aatagatttc ggaagctggt tccagtttca
     6601 gtacatgtac aggttcaata aaggcagaaa tgtaaagaaa ctcggccgta aagtagaaat
     6661 cgaaagtgac agcaaggccc acgcaatagg gcaataggat tcctttacct tccgattttt
     6721 ctgtaaaagg tcaaaccgta gccaacgttg atatagagtg gcatatcttt tacaggtggc
     6781 agaatgaata atgtaatttt tgattgtgcc tttggagcca gctttgcagc gtttgaggtg
     6841 aaactgttga agttgatgtc aaaaccaaaa ccggttgcgt taaacgaaac atttgtctct
     6901 ccctcctgtg gtattgtcat tacgatgctt ggcctgtatg taagttcgag tgctgcacct
     6961 ttccattttg tttcattctt gaatttcccc agccctattg tgtaacctcc gttaagtgaa
     7021 agcattacgt tcatgcttgt tgaagaaaat tctgtttcga taggcaaccc tgggtcaata
     7081 tttttaagtt tggttgttga actggagaac tgatccatca atgtgtaacc caatactgca
     7141 ctgctccatg aacggatacc gggtttgttt tcaggaacct taaatttgag gtaagaagct
     7201 gtaatcatta cctgtcctcc gaatcctgtc atagagaacg caggtttatt aagactcaga
     7261 taagtgagac caccgatgta cgacataccc ataattgttc ctcctttctc aagccactgg
     7321 agcctgattg tgtcagatgc aagtgtctgt gtcattgtgt acgatactga aagatccttc
     7381 tggttgactt ttgttctggc agtcatacct gttcccggta ctattttgcc acccgataca
     7441 agggtgaggt ccagcatccc acccggaaaa ccggtctcta tgttacatac cttgctggcg
     7501 tcgactacaa aaacaatctc tcttttgctt aaaccgcttt ttaccataac tgaaatgggc
     7561 tgtaatgcat caagagatgc tttgtagcta aatgtttcgc cattacgcaa ccgggccact
     7621 tcagtcccat taacgtaaac cggtaatttc atgcctgaat tgaaaatgat ttttttccgt
     7681 gtaatattga tagtaacttc ttcctgtgcc gaaagggcat aaaagaagag aaccagaaaa
     7741 gaaaaaagaa atagtttttt catgattttg tcaatcaaga taataggtac tttgttgctt
     7801 atgttaatat aacaaataac gttatgcgat aatttttatt tattttatta ttttatgatt
     7861 aaacatttta ttctgaaagt ttttttgcaa gcctctctgc aaggtcttcg atatcctttc
     7921 gtcgtgctat ctttttaagc cattttttag gaatgttgtc aattccgtat aaaagccctg
     7981 caagtccgcc tgttacagct ccggtggtat cggtgtcctg ccccaggtta accgctttca
     8041 gtaccgcatc actgtaattg tctgtagtaa gaagacacca cacagatgct tcgagtgtgt
     8101 ctatgacata accaccgcta tggatttcct cttcaggcag ctcataaata attcctttaa
     8161 gcaggcgatc gaatattgag atttcgtgat catcaatccc cttcgaggca aggaatccgg
     8221 gaatctcagt ctgaaggttc cggtagatgt caaagacgtt ttctccttcg agtatccctc
     8281 tggcaaattc aaggtagtag aaacatgcta ttatcgacct gatgtgacgg tgagtgattg
     8341 atgaaacgag ccgggttttt tcgaatcttt catcagcagg cttgtcaagc agatagaata
     8401 caagaggtga aattctcatc agtgaaccgt ttccgttgga atatttatca tcgctgccgg
     8461 caagatgtgg ttcagttccc ttttttaaac ggtatatggc ttcgtaggtt gttcctccta
     8521 tgtcgaaaac actgccgttg gcgctccagt acccggcaaa gcaccacttt acaaaattct
     8581 cggcaataat tttcaggtca aagccctgtg taagtgcctc tgcaaggcac aatgttaagg
     8641 aggtatcatc cgaccatgtc cctggaggct ggtcgtagga tccgtaacct ctcatgtccg
     8701 tcacaggagc ttctttaaga gactctctac tgttgtattc tacaggtaca ccaagcgcat
     8761 cacctgtggc aaccccaaga agcagggact tgatattttt ttcaagatat ttcatttctg
     8821 aaaatatgat aattagttaa acctgaaatg actgcgcaat aaaaaagcca gtaaaattaa
     8881 atcgttatta ttgttggcct aaaattaagc tttttcaaat ttaatgtcag ttaaaattga
     8941 agaaaaacat ttaagaatta atactgaaaa aaatcattgt taacgttaac gatatttcaa
     9001 ttgtttgatt atttttacat ttcagacaat gattattaac ctgtaaaata tttttgatat
     9061 gacaagtaga agagaattca ttcagaaagc cgcacttggt tcggctgcaa tagcattcag
     9121 tgcaaagagt tattcccgta ttcttggagc caatgacagg gtacgggttg gagttgtagg
     9181 tttttcagat cgtttcaggt catcactctt tcccgccttt atggctaatg ccaaagatct
     9241 gaatttcgag tttactgcac tctccgatat atggagactg cgccgtgagg aggcagacaa
     9301 gttttttaaa tctaatggaa tcactctcaa gctctacagg aataatgaag agctttacag
     9361 tgcgaaggac accgatgcag tcataatcag cactgccgac tttcagcatg cgcttcattg
     9421 tgctgaagca gtgagggcgg ggcgtgacgc gtacgtcgaa aaaccttttg ctgagacaat
     9481 ggccgacgca cgtgaggcac tgaaagctgt cagggaaacc ggaaaaatcg tgcagatagg
     9541 atcacagcgc cggagtgcac cgaactacat agctgcaaat gagtatatcc gctcgggcaa
     9601 gttcggtaaa atagtgatgg tcgaaatgac atggaatgtt aaccagcctg gccgctggag
     9661 aagacctaat gtggtacctt tacttaaaga ggaagatacc gactggaaac gttacctcat
     9721 gaacagaccc tttgaaccat ttaatgcaag aaaatacctt gagttccgtc tcttctggcc
     9781 atattcatcg ggtatacccg ggcagtggat ggcacaccag attgataccg tgcactggtt
     9841 cacaggcctt tcacatccga gaagcgtcgc agccaacggc ggcatatacc tgtggaaaga
     9901 tggacgtacc aacttcgata ccatgacagc agtgatggat tatggttcag ataccgaccc
     9961 gaatctcagc ttccttgtga catattcatc acgcttcacc aattcggcag ggggaacaaa
    10021 agagctctat ttctcaaacg gcggaacact caacctcgat accaataagg tgacaccaga
    10081 gggcgggcta agagcaaatg atgcaaaagc aatgaacatg caacccaacc tgctcgaacc
    10141 attcgacctt ccctcattta ccgttgagac atctgcaaat acaggcggcg acccgatgac
    10201 aactgcccat atgcgcaact ggatggaatg cgtccgcgac cgcaaaaaac ccaacgccga
    10261 cgttgtggca ggatacaacc attcaatcgc ctgcattatg gttactgcag cactccgaac
    10321 aggcgagaaa gtcactttcg acgaaaaaac tcaggaagta atggccggag gtaaagtgtt
    10381 tcaatattaa tatattacaa atcaattatt taaaattatt gaaaatgaga tatttactga
    10441 taactttcct ggtacttgtt aatatggctg cttacagcca gaaagtgaaa ctggttcacg
    10501 acgaacagaa aagaaaggtg gatgtattta ttggcgataa actctttacc tcgtactgct
    10561 atcctgccaa tctcgaaaaa ccttttctgt ttccggtata tgcccctaac ggtgcggttg
    10621 tgacaagggg ttttccacta gaaccgagga aaggagaacg tgtcgaccat ccacaccaca
    10681 ttggcctttg gtttaaccac ggaaatgtga acggactcga cttctggaac aactcttcag
    10741 ccataccggt aaaccgtaaa gaccaatacg gccatatcgt ggtaaaaaag atagttaaag
    10801 ctaaaggcgg taaaacaggt ctgcttgagg ttgtttcgga atggaaagac aataaggaaa
    10861 acactgttct tacagaaaac acacagtata tcttcagagc ctcaaaaact ggctggatgg
    10921 ttgaccatat tgcaaccctt acagcaacaa acggcccggt tactataacc gacaataagg
    10981 agggaatgtt tgccatccgc gtcgaccggg cgttagagat gccaacaaat gaaccgctta
    11041 tttttactga cgaaaaaggc aaccccacaa ccgtaaaggt tatggacaat acaggcgtta
    11101 caggaatgta tgtttcgagc aacggtaaca ggggtgatgc tgtttgggga acacgcaaca
    11161 agtgggtgat tctcagcggc aaaaaggata acgtgccggt atcactcgcg atatttgacc
    11221 atccaaaaaa cccgggcttc ccggcatatt cccatgcccg tggatacgga ctcttttcag
    11281 taaataacct cggacagaaa tcatatgacc ccaaacagga agaagtggtt tataaactcg
    11341 cccccggtga gtcaatggta ctaaggcata gattttatat tcagtcgggc tccgaactaa
    11401 ccccggaagc agccgaagca atattcaacg acttctcaaa atcttactga ttgtattgaa
    11461 taataatcct caaaaaatcc tttccccctc tccatttgga gagggggacc aagggggaga
    11521 ggtcgataac ccatctggag aggggaagag gcgtaaatat ctgaattaat cagtattttt
    11581 atatcaataa aaattaaaaa ttgaaaaaat ggtaaaaaat tatcttacac tattttttgg
    11641 agctgttttt ttctttgcag cttttatggc ttcaggtcag gcaccccgga acagggaata
    11701 ttatgaaata agaatttatc atctgaagga caaatcacag gaggaaagga ccgataaatt
    11761 tcttaaggaa gcctttttac ctgcacttca tcgcgcaggc attaataaag ttggtgtttt
    11821 taaaccagtg gagtctgata ctgcattcgg caaaaggatt tatattttca tacctttcaa
    11881 caatgctatc cgtatgatag aattacattc tgttctggaa aatgacagaa aatataatca
    11941 ggatggaaaa gatttcctcg atgcaccttt cgataatcca ccctttacca ggtatgaaag
    12001 catatatctt tatgcattta aagatatgcc ggcattcaga ccaccaaagt tcaatactcc
    12061 tccatcagca aggatttatg aacttcgcag ctacgaaagt gcaactgaag caaaggcatt
    12121 gaagaagatc gaaatgttca atgagggagg ggagatgaag cttttcgaaa aactgggatt
    12181 taatgcggtc tttttcgggc aggttctggc aggcagcaca aagcccaacc ttatgtatat
    12241 gacatcattt gccgatcaga aatcgaggga agatcactgg aacgagttcc ggaatcatcc
    12301 tgaatggaaa accatgtcgg gtctggaaca atacaagaac accgtttcaa aagctaatgt
    12361 attcctcctc cacccaacag attattccga cttctaaaat aaaggggctg tctcaaaaga
    12421 tgtatatagg gtaagtttat aaaaataaaa tctaaaacat tgtatattaa taatttcctt
    12481 ttcaggaaat cagggtgaaa acaaaaaaat gacttttgag acagcccttt ttaaatgtgc
    12541 tttcagtact tttattttgc ttctggcagg tcatattgtg aaagtatcgc ttccacttct
    12601 gactgtgtta gctcatggtc gggcaggttg ccttcaaagt atttctcgta agctgaaata
    12661 tccatgtgtc cgtgaccgct gaggttgaac agtatcactt ttgatactcc ttcgaggtca
    12721 gctcttcgtg cttcgtcgag ggctgcagct attgcgtagt tcgattcagg ggcagggagg
    12781 ataccttcag tgcttgcaaa gagtactccg gcttcaaagc attctctctg catttttgcc
    12841 ctcgcttcga tgtaaccgtc cttcagcagc tggctcacca gcacgccggc tccgtgatag
    12901 cgcaatccac ctgcatgtat cttttcaggt atgaagttgt gcccgagagt gtacattggt
    12961 aaaagcggag tcatgccggc tgaatcgcca aaatcgtaca taaattttcc ttttgtaagt
    13021 ttcggacagg atgccggttc aacggtcagc aatcttacgt ttttaccatt gagcagccgt
    13081 tcgcgcagga acgggaatgc tatgcctgag aagtttgaac caccaccgaa acagcctatt
    13141 acaacatcag gataatcacc agctttttcc atctgaagca atgcctcctg accgatgatt
    13201 gtctgatgca ggatgacatg gtttaataca cttccgaggg aatattttgt ataaggatcc
    13261 tgcaatgcaa tctccattgc ttcggatatt gcaatgccaa gacttcccgg tgactcgggg
    13321 tcctgtttta gaacctttct tcctgcctca gtcatggtgc ttggcgaagg aacaaccctt
    13381 gcaccatatg cactcataag caccttgcgg cctggcttct ggtaataact caccttcacc
    13441 atgaacacca gcaactcgag tccgaaatgg tgacatgcaa aactcatggc actgccccac
    13501 tggcctgcac cagtttcagt tgtaattctc cttatgccct cttttttatt gtaatatgcc
    13561 tgtgctatag cagtattggc tttgtgcgag cctgaatagt tgttcccttc gtacttataa
    13621 taaattttgc tttttgtacc cagcttcctt tcaagattta tggctctgta aagcggggag
    13681 gggcggtagg ttttgtagag gtcaagaact tcatcaggta tgtcgatata cctttcagta
    13741 gaaacctcct gttttatgag ttccatgggg aatacggctg ccagatcctc cggactcagc
    13801 ggctggcggg ttgcaggatg cagcgggggc aaaggcttgt tgggcatatc agcaaggatg
    13861 ttataccatt gcctcggcat ctctctttct gtcagaataa tcttctttga tgttttcata
    13921 tttctgttat ttaagggtta ataattgagt cattaaaaca taaaaggcac gctgtgaaaa
    13981 gcgtgccttc aatagtttct ttattattta atgtcatgca acggcacttt tcacagcgat
    14041 ccggagttcg ctgcgccagc accagatgta taacgtaatt tgtttcatca cctcaaaact
    14101 atgattaatt tttaaaaaac caaaacaaat agttgatttt ttttaaaatt tttacgtttt
    14161 attgtgaaaa tctgtaaaat ccgaagtctc ttttctctct tcaatattat tgctttccag
    14221 tccgttattt acgattctta tctgcctgtt aatggattcc tggtggattg ccttgaatat
    14281 cgtgtcaata agttctgcac tcagtccttt tgcttttcct tttgagatag ctttgctgat
    14341 cacctcgtcc caccgggcgc tctggagaac tgttatgttg tttctctttt tgaaaaatcc
    14401 gattttatcg gctatctgca ttcttctctc gagcaggtca agaaggtgat cgtcgaatat
    14461 gtcgatctgc tggcggagtt cacccagttc atcgagcagt cgcgggtcgg aagggtcggg
    14521 tgagcgaaga acaagccggc agagtatctc cttaagggta tcgggtgtta tctgctgggc
    14581 tgcatcactc agagctttat cagggtcgat gtgggattct attatcagac catcaaagtt
    14641 aaggtccatt gccttctgcg agatctcgaa gatatacttt ctggcacctc caatatggct
    14701 cgggtcgcac agtatgggca gcccgggtat tcttcttctc agttctatcg ggatctgcca
    14761 gttaggctgg ttgcggtaga cagttttttc gaaagctgaa aatccgcggt gtatggctcc
    14821 aattttctta atgcttgccc ttgcaaccct ctcaatggca cctgtccaga ggtcaatatc
    14881 aggattgata ggatttttta caaggactat tacatccaca ccggtcagag cgtcggcaat
    14941 ctcctgaacg gtaaaaggat tgaccgaggt gcgggcacct atccagagaa tgtctatacc
    15001 atatttaagt gcctcgtaga catgtttttc gttagccact tcagtggccg taagcaaacc
    15061 ggtctcttcc tttactcttc ttagccattt cagtgcaaca ttccctgccc cttcaaaggt
    15121 gcctggacgt gttctcggct tccatatacc agcacggaaa gcttttacct gctttatctc
    15181 tgatagctta cgcgctgtct ctattacctg ttcttcgctc tcagcactgc agggacctga
    15241 gatgataaac ggcttgccac catcactgat ccatctgttg agaggcgtta tgtccatctg
    15301 taataccata atcgtgctaa tttaaaattt tctgtatcct gttagcctca t
//
LOCUS       DAOSNB010000023        14853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4058_length_14853_cov_4.490201, whole genome shotgun sequence.
ACCESSION   DAOSNB010000023 DAOSNB010000000
VERSION     DAOSNB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14853
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4058_length_14853_cov_4.490201"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLG42_02020"
     CDS             <1..885
                     /locus_tag="PLG42_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU30018.1"
                     /translation="DFLEKPLDLDRLLITIRNATDKSRLVTQTKVLKNKVSKMYEIVG
                     QSPAILQVKEMIDRVAPTEARVLITGASGTGKELVAHQIHEKSNRAKGPFVEVNCAAI
                     PSELIESELFGHEKGSFTSAIRQRIGKFEQANGGTLFLDEIGDMSLSAQSKVLRALQE
                     NKITRVGSDKDIHVNVRVIAATNKNLLKEIEQNTFREDLYHRLSVILIHVPTLNERLE
                     DIPMLAEHFNTLICNEYGMSKKIITDDAIKELQKIQWRGNVREFRNVLERLIILCKDR
                     ITGKDVIAYAHPVSGLKL"
     gene            complement(955..2502)
                     /locus_tag="PLG42_02025"
     CDS             complement(955..2502)
                     /locus_tag="PLG42_02025"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU30019.1"
                     /translation="MSKLISIIAILFFPVSVFAQQINDTIIIKSDTADTERVARDLDS
                     LLHTWYVQTAFMNLPADTMEEVIPEFPDSVYSERLSKISTLVNLPYNNIIRNHIHVYT
                     DRKREVLRAVLGLADYYFPMIEDIFESYGLPVELKYMAVIESALNPNAVSRAGATGLW
                     QFLASTGRMYGLTINSLVDERRDPVKATHAAARYLKDMYNIFGDWVLVIAAYNCGPGN
                     VNKAIARSGNRKDYWEIYYRLPRETRGYIPQFVAATYAINYYKEHNISPLPVKIPMAT
                     DTIMVHKDIHLNQISEVMGISLDELRYMNPQYRTGLIPGNTKPQPLRLPLSALSGFIQ
                     MQDSIIHYKSDVYLNRATAKVYPVRSYYAPKSIAGKTKLYYTVKEGDNLGYIAEWYRV
                     RLSDLRYWNDLYTNTIRVGQKIAVFVDPSKVEFFSRINDMTFAEKQALRGTPVPVNQT
                     SALPASGSEITDGEYFIYTVRRGDTIWDIVKQFEGVTVTEVLKANDISNPSKIQAGQK
                     LKIPKKT"
     gene            complement(2505..3107)
                     /locus_tag="PLG42_02030"
     CDS             complement(2505..3107)
                     /locus_tag="PLG42_02030"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOU30020.1"
                     /translation="MLFAVLVSEQAAAQDSVQVITGKRRKFIPDPTKATMLAAAFPGL
                     GQIYNRKYWKVPFVYAGFGGVGYAVYYNTKWYNTYIKAYQDFTDEIPETDSYIKLIRN
                     IKPEEYDPVLHPSTYNLSTASWIKDQMMRQIDYFKKYRDLSYIGIAAWYLFTILDASV
                     DASLFDYDISESLALKIEPVKIPLYNFVGAGINVKLLVTF"
     gene            complement(3208..4089)
                     /locus_tag="PLG42_02035"
     CDS             complement(3208..4089)
                     /locus_tag="PLG42_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOU30021.1"
                     /translation="MTKKNALGRGLGALIEGIDKETLEKKVEPNLQIPVDLIDSNPFQ
                     PRTRFDEEALGELAASIRQLGIVQPLTVRETGTGRYQLIAGERRLRAARMAGLTHVPA
                     FVRTADDQAMLELALVENIQREDLDAIDIAISYSRLVEECKLTQEQLSERVGKQRSTV
                     ANYLRLLKLPPEIQLGIKNKQITMGHARTLINIEDPKKQIKVYYKIIDEDLSVRATEE
                     LVRTLQSEDLKDPVKAQKKKKLNEDFAQLSAHLERIFSSKVVFRINEKGKGKIVIQFN
                     SPEEMERILGLFDRLNS"
     gene            complement(4102..4893)
                     /locus_tag="PLG42_02040"
     CDS             complement(4102..4893)
                     /locus_tag="PLG42_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU30022.1"
                     /translation="MGRIIALANQKGGVGKTTTAINLAASLAALEKKVLVVDNDPQAN
                     ATSGIGVDVKKITVTIYDCLIDGKDPREAFMPCGIDNLMVIPSNIDLVGAEIEMLEMP
                     GREKVLKKVLEGIRNEFDYILIDCSPSLGLLTVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTIKIIQSNLNPSLEIEGFLLTMYDSRLRLSNQVVEEVNKHFQQMVFKTIIQRNVK
                     LSEAPSFGQPAILYDAESRGTVNYLNLAQELIDKHKDTVKQMQAN"
     gene            5227..6180
                     /locus_tag="PLG42_02045"
     CDS             5227..6180
                     /locus_tag="PLG42_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOU30023.1"
                     /translation="MNNTEVRSDWLVIVNPNAGSGKGGNDWERIAALLVRAGIHFSAR
                     FTGKRGDAIMISREGIEQGFRKIITVGGDGTLNEVLNGAYSQNSCTPSEVCLALIPVG
                     TGNDWGKMFGIPLIYEGAIKIIKEGKTLMHDVGVVSYRNGEETNTRYFINIAGVGFEA
                     LVVKKTNIQKEKGKSNRAIYLYNLLSSLIAYKHTEVTLYIEKQIIKNRIFSINVGNGR
                     YCGGGMRQTPDALPDDGLLDVTVIKEIGRIEIIKNLKILYDGTILSHPKVDGYRVKSF
                     RVESVPQLMIEADGELLGHTPAEFSVIPEGIRIVYGSRLIQ"
     gene            complement(6170..7309)
                     /locus_tag="PLG42_02050"
     CDS             complement(6170..7309)
                     /locus_tag="PLG42_02050"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOU30024.1"
                     /translation="MDKKIKMMKKCHNCIIYPLLILFVAWFFSCSGRSNKKEVENEPV
                     KELSEPEEMAAEMIKLVAPPENAELKLNEKIKIVLEPDARVLPDSVRIWFDGKDAGVI
                     RNNIWEYEIPSSLNSKTGKKAIKVVAYKRRYRSQTISRFVTILSDVAPRRNGYRVINV
                     YPHDREAFTQGLVYYGGYLYEGTGQETRSNLRKVNLVTGEVLNQHNLDSKLFGEGIAI
                     FDGKIYQLTWQSKVGFVYDLESFRMLNRFYYQTEGWGLTTMDDKLVMSDGSNILYIID
                     PAMFLPVAQLEVYDNKSMVKDLNELEYIKGEIWANIWMTDLIARIDPASGKVLAYIDL
                     KGIMNDPSFDTASNVLNGIAYDSENDRIFVTGKNWPKLFEIKVTE"
     gene            complement(7294..7746)
                     /locus_tag="PLG42_02055"
     CDS             complement(7294..7746)
                     /locus_tag="PLG42_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30025.1"
                     /translation="MDNGFDLLNIRIAVDFDGTIVENAYPSIGKEKLFAFSTLKELEK
                     RGAKLILWTFRTGKELEEAVEFCRQHGIEFYAVNKNYPEEVFNEQTVSRKIDVDIYID
                     DKNLGGFPGWSQVWQMLFPYEIQEEMAKRKILRAGNIFRRIFRKWTRK"
     gene            7876..8595
                     /gene="pssA"
                     /locus_tag="PLG42_02060"
     CDS             7876..8595
                     /gene="pssA"
                     /locus_tag="PLG42_02060"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOU30026.1"
                     /translation="MRHIPNMITSLNLASGFLSIIFIIKGNTAAACWLVLAAMVFDFL
                     DGFASRILNAYSDLGKELDSLADVVSFGVAPGIIIYSTGIFFSDNNVYSIIYAVISAF
                     YAVCAGLRLAKFNIDTSQSVVFRGLPTPAAALSVISLCLAAAYSDNFFVTLFTKYQFL
                     TIFFVIIVSLLMVSNLPMISLKIKNLKIKGNEARYIMAALSIIFVAVFGARGFALIIP
                     AYIILSLAWAPFLKNEISRHP"
     gene            complement(8722..9843)
                     /locus_tag="PLG42_02065"
     CDS             complement(8722..9843)
                     /locus_tag="PLG42_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOU30027.1"
                     /translation="MMSHAFLQHYWWIVISLLGGLLGFLMFVQGGQTFIYSLPANAMQ
                     RKMIVNALGRKWEFTFTTLVTFGGAFFASFPLFYATSFGGAYWLWMLILFSFVIQAVA
                     YEYRSKPSNVLGSNTYDIFLLLNGSLGPFLTGIAVATFFTGAHFSLDNLNRVTWQTNL
                     HGLEALADIRNIALGFALLFLARVNGLLFLLNTVDDEEIISKARNKVLFNALFFLVFF
                     LYFILTIFLKEGFAVEPATGEILVQKFKYLKNLLQMPPVLIVFIAGVAGVLYGIYITV
                     FAGSRKGIWFSGAGSVMAVFALFLTAGLNNTAFYPSVNDIQNSLTLQRASSSRFTLET
                     MMYVSFIIPFVIAYIWYAWRAVTQRKITAEDIEKDGHAY"
     gene            complement(9840..11411)
                     /locus_tag="PLG42_02070"
     CDS             complement(9840..11411)
                     /locus_tag="PLG42_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOU30028.1"
                     /translation="MIENIDLSLVSWSRAQFALTAIYHWFFVPLTLGLSFIIAMMETI
                     YYRTGSEEWKRITKFWMKLFGINFAIGVATGIILEFEFGTNWSAYSWFVGDIFGTPLA
                     IEGILAFFLESTFIAVMFFGWNRVSKRFHLVSTWLVAIGASLSALWILVANAWMQNPV
                     GMTFNPETARNEMTNFLEVLFNPVAADKFLHTITSGFVLASVFVAGISAWFLLKKRET
                     YLATRSILIAGIFGFLSSVLLAITGDMSARSVAKYQPVKFASFEALYEGKSRAPLVAA
                     GILKKGEKGVGRKNMPEFIFAIEIPNLLSFMTGGSKDAYVPGIYDHIEGNPRAGILSF
                     AEKKERGQKARETLLEYKKAKEAGDETKASDIAALFTSKDFRENYFRYFGYAYIDNPE
                     DIIPDIRVSFYTFHLMVLLGFFFILLFALMLWFLFRNNIRKHKWLLWIMLLSIPLPYL
                     ASELGWVLAEMGRQPWIIQDLMPVSVGVTNLKSGNVQTTFWLFAVLFTALLIAEISIM
                     VKQVKNGPEKTEEKL"
     gene            complement(11418..11639)
                     /locus_tag="PLG42_02075"
     CDS             complement(11418..11639)
                     /locus_tag="PLG42_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOU30029.1"
                     /translation="MSFLNIFRKIFVFYLDGFRNMSDWGRKVWIIILIKLFIIFIILR
                     LFFFPDVLKKSFPDDSNRSNYIREHLLSK"
     gene            complement(11661..12827)
                     /locus_tag="PLG42_02080"
     CDS             complement(11661..12827)
                     /locus_tag="PLG42_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOU30030.1"
                     /translation="MKNFIRNILILAGIVFVVFCVWYFREIVIYILVAGVLSIMGRPL
                     VDLFDRIKIRKISIPKAVSALLALLIIWGLIFLFFYIFVPLVASQIQQLSSIDSEQLV
                     KIIEEPIRKTENLLRALNPDLEEISVRAYIASKITDILSIEFVQDMIGSAARTMDNIV
                     IAFFSISFITFFFLKDQHLFFESIVMWVPDKYVDNVTRALYSIKALLTRYFVGIVIQS
                     TCIMIMMTTGMTVVGIDFHQALVMGLIAGILNVIPYVGPWLGFFITVTMGIASHLGYD
                     FQSVIVPLVWKMALVVAITQATDNVVFQPVIFSSSVRAHPLEIFVVVLAAGFAAGIPG
                     MILGIPAYTVLRVFAREFFYNLKAVQKITSSLPDTEEIMEKIKEEEEKEHGKNL"
     gene            13050..13469
                     /locus_tag="PLG42_02085"
     CDS             13050..13469
                     /locus_tag="PLG42_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOU30031.1"
                     /translation="MLTRRSDKPFLWSDLFDDDFFFPFFTTRYHSMPAVNIKEDEKKF
                     TLDLAVPGIDKKDLKIELNEDVLTISSERKEEKEESREDFKRREFSYASFCRSFSLPE
                     NVDREKIEASYKDGILTVTLPKMHEEKSKVTKQIKIS"
     gene            complement(13527..>14853)
                     /locus_tag="PLG42_02090"
     CDS             complement(13527..>14853)
                     /locus_tag="PLG42_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOU30032.1"
                     /translation="SGSTFTSTRQGKQKHNCTAMKSESKSKDRKPDDRIIEITPDVKW
                     IGVLDYDIVTFDIVMETEFGTTYNSYFINAEKKAVVEAAKDRFSDVQISKLKKLCDPS
                     EIQYIILDHTEPDHSGNTGRLLELAPDATVVGSGNMIRYLSDIINKPFKSIAVKDGDT
                     LSLGNKTLRFIAAPNLHWPDSIFTWLEEDRVLFTCDMFGAHYCSEEMFNDFSEDYFKS
                     FKYYFDVILRPFSRFALKAIEKIKPLDIRFICPGHGPVHHRDIERIITLTEELSREYV
                     EITSGGEVKNVLIAYVSAYGFTGKMAEIVAEGIKEIPGVKATLLDIENISAGELDKEL
                     MKADALLVGSPTINQNTLLPVYRLFAFINPLRDRGKPAGSFGSYGWSGEAPGIIAAAL
                     KGLKLAYFENPLTVKFFPGNEKAGLLKEYGRRFGEFVLSQCSGKNLNKE"
BASE COUNT         4366 a         3231 c         2920 g         4336 t
ORIGIN      
        1 gattttcttg agaaaccact cgacctggac cgtctgctca tcactatacg aaatgcaacc
       61 gacaaatcga ggctggtaac ccagacaaag gttttgaaga acaaggtgag caagatgtac
      121 gaaattgttg gtcaatcgcc tgccatactt caggttaagg aaatgattga cagggttgct
      181 cccactgaag ccagagtgct cattaccggt gcaagcggta ccggcaaaga gctggttgcc
      241 caccagattc atgaaaaaag caacagggca aaagggccgt tcgttgaagt taactgcgca
      301 gcaatacctt ccgaacttat tgagagtgaa ctatttggtc atgagaaggg ctcttttacc
      361 tcggctataa gacaacggat agggaaattt gaacaggcaa acggaggtac actattcctc
      421 gatgaaatag gcgatatgag cctgtcggca caatcaaaag tgctcagggc cctgcaggaa
      481 aacaagatca ccagagtggg ctccgacaaa gacatacatg ttaatgtaag ggtaattgcc
      541 gcaacaaata aaaacctgct gaaagaaatt gaacaaaaca ctttcaggga ggacctttac
      601 caccgactca gtgtaatact aatacatgtg cctaccctta atgagcgcct ggaagatata
      661 ccgatgcttg cagaacattt caataccctt atctgcaacg agtatggcat gagtaagaag
      721 attattaccg atgatgccat aaaagaactt cagaaaatac aatggagagg aaatgtaaga
      781 gagttccgga atgtgcttga acgactgatt attctatgca aagacaggat tacagggaaa
      841 gatgttatcg cctacgcgca tccggtttcg ggactaaaac tttaacaata tctcaatttt
      901 aataacagcc aatcagtttt ttaagaactt cgagaagatg gttttttaaa ccattcaggt
      961 tttcttaggt attttcagtt tttgtcctgc ctgtattttt gaaggattac tgatgtcgtt
     1021 agccttgaga acttctgtaa cagtcactcc ctcaaattgt tttactatat cccatattgt
     1081 atctccccgc cgtacagtat agataaaata ttcaccatcg gttatttcgc ttcctgacgc
     1141 aggcaatgca gatgtttgat taaccggaac aggcgttcct ctcagagcct gtttttcagc
     1201 aaaggtcata tcgttaatcc ttgagaaaaa ctcaactttc gaagggtcaa caaacacagc
     1261 aatcttctgt ccaactctga tggtattggt atacagatca ttccagtacc tcaggtcaga
     1321 aagccttact ctgtaccatt ccgctatata tccaaggtta tcaccttctt tcaccgtata
     1381 atataatttt gttttgcctg cgatgctttt tggcgcataa tatgatctga ccggataaac
     1441 ctttgcggta gccctgttca gataaacatc agacttataa tggattattg aatcctgcat
     1501 ctgaatgaaa ccgctcagtg ccgaaagagg aagcctcagg ggttgcggtt ttgtattacc
     1561 agggataagg cctgtcctgt attgtggatt catataacgc aactcatcca ggcttattcc
     1621 cataacttca gagatctgat tcagatgaat atctttgtgc accattattg tatcggttgc
     1681 catcgggatt ttcacaggaa gagggctgat attatgttct ttataataat taatagcata
     1741 tgttgcagct acgaattgag gaatgtaacc gcgtgtttcc cttggcaacc tgtaatatat
     1801 ttcccagtag tcctttctgt ttccggaacg tgctatggct ttgttcacat tacctggccc
     1861 gcagttgtat gctgcaataa caagtaccca gtcgccgaaa atgttgtaca tatctttcag
     1921 gtagcgggcc gctgcatgag tagcctttac aggatccctg cgttcatcca ccagagagtt
     1981 tattgttaat ccgtacatcc gccctgtgct tgccagaaac tgccataaac ctgtcgctcc
     2041 tgctcttgat acggcgtttg gattaagtgc cgactctata actgccatat acttaagttc
     2101 aaccggcagg ccgtacgatt caaaaatatc ttcaatcatc ggaaaataat agtcagcaag
     2161 tcccagaaca gctctcaaaa cttccctttt cctgtcggtg taaacatgaa tatggttccg
     2221 gattatgttg ttatacggaa gattaaccag ggtgctgatt tttgaaaggc gttcactata
     2281 aactgaatcg ggaaattccg gtataacttc ttccatagtg tcagcgggta aattcataaa
     2341 agccgtctgc acataccatg tatgtaaaag gctatcgaga tcccgtgcaa cacgttctgt
     2401 atctgcagtg tcggatttta ttattattgt gtcattaatc tgctgtgcaa aaactgacac
     2461 cggaaaaaac aatattgcaa taatacttat taatttactc ataatcaaaa tgttactaat
     2521 aatttaacat ttatgccggc tcccacaaag ttatataaag gtatcttaac cggctcaatc
     2581 ttcaatgcaa ggctttcgct tatatcatag tcgaacaggc tggcatcgac gctggcatca
     2641 agaatagtaa atagatacca tgctgcgatg ccaatatacg aaagatccct gtattttttg
     2701 aaataatcga tttgtctcat catctggtcc tttatccatg atgcagttga gaggttatag
     2761 gttgaaggat gaagaacggg atcatattct tcgggtttta tattacggat taacttaata
     2821 taactgtcag tttccgggat ctcgtctgta aaatcctgat atgcttttat ataagtatta
     2881 taccattttg tattgtaata tactgcatat ccaaccccgc caaaacctgc ataaacaaaa
     2941 ggcaccttcc agtacttcct gttataaatc tgtcccagtc cgggaaaagc tgctgctagc
     3001 atggtggctt ttgttgggtc gggaatgaat tttcttcttt tgccagttat aacctgtacg
     3061 gaatcctgcg ctgcagcctg ttctgaaaca agaactgcaa agagcaaaac acacaataat
     3121 aatctatgga tgatcccttt caatatatag attttacttt ccggattttt tccggaaaat
     3181 ataacgcagc ggcaaatata attattttca ggagttaaga cggtcaaaaa ggcctaaaat
     3241 acgttccatc tcttcaggag aattgaactg gataacaatt ttaccctttc ctttttcatt
     3301 aatcctgaag acgacctttg atgaaaatat tcgttcgaga tgtgctgaaa gctgcgcaaa
     3361 atcttcgtta agtttctttt tcttctgtgc tttaacaggg tcttttaggt cttccgactg
     3421 cagagttctt accagctctt ccgttgctct tactgaaagg tcttcgtcga taatcttata
     3481 gtagactttt atctgttttt taggatcttc aatattgatc agggtacggg catggcccat
     3541 cgttatctgt ttgttcttta ttccaagctg tatttcggga ggcagtttaa gcaacctcag
     3601 gtagttggca actgtcgacc gttgtttgcc gactctttca ctgagctgtt cctgggtcag
     3661 tttgcattct tccaccagtc ttgaatagct gattgcaata tcaatggcat ccaggtcttc
     3721 gcgctgtata ttttcaacaa gggcaagttc aagcattgcc tggtcgtcgg cagtcctgac
     3781 aaatgcagga acatgcgtca ggcctgccat acgggctgct ctgagtcttc tttccccggc
     3841 gatgagctgg tatcttcccg ttccggtttc tcttaccgta agtggttgta caatacccag
     3901 ttgcctgatt gaagcggcaa gctccccgag cgcctcctcg tcgaaccttg tacggggctg
     3961 aaaaggattg ctgtcaatca gatcaacagg tatctgaaga tttggttcca cctttttctc
     4021 aagtgtctct ttgtcgatac cttcaataag tgctcccagt cctctgccta atgcattctt
     4081 ctttgtcata ttatcgggta atcagttagc ctgcatctgt tttactgtat ctttgtgctt
     4141 atcaataagt tcctgcgcca ggttgaggta attcacagtg cctctcgact ctgcatcata
     4201 cagaatagcc ggctgcccga aactaggtgc ttccgagagc ttcacattgc gctgaataat
     4261 agttttaaaa accatctgct gaaaatgttt gtttacttct tcaacaacct ggtttgaaag
     4321 acgcaacctt gaatcataca tcgtcagcag aaatccttca atttcgagtg agggattcag
     4381 gttcgactga ataattttaa tagtattcaa aagtttcccg agcccttcca gtgcaaagta
     4441 ttcacactga actggaatta ttactgaatc agctgctgtg agtgcattta cggtcagaag
     4501 acccagagac ggcgaacagt caataagtat gtaatcgaac tcattcctta tcccttccag
     4561 caccttttta agcacctttt ccctgccggg catctcaagc atctctatct ctgcgcccac
     4621 aaggtctata tttgagggta taaccatcag attatcgata ccgcacggca taaaagcttc
     4681 ccttggatct ttgccgtcga tgagacaatc ataaattgta acagttatct tcttgacatc
     4741 aacacctatc cccgatgtag cattagcctg tggatcattg tcaacaacaa gaactttttt
     4801 ttcaagtgca gccagacttg ctgccagatt aattgcagtg gtagtcttgc ccactccgcc
     4861 tttctgattt gccagagcaa taatccttcc cattttcctg ttttttcctt tattctttag
     4921 cgttccaaat ttactattta agaaccattg ctgcggactg caggggttgt aattgtaaac
     4981 attttacacg cggttatcaa cacgatatta acaaccagat tgttgataat ccgacactga
     5041 gtttttacat ttttttgttt ttcccggcaa gactctccgc tttttcccac ctgagaaata
     5101 gtgggatggg ggaaagaaag acatttgcaa agctcgaaac tgtaaaggag gtctgttacc
     5161 cggggatgta tgaaattaat tacaaataaa aaatcttaat acctttaccc cactttgttt
     5221 aagccaatga acaacacaga agtccgaagc gactggctcg taattgtcaa tcccaatgcc
     5281 ggaagcggta aaggcggaaa cgactgggaa agaattgctg ctctgctggt gagggcaggg
     5341 atacattttt cggcacgttt taccggcaaa agaggcgatg cgataatgat ttctagggaa
     5401 ggtatcgagc agggattcag aaaaataatc accgtgggag gcgatggcac tctgaacgag
     5461 gtacttaacg gagcttattc acaaaattcc tgcactccgt ctgaagtatg ccttgcactg
     5521 ataccggtgg gaacaggaaa cgactggggc aaaatgttcg gtatacccct tatttacgaa
     5581 ggtgccataa aaattataaa agaaggcaag actttgatgc atgatgttgg agtggtaagt
     5641 taccgaaacg gagaagaaac caatacccgg tactttatta atatagcagg agtcggcttc
     5701 gaggcactgg tggttaaaaa aacaaatatc cagaaagaaa agggtaaaag caacagggca
     5761 atataccttt ataatctgct ttcaagcctt atagcttaca aacataccga agtaactctg
     5821 tatattgaaa aacaaattat taaaaaccgt attttttcaa ttaatgtcgg taacggacga
     5881 tattgcggag gaggtatgcg tcagacgccc gacgctttac ccgacgatgg gttgctcgat
     5941 gttactgtaa taaaagaaat aggaaggatt gaaattatca aaaacctcaa aatactctat
     6001 gacggaacaa tactgagcca tccaaaggtt gacggttaca gggttaaaag cttcagggtt
     6061 gaatcagttc ctcaactgat gattgaagcc gacggtgaac tattgggaca cactcctgcc
     6121 gaattttcag tgataccgga gggcatcaga atagtttacg gatcaaggct cattcagtaa
     6181 cttttatttc aaatagtttt ggccagttct tcccggtgac aaatattctg tcattttcac
     6241 tgtcgtatgc tattccgtta agaacgtttg acgcagtgtc gaatgagggg tcgttcatta
     6301 ttcctttcag gtcaatatat gcaagtacct taccgctggc aggatctatt ctggcaatga
     6361 gatcagtcat ccagatgtta gcccagattt cacctttaat atattcaagc tcattaagat
     6421 cctttaccat ggatttattg tcgtaaacct ccagttgagc cacaggtaaa aacattgccg
     6481 gatcgatgat atacaaaatg ttagaaccat cgctcattac cagtttgtcg tccatagtgg
     6541 taagccccca tccttctgtc tggtaataaa atctatttaa catcctgaac gattcaaggt
     6601 cataaacaaa acccaccttc gactgccatg tcagctggta gattttacca tcgaatattg
     6661 caatcccttc accgaaaagt tttgaatcga ggttatgctg gttcagtact tcacctgtga
     6721 caaggttcac ttttctcaga ttcgacctcg tttcctgacc tgtaccctca taaagataac
     6781 caccgtaata aacaagcccc tgcgtgaatg cctccctgtc gtggggataa acatttatta
     6841 ccctgtaacc gttgcggcgg ggtgcaacat cagaaagaat ggtcacaaat cggcttattg
     6901 tctgagacct gtaccggcgt ttatatgcaa caaccttaat agcttttttc cctgtcttag
     6961 aatttaaaga tgacggtatc tcatattccc aaatattgtt tctgatcacc cccgcatctt
     7021 ttccgtcgaa ccatattctg accgaatcgg gcagaaccct ggcatcaggc tccagcacaa
     7081 ttttaatctt ctcattcagc ttcagctctg cattttcagg cggggcgaca agttttatca
     7141 tctcagctgc catctcctca ggttctgaca actctttaac cggttcattt tctacctcct
     7201 ttttattgga acgtcctgaa catgaaaaga accaggcaac aaacaaaata agcaaagggt
     7261 aaataatgca gttatggcac tttttcatca tctttatttt cttgtccatt ttctgaaaat
     7321 tcttctgaaa atattacctg ctcttaaaat tttccttttt gccatctctt cctgaatctc
     7381 ataagggaac agcatctgcc acacctggct ccatccggga aagccgccaa ggtttttgtc
     7441 atcaatataa atatccacat caattttacg gcttaccgtt tgttcattaa aaacctcttc
     7501 cggataattt ttatttactg catagaactc tataccatgc tgacggcaga attcaactgc
     7561 ttcctcaagt tcttttccag tgcgaaacgt ccaaagtata agctttgccc ctcttttctc
     7621 gagctccttc aatgtactga atgcaaaaag cttttccttt ccgatggaag gataagcatt
     7681 ctcgacaatt gtcccgtcaa aatcaacggc aattctgata ttcagcagat cgaatccgtt
     7741 atccatttta tttaagctct ttcaaagata aaactgattt cattttaata ctccctgtta
     7801 tgctgaaaaa tgtttggaac ggataaaatg atatattatt aatttttcaa tacttttgtt
     7861 ctcaaatcct cagaaatgcg acatatacca aacatgatca cctctttaaa ccttgcatca
     7921 ggttttttat caattatttt tatcattaaa ggaaatacgg cagcagcatg ctggctggtg
     7981 cttgctgcca tggtttttga ttttctcgac gggtttgcat cacgaattct gaatgcatac
     8041 tccgacctgg gcaaggaact cgacagcctt gcagatgtag taagctttgg agtggcaccg
     8101 ggaattatta tttacagtac aggtatattc ttcagcgata ataatgtata cagcataatt
     8161 tacgcagtga tttcggcatt ttatgctgtt tgtgccggtc tgcgtcttgc aaaattcaat
     8221 atcgacacct cacagtcggt tgttttcaga gggcttccta ccccggcagc agccctctca
     8281 gttatatcgc tttgccttgc tgcagcctat tcagataact tttttgtgac gcttttcaca
     8341 aaatatcagt tcttaactat atttttcgtt atcattgtct ctctgcttat ggtaagcaac
     8401 ctgccaatga tttcgcttaa aataaaaaat ttgaagatca aggggaatga ggcaaggtat
     8461 attatggcag ctctgagtat tatttttgtt gcagttttcg gtgccagagg ctttgcactt
     8521 ataataccgg catatattat tctttccctt gcctgggctc cttttctgaa gaatgagatc
     8581 tctcggcatc cctgaacggg tattttcttt cgtatagaat gagtgccgat tttattatta
     8641 tccagcttat gattataaaa gccggccatg taaggtatat cacgagctga tcaaggtaca
     8701 ttggtctata atctttttgt attaataagc atgtccatcc ttttcaatat cttcagctgt
     8761 aattttgcgt tgtgtaacag ccctccatgc ataccagatg tatgcaataa caaatgggat
     8821 aataaatgaa acatacatca tagtttcgag cgtgaacctg cttgaggacg ccctctgcag
     8881 agtaagggaa ttctgaatat cattaacaga agggtaaaag gctgtattgt tcagtcctgc
     8941 agtaagaaac agggcaaaaa cagccataac tgatcctgcg ccggaaaacc agatgccttt
     9001 tctgcttcct gcaaacactg taatataaat accgtaaagc acgcctgcca caccggcaat
     9061 aaacacaatc agtaccggcg gcatctgaag caggtttttc aggtacttaa atttctgaac
     9121 taaaatctct ccggtcgctg gttcaactgc aaatccttcc ttgagaaaaa tcgtaagtat
     9181 gaaataaagg aaaaaaacaa gaaagaagag tgcgttgaaa agtactttgt ttctggcctt
     9241 tgaaattatt tcctcatcat caacagtatt taaaagaaat agtaacccat tcaccctggc
     9301 aagaaaaaga agtgcaaatc ccagagcaat gttccgtatg tcggcaagtg cttcgaggcc
     9361 gtgtaaattt gtctgccagg tgactctgtt caggttgtcg agggagaagt gggcacccgt
     9421 aaaaaatgtt gcaaccgcta tacctgtcag aaatggtccc agtgaaccgt taagaagcag
     9481 gaagatatca taagtattac ttccgaggac atttgagggt ttggatctgt attcgtatgc
     9541 aacagcctga atgacaaaac taaagagtat cagcatccac aaccagtaag caccgccaaa
     9601 acttgtggca taaaaaagtg gaaatgaggc aaaaaaagct ccgccgaaag taaccagtgt
     9661 ggtaaatgta aactcccatt ttctgcccag tgcatttaca atcattttac gctgcatggc
     9721 atttgcaggc aatgaataaa taaaagtctg tcccccctgt acaaacataa gaaaaccgag
     9781 cagaccgccg agcagtgaga tcacaatcca ccagtaatgc tgaagaaatg catgtgacat
     9841 cataactttt cctccgtttt ttcgggtccg ttcttaacct gtttcaccat aatggagatt
     9901 tcagcaataa gaagcgcagt aaacagaacc gcaaagagcc agaaggtggt ttgtacattg
     9961 cctgacttaa ggtttgtaac acccactgat actggcatga gatcctgtat tatccagggt
    10021 tggcggccca tctctgcaag cacccacccc agctcactgg caagatatgg cagaggtatt
    10081 gaaaggagca ttatccacaa aagccattta tgcttccgga tattatttcg gaacaaaaac
    10141 cacagcataa gagcaaacag aagtataaag aagaaaccca gtagcaccat gagatgaaaa
    10201 gtgtaaaaag atacccggat gtcaggtatg atatcttccg gattgtctat ataagcatac
    10261 ccaaaatatc tgaaatagtt ttccctgaag tcttttgagg taaaaagagc ggcaatatcc
    10321 gatgcttttg tctcatcccc tgcctctttt gcctttttat attccagcag tgtttcacga
    10381 gctttctgcc cccgctcttt tttctctgca aatgaaagta ttcctgcccg ggggttccct
    10441 tcaatatggt cataaatacc aggtacataa gcatccttac ttccgccggt cataaatgaa
    10501 agaaggttgg gaatctctat tgcaaatata aactccggca tgttttttct tcccactccc
    10561 ttttctccct ttttgagtat tcctgctgca acaaggggag ccctgctttt cccttcatag
    10621 agtgcttcga acgaagcaaa cttcaccggc tgatattttg ccaccgagcg ggccgacatg
    10681 tcgcctgtga ttgcaagcaa aacagaagaa agaaaaccga atatacctgc tataagaatg
    10741 cttcgtgtag caagatatgt ttcccttttt ttaagcagaa accatgcact tattcccgca
    10801 acaaaaacag aggcaaggac aaatccggaa gtgattgtat gcaggaattt atcggctgca
    10861 acaggattga aaagtacttc cagaaagtta gtcatctcgt tgcgggctgt ttcgggatta
    10921 aaggtcatac cgacaggatt ctgcatccat gcgtttgcaa ccagtatcca caatgccgac
    10981 aggctcgccc ctatagcaac gagccacgtg gaaacaagat gaaacctttt gctcaccctg
    11041 ttccagccaa aaaacatcac agcaataaat gtggattcaa ggaaaaaggc aagtatgcct
    11101 tctatagcca gaggggttcc gaagatatct cccacgaacc aggaatacgc cgaccagttg
    11161 gtgccaaact caaactcgag tattatcccg gtagcaacgc caattgcaaa atttatcccg
    11221 aaaagtttca tccagaactt cgttatcctc ttccattcct cactgcctgt cctgtaataa
    11281 attgtctcca tcatggcaat aatgaatgag aggccaagtg ttagaggaac gaaaaaccag
    11341 tgataaattg ctgtaagggc aaactgcgcc ctggaccagc tgacaagact cagatcaatg
    11401 ttctcaatca tcagatttca ctttgataat aaatgctccc ttatatagtt acttctgttg
    11461 ctgtcgtcag gaaaactttt tttcagaaca tccgggaaaa agaagagcct gagaataata
    11521 aatataatga acaatttaat cagtattatt atccagactt ttctgcccca atctgacatg
    11581 ttcctgaagc catcgagata aaaaacaaat atttttctaa atatgttcag aaatgacata
    11641 gcaatctttc gttgaaaaac tcaaagattt ttcccgtgct ctttttcttc ttcttctttt
    11701 attttctcca ttatttcttc agtatctgga aggcttgagg taattttctg tactgctttg
    11761 aggttataga aaaattcccg ggcaaagact ctcagcacgg tgtatgcagg tatacccaga
    11821 atcatacccg gtataccggc tgcgaagcct gcggcaagaa ccacaacaaa aatctcaagc
    11881 ggatgggctc tgacgctgct ggagaaaata accggctgaa aaacaacatt atctgttgcc
    11941 tgagtaattg caaccactag agccatcttc cataccagtg gaacaataac cgactgaaaa
    12001 tcgtatccca gatgagaggc aatgcccatt gttactgtta tgaaaaatcc aagccacgga
    12061 cccacatagg gaatcacatt aagtatgcct gctataagcc ccattacaag tgcctgatgg
    12121 aaatcgatgc caacaacagt cattccggtt gtcatcatta tcattatgca ggtgctctga
    12181 atcactatcc ccacgaaata ccttgttaaa agcgccttaa ttgaataaag ggcacgggta
    12241 acattatcca catatttgtc gggcacccac ataacgattg attcaaagaa aagatgctgg
    12301 tctttaagaa aaaagaatgt gatgaaactt atggaaaaga atgcaatcac tatgttgtcc
    12361 attgttctgg cggcagagcc tatcatgtcc tgtacaaatt caatgctcag aatatcagtt
    12421 attttcgagg caatgtaagc tctcactgat atctcttcaa gatcaggatt taaagcccgt
    12481 aagaggtttt cagtctttct gataggctcc tcaattatct taacaagttg ttcgctgtcg
    12541 attgaggaaa gttgctgtat ctgactggca acaaggggaa caaatatgta gaaaaagaga
    12601 aaaatcagac cccatataat taaaagtgcc agaagtgcag aaaccgcttt tggaatagat
    12661 atttttctga tctttatccg gtcaaaaaga tcaacaagcg gtctccccat aatggaaaga
    12721 acacctgcaa caagtatgta gattactatt tccctgaagt accagacaca gaaaacaaca
    12781 aatacaattc ctgcaagtat cagaatattt cttataaaat tcttcatctg aagatttttt
    12841 acaaacctaa tttaaaatta tgacaagtca taatagagtt attttcttaa aataactgtc
    12901 atattgacag ttgttttgtc acaaaatatg acatgttgtc atgatttata tattggaaca
    12961 gaagttgcaa gatatagggt gaaattaaac aagaaaaatg tctaaccaaa aataataaaa
    13021 aaagaaagga gggctgagct atgttaccga tgcttacaag aagaagtgac aaaccgttct
    13081 tatggtctga cctctttgat gatgatttct tcttcccgtt ctttacaacc aggtatcatt
    13141 cgatgcctgc tgtaaacatc aaagaggatg agaagaaatt cacgcttgat cttgcagttc
    13201 ctggcattga caaaaaggat ctgaagattg agctgaatga ggatgttctg acaatctcct
    13261 cggagcgcaa ggaggagaag gaagagagcc gtgaggattt caagagaaga gagttcagct
    13321 atgcatcatt ctgcagaagc ttctctctgc ctgaaaatgt tgacagagaa aaaattgaag
    13381 ccagctataa agacggaatc cttacagtga ctcttccaaa gatgcatgaa gagaaatcga
    13441 aggtaacgaa acagatcaag atatcgtaat tctgaggaga atgtcagata caagaaagag
    13501 ggctgctcta agccctcttt ctttttttat tccttattca ggttttttcc ggagcattgg
    13561 gaaagcacaa attctccgaa tctcctgccg tattctttca gtaaacctgc tttttcgttt
    13621 ccggggaaaa atttcaccgt aagtggattt tcaaaatagg ccaatttcag acccttaagt
    13681 gcagctgcaa ttatacccgg agcttcacca ctccatccgt aagaaccaaa agagcctgcg
    13741 ggtttgcctc tgtcgcgcaa aggatttatg aaagcaaaga gtctgtaaac aggcagtagc
    13801 gtattctgat ttatcgttgg agatcctaca agcaatgcat cagctttcat aagctcttta
    13861 tccagctcac ctgctgaaat gttctctata tcaagcaaag ttgcctttac ccccggaatt
    13921 tcttttattc cttcagcaac tatttcagcc atctttcctg taaaaccata agccgaaaca
    13981 taggcaatca aaacgttctt cacttcacct ccagaagtaa tttcgacata ttccctcgac
    14041 aactcttcgg taagcgtaat tatcctttca atatccctgt gatgcacagg cccatgcccc
    14101 gggcagataa acctgatatc cagcggtttt attttttcga ttgccttaag tgcaaagcgg
    14161 ctgaagggcc tcaggataac atcaaaataa tacttaaaag acttaaaata atcctccgaa
    14221 aaatcattga acatctcttc tgaacagtag tgagctccga acatatcgca ggtaaagagt
    14281 accctgtcct cctcaagcca tgtaaatatt gaatcgggcc agtgaagatt cggtgcagct
    14341 ataaaccgta gtgttttgtt gccaaggctg agggtatcgc catctttgac agcaattgat
    14401 ttgaatggct tatttatgat atctgaaaga taccttatca tatttccgct gccaacaacc
    14461 gtggcatcgg gcgccagttc aagcagtctt ccggtgttgc ccgaatggtc gggctcggta
    14521 tggtcaagaa ttatatattg aatttcggaa ggatcgcaaa gcttctttaa cttgcttatc
    14581 tgaacatcgc tgaacctgtc cttggcagct tcaaccacag cctttttctc agcattgata
    14641 aaatatgagt tgtatgttgt gccaaactct gtctccatca caatatcgaa agtaacgata
    14701 tcgtaatcga gtactcctat ccactttaca tcgggagtaa tttctataat tctgtcatcg
    14761 ggtttacggt ctttactttt actttcactt ttcatagccg tgcaattatg cttttgtttg
    14821 ccctgacgtg ttgaagtaaa ggtacttcca ctt
//
LOCUS       DAOSNB010000024        14801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4078_length_14801_cov_5.222026, whole genome shotgun sequence.
ACCESSION   DAOSNB010000024 DAOSNB010000000
VERSION     DAOSNB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4078_length_14801_cov_5.222026"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLG42_02095"
     CDS             <1..404
                     /locus_tag="PLG42_02095"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOU30033.1"
                     /translation="GGGYYNHILFWENMAKGLSTPSSEMVKALESAFGSYDRFKELFS
                     NAAKTHFGSGWAWLYLTTDKKLAVGSTPNQDSPVMDVSTIKGTPLLTIDVWEHAYYLK
                     YQNRRAEYVDNFWNVVNWQEVEKRYRKALTL"
     gene            complement(531..2126)
                     /locus_tag="PLG42_02100"
     CDS             complement(531..2126)
                     /locus_tag="PLG42_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU30034.1"
                     /translation="MKKNGDRNALSFIDEKPLTYKEVDQKINSLINFLEKNNIKKGDR
                     VAILGTNMPNWGISFYAVTFKGAVAVPILPDFSKSEIQNVINHSESKLLIISSSLLSK
                     IEHLDGNQLKTVVLMENFLPVKTSGEFVAYSENSRPSEKYSVNCEDLASIIYTSGTTG
                     KSKGVMLSHGNITFNAIAGSKIHYLDENDRFLSVLPLSHTYENTLGLILPMLRGACVY
                     YLRKPPTPAVLLPALGKVRPTAMLTVPLIIEKIFFNKILPSLRKNIMIRSIYKIPPIR
                     KLLNRLAGKKLMKTFGGELRFYGIGGAKLNSTVEKFLIEASFPYAIGYGLTETSPLLA
                     GAGPANSVYESTGPSIEGVELKIHNPDPVTGEGEIWAKGPNVMIGYFKDPELTAEVIT
                     SDGWFKTGDLDVFDKRNNLYIKGRAKNVIVGASGENIYPEEIESLINSFRHVVESIVI
                     EQKGKLVALVHLNIEEIEKKYNIMKNEASRRFEEKKEEILREIQEYVNSKVNKFSRIS
                     KVVYQPLPFEKTATQKIKRYIYK"
     gene            2335..2778
                     /locus_tag="PLG42_02105"
     CDS             2335..2778
                     /locus_tag="PLG42_02105"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOU30035.1"
                     /translation="MPVIRITREFTIEMAHVLEFHYGQCKNVHGHSYRLFVTVAGTPL
                     NEPGSPGDGMIMDFAELKKIVEEKVINIFDHSLVVSKSFEKRYNNIRGIFTNLVVLDY
                     QPTCENLVIDFAERIKSGLPDGIYLHSLKLYETSKSYAEWYSSDN"
     gene            2791..5589
                     /gene="polA"
                     /locus_tag="PLG42_02110"
     CDS             2791..5589
                     /gene="polA"
                     /locus_tag="PLG42_02110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOU30036.1"
                     /translation="MSESGEKKLFLLDAYALIFRSYYAFIKNPRITSKGLNTSAIFGF
                     LLTLEEVLQKQKPTHIAVVFDSSEPTFRHKMYSEYKATREETPEDIKKAVPYIKQLVE
                     AYRIPILECPGFEADDIIGTLANKAAQKGFVTYMMTPDKDFAQLVSDRIFMYKPSRAG
                     NESVIWGIKDVKREFNVKSPEQVIDILGLMGDTSDNIPGAPGVGPKTAMKLISEFGSI
                     EELLKNTDKLQGRIKEILEKHKEQVLLSKKLVTINTNTPVEFDEESLKLEPPDKEKLK
                     ILFDELEFRQTASRILSESTENKDTLVVEQETKTSPETIQGTLFGEAAVSETKLNIEL
                     VPHSYNLIQTREDLKKLAEELSKLEEFCFDTETTSINALESELVAIAFSWIKGKGYLV
                     YFPENENEIKEMLSILKPVFENDRIRKIGQNIKFDIQVLANYGIEVRGALFDTMIAHY
                     LLEPDMRHNMDYLAETYLSYRPVSIESLIGERGRNQKTMRSVPVEKIVEYSVEDADIT
                     LQLKELFEPKIIKEGMKELAENIEMPLIRVLACMERAGVKLDEENLKSIAVGLREDII
                     RLEGEIYALAGEEFNISSPRQLGNILFIKLKIDDKAKLTKTKQFITNEEILQKLVNRH
                     PIVGKVLEYRGLKKLLTTYVETLPLLINKKTGRIHTSFNQAVAATGRLSSNNPNLQNI
                     PVRDERGREIRKAFVPEKGYIFLSADYSQIELRLMAHLSGDKNMIEDFISGHDIHTAT
                     AAKIFGVSFDEVTREMRSRAKTANFGIIYGISSFGLSERLNIGRKEAKELIDGYFASY
                     PGVKQYMEESIKQAREKGYVTTMFGRRRYVRDINSRNQVVRGNAERNAINAPIQGSAA
                     DIIKIAMIRIFDQIRNKGLKSRMILQVHDELILEVAHDELEMVKEIVISEMTGAASLK
                     VPLKVDWGTGSNWLEAHP"
     gene            complement(5596..7263)
                     /locus_tag="PLG42_02115"
     CDS             complement(5596..7263)
                     /locus_tag="PLG42_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU30037.1"
                     /translation="MKKQTGKKILILITIVLFAVTTTAFLINQESRDFRLGKNLDIFI
                     SLIRELNTFYVDEIDPDKIIRTGIESILRSLDPYTTFYSESEIDDFNFMTTGKYGGIG
                     CLIRNNGDYAIVTEVYKGFPADLAGIKAGDLIKKIDGQSLKGFAVDKVSDNLKGDPGT
                     TVSVTVERNGKEIEFQLKRERISLPPVPYYGIVDSNIGYIRFTNFTQNCYEDVKKALI
                     ELKKNNPDGIILDLRSNPGGIMMEAVDIVNLFVGPGTEVVSTKGKVKQFDASYKTKNQ
                     PVDDRIPLVVLINRMTASASEIVAGAIQDLDRGVVVGQRSYGKGLVQITRPLSFNAQL
                     KVTTAKYYIPSGRCIQAIDFTHRNEDGSVGYIPDSLIKTFKTRNGRPVKDGGGITPDI
                     EVVPENLSQLATELYTRNLIFDYATKYYWSHPEIKSIEDVKITDNEYEDFIKFLDERG
                     FNYQTFTEKSFSELVENAKKEKYFEPNKELFNTLQKEITHNLRRDLQSFKEEISELLC
                     DEIISRYFYEEGAIAWSIKYDEQVHKAIEVLNNKSLYRSILEGKPAR"
     gene            complement(7382..>7735)
                     /gene="rnpA"
                     /locus_tag="PLG42_02120"
     CDS             complement(7382..>7735)
                     /gene="rnpA"
                     /locus_tag="PLG42_02120"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOU30038.1"
                     /translation="KKAIELLFEKGISVISYPFQVIWTFSNQDSAAPAKIAISVSKKL
                     FRSAVKRNAIKRKIREAYRRKKYILYDFLTSTGKSINFILIYRDRRIPDFREIEKHVD
                     DVLRKLVSSVSTSKE"
     assembly_gap    7736..7835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7839..8597)
                     /locus_tag="PLG42_02125"
     CDS             complement(7839..8597)
                     /locus_tag="PLG42_02125"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOU30039.1"
                     /translation="MKIKKILVSQPDPQNPKSPYHELAKKYNLKVDFKPFVQIEGVTA
                     KDFRQQKINILDHTAIIFSCKTAIDHFFRICNELRIAVPDTMKYFCITENVAFYLQKY
                     IVYRKRKIFHGKSKFQDLIDVIIKHKDENYLVPLPEPHNSEIPELLKKNNIKHTIGIF
                     YKTISNHFESINDLLDYDIIVFYSPSSIKSLRENFPDFVQGNIKIAAFGPNTVMALEQ
                     EGFRVDIQAPNPKSPSMTMALDEYLKEYNKNHNK"
     gene            complement(8627..9691)
                     /locus_tag="PLG42_02130"
     CDS             complement(8627..9691)
                     /locus_tag="PLG42_02130"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOU30040.1"
                     /translation="MPVKMVPQDTLKLKQADGTYLTGQKQDTLPRKNADSVKTISLDT
                     TVKKVISKIHQDVKAQNKEKIDTSIENKKLSTDFTNKKTSDIINYYLSDYETITSEGP
                     NICFPYSFIESSQKLKKDLEIKISEVAKDGIKIEKDTLKSDWMLPVMLISLLVYLILG
                     RAFSDSMKLIKKFFTFRIAHDSGTTKTLRFSSTLLELISYVNISLYGYLLFTYFNIGT
                     GVIKGYFAWIIIFISTIFLSLARQSVTFLTGEISEQKEIFMEYGRAISCFYRSTGLIL
                     FFIIIILLYTNFFNTKTIIYSGFFIVIFIYVIRIVNLFTIFIKRSASILYLILYLCAL
                     EILPVAILIKFLKQLLQNIQ"
     gene            complement(9731..10603)
                     /gene="deoC"
                     /locus_tag="PLG42_02135"
     CDS             complement(9731..10603)
                     /gene="deoC"
                     /locus_tag="PLG42_02135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOU30041.1"
                     /translation="MTKLYEKLFKSCPSEGEIKKGLKKVYSGISGDPDRKTLMEILSV
                     IDQTSLNTTDNKSSILQLTGKINSFNARYSNIPSVAAICVYPNFVPLVKEKLTARNVK
                     IAAVAGGFPASQTFRSIKVAECKMAADAGADEIDFIFNVGAFLGNDYQTVADEIREIK
                     SNIGKAYLKVIIESGLLHNIEQIFNASMIAMDAGADFIKTSTGKTSVSATPESVFVIC
                     RAISVFYNETGIRVGIKPSGGIASSKDAIVYYNIVKHNLGAEWMNPSLFRIGSSRLAN
                     DVLSEIIGSRVDYF"
     gene            complement(10704..12110)
                     /locus_tag="PLG42_02140"
     CDS             complement(10704..12110)
                     /locus_tag="PLG42_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU30042.1"
                     /translation="MTKREQGIPGDKSLQKSVLLVATFASFLNPFLGSAINLALPAIE
                     KDLNTDAVELGWIVSSFILTSAIFLLPMGKLADIRGRIKIFRAGIFLFTLSTFFIIFC
                     RDIHSLIIMRIFQGLSGAMFFGTSLAIITSVFPPGERGKAMGINITAVYLGLSAGPIV
                     GGFLTREFGWRSIFVFLVPIGIIAQILISRKIKTDWAEASGEKFDWKGSILYGISLAL
                     FMYGFSKLPSFCSWICLSSGILAGIFFILFEKSVNYPVFDIKLFLHNRVLTFSGIAAL
                     IHYAATSAIGFFMSLYLQYIQNLDARSAGFIMIAQPLAMMILSPAAGKLSDRKNPGVI
                     ASIGMAITATSLVSLCFLRDNTPLFLIIILLGITGTGFAFFSSPNSNAIMSSVEKKYL
                     GVASATVGTMRMIGQMTSMGIAMMLLALFIGKQQISPSTYKGLMAGMRTGFMIFSVLS
                     IIGIFASLARNKSLNRKS"
     gene            complement(12111..12566)
                     /gene="dtd"
                     /locus_tag="PLG42_02145"
     CDS             complement(12111..12566)
                     /gene="dtd"
                     /locus_tag="PLG42_02145"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOU30043.1"
                     /translation="MRVVIQRVKRASVTVDGELISQIGPGLLVLAGFEDADTEEDIEW
                     LSNKIVNLRIFNDNEGVMNLSVKETEGNILVVSQFTLHALTKKGNRPSYIRAAHPDKA
                     IPLYEKFIIALSERLGRKTASGKFGAMMDVELINDGPVTIIIDTKNKDF"
     gene            complement(12628..14229)
                     /locus_tag="PLG42_02150"
     CDS             complement(12628..14229)
                     /locus_tag="PLG42_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30044.1"
                     /translation="MVRLIVFLIIAISLLSCKKSDNSVFKEASDNSILSNEGFNRCIA
                     YVNAWSRHADPESGLIPRNLRESIDYWNAWDAAADNYPFMVLTSSLLMPDFFNTAAVK
                     MLENERKLTSRIGKLPDTYSFSRKGFRNEAIDTSQIIFGSAEYMKDGLIPITEWLGKS
                     IWSDRMLEILEDFQKIATVVKELKGSSFGRNAVVEVNGDLLQTLSRMYWFTGNRKYLE
                     WAAEIADYYLSDENLITQHSDHIRIRDHGCEILSGLCEAYLAAYYVWPEKRAQWRKNL
                     HIMLNRILEVGRNEDGLFYNEINPQTGEIISDGIADTFGYTLNAYYFIADIDSVPEYR
                     EAVIKALDILYDNYRNFNWENGGCDGYADAIEGALNLYNRIPLPKLKKWIDSEIRIMW
                     NYQKPDGIIEGWHGDGNFARTTIMYCLWKTQGIIPDHWDEKLKIGAVYESGQLIIAAS
                     CENVWEGKLQFGIPRYKEKMHLPVDYPRINQFQQWFIPEYDKVYKVFFKPGNKMKKFS
                     GRELIEGMPLKLIPGEEIFITIKSK"
     gene            14355..>14801
                     /locus_tag="PLG42_02155"
     CDS             14355..>14801
                     /locus_tag="PLG42_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HOU30045.1"
                     /translation="MDFVTILLIALGLSFDTFAASVVFGITCSRIVLAQAIRVAIVMA
                     LFQGGLTVTGYFAGTVISGPLKLLDHWIALILLLVIGIRMITGGIQKKQHLKPRDYTR
                     TPELIAIALSTSIDAAAVGISFALLQARIWFSGAVIAIVTFLASMTA"
BASE COUNT         4447 a         2633 c         2830 g         4791 t
ORIGIN      
        1 agggtggcgg atattataat catattcttt tctgggagaa tatggcaaaa ggattgtcca
       61 caccatcatc cgaaatggtg aaagcattag aatcagcttt tggatcttac gacaggttca
      121 aagaactatt cagtaatgct gcaaaaacac actttggaag tggatgggca tggctttatc
      181 ttacaaccga caaaaaactg gctgtgggga gcactccaaa tcaggatagt cctgtaatgg
      241 atgtttcgac aataaaagga acgccgcttt taacaataga tgtctgggaa catgcctatt
      301 atctcaaata ccagaacaga cgtgctgaat atgtcgacaa tttctggaat gttgttaact
      361 ggcaggaagt tgaaaagcgt tatagaaaag ctttaacgct ttaatgagag tttttacctg
      421 ttgtgctgat aaaaatatca ggtttttaag aggctttcta aaaagcctct tttttgtttt
      481 aactcctgag tcccctgaga aaggacatat tgatatacaa tattttatct ttatttataa
      541 atatatcttt ttattttctg ggtggctgtt ttttcgaatg gtaaaggctg atatacaact
      601 ttgcttattc ggctgaattt attgactttt gaattgacat attcctgaat ctccctgaga
      661 atttcttcct ttttttcttc aaaccgcctt gaagcttcat ttttcataat gttgtatttc
      721 ttttcaatct cctcaatgtt aaggtggaca agggcaacga gtttgccctt ttgttcgatt
      781 acaatagatt ctacaacatg tctgaagctg ttaatcagtg attcgatctc ctcaggatag
      841 atattttctc cgcttgctcc tacaattaca ttttttgccc ttcctttaat gtatagatta
      901 ttccttttat caaacacatc aaggtctcct gtcttaaacc acccatctga agtaataact
      961 tctgctgtta gttccggatc tttaaagtag ccaatcatta catttgggcc ctttgcccat
     1021 atttcacctt ctcctgttac tggatcagga ttgtgaattt taagttcaac tccttcaata
     1081 gaaggacctg ttgattcata aacagaattc gcagggccgg caccagctag aagaggcgat
     1141 gtttcagtaa gtccatatcc aattgcatat ggaaaagatg cttctatgag aaatttttca
     1201 acggtgctgt taagcttggc tccacctatt ccgtaaaacc tgagctctcc tccgaatgtt
     1261 ttcattaatt tcttgcctgc caaacggttg agaagttttc gaataggagg tattttgtaa
     1321 atagagcgga tcattatgtt tttacgaaga gatggcaata ttttattgaa aaatatcttt
     1381 tcgataataa gtggaactgt caacatggct gttggtctga ccttgccaag tgcaggaaga
     1441 agtactgccg gggttggtgg ctttctaagg taataaacac aggcccccct gagcattgga
     1501 agaatgaggc cgagggtgtt ttcgtatgtg tgcgacagtg gaagtaccga aaggaacctg
     1561 tcgttttcgt caagataatg gattttgcta cccgcaatgg cattaaatgt tatgttacca
     1621 tgcgaaagca ttactccttt agactttccg gttgttccgg atgtataaat tattgaggca
     1681 aggtcctcac aattgacgga atatttttcg gatggccttg aattttctga gtaagcgaca
     1741 aattctcctg aagttttgac tggtaaaaaa ttttccatca atacaactgt cttcagttga
     1801 ttaccatcca ggtgttcaat ctttgacaaa agtgaagatg atattattaa aagtttagat
     1861 tcagaatgat ttatcacatt ctgaatttct gattttgaaa aatcgggtaa tataggaact
     1921 gctacggcgc ctttaaaggt gaccgcataa aatgaaatac cccagttggg catatttgtt
     1981 ccaagaatag caactctgtc accttttttg atattattct tttcgagaaa attaataagt
     2041 gagttgattt tctgatctac ttctttgtat gtcaatggct tttcgtctat gaaagataaa
     2101 gcgtttctgt cgccattttt tttcaaagtt tctgagaaca aagaaggaat tgtggtggga
     2161 atgataaaag aattcattaa actatttaaa attgcaaaga taagttttta tttttaatta
     2221 ccttgaagta taaggaaaat atttatgtga agcacatgaa aaagcgtcaa aatcattttc
     2281 tttcctttca accagaatag ttatttttgt aagtgtaatt caatattaat acctatgcct
     2341 gtaatcagga taacacgcga attcactatc gaaatggctc atgtacttga atttcattat
     2401 ggacaatgca aaaatgttca tggacattca tacaggctgt ttgtaactgt ggcaggaact
     2461 cctttgaatg aaccgggaag tccaggagac ggaatgataa tggattttgc tgaattgaaa
     2521 aaaatagtgg aagaaaaagt tataaatatt ttcgaccatt cacttgtagt cagtaaaagc
     2581 tttgaaaaga ggtacaacaa tatacggggg atatttacca accttgtagt actggattat
     2641 caacccacat gcgaaaatct ggttattgat tttgcagaaa ggattaaatc tggtctgccc
     2701 gacggaattt atcttcattc attgaagctt tatgaaacgt caaaatctta tgctgagtgg
     2761 tactcttctg ataattaata ttacctgttt atgtcggaat ccggtgaaaa gaaactattc
     2821 ctgcttgatg cttatgcatt aatattcaga tcttattacg catttatcaa aaatccgcgg
     2881 ataacttcaa aaggcctcaa tacgtctgca atttttggat ttcttctgac cctcgaggaa
     2941 gttttacaga aacagaagcc aacgcatatt gcagtagttt ttgacagttc tgagccaaca
     3001 ttccgccaca aaatgtacag cgagtacaaa gctacgaggg aagagacacc tgaggatatt
     3061 aagaaagcgg ttccatatat taaacagctt gttgaagcat atagaattcc cattctcgaa
     3121 tgtccgggtt ttgaggcgga tgatattata ggcacactgg caaacaaagc ggcccaaaaa
     3181 ggctttgtaa cttatatgat gacacctgat aaagatttcg cccagcttgt ctccgacaga
     3241 atttttatgt acaaaccttc gagggcagga aatgaatcgg ttatatgggg tattaaagat
     3301 gtgaaaaggg aattcaatgt taaatcccct gaacaggtca ttgatattct gggacttatg
     3361 ggggatactt cagataatat tcctggtgct ccgggtgtgg ggcccaaaac tgcaatgaaa
     3421 cttatttcag aatttggaag tattgaggaa ttgctaaaaa atactgataa gttgcagggc
     3481 aggataaagg aaatacttga aaaacataag gaacaggtac tgttgtcgaa aaagctggta
     3541 acaataaata caaatacccc tgttgaattt gatgaagaga gccttaagct ggaaccgcct
     3601 gataaagaaa aactcaaaat attgttcgat gagcttgaat tcaggcaaac ggcttcaaga
     3661 attctttcag agtcaactga aaataaagat actttagtag ttgaacagga aacgaaaacg
     3721 tctcctgaaa caatccaggg aacacttttc ggagaagctg ctgtttcaga aaccaaactt
     3781 aatattgaac ttgtaccaca ttcttacaat cttattcaga cacgggagga tctgaagaaa
     3841 ctggcagaag aacttagcaa acttgaggaa ttctgttttg atacagaaac tacaagtatt
     3901 aacgcacttg aatctgaact tgttgctatt gcattttcat ggattaaggg caaaggttat
     3961 cttgtatatt tccctgaaaa tgagaatgaa ataaaagaaa tgctttctat tctgaaacct
     4021 gtgtttgaga atgacaggat acgtaaaata ggtcagaata taaaatttga catccaggtt
     4081 ctcgcaaatt atggaataga agtcaggggc gcacttttcg acacaatgat tgctcattat
     4141 ctccttgagc ctgacatgag acacaacatg gactatctgg ctgaaacata tctttcttac
     4201 agacctgtct caattgaaag cttaataggt gaaagggggc gaaatcagaa aaccatgcgt
     4261 tcggtaccgg ttgagaaaat agttgaatat tctgttgagg atgccgatat aacattgcag
     4321 ttaaaagagt tgtttgagcc aaaaattatc aaagagggca tgaaggaact ggcagaaaat
     4381 attgagatgc ccctgatcag ggttcttgcg tgtatggaga gagccggcgt taaacttgat
     4441 gaagaaaacc tgaaatcaat tgctgtaggt ttgagggaag atattatcag actggaaggg
     4501 gagatttatg cactggctgg tgaggagttt aacatttctt ctccccggca actgggaaat
     4561 attcttttta ttaagctaaa aattgatgat aaagcaaaac ttaccaaaac aaagcagttt
     4621 attacaaatg aagaaatcct tcagaagctt gtgaacagac atcctattgt tggtaaagta
     4681 ctggaatata gaggattgaa aaaattactt acaacttatg ttgaaacact gccattactt
     4741 attaacaaga agacagggcg aatacataca tcgtttaatc aggcggttgc agctacaggc
     4801 aggctaagtt caaacaatcc gaatctacag aatattccag tcagggatga aagaggacgg
     4861 gaaataagaa aagcttttgt acctgagaaa ggttatattt tcctgtctgc agattattcc
     4921 cagatagaat tgcgattgat ggcacatctg agtggtgata aaaatatgat tgaggatttt
     4981 atatcggggc acgatattca tactgctact gctgcgaaga tttttggtgt cagttttgat
     5041 gaagtaacaa gggaaatgcg aagcagggca aagacagcta atttcggaat tatatatgga
     5101 atttcatcgt ttggactttc tgagaggcta aatatagggc gcaaagaagc aaaagaactt
     5161 attgacggat attttgcatc atatcctggt gtaaagcaat atatggaaga aagcataaag
     5221 caggccaggg aaaaggggta tgtaacgaca atgttcggaa ggagaagata tgtaagggat
     5281 attaattcac gcaaccaggt ggtaagggga aatgctgaac gtaatgctat taatgctcca
     5341 attcagggtt cagcagcaga tattatcaaa attgccatga taaggatttt tgaccagatt
     5401 cgcaataaag gcttaaagtc gaggatgata cttcaggtac atgatgaact gattctggag
     5461 gttgcccatg atgagcttga gatggtaaaa gaaattgtta tcagtgaaat gaccggggca
     5521 gcttcattga aggtgccttt aaaagttgac tggggtacag ggtcaaactg gcttgaagca
     5581 cacccctgag caaaattacc tcgctggctt tccttcgaga attgacctgt atagtgattt
     5641 attattaaga acttctatgg ctttatgtac ttgctcatca tattttattg accatgctat
     5701 tgccccttcc tcataaaaat atctgcttat tatttcatcg cataggagtt ctgaaatctc
     5761 ttccttgaaa ctctgaagat ctcttctgag attgtgagta atttctttct ggagggtatt
     5821 gaaaagttct ttgttgggtt cgaagtattt ttctttcttt gcattttcaa ccagttcaga
     5881 gaatgatttt tcagtaaatg tctgatagtt gaatcctctt tcatcaagga actttataaa
     5941 atcctcatat tcgttatcgg ttattttcac atcttcaatg ctttttattt caggatgtga
     6001 ccagtaatat tttgtagcat aatcgaaaat caggttacgg gtatacaatt cagttgcgag
     6061 ctgactgagg ttttcgggta caacttcgat gtcgggagta attccgcctc catcttttac
     6121 cggccggcca ttacgtgttt tgaaagtttt tatgagtgaa tcggggatat atcctacact
     6181 accatcttca ttacggtgtg taaaatctat agcctgaata caacgtccgc tgggtatata
     6241 gtattttgcc gttgtaactt ttaactgtgc attaaagcta agcggacgtg tgatctgcac
     6301 aagacctttt ccgtaggatc tttgaccgac aactacacct cggtcgagat cctggatggc
     6361 accggctacg atttctgaag ctgatgctgt catcctgttt ataagcacaa ccagaggaat
     6421 tctatcatca acaggctgat tttttgtttt gtacgaggca tcaaattgtt ttactttgcc
     6481 ttttgtgctc acaacctcag ttccgggacc aacaaaaagg ttaacaatat cgactgcctc
     6541 catcattatc ccgccgggat tagacctcag atctagaata ataccatcag ggttgttctt
     6601 tttaagttca ataagagctt ttttaacatc ttcgtaacag ttctgggtga aatttgtaaa
     6661 tctgatatac ccgatatttg aatcaacaat tccataataa ggaacaggag gaagtgaaat
     6721 tctttctctt ttcaactgaa attcaatttc tttcccgttt ctttcgacgg tcaccgaaac
     6781 agttgtcccc ggatcacctt ttaagttgtc gctaacttta tcaacagcaa aacctttaag
     6841 tgattgtccg tcaatctttt taataaggtc tcctgcttta atccctgcca gatctgcagg
     6901 aaaacctttg tatacttctg ttactattgc ataatctcca ttatttctta tcaaacagcc
     6961 tatcccacca tatttcccgg ttgtcataaa gttaaagtca tcaatttcag attctgaata
     7021 gaatgtagtg tatggatcaa gagatctgag gatgctttct attcctgtcc tgataatttt
     7081 atcaggatct atttcatcaa cataaaaagt attcagttca cggatgagtg aaataaaaat
     7141 gtcaagattt tttcccagcc tgaaatccct ggattcctgg tttatcagaa atgcagtggt
     7201 ggttacggca aacagaacaa ttgtaatcag aattaatatt ttctttcctg tctgcttttt
     7261 catgatctgc ttcatcgtta acaaatatat tttgataaaa gacgaaaata gaaagaaatt
     7321 atttcagatg gattgtattt atgttttata agaaaacttt aacattgatg tctttgggtt
     7381 tttattcctt gcttgtactt acagatgata caagtttccg gagtacatca tcaacatgct
     7441 tttctatttc ccggaaatcg ggaattctcc tgtctctgta gattaaaatg aaatttatac
     7501 tcttacctgt cgatgttaga aaatcataaa gaatatattt tttccttctg tatgcttccc
     7561 tgatttttct ttttatagca tttctcttta cagcagatct gaagagcttt tttgaaacgc
     7621 tgattgcaat ctttgccggc gcagctgaat cctgattgct aaaagtccat attacctgaa
     7681 aaggataact tattactgaa atgccttttt caaaaagtaa ttctatagct tttttnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngagct atttgttatg gttcttgttg
     7861 tattccttca ggtattcatc aagagccatt gtcattgacg gtgattttgg atttggtgcc
     7921 tggatgtcga ctctaaatcc ttcctgttcg agtgccatta ctgtgtttgg tccgaaagct
     7981 gcaattttaa tatttccctg cacaaaatcg ggaaagtttt cgcgaagtga ttttatgctt
     8041 gaagggctgt aaaaaacaat tatgtcataa tcaagcaagt catttatact ttcaaaatga
     8101 ttgctgattg ttttgtaaaa tattccaatt gtatgtttaa tgttgttttt cttaagcagt
     8161 tcaggtattt ctgaattgtg gggttcagga agaggtacca ggtaattctc gtctttgtgt
     8221 ttgattatta catcaatgag gtcctgaaat tttgattttc catggaaaat tttgcgtttg
     8281 cggtatacga tatatttctg caagtaaaat gcaacgtttt cggtaatgca gaaatatttc
     8341 attgtatcag gaacagcaat gcgaagttca ttacatatcc tgaagaaatg atcaattgct
     8401 gttttacatg aaaaaattat ggcagtatga tccagaatgt ttatcttctg ctgcctgaaa
     8461 tcctttgcag taactccttc aatctgaacg aaaggcttaa aatcgacttt caggttatat
     8521 ttctttgcca gttcatgata aggagacttt ggattctgcg gatctggctg cgacactaaa
     8581 atttttttta ttttcaaccc cttacagatt tagtttgaca cctgtatcat tgaatattct
     8641 gcaacagttg ctttaaaaat ttaataagta tagcgactgg cagaatttcc agcgcacaaa
     8701 ggtataaaat taaatacaaa attgatgcac ttctctttat aaaaattgta aaaaggttga
     8761 ctattcgtat aacgtagata aatatcacaa tgaaaaatcc tgaatagatt attgttttcg
     8821 tattgaaaaa gttagtataa agaagaataa ttattataaa aaacagaatt aatccagtgc
     8881 ttctgtaaaa gcaggaaata gccctgccat attccataaa aatttctttt tgttcactta
     8941 tttcaccggt aagaaaggtc actgattgtc tggcaagtga gagaaaaatt gttgaaataa
     9001 aaattattat ccatgcaaag taacctttta ttacaccagt accgatattg aaatatgtga
     9061 agagcagata cccgtaaagg ctgatgttta catatgatat cagttcgaga agtgttgatg
     9121 aaaatcttaa agtttttgtt gtacctgagt catgagcaat cctgaatgta aaaaatttct
     9181 taattaattt catggaatct gaaaatgctc tgcccaatat tagataaacc agcaatgaaa
     9241 tgagcatcac tggcagcatc cagtcagatt tcagtgtatc tttctcgatt ttgatgccat
     9301 ctttagctac ttcagaaatt tttatttcca gatctttttt aagcttttgt gacgattcaa
     9361 tgaaggaata agggaagcaa atatttggcc cttctgatgt aatagtctca taatcagata
     9421 aataatagtt tattatatca gaagtttttt tattagtgaa atcagtactt aattttttgt
     9481 tttcaataga agtgtcgatt ttttccttgt tttgtgcttt tacatcttga tgaattttgg
     9541 aaatgacttt ttttactgtg gtgtcgagtg atattgtttt tactgaatct gcattttttc
     9601 tgggaagggt atcctgcttc tgtccggtga ggtaggttcc atcggcctgt ttaagtttta
     9661 gtgtatcctg aggtaccatt tttaccggca ttattaaaca attttcaccg gcttttatga
     9721 gttgccggct tcagaaataa tctactcttg agccaattat ttctgaaagg acatcattgg
     9781 ctagacggct tgatcctatg cggaaaagtg aaggattcat ccactctgca ccgagattat
     9841 gtttaacaat attgtaatac acgatggcat ccttggatga tgctatacct cctgaaggct
     9901 taatcccaac acgtatgccc gtttcattat aaaatactga aatggcacgg cagattacaa
     9961 aaacggattc tggtgtagca gagacacttg ttttaccggt tgaagtcttg ataaaatctg
    10021 ccccggcatc catcgcaatc atcgaagcgt taaatatctg ctcaatgttg tgaaggagtc
    10081 cggattcgat aatcactttg aggtaagcct tccctatgtt acttttgatt tctctgattt
    10141 catctgcaac tgtctgatag tcattgccga ggaaagctcc cacattgaaa ataaagtcta
    10201 tttcatcagc tccggcatct gctgccatct tacactcggc tactttgata cttctgaatg
    10261 tttgtgatgc tggaaatcct ccggcaacag cggctatttt tacatttctt gcggtaagct
    10321 tttcttttac caggggtacg aaattcggat aaacacatat tgcagcaaca gaaggaatat
    10381 tggaatatct tgcattaaag ctgtttattt ttccggtgag ttgtaatatg ctgcttttat
    10441 tatcagtagt gttgaggctt gtctggtcaa ttacactgag aatctccatc agtgtttttc
    10501 tgtcggggtc tcctgaaatt cctgaatata cctttttcaa ccctttttta atttctcctt
    10561 cagaaggaca cgatttaaaa agtttttcat acagttttgt cataaggcat ctgatttaaa
    10621 ataagttaga tattattcaa cgattaaaaa gcctgatggc aatatataat gtttttctga
    10681 tttattgagt tgtatctgaa aatttatgat tttctgttaa ggcttttgtt ccttgccagt
    10741 gatgcaaaaa taccgattat ggaaagaaca gaaaaaatca taaaacctgt tctcatacct
    10801 gccataagtc ctttgtaggt ggatggcgaa atttgttgtt ttcctataaa taatgctagt
    10861 agcatcattg caattcccat acttgtcatc tggcctatca ttctcattgt gccgacagtt
    10921 gcagaggcaa cacccagata ttttttttcg actgaactca taattgcatt tgaattgggt
    10981 gatgaaaaaa aagcaaatcc cgtacctgtt atcccgagaa ggataattat taagaaaagt
    11041 ggtgtgttat cccgtaaaaa acaaagagat acaagacttg ttgcggtaat agccatacca
    11101 atggaagcaa taactcctgg atttttcctg tcggatagtt tacctgcagc tggtgaaaga
    11161 atcatcatgg caagtggttg agcgatcatg ataaatcccg cacttcttgc atccagattt
    11221 tgaatatact gaagataaag actcatgaaa aaccctattg cagaagttgc agcgtaatgt
    11281 atcagagctg caatccctga aaatgtaagg actctgttat gtaaaaagag ttttatatcg
    11341 aagacaggat agtttaccga tttttcaaaa agaatgaaaa aaatgccggc taaaattcct
    11401 gaagaaagac agatccagct gcaaaaagaa ggaagctttg aaaaaccgta catgaaaagg
    11461 gcaagagaga tcccgtataa aattgaacct ttccaatcaa atttttcacc acttgcttct
    11521 gcccagtcgg ttttaatttt tcttgagatt aaaatctggg cgattatacc aatcggaaca
    11581 agaaagacga aaatacttct ccacccgaat tcccttgtta agaagcctcc tactatcggg
    11641 cctgctgaca gaccgaggta tacagctgtg atatttattc ccattgcctt tcccctttcg
    11701 cccggtggaa aaactgatgt tatgattgcc agacttgtac cgaaaaacat tgcaccggag
    11761 agaccctgaa agattctcat aataatcagg gagtggatat ccctgcaaaa gattatgaaa
    11821 aatgttgaaa gagtaaataa aaagatacca gctctgaaaa ttttgattct tccacggata
    11881 tctgcaagct tacccattgg tagcagaaaa atagcggaag taagtataaa actgctgact
    11941 atccatccca attccacagc atctgtattc agatcctttt ctatcgccgg aagagctaga
    12001 tttattgcag aaccaagaaa aggattgaga aatgaagcaa atgttgctac cagaagaacc
    12061 gatttttgaa gtgatttatc tccaggaatg ccttgctctc ttttggtcat ttagaaatct
    12121 ttgttttttg tgtcaataat tattgtaacg gggccatcgt ttataagttc aacatccatc
    12181 attgcaccaa attttcctga agctgttttt ctacccagcc gttcggaaag agctataata
    12241 aatttttcat acaaaggaat tgctttatca ggatgggcag cccttatgta tgaaggtctg
    12301 tttccctttt ttgtcagtgc atgaagagta aactggctga ctaccagtat attaccttct
    12361 gtttctttca ctgaaagatt cattacgcct tcattgtcgt tgaatatacg gaggttaact
    12421 attttattgc tcagccattc aatatcttct tctgtgtctg catcctcaaa gcctgcgagg
    12481 acaagcagac cgggcccgat ctgtgaaatc aactcaccat ccactgtaac tgaagccctt
    12541 ttaactcttt gtattacaac tctcatttat aggcaaattt tatagaacca aatataaaaa
    12601 catgtttaaa aaatgaggat taacttctta tttgcttttt attgtgatga aaatctcttc
    12661 acccggaatc agttttaaag gcatgccttc aataagttcc ctgccggaga attttttcat
    12721 cttattaccg ggcttgaaaa acactttata tactttgtca tattcaggga taaaccactg
    12781 ttgaaactgg ttaattcttg gatagtctac cgggagatgc attttttctt tataccttgg
    12841 gatgccgaat tgcaatttac cttcccatac gttttcacaa ctcgctgcaa ttataagttg
    12901 gccagattca tatacagcgc caattttcag tttttcgtcc caatggtcag ggattattcc
    12961 ctgtgttttc cagaggcaat acataattgt tgttctagca aaattaccgt cgccgtgcca
    13021 gccttctata atgccatcag gtttctggta attccacata atcctgattt cgctgtcaat
    13081 ccattttttc agtttgggca atggaattct gttgtacaga ttaagtgcac cttcgatggc
    13141 atcagcatat ccgtcacatc caccattttc ccagttgaaa ttcctgtaat tatcgtatag
    13201 aatatcaagt gctttaatta cagcttctct gtattctgga acggaatcaa tatccgcaat
    13261 aaaataatac gcattgagag tatatccaaa ggtgtcggca ataccatcag atattatctc
    13321 gcctgtttga gggtttattt cgttgtaaaa taaaccatct tcatttcttc cgacttcaag
    13381 tattctattg agcataatat gcaagttttt tcgccattgt gctctttttt ccggccatac
    13441 atagtaagct gcaaggtagg cttcgcaaag cccagataaa atttcacagc cgtgatccct
    13501 aatgcgaata tggtcagaat gttgtgtgat aagattttca tcactcaggt aatagtctgc
    13561 aatctcggca gcccattcaa ggtatttccg gttaccggta aaccagtaca ttctcgaaag
    13621 agtctgcaat agatccccgt tgacctcaac aactgcattc cttccaaaag aacttccctt
    13681 tagttcttta accactgttg caattttctg gaaatcctcc agtatttcaa gcatcctgtc
    13741 actccatata ctttttccca gccattcggt aataggtatg agtccgtctt tcatgtattc
    13801 agcagatcca aatattattt gtgaagtatc aattgcctcg tttctgaaac ctttcctgga
    13861 aaacgaatat gtatcgggca gttttccgat ccttgacgtt agttttcttt cattttcaag
    13921 catttttact gcagcagtat tgaaaaaatc gggcattagc aacgatgacg taagaaccat
    13981 aaagggataa ttatctgctg ctgcatccca tgcattccag tagtctatgc tttctcttaa
    14041 attgcgtgga attaggccac tttcaggatc agcatgacga ctccatgcat taacataagc
    14101 tatgcaacgg ttaaagcctt catttgaaag aatactattg tctgaagctt ctttgaaaac
    14161 cgaattatca gattttttac aggacaataa agagatagcg ataatcagaa aaacaattaa
    14221 acgtaccatg gttaaatttg atattggaat ttggaaaata tcatgagcaa acttaatgat
    14281 aataataaaa taattttgtg gaatttcaga aagagtgtag acttttccgg tatatttgtc
    14341 caattttttt tgtgatggat tttgttacaa ttcttttaat tgctctcggt ttgtcatttg
    14401 acacatttgc tgcatcagtg gttttcggta taacatgcag caggattgtt cttgctcagg
    14461 caataagggt ggcaattgta atggcattgt ttcagggagg gctaaccgta acgggatatt
    14521 ttgcagggac agtgattagt ggacctttga aattactgga tcactggatt gctttaatac
    14581 ttttacttgt tattggaatt agaatgatta ccggaggtat ccagaagaaa cagcatctta
    14641 aaccaaggga ctacacacga acgcctgaac ttatagccat agcattaagt accagcattg
    14701 atgcagctgc ggtaggtata agttttgctt tactgcaggc aagaatatgg ttctctggag
    14761 ctgtaattgc gattgttaca tttcttgcct caatgacagc t
//
LOCUS       DAOSNB010000025        14298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4271_length_14298_cov_3.583444, whole genome shotgun sequence.
ACCESSION   DAOSNB010000025 DAOSNB010000000
VERSION     DAOSNB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4271_length_14298_cov_3.583444"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="PLG42_02160"
     CDS             <1..1236
                     /locus_tag="PLG42_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30046.1"
                     /translation="PEHPEYIDNIIHEMQKIIDDMKVLYGVDNPLFQKEKKHLIVPLF
                     LKWLYKFIHTMYRIKKLNQPYEDFLLKLSKNESLNDIIGQHFFRNTPAFFALSYFALF
                     NDYMYPYGGMGNFVGKLVEKFKELGGEVQNNTTVTTINPENNSIQTQTGEVYAYENLI
                     WAADLKYFYKNLKTTAEDKKVTRLKNKILSSKGAESVFSVFMAIDEPPEFFAKISGGH
                     IFYTPFKRGLLELKNSDDILNKWTDLTKQDKIDWLKEFIKYNTFEISIPSLRDQTASP
                     EGKTGIIVSYLFYHDITERIAEEGWYDEFKLIMEDEVVSVLSEHFYKGIRDKILFKLS
                     ATPLTIEKRVLSSEGSIVGWSFRDKIPVENHIFYLGKAVQTPFRNIFTTGKWVYSPAG
                     IPTAIMTGRIAAKAILKKR"
     gene            1238..2824
                     /locus_tag="PLG42_02165"
     CDS             1238..2824
                     /locus_tag="PLG42_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU30047.1"
                     /translation="MKQIVVVGGGISGLTCTAYLARAGYKVLLLEKNNEFGGLVSSIE
                     HNGFTFEAGVRALVNAGIILPMLNDLGIQLEMVPNKVSIGIEDKIIHVDSKEDVEKYR
                     EMLIDLYSGSSNEIYNFIRRMQKITGYVDTMYKIDNPVFRDLKKDREFLLKKLLPWLP
                     RFLLTLQKINMLNEPVEQYVARFISDPSVRDIITQHFFKSTPAFFALGYFAIYAEYYY
                     PIGGVGRLAEAVQKKALELKAELKTGKLIREVNAAEQYVIDQNGAKYKYDYLVWAADL
                     PTFYDIVNLNGLPLIIKNKVLNLKSKFRKARSSESVFSLYLEVDLPLEYFGKISHGHF
                     FYTPSKKGLGSIHTSELESLPEDFAYNNKDEILSWLDRFLEHNTFEISIPGLRDKNLV
                     PEGKTGLIISFLTEFWLFEKVKQAGWYEEFKDIIENKIIDILSRTIYNGLKERIEHRF
                     SFTPLSIKKRIASTNGAIVGWSFERDVPAVSNMLKVNQSVLTPLPNTFQTGQWTYSPA
                     GVPMAILTGKLAADKLLKCK"
     gene            2833..3282
                     /locus_tag="PLG42_02170"
     CDS             2833..3282
                     /locus_tag="PLG42_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOU30048.1"
                     /translation="MRIWSIHPKYLDVKGLTALWRETLLAKNVLEGNTKGYKNHPQLI
                     RFKNADDPLKAINLYLEVIYRESLKRGYRFDRNKFTPQKDDGKHLTVTRGQLEYEWQH
                     LLNKLKKRDITRYNRLSGIKEIECHPLFVVTDGDIEDWELVKNRPER"
     gene            3449..4051
                     /locus_tag="PLG42_02175"
     CDS             3449..4051
                     /locus_tag="PLG42_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30049.1"
                     /translation="MTKTGQFVCRNSCRKVVQTVSPVNTKPEEWVVIAACNLKNYNNM
                     TAQEGETLIYKGKKTTMATEPLEQYLLTRPDIKFLSPSTACWRGYYGKWEIQDNKLYL
                     TGLKAYIEGYREVNLDYLFPGQKRVFASWFNGEIRIPMGEMLQYVHMGYCSVFEKDII
                     LTFIDGILTGEMVIDNRKKFKNNQNIDSSTGSNFSDGDKN"
     gene            4252..5058
                     /locus_tag="PLG42_02180"
     CDS             4252..5058
                     /locus_tag="PLG42_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOU30050.1"
                     /translation="MKDYYKILGLSRNATPEEIRQRFRELALENHPDVSENKNASEVF
                     IEIYEAYHILSQPEKKIKYDLLYDKYFRRERVEIPDEEYIKNDIHNVSSSAKERAQQK
                     AKVRYSDFIKDMDCFFIPGLKADGKPYSFSMHKNVGISGGVGPMGSIKSKVINIPIPR
                     SKKAHVLHQIGFSIKVLFFVLAIIAFKLDLLSNYNLVTKLVISTFIIIIGGLITFIFY
                     RLYKVKSKFFFSGKYPLVRKYKLNGYKRGFHPMISTTPVGLIAYLLRLIF"
     gene            complement(5135..5532)
                     /gene="ssrA"
                     /locus_tag="PLG42_02185"
     tmRNA           complement(5135..5532)
                     /gene="ssrA"
                     /locus_tag="PLG42_02185"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5779..8058
                     /locus_tag="PLG42_02190"
     CDS             5779..8058
                     /locus_tag="PLG42_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU30051.1"
                     /translation="MVFLSGCVKKWTEEPKEGYVLVHNKKGPVLGYSKSSGVSIITSG
                     GYAFKDLNRNGKLDAYEDWRLSSDERAKDLASKLSVEQIAGLMLYSSHQSIPAGEGGR
                     MGATYNGQPFSKSGAKASDLTDQQKKFLEDDNVRHVLITRVQSPEVAAQWNNNAQAFV
                     EGLGMGIPINTSSDPRHGTEATAEFNLGAGGNISMWPNTLGLAATFDPNLMLQFGKIA
                     STEYRALGITTALSPQIDLATEPRWGRFNGTMGEDPDLATDMARAYVDGFQTSDSAYE
                     ISGGWGYRSVNAMVKHWPSGGPEEGGRDGHFGYGAYAVYPGNNLKDHLKPFVEGAFKL
                     NGPTGMASAVMPYYTISYNIDTVYHENVGNSYSKFVITDLLRKKYNFDGVVCSDWGIT
                     GEVEGVYQFQGKPWGVEKLTVSERHYKAIMAGIDQFGGNNDKGPVLDAYNMGVKEHGE
                     EFMRKRFEESAVRLLKNIFRVGLFENPYLDPEETKKIVGNPEFMKAGYEAQLKSIVML
                     KNKNNVLPLKEKVKVYIPKRKVSGGGGFFGGPAQERIVDGLAPDIAGRYFELTDNPAE
                     ADAAIVVIDSPRGGSGFNPDELAKGGTGYVPITLQYEKYTAVHARPVSIAGGSPFENF
                     TNRTYRGKTVIATNSGDLKLVRDTRKAIGNKPLILVVNTSNPFVLAEMENICDAILLH
                     FAVQNQAILDIISGKAEPSALLPFNMPADMKTVEEQFEDVPHDMKCYTDAEGNTYKFG
                     FGLNWKGVINDERTAKYRK"
     gene            8128..9816
                     /locus_tag="PLG42_02195"
     CDS             8128..9816
                     /locus_tag="PLG42_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOU30052.1"
                     /translation="MKTTRRNFLIITGAGGAGMAIGAPLLASGCKPAKDVPVSYNEQV
                     LFIGDNIAVTETKYGKVKGYILRGIYYFLGIPYGADTSGPNRFMPPQKPKPWDDVFPA
                     VWWGNSAPQNMERRYANRYQSFVDHWNYDDVSEDCLRLNVFTPAINDGKKRPVLVWLH
                     GGGFTTGNGIEQDGYNGENFCRKGDVVFVSINHRLGPMGFSNFAAVGGEKYAASGNVG
                     MLDIVAALEWVRDNIENFGGDPGNVTIMGQSGGGAKVTTLTAMPAAKGLFHKAVVLSG
                     AGVMSGDKDYSEKLGEYILKEAGLKPEEIGKLHEMPWQEYYQLANRAATKLAAESAPA
                     SGGMRRGFNPCVDGVILPQHPYHPEATPLASDIPMIICSTLNEISPSRDDASLENITL
                     DEVVERVKQRAGFSSGYGEKAKEVVEAYAKAFPDKKPVEIWSMVMSTRQSVVTLANAK
                     SKQPAPVYVAWFCWQPPLFDGRLRAFHCSDICFWFYNTDLMLTHTGGGPRPRKLSEKM
                     SGALLQFMRTGNPDGGGLPHWPRYTPENGEVMVLDDVSEVKNDPDREARKALPV"
     gene            9945..11162
                     /locus_tag="PLG42_02200"
     CDS             9945..11162
                     /locus_tag="PLG42_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU30053.1"
                     /translation="MKNFKPALVTFFTIFIAVLNSGAQDRPAVPAARPQMRAPARIIS
                     PEIHPDNKVTFRLYARNATTVVVTGEWQSGFGASEAMVKNDTGLFSLTVGPLKPELYG
                     YSFIVDGVTAIDPNNVLVRRDGARYQNFFIVPGPESDLYIHKHDVPHGNVSKVWYKSS
                     VLGMERRMYVYTPAGYETGKGKYPVFYLLHGAGGDEDAWTNMGRAAQILDNLIAQGKA
                     KPMIVVMTNGNANQAGAQNEVPPVPAQDMQSLEAYQRYAGKFEEHLVKDVKPYIEKNY
                     RVLTGRDNTAIAGLSMGGAHTQTITFDNPGMFGYIGVFSMGLMNLRQRDQNVEELEKE
                     RESKLIALQKSGYKLYWIGCGKDDFLYQSVVNFRNFLDKHNFKYFYRESTGGHTWANW
                     RIYLSEFAPLLFK"
     gene            11405..13813
                     /locus_tag="PLG42_02205"
     CDS             11405..13813
                     /locus_tag="PLG42_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOU30054.1"
                     /translation="MKRKLIVLLVAVVIFFDGFAQQLPQLGKDPVKKVVSAMTLEEKV
                     SIVVGAGMRMPGAPPTGGQGQQQRPQQQQQQAPVIGETQDIVPGAAGSTHAVPRLGIP
                     SIVVADGPAGLRISPTRPNDPNTYYCTAFPVATLLASTWDVDLVNKVGQAMGKEVLEY
                     GVDIILGPGMNIHRNPLCGRNFEYYSEDPLVTGKMAAAIIKGIQSNGVGTSAKHYAAN
                     NAETNRNSLNTIVSQRALREIYLKGFGIAVNEAQPWTIMSSYNLINGTYAPENHDLIT
                     KVLRNDWGFRGFVMTDWFGGRDAVAMMLAGNDLLMPGNPNQSQAILEAVKSGKLSEQV
                     LDRNVERILNIILQTPRFKGYKFSNKPDLKANALVTRQAATEGMVLLKNDGGALPFSS
                     EIKKLAAFGNTSYEIITGGTGSGDVNEAYSVSLVEGLRNAGYTLNDNLMNMYDAYLKA
                     AKEGRPRSRGFMMGTIPVPELAVNADLVNSMVNVTDAALITIGRNSGEGRDRQAEKGD
                     FYLTDAEVDLIKTVSEIYHSKNKKVVVILNVGGVIETASWKNYPDAILLAWQGGQETG
                     NSIADILTGKVNPSGKLASTFPVKYEDVPSAKNFPGKVIEQTQPQQQQGEQDMISAFM
                     RPRASEVTYEEGIYVGYRYYNTFKVPVSYEFGYGLSYTTFEYSNLKLSSNTFNKSLTV
                     TVNVKNTGNVAGKEVVQLYLSAPAVRLHKPESELKGFAKTRLLQPGESQTLTFVLDPM
                     SLASFDEATSSWIADAGKYTVKVGASSSDIRLTGQFTLPKNITVKKESMALAPKVKIN
                     ELKP"
     gene            13810..>14298
                     /locus_tag="PLG42_02210"
     CDS             13810..>14298
                     /locus_tag="PLG42_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU30055.1"
                     /translation="MKRANFIPFVILIFLMPVALITAGIGNKPGTERINQTEPWFSVK
                     EYSKGVWIIGDHGADNMYLVVGKDSALLVDTGLGVADLAATVKKITDKPLIVVNTHGH
                     PDHSGANYQFEKVFMHPADILAAKQFNQPEARARSAGNMTQGSEPREEEKYKGTPYDT
                     KYI"
BASE COUNT         4485 a         2897 c         3226 g         3690 t
ORIGIN      
        1 cctgagcatc ctgaatacat cgacaatatc atccatgaaa tgcagaaaat cattgatgat
       61 atgaaagtgc tctatggtgt cgataatcct ttatttcaga aagagaaaaa gcacctgatt
      121 gttccgcttt ttctcaagtg gctctataag tttatccata caatgtacag gattaaaaaa
      181 ctgaatcagc cctatgagga ttttctgctt aaattatcga aaaacgaatc gctgaatgat
      241 attattggtc agcacttttt caggaatact cctgcttttt ttgcattgag ctattttgca
      301 ttgttcaacg actatatgta tccctatggt ggaatgggaa attttgtggg taaattggtt
      361 gagaaattca aagagttggg cggtgaagtg caaaacaaca ctactgttac aaccataaac
      421 cccgagaata atagtattca aactcaaacc ggtgaagttt atgcttatga gaaccttatt
      481 tgggctgccg atttgaaata tttctataaa aacctgaaga cgactgctga ggacaaaaaa
      541 gttaccagac taaagaataa aatattatca tcaaaaggtg ccgaatctgt attttcagta
      601 tttatggcaa tagatgaacc gcctgaattt tttgcaaaga tttcaggcgg ccatattttc
      661 tatactccct ttaaacgcgg attattggaa ctgaaaaact ccgatgacat tttgaataaa
      721 tggaccgatt taactaaaca ggataaaatt gattggctta aagaatttat aaaatacaac
      781 acgtttgaga tttcaattcc ttcgctccgt gaccagaccg cttcgcctga aggcaaaacc
      841 ggaataattg ttagctacct cttttatcat gatataacag aaagaattgc tgaagaaggg
      901 tggtacgatg agtttaaact gataatggaa gatgaagttg tctcagtttt atccgaacat
      961 ttttataaag gaataagaga taagattctt ttcaaacttt cagctacacc tttgacaatc
     1021 gaaaaacgag tattgagttc agaaggctcg attgtaggat ggtcgtttag agacaaaata
     1081 cctgttgaga accacatctt ttatcttggt aaggcagtgc agactccttt caggaacatt
     1141 ttcaccacgg gcaaatgggt gtatagtcct gcaggcattc caacggccat catgaccggg
     1201 cggattgcag caaaagctat cctgaaaaaa aggtaaaatg aaacaaatag ttgttgttgg
     1261 aggtggtata tcgggtttaa cctgtacagc atatcttgct cgtgcaggat ataaggtatt
     1321 gcttcttgaa aagaataatg aattcggcgg actggtcagc agtatcgaac acaatggctt
     1381 taccttcgaa gcaggtgtca gggctcttgt aaatgccgga atcattctgc ccatgctcaa
     1441 cgatctgggg attcagcttg agatggttcc caataaagtg tcaattggca ttgaagataa
     1501 aattattcat gttgatagta aggaagatgt agaaaaatat cgagaaatgc tgattgacct
     1561 gtattccggc agcagcaatg aaatttataa tttcatccgc cggatgcaaa agattaccgg
     1621 atatgtcgat accatgtata agattgataa tccggttttc agagatttga aaaaggatcg
     1681 ggagttttta ttgaaaaaat tactgccctg gctgccacgg tttctgctta cattacagaa
     1741 aatcaacatg ttgaatgagc ctgttgagca atatgttgca agatttatca gcgatccatc
     1801 tgttcgcgat atcatcacgc aacacttttt taaatcgact ccggcctttt ttgccctggg
     1861 ctatttcgcc atttatgccg agtattatta tcccatagga ggagtgggca ggctggcaga
     1921 agctgttcag aaaaaggctc tggagctaaa agcagaactt aaaacaggca aactgataag
     1981 ggaagtaaac gctgcagaac aatatgttat tgaccagaac ggagcgaaat acaaatacga
     2041 ctatcttgta tgggctgccg atttgccaac cttttatgat attgtcaatc tcaacgggtt
     2101 gcccttaatt attaaaaaca aggttcttaa cctgaaatca aaattcagaa aagccagaag
     2161 tagcgaatcg gtattctcat tatacctcga agttgattta ccactcgaat atttcggaaa
     2221 aatatcacat ggtcattttt tctatactcc atcgaaaaaa ggattgggtt ctattcatac
     2281 ttcagaactg gaatctttac ctgaagattt tgcatataat aacaaggatg aaatactttc
     2341 ctggctggat cgctttcttg aacacaacac ttttgaaatt tctatcccgg ggttaaggga
     2401 taaaaatctt gtacctgagg gcaaaacagg cctcatcatc agcttcctga ctgagttctg
     2461 gttgtttgaa aaagtcaaac aggcaggatg gtacgaggag tttaaagata ttatagaaaa
     2521 caaaataatt gacattctat cccggacaat ctataatggt ctgaaagaaa gaattgaaca
     2581 cagattttca ttcactcctt taagtattaa gaaaagaatt gcttcaacaa atggggcaat
     2641 tgtcggttgg tcgtttgaaa gggatgttcc tgctgttagt aatatgctta aagtgaacca
     2701 atcggtttta acgccattgc ccaatacctt tcagacaggg caatggacat acagcccggc
     2761 gggtgttcca atggcaatat taacaggtaa actggcagca gacaaactgt taaaatgtaa
     2821 gtgatttccg caatgagaat atggtcaata catccgaaat atttagatgt taaaggtctg
     2881 acagcactct ggcgcgaaac attgctggca aaaaatgttc tcgaaggaaa cacaaaagga
     2941 tataaaaatc acccgcaatt gattcgcttt aaaaatgctg atgatccatt gaaagctata
     3001 aacctgtacc ttgaggttat ttatcgggaa tctcttaaaa ggggatatcg gtttgacagg
     3061 aataaattta cacctcagaa agatgacggg aaacacctga ctgtaacaag gggacaactg
     3121 gaatacgagt ggcagcattt gctgaataaa ctgaagaaaa gagatataac cagatataac
     3181 agactatcag ggattaagga aatcgagtgc catcctcttt ttgtagttac agacggagat
     3241 attgaagact gggaactggt aaaaaacaga cctgagcgat aaccggatat tgaataaagt
     3301 tggcattaac aaatgataga attaattttc tcgaaacttt gctctttcat tttatcctga
     3361 gtacatttga taaataattt acacgtagta aggacaccaa actcacattg aggataagtt
     3421 aagattgttg aatgcaggat aagagcctgt gacaaaaaca ggacagttcg tatgcaggaa
     3481 ttcctgccgt aaggtagtgc agacagtaag tccggttaat accaaaccgg aggaatgggt
     3541 agtaattgca gcgtgcaacc tgaaaaatta taataacatg acggcacagg aaggagaaac
     3601 ccttatctac aaaggtaaaa aaacaaccat ggccacagag ccgctggagc agtatctgct
     3661 tacgcgtcct gatattaaat tcttatcgcc ttcaacagct tgctggcgtg gatactacgg
     3721 aaaatgggag atccaggaca ataaactcta ccttactgga ctcaaagctt atatagaagg
     3781 gtacagggag gtgaacctgg attacctgtt tcccggtcag aagagagtgt tcgccagctg
     3841 gttcaacggt gaaataagaa tccccatggg cgaaatgctt cagtatgtac atatgggata
     3901 ctgttcagtg tttgaaaaag acattatact gaccttcatc gacggtattc tgaccggaga
     3961 aatggtgata gacaacagaa aaaaatttaa aaacaatcag aatattgata gttccactgg
     4021 cagcaacttc tctgatggtg ataaaaatta aaaactcttg tcttgataaa aataaactta
     4081 tctttacttc attaagtgga tatattttga ctttagacgg gtgtaaatgt agggctcaga
     4141 aataacaacc caaaaataaa tcgcctgcag cggctgtgaa ctattaaaac ggtgtaaatt
     4201 actgaatccc ctctccagag aatagccgct gttgacaaaa tttagtgact aatgaaagat
     4261 tactacaaaa ttctcggttt atcaagaaat gcaacccctg aggaaatcag acaaagattc
     4321 agagaactcg cccttgaaaa tcatccggac gtcagtgaaa ataaaaatgc cagtgaagtt
     4381 tttatcgaga tttatgaggc gtatcacatt ttaagccagc ccgagaaaaa aatcaaatac
     4441 gatttgcttt acgacaaata cttcaggaga gaaagggttg aaattcctga cgaagagtat
     4501 ataaaaaatg atattcataa tgtttcttct tcagcaaaag agagggctca acaaaaagca
     4561 aaagtaagat acagtgattt cattaaagac atggattgct tttttatacc cgggttaaaa
     4621 gctgatggga aaccatattc attcagtatg cataaaaatg ttggaatttc aggtggtgtc
     4681 ggacctatgg gaagcataaa atcaaaagtc ataaatattc caatcccgag aagcaaaaaa
     4741 gctcatgtgt tgcatcaaat cggattttcc ataaaggtac tattctttgt tctggcaata
     4801 attgcgttta aacttgattt attatcaaat tataacctgg ttacaaagct ggttatttcg
     4861 acatttatta taattatcgg cggacttatc acattcatat tttaccgtct gtataaagtc
     4921 aaatcgaaat tcttcttttc cggaaaatat cctcttgtta gaaagtataa acttaacggg
     4981 tataaaagag gatttcatcc gatgatatcg accacacctg ttgggctgat tgcatatctt
     5041 ttaagactaa tattttaaaa atacaataaa aatttgtctt tatcagctta taaagaagaa
     5101 ccactctctt acgggagtgg cagttcttgt tttatcgtgg agctggcggg agtcgaaccc
     5161 gcgtccagtc atggagacca tatgctttct acatgcttag tcctgctcca ttgtcgggac
     5221 cacctgggtg caggacaacc ctgatgatcc gtagttccgt ttctgtctcg ccctctggcc
     5281 ggaacaacct tcgggccagc cccgatttcc acgcgcctcc gtatcgggtt ggctcgcggc
     5341 tacgccgccc gggagacgtc ccgctcccgg cactgtgccg ggataaagct taaactactt
     5401 cgttcgttta agcagcgaga gcgtaattgt attcgccgtt tattgagtcc gtgacaccgt
     5461 ttatagcgac gtgtcaccac acgctgcatg cttacatacc acctctcatg ctgtcaaaac
     5521 caggtcagcc ccggtttacg gacaacaaaa ttacaaaaat tattccagaa tctgagttaa
     5581 aagtattatc cgcctgctcg aaatcttttt tttgcatttt aacaatcttt aataattctg
     5641 aatgtgtcaa aatattaaag cattacttaa tttagagcat aatttacaag ttacatgaaa
     5701 cattaataat caaatagaag aagtaaaatg aaaaaattat tctccccctt tattattatt
     5761 attattactt tccccggcat ggtatttctt tcgggatgtg taaaaaaatg gactgaagag
     5821 ccaaaagaag gttatgtact ggttcataac aaaaagggtc cggtgctggg ttattcaaaa
     5881 tcgtcaggtg taagtatcat aacatctggt ggatatgctt tcaaggatct caaccgtaac
     5941 ggaaaactcg atgcatatga agactggcgc ctttcgtcag acgaaagggc aaaagacctt
     6001 gcctcgaagc tgtcggttga acagatcgcc ggactgatgc tctacagcag ccatcagtct
     6061 atccctgctg gcgaaggtgg cagaatggga gccacttaca acggccagcc gttcagtaaa
     6121 agcggagcaa aagcttccga ccttaccgac cagcagaaga aatttcttga ggatgataat
     6181 gtaaggcatg tgttgattac aagagtacag agccctgaag tcgcagcaca atggaataac
     6241 aatgcccagg catttgttga aggtcttgga atgggtattc caataaatac cagttccgat
     6301 ccgcgccacg gcacagaggc aacggcagaa ttcaacctgg gtgccggagg caatatttcc
     6361 atgtggccaa atactctggg ccttgctgct acatttgatc ccaacctgat gctgcaattc
     6421 gggaaaattg cctcaacaga ataccgtgca cttggaataa ccacagccct ctcaccccag
     6481 attgaccttg ctacagaacc ccgctggggc cgcttcaacg gaacaatggg cgaagacccc
     6541 gaccttgcca ctgatatggc aagagcatat gtcgacggct tccagacttc cgactcggcc
     6601 tacgagatct caggaggctg gggttaccgc tcggttaatg ccatggtcaa acactggcct
     6661 tctggcggac ccgaagaagg aggaagagac ggccatttcg gatatggcgc atatgcagtc
     6721 taccccggta ataaccttaa agatcacctg aaaccttttg ttgaaggagc attcaaactt
     6781 aacggaccca ctggtatggc ttcagcagtt atgccttatt atacaatctc atataatatc
     6841 gacaccgttt accatgagaa tgtcggaaac tcttacagca aattcgtaat aacagacctg
     6901 ctaagaaaaa aatataattt cgacggcgtt gtttgcagcg actggggaat cacaggcgaa
     6961 gttgagggag tctaccagtt tcagggtaaa ccatggggcg tcgaaaaact gacagtaagc
     7021 gaaagacatt acaaagctat catggcaggt atagaccagt tcggcggtaa caatgataaa
     7081 ggtcctgtat tagacgccta taatatgggt gtaaaagagc atggtgagga gtttatgcgt
     7141 aaaagattcg aggagtctgc cgtaagactg ctcaaaaata ttttcagggt aggattgttt
     7201 gagaacccgt atctggatcc ggaagaaact aaaaagatag tcggaaatcc ggagtttatg
     7261 aaagcgggtt atgaggctca gcttaaatca attgtgatgc tgaaaaataa aaataacgtt
     7321 cttcctttaa aagaaaaggt caaagtttac atacccaaac gcaaggtttc gggagggggc
     7381 ggcttcttcg gaggacctgc tcaggaaaga atcgttgacg gattagcccc tgatatagca
     7441 ggccgttatt ttgaacttac tgataatcca gctgaggccg atgctgcaat agtagtgatt
     7501 gacagtccaa gggggggatc gggtttcaac cccgatgaac ttgcaaaagg aggtacagga
     7561 tatgtaccga taacacttca gtatgagaaa tatactgcag ttcacgcacg tccggtaagt
     7621 atagcaggtg gaagtccgtt tgaaaacttc accaaccgta cctacagggg gaagacagtt
     7681 atcgcaacta actcgggcga cctgaaactg gtaagagaca cacgtaaagc cattggaaac
     7741 aaaccattaa tccttgtagt taatacctcc aatccgtttg tacttgctga aatggagaat
     7801 atttgtgatg caatcctgct tcattttgca gtccagaatc aggcaattct cgatattatt
     7861 tccggtaaag ccgaaccatc agctcttttg ccttttaata tgccggcaga tatgaagacg
     7921 gttgaagaac agtttgaaga tgtacctcac gacatgaaat gttataccga tgctgaaggc
     7981 aatacttaca aatttggttt tggattgaac tggaaaggtg taataaacga cgaaagaaca
     8041 gcaaaataca ggaagtgaaa tacgatgtag taatttctgt tcaagagtta ttacaatata
     8101 gtttcatgaa ataaaaaata ttatagcatg aagacgacaa ggcgaaattt tctcataatc
     8161 accggtgcag gaggtgctgg catggcaatt ggagcccctc ttcttgcatc aggatgcaaa
     8221 cccgcaaagg atgtgccagt cagctataat gaacaggttc tgtttattgg cgataacata
     8281 gctgttaccg aaacaaaata cggtaaagta aaaggctata tactgagagg tatctattat
     8341 ttcctgggta tcccttatgg agccgatact tcaggtccca acaggtttat gccaccgcag
     8401 aaacctaagc catgggacga tgtctttcct gcagtgtggt ggggaaattc tgcaccgcaa
     8461 aacatggaaa ggagatatgc caaccgttac cagtcattcg tcgaccactg gaactacgat
     8521 gatgtgagcg aggattgcct gagactgaat gttttcaccc ctgcaatcaa cgacggaaag
     8581 aaacgtcctg tactggtatg gctccacgga ggaggcttta ccactggcaa cggcatagaa
     8641 caggatggct ataatggcga aaacttttgc cggaaaggtg atgtggtctt tgtttcaata
     8701 aatcacaggc ttggacctat gggcttttca aattttgctg cagtaggtgg cgaaaagtat
     8761 gctgcttctg gtaatgtcgg aatgcttgat atagttgctg cacttgaatg ggtacgcgat
     8821 aatatcgaaa actttggagg cgaccctggt aatgtaacaa ttatgggaca atcgggaggt
     8881 ggtgccaagg taactacact cacagccatg ccagcagcaa aaggtctttt ccataaagcc
     8941 gttgtactga gcggtgcagg tgtgatgtcg ggcgataaag attattccga aaaacttggc
     9001 gaatacatac tgaaggaagc agggttaaaa cctgaagaaa tcggcaaact gcatgagatg
     9061 ccatggcagg aatattatca gcttgcaaat cgtgctgcca caaaactggc agccgaatcg
     9121 gctcctgcat ccggaggtat gagaagaggg tttaatccct gcgtcgacgg agtcattcta
     9181 ccccagcatc cataccaccc cgaagctaca ccacttgcat cggacatacc tatgattatt
     9241 tgttctactc ttaacgagat atctccaagc cgcgatgatg cctcgcttga gaatataaca
     9301 ctcgatgagg tggttgaaag ggtgaagcag agggcaggct tcagctcggg ctacggtgag
     9361 aaggcaaaag aggttgttga agcctatgca aaggcttttc ccgataagaa gcccgtcgag
     9421 atatggtcaa tggtgatgag tacacgccag agtgttgtta cactggcaaa tgccaaatcg
     9481 aagcagcccg ctccggttta tgtggcatgg ttctgctggc agccacccct cttcgacgga
     9541 cgtttacggg cattccactg ctccgacata tgcttctggt tctataacac cgacctgatg
     9601 ctgacacata caggcggagg accacgtccc agaaagctct cagagaagat gtcaggagca
     9661 ctcctgcagt ttatgcgcac aggcaatccc gatggcggcg gactaccaca ctggccgaga
     9721 tacaccccgg aaaatggcga ggtaatggta ctcgacgatg ttagcgaggt gaaaaacgat
     9781 cccgaccgcg aagccagaaa ggctttgcca gtttaacacc agacatgtct taaattgaaa
     9841 cttattaaat ttaatcatca cctttttttc cctacgtaat gttgggggca taacaaagaa
     9901 ttaataaaaa tctatttatc aataatttat aaaatagttt gcatatgaaa aactttaaac
     9961 cggcactcgt aacgtttttt acaattttta ttgcagtatt gaacagcggg gcacaggatc
    10021 gtccagctgt accagctgca aggccacaga tgcgggctcc cgcaagaatc atatcacctg
    10081 aaattcaccc tgacaataag gtgactttca gattgtatgc aagaaatgcc acaacggtgg
    10141 ttgtcactgg tgaatggcag agcggattcg gtgcatcaga agctatggtt aaaaatgata
    10201 caggtctttt ttcactgaca gttggtccgc taaagcctga actatatggt tattctttca
    10261 tagttgacgg tgtaacggca attgacccga ataatgttct tgtaaggcga gatggagcac
    10321 gttaccagaa cttctttatc gtaccgggtc cggaatcgga tctctacatt cataaacatg
    10381 atgttcccca cggaaatgtg tcgaaggtgt ggtacaaatc gtctgtcctt ggtatggagc
    10441 gcagaatgta tgtttatact ccagctggtt atgagacagg aaagggaaaa tacccggtct
    10501 tttatctcct gcatggtgcc ggaggtgatg aggatgcatg gacaaacatg ggccgcgctg
    10561 cacagatact cgataacctt atcgcacagg gcaaggcaaa acctatgatt gtggtcatga
    10621 caaacggtaa tgccaaccag gctggcgctc agaacgaggt gccacctgta ccggcacagg
    10681 acatgcagag cctcgaagca tatcagagat atgccggaaa gtttgaagaa catctggtaa
    10741 aggatgtaaa accatacatt gaaaagaatt acagagtact tacaggaaga gacaatactg
    10801 caattgccgg actttcaatg ggaggtgccc atacccagac tatcacattc gataatcccg
    10861 gtatgttcgg ttatataggc gttttcagca tgggactaat gaacctgaga cagagagacc
    10921 agaatgttga agagctcgag aaagaaaggg aaagcaaact gattgccctt caaaagagcg
    10981 gctacaaact ctactggatt ggatgcggga aagatgattt tctgtatcaa agcgtggtaa
    11041 acttccgtaa tttcctggat aaacataatt tcaaatattt ttaccgcgaa agcaccggag
    11101 gccatacatg ggcaaactgg agaatatacc tttcagaatt tgcaccactg ctgttcaaat
    11161 aagtccaccg gactttatat aatttttgcc cttcctggca acactaaaga aaaggagcct
    11221 gtaacccaac cagataattc tcccatgcta cggggataga gggtattgaa aaagtgaggt
    11281 cgaaaacagg agaggtaata agtttaaaaa attgggcaag cggtatttaa aagatgctta
    11341 aatttgcaat acaagtaatg ttttgataat taataacata taaaatatta aaaatcacat
    11401 tgttatgaaa aggaaactta ttgtattatt agttgcagtt gtaatatttt ttgatggatt
    11461 tgcacagcag cttccacagc ttggtaaaga ccctgtgaag aaggtggttt ctgccatgac
    11521 tcttgaagaa aaggtttcaa ttgttgttgg agcaggcatg aggatgccgg gtgctccccc
    11581 aacgggagga caggggcaac agcaacggcc acagcagcaa cagcagcaag ctccggtgat
    11641 aggtgaaaca caggacattg tcccgggtgc tgcgggttct acacatgcag ttcccaggct
    11701 tggaattcct tcaatagtgg ttgctgacgg cccggccgga ctgagaatca gccctacccg
    11761 tccaaacgac cccaatacct attattgcac agctttcccc gtagcaacac ttctggcatc
    11821 aacatgggat gtcgacctcg ttaacaaagt gggtcaggca atgggaaagg aagtgcttga
    11881 atatggcgtg gatattatcc ttggcccggg aatgaatatc cacagaaacc cactgtgcgg
    11941 aagaaacttt gaatattact ccgaagaccc gcttgtgact ggtaaaatgg cagcagcaat
    12001 tatcaaaggc attcagtcaa atggtgttgg tacctctgca aaacactatg ctgctaataa
    12061 cgctgagacg aatcgaaatt ctctcaatac aattgtaagc cagcgggcat tacgcgaaat
    12121 atatcttaaa ggtttcggca ttgccgtcaa tgaagctcag ccatggacca taatgtcttc
    12181 atacaacctt atcaatggaa cttatgcacc ggaaaatcac gaccttataa caaaggttct
    12241 caggaatgac tggggtttca gaggctttgt aatgaccgac tggtttggcg gcagagatgc
    12301 tgttgccatg atgttagccg gtaacgacct tctgatgccc gggaacccaa atcagtcaca
    12361 ggctatcctc gaagcggtta aatcaggtaa actcagcgaa caggtgctcg acagaaatgt
    12421 tgaaaggatt ctcaatatta tacttcagac gccaagattt aagggctaca aattttccaa
    12481 caaacccgat ctcaaagcca acgcactggt cactcgccag gctgcaacgg agggaatggt
    12541 ccttctgaaa aatgacggtg gcgcccttcc attttcatct gagatcaaaa aacttgcagc
    12601 cttcggaaat acttcatatg aaattattac cggcggaaca ggaagcggcg atgtgaatga
    12661 agcatacagt gtttctcttg tagaaggact gcgtaatgca ggttacaccc tgaatgacaa
    12721 ccttatgaac atgtacgatg cataccttaa ggctgctaaa gaaggcagac cgagaagccg
    12781 cgggtttatg atgggaacaa tccctgttcc cgaactggct gtcaatgcag atcttgttaa
    12841 cagtatggta aacgtaaccg atgctgccct gataacgatt ggaagaaact caggtgaagg
    12901 tcgtgaccgc caggccgaaa agggcgattt ctatctcacc gatgctgagg ttgaccttat
    12961 aaaaaccgtt tcggagattt accactctaa aaacaaaaag gttgtcgtta ttctcaatgt
    13021 gggaggcgta atagaaacag ccagctggaa aaactatcct gatgctatac tgctggcatg
    13081 gcagggcggt caggaaacag gtaactccat agccgatatt ctcactggaa aagtcaatcc
    13141 ttcaggaaag cttgcctcta cattcccggt aaaatacgaa gatgtgcctt cagcaaagaa
    13201 ctttccgggt aaagtcattg aacagactca gcctcagcag caacagggag aacaggacat
    13261 gatctcagca tttatgcgtc cgcgtgcatc agaagttact tacgaggaag gtatctatgt
    13321 gggatacaga tactataaca ccttcaaagt tcctgtctct tatgagtttg gctacggact
    13381 ctcctataca acatttgaat actcaaacct taagctgagt tcaaacacat ttaacaaaag
    13441 ccttactgta acggtgaatg tcaagaacac tggtaacgtt gctggaaaag aggttgttca
    13501 gctctatctg tcggcaccag ccgtaaggct gcataaacct gaaagtgaac tcaaaggatt
    13561 tgcaaagaca cgcttgcttc agccgggtga atcacagaca cttacatttg ttcttgaccc
    13621 gatgtcgctg gcatcattcg atgaagctac atcttcatgg attgctgatg caggaaaata
    13681 tacagttaag gttggtgcct cttcgtccga cataaggctc accggacagt tcactcttcc
    13741 aaagaacatt actgtgaaga aggaaagcat ggcacttgca ccaaaggtga aaataaatga
    13801 actgaagcca tgaaaagggc aaatttcata ccttttgtta tactgatttt ccttatgccg
    13861 gtggcattaa ttaccgccgg catcgggaat aaacccggaa cagaacgtat caaccagaca
    13921 gaaccgtggt tttcagtaaa ggaatacagc aaaggagtct ggataattgg cgaccatggt
    13981 gccgataata tgtaccttgt tgttggaaaa gacagtgccc tgctggtcga tacaggtctg
    14041 ggtgttgctg accttgctgc aacggtgaaa aagattactg acaaaccgct gattgtagta
    14101 aatacccacg gccatcccga ccattcaggt gcaaactacc agtttgaaaa ggtctttatg
    14161 catccagccg atattctggc agcaaagcag tttaaccagc ctgaggcaag agcacgttca
    14221 gcaggaaaca tgacccaggg gagcgagcca cgcgaagagg agaaatacaa agggacccct
    14281 tacgacacaa aatacatt
//
LOCUS       DAOSNB010000026        14281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4284_length_14281_cov_4.724518, whole genome shotgun sequence.
ACCESSION   DAOSNB010000026 DAOSNB010000000
VERSION     DAOSNB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4284_length_14281_cov_4.724518"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /gene="ribH"
                     /locus_tag="PLG42_02215"
     CDS             complement(<1..421)
                     /gene="ribH"
                     /locus_tag="PLG42_02215"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOU30056.1"
                     /translation="MATANLSDYDPSKVPDASEMRFGIVVSDWNQEITFSLLDGCVKT
                     LKKHGVTDEDIVIKHVPGSFELTLGAQLMAENEDVDAIICLGCVIQGETPHFTYICQS
                     VSMGITQLNIDYNIPVIFGVLTTNNIEQARERSGGKHG"
     gene            complement(509..1195)
                     /locus_tag="PLG42_02220"
     CDS             complement(509..1195)
                     /locus_tag="PLG42_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU30057.1"
                     /translation="MAKKPKTESPQSIKSVEETLTKTEQFLEENYKVILITLGILVVL
                     VGLFYLSRVYLNKRNQEAQSQMYQAERWLEIDSLNLALNGDGNYLGFLEIAKTYRLTK
                     AGNLAKYCAGICYLHLGKYEEALEYLSKYSIKDNVIGSLAIGAMGDALVELGRLDEGI
                     NRYIEAAEKANNSFNTPLYLMKAGQILEMQQKYAEALKLYERIKNEYPESTEGATIDK
                     YIARVKLLMN"
     gene            1400..2539
                     /locus_tag="PLG42_02225"
     CDS             1400..2539
                     /locus_tag="PLG42_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOU30058.1"
                     /translation="MYIKQLSLTNFKNYDSAFLKFSPGINCLVGNNGVGKTNILDAIH
                     YLSLTKSFYNIVDSANIKHSQEYFIIEGVVTVNGNEDILFCSFHRQKMKIFKRNGKEY
                     NKLNEHVGKFPVVMVSPADSILITGGSEERRKFLNKIISQYDAIYLDAILNYNKALQQ
                     RNRLLKDYAGKISVDREILEVWDAQLIRYGEYIYRERAGLVKELIPVFNEYYSFISGG
                     NETVNLDYRSQLSESDFSDLLEKSLNKDLVLEYTTAGIHKDDLIFEMGGYPVKSTGSQ
                     GQQKSYLVALKLAKFDYVRRRVGFAPVMLLDDLFDKFDIERVEKIIKLVGNHRFGQIF
                     ITDTDEERMKSILSRLNVDHRLFRIEKNGNIEVISDFNNYSNETQ"
     gene            2526..2816
                     /locus_tag="PLG42_02230"
     CDS             2526..2816
                     /locus_tag="PLG42_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOU30059.1"
                     /translation="MRRSKTISLAEAINDYINEMKIGGKLQEINIIDSWEKIVGKAIS
                     SRTSRVTIKNGVLTVYLKSSVVKSELMMIREAIKEKINREVGSEFIKEIVLR"
     gene            complement(2819..4567)
                     /locus_tag="PLG42_02235"
     CDS             complement(2819..4567)
                     /locus_tag="PLG42_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30060.1"
                     /translation="MSQSYPVDFSDKRNLEKYSSAFTLSDMEVFVFPELLYPLVLANI
                     MSPVIWKWREDSWFDGIYNKKFLYKVNRIKQYIIQNYIFNLDLATWGLTTKTKEIERF
                     RDFIDMNTLRQSNALFGYEGDKYYFDIDIRKHFGLDKYDSDVIPYWKTETVEAMEAFR
                     FKEGFTTGAGECVSLSALYAAAMFIIGRIPLEKIFLMATPLHSQNFITESEGILTNNR
                     RILTKNMWFNGTSLSAKARRALENEKVTIVSNISGYIHILYDVATINPEDYRTFSEKL
                     RAFLKTDLNVSLFINFLRFKSKYKNLFQFRCDCQGYSKYVALEKIFEYEHSSRYNAGP
                     ETRRLLLNEMEGDEFHLSPLPGKLILNEYEEILEINENRSLENIEREFLRITDESQAG
                     IIREMMSDIQKFLITEPRLPSENKKFVTLPVPQIKPDDSREEIIEKVTSMSGLSEMCR
                     LTLYVYRQMDTIDWLPFVKAAIERNPVSFKELENLRIVEIYNLLQQMAHVSIYDGKRL
                     AMPDEVWNYRRGDGIEKAFLFACNIIERDKDACITLIIDKENVELIYSGNNYRFSSSK
                     GLKKQISIKGYEYQIF"
     gene            complement(4658..5080)
                     /gene="ndk"
                     /locus_tag="PLG42_02240"
     CDS             complement(4658..5080)
                     /gene="ndk"
                     /locus_tag="PLG42_02240"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOU30061.1"
                     /translation="MRSDITLAIIKPNAVRTGKIGPILAMINEAGFEIIALKMVKLTK
                     QQAESFYEVHREKVFFESLVEFMSSGPVVAMILRHRNAVDEFRKLIGNTDPAKAEPGT
                     IRKLYGISVQMNAIHGSDSIENAIKEASFFFSEIERFY"
     gene            5168..6184
                     /locus_tag="PLG42_02245"
     CDS             5168..6184
                     /locus_tag="PLG42_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOU30062.1"
                     /translation="MLELDKYKKELSDYLNNSDNILLICHINPDGDAIGSMLALYSFV
                     SSKGKRVDMISPNYLQEFLTWMKNSDRINIFIRDRKKCLRLIEEANLIIMLDFNQSDR
                     LAEAEKPVLKSSAKKVVIDHHLDSDNFYDLLISDASSSSTAEIVHELVCKLNDGFFAD
                     IGYAEAIYVGIITDTGNFEHGSFTGNTLRLIADLLDMGLNKERIYNLVFNNFTESRTR
                     LLGYALYQKMVVIPEYKTAYIALTREELERFKYSKGDTEGFVNLPLSIKGIDFSALFI
                     EKEGFVKISFRSRGDFSVNSFAAKYFQGGGHFNAAGGDYNDTLENTISYFLEMVKKER
                     NSED"
     gene            6189..6731
                     /locus_tag="PLG42_02250"
     CDS             6189..6731
                     /locus_tag="PLG42_02250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU30063.1"
                     /translation="MKNIILYIIILCQLISFSCNKPGEQVQKKKPGRRELEKVNTYLA
                     EKDRERILNYIERKNLKMTESSSGLWYCIINEGEGDFFKENDRIVMDYECTLLDGTKC
                     YSSYETGPKTVILGKSNIEAGLAEGLKMLKPGGEAIFIIPPFLGWGLLGDDRAIPPRA
                     VIVYEVKILNKKTPNRVQGD"
     gene            6788..7297
                     /locus_tag="PLG42_02255"
     CDS             6788..7297
                     /locus_tag="PLG42_02255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU30064.1"
                     /translation="MKIRLITLSILCLTFLWSCNLGKKYEEEEEIRIQNYLQSVGDTV
                     YTKTPSGLYYYIISEGSDRTPVNGDTISIWYKAKFLSGELFDTNLEQPDPYFFIVGAN
                     TVIKGIDEAVRYIKNGGKIKFITPSSLAYGAAGLYGYDAYGYYRVIVPGYTPFVWEVE
                     IDEIRAGSK"
     gene            7331..7825
                     /locus_tag="PLG42_02260"
     CDS             7331..7825
                     /locus_tag="PLG42_02260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU30065.1"
                     /translation="MKRFKPGISVLASIILIPTLFSACGEIGTWKEQEEQMIQDYLDK
                     LGDTVAIRKPSGLYYIELKEGTGLSPVPGDSVSFFYKGMFLDYVVFDTNLKDSIPYKS
                     KIGSNDLRAGIDEGLQYMKEGGKARLLTPSNLAFGPTGIWGIIPGYTPLIWEIELISV
                     KKAR"
     gene            complement(7985..8692)
                     /locus_tag="PLG42_02265"
     CDS             complement(7985..8692)
                     /locus_tag="PLG42_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOU30066.1"
                     /translation="MTGILYLIPVPIGSENFRKAIPDEVINITLSLRHFIVEKVRSAR
                     RYLKLLDRDFPVDESVFYTLNENTPVKELYDFLQPLLEGHNVGLMSEAGIPCIADPGS
                     MLVRMAHQKGIKVIPLPGPSSIYLALAASGLNGQKFTFNGYLPVKEDSLRKRLKELEK
                     RSEGGEAQIFMETPYRASRMLKYILETCNSATYLCIAVDITLPGEKIETKRIYEWKKN
                     IPDLNDHLVIFILQSAE"
     gene            8822..9586
                     /locus_tag="PLG42_02270"
     CDS             8822..9586
                     /locus_tag="PLG42_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU30067.1"
                     /translation="MKITFLGTGTSQGVPVIACDCKTCQSDDPRDKRLRTSLLLETEK
                     AIIVFDAGPDFRQQMLREKVTHIDAIVLTHEHKDHIAGMDDVRAFNYKTRDAIDIFAE
                     ERVLKEIRREYSYVFAEQQYPGIPKMRLNEINTENFSIKGLEIIPVRVMHFRLPILGF
                     RIGDFAYITDANSVPEESKEKLYGVKYLVVNALRKEKHISHFSLYEAINFIREISPRQ
                     AYITHISHQMGLYRDVSKELPPEIKLAFDGLSIEIS"
     gene            complement(9597..9815)
                     /locus_tag="PLG42_02275"
     CDS             complement(9597..9815)
                     /locus_tag="PLG42_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30068.1"
                     /translation="MMKCLEKILRFYSFFLFILLLLFTGCASSKQNPWAAKRAKASYV
                     STTQLGRNRYYFSTNYQKKLQKSMKKKK"
     gene            10297..11121
                     /locus_tag="PLG42_02280"
     CDS             10297..11121
                     /locus_tag="PLG42_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30069.1"
                     /translation="MRYELMFENRGTDTVTIGNVVPFGTDENSVFITGYGPSDLARAY
                     LFRPGYSKVRVILPDNAWELGYSSFNAGKYSVCALTRRQQTEGGRKRRYETVLPPGAS
                     VTYYMYVSTFQGEWQNGLKYMFRDRYLFDLENFDNELYQREDLKWIKNSYIIILQMAW
                     DRNFIDRTTGKYTYAGELAEWINLFGHIDVFGIWPTWPRLGLDRRNQWDLYRDLPGGT
                     QQLRAFARMSRLSGTRFFIAYNPWDRSTREEDHLKGMAQIIMETEADGVVLDTSGR"
     gene            11170..12783
                     /locus_tag="PLG42_02285"
     CDS             11170..12783
                     /locus_tag="PLG42_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU30070.1"
                     /translation="MYSEGMAVPADMPGIISGRVHNAIFLSPELNLNKLIKPDFSIFR
                     VCDVGEERIHREIAIAFFNGYGTELNLFRPGGRNREDLDYLAQTTFILRQNNDAFLDY
                     NWTPLIPTLIDKVYVNSWGSGNKTIFTILNMRHEGVYGELFETELPETVHLVSLWHHE
                     EITPVARNGKIKVRANASGWDPSLTGTRMEGSVDCIAQLPRLLKSILKGDTLFINKPD
                     NGTLILWQGNPSYSNKNSFHLKFRTDTALNLFNLTSGYEGKIVIQLLESGILKDENIL
                     YFKGGQPWLVSKVIRTKEAENIPDDMVLVPGARITYTLSTDEDFIPYPNVNQPINVEI
                     DSFLIDRYPVTNAQFYEFLINSGYVPSDTANFLKHWSNGIYRTGQEKYPVVYVSYEDM
                     QAYAKWAGKRLPTEAEWQLAAQGTDGRKWPWGNDFRGTWCNNSFGRPTPVDAFPKGAS
                     PYGVIDLVGNVWQMTNDMYCNGNYYFTVIRGGSFFNPESSWWYIKGGPQPLDRTQIML
                     MVSQGFDRSETVGFRCVKDITPGSIRTRK"
     gene            complement(12831..14261)
                     /gene="dnaA"
                     /locus_tag="PLG42_02290"
     CDS             complement(12831..14261)
                     /gene="dnaA"
                     /locus_tag="PLG42_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOU30071.1"
                     /translation="MNKSHTEVWNNCLKIIKDIIPSVSYKTWFEPIVPLKLENNVLTI
                     QVPSPFFYEYLEEQYIDILRKTLRREIGADAKLEYNVIMENSNYADGKPYTVKFPSRN
                     KTDLQNKPVQVPFELTQPSIKNPFVIPGIKKIHFDPKLNPDYNFNNFVEGECNRLARS
                     AGIAVGNNPGGTAFNPMMIYGDSGLGKTHLAQAIGILVKEKHPDKIVLYVNANKFQTQ
                     FTESIRNNNKNDFLHFYQMVDVLILDDVQEFAGKEKTQDIFFHIFNHLHQSGKQLVLT
                     CDKPPVELQNMEQRLLSRFKWGLLADLQKPDYETRLAILKKKAYNDGIELPDEIFEYL
                     AENITNNIRELEAALISLYAQATLNKKEITIDLARKMVEKLVSTSRKEITIEYIQKVV
                     CDYYKIPMEQMLGKTRKREIVQARQISMYFSKNLTKASLASIGSKIGGKDHATVLHAC
                     KTVNNLIDTDKHFRNQIHEIEKRLKL"
BASE COUNT         4474 a         2643 c         2679 g         4485 t
ORIGIN      
        1 tgccgtgttt tcctcccgac cgctctctag cctgttcaat gttgtttgtg gtaagaaccc
       61 cgaaaataac aggaatattg taatcgatat ttagctgggt aatacccata cttacactct
      121 ggcaaatata tgtgaaatgt ggcgtctctc cctgaatcac acaacccaga caaatgatcg
      181 catcaacgtc ctcattttct gccattaact gtgcgccaag ggtaagttca aaactgcctg
      241 gaacatgttt tataacaata tcttcatctg taacaccatg cttttttagt gtctttacgc
      301 acccatcaag aagtgaaaat gtaatttcct gattccagtc agaaacaact atgccaaatc
      361 tcatttcaga agcatcgggg acctttgacg ggtcataatc tgaaagattt gcagtagcca
      421 ttatttaaaa ggcaaaattt aaatttctga attttatcct ttttaaaaac cgtaaaaact
      481 ttataattcc tgcaggaaag ccttaacctc agttcatcaa aagctttacc ctggctatat
      541 atttatcaat tgttgcacct tctgtgcttt caggatattc gttttttatt ctttcgtaaa
      601 gcttcaatgc ttcagcatat ttttgctgca tttcaagtat ttgacctgcc ttcattagat
      661 acaaaggagt attaaacgaa ttattcgctt tttcagctgc ttcgatatat ctgtttattc
      721 cttcatcaag cctgccgagc tctaccaatg catcacccat cgctccaatt gccagtgaac
      781 caatgacatt atcttttatc gagtatttgc tcaaatattc aagtgcttcc tcatatttac
      841 caagatggag ataacatata cctgcacaat attttgcaag attacctgct ttcgttaacc
      901 tgtaagtttt cgcaatttct aagaatccaa gataattccc atctccgttc agcgcaagat
      961 tgagagaatc tatctccaac catctttcgg cctgatacat ctgtgactga gcttcctgat
     1021 ttctcttgtt gagataaact ctgctcagat aaaacagacc caccagaact acaagaattc
     1081 caagagttat aagaattact ttataatttt cttcaagaaa ctgttctgtt tttgtcagtg
     1141 tctcttctac actttttatt gattgcggac tttcagtttt tggttttttt gccatatcga
     1201 tgttctctca taatttgact tcgcaaatgt aaatttattt gctcatattt taaaattata
     1261 gtcttaaatt ttgataaata tttgtaaatg aacaacaaat atcacaactg acaaacattt
     1321 attattattt acaacagatg atattctttt agtaactttg ttgactgaaa atatctttgt
     1381 tctgtatata tttgattata tgtatattaa acaattgtcc ctaacaaatt tcaaaaacta
     1441 cgattcagct ttcctgaaat tttctcctgg aataaattgt cttgttggta ataatggggt
     1501 tggaaaaaca aatatactcg atgcgattca ttatttatca cttacaaaaa gtttttacaa
     1561 tatagtcgat agtgcaaaca ttaaacattc acaggaatat ttcatcatcg aaggcgttgt
     1621 tacggtaaat ggtaatgaag atattttgtt ttgttccttc caccgccaga agatgaagat
     1681 atttaagaga aatggcaagg aatacaataa attaaatgaa catgtgggta aattccctgt
     1741 agtgatggta tcacctgccg acagcatttt gattacagga gggagtgaag agagaaggaa
     1801 atttctcaac aaaatcatca gccagtacga tgccatatat cttgatgcaa ttcttaacta
     1861 caacaaagct ctccagcaga gaaacaggct tcttaaggat tatgccggca aaatatcagt
     1921 cgacagggag atacttgaag tatgggatgc ccagctgata agatatggag aatatatcta
     1981 cagagaacgc gccggattag tcaaagaact tatccccgtt tttaacgaat attattcatt
     2041 catatcagga ggaaatgaaa cagtaaacct cgattaccgc agccagctgt cagaatcaga
     2101 tttcagtgat ttacttgaaa aatccctgaa caaagacctg gtgctggaat ataccactgc
     2161 tggtatacat aaagacgatc taatatttga aatgggggga tatcctgtta aatcaacagg
     2221 ttcacaggga caacaaaaat catatcttgt ggcacttaaa cttgcaaaat ttgattatgt
     2281 gagacgaaga gtcggttttg ccccggtaat gctccttgat gatttattcg ataaattcga
     2341 cattgaaagg gtggagaaaa taattaaact tgttggtaat cacaggtttg gacagatatt
     2401 tataactgac accgatgaag aaagaatgaa aagcatactt tccaggctaa atgttgacca
     2461 caggctcttc aggattgaaa aaaacggtaa cattgaagta atttcagatt tcaataacta
     2521 tagcaatgag acgcagtaaa acaatttcgc tcgccgaagc tattaatgat tatatcaatg
     2581 aaatgaaaat cggcgggaag cttcaggaga ttaacataat cgattcctgg gaaaaaattg
     2641 taggtaaggc aatatcatca cgtacctcca gggtaactat taaaaacggc gtgctgacag
     2701 tctatcttaa atcatcagtt gtgaaaagcg aacttatgat gatacgcgag gccataaagg
     2761 aaaaaatcaa cagggaagtc ggcagcgaat ttattaaaga aatagtcctt agataaaatc
     2821 aaaatatttg atattcataa ccttttatac taatttgctt ttttaatcct tttgaagaag
     2881 aaaacctgta attgtttccg gaatatatta attcaacatt ctccttatca attattaatg
     2941 taatgcaggc atctttatca cgttcaataa tgttacaggc aaacagaaaa gccttttcta
     3001 tgccgtcacc tcttctgtaa ttccatactt catcgggcat cgcaagtctt ttaccatcat
     3061 atatgcttac atgtgccatt tgctggagaa gattgtaaat ctccacaatt cttaagtttt
     3121 ctagctcttt aaaacttaca ggattccttt ctattgcagc tttgacaaat ggcaaccagt
     3181 caatagtgtc catctgcctg taaacatata aagtcagtcg gcacatctcc gacaaaccag
     3241 acatgctggt aactttctca attatttcct ctctcgaatc atcaggtttt atctgaggca
     3301 caggcagggt cacaaatttt ttattttccg acgggagcct tggctcggta atgagaaact
     3361 tctgtatgtc cgacatcatt tcccttataa ttccggcctg actctcatct gtaatcctta
     3421 agaattctct ttcaatgttt tcaagcgacc tgttttcatt tatttccagt atttcttcat
     3481 attcgttaag tatcagtttg ccgggtaacg gactgagatg aaattcgtct ccttccatct
     3541 cattaagcaa caatcgtctt gtttcaggcc ccgcattata tctgctgctg tgttcatatt
     3601 cgaaaatttt ctccagagca acgtacttag aataaccctg gcaatcacat ctgaattgaa
     3661 acaaattttt gtattttgat ttgaacctca ggaaatttat gaaaagtgaa acattaaggt
     3721 cagttttcag aaaggcccgt agcttttctg aaaatgttct ataatcctca ggatttattg
     3781 ttgcaacatc gtacaatatg tggatgtaac ctgaaatatt tgagacaatt gtgacttttt
     3841 cgttttccag cgccctgcgt gccttggcag aaagggatgt cccgttaaac cacatgtttt
     3901 tggtcagtat ccttctgtta ttggttaata taccttcact ttcagttatg aaattttgtg
     3961 aatggagtgg tgtggccatt aagaatattt tttccagtgg aattctgcca attataaaca
     4021 tggcagcagc atacagggca gatagtgaga cacattcacc tgcacctgtt gtaaaacctt
     4081 ccttaaatct gaaggcttcc atcgcttcaa cagtctcagt cttccagtaa ggaataacat
     4141 ccgaatcata tttgtcaagg ccaaagtgct tccttatgtc aatatcaaaa taatatttgt
     4201 caccctcata tccgaacagg gcattcgact gtcttagggt gttcatgtct ataaaatcac
     4261 ggaacctctc aatctctttt gtcttggttg ttaaacccca tgtcgcaagg tcaaggttaa
     4321 atatgtaatt ctgaataata tactgtttta ttctgtttac tttgtaaaga aatttcttat
     4381 tgtaaatccc gtcaaaccac gaatcctccc tccatttcca gattacaggc gacataatat
     4441 tcgcaagaac aagcggataa agcaattcgg gaaaaacaaa cacctccatg tcggaaagtg
     4501 taaatgccga agaatatttt tccagatttc ttttatcact gaaatcaaca ggataactct
     4561 gactcatttt aataaaaaat tgctgcgaag atatatctta tctggataat gagaaaataa
     4621 ttcgattttt aaaatattat ggagtaaaga gcgaaatcta ataaaatctc tcgatttcag
     4681 aaaagaagaa actggcttct ttgatggcat tttcaatcga atctgaaccg tgtattgcat
     4741 tcatttgaac ggatatccca tacaattttc ttattgtacc gggttcagct tttgccggat
     4801 cagtattgcc tatgagtttt ctgaattcat ccacggcatt cctgtgtctc aatatcatag
     4861 caacaacagg ccctgacgac ataaactcta ccagactctc aaaaaatact ttttcacgat
     4921 gtacttcata aaaagattct gcctgctgct ttgtaagctt taccattttt aaagctatta
     4981 tttcaaaacc ggcttcatta atcattgcca gaataggtcc gatttttccg gttcttactg
     5041 cattaggttt tattattgcc agcgtaatat cacttctcat actaaattta tcgtttaata
     5101 tattttcttt ttatgatgca atattaatat ttaataactt taccgccctt actaagtctt
     5161 aaataaagtg ttagaactcg acaaatataa aaaagaatta tcggattatc tgaacaattc
     5221 tgataatata ctcctgattt gccatattaa tcctgatggt gatgcaatcg gttcaatgct
     5281 tgctctttat tcttttgttt cgtctaaagg gaagagggtt gatatgattt caccaaatta
     5341 ccttcaggaa ttccttacat ggatgaaaaa ttctgacagg attaatattt ttataaggga
     5401 ccgtaaaaaa tgtctgaggc ttatcgagga agctaactta ataattatgc ttgattttaa
     5461 ccagtcggac cggctggcag aagctgaaaa accggtttta aaatcatcag ccaaaaaggt
     5521 tgtaattgat catcatcttg attcagataa tttttatgac cttttaattt cagatgccag
     5581 cagctcatct actgcagaaa ttgttcatga attggtgtgc aagctcaatg atgggttctt
     5641 cgccgatatt ggttatgccg aggctatcta tgttggtatt atcaccgaca caggtaattt
     5701 tgaacatgga tcatttaccg gcaacacatt aagattaatt gccgacctgc tggatatggg
     5761 cctcaacaaa gagagaattt acaatctggt tttcaataat tttactgaat cccgaaccag
     5821 gcttcttggt tatgccctgt atcagaaaat ggttgtcata ccggaatata aaaccgcata
     5881 tattgctctt acacgggaag aactggagag attcaaatac agcaaaggtg atacagaagg
     5941 atttgttaac cttcctctat caataaaagg tattgatttt tctgctctct ttatcgaaaa
     6001 agaaggattt gtaaagatat ctttcagatc aagaggggat ttttcagtaa actcctttgc
     6061 cgctaaatat tttcagggtg gaggccattt caatgctgct ggaggtgatt ataacgacac
     6121 actcgaaaat accatcagct attttctcga aatggttaaa aaagaaagga acagtgaaga
     6181 ttaaatgttt gaaaaatatt attctgtata tcattatttt atgccagctt atatcttttt
     6241 cctgcaacaa accgggagaa caggtacaga aaaaaaagcc cggaagaaga gaacttgaaa
     6301 aagttaatac ctatctggca gaaaaagata gggaaagaat ccttaattat atcgaaagga
     6361 aaaaccttaa aatgacagag tcaagctcag gactgtggta ttgtataatc aatgaagggg
     6421 aaggggattt cttcaaggaa aatgacagga ttgtaatgga ttatgaatgc actctgcttg
     6481 acggtacaaa atgttattca tcatacgaaa caggccctaa aaccgttata cttgggaaaa
     6541 gcaatataga agccggactt gcagaaggac ttaaaatgct gaaacctgga ggggaagcaa
     6601 tattcataat accacctttt ctgggatggg ggctcctggg tgatgacagg gcaatacctc
     6661 ccagagcggt tatagtatat gaagtgaaaa tacttaataa aaaaacacca aaccgcgttc
     6721 agggagatta aacttaggca aaattgaata aatttgtaaa aaattaaagg gattaaaaga
     6781 ttgtgctatg aaaattagat taattacatt gagcattttg tgtttaactt ttttatggtc
     6841 gtgtaatttg ggcaagaagt atgaagaaga agaggaaatc cgaattcaga attatttgca
     6901 atcagtagga gatacggttt atactaaaac ccccagtggt ttatattatt acatcatctc
     6961 tgaaggctca gacaggacac cggttaatgg ggatactata tcaatatggt ataaagccaa
     7021 gtttctttct ggtgaacttt ttgatacaaa tctggaacaa ccggatccgt acttttttat
     7081 tgtaggtgca aataccgtaa taaaaggtat tgacgaagcc gtaaggtaca taaaaaatgg
     7141 aggaaaaatt aagtttatta ctccctcaag ccttgcatat ggagcggcgg gtctttatgg
     7201 atatgatgcc tatggatatt accgtgtaat tgttcctggt tatactcctt ttgtctggga
     7261 agttgaaata gatgaaatta gagctggctc aaagtaaggt ttttttcttc tgaatgttct
     7321 aaaaatgtgc atgaaaagat ttaaacctgg catttcagtc ttagcttcaa tcattctaat
     7381 tccgacgctt ttttctgcct gcggggaaat aggaacatgg aaagaacagg aagaacagat
     7441 gattcaggat tacctggata aacttggtga tactgtagca attcgtaaac ccagtgggtt
     7501 gtattacatt gaattgaagg aaggcaccgg tctttctcct gttccgggcg attcagttag
     7561 cttcttttac aaaggtatgt ttctcgacta tgtagttttt gatacaaatc tgaaagacag
     7621 cattccctat aaaagcaaaa ttggttcaaa cgatcttcgt gcaggaattg atgaaggcct
     7681 tcagtatatg aaagaaggag gtaaagcccg ccttctaacc ccgtcaaatc tggctttcgg
     7741 tccaaccgga atttggggta tcataccggg atatacaccc cttatatggg aaatagagct
     7801 tatttctgtt aaaaaagcaa ggtaaacgta gcaatcatta ataacatctg cgaaggctta
     7861 tttaattaaa ctataataat tactcttcgc aatgaatttt aattcgttat taatgttcta
     7921 ttaaattatt aattaatagt taattataat tattaatctt cgtgaggata gatcaataaa
     7981 aagtttattc tgccgattgt aaaatgaaaa ttaccagatg atcgtttaaa tcaggtatat
     8041 ttttcttcca ttcataaatt ctctttgttt caatcttttc acctggcagg gtaatatcaa
     8101 cagcaataca taaatatgta gctgaattac aggtttcaag aatatatttc agcattctgc
     8161 tggctctgta tggagtttcc atgaatatct gggcttcacc tccttctgat cttttctcca
     8221 gttcttttaa tcgctttcta agactatctt ccttaaccgg aagatagccg ttaaatgtaa
     8281 acttctgacc atttagcccc gaggcagcaa gcgctaaata tattgaagaa ggaccgggaa
     8341 gagggataac ttttattcct ttttgatgag ccatcctgac taacatcgac ccggggtccg
     8401 ctatacaggg aatacctgct tcactcataa gcccgacatt atgaccctca agcaacggct
     8461 gaagaaagtc ataaagttct ttcacagggg tgttctcatt gagtgtataa aatacagatt
     8521 catcaacagg aaaatccctg tcgagcagtt tcagatatct tctggcactc cttaccttct
     8581 caacaataaa atgcctcaac gacagagtaa tattaattac ttcgtcaggt atagcttttc
     8641 tgaagttttc actgccgatt ggtacaggta tcagataaag aatcccggtc aaaacataat
     8701 actttttagt actttgtaaa gatatatatt taaaaaatat agcctcgttt caaaaaacaa
     8761 gatatctgtt attgactgtt tctaaatttt gagttaggtt tgtgcatcaa tttttataaa
     8821 agtgaaaatc acctttctgg gaaccggaac atcacaggga gtaccggtaa tagcatgtga
     8881 ttgcaaaacc tgtcaatcgg atgacccaag ggataaaaga ctcagaacat ccctgctgct
     8941 tgaaactgaa aaggcaataa ttgtctttga tgcaggacct gacttcaggc agcaaatgct
     9001 cagggaaaag gttactcata tcgacgcaat tgttctcaca cacgaacata aagaccatat
     9061 tgcaggtatg gatgatgtgc gagcatttaa ctataaaacc cgcgatgcaa tcgatatatt
     9121 tgccgaagaa agagttctta aagaaataag gagagaatat tcttatgtct ttgctgagca
     9181 acaatatccg gggattccca agatgagact aaatgaaata aatactgaaa atttttcaat
     9241 caaaggtctt gaaattattc ctgtaagggt aatgcatttc cgcttaccta ttctgggatt
     9301 cagaattggt gatttcgcat atattactga tgcaaacagt gttccggaag agagcaaaga
     9361 aaaattatat ggagttaagt atttggtagt aaatgcttta aggaaagaga aacatatttc
     9421 acatttcagc ctatatgagg caattaactt tattagagaa atctcaccac gtcaggcata
     9481 tattacacat ataagtcacc agatgggatt gtatagagat gtgtcaaaag aactccctcc
     9541 cgaaatcaaa cttgcatttg acggattgag tatcgaaatt agttaaaatc attctcttac
     9601 tttttctttt tcatgctctt ttgaagcttc ttctggtaat tggttgaaaa ataatacctg
     9661 tttcttccaa gctgcgttgt acttacatag gatgccttgg ctcttttggc agcccatgga
     9721 ttctgctttg aagaagcaca tccggtaaac aaaagcagga gaatgaacaa aaaaaatgaa
     9781 taaaaccgta atattttttc cagacatttc atcattaaag ggtcttcaat accagatata
     9841 aatataaata ttttaattat ttaacgaaaa aaactcattt tggttacttt tttcctgtaa
     9901 aaattttttt cttttgctgt taactcacta attttcattg taaatacgaa atgtatttta
     9961 ggtatgtgtg caagaatcta tataaactca ctggtagcca gaaggattaa gatatgttta
    10021 atttatcttt ttttgtttca ttcaatttta attgctcaga aaatatctga tgttcatttt
    10081 acagtatcag ccggtttctc agcttatcgt atttcattgt cagacagtat tttatatgat
    10141 tttaaaaaaa tattgcctgt ctttactttt gaactaaata gtaaacagtt aagttctaaa
    10201 gattttgatg taaaaataat tgatggtacc tgggagcaga atttcggccc ggcgatacat
    10261 acatccttaa aaaggttatc agggttttcc gatgcattga gatacgagct tatgtttgaa
    10321 aatagaggga cagatacagt cactatcgga aatgttgtcc ctttcggaac agatgaaaat
    10381 tctgtgttta ttactggtta tggtccttct gacctcgcaa gggcttacct attccgacca
    10441 ggatattcaa aggtgagggt gatattgcct gataacgcgt gggaacttgg gtactcttct
    10501 ttcaatgcag gaaaatattc ggtatgcgca cttaccagac gtcagcagac tgagggtggc
    10561 agaaaaagaa gatacgaaac tgtactcccg cctggggcta gtgtaacata ttatatgtat
    10621 gtgagcacat ttcagggcga atggcagaat ggtttaaaat atatgtttcg tgacagatat
    10681 ctttttgacc ttgaaaattt tgataatgaa ctgtaccagc gcgaagatct aaaatggata
    10741 aaaaacagtt atatcattat tctccagatg gcatgggaca gaaactttat cgacagaacc
    10801 acaggaaaat atacctatgc cggtgagctc gcagaatgga ttaatctgtt cggacatata
    10861 gatgtgtttg gtatatggcc tacatggccc aggctcggac ttgacaggcg aaatcaatgg
    10921 gacctttaca gggatctgcc aggtggaacc cagcaactca gagctttcgc aagaatgtcg
    10981 cgtctgtcag gaaccaggtt ctttattgca tataacccat gggacagaag cacacgcgaa
    11041 gaagaccatc tgaaaggtat ggctcaaata attatggaaa cagaagccga tggtgtggtt
    11101 cttgatacaa gcggccggta aagccgcgag attcaggctg cagccgacag tgtaagaaaa
    11161 ggtgtaatta tgtattccga aggtatggct gtacctgctg atatgcccgg gatcatatcg
    11221 ggccgagtac ataatgctat tttcctgagt cctgaactta atctgaataa acttatcaaa
    11281 cccgattttt caattttccg tgtgtgtgat gtgggcgaag aacgaataca ccgtgaaatt
    11341 gcaatagctt tctttaacgg ctatggtact gaactgaacc tcttcagacc aggaggaaga
    11401 aatcgcgaag accttgatta ccttgctcaa acaactttta tactcaggca gaacaatgat
    11461 gcctttctag attacaactg gaccccactt attcctacac ttatcgataa agtatacgtt
    11521 aatagctggg gttcaggaaa taaaacgata tttacaatac ttaacatgag gcacgaaggt
    11581 gtatatggag aattatttga aactgaactc cctgaaacgg tacacctcgt atcactctgg
    11641 catcatgaag aaattacacc cgtcgcccga aacggaaaaa taaaagtaag agcaaacgct
    11701 tcaggatggg atccatccct gacaggtacc agaatggagg gatcggtaga ctgcattgca
    11761 cagctgcctc gtcttctgaa atctatattg aaaggagata cactattcat aaataaacct
    11821 gataatggca ccctgatcct ctggcagggc aacccttcat acagcaataa aaattcattt
    11881 catcttaaat tcagaacaga tacagctctt aatttattta atcttacatc gggttatgaa
    11941 ggaaagatag taattcaatt acttgagtca ggtattctta aagatgaaaa tattctctat
    12001 ttcaaaggag gacaaccgtg gttggtaagc aaggtaatcc gtacaaaaga agcagaaaat
    12061 atcccggatg atatggttct tgttcccgga gctagaatta catacacact ttcaacagat
    12121 gaagatttca taccatatcc taatgttaat cagcctatta atgtagaaat tgatagtttt
    12181 cttattgaca ggtatccagt taccaatgca cagttttatg aatttcttat caattccggt
    12241 tatgtgccgt cagatactgc aaatttctta aagcactggt cgaatggcat ttaccggact
    12301 ggccaggaga aatatccggt agtgtatgtg agttatgaag atatgcaggc ttatgcgaag
    12361 tgggcaggaa agaggttgcc aactgaagca gaatggcaac tggcagcaca ggggactgat
    12421 ggaagaaaat ggccatgggg aaatgacttc aggggaacat ggtgcaacaa ttcatttggt
    12481 cgcccaactc cggtagatgc attccccaag ggggcaagcc cttacggtgt tatagacctt
    12541 gtgggtaatg tctggcaaat gaccaacgac atgtattgca atggcaatta ctattttacc
    12601 gttatccggg gtggaagctt tttcaaccct gaatcgagct ggtggtatat aaagggaggt
    12661 cctcagcctc tcgaccgaac acagattatg ctgatggtgt cacagggctt cgatagaagt
    12721 gaaacagtag gtttcagatg cgtaaaagat ataactcctg gttcgatacg aaccagaaaa
    12781 tgattgtttt accagaaata ttttataaag ctcaggacta actttagctg ctaaagctta
    12841 agcctttttt caatttcgtg tatctgattt ctgaagtgtt tgtcagtgtc aatcagattg
    12901 tttaccgttt tacaggcatg aagcacagta gcatgatctt taccacctat ttttgacccg
    12961 atgcttgcca gagaagcttt tgtgaggttc tttgagaagt acattgatat ctgccttgcc
    13021 tgaacaattt cgcgttttct tgtcttgcca agcatctgtt ccataggtat tttgtaataa
    13081 tcacaaacta ccttctgaat atattcaatg gttatttctt tccttgaggt gcttacaagc
    13141 ttttctacca tctttctagc aaggtcaatg gtaatctctt ttttattaag agttgcctga
    13201 gcatagagtg aaataagggc agcctcaagt tccctgatat tattggtaat attttcagcc
    13261 agatattcaa atatttcatc aggcagctct atgccgtcgt tatatgcttt ctttttcagt
    13321 atagccagac gtgtttcata atctggcttc tgcagatcag caagcaatcc ccatttaaac
    13381 cttgagagca gacgttgttc catgttctgc aattcaaccg gaggcttgtc gcatgttagc
    13441 acaagctgtt tacccgattg gtggagatga ttgaatatgt ggaagaaaat atcctgtgtt
    13501 ttttccttcc ccgcaaattc ctgaacgtca tcaagaatca atacatctac catttgataa
    13561 aaatgcagaa aatcattctt gttgttattt cttatcgact cagtaaactg cgtctgaaac
    13621 ttatttgcat ttacatataa aacaattttg tcgggatgtt tttcctttac cagtattcca
    13681 atcgcctgag caaggtgggt ttttcccagc cccgaatcac cataaatcat catcgggtta
    13741 aatgcagtac ctcctggatt atttccaaca gcaatccctg ccgaccgagc aagcctgtta
    13801 cattcaccct cgacaaagtt gttgaaattg taatccggat tcagcttcgg atcgaaatgt
    13861 atctttttta ttccaggtat tacaaatgga ttcttaattg aaggttgtgt aagctcaaaa
    13921 ggtacctgca caggcttgtt ctggaggtca gttttatttc ttgaaggaaa tttgacagta
    13981 tacggcttgc cgtcagcata atttgaattt tccattatga cattgtactc cagtttggcg
    14041 tcagctccta tttcacgcct tagagtcttc ctgaggatgt caatatattg ctcctcaaga
    14101 tactcgtaga aaaacggact tggtacctga atggtaagta cgttgttttc aagcttcagg
    14161 ggaacaatag gttcaaacca tgtcttataa cttactgaag gaataatgtc cttaatgatt
    14221 ttcaaacagt tattccagac ttcagtatgc gatttattca tagctcttca gtttgatttt
    14281 t
//
LOCUS       DAOSNB010000027        14099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4342_length_14099_cov_5.283680, whole genome shotgun sequence.
ACCESSION   DAOSNB010000027 DAOSNB010000000
VERSION     DAOSNB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14099
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4342_length_14099_cov_5.283680"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2466)
                     /gene="secA"
                     /locus_tag="PLG42_02295"
     CDS             complement(<1..2466)
                     /gene="secA"
                     /locus_tag="PLG42_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOU30072.1"
                     /translation="MSIIGKILSLFLGNKYERDLKELYPYVELINREFEKLRDLTNDQ
                     LRDKSEALRNEIRESIREDEEEVRMLKEKAEAEEDVNVKEELYNQIDKLEKQIDKKLE
                     EILTQHLPLAFAIVKETARRFKENSELEVTARQYDRDLAAKKPHITIKGDKAYWSNRW
                     MAGGNETVWDMVHFDVQLMGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGRGVHI
                     VTVNDYLAKRDSEWMGPIYEFHGLTVDCIDKHQPNSPDRRKAYQADITYGTNNEFGFD
                     YLRDNMAVNPEDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPTAQAENQQFEELKPF
                     VEKLVSAQKKLVNQVLIDAKKHIAEGNLEQGGMLLLRAYKGLPKNSALIKYLSEEGIK
                     SLLIKTENFYMQNNSKDMPKVTDELYFIIDEKANTIELTDKGIDLISASVSDPSFFIL
                     PDVGSKIAELEKSGLSEREKLKAKDEILQDYSVKSERVHTLTQLLKAYTLFERDVEYV
                     VIDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFRMYH
                     KLAGMTGTAETEAAEFWDIYKLDVVVIPTNVPCIRIDKEDIVYKTKREKYNAIIEEVI
                     KLHKQGRPVLVGTTSVEVSELLSRMLKMKGLKHNVLNAKLHQREAEIVAEAGKSGTIT
                     IATNMAGRGTDIKLTPETRAAGGLAIIGSERHESRRVDRQLRGRSGRQGDPGTSQFFV
                     SLEDELMRLFGSERIAGIMDRLGLKDGEMIQHPMITKSIERAQKKVEENNFGIRKRLL
                     EYDDVMNSQREVIYKRRRHALLGE"
     gene            complement(2711..3091)
                     /locus_tag="PLG42_02300"
     CDS             complement(2711..3091)
                     /locus_tag="PLG42_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU30073.1"
                     /translation="MAGILIVEDEKDVREMLRDALIRRKYSVIEAVNGKEALAKFKAS
                     FINIVITDILMPDEDGLKVIMKLKELKPDLKIIAISGGGKFGPAGYLNIARTLGADEV
                     FTKPFSLRELEKKIEELLDAGKQI"
     gene            complement(3101..4996)
                     /locus_tag="PLG42_02305"
     CDS             complement(3101..4996)
                     /locus_tag="PLG42_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU30074.1"
                     /translation="MTVEKNYIDKHLYDHLINNSRSMLSVINRNYCYEKVNNTFCRYH
                     NIQPENILGKSLADLWGEDTFKENIKENIDICLNGNIVRYEANFQIPLYGERFFEVIF
                     RPLPDEEGNVTHLLAETVDITDLKITQRAISEMEEEFRRLETHLPIGLLRCKPDGTII
                     HYNKAFSDILENTENENLEGLNIKDFYREKTIFDVQIMHAADGSISTFKRVPLITFKG
                     REIRCRVNIYVVTEKDTKNPLYADFAFEDSTREIMLEHRLLQAKNIETIGSLAGGIAH
                     DFNNILSSIFGYSEMLLEEIKDNQEGTEMVGKIIRAVSKARELTNQILTFSRQVGQEK
                     IPVNICDLLDEAVSFAESVKKPNVSIEKNFRDRNVLVYADPTQLFRVFMNLIMNALQA
                     MENTGGTLTIVQERINGNQFQSESGKSIIADEYTVISFEDTGTGMEESVISRIFEPYY
                     TTKDVGKGAGLGLSVAYGIITELDGEITVKSVKNKGSQFKIYLPLADSLPLEEIAPFA
                     ERSRVLFICGGSHELKVLGSALEKSGYAVDYAIDIDGLIKLSGDEDAKPDVIIFSQDN
                     ENISSDILSEYMIEKNIDIPVVLITDSSYDLSKEKLLNSGIAKNVLFKPVSLREILTS
                     IQISLNK"
     gene            complement(5030..6769)
                     /locus_tag="PLG42_02310"
     CDS             complement(5030..6769)
                     /locus_tag="PLG42_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOU30075.1"
                     /translation="MENFIVSARKYRPSTFDMVIGQDPITSTLKNAIKNRQIAHAYLF
                     CGPRGVGKTTCARIFAKTINCFNIQENGEACDKCESCVSFNTLRSFNIHELDAASNNK
                     VEDIRTLIEQVRIPPQIGKFSVYIIDEVHMLSSSAFNAFLKTLEEPPSHAIFILATTE
                     KHKIIPTILSRCQIFDFQRIRVEDIVERLKYVAKNESVSADENALHVIAMKADGSMRD
                     ALSIFDQLVSYSGNSITYETVLEHLNVLDYEYYFRTVDAALAGDVAKILLTFNEVLTR
                     GFDGHNFIAGLNSHFRNLLVCKDEVTLKLLDTAPAIREKYLSQSRKCSREFIYRALEI
                     GSNCDLGYKSSKNQRLHVELALIKLCNISETEPGETEKKKSQLKPEKKDIHTLQPQEP
                     KQSQKQEVKSDILIARESKPPENKIVSNERSLSIKELINGFHDTQTEEKVPTEIKTDE
                     LPVVASSKEKFDPDEFLKLWKKFTETVKNEGPRVSGMFKSLKVEVHKDKLVTIHLNNI
                     AQKELFNRNYKQRLLAFMEEYYGKDFFEIETVVDPVESEDIIYSDEQKYQFLTSKYPA
                     IKDIRKKFNLDFE"
     gene            6979..7329
                     /gene="rpsF"
                     /locus_tag="PLG42_02315"
     CDS             6979..7329
                     /gene="rpsF"
                     /locus_tag="PLG42_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOU30076.1"
                     /translation="MNHYETVFIATPVLSENQMKEAVQKFKKIITENSGEIIHEENWG
                     LKKLAYPIKKKSTGFYYLIEFKGPGDIVEKLELQYRRDERILRFLTFKMDKYAIEYAE
                     KKRKQKEVEKITEE"
     gene            7333..7602
                     /gene="rpsR"
                     /locus_tag="PLG42_02320"
     CDS             7333..7602
                     /gene="rpsR"
                     /locus_tag="PLG42_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOU30077.1"
                     /translation="MAQNESEIRYLTPPAVEIKKKKYCRFKKNRIKYVDYKDPEFLKK
                     FLNEQGKILPRRITGTSLKYQRRVAKAVKRARHLALLPYVTDLLK"
     gene            7613..8059
                     /gene="rplI"
                     /locus_tag="PLG42_02325"
     CDS             7613..8059
                     /gene="rplI"
                     /locus_tag="PLG42_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOU30078.1"
                     /translation="MEVILLQDVPQLGHKDEIVKVKDGYGRNYLIPKGMAIAATPSAK
                     KVHAENLKQRAHKEEKIRLEAQALAEKLQGINLVIGAKTSSTGKIFGSVNAIQIAEAL
                     KEKGFEIDRKNIMLTADQIKEVGKYKATIKLHRDVKAEIEFEVVSV"
     gene            complement(8075..8536)
                     /locus_tag="PLG42_02330"
     CDS             complement(8075..8536)
                     /locus_tag="PLG42_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOU30079.1"
                     /translation="MNRLFVWILVSAVFFSSCNTQVKKQDSPLRRVITLENTPVSRPY
                     SPAVEVGNMLYVSGQIALDPATGKLIEGGFEEQARQTLSNLKTVIEKAGYKLEDVVRC
                     TVLLTDINNYAPMNRIYMEYFPSDPPARMAFAVRDLPMGALIEIDAIAAKK"
     gene            complement(8543..8926)
                     /locus_tag="PLG42_02335"
     CDS             complement(8543..8926)
                     /locus_tag="PLG42_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU30080.1"
                     /translation="MKRIICTSNAPVAAGPYSQAVEAGGILFVSGQVALDPATGKLIE
                     GDIREQTEQVLRNIGAILEAAGYTYRDVVKSTVFLSNIQDFKTMNEVYSKFYPSEQPA
                     RVALAVKDLPLGALVEIEAIAIKSK"
     gene            complement(8964..11567)
                     /locus_tag="PLG42_02340"
     CDS             complement(8964..11567)
                     /locus_tag="PLG42_02340"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOU30081.1"
                     /translation="MFSQEEGKALPDTLFVLKDSILTDTILKPLKISPDAVDRTITHT
                     AKGYRRTDMVNRKVYLVEEAVVTYGDITLTADSIVLNTETGIIYATGRPDSTGKMVGR
                     PVFKQGEESFESEALEYNFRTRKGRVFNLVTQQEEGYLRSKVTKRLDDGSLNIGRSTY
                     STCDADPPHFYIGFNKAKVVPAKKIVTGPAYLVLEGIPLPVVIPFGYFPLYRKNAAAS
                     GIIMPKIGTTYELGYSLRDGGYYFAINDNIDLSLTGTLYTNGTWMVSASSNYFKRYKY
                     SGRLFLSYANNVTGHMGLPDYSKTKNYRIDWTYSQDPKASPGSRFSASVSMSSSLYDR
                     QNSYVIAEHVNTTRQSSISYSKTWEGTPFNFTASLNHSQNVQNKTMSLNLPQATFSMS
                     RIYPFKNRKRVGPSRWYEDLQFQYTAKLDNRIYNIADSLLFTRSLFNNMQSGFRHDAP
                     LSLQIRPFKKIPGFIISPQLSYSGVLYTQKYNKRLVTDYFDPELGKTRPVVITDTLRG
                     FFYGQSVNTSISTGINPQLFGMYQFKPGSRLEAIRHVIKSSISFSYVPVIKGLTTNMY
                     RQYTDVNGVTRTYSIFEGNIHGTPSAGKRSGGISFSLVNIVEAKIHDKNDTTGVAKKV
                     KIIENLSAGTYYNIFADSLRWSPVGVSFRTVLLNNIGLSANSSFSLYGTNENGQVINK
                     FYFKQTGKPLRLTGFNVSVDFDVNQLFSRKKTGATNTPPGMVSRGGYGEEEPVSGPSA
                     QRAYQDERESDSDYPRFDMPWSMHLAYSMNYSKQALTSSVIQSLSLTGDLSLTKKTKI
                     TYETGFDFKNKQITTTRIGIYRDLHCWDMSFDWIPVGTMKSWYFTIKVKASVLADLKY
                     ERRKDFRDQFY"
     gene            11704..12855
                     /locus_tag="PLG42_02345"
     CDS             11704..12855
                     /locus_tag="PLG42_02345"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOU30082.1"
                     /translation="MVVTIKNGVLNYSIICFYLVLLLLSICNSVYAGSGYQQPVKEKW
                     IIVIDAGHGGRDPGAIGSFSYEKDITLAIALKTGELIEKNISNAEVIYTRKTDVFMDL
                     AERANLANKNKADLFISIHANWHRYRNTYGAETYVMGHHKDQQNLEVAMKENEVILLE
                     KDYTTKYEGFDPKSPESYIIFTLMQSAFFEQSTDLASKIQVQFREQIKRFDRGVKQAG
                     FWVLYMTTMPSVLIETGFISNSAEEKYLNSKKGQEEIAEAIFRACSDYIAEVYKKSQI
                     SAIRIDNNASSEGNAGAPQSNIVFMVQVLSSTRKTEITPKNFKNLTDITEIHDGNRYK
                     YAAGKFSDYNQAVAYRRKIESLFPDAFVIAVKENKILPLQDALNELKKK"
     gene            12866..13807
                     /locus_tag="PLG42_02350"
     CDS             12866..13807
                     /locus_tag="PLG42_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOU30083.1"
                     /translation="MKFKISNELKVGVIAIITILAFIYLYNYLKGKDLFSTMASYYVI
                     YDDISGLTESSPVEINGFKAGVVQTINLMNDNTGRLLVELSIKKNYAIPRGSVAEITS
                     VSLIAGMKVRLLYGPGPEVYRNGDTIPGRLSPPILTTFEKDFSVIREKVNNLIDELDT
                     AISGINDLLSPEFRSNLKGSISNINQTTASLSEIMGTKKTELKDAVDNFTAFSSMLAS
                     SSGKIDRTVSNLQAITDSISASGLYESLTSLKMTLEETRTLLDGINKGKGTAGQLVNN
                     DTLYRNLSSSLESLDLLLKDLKENPKKYVHFSIFGRK"
BASE COUNT         4119 a         2747 c         2589 g         4644 t
ORIGIN      
        1 ttctcccaga agtgcgtgtc gtctgcgttt atagataacc tcacgctgtg agttcatcac
       61 atcgtcatat tcaagcaaac gctttcttat gccaaagttg ttctcttcta cttttttctg
      121 tgctctttcg attgattttg taatcatggg gtgctggatc atctcgccgt ctttaagacc
      181 cagtctgtcc attatccctg caattctctc cgaaccaaaa agtctcatga gttcatcttc
      241 gagagaaacg aagaactgcg atgtacccgg atctccctgc ctgcctgacc ttcctctgag
      301 ctgacggtcg actcttcttg attcatggcg ttctgagcct atgattgcca gacctcctgc
      361 ggctctggtt tccggggtaa gtttaatatc cgttccccta cccgccatgt ttgttgctat
      421 ggttattgtc ccgctttttc ctgcttctgc aactatttca gcttcacgct ggtgaagttt
      481 tgcattcagc acattatgct tcagaccttt catcttcagc atccggctca gtaattctga
      541 aacctctact gaggttgttc ctaccaggac tggccttccc tgcttgtgaa gctttataac
      601 ttcttcaatt attgcattat acttctccct ctttgtctta taaacaatat cctctttgtc
      661 aattcttatg cagggtacat ttgtaggtat tacaacaaca tcaagtttgt atatatccca
      721 gaattctgcg gcttccgttt ctgctgtgcc tgtcattccg gcaagtttat gatacatgcg
      781 gaaataattc tgaagcgtta ttgtagcata tgtttgtgtt gcggcttcaa ctttaacatt
      841 ttcctttgcc tcaatagcct gatgcagccc gtcggaatac cttctgcctt caagtattcg
      901 tcccgtctgt tcgtcaacaa tctttacctt gttatctatc accacatatt cgacatccct
      961 ttcgaaaagg gtatatgcct ttaaaagctg agtgagggta tgaactctct ctgacttaac
     1021 tgaataatcc tgaagaattt catccttggc cttaagtttt tcgcgctcac ttagtcctga
     1081 tttttcaagt tcagctattt tgctgccaac atcgggtagt atgaaaaacg aaggatctga
     1141 cacagaagct gatataaggt caataccctt atcggtaagc tctatggtat tggccttttc
     1201 atcgataata aaataaagct catcagtgac tttaggcata tccttgctgt tattctgcat
     1261 ataaaaattc tcggttttta taagcagtga ttttatgcct tcttcactca ggtatttaat
     1321 aagtgcacta tttttaggta accccttata ggctctaaga agaagcatcc ccccctgttc
     1381 cagattacct tctgctatat gtttctttgc atcaataagt acctgattaa caagtttctt
     1441 ctgagctgaa acaagttttt caacaaaagg tttcaactct tcaaactgct ggttctcagc
     1501 ctgtgcagtt ggacctgaaa tgatgagtgg tgttcttgca tcatcaatca acactgaatc
     1561 aacttcatcc acaatagcat aatgatgttt tctctgcaca agatcttcag gattgactgc
     1621 catattatcg cgcaggtagt caaatccaaa ctcattgttg gtgccgtaag taatgtcggc
     1681 ctgatatgct ttgcggcggt cgggtgaatt gggctgatgt ttatctatgc aatcaacggt
     1741 aagtccgtga aattcgtata tggggcccat ccattctgag tcgcgtttag caaggtagtc
     1801 atttactgtt acaatgtgga ctccccttcc agccaatgca ttcaggaaaa caggcaatgt
     1861 ggcaacaagt gtttttcctt ctcccgtagc catttccgca attttaccct ggtgtagaac
     1921 cacacctccc attaactgga catcaaaatg caccatgtcc cacactgttt cgtttccacc
     1981 tgccatccac cggttactcc agtaggcctt atcaccttta attgtaatgt gcggcttttt
     2041 tgcagcaaga tccctatcgt attgccgggc tgttacctcg agttcactgt tttccttaaa
     2101 ccttctagcc gtttctttga caatagcaaa agcaagagga agatgctgcg tcaaaatttc
     2161 ttcaagcttt ttgtcaatct gcttctccag cttgtcaatc tggttgtata attcttcttt
     2221 aacgttaaca tcttcttctg cttcggcctt ctcctttaac attcttactt cttcctcatc
     2281 ttctctaata gattccctga tttcattcct cagggcttcc gatttatccc tcaactggtc
     2341 atttgtaaga tctctgagct tttcaaactc cctgtttatg agttcgacat aaggatacaa
     2401 ttcttttaaa tccctctcgt atttattgcc cagaaataga ctcagaattt taccaataat
     2461 actcatttta agctttctta ttttttatat gaatatcgtg caaagttagg tttattttct
     2521 taatggtgaa ctattgttta atcgcagtcg ttatttgtcg ggtgttcaga gttatttgat
     2581 tgaaaaagta tgaaatagca tcttttttat ccggcagtat gaaaaccagg agaacattga
     2641 aaatcgaaat ccctgtaaaa tttaacgata tattttgctt tttaactgga aataaaaaac
     2701 gctgttccga ttaaatttgc tttcctgcat caagcagctc ttcaattttt ttttcaagtt
     2761 ctcttaatga aaaaggcttg gtgaaaactt catcagctcc aagagttctg gctatgttaa
     2821 gatagccggc cggaccgaat ttgccaccac ccgagatagc tattatcttc agatcaggtt
     2881 ttaattcctt taatttcatt attaccttca gaccatcttc atcaggcata agaatgtctg
     2941 taattacaat atttatgaat gatgctttaa attttgccag tgcctcttta ccgttaacag
     3001 cttctataac tgaatatttt cttcttataa gggcatctct cagcatttct cttacgtcct
     3061 tctcatcttc aactattaat attccggcca tagaaaaaaa ttatttgttt aacgatattt
     3121 gaattgaggt aagaatttcg cgcaatgaaa ctggtttgaa aagaacattt ttagcgattc
     3181 cagaatttaa taatttttct tttgaaagat cataactgct gtcggtaatt agtacgacag
     3241 gtatgtcgat atttttttct atcatatatt ctgataatat atctgaactt atgttttcat
     3301 tatcctgcga aaatattatt acatcaggtt ttgcatcttc atcccctgac agttttatta
     3361 atccatcaat atctatagca taatcgacag catatccgct tttttcgagg gctgaaccga
     3421 gaacttttaa ttcatggctg ccgccgcaga taaaaagtac tctgcttctt tcggcaaaag
     3481 gtgctatttc ttccaggggc aaactatctg caaggggcaa atatatttta aactgtgagc
     3541 ctttgttttt aactgatttt acagttatct ccccatcaag ctctgtgatg ataccataag
     3601 caacagagag gccaagtcca gctcctttcc cgacatcttt tgtggtataa tatggttcaa
     3661 atattctgct tatcacagat tcctccattc ctgttcctgt atcttcaaaa cttatcactg
     3721 tatattcatc tgcgataata ctttttcccg actcactctg gaactgattt ccgttgattc
     3781 tttcctgtac aatagtaagg gttcctccgg tattttccat tgcttgaagt gcattcataa
     3841 tcaggttcat gaagaccctg aaaagttgcg ttggatcagc atagaccaga acatttctgt
     3901 ccctgaaatt tttttctatc gacacatttg gttttttaac tgattcagca aagcttactg
     3961 cttcatcaag taaatcgcaa atattgacag gtattttttc ctgacctacc tgtctgctga
     4021 atgtgagtat ctgattggta agttctctgg ccttagatac tgccctgatt atttttccga
     4081 ccatttcggt tccttcctgg ttatctttta tttcttcaag cagcatttca gagtaaccga
     4141 aaatagatga tagaatatta ttaaaatcat gagcaatacc tcctgcaagg gaacctattg
     4201 tctctatatt ttttgcctgg agcaaacggt gttcgagcat tatttctctg gttgaatcct
     4261 caaatgcaaa atcagcatat aatggatttt ttgtatcctt ttctgtaaca acatagatgt
     4321 ttactctgca tctgatttcg cggcctttaa aagttataag gggaactctt ttgaatgtac
     4381 tgatgcttcc atctgccgca tgcattatct ggacatcaaa aattgttttt tccctgtaaa
     4441 aatctttaat attcaaaccc tcaagatttt cattttcagt attttcaagt atatctgaaa
     4501 aagccttatt ataatgaata attgtaccat cgggcttaca tctcagaaga cctataggca
     4561 ggtgtgtctc tagtcttctg aattcttctt ccatttcact tattgctctc tgtgtaattt
     4621 tcagatctgt aatatcaacg gtttctgcca gcaggtgtgt aacatttcct tcttcatcag
     4681 gaagtggcct gaaaataacc tcaaagaatc tttctccata gagtggaatc tgaaagtttg
     4741 cctcatacct gacaatgttt ccattcaggc agatatcgat gttttctttt atattctctt
     4801 tgaatgtatc ttcaccccat aagtcagcca gggactttcc taaaatgttt tccggctgga
     4861 tattatggta tcgacagaat gtgttattga ccttctcgta gcaataattt cggttgataa
     4921 cactcaacat cgaccttgaa ttgtttataa ggtggtcgta aagatgcttg tcaatatagt
     4981 tcttttcaac agtcattcaa tatcatataa ccatttcact gatataaaat tactcaaaat
     5041 caagattgaa ttttttcctt atatccttga ttgcagggta ttttgaagtt agaaactggt
     5101 acttctgctc atctgaataa ataatatctt ctgattcaac cggatctaca acggtttcaa
     5161 tttcgaagaa atcttttccg taatattctt ccataaatgc aagcaatctt tgtttatagt
     5221 tccggttaaa caattccttt tgagcaatat tgttaagatg gatagtaacc agcttatctt
     5281 tatggacttc aacttttaga gatttgaaca tacccgacac cctgggtcct tcgtttttca
     5341 cagtctcagt gaattttttc cagagcttta aaaactcatc gggatcaaat ttttctttag
     5401 aagatgcaac tacaggcaat tcatcagttt tgatttctgt gggaaccttt tcttcagttt
     5461 gagtgtcatg gaaaccgttt atcagttcct taattgaaag tgatctttca ttacttacta
     5521 ttttattttc agggggtttt gattctctgg caatcagtat gtcactttta acttcctgtt
     5581 tttgagattg tttaggctcc tgtggttgca gggtatgaat atcttttttt tcaggtttca
     5641 gttggctctt ttttttttcg gtttcacctg gttcagtttc agaaatatta cagagtttaa
     5701 tcaatgccag ttcgacgtga aggcgctggt ttttactgct tttatatccg aggtcacaat
     5761 tgcttccaat ttccagtgcc ctgtagataa actcccttga acatttgcgt gactggctaa
     5821 gatatttttc ccttatcgca ggtgcggtgt caagaagttt aagagtaact tcatccttgc
     5881 atacaagaag gttacgaaaa tggctgttca gtccggcaat gaaattatga ccgtcgaagc
     5941 ctctggtaag aacctcatta aaggttagca gtatttttgc aacatcacca gcaagggcag
     6001 cgtcaacagt tctgaagtaa tattcataat ccagaacatt tagatgttca agaacagttt
     6061 cgtaagtaat cgaatttcca ctgtaactga ccagctggtc aaaaatagag agtgcatctc
     6121 gcattgaacc gtctgccttc attgctataa catgaagcgc attttcgtcg gcagaaacgg
     6181 attcattttt cgcaacgtat ttcagtcttt caacgatatc ttctaccctt attctctgga
     6241 agtcaaaaat ctgacatcgt gaaagtattg taggtatgat tttatgtttt tcggtagtgg
     6301 caagaataaa aatagcatga gatggtggtt cttcaagtgt tttcaggaaa gcattaaatg
     6361 ctgatgacga aagcatatga acttcatcaa ttatataaac actgaattta ccaatctgtg
     6421 gagggattct tacctgttct atcagggttc ttatatcttc tactttgttg ttggatgcag
     6481 cgtcaagttc atgaatgttg aacgacctca gagtgttaaa cgacacacac gattcacatt
     6541 tatcacatgc ttcgccgttt tcctgaatgt tgaagcaatt aattgtcttt gcaaatatcc
     6601 ttgcacatgt agttttccca acgcccctgg gtccgcagaa aagatatgcg tgagctattt
     6661 gtctgttttt tattgcattt ttaagagtac ttgtaatagg atcctgccct attaccatat
     6721 cgaaagttga tggtcggtat ttccttgccg aaacaataaa attctccatt aataattata
     6781 ttttatcaat tcaaccgggg ccagagcaaa tataattaaa aagagtgtca gtttttttat
     6841 tgttgaaaaa atcctttcaa atcctgagta tcattgataa atcctgaaaa aattgacata
     6901 taatattttt gtaaatttca aaattgttgt acttttgcga gccaattaac attaataata
     6961 aaattattaa aaattatgtt gaaccattac gaaaccgttt tcattgcaac tcccgtttta
     7021 tctgagaatc agatgaagga agcggtacag aaatttaaga aaattatcac cgaaaattca
     7081 ggtgaaatta ttcatgaaga gaattggggt cttaagaaac tggcttatcc aattaagaaa
     7141 aagtcaacag ggttttatta cctcattgag tttaaaggac ccggtgatat tgttgaaaag
     7201 cttgaactcc agtacagaag ggatgaaaga atcctcagat ttctcacctt caaaatggac
     7261 aagtatgcta tagagtatgc tgagaagaag agaaaacaga aagaagttga aaaaattacg
     7321 gaggaataaa agatggcaca gaacgaatca gaaataaggt atcttacgcc cccggcagtt
     7381 gagataaaga aaaagaaata ctgccgtttc aagaagaata gaataaaata tgttgattac
     7441 aaagaccctg aattccttaa aaagttttta aatgaacagg gcaaaattct tccacgcaga
     7501 attactggaa cttctctcaa gtatcagaga agggtggcaa aagctgtaaa aagagcaagg
     7561 catctcgcac tgctcccata tgttactgac ttgttaaaat aaggaggttg agatggaagt
     7621 tattttattg caggacgttc cgcagcttgg acataaagac gaaatagtaa aggtaaagga
     7681 cggctatgga cgtaattatc tgattccgaa aggtatggca atagctgcca ctccatcggc
     7741 aaagaaagtc catgctgaaa acctgaaaca aagagctcac aaagaggaga aaatacgtct
     7801 tgaagcacag gcacttgccg agaaacttca aggaattaac ctggttatag gggcaaaaac
     7861 aagcagcacc ggaaaaattt tcggttcagt aaatgcaatt cagattgctg aagctcttaa
     7921 agaaaaaggg tttgaaattg accgcaagaa cattatgctt accgcagacc agataaaaga
     7981 agtgggtaag tataaagcca caataaagct tcaccgtgac gtgaaggctg aaatcgaatt
     8041 tgaagtagtt tcagtctgaa tactgtaacg taaactattt cttcgcagct attgcatcta
     8101 tttctatcag ggctcccatc ggcagatctc tgacagcaaa agccattctg gcagggggat
     8161 ccgatgggaa gtattccata tagatgcggt tcatgggagc ataattgttt atgtcagtca
     8221 atagaactgt gcatcttaca acatcttcaa gtttatatcc tgccttttct attacagttt
     8281 tcagatttga aagtgtttga cgtgcctgtt cctcaaaccc tccctcaatc aatttacctg
     8341 ttgcggggtc aagtgctatc tgtcccgaga catagagcat atttccaact tcaacagcag
     8401 ggctataggg cctggaaacc ggtgtatttt caagtgttat gaccctgcgg agaggtgaat
     8461 cctgtttttt tacctgtgtg ttgcaggaac tgaaaaatac agccgaaacc aatatccaaa
     8521 caaataatct gttcatagat aattattttg attttattgc aattgcttca atttcaacaa
     8581 gtgctcctaa cgggaggtct ttaaccgcta aagctactct tgccggttgt tctgacggat
     8641 agaattttga atagacctca ttcatggttt taaaatcttg aatattactg agaaagacag
     8701 ttgatttcac aacatccctg taagtgtaac ctgctgcttc gagtattgcg ccaatatttc
     8761 tgagtacctg ttctgtttgt tctcgtatat caccttctat aagcttgcct gtagcagggt
     8821 cgagtgccac ctggcctgag acaaagagta ttccaccagc ttcaactgcc tggctgtaag
     8881 gtccggctgc taccggagcg ttacttgtac agattattct tttcatagtg aattattttt
     8941 gatgaccaat acgatatacc atattaataa aactgatccc tgaaatcttt tcttctttca
     9001 tacttcagat cggcaagcac tgaggctttt acttttattg tgaaatacca gctcttcatt
     9061 gtcccgacag gtatccaatc gaagctcata tcccagcaat gcaaatcgcg gtaaatacct
     9121 attcttgtcg tggttatctg tttgtttttg aaatcaaaac ctgtttcgta ggttatcttt
     9181 gttttcttag taagagataa atcacccgta agagataagg actggataac tgatgaagta
     9241 agtgcctgtt ttgaataatt catgctgtaa gccagatgca tagaccatgg catgtcgaac
     9301 ctcggatagt cagaatctga ttctctttca tcttgatatg ctctttgagc ggatggtcca
     9361 ctcaccggtt cttcttcacc atatccacct ctgcttacca ttcctggcgg cgtatttgtt
     9421 gcacctgtct ttttcctgct gaagagttga tttacatcaa agtcaacact gacattaaag
     9481 ccggtaagcc ttaatggttt acccgtttgt ttgaaataaa atttatttat tacctgaccg
     9541 ttttcattgg ttccatagag tgaaaaagag ctgtttgcgg ataatccaat attatttaac
     9601 agtactgtgc ggaaacttac accaactggt gaccacctga ggctatctgc aaaaatatta
     9661 tagtaagtac ctgcactgag gttttcaatt atttttactt ttttagcaac acctgttgtg
     9721 tcatttttat catgaatttt agcttcaact atatttacaa ggctgaaact tatcccaccg
     9781 cttcttttac ctgcagaggg tgtaccatgg atattccctt cgaatattga atatgttctg
     9841 gtgacaccgt ttacatcggt atattgcctg tacatgttgg ttgtaagacc tttaattaca
     9901 ggaacatagc taaatgaaat agaagatttt ataacatgcc tgattgcctc gagcctggag
     9961 cctggtttaa actggtacat tccaaacaac tgaggattaa taccagtgct gattgaggtg
    10021 ttaactgatt gcccgtaaaa aaatcccctc aatgtatcag taataacaac aggtcttgtc
    10081 ttacccagtt ccggatcgaa gtaatctgtt accaaacgtt tgttatattt ctgtgtataa
    10141 agcactccgg agtatgataa ctggggagaa ataataaaac ccgggatttt tttgaaaggt
    10201 ctgatctgaa ggctcaaagg tgcgtcgtgc ctgaaaccac tctgcatgtt attgaataac
    10261 gacctggtaa acagcaaaga atcggcaatg ttatatattc ggttgtccag cttggcggta
    10321 tactgaaact gcaggtcttc gtaccagcgg gatggtccaa ctcttttcct gtttttaaag
    10381 gggtatattc tcgacataga aaatgtggcc tggggaaggt taaggctcat tgttttattc
    10441 tgaacgttct gactgtggtt taaacttgca gtaaaattaa aaggtgttcc ctcccatgtt
    10501 tttgaatagc ttatactcga ttgcctggtt gtatttacat gctctgctat cacataactg
    10561 ttttgcctgt catataagct tgaactcatg ctaacacttg ctgaaaatct tgaacctgga
    10621 ctggcttttg ggtcctggct gtaagtccag tcaattctgt agttcttagt ttttgaataa
    10681 tctggcagtc ccatatggcc ggtaacattg ttggcataac ttaaaaataa acggcctgaa
    10741 tatttatacc tcttgaaata gttagatgag gcactaacca tccatgtacc gttagtgtac
    10801 agtgtcccgg tgagagaaag gtcaatatta tcatttatgg caaagtaata acctccatcc
    10861 cgcagtgaat agccaagttc ataagtagtt ccaattttag gcataataat tccggaagct
    10921 gctgcattct tacgatagag agggaaatag ccaaagggta ttacaacagg caggggaatt
    10981 ccttcaagga caagatatgc agggccggta actatctttt tcgcaggcac cacctttgcc
    11041 ttattaaatc cgatgtaaaa atggggagga tcagcatcac atgttgaata tgtacttctt
    11101 ccaatattta gacttccatc gtccagtcgt tttgtaactt tacttctaag atatccttct
    11161 tcctgttgcg taaccaggtt aaagactctt cccttccttg ttctgaaatt atattccaat
    11221 gcttccgact cgaaagattc ctcaccctgt ttaaagaccg gacgtccaac catcttaccg
    11281 gttgaatcgg gtctgcctgt tgcgtaaata attcctgttt cagtgttcag tacaatcgaa
    11341 tcggctgtaa gtgttatatc tccatatgta accacggctt cttcgaccag gtatactttc
    11401 ctgttaacca tgtctgtacg tctgtaaccc tttgctgtgt gggttattgt gcggtcaaca
    11461 gcatcggggg aaattttaag aggcttcaga atagtatctg ttaaaatact atctttcagt
    11521 acaaaaagtg tatcaggtaa tgcctttccc tcctcctgcg aaaacacagc aagaaacctg
    11581 aataaaatca gaaaaaagaa tatgaaccgt tttattttaa aaaaataatg caactttaaa
    11641 ttttattttt gcgacatgtt ggagatattt ccggcaaaac taacacaatt taatgtacaa
    11701 taaatggtcg taacaattaa aaacggcgta ctcaactatt ctattatctg cttttacctg
    11761 gttttgttat tgttatcaat ttgtaattca gtttatgcgg gttctggtta tcagcaacca
    11821 gtcaaggaaa aatggatcat tgttatagat gccggacatg gtggcaggga cccgggtgca
    11881 attggttctt tcagctacga aaaggacata acacttgcaa tagctttaaa gacaggcgaa
    11941 ctgatagaaa agaatatcag taatgccgaa gtgatataca caagaaaaac agatgttttt
    12001 atggatcttg ccgagagggc aaatctggca aataaaaaca aggctgattt atttatttcg
    12061 attcatgcca actggcacag atacagaaat acttatggtg cagaaacata tgttatgggg
    12121 catcacaaag accagcagaa tcttgaagtg gcaatgaagg aaaacgaagt tatacttctt
    12181 gaaaaagatt acactaccaa atatgaagga tttgatccaa aatcgcctga atcatacata
    12241 atatttacat taatgcaatc tgcctttttt gagcagagca cagatcttgc gtcgaagatt
    12301 caggttcagt tcagggagca gattaaaaga ttcgatagag gtgtaaaaca ggcaggtttc
    12361 tgggtgttat atatgactac aatgccgagt gtactgatag aaacaggatt catttcgaac
    12421 tctgcagaag agaagtatct gaattcaaaa aagggacagg aagaaattgc agaagcaatt
    12481 ttcagagcat gcagtgatta catagctgaa gtctacaaaa aaagccaaat ctcagcaatc
    12541 aggattgata acaatgccag ttcagaggga aatgcaggtg caccgcaaag caatattgtt
    12601 tttatggttc aggttctttc atcaacaaga aaaacagaaa tcactccaaa aaattttaaa
    12661 aacctgactg acataacaga aatccatgat ggtaaccgat ataaatatgc tgcaggaaaa
    12721 ttttccgact ataatcaggc agttgcttac aggcgtaaaa tagaatcact ttttccagat
    12781 gcatttgtaa ttgctgttaa agaaaacaaa atattacctt tgcaggatgc tctcaatgag
    12841 ttgaagaaaa aataacggag aagctatgaa atttaaaata tcaaatgaat taaaggttgg
    12901 ggtaattgca ataattacga tactagcatt catctatctc tataattatc ttaagggaaa
    12961 ggatcttttt agtaccatgg ccagttatta tgtcatttat gatgacataa gcgggttaac
    13021 tgaatcgagc cctgtggaaa ttaatggttt caaggctggt gttgttcaga ccattaatct
    13081 tatgaatgat aatactggca gactgcttgt agaattatca ataaagaaaa attatgctat
    13141 tcctcgagga agtgtggcag aaattacgag tgtatcgcta atagcgggaa tgaaagtcag
    13201 gttgctttac gggcctggtc cagaagttta caggaatggt gatactattc caggcaggtt
    13261 atctcctccg attcttacca cgtttgaaaa agatttctct gtaatcaggg aaaaggttaa
    13321 caacctgatt gatgaactcg acacagcaat ttcgggaatt aatgacttat tgtctcctga
    13381 attcaggtcc aatttaaagg gtagcatctc aaacataaac caaacaactg caagtttgag
    13441 tgaaataatg ggcacaaaaa agactgagtt gaaagatgct gttgacaatt tcactgcttt
    13501 ttcatctatg cttgcatcaa gttcaggcaa gattgacaga actgtcagta accttcaggc
    13561 gataactgat tctatttcgg cttcaggctt atacgaatct cttaccagtc tgaaaatgac
    13621 acttgaagaa accagaactc ttcttgatgg cataaacaaa gggaaaggca cagcaggtca
    13681 gttggttaac aatgacactc tatatagaaa tttatcatcc agtcttgaaa gtcttgactt
    13741 gttattaaaa gatttaaaag aaaaccctaa aaaatatgtt catttttcaa ttttcgggcg
    13801 gaaatgaggt caattaataa aaattattta aatatctctt tatcacagct ttatttccat
    13861 cgaaaatagt actccaaaat agtactagtt ttttccttga aatattattt ttttgattaa
    13921 ttttttaatg tattgtaaca catacaagaa tgcgaaaaaa attttataac tatttgattt
    13981 attgttattt accaataatg aaaaaatttt tcaattataa gttattgatg attaaattca
    14041 tataattttt tttaaaaaat caagcaaaaa aaattttttt ttttagtttt ttttttgca
//
LOCUS       DAOSNB010000028        14002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4377_length_14002_cov_4.071700, whole genome shotgun sequence.
ACCESSION   DAOSNB010000028 DAOSNB010000000
VERSION     DAOSNB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14002
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_14002_cov_4.071700"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PLG42_02355"
     CDS             <1..1260
                     /locus_tag="PLG42_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU30084.1"
                     /translation="WFSSWLWPITCFDGINNPGNPDMKYYYPTSDLVTAPEILFFWVA
                     RMIIAGYEFTGKKPFSNVYLTGIVRDQQRRKMSKSLGNSPEPLELIEKYGADGLRVGM
                     LLCSPAGNDLLYNDSLPEQGRNFANKIWNAFRLVKSWKIDEKIAQPEASALAVEWMEE
                     MFNRTLGEIDSDFRKYRISEALMKTYKLFWDEFSGWYLEIIKPEYQKPVDLKTYNATI
                     SIFERLMQVIHPFMPFITEEIWHLISERKEGESIMVSRMPVHKKHHDRFIHRFEVAKE
                     IISAIRAIRKEKNIPQADKLDLYIKTDNSYDGYYLPVIRKMCNLNEIKYTEEKVQDAI
                     SFLVRTIEYYIPVRTKVDTAEELKRTREELEYTKGFLAIVMKKLDNEKFIRSAPSDVV
                     ERERKKQADALVKIKALEEKIKELENR"
     gene            complement(1264..2574)
                     /locus_tag="PLG42_02360"
     CDS             complement(1264..2574)
                     /locus_tag="PLG42_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30085.1"
                     /translation="MKKWFFIQIIILAASCSKPDNNPDIYFNAEKALAYFRALDSICE
                     ADRGRLWGENLAGPVVFVDRPTRRLFANKPDNQGQLKLKDGVYQGIFPRERLIENSAV
                     DIGGKTYAMVPLPPREDPYRIKTSAINALFHCLQKNHGITLERFAFRSMDDRFARIWL
                     KLEFRALKKTLEADSNSFVNYLRDAIIFRGARRELFPSSIKEENRFEIYEGLSMLTSI
                     LLCANSPEEAKQRFLENLEIVYRFPSFSRAYGSIIGSTYSYLLYTGGFDIKPLLISDT
                     ADLGKIAIEYFNLQTPAIYRDVAGSLALVYDVDNIYKEEEQRMNEIRERIRRQLSRFT
                     EKPVVTIELESPYFDFEPEDIRSLDTLGTIYNSIRVADNWGKLTVDKGGCLVSYNLKY
                     IRLPAKNIKETKNHYYGDGWHIIMNNNWKIVKVNDNYILRKIAP"
     gene            complement(2670..3527)
                     /gene="nadC"
                     /locus_tag="PLG42_02365"
     CDS             complement(2670..3527)
                     /gene="nadC"
                     /locus_tag="PLG42_02365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOU30086.1"
                     /translation="MNQNDILKDFILRCLDEDIGEGDVSSSACISPEEQGRARLIVKE
                     NCVIAGLAVVRRIYSIVDESLSLDLFINDGDEVQKGTIGFIVSGNIHSILKSERLVLN
                     ILQRMSGIATVTRAYVKRLEGLKTKVTDTRKTTPGMRFLEKEAVRIGGGENHRMGLYD
                     MIMLKDNHIDYAGGIENAIARTKDFIKRTGKNIKIEIEARNLDEVKKILATGGVDRIM
                     LDNFSVEETRKAVELIAGRYETESSGGINLDNVRSYAECGVDYVSVGALTHHLKSIDM
                     SLKALTSQQ"
     gene            complement(3514..3948)
                     /locus_tag="PLG42_02370"
     CDS             complement(3514..3948)
                     /locus_tag="PLG42_02370"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOU30087.1"
                     /translation="MKGKNYNINEMYKKLFFSSFFLVLTLAISMAQEQTRIPPEIITA
                     FRTGNAADMSRYLNSTCQIMLQGKEDFYKKNVAETILKDFFSTYRPREFIVKHQGGTS
                     DAQYAIGNLRTEKNNFRVYFLLKKVGSDFLIHQIRIDPDESK"
     gene            4075..4548
                     /locus_tag="PLG42_02375"
     CDS             4075..4548
                     /locus_tag="PLG42_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOU30088.1"
                     /translation="MKIVLLQTGKTADKYISEGVADYSARITRMTGFEIITVPDLKNT
                     GKMPPAEQTLREGRAILNCIRNDDYVVLLDRKGKALDTIGFASELQKFLLMPKKRVLF
                     LIGGAWGVSEEVYKRANFILSLSKMTFSHQMVRLLFIEQLYRALTIIKGIPYHHE"
     gene            4573..8280
                     /locus_tag="PLG42_02380"
     CDS             4573..8280
                     /locus_tag="PLG42_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU30089.1"
                     /translation="MKFKMKITGFLVAAIVSTAFAFLAESVYKSDLEYRIRTKRFNKI
                     LVAKESIAEECLGNLKNILEKGEEFGSISNSGINKIIKEEGITILEYLDGKLVHWTDN
                     SFEVPVVYDDSLFSKRFVFIHNGWFIPKKINAGHEIIITLLRIRSEYGFENDLVRNGF
                     VTAFRVPEGTGLSFDEDSKYPVYNKEGIKLFNLVWPDKKGITLFAVFPVSMWLISFLL
                     IILIVLKTAEIIALKGRGLFAFLFSIVFFTVLYLIILFFIRYSVFEKTRLFSGYLFSY
                     GKFIPSLGHLMVLSILLSVSANIFYRFIQLKFSEIKSKTGFIAALSISFIIGVLILSA
                     CHIILTELIFDSNIVFEPYRVLELTPDTLAGYITVLILMVTPVLFMMKVIHEAVDFAS
                     NKVLFVLFAVALIFMGVIAAVLKTDISMLVVFYIALVSAICYSLKKKTITFNLTVFVS
                     VFFALYALYYVIKLSEKKSMEQRKVLAVAYSTEHDPVAEHLLIDLWPSLSSDTILKKM
                     VCREFATQNEIDTITYYLHNNYFGGYWGNFFLSVVSCRHDSPLWISSEEKMIDKCFDF
                     FRERIEKYGHQITGTEFYFLEGQGGRSYYLGNIFYDLADGGRKGLFIELYNDIGSFQE
                     GYSELLLDKKYQGYYRLKYYSFAKYINGNRVLRTGDFPYEKTDDAYIDREQDFRVFNR
                     EGYKHLLYRSGNVTVMISSKRISFTDVLISFAYIFAFTLLCLNIVLFLVRRHSLKSLI
                     YLNFRQKLQVSFISVLLLSFVCVGVIVAVFSVNQYKNRHLETIKEKINSIYTELDDRL
                     AEEPDLAADWRDAANPSLNEFLIKLSNVFNTDINLYDRNGYLMATSRPEVFYRNLTSR
                     RLNMDAFINLENLTKSEYIQREKIVSLEYLSAYVPFYNEQGYLLAYLNLPYFRMESVL
                     ARQISNVIVAVVNFTLLIIIITMVIAVLISRRLTAPLRMLSEGLASVQLGKKSEHLVY
                     RGHDEIGEMVSQYNRMVDELQESARKLADSEREYAWREMAKQVAHEIKNPLTPMKLNV
                     QQLYKSWKDGTPGFEKKLEKFTKNQIEYIETLSSIASAFSSFAKMPSANPMDLDLVEQ
                     LRTTLELFKDTENISFRIDWPVDTKIFIYADKEHIISIFSNLIKNAIQSIPQGRSGLI
                     KIGLELNGDRAIVSVADNGTGIPEDLKPKLFTPNFTTKSSGMGLGLSIVKRYVETAGG
                     RVWFESEKDSGSVFFVELPVKFTVEKSGQAK"
     gene            8393..12559
                     /locus_tag="PLG42_02385"
     CDS             8393..12559
                     /locus_tag="PLG42_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU30090.1"
                     /translation="MLILSISAPAQKNLSGVLGMPAAHVVSIGVDRVTVDDVTGFNIA
                     GGDTVLVIQMQGVMILDDPGSYGQLQDTIGRPGLWEFLITQSVNTGTKEIIFRNELKN
                     NYGTEGNVQVVRVPFYNSVDVTGTLTVNGWDPVKKTGGVLAMIVGRTLKLSADIDVTG
                     KGFTGGKDTIGDGICRATDETTYGKPFYPRTFTNAGYKGEGIANYTKDGTLLVPDYLK
                     GYGPNYTGGGGGNGRFAGGGGASLIGAGGTGGFEDFFCSPNPGEGGTGGIKADHPSIS
                     GGLFLGGGGGSSTRASTGGTAGPGGNGGGIVIIVADSIIGNGGNIRASGAPGGNASGS
                     GGAGGGGAGGSIALSVKNYGTSAMSLNVSGGKGGDRLPGNGGEGGGGGGGLIWVSTAM
                     PANISTDLSGGEAGMSSTPLASPGQPGEKKTDFKAALNGFLFNSIRSSITGNQVDSVC
                     SNMLPPLITGTTPVGGVEPYTYTWEKSYDQSTWIQVASGTDASYINYTPTVVETSTVW
                     FRRIITDSSSPLPLIDVSKPVQIIVQPYIKNNIIGTSDTICFAQDPPTFTSKATLQDG
                     NGIYYFRWEASTDSNKFILPVNTYNEENYTPPPALKQTSWYRRTVISGRCIDSSAVVK
                     ITVLDTIKNNTILTPAEEICYGMLFTDISATTPPVLAGGDGIYRYKWESSPDGTSWSN
                     AEGTVNNSDYNPVESSSSFPGTLYFRRVVYSGSNDVCVNHSAPVQLTAWPVIGNNTIS
                     ADQTICSGSIPAALTGTLPVNGNGTYSYLWQNKTKSSSWVTAAGPNPFNQQNYMPPAL
                     TDSTWYRRVVTSSACSDTSNVIVVNVHKPISNNNIQLLSGLTDTTICSGGKLNLFIGS
                     TPAGGTDLPGDYLYQWETSPTAVGGYVPITGATSKDFQSDVIINPTSLPVDHYFRRFV
                     SSGTCSAPSSQIIKVTVLPSITNNVITPDKTAVCYNTSPVISGTPLTGGAGGTPTWLW
                     IESPDGITSWSPASGTNNQQNYTPPPLTSPVFYRRVILSGPANCCIDTSNVAGISINP
                     LPEGYITSITDTTVCSGIQVPVKIHLTGASKWNLTYTENGTPVVLNKIMNQDTVIIIN
                     KTPSGDVEIYNYALQGLVDANGCNATVLTGSRKITVYRTPVANAGADEAVCGPVYTLK
                     ATPSVGTGSWLLPSDVTNLTAPGPSMTVAIDSLNAPAMLKRKFYWEEINWTCTDKDSV
                     EITFYKRTGQANAGPDKDLYSFEKVDTLHAAKPLVGTGTWSVLSGSAQIVKDSIVINL
                     SPGVNRFEWKVVNGLCESKDEMVINVHELVVPEGFSPNGDGINDEFVILGLDLNYNEC
                     SLRVLNSAGSEVFFTTNANGRTWEHWNGESGGNPLPEGTYYYMLTIKSKRTDSSFKKS
                     GFVVLKRYNY"
     gene            12597..13559
                     /locus_tag="PLG42_02390"
     CDS             12597..13559
                     /locus_tag="PLG42_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU30091.1"
                     /translation="MKGRLFLLMLIMSCLHLRGQDGTSDMTKISFFYPVYSQYLHNGL
                     VINPAYAGTREALSFFVSGRMQWMGIEGAPVFQTASLHTLLKNNKVGLGLSGQFFRYG
                     YTRATSVYTDYAYHIVIGKSRLSMGLRAGFDMSNTDYTGIILIDPNDPVFITNDKPYM
                     LPNVGAGIYFSNKRFFIGASVPSFLTYLKSSSGEISYNTFREFDANLTAGALISFSNS
                     FKFKPSVLVGYPFREKTNNDEIRIDLNGNFIFFDFLWLGASWRTYEDVIVGIIQLQVT
                     PQLMFGYSYDYPSGQMSTYSKGSHEVILRYEFGYKVSATNPRYF"
     gene            13563..>14002
                     /locus_tag="PLG42_02395"
     CDS             13563..>14002
                     /locus_tag="PLG42_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30092.1"
                     /translation="MERWWIHIVIFSLCLICGRDYLFSQPLVYKIEKMPFSSSQFNDI
                     APVIIKDGIIFCSDRRASSAKNVTTFNEERLYNLYFSARRDTLKWREPYEIKISGDPV
                     LFTGPVSVSADGSTIYFTSSVLSGKAARKRNIKNTLGIYVGELSG"
BASE COUNT         4114 a         2645 c         3054 g         4189 t
ORIGIN      
        1 tggttctcat cgtggctgtg gccgataaca tgcttcgacg gaataaataa ccccggcaac
       61 cccgacatga aatattatta cccgacgagc gaccttgtaa ctgcacccga gatactattc
      121 ttctgggttg ccagaatgat tattgccgga tacgaattta caggaaaaaa acctttcagc
      181 aatgtgtatc taacaggcat tgtgagagat cagcagcgca ggaaaatgtc gaagtcgctg
      241 ggtaattcgc ctgaacctct tgagctaatc gaaaaatacg gtgcagatgg tttaagggtc
      301 ggtatgttgc tttgctcccc agccggaaat gatctcctgt ataacgatag cttgcctgaa
      361 cagggaagaa attttgcaaa taagatctgg aatgctttca gacttgtgaa aagctggaag
      421 attgacgaaa aaatagcaca gcccgaagct tcagccctgg cagtggaatg gatggaggaa
      481 atgtttaacc gtactctggg agaaattgac tccgacttcc gaaaatacag gatatcagaa
      541 gctctgatga aaacctacaa gctcttctgg gatgaatttt cgggttggta tctcgaaatt
      601 ataaaacctg aatatcagaa acctgtggat cttaaaacat ataatgccac aatttcaatt
      661 tttgaaagac tgatgcaggt tattcatcct ttcatgccat tcattacaga ggagatatgg
      721 cacctgattt ctgaaagaaa agaaggcgaa agtattatgg ttagcaggat gccggtccat
      781 aagaaacatc acgaccgatt cattcacagg tttgaagtgg ctaaggaaat aataagtgcc
      841 atcagggcaa tacgaaagga gaaaaacatt cctcaggctg ataagcttga cctgtatata
      901 aaaactgaca acagttacga tggctactat cttccggtta taagaaaaat gtgcaacctg
      961 aatgaaataa aatatactga agagaaagta caggatgcca tatcattttt ggtgaggaca
     1021 attgaatatt atattcctgt caggacgaaa gttgatactg cagaggaact caaaaggacc
     1081 agagaagagc ttgaatatac gaaaggtttt cttgccattg tgatgaagaa gcttgataat
     1141 gagaagttta tcaggagtgc accgtcggat gtggttgaac gtgagagaaa gaaacaggca
     1201 gacgcccttg taaagattaa agcactggaa gaaaagataa aggaactgga aaacagataa
     1261 ttttcagggg gcaattttac gcagaatgta gttatcgttt acttttacaa tcttccagtt
     1321 gttattcatt attatatgcc acccatcgcc gtaatagtga ttttttgtct ctttaatatt
     1381 ttttgcaggt agtctgatat atttaaggtt ataggaaaca aggcaacccc ctttatcgac
     1441 agtgagtttt ccccagttgt ctgcaaccct tattgaatta tatatagttc ccagggtatc
     1501 gagcgatctg atatcttccg gttcaaaatc aaaatagggg ctttcaagct caattgtaac
     1561 aactggtttt tcagtgaacc tcgatagttg cctgcggatc ctctccctta tttcattcat
     1621 cctctgttct tcttctttat agatattgtc cacatcatat accagtgcaa ggcttcctgc
     1681 gacatcccgg taaatagcag gtgtctgaag attgaaatac tctattgcaa tttttccgag
     1741 atcggctgta tctgaaatca gaagtggttt aatgtcgaaa ccaccggtgt agagaagata
     1801 gctgtaagtg ctacctatga ttgaaccata tgctctcgaa aatgacggaa atctgtatac
     1861 tatttctaaa ttttccagaa atctttgttt tgcctcttcc ggcgagtttg cacagagcaa
     1921 tattgaagta agcattgaga gtccttcgta aatttcaaac ctgttttctt ctttaattga
     1981 ggaagggaat aattctcttc tggcccctct gaagataata gcgtctctaa ggtaattgac
     2041 aaaactattg ctgtcggctt cgagggtttt tttcagggcc ctgaattcca gtttcagcca
     2101 gatacgcgca aacctgtcgt ccattgagcg gaaggcaaaa cgttcaagag ttattccatg
     2161 atttttctgc aggcaatgaa aaagggcatt aatggctgac gttttaatcc ggtatggatc
     2221 ttcgcggggt ggaagcggaa ccattgcata agtttttccg cctatatcca ctgcagaatt
     2281 ctctattaat ctttctctgg ggaaaattcc ctggtagact ccgtctttta atttcagctg
     2341 gccttggtta tcaggtttgt ttgcaaacaa tcttcttgtt ggacggtcga caaatactac
     2401 cggacctgca agattttcgc cccataacct tcctctgtct gcttcgcata ttgaatctaa
     2461 tgccctgaag tatgccagag ccttttcggc attgaaatat atatctggat tgttatcagg
     2521 tttggaacaa cttgctgcca ggataataat ctgaataaaa aaccattttt tcatgcctga
     2581 tttttagata gccaaaagta attaaatttt gttaaagtgt taataattcc taaatgggct
     2641 ttagaaaaac cattatttaa tttaaaggtc tattgttgac ttgttaaagc tttgaggctc
     2701 atatcgatgc ttttgaggtg atgtgtcagg gctcccactg aaacgtaatc aactccgcat
     2761 tcagcataac tgcggacatt atcaaggttt attccacccg atgactctgt ctcatatctt
     2821 cctgcaataa gttcaacagc ttttcttgtt tcttcaacgc tgaaattgtc cagcattata
     2881 cggtcaacac ccccggttgc gagaattttc tttacctcat ctaaatttct tgcctcgatt
     2941 tcaattttta tgtttttccc tgtccgtttt atgaaatctt ttgttctggc gatagcattt
     3001 tctatgcctc ctgcataatc aatatggttg tctttgagca ttatcatgtc gtaaagaccc
     3061 attctgtggt tttctccacc gccaatcctt actgcttcct tttcaagaaa cctcattcca
     3121 ggtgttgttt tgcgggtgtc agtcactttt gttttgagac cttcaagccg tttgacataa
     3181 gccctcgtta cagtggcaat tccgctcatc ctctggagaa tattcagcac aagtctttcg
     3241 gattttagaa ttgaatggat atttccggaa acaataaatc ctatcgtgcc tttctgaact
     3301 tcgtcgccgt cgttaataaa gagatccaga cttagtgatt cgtcaactat cgaatatatt
     3361 ctgcgcacaa cagccagtcc tgctatcacg cagttttctt tcacaatgag tcttgcacgg
     3421 ccttgttctt caggagaaat gcacgcagat gatgacacat ctccctctcc aatatcttca
     3481 tcaaggcagc gtaaaataaa atcttttaaa atgtcatttt gattcatcag gatcaatgcg
     3541 tatctgatgt ataagaaaat cagagcctac cttcttgaga agaaaataaa ccctgaaatt
     3601 gtttttttct gtcctcagat tacctattgc atactgtgca tcacttgtac ctccctggtg
     3661 tttaacaata aattcccttg gacggtatgt tgagaaaaaa tctttcaata tggtttctgc
     3721 aacatttttt ttgtagaaat cttccttgcc ctgtagcatt atctggcatg tagaattcaa
     3781 atatcttgac atgtcggctg cattgcccgt tctgaaagcc gtgattattt ccgggggtat
     3841 cctcgtctgc tcctgtgcca tagaaatggc cagagtcaga accagaaaaa atgatgaaaa
     3901 aaataacttt ttatacatct cgttgatatt gtaattttta cctttcactt cagaatatgc
     3961 aaatttaaac aaattctttt atcaaaacct tttaaatgaa tactgattac cggttctgtg
     4021 caccggtatt attgttattt ttgttttgca tagcaaaaat tgtgctgaat tctgatgaag
     4081 attgtattat tgcaaacagg caaaacagct gataaatata tttccgaagg tgtagcggat
     4141 tattcggcgc gtatcaccag gatgacaggt tttgaaatta ttactgtgcc cgacctgaag
     4201 aataccggga aaatgccacc cgcagagcag acactgaggg aaggcagggc aatactcaat
     4261 tgtatccgga acgatgatta tgtagtattg ctcgacagaa aaggcaaagc tcttgatacg
     4321 attggatttg caagcgaact tcagaagttt ttacttatgc ctaagaaaag agtgctcttt
     4381 ctgattggtg gagcgtgggg tgtttcggag gaggtgtata aaagagcaaa ttttattctg
     4441 tcgttgtcaa aaatgacttt ttcccaccag atggtacggt tattgtttat tgaacaacta
     4501 tatagagcgt tgactataat taaaggaatt ccatatcatc atgaatgatg ctgctattaa
     4561 ttgcactgaa ttatgaaatt taagatgaag atcaccggct ttctggttgc agcaatagtt
     4621 tcaactgcct ttgcctttct ggcagagtcg gtttacaaaa gcgacctgga ataccggatc
     4681 aggacgaaac gtttcaataa aatccttgtt gccaaagagt caattgcaga ggagtgcctt
     4741 ggtaacctga agaatattct tgaaaaagga gaggaattcg gttcaatttc gaacagcggc
     4801 atcaataaga taataaaaga ggaaggaatt accatacttg aatatcttga cgggaaactt
     4861 gtccactgga ccgacaatag ctttgaagta cctgttgtat atgatgattc ccttttctca
     4921 aagagatttg tcttcatcca caacgggtgg tttattccga aaaaaattaa tgcaggacat
     4981 gagataataa ttactcttct gagaataaga agtgaatatg gttttgagaa tgacctcgtc
     5041 cgtaacggtt ttgtgacggc tttcagggtg cctgaaggaa caggcttatc ctttgatgaa
     5101 gacagcaaat accctgtata taacaaagag ggaattaaac tctttaacct tgtatggcct
     5161 gacaaaaagg gaataacact gtttgccgtt ttcccggtca gcatgtggct gatttcattt
     5221 ttacttataa ttctgattgt actcaaaact gcagaaataa ttgctttaaa aggaaggggc
     5281 ctcttcgctt tcctgttttc gattgtattt tttactgttc tatatttaat tatattgttt
     5341 tttatcaggt attctgtatt tgagaaaaca aggctatttt cgggatatct cttctcatac
     5401 ggtaaattta ttccttccct gggacatctg atggttttga gtatattact ttcagtatcc
     5461 gccaacatat tctacagatt cattcagctg aagttttctg agattaaaag taaaacggga
     5521 tttattgccg ccctctctat ttcctttatt atcggagtat taattctttc agcctgtcat
     5581 attattttga ctgagcttat atttgattcc aacatagttt ttgaacctta cagggtactg
     5641 gagctaacgc ctgatacgct cgcgggttat atcacagttt tgatactgat ggtaaccccg
     5701 gtccttttta tgatgaaagt tattcacgaa gcagtagatt ttgcatcaaa taaggttctt
     5761 ttcgttctgt ttgctgttgc attgattttt atgggagtaa tagctgcagt gttgaaaact
     5821 gacatatcaa tgctcgttgt tttttatata gcactggttt cagcaatatg ttactctctg
     5881 aagaagaaaa caataacttt taaccttaca gtatttgtat cggtattttt tgccctttat
     5941 gctctttatt acgtaataaa gctctcagag aagaaatcca tggagcagag gaaggtgctg
     6001 gctgttgctt attcaaccga gcacgaccct gtagcagaac atttgctaat tgacttgtgg
     6061 ccatcacttt catccgatac aattctaaag aaaatggtat gcagggagtt tgctactcaa
     6121 aatgaaatag atactataac atattatttg cataataatt atttcggagg ttactggggt
     6181 aattttttcc tttcagtggt atcttgccgt cacgattctc ctctgtggat atcttcagaa
     6241 gagaagatga ttgacaagtg ttttgatttt ttcagggaac ggattgaaaa atacggacat
     6301 caaattacgg gaacagaatt ttattttctg gaggggcagg gaggaagatc gtactatctt
     6361 ggaaatattt tctatgacct tgctgatgga ggcagaaaag gactatttat cgaactttat
     6421 aacgatatag gttccttcca ggaaggatat tctgaactgc ttcttgataa aaaatatcag
     6481 ggctattacc gtcttaagta ttattcattt gcaaagtata taaacggaaa cagggtctta
     6541 agaaccggtg attttcctta tgaaaaaact gatgatgcct atatagacag ggagcaggat
     6601 ttcagggtat tcaacaggga aggctacaag catcttcttt accggagtgg aaatgtaact
     6661 gtaatgatca gcagtaaaag aatctctttt acagatgtac tgatatcatt tgcctacatt
     6721 tttgccttta ccctactgtg tcttaatatt gttctgtttc tggtaagaag acattcactg
     6781 aaaagtctta tttatttgaa tttcaggcag aagcttcagg tttcatttat ttcggttttg
     6841 cttttgtcgt ttgtttgtgt gggtgttatt gttgccgtat tcagtgtgaa tcaatataaa
     6901 aacaggcatc ttgaaaccat taaagagaag ataaattcta tttatacgga gctcgatgac
     6961 cgacttgctg aagagcctga tctggcagcc gactggaggg atgcagcaaa tccttcgctg
     7021 aatgaatttc ttataaaatt atcgaatgtg ttcaatacag atataaatct ttatgacaga
     7081 aatggttatc tgatggctac atcgcgacca gaagtgtttt accgcaacct gacaagccgg
     7141 cggctcaata tggatgcatt tataaatctt gaaaatctta caaaaagtga gtatatccag
     7201 cgtgagaaaa tagtttcact tgaatattta tcggcatatg taccattcta taatgagcag
     7261 ggctatctgc ttgcatatct taaccttcca tatttcagga tggagagtgt actggcacgt
     7321 cagatatcaa atgtgattgt ggcggtggtg aatttcacac ttcttattat aataatcaca
     7381 atggttattg ctgttttgat aagtcggcga cttacagctc cgctgaggat gttgagtgaa
     7441 ggacttgcct cggtgcaact tgggaaaaag agcgaacatc ttgtatatag gggtcatgat
     7501 gagatcgggg agatggtcag ccagtataac cggatggttg atgagctgca ggaaagtgcc
     7561 cgaaaacttg ccgactcgga aagggaatat gcatggaggg aaatggcaaa acaggtagca
     7621 catgaaatca agaaccctct cacacctatg aaactgaatg ttcagcagct ttacaaatca
     7681 tggaaagatg gtacgcccgg gtttgaaaag aagctggaaa aatttacaaa gaatcagatt
     7741 gaatacattg agactctctc atctatagct tctgcgttct catcattcgc aaaaatgcct
     7801 tcagcaaacc cgatggatct ggacctggtt gaacagttga gaaccacact cgaacttttt
     7861 aaagatactg aaaacatatc gttcaggatt gactggccag ttgatacaaa aatttttatt
     7921 tatgccgata aagaacacat aatcagtatt ttttccaatc tgataaagaa tgccattcag
     7981 tcgattcctc agggcaggag cggattaatt aaaatcgggt tggaacttaa cggagacagg
     8041 gctattgtgt cggtggctga caatggtaca ggaataccag aagacttaaa accaaaactt
     8101 ttcacgccca atttcactac aaagtcttcg ggtatggggt tgggtctttc aattgtaaaa
     8161 cggtatgtcg aaaccgcagg tggaagagta tggtttgaat cggaaaaaga cagtggttcg
     8221 gtcttctttg tggaacttcc tgtaaaattc accgtagaaa agtctggaca ggctaagtaa
     8281 aaatactctt gataagttgt aaaattattt agttatatta gcagattcag acagagctcc
     8341 gatgatattt aaaatgtcag gtaacatatt ttcattcctg gcgggatttc taatgttgat
     8401 attaagtatc agcgcacctg cacaaaaaaa tctttccggt gttttaggca tgcctgccgc
     8461 ccatgttgtt tcaatcggag ttgaccgtgt aacagttgat gatgtgaccg ggttcaatat
     8521 cgccggcgga gatactgttc ttgttataca gatgcagggt gtaatgatac tggatgaccc
     8581 gggctcttat ggccagttac aggataccat aggcagacct ggtttgtggg agtttcttat
     8641 tacacagagt gttaataccg gaacaaagga aattattttc aggaatgagt taaaaaacaa
     8701 ttatggaaca gagggtaatg ttcaggtcgt aagggtaccg ttctataaca gtgtagatgt
     8761 tactggaact cttacagtta acggatggga ccctgtgaaa aaaaccggag gtgttctagc
     8821 tatgattgtc gggcgcacct taaaactttc ggccgatatt gatgttaccg gaaaaggttt
     8881 tacaggtggg aaggacacaa taggtgatgg catatgcagg gctacagatg agactacgta
     8941 cggcaaacca ttttatccga gaacttttac caatgctggt tataaggggg aagggattgc
     9001 caattataca aaggatggaa cgttacttgt acctgattat ttaaaaggtt atggtcccaa
     9061 ttataccggg ggaggcggag gtaacggaag atttgctggg ggaggaggcg cttcgcttat
     9121 tggcgccggt ggtacaggag gttttgagga tttcttttgt tcaccaaatc ctggagaagg
     9181 ggggacaggg ggaataaaag cagatcaccc ttcaatctca ggcggtctct ttctgggagg
     9241 tggtggtgga agttcaacaa gagcttcaac cggaggaact gccggaccag ggggtaatgg
     9301 aggcggtata gttattattg ttgcagattc tattatagga aatggtggta acatacgtgc
     9361 ttctggagct cctggaggca atgcatcggg cagcggagga gcaggcggcg gaggagcagg
     9421 cggttcaata gccctgtcgg ttaaaaatta tggtacatcg gcaatgtcgc taaatgttag
     9481 cggagggaaa ggaggcgaca ggcttcctgg taatggagga gaaggcggcg gaggcggagg
     9541 tggcctcata tgggttagca cggctatgcc tgcaaatatc tcgacagacc tgagtggagg
     9601 tgaagcagga atgagttcaa cgccattggc cagtcccggg caaccgggcg aaaagaaaac
     9661 tgacttcaag gctgcactta atggatttct gtttaactca ataagatcat ccattacagg
     9721 taatcaggtt gactcagtat gctcaaacat gctgcctcca ttgattacag gtacaacacc
     9781 tgtaggaggc gtggaaccct atacctatac atgggagaaa agctacgacc agagtacctg
     9841 gatacaggtt gccagcggaa cagatgcttc atacattaat tataccccta ctgtagtcga
     9901 aacaagtact gtatggttca ggagaataat aacagattct tcttctccac tgccattgat
     9961 tgatgtgagc aaacctgttc aaattatcgt ccagccctat atcaagaata atattatcgg
    10021 cacctccgat actatatgct ttgcacagga tcctccaacc tttacctcta aggcaacatt
    10081 gcaggacggc aacggtatat attacttcag atgggaagcc agtaccgata gcaataaatt
    10141 tatattgcct gtaaatacat ataatgaaga gaattacact ccgccacctg ctttaaagca
    10201 aacatcatgg tacagacgca cagtaatctc aggaagatgt attgattctt cggctgttgt
    10261 gaagattact gttcttgaca caataaaaaa caatacaata cttactcctg ctgaagagat
    10321 ttgttatggg atgttgttta ctgatataag tgcaactacc cctcctgtac tggccggagg
    10381 tgacggtatt taccgctata aatgggaaag cagtcctgat ggcacatcgt ggtcgaatgc
    10441 tgaaggaaca gttaacaatt cggattacaa ccctgttgaa tcgtcatcgt cgttcccggg
    10501 caccctttat ttcaggagag ttgtttattc aggaagtaat gatgtttgtg taaaccacag
    10561 tgcacctgtt cagctgactg cctggcctgt aattggcaat aatactattt cagccgatca
    10621 gactatctgt tcgggaagca tacctgcagc actaaccgga acactccctg taaacggaaa
    10681 cggaacatat tcatacctgt ggcaaaacaa aacaaaatcg tcgtcatggg tcacagcagc
    10741 aggtcccaat ccttttaacc agcaaaacta tatgcctcct gcactcactg attcaacatg
    10801 gtatagaaga gtggttactt cttcagcctg cagcgataca agcaatgtga ttgtggttaa
    10861 tgttcataaa ccaatctcaa acaataatat ccagcttttg tcggggctga cagatactac
    10921 catatgcagc ggaggcaagc ttaacctgtt tattggaagt acccctgccg gcggaacaga
    10981 tttacccggg gattatttgt accagtggga gacttctcct acggctgttg gtggatatgt
    11041 tcccattaca ggtgcaactt caaaagattt tcaaagtgat gtcatcatta atccaacatc
    11101 tcttccggtg gatcactatt tcaggaggtt tgtttcctca gggacctgca gcgcaccgag
    11161 cagccagata ataaaagtaa cagttctgcc atcaataacc aataatgtta ttacaccaga
    11221 caaaaccgct gtctgttata atacttcacc cgttatctcc gggactcctc ttaccggagg
    11281 agccggcgga actcccacat ggctatggat agagagtccc gatggaatta cttcatggag
    11341 tccggcaagt ggaacaaata accagcaaaa ctatacgccg cctcctttaa caagccctgt
    11401 attttacaga agggtaatcc tttccggtcc tgcaaactgc tgtattgata catcaaatgt
    11461 agcgggcatt agcatcaacc cgctgccaga aggatatata acttccataa ctgatacaac
    11521 agtttgcagc gggatacagg tgccggttaa gattcatctg acaggcgctt caaaatggaa
    11581 tctgacatat actgaaaatg gaactccagt agtactgaat aaaataatga atcaggatac
    11641 ggtaatcatt attaataaaa cgccttccgg tgatgtggaa atatataatt atgctttgca
    11701 gggactggta gatgcaaatg gatgtaatgc aacagtactt acaggttcga gaaagattac
    11761 agtttacaga acaccagttg ccaatgcagg tgctgatgaa gcagtatgtg gaccggtata
    11821 taccctgaag gcaacaccaa gtgtcggaac aggttcatgg ctgttacctt ccgacgttac
    11881 caacctcact gcccctggac cttccatgac ggtagctatt gattctttga atgcaccggc
    11941 gatgctaaaa aggaaattct actgggagga aattaactgg acatgcactg ataaagattc
    12001 ggtggagata acattctaca aacgcaccgg acaggcgaat gcaggacctg acaaagatct
    12061 ttattcgttt gaaaaggtcg atactctgca tgctgcaaaa cctcttgtag gaacaggtac
    12121 atggagtgtc ctttcgggaa gtgcacaaat agtaaaagat tctatagtta taaacctttc
    12181 accgggagtg aacaggttcg aatggaaagt agtcaatggt ttgtgcgaat cgaaagacga
    12241 gatggttatt aatgtgcatg agctggtggt tcctgaagga ttttcgccca atggcgatgg
    12301 aattaatgat gaatttgtta tcctggggct tgatcttaat tacaatgaat gttctcttag
    12361 agttctcaac agtgccggaa gcgaagtttt ctttaccacg aatgcaaatg gccgtacatg
    12421 ggagcactgg aatggagaga gtggagggaa tccgctacct gaaggaacat attattacat
    12481 gctgactata aaatcaaaaa gaactgattc gtcattcaaa aaaagcggat ttgttgtgtt
    12541 gaaaagatac aattactgat tataataaat ttagtatttt cacggctgtt ttacagatga
    12601 aaggaaggct gtttttactg atgcttatta tgtcgtgtct gcacctcagg ggacaggacg
    12661 gaacttcaga tatgacgaag ataagctttt tctatcctgt ttacagccag tatctgcata
    12721 acggtcttgt gattaatcct gcatatgcag gcacacgcga agctctgagc ttttttgtat
    12781 caggacggat gcaatggatg ggtattgaag gtgcacctgt attccagact gcttcattgc
    12841 ataccctgct gaagaacaat aaagtcggac tcgggttatc gggacagttt tttaggtatg
    12901 gttatacacg tgcaacaagt gtttatacgg actatgcata tcatattgtt attggaaaga
    12961 gccggctatc aatgggtctg agagcagggt ttgatatgtc gaatactgat tataccggaa
    13021 taatactcat tgacccgaat gatcctgttt ttataacgaa cgacaaacct tatatgcttc
    13081 caaatgttgg agctggtata tatttttcaa ataaaagatt ttttatcggt gcttcggtac
    13141 catctttcct gacctatctg aaaagtagtt caggcgagat aagctataat acattcaggg
    13201 aattcgacgc aaacttaact gcaggggctc tgatttcgtt ttcaaattca tttaaattta
    13261 aaccttctgt tctggtaggt tatcctttcc gggagaagac aaataatgat gaaataagaa
    13321 tagatctgaa cggaaatttt atattttttg actttttatg gctcggggct tcatggcgga
    13381 catatgaaga tgttattgtg ggtattatac aactacaggt aacccctcag cttatgttcg
    13441 gttattcgta cgattatcct tcaggtcaga tgagcacata ttcaaaaggc tcacatgaag
    13501 ttatacttag atatgagttc ggttacaagg tgtcagccac aaatccaaga tatttttaag
    13561 gtatggaaag atggtggatc catattgtaa ttttttcttt atgtttaatt tgtggcagag
    13621 actatctttt ttcacagcca cttgtatata aaattgaaaa aatgcctttc tcttcatccc
    13681 agttcaatga tattgctccg gttattataa aagatggtat aattttttgt tccgacagac
    13741 gtgcgagcag cgctaaaaat gttactactt ttaatgaaga gagactttat aatctctatt
    13801 tttcagcaag gcgtgacact ctaaaatgga gagaacctta cgagataaaa atttcaggtg
    13861 accctgtgct tttcaccggg cctgtttcag tttcggccga tggttcgacg atttacttca
    13921 ccagcagcgt tctctcgggt aaagcagctc gtaaacgaaa tatcaagaac actctcggta
    13981 tttatgtcgg agagctgtcg gg
//
LOCUS       DAOSNB010000029        13988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4387_length_13988_cov_3.975095, whole genome shotgun sequence.
ACCESSION   DAOSNB010000029 DAOSNB010000000
VERSION     DAOSNB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_13988_cov_3.975095"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..1157)
                     /locus_tag="PLG42_02400"
     CDS             complement(357..1157)
                     /locus_tag="PLG42_02400"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOU30093.1"
                     /translation="MKDLLDLTGKVALVTGGTHGIGLAIGLLYAKAGARVCVNDLSEE
                     KLENCRNEYQKEGLDVYTLKFNVTDEEDVDRGITEIEKNVGEIDILVNNAGIIKRIPI
                     LDMKVSEFREVLDVDLVGPFIVSKRVAPGMIRKRSGKIINMCSMMSVYGRNTVSAYAA
                     AKGGLKLLTQNMTCEWAKYNIQVNGIGPGYIATAQTAPIREGNHPFNDLVMTRTPAGR
                     WGTPEDVAYAALFLASKASNFINGHILYVDGGILANFGYVKGENEVKP"
     gene            complement(1396..2232)
                     /gene="kduI"
                     /locus_tag="PLG42_02405"
     CDS             complement(1396..2232)
                     /gene="kduI"
                     /locus_tag="PLG42_02405"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOU30094.1"
                     /translation="MKTELRYAVHPSDFRHYDTARIRKEFLIEKLFEKNELIFIYSMY
                     DRYIVGGAMPVDRPVVLETFDELKSENFLDRREMGIINVGGDAEVRTNSGVFKLAFKE
                     ALYLGRGTKGVEFISLNSLKPAKLYINSAPAHHTYPSRKVTLADAEVVELGSPEQSNH
                     RTINKLLVNSVIETCQLQMGMTELKPGSVWNTMPVHTHARRMEAYFYFEVPEKQAVCH
                     FMGDPDETRHIWMVNEQAVLSPSWSIHSAAGTSNYTFIWGMAGENLDYGDMDVRYPDS
                     LK"
     gene            2387..3781
                     /gene="uxaC"
                     /locus_tag="PLG42_02410"
     CDS             2387..3781
                     /gene="uxaC"
                     /locus_tag="PLG42_02410"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOU30095.1"
                     /translation="MSFIHPEFLLTNKTASRLYHQYSEDKPIIDFHCHLSPQMIATDR
                     QFENLAQIWLEGDHYKWRAMRTNGVPEKYCTGDASPVEKFKKWAETVPYTVGNPLYHW
                     THLELARYFGIFDLLSPSTADRIYNQASEMLKTKEFSTRSLIRKMNVEVICTTDDPVD
                     TLEYHRQMKGDFEVKVLPTFRPDAVLKIEDTEKFVQYIRKLEQSADTGIRDFSSLVAA
                     LDKRHQFFHDNGCRQSDHGLDRFFYAEFSESEIDRIVKRLLSGKPLNDGDPEKYKCAI
                     MLELCRMNHKRGWTQQFHVGAMRNNNLRMFRLMGPDTGWDSIASPQDALKMSRFLSKL
                     DESDQLARTILYNLNPADNEVMITMAGNFNDGTVPVKVQYGAAWWFLDQKTGMEKHLR
                     DLSALALLSRFIGMVTDSRSFLSYPRHEYFRRLACNYIGEEVEKGLIPNDEELLKLIV
                     EGISYRNAKEHFGF"
     gene            3842..4687
                     /locus_tag="PLG42_02415"
     CDS             3842..4687
                     /locus_tag="PLG42_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU30096.1"
                     /translation="MNNLLNVKDKIAVITGGAGVICSNMARALAAQGVKTVILDLNGE
                     AARSIAEEIENEFKVSSLGLSANVLDRDSLVKAKKVINDKLGTIDILINGAGGNSPAA
                     TTKAERIEGAENENLEDTFFGLKIEGFDRVFDLNFKGTLLPCLVFATDMVKKKSGVIV
                     NISSMNSYRPLTKIPAYSAAKAAINNFTQWLAVHFAKTGVRVNAIAPGFLLTNQNRFL
                     LIDEKTNELTPRGKKIIKGTPMERFCKPEELTGTLLFLVSEMSSFITGVVIPVDGGFS
                     AYSGV"
     gene            4797..6083
                     /locus_tag="PLG42_02420"
     CDS             4797..6083
                     /locus_tag="PLG42_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOU30097.1"
                     /translation="MENGMIYFGTGSEKAILSSDDLKNGLFTALEKIGRRKKVLVIPP
                     DFTRYHSRAGELTGYIYKYYGNNVSDILPALGTHSPMTESEIETMFPGVPKNLFRVHD
                     WRNDVVTAGVVPGEYISEITGGKLTYSWPAQVNKLLKNGGHDLILSVGQVVPHEVTGM
                     ANYTKNILVGTGGSEGINKSHFLGAVYGIEKLLGKPDNPVRQLLNYAAAKYLSDLPIV
                     YVLTVIGRDETGKLVARGLYIGDNPDVFKLASDLSLKVNFTVLDKPLQKVVVYLDAGE
                     YKSTWLGNKSIYRTRMAMADNGELIVLAPGLKEFGEDKEIDRLIRKYGYRGTPATLKA
                     LQENQELQNNLSAAAHLIHGSSEGRFNITYCPGHLSREEIESVGFRYGDLNEMIKKYN
                     PEKLKEGINILPGGEEIYYISNPATGLWSTKERLYE"
     gene            6149..7183
                     /locus_tag="PLG42_02425"
     CDS             6149..7183
                     /locus_tag="PLG42_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30098.1"
                     /translation="MDGGIYRLVFRIENRSMINWGIIGCGNVTEVKSGPAFNKVPGSR
                     LVAVMRRNRALAEDYARRHNVPRYYDKAEDLINDSEVNAVYIATPPGSHAQYAIMAIE
                     AGKPVYVEKPMALNYRECQMINEAAEKYNIPVFVAYYRRTLPGFLKVKELIENGAIGN
                     VRFFSIQLFKAPSEDEKSGKLPWRVKPEISGGGHFFDLASHQIDYLDFLFGPVFQVKS
                     VVLNQGGLYKAEDYVSVQFVFGNGVTGNGTWCFSLDHSSNRDLMEFFGDRGSIKLSCF
                     TFEPIVVTSSYGTREYINERPENVQYYLIEQVVNELLGKGKAVSTGISGARASKVMDE
                     IVEEYYASNS"
     gene            7293..8747
                     /gene="gnd"
                     /locus_tag="PLG42_02430"
     CDS             7293..8747
                     /gene="gnd"
                     /locus_tag="PLG42_02430"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOU30099.1"
                     /translation="MNNLSDIGLIGLAVMGENLVLNMESKGFRVTVYNRTVQKVDDFI
                     NGRAKGKNIVGAHSIPELVASLKKPRKVMIMVKAGSAVDDMIEQLLPHLEKGDIIIDG
                     GNTHFPDTNRRTAYVESKGLLYIGTGVSGGEEGALLGPSIMPGGSKAAWPEVKPIFQA
                     IAAKVDDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQLICESYHLMRSLLGMTPDEMH
                     LVFKEWNEGELNSYLIEITRDILAYKDTDGQPLIDKILDTAGQKGTGKWTGITALELG
                     IPLTLIVEAVFSRCLSAMKDERVIASRILKGPEASFKGSREEFLNNLREALYASKIVS
                     YAQGYSLMRAAAQEYKWDLNYGGIALMWRGGCIIRSVFLGKIKEAFDKNPGLTNLLLD
                     PFFREKVEKAQNGWRNVVATAALNGIPVPALSSALSYYDGYRSEKLPANLLQAQRDYF
                     GAHTYERVDRPRGEFFHTNWTGRGGTTSSTTYNV"
     gene            8820..10118
                     /locus_tag="PLG42_02435"
     CDS             8820..10118
                     /locus_tag="PLG42_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU30100.1"
                     /translation="MTEAVKKIGNYRWTICALIFFATTINYLDRQVIGILKPLLESDL
                     NIGEKAYSHIVMAFQFFYAFGMLLTGRLIDKWGTKIGYGVAVVVWSLAAMGHALAKGA
                     LGFGFWRSLLGLGESANFPAANKGVAEWFPKKERALAFSILNSGTNVGAIVAPLAVPA
                     IVALWGWQAAFIITGAIGFIWLFFWFWIYEVPSKKKNLSKEEYDYIHSDVEEQNESQE
                     SVPWLKLLKYRQTWLFFIGKAMTDPIWWFYLFWIPGWLADVRGTGLNVKSFGLPLAFI
                     YTSTTVGSIFGGWLSSYMIKKGMSPFRARSIAMLIFAILVTPIAFASSPSISTWGAVC
                     LIALAASSHQAWSANIFTTVSDAFPKKAVSSVTGIGGMAGALGGLGVSYFAGFILDLY
                     KKAGHVETGYMVMFAIAASAYLIAWLIMNVFAPKNKKVEL"
     gene            10141..11205
                     /locus_tag="PLG42_02440"
     CDS             10141..11205
                     /locus_tag="PLG42_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU30101.1"
                     /translation="MFSRKARLKDIAALAGVSIGTVDRVLHNRGQVAEKTQERILKIA
                     RELNYTPNIMARALKTKKVFTLMSLLPAPSDENAFWQKHPEGMEKAMAELNQFPVNLE
                     QVTYNFYSETDFQKKAKEAIKKNPDGIILAPLFRSQSIQFCSELKNKKIPFVFVDNYL
                     TETNFLAYIGEDIFRSGRVAGQLADLLTAADEDILIVNIAKNIRNMHHLNKRVKGFRS
                     YFSGSDRMKSKIFILNIPDTKRKTVKEMTDKILSVHPGIKTIFVTGSKSFIIAEYVHS
                     SGHKQMNIIGYDLIDKNVEYLRKGIIRFIIGQRPEEQTYRAVKKLFDYLSFNKLPEKM
                     EYLPVDIVTSENVDFFINNI"
     gene            11225..12478
                     /locus_tag="PLG42_02445"
     CDS             11225..12478
                     /locus_tag="PLG42_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOU30102.1"
                     /translation="MKKLGKYSMGIGDRFGRQAGAQLKAIIDAGKKGVEITPVWNKSN
                     REHIIIGTEPADVRKEADEAVKAAKFKKPYFVDADHINFDTVGRFIEASDFFTIDVAA
                     YIGQRACEEDIKAFVKKAAKYKGKLRIQGIPKPFNITSAYLKKVAEKYLFATKMAGVI
                     YRKIEKLKGAGKFVTEVSMDEVPDPQTPAELFFILMMLSIENIPLQTIAPKFQGRFNK
                     GVDYVGNPELFALEFETDLLVIRHAVKLFKLPENLKISVHSGSDKFALYPHIGSIIRK
                     YDAGIHLKTAGTTWLEEVTGLAMAGGEALTFVKEMYKSAYEKIDELCAPYADVIDIKK
                     ERLPSPAEVMKWDSEKLSSAIIHDAKNPNYNPDMRQLIHVGYKLAALRMKEYLELLDK
                     NKDVVSRCVYENIYSRHICRLFGIG"
     gene            complement(12560..>13988)
                     /locus_tag="PLG42_02450"
     CDS             complement(12560..>13988)
                     /locus_tag="PLG42_02450"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOU30103.1"
                     /translation="ENVQTLFTGVDSLWPGKNTVKDIFGKAEKISRVLNFKGEQALST
                     GDNYFWVVCNLSHSTSLHNRIDFGFSSVRITGCRKMEVIEINTGIRQRVGVAVRKHLD
                     DNVHSYRIPGLVTTKAGTLLAIYDVRRLSSRDLQGDIDIGLSRSTDGGNTWEPMRIVL
                     DMDEWGGLPQKFNGVSDACILVDENSGKIFVAGLWMHGVLDTAGRWIEGLTEESNAWE
                     HQWRRKGSQPGFDVRQTSQFLITTSTDDGKTWSEPENLTRMCKRREWWLLAPAPGRGI
                     TMDDGTLVFPAQGRDENGVPFSNIIYSLDGGLTWKTSNPAFSGTNECAVVQLDDGTLM
                     LNMRYGQRQKGETGRAVAVTSDLGKTWREHTSSRNTLREPVCMASLLKHECSLNGEKK
                     HVLVFSNPDVESPPRRNITIKVSFDDGLTWPEEYYFLLDEGTGRGYSCLTSVDERTIG
                     ILYESSQADLVFESIPLEELLRINK"
BASE COUNT         4189 a         2928 c         3077 g         3794 t
ORIGIN      
        1 taattttaaa caagttataa aatttaccgg aaaatagaaa gggatagtta attatttaaa
       61 atgtgtggtc taaaaaagtt aacagtttat tattacccag gtaacccttt ttatttgctt
      121 ttatgataac tggacccttt taaatttcgc cccctttttt gtttcctttt gtggtataca
      181 gacctctttt gtgtttcccc gagggggaat tttatagtgg aatacaccct ctaatccccc
      241 taagaggggg atttaaatat cacactcacc aaagtagttt agcagtaaaa aaacaaactt
      301 gtaaacttat accaggatca aatgaaaaac ttagaatttt ttcagaatga cacagtttat
      361 ggttttacct cattttcccc cttcacatat ccgaagttcg ccagtatgcc tccgtcgaca
      421 tacaggatat gtccgttaat gaaattgctt gcctttgagg caaggaagag tgctgcatag
      481 gccacatctt ccggtgtacc ccagcgacct gccggagtac gggtcatgac gaggtcgttg
      541 aacggatggt ttccttccct gatgggagct gtctgcgctg tcgcaatata gccaggtcca
      601 ataccattca cctgaatatt atattttgcc cactcgcatg tcatattctg cgtaagaagt
      661 ttcagacctc cctttgcagc tgcgtatgcc gagactgtat tgcggccgta aacactcatc
      721 atcgaacaca tattaattat tttacccgac cgcttcctga tcatgccagg ggcaaccctt
      781 ttcgaaacaa tgaatggccc cacgagatca acatccagca cttccctgaa ctccgaaacc
      841 ttcatatcaa gaatcggaat tcgctttatg attccggcat tgtttacgag aatatcaatt
      901 tcaccgacat ttttttcaat ttcggtaata cctctgtcaa catcttcctc atcagtaaca
      961 ttgaacttca atgtgtaaac atccagacct tctttttgat attcattcct gcaattttca
     1021 agcttctcct cactgaggtc attcacacat acccttgcgc cagcctttgc gtaaagaagg
     1081 cctattgcca gtccaatacc atgtgtgccg cccgttacga gggctacttt tcctgtaaga
     1141 tcaagtaagt ctttcatgat tagataattt atttatgttt caatataata ttattctttt
     1201 gattattttc attccttact gtcagaaagc gagcatcata atctatcagc ttaaatgcct
     1261 tacttacatc catgacaggg gttgtctaaa aagcctcttt ttttcacccc tgaatccccg
     1321 ctttactgct gcaatgtcgt acaggcccgg agagggtatt taccgatata taatgtagtt
     1381 tatatgattt taaaactact tcaatgaatc tggatatctt acgtccatat cgccgtaatc
     1441 gagattctcg cctgccattc cccagataaa tgtataattt gacgtacctg cagcgctgtg
     1501 aatgctccac gaaggagaca gtactgcctg ttcgtttacc atccatatat gtcgtgtttc
     1561 gtcagggtcg cccatgaagt ggcatactgc ctgtttctcg gggacttcaa aatagaaata
     1621 tgcttccatt ctgcgtgcat gagtatgaac aggcatggta ttccatacac ttcccggttt
     1681 aagctctgtc attcccatct gcagctggca tgtctctatt acactgttaa ccagtagttt
     1741 attaatggtt ctgtggttgg attgttcagg cgagccaagc tcaactactt cagcatcagc
     1801 cagggtaacc tttcgtgaag ggtaggtatg atgcgcaggt gctgaattga tatatagttt
     1861 agctggtttt aaactgttaa gtgagataaa ttcaacaccc tttgttcctc tccccagata
     1921 aagagcctcc ttaaatgcaa gtttgaagac gccgctgtta gttcttactt cagcatcgcc
     1981 acctacatta ataatgccca tctcgcggcg gtcgaggaaa ttttccgact tcagctcatc
     2041 aaacgtttca agcacaacag gacgatcgac cggcattgca ccccctacga tatacctgtc
     2101 gtacattgaa taaataaata taagctcatt cttctcaaac aacttctcaa taagaaattc
     2161 tttccttatc ctcgccgtat cataatgtct gaaatcggat gggtgtaccg cataccttaa
     2221 ttctgttttc atgttaaact gattttaaaa taatgacata tcattaattc aggtgtaaaa
     2281 ataaatactt atgccgattt tttttgcatt atttggttta atttgtattt tcgtgttcgt
     2341 taacgcaaaa ttattctatt tgtcaacatc ttaaaaattc aactttatgt catttattca
     2401 tcctgagttt cttctgacaa ataaaacagc cagcaggctt tatcatcagt actctgaaga
     2461 caaaccaata atagactttc attgccatct atcgcctcag atgatagcaa ccgacaggca
     2521 atttgaaaat cttgcccaga tatggcttga aggagatcat tacaaatggc gggcaatgag
     2581 aactaacggt gtgcctgaaa agtactgtac aggtgatgca tcacccgttg aaaaattcaa
     2641 gaaatgggct gaaacagttc cttacactgt aggtaatcct ctttatcact ggactcatct
     2701 ggaacttgcc agatattttg gaatattcga cctgctctcc ccttcaactg ccgatagaat
     2761 ttacaatcag gcaagtgaga tgctgaaaac aaaagaattc agtacaaggt cgctgattag
     2821 aaagatgaat gttgaggtca tctgcactac tgatgaccct gttgatactc ttgagtatca
     2881 caggcagatg aaaggggatt ttgaagttaa agttttaccg accttcaggc ctgatgcagt
     2941 tctgaagatt gaagataccg aaaagtttgt gcaatatatc cgtaaacttg aacagagcgc
     3001 ggataccggg atacgtgact tcagttctct ggtggctgct ctcgataaaa ggcaccagtt
     3061 ctttcatgat aacggctgca gacagtcgga tcacggactc gacaggtttt tctacgcaga
     3121 attcagcgaa tcagaaatag acaggatagt taaaagactt ctcagcggta aacctcttaa
     3181 cgacggtgat cctgaaaagt acaaatgtgc cataatgctg gagctgtgca ggatgaacca
     3241 taaacggggc tggactcagc agtttcatgt tggagcaatg aggaacaaca atctgaggat
     3301 gttccggctg atgggacccg acaccggatg ggactctata gcctcaccgc aggatgctct
     3361 taaaatgtca cggttcctca gtaaactcga tgagagtgac cagctcgcaa ggacaattct
     3421 ttacaatctg aatccagccg ataatgaggt tatgataacc atggccggca atttcaacga
     3481 cggcacagtg ccggttaaag ttcagtatgg tgctgcatgg tggttcctcg accagaaaac
     3541 tggcatggaa aagcacctca gagacctttc agctctggca ttattgagca gattcatagg
     3601 aatggtaacc gactcgagaa gttttctgtc gtacccacgc catgaatact tcagaagact
     3661 ggcatgcaat tacataggtg aagaagttga gaaaggactc atccccaacg atgaagaact
     3721 tctgaaactc atagtcgagg gtataagcta cagaaatgct aaagaacatt tcggattctg
     3781 atttatttat acaaaaaatt ctttccggaa aaattatcaa taaaactaaa attaattata
     3841 aatgaacaac ttactcaatg taaaagacaa aattgcagta attaccggcg gtgcaggggt
     3901 gatttgttca aacatggcaa gggctcttgc agcacaggga gtaaagacag ttattcttga
     3961 cctcaacggc gaagccgcca ggtctatcgc cgaagaaatc gaaaacgaat tcaaggtttc
     4021 ttccctgggg ttatcggcta acgtactcga cagggattca cttgttaagg caaaaaaggt
     4081 tatcaacgac aaactaggta cgattgatat ccttattaat ggtgccggtg gaaactcacc
     4141 tgcagccacc acgaaagccg agagaataga aggagctgag aacgagaatc ttgaagacac
     4201 ctttttcggt ttgaaaatcg aagggttcga cagggtgttc gatctgaatt tcaaaggtac
     4261 tctcctgccc tgtctggtct tcgctaccga tatggttaag aaaaaatcgg gtgtcattgt
     4321 taacatttct tcaatgaact catacaggcc gcttacgaaa atccctgcat actctgctgc
     4381 caaagctgca ataaacaatt tcacccagtg gctggcagtg cactttgcaa agacaggcgt
     4441 aagggttaat gcaatagctc ctggatttct gcttacaaac cagaacaggt tccttctcat
     4501 cgacgaaaaa actaacgagc tcacaccaag agggaaaaag attatcaaag gcacgccgat
     4561 ggaacgcttc tgcaaaccag aagagctgac aggaacatta ctattccttg tgtcggaaat
     4621 gtcgtcgttt atcacaggag tggtaattcc cgttgatgga ggattcagcg cttacagcgg
     4681 tgtataatag ttaacaacta gccattggaa gcaaaaattt ggcaccagac agctggcatt
     4741 caaagttcaa ggttaaaagt tctgtgtctg gcaacattac tttataagaa gaataaatgg
     4801 aaaacggaat gatttatttc ggaacaggtt cggagaaggc aatattgagt tctgatgact
     4861 taaagaatgg cctttttacg gcactcgaga aaataggccg gagaaagaaa gtacttgtta
     4921 tacctccaga ttttactcgt tatcattcaa gggcgggcga gttgacagga tacatatata
     4981 aatattatgg caataatgtt tcagacattc tgcctgcact tggaactcat agtccaatga
     5041 ctgagagtga aattgaaacg atgtttccgg gggtaccaaa aaaccttttc agagttcacg
     5101 actggagaaa tgacgttgtt actgccggtg ttgttccagg ggagtacata tcggaaatta
     5161 caggaggaaa gctcacctat tcatggccgg cacaggtaaa caagctactg aaaaacggcg
     5221 ggcacgacct tatactttca gttggacagg tagtgccgca cgaagtaaca ggaatggcta
     5281 actatacaaa gaacatactt gtaggaacgg gaggttcgga aggaatcaat aaaagccatt
     5341 ttcttggtgc tgtatacgga atagaaaagt tgcttggcaa accagataat ccagttcgac
     5401 aactgcttaa ttatgcggct gcaaagtatc tcagcgacct gccaattgtt tatgtgctta
     5461 ccgtaatcgg tcgcgatgaa acaggtaaac ttgttgccag aggcctttat ataggcgata
     5521 atcctgacgt attcaagctt gcatcagatc tttcactgaa agtaaacttt accgttcttg
     5581 acaaacctct gcagaaagtt gttgtttatc tcgatgctgg tgaatataaa agcacatggc
     5641 tgggcaacaa aagcatttac cgtacaagaa tggcaatggc agataatggg gagcttattg
     5701 ttcttgcacc ggggctgaaa gagtttggag aagataaaga gatagacaga ctgataagaa
     5761 aatacggtta cagaggtaca cctgccacac tcaaggcact gcaggagaat caggagcttc
     5821 agaataatct cagtgctgca gcgcatctga ttcacggcag ttcggaaggc agattcaata
     5881 ttacttattg tccgggtcat ctgagccggg aagaaataga gtcggtcggt ttcagatacg
     5941 gagatttgaa tgagatgatt aaaaaatata accctgagaa acttaaagaa ggtataaata
     6001 tactacccgg tggtgaagaa atttactata tttccaaccc ggcaactgga ctatggtcga
     6061 ctaaagaaag attgtatgaa tagaatgttg ccagtcttct gcaccaggga cctggtgtct
     6121 taaaggtgca gggtattccc gggataaggt ggatggggga atataccggt tagttttccg
     6181 gattgaaaac agaagtatga taaactgggg tattataggt tgtggtaatg ttacagaggt
     6241 aaaaagtggt ccggcgttta ataaggtgcc gggctcaagg cttgttgccg tgatgcgaag
     6301 aaaccgtgct ctggctgagg attatgccag aagacataac gtgcctcgtt attacgacaa
     6361 ggcggaagac cttatcaacg acagtgaagt aaatgcagta tatatagcaa ccccacctgg
     6421 aagccacgct caatatgcaa taatggctat tgaagcggga aaacctgtat atgtcgaaaa
     6481 accgatggct ttgaactaca gggaatgcca gatgataaat gaagctgccg aaaaatacaa
     6541 tattcctgtg tttgttgcct actaccgccg cactcttccg ggttttctga aagtaaaaga
     6601 acttatcgaa aacggagcaa tcggcaacgt caggtttttc agtatccagc tgttcaaagc
     6661 tccctcggag gacgagaaat caggaaaact gccatggcgt gtaaaacctg aaatatcggg
     6721 aggaggacat ttttttgacc ttgcatcgca tcagattgac tatctcgact ttctgttcgg
     6781 tccggtattt caggttaaat ctgtagttct gaaccagggt ggtctatata aggctgaaga
     6841 ttatgtgtcg gtacaatttg tgtttggtaa cggagtaaca ggaaacggca catggtgttt
     6901 cagtctcgac catagttcaa atcgtgatct aatggagttt ttcggagaca ggggtagtat
     6961 aaagctatcg tgctttacct ttgaacctat tgttgttaca agcagttatg gtacaaggga
     7021 atatataaat gaacgtccgg aaaatgttca gtactacctc attgaacagg ttgtaaacga
     7081 actacttgga aaaggtaaag ctgtgagtac cggcatcagc ggagcaagag caagtaaagt
     7141 aatggatgag attgtggaag aatattatgc atcaaatagt taaaggaata gagcttaaga
     7201 aacgaagcga agtcccgggt agcaggagag ctcagagtta aaaatttaaa attgagaagc
     7261 aatatattta ataaaccaat aaataattaa ctatgaacaa cttatcagat ataggattaa
     7321 ttggattggc ggttatggga gaaaaccttg tacttaacat ggaaagcaag ggtttcagag
     7381 ttactgtgta taaccgcact gtacaaaaag tagatgattt tattaacgga agggctaagg
     7441 gtaaaaatat tgtcggggct cattcgatac cggaacttgt tgcctcgctc aagaagccca
     7501 gaaaagtcat gattatggtg aaggcaggga gtgctgttga cgacatgata gaacagttgc
     7561 tgcctcatct ggagaagggt gatataataa ttgatggagg caacactcat tttcctgaca
     7621 cgaacaggcg tactgcctat gtggaaagta aaggcctgct gtatattgga acaggcgttt
     7681 cgggaggtga ggaaggtgcg ttgctgggcc cttcaataat gcccggtggt tcaaaggctg
     7741 catggccaga agtaaagcct attttccagg ccatcgctgc caaggtagac gatggcacac
     7801 cgtgctgcga ctgggttgga gaaaacggtg caggacattt tgttaagatg gttcataacg
     7861 gtatcgaata cggcgatatg caattgatat gcgagtcgta ccaccttatg cgcagccttc
     7921 tcggcatgac acctgatgaa atgcaccttg ttttcaagga atggaatgag ggagagttaa
     7981 acagctacct gattgaaata acaagagata ttcttgccta taaagatact gacggacagc
     8041 ctcttattga taagattctg gacacagcag gacagaaggg aacaggcaag tggacaggta
     8101 taactgccct ggagctgggc atacctctca cactcatagt agaagccgta ttttcaagat
     8161 gcctctctgc aatgaaagat gaaagagtaa ttgcttccag aattctgaag ggaccagaag
     8221 cttcgtttaa aggcagccgt gaagagtttc taaataatct gagagaggct ctctatgcaa
     8281 gcaagatagt atcatatgcc cagggttact cgctgatgag agcagctgca caggagtaca
     8341 agtgggatct caactatggc ggcatagcac ttatgtggcg gggaggatgt atcatcaggt
     8401 cagtttttct aggcaaaata aaggaagcat ttgacaaaaa tccgggactt acgaacctcc
     8461 tgctcgatcc tttcttcagg gaaaaagtgg agaaagccca gaacggatgg aggaatgttg
     8521 tagcaactgc cgcattgaac ggaatacctg tacccgcgct ctcatcggct ctcagttatt
     8581 atgatggata ccgttcggaa aaactcccgg caaacctgct gcaggcacag cgtgattact
     8641 ttggtgcaca cacttacgaa agggtggaca gaccaagggg agaattcttc catacaaact
     8701 ggacaggaag aggcggcaca acatcatcga ccacatataa tgtttagaga cattaatcca
     8761 aaaaacagcc cacaaacagt cagtcaatca acaaaaaatt aataaccaat aatttttcta
     8821 tgacagaagc agtaaaaaaa atcggcaatt acagatggac aatctgtgct cttatctttt
     8881 tcgccaccac tattaactat ctcgacaggc aggtaattgg tattctgaaa cctctgctcg
     8941 agagtgatct caacataggc gaaaaggcat actcacacat agttatggct tttcagttct
     9001 tttatgcttt cggcatgctt cttaccgggc gtcttataga taaatgggga acaaagatcg
     9061 ggtatggggt agctgttgta gtatggagtc tggcagccat gggtcatgct cttgcaaaag
     9121 gagctctggg ttttggtttc tggaggtcgt tactgggact aggcgaatcg gcaaactttc
     9181 ctgctgctaa caagggcgtg gcagaatggt tccccaagaa agaaagagcc cttgcattca
     9241 gcattttaaa ttcaggaacc aatgtgggag ccatagtcgc acctcttgct gtacctgcca
     9301 ttgtagcact atggggatgg caggcagctt ttatcattac cggtgcaata ggctttatct
     9361 ggcttttttt ctggttctgg atatatgagg taccgtcaaa aaagaagaat ctttctaaag
     9421 aggaatatga ctatatccat agtgatgtag aagagcaaaa tgagtctcag gagagtgtcc
     9481 catggctcaa actattgaaa tatcgacaga cgtggctctt cttcattggt aaggctatga
     9541 ctgatcccat ctggtggttt taccttttct ggatacccgg atggcttgca gacgtaagag
     9601 gcaccgggct taatgtgaag tcatttggcc tgccacttgc attcatctat acctcaacta
     9661 ctgtaggaag catatttggc ggatggctct catcatatat gattaaaaag ggaatgtctc
     9721 cattcagggc aagaagcata gccatgctaa tctttgcgat tctggtgaca cctatagctt
     9781 ttgcttcctc accttcaatc agcacatggg gtgcagtatg tctgatagcc cttgccgctt
     9841 cgtcgcacca ggcatggtcg gcaaacattt ttaccaccgt ctcggatgca ttcccgaaga
     9901 aggcagtcag ctccgtaacc ggtattggag gcatggcagg agctctgggc ggactcggcg
     9961 tttcatactt tgcagggttc attctcgacc tttataagaa agccggacat gttgaaacag
    10021 gctacatggt tatgttcgcc atcgcagcaa gtgcatacct tatagcatgg ctgatcatga
    10081 acgtatttgc tccaaaaaac aagaaagtag aactctgata aacacaaagc aacttatctg
    10141 atgttcagca gaaaagcccg tttaaaagat attgcagcac tggcgggagt ttcgatcgga
    10201 actgttgaca gagttctcca taacagggga caggttgcag agaagacaca ggaaagaatc
    10261 cttaaaatag cccgcgagtt aaactatact cccaacataa tggcaagggc gttgaaaaca
    10321 aagaaagtat tcactctgat gtcgctcctg ccagcacctt cagatgaaaa tgcattctgg
    10381 caaaaacatc cggagggtat ggaaaaggca atggctgaac ttaatcagtt tcctgtaaat
    10441 cttgaacagg taacctataa tttttacagt gagaccgatt tccagaaaaa ggccaaagaa
    10501 gcaataaaaa agaatcctga cggaataatt ctggctccac tattcaggtc acaatcaata
    10561 caattctgtt ctgaactgaa aaacaaaaaa ataccctttg tctttgttga caattacctt
    10621 actgaaacaa attttcttgc atatatcgga gaagatatat ttcgcagcgg cagggtggca
    10681 ggtcagcttg ccgacctgct caccgcagct gacgaggata ttcttatcgt caatattgca
    10741 aaaaacatcc ggaatatgca ccacctgaat aaaagggtga aaggattccg gagctacttt
    10801 tcaggttcag atagaatgaa gagcaaaata ttcatactga acatccctga tacaaagaga
    10861 aagactgtca aagaaatgac agacaaaatt ttatcagtcc atccaggcat aaaaaccata
    10921 ttcgtaaccg gttcaaaatc attcattatt gctgaatatg ttcattcctc aggtcacaaa
    10981 cagatgaata ttataggata tgaccttatt gataaaaatg tggagtatct cagaaaaggg
    11041 ataataagat tcattatagg acagagacca gaagaacaga cttacagggc agtaaagaaa
    11101 ctatttgatt atctttcatt taacaaactg cctgaaaaaa tggaatatct tccggttgat
    11161 attgtgactt cggaaaatgt ggacttcttt ataaacaata tttaaaaaat aatttttcag
    11221 attgatgaaa aaactaggaa aatattcaat gggtatcggc gacaggtttg gccgtcaggc
    11281 gggtgcccag ctcaaggcta taattgatgc cgggaaaaaa ggtgttgaaa ttaccccggt
    11341 ctggaacaag tcgaaccgtg agcatattat aattggtaca gaacctgctg atgtgagaaa
    11401 ggaggctgat gaagcggtca aagcagcgaa atttaaaaag ccctattttg tggatgccga
    11461 ccatatcaac ttcgatactg ttgggagatt tatcgaagct tcagatttct ttacaattga
    11521 cgttgcagca tacatagggc agagagcctg tgaagaagat ataaaagcct ttgtgaagaa
    11581 agcagcaaaa tataaaggta aactccgcat acagggtatt ccgaagccct tcaacattac
    11641 ttctgcttat cttaagaaag ttgcagagaa atatctcttt gcaacaaaga tggcaggtgt
    11701 gatctacagg aagattgaga aactaaaggg agccggcaaa tttgtaacag aggtttcgat
    11761 ggatgaggtt ccagatccac aaacgccggc agaactcttt ttcattctca tgatgctttc
    11821 cattgaaaac atcccgttac agaccattgc tcctaaattt cagggcagat tcaataaagg
    11881 ggttgattat gttggcaatc ctgaactttt tgcacttgag tttgaaacgg atcttttagt
    11941 tataagacat gctgtaaaac ttttcaaact tccggagaat ctgaagataa gtgttcattc
    12001 gggctctgat aagtttgcac tctaccctca tattggcagt ataatccgca agtatgatgc
    12061 aggaatacat ctgaagacag ccggaacaac ctggcttgaa gaggttaccg gacttgcaat
    12121 ggcaggagga gaagcactta cgtttgttaa agagatgtat aaaagtgctt atgaaaagat
    12181 tgatgaactc tgtgctccat atgccgatgt tatagacata aagaaagaaa ggctcccctc
    12241 gcctgctgaa gttatgaaat gggacagtga aaaactctca tcagcaataa ttcacgacgc
    12301 taaaaatccg aactacaacc ccgacatgag gcaactgata catgtcggat ataagcttgc
    12361 tgcactgaga atgaaagagt accttgaact gctggataaa aacaaagatg tcgtctccag
    12421 gtgtgtctac gaaaatattt acagcagaca tatctgcagg ttgtttggga taggataatg
    12481 ttactttctt ttgcttgact gaccccccac ccccctgaga ggggggcttg tggttcattt
    12541 gccagcaaca ctgtattaac tacttattta tcctcaataa ttcttcaagg gggatactct
    12601 caaacaccag atcggcctgg ctgctctcat aaagaattcc tattgttctt tcatcaactg
    12661 aagtaagaca cgaatagcct cttcctgtac cttcatcaag gagaaaataa tattcttcag
    12721 gccaggtgag tccgtcatcg aaactgactt tgatagtaat gtttcttctt ggcgggcttt
    12781 cgacatcagg gtttgaaaag accagaacat gctttttctc tccgtttaaa gagcattcat
    12841 gcttcagcag ggaagccata caaacgggtt ctctcagtgt atttcgtgat gaagtatgtt
    12901 ccctccatgt tttaccaagg tctgaagtaa ctgcgacagc ccgtcctgtc tcaccctttt
    12961 gacgctgacc gtaacgcatg ttcagcatca gggttccgtc atccaactgc acaactgcac
    13021 attcgttggt gcctgaaaag gccggattac ttgttttcca tgtcagccct ccgtcaaggc
    13081 tgtagatgat atttgaaaaa ggcactccgt tctcatccct tccctgagca gggaaaacaa
    13141 gagtgccgtc atccattgta ataccccttc ccggtgccgg agctaatagc caccactccc
    13201 gtctcttgca cattctggtc agattttccg gctcactcca cgttttgccg tcatcggtgc
    13261 ttgttgttat cagaaactgc gatgtttgcc tgacgtcaaa gccgggctgt gagcccttcc
    13321 ttctccattg atgctcccat gcattgcttt cttcggtaag tccttcaatc cagcgaccgg
    13381 cagtatcgag tacaccatgc atccacagac cagcgacaaa tattttcccg gagttttcat
    13441 caaccagaat gcaggcatca ctcaccccat tgaatttctg cggaaggcca ccccactcat
    13501 ccatatccag cactatccgc attggctccc atgtgttccc accatcagta ctgcggctca
    13561 gtccaatgtc aatatcaccc tgcaaatccc tgctgcttaa ccttcgcaca tcatatattg
    13621 ccagaagtgt gccggctttt gttgtcacca gtccaggtat tctgtaagag tgcacattgt
    13681 catcaagatg tttgcgtact gccaccccca ccctctgcct tatgccagtg tttatttcta
    13741 taacctccat ctttctgcat ccggttattc tcacagaact aaatccaaaa tcaatcctgt
    13801 tatgcaaact ggtactatgg ctcagattac acaccaccca gaaataattg tcaccagttg
    13861 agagtgcctg ctcccctttg aaatttaaaa ccctggagat cttttctgct tttccaaaaa
    13921 tgtccttaac agtatttttg cccggccaga gtgaatcaac tccggtgaaa agtgtctgga
    13981 cgttttca
//
LOCUS       DAOSNB010000030        13468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4601_length_13468_cov_4.502796, whole genome shotgun sequence.
ACCESSION   DAOSNB010000030 DAOSNB010000000
VERSION     DAOSNB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4601_length_13468_cov_4.502796"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLG42_02455"
     CDS             <1..427
                     /locus_tag="PLG42_02455"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOU30104.1"
                     /translation="EVVNEILPAEKPRYLMGVGTPENILESIALGIDMFDCVIPTRNG
                     RNGMLFTNSGIINIRNRKWADDFSPIDPEGASEVSRTYTKAYLRHLFVSGEMLGAQIA
                     SINNLSFYLNLVKEARKKIEEGNFSEWKKQIIKKVSSRL"
     gene            385..1524
                     /locus_tag="PLG42_02460"
     CDS             385..1524
                     /locus_tag="PLG42_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOU30105.1"
                     /translation="MEKTNNKKGIVKTLNRLSIKIIDRYIIRKFLGTFTFSLLMIIVI
                     AVVFDLTEKLDDFMEKEAPLRAIIFDYYLNFIPYFATIFSPLFVFISVIFFTSRMAVN
                     TEIIAILSGGMSFRRMMWPYFIASLVIAIFAFTFTNFVIPQANLKRIDFENKYYRITR
                     VKRGQVVNFHRQVYKNVYMYMERYNPEIQTGTNFTLERFDDSGRLESKLTATRVTWDT
                     TTSKWSAWTYYIRDFKDSVEVISRGLRIDTALTIRPEDFSRDPQYVGTMRYKELNDYI
                     GLLRLQGSDELKLFLIEKHRRFANPFSVFILTLIGVSLSSKKIRGGIGMQIGIGLALS
                     FSYILFMQFASQFSLKGNLDPMLAMWIPNILYLAIALFLYRLAPK"
     gene            1813..3357
                     /locus_tag="PLG42_02465"
     CDS             1813..3357
                     /locus_tag="PLG42_02465"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOU30106.1"
                     /translation="MTIEKKINELNERRKKVIAGGGAEAVEKQKAMGKKTARERIQAL
                     LDENSFHEYDMFVEHDARDFDMDKKKLPGDGVVTGTGTIHGAPVAVFAQDFTVAGGSL
                     GIMHARKITKIMDYALKMRIPLIGINDSGGARIQEGVNSLYGYGEIFFRNTLASGIIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKTVLGEEISMEDLGGARV
                     HSEISGNAHFFSKNEDECFASIRKLLSFLPWNNSEKAKLTEPAEPKLGYDIQNIVSDD
                     PTMPYDVRDIIRAVVDNSDFLEIQEMYARNIVIGFARLGGRTAGFVANQPNELAGVLD
                     VDSSDKAARFIRFCDAFNIPIITLVDVPGYLPGVDQEHAGVIRHGAKILYSYSEATVP
                     KITVILRKAYGGGYIAMASRHLRADFVFAWPTAEIAVMGPEGAANIIFRKEIASSENP
                     EETRKKMVEEYKKKFANPYVAASRGYVDAVIEPRETRKMLLHALMVTENKQVSLPPKK
                     HGVPPF"
     gene            3398..3712
                     /locus_tag="PLG42_02470"
     CDS             3398..3712
                     /locus_tag="PLG42_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOU30107.1"
                     /translation="MEEYKLLKIDDTLYRTHLSGKFLNRKKYQPVNPGAVTSFIPGTI
                     IDVFVKEGQSVKEGDVVVILDAMKMQNRLKSHLEGIVKKVHVKKGDRVPKGAVLIEVE
                     PA"
     gene            complement(3723..4538)
                     /gene="ispE"
                     /locus_tag="PLG42_02475"
     CDS             complement(3723..4538)
                     /gene="ispE"
                     /locus_tag="PLG42_02475"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOU30108.1"
                     /translation="MINFPCAKINIGLRITSRREDGYHNIQTIFYPVRLCDVLEFVIM
                     PPGSDDDRLTITGMDAGNPCENLVLKVLATLRKIYKIPYLDIHLHKVIPPGAGLGGGS
                     SDAAFFLKMINRHFGLGLNNEDLRNLALSAGSDAPFFIENLPAYAEGRGECLTSLPPL
                     PEGYHLVILYPGISISTREAYMKCTPVMREPLLPVYYREDIIRWRKLIINDFEKIVFE
                     SYPVLGELKEKLYENGALYSSMSGSGSAVYGIFSEKPELPPALSRHVIYSGPF"
     gene            4751..6277
                     /gene="dnaB"
                     /locus_tag="PLG42_02480"
     CDS             4751..6277
                     /gene="dnaB"
                     /locus_tag="PLG42_02480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOU30109.1"
                     /translation="MAKQRSGTRPVVVTLPDTGRIPPQATDMEEAVLGALMLEKDAVI
                     TVLDILKPESFYKEAHQKIFTAISNLASRDCPVDLYTVTEELRATGELESVGGPVYLT
                     QLTSKVVSAANVEYHARIVAQKYIQRELIRVSSEIQARAYDDTYDITDLLDFSEDSIF
                     QIAEGNIKREVAPIKEVLKEAIAEIEEAGKREDALVGIPSGFTKLDRITSGWQKSELI
                     IIAARPSMGKTAFALTMARNMAIEHGKKVAIFSCEMSAVQIVNRLIIAETDIPSHKIK
                     NGRLTEEEWRHLDSRIKKLMQAPIYIDDTPAITITELRAKCRRLMSQHKLDIVIIDYL
                     QLMTGPQNSGSREQEVSNISRSLKSIAKELDVPVIALSQLNRSAELRGGYKKPQLSDL
                     RESGAIEQDADIVVFIHRLDKFGILTFEDGTSTRGIADIIVAKNRNGPVDEIRLRFRE
                     EKALFVDYEDFEIEQTFESTPAPKSVTFGSKMNHDDFWSPSGRGVDYENDDFNEPPFG
                     "
     gene            complement(6294..8993)
                     /locus_tag="PLG42_02485"
     CDS             complement(6294..8993)
                     /locus_tag="PLG42_02485"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOU30110.1"
                     /translation="MLSRLIFSAIILLMFNLQASPQILKGNIKDSQGNPIPYATVYIR
                     ETRQGTVANYRGDYEIHLPEGNYSVIYQSLGYSPEVRNIKLGKTTVISDIVLQVQYYQ
                     IPEIRITATGEDPAYSIMRKAIGLAKYHLNEVSYYSADVYMKGTLVINKIPKLLQRSM
                     TSGSRNRRGSQGSSTIKEGETYLMESFNEIEFNAPDRYTQRVISSQSTFPEEGNQVSP
                     MDFIKASFYEPVIAGMAISPLSPEAFAHYRFKYEGSTEQGDNTISKIRVTPKRKSQQL
                     FEGVIYIVEDLWCIHSLSLVNENLAGTISIQQLYIPVKNNIWLPVSLKLEMDISIIGV
                     LARATYGSSIKYRAVELNSGLQKPPGIAMSQSGKPSTQQIQSDTSSSKTRAQIESILS
                     KGELSNRDMARLSKLIEKESKNSLPDSVRNSLEIKDRTTYIIEKDAGKKDSAFWASIR
                     PIPLSETEAQSIRLASNLKTELAGSVRQADTTTEKTEKKRTKFLVHTKDISFGRSWSD
                     TSGFSFSFDGLFRLKNLSFNTVDGFVYGTNFGITKRWHNSNSLSFYPEISYAFGREAF
                     MWKLNSQYRFDRMHQAQVYLRSGMTSRDMGSAGGINTFLNSVTSLLLRKNYLKLYDSR
                     YITLGHRRELINGLYIDISAGYDDRRVLENTTDFSIIKPNREYTPNIPDNYFLTLPGD
                     PPPYELKDNRHYEASLSLQWVPRQRYRIRNNAKIPAGSDYPTITLTWRHFANRFTDGI
                     STMRNYDHLRLEVSQRKETGAFSEFMWRFRTGGYIKNDNLTFLDFNHFNPQPLPILLN
                     NYEDAFRLKDYYSLSTPEFFTEFHTKYTTPYLLLKLLPALSNTLMRENVSVSFLWSDG
                     NKAYTELGYSISELLFLGEAGIYAGFDNFSFSSAGFRIILKFN"
     gene            complement(8997..9671)
                     /gene="rsmI"
                     /locus_tag="PLG42_02490"
     CDS             complement(8997..9671)
                     /gene="rsmI"
                     /locus_tag="PLG42_02490"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOU30111.1"
                     /translation="MSKLVLVPTPVGNLNDITIRAIEVLKEVDLILAEDTRHASKLLK
                     HYDIPTPVRSHHKFNEHRKIEEICSEILAGRNIALISDAGTPGISDPGFLLVRKCIEE
                     GITVETLPGPVAFIPALVSSGLPCDRFCFEGFLPQKKGRQKKLHALKDEDRTMIFYES
                     PFRLVRALEEMAGVFGHDRRACVSREISKIYEEHRRGTLAELLEHYTKHPPRGEIVIT
                     VAGKKH"
     gene            complement(9664..10386)
                     /locus_tag="PLG42_02495"
     CDS             complement(9664..10386)
                     /locus_tag="PLG42_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30112.1"
                     /translation="MDFNSTIDIILKDLKEVREIIEDFKNYPNVPVLQIELAKTKCRS
                     AEEIISLLKVLKPGFEEAERKHEKMKSSDETLIVISEEETAEKEPSAIVKTDIVTEKT
                     EAAQQVTKEEKTAPVRHQESTGKKKATTKIFADKFSGTKQTILDTFGDKKDDGISEIL
                     KSQTVRDLTEIIGVNDRFLFIREIFGGNRHAYEEVISKLNSVSSIEEAKDILKNYYSE
                     GEENEVVNQLLNLVKRKLPADE"
     gene            10502..11098
                     /locus_tag="PLG42_02500"
     CDS             10502..11098
                     /locus_tag="PLG42_02500"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOU30113.1"
                     /translation="MEFLESEIRNAGGRGSIEVITGSMFSGKTEELIRRLRRAQLAGL
                     RVEIFKPAVDRRYSDTRVVSHDEKSISSTPVDNALSIILLAGDCDVVGIDEAQFFDDS
                     LVDVCNKLADDGKRVVVAGLDMDFMARPFGPMPDLMAVAEFVTKVHAICTRCGNLAQY
                     SFRKVPVDQQVLLGEKDVYEPLCRKCYNEMKRSKSEKR"
     gene            11102..>13468
                     /locus_tag="PLG42_02505"
     CDS             11102..>13468
                     /locus_tag="PLG42_02505"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOU30114.1"
                     /translation="MKITTGKAAEITGGRLYGPDDSVIQELVTDSRLTVVQEGSAFFA
                     IKGPNHNGHRFTEQLYRRGLRIFFVEDLPENHAEYKETSFIQVKNTIVALQALASEKR
                     KHFRGEVIAVTGSAGKTVVKEWLADIIGLKEPVVRSPKSYNSQIGVPLSVWKLENNYK
                     TGIIEAGISKPGEMDKLARIIQPHTGIITNIGDAHSENFPGLKEKAAEKMKLFRDVSV
                     IVYCSDHEIIREIIHGDRNLFTKKLVDWSFKNKSSAVFVQKIKESGGVTQVSVIYGGS
                     SFGVNIPFTDRASVENALTVVAAALAHGIDPETISTGVAKLVSVAMRMEKKAGINGSV
                     LIEDYYNSDPGSLKMALEYLKSQNNRISVLILSDFLQTGRDERELYSEVAGLVKAMKI
                     DRFIGIGPALLRNRDLFPEESNFYPTTQDFCRNADLMRFKGDAILLKGARSFEFERIS
                     NLLSYRVHQTLLEISLDAISHNINEIRKRLNPGTGIMAMVKAFAYGAGAAEIASLMEF
                     HRVDYLAVAYADEGVELREAGISLPVVVMNPEPDAFDLMIKYNLEPEIYSLSLLRKFI
                     QAGQRHGLTDYPVHIKIDTGMHRYGFLPQETGELVKEISASGCLRIASVFSHLAASED
                     PAFDDFTRRQASLLEEVAGRIRDMAGYGFLVHLLNSAGIVRFPEYQFDMVRPGIGIYG
                     IAGTSGINLKQAARMKTIILQVKTVPPGEPVGYGCTDISDRERRIAVLPVGYADGLDR
                     RLGNGRGKFYIKGHFVPVVGNICMDSCMADVTGIDASEGDEVEIFGEHI"
BASE COUNT         3845 a         2693 c         3086 g         3844 t
ORIGIN      
        1 tgaggttgta aatgaaatac ttcctgcaga gaaaccacgt tatcttatgg gagttggaac
       61 tcccgaaaat atactcgaat caatagcact tggcatagat atgtttgatt gtgtgatacc
      121 gacacgcaac ggccgtaacg gcatgctgtt tacaaattca ggcattataa acattcgcaa
      181 cagaaaatgg gcagatgatt tcagtcctat cgaccccgaa ggagcctcag aagtaagccg
      241 tacatataca aaagcttacc ttcgccacct ttttgtatcg ggcgaaatgc ttggcgctca
      301 gatcgcaagc ataaataatc tgtcgttcta ccttaatctt gttaaagaag cacgaaaaaa
      361 aatagaagag ggtaattttt cagaatggaa aaaacaaata ataaaaaagg tatcgtcaag
      421 actttaaaca ggctgagcat taaaattatt gacagataca ttatcaggaa attcctggga
      481 acgtttacat tctctctcct gatgattatc gttattgcag ttgtattcga cctgaccgaa
      541 aaactggacg atttcatgga gaaagaagct ccgttgcgtg ctataatttt tgattattac
      601 ttaaatttca ttccatactt tgccacaatc ttctcacctc tgtttgtctt tatttctgtt
      661 atttttttca catcaaggat ggctgtcaac actgaaatta ttgccattct aagcggggga
      721 atgagtttca gacgcatgat gtggccatat tttattgcct cactggtgat tgctatcttc
      781 gcttttacat tcaccaattt tgtcatccct caggcaaatc ttaagagaat tgacttcgag
      841 aacaaatatt accgaattac aagggttaag agaggtcagg tggtgaattt ccacaggcag
      901 gtgtacaaaa atgtctacat gtacatggaa agatataatc ctgaaattca gacaggtaca
      961 aatttcactc tcgaaaggtt tgacgatagc ggccggcttg agtcgaaatt aactgccaca
     1021 cgtgtaacat gggataccac cacatcaaaa tggagtgcat ggacatatta cataagagat
     1081 tttaaggaca gcgtggaggt tatcagcagg ggattgagga ttgatacggc acttaccata
     1141 aggcctgagg atttctcaag agatcctcaa tacgtaggga cgatgagata taaagagctg
     1201 aatgattata taggactcct ccggcttcag ggttctgatg aactgaaatt gtttctgata
     1261 gaaaaacacc gcaggtttgc aaatcccttt tcggttttta ttttgacact catcggggta
     1321 tctctttcat cgaagaaaat taggggaggc ataggaatgc agataggtat agggttggca
     1381 ctcagttttt catatatact tttcatgcag tttgcatctc aattctctct gaaaggaaat
     1441 ctcgacccga tgctggccat gtggataccc aatatactct atctggcaat tgcacttttc
     1501 ctttaccgcc tcgcaccaaa ataataaaaa gtacttcgtc ctgaaaaagt tgaatctatt
     1561 tttccacccc ctgatccccc ttttttgaat atacccccta atccccctgg cagggggact
     1621 tatcacatgc aatgaaatag tataacagga gaagaaaaaa gtttggaaac ttatatcagt
     1681 acaggaaaag gttttgattt caaaaaaaat ggattttcat ggtttaacat gataaatatt
     1741 tttcttttag aactttaaaa gttttaaaat tgctgcctta aaaaagtaag ccggacttaa
     1801 aaactatgac ttatgactat tgaaaaaaag attaatgagc tcaatgagag gcgcaaaaag
     1861 gttattgccg gaggtggggc tgaagctgta gagaagcaga aagcgatggg taaaaagact
     1921 gcccgcgaac gtatccaggc acttctcgat gagaattcat ttcatgaata cgacatgttt
     1981 gtggagcatg atgcccgcga ttttgacatg gacaaaaaga agcttcctgg agatggtgtc
     2041 gttacaggta cgggtactat acacggcgct ccagtagcag tttttgcaca ggatttcacg
     2101 gtagcaggcg gttcacttgg aataatgcat gcaaggaaaa taactaaaat catggattat
     2161 gctttgaaga tgcgcatccc tctgattgga atcaacgatt caggtggtgc aagaatacag
     2221 gaaggagtga acagccttta cggatacggt gagatctttt tccgtaacac tcttgcaagc
     2281 gggataatcc cccagatatc tgttattctt ggtccgtgcg ccggaggtgc agtctattca
     2341 ccagccctta ccgattttgt gtttgttgtg gataagatat ccaagatgtt tattaccgga
     2401 ccggaagtga ttaagacagt actcggtgaa gagatttcca tggaagacct cggtggggca
     2461 agggtgcaca gcgaaataag tggcaatgct catttctttt caaaaaacga agatgaatgc
     2521 tttgccagca taagaaaact tttgtcattt cttccatgga ataattcaga aaaggcaaaa
     2581 ttaactgaac ctgctgagcc gaagctgggg tatgacattc agaatattgt ctcggatgac
     2641 cccaccatgc cttatgatgt gcgtgatata atacgtgctg ttgttgacaa ttctgatttt
     2701 cttgagatac aggagatgta tgcaagaaac atcgttatag gttttgcgag actgggaggc
     2761 cgcactgccg gctttgttgc aaaccagccc aatgaactgg ctggtgttct tgatgttgat
     2821 tcgtcagata aagctgcccg ttttatacgc ttttgcgatg cattcaacat acccattatc
     2881 acactggttg acgttcccgg ctatcttccc ggtgttgatc aggaacatgc gggagtaata
     2941 cgccatggtg caaagatcct ttactcatat agcgaggcaa cagttccgaa gattacggtt
     3001 attttgcgta aagcatacgg tggaggctac attgcaatgg catcgcgcca cctgagggcc
     3061 gattttgtat tcgcatggcc caccgccgaa atagcagtga tgggacccga aggagctgca
     3121 aacattatat tcagaaagga aatagcttca tcggaaaacc ccgaagaaac tagaaaaaag
     3181 atggtggaag aatataaaaa gaagtttgca aatccctatg tggctgcttc gcgaggttat
     3241 gtggatgctg tcatcgaacc gcgggaaaca cgcaaaatgc tccttcatgc tctcatggtg
     3301 actgaaaaca aacaggtatc actgccgcct aaaaaacatg gcgtgccacc gttctgatta
     3361 tcaacgatat gcaggtaaat gaaagatatt gaataagatg gaagaatata agttacttaa
     3421 aatagacgat accctttacc ggacccatct gagcggaaaa ttcctcaaca ggaaaaaata
     3481 ccaacctgta aatccaggag cagtaacaag ctttatccct ggaacgataa tagatgtgtt
     3541 tgtaaaagag ggtcagagtg tgaaagaggg ggatgttgtg gtgattctcg atgccatgaa
     3601 gatgcagaac aggttaaaat cacaccttga aggtattgta aaaaaggtgc atgtaaaaaa
     3661 gggtgaccgt gtgcctaaag gtgctgttct tattgaggtg gaaccggcat gagagtccgg
     3721 tttcagaaag gtccagaata aattacatgt cttgacagtg ctggaggtaa ttcaggtttt
     3781 tcactgaaaa tgccgtaaac tgcagatccg cttcctgaca ttgaactgta tagtgccccg
     3841 ttctcgtaga gcttttcttt taattccccg agcacagggt aactttcaaa aacaatcttt
     3901 tcaaaatcat taataatcag ttttctccac ctgatgatgt cttccctgta ataaaccggt
     3961 aacagcggtt ccctcataac tggagtgcat ttcatatatg cttcccttgt gcttatgctg
     4021 atcccgggat atagtatcac caggtgatac ccttcaggta atggaggcag agatgtgaga
     4081 cattctcccc ttccttcggc ataggcagga agattctcaa taaaaaaagg tgcatcgctt
     4141 ccggctgaaa gtgcaagatt cctgagatct tcattgttaa gtcccagtcc gaaatgtctg
     4201 ttaatcatct tcagaaaaaa ggctgcatcg gaagaaccac cccccagtcc tgcgcccggc
     4261 ggtataacct tgtgcagatg aatatcgaga tagggtattt tataaatttt gcgcaatgtc
     4321 gcaagcactt tcaataccag attttcgcat ggattacctg catccatgcc agttattgta
     4381 agtctgtcat catctgaccc tggcggcatg attacaaact ccagcacatc acataacctg
     4441 acaggataaa atatcgtctg aatattgtga taaccatcct cacggcgaga cgttatccgc
     4501 aaccctatat ttatttttgc acaaggaaaa tttatcatgc caataaattt taatgtaaaa
     4561 atatcaatat gcattataac atagaactat tcctgatttt tttctctttt taacaagttt
     4621 tgaactgaac atgtttgtaa taaaacctct atttttacaa actgtttcat tcaaattttt
     4681 acttttgaag aagacagaaa gccagctatg attttatcct aacaagttgg caatcattaa
     4741 attaacaaca atggcaaaac agagaagcgg gacgagacct gttgtggtca ctttgcctga
     4801 cacaggcagg ataccacctc aggcaacaga catggaagag gcggtgctgg gagcactgat
     4861 gcttgagaaa gatgcagtta ttacagtgct cgacattctc aaacctgaaa gcttttataa
     4921 agaagcgcac cagaagatat ttactgccat aagcaatctg gctagcaggg attgccctgt
     4981 tgacctgtac actgtaacag aagaactcag ggctaccggc gagcttgaaa gtgtgggagg
     5041 acctgtttat cttacccagc ttacttcaaa agttgtttca gctgcaaatg tcgaatatca
     5101 tgcacgcata gtagcgcaga aatacattca gagggaactt atcagagtat catccgagat
     5161 acaggcaagg gcatacgatg atacatacga tataactgac ctgctcgact tttctgaaga
     5221 ttcaattttc cagatagctg aaggcaatat taagcgcgag gtcgcgccca taaaggaggt
     5281 actgaaggag gctatagccg agattgaaga ggcaggaaaa agggaagatg ctcttgttgg
     5341 tataccttcg gggttcacaa aacttgacag aataacttcc ggatggcaaa aatcagaact
     5401 tataattatt gcagctcgtc cttcaatggg taagacagcc tttgcactta cgatggcacg
     5461 caatatggct attgaacatg gaaagaaggt ggcaatattt tcgtgcgaga tgtcggctgt
     5521 gcagattgtg aaccgtctta ttatagctga aaccgacata ccatcgcata aaatcaagaa
     5581 cggaaggctt acagaagagg aatggaggca tcttgattca aggataaaaa agctgatgca
     5641 ggctcctatt tacatcgacg atactcctgc aataacaatt acagaactga gggcaaaatg
     5701 ccgccgcctg atgtcgcagc ataaactcga tatagtaatt attgactatc tgcagctgat
     5761 gacaggccct cagaattcag ggagccgtga acaggaagta agtaatattt caaggtcgct
     5821 gaaaagtatc gccaaggaac tcgatgttcc tgttattgcc ctctcgcagc tgaaccgttc
     5881 agcagaactg agaggaggat acaaaaaacc acagctttca gacctcaggg aatcgggcgc
     5941 aattgagcag gacgctgata tagtggtatt tatacacaga cttgacaagt tcgggattct
     6001 gacattcgaa gatggtacct caacacgcgg aatagccgat attatagttg caaagaaccg
     6061 taatggtcct gtggatgaaa tacgcctcag attccgcgag gagaaggcac tgtttgttga
     6121 ctatgaggat ttcgaaatag aacagacatt cgaaagcaca ccggcaccaa aaagcgttac
     6181 cttcggttca aagatgaacc acgacgactt ctggagccca tctggaagag gggtggatta
     6241 tgagaacgat gatttcaacg aaccaccttt cggttaatcg ataaactaca gatttagtta
     6301 aacttcaaga ttattctgaa gcctgccgaa ctgaatgaga aattgtcaaa accggcatag
     6361 ataccggctt cacctaaaaa caaaagttcg ctgatgctgt agccaagctc ggtgtatgcc
     6421 ttgtttccat ctgaccacag aaacgaaacg ctgacatttt ctctcatcag agtattgcta
     6481 agagcaggca gaagtttgag aagcaggtag ggagtggtat attttgtgtg aaactccgtg
     6541 aagaattcag gagtgctcag cgaataataa tccttcagcc tgaatgcgtc ttcataattg
     6601 tttaacagta tcggtaaagg ctggggattg aaatgattga aatcgaggaa agtgagatta
     6661 tcattcttaa tatacccgcc tgttctgaac ctccacatga actcgctgaa ggctcctgtt
     6721 tcttttctct gtgatacttc aagccttaag tggtcgtagt ttctcattgt tgatatcccg
     6781 tcggtaaacc tgtttgcaaa atgcctccag gtaagcgtaa tagttggata gtcagaccct
     6841 gccggtatct ttgcattatt tcttatcctg tatctctgcc gtggcaccca ttgcaacgat
     6901 aaagaagcct cataatgcct gttgtctttt aactcgtaag gaggaggatc acccgggagg
     6961 gtgaggaaat agttgtcggg aatatttggc gtatattcac ggtttggctt gataattgaa
     7021 aaatctgttg tgttttcaag aactcttctg tcatcgtaac cggcgctgat atcaatatat
     7081 agcccgttta taagttccct cctgtgaccc aatgtaatat acctgctgtc gtaaagcttg
     7141 aggtagttct ttctgagaag gagtgatgtt acagaattca ggaaagtgtt tataccgcca
     7201 gcacttccca tgtctctgct tgtcattcct gaacgcagat atacctgtgc ctgatgcatc
     7261 ctgtcgaagc ggtactggct gttcagtttc cacataaatg cctcccggcc gaatgcataa
     7321 cttatttcag gataaaacga aagcgaattt gaattatgcc atcttttcgt gatcccaaaa
     7381 tttgtaccgt acacaaaacc atcaacggta ttgaaactta aattcttaag cctgaagagt
     7441 ccgtcgaaag aaaagctaaa tcctgatgta tcagaccatg aacggccaaa agaaatgtct
     7501 tttgtatgta ctaaaaactt agttcttttc ttttcagttt tttctgtagt agtatcggct
     7561 tgtctaactg aaccggcaag ttctgtcttc aggttagatg caagtctgat gctctgtgcc
     7621 tctgtttctg aaagcgggat gggacggatg ctcgcccaga aagcgctatc tttttttcct
     7681 gcatcttttt cgattatata tgtggtgcga tcttttatct caagagaatt tctgacactg
     7741 tcaggtaaac tgtttttcga ttctttttca atgagcttcg aaagtcttgc catatccctg
     7801 ttcgaaagtt cacctttcga taatatggat tctatctgtg ccctggtctt tgaagaagag
     7861 gtatcggact gaatctgctg ggtggaaggt ttcccgctct gactcatagc aatgcctggg
     7921 ggcttttgca gaccggaatt cagttcaaca gcccggtatt ttatggaact accgtaggtt
     7981 gccctggcaa gcactccaat aattgaaatg tccatttcaa gttttagact caccggcagc
     8041 catatgttgt tcttcacagg gatataaagt tgctggattg aaatcgtacc ggccaggttt
     8101 tcatttacaa gactcagact gtggatgcac caaaggtctt ccacaatata gattacgcct
     8161 tcgaacagtt gctgactctt tcttttaggg gtgactctta tcttacttat ggtgttatca
     8221 ccctgctcag tggatccttc atatttgaac ctgtaatgtg cgaatgcttc gggcgaaagg
     8281 ggtgaaattg ccatgcctgc tatcacaggt tcgtaaaaac tggctttaat gaaatccatg
     8341 ggtgagacct gattcccttc ttcaggaaaa gtgctctggg atgatattac tctctgggta
     8401 tacctgtcgg gtgcattaaa ttcaatttca ttgaacgact ccatcagata tgtctctcct
     8461 tcctttattg ttgaactgcc ctgagaaccc cttctgtttc gtgagccaga tgtcattgaa
     8521 cgctgcagga gtttgggaat tttgtttatc acaagcgttc ccttcatata aacatccgca
     8581 ctgtagtaac taacttcatt aagatggtac tttgcaagtc cgattgcttt tctcataatg
     8641 ctgtaagccg gatcctcacc tgtggctgtt atccttattt cggggatctg gtaatactgt
     8701 acctgcagaa caatgtctga tataactgtg gttttcccga gtttaatgtt tctgacttcg
     8761 ggtgaataac ccagactctg gtaaataacg gaatagtttc cttcaggaag gtggatttcg
     8821 taatcacccc tgtaattggc aacagttccc tgccgtgttt cgcggatata aaccgtggca
     8881 tagggaatag ggttgccctg tgaatccttt atattgcctt ttaaaatctg aggtgatgcc
     8941 tgcagattga acatcagtaa gataatggct gagaagataa gacgggaaag catttttcag
     9001 tgcttttttc cagcaaccgt tataactatt tcacccctgg gcggatgctt ggtgtagtgt
     9061 tcgagcagtt ctgcaagggt tcccctccgg tgttcttcgt atatcttgct tatctcgcgc
     9121 gatacgcagg cccggcggtc gtgtccgaaa acgcctgcca tctcttcgag tgccctgacg
     9181 agacggaacg gtgattcata aaaaatcatt gtgcgatctt cgtctttcag tgcatgaagt
     9241 tttttctgcc tccctttttt ctgaggcaga aaaccctcga agcagaacct gtcgcatgga
     9301 agccctgaac tgaccagtgc aggaatgaaa gctacaggtc ctggcagtgt ctcaacggtg
     9361 attccttctt cgatgcattt ccgcacaagc agaaaacccg ggtcggaaat gcctggtgta
     9421 ccagcatccg aaatcagggc tatattcctg cctgcaagta tctccgagca gatttcttca
     9481 atcttccggt gttcgttgaa tttgtgatgc gacctcacag gggtaggtat atcgtaatgc
     9541 ttcaacagtt tggaagcatg tctggtatct tcggcaagaa taaggtcaac ctccttaagc
     9601 acttcaattg cccggatggt gatatcattc aggttaccta ccggagtggg gacaagaacc
     9661 agcttactca tcagccggca gtttacgttt tacaagatta agaagttgat tcacaacttc
     9721 attttcttct ccttcagaat aataattctt cagaatatct tttgcctctt ctattgaaga
     9781 aactgaattg agttttgaaa ttacttcttc gtatgcatgg cggttgccac caaatatctc
     9841 tctgataaac agaaatctat cattaacacc tataatctca gttagatccc tgacagtctg
     9901 gctcttaaga atttctgaga ttccatcatc ttttttatca ccgaaagtat cgaggatagt
     9961 ctgttttgtg ccgctgaatt tgtcggcaaa tatttttgtg gtggctttct tttttccggt
    10021 cgattcctga tgtcttacgg gagctgtttt ctcttccttt gtaacctgct gtgctgcttc
    10081 agttttctct gtcacaatat ctgtcttaac tatggcagaa ggttcttttt ctgcagtttc
    10141 ttcttcggag attacaataa gagtttcatc agaactcttc attttttcat gtttcctttc
    10201 agcctcttca aaaccgggtt tgagaacctt cagtagtgaa ataatttcct cggcactcct
    10261 gcattttgtt tttgcaagtt cgatctgaag aaccggaacg ttagggtaat tcttaaaatc
    10321 ttctattatc tcccttactt ctttcaggtc tttcagtata atatcgatag ttgaattgaa
    10381 atccattgtt tgctatttat tatttgaaat gccaattttg tttactttgc aaaagtaatc
    10441 ataattattc aagtattacc agccggaaaa gggcttgttt aaactattaa caatcttgaa
    10501 tatggagttt cttgagagtg aaatcaggaa tgcaggaggg aggggaagca tagaagttat
    10561 taccggctcg atgttttcgg gtaagactga ggaattgata agaaggctca gacgtgcaca
    10621 gctggcaggg ctcagggtgg agattttcaa acccgcggtt gaccggcgct attccgacac
    10681 cagggtggtt tcgcacgatg aaaagtcaat aagctcaact ccggttgata atgctttgtc
    10741 aattattctc cttgcaggcg attgcgatgt tgtgggaata gatgaagcgc agtttttcga
    10801 cgattcgctt gtggatgttt gcaataaact tgccgacgac ggaaagaggg ttgtcgttgc
    10861 agggcttgac atggatttta tggcaaggcc ttttggacct atgccggatt tgatggcggt
    10921 ggcagaattt gtcacaaaag tacatgctat atgcacccgt tgcggaaacc tggcgcaata
    10981 ttctttcagg aaagtaccgg tggaccagca ggtgctgcta ggcgaaaaag atgtgtacga
    11041 accactgtgc agaaaatgtt acaatgaaat gaagagaagt aaatctgaaa aaagataagc
    11101 tttgaaaatc acaacaggca aagcggctga gattacaggt ggcaggttgt acggccctga
    11161 tgattcagtc attcaggaac tggttactga cagccgcctc accgtagttc aggaaggttc
    11221 agcatttttt gcaataaaag gaccaaatca caatggacac aggtttacag agcagttgta
    11281 ccggcgggga ctcaggattt tttttgttga agatttgcct gagaaccatg cagagtataa
    11341 agagacctca tttattcagg ttaaaaatac aattgtagct cttcaggcac ttgcatctga
    11401 aaagagaaaa cacttcaggg gagaagtgat agctgttacc ggtagtgcag gcaaaacagt
    11461 tgtaaaagag tggcttgccg atattatcgg acttaaagag ccagttgtaa gaagcccgaa
    11521 aagctataac tcacagatag gagtgccgct ttcggtatgg aagctggaaa acaattataa
    11581 aaccggcatt attgaagcag gtatatcgaa acccggagag atggataagc ttgcccgcat
    11641 cattcagcca cataccggta tcattacaaa tataggtgat gcacacagcg aaaatttccc
    11701 gggactgaag gaaaaagctg ccgagaaaat gaagcttttc cgcgatgttt cggttattgt
    11761 ttattgcagc gatcatgaga ttatcaggga aatcattcat ggtgacagga atttatttac
    11821 taaaaaatta gttgactggt catttaaaaa taaaagttct gcggttttcg ttcagaaaat
    11881 aaaagaatcg ggtggggtta cgcaagtaag tgtgatatac ggtggctctt cattcggagt
    11941 taatattccg tttactgaca gggcatcggt tgaaaatgct cttacggttg tggctgccgc
    12001 acttgcgcat ggtattgacc cggaaacaat aagcacaggt gttgcgaagc tggtgtcggt
    12061 tgccatgagg atggagaaaa aggctggaat aaacggctcg gtgctcattg aagactatta
    12121 caattctgac ccgggttctt tgaagatggc gctggaatat ctgaagtcgc agaacaacag
    12181 gattagtgtt cttattctgt cggatttttt gcagacagga agggatgaac gagagctata
    12241 tagtgaagtg gcagggcttg ttaaagcaat gaaaattgac cggtttatag ggattgggcc
    12301 tgctctttta cgtaacaggg atctttttcc ggaggaaagc aatttttatc cgacaacaca
    12361 ggatttttgc agaaatgctg atcttatgcg ctttaaaggt gatgccatac tgcttaaagg
    12421 agccagaagt tttgaatttg agcggataag caatctgttg tcgtacaggg tccatcagac
    12481 actccttgag ataagtctgg acgccatttc gcataatatc aatgagataa gaaaaaggct
    12541 caatcccgga acagggatta tggcgatggt caaggctttt gcttatggtg cgggggcggc
    12601 agagattgct tcgcttatgg agtttcaccg ggttgattat ctggctgtgg cgtatgctga
    12661 cgagggtgtc gaactgcggg aggcaggcat atctctaccg gtagtggtaa tgaatccaga
    12721 accggatgca ttcgacctga tgataaaata taatctcgaa ccagagattt acagcctttc
    12781 gctgctgagg aaatttattc aggcagggca acggcacggg cttactgatt accctgtgca
    12841 tataaagatt gataccggaa tgcaccgtta tggatttttg ccacaggaga caggtgaact
    12901 tgttaaagaa atatcagctt ctggttgtct aaggattgct tcggtttttt cacatctggc
    12961 tgcatccgaa gaccctgcat tcgacgattt tacccgtcgc caggcatcgc tgcttgaaga
    13021 ggttgccggc aggatacgtg atatggcggg atacggtttt cttgttcatc tgctgaatag
    13081 tgctggaata gtaagattcc ctgaatatca gttcgatatg gtacgacccg gcattggtat
    13141 ttatggcatt gcgggtacct cagggataaa cctgaaacag gcagcacgta tgaagacaat
    13201 aattcttcag gttaaaacag ttccacctgg cgaacccgta ggttacggtt gtacagatat
    13261 ttccgacagg gagcggcgta tagcagttct tccggtaggc tatgccgacg ggctcgaccg
    13321 caggctagga aacggaagag gaaaatttta tataaaaggt cattttgtac ctgttgttgg
    13381 caatatctgt atggactcat gcatggctga tgtgacaggc atagatgctt ctgaaggtga
    13441 tgaggtggaa atttttggcg aacatatt
//
LOCUS       DAOSNB010000031        13141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4748_length_13141_cov_3.923812, whole genome shotgun sequence.
ACCESSION   DAOSNB010000031 DAOSNB010000000
VERSION     DAOSNB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4748_length_13141_cov_3.923812"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLG42_02510"
     CDS             <1..763
                     /locus_tag="PLG42_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU30115.1"
                     /translation="ATGRQAFVTEAQTTAASAQSSYERSVEQLEKIFTDAVAYAAVEK
                     PEKTNLRLEAMRGLFNGSKTLFINAQDAKGIIAAVSFAKRYGVKKIVLANATESAWEV
                     KDFLKENNIPVIVANPLSLPLYEHSDVRMPFKLAAMFMKEGILTGLTYSSMAYGNLPF
                     AAGQTVAYGLTKEEALQTVTLNNAKILGIDDVTGSLEPGKDANIVVSTGDLLDMKTNN
                     VELAFITGRNISLDNKHKQLTRRFKEKYNNVNSGN"
     gene            1007..2158
                     /locus_tag="PLG42_02515"
     CDS             1007..2158
                     /locus_tag="PLG42_02515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU30116.1"
                     /translation="MKIIIAGTAYPFRGGLAAYNERLARQFQSEGHDVSIITFTTQYP
                     SFLFPGKSQFSSAPAPSDLKIERRMSSVNPFTWIINGLKIKKTRPDILIFKYWLPFMA
                     PCFGTIARMAKSNRHTKIIAIFDNVIPHEKRPGDTFLTNYFVKSVDGAIVMSETVKTD
                     LLKFRSNIPVYLTPHPLFDNFGQKIEKKEALTRLGLDPSFNYILFFGFVRPYKGLDIL
                     IKAFKWVREEYTETKLIVAGEFYEDEKPYRKLIEEEKLQNDIFLFNRFINDDEVALFF
                     SACDLVVQPYKSATQSGVTQIAYHFEIPMIVTKVGGLKEIVPGGKCGYVVEPDPIEIA
                     DAITRFYSERNSIDFTSYIREEKKKYSWDKMTAVILDIYRKISEDDSKK"
     gene            2142..3164
                     /locus_tag="PLG42_02520"
     CDS             2142..3164
                     /locus_tag="PLG42_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOU30117.1"
                     /translation="MIVRSKAPLRLGLAGGGTDVSPYSDIFGGAILNASINLYAYASI
                     EPGDNGEITLRLEDKNLELRYPLTERLEPDGNMDLIKGVYNRIIREFRLSPQPFRLSA
                     YVDAPPGSGLGSSSTLVVAVLGAFAEWFRLPLGEYDLAWLACDIERNDLGMAGGKQDQ
                     YAATFGGVNFMEFYKNDKVIVNPLRIKEQILNELSNNIVLYYTETSRISSSIIENQQR
                     NVKEGVSESVEAMHMLKELAISMKEALLKGELDSLGELLDNSWNYKKKMAGGITNPHI
                     DTIYDTALKAGATGGKISGAGGGGYMFFYCPRNTRYNVIRALKQFGGHEQKYEFTKRG
                     LETWTI"
     gene            3175..3873
                     /locus_tag="PLG42_02525"
     CDS             3175..3873
                     /locus_tag="PLG42_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU30118.1"
                     /translation="MIKEAVILAGGLGTRLRGVVDDLPKPMAPVRGQPFLTWLFDLIE
                     KQGVEKVVLATGYKHEKISSFFDFRYKNLNIEYSVEAEPLGTGGAVLMALQKIKGENF
                     FLLNGDTYFEVNFHDFEFFFNSTGSVMSVALHPVKNAARYGTVILQENRIVAFGEKTA
                     NRGGLINGGIYVVNKKWFEENAPGEKFSLEKDVLEKKISDNLITGYVSDAYFIDIGIP
                     EDYYRAGEELPPVG"
     gene            complement(3877..4830)
                     /locus_tag="PLG42_02530"
     CDS             complement(3877..4830)
                     /locus_tag="PLG42_02530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU30119.1"
                     /translation="MDLSVVIPVYNEEESLPELAEWIVRVCRNSGFTYEIIFIDDGSS
                     DSSWKVIGDLCRSNNNIKGVSFRRNYGKAAALHTGFGLASGDVVITMDSDLQDSPDEI
                     PELYRMVKEEGYDLVSGWKKKRYDPFVKRVTSRFYNFTAKVWSGIRLHDFNCGLKAYR
                     NEVIKSIEVFGEMHRYIPILVKEAGFKKIGEKVVQHRPRKYGTTKYGLDRFIKGYLDL
                     LTIGFITRFGKSPMHLFGLLGTLMFLAGFLMAGYLGGRKLWYVSHNMRAPLVTDSAWF
                     YIALTVMVIGTLLFLTGFIGELVNRNSPERNKYLLKDKINA"
     gene            complement(4840..5373)
                     /locus_tag="PLG42_02535"
     CDS             complement(4840..5373)
                     /locus_tag="PLG42_02535"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOU30120.1"
                     /translation="MEEKISVWKANLTNGLIFGLIYIVYTLIMYFLDLSLNRTQGYIF
                     LAAEVIILYLMVKSYRDSYLHGYIKYGQSVGAGVVIFLYFSVIAALFTYILYKFIDPG
                     LIDKQLEMAEEMLARRGMSQEMIDTSMNIQKKILVPEFIAPASIINNMLLGTIMSLLV
                     SIFTRKEGNPLIDIPEN"
     gene            5471..5570
                     /gene="ffs"
                     /locus_tag="PLG42_02540"
     ncRNA           5471..5570
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLG42_02540"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5679..6293
                     /locus_tag="PLG42_02545"
     CDS             5679..6293
                     /locus_tag="PLG42_02545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOU30121.1"
                     /translation="MLLKIHPENPGTIQIRKVVEVLEKGGVIICPTDTVYAMGCDIKA
                     THAIEKIARFKGTDPKNPDMSLIFHDMSQLSEYTIIKDNNIFKLLKRNLPGPFTFIVK
                     ANNQIPKMFRNRKKTVGIRIPKNNIILEIVRELGRPLITTSIHDRNEIIEYTTDPELI
                     YERYRDMVDLVINGGYGSNEPSTVVDCTGEEPVIIRQGLGTLEF"
     gene            complement(6302..7708)
                     /locus_tag="PLG42_02550"
     CDS             complement(6302..7708)
                     /locus_tag="PLG42_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOU30122.1"
                     /translation="MLIFEGIMMLAAAGISYFLKDPAASSLLFSGLITITAGVFVYTP
                     VKGDEQLYSNREGFLIITGTFLIYIITGMLPYLLSGTIKNITDAFFESASGFTTTGAT
                     ILNSIDSLPRGIVLWRSLTQWIGGLGIIFLSLYVLPVFKDNIIQLSTTDFSGQPQDKI
                     YPRWVDTAKRLISLYIILTILEALFLAAGGMNLFDAACISLSTLSTGGFSPHNYSLTL
                     LTAPYAKIVITIFMFLGGTNMAIIYYLYKRNLQKIKENREFIYYLVLSLAFCLVVSGL
                     LMVQKHPFSESVINGSFHVISIITTTGFYTNDYTQWGNFILLLLFILMFTGGTVGSAS
                     GGIKIARLMTLGINTRMEIKRLIHPDAYLPVRINRKIIPQEIVYNILVFVTIYFIIIC
                     SSAFIFSMMNYDIITSFSTAASILANIGPAIGEFGPLTTYSSLPVAGKWFMSFLMILG
                     RLEMLAVMVLFARSFFRK"
     gene            complement(7754..9094)
                     /gene="trkA"
                     /locus_tag="PLG42_02555"
     CDS             complement(7754..9094)
                     /gene="trkA"
                     /locus_tag="PLG42_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOU30123.1"
                     /translation="MRIIIAGAGEVGTHLAKMLSNENHEILIIDPDEERLKPIDSTLD
                     VLTLHGSATSVSLLQNAMKKKTDLFIAVTHSEDTNITASILARRLGAIKTIARIDNLE
                     YLNRENEEFFKSLGIDSLIYPELIAAREVLMLLHETGTTDFMEFSGGQLVMYVQKLDE
                     KAPILNKSLKEIAKLYKTDQYRAIAIKRNEKTIIPSAEEVFQPGDVVYLISTREGIDE
                     MMKTSGKESFEAKSIMIVGGSRIGKHVALHMQKTSAVKLIDSDYEKCQALAELLEQTL
                     IINGDCRDADLLSREGIGNMDCFVAVTGNSETNILSCLLAKKMGVKKTIAEVENTEYI
                     HLAENIGIDTIINKKIAAASRIFRYTTNPNVTQVKYIAGAEGEVVEFIVPVNARITKG
                     TLRSIDFPDEAIVGGGTRDGVPFIATGDTIIKANDRVVVFTLPSAFEKVSKFFI"
     gene            complement(9165..11174)
                     /locus_tag="PLG42_02560"
     CDS             complement(9165..11174)
                     /locus_tag="PLG42_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOU30124.1"
                     /translation="MLFKEIPFLRIFIPYCLGIAASFCAEGIGIKIMLLLSVFLIIPL
                     LISYFTIKSYNNTAFGAILTAALFLSGVILAVNERRSLSTLEQKEQLFCSVVLDFPEE
                     KENSFLIKSKLKYIWSDSVKKRIEGSIILYHSKNSQLTKLMPGDIIMFRTTPLEIRNK
                     GNPYEFDYRFYMESKGFRYYGFIQKDDIIYYGSPSRRTLREKALIFREQIINMYQEKG
                     ARGKRLALAAAITLGEKSMLGDDEKEHFSRAGVMHIMAVSGLHAGVLSLFIFNILFFL
                     KGKLKILRVFFTIATLWFFAFTTGLSPSVMRASLMFSFLHTGQLLNRKINPLNSLLAS
                     AFILSVINPLVIFDPAFQLSYVAVFFIILFYRDFYNTLTFNNFMSDRIWQLISVSFVA
                     QAGTLPLTLAMFNRFSLIFLLSNILIVPLAAVIIISGFLAVITAPVGFVASIFVTVMD
                     RTSFLAGYLTEKTSMLPFSSLENTGITVPESILLIIALAIFLNYLLSEKKKSVVLPLA
                     AFLALIVTVTTKKVISGFKNELIVYNTQSVPVAGIKNGNTLNLFYMGEKVPGEALRHS
                     AITGLHVKTIDVRGKMPFLIRAGGKNILISDSYPAENAVKKSDIVIYTGLRPLFLSES
                     AKPDNIILTSVYPGRVFENKVRNNRVNGLWFIKSQGCFRMKLKRE"
     gene            complement(11214..11780)
                     /gene="def"
                     /locus_tag="PLG42_02565"
     CDS             complement(11214..11780)
                     /gene="def"
                     /locus_tag="PLG42_02565"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOU30125.1"
                     /translation="MIYPVVVYGHPVLKKVAAEVERNYPELKQLIDDMFETMYAAEGV
                     GLAAPQIGKSIRVFVIDGAPLAEDEPEFANFKRVFINPKIIEKDGELVPMNEGCLSIP
                     NIREEVKRESHIRVQYYDENWEFHDETFEGYKARVFLHEYDHLDGILFVEKINPLRKR
                     LIKGKLNDISKGKFEADYKTIVYTPRKK"
     gene            complement(11785..12216)
                     /gene="ruvX"
                     /locus_tag="PLG42_02570"
     CDS             complement(11785..12216)
                     /gene="ruvX"
                     /locus_tag="PLG42_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOU30126.1"
                     /translation="MGRILAIDYGKKRVGLAVTDPMRIIASPLETVPVNEVENFLSDY
                     FKKETVDEIVIGYPLQMNNEPSEAVKYIEPFIRRMRKLYPDKTIYLADERFTSVIGQQ
                     TLIEGGVKKKRRMDKSLVDKISASLILQSFLEKRSFLNSKK"
     gene            complement(12298..>13141)
                     /locus_tag="PLG42_02575"
     CDS             complement(12298..>13141)
                     /locus_tag="PLG42_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU30127.1"
                     /translation="ALSNGRVNSETKAAFEEWLASRNNAEASKVASAKVVEACEKDGS
                     EVAREILSLKQYLVKKSHWIIGGDGWAYDIGFGGLDHVLAQGEDVNLLVLDTEVYSNT
                     GGQASKSTPAAAVAKFAAAGKKIRKKDLGLMAISYGYVYVAQVAMGANNAQLLKAVKE
                     AEAYPGPSLIIAYAPCINHGLKYGMGKSQEQEERAVKAGYWHLYRYNPLLEAEGKNPF
                     ILDSKEPDWSLFQEFLHSEVRYTALLKTNPKEAEALFKAAEENAKWRYKTYKRLAEMQ
                     YPQQ"
BASE COUNT         3969 a         2750 c         2562 g         3860 t
ORIGIN      
        1 agcaacaggc cggcaggcat ttgtaacaga agcgcaaaca acagcagcat cagctcagtc
       61 gtcatacgaa agaagtgttg aacagctgga aaaaatattt accgatgcag tggcttatgc
      121 agcagttgaa aaacctgaaa agacaaacct caggcttgaa gcaatgagag gactctttaa
      181 cggttcgaag acactcttta tcaacgccca ggatgcaaaa ggaattattg cggcagtttc
      241 gtttgctaaa cgctatggag tgaaaaagat agttcttgca aatgcaaccg aatcagcatg
      301 ggaagtgaaa gatttcctga aagagaacaa tatacccgtt attgtggcta accctcttag
      361 cctcccatta tacgagcata gcgacgtgag aatgcccttc aaattggctg caatgtttat
      421 gaaggaaggc atacttacag gccttacata ttcctcaatg gcttatggca acctgccttt
      481 tgcagctgga caaacagttg cttatggcct tacaaaagaa gaagcattgc aaactgtcac
      541 actcaataat gcgaaaatac ttggtataga tgatgttaca ggctcccttg aacctggtaa
      601 ggatgccaac atagtggtgt cgacaggcga ccttctcgac atgaaaacca acaacgtgga
      661 acttgcattc atcacaggaa gaaatataag ccttgataat aaacataaac aacttaccag
      721 gagatttaaa gagaaataca ataatgtgaa ttccggtaac tgaaattgtt gctcaatatt
      781 tgagtttacc agaaataaca ttctgaaggg cacatttata catactcatt gattttttat
      841 ctgcttttat tgtgtcccat agcatgttat tggacaggca aaattaccgg gccttcttta
      901 tgataattat tcagtggtga aaaccaataa tatgacctgc cctggatttt agcaattttt
      961 catccaatta tcactcaggg taagcagagt tttgtatttt tatgctatga aaatcattat
     1021 tgcaggtact gcttatccat tcaggggcgg actggcagca tacaacgaac gccttgcacg
     1081 tcagtttcag tctgaaggtc atgacgtttc cataataact tttacgacac aatacccttc
     1141 atttcttttc ccgggtaaat cacagttttc atcagctccg gctccttctg accttaaaat
     1201 agaaagaagg atgagttctg tcaatccgtt tacatggata attaatggct tgaaaatcaa
     1261 gaagaccagg cctgatatcc tcattttcaa atactggctg ccatttatgg caccatgttt
     1321 cggaaccata gcacgcatgg caaagtcgaa ccggcataca aaaatcattg caatcttcga
     1381 taatgttata ccacacgaaa aacgtccggg cgacacattc ttaactaatt atttcgttaa
     1441 atctgttgac ggtgctattg tgatgtcaga aacggtaaaa accgacctgc tgaagtttcg
     1501 gagcaatata ccggtgtatc tgacaccaca tccgctattc gataacttcg gccagaaaat
     1561 agaaaaaaag gaagcactta cccggctcgg attggatccc tctttcaatt atatcctatt
     1621 ttttggtttt gtaagacctt acaagggtct ggacatcctt ataaaagctt tcaaatgggt
     1681 gcgagaagaa tacactgaga caaaactgat agttgctggc gagttttatg aagacgaaaa
     1741 gccttataga aaactcattg aagaagaaaa actgcaaaat gatatttttc tgttcaacag
     1801 gtttataaat gacgatgaag tggcactgtt cttttctgcc tgcgatctgg tagttcaacc
     1861 ttacaaaagt gccacacaga gcggagtaac ccagatagca taccactttg aaatacccat
     1921 gatagtaact aaggtgggag ggttgaaaga aattgttccc ggtggaaaat gcggatatgt
     1981 tgttgaaccc gacccgattg aaattgccga tgctataacc cgtttctatt ctgagagaaa
     2041 ttctattgac tttaccagtt acatcagaga ggaaaagaaa aaatattcat gggataaaat
     2101 gacagcagta attttagata tttacagaaa aatttctgaa gatgatagta agaagtaaag
     2161 cccccctcag gttaggcctt gccggaggtg gaactgatgt atcaccatat tccgacatat
     2221 ttggaggagc tatccttaat gcatcaataa atctttatgc atatgcctca atagaaccgg
     2281 gggataatgg agaaattact ctcagacttg aagataagaa tcttgaactc aggtaccctc
     2341 tgactgagcg gctcgaacct gatggtaata tggacctgat aaagggtgta tacaacagga
     2401 ttatcaggga attcaggctt tcaccacaac ctttcaggct ttctgcttat gttgatgcac
     2461 ctccggggtc cggtttgggt tcctcctcca cactggttgt agcagtactt ggagcatttg
     2521 ccgaatggtt ccggcttccc ctcggagaat atgaccttgc ctggctcgcc tgcgatatcg
     2581 aacgtaacga cctcggaatg gcaggaggga aacaggacca gtatgctgca actttcggcg
     2641 gagtaaactt tatggaattc tataaaaacg acaaagttat tgtaaacccc ctccgcatta
     2701 aagaacagat cctgaacgaa ctctccaaca acatagtact ctattatacc gaaacaagca
     2761 gaatatcctc aagcatcatc gaaaaccagc aacggaatgt taaagaaggc gtttccgaat
     2821 cggtggaagc aatgcacatg cttaaggagc tggctattag tatgaaagaa gcattgctga
     2881 agggggaact tgactctctg ggagaattac tcgacaactc atggaattat aagaagaaga
     2941 tggcgggcgg aataacaaac ccccacatcg acacaattta tgatacagcc ctcaaagcag
     3001 gagctaccgg aggtaaaata agcggggcag gagggggagg atatatgttc ttctattgcc
     3061 cccgtaatac aagatataac gttatccggg ctcttaaaca attcggtgga catgagcaga
     3121 agtacgaatt taccaagcgc ggactcgaaa cctggactat ctgaataatg atcaatgatc
     3181 aaagaagccg taatacttgc aggaggactc ggaacacggc tgcgtggagt ggttgacgat
     3241 ttaccaaaac ccatggcccc agtaagagga caaccatttc ttacatggct ttttgattta
     3301 attgaaaaac aaggcgttga aaaagttgtc cttgccactg gatataaaca cgaaaaaata
     3361 tcatccttct ttgactttag gtataaaaac ctcaatattg aatactctgt tgaagccgaa
     3421 ccactcggaa caggaggggc cgtgttaatg gcattacaaa aaattaaagg agaaaatttt
     3481 tttcttctta acggcgacac ttattttgaa gttaattttc atgattttga gttttttttt
     3541 aattccaccg gctcagttat gtcagttgcc ctgcatccgg taaaaaatgc cgctcgctac
     3601 ggtacagtaa ttcttcagga aaacagaata gtggcattcg gcgaaaaaac tgcaaataga
     3661 gggggcttaa taaacggagg catttatgtt gtgaataaaa aatggtttga agagaacgcc
     3721 cctggagaaa aattttcact cgaaaaagac gtgctcgaga aaaaaatttc tgacaatctg
     3781 attaccggat atgtttcaga tgcctatttt atcgacatag gtattccgga agattattac
     3841 agggcaggtg aggaacttcc gcctgttggc tgatattcag gcatttattt tgtctttcag
     3901 caaatattta tttctttcgg gtgaatttct gttcacaagc tcaccaataa acccggtcaa
     3961 aaacagcagc gtacctataa ccatcactgt aagtgcaata tagaaccagg cactgtcggt
     4021 taccagagga gctctcatat tgtgcgaaac ataccagagt ttcctgcctc cgaggtatcc
     4081 tgccattaaa aagcctgcca gaaacatcag cgtgccaagt aaaccgaaaa gatgcatcgg
     4141 actcttgccg aacctggtga taaagcctat agtcaacagg tcaaggtacc ctttgatgaa
     4201 cctgtcgaga ccatattttg ttgttccata ttttcttggc cggtgctgca caaccttctc
     4261 accgatcttc ttaaatcctg cttctttcac aagaatagga atatagcgat gcatctcccc
     4321 aaagacctca atacttttta taacctcatt cctgtaagcc tttaacccac agttgaaatc
     4381 atgcagtctg atacccgacc atacttttgc cgtaaagttg tagaatctgc ttgtaactct
     4441 tttgacaaac ggatcatatc ttttcttctt ccatcccgaa acaaggtcat aaccttcttc
     4501 ctttaccatt cggtataact caggaatttc gtccggacta tcctgcaggt ctgagtccat
     4561 ggtaatcaca acatcgcctg aagcaagccc gaacccggtg tgcagagccg ccgccttccc
     4621 ataattcctt ctgaaactta cacctttaat attattgttc gacctgcaca gatcgccgat
     4681 aactttccat gatgaatcac tgcttccatc gtcaatgaaa attatctcat aggtaaaacc
     4741 tgaattgcgg caaactctta caatccattc tgccagctca ggcaacgatt cctcttcgtt
     4801 atatacaggt atgacaacag acagatccat ctgtttaaat taattttctg ggatgtcaat
     4861 caggggatta ccttctttcc ttgtaaaaat gctaaccagc agcgacataa ttgtaccaag
     4921 gagcatgttg ttgattatgc ttgccggggc gataaactct ggaaccaata tctttttctg
     4981 tatgttcatg ctggtgtcaa tcatttcctg gctcatacca cggcgggcaa gcatctcttc
     5041 agccatttca agctgtttgt caattagccc ggggtcgatg aatttgtaaa gaatatacgt
     5101 aaatagggca gcgatgacag aaaagtacag aaaaatcact acaccagcac caacactctg
     5161 accgtacttt atgtacccgt ggagataact gtcgcggtat gattttacca ttaaatagag
     5221 tattatcact tcggctgcga gaaaaatata accctgagtc ctgttaagtg aaaggtccag
     5281 aaaatacatt atcagagtat atacaatgta tatcagcccg aatattaaac cattagtaag
     5341 gtttgctttc cagacggaaa ttttttcttc catgaaataa attattaggt ttgacttaca
     5401 aacaaatata atttaaaaaa ccaattttaa catcatttgc cctgtgcata aattttatta
     5461 attttgcaca gggtaagtct tatacgacca gctcctgctg aactccccca ggaccggaag
     5521 gtagcaaggg taggcggttg tagcggtgcg atataagtaa cttacccttt ttaagtttat
     5581 tccatggtct ttgtgttttg atagttttaa accttatatc cgtctgaaaa aggtgaattt
     5641 ttacttctca ttttgtatat ttgaaaaaag tagtcgctat gttgttaaaa attcatcctg
     5701 agaatcccgg taccatacag atccgcaaag tggtggaagt tcttgaaaag ggcggagtta
     5761 ttatctgccc caccgatact gtttatgcca tgggatgcga tataaaagca actcatgcaa
     5821 ttgagaaaat tgcaaggttt aagggaactg accccaaaaa ccctgacatg tcattaattt
     5881 ttcatgacat gagtcaactg agcgagtaca ccataataaa ggataacaat atcttcaagc
     5941 ttttgaaaag aaacctcccg ggcccattta catttattgt caaggcaaat aaccagattc
     6001 ccaaaatgtt cagaaacaga aagaaaactg taggaatcag gataccgaaa aataacatta
     6061 tccttgaaat tgtgagggaa ctgggcagac cacttataac cacatcaatt catgaccgca
     6121 atgaaataat tgaatataca actgaccctg aactgattta tgaaagatac agggacatgg
     6181 ttgatcttgt gataaacggt ggttacggaa gcaatgaacc ttcaactgtt gtggattgca
     6241 caggtgaaga accggtaata ataaggcagg gtctgggaac tcttgaattt tagaaggtca
     6301 attatttcct gaaaaagctt cttgcaaaaa gcaccattac tgcaagcatt tcaagtcggc
     6361 ctagtatcat cagaaacgac ataaaccact tgcctgctac aggaagggag gagtaggtgg
     6421 ttaatggccc aaattctcca attgctggcc ctatattggc cagaattgat gctgcagtac
     6481 tgaaagaagt tattatgtca taattcatca tggaaaaaat gaatgcactg ctgcaaatta
     6541 ttataaaata gattgttaca aatacaagta tattgtaaac tatttcctgt ggaataattt
     6601 tacggttaat tcttacaggc aggtaggcat cgggatgtat gagccttttg atttccattc
     6661 tggtatttat acccagtgtc atcagtcttg caatttttat tcctccgctt gcagaaccca
     6721 ctgtaccgcc tgtaaacatc agaataaaca acagcagcag tatgaaatta ccccattggg
     6781 tataatcgtt tgtgtaaaaa cctgttgtgg ttattattga tatcacatgg aaagagccgt
     6841 ttatgaccga ttcggagaaa gggtgtttct gaaccatcaa aagccccgat actaccagac
     6901 agaatgccag agataggaca agataataaa tgaactcgcg gttctcttta atcttctgta
     6961 aatttctttt atacagataa taaatgatag ccatattggt tccgcccaga aacataaaaa
     7021 tagtgataac aatttttgca tacggtgctg taagtagtgt cagactgtag ttgtgcggag
     7081 aaaaaccacc ggtagagagt gtgcttaacg agatgcaggc tgcgtcgaac aggttcattc
     7141 ctccggctgc cagaaaaaga gcctcaagaa tggtaagaat aatatataat gaaatcagcc
     7201 ttttagcagt gtcgacccac cgggggtaga ttttatcctg aggctgacct gaaaaatctg
     7261 tggttgaaag ctgaatgata ttgtccttga atacaggcag gacatacagc gacagaaaaa
     7321 ttattcccag tccaccaatc cactgggtaa ggcttcgcca taaaactatt cccctgggta
     7381 gtgaatcgat actgttcaga atggtagctc ctgtagtcgt aaaacctgaa gctgattcga
     7441 aaaatgcatc agttatattc tttattgttc cgctgagcag gtaggggagc atgccggtga
     7501 tgatgtaaat cagaaatgtc cctgttatta taagaaagcc ttcacgattg ctgtagagtt
     7561 gttcatcccc ttttactggt gtataaacga aaacaccagc tgtaattgta attaatcctg
     7621 aaaaaagaag cgatgaggct gctgggtctt taagaaaata agatatgcct gcagcggcaa
     7681 gcatcattat tccctcgaaa attagcatta agccatatat ccgtgctatg atcctgaaat
     7741 tgatcataca cgatcagata aaaaatttag agaccttttc aaacgccgaa ggaagcgtga
     7801 atactacaac cctatcgttg gctttaataa tagtatcacc tgtagctatg aatggtactc
     7861 catcccttgt tcctcctcca acaattgctt cgtcggggaa atctatactg cggagtgtac
     7921 cctttgttat tcttgcattt accggaacga tgaattcaac aacttctcct tctgcacctg
     7981 caatgtattt aacctgtgtt acattgggat ttgttgtata tctgaaaatc ctgctggcag
     8041 ccgctatctt cttgttaatt atggtgtcta ttcctatatt ctcagccaga tggatatatt
     8101 cagtgttttc aacttcggcg atggttttct ttacccccat cttctttgca agaagacacg
     8161 aaagaatatt tgtttcggag tttcctgtta ctgccacaaa gcagtccatg ttgcctattc
     8221 cctcccggct cagaaggtct gcatcgcggc aatcgccatt gataatcaga gtttgctcaa
     8281 gaagttctgc aagtgcctgg cacttttcat agtccgaatc aattagtttg acagcactgg
     8341 ttttctgcat gtgcaatgct acatgcttcc cgattctgct tcctcccact atcattatgc
     8401 tcttagcctc aaaactttct ttacccgatg ttttcatcat ctcgtctatg ccttcacgtg
     8461 tcgaaatcag atacacaaca tcgccaggtt gaaacacttc ttcagcactc ggaatgatgg
     8521 ttttttcgtt tctttttatt gcaattgccc tgtactgatc tgttttgtaa agtttggcaa
     8581 tctctttgag acttttattc agtatcggag ccttctcatc cagtttctga acatacataa
     8641 caagctgccc accagagaat tccataaagt cagtcgtccc ggtttcatga agaagcatca
     8701 gtacctctct ggcagctatc aactcgggat atattaacga atcaataccc aatgacttga
     8761 aaaactcttc gttctcccgg ttgagatact ccagattgtc aatccttgca atagttttta
     8821 ttgctcccag cctcctggca agaatagaag ccgtaatatt ggtgtcttca gaatgagtta
     8881 cagcaatgaa cagatcagtc tttttcttca ttgcattctg aaggaggctg acagaagtag
     8941 ctgatccatg caaagttaat acatcaagtg ttgaatcaat gggcttcagc ctttcctcat
     9001 caggatcaat aatgaggatt tcatgatttt cattcgacaa catcttggcc agatgggttc
     9061 caacctcacc ggcacctgca attataattc tcataaaaag aattattgaa aaatcaccac
     9121 aaaataagat ataaaatatt taatactcaa gtttgtttga atatttattc tcttttcagc
     9181 ttcattctga agcacccctg gctttttata aaccataagc cgttgaccct gttattcctg
     9241 accttgtttt caaacaccct gccaggatat accgaagtta atataatatt atctggtttt
     9301 gcactttcag ataaaaatag aggtcttaag cctgtgtaaa tcactatatc agattttttt
     9361 acagcatttt ctgccgggta actatcagat atcaagatat ttttacctcc ggctcttatt
     9421 aagaagggca ttttacctct tacatcaata gttttcacat gtaaacccgt gattgcactg
     9481 tgtcgtaatg cttcacctgg cactttttcc cccatgtaaa aaagattcag tgtattaccg
     9541 ttctttatcc ctgcaacagg aactgactga gtattataaa ctatcagttc atttttgaaa
     9601 cctgaaatta ccttctttgt tgtaactgta acaattagag ccagaaaagc tgcaagtggt
     9661 aatacaacac ttttcttttt ttctgaaagc aagtaattaa gaaaaattgc tagtgctatg
     9721 ataagaagaa tgctttcggg taccgttatt ccagtgtttt caagtgatga gaaaggaagc
     9781 attgaagtct tttcagtaag atatcctgca agaaatgagg ttctgtccat cactgttaca
     9841 aaaatcgatg cgacaaatcc tacaggagct gtaataacag ccagaaaacc cgatattatt
     9901 attactgcag ccagaggaac aattaaaata ttcgataaaa gaaaaataag cgaaaaacgg
     9961 ttgaacattg ccagtgtcag tggtagtgtt cctgcctggg caacaaatga caccgaaata
    10021 agttgccata tcctgtcgga catgaaatta ttgaaagtca atgtattata aaagtctctg
    10081 tagaaaagta ttataaaaaa tactgcaaca tacgaaagct gaaatgccgg atcgaaaata
    10141 accagaggat ttattactga aagaataaaa gctgatgcca gcagggagtt taacggattg
    10201 atttttctgt taagaagctg accggtatga aggaatgaaa acatcaggga agccctcata
    10261 actgaaggag ataaacctgt tgtaaaggca aaaaaccata atgtggctat tgtaaaaaat
    10321 accctgagaa ttttaagttt tccttttaaa aagaataata tattgaaaat gaacagactg
    10381 agtacacctg cgtgaagccc tgaaactgcc ataatatgca ttacaccggc tctggaaaaa
    10441 tgttcttttt catcatcgcc gagcatactt ttctctccaa gggttattgc agcggccagt
    10501 gcaagccttt taccccgggc acctttttcc tggtacatgt taatgatctg ctcacggaaa
    10561 atgagcgctt tttctctgag tgtccttcgt gatggtgatc cataatatat aatatcatct
    10621 ttctgaatga atccgtaata cctgaatccc tttgattcca tataaaacct gtaatcaaat
    10681 tcatacggat ttcctttgtt tcttatttcc agaggcgtgg tcctgaacat aataatatct
    10741 ccgggcatca gttttgtcag ctggctattt ttgctatggt aaagaattat tgaaccttct
    10801 atcctctttt tcacactatc gctccagata tacttcagct ttgattttat caggaacgaa
    10861 ttttcttttt cttcgggaaa atcaagtaca actgaacaaa acaattgttc tttctgttcg
    10921 agtgtggaaa ggcttcttct ttcatttaca gcaagaataa cgcccgaaag aaacaatgca
    10981 gcagtgagga ttgctccaaa cgccgtatta ttgtatgatt taattgtgaa gtagctgatt
    11041 aacagtggta tgattaaaaa gacagaaaga agaagcatta ttttaatgcc gattccttct
    11101 gcgcaaaaac tggctgcaat gcccaggcag taagggataa atatccgcaa aaagggtatc
    11161 tccttgaaaa gcacgatatg cagctaatga gactgcataa agataataaa aaactatttc
    11221 tttcgtggtg tatatacgat agttttgtag tcggcttcga attttccttt gctgatatcg
    11281 ttaagtttac cctttatcag tctttttctc agtggattaa tcttttccac aaaaagtatt
    11341 ccgtcgagat gatcatattc atgcaggaag actcttgcct tatatccttc aaaagtttca
    11401 tcgtggaatt cccagttttc gtcgtaatac tgaactctga tgtgtgattc tctctttact
    11461 tcttcacgaa tattcggtat gctcaggcac ccctcgttca ttggtacaag ttccccgtct
    11521 ttctcaatta ttttcggatt tataaatact ctcttgaaat ttgcaaattc aggttcatct
    11581 tctgccagag gggcaccatc aataacaaat accctgattg attttccaat ctggggagct
    11641 gcaagcccca ctccctcagc agcgtacatt gtttcaaaca tgtcatcgat tagctgcttc
    11701 agttcagggt aattcctctc tacttccgcg gcaactttct ttaaaacggg atgtccgtaa
    11761 acaacaaccg gatatatcat tttgttattt tttactgtta agaaaagacc ttttctccag
    11821 aaacgattga aggataagtg aagcactaat tttatcgacc agtgatttat ccatcctcct
    11881 ttttttcttc acaccacctt caatcagtgt ctgctgacct atcaccgaag tgaatctctc
    11941 atcagccaga taaattgttt tatccgggta gagctttctc attcttctga tgaacggttc
    12001 gatatattta acagcttcac tgggctcatt gttcatttgt aacgggtaac cgataacaat
    12061 ttcatcaaca gtttcttttt tgaaatagtc agagagaaag ttctcaactt cgtttaccgg
    12121 aactgtttcg agtggtgaag caattatccg cataggatct gtaactgcta gacccacgcg
    12181 tttctttcca taatctatag caagtattcg tcccattgaa aatcaagtgc aaagataaaa
    12241 ataaaaacaa aagggggttg ccccccttgt tgtctatttt agcattaaat agtaaaacta
    12301 ctgttgtgga tattgcatct ctgcaagacg tttataggtt ttgtatctcc attttgcatt
    12361 ttcctcggct gctttgaaca gagcctcggc ttctttcggg ttcgtcttaa gcagggcagt
    12421 ataacgtact tccgaatgca ggaattcctg gaagagtgac cagtcgggtt ccttcgaatc
    12481 gaggatgaaa ggattcttcc cttcagcttc aagcagagga ttgtatctgt aaagatgcca
    12541 gtaaccggcc ttaacagctc tctcttcctg ttcctgcgac ttgcccattc cgtactttag
    12601 tccatggttt atacaaggag catatgcaat aatcagcgac ggacccggat atgcctcagc
    12661 ttccttaaca gctttaagca gctgtgcgtt gtttgcaccc atggcaacct gtgcaacata
    12721 aacataacca taacttattg ccatcagacc gaggtctttt ttcctgatct ttttacctgc
    12781 tgctgcaaat tttgcaactg ctgctgcagg ggttgatttg gatgcctgtc cgcctgtgtt
    12841 ggaataaacc tctgtatcga gtacaagtaa gtttacatcc tcaccctggg cgagcacatg
    12901 gtcgagtccg ccaaaaccta tatcataagc ccagccatca ccaccgatta tccagtgaga
    12961 tttctttaca aggtattgtt tgagactcag tatctctctg gcaacctcgg aaccgtcctt
    13021 ttcacacgct tccacaacct tcgccgatgc aacctttgaa gcttctgcat tgtttctgga
    13081 ggcgagccat tcttcgaatg ccgcttttgt ttcggaatta accctgccat tgcttagggc
    13141 t
//
LOCUS       DAOSNB010000032        12807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4891_length_12807_cov_4.813206, whole genome shotgun sequence.
ACCESSION   DAOSNB010000032 DAOSNB010000000
VERSION     DAOSNB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4891_length_12807_cov_4.813206"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..581
                     /locus_tag="PLG42_02580"
     CDS             153..581
                     /locus_tag="PLG42_02580"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOU30128.1"
                     /translation="MKILILCTGNSCRSQMAHGFLQSFDSRLEVHSAGTEPGNEVNSR
                     AVKVMAEVGIDISRHYPKHVEKYINDEWDYVITVCDSANETCPIFTGKVKQRLHMGYE
                     DPSFKVGPEDYIMNEFRRVRDQIKEDFYRLYIEKIKPELK"
     gene            578..1018
                     /locus_tag="PLG42_02585"
     CDS             578..1018
                     /locus_tag="PLG42_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOU30129.1"
                     /translation="MTKSEKAVDYFRKNYNCAQSVLTAFGPDYGLSEDFCMKVACAFG
                     GGMGRQQYTCGAVTGALMVLGLKYGKGLNNPEEKKRHTYSLTKKFISEFIRINGSSNC
                     RELLEGLDMNNEDDLKVIKEKNLFETRCEKFIADAVDILEKILK"
     gene            1028..1828
                     /gene="arsM"
                     /locus_tag="PLG42_02590"
     CDS             1028..1828
                     /gene="arsM"
                     /locus_tag="PLG42_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOU30130.1"
                     /translation="MKPDEIKNIVRDKYENIAGFEGENKASCCSSACCCTGEDITAFN
                     EKYGERPGYISEADLGLGCGIPTDFAGIKQEDHVLDLGSGAGNDCFVARALVGEKGRV
                     TGIDFTRKMVEKARENCQKLGYNNVEFVEGDIEAMPFEDNSFDVVLSNCVLNLVPDKA
                     KAFSEIYRVLKPGGHFCISDVVLKGQLSEKTRHNAELYAGCISGACSIEEYIGIISKT
                     GFRNITVHKQRKISIPPEIEFEMESQSLNDEIEGDEKGIFSITVSAIK"
     gene            1878..2921
                     /gene="arsB"
                     /locus_tag="PLG42_02595"
     CDS             1878..2921
                     /gene="arsB"
                     /locus_tag="PLG42_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008023067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOU30131.1"
                     /translation="MEAIKRLSFFDRYLTLWIFLVMFAGVGAGYFFPRIADFWNSLSS
                     GTTNIPIAIGLILMMYPPLAKVRYEKLPLVFKNFRLLGLSLSMNWIIGPIVMFLLAIL
                     FLHNHPGLMVGVILVGLARCIAMVLVWNDLAKGDTELAAGLVAFNALFQVFFYSVYAY
                     VFITLLPPLFGLKGEIVEVSISEIAVTVLIYLGIPFFAGLITNIILRRTAGDEWYYRK
                     FIPKASWLTPIALLFTIFVMFSLKGEKIIELPLDVVRVAIPYTIYFAVMFFSTFFIAK
                     LMGSGYEKSTTMAFTAGSNDFELAIAVAVAVFGIASPAAFATVIGPLVEVPVLIMLVN
                     VALYFRKKYFAEA"
     gene            3087..3992
                     /locus_tag="PLG42_02600"
     CDS             3087..3992
                     /locus_tag="PLG42_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU30132.1"
                     /translation="MRNKFCLKSAVNGIVTLILFFALSGCKGKSDSFSFVFMSDIHLT
                     YERNAIPGFKKAIEEVNKLKPDFVITGGDLIMDALSQRYTMADSLYNLYSEMVKEIKA
                     PVYNTMGNHELYGIYSRSGADPSNPEYGEKMFEKRLGKSYYSFDYKGWKFLVLNSVED
                     TGKDRYIGFIDSTQINWIKEELQKTDTLTPVVLVTHMPFITVNSQMYRYSTASNDSGL
                     VVVNSREVLDLFSHYNLKLVLQGHLHMVEDIYIDGIRFITGGAVSANWWRGPYRGDEE
                     GFILVNVKGDDFTWKFIDYGWEVQK"
     gene            4026..4772
                     /locus_tag="PLG42_02605"
     CDS             4026..4772
                     /locus_tag="PLG42_02605"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOU30133.1"
                     /translation="MLKSVMNFRDAGDSDPAGGTGLRKGLIYRSGSLDRIKREDIRRL
                     QQLGIKAIVDLRAPAEYSKRKVVIPGINVFSMPLDFEYVTRERLKPLIMSGNQEEAVQ
                     KVITELYREILDAAIPIYREIVEMILDGGHTPMLIHCRAGKDRTGIICALLQMAAGSS
                     EDSIIRNFMKSNDVIVPYYRKRLWIRKILSLGFFPADAILDAITLRGENIREVIKRVR
                     VHYGGIAGYLSVSGFDMSKFMLLKGKLNNI"
     gene            4777..5190
                     /locus_tag="PLG42_02610"
     CDS             4777..5190
                     /locus_tag="PLG42_02610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOU30134.1"
                     /translation="MMVQANDEKVREASEIKKRIYSFRNAFRGIWTVFRFEKNFRIQF
                     IIFLLVIAAGIVLDISVVGWIAVIIVSGLVFASECFNTAVEYLGDAITEGEDINIKKA
                     KDVAAAGVLISAIISVITGFIVFLPAVLRSLGLRV"
     gene            5526..5972
                     /locus_tag="PLG42_02615"
     CDS             5526..5972
                     /locus_tag="PLG42_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30135.1"
                     /translation="MTLKFILLAVFGIAMAHLEGVVVVYLRKAIGILDSDSNKEAVNQ
                     INPWYLKVEMTREAATIIMLAVIAYLTGTSWVEKGIFFLWTFAFWDLFYYLSLFILIK
                     WPPSLNTIDVLFLIPKPWIAPVWFPIGVSSLTIIVIAILYLFRIIP"
     gene            complement(5994..6173)
                     /locus_tag="PLG42_02620"
     CDS             complement(5994..6173)
                     /locus_tag="PLG42_02620"
                     /inference="COORDINATES: protein motif:HMM:NF028184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HOU30136.1"
                     /translation="MGLDPEKTYRIEEINIMPGLRRQGTDSGRSYTGDYLMKTGLSIG
                     SATPLTSVVYEITSQ"
     gene            6437..9106
                     /locus_tag="PLG42_02625"
     CDS             6437..9106
                     /locus_tag="PLG42_02625"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU30137.1"
                     /translation="MKKIIAHLIIILFTATLLPFQKLYGAFQQYEAAPHDTIKKLKTY
                     SGQPVYITSRLVTSRPVIDGKLDDECWKHGNWAGDYTQFIPNEGAKPTYPTEFNIQYD
                     DKYLYIAFRAYDGEPGKIQKFAGARDETVGDMVGVTFDSYRDYRTGFEFTITAWGQKV
                     DLVLFNPMNWDFNWNAVWKGKTGLEDSAWVAELEVPLSQLRYSNQYEQVWGMHTWRWI
                     NRLQEESNWERQSKTGPGMLYNFGELRGINGLKKSRRLEIMPFMLGKVETMKTEPGNP
                     FTANGKIWGGNAGLDAKIGVSSNFTIDLTVNPDFGQVESDPSVMNLTAFETFYEEKRP
                     FFLEGLTIFDYKFDDKTLFYSRRIGHSPSLLIKGGDDLFVKMPDMTTILSALKFSGTT
                     SKGLSLGFIQALTSDEYARVSDGGGNRSRKMVEPLTNYMVARVQKGYNEGATVLGAMM
                     TSTSRFIKDPELEFLARNAITGGLDLIHRWHDKEFYIDARLLGSFVEGSQRAIRELQE
                     SSARYYQRPGADYLDYDTLRTSLSGFGGKFRIGKGAKGFWRYSTGVSWLSPGLELNDL
                     GYMRTSDEINQENEISYFVNQPVSVFRTYTITLEQFNSWNFGGIYLGSGSHLSFESEF
                     KNNLALSTNFIIHSGSSDPRLLRGGSDFKVPYSVMSFGKIRTNPAKKLIISLNYDYSY
                     SGHDSRTNYEITPGISLRPFNALRFEMTASFGKNKDELQYVTTLNTNEGKRYILGTID
                     QQTLNLTFRVDLNITPEFSVQYYGSPFVSKGRYSEFKKVTNPLARNYSDRFSVFENPV
                     LSGNMYFLDDNGNFIPDYSIENPDFNFHQFRSNLVAKWEYRLGSYIYLVWSSDRTGFM
                     DQPASSIGESFRQLWKVFPKNIFLIKLNYWFSL"
     gene            9167..10492
                     /locus_tag="PLG42_02630"
     CDS             9167..10492
                     /locus_tag="PLG42_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU30138.1"
                     /translation="MKRLDIAARINLIFRSLRYRNYRLFFGGQSISLIGTWMQRIALP
                     WMVYRMTGSAFMLGLVGFVGQIPTFILAPVAGVVTDRFHRYRVLIITQIVSMAQASVL
                     AYLTLSGDIKLWHIIVLSIILGCVTAFDVPSRHSFVVDMVEKKEDLGNAIALNSMMFN
                     GARLIGPSIAGVILAVTSEGICFLLNAISFIFVIASLLMMKVDGKKKSATKSGLFSDL
                     KEGFLYAFGFAPIKHIILLLALVSLMGTSYQVLMPVYAKEVLKGGSEIFGFLMGAAGL
                     GAFLAALYLASRETVITLGRIIPLATGIAGAALTIISLEDLFFLTFFLMVFVGLGLML
                     HTVSSNTILQTITDDDKRGRIMSFYTMSIMGIAPFGSLIAGAMAKALGTRLTLLIGGI
                     SCLAGAYFFYRKLPLLKKIARPKYEKMGMIPEIVTGIQIASEPDLKTEE"
     gene            10554..11696
                     /locus_tag="PLG42_02635"
     CDS             10554..11696
                     /locus_tag="PLG42_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOU30139.1"
                     /translation="MSEELDDFQIFVKPIGAKCNLHCSYCYYLNKENLYASESYKTLP
                     EDILEEYIIQHFEASSGTDVFFSWHGGEPLLAGIEFYRKAIELQNKHKPESFTVYNGI
                     QTNGILLNDKWCRFLAGNDFYVGISLDGPAEFHNAFRVYRDGRSSFDKALEGYRLLQQ
                     YNIRNEILCVVSSVNVRKPLEIYRFFKSLGVRFLTFLPLVIRDISKPGQVSDISVPAE
                     EFGQFLCKIFDEWVLEDIGRIKIQIVEEAIRPAFGQEHSLCIFKKICGRVPVVELNGD
                     FYSCDHFVDHEHLVGNIMKQSVSDLLNSDKQKNFGMAKLITLPEYCRQCDVREMCNGE
                     CPKNRFVTAPGGEPGLNYLCAGYRMFFRHVKPFAEAVGAEWRRRNR"
     gene            complement(11700..12551)
                     /locus_tag="PLG42_02640"
     CDS             complement(11700..12551)
                     /locus_tag="PLG42_02640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOU30140.1"
                     /translation="MEKEIKSLYEEYKRGETSRREFLRKLILYTGSVTAALAILPELG
                     DEHTALAEYQDFPGLVTEFIKYPGFNCDMRAYLARPVKPRKNPAVIVIHENRGLVPHI
                     QDVTRRMAKEGFLALAPDALSPVGGTPDDVSNVGELFKKLNNDDTVKNFVAAVKYLET
                     HPNSNGKVGCTGFCWGGAMTNQVAVNAPTLKAAVPYYGRQPAVEDVPKIKAAIMAHYA
                     ENDPGINAGIQNFEQALKKANIEHYIYMYPGTSHAFNNDTGPRYNEEAAKLAWQRTVE
                     FFKKKLK"
BASE COUNT         3881 a         2147 c         2798 g         3981 t
ORIGIN      
        1 atgtaaatcc tcacggggtg gcttaaggcc ttgaatattt tagccagctc gaggatttca
       61 gtactgaatt caggtcttgg tgacatgata ttttatttta atatttcgcg aaattacgaa
      121 atatttttat atttgcaaaa aacttgaaaa aagtgaagat acttatatta tgtacaggca
      181 acagctgtcg cagccagatg gctcatggtt ttttgcagtc gttcgacagc aggcttgaag
      241 tacactcggc gggaacagaa ccgggcaatg aggtaaacag cagggcggta aaagttatgg
      301 cagaagttgg tattgatatt agccggcatt atccaaaaca tgtagaaaag tatattaacg
      361 atgaatggga ttatgtgatc actgtgtgcg acagtgcaaa tgaaacttgc ccaatcttta
      421 cgggtaaagt aaagcaacga ctgcatatgg gttatgaaga tccttcattc aaagtcggtc
      481 cggaagatta tatcatgaac gaattccgta gagtaaggga tcagataaaa gaagattttt
      541 acagattata tattgaaaaa ataaaaccgg agctcaaatg acaaaatcag aaaaagcagt
      601 tgattatttc aggaagaatt ataactgtgc acagtcagtg ttaacagctt ttggaccgga
      661 ttacggatta tcggaagatt tttgtatgaa agttgcttgt gctttcggcg ggggaatggg
      721 acgacagcaa tacacatgcg gagcagttac cggtgcactc atggtgcttg gacttaaata
      781 cggaaaaggg ttgaataatc ctgaagaaaa aaagagacat acttattcac tgacaaaaaa
      841 gtttatcagt gaatttataa ggattaacgg ttcgtcgaat tgccgtgagt tactcgaagg
      901 attggatatg aataatgaag atgatttgaa agtcataaaa gaaaaaaatc tttttgaaac
      961 gagatgtgaa aaatttattg ctgatgcggt agatattctt gaaaagattc taaaataaat
     1021 cattaacatg aaaccagatg aaataaaaaa tattgtaagg gataaatatg aaaatattgc
     1081 aggttttgag ggtgaaaata aagcatcatg ttgcagcagt gcatgttgtt gtacaggtga
     1141 agatattact gcattcaacg aaaaatatgg agaacgtccc ggatatattt ctgaagctga
     1201 tctgggtctg gggtgcggca ttccaacaga ttttgcaggg ataaaacagg aggatcatgt
     1261 tcttgacctg ggtagtggcg ccgggaatga ctgctttgtc gccagggcac ttgtagggga
     1321 aaaaggtcgt gttacaggaa tagattttac ccggaaaatg gttgaaaaag cgcgtgaaaa
     1381 ctgtcagaag ctgggttata acaatgttga atttgtggaa ggagatattg aggcaatgcc
     1441 ttttgaggat aattcatttg atgttgttct gtccaattgt gttcttaatc ttgttcccga
     1501 caaggcaaaa gctttcagcg aaatatacag ggtcttgaaa ccaggaggac atttctgcat
     1561 ctcagacgtt gttctgaaag gacaactctc tgaaaaaacc aggcataatg ctgaattata
     1621 tgctggatgt atttctggag catgcagtat tgaagaatat attggtatta taagcaaaac
     1681 aggtttcagg aatattactg ttcataaaca gagaaaaatc agcatacctc ctgaaattga
     1741 atttgaaatg gaatcacaat cattaaatga tgaaattgaa ggtgacgaga aaggtatttt
     1801 cagcataaca gtttcggcaa taaaataaaa aagttcaaat tgattccaaa tcttcaaatt
     1861 tttatattat taatcctatg gaagcaatta aaagactatc attttttgac cgctatctga
     1921 cattatggat ttttcttgtg atgtttgccg gcgtaggtgc aggatatttt ttcccgcgta
     1981 tagctgattt ctggaattca ttaagctccg gtactacgaa tataccaatt gccatcgggc
     2041 ttatattaat gatgtatcct cctcttgcaa aggtcaggta tgaaaagttg ccacttgttt
     2101 ttaaaaattt cagattattg ggtctttctc tatcaatgaa ctggataata ggaccgattg
     2161 tgatgtttct tctggcgatt ttatttcttc acaatcatcc cggacttatg gttggggtga
     2221 ttcttgtagg tcttgcaaga tgcattgcaa tggtacttgt atggaacgac cttgcaaagg
     2281 gggacactga acttgcagct ggactggtag ctttcaatgc acttttccag gtgttctttt
     2341 actcggtata tgcatatgtt ttcattaccc ttttaccacc tcttttcgga ttgaagggtg
     2401 agattgtgga ggtttcgata tctgaaattg cagttacggt tcttatatat cttggtattc
     2461 cttttttcgc cggacttata acgaatatca tcctaagacg tacagcaggg gatgaatggt
     2521 attacaggaa atttatccca aaagcatcat ggctgactcc gatagccctt ctttttacga
     2581 tttttgtaat gttttccctg aagggagaaa aaattattga actgcccctt gatgtggtga
     2641 gagtagccat tccttataca atatactttg ccgtaatgtt tttctccaca ttttttattg
     2701 ctaaactgat gggatcgggt tatgaaaaat cgactactat ggctttcacc gcaggtagca
     2761 atgattttga actggcaata gcagtggcag ttgcagtgtt cggtatcgct tctcctgctg
     2821 catttgcaac ggtaattggt cctcttgtgg aagtgccggt tttgataatg ctcgtgaatg
     2881 ttgcactata tttcagaaaa aagtattttg ctgaagcata aagaatagtc tgcattaaag
     2941 tatttttact tcattcaatc tttgctctta tcgttaaaga gagggggtat taatttctat
     3001 ataattttaa ggttgtttaa tattccattt tgttagtctt gttttcgtaa ataattctga
     3061 aattaggagt gaaaatattt ttaaccatga gaaataagtt ctgtttaaaa tcggcggtca
     3121 atggaattgt aacactgatt cttttctttg ctctgagtgg ttgtaaagga aagagcgatt
     3181 ctttctcgtt tgtttttatg agtgacattc acctcaccta cgaaagaaat gcaattccgg
     3241 gttttaaaaa agccattgaa gaggtcaata aacttaaacc cgattttgtt attacaggcg
     3301 gtgaccttat tatggatgcc ctatcgcaaa gatatacaat ggctgattct ctttataacc
     3361 tttattctga aatggtaaaa gagattaaag ctcctgttta caacactatg gggaatcacg
     3421 agctttatgg tatatacagc cgcagcggtg ctgacccatc taatccggaa tacggagaaa
     3481 agatgttcga aaaaagattg gggaaatcat attactcatt tgactataaa ggctggaaat
     3541 ttttagtgtt gaattcagtg gaagacactg gaaaagacag atatataggg tttattgaca
     3601 gtacacagat aaactggatt aaagaggaac tgcagaaaac ggatacactt acaccagttg
     3661 ttctggtaac ccatatgccg tttatcacgg ttaactctca aatgtacaga tattctacag
     3721 catcaaacga ttccggcctt gtggtggtca attccaggga agtgctggat cttttcagcc
     3781 attataacct gaagcttgtt cttcagggtc atctgcatat ggttgaggat atatacatag
     3841 atggcataag attcattacc ggaggagctg tatcagcaaa ctggtggaga gggccttacc
     3901 gtggtgatga ggaaggattt atccttgtaa atgtgaaagg tgatgatttt acatggaaat
     3961 ttatagatta cggatgggaa gtacaaaaat gaaatatgtc tgctgagaaa tatgaatatc
     4021 aggccatgct gaaatcagtg atgaatttca gggatgcagg tgattcagac cctgcaggtg
     4081 gtaccggact gagaaaaggt ctgatttaca ggtctggtag tcttgacagg ataaaacggg
     4141 aagacattag aaggttgcaa cagcttggta tcaaggccat tgttgatttg agagctcctg
     4201 ctgaatatag taaaagaaaa gttgtgatac cggggattaa tgtgttttcc atgccacttg
     4261 attttgagta cgttacacgt gaaaggctga agccccttat aatgagcggg aatcaggagg
     4321 aagcagttca gaaagtaata actgaactat atcgagagat tcttgatgct gcaataccaa
     4381 tttacagaga aattgttgaa atgatactag acggagggca taccccgatg cttatacatt
     4441 gccgggcagg aaaagaccgg acaggtatca tatgtgctct tcttcaaatg gctgcaggta
     4501 gcagcgaaga ttcaattatt cgaaatttta tgaaatcgaa tgatgtaatt gtgccctact
     4561 accggaaaag gctctggata cgaaaaatat tatctctcgg gttttttcct gctgatgcca
     4621 ttcttgatgc cattaccctg cgtggagaaa atatcagaga agttattaag agagtcaggg
     4681 tgcattatgg cgggattgct ggttatttat cggtttccgg atttgatatg tcgaagttta
     4741 tgttattgaa aggcaaattg aataacatat aaaattatga tggtacaggc aaatgatgaa
     4801 aaagtcaggg aggcttcaga aataaaaaaa aggatttaca gtttcagaaa tgctttcaga
     4861 gggatttgga cagtattcag gtttgaaaaa aatttcagga ttcagtttat aattttttta
     4921 ttggttattg ctgcaggtat tgtccttgac atatcagtgg tagggtggat tgctgttata
     4981 attgtatcgg ggctggtatt tgccagcgaa tgttttaata ctgctgttga gtatcttggt
     5041 gatgcaataa ctgaaggaga agacataaat attaagaaag caaaagatgt tgctgctgca
     5101 ggagtattga tttcagcaat tatttcagtt ataacaggtt ttattgtttt tctaccagct
     5161 gtattaaggt ctctgggttt acgggtttag gtgatgtttt atctgtaagt ctttaatttt
     5221 cataaaacat aactaattga attgcattat tttatcaaat agtaaatatt tctaaatgat
     5281 aaaatatatc agttgataaa attttttaaa tgataaaaaa tactaactag tagttattat
     5341 tatttgataa aatttcttat ccgtcgaatt aacttcctca tatttaatta caaaataaac
     5401 tccagtctga ataaaccttt tgatttataa attaaccact aattccaata ctaccagaat
     5461 tgaaagatgt ggaaatattt aacttttcag tgttatgaaa taaatttgtg taaaattatc
     5521 gtgctatgac acttaaattt atccttcttg ctgttttcgg aattgcaatg gcacacctcg
     5581 aaggagtagt ggtagtctat ctgagaaaag caattggaat cctcgactcg gattccaaca
     5641 aggaggctgt taaccagatt aatccatggt atctaaaagt tgaaatgacc cgtgaagccg
     5701 caacaataat aatgctggca gttattgctt atcttacagg tacttcatgg gttgagaaag
     5761 gtatattctt tctctggact tttgccttct gggacctttt ttattacctg tcacttttta
     5821 tactgataaa atggcctccc tcattgaata caattgatgt actgtttctc atacctaaac
     5881 catggattgc cccggtatgg ttcccgatag gagtttcatc actgacaata atagttatcg
     5941 caatactcta tttatttaga attattccat gatatcaact tggagattgt gaattattgc
     6001 gacgttatct cgtaaaccac actggtaaga ggtgttgccg agccgatact gagaccggtt
     6061 ttcatcagat aatcacctgt atagctgcgg ccgctgtcag tgccctgacg gcgtaaccca
     6121 ggcataatat ttatctcttc tatcctgtaa gttttttcag ggtcgagccc cagatgcgct
     6181 gcaggtagta aaaaaagaga agtaaagcat caggtttata atgctgactg gaaaaaataa
     6241 ctttgaagat ttcataatat taatatttga cttaaatgaa caacaaattt aacaatatta
     6301 aaaggtagat aaatctgctg ggcttgcgtt agaaaaatta aaaattttta aaggggttaa
     6361 ttaattgtaa aaaagtcatc atataaacca tttgcaatat atatttttat aagcttaagt
     6421 ttatagctca ttgcttatga aaaaaataat agcccattta ataatcatac tgttcacggc
     6481 gactttattg ccgtttcaaa agctatatgg tgcgtttcag caatatgaag cagcaccgca
     6541 cgatacaata aagaagctta aaacctattc aggtcagcct gtttatataa catcccgtct
     6601 cgtcacctcc cggcctgtga tagatgggaa gcttgatgat gaatgctgga aacacggtaa
     6661 ctgggcaggg gattataccc agtttatacc caatgaagga gcaaagccaa catatcctac
     6721 tgaatttaac atacaatatg atgataagta cctttacata gcattcagag cgtacgatgg
     6781 tgaaccggga aagattcaga aatttgccgg tgcacgcgat gaaacagtgg gagatatggt
     6841 gggagttacg ttcgacagtt atcgtgatta ccggactggt tttgaattta caatcactgc
     6901 atggggtcaa aaggtagatc ttgtgttgtt taatccgatg aactgggatt tcaactggaa
     6961 tgctgtatgg aaggggaaga caggtctgga agattcggca tgggtggcag agcttgaggt
     7021 ccctctttct cagcttcgat acagtaacca gtatgaacag gtatggggga tgcacacatg
     7081 gagatggatt aacaggctcc aggaggagag taactgggag agacagtcca aaacgggtcc
     7141 aggtatgctg tacaattttg gtgaacttag aggaataaat ggattgaaga aatcgagacg
     7201 acttgaaata atgcctttta tgcttggaaa ggttgaaacg atgaagacgg aacccggaaa
     7261 tccttttact gcaaacggga aaatatgggg aggcaatgcc ggactggatg caaaaatagg
     7321 cgtttcaagc aatttcacaa ttgacctgac tgtcaatccc gattttggtc aggtcgaatc
     7381 agatccttca gtgatgaacc tgaccgcttt tgaaacattt tatgaagaaa agcgtccatt
     7441 tttcctcgaa ggattaacaa tatttgatta taaatttgat gacaaaacac tcttttattc
     7501 acgaagaata ggccattcac catcactctt aattaaaggc ggggatgatc ttttcgtcaa
     7561 aatgcctgat atgaccacca tattaagtgc tctaaagttt tctggaacaa cttcaaaagg
     7621 gctctcttta ggttttatac aggcactaac gtcagatgag tatgcaagag taagtgacgg
     7681 cggaggaaat aggagcagaa aaatggttga gccgctgacc aactacatgg ttgcaagggt
     7741 tcagaaaggc tacaatgagg gtgcaacagt tctaggtgca atgatgacat caaccagcag
     7801 gtttattaaa gatcccgagc ttgaatttct ggcaagaaac gcaattactg gagggctgga
     7861 tctgatccac cggtggcatg ataaggaatt ttacattgat gcccggctgc tgggaagttt
     7921 tgttgaaggc agtcaacgtg caataagaga actacaggaa tcttcggcgc gttattatca
     7981 aagacccgga gctgattatc ttgattacga tactttgcgt acaagcctga gcggtttcgg
     8041 tggaaaattc agaataggga aaggagcaaa aggattctgg agatattcaa caggcgtgtc
     8101 ctggctttct ccaggacttg aacttaacga tcttggttat atgagaacaa gtgatgaaat
     8161 aaaccaggaa aatgaaatat catattttgt taaccagcca gtatcagttt tcagaactta
     8221 tacaataact cttgaacaat ttaattcatg gaattttggt ggaatttacc tgggctcggg
     8281 aagccatctc tcatttgaat cggaatttaa aaataacctg gctttaagca caaattttat
     8341 aattcattcc ggttcatccg atcctagact tctaagagga ggatctgatt tcaaagttcc
     8401 ttatagcgta atgtcatttg gcaagataag aacaaaccct gcaaaaaagt taataatttc
     8461 tttaaactat gattatagtt attcgggaca tgattccagg actaattacg aaatcacccc
     8521 cggtatttcc ctgagacctt ttaatgccct cagatttgaa atgactgcat ccttcgggaa
     8581 gaacaaggat gagcttcaat acgtaacaac tttgaataca aatgaaggta aacgttatat
     8641 tctcgggacg attgaccagc agacactcaa tctgactttc agggtagatc ttaatattac
     8701 acctgaattt tcggttcagt attatggtag tccatttgta tctaagggac ggtattcaga
     8761 atttaaaaag gttacaaacc ctctggcaag aaattattca gacagatttt ctgtttttga
     8821 gaatcctgta ctgtcaggaa atatgtactt ccttgatgat aatggtaatt ttatacccga
     8881 ttattcaata gagaatcccg atttcaattt tcatcagttc cgctccaacc tggttgcaaa
     8941 atgggagtac aggctgggat catatattta tcttgtatgg tcgagtgacc gtacaggttt
     9001 tatggatcag cccgcttcat caatcggtga atcattccgc cagctatgga aagtatttcc
     9061 gaaaaatatc tttctaataa aattaaatta ctggttctct ttgtgatgct acggttaaat
     9121 aaaattcagg agaggtataa ctatttataa tataaagaga atgggaatga aaaggcttga
     9181 catagctgcc agaattaatt taatattccg ttcgctccga tacagaaatt acaggttgtt
     9241 ttttggaggg cagagtattt cgcttattgg tacatggatg cagcggatag ctttgccgtg
     9301 gatggtttac cgtatgactg gatcggcatt tatgcttgga ttggttggtt ttgttggtca
     9361 gattccgact tttatacttg cacctgttgc cggggttgta accgaccggt ttcacaggta
     9421 cagggttctt attataactc aaatagtttc gatggcgcag gcatctgtgc ttgcatatct
     9481 gaccttatct ggtgatatca aactctggca tataattgta ttgagtatta tccttggttg
     9541 cgtaaccgct tttgatgtac cttcaagaca ttcttttgtt gtggatatgg ttgagaaaaa
     9601 ggaagatctg ggtaatgcaa tagctcttaa ttcaatgatg ttcaacggtg cacgtcttat
     9661 aggtccttca attgccggtg taattctggc agtgaccagt gagggcatct gttttttatt
     9721 gaatgctatc agttttattt tcgttatagc ttcactttta atgatgaaag tggatggtaa
     9781 aaagaaatct gcaacaaaat cgggattatt ttctgacttg aaagaaggat ttctttacgc
     9841 attcggtttt gctcccatta agcatatcat tttactgctg gcacttgtaa gcctaatggg
     9901 tacctcctat caggtactta tgcctgtata cgccaaagaa gttttaaaag gaggttctga
     9961 aatttttgga tttctgatgg gagccgccgg actcggggca tttcttgcag ctctttatct
    10021 tgcatcgcgt gaaacagtta ttacacttgg aagaataatt cctctggcta cggggatagc
    10081 aggtgcagct cttactatta tttctcttga agatcttttc ttccttacat ttttcctgat
    10141 ggtttttgtc ggccttggat taatgcttca cacagtatca agcaatacaa ttctgcaaac
    10201 tattactgat gatgacaaaa ggggcaggat tatgagcttc tatacaatgt caataatggg
    10261 tattgcacct ttcggaagct tgattgcagg cgcaatggca aaggcacttg gaacgcgtct
    10321 gactttattg attgggggta tttcctgcct tgcaggggca tattttttct accgcaaatt
    10381 acctttgctt aaaaaaattg ccagacctaa atatgaaaaa atgggcatga tacctgaaat
    10441 tgtgactggc atacaaattg cttctgaacc agatcttaaa actgaagaat aattttgaag
    10501 caataggaat tgcaaacact tttataaatt ggtttgagtt tttctcagga tatatgagtg
    10561 aagaattaga tgacttccag attttcgtaa agccaatcgg tgcaaaatgc aatctgcatt
    10621 gcagttattg ttattacctg aacaaagaga acctttatgc ttcggaaagt tataaaactt
    10681 taccggagga tattctggaa gaatatatca ttcagcattt tgaggccagt tcgggcactg
    10741 atgttttctt ttcatggcac ggaggagaac cattgctggc gggaattgaa ttttatagaa
    10801 aagccattga actgcaaaat aaacataaac ctgaatcttt tacggtttat aatggtatcc
    10861 agacaaatgg tattctgttg aatgataaat ggtgcaggtt tctggctgga aatgattttt
    10921 acgttggtat cagcctcgat ggtcctgcag aatttcacaa tgcattcaga gtttacagag
    10981 acggtaggtc gtcttttgat aaagcccttg aagggtacag attattgcag caatacaata
    11041 tcagaaatga gattctatgt gtggtcagtt cagtaaatgt aagaaagcca ctcgaaattt
    11101 acagattctt taaaagtctt ggagtaaggt tcctgacttt cctgcctctt gttatcaggg
    11161 atatttcaaa accgggacaa gtttctgaca tttctgtacc ggctgaggaa ttcgggcagt
    11221 ttttgtgtaa aatattcgac gaatgggttt tggaagatat agggaggatt aagatacaga
    11281 ttgttgagga ggctatcagg cctgcattcg ggcaggagca ttcgctttgc atctttaaaa
    11341 aaatctgcgg gagggttcct gttgttgagc ttaacggaga cttctattcc tgtgaccatt
    11401 ttgttgatca tgaacatctt gtgggaaata ttatgaaaca atcagtttca gacctcctga
    11461 actcggataa acagaaaaat tttggaatgg caaaactgat tactcttccg gaatattgca
    11521 ggcaatgcga tgttagagaa atgtgtaatg gtgagtgccc taagaacaga tttgtaactg
    11581 ctcccggagg tgaacccggg ctaaattacc tgtgtgcagg ttacaggatg ttcttcaggc
    11641 acgtaaagcc atttgccgaa gctgttgggg ctgaatggag gcgtcggaac aggtaatatc
    11701 tatttcagtt tctttttgaa gaactccacg gttcgttgcc atgccagttt ggctgcttct
    11761 tcattatatc ttggacctgt gtcgttattg aaagcatggc ttgttccggg atacatataa
    11821 atataatgtt caatatttgc ctttttcagt gcctgttcga agttctgaat tcctgcattg
    11881 atccccggat cattttcggc atagtgagcc attatggcag ctttgatttt cggcacatcc
    11941 tcaactgcag gctggcgtcc gtaataaggc actgcagctt tcagggtggg agcatttaca
    12001 gcaacctggt tggtcatagc accaccccag cagaaaccgg tacaccctac cttgccatta
    12061 gaattgggat gggtttcaag gtatttgact gctgcaacaa agttcttaac cgtgtcatcg
    12121 ttgttgagtt tcttgaacag ctcaccaaca ttgctaacgt cgtcgggtgt tccgccaacc
    12181 ggtgaaagtg catcgggtgc gagagccaga aatccttcct tcgccattcg cctggtcaca
    12241 tcctgtatat gaggcaccag ccccctgttt tcatgtatca caattactgc agggtttttt
    12301 cggggcttaa ccggcctggc aaggtaagcc ctcatatcgc aattgaatcc aggatatttt
    12361 atgaattcag ttaccagtcc gggaaaatcc tgatattcag ccagagcagt gtgctcatca
    12421 ccaagttcag ggagtattgc tagggcggcc gtaacgctgc cagtgtacaa tataagcttc
    12481 ctcagaaatt cccggcgcga agtttctccg cgtttgtatt cctcatataa agacttaatt
    12541 tctttttcca taaccttaaa tttttaaata ttggtacaaa attccatatt gaattaaaac
    12601 attgttaaaa tattaaataa atggaacatg atgaaatttt taatatattt taaatatttt
    12661 ttctgtcaga ataaatttta ttatcttgga aagtacgttg aaacaattct tattaaataa
    12721 cccgttaaat aattattttg taaaatatgc cgaaaagtgt actcaaaatt catgcaagca
    12781 atactgataa aataggcaat gagcttt
//
LOCUS       DAOSNB010000033        12706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_4937_length_12706_cov_5.078729, whole genome shotgun sequence.
ACCESSION   DAOSNB010000033 DAOSNB010000000
VERSION     DAOSNB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4937_length_12706_cov_5.078729"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..380)
                     /locus_tag="PLG42_02645"
     CDS             complement(24..380)
                     /locus_tag="PLG42_02645"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOU30141.1"
                     /translation="MKEAMKHREHHPAIGIGLFFIVLGLALLVATNDLLNLGSTSEYF
                     TWQTALIFIGVLLMLNFRVVGGIILISIGTWFFLQHYYFEIPHTLEVMYWPAVIIIIG
                     VFFLLTSLVGRKKQNS"
     gene            555..1076
                     /locus_tag="PLG42_02650"
     CDS             555..1076
                     /locus_tag="PLG42_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOU30142.1"
                     /translation="MTGLYVIPLFGLSQGHHSFDFEIGNTFFDGFEESEIKEGELRAV
                     AGIDKSPTCIDLTIKITGSVKVCCDRCLDMYMQSIECENHLIIREGNSYDDSDPDILF
                     VPHNEKELDISQLLYEYIHLALPLRRIHPDDSEGHSGCNPEMIKELNKHIINQASSED
                     SRWNELKKLLNNN"
     gene            1088..1273
                     /gene="rpmF"
                     /locus_tag="PLG42_02655"
     CDS             1088..1273
                     /gene="rpmF"
                     /locus_tag="PLG42_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOU30143.1"
                     /translation="MAHPKHKHSKTRRDKRRTHYKAVKPTISVCSNCGSTVEYHRVCP
                     ECGYYRGKPAIEKKLAS"
     gene            1298..2236
                     /gene="plsX"
                     /locus_tag="PLG42_02660"
     CDS             1298..2236
                     /gene="plsX"
                     /locus_tag="PLG42_02660"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOU30144.1"
                     /translation="MRLGLDVMGGDYAPDVVIEGAVDTLGSLSADDKIVLIGNENLIL
                     DKLAGLGVDPLLFEIVHASQVIEMGEHPAKAYSQKKNSSIAVGFGLLKSGLIDGFCSA
                     GNTGAMLVGASYTVNVIPGVLRPALITKVPSITGKYSVMLDVGLNPDCKPDVLLQYAR
                     LGSIYAHHILGIENPTVGLLNIGSEETKGTSAVRAAYEMMKEDPQLNFHGNIEGNSLF
                     HDGMTDVIVCDGFVGNIVLKQAEAFFTIYRKRNLKDSFFELLDFENIGGTPVIGINGN
                     VIIGHGNSSRKAIKNMILQTKSVVEVNLAQKIKEEI"
     gene            2246..3244
                     /locus_tag="PLG42_02665"
     CDS             2246..3244
                     /locus_tag="PLG42_02665"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOU30145.1"
                     /translation="MNKIRAAITGVHAWVPEYRLDNFELSRMVDTSDEWIMQRVGIKE
                     RRILKGEGLGTSDMADKAVAGLLEKTGTSPDEVEIIICATITPDMFFPSTANIISDKV
                     GIKNAFGFDISAACSGFIYALHIGASFIETGRYKKVVIVGADKMSSITDYTDRTTCPL
                     FGDAAAAVLLEPTTEEVGVMDQILRSDGSGRKYLYLKAGGSVRPASHETVDAKEHFIY
                     QEGQTVFKFAVTYMADVAVEIMQKNNLKPEDIAYLVPHQANLRIIEATGRRMGLPMEK
                     VMINIEKYGNTTAATIPLALWEYESRLKKGDNLILAAFGGGFTWGAVWVKWAYNPK"
     gene            3291..3695
                     /locus_tag="PLG42_02670"
     CDS             3291..3695
                     /locus_tag="PLG42_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOU30146.1"
                     /translation="MAKNNNEAENPTINLISQGTEITGDIKSNGDIRIDGSLNGNLYT
                     KGKVVVGNTGKIKGEVTCKNSEISGTLEGKITVNQLLNLKASSKIIGDVVTSKLAIEP
                     GAIFTGTCKMNETEYNAGQNNTGGPEKPAEKR"
     gene            3643..3897
                     /locus_tag="PLG42_02675"
     CDS             3643..3897
                     /locus_tag="PLG42_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOU30147.1"
                     /translation="MQDKTTPEDQKNQQKKDRFEGLHNYAKYSGLAFQMIAIILITVW
                     AGTKLDKLFLMETPVFTIILSLFGVAAAIYTAVKDFIKIK"
     gene            complement(3890..4279)
                     /locus_tag="PLG42_02680"
     CDS             complement(3890..4279)
                     /locus_tag="PLG42_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30148.1"
                     /translation="MFFNDFNIIKIPVIEKAKYSIKNNKISFHPVFFIRANHIKASTN
                     SKTNFRETEINECMVSSRGLFCRPLKKTKREMVVAKKNIPEIEGKCQALNFVLKTEIM
                     INQMMFSSASRKKSSSRYFRKFFKFST"
     gene            4292..5380
                     /gene="atpB"
                     /locus_tag="PLG42_02685"
     CDS             4292..5380
                     /gene="atpB"
                     /locus_tag="PLG42_02685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOU30149.1"
                     /translation="MNKTLFTISVLLFILFQSWSPLAGQEIRSEISQSEKTEEISENE
                     FNASEFIIDHITDSHEWHIMTLGHNKHVTIYLPVILWSKEKGLSVFSSKKLVHGNIYK
                     GYKLEEEGDLKGKIVQIKSDGTIDEENLPLDLSITKTVAGMLAAAIIGLIIFISLAQS
                     YKKRGISHPRGLQGFLEPVILFVRDDIAIPNIGHEKYEKYMPYLLSAFFFILINNLMG
                     LVPFPPPFGANVTGNIAVTMVLAVFTFLITNLSGSKSYWRHIFATPGVPFWLLPIMIP
                     VEIIGMFSKPFALMVRLFANISAGHIIVLSLIALIFIFNSVWVAPVSAVFVIFMDCLE
                     TLVAFLQAYVFTLLSALFISLAVKEEHH"
     gene            5409..5615
                     /gene="atpE"
                     /locus_tag="PLG42_02690"
     CDS             5409..5615
                     /gene="atpE"
                     /locus_tag="PLG42_02690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOU30150.1"
                     /translation="MTGTLAAIGAGLAVIGAGLGIGNIGKAAMEAIARQPEATSKIQT
                     AMIIAAALIEGVALFGVVVSLIAK"
     gene            5697..6209
                     /gene="atpF"
                     /locus_tag="PLG42_02695"
     CDS             5697..6209
                     /gene="atpF"
                     /locus_tag="PLG42_02695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOU30151.1"
                     /translation="MILASSLASPEIGTIFWTTLIFALFFIVLAKFAWKPILNAVKAR
                     EEIIKSSLEAAEKAREDMKRLQADNEAILRKAREERENILKEARETREKMIAEAKTKA
                     SEEAGKLIEKARQEIEREKLKALSEIHSQVAFLSVEIASKILGEKLKETEEQEKLIGR
                     YLKELDKNTN"
     gene            6224..6769
                     /gene="atpH"
                     /locus_tag="PLG42_02700"
     CDS             6224..6769
                     /gene="atpH"
                     /locus_tag="PLG42_02700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOU30152.1"
                     /translation="MNEGKIPVRYAKALFESAVNKNLISNIYEDMQLIGETCKQEEVR
                     ELLRSPIIPASEKKKIFHNLLANKINPLTLSLIDLMIKNGRESFLPSVARILSANTLR
                     YQGITETNLITAVPVSERVRKQITDYISEKFKTKVSLKENVDPEIIGGFILKVDDYFI
                     DASIKTRLRKIKKSLIGNAQQ"
     gene            6794..8371
                     /gene="atpA"
                     /locus_tag="PLG42_02705"
     CDS             6794..8371
                     /gene="atpA"
                     /locus_tag="PLG42_02705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOU30153.1"
                     /translation="MASIKPAEVTKILREQLEGIQTGVELEEVGVVLQVGDGIARIYG
                     LTNAQSNELIEFENGIKAIVLNLEEDNIGAVLLGPSEEIKEGDIVKRTGRIASIMVGE
                     GLLGRVITPTGEPIDGKGPITGELYEMPFERKAPGVIFRQPVKQPLQTGLKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRSNYEKGEPVYCIYVAIGQKGSTVANIAAT
                     LESYGAMDYTVIVVATASDPAASQFYAPFAGCAIGEFFRDTGRDALIIYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDIFYLHSRLLERAAKIIESDEVAQKMNDLPPSIRHMVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESGLFNAGVRPAINVGISVSRVG
                     GHAQIKSMKKIAGTLKVDQAQYRELEAFAKFGSDLDAATLAILDKGAKNVEILKQGQF
                     SPMPVEKQIAIIYCGTRGLLKDVPVNKVKEFERDYLEYLENNHRDVLDELAKGNINDR
                     ITDTLDKAAAEIAMRFK"
     gene            8398..9282
                     /gene="atpG"
                     /locus_tag="PLG42_02710"
     CDS             8398..9282
                     /gene="atpG"
                     /locus_tag="PLG42_02710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOU30154.1"
                     /translation="MPNLKAIRLRINSVKSTRQITSAMKMVAAAKLRKAQDKILRLRP
                     YAGKLNEILVGLTESLANTEEDNIYGRKSEPEKILIVVITSNRGLCGSFNSNVIRETR
                     RVIYEDYYEQYKNGNLWFIAIGKKGYDFLRKQKLNILEEANDLVNNLTFEKSVPLATR
                     IMEHFVSGRFDRVRIIYNQFKNAAVQILTNESFLPVETKTTEKKIHPVVDYIYEPTKE
                     EIIKDLIPRTLKIQFYKAILDSFVAEHGARMTAMHKATDNATELIKDLTLQYNKARQA
                     AITNQILEVVTGAEALKG"
     gene            9319..10197
                     /locus_tag="PLG42_02715"
     CDS             9319..10197
                     /locus_tag="PLG42_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU30155.1"
                     /translation="MYNQTQLKDLVEKALLNLSLPCEAPRLAETIRYILSAGGKRLRP
                     VLALMACNIFTDKIDHAVLPAAGLEIFHNFTLVHDDIMDNAPVRRNLPTVHTRWNVNQ
                     AILSGDIMAFIATECFINLPPEILQTVLRIYNKAAIDVCTGQQLDLDYEKVPSVTESE
                     YLRMIELKTASLIAASLAIGATLGGAGTKERDTLHEFGVNLGMAFQIQDDLLDVYGET
                     RIFGKTSGGDIAAGKKTFLYIKAMEMATGELLKTLKSLYQDKNNNRAEKIIIVKEIYD
                     TLNIPQITENLANQYI"
     gene            complement(10330..10899)
                     /locus_tag="PLG42_02720"
     CDS             complement(10330..10899)
                     /locus_tag="PLG42_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30156.1"
                     /translation="MKKYSFFLLVLLYMNCGIIYCQSNLWAGAGPFYTTGFYFLNNRL
                     ESYKTTNAGVFLKGLYNISNQFHVSPSLLWVFPNIYKISVVDGEERTSVSSIMFDING
                     HYVIKSMGPFEFYGLAGLDILFARRKYVSTILNAEQKTIVTDNTPGLNLGAGTLVQAG
                     EKVKLFAEIKYLLSKYDQLMLNVGFLINL"
     gene            11051..12559
                     /gene="atpD"
                     /locus_tag="PLG42_02725"
     CDS             11051..12559
                     /gene="atpD"
                     /locus_tag="PLG42_02725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOU30157.1"
                     /translation="MARNIGRISQIIGPVVDVVFDQQGAEMPEIYDALEIKRDDGSIL
                     VVECQQHIGENTVRTIAMDSTDGLRRGMEVVATGAPIVMPIGEQIKGRLMNVTGDPID
                     GLGPLDKKGGYPIHRDPPKFEELSTEKEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVIILELINNIAKAYSGLSVFAGVGERTREGNDLLREMIEAGVINYGEAFLESMEKG
                     GWDLTKVDMEALKESKLALVFGQMNEPPGARARVALSGLSIAEHFRDGDGKSGGRDIL
                     FFMDNVFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKHGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKISELGIYPAVDPLDSTSRILTPEIVGVEHY
                     RCAQRVKEILQRYNELQDIIAILGMDELSEEDKLIVHRARRVQRFLSQPFHVASQFTG
                     IPGVLVSIEDTIKGFNMIIDGKVDQYPEAAFMLVGTIEDAIEKGEKLLAQSR"
BASE COUNT         4259 a         2507 c         2595 g         3345 t
ORIGIN      
        1 atgatcataa aattatttgt tttttaacta ttctgtttct ttctgccaac caacgatgtt
       61 agaaggaaaa ataccccgat aataattatt accgcaggcc agtacattac ttcaagtgtg
      121 tgaggaattt caaaataata gtgctggaga aaaaaccagg ttcctatcga aataagtatg
      181 attcctccaa ctactctgaa attcaacatt aatagaacgc ctataaaaat aagagctgtc
      241 tgccatgtga aatattcaga cgtgcttcca aggtttagca ggtcatttgt cgcgacaagc
      301 aaagccagtc ccaggacaat aaaaaacaat ccaatgccaa tggctggatg atgttccctg
      361 tgtttcatag cctctttcat tttatatcat ttcaggcaaa aatattaatg gaagaacttc
      421 agctaaataa gaaatcggta aatatcctaa ttttgccggc aaattgcagg aaaaagttgt
      481 aaaggatact ttttcattca taattttttt tattgaacaa ggatttttaa ttttgcaaac
      541 caaaatttaa aataatgacc gggttatatg tgatacccct gttcggtctg agccaaggtc
      601 atcacagctt tgactttgaa atagggaata cgttctttga cggtttcgag gagtcggaga
      661 tcaaggaagg agaactcagg gcagttgccg gaattgataa atctcccacc tgcattgatc
      721 tgaccataaa aataaccggc agtgttaaag tttgctgcga cagatgcctt gacatgtata
      781 tgcaaagcat cgaatgtgag aaccacttga taattaggga aggtaacagc tatgacgaca
      841 gtgatcccga catcctattt gtacctcata atgagaaaga actggacatc agccagcttc
      901 tttatgaata tatacacctg gccctgccct taagaaggat ccatcccgat gacagcgaag
      961 gccacagtgg ttgtaatccg gaaatgatta aagaacttaa taaacacatt ataaaccagg
     1021 cgagcagtga agactcgaga tggaatgaac tgaaaaaact tttaaataac aattaaagca
     1081 ataagaaatg gcacatccga aacataaaca ttccaaaaca agaagggaca aacgcagaac
     1141 tcattacaaa gccgtaaagc ccacaatatc tgtatgttca aattgtggat cgactgtaga
     1201 atatcacaga gtctgtcctg aatgcggata ttacaggggc aagcctgcca ttgagaagaa
     1261 actggcatcc tgaatagacg gcacaaacta agataccatg aggctgggcc ttgatgtaat
     1321 gggaggtgat tatgctcctg atgttgtaat agaaggagct gttgataccc tgggcagttt
     1381 atcggcagac gataaaatcg ttcttatagg caatgagaac ttaatacttg ataaacttgc
     1441 cgggctagga gttgacccgt tattgttcga aatagttcat gcctcccagg taattgaaat
     1501 gggggaacac cctgctaagg catattccca gaaaaaaaac tccagcatag ctgtaggatt
     1561 tgggttgctc aagagtggcc tcattgatgg cttctgcagc gccggaaata caggagcaat
     1621 gcttgttggc gcaagttata ccgtaaatgt tattccaggt gtgctgagac ctgctcttat
     1681 tacaaaagta ccctcaataa ccggtaagta ttcagtaatg ctcgatgtag gattaaatcc
     1741 agattgcaaa cctgatgttc tgctgcaata cgctagactt ggctcaatat atgcgcatca
     1801 tattcttgga atagaaaatc ctactgtcgg cctcctgaat attggttcag aagaaacaaa
     1861 gggaacttca gctgtcagag ccgcctatga aatgatgaaa gaagatccac agcttaattt
     1921 ccatgggaat atagaaggta actcactatt tcacgacggt atgacagatg ttattgtatg
     1981 cgatggattc gtgggtaata ttgtactaaa acaggcagaa gctttcttta ctatttacag
     2041 aaaacggaat ctcaaagatt cgttttttga actcctcgat tttgaaaata taggtggaac
     2101 accggttatt ggcattaatg gaaatgttat aatcggacat ggaaattcca gcaggaaggc
     2161 aataaagaat atgattctgc aaacaaaatc agttgttgaa gttaatcttg cacaaaaaat
     2221 aaaagaagaa atttaacact tctttatgaa caaaatcaga gctgcaataa ccggtgttca
     2281 tgcatgggtt ccggaataca ggcttgacaa ttttgaactc tcaaggatgg tcgacacatc
     2341 cgatgagtgg ataatgcaac gggtgggaat aaaagaaaga cgcatattaa aaggtgaagg
     2401 tcttggcact tccgatatgg ccgataaagc agttgcggga cttcttgaaa aaacaggcac
     2461 ctctcctgac gaagtggaaa tcatcatttg cgccacaatt acacccgaca tgtttttccc
     2521 ttccactgcc aacatcataa gtgataaggt tggaatcaaa aatgctttcg gcttcgatat
     2581 cagtgcagca tgctcgggat tcatttatgc cctgcatata ggagcatcat tcattgaaac
     2641 tggaagatat aaaaaagtgg ttattgtagg tgccgacaaa atgtcatcaa taactgatta
     2701 taccgaccgt acaacctgcc ctctgttcgg agatgcagca gctgcagttc tgcttgagcc
     2761 taccactgaa gaagtagggg taatggatca gatactccgt tccgacggat caggccgcaa
     2821 atacctgtac cttaaagcag gaggctcagt aaggcctgcc tctcacgaaa ctgtcgatgc
     2881 caaagaacat tttatttatc aggaaggtca aacagtcttt aagtttgctg taacatatat
     2941 ggcagatgtt gccgttgaaa taatgcagaa aaacaacctc aaaccggaag acatagcgta
     3001 ccttgtgcct caccaggcaa acctcagaat aatagaggca acgggccggc gaatgggatt
     3061 gcctatggaa aaagtgatga tcaacattga aaaatatggc aatacaactg ctgcaacgat
     3121 tcctttagcg ctgtgggaat atgaatcacg tctgaaaaaa ggagataacc tcatccttgc
     3181 agcattcgga ggcggattta cgtggggtgc tgtctgggtc aaatgggcat ataatccaaa
     3241 ataatcttat ctttgcaaag gagtaataca gattaataaa ataaaatatc atggcaaaaa
     3301 ataataacga ggcagaaaac ccaaccataa acctgattag ccaggggact gaaatcacag
     3361 gtgatattaa atcaaatggc gatatcagaa ttgacggttc acttaatggc aatctgtata
     3421 ccaaagggaa agtagtagtc ggaaatactg gaaaaatcaa aggagaagtt acatgcaaga
     3481 actcggaaat ttcagggaca ctcgaaggga aaataacagt aaatcaactt ctcaatctca
     3541 aagcctcctc taaaatcatt ggcgatgtag ttacttcaaa attggcaatt gaacctggtg
     3601 ctatatttac cggtacctgt aaaatgaatg aaacagaata caatgcagga caaaacaaca
     3661 ccggaggacc agaaaaacca gcagaaaaaa gataggttcg aaggacttca taattacgca
     3721 aaatattccg gccttgcatt ccagatgatt gcaataatac tgattaccgt atgggcagga
     3781 acaaagctgg ataaactttt tctgatggag acacccgttt ttactataat tctttcattg
     3841 ttcggagttg ctgctgccat ttacacagct gtaaaagatt ttattaaaat caagtagaaa
     3901 atttgaaaaa ttttctgaaa tatctactgc tgcttttttt tctgctggca ctgctgaaca
     3961 tcatctggtt tatcataatt tctgttttta atacaaaatt aagtgcctgg catttccctt
     4021 caatctcagg tatgttcttt ttggcaacaa ccatttccct ttttgttttt ttcaggggtc
     4081 tgcaaaaaag tccacgtgaa gaaaccatgc attcatttat ttctgtctcc ctgaagtttg
     4141 ttttggaatt ggtgcttgct ttaatatggt tcgccctgat gaaaaaaaca ggatggaatg
     4201 atattttatt attttttatg ctatatttag ccttctcaat aacaggaatt tttattatat
     4261 tgaagtcgtt gaaaaacaaa attttaaagg cttgaacaaa acattgttta caatatcagt
     4321 tctgttgttt atactgttcc agtcatggag tcctctggca ggtcaggaaa tccggtcaga
     4381 aatttctcaa tctgaaaaaa ccgaagaaat aagtgaaaat gaatttaatg caagcgaatt
     4441 cattattgat cacattactg attcacatga atggcacata atgaccctgg gtcataacaa
     4501 acatgttacc atttatctgc ctgtaatcct ctggagcaag gaaaagggct tatcggtttt
     4561 ttcttcaaaa aaacttgtcc acgggaatat ttataaaggc tacaaacttg aagaagaagg
     4621 agatctgaaa ggtaaaatcg ttcagataaa aagtgacggg actattgacg aagagaatct
     4681 acccctcgac ctttcaataa caaaaacggt agcaggcatg cttgcagcag caattatcgg
     4741 ccttattatt tttatttcgc ttgcccaatc ttataaaaaa cgtggtatca gtcacccacg
     4801 tgggcttcaa ggcttcctgg aacctgtaat tctttttgtc agagatgata tcgcaatacc
     4861 taatatcggg catgaaaagt atgaaaaata catgccctac ctgctttcgg cctttttctt
     4921 catacttatc aataacctga tgggacttgt tccttttcct cctccattcg gagccaacgt
     4981 aacaggaaat attgctgtga caatggttct tgctgtattc actttcctga ttacaaacct
     5041 gagcggcagc aaaagttact ggaggcacat ttttgcaaca ccgggagtcc cgttctggtt
     5101 actaccaata atgatacctg tcgaaataat cggcatgttt tccaagccgt ttgctctaat
     5161 ggtccgattg tttgcgaaca tttcagcagg acatattatc gtattaagtc ttatagcact
     5221 tatttttata tttaattcag tatgggttgc tccagtttcg gcagtttttg taatattcat
     5281 ggattgcctc gaaacactgg tagcattcct tcaggcatac gtttttacac ttctttctgc
     5341 actgttcata agccttgcag tgaaggaaga acatcattaa tatatgttta actaaaaatt
     5401 taattatcat gacaggaaca ttggcagcaa taggagcagg tcttgcagtt ataggagcag
     5461 gacttggtat tggcaacatc ggaaaagcag caatggaagc cattgcccgc cagcccgaag
     5521 ccacttccaa aatacagaca gcaatgatca tcgctgcagc acttatcgaa ggtgttgcac
     5581 ttttcggtgt tgttgtctct cttattgcga agtaagaaaa accggaagcc tgcaacggtt
     5641 ggttgcaggt ctttcatttt gaaaaacaat ctgaacaaac ataacattct gataaaatga
     5701 tacttgctag cagtcttgca tcacctgaaa taggcacaat attctggaca accttaatat
     5761 ttgcactctt ttttattgtc ctggcaaaat ttgcatggaa gcctattctg aatgcagtaa
     5821 aagcacgcga ggaaattatc aaaagttccc tcgaggctgc cgaaaaggct cgtgaagata
     5881 tgaaaaggct ccaggccgac aacgaggcta tcctgcgaaa agcacgtgag gaacgcgaaa
     5941 acattctcaa agaagcacgt gaaacacgtg aaaaaatgat agcagaagca aaaacaaaag
     6001 cttctgaaga ggctggaaaa ctgatcgaaa aagcacgcca ggaaatagaa cgagaaaaac
     6061 ttaaagccct ctctgaaata cattcacagg tggcattcct ttcagtcgag atagcatcaa
     6121 aaatactggg agaaaaactt aaagaaactg aagaacaaga aaaactcatc ggacggtatc
     6181 ttaaagaact tgataaaaac accaattaaa aggataccct gaaatgaacg aaggaaaaat
     6241 accggttaga tacgcaaaag cactgtttga gtcggcagta aataaaaacc tcatcagcaa
     6301 tatttatgaa gatatgcagc tgatcggtga aacatgcaaa caggaagagg ttcgtgaact
     6361 gctcagaagt cctattatac cagcttcaga aaagaaaaaa atttttcata atctccttgc
     6421 aaataaaata aaccccctta cactttcact gattgatctt atgataaaaa atggcaggga
     6481 gtcattcctt ccttctgttg ccagaatatt aagtgctaac acacttagat accagggtat
     6541 aaccgaaact aacctaatta ccgcagtacc tgtaagcgag agagtcagga aacaaattac
     6601 agattatata tctgaaaaat tcaaaacaaa ggtaagtctt aaggagaatg ttgaccctga
     6661 aattattgga gggttcatcc ttaaagtcga tgattatttc attgatgcaa gtataaaaac
     6721 caggctaaga aaaattaaga aaagtcttat tggaaacgca caacagtaaa taattaaaaa
     6781 acaaataaaa tctatggcca gtattaaacc tgcagaagtt acaaaaattc taagagagca
     6841 gctcgaaggc attcaaactg gtgttgaact ggaagaggta ggggttgtcc ttcaggttgg
     6901 agacggaata gcacgtattt atggtctgac aaatgctcaa tcaaacgaac ttattgaatt
     6961 cgagaacggg atcaaggcaa ttgtccttaa tcttgaagaa gacaatatag gcgctgtatt
     7021 gctgggtccc tccgaagaga ttaaagaagg agatattgtc aaacggacag gccgcatagc
     7081 ctcaataatg gttggagaag gactgctcgg aagggtaatt acaccaacag gtgaaccaat
     7141 cgacggaaaa ggtcctataa caggagagct atacgaaatg ccctttgaaa gaaaagcacc
     7201 gggtgtaatt tttcgacagc ctgttaagca accattacag acaggcttaa aagctatcga
     7261 tgcaatgata cctattggca ggggacagag ggaactcatt attggcgacc gtcagacagg
     7321 caagacagct attgctatcg acaccataat aaaccagcgc agtaactatg aaaaaggtga
     7381 gcctgtttac tgcatttatg tagcaatagg tcagaaaggt tcaaccgtag ccaatatagc
     7441 agcaactctt gaatcttacg gtgcaatgga ctatacagta atagtagttg ccacagcctc
     7501 cgaccctgca gcatcccagt tctatgcacc ttttgcaggt tgtgctattg gtgaattctt
     7561 ccgcgatacc ggtcgcgacg ccctgattat atacgacgac ctctcaaaac aggcagtatc
     7621 ataccgcgaa gtgtcgcttc tgctgcgccg tccacctggc cgcgaagcat acccgggaga
     7681 tattttctac ctccattcaa gacttcttga aagagctgcc aaaataattg aatcagatga
     7741 agtggcacag aagatgaacg acctgccacc ttcaatcaga cacatggtta aaggaggcgg
     7801 atcacttaca gcattgccaa taatagaaac ccaggcagga gacgtgtcgg cttatatacc
     7861 cacaaacgta atttcaatta ccgacggtca gatattccta gaatcaggtc tctttaacgc
     7921 aggagtccgc ccggcaatca atgtcggaat ttcagtatca agagtgggtg gtcatgccca
     7981 gataaaatca atgaaaaaaa ttgcaggcac acttaaagtc gaccaggcac agtaccgcga
     8041 attggaagca tttgccaaat tcggttccga cctcgacgcc gccactcttg caatactcga
     8101 taaaggagct aaaaatgtcg aaattcttaa acagggacaa ttctccccca tgcctgtaga
     8161 aaaacagatt gcaattatat actgcggcac cagaggactt ctgaaagatg tacctgtaaa
     8221 caaagtgaaa gagtttgaaa gggattatct ggaatatctt gaaaataacc acagagatgt
     8281 tctggatgaa cttgcaaaag ggaatatcaa cgacaggata actgatacgc tcgacaaagc
     8341 agcagctgaa atagcaatgc gtttcaagta acaatcaaaa cagaattata gcagaaaatg
     8401 ccaaacctta aggccatacg actcaggatc aattctgtaa aatctaccag gcagattaca
     8461 tccgccatga aaatggtggc agcagccaaa ctccgcaagg cacaggataa aattctccgg
     8521 cttagacctt atgcgggaaa attaaatgaa atccttgtag gtcttactga aagcctcgcc
     8581 aacactgagg aagataatat ttacggaaga aaatctgagc cggaaaaaat acttatcgtt
     8641 gtaataactt caaacagggg tctttgcggc agttttaact caaatgtaat tagagaaacc
     8701 cgccgggtaa tttatgagga ttattatgag caatataaaa acggcaacct gtggtttata
     8761 gccattggta aaaaaggata cgatttctta cgaaaacaaa aactcaatat ccttgaagaa
     8821 gccaacgatc tggtcaacaa ccttactttt gaaaagagtg ttccgctggc cacaaggata
     8881 atggagcatt tcgtaagcgg aaggtttgac agagtcagaa ttatttacaa tcagtttaaa
     8941 aacgctgctg tacagatact taccaatgag tcgttcctcc cggtagaaac aaaaacaaca
     9001 gagaaaaaaa tacatcctgt agttgattat atctacgaac caacaaaaga ggaaataatt
     9061 aaagacctta taccaagaac tctcaaaata caattctaca aagctatact cgattctttc
     9121 gtagctgaac atggcgcacg catgacggct atgcataaag ccaccgataa cgctacagaa
     9181 ctgataaaag atcttacgct tcagtacaac aaagcacgcc aggcggctat taccaaccag
     9241 atacttgaag tggtaacagg agccgaagct ttaaagggtt aaaatagttc tttaatacca
     9301 ttctgataaa catatctgat gtacaaccag acacaactta aagatttagt tgaaaaagct
     9361 ttgttaaatc tttcacttcc ttgtgaagct ccccgtcttg ctgaaaccat cagatatatt
     9421 ctctcagccg gaggcaaacg actgagacca gttcttgcat taatggcatg caatattttt
     9481 accgataaaa tcgaccatgc cgttcttccg gcagcaggcc ttgaaatatt tcataatttc
     9541 acactcgtcc atgacgatat catggataat gcacctgtca gaagaaacct gcctactgtc
     9601 cataccagat ggaacgtcaa ccaggctatc ctttcaggag atataatggc ttttatagca
     9661 actgaatgct ttattaacct cccgcctgaa attctccaaa cagttcttcg catatataat
     9721 aaagcagcta ttgacgtatg cacaggccag cagttagatc ttgattatga aaaagttcct
     9781 tcagtaacag aaagtgaata cctgagaatg attgagctaa agactgcatc acttattgca
     9841 gcttccctgg caattggagc aactctgggt ggagccggta ctaaagaacg ggacactttg
     9901 catgagttcg gagtaaacct cggaatggca tttcagatac aggatgattt acttgacgtc
     9961 tacggagaaa caagaatctt cggaaaaacc tcaggtggag atatagctgc cggtaaaaag
    10021 acattcctgt acattaaagc aatggaaatg gctaccggtg aattacttaa aacccttaaa
    10081 tccctttatc aggataagaa caataataga gccgaaaaaa ttattatcgt aaaagaaata
    10141 tatgatacgc tgaatatccc gcaaataacc gaaaatcttg caaaccagta tatataacgt
    10201 gcaatttcta cactaaaatc tcttcctgtt aataacgaca gaaaagaaga actaatgtcc
    10261 cttactcttt cattaacagg aagaaatatc taaaaaattc taagccactt tttattcttt
    10321 actctttaat tacaggttta tcagaaaccc gacatttaac atcaactggt catatttact
    10381 gagtaggtac tttatttctg cgaaaagctt aactttttca cctgcctgaa ccagtgttcc
    10441 cgctcccaga ttcaatccag gtgtgttatc tgtcacaata gttttctgct cagcatttag
    10501 aatggtcgaa acatattttc ttctggcaaa taaaatatca agccctgcca ggccgtaaaa
    10561 ctcaaaaggt cccattgatt taattacata atgcccgtta atatcaaaca ttatagatga
    10621 aacagaggtc ctttcctcac cgtcaacaac actaattttg taaatattag ggaaaaccca
    10681 taaaagagac ggtgaaacat gaaactggtt gctgatattg tataatcctt tcagaaaaac
    10741 cccggcattt gttgttttat atgattccag cctgttatta agaaaataaa atcctgttgt
    10801 ataaaaagga cccgcaccag cccataaatt tgactggcag taaattattc cacaattcat
    10861 atacaggaga accaaaagga aaaaagaata tttcttcatt tttaaagtta ttttagtcag
    10921 taattaaaat tttggcactt ccaaagatta catttcagga gttaaattac tctaaatttt
    10981 tcttttgaaa gaaaaaggaa agtaaaaatt attacatttg cctaccgtta agttacaaac
    11041 attttttatt atggctcgaa acattggaag aatcagtcag ataataggac cggttgtaga
    11101 tgttgttttc gatcagcagg gtgctgagat gccggaaata tatgatgctc tggaaataaa
    11161 acgtgatgat gggtcaattc ttgttgttga atgccagcag catattggtg aaaatacggt
    11221 gagaacaatt gcaatggatt ccaccgacgg ccttcgaaga ggaatggaag ttgttgcaac
    11281 gggagcacca attgtgatgc ccattggtga gcagattaag ggaaggctga tgaatgttac
    11341 aggtgatccc attgacggtt tgggtcccct cgataaaaag ggcggatacc ccattcacag
    11401 ggatcctccc aaatttgaag aactctcaac ggaaaaagag gttctctata ccggaataaa
    11461 agtaattgac cttatcgagc catattcgaa aggaggtaaa atcggtcttt tcggtggtgc
    11521 aggagttgga aagacagtta tcattcttga actgataaac aacattgcca aagcatattc
    11581 aggcctgtcg gtttttgcag gagtgggtga aagaaccagg gaaggcaatg accttctcag
    11641 ggaaatgatt gaagctggcg ttatcaacta cggtgaggct tttcttgaaa gtatggaaaa
    11701 aggtggatgg gatctcacaa aagttgatat ggaagctctt aaagaatcca agcttgctct
    11761 tgtattcggg cagatgaatg agcctcctgg cgcacgcgca agggttgccc tttctggcct
    11821 ttcaatagca gaacatttca gggacggcga tggaaaaagt ggcggaagag acatactctt
    11881 ctttatggat aacgtattcc gttttaccca ggccggttca gaagtatcag cattgctcgg
    11941 tagaatgcca tctgcagtag gttaccagcc aaccctcgcc accgaaatgg gtattatgca
    12001 ggaaagaatc acttcaacaa aacacggctc cattacttca gtacaggctg tttatgtacc
    12061 ggccgacgac cttaccgacc ctgcaccagc aacaaccttc gcacacctcg atgcaacaac
    12121 tgtactcagc agaaaaatat cagagcttgg tatctatccc gctgtcgatc cgctcgattc
    12181 aacatcccga attctgacac cggaaatcgt gggtgtcgaa cattacagat gcgcccagag
    12241 agtaaaggaa atacttcaga gatacaatga actgcaggat atcattgcaa ttcttggaat
    12301 ggatgaactt tctgaggaag ataaacttat tgttcacagg gcaagaaggg tacagagatt
    12361 tctttcacag ccattccatg tcgcatcaca gtttacagga attcctggag tgctggtttc
    12421 aattgaagat acaataaaag gcttcaatat gataatcgat ggaaaggttg accaatatcc
    12481 tgaagcagca tttatgctgg tgggtactat cgaggatgca atcgaaaaag gagaaaaact
    12541 tctggcacag tcaagataac catcaaaatg aagatcgaaa ttataacccc ggatgataaa
    12601 atttacgaag gagatataaa gtctgtacga gtgccaggta gcaagggctc tttccaggta
    12661 ctcaaagatc acgcaccaat tgtttctacc cttgaccctg gtatga
//
LOCUS       DAOSNB010000034        12515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5033_length_12515_cov_4.133467, whole genome shotgun sequence.
ACCESSION   DAOSNB010000034 DAOSNB010000000
VERSION     DAOSNB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5033_length_12515_cov_4.133467"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1577
                     /locus_tag="PLG42_02730"
     CDS             309..1577
                     /locus_tag="PLG42_02730"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOU30158.1"
                     /translation="MKIDILLGLQWGDEGKGKIVDALSPDYDIIARFQGGPNAGHSIV
                     FNNVKYILHLIPSGIFREGKLNLIGNGVVLDPAVFKQEVNSLGIPLEEIKKRVIISKR
                     AHLILPGHRILDAASEHVKGSSKIGSTLKGIGPAYTDKAARNGIRVGDIVRKDFKELY
                     KRVLEMHLRTAAGLGFEFNLSDYEEGWFEGVELLREFKLINSEYFLNEMIRNNKKILA
                     EGAQGTMLDIDFGTYPYVTSSNTISAGAFTGLGVSPARAGEVFGIFKAYCTRVGSGPF
                     PTELTDETGEEIRKRGVEFGATTGRPRRCGWLDLPALKYAIMINGVTRLFMTKADVLS
                     GLGSVRVCTSYKINGEESEEVPFEYNVCKTEPVYREFPGWQEDISNIKKYDDLPETLK
                     KYVEFIEKETGVPVTLISVGSDREQTVYKS"
     gene            complement(1597..2073)
                     /locus_tag="PLG42_02735"
     CDS             complement(1597..2073)
                     /locus_tag="PLG42_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30159.1"
                     /translation="MRKLYIILILVVLIPFTSCRWFKGKGIFGRKVDTMAVWQAKQDS
                     IRIADSLRAVQERIALENQRLEQERLAEQQRLEWERKYKYNIIVGSFITPEYARNCLA
                     DYQAKGYTNARIIPLENTRFEMVSAEAHDNFRTAVNRLKQFQDTVAFDAWLYIYSK"
     gene            2304..4217
                     /locus_tag="PLG42_02740"
     CDS             2304..4217
                     /locus_tag="PLG42_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOU30160.1"
                     /translation="MKSLVQFFEENVEKFSNNVYLWEKPGDKYEGMTYSEVRKHVHEF
                     AAGLINLGIKKGDRISLISEGRNSWVIGELGVLYTGAVNVPLSVKLNPEEIKFRLNHS
                     ESRMVMTSSIQYQKLKEIISDCPNIEKIIHFDAREEYPPNEIHFNEVKKIGREWLEKP
                     GNYEEFEKIYKSVTPNDFANICYTSGTTADPKGIILTHGNYVTNVYQAYSLMDIPPFF
                     KTLLMLPWDHSFGHTCGIYAFMGKGASIAAVKTGKTPMETLRNIPSCLKEIKPNLLLS
                     VPALSKNFRKNIEKAIREKGKFTEMLFNHALKIAYAYNKEGYNRGTGIQKIYKPLLSL
                     YDKILFSKIREFFGGELEYFVGGGALLDIELQRFFYALGVPVFQGYGLTEASPVISSN
                     SKERHKLGSSGVIVNNMDLKICDENGNELPVGEKGEIVIRGGNVMYGYWKNEKATKET
                     IRDGWLYTGDMGYMDKDGFLYVLGRYKSLLIADDGEKFSPEGIEEAISEQSKYIDQCM
                     LYNNQKPYTVALVVPNQHALKIYLEEKGLTADSDEGKRAVLTLIENEINEYRTTGKYG
                     NMFPQRWLPVAIGVLDEAWTEDNGLMNSTMKIVRGKITEKYADLIDYLYTPVAKVITH
                     ERNMEAVERMKLG"
     gene            4366..5826
                     /locus_tag="PLG42_02745"
     CDS             4366..5826
                     /locus_tag="PLG42_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOU30161.1"
                     /translation="MGELSGLRPRRVWGYFEEICRIPRISKHEEKIRKYLLEFASKNS
                     LQAFEDSGGNILIVKPPSPGFERKKTVILQSHMDMVGETLSGHRHHWNTDPVIPKICD
                     GWVKAENTTLGADDGIGMAAQLAVLTDENLKTGRIECLFTADEETGMTGAISLKPDFF
                     TGQILINLDSEDEGIIYIGCAGGLDTTAKMKFQTAPVPEGYVAYEISLTGLHGGHSGD
                     EIHKGYGNSVKIMSELLSNLTQKYQVGLHVFNGGNARNAIPREAVAVITSNPMYNGHI
                     KEEVNRFLSELAAKYGSLEPGLNLSLRNIPVPGAIMDSESHSKLLEALTRCPNGVIKW
                     SEEMEDLVETSTNLASVKFVDTNVVQIVTTQRSSVDQAKKEISENVKECFSSIGCEVH
                     SSDGYPGWKPNTSSEILGIARASYKILFGTEPQVRAIHAGLECGLIYEKNKNIDMISI
                     GPTIKGAHTPDEMLNIESVERFWQLLTEILRNIPEE"
     gene            complement(5850..6266)
                     /gene="mce"
                     /locus_tag="PLG42_02750"
     CDS             complement(5850..6266)
                     /gene="mce"
                     /locus_tag="PLG42_02750"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOU30162.1"
                     /translation="MKISHIEHIGIAVADLKASIKIYEEVFGLKCYKIEEVPGQKVRT
                     AFFKVGDTKIELLESTDPEGPVGKFIEKKGEGLHHIAFAVEDIKEGIADAISQGAQMI
                     NQEPVKGADDMLVAFLHPKSTRGVLIEICEKEKQVM"
     gene            complement(6313..7869)
                     /locus_tag="PLG42_02755"
     CDS             complement(6313..7869)
                     /locus_tag="PLG42_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU30163.1"
                     /translation="MRSSLLKKIRQAVALAVFIVFCVIFLDYRHLIPEKYFDIPLWFQ
                     FVPSAIKFVTIGMVAGSGFLAVLLLTILTGRTYCSFLCPLGILQDLFSRIGGKIKRRF
                     KRFGFKRPHTVLRYSLLAVTVLVVSFGGIYVLSLLDPYSIFGRFATYFAKPVILALNN
                     FLSGILEKFDVYTIAHVQIPTFNALVYLVPAGFLLLVGILSLTKGRLYCNSICPVGTF
                     LGLISKISLLRIKFDESKCTKCGRCAIACKSFCIDFLNKKIDNSRCVECFNCINSCPD
                     KALSYSLVTTGKKKTETDSEKRNFIGGLILILTGLSVKSLAQEEEQKVEHKASTVKEK
                     KTFPVSPPGSTGIENFNSKCTACSLCISACPNNVLVPSLVQYGITGIMQPHMDYHKGF
                     CAYECTRCLEICPTGAIMPLELEAKKLTKIGTAIFIRDNCIVETERTACGACAESCPT
                     KAVHMVPFEGNLTIPETNDEICIGCGHCEFACPVVPYKAIYVDGIPVHKAAKKPENVQ
                     TDLKHEDFPF"
     gene            complement(7875..8795)
                     /locus_tag="PLG42_02760"
     CDS             complement(7875..8795)
                     /locus_tag="PLG42_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU30164.1"
                     /translation="MKRRDFIIKSASAGFAASAALSLGGYKKLIDDKPLSQSYDLVAI
                     MGGKPEAMLDLGIQELGGMGKFVSRGQKVLVKPNIGWDRTPEYAACTNPALVKRIIEH
                     CFRAGAKEVYVFDHTVDNWVNCYRNSGIEKAAKDAGAKVVPANTENYYQEISIPEAKI
                     LKKTKVHQLLLETDVFINVPVLKNHDATHMTCAMKNMMGVVWDRAYWHRNNLHQCIAD
                     YALYNKKPVLHVIDCYNVMTKHGPQGVSKEDVAMLESQIITTDWVAGDAAAAKMIKLD
                     PLRIDYIKIAHDMGIGNMNLDSLNIRRIKM"
     gene            8979..10169
                     /gene="larC"
                     /locus_tag="PLG42_02765"
     CDS             8979..10169
                     /gene="larC"
                     /locus_tag="PLG42_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOU30165.1"
                     /translation="MKFLYYDCFSGISGDMNLGAMIDLGVDVKYLNDELGKLNLDGWK
                     LDVTRDQRHGICGTKVTVVQTKHEHAHRHLTDIYKIIDSSALDRKTKELSRKIFRKLA
                     EAEALVHGIPVEEVHFHEVGATDAIIDIVGAAICFNALNVDEVHVSVIETGGGTVRCE
                     HGVLPVPAPATAELLRNIPIRKGGVDFEATTPTGAAIINVLGKVFNKPVNMKILKTGH
                     GIGHKDHPEVPNILRVFLCEPEELTEEGHEAVLLECNIDDMNPEHYDYISEKLFSAGA
                     SDVFLSNIIMKKSRPGIILSVICEKNNVDALKYIIFTESTTLGIRCFSFQKETLSRTF
                     ETVQTLYGPVTFKRSFYKGKEVSFKPEYEECRKIAAETGKPLKEVYNNIISEIHNKRR
                     NESA"
     gene            10132..10950
                     /gene="larE"
                     /locus_tag="PLG42_02770"
     CDS             10132..10950
                     /gene="larE"
                     /locus_tag="PLG42_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOU30166.1"
                     /translation="MRYIIKEEMNLHDKLNHLDTILTGLNPLLIAFSGGVDSSFLVYR
                     ASGIKGLKMAAVTVKTIYIPDIELNEAVEFCRKHKIDHRILKMDVPDAVKNNPPDRCY
                     LCKKHIFSKILEFASLNNFKYLADGSNIEDMNSYRPGLKALSELSVRSPLAEANLSKE
                     EIRTALKEAGIEIWNKPSMTCLLTRLPYNTLITEKDIKMIEEAENVLFEKGFYGTRVR
                     KHGEIARIECLQGFMEKLINSSERNEIIQRLKELGFRYITVDLEGYRSGSMDNL"
     gene            10996..11745
                     /gene="larB"
                     /locus_tag="PLG42_02775"
     CDS             10996..11745
                     /gene="larB"
                     /locus_tag="PLG42_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOU30167.1"
                     /translation="MNISEIEKLLNDVKSGKKSVNEALEILKYFPYTDLGFAKLDHHR
                     EIRKGYPEIVYCSGKSVAQVKEIFRVLAEKETNVFGTRANEEMYEAVKSVVPEAVYYP
                     EARIISIKKKEPEIPETPIAVVTAGTSDIPIAEEAAVTAELLGNRVLRIYDAGVAGIH
                     RFLDRLNDLKKCRVIIVIAGMEGALASVVGGLVDKPIIAVPTSVGYGANFGGISALLA
                     MLTSCAAGVTVVNIDNGFGAAFSASMINKMK"
     gene            11742..12380
                     /locus_tag="PLG42_02780"
     CDS             11742..12380
                     /locus_tag="PLG42_02780"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOU30168.1"
                     /translation="MKRDRILLSTAYFPPIQYMALVNEAERVFIEREENYIKQTFRNR
                     CYILTANGVMALSVPVLEGSFHKRAIKDIRIDYTKRWQQIHLRSIYSSYKASPYFDYY
                     YEKVEKIISKSHTFLLDLNMESLQILVELMNIRPQITYTDFFEPVKTCEPDWRYFISP
                     KNREMDKLFRYKKYLQVFYDRFDFIPGLSSLDLIFNAGPDAVNYLPEILIPY"
BASE COUNT         4025 a         2373 c         2577 g         3540 t
ORIGIN      
        1 caaaaaatta atcgtggctt aaatgatttt acagaaaaaa agttgcacaa ctaatagcat
       61 tataaaaata taaattttat ttcttatttc aaatagtatt caacaatcaa tttaaaccag
      121 tatttaacaa ctaatttaaa ttagaagtct aatttgttga accattacgc tttgcgatat
      181 taaaataaat ccagctgttg cattacagca agacctattt ttcatttatt aacaacattt
      241 cagtaatcgg ttcttgattt aaatttattc gtaacttgtg aagcattttt taaacctaca
      301 gacttaaaat gaaaatagat attctacttg gtttgcaatg gggtgatgaa ggaaaaggaa
      361 aaattgtcga tgctttaagc cctgattatg acataattgc caggttccag ggcggtccga
      421 atgcagggca ttcaattgtg ttcaacaatg taaaatatat tttgcatttg attccatcgg
      481 gaattttccg ggaaggcaaa ttaaatctga taggaaatgg tgttgtactt gatcctgctg
      541 ttttcaaaca ggaagtgaac agtctgggta ttccccttga agagattaaa aaaagagtaa
      601 taatttcaaa gagagctcat cttatattac cgggtcacag aatacttgat gccgcatctg
      661 aacatgtaaa aggtagttca aaaataggtt ccacccttaa gggtataggt cctgcatata
      721 ccgacaaggc agcaagaaac ggaataaggg ttggtgatat agtgcggaag gattttaaag
      781 agctgtataa aagggttctt gaaatgcatc tgagaactgc ggccggtttg ggttttgaat
      841 ttaatctgag tgattacgag gagggatggt ttgaaggagt ggaactgctc agggaattta
      901 agcttatcaa cagtgagtac tttctgaatg aaatgatcag aaataataaa aaaattcttg
      961 ccgaaggtgc acagggaacg atgcttgaca tagattttgg gacatatcca tatgttacat
     1021 cctctaatac aataagcgca ggggcgttta ccggacttgg tgtatctcct gcaagagccg
     1081 gcgaggtatt tggaatcttt aaagcgtact gcaccagagt aggtagcggg cccttcccta
     1141 ctgaactgac tgatgaaaca ggagaagaaa tcaggaaaag aggagttgaa tttggtgcta
     1201 caacgggccg cccacgaaga tgtggctggc ttgaccttcc cgccctcaaa tatgcaataa
     1261 tgatcaatgg tgttaccaga ctctttatga caaaggccga tgtattatcc gggctcggat
     1321 ccgttagagt ttgtacttca tacaaaatta acggggaaga atctgaagaa gtgccttttg
     1381 aatataacgt ttgtaaaaca gaacctgttt accgcgaatt cccgggctgg caggaagata
     1441 tatccaacat aaaaaaatat gatgatctgc ctgaaactct caaaaaatat gtggagttta
     1501 ttgaaaaaga aacaggtgta cctgtaaccc tgatatcagt aggttccgac agggaacaga
     1561 cagtttataa atcctgatgc gggtaactgg taaaaattat ttgctgtaaa tataaagcca
     1621 tgcatcgaat gcaacggtat cctgaaactg cttcaaacgg tttacggcag tgcggaaatt
     1681 gtcatgcgcc tcagcagaaa ccatctcaaa ccttgtattt tcaagaggta taatcctggc
     1741 atttgtatat ccttttgcct ggtaatctgc cagacaattc cttgcatatt caggtgtaat
     1801 aaaactgcca acaataatat tgtacttgta ttttctttcc cactcaagcc tctgttgttc
     1861 ggcaagcctc tcctgctcca gtcgttgatt ttcaagtgct attctttcct gaaccgccct
     1921 caatgaatca gctatcctga tactgtcctg cttcgcctgc cacacagcca ttgtatcaac
     1981 ttttctgcca aatatgcctt tccctttaaa ccatcggcac gaggtgaagg gaataagaac
     2041 aaccagtatt aaaataatat ataactttct cataaataat gaattaattg aaatttaatt
     2101 gaactgattc aatgttaaaa caaatgtaca aataaatgac caatataaca aattgaattt
     2161 tcacaagtta tgaaccagtg ttgctcttaa aacagagata ttttaccctt taatcgtaaa
     2221 aaacaattta aatattccta atttcgtata ttagaacctg attttgctaa aagatttgta
     2281 atcttaactt aaaagcacat aaaatgaaat cgctggtaca attttttgaa gaaaatgttg
     2341 aaaaattcag taacaatgta tatctgtggg agaaacccgg agataaatat gaaggaatga
     2401 cctacagtga ggttagaaaa catgttcatg aatttgctgc cggactaata aacctcggta
     2461 taaagaaggg agaccggatc agtttaatat cagaaggtcg taatagctgg gtcataggtg
     2521 aattaggcgt tctttatacc ggagctgtaa acgtgcctct ttctgttaag ctcaatccgg
     2581 aggaaataaa gttcagactg aaccattcag aatcgaggat ggttatgacc tcatcaatac
     2641 agtatcagaa gctgaaagag ataattagcg attgccctaa cattgagaaa attattcatt
     2701 ttgatgcccg tgaagaatat ccgcctaatg aaatacattt taatgaagta aaaaaaattg
     2761 gcagggaatg gcttgagaaa cccggtaatt acgaggaatt tgaaaaaata tataaaagcg
     2821 ttacaccaaa tgattttgca aacatctgtt acacttccgg aacaactgcc gatccgaagg
     2881 gaataatcct cactcacggg aattatgtaa ctaatgtata tcaggcatat tcactgatgg
     2941 atattccacc ctttttcaaa acgctgctta tgcttccgtg ggatcattcc ttcgggcaca
     3001 cctgcggtat atatgctttt atgggcaaag gtgccagcat agcagctgta aagaccggaa
     3061 agactcctat ggagactctc cgcaatatac cctcatgtct aaaggaaatt aagcccaatt
     3121 tacttctgag cgttcctgct ctctcaaaaa acttcaggaa aaacatcgaa aaggcaatcc
     3181 gcgaaaaggg caaatttacc gaaatgctgt tcaatcatgc acttaaaatt gcatatgctt
     3241 ataacaaaga aggatataac aggggaactg gtattcagaa gatttataaa ccattgctct
     3301 ctctatacga taagattctc ttcagtaaaa tccgtgagtt tttcggtggg gaacttgaat
     3361 atttcgtcgg cggtggagct ttactcgaca ttgaacttca gagattcttc tatgctctcg
     3421 gcgtaccggt tttccagggc tatggcctta ctgaagcatc accagtcatc tcatcaaact
     3481 ccaaagaaag acacaaactt ggatcttcag gtgttattgt aaataacatg gacctgaaaa
     3541 tctgtgatga aaacggaaat gaacttccag ttggcgaaaa gggcgaaata gtaatcaggg
     3601 gcggcaacgt tatgtacggt tactggaaaa atgaaaaagc gacaaaagag acaataagag
     3661 acgggtggct ttataccgga gatatgggat atatggataa agatggtttc ctgtatgttc
     3721 tgggacgtta caagagcctg ctcatagccg acgacggtga aaaattcagt cccgaaggta
     3781 tcgaggaggc tataagtgag cagtcaaaat atattgacca gtgcatgctg tataacaatc
     3841 aaaagccata cacagttgcc cttgttgtcc cgaatcagca cgctctgaaa atctaccttg
     3901 aagaaaaagg acttacagcc gactcggatg aagggaagcg agccgtcctg acacttatag
     3961 aaaatgaaat taatgaatat cgcacaacag ggaaatatgg aaatatgttc ccacagagat
     4021 ggctaccggt ggcaataggt gtgcttgatg aggcatggac cgaagataat ggacttatga
     4081 actccacaat gaaaatagtc aggggaaaaa taaccgaaaa atatgccgat ctgattgatt
     4141 acctctacac acctgttgct aaagtcatta cccatgaaag aaacatggag gcagtggaaa
     4201 gaatgaaact aggctaaagt ggaacttctt acaatatgtc ttttttaatt atcgacacag
     4261 aaaaagtgta ttattaacat tttttatatt ttgccaacaa aaataatctt acctttgtgc
     4321 ttcacttaac aaattaagtt gctgaaaatt aatgatattg tttaaatggg agagttgagt
     4381 ggattgagac cccgcagggt ttggggatat tttgaggaga tttgcaggat accgcgcata
     4441 tcaaagcatg aagagaaaat aagaaaatac cttcttgaat ttgcatcgaa aaactctctg
     4501 caggcttttg aggattccgg cggaaatatt ttaatcgtaa aaccaccttc accgggcttt
     4561 gaaaggaaaa aaactgtaat ccttcagagc catatggaca tggttggtga gacattatcg
     4621 ggtcatcgtc atcactggaa cactgatcct gtaattccta aaatatgcga tgggtgggta
     4681 aaagctgaaa atacaaccct gggtgccgac gatggcatag ggatggcagc acaactggcc
     4741 gttctgacag atgaaaacct gaaaaccggc aggatagaat gcctctttac agcagatgaa
     4801 gagaccggca tgacaggagc aataagcctg aagcctgatt ttttcaccgg gcagattctg
     4861 atcaatctcg attcggaaga tgagggcatt atttatatag gctgtgctgg aggtctggac
     4921 acaactgcaa agatgaagtt ccagactgca ccggtgcctg aaggctatgt ggcatacgaa
     4981 atatcattaa caggcctgca tggcgggcat tcaggagacg aaattcacaa gggttatgga
     5041 aactccgtga aaataatgtc ggaactgctc tccaacctta cccaaaaata tcaggtaggg
     5101 ctacatgtct ttaacggcgg aaatgccaga aatgcaattc caagggaagc agttgctgtc
     5161 ataacctcaa acccaatgta taacgggcat atcaaagaag aagtaaacag atttctttca
     5221 gagcttgcag cgaaatacgg ttccctcgaa cccggactaa acctttcgtt aaggaatatt
     5281 cctgttccag gggcaataat ggattcggaa agccattcaa agctactgga agctcttacc
     5341 agatgcccta acggagtaat aaaatggtcg gaagagatgg aagacctggt tgaaacctca
     5401 acaaacctgg cttctgtaaa atttgtggat acaaatgttg ttcaaatagt tacaactcag
     5461 agaagttcag tggaccaggc aaagaaggaa atatcggaga atgtaaaaga atgtttttca
     5521 tcaatagggt gtgaagtaca ttcatccgat ggatatcctg gctggaagcc aaacacctca
     5581 tctgaaatac tgggtattgc aagagcttca tataaaatac tttttggcac agaaccacag
     5641 gtaagagcta ttcacgccgg actcgaatgt ggacttattt atgaaaagaa caaaaacatc
     5701 gacatgatat ctattggccc cactataaag ggggcacata cacccgatga gatgctgaac
     5761 atcgaatctg tagaaaggtt ctggcagctg cttaccgaga ttcttcggaa tattcccgaa
     5821 gagtaagatg agcaaaaaat cagtgatttt tacattactt gtttttcctt ttcacatatt
     5881 tcaatgagaa cacccctggt tgattttgga tgcagaaaag caacaagcat atcatccgca
     5941 cctttgacag gttcctggtt tatcatctgg gctccctgac tgattgcatc agctatccct
     6001 tccttaatat cctccactgc aaaagcaata tgatgcagcc cctccccttt tttctcaatg
     6061 aatttaccta caggtccctc agggtcggtt gactccagta gttctatctt tgtatcaccc
     6121 accttaaaaa aggcggtcct caccttctgc ccgggcacct cttcaatttt gtaacatttc
     6181 agcccgaaaa cctcttcata aatttttata gatgccttaa ggtcggcaac cgctatgcca
     6241 atgtgctcaa tatgtgaaat tttcatgtct tttctgttaa tctgttaaag ttcattgtct
     6301 ttcttcttga gttcagaaag gaaaatcctc atgcttcagg tcggtctgaa cattttcggg
     6361 tttctttgca gctttatgaa ccggaatgcc gtcgacatag attgctttat agggtacaac
     6421 ggggcaggca aattcacaat gaccgcatcc tatgcatatc tcgtcatttg tttcaggtat
     6481 ggtaagattt ccctcgaaag gcaccatatg gacagccttt gtcgggcagc tttcggcgca
     6541 ggctccgcat gcggtgcgtt cggtttcaac aatgcaatta tccctgataa atatcgccgt
     6601 tcctattttt gtaagtttct ttgcttccag ctcaagaggc ataattgcac cggtagggca
     6661 aatctcaagg catctcgtac actcgtaagc acagaaccct ttatgatagt ccatgtgtgg
     6721 ctgcataata cctgttatac cgtactggac aagcgaaggt accagcacat tgttcggaca
     6781 ggcagaaata cacagtgaac atgctgtaca ttttgaatta aaattctcga tgcctgtcga
     6841 gccaggaggt gataccggaa aggtcttttt ctcctttaca gttgatgcct tatgttcaac
     6901 cttctgttcc tcttcctgcg ccagcgactt aaccgatagc cccgtaagaa ttaatattaa
     6961 acctccgata aaatttctct tttcactatc agtctctgtc tttttcttcc ctgttgttac
     7021 cagactgtac gacaaagctt tatccgggca actatttatg caattgaaac attccacgca
     7081 acgtgaattg tctatttttt tgttcagaaa gtcaatacaa aacgacttgc atgcaatagc
     7141 acacctcccg cattttgtgc atttcgattc gtcgaatttt attcgcagta aagaaatttt
     7201 cgaaatcaaa ccaagaaaag tgccaacagg acagatagaa ttacagtaaa gccttccctt
     7261 tgtaagagac aatattccga ccagcaggag gaatcctgct ggaaccagat aaacaagcgc
     7321 attaaatgtg ggaatctgta cgtgcgctat tgtgtaaaca tcgaattttt cgagaattcc
     7381 cgatagaaaa ttattcagtg ccagtataac cggctttgca aaataagtag caaaacgtcc
     7441 gaaaatactg tatgggtcaa gaaggcttaa aacgtatata cctccaaaac tgaccaccag
     7501 cacagttact gcaagcaatg aatatcttaa aactgtgtga ggtcttttaa aaccaaagcg
     7561 tttaaaacgc ctttttatct tcccccctat tctgctgaac aggtcctgca atattccgag
     7621 aggacacagg aacgaacagt atgttcttcc ggttaaaata gtcaggagca aaactgcaag
     7681 aaaacctgat ccagcaacca tcccaatggt tacaaatttg attgcagatg gtacaaactg
     7741 aaaccagaga ggtatatcaa aatatttttc aggtatcaga tggcggtagt cgagaaatat
     7801 tacacaaaaa acaataaaaa cggcaagagc tacagcttgc cgtatttttt taagtaaaga
     7861 tgatctcatt cctattacat tttgattctt cggatattca gtgaatcaag attcatattc
     7921 ccaatgccca tatcatgggc aatctttata tagtctattc tcaacgggtc gagtttaatc
     7981 attttggctg ctgcggcatc accagctacc cagtcggttg ttattatctg cgattcaagc
     8041 attgcaacat cttctttcga aactccctgt ggcccatgct tggtcataac attataacaa
     8101 tcaataacat gcagaacggg ctttttatta tacaatgcgt aatcggcaat acactgatgc
     8161 aggttgtttc tatgccagta tgccctgtcc catacaacac ccatcatatt tttcattgca
     8221 caggtcatat gagttgcatc atgattttta aggacaggga cattaataaa tacatccgtt
     8281 tcgagtaata attgatgaac tttggttttt ttgagaattt ttgcttcagg aatagatatt
     8341 tcctggtaat aattctcggt gttggcaggt acaactttgg ctcctgcatc ttttgcagct
     8401 ttttcgatgc ctgaatttct gtaacaattc acccagttat cgacggtatg gtcaaaaaca
     8461 taaacttcct ttgcacctgc cctgaaacaa tgctcaataa ttctttttac gagtgcaggg
     8521 tttgtacatg ctgcatattc cggggttcta tcccacccta tattaggttt tacaagaact
     8581 ttctgccctc tggaaacaaa cttgcccatt cccccaagtt cctgaatgcc aagatccaac
     8641 attgcttcag gtttacctcc cattattgct acgagatcgt acgactgact taagggtttg
     8701 tcgtcgatga gtttcttata gccgcctaag ctcagggcag cgcttgctgc aaaacctgcg
     8761 cttgccgatt tgattataaa atctctgcgc ttcattatga gttttttatt attgattcaa
     8821 taattgtgcg aaattacatt tttttctcta ttgaataatc acatatttta acataaggaa
     8881 acacaagcag actatttaac atattttaat tgttccaaac tattaacttt acgggcgata
     8941 aattgttatt agtaacataa acttaacaat cacctgcaat gaagtttctc tattacgatt
     9001 gtttttcagg aatcagtggg gatatgaacc tgggtgcaat gattgatctg ggtgttgatg
     9061 tgaaatatct gaatgatgag cttggaaaac ttaaccttga cggatggaaa cttgatgtaa
     9121 ccagggatca gcggcatggg atatgcggaa ctaaggttac tgtagttcag acaaagcatg
     9181 aacacgctca cagacatctc acggacattt ataaaattat tgactcatcg gctcttgaca
     9241 gaaagacaaa agaactgagc cggaagatat tcagaaagct tgctgaagct gaagcattgg
     9301 tgcacggaat accggttgag gaggttcatt ttcatgaagt tggtgcaact gatgctataa
     9361 ttgacatagt aggcgcagca atttgtttta atgctctgaa tgttgatgaa gtacacgtat
     9421 cggtaataga aacgggtggg ggaactgtaa gatgtgaaca tggtgtattg cctgtgcctg
     9481 ctcctgctac agcagaattg ctcaggaata ttccaataag aaaaggagga gttgattttg
     9541 aagcaacaac accaacaggc gcagcaataa ttaatgttct gggaaaagtt tttaataagc
     9601 ctgttaacat gaaaattctt aaaacaggcc acggaatcgg acataaagat catcctgaag
     9661 tgccaaatat tcttagagtt ttcctttgtg aaccagagga attaacagag gaaggtcatg
     9721 aggcagtttt gctggaatgt aatattgacg atatgaatcc ggagcactac gattacattt
     9781 ccgaaaaact ttttagtgca ggagcgtcag atgtattcct ctcaaacatt attatgaaga
     9841 aaagcagacc aggtatcata ctcagtgtta tatgcgaaaa aaataatgta gatgctctga
     9901 agtatattat ctttacagaa tcaaccactc tgggtatcag gtgcttttcc tttcaaaaag
     9961 agacactatc aagaactttt gaaactgttc agaccctgta cgggcctgta acctttaaaa
    10021 ggtcattcta taaaggcaaa gaagtatctt ttaaacccga atatgaagag tgccgcaaaa
    10081 ttgccgcaga gaccggaaaa cctctgaaag aagtttacaa caacatcatc agtgagatac
    10141 ataataaaag aagaaatgaa tctgcatgat aaattaaatc atctcgacac aattcttacc
    10201 ggactgaacc ctttattgat tgccttttca ggtggtgtag acagttcttt ccttgtgtac
    10261 agggcatcgg gaataaaagg gcttaaaatg gctgctgtaa ctgtaaaaac gatatatata
    10321 cccgacattg aattaaatga agctgtggag ttctgcagaa aacataagat tgaccacaga
    10381 attcttaaaa tggatgtccc tgatgctgta aaaaacaatc cacctgacag gtgttacctg
    10441 tgtaaaaaac acattttcag taaaattctt gagtttgctt cacttaacaa tttcaaatac
    10501 ctggctgatg ggtcaaacat tgaggatatg aacagctatc gcccgggtct taaagctctt
    10561 tctgagcttt cagtaagaag cccgctggca gaggcaaacc tttcaaaaga agagatcaga
    10621 acagcgctga aagaagcagg aatcgaaata tggaacaaac cgtcaatgac gtgtctactt
    10681 acaagattac cttacaatac tttaattaca gaaaaagata tcaaaatgat agaggaagcg
    10741 gaaaatgtcc tgttcgaaaa aggattttat ggcacaagag taaggaaaca tggtgagatt
    10801 gcacgtatag aatgtttaca gggctttatg gaaaagttga ttaattcctc cgaaagaaac
    10861 gaaattattc aaagattaaa agaattaggt tttagataca taactgttga tctggagggt
    10921 tacaggagcg gaagcatgga taatttgtga gagataaatt aatcatactt gtaatttgta
    10981 gtaattgaat aaattatgaa tataagcgaa attgaaaaac ttctgaatga tgtaaagtca
    11041 gggaaaaaga gtgtaaatga agcacttgag atattaaagt atttccctta caccgacctt
    11101 ggttttgcaa aactcgacca tcacagggag ataagaaaag gttatcccga gattgtttac
    11161 tgttcaggta agagtgttgc acaggttaag gagattttca gggtgctggc tgaaaaagaa
    11221 acaaatgtat tcgggacaag ggcaaatgaa gagatgtatg aggctgttaa aagtgttgta
    11281 cccgaagctg tatattaccc tgaagcaagg ataataagca taaaaaagaa ggagcctgaa
    11341 atacctgaga cgccaatagc tgttgtgacg gcaggaacgt ccgacatacc tatagctgag
    11401 gaagctgctg taactgctga attgctgggc aacagggttt taagaattta tgacgctggc
    11461 gttgcgggta ttcatcgttt ccttgacagg ctcaatgatc ttaaaaagtg tcgtgtaatt
    11521 attgttattg caggaatgga aggagcgctg gcaagtgtag ttggagggct tgttgataag
    11581 ccgataattg ctgttccaac aagcgtgggt tatggtgcca acttcggggg aatttcagct
    11641 ctgctggcta tgcttaccag ctgtgctgca ggagtaactg tggtcaacat cgacaacggc
    11701 ttcggagcag ctttctcagc aagcatgatt aataaaatga aatgaaaagg gacagaatcc
    11761 ttctttcaac ggcctatttt cctcccatac aatacatggc actggtaaat gaggccgaaa
    11821 gagtattcat agaacgggaa gaaaattaca taaaacaaac attcagaaac aggtgctaca
    11881 ttctcacagc taatggggtt atggctcttt cagtacctgt tcttgaaggc agttttcata
    11941 aaagagcaat aaaagatatc aggattgatt ataccaaaag atggcagcag attcatctgc
    12001 gaagtatata ttcttcttac aaagcatctc catatttcga ctattactat gaaaaagtcg
    12061 aaaaaataat ctcaaaaagc catacatttt tacttgatct caatatggaa tcgcttcaga
    12121 ttctggttga gttaatgaat atcagaccac aaataacata caccgatttt tttgaacctg
    12181 taaagacctg cgaacctgat tggagatatt ttataagccc taaaaacagg gagatggaca
    12241 aattattcag gtataaaaaa tatctccagg tattttacga ccgctttgat ttcattccgg
    12301 gattaagctc tcttgacctg attttcaatg caggcccgga tgccgtgaat tatcttcctg
    12361 agatattaat cccttattga atatttctga ttccttgaga acgccctatt gttaaaaggc
    12421 tttttatttc ccaatatttc tttcagaatt taaatccaag ggttgcagta aagttatttc
    12481 tggtatatgt aatatcagcc ggtttaaggt aatca
//
LOCUS       DAOSNB010000035        12375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5103_length_12375_cov_3.940341, whole genome shotgun sequence.
ACCESSION   DAOSNB010000035 DAOSNB010000000
VERSION     DAOSNB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5103_length_12375_cov_3.940341"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLG42_02785"
     CDS             <1..297
                     /locus_tag="PLG42_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU30169.1"
                     /translation="LAILNRRNKNGTASLDDLAGILARSVEGSTWFDGDEAQRKLQNA
                     ILKLPEKQRVVFNLRYYDEMPYEDMSKVLNTSVGALKASYHHAVKKIEEYLQTL"
     gene            332..757
                     /locus_tag="PLG42_02790"
     CDS             332..757
                     /locus_tag="PLG42_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30170.1"
                     /translation="MKNLDKIPKQNPFRVPDNYFEDVSKKIIAATSEAGGKQKHPGKV
                     IRIRYIIAIAASVAALAIIGITGLLTNTRNGNLDIASINLNSEQVLETIIYDIDILTL
                     EQEISDSWFETGLTGLDSEEIIDYLINENIEPSEIYELL"
     gene            770..1252
                     /locus_tag="PLG42_02795"
     CDS             770..1252
                     /locus_tag="PLG42_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30171.1"
                     /translation="MKKGLIITVLLLFAAFQFASAQQDAPGDRLYAYKVAFLTKRLNL
                     TPEEAQRFWPVYNELQVKKNEIQKERTSIIRRAAQNELNMSEKELTEAADRLVSLELQ
                     DANLTAEYHKKFREILSPVKIIRLYQAENQYRLELLRELRNAPAAPAEQVPQRRLRNN
                     "
     gene            complement(1260..2630)
                     /gene="hisS"
                     /locus_tag="PLG42_02800"
     CDS             complement(1260..2630)
                     /gene="hisS"
                     /locus_tag="PLG42_02800"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOU30172.1"
                     /translation="MALKPSIPKGTRDFSPAEVLKRDYIFSTIKDVFKLYGFQPLETP
                     AMENLSTLLGKYGTEGDKLLFRILNSGDFLEGVSESELQNPDHSVLSSKICEKGLRYD
                     LTVPFARYVVQHRNEITFPFKRYQIQPVWRADKPQKGRYREFYQCDADIIGSDSLLNE
                     YELIRIIDDVFSKLKIPVVVRLNNRKILMGIVEAAGVSGRFSEITVAIDKLDKVGMQA
                     VEEELVGKGIPRSAVESLKNILEVEGSNAGRMEKLKQFLVNSETGKKGIEEMETLFRL
                     INSDPPRSEIVFDLTLARGLDYYTGTIIEVKATDVSIGSICGGGRYDNLTGIFGLEGV
                     SGVGISFGADRIYDVLSLKEGFSSLGTSASQVLIVNFGDTELQYALRILKILHSHGIS
                     AQIYPDAIKIGKQFSYANSLNIPYVVVAGEDEIKGSEVTVKNMAEGSQTRISIEKLGD
                     FLTIQT"
     gene            complement(2722..3624)
                     /gene="ppk2"
                     /locus_tag="PLG42_02805"
     CDS             complement(2722..3624)
                     /gene="ppk2"
                     /locus_tag="PLG42_02805"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOU30173.1"
                     /translation="MASKKKTREETEKNKKEEKQKVDKVEVFDKDELYHDEEVKIKKK
                     MKKKVYFKELRKLEIELIKLQEWVKAKKLKVVVIFEGRDAAGKGGVIKTIAGCLNPRI
                     CRVVALGVPTEKEKTQWYFQRYVAHLPAGGEIVLFDRSWYNRAGVEKVMGFCTNEEYE
                     EFLRSCPEFEKMLIRSGIILIKYWFSVSDHEQEKRFQDRIKDPTKRWKISPMDIESRD
                     KWVEYSMAKDKMFAYTDTKQSPWYVVEGDDKRRARLNCIHHLLSLIPYEDLTPKPFKL
                     PPLKHDVAYVRPPKTEQTYVPEVY"
     gene            complement(3835..4191)
                     /locus_tag="PLG42_02810"
     CDS             complement(3835..4191)
                     /locus_tag="PLG42_02810"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOU30174.1"
                     /translation="MKEIRVLSESERTWAMFCHLSAFACFIFPFGNILGPLICWLSRR
                     DDSTWVNENGKASLNFQLSLLLYTLLLIPLCFILVGIPLLIALWILNIVCVTIASIKA
                     SRGEEFRYPLAIPFIQ"
     gene            complement(4238..5089)
                     /locus_tag="PLG42_02815"
     CDS             complement(4238..5089)
                     /locus_tag="PLG42_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30175.1"
                     /translation="MKRLLLLSVFCSAVLLSRAQAPFPSAEEIKQFMNSKTCVVLEDD
                     PFSAWNSFIKKAVTSFWTITPYEFINIVEFNKRRTDPAYSFIVLTQTHFEKDKTGGLF
                     NFINLIQGKRVRDLSDNPEICAVPLSFADEDDTGYGYKLGIILAFIQKHAKLISEDPS
                     ITGRRYLRYYNVNAPEVVNKTILAKKEDLAPDINTIEAIKAIYPFPFKIVEEEEIVKA
                     INEKAPATLILHKVGPSDNRTGSFCFKILIGTDDSNMYYFGQHRINNSNPNAFLESDL
                     RRLARYK"
     gene            complement(5099..5860)
                     /locus_tag="PLG42_02820"
     CDS             complement(5099..5860)
                     /locus_tag="PLG42_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30176.1"
                     /translation="MKKTSKISWNALLYFIISSFIIPSAVAQETSEEKVKYTSDFRFK
                     DGIYLNFEQVKANSPIPKAKILTSADYNDRDFFNKVFASEKIYFYDEMGVRQEVKKED
                     IWGFARNGILYVMVQGNFNRITFFGNICHFVADITTYDNRYYSPYGYYDPYYYPYSYY
                     PYYYPYGGYPYGYYPPYYYRPYTASRTELRQYLIDFETGKIYEFDVESVKVLIMRDPE
                     LYEEFVKLTKKKQKELMFVYIRKFNERNPLYLPAK"
     gene            complement(5921..6601)
                     /locus_tag="PLG42_02825"
     CDS             complement(5921..6601)
                     /locus_tag="PLG42_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30177.1"
                     /translation="MRSGKTIPLVVFAILFPLSLEAQFTESRIILPFRFKDGIYKNFD
                     QVLRNEPVPKASLLIAEDFTDAEFYRRILKSKQIIYSTAEGKQEYIDNRKVWGFASDG
                     ILYVHWKGYFSPLYMLGTISCFFLHDSQFEIYKSIISENYGNEMELTAGDGKLVVRYR
                     DLKKYLLDFRTGKIMKLNVRNLSRLLKNEPEIFSEYRQLPQYRKRSRIFLYIRNYNEK
                     NPLSLPLN"
     gene            6790..8079
                     /locus_tag="PLG42_02830"
     CDS             6790..8079
                     /locus_tag="PLG42_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU30178.1"
                     /translation="MLSESKRYIIKNLFTIILLLLAASIILIFLRLVLAAGVTLIAVC
                     TLEILLLRQSLRQTRALENTIEEISELNEKTGDVISRFSHHIREPLNNLVVVGEMLAS
                     SELSKKQKDLVETLIASTNNMADILNDLTVQSAVAGYEERKQIRFNIGAAIENTIDLF
                     RLKSPQNIDFILHKKDAANFEVTGDPIILKQIFLDIFNIISEQSSEFLTKVTTSIKKE
                     KEKDRECIVSFRIQTDRRIIFLDDRNATSNHMIKLISSGGGNYAQELSENYSVFTFTL
                     KFVSIAEEKKKPIASPAIEALRVKEKHKKELKDANILLVEDNPINQKITLLTLKPLVN
                     SIDTASDGREALEKFGSSSYDLILMDIQMPVMSGIIAAEKIRALEASTSRHTPIIAIT
                     ANAMLGDKERCLAAGMDDYISKPFQPSSLIDIIKKHL"
     gene            complement(8090..8926)
                     /locus_tag="PLG42_02835"
     CDS             complement(8090..8926)
                     /locus_tag="PLG42_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU30179.1"
                     /translation="MIRNRKTLTSTISELNETKENLSILEYKLRNVTDSLVYARHIQK
                     ALLPSESFLANHFNDYFIFYQPRDVVSGDFYWFGITGDRICVISADCTGHGVPGALMS
                     IIGQNILNQVISNGNSDNPAEIMERLDVSVRNYFSREGEYYIRDSMDIGICMIDHKRK
                     VIEFAGAFSSMYFIKDNRLKEIKGDKQILGMKPEKFRFTSQVENFSGGEVVYLFTDGY
                     ADQFGGEEGKKFMYRRFRYLLTTINMLPLNEQKTILEETLNSWKGNNEQVDDILVIGL
                     RL"
     gene            complement(9158..11050)
                     /locus_tag="PLG42_02840"
     CDS             complement(9158..11050)
                     /locus_tag="PLG42_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOU30180.1"
                     /translation="MTSKGLAGFIHVLEREGELKRISVFVDPVLEIAEFADRAINSGG
                     PALLFENNGTAFPVLINAFGSEKRMNLALKTEEPENIYSEIDKISDLLAPGRSNPLNK
                     ISDFFTLLRLRKFIPVRTCRKGRCQQVILKDPDLEILPVLKCWPHDGGRFITLPIVHT
                     YNPFTLAPNAGMYRMQILDKCTTAMHWQLHKTGANHYRLWKETGKMMPVSVVLGGDPV
                     YTYAATAPLPENIDEYILAGFLTQRRIKMVKCVTNDIYVPEDADIVIEGYVDPSEPLV
                     SEGPFGDHTGFYSLSDMYPKFHVTCITHARDAVYPATVVGIPPKEDLFFTRATEKIFL
                     KPLQMVLNSEITDLHMPPAGVAHNLVIVSMKKNYPGQGLKVINSLLGAGQMMFSKYII
                     AVSDGIDIRNYSEVLEEVINNTDVKKDVIFSRGPLDVLDHSCDVPAFGGKAGIDATRK
                     LLSETLPGNKNRTDEKKITDVLNEMVKHQMISSYHILRNRSLYPVAVISVKREGKLVN
                     IEKIVQFLNNTGAGDVLKVVLAVDEEVDTGDLNMVAWQLLGNSDPLRDHRYFGTSSIL
                     FDGTVKAFRQGGFPRPWPDIVCSDEGTITSVDEKWSVAGMGNLVNSPSLKYLKLRKGN
                     SATVMP"
     gene            11195..11365
                     /locus_tag="PLG42_02845"
     CDS             11195..11365
                     /locus_tag="PLG42_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30181.1"
                     /translation="MKVGIKTVMGTILSISSHSPFAAEATYGGRLQLFWPAAFQVLRS
                     EGGNPELSGTAF"
     gene            complement(11397..11645)
                     /locus_tag="PLG42_02850"
     CDS             complement(11397..11645)
                     /locus_tag="PLG42_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30182.1"
                     /translation="MTLKGSVRPPGLRIREPKPELRNPAKEFPMFSRSLKTPTLFQRL
                     GHRAAGDKGLRSDETCKLNNPTGLISACLNDASSRNPE"
     gene            complement(11844..12365)
                     /gene="rplQ"
                     /locus_tag="PLG42_02855"
     CDS             complement(11844..12365)
                     /gene="rplQ"
                     /locus_tag="PLG42_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOU30183.1"
                     /translation="MLANMAVSLILNKRITTTTAKAKALRSYIEPLITRSKEDTTHSR
                     RMVFRYLQDKKAVSELFREISPKVADRPGGYTRILKTGNRLGDNAEMCIIELVDFNEA
                     RLAAQTKPEKTTTRRRRAPKKKAAGTAETAAAATGTAPQDNATKDVTTDTAEPDQEKS
                     SGSATEEDANKAE"
BASE COUNT         3667 a         2589 c         2301 g         3818 t
ORIGIN      
        1 cttgcaatat taaacagaag aaataaaaac ggtaccgcat cgctcgatga ccttgccggc
       61 atccttgcaa gatccgttga gggcagtacc tggttcgacg gcgatgaagc ccagaggaaa
      121 ctgcagaatg ctattctaaa gcttcctgaa aaacagaggg tggttttcaa tctgagatat
      181 tatgacgaaa tgccttacga ggatatgagt aaagttctta atacctcagt aggcgctctg
      241 aaagcttctt atcatcatgc agtaaaaaag attgaagaat atctgcaaac actttaaact
      301 ttaatgattt tgaaccatct aaaagacaac aatgaaaaat ctcgacaaaa taccgaaaca
      361 gaatcccttc agggtgcctg ataactattt cgaggatgtc agtaaaaaaa taattgcggc
      421 aacttcggaa gccggaggaa aacagaaaca tcccggaaaa gtaatcagga taaggtatat
      481 aattgcaatt gcagcttcgg ttgccgcact tgcaatcata ggtataaccg gactgctcac
      541 caacaccaga aacggtaacc tggatatcgc ttccatcaac ctgaattctg aacaggttct
      601 tgaaacaata atttacgata ttgacatttt aaccctcgaa caggaaattt ctgattcatg
      661 gtttgaaacc ggactgacag ggttagacag tgaagaaata attgattatc ttatcaatga
      721 aaatattgaa ccatctgaaa tttatgaact tttataaaaa atacagggta tgaaaaaggg
      781 acttataata actgttctgc tcctgttcgc agcgtttcag ttcgcctctg cacagcagga
      841 tgcacctgga gacaggcttt acgcttacaa ggtagcattt ttgacaaaaa ggctaaatct
      901 tacacctgag gaagcacagc gcttctggcc tgtatacaat gagcttcagg tgaaaaagaa
      961 tgaaattcag aaagagcgaa cttcaattat cagaagagct gctcagaatg aactcaacat
     1021 gtcagaaaaa gagcttacgg aagctgccga ccgtcttgtt tctcttgaac tgcaggatgc
     1081 aaaccttact gccgaatatc ataaaaaatt tagagagata ctatcacctg taaagatcat
     1141 aaggttatat caggctgaaa atcagtaccg gcttgaactt ctgagggaat taagaaatgc
     1201 acctgctgca ccggctgaac aggttccgca acgccgactg agaaataact gacttactgt
     1261 caggtttgaa tggttaaaaa atcaccaagt ttctcgatac tgattcttgt ctgggagcct
     1321 tctgccatgt ttttcactgt aacttcactt ccctttattt catcctcacc cgcaacaact
     1381 acataaggaa tatttaacga attggcataa gaaaactgct tccctatttt tattgcatca
     1441 gggtaaatct gggcgctaat cccgtgactg tggagaattt tcagtatcct gagtgcatac
     1501 tgcagttctg tatcaccgaa atttacaatg agtacctgcg atgcagatgt tcctaatgat
     1561 gaaaaacctt ctttaaggga aagtacatcg taaattctgt cagctccaaa agatatcccc
     1621 acacctgaga ctccttccag gccaaaaatt ccagttaaat tatcataacg gccaccaccg
     1681 caaatactgc caatgctaac atcagttgcc tttacttcaa ttattgttcc ggtgtagtaa
     1741 tcaagccccc ttgccagtgt aaggtcaaaa actatctctg aacgtggagg gtcggaattt
     1801 ataagccgga aaagggtctc catctcttca atacccttct tccctgtttc tgaattaacc
     1861 agaaactgtt tcagcttttc catccttccg gcatttgagc cttcaacttc aagaatgttt
     1921 ttcagactct caacagcact gcgcgggatc cctttcccaa ccagctcttc ctcaactgct
     1981 tgcattccca ccttgtcaag tttgtcgata gctacagtta tttcactgaa cctccctgac
     2041 acacctgctg cttcaacaat tcccattaaa atcttgcggt tattaagacg tacaacaacc
     2101 ggtattttga gctttgaaaa aacatcatca attatcctga taagctcata ttcatttaaa
     2161 agtgagtcac tgccaataat atccgcatca cactggtaaa actccctgta tctgcctttc
     2221 tgaggtttgt cggctctcca tacaggttgt atctgatagc gcttaaaggg gaaggtaatc
     2281 tcattcctgt gctgcaccac atatcttgcg aatggtactg taaggtcgta ccttaaaccc
     2341 ttttcgcata tttttgatga aagaacggaa tggtcgggat tctgaagttc actttctgaa
     2401 acaccttcaa gaaaatctcc cgaattgagt attctgaaaa gaagtttatc gccttctgta
     2461 ccatatttcc ctagcagtgt agagaggttt tccattgctg gtgtttcaag tggctgaaaa
     2521 ccatacagtt taaaaacatc ctttattgtg ctgaatatat agtccctttt taaaacctct
     2581 gccggtgaaa agtcgcgggt gccttttggt attgacggtt taagagccat gataatgcat
     2641 aatgtaacgg gcaaaagtat acatatactt tgaaagtaaa aagtaaatat taagacaacg
     2701 cggtaattta aatgtcaaaa ttcaatatac ctcaggcacg tatgtctgtt ctgtttttgg
     2761 tggtctcaca taggcaacgt cgtgttttaa gggaggcagt ttgaatggtt ttggtgtcag
     2821 atcctcataa gggattaatg aaagcaggtg gtgaatgcag ttaagccttg ctctcctttt
     2881 atcatctccc tcgaccacat accatggaga ttgtttcgta tcggtataag cgaacatttt
     2941 atcttttgcc atggaatatt ccacccattt atcacgcgat tcaatatcca ttggacttat
     3001 cttccatcgt tttgtcgggt cttttattct gtcctgaaat cttttctcct gttcatggtc
     3061 gcttactgag aaccagtatt ttattaatat tatgcctgaa cggataagca ttttttcaaa
     3121 ttccggacac gatctcagaa attcctcata ttcttcgttt gtacagaatc ccattacttt
     3181 ttccactcct gccctgttat accagcttct gtcgaagaga acaatctcac ctcctgccgg
     3241 aagatgagca acatatctct gaaaatacca ctgtgttttc tccttttctg tgggaactcc
     3301 aagagcgact accctgcaga tgcgaggatt tagacacccg gctatggttt ttattactcc
     3361 tcccttgcct gcagcatccc tgccctcaaa tattactaca actttcagct ttttagcctt
     3421 aacccactcc tgaagtttaa taagctctat ttcaagtttt ctgagctctt tgaaatagac
     3481 cttcttcttc atctttttct ttatcttcac ttcctcatca tgatagagct catccttatc
     3541 aaacacttca actttgtcaa ctttctgctt ttcttccttt ttgttctttt ccgtttcttc
     3601 cctggttttc ttttttgaag ccatggcatt aatgttttaa taacagaaag ttagacaatt
     3661 tttttaatac atactacctc aggtaattaa aatttttagc aatatgagaa aagaatatct
     3721 tgccaatagc tagaatattg ataatgaata aaatattaag acattagctc tctgcgcgac
     3781 taaaaaccag ggctactttg ctattctcag taagaaaatt actgaaatga tctgttactg
     3841 aataaacgga attgccagcg gatatctgaa ctcttcgcct cttgatgcct tgattgatgc
     3901 aatagtaacg catactatat ttagtatcca gagggcaatc agaagcggaa ttcctacaag
     3961 gataaaacac aatggtatca gcagcagtgt gtacagcaac aacgagagct gaaaattcag
     4021 tgaagctttt ccgttttcgt tcacccatgt agaatcatca cgtcgtgaaa gccagcaaat
     4081 aagaggaccc agaatattcc cgaaaggaaa aataaaacag gcaaatgcag acaggtggca
     4141 gaacattgcc caggtgcgct cgctttccga taaaactctt atctctttca tggtctttct
     4201 ttttaactaa agacggaaat aatcttactt tgctgcatca tttatacctg gcaagacgac
     4261 gcagatccga ttcgaggaaa gcattggggt tactattatt tatcctgtgc tgcccgaaat
     4321 agtacatatt tgaatcgtcg gtgccaatca gtattttaaa gcaaaagctc cctgtacgat
     4381 tgtctgaagg tcccaccttg tggagaataa gggttgcagg tgccttttca tttattgcct
     4441 ttacgatttc ttcttcttca acaatcttaa aggggaaggg gtatattgct tttatcgcct
     4501 caattgtatt tatatctgga gccagatcct ctttctttgc cagaatggtt ttattgacaa
     4561 cttcaggggc atttacattg taatacctca gatatcttct tccggtgatt gatggatctt
     4621 ccgaaataag ctttgcatgt ttctgaataa aagccagtat aatacctagc ttatagccat
     4681 agcctgtatc atcttcatct gcaaatgaga gaggaacagc acatatctcg ggattgtcac
     4741 tcagatccct cacccttttc ccctgtataa gattgatgaa attaaacaat ccgcctgtct
     4801 tgtctttctc gaaatgagtt tgagtaagta ctataaaaga gtatgcaggg tcggtgcgcc
     4861 ttttgttgaa ttcaacaata tttatgaatt catacggagt aattgtccag aaagaagtaa
     4921 cagccttttt aataaaagaa ttccatgccg aaaagggatc atcttccaga acgacacagg
     4981 ttttggaatt catgaactgc ttgatctctt cagcgcttgg aaacggagcc tgagccctgg
     5041 acagtagaac cgccgagcaa aaaactgaaa gaagcaataa tcttttcatg gaatctctct
     5101 attttgcagg taaatataaa ggatttctct cgttaaactt cctgatataa acaaacataa
     5161 gttccttctg ttttttcttt gtcagtttta cgaattcttc atatagttct ggatctctca
     5221 ttatcagaac tttaacgctt tccacatcaa actcatatat cttaccagtt tcaaagtcaa
     5281 tcagatactg cctcaactct gtacgactcg ctgtatatgg acggtagtaa tatggcgggt
     5341 aataaccgta aggatatcca ccatatggat aataatacgg gtagtatgag taaggataat
     5401 aatatggatc gtaatagcca taaggtgagt aatatctgtt gtcgtaagtg gttatgtctg
     5461 ccacaaagtg gcatatattt ccgaagaaag taattctgtt aaaatttccc tgaaccatta
     5521 cataaaggat accgttacgc gcaaatcccc atatatcttc tttcttaact tcctgcctta
     5581 cacccatctc atcgtagaag taaattttct ctgaggcaaa aaccttattg aaaaagtccc
     5641 tgtcgttata atcggctgat gtaagaattt ttgctttcgg aatgggactg tttgctttaa
     5701 cctgttcaaa attaagataa atgccatctt taaaacgaaa atcagatgta tatttaactt
     5761 tctcttctga agtctcctgt gcaacagcag atggtattat gaaagaagat ataatgaaat
     5821 agagcaaagc attccatgat attttactgg ttttcttcat attcctgccc tcctttcctc
     5881 attcatatat caaaaattat accgcatata ttgaaatatt ttaattcaaa ggtaaagata
     5941 aagggttttt ttcattataa tttctgatgt ataaaaagat cctggatctc tttctgtact
     6001 gtggaagctg ccggtattcg gaaaagattt ccggttcatt tttcaataac ctgctgagat
     6061 tcctgacatt cagcttcatg atttttcctg tcctgaaatc aagaagatat tttttcaaat
     6121 ccctgtatct taccaccagc ttcccatccc ccgcagttaa ctccatttca tttccataat
     6181 tttctgaaat aatactctta taaatttcaa actgactgtc atgcaaaaaa aagcaactaa
     6241 tggtacccag catataaagc ggactgaaat accctttcca gtgtacatat agaatcccat
     6301 ccgaagcaaa tccccatact tttctattat caatatactc ttgctttcct tcagcagttg
     6361 aatatatgat ctgttttgat tttaaaattc ttctgtaaaa ttcagcatca gtaaaatctt
     6421 ctgcaataag aagactggcc ttggggacag gctcattcct gagaacctga tcaaaatttt
     6481 tgtatatacc gtccttaaac ctgaaaggaa gtattattct ggattcagta aactgagctt
     6541 ccagcgacag tggaaacagt atcgcgaaaa caactaacgg tattgttttt cctgacctca
     6601 actcaacttc ctataaacaa tttgaatctt gaattaatcc aaaattagaa tgtttttaac
     6661 atatattatt ttttcatcgt ttttagctga gataataaaa atatcataat tttacgcatg
     6721 ataatgagac ctgttatgtt taaaatcagg aagaaaggcg aaagtggaca cataattcgg
     6781 ggattaaaga tgctctcaga atcaaaaaga tacattatta agaatctttt tactataata
     6841 ttactgcttc tggctgcatc aattatactt attttcttaa ggctggttct ggctgctggt
     6901 gtaacactga tagcagtatg tacacttgaa atattattgt tacgtcaatc tttaaggcaa
     6961 acgagggctc tggaaaacac gattgaagaa atttctgaat tgaacgaaaa aacaggggat
     7021 gtaataagca gattttcaca tcatatcagg gaaccactca acaacctggt cgttgttggt
     7081 gaaatgcttg ccagttctga actcagcaaa aaacaaaaag atcttgtcga aacattaata
     7141 gcatcaacaa acaacatggc cgacattctg aacgatctga cagtgcagag tgcagtggcc
     7201 ggctatgaag aaagaaaaca aatccggttc aacatagggg cggcaataga aaacactatt
     7261 gacctttttc gtttgaaaag tcctcagaat attgatttta ttttacataa aaaggatgca
     7321 gccaactttg aagtaacagg ggatcctata atattaaaac aaatatttct tgatattttt
     7381 aatattatca gtgaacagag cagtgagttt cttacaaaag taaccaccag tataaaaaag
     7441 gagaaagaaa aagaccgtga atgcattgta tcgttcagga tacaaactga tagaaggatt
     7501 atttttttag atgaccggaa tgccacaagc aatcacatga ttaaacttat cagttcagga
     7561 ggaggtaatt atgcccagga actgagtgag aattattcag tatttacttt cacactgaag
     7621 tttgtttcta ttgctgaaga gaagaaaaaa ccaatagctt caccagccat agaagcacta
     7681 agggttaagg aaaaacataa aaaagaactc aaagatgcca atattctcct tgttgaagat
     7741 aaccccataa accagaaaat tacattactc actcttaaac cgctggtaaa tagcattgat
     7801 actgcttctg acggaaggga ggctctcgaa aagttcggca gttcctccta tgatctgatt
     7861 cttatggata tacagatgcc agttatgagt ggaattattg ctgctgaaaa aataagggca
     7921 cttgaagcaa gcaccagcag gcatactcca attattgcta taacggcaaa tgctatgctg
     7981 ggtgacaaag aaaggtgcct tgcagcagga atggatgatt acataagcaa acccttccag
     8041 ccctcttcac taatcgatat aattaagaaa cacctctaaa acctagcaat tacagcctta
     8101 gtccgataac aagtatgtca tcaacctgtt cattatttcc tttccatgaa ttcagtgttt
     8161 cctccagaat tgtcttttgt tcgttaagag gtagcatgtt tattgttgta agaagatacc
     8221 tgaaacggcg gtacataaat ttcttgccct cctcacctcc aaactggtca gcatacccgt
     8281 cggtaaaaag atagacaact tcaccacctg aaaagttttc aacctgtgag gtaaatctga
     8341 acttctcagg cttcattccc agaatctgtt tgtctccttt aatctcctta agtctgttat
     8401 cctttatgaa atacatagat gaaaaagcac ccgcaaactc aataactttc cttttatgat
     8461 ctatcataca gatgccgata tccatactat ctctgatgta atactcccct tccctggaaa
     8521 aataatttct gactgataca tcaagccgtt ccataatttc agcaggatta tcagaattgc
     8581 cattgctgat aacctgattc aatatatttt gtcctataat tgacatcagt gcccccggta
     8641 ctccgtgacc ggtacagtcg gctgaaatca cacaaattct atcacccgta ataccaaacc
     8701 agtaaaaatc accgctaaca acatcgcgtg gctgataaaa tataaaatag tcattgaagt
     8761 gatttgcaag aaatgattcg gaaggaagaa gagctttctg aatatgacgt gcataaacaa
     8821 gactgtcagt aacattcctt aatttatatt ccagaattga caggttttcc tttgtttcat
     8881 tgagctctga aattgtactg gtgagagttt tccggtttct tatcaattcc ctccataaat
     8941 aaatcaatga tgcaattaaa ataaccgtaa tcagcagaaa aacaataaaa tataaattac
     9001 tgattgaatt taccttaccg ggattcaatg acgtctcaca gttcaattcc ctcagaaaat
     9061 taactgaaaa aacatcatat attattataa aagtaagaat cattctaatt caaaaataca
     9121 tttaatcgta gagaaggtaa agggtaaaat caataattta cggcattacg gttgccgaat
     9181 ttcccttccg taattttaag tatttcaatg atggagagtt cactaaatta cccattccgg
     9241 caacactcca tttttcatcg accgatgtta tagtaccttc atccgagcaa acgatatcag
     9301 gccagggcct tgggaaacct ccctgtctga aagctttcac cgtgccatca aaaagtattg
     9361 aagatgtccc aaaatacctg tggtccctca aaggatccga attaccaagc aactgccatg
     9421 ctaccatatt caaatcccct gtgtcaactt cttcatcaac ggcaagtaca acttttaaaa
     9481 catcccctgc tccggtatta ttcaggaatt gcacaatttt ttcaatattt acgagcttcc
     9541 cttccctttt tactgaaata acagctactg gatataatga cctatttctt aaaatatggt
     9601 atgatgaaat catctgatgt tttaccatct cattaagaac atctgtaatt tttttctcat
     9661 ctgttctgtt tttatttcca ggtagtgttt cagaaagcaa tttccttgtt gcatcgatac
     9721 ctgctttacc tccgaatgca gggacgtcgc atgaatggtc gagtacatcg agcggtccgc
     9781 gtgaaaaaat tacatccttt tttacatcag tattattaat tacctcctcg agaacctcac
     9841 tataatttct gatatcaatg ccatcgctta ctgcaatgat gtattttgag aacatcatct
     9901 gtcctgctcc taaaagcgaa ttaattacct taagtccctg tccgggataa ttttttttca
     9961 tactgactat caccaggtta tgtgccactc ctgcaggggg catatgcagg tcggttattt
    10021 cactgttaag caccatctgg agtggtttga gaaaaatttt ctcggtagct ctggtaaaga
    10081 aaagatcttc cttaggtggt atgcctacca ctgtagcggg atatacggca tcgcgggcat
    10141 gggtgataca ggtaacatga aactttggat acatgtcgga cagagagtag aagccggtat
    10201 ggtcgccgaa aggaccttct gaaaccaggg gttcggaagg gtcgacataa ccttcaatta
    10261 caatgtcggc atcttcagga acgtagatgt cgtttgtaac acattttacc atttttattc
    10321 tacgctgtgt aagaaatcct gcaaggatat attcgtcgat attttccgga aggggtgctg
    10381 ttgctgcata ggtataaaca gggtcgcctc cgagaacgac tgagacaggc atcattttcc
    10441 ctgtttcttt ccacagcctg taatggtttg ctcctgtttt atgaagctgc cagtgcatgg
    10501 ctgttgtgca tttgtcaagt atttgcatcc ggtacatacc ggcattgggt gcaagggtga
    10561 atgggttgta ggtatgaaca atcggaaggg tgataaatct tccaccgtcg tgcggccagc
    10621 atttcagaac aggaagtatt tcaaggtcag ggtcttttag aataacctgc tggcatcggc
    10681 ccttccggca tgttcttaca ggtatgaatt tcctcaggcg aaggagggta aaaaaatctg
    10741 aaattttatt taatgggtta cttcttcccg gtgcaagaag atcagatatt ttatcaattt
    10801 cgctgtatat attttccggc tcttccgttt taagtgccag attcatcctt ttttctgatc
    10861 caaaagcatt tatgagtacc ggaaatgctg ttccattgtt ttcgaataat agggccggac
    10921 cacctgaatt tattgcccgg tcggcaaact ctgcaatttc aagaacaggg tcaacgaaaa
    10981 cggatatcct tttaagttct ccctcccgtt caagcacatg gataaaacct gcaagtcctt
    11041 ttgatgtcat caggaaataa aaatgttttt ttgtatgggc taaattagta acattttcgg
    11101 gggaaaatac attttggtct gtaattggtt ttccaggatt tgtgtcaggg gtcaggaatg
    11161 gttgtttttt ctggtatttc ctgtccggac tggaatgaag gtgggtataa aaacagtaat
    11221 gggaactatt ctttcgatta gttcccactc accgtttgca gccgaagcca catacggtgg
    11281 caggctacag ttattctggc cggctgcctt tcaggtctta cgatctgagg gaggcaaccc
    11341 tgagctttcc ggcactgctt tttagggctg tgccttccag ccgtcctttc tcccgattac
    11401 tcgggattcc gggacgaagc gtcattcagg catgcgctga tcaaccccgt agggttgttc
    11461 agtttgcagg tttcatcgga ccgaagtcct ttgtctcctg cagccctgtg acccagtcgt
    11521 tgaaagagcg ttggcgtttt cagcgacctg ctgaacatcg ggaactcttt tgcagggttc
    11581 cttagttcag gcttcggctc tctgatgcgc agacccggag gtctgaccga acccttcaga
    11641 gtcacactta agcaaccctt tatcgggttc cacatctttg agcagccttt tatctctcgc
    11701 ctgctgaatc aaaaatagaa taagtatctc gaataataaa ataaaaaaca ccgttgttgt
    11761 aaacgcaata tcaacatggg ttattaacaa ttgttaataa ctctcgatgt tgataatgtg
    11821 ctaatattca gtgagatagc agattattct gctttatttg catcttcctc cgtagctgat
    11881 ccgctacttt tttcctggtc aggttctgct gtatcagtag ttacatcttt ggtggcatta
    11941 tcctgcggtg ctgttccagt tgctgcggca gctgtttcag ccgttcctgc agctttcttc
    12001 tttggagctc ttctgcgccg ggtagttgtc ttttccggtt ttgtttgagc cgccagtctg
    12061 gcttcattga aatcaaccag ttcaataatg cacatctcgg cattatctcc caacctgttg
    12121 ccggttttaa gtattcttgt gtaacctccg gggcgatctg caaccttggg ggagatttcc
    12181 ctgaagagtt ctgatacagc ctttttgtcc tgaagatacc tgaataccat tcttctggaa
    12241 tgtgttgtat cttctttgga tctagttata agaggttcaa tatatgaacg aagtgctttt
    12301 gccttggccg ttgttgtagt tattctctta ttcagaatta gcgatacagc catgttcgca
    12361 agcatagcct tcctg
//
LOCUS       DAOSNB010000036        12114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5230_length_12114_cov_4.867402, whole genome shotgun sequence.
ACCESSION   DAOSNB010000036 DAOSNB010000000
VERSION     DAOSNB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5230_length_12114_cov_4.867402"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLG42_02860"
     CDS             complement(<1..464)
                     /locus_tag="PLG42_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU30184.1"
                     /translation="MNINIRPATEKDFPDVLSLIKELAEFEKAPEKVTNTVDQMKQEQ
                     HLFRCYVAETETGEIAGMALFFFAYFTWVGKSLYLDDIYVRPKFRKQKIGTALLKKVF
                     EVACEENCKRVRWQVLKWNQPAIQMYRKYGADIDNEWLNCTFEHEEIKSLRDR"
     gene            complement(560..778)
                     /locus_tag="PLG42_02865"
     CDS             complement(560..778)
                     /locus_tag="PLG42_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOU30185.1"
                     /translation="MFVVVYDGTLFHAQMIKNLLENEGVESFLKDEIIGSRSGEIWRP
                     GGGVKVVVYVNDLMKALKVVEDYENSLK"
     gene            complement(814..1845)
                     /locus_tag="PLG42_02870"
     CDS             complement(814..1845)
                     /locus_tag="PLG42_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HOU30186.1"
                     /translation="MRNLIILIAITTLVIQSCSDKAKESLAAVNSYFETDDTNSVQTG
                     GIKLIKINTPAGEFNVWTKRIGNNPSLKVLLLHGGPAATHEYFECFDSFFPREGIEYY
                     YYDQLGSYYSDQPSDTSLWNIDRFVEEVEQVRIALKLDSSNFFLLGHSWGGILAMEYA
                     LKYQKNLKGLIISNMMASCPEYGKYADEVLAKQMDPAVLAEIKALEARKDFSNPRYME
                     LLIPNHYKKHICRLEEWPDPVNRGFKHLNYEIYLMMQGPSELGIAGKLATWDRKSDLK
                     NIKVPTLVIGSKYDTMDPAHMEWMASQFPHGSYLYCPEGSHMAMYDDQKTYFKGLVKF
                     IKSVDKGNF"
     gene            complement(1970..2917)
                     /locus_tag="PLG42_02875"
     CDS             complement(1970..2917)
                     /locus_tag="PLG42_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HOU30187.1"
                     /translation="MKDLKIASAQFEHRSGDKKYNLSVIEKLSGKASGEGAEIIAFHE
                     CSVTGYTFARHLTKEQMLALAELIPEGESISKLVDIAEKHNIIILAGIFEKDKNDNLY
                     NSYVCVDKNGLIAKARKLHPFINPNLQPGDYYCIFEIKGWKCSILICYDNNIIENVRA
                     VTLLGAQIIFMPHVTMCTPSPRPGAGLVNPELWKNREKDPTSLRIEFDGLKGRQWLMK
                     WLPARAYDNGVYVVFSNPVGMDDDQVRNGCSMIIDPFGDILAECRSFDDSYVTALITP
                     EKLALAGGYRYIMARKPELYRDIIGQIHKPEQKVIWLND"
     gene            complement(3025..4233)
                     /locus_tag="PLG42_02880"
     CDS             complement(3025..4233)
                     /locus_tag="PLG42_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOU30188.1"
                     /translation="MSKKIFKNPSFRRKLFIIIIILIILLGTVTGIVYFNLDKVPGEG
                     KIETEISYIFSDNIIQIPVTINGIPTHGIFDTGSSFCLLDEKSLDKFNVKVIPFFRSK
                     INKTGSYRWGIIKNLEINNFKINNLLVNLMDFKTDSSIFKCIPSEIILGMNLIKKANW
                     KFNFTKHSLKIYPKNYRITDFKAENFISFFYRMNLPFTYITINEKSHKVMVDFGCSGA
                     LMLAEDDYSGNEAIPYSNYQSYNIFGSYKNEGKALIDDFIFGNDTIPGVEINFTSPRF
                     SLVGIETFLKYSEVLINNAEKKIYLSGMKKSAGTKTVFFGHGFYVYQDGGKIKISSIY
                     KNSPAYHAGLNIGDTVLSINGIESSVLIPMDLCDFTELCKRFFYQDTVRVTVIKNGEI
                     LTKEIVKSEF"
     gene            complement(4313..5176)
                     /locus_tag="PLG42_02885"
     CDS             complement(4313..5176)
                     /locus_tag="PLG42_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOU30189.1"
                     /translation="MDNRIKSLILKIQCNEITEHIIYLKLADLSGNQKNAEILRRIGN
                     QELAHYRYWKTKTNTEIKPDKFRVFRTVMMAKLLGLSFVLKLMEKREGTGSSTYQKIA
                     EIYPESKRFAEEEAIHEKELLDMLDEEVLQYVGSIVLGLNDALVELTGALAGFTLALG
                     DNKLISLAGLVTGISAALSMAASDYLSSKAEGNAQAKKSALYTGMAYFITVILLILPF
                     LLISSKFISLAITLSTAVLIIFFFNFYIATARNLNFRERFTEMTIISLGVAALSFLVG
                     FFLKNLIGVDI"
     gene            complement(5271..6044)
                     /locus_tag="PLG42_02890"
     CDS             complement(5271..6044)
                     /locus_tag="PLG42_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU30190.1"
                     /translation="MSSKLNFRLILFRIWYWYISKTDKNARVLFMNYGYHDKNQQIAI
                     DKDDELNRYSIQLYHHLASEVEIENKSIVEIGCGRGGGLSYISKKFSPSSAVGIDLCH
                     KAISFCKRFYSDRKILFFKGDAQKLFLNNNSCDIVINVESSHRYNDMKAFLREVYRIL
                     KPGGYFLFTDFRQSDKIEDLKKHLEISNFEILKERFINNEVIAALELDDPRKRNLVKL
                     LAPKFLQKIALNFAAVKGSDSYNKFISNRFVYFSYVLRK"
     gene            complement(6250..9063)
                     /locus_tag="PLG42_02895"
     CDS             complement(6250..9063)
                     /locus_tag="PLG42_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOU30191.1"
                     /translation="MKTRRLILNVFALILISVITRAQYDFSSFPPVDPEIITGKLPNG
                     LTYYIKHNSEPQKRASFYFIQNVGALLEEDHQNGLAHFLEHMAFNGTERFPGKAIISS
                     LEKHGVAFGYNINAYTGYEETIYNLSDVPVDSPGLIDTCLMILYDWSDFITLSEKEIE
                     AERGVIIEEWRTRRDANFRMMQKYLPVILKGSRYEKRDIIGDINVIRNFKPENIKEFY
                     DTWYRPDLQAVAVVGDFDAPEMEKKVKEIFSQLKPSINPLPRPEFEIPYHNGMLYVLA
                     TDKEAPRNVVSIFMKHPSVKPSEKNLSYMRRKLIISLINNMMSTRISELLQKGNPPFI
                     TGYVNFSELVRGVDALVISVAANPNREDVALEAIYTETERARRHGFAKGELERAKANL
                     LTNYENYYKQKDKISNDDFINDMQEHYLNNEPLVSPDFEYEFAKSVIPSVSVEEVSEF
                     FKGLVRTDNSVIVVQGQEDENIRHLTEEEAKAIISKVLSSDIEPYQDIETSESLIREE
                     LNGSGVIKRVYLKQFDAVEWTLGNGAKVVYRKADYEKDNVLLTAYSLGGSSLYETDYL
                     PSATMLPALIGMYGLGEFDNVTLQKMLAGKKATASVSLGQITETINGSATPKDFETMM
                     QLLYLRFTKPRFDPEAHNAIMSRYAAFLSSMAKDPSKIMQDSVSLYLTNYNPRTVILN
                     NQMLEKVELEKIEKIYRERFSNAADFIFFIVGNIDENTAREMTEKYIGSLPSSNAKEN
                     FIDRGVRPPRGHFYRDVKIPLTVPKATVFISHSADFPYNAYNNVCLKVINNILDLVFT
                     EKVREEAGGTYGVSVSLSSQLHPYQNAQGLIMFDCDPERVDSLKKIVYSEIERLTSEG
                     PSKENLDKAVKNMLKTREESRLHNNYWSNVLYLYYYSGLNVNDPKNFEKILNKLTIRD
                     IKKVASSFFSKADVADIVFRPEN"
     gene            complement(9185..9799)
                     /locus_tag="PLG42_02900"
     CDS             complement(9185..9799)
                     /locus_tag="PLG42_02900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU30192.1"
                     /translation="MHIAIAGNIGSGKTTLAGLLAKHFGWIPHYEDVEDNPYLNDFYE
                     DMQRWSFNLQIYFLNSRFRQIIEIRKSEKTVIQDRTIYEDAYIFAPNLHAMGLMSTRD
                     YENYISLFKLMCTFIQPPDLLLYLRASVPTLVNQIQKRGREYESSISINYLKRLNERY
                     EAWIETYDLGKLLIIEADHYDFPNNPDHLSEVIDKINAELHGLF"
     gene            complement(9976..11499)
                     /locus_tag="PLG42_02905"
     CDS             complement(9976..11499)
                     /locus_tag="PLG42_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOU30193.1"
                     /translation="MTFITSIANSINKSRLRQIDFFRKNPEEIQEAVFRNLISRAAKT
                     EWGIMHEYSGIRTIKQFQERVPIQGYEELLPFIERLRNGEKNLLWPGAVKWFAKSSGT
                     TGARSKFIPITRDSLHECHYQGGKDVIALYLNNRPDSGIFYGKGLILGGSHKIDNFSN
                     RSLYGDLSAILIENTPWWFDIIRTPSRKIALIEDFEKKLELITRITVKQNVTSLTGVP
                     SWFLVLIKQILNYTGKTDLKEVWPNLEVFFHGGVSFKPYRELFRHFISDSQFRYVETY
                     NASEGFFGVQDDFSSNDMLLMLDIGIFYEFIPVNEISSGKNTALTIGEVSKGTNYAMV
                     ISTNGGLWRYLIGDTIQFTSLYPHKFIISGRTRHYINVFGEEVIVENADKAIESACNA
                     TGAIVLEYTAGPVFTGLSSKGSHEWIIEFEKEPGDLALFTEKLDEALKQVNSDYEAKR
                     FKDLNLTMPVVRSVPRGTFYNWLKEKNKLGGQNKVPRLSNSRDYIEELYRIAGITGA"
     gene            complement(11529..>12114)
                     /locus_tag="PLG42_02910"
     CDS             complement(11529..>12114)
                     /locus_tag="PLG42_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HOU30194.1"
                     /translation="MVIYYKETREIQILKEFSPLFIPAGIYGNMLKSSIALFTGEVLA
                     SVLREETPQKELFAYLVDSIKYLDLCESGIQNFHIGFLTGLCSFLGIEPGKRKDPEHV
                     FFDFSNGSFVPVPPPHGNYAPREVSDILAVVFNSSWNEIGNIPMTGILRNEVLSALLK
                     YFSLHFPSLKKINSLKIFREVFQTENEFKTFDNS"
BASE COUNT         3542 a         2267 c         2189 g         4116 t
ORIGIN      
        1 cgatccctga gacttttgat ttcttcatgt tcaaaagtac agttaagcca ttcattgtca
       61 atatcagctc cgtatttgcg gtacatctga atggcaggct gattccattt aagcacctgc
      121 caacgaaccc ttttgcaatt ttcttcacag gcaacttcaa atactttttt taagagagcc
      181 gttcctattt tttgttttct gaattttggt ctgacgtaga tgtcgtcgag gtaaagcgat
      241 ttgcctaccc atgtgaaata ggcaaagaaa aacagagcca ttcctgcaat ctctccggtt
      301 tcagtttcag ccacatagca tctgaacaaa tgttgttcct gtttcatctg gtcaactgta
      361 tttgttacct tttcgggtgc cttttcaaat tcagccagtt ctttaattaa tgaaagaaca
      421 tctggaaaat ccttttcagt tgcaggtctg atattaatat tcatatcctt atgattttga
      481 gtgtttcaaa acagaaaaat attgttgaat aaaaattaac cagattattg aaaaatacaa
      541 catttcagga agaagagtat tattttaaag agttttcata atcttccaca acctttaaag
      601 ccttcattaa atcattaaca taaactacga ctttaactcc gcctcctggt ctccatattt
      661 ctccgctacg gcttccgata atctcatctt taagaaaaga ttcaacacct tcattttcca
      721 gtagattttt aatcatttga gcatgaaaca gtgtaccgtc atatactact acaaacattt
      781 tcatttctgt tgtttgtctt ttattcttta atattagaaa ttgcccttgt ccacgctttt
      841 aataaattta accagccctt tgaaatatgt tttctgatca tcatacattg ccatatggct
      901 tccttcaggg caatacagat agctcccatg tggaaattgt gaagccatcc attccatgtg
      961 agcggggtcc atagtatcat attttgagcc aattaccaga gttgggactt taatattctt
     1021 cagatctgac tttctgtccc aggttgccag ttttcctgct attccaagct cactgggtcc
     1081 ctgcatcata agataaattt catagttcag gtgtttaaaa cctctgttaa ctgggtcggg
     1141 ccattcttca agcctgcaaa tatgcttttt ataatggttg ggtatcagca gctccatgta
     1201 tctggggtta ctgaaatctt tcctggcttc aagagctttt atttcagcca gcactgcagg
     1261 atccatctgt tttgccagga cctcatcagc atattttcca tattccgggc aacttgccat
     1321 catatttgaa attataaggc ctttgagatt tttctggtac ttcagagcat attccattgc
     1381 caggataccg ccccacgagt ggccaagaag aaagaagttt gaactgtcaa gttttaatgc
     1441 gattctgacc tgctcaactt cttccacaaa cctgtcaata ttccagagag acgtatcgga
     1501 cggctgatca ctgtaataag aacccagctg gtcgtaataa taatattcaa ttccttcgcg
     1561 ggggaaaaag ctatcgaagc attcgaaata ttcatgcgtt gctgcaggtc ctccgtgaag
     1621 cagaagaacc tttaatgaag gattgttccc tattcttttt gtccaaacat tgaattcacc
     1681 tgccggggtg ttgattttaa tcaattttat tccaccggtc tgaacactgt ttgtatcgtc
     1741 tgtctcaaaa tagctgttta ctgccgctaa agattctttt gctttatcag aacaggattg
     1801 aatcacaaga gttgtaattg caatcagaat aataagattt ctcatttttg tttcctgttt
     1861 tagtatagaa tgaaaataaa ttttccggtt aatttaaaaa ataattgcaa gcagaaaaat
     1921 agatagaagg ggtttattac agtatataga ccactgacag ttttagcttt cagtcgttta
     1981 gccagataac tttctgttca ggtttatgga tctggccgat gatatccctg tacaattctg
     2041 gttttcttgc catgatatat ctgtaaccac cagcaagtgc aagtttttcc ggtgtaatta
     2101 aggctgtaac gtagctatca tcaaatgaac ggcattcagc aagtatgtca ccgaaaggat
     2161 caattatcat tgagcatccg tttctgacct gatcgtcgtc catgccaaca ggatttgaaa
     2221 agaccacata tacgccatta tcataggccc ttgccggaag ccatttcata agccactggc
     2281 ggcctttcag cccatcaaac tcaattctta atgaagtagg gtccttttca cggtttttcc
     2341 atagttcggg attgaccagt cctgctcccg gtcggggtga aggcgtgcac attgttacat
     2401 gaggcataaa aattatctgg gctcccagaa gagtaactgc ccttacattt tcgattatat
     2461 tattgtcgta gcaaatcaga atactgcact tccatccttt gatctcaaaa atgcaataat
     2521 aatcacccgg ttgcagattc gggttaataa acggatggag ttttcttgcc tttgctatta
     2581 gtccgttttt atcaacgcat acatatgagt tataaagatt atcgtttttg tctttttcaa
     2641 aaattcctgc aagtattatt atgttatgct tttcagcaat atctacaagt tttgagatac
     2701 tttcaccttc aggaataagt tcagccaacg cgagcatctg ctcttttgta agatggcgtg
     2761 caaaagtata tccagtaaca gaacactcat gaaaagcaat aatttcagct ccttcacctg
     2821 atgcttttcc cgaaagtttt tctattactg aaagattgta ttttttgtct ccactccggt
     2881 gttcgaactg tgccgatgcg attttaagat ctttcataaa aaattaattt agaaacggga
     2941 ttctaccgga agtaaagata acctgatttt ttattcacaa tccccccaga gagaaagtat
     3001 atgaaggagt atattgctgc cgtttcaaaa ttcggattta acaatttctt ttgtcagaat
     3061 ctcaccgttt ttgattactg taactctaac agtatcttga taaaaaaacc tcttgcataa
     3121 ttctgtgaaa tcacataaat ccataggaat taaaactgat gattctatac catttatact
     3181 taaaactgta tcccctatgt ttaagcctgc atgataagcc ggtgaatttt tgtaaataga
     3241 gcttattttt attttcccgc catcctgata gacataaaat ccgtggccga aaaatacagt
     3301 ttttgttccg gcgctctttt tcatacccga caaatatatt ttcttttcag cattatttat
     3361 aagtacttca gagtatttga gaaaagtttc aatgccgact aatgaaaatc gtgggcttgt
     3421 aaaatttatt tccaccccgg gtattgtatc attgccaaaa ataaaatcat caattaaagc
     3481 tttgccttca tttttgtaac ttccaaaaat attatatgat tgataatttg aatatgggat
     3541 tgcttcgttg ccggaatagt catcttcagc caacatgagt gcaccggaac agccgaaatc
     3601 aaccataact ttatgtgact tttcatttat ggttatataa gtaaaaggaa gattcatcct
     3661 gtagaaaaaa ctgataaagt tttctgcctt gaaatcagtt atcctgtagt ttttaggata
     3721 gatttttaat gaatgcttgg taaaattaaa tttccagttt gcctttttta tcagattcat
     3781 tcccagtatg atttctgaag gtatgcattt aaagattgaa ctgtcggtct taaaatccat
     3841 cagattaaca aggaggttat taatcttaaa attgtttatt tctaaatttt tgattattcc
     3901 ccacctgtag gaaccggttt tgtttatttt gcttctaaaa aaagggataa ccttaacatt
     3961 aaacttatcc aggctttttt cgtcgagcag gcaaaagctt gaacctgtgt cgaaaattcc
     4021 gtgcgtggga atcccgttaa tggtaaccgg aatctggatt atgttatctg aaaagatata
     4081 ggaaatttct gtctcaattt ttccttcacc aggtacttta tcaagattaa agtacacaat
     4141 tcctgttact gttccaagaa gtattatcaa tatgataatg ataatgaaca gcttacgtct
     4201 aaacgacgga tttttaaata ttttttttga catgcagcaa gacttttttc acatatcgct
     4261 aaaatagatt agtttttcaa aaaatcaaat ttttctcaaa gtatttttta aatcaaatgt
     4321 caacaccgat taaattttta agaaagaatc ctacaaggaa tgataatgcc gctactccga
     4381 ggctgatgat agtcatttct gtgaatcgtt ctctaaaatt aaggttacga gcagttgcaa
     4441 tatagaagtt aaagaagaag ataatcagta cagctgttga aagagttatt gccagggaaa
     4501 taaattttga tgaaatcaag agaaaaggaa gaataagcag tattactgtt ataaaataag
     4561 ccattccagt gtacagggct gatttttttg cctgtgcatt gccctcggct tttgatgaaa
     4621 gatagtctga tgcagccatt gaaagtgctg ctgatatacc agttaccaat ccagccagtg
     4681 aaattaattt attgtcaccc agagccagtg tgaaacctgc cagtgctccg gtaagctcta
     4741 ctagtgcgtc attcagccca agcacgattg aaccaacata ctgaaggact tcttcatcaa
     4801 gcatgtcgag gagttctttt tcatgtattg cttcttcttc tgcaaagcgc tttgattcag
     4861 gataaatctc agctatcttc tgataagtac ttgaacctgt gccttccctt ttttccataa
     4921 gtttaagaac gaaagaaagg ccgagtagtt ttgccatcat aacagtgcga aataccctga
     4981 atttatcggg ttttatttcg gtatttgttt ttgtcttcca atagcggtag tgagcaagtt
     5041 cctgattgcc tatccggcga agtatttctg catttttctg gttgcctgaa agatctgcaa
     5101 gttttagata aataatatgt tctgttattt cgttgcactg gattttaaga atcaaggatt
     5161 ttatcctgtt atccatagtt tattaagaat ttgtaaacaa ctaagtaaat aattccttga
     5221 aaaaataaaa aatggaaaag tataattttt ataacgttat aattcagtta tcattttctt
     5281 aaaacataac tgaagtaaac aaatctgttt gaaatgaatt tattataact atcagaacct
     5341 tttacagcgg caaagttcag tgctattttc tgcagaaatt taggggcaag caattttact
     5401 agattgcgtt tgcgcggatc atccagttcg agagctgcaa taacttcatt atttatgaat
     5461 ctttctttta gaatttcaaa attgcttatt tccagatgct tttttaagtc ctctatttta
     5521 tcggactgtc ggaaatcggt aaataaaaag taacctcctg gctttaagat ccgataaact
     5581 tcccggagaa atgctttcat gtcattatag cggtgcgatg attccacatt aattacaatg
     5641 tcacaggaat tgttattaag aaataatttc tgtgcatcac ctttgaagaa caatattttt
     5701 ctatctgaat agaagcgttt acaaaatgaa attgctttat ggcataaatc tattccaaca
     5761 gctgaagaag gtgaaaactt ttttgatata tatgagagcc caccacccct gccgcagcca
     5821 atttcaacaa ttgacttgtt ttcaatttct acttcacttg ccagatgatg gtataactgt
     5881 atagaatagc gatttagctc atcatcttta tcaattgcaa tttgctgatt tttatcatga
     5941 tatccatagt tcatgaaaag taccctggca tttttatcgg ttttacttat gtaccagtac
     6001 cagattctga agagaatcaa tctgaaattt agtttggaag acataatata ggatatgtat
     6061 tattgggtgg attttgttat tttaagaatg agcggagaga tataatttac gtaaaaaagt
     6121 taatacaaat attgattaaa taggttccaa aggtaagtaa taatatttac gaattaatat
     6181 tctgtcaaga ttatttatat tttttgttta aagcgtgggg agggaaaaat gataatttta
     6241 aacttttcat caattctcag gtcggaaaac tatatctgca acatctgctt tactaaagaa
     6301 gctggaggcc acttttttta tatccctaat tgttagttta ttaagaatct tttcaaagtt
     6361 tttagggtcg ttaacgttaa gacctgaata gtaataaagg tagagaacat tgctccagta
     6421 attattatga agcctggact cttcgcgggt tttcagcata tttttgacgg ctttatctag
     6481 gttttccttt gatggtcctt cagaagtaag tctttctatt tcgctatata caattttttt
     6541 cagagaatca actctctcag ggtcgcagtc gaacataata agcccctggg cattttgata
     6601 cgggtgaagc tgagaactta gacttactga aacgccgtaa gttcctcctg cttcttctct
     6661 tactttttct gtaaaaacaa gatcaagaat attattaatg actttgaggc aaacattatt
     6721 gtaagcatta tatggaaaat ctgccgaatg gcttataaac accgttgctt ttggaacggt
     6781 aaggggtatt ttcacatctc tatagaaatg tccacgagga ggtctgacac ccctgtcgat
     6841 aaaattctct ttagcatttg aagaagggag tgagcctatg tatttctcag tcatttctct
     6901 agctgtattt tcatcgatat ttcctactat gaagaagata aaatcagctg cgttgctgaa
     6961 cctttccctg tatatttttt caatcttttc cagctcaact ttttcgagca tttgattgtt
     7021 aaggattacc gttcttggat tatagtttgt aagatagagt gaaacggaat cctgcattat
     7081 tttcgaaggg tcttttgcca tacttgaaag gaatgctgca tatctcgaca taatggcatt
     7141 atgtgcttca ggatcgaatc ttggttttgt gaatctcaga taaagcagtt gcatcattgt
     7201 ttcaaagtct ttgggtgtgg cggagccatt gattgtttcg gttatttgtc caagagaaac
     7261 tgaagcagta gcctttttgc ctgcaagcat tttctgcagg gtgacattat caaattctcc
     7321 gagtccgtac attccgatca gagcagggag catggttgcc gatggtaaat agtctgtttc
     7381 gtacaatgag gatcctccca gactatatgc ggtaagtaga acattatctt tttcataatc
     7441 tgctttgcgg taaacaactt ttgctccgtt gccaagtgtc cattctactg cgtcgaactg
     7501 tttgagatag acccttttaa tcacacctga gccattaagc tcttctctga taagtgattc
     7561 tgaggtctca atatcttgat atggttctat gtcagatgat agcaccttgc ttattatggc
     7621 ttttgcctct tcttcagtga ggtgccgtat attttcatct tcctggccct gaacaacaat
     7681 cacactgttg tctgtcctta ccaggccttt aaaaaattca gatacttctt caactgaaac
     7741 agatgggata acactttttg caaattcata ctcaaaatcc ggagaaacaa gaggctcatt
     7801 gttgagataa tgctcctgca tatcgttaat gaagtcatca tttgaaatct tgtctttttg
     7861 tttgtagtaa ttttcgtaat tggttaacag gttagctttg gccctttcaa gttctccttt
     7921 tgcaaatcca tgtctgcgtg cacgctccgt ctcagtgtaa atagcttcaa gagctacatc
     7981 ttccctgtta gggtttgcag ctacacttat caccagagcg tctacgcccc ttaccaattc
     8041 tgaaaaattt acatatccag ttataaacgg aggattacct ttctggagta attcgcttat
     8101 tcttgtcgac atcatattgt ttataagact gatgatcagc tttctcctca tatatgaaag
     8161 attcttctca gaaggcttta ctgagggatg cttcataaag attgatacaa catttcttgg
     8221 agcttcttta tcagtggcaa gaacatataa cattccgttg tgataaggga tctcaaattc
     8281 aggtcttggc agaggattta tggatggttt aagttgtgaa aatatttcct ttactttttt
     8341 ctccatttcc ggggcatcga aatctcccac aactgcaaca gcttgtagat caggtctata
     8401 ccatgtgtca tagaactctt tgatgttttc cggtttgaaa tttcttataa cgttgatatc
     8461 tccaataata tctctttttt catatctgga acctttaagt attacaggca gatatttctg
     8521 catcattctg aaattggcat cccttcttgt tcgccattct tcgattataa cgccgcgttc
     8581 ggcttcaatt tccttttccg aaagagtaat aaaatctgac cagtcgtata gaatcatcag
     8641 acaggtgtca ataaggcctg gagaatcaac aggaacatca ctgaggttat atattgtttc
     8701 ttcatatccg gtgtatgcgt ttatgttata tccgaaagcc actccatgtt tttcgagtga
     8761 gcttataatt gcttttccag ggaaacgttc agttccatta aaagccatat gttcaagaaa
     8821 atgagcaagg ccgttctgat gatcttcttc aagaagggcc cctacattct gaatgaaata
     8881 aaaacttgct ctcttctgtg gctcactgtt gtgcttaatg taatatgtaa gaccattcgg
     8941 aagttttcct gttattattt cagggtcaac aggaggaaag gaggaaaagt cgtactgtgc
     9001 ccgggtgatt accgatatta aaataagtgc aaatacgttc aggattaatc ttctggtttt
     9061 cataaaatta tgttaattgc ctggatataa cgaggatatt agtttttaaa taaagataag
     9121 gatatatatt caaatttcca ttattaaaaa atcaaatagg taaaatcaaa cgcatgagaa
     9181 tttttcagaa caagccgtgc aattcagcat ttattttgtc aatgacctca ctaaggtggt
     9241 cggggttgtt tgggaaatcg taatggtcag cttctattat cagaagtttt ccaaggtcat
     9301 atgtttcaat ccatgcctca tatctttcgt ttagcctttt aaggtagtta atgctgatag
     9361 agctttcata ttccctgcct cttttctgga tctggttaac aagggtgggt actgatgccc
     9421 tcaggtaaag aagaagatca ggtggctgga taaatgtgca catcagttta aaaaggctga
     9481 tataattctc gtagtcacgt gttgacatta ggcccattgc atgcaggttt ggtgcaaaaa
     9541 tataagcatc ttcgtagatt gtgcggtcct gaataacggt tttctcagat tttcgtatct
     9601 caataatttg tctgaagcgg gaattcaaaa aatagatctg cagattaaac gaccaacgct
     9661 gcatatcttc ataaaagtcg ttcagataag gattgtcttc cacatcctca taatgtggaa
     9721 tccatccgaa gtgctttgcc agaagtcctg caagggttgt tttgccggat ccaatatttc
     9781 ctgcaattgc gatgtgcata cattagttaa attagaaccg taaatttaga aataaaatga
     9841 cagatatttg atatttttta aagtttaaaa atttttcatg tgcattcaga ttaaaattac
     9901 tattaacatt ttagatcttc aggttggttt aaatataaaa gtcatgcgaa attggttatt
     9961 gtactcaatt cactatcagg caccagtaat tccagcaatt ctgtaaagtt cttcaatata
    10021 atcacggctg tttgagaggc gagggacttt attttgacct ccaagtttgt ttttttcttt
    10081 cagccagttg tagaaagttc ctcggggtac gcttctgaca actggcattg taaggttaag
    10141 gtctttgaaa cgtttggctt cataatcgga atttacctgt ttaagtgctt catcaagttt
    10201 ttcggtaaaa agggcaaggt cgcctggttc tttttcaaat tcaataatcc attcgtgcga
    10261 gccctttgaa gataaaccgg taaatacagg ccctgcagta tattcaagta ctattgctcc
    10321 tgttgcatta caggcacttt caatagcctt atcggcattt tcaacaatta cttcttcccc
    10381 gaaaacattg atatagtgcc gggttctacc tgaaataatg aatttgtgag ggtatagaga
    10441 tgtaaactga atggtatcac ctattaagta tctccataag cctccgtttg tggatattac
    10501 catggcataa ttggttcctt ttgacacttc tccaattgta agtgcagtat tttttccgga
    10561 actgatttcg ttaaccggaa taaattcata aaatatgcca atatcaagca tcagcagcat
    10621 atcgttcgat gaaaagtcat cctggacccc aaaaaaacct tcggatgcgt tgtaggtttc
    10681 gacatatctg aattgtgagt cgctgatgaa atgcctgaaa agttctctgt aaggtttgaa
    10741 gctgactcca ccatggaaaa agacctcaag atttggccaa acctccttca ggtcagtttt
    10801 tccggtgtaa tttaaaattt gctttatcag taccagaaac catgatggga caccggtaag
    10861 gctggtaaca ttttgtttga cggttatacg ggttatcagt tcaagttttt tttcaaaatc
    10921 ttcaattaaa gcgattttcc tggatggggt tctgataatg tcgaaccacc atggagtatt
    10981 ttcaataagt attgctgaaa gatctccata caatgacctg ttgctgaaat tgtctatttt
    11041 atgactgcca ccgaggatca agccttttcc gtaaaaaata cctgaatcag gacggttgtt
    11101 aagatataat gcaataacat ctttccctcc ctggtaatgg cattcatgga gtgaatccct
    11161 ggtaatagga atgaatttgc ttcttgctcc ggttgtcccc gaagattttg cgaaccattt
    11221 tacagcccct ggccatagaa gattcttttc accgtttcta agtctttcaa taaatggcaa
    11281 taattcttcg tatccctgaa tggggactct ttcctggaat tgtttaatgg ttctgatgcc
    11341 tgaatattcg tgcatgatac cccactctgt ctttgcggcc ctgcttatta aattcctgaa
    11401 aacagcttcc tgaatctctt caggattctt cctgaagaag tctatttgcc tcaacctgct
    11461 tttattgata gagtttgcta tagaagtaat aaatgtcatt tttcagagag aaaatgctaa
    11521 atagtttttc aagaattatc aaaggttttg aattcatttt cagtttgaaa cacttccctg
    11581 aaaattttca gtgaatttat ttttttcaga gatgggaaat gtaaagaaaa atatttaagc
    11641 agtgcactta acacttcatt cctcaaaatg cctgtcatag gaatatttcc gatttcgttc
    11701 catgatgagt taaaaacaac ggcgaggata tccgatactt cccgtggagc atagtttcca
    11761 tgaggtggtg gtaccggaac aaatgatcca tttgagaaat caaaaaaaac atgttctgga
    11821 tcttttcttt ttccgggttc gattcccagg aaactgcaca atccagtcag aaagccaata
    11881 tgaaaattct gaattccaga ttcacacagg tcgagatatt ttatcgaatc gaccagataa
    11941 gcaaaaagtt ctttttgcgg cgtctcttcc ctcagcactg aagcaagtac ctctcctgta
    12001 aataatgcta ttgaactctt gagcatatta ccatatatcc cggcaggaat gaataaagga
    12061 gaaaactctt taaggatctg gatttcacgg gtttctttat aataaattac catg
//
LOCUS       DAOSNB010000037        11999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5283_length_11999_cov_4.920462, whole genome shotgun sequence.
ACCESSION   DAOSNB010000037 DAOSNB010000000
VERSION     DAOSNB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_11999_cov_4.920462"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLG42_02915"
     CDS             <1..677
                     /locus_tag="PLG42_02915"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOU30195.1"
                     /translation="DICTRSCRFCATKTGKPLPPDENEPARLADAVKKLGLSHCVITS
                     VDRDDLPDGGASFWAKTITEVKKENPGITIEALIPDFDGKEENIKKVIDTQPDILSHN
                     LETVKRLTPLIRTKAKYDRSLSVIRFIAGSGIPAKSGLMLGLGETEEEILETMDDLLE
                     AGCSILTLGQYLKPADGYMEPVAYISPEKFAEYRKIGLSKGFTFVESGPLVRSSWHAE
                     KHIGAR"
     gene            717..1424
                     /gene="lipB"
                     /locus_tag="PLG42_02920"
     CDS             717..1424
                     /gene="lipB"
                     /locus_tag="PLG42_02920"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOU30196.1"
                     /translation="MSYSVKYEDIGLKDFKEVWDYQTKIFNRMVEEKKSAFQSSEKVS
                     LVSPGTLIFVEHPHVYTLGKNGSENNLLLNYIQLQAKNASFYRIDRGGDITYHGPGQI
                     VGYPIFDIEAIGTNLKGYIHGLEEAVIRTLAFFGIKGERLEGATGVWLDSSMREKSRK
                     ICAIGVRASRYISMHGFAFNVNTQLEYFNYINPCGFTDRGVTSLEKELGKKQDMEEIK
                     KYLRKSLAEVFDLQFIN"
     gene            1432..3159
                     /gene="recJ"
                     /locus_tag="PLG42_02925"
     CDS             1432..3159
                     /gene="recJ"
                     /locus_tag="PLG42_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOU30197.1"
                     /translation="MLEKRWVIKERGDCAVVKRLKEDLEKEDLKIPESIANLMVQRGI
                     LTPKDAHLFLYPSLDNLHDPFLMKGMNIAVDRISSAISKNEKILVYGDYDVDGTTAVA
                     MMYSFLRQFSPNVLYYVPDRYREGYGVSFEGIDYAVDNNCKLIITLDCGIKATDKVKY
                     ARGKGLDVIICDHHYPGSEIPCAVAVLDPKQEGCSYPYKELSGCGVGFKLIQAYSRVH
                     GIPFSEITHFLDLVAVSIASDIVPITGENRILTYYGLKQLNQSPRVGLREIIRESEIS
                     RELVVEDVVFRIGPRINAAGRMETGGKAVNLLISEDPRLAADISKEISSYNAERRNVD
                     RIITTEAMRMISDDPRSMNAKTTVLYNPNWRKGVIGIVASRLIEIYYRPTVILTESNG
                     FATGSARSVQGYDLYQAIEACSDLLESFGGHMFAAGLTLKKENIERFRERFEEYVSKT
                     ITEEQLMPNIFIDSEISFDEITEDFYNGINLFQPFGPENMSPVFMARNVCDTGSGRMV
                     GSTGEHLKLELRQAGNGNKILQAIAFGQSDHFEYIRSGKPFDICFSVEMNEFRGNRNL
                     QLNIKDMKC"
     gene            complement(3205..5106)
                     /locus_tag="PLG42_02930"
     CDS             complement(3205..5106)
                     /locus_tag="PLG42_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30198.1"
                     /translation="MNEEIISKVAEEISSAEENATVLESLTDKLYELESDPVRINSGN
                     FRESARLFFLSEYQIRALTEYVRTNGKIVSPNEISNIRGFDVTTARMILPFITFGDTH
                     GALARRLWFRQTMLTSISLKPGTHDTTAAGSQIKALTKYRIETGHFSAGFTAEKDAGE
                     KFLSGNPPLPDFLSGYISYTGNGLIKKVIAGDFSARFGIGTIINTGFRAGMSVAEPGF
                     ISSNCEIRPYTSTDENNFFRGAAVVLGGKDAEVTFFASRNRIDATTVTDCDSVNTYIR
                     SLYTAGLHNTPGTLLKKDIVTESAWGINISGYFRNFTAGMLFTESTFSLPFMPSKSEP
                     KDIFEFSGRKNLLAGFYYSAAIQRFILSGEAASGTDLHPSVVQSLTFVPGDRISFSFL
                     YRYYSPAFCSFHGKGIFSGSTPANEEALSGTVKFEAARRLFISAGSEIKRSMWVKYLA
                     NFPSQSIRSEVRVKYLSEKLNIEALYNYHLSQSNLNELTGMPDIKEMKTNSFSLRLKY
                     VPSEYFTSVTRADMKICTGNEAPGMLLSQDAILRFRVLPLRVWFRHCIYSTGGWDTRI
                     YIYENDLVNSFSIPALSGKGTRTYLMAEWNIAGRVGLRFRYGITSKIVNADVEEFTDE
                     IKFQVRVRL"
     gene            complement(5207..5998)
                     /locus_tag="PLG42_02935"
     CDS             complement(5207..5998)
                     /locus_tag="PLG42_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30199.1"
                     /translation="MLLLSAVATADGFYRPAAGAGEAGMAYVCIMKPGLWSSFHNQAS
                     LSYYHNAAFSFSYENRFAIKELAVKSACLIIPAGSGSAGIFYSRSGFTGYRRETIAMA
                     SGLKLASDLSAGVQIDLFAEHQQGEYKDYLQVSCEAGLIYEPSRDIRVGIHIFNPIPN
                     SLRNIPMPSSIRAGAGMTISEGLFAGFEAELVSGRKPVFRSGFEYATGKKAILRCGYS
                     TAEPSFTFGIGFLTGPASVDIAFSTHPRLGITSGLTASFVLGKNP"
     gene            6334..7197
                     /locus_tag="PLG42_02940"
     CDS             6334..7197
                     /locus_tag="PLG42_02940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU30200.1"
                     /translation="MEIKTDLYLGDCKEKLKLIPDNSVDLIFTSPPYADQRRNTYGGI
                     HPDKYVEWFLPISLQLYRVLKPTGTFVLNIKEKVVNGERSTYVIELILEMKKQGWLWT
                     EEFIWHKKNSYPGKWPNRFRDAWERLLQFNKSRKFNMYQDQVMIPMGEWARTRLRKLS
                     ETDKIRDNSKVGSGFGKKISNWLNREKVYPTNVLHLATECSNKNHSAAFPEELPEWFI
                     KLFTKEYDIVLDPFMGSGTTLIVANRMRRHSIGIDIVPEYFQMVKKQLKPVELILLEP
                     ESEIYNEKIKP"
     gene            7178..7915
                     /locus_tag="PLG42_02945"
     CDS             7178..7915
                     /locus_tag="PLG42_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HOU30201.1"
                     /translation="MRKLNLKDVLEYVEKNIETFHSKRIQSLDSLRLTQVLKRKNPYL
                     FKAKNVLTAEQIVKGIVDAHISSNEETIFGEWLEGLAIFINKKVFGGYKSGIKGIDLE
                     FDKNGIRYIVTIKSGPNWGNSSQIAKMVEDFRTAKRTLRTNNSNLNIVAVNGCCYGRD
                     NRPDKGDYFKYCGQKFWEFISNNKNLYTDIVEPLGYKAKEKNDEYNKVYSQMINKFTK
                     EFVLNFCKANGEIDWEKLVRFNSAENV"
     gene            complement(7986..8492)
                     /gene="purE"
                     /locus_tag="PLG42_02950"
     CDS             complement(7986..8492)
                     /gene="purE"
                     /locus_tag="PLG42_02950"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOU30202.1"
                     /translation="MEPKVSIIMGSTSDMPVMEGAAKILNDFRIPFEIYALSAHRTPE
                     EVEKFARNAASRGIKVIIAGAGMAAHLPGVIAAMTTLPVIGVPIKASLEGLDSILSIL
                     QMPSGVPVATVGINAAQNAGILAAQIIATGEPEVAEEIKKFKEGLKKKVVQANEELKN
                     VKYPYKTN"
     gene            8578..9114
                     /gene="hpt"
                     /locus_tag="PLG42_02955"
     CDS             8578..9114
                     /gene="hpt"
                     /locus_tag="PLG42_02955"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOU30203.1"
                     /translation="MKKINILGKEFIELIPAGAIEKRIEEMAGEINRDYRGKEVIFIG
                     ILNGAFLFAASLFKKITLDAHITFVKLASYRGTRSSGEVKELIGWNEDISGKDVIVLE
                     DIVDTGVTLERVIDEIKIRKAGSVKVATLLLKPEACKKDVQVDYVGFKIPNKYVVGYG
                     LDYEGYARNLPSIYKMVE"
     gene            9145..9723
                     /locus_tag="PLG42_02960"
     CDS             9145..9723
                     /locus_tag="PLG42_02960"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOU30204.1"
                     /translation="MVNFLIFGPPGSGKGTQSVRLAEKFNLLHLSTGDMLRAEIAAGT
                     ELGKRMSAIMAKGELVPDEVVIEMIASKIDGAKGYAGFLYDGFPRTVAQAVALEKMLN
                     QRGMKIDCMLVLEVDRDELVKRLVLRAEQSGRPDDKDPAVIDNRIEVYREKTEPIISY
                     FKEKGIYQPVNGVGEIEDIFHRLSDIVVKYLN"
     gene            9774..10763
                     /gene="obgE"
                     /locus_tag="PLG42_02965"
     CDS             9774..10763
                     /gene="obgE"
                     /locus_tag="PLG42_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOU30205.1"
                     /translation="MAESNFVDYVKIFCRSGRGGAGSAHFRREKFVPKGGPDGGDGGR
                     GGHIILRGNEQLWTLLHLKYHRHFFAGNGEPGGRQQSTGASGKDVVIEVPLGTVVRNA
                     DTGEFLFEITRHNEEKILLQGGRGGLGNVHFKSPTNQTPRYAQPGEPGAEATVILELK
                     ILADVGLVGFPNAGKSTLLSVVTAAKPKIADYPFTTLEPNLGIVSYRDGRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSVLLFMVPVDSVNIKAEYEILLNELRCYNPELLDK
                     KKVLAITKSDLIDEEIKKEISKNLPDIPWVFISSVTGQGIDTLKDLLWKVLNN"
     gene            10768..11358
                     /locus_tag="PLG42_02970"
     CDS             10768..11358
                     /locus_tag="PLG42_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOU30206.1"
                     /translation="MLEALDRQLFLFLNSLNSPFWDHVMFIISAKLTWVPLYLAIIYA
                     LWRKYKRKLIVILLFVILAVTLSDRLSVAVKNTVKRPRPCHEKSLEGMVHTVKGKCGG
                     QYGFVSSHASNSFMVALFSLLLIRKRWYTVSIILWALVVGYSRIYLGVHYPGDVVAGS
                     ALGALAGWFTIFLYNLTDSRFLQKSGYFSQNKINKA"
     gene            11358..11990
                     /locus_tag="PLG42_02975"
     CDS             11358..11990
                     /locus_tag="PLG42_02975"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30207.1"
                     /translation="MSGTVLETYDPFFNLAVEDYLLHNSNKEYCLIYINDPSVVVGRH
                     QVIYRETNYFTAEKLNIPVIRRISGGGTVYHDRGNLNYSFIINSAKGMQVDFVRHTRP
                     VIEFLNINGIPAVLSGKSDITVNGFKVSGNAEHVFRERVLHHGTLLFDASLETMNSLL
                     RGDAVGYSTKAIGSNRMQVMNLKGRIINIETTEELAIKFFEHSLQGSSVT"
BASE COUNT         3700 a         2292 c         2629 g         3378 t
ORIGIN      
        1 gtgatatatg cacaaggtcg tgccgttttt gtgccacaaa aaccggtaag cccctgccgc
       61 ccgacgaaaa tgaacccgcc cgccttgccg acgctgtaaa aaaacttgga ctttcacatt
      121 gtgtcattac ttcagttgac cgcgacgacc tgcccgacgg cggagcctct ttctgggcaa
      181 aaactatcac cgaggttaaa aaagaaaatc ctggtattac cattgaggca cttattccag
      241 attttgacgg taaagaggaa aatataaaaa aagtcattga cacccaacct gatattcttt
      301 cccataacct tgaaactgta aaaagactaa cgccacttat acgtacaaag gcaaaatacg
      361 acagaagtct ttcagttatc agatttattg ccggttcggg tattccggca aaatcaggtt
      421 tgatgctcgg tctgggtgag acagaagaag agattctgga aacgatggac gaccttcttg
      481 aggctggctg ttccattctg accctgggac aatatcttaa gcctgccgat ggatacatgg
      541 aacccgtagc atatatttca ccggaaaagt ttgcagaata ccgtaaaatc gggctttcaa
      601 aaggattcac attcgtcgaa agcggacccc ttgtacgttc ttcatggcat gctgaaaaac
      661 atatcggagc aagataaaaa tacatatatt tacatatcat tgaaaaaagt ttcccattga
      721 gctatagtgt taaatatgag gatattgggc tgaaggattt taaagaagtc tgggattacc
      781 agacaaaaat tttcaaccgt atggttgagg aaaaaaagag tgcctttcag tcatctgaaa
      841 aagtttctct ggtttcaccg ggcacactca ttttcgttga acatcctcat gtttatactc
      901 ttgggaagaa cggttccgag aacaatctgc tgctcaatta catacagctg caggcaaaga
      961 atgcttcttt ctaccgcatc gacaggggag gagatataac ctatcacgga cccggccaga
     1021 tagtcggata tccgattttc gacattgaag ccatcggcac caatctgaag ggttatatac
     1081 acggacttga ggaagcagtg atacgaaccc ttgctttttt tggaataaag ggcgaacgcc
     1141 tcgaaggtgc aacaggcgtg tggctggaca gcagcatgag ggaaaagtca cggaaaatat
     1201 gtgcaatagg tgtacgcgcc agccgttata tatcaatgca cggttttgct tttaacgtaa
     1261 acactcaact tgaatatttt aattatatca atccatgcgg atttaccgac cgtggagtga
     1321 cttcgctcga aaaggaactg ggcaaaaaac aggatatgga agaaattaaa aaatatctca
     1381 ggaagagcct tgcagaagtt tttgaccttc aatttattaa ctgaagaaga tatgctggaa
     1441 aaaagatggg tcatcaagga aagaggcgat tgtgctgttg ttaagaggct gaaagaagat
     1501 cttgagaaag aagatcttaa gataccggaa tctattgcca atctgatggt acagcggggt
     1561 atccttacac ccaaagatgc tcatctgttt ttatatcctt cactcgacaa tcttcatgat
     1621 cctttcctta tgaaaggaat gaatattgca gtcgaccgta tctcctcagc tataagcaag
     1681 aatgaaaaaa tacttgtcta tggcgactat gatgttgacg gtacaacggc tgtagcaatg
     1741 atgtattcat tcctcaggca gttctcaccc aacgttcttt attatgtccc cgaccggtac
     1801 cgggaaggtt atggtgtgtc gtttgaaggc attgattatg cagtagataa taattgcaaa
     1861 cttattataa ccctcgattg tggtataaag gcaacagata aggtaaagta tgccagagga
     1921 aagggcctcg atgtaatcat ttgcgaccat cattaccctg gcagcgaaat accatgcgcc
     1981 gttgcggtac ttgatcctaa acaggaagga tgcagctatc cctataagga actatcgggt
     2041 tgcggagtgg gctttaaact catacaggca tactctcgtg tgcatggaat cccattcagt
     2101 gaaattactc atttcctcga ccttgttgca gtgagcatcg catcggatat agtgccaata
     2161 acgggtgaaa acagaatcct tacatattac gggcttaaac aacttaatca gtcaccaagg
     2221 gtgggtctaa gggaaataat aagagaatca gaaatctcca gggaactggt tgtggaagat
     2281 gtggtattca gaatcggtcc gaggattaat gccgcaggca ggatggaaac gggcggcaaa
     2341 gccgttaacc tgctgatctc tgaagacccc agactggcag cagacatcag taaggagata
     2401 agcagctaca acgccgagcg ccgcaatgta gaccggataa taacaaccga ggcaatgaga
     2461 atgatatccg acgaccctcg aagtatgaat gccaagacta cagtattgta taatccaaac
     2521 tggcgtaaag gcgttatcgg aatagtagct tccagactta ttgaaatata ttaccggcca
     2581 actgtgattc taaccgaatc gaacggattc gctacaggat ctgcacgttc cgtacaggga
     2641 tacgaccttt atcaggcaat agaagcatgc agcgaccttc tagagagctt cggcggacat
     2701 atgtttgccg ccggactgac actgaaaaag gaaaatattg aacgcttcag ggaaagattc
     2761 gaggaatatg tcagtaaaac cataaccgaa gaacaactga tgccaaatat ttttattgat
     2821 tcagaaataa gctttgatga aataacggaa gatttttaca acggaatcaa tcttttccag
     2881 ccattcggcc ctgaaaatat gtcgcctgta tttatggcaa gaaatgtatg tgacacaggt
     2941 tctggaagga tggttggttc aacgggagaa cacctcaaac ttgaactccg tcaggcaggc
     3001 aacggcaata agattcttca ggctatagct ttcggacagt cggaccattt cgaatatatc
     3061 cggtcgggaa agccgttcga tatctgtttc tcggtagaga tgaacgaatt ccggggcaac
     3121 agaaaccttc agctcaatat aaaagacatg aagtgctgaa accggctcac aaatactgaa
     3181 attactttcc aatctcccag cttttcataa ccttaccctg acctgaaatt taatttcatc
     3241 ggtaaattct tcaacgtcag catttactat ttttgaggta ataccatatc tgaaccgcaa
     3301 tccgactctt ccggcaatgt tccactcagc catcagatat gttctcgttc ctttgcccga
     3361 taaagcagga atactgaatg agtttacgag gtcgttttcg tagatataga ttcttgtatc
     3421 ccatccgcct gtgctgtaaa tgcaatgcct gaaccacact cgtagcggta aaactctaaa
     3481 cctcaatata gcatcctgac ttagcaacat accaggcgct tcattgccag tgcatatctt
     3541 catatcggcc cttgttaccg aggtaaaata ctctgaagga acatatttca acctcaggga
     3601 aaaggaattt gttttcattt cctttatatc aggcatacct gtcaattcat tgaggttcga
     3661 ttgggaaaga tggtaattgt acagggcttc aatatttaat ttttcggaaa ggtatttcac
     3721 tcttacctcg ctgcgtatgg attgtgatgg aaagtttgca agatatttaa cccacataga
     3781 cctttttatt tcactgccag cacttataaa tagccttctg gcagcttcaa attttacagt
     3841 gcctgataac gcttcttcat ttgcaggtgt tgaacctgaa aagataccct tgccgtgaaa
     3901 gctgcagaaa gctggagaat agtatctgta aaggaagctg aaagaaatcc tgtcgccagg
     3961 tacgaaagta agtgattgga caacagacgg atgaaggtcg gtccctgaag ctgcttcccc
     4021 tgaaagaatg aacctttgta ttgcggcact gtaatagaat ccggcaagga ggttctttct
     4081 tcctgaaaac tcaaagatgt ctttgggttc tgattttgag ggcatgaaag gcaatgaaaa
     4141 agtactttcg gtaaacagca tgcctgcagt aaaattcctg aaataacccg aaatgtttat
     4201 accccatgca gattctgtga cgatatcttt tttcagcagg gtaccgggag tattatgcaa
     4261 tcctgccgtg tagaggctcc ttatataagt attaacagag tcacagtctg ttactgttgt
     4321 tgcatctatt ctgttgcgtg atgcaaagaa ggtcacttct gcgtcttttc ctcccagcac
     4381 cactgctgct ccacgaaaga aattgttttc gtccgtagag gtataaggcc gtatctcgca
     4441 gtttgacgaa ataaaacccg gttcggctac cgacatcccg gccctgaacc ccgtatttat
     4501 aatggtacca atgccgaacc gggctgaaaa atcgcctgca atcaccttct ttatcagtcc
     4561 gtttccggtg taactgatat aacccgatag aaaatcaggc aaaggaggat taccgctgag
     4621 gaatttctcc cctgcatctt tttcggctgt aaaccctgcc gaaaaatgtc cggtttcaat
     4681 cctgtatttt gtgagtgcct ttatctgtga acctgctgca gttgtgtcgt gcgtgccagg
     4741 tttcaatgaa atactggtaa gcatagtctg tctgaaccac aggcggcgcg cgagagctcc
     4801 atgtgtgtct ccgaaagtta taaacgggag tatcatcctg gctgttgtaa catcgaatcc
     4861 tcttatattt gagatttcgt tcggggagac tatcttcccg tttgttctga catattccgt
     4921 aagagccctt atctgatatt ctgaaagaaa gaagagtctg gcactctccc tgaagtttcc
     4981 cgagttgatt ctcaccggat cactctcgag ttcgtaaagt ttgtcggtga gtgattcgag
     5041 aacagttgca ttctcttcag ctgaagaaat ctcttctgca actttcgaaa ttatttcttc
     5101 gttcacattc tcctgtgccc gaaatatagc agggagaagc agaaaacaca ttatgaggag
     5161 atgggtcttt ttcaatagaa tttatattta caaaatctca tattttttaa ggatttttgc
     5221 ctaagacgaa cgaagctgtc agtccggaag ttatacccag ccggggatgg gtcgagaatg
     5281 cgatatcgac tgaagcaggg cctgtcagaa atccgatacc aaatgtaaat gagggctctg
     5341 cagtgctgta tccgcagcgc agtatagctt ttttaccggt tgcatattcg aagccgcttc
     5401 tgaatacagg ctttctgccc gataccagtt cagcttcaaa acctgcaaag aggccttctg
     5461 aaattgtcat tcccgctccg gctctaattg atgaaggcat cggaatattt cttagagaat
     5521 ttggtatcgg gttgaaaata tgaataccta cccttatatc gcgcgatggt tcatatatca
     5581 gtcccgcttc gcacgaaacc tgcaggtagt ctttgtattc cccctgctgg tgttctgcaa
     5641 aaaggtcaat ctgcactccg gccgaaaggt cggaagccag tttcagtccg ctcgccatgg
     5701 caatggtttc tctccgatat cctgtaaacc ctgaacggga ataaaaaatg ccggccgatc
     5761 cgcttccggc aggaattatc aggcaggccg attttactgc cagttctttt atagcaaagc
     5821 ggttttcgta gctgaacgag aatgctgcat tgtggtaata cgacagtgag gcctgattgt
     5881 gaaatgaaga ccatagcccc ggcttcatga tacacacata ggccataccg gcttctcctg
     5941 ctcccgcagc aggacggtaa aaaccatcag ctgtggcaac tgcagacagt aaaagcaaaa
     6001 ccagtgaaac agtaattttt tgcagcgaga ccaactgttt cgttaattgt attgtaaaaa
     6061 tataaaaagt ccgaagaata aaaataattt aattgcaaaa atttattacc gcctttaata
     6121 tattgtaaaa cataattttt ttgtacatct attatataga taaacaaatt tttatataca
     6181 ttaacaatta aacaggaagg ttttcaaaat aatacgacag attatgcttg ttaatttcaa
     6241 gcagcttttt aaaattgtta aaaactttac tggttatgag ataaatttaa tttatttaaa
     6301 agataaattt gtaaagcaat gagctttctg ggtatggaaa ttaaaacaga tctatatttg
     6361 ggtgattgca aagagaagtt gaagcttatt cctgataatt cagttgattt aatttttact
     6421 tcacccccat atgctgatca gaggcgtaat acgtatggtg gtattcatcc agataagtat
     6481 gtagaatggt ttttacctat ttcattacaa ttatatagag ttctaaagcc aactggcact
     6541 tttgttctta atattaagga aaaagttgtt aacggtgaaa gaagcacata tgtgattgaa
     6601 ctcatacttg aaatgaaaaa acaaggatgg ctatggacag aagagtttat ctggcacaag
     6661 aagaatagtt atcctgggaa atggcctaat aggttcagag acgcatggga acgtctttta
     6721 caattcaata aaagtcgtaa atttaacatg tatcaagatc aggttatgat cccaatgggt
     6781 gaatgggcta gaactagatt aagaaaactt tcagaaactg ataaaattag ggacaattca
     6841 aaagtgggaa gcgggtttgg taaaaaaatc tcaaattggc ttaataggga aaaagtttat
     6901 ccaacaaatg ttttacatct ggctactgaa tgtagtaata aaaaccatag tgcagctttc
     6961 cccgaggagt taccagagtg gtttattaaa ttatttacaa aagaatacga tattgttctt
     7021 gacccgttca tgggatctgg aacaacatta attgttgcta acagaatgag gagacactca
     7081 attggaattg atattgttcc agaatatttt cagatggtta aaaagcaatt gaagcctgtc
     7141 gaattgatat tattagaacc tgaatctgaa atttataatg agaaaattaa accttaaaga
     7201 cgttcttgag tacgttgaaa aaaatataga aacatttcat agtaaacgaa ttcaaagtct
     7261 tgatagttta aggcttacgc aagttctaaa acgaaagaat ccatatcttt ttaaagctaa
     7321 aaatgtcttg acagctgagc aaattgttaa aggcatagtt gatgctcata tatcttcaaa
     7381 tgaagaaaca atatttgggg aatggcttga aggattggct atttttataa ataaaaaggt
     7441 ttttggtggt tataaatctg gaattaaagg tatcgatctt gagtttgata aaaatgggat
     7501 taggtacatt gttactatta aatcaggtcc taattggggg aatagttcac aaattgccaa
     7561 aatggtcgaa gatttcagaa cggcaaagag aacattaaga actaacaatt ctaatcttaa
     7621 tattgtagca gttaatggtt gttgttatgg aagagacaat agacctgata agggggatta
     7681 ttttaaatat tgtggtcaga aattttggga gtttatatca aataataaaa atttatatac
     7741 tgatatcgtt gaaccattgg gatataaagc aaaagaaaaa aatgatgaat ataacaaggt
     7801 atattcccaa atgataaata aatttactaa agaattcgtt ctgaattttt gtaaagcaaa
     7861 tggtgaaata gattgggaga aactggttcg attcaactct gcagaaaatg tttaaaagtt
     7921 tgttaatgtt attttagtaa tcttctggag gataataaaa aacagtatcg tatattctca
     7981 tattatcagt ttgttttata tggatatttg acgtttttca gttcttcatt tgcctgaaca
     8041 actttcttct tgaggccttc cttgaatttt ttaatttcct ctgcaacttc gggttcacct
     8101 gtggcaataa tctgtgctgc aagtataccg gcattctgtg ctgcattgat tcctacagta
     8161 gctactggca cccccgatgg catctgtagt attgaaagaa ttgaatcgag cccttcaagc
     8221 gatgctttta tcggaacacc tatcactggc agggtagtca tggctgcaat aactccgggc
     8281 aggtgggcag ccataccggc tcctgcaata attaccttta tccccctcga tgcggcattc
     8341 cgggcaaatt tctcaacttc ctcaggagtt ctgtgtgccg agagggcata tatctcaaaa
     8401 ggtatcctga aatcgttcag aatttttgca gctccctcca ttacaggcat gtcagatgtg
     8461 cttcccatta tgatgcttac ttttggctcc atatctttaa ttttattttg ttattgaccg
     8521 aaagcaaaag taattattat tttctatttt cgcagtggca aacagagaac atggctgatg
     8581 aaaaaaatca atattctcgg aaaagaattc atcgagctga ttcctgcagg ggcgattgaa
     8641 aaaagaatag aggaaatggc aggggaaatt aacagggatt atcgagggaa agaagttatt
     8701 tttattggaa ttctgaacgg agcatttctg tttgccgcaa gtcttttcaa gaaaattacg
     8761 ctggatgctc atattacttt tgttaaactt gcttcataca ggggaacacg ttcttcgggt
     8821 gaagtgaaag aattgattgg atggaatgaa gatatcagcg ggaaggatgt tattgttctt
     8881 gaggatattg tggatacggg agtcactctt gaaagggtta tcgatgaaat aaaaataaga
     8941 aaagcaggtt cagtgaaagt tgcaaccctt ttactcaagc ctgaagcctg caaaaaggac
     9001 gtccaggttg attatgtagg cttcaaaatc ccgaacaaat acgttgttgg atacggactt
     9061 gattatgagg gttatgcgcg aaacctgcca tctatataca aaatggtcga ataataaaac
     9121 tgttttaaaa agctattaaa aactatggtg aactttttaa tttttggtcc tccgggttca
     9181 ggaaaaggaa cccagtcggt gaggcttgcc gaaaaattta atctgctgca tctttcaacc
     9241 ggcgacatgc tcagggctga gatagctgct ggtacagaac tcgggaaacg aatgagtgca
     9301 attatggcaa aaggtgaact ggtaccggat gaggttgtta tcgaaatgat tgcctcaaag
     9361 attgatggtg caaaaggtta tgccggattt ctgtacgacg gattccccag aacagtagcc
     9421 caggcagttg cactggaaaa gatgctcaac cagagaggaa tgaaaatcga ctgtatgctg
     9481 gtgctggagg ttgacaggga tgaacttgtc aaaaggcttg tcttaagagc agaacagtcg
     9541 ggcagacctg atgacaaaga ccctgccgtg attgacaaca ggattgaagt ttacagggaa
     9601 aaaacagaac cgataataag ttatttcaag gaaaaaggta tatatcagcc ggttaacgga
     9661 gtgggagaaa ttgaagatat tttccacagg ctttcagata tcgtggtaaa atatctgaat
     9721 tagaagcccg tataatttat tttccccttg ctggtttata aaaggcaaaa gctatggctg
     9781 aatctaattt tgttgattat gtaaagattt tttgccgttc cggcagggga ggtgcaggtt
     9841 ctgcacattt tcgcagggag aagtttgtac ccaaaggcgg acccgatgga ggtgatggag
     9901 gaaggggcgg acatatcata ctcaggggca atgaacagct atggacgctt cttcacctga
     9961 agtatcaccg ccattttttt gccggaaacg gtgaacctgg aggaaggcag cagagcacag
    10021 gtgcctcagg aaaggatgtg gtgatagaag ttcctcttgg cactgttgta agaaacgccg
    10081 atacaggaga gtttcttttc gagattacaa gacataatga ggaaaagata cttcttcaag
    10141 gcggaagggg aggactggga aatgttcatt tcaaatcccc gacaaaccag acaccccgtt
    10201 atgctcaacc cggagaaccc ggagccgaag caaccgtaat acttgaactt aaaatccttg
    10261 ccgacgtcgg acttgtggga tttccaaatg ccggaaaatc aacactgctt tcagtggtaa
    10321 ctgctgcaaa accaaaaata gccgactatc cttttactac tctggaacca aacctcggaa
    10381 tagtaagcta cagagacggt agatctttcg tgatggctga tatacccggt ataatcgaag
    10441 gagcccacga aggcaaaggc cttggtctga gattcctgcg acacatagaa agaaattcag
    10501 tgcttctgtt catggtgccg gtcgattcgg taaatattaa ggcagaatac gaaatactcc
    10561 tgaatgaact gcggtgttat aaccctgaac tgctggataa aaaaaaggtt ctggcaatta
    10621 caaagtcgga tcttatagat gaagaaatca aaaaggagat tagcaaaaac ctgcccgata
    10681 tcccatgggt cttcatctct tccgttacag gtcagggcat cgatacattg aaagatttgt
    10741 tatggaaagt acttaataat tagtaatatg cttgaagcgc tcgatcgcca gcttttcctc
    10801 tttctcaatt ctctcaactc tcccttctgg gaccatgtga tgttcataat aagtgctaaa
    10861 cttacatggg tgcctcttta ccttgctatc atctatgcat tgtggcgtaa atacaaaagg
    10921 aaacttattg taatactgct gtttgtaatt ctcgcagtaa cactcagcga caggctttcg
    10981 gtagcagtaa agaatactgt gaaaaggccc aggccatgcc atgagaaatc cctggaagga
    11041 atggttcata ctgtgaaggg taaatgcggt ggacaatatg gttttgtatc atcccatgcc
    11101 tccaactcat ttatggtggc tctcttcagc ctgctgctca taagaaaaag atggtatact
    11161 gtatcaataa ttttatgggc acttgtggtg ggttacagca gaatatacct tggagttcat
    11221 tacccgggag atgtggttgc aggctcagca ctgggtgctc ttgccggttg gtttacaata
    11281 tttctgtata acttaactga cagcagattt cttcagaaat ccggatattt tagccagaat
    11341 aaaataaaca aggcttaatg tcaggtactg ttttggaaac atacgatccc ttctttaatc
    11401 ttgcagttga ggattatctt cttcataaca gtaataagga atattgcctt atatatataa
    11461 atgatccatc agtagttgta ggcaggcacc aggtcattta tcgtgaaaca aactatttta
    11521 ctgccgaaaa actcaacatt cctgttataa ggagaatctc cggtggtggt actgtgtacc
    11581 acgacagagg gaaccttaat tactcgttta taattaattc agcaaaaggg atgcaggtgg
    11641 atttcgtaag gcatacaagg ccggtaattg agtttctaaa cataaatgga atacctgcag
    11701 tacttagtgg taaaagcgac attacagtta atgggtttaa agtgtcgggt aatgctgaac
    11761 acgttttcag ggaaagggta ttacaccatg gaaccttgct tttcgatgca tcactcgaaa
    11821 caatgaacag tttgctgaga ggtgatgctg taggatattc cacaaaagca attggttcta
    11881 acagaatgca ggtcatgaat ctgaaaggca ggataatcaa tattgaaacc accgaagaac
    11941 ttgctattaa attttttgaa cattcactac aaggttcaag tgttacataa attgttcca
//
LOCUS       DAOSNB010000038        11941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5311_length_11941_cov_4.346795, whole genome shotgun sequence.
ACCESSION   DAOSNB010000038 DAOSNB010000000
VERSION     DAOSNB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5311_length_11941_cov_4.346795"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PLG42_02980"
     CDS             <1..269
                     /locus_tag="PLG42_02980"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU30208.1"
                     /translation="HCLIKLDTISSNFWKTGRFTDNLVNKNPRFIDENAYDFRPDTLS
                     PLINTGNKQVVNIYNEDYRGTTRLTDGLPDIGAFERIPGEKKLN"
     gene            282..1331
                     /locus_tag="PLG42_02985"
     CDS             282..1331
                     /locus_tag="PLG42_02985"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOU30209.1"
                     /translation="MLYRILLRILLLIICIVSNFKVVTSQENARRKYRFMFYNVENLF
                     DTENDPATEDDEFLPGGERRWNANRYRQKLQSIYKVITAAGQWDPPEIIGICEVENKK
                     VIDDLLTLTPLNVQSIYDIVHEDSDDPRGIDVCLIYRKDRIKLLYYKYIYPHSLYYQK
                     IRTRKILYTKFLVEDDTLHLFLNHWPSRRGGVLAGEGSRIALANLLRQQSDSVRNSSG
                     ECSKIIFAGDFNSLPSSDEIKVLTASGEKGSFLNLGMSHFLRGTGTYKYEGIWEMPDQ
                     IIVSKTLLPDFQRCKGIYTSEDFFEIFSPDFLLLRDRKFSGQKPFSTYSGYSYQGGFS
                     DHLPVILDLVVQPGR"
     gene            complement(1279..2742)
                     /locus_tag="PLG42_02990"
     CDS             complement(1279..2742)
                     /locus_tag="PLG42_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008019472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU30210.1"
                     /translation="MTICLEDKIFEVISEVLESENVEGYVVGGYVRDFLLGRKNPEQD
                     IDIVVTGDGIDIARKTARKLGKGIKVAVYKNFGTAMFRYGDYNIEFVRARKESYRRDS
                     RKPFVESGTLEDDQKRRDFTINALAAGLNRSTWGVLVDPFGGKEDLKKRLIRTPLDPD
                     RTFDDDPLRMMRAIRFATQLDFKIEEKTFESIKKNRERIKIVSTERITTELNKIISSP
                     KPSKGFMLLDMSGLLEIIFPELANLKGVEKKEGRGHKDNFLHTLKVLDNVSSKSDNLW
                     LRWAALLHDIAKPVTKKYSEKTGWTFHNHEFIGAKMVPVIFRKLKLPLNENMKYVRKL
                     VDLHLRPIVLSQDIVTDSAVRRLLYEAGDDIDDLMLLCEADITSKNEEKKSRHLANFR
                     LVRKKLVEIEEKDAIRNFQPPVSGDEIIATFGIKPGKEVGIIKNYIKDAILDGIIPNE
                     YNAARELMIKKGLELGLKPLVSGRAAQPDQELREDGR"
     gene            2896..3276
                     /gene="trxA"
                     /locus_tag="PLG42_02995"
     CDS             2896..3276
                     /gene="trxA"
                     /locus_tag="PLG42_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU30211.1"
                     /translation="MIEHLTKETFLKKVFNYEKNKEWKFEGERPCVVDFYADWCGPCK
                     MVAPILEQLSKEYDGKIDFYKVDTEKEDELAAVFGIQNIPSLLFVPKDGQPQMALGAL
                     PKETFEMAFKEVFGIEKEKNEKNQ"
     gene            complement(3417..3614)
                     /gene="xseB"
                     /locus_tag="PLG42_03000"
     CDS             complement(3417..3614)
                     /gene="xseB"
                     /locus_tag="PLG42_03000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOU30212.1"
                     /translation="MAKKELSFNEAMKEIEAILRNIESGNLDIDRLSKEVKRASDLIR
                     LCQKKLRATEEEINGIFKELS"
     gene            complement(3642..5027)
                     /gene="xseA"
                     /locus_tag="PLG42_03005"
     CDS             complement(3642..5027)
                     /gene="xseA"
                     /locus_tag="PLG42_03005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOU30213.1"
                     /translation="MNEKLSLTELQQVIRDSLYAALPGFYWVFAEISEAKENYAGHCY
                     LELIEKHPDEINIRARVRAVIWNTRYRFLKSFFESTTGETLRPGIKILVRVKIEYHEV
                     YGLSLVINDIDPAFTLGDMAIKRQQILRRLEEEGVITMNKELELTIAPQRIAVVSSKT
                     AAGYQDFVRHLKENIYGYGFSITLFEAVMQGAETEQSVISALDRISEQYEKFDVAVII
                     RGGGAQSDLSWFDNYNIAYHITQFPLPVITGIGHEKDISVTDIVAYHALKTPTAVADY
                     LIEKMVSAENLLMQLADEISEAVNEIIDSKKERLTSFMTRIIPVSNRLITSVRKDLSV
                     KLVDLVTNVRNRLGREEIFLADVKKKIESASRNYTSSRERDLSRAGELIRINTINLLK
                     SIEVRIKSMSESLNILSPDNVLKRGYTITVLNGQIVSSADKIKPEDEIDTYFHDGKIK
                     SRVLKKSKEKL"
     gene            complement(5030..6700)
                     /locus_tag="PLG42_03010"
     CDS             complement(5030..6700)
                     /locus_tag="PLG42_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOU30214.1"
                     /translation="MVNKPVKKIGYYLAVAFLILYFSSGHTSIAQEPDYRYFYRVYFT
                     DKGDYKPENFSPSRLLSPKAIARRIKYGIKFPDYTDLPVYPPYINQIRSLGLTLHNTS
                     KWMNTALFKSITPAPVSVIESLPFVEGVRVVKAPSAKSVKPDKLELIQGVNNEVPYDR
                     QITMLEGNFLHDSGFDGTGVVIAVLDAGFSRANFLPCLDKLRARYGIRGTYDFVLNSK
                     NVYSYHYHGTAVLSVLAGDVPGKIVGSAPGANYWLFRTEDVYSEFPVEEDYWAAAAEY
                     ADSLGADIISTSLGYYVFDDPSMNYKFEDLNGMNAFITRVADIAVSKGIIVVASAGNE
                     RDNEWLRIIAPSDGFKVICVGAVDQYCAISSFSSAGPSADGRIKPDNAAMGVDVTAQI
                     DEKGYIKISGTSFSCPILSGMCACLLQAVPEASPSDIIDILHRCSDRANNPDSLYGFG
                     IPSMKKAIQMLQEKIMLKPGIDITAGPNPFTDHIDIYFREPPYSPLSVEIYSSTGHLL
                     YKKIITSRDYISKTLRLNDLQIRNQGIYFLKIMTDNGTSVMKVVKIND"
     gene            complement(6694..7503)
                     /locus_tag="PLG42_03015"
     CDS             complement(6694..7503)
                     /locus_tag="PLG42_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOU30215.1"
                     /translation="MALMKTKMRILSLIVILLITGSTVALSQPNDTINRFDKTGKRQG
                     YWMKRYPNGHIQYEGYFKDDRPVKVFKRYYDNDTLQSILFYSDDSRTADAIFFHPNGF
                     IAASGKYVNQKKEGKWEFFSSAIKGYKITDEEYSNNMKNGLSVKYYPDKTVAEKIWYR
                     DNIKDGEWIQYHENGKIFLKAQYVRGVLEGPFEVWDANGNLKYKGQYKNDRREGIWYV
                     YNPDGTVKNEIKYINGMATNPELYIRENAILDSLEVTGKNLSDPEKTGTIW"
     gene            complement(7565..9475)
                     /gene="dnaK"
                     /locus_tag="PLG42_03020"
     CDS             complement(7565..9475)
                     /gene="dnaK"
                     /locus_tag="PLG42_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOU30216.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGKRTTPSVVSFAE
                     NGERKVGDPAKRQAIINAKRTVYSIKRFMGETYDKVQKEIERVTYPVVRGENNTPRVK
                     IDDRLYSPQEISAMILQKMKKTAEDYLGYEVTDAVITVPAYFSDSQRQATKEAGEIAG
                     LNVRRIINEPTAASLAYGLDKKAQDMKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHLIIDWLAEEFLKENGVDLRKDPMAMQRLKEAAEKAKIELSSSTTTEIN
                     LPYIMPVNGIPKHLVKTLTRAQFEKICDKLIQAVIPPCEKALEAAGLKPSDIDEVILV
                     GGSTRIPAIQQVVEKFFGRTPSKGVNPDEVVAIGAAIQGGVLAGEVKDVLLLDVTPLS
                     LGIETLGGVMTKLIEANTTIPTRKTEVFTTAADNQSSVEIHVLQGERPMAAGNKTIGR
                     FYLDGIPPAPRGVPQIEVTFDIDANGILHVSAKDRGTGKEQKIRIEASSGLTEEEIRR
                     MKEEAKANEEADRRARERADKLNAADSMIFQTEKQLREFGDKLSPDKRREIEQALSKL
                     KDAHKSQDIPAVDKALADLNNAWQKASEEMYRAAQASGAGAAGPGPGASTGSSGKSGN
                     EEVTDVDFEEVK"
     gene            9672..10487
                     /gene="panB"
                     /locus_tag="PLG42_03025"
     CDS             9672..10487
                     /gene="panB"
                     /locus_tag="PLG42_03025"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOU30217.1"
                     /translation="MSEFKAVKRVTTHVLFEMKLRGEKIAMLTAYDYSFARILDEAGI
                     DIILVGDSASNVMAGYETTLPITLDNMIYHASSVVRGVKRALVVVDMPFGTYQGNSRE
                     ALASAIRIMKETGAHAVKLEGGAEVAESVERIISAGIPVMGHLGLTPQSIHKFGTYVV
                     RAREEEEARKLLEDAKLLQNLGIFSLVLEKIPAKLAGEVTSSLKIPVIGIGAGPNTDG
                     QVLVLHDMLGITQEFSPRFLRRYHNLNAEIKGAVQNYINDVKTLKFPNEKEQY"
     gene            10496..11203
                     /locus_tag="PLG42_03030"
     CDS             10496..11203
                     /locus_tag="PLG42_03030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOU30218.1"
                     /translation="MAEHDSDKLKILYEDNHLIAVYKEASELSQGDRTGDITLDEKVK
                     RYIAKKYNKPGDVFLGIVHRLDRPVSGVMLYARTSKALKRLNRMFQEREVQKIYWAVT
                     GDRPPADEATIEHFIKKNEKQNKSYVYDHEVKGSRKASLTYRLIGRSQRYYLIEVQLH
                     TGRHHQIRAQLAAIGCPIKGDLKYGAKRSNEDGSISLHARSLAFKHPVTKEEIRIIAP
                     LPENGIWKIFKNFDSKK"
     gene            11393..>11941
                     /locus_tag="PLG42_03035"
     CDS             11393..>11941
                     /locus_tag="PLG42_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30219.1"
                     /translation="MKNKIFISLVVMMALSVIGIFYVQITWMRNAIRTSNENFNNAVF
                     IGLNNAASEIESYHRMNFFNNYIFRDPFSFRDTVTGLSEFFSFNGFFSDNKGSVTINF
                     NSRQASGSINPESRNRTNDNARIASRQNQDSIYIITRGDRPGNVRIERKNDLTGAPGN
                     SVIMTQSDFLQWLRKRTNEFQQL"
BASE COUNT         3259 a         2537 c         2441 g         3704 t
ORIGIN      
        1 accattgtct gataaaactt gataccattt cttctaattt ctggaaaacc ggcagattta
       61 ctgataatct tgtgaacaaa aacccgcgtt ttattgatga aaacgcttat gatttcagac
      121 ccgacaccct ttctcctttg ataaataccg ggaacaagca ggttgtgaat atttacaatg
      181 aggactacag aggtacgaca cgactcacag acggattgcc cgacataggc gcttttgaac
      241 gcattcccgg cgaaaagaaa cttaactgac agcttctgat tatgctctac agaatattac
      301 ttagaatatt gttgttaata atctgcattg tatcaaattt taaagtggtt acatcccagg
      361 aaaatgcccg gaggaaatac cggttcatgt tttacaatgt tgaaaatctt tttgatacag
      421 aaaatgatcc tgcaaccgaa gatgatgaat ttttacccgg gggagaacgg cggtggaacg
      481 cgaacaggta caggcaaaaa ttacaatcta tatataaagt tatcacagca gccggacagt
      541 gggaccctcc tgaaattatt ggaatttgcg aggtagaaaa caaaaaagtc attgacgacc
      601 tgcttacgtt aacccctttg aatgttcaga gcatttatga tatcgtacat gaagattctg
      661 atgatccgag gggcattgat gtttgtttga tttacaggaa ggatagaatt aagttattgt
      721 attacaaata catatatcca cattcgctct attatcagaa aataagaaca aggaaaatat
      781 tatatactaa atttttagtt gaagatgata cccttcatct ttttttgaat cactggccat
      841 cgagaagggg aggggtgctt gcaggcgagg gtagcaggat cgctctggca aatttgctca
      901 ggcaacagtc cgattcagtt agaaattcat caggggaatg ttcaaaaatt atttttgccg
      961 gtgattttaa ttctcttcct tcatctgatg aaattaaggt tctcacagct tctggagaaa
     1021 agggctcttt tttgaatctt ggcatgagcc attttcttcg tggtacaggc acttataaat
     1081 atgagggaat atgggaaatg cccgatcaga taatagtttc gaaaaccctg cttcctgatt
     1141 ttcagagatg caaaggtatt tacacttctg aagatttctt tgaaatattt tctccagatt
     1201 ttctgcttct cagggacagg aaattttccg ggcagaagcc attttcaacc tacagtggtt
     1261 attcttatca gggaggattc agcgaccatc ttcccgtaat tcttgatctg gttgtgcagc
     1321 ccggccgctg accaaaggtt taagtcctaa ctcaagacct ttcttaatca tcagttccct
     1381 tgcagcatta tattcattgg ggataattcc atcaagaatt gcatctttga tatagttctt
     1441 aataatgcca acctctttgc ctggttttat accgaacgtt gcaattattt catccccgga
     1501 aactggcggt tgaaaatttc taattgcgtc tttttcttca atttcaacaa gtttctttct
     1561 taccagtctg aagtttgcaa gatgccgtga tttcttttcc tcatttttgg aggtgatatc
     1621 agcttcacac agaagcatta gatcatctat atcatcacct gcttcgtaaa gaagtcttct
     1681 taccgcagaa tcggtgacaa tatcctgtga cagcacgatg ggacgcaggt ggaggtcaac
     1741 aagtttccgt acatatttca tgttttcgtt cagaggaagc ttcaactttc tgaaaatgac
     1801 aggcaccatc tttgccccta taaattcatg gttatgaaac gtccagccag ttttttcaga
     1861 atatttcttc gttacgggtt tggcaatgtc gtgaagaagt gctgcccacc tgagccacag
     1921 attatccgat ttgctgctta cattatcgag cactttaagg gtatgaagaa aattgtcttt
     1981 atgtcccctt ccttctttct tttccacccc tttcagattt gcaagttcag gaaagataat
     2041 ttccagcaga cccgacatat cgagcagcat aaaccctttt gatggctttg gagatgaaat
     2101 tatcttgttt aactctgtag ttattctttc ggtcgaaact atcttaatac gctccctgtt
     2161 cttttttatt gattcaaaag ttttctcttc aattttgaaa tcgagctggg tggcaaatct
     2221 tatcgccctc atcatcctga gagggtcatc atcgaatgtt ctgtcaggat caagaggagt
     2281 tcttatcaac cttttcttta aatcttcctt ccctccgaaa ggatcaacaa gcacacccca
     2341 cgttgaacgg ttcaatcctg cagcgagggc attaatcgta aagtccctgc gtttctggtc
     2401 gtcttctaaa gtgccgcttt caacaaaagg ctttcgagaa tcccttctgt acgattcttt
     2461 tcgtgccctt acaaattcaa tattgtaatc cccataccgg aacattgctg ttccgaaatt
     2521 cttgtaaaca gcaaccttaa ttcctttccc cagttttctt gcagttttcc tggctatgtc
     2581 aatcccgtca cccgtcacaa caatatcaat atcctgctct ggatttttac ggccgaggag
     2641 aaaatccctt acataacccc ccacaacata accttcaaca ttttcagatt caagtacctc
     2701 tgaaataact tcaaagattt tatcttccag acagattgtc attatttctt caaaatgtga
     2761 tgctttcgtg gcaaaattac caatttatct ttttattttc ggcaggatag tgatttatta
     2821 aagtctgtgg catgataatt gacatatcaa tgaacaaata ataagattac ttgttaacca
     2881 taaaaagttt ataacatgat tgaacattta acaaaagaga cttttcttaa gaaagtattt
     2941 aactacgaaa aaaacaaaga atggaaattt gaaggtgaga ggccttgtgt ggttgatttc
     3001 tatgccgatt ggtgcggacc ctgtaaaatg gtagcaccta tacttgagca actttcgaag
     3061 gagtacgacg gaaagattga tttctacaag gttgatactg aaaaggagga tgaacttgct
     3121 gctgtttttg gaatccagaa catacccagc cttctgtttg tccctaaaga tggacaacct
     3181 caaatggcat taggtgccct tccgaaagag actttcgaaa tggctttcaa ggaagtattt
     3241 ggcatagaga aggaaaaaaa tgaaaaaaat cagtaaaaag tgaaactttt tacctgtttt
     3301 ttacgttata attattgaaa caacacacct ctcaagggtt aagttttttc atagggttta
     3361 ggttagggta tttaaagggt ttcccgcagg agaaaaagtt ctgcgggatc tcttttttat
     3421 gaaagctctt tgaatatgcc gtttatctct tcttctgtgg cccttaactt tttctgacac
     3481 agccttataa gatctgaagc cctctttact tcttttgata acctgtcaat atcaagatta
     3541 ccgctttcaa tattgcgaag aatggcctca atttctttca ttgcttcgtt aaatgacagc
     3601 tcttttttag ccatttctct gaattttact taattacaaa tctataattt ttcttttgat
     3661 tttttcagta ccctgctctt tatttttcca tcgtggaaat atgtgtcaat ttcatcttcc
     3721 ggttttattt tatctgcact gctgacaatc tgaccgttaa gtactgtaat ggtgtatccg
     3781 cgttttagaa cattatccgg acttaatatg ttaagagatt cagacatcga ttttattctg
     3841 acttcgatgc tttttaacag attgattgtg tttatcctta taagttcacc tgccctgctg
     3901 aggtccctct ccctgctgga agtataattc cgggatgctg attcaatttt ctttttcaca
     3961 tctgccagaa atatctcttc tctcccaagc ctgttcctga cattggttac aagatcaaca
     4021 agtttaactg ataaatcctt tcttactgaa gttataagcc tgttagaaac aggtattatt
     4081 cttgtcatga aagatgtcag acgctctttc ttactgtcaa tgatttcgtt tactgcttct
     4141 gatatttcat cggccagctg cataagcagg ttttcggccg agaccatttt ttctataagg
     4201 tagtcagcca cagccgttgg tgttttaagt gcatgatatg cgacaatatc tgttacggag
     4261 atatcttttt cgtggccaat tcctgttata acaggcagtg gaaactgtgt gatatggtag
     4321 gctatattgt agttgtcgaa ccagctcagg tccgattggg caccgccgcc ccttataatt
     4381 acagccacat caaatttctc gtactgctca gatatacggt caagtgctga aatcacgctt
     4441 tgctcagttt cggcgccctg catcactgct tcaaaaagtg ttatgctgaa accgtatccg
     4501 taaatatttt ccttcaggtg cctcacaaaa tcctggtatc cggcagcagt ttttgacgaa
     4561 accactgcaa tacgttgtgg tgcaatcgta agttcaagtt ctttgttcat cgttatcacc
     4621 ccttcttcct caagccttct cagaatctgc tgtcgtttaa ttgccatatc gccgagggtg
     4681 aaagcaggat cgatatcgtt tattacaaga ctcaatccgt aaacctcatg atattctatt
     4741 ttgaccctta ccagaatttt aatacccgga cgaagggttt ctcccgttgt gctttcaaaa
     4801 aatgatttca gaaaccggta ccttgtgttc cagattacag ccctaactct tgccctgata
     4861 ttaatttcgt cggggtgttt ttcaattagt tcgagatagc aatgtccggc atagttttcc
     4921 tttgcctcgg aaatttctgc aaaaacccag taaaaacctg gaagtgccgc atatagtgaa
     4981 tccctgataa cctgttgaag ttcggtgagt gacagttttt cgttcattat cagtcattta
     5041 tttttacaac tttcataaca gatgtcccat tatctgtcat gatttttaaa aagtaaattc
     5101 cctggttcct gatttgtaaa tcgttcaggc gaagagtttt gctgatatag tctctgctgg
     5161 ttattatttt tttataaaga agatgaccgg tagatgaata gatttctacg cttagagggg
     5221 aatatggtgg ttccctgaag tagatatcaa tatggtcggt aaatggatta ggtcctgcag
     5281 taatgtctat gccaggtttc agcataatct tttcctggag catctggatt gctttcttca
     5341 tactgggaat gccgaaaccg tacagggagt cgggattatt tgccctgtcg ctgcaccggt
     5401 ggagaatatc tattatatct gaaggtgaag cttcaggtac tgcctgaagt aagcatgcac
     5461 acattccact tagaatcggg cacgaaaaag atgtaccgct gatcttgatg tacccttttt
     5521 catctatctg ggcagttaca tcaactccca ttgccgcatt gtcaggtttt atcctgccgt
     5581 cggcacttgg tccagccgac gaaaaggaag aaatcgcaca gtattgatct actgccccta
     5641 cacagataac tttaaacccg tctgatggag caattattct caaccattca ttatcccttt
     5701 cgttccctgc gcttgcaaca acaataatac ctttcgatac ggcaatatcc gctacacggg
     5761 taatgaaagc attcattccg ttcaggtctt cgaatttgta attcatggaa ggatcatcaa
     5821 aaacataata tccaagggaa gttgaaataa tgtcagcacc cagactgtcg gcatattctg
     5881 cagctgctgc ccagtagtct tcttccactg ggaactcaga atatacatct tcagttctga
     5941 agagccagta attagccccg ggagccgaac ccactatttt ccctggtaca tcacctgcca
     6001 gtactgacaa tacggcagtg ccgtggtaat ggtaactgta aacatttttg gagttcagca
     6061 caaagtcata agttcctctt atgccataac gtgcccgtaa tttatcaagg cagggtaaaa
     6121 aatttgccct ggagaagcct gcgtcgagaa cagctatcac cacgccggtt ccgtcaaatc
     6181 cggaatcatg cagaaaatta ccttcgagca ttgtaatctg tctgtcatag ggaacttcat
     6241 tgtttacccc ctggatcagt tccagtttat ctggcttaac gcttttagct gaaggtgcct
     6301 tgacaaccct gactccttca acaaagggaa ggctttctat aacggatacc ggtgcaggtg
     6361 taatggattt aaagagagct gtattcatcc acttcgaagt gttgtgaagt gtcaggccaa
     6421 gagatcttat ctgattgatg tacggcgggt aaacgggaag gtcagtataa tccggaaatt
     6481 ttatgccgta ttttatacgt cgggcaattg cttttggact gagcagccgt gaaggggaaa
     6541 aattttcagg tttataatcg cctttatcag tgaaatatac cctgtagaaa tatctgtaat
     6601 ctggctcctg tgctattgat gtgtgccctg aagaaaagta aagaatcaga aacgcaactg
     6661 cgagataata tccaatcttt tttaccggtt tatttaccat atagttccgg ttttctccgg
     6721 gtcggagagg tttttgcctg taacttcaag tgagtcgaga atagcatttt ctcttatgta
     6781 aagctccggg ttggtagcca tcccatttat atactttatt tcgtttttaa cagttccgtc
     6841 ggggttatag acgtaccata tcccttctcg tctgtcgttt ttatattgcc ccttgtactt
     6901 gaggttccca ttggcatccc acacttcaaa agggccttcg aggacgcctc ttacatattg
     6961 agcttttaga aaaattttcc cattttcatg atactgaatc cattctccat ctttaatatt
     7021 atctcggtac catatttttt ccgccaccgt tttatcgggg taatatttta ccgagagtcc
     7081 gtttttcatg ttattgctgt attcttcatc cgttatttta tagcctttga ttgcagatga
     7141 aaagaactcc cattttcctt ctttcttctg gtttacatat ttccctgaag ctgctataaa
     7201 accgtttgga tggaaaaata ttgcatctgc tgtgcggctg tcatcgctgt aaaaaagtat
     7261 tgattgcagg gtgtcgttat cgtaatatct cttgaatact ttaaccggac ggtcgtcctt
     7321 gaaatagcct tcgtactgga tatggccatt agggtatctt ttcatccagt aaccctgcct
     7381 tttccccgtt ttatcaaaac ggtttatggt gtcgtttggc tgggacaggg caactgttga
     7441 accggttatt aggagaataa caatcaggga aagtattctc attttggttt tcatcagagc
     7501 cataaagata aaaaaaatcc cgcacatacc tgtgcgggat gagaacgtac agctgatgag
     7561 tttattattt cacttcctcg aagtcgacat cagtaacttc ttcatttcct gatttaccgc
     7621 tgcttccggt tgatgcaccc ggacctggtc ctgcagcgcc ggcacctgaa gcctgggcag
     7681 ctctgtacat ttcttcagag gctttctgcc atgcattgtt caggtcggcg agagccttgt
     7741 caactgcagg tatgtcctgc gatttgtgag catccttgag ttttgaaaga gcctgctcaa
     7801 tttcgcgcct tttatccgga ctcagtttat caccgaattc gcgcagctgt ttttccgtct
     7861 ggaaaatcat actgtcggca gcattaagct tgtcggctct ttcacgtgcc cttctgtcgg
     7921 cttcttcgtt ggctttggct tcttccttca tccttcttat ttcttcttca gtaagaccgg
     7981 atgaagcctc aattcttatt ttctgttctt tacctgtgcc tctgtcttta gccgacacat
     8041 gaaggatacc gttggcatca atgtcgaatg ttacttctat ctgtggtaca cctctcgggg
     8101 caggtggtat gccatcgaga tagaagcgac ctattgtttt gttgcctgca gccatcggac
     8161 gttctccctg aagcacatgg atttcaaccg atgactggtt atcggcggca gtggtgaata
     8221 cctctgtctt tctggtcggt atagtggtgt tggcttcgat aagctttgtc attacgccgc
     8281 caagggtttc aataccgagc gacagcgggg tgacgtcgag aaggaggaca tcttttacct
     8341 cgcctgccag aactccaccc tgaatagcag cacctatggc aacaacctcg tcggggttaa
     8401 cacctttaga tggagtcctt ccgaagaatt tttcgacaac ctgctgaatg gcaggtatac
     8461 gtgtcgaacc tcctaccaga attacttcgt ctatatcgga aggcttcaga ccggcagctt
     8521 caagagcctt ttcacatggc ggaatgactg cctgtatcag tttgtcgcaa atcttttcaa
     8581 actgggctct tgtaagtgtc tttacaagat gctttggaat tccgttaacc ggcatgatat
     8641 acggaagatt tatttctgtg gtcgttgagc ttgaaagctc tatcttggct ttctctgcag
     8701 cttctttcag acgctgcatt gccatggggt cttttctcag atccacaccg ttttctttca
     8761 ggaattcctc tgcaagccag tcgattataa ggtggtcgaa gtcgtcaccg ccgaggtggg
     8821 tgtcaccatt tgttgattta acttcgaata ctccatcgcc aagctcgagg attgaaatat
     8881 cgaatgttcc gcctccaagg tcgaagacgg caatcttcat atcctgagcc tttttgtcga
     8941 ggccataagc cagcgaagca gctgtaggtt cgttaatgat tcggcgcacg ttaagtccgg
     9001 ctatctcacc agcctccttg gttgcctgcc tctgtgagtc gctgaaatag gctggtacag
     9061 tgatgacagc atcagtcacc tcataaccaa gataatcttc agcagtcttt ttcatcttct
     9121 gaagtatcat ggctgaaatt tcctgaggtg aatagagcct gtcatcaatt ttaacgcgcg
     9181 gagtattgtt ttcacccctt acaacaggat atgtaaccct ttcaatctct ttctgaactt
     9241 tatcatatgt ttcccccata aaccttttta tggagtaaac agttcttttc gcgttaataa
     9301 tagcctgtcg cttggctgga tccccaacct tcctttctcc attctcagca aatgaaacta
     9361 cggaaggcgt cgtccttttg ccttcactgt tgggaataac aaccggttcg ttgccttcca
     9421 taacagcaac acaggagtta gtcgtgccga ggtctattcc aattattttg cccataatat
     9481 ctttcttttt gatggttata cttttttatt gtctcttact cattaacaat gtttatgcca
     9541 atgccctgta acacggtttt tatgacaaaa agtcagagaa aatcggcaaa tgacactatt
     9601 attttaacat gaacaggttt ggctgcatta aaatttgtag ctttgtcata aaatttaaca
     9661 ctcaggaaag tatgtcagaa tttaaagctg ttaaaagggt aactacccat gttctctttg
     9721 agatgaagct caggggcgag aagattgcca tgctgacggc atatgactat tcttttgcaa
     9781 ggattctgga tgaagctggt atagatatta ttcttgttgg cgactcagct tcgaatgtga
     9841 tggccggtta cgaaactaca ttgcctatta ccctcgataa tatgatatat catgcatcgt
     9901 cggtggtaag aggtgtaaag agggcactgg tagtggttga catgcctttt ggtacgtacc
     9961 agggcaattc gagggaggct cttgcttcgg ctatacgtat aatgaaggag acaggagccc
    10021 atgccgttaa acttgaggga ggagccgaag tggctgaatc ggttgaaaga ataatttcag
    10081 cagggatacc cgttatggga catctcggac tgacaccgca gtcgatacat aaattcggaa
    10141 cctatgttgt gagggcaagg gaggaagaag aagccagaaa gctgcttgaa gatgccaaac
    10201 tgctacagaa ccttggcata ttttcccttg tgcttgagaa aatcccggct aaacttgccg
    10261 gtgaagtgac ctcatcatta aaaatacctg ttatcggcat tggtgctggc cctaataccg
    10321 acggccaggt acttgtgctc cacgatatgc tcggaataac ccaggaattt tcgccacgct
    10381 ttctcaggag gtatcataac ctcaacgctg aaataaaagg tgcagtgcag aattatatca
    10441 atgatgtgaa aacacttaag ttcccgaacg aaaaagagca gtactaacag taaagatggc
    10501 agaacacgat tcagacaaac tcaagattct ttacgaagac aaccatctga ttgctgttta
    10561 caaggaagca tcggagctct cacaggggga tcgcaccgga gatataaccc tggatgaaaa
    10621 ggtcaaaaga tacatagcca aaaagtacaa taagccgggc gatgtttttc tgggtattgt
    10681 tcacagactc gacaggcctg tgagtggagt aatgctttat gcccgtacct ccaaggcact
    10741 gaaacgatta aacagaatgt ttcaggaaag ggaggtgcaa aaaatttact gggctgtaac
    10801 cggtgatcgg ccccctgcag atgaggcaac catcgaacac tttattaaaa aaaacgaaaa
    10861 acaaaataaa tcatacgttt acgaccatga agtaaaaggc tccagaaaag cctcccttac
    10921 atatagactt attggcaggt cacaaaggta ttatcttatt gaagttcagc tccatacagg
    10981 cagacaccat cagatacgtg cacaactggc tgcaattgga tgcccgatta aaggggatct
    11041 caaatatggt gcgaaaagat caaatgaaga cggaagtata agccttcatg cccgcagcct
    11101 cgctttcaaa catccggtca caaaggaaga aatcagaatc atagcacctc ttccggaaaa
    11161 cggtatctgg aaaatattca aaaactttga ttcaaaaaaa taagccggaa cttgtttcgt
    11221 tttttccggc ttattctggg gttgtcaatg aaagccaaaa aaagtaatct gtaagtggtt
    11281 tgttgcaaaa ttaagcagca acccgggtaa ttcagttaaa atcaggttaa atgaagttaa
    11341 ggaaaagtta aagtttttac actctgagta tcaattgagt taattttgca atatgaaaaa
    11401 caaaatcttc atcagcctcg tggtgatgat ggctctctcc gtcattggaa tattttatgt
    11461 gcagattaca tggatgagaa atgccatcag gacgagcaat gagaatttca ataatgctgt
    11521 ttttatcggc ctgaacaatg ctgccagcga gattgagtca taccacagaa tgaatttttt
    11581 caataattat atattccggg atcctttttc attcagggac acggtgacgg gattatctga
    11641 atttttcagc tttaacggct tcttctcgga taataaaggt agtgttacta tcaacttcaa
    11701 cagccgtcag gccagcggaa gcattaatcc ggaaagcaga aatagaacta atgataatgc
    11761 cagaatagct tcacggcaaa atcaggattc aatatatata attacccggg gtgacaggcc
    11821 cgggaatgtc agaattgaga ggaaaaatga tctcacagga gccccgggga attcagtcat
    11881 aatgacgcag tctgattttc ttcagtggct ccggaaaaga acaaacgagt tccagcaact
    11941 g
//
LOCUS       DAOSNB010000039        11892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5335_length_11892_cov_4.508068, whole genome shotgun sequence.
ACCESSION   DAOSNB010000039 DAOSNB010000000
VERSION     DAOSNB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_11892_cov_4.508068"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1604
                     /locus_tag="PLG42_03040"
     CDS             438..1604
                     /locus_tag="PLG42_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4221 family protein"
                     /protein_id="HOU30220.1"
                     /translation="MKLIHIYFGFFLVAFYCCTEKEGKNFKDIYTLASTNQIIEIKLD
                     DNQVNLSPFIQVFNHNGGNFLAFLDFDLISINIYNIDSQIKVRSIDLKSEELKPFQSI
                     VAFYIINPDTILLVNIRPPVIVFIDSEDKIVKKIPAGNEGEFTATIPWAGQKPLLSGN
                     KLFLGQMFSFMETKGKLTGENIGSTKIAISIDLLSGLKTLYSLTYPPSLIGSDVSNMK
                     VCRTLGYNNSFIYCFSLFDSLYITNEEHVSFKSKKIETNHKFSFSREHWKYINDIQKG
                     MRDELLHDDIKNIFYDKYRECYYLLIREGQTDIDQYAPDFATRLQYGKCFIIILDKNL
                     KHLGEVYFPDNVYAFQMMFITPEGLYISRNHVRNPEYDEKYLRFECFTLRKINE"
     gene            1704..2051
                     /locus_tag="PLG42_03045"
     CDS             1704..2051
                     /locus_tag="PLG42_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30221.1"
                     /translation="MNTCKVIIIIPAEACSSCIKLSFEYIKGINSDYLLVLSSFYGKS
                     INYLIENYCLDKTKLLIDKKNMASALWLIEPTGPTIYLVRNKRIYKKYEAEKILKNPE
                     IFIEINTFLTDCK"
     gene            2339..3394
                     /locus_tag="PLG42_03050"
     CDS             2339..3394
                     /locus_tag="PLG42_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU30222.1"
                     /translation="MKVIFYSALLTGIVMFYSCKQKPSQSLEEIARRAARPEAIIVKT
                     VKAERTTFYNELISNGKIFSSKKAVIPFRVSGIIKELYIRNGQRVSEGELLAVIEDFD
                     YRVQLQRARQNLEKAEINFRDDILTNFSANDTTGLPASKIKISRIRSGLDDALTALAQ
                     AEYNYNSTRITAPVSGVVSGMEARQWNPSQNFKNLCTIADDRIMDVEFPVTESEYLML
                     RVGMPVSIIPFVNDSLKINGTITQINPQVDENGMVKVTASFANKARLIDGMNVRVLIR
                     RPVPDRIVVPKEALVRRQGRDVIFVRQDSFAIWKYVTVEAENSTSLSISEGIEPGDLV
                     IISGNINLAHETVVREQ"
     gene            3391..6582
                     /locus_tag="PLG42_03055"
     CDS             3391..6582
                     /locus_tag="PLG42_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU30223.1"
                     /translation="MSAFRINIFFVVLIITGIAITPLLSLQFNPTRYLPSLTISFSWP
                     NVPVRMVEQEVTTAIEGVLSTVSGITRIRSVTREGIGSITVEFDRRTDLQAKRFEVAS
                     LMKEAFKRLPEGVSYPSVRMNLPAIGSSGSLILSYQIIGNTSQSYIADVAEELIKPAI
                     ATIDGVYSVDVYGSTPREWEITYDLNKLSVLGLQPTTLTAAIKNYMLERELGGGHELT
                     TERSVKKTYITLAGNRTDTIKWYDIPITSISGRIVTLGDVATVRLKDRQPVSYYRING
                     LNTINLNVTAAKNVNNIKVAGEVKKTIEKIKKELPPGYSIRNSLDNTVFIRQEISKNL
                     FRTVLSVVLLLLFIFAITREFHYLLIIAISLFSNILIAFIFYYLFHLEIHLYSLAGIT
                     VSFGIIINNTIVIVDHLRHRRNMKVGISLLAATLTTIGALSVIFFLDEDMKLTLSDFA
                     AVVIINLSVSLVIALFFIPALMEKIRLKSHHTSYANCKKRAAVKFTHQYKNIIDFIIQ
                     RRTALIIMAILVFGLPVIYLPDRLPPEQSTIMQEEQLTWLQKLYNKSLGNPKYVQKFK
                     PAINKVLGGTLRLFSEKVKNSRFYYYGSSGEVQRTQITVDIGLSEPGLTVENINNICM
                     RLENMLAEYDEIDLFTTTVYSAEDATVTVTFKPEYDFTVFPYILKFKIEDFMNSIGSY
                     HTIVYGVGKAFSNQVYSDYIRGTYTINMRGYNYDELYNYCEGLKERLIKSARGRIKEV
                     FFLASSDRFNVRKNYRNTLLMDRSYLAAYDADIVSTYAGAYRLSGNSLTAGNVYTEGF
                     IAPISLKTLQAEEFDYWNFINEPILIRNGMQMKLKDFAGVVSEVSDNTISREDQEYVI
                     YVTYDFIGNYELGRMILERNVKETNSLLPLGYTAQISTYSFSWGSSKANYLLIFLVVL
                     IIWIVCSILFESLKQPLIVISLIPFSLIGVFLTFFIFNINPDEGVFAAMILLCGIVVN
                     SSIYILGDYNSLRREKPGMDERRLYLKAFNGKIIPVILTIFATIMGLIPFLLTGRDER
                     FWFNLAAGTIGGLIFSIVGLLFYQPLMLKKKGR"
     gene            6726..9773
                     /locus_tag="PLG42_03060"
     CDS             6726..9773
                     /locus_tag="PLG42_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU30224.1"
                     /translation="MVRFLITRPVAVLMTFISVIMLGIISSSRLPVSLMPDVDIPEIT
                     VAVSRPDVSAREMENTVVSILRRNLLQVPGLADLRSESHNGSALIRLSFKYGININLA
                     FMEVNEKIDGAMNNLPRDIQRPSVIKASASDIPVFMVNITLSEGYESSEKFTELSNFA
                     ETIIKKRIEQLPEVAMADITGQVKRELYILPDRKKMMSLGITEQDIIKAVELNNLNIG
                     NIMVRDGKYLLNLEFNSYLKDPEDVKNIYLNAGSARILQLKDIADVGIREEKPRGLYY
                     SNGKRAVVLAIIKEASAQMETLKSKMGELLTQFRKDYPQMDFEISQDQTLLLDYSISN
                     LRQSLWAGGLLAFFVMFFFLKDAKSPLIIGFSIPATLVISLLFFYLTGLTINVISLAG
                     LILGIGMMIDNSIVIIENITQWIDRGESLLNACIKGTNEVITPLISSVLTTCAVFIPL
                     IFLSGIAGALFYDQAIAIVIGQGVSLIVGITLLPTLYYLIYKRRWEGLLAVLVKKVNI
                     KGIEKSYEGGMDFFFRKRKMILVSFFLFIALMIWLFIAMKKERFPAVRQDEFVVTIDW
                     NENIDPRENLERCLRIINSSSDLIKQTNSFIGEQQFLFNRDYDLSYTQSKIYIKATGY
                     DAIQEIKNNISNFIVSNYPDAVVETAPQQNIFEKIFSASKVPLVAEVTLLKEKTIPSP
                     EKLVESVNRLKLLLHDKEISVPPFEDKIMLRLDPIRLILYNIDPDIVYTTLRTALNRS
                     HIGELRSSGEILPVVLAGRERLINDIISSEFVPTRNASLVPLKELVEIQRIHDYKFIT
                     GGMHGEYIPINIFANTRSPGLLMSEIRSVITSDSSLDVQFSGGLLEGQRLFREMAVIL
                     VISVLLLYFILAAQFESLSQPFIVMLELPIDIAGALLLVKLAGGTINIITMIGLIVMS
                     GVIINDSILKVDTINNLRREGMGLLEAIYTGGARRLKPIIMVALAALVSTFPMLVAGG
                     LGSELQRPMALALIGGMSLGTVVSLFFIPLAYWYIYRNLERKY"
     gene            complement(9889..10128)
                     /locus_tag="PLG42_03065"
     CDS             complement(9889..10128)
                     /locus_tag="PLG42_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU30225.1"
                     /translation="MLKTNLVDIPLEIPRYHNSSFEPVVVVQKHQRMSSRIEHAIVTM
                     YFRGMTKKDIEETIKDIYGINVSESSIFNITNTIK"
     gene            10491..>11892
                     /locus_tag="PLG42_03070"
     CDS             10491..>11892
                     /locus_tag="PLG42_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30226.1"
                     /translation="MSGKHKIISFKILLAFNCTIPVLLSSCSQERPAIDRFTLVNRHN
                     IEITTIDSLNSLSVGNGRFAFTVDITGLQTFHEYHSKGIPLGTMADWGWHREINTRGY
                     SLQQVYRTWDVHGRKIDYVHRYSDPRDSLRYRASEWLRENPHKIHLGMIGLDFPSSDK
                     PFAGLIENPHQKLDLWRGEIESRFMAEGKMVRVKTVCHPELDMVSAEITSSLLSERKL
                     RIRIHFPLGVPGQTAYDFTRPNKHITEILSGTDTTVLFRRRQDDDVYYVKLYKPHSAL
                     VRGGERHLFYLEAHPGDTVLEFSCLFSKTMPVDSALPGFSVTEAVSASFWRDFWSTGG
                     AVDFSECTDPRAAELERRVILSRYLTRIQCAGGYPPAETGLSYNTWYGKFHLEMHWWH
                     GVHFALWQKEEILENQMKYYEKIYDKALATARHQGYDGARWPKMTDPQGNESPSNVGP
                     YLIWQQPHFIYFAELLY"
BASE COUNT         3756 a         2242 c         2321 g         3573 t
ORIGIN      
        1 ctccaattaa ttgaatttga ctccaatagt attggaaatt tatttatatg gttttaaatt
       61 cgatagtctt tcttctaaga aaacatattg taattattta gtgtcaaaaa aattaatatt
      121 taaaaaaatg aaaaagattt ttaaaatttt atcagtttta acagtgacca ttgcttttac
      181 aataagcatt ttctttttaa gcaatagaga aactaaagct gattacccgc cacaacgcta
      241 ttgttggttc tggtcagact acccacaata ttgttggtgc gatttaaaga taggttatgt
      301 ttgttatgga tctttatgtg ttgaacttac tgtaagatgt gaagatatta ctccatagtt
      361 aaatataatt ttattttttt cttttggctt aataggtgtt aaaaataaag ttattacttt
      421 tttttaaatc atgtataatg aaattaatac atatttattt cggatttttt ctggtggcat
      481 tttattgttg tactgaaaaa gagggtaaga attttaagga tatatataca ttagcttcaa
      541 ctaatcagat aattgaaatt aagttagatg acaatcaagt taatctttca ccatttattc
      601 aggttttcaa tcataacgga ggtaattttc ttgctttttt agactttgat ttgatatcta
      661 ttaatattta taatattgac tctcagataa aagttagatc tatagatttg aaatctgaag
      721 aattaaaacc tttccagagt atagttgcat tctatataat taatccggat acaattttat
      781 tagtgaatat cagacctcct gtaattgttt ttattgatag tgaggataaa atagtaaaaa
      841 aaatacctgc tggaaatgaa ggagaattta ctgctactat accatgggca gggcaaaagc
      901 ctttattatc aggtaataaa ttgtttttag gccaaatgtt tagctttatg gaaacaaagg
      961 gaaaattgac aggggaaaat atcggttcta caaaaattgc aatcagcatt gatcttttat
     1021 ctggcttaaa aactttatat tctctgactt accctccgtc ccttattgga agcgatgtct
     1081 cgaacatgaa agtatgcagg actcttggtt ataataatag ttttatttat tgtttcagcc
     1141 tttttgattc gctctacata acgaatgaag aacatgtgtc ttttaaaagt aaaaaaatag
     1201 aaaccaatca taaattttct ttttcaaggg aacactggaa atatataaat gatatacaaa
     1261 aaggaatgag agatgagcta ttgcatgatg atattaaaaa tatattttat gataaataca
     1321 gagaatgtta ttatcttttg atacgtgaag gacaaacaga tattgaccag tatgcacctg
     1381 actttgcaac aaggcttcaa tatggtaaat gctttataat tattctcgat aaaaacctga
     1441 aacaccttgg agaagtatat ttccccgata atgtttatgc gttccagatg atgtttataa
     1501 ctcctgaagg attatatatt tccagaaatc atgtaaggaa ccctgaatat gacgaaaagt
     1561 atttaagatt tgaatgtttt acgttaagaa aaataaatga gtaaaagaat attgttactt
     1621 ataatcgttt ttttttatta agttgtaaaa attatcgaga gtcaaataaa ctgatttctt
     1681 ttttatcaga atataatgtt acattgaaca catgtaaagt aattatcatt attcctgctg
     1741 aagcatgttc atcttgtatc aaactttcat ttgaatatat aaaaggaatt aattctgact
     1801 atcttttggt tttatcatct ttttatggga aatcaattaa ttatcttatt gaaaattact
     1861 gccttgataa aactaaatta ttaattgaca agaagaatat ggcaagtgct ctctggctta
     1921 ttgaaccaac tggcccaaca atttatttag taagaaataa gagaatttat aaaaaatatg
     1981 aagctgaaaa aattttaaaa aatccggaaa tatttattga aattaatact ttccttacag
     2041 attgtaaata aaaatgtatg ctgtcaaaat ataaattcaa aatacttcaa gttaaaaaca
     2101 ttattctttt taatttaagt tgctggttag agaatttaaa aatgcactgc aatttacgta
     2161 tttattgacg atcttttgaa agaaatcgga cataaagtac caaccacccg gaatgcggga
     2221 aaaaatcgtt ttaacagaag aatccattga tgaaaaaact ttacagatag caacttgagt
     2281 taattaaaaa atatcataat tttatattta attatctgag aaagtcaaat atgtcaatat
     2341 gaaagtcatc ttttattctg ctttacttac gggcatagtt atgttttatt cctgcaaaca
     2401 aaaaccttcc cagagcttag aagaaatagc aagaagagct gcacgtcccg aagctattat
     2461 agtcaaaacg gtcaaagccg aacgaactac cttctacaat gaactgatca gtaacggcaa
     2521 aatattctca tcaaaaaaag cagtaatacc tttcagagtc agcggtatca ttaaagagct
     2581 ctatatccgg aacggtcagc gtgtaagcga gggagaactc cttgcagtta tcgaggactt
     2641 tgactaccgc gtccagctgc aacgtgcacg acaaaatctc gaaaaagccg aaataaattt
     2701 cagggacgat atacttacca atttctcggc aaatgataca accggccttc ctgcatcaaa
     2761 aattaaaata tcacgtattc ggtcaggtct agatgatgcc ctgaccgcac tcgcacaggc
     2821 tgaatataac tataacagca cccgtatcac ggctcctgtt tcaggagttg tatcaggcat
     2881 ggaagcacgc caatggaacc caagccagaa ctttaaaaac ctttgcacta tagcagatga
     2941 caggataatg gatgtggaat tccctgttac tgaatcggaa tatctgatgc tcagagtagg
     3001 tatgcccgta agcataattc cttttgttaa cgacagttta aaaatcaatg gaaccataac
     3061 ccagataaac cctcaggttg acgaaaacgg aatggtaaaa gtgacagctt cttttgccaa
     3121 caaagccaga ctgatagatg gaatgaatgt cagagtactt ataagaagac ctgtccccga
     3181 caggattgta gtgcctaagg aagcccttgt ccgccgccaa ggcagagatg tgatatttgt
     3241 gcggcaggac tcatttgcaa tatggaaata tgtgacagta gaagctgaaa acagtacaag
     3301 tttgtctatc agtgaaggta ttgaacccgg cgatctggta ataataagcg gaaacataaa
     3361 cctggcacac gaaactgtcg tgagagaaca atgagtgctt ttagaataaa tatctttttc
     3421 gttgtactta taataacagg tattgctatt actccgcttc tctccctgca gttcaatcct
     3481 acccgatacc ttccttcttt gactatcagc ttctcatggc caaacgtacc tgtgaggatg
     3541 gtggaacagg aagtcacaac tgcaatcgaa ggagtcctct caaccgtttc gggaattacc
     3601 agaatcaggt ctgtcacccg tgaaggtatt ggttctatta ctgtcgaatt cgaccgaaga
     3661 actgacctcc aggctaagag gttcgaagtg gcttctctga tgaaagaagc cttcaaacgg
     3721 ctccccgaag gtgtttcata cccctctgta agaatgaatc tgcctgccat cggatcgtcc
     3781 ggttcactga tcttaagcta tcagatcatc ggaaacacaa gccagtcgta tattgctgat
     3841 gtagctgagg aactcataaa accggccatc gcaactatcg atggagttta cagcgttgac
     3901 gtgtatggtt caactccgcg cgaatgggaa ataacctacg acctgaataa actttccgtt
     3961 ctcggcctac aaccaactac cctcactgca gctataaaga attatatgct tgagagagaa
     4021 ctgggaggag gacatgagct tacaacagaa agatctgtaa aaaagacata cataacactt
     4081 gccggcaaca gaaccgatac aatcaaatgg tatgatatcc caataacaag catttctggc
     4141 agaatagtca cacttggaga tgttgctact gtaagactca aagatcgcca gcctgtaagc
     4201 tattatagaa taaatggact taatacaatc aacctcaatg taactgctgc aaaaaatgtc
     4261 aacaatataa aagttgcagg cgaagtaaaa aaaactatcg aaaaaatcaa aaaggaactt
     4321 cctccgggtt acagcatccg taactcgctg gataatacag tattcatacg tcaagaaata
     4381 tcaaaaaacc tgttccgtac tgtactttct gttgtgcttc ttttgctttt tatatttgcg
     4441 ataaccagag aattccatta tcttctgatt atagctatca gcctcttttc aaacatactc
     4501 atagcattca ttttttacta cctgttccat ctcgaaatac atctttattc ccttgcaggc
     4561 ataactgtat catttggaat aataatcaat aataccatcg tgatagtaga tcacctgagg
     4621 caccgtagaa atatgaaagt gggtatatcc cttcttgctg caacactgac tactatcggt
     4681 gcactttcag taatcttttt cctcgatgaa gatatgaaac ttaccctttc cgactttgcg
     4741 gcagtggtaa ttattaatct atccgtatca cttgtaatag ctctcttttt tattcctgcc
     4801 ctgatggaga aaataagact taaatcccat cacacttcat atgccaactg taaaaaaaga
     4861 gcagcagtga aatttacaca tcaatataag aatataattg actttataat acagcgccgc
     4921 acagctttaa ttattatggc tattcttgtt ttcggactcc ctgtcattta tctgcccgac
     4981 agattacctc ccgaacagag tacaattatg caggaagagc aactaacttg gctccagaaa
     5041 ttatataaca agagtctcgg aaatccgaaa tatgtacaga aatttaaacc cgcgataaac
     5101 aaagtacttg gtggtacgct gaggctattc agcgaaaaag tcaagaacag taggttctac
     5161 tattatggta gctcaggtga agttcagagg actcagataa ctgttgacat aggcttgagc
     5221 gaacctggtc tgactgttga aaatataaac aatatatgca tgcggctgga gaatatgctt
     5281 gctgaatacg atgaaattga cctgtttacc acaactgttt acagtgctga agatgcaacc
     5341 gtaactgtaa cttttaaacc agaatatgac tttacagttt ttccgtacat cctgaagttc
     5401 aagattgagg attttatgaa ttcaatcgga agctatcata caatagttta tggggtgggg
     5461 aaggccttca gtaaccaggt ttacagcgat tatatcaggg gcacatacac cataaatatg
     5521 cgaggataca actacgacga actttacaac tattgtgagg gactgaaaga gagactaatt
     5581 aaaagtgcaa gaggaagaat aaaggaagtc ttttttttag cttcctctga caggtttaac
     5641 gtcaggaaga attaccgaaa cacgcttctg atggacaggt cttatctggc ggcttatgac
     5701 gccgatattg tttctaccta tgcaggagcg taccgtttat cgggaaattc tttaactgca
     5761 ggcaatgttt acactgaagg ctttattgca cctatatcat tgaaaacact ccaggcagag
     5821 gaatttgatt actggaactt cattaacgag ccaattctta taagaaatgg tatgcagatg
     5881 aaactgaaag attttgccgg tgttgtctct gaagtttcag ataataccat aagcagggag
     5941 gatcaggaat atgtaattta cgttacatat gattttattg gcaactatga actcggcagg
     6001 atgatccttg aacgtaacgt aaaggaaaca aattcattac tgcccctcgg atatacagca
     6061 caaatttcaa cctattcatt ttcatggggc agtagcaagg caaactacct gctcatcttt
     6121 ctggttgtcc ttattatatg gattgtctgc tcaattctgt tcgagtcgct gaaacaacca
     6181 cttatagtca ttagtcttat ccctttctcg cttatcggag tgtttcttac gtttttcatt
     6241 tttaacatta atcccgatga aggcgttttt gctgcgatga tacttctctg cggaatcgtt
     6301 gtaaactcat caatttacat cctgggcgat tacaacagtc tgaggcggga aaaacccgga
     6361 atggatgaaa gaagacttta ccttaaagct ttcaacggta agataatacc tgttattctg
     6421 acaatttttg caactattat gggtcttatt ccgtttctgc ttactggtag agatgaacgg
     6481 ttctggttca accttgctgc cggtaccatt ggtggactga tattcagtat agttggattg
     6541 cttttctatc agccattgat gttgaagaaa aagggtagat aaactaagga attagtaaga
     6601 ctattgcgac tttcggagtt taaaaacttt tcaaccataa gaaaatgcaa agtgatggga
     6661 aatgtagtat ggaaaataag gaaattatat aaatgccgga aaattaaatt catattcaga
     6721 taattatggt aaggtttcta ataacccgcc cggttgcggt actaatgacc ttcatatcgg
     6781 ttataatgct cggtatcatt tcttccagca ggcttccggt ttcgctgatg ccagatgttg
     6841 acattcccga aatcacagtg gctgtgtccc ggcccgatgt cagcgcaagg gagatggaga
     6901 acaccgtagt gagtatcctc agaagaaacc ttctgcaggt tcccggtctg gccgacctga
     6961 gatctgaatc gcacaatgga agtgccctga tacggttatc gttcaaatac gggataaaca
     7021 tcaatcttgc ttttatggag gtcaacgaga aaattgatgg ggccatgaac aatcttccgc
     7081 gcgacatcca acgtccaagt gtaataaaag ccagcgcaag cgatatacct gtatttatgg
     7141 ttaatatcac actttcggag ggttatgaaa gcagtgaaaa attcaccgaa ctaagcaact
     7201 ttgccgaaac gataataaag aagagaatcg aacagctacc cgaagtagcg atggctgata
     7261 taaccggaca ggtaaagaga gagctctata ttcttcccga cagaaaaaaa atgatgagtc
     7321 tcggaataac tgagcaggat ataataaagg cggtagagct caataacctt aatattggca
     7381 acattatggt gagggatggc aaataccttc tgaaccttga attcaattct tatctcaaag
     7441 accctgaaga cgttaagaat atatatttga atgcagggag tgccaggatt cttcagctta
     7501 aagatatagc tgatgttgga ataagagagg aaaaaccccg tgggctatac tattcaaacg
     7561 gcaaaagagc tgtagtattg gctataatta aagaagcctc cgcgcagatg gaaacactga
     7621 agtcaaagat gggagagctg ctcacccagt tcaggaaaga ttaccctcag atggattttg
     7681 aaatctcaca ggatcagact cttctgctcg attattcaat ttcaaacctc agacaaagcc
     7741 tctgggcagg tggattactg gcattctttg tgatgttctt cttcctgaaa gatgcaaaat
     7801 cgcctctaat aattggtttc agtattcccg caactcttgt aataagtttg ttgtttttct
     7861 atcttacagg tcttactata aatgttattt cacttgcagg ccttatactt ggtattggca
     7921 tgatgatcga caactctata gtaataattg aaaacataac gcaatggatt gataggggag
     7981 aaagccttct taatgcctgc atcaaaggaa cgaatgaggt gataacacct cttatttctt
     8041 ctgtacttac aacatgtgca gtgtttatcc ccctgatttt tcttagcggt atagcaggtg
     8101 ctctttttta tgaccaggca attgcaattg taataggaca gggagtctca ctgattgtgg
     8161 gtataacact tctacccaca ctttattatc ttatttacaa aagacgctgg gaaggtttgc
     8221 ttgcagtact ggtaaagaaa gttaatataa agggtataga aaaaagttat gagggaggga
     8281 tggacttctt ctttcggaag cgaaaaatga ttctggtatc ctttttctta tttattgcat
     8341 taatgatatg gcttttcatt gcaatgaaga aggagagatt tccggctgtg agacaggatg
     8401 agtttgtggt gactatcgac tggaatgaga atatagatcc cagggaaaat cttgaaagat
     8461 gtcttcgcat aattaattcc tcgtcagact tgataaagca aactaattca ttcataggtg
     8521 aacagcagtt cctgttcaat cgcgattatg acctttcata tactcagtcg aagatttata
     8581 ttaaagccac tggatatgat gccattcagg agataaaaaa taatatctct aattttattg
     8641 tgtcaaatta ccccgatgcc gttgttgaaa cagcccccca gcagaatatt ttcgaaaaga
     8701 ttttctcagc atcaaaagtg cctctggttg ctgaagtgac gcttttgaag gaaaaaacaa
     8761 tcccatcacc agaaaaactt gttgaatcag taaacagact taagttgtta ttgcacgata
     8821 aagaaatatc tgttcctcct tttgaggata aaataatgct caggcttgat cctataagac
     8881 ttattctcta caatatcgac cctgatattg tatacacaac ccttcgaacc gctctgaacc
     8941 gtagtcacat tggagaatta aggtcctcag gagaaatttt gcctgttgtt ctggcaggaa
     9001 gagaaaggct gataaatgat ataatatcat cagagtttgt accaaccagg aatgcatctc
     9061 tggtcccctt aaaagaactt gtggaaatac agaggataca tgattacaaa tttattactg
     9121 gaggaatgca tggagagtat attccgataa acatttttgc caacactagg tcaccgggtc
     9181 ttcttatgtc agaaatacgt tccgtgatca cttcagattc ttcgctcgat gtacagttta
     9241 gtggcggact acttgaaggg cagaggctat tcagggagat ggctgtgatt cttgtcattt
     9301 cagttttgtt gctttatttc atccttgctg ctcagtttga atcactttcg cagcctttca
     9361 ttgtaatgct cgagctgcct atcgatatag caggtgcact gttgctggtt aaacttgccg
     9421 gcggcacaat caatatcatt acaatgatag gtctcattgt aatgtcgggt gtgatcatca
     9481 atgactccat actgaaggtt gacacaataa acaaccttag aagggaaggg atggggttgc
     9541 ttgaagcaat atatacaggt ggggcaaggc gactgaagcc tataataatg gtggctcttg
     9601 ctgctttggt atccacattc cccatgcttg tggccggggg ccttggttcg gagttacagc
     9661 gtccaatggc acttgccctt ataggcggta tgtcactagg aacagttgta agcctattct
     9721 ttataccgct tgcttactgg tatatttaca gaaatttaga gagaaaatac taacagaacg
     9781 agcctgttta aatttttgtg taaactggca gcagccggga ttaaaacata ccgaccatac
     9841 aaccacataa accggatcaa gtggactctg ttgccactct tttatatcct attttattgt
     9901 attggtaata ttgaatatcg aactttcact gacattaata ccataaatgt cctttatggt
     9961 ttcttcaatg tcttttttag tcatgcctct gaaatacatc gtaacaatgg catgctctat
    10021 tctggaagac attctttgat gtttttgtac tacaacaaca ggttcaaatg aagagttgtg
    10081 ataacgaggt atttcaagcg gaatatccac cagattggtc ttgagcattt tttactgtaa
    10141 ctattacgac tgttacctgt gttacaacca gcagaataat ttgaaaattg aatttttctc
    10201 tctatccaac cggactttga gttcgttttt ccggctccag ccaattcggt gttccaaatg
    10261 cagctgccaa tccataaata caaaattatg aacactacaa acaatcataa tatttgaaaa
    10321 tttttagtca ttatatttaa caatcaatat aaaatacggg ttgtgccatt taaaaattgg
    10381 aaagtaaaca ttttgcgtac atacaacttc tcgctccatc attcgacatt tcttgttcat
    10441 aattcattat attcttttca gaaagtatat ttgtataaat ctatcctgca atgtccggaa
    10501 aacataaaat tatttccttc aaaatattat tggcctttaa ttgcacaatt ccggtacttc
    10561 tttcttcgtg ttctcaggaa agaccagcta tcgaccggtt tacccttgtt aatcgccata
    10621 atatcgaaat cacgaccatt gacagcctca actccctctc tgtcggtaat ggcaggtttg
    10681 cttttaccgt tgatatcaca gggcttcaga ctttccatga gtatcactca aagggcattc
    10741 cgctaggtac aatggccgac tggggctggc atagagaaat aaatacccgg ggctactcat
    10801 tgcaacaggt ttatcgcaca tgggatgtcc atggccgcaa aattgactac gtacaccgat
    10861 actccgaccc gcgcgactct ctgagataca gggcttctga atggctcagg gaaaatccgc
    10921 ataaaataca tctcggaatg ataggccttg actttccttc ttcggataaa ccatttgcag
    10981 gccttattga gaacccacac cagaaactgg atctctggag aggtgaaatt gaaagccggt
    11041 tcatggctga aggaaagatg gtaagggtaa aaacagtctg ccaccctgaa cttgatatgg
    11101 tctcggcaga aattacctca tcccttctct ctgaaagaaa actcagaata aggattcatt
    11161 ttccgctcgg agtcccgggt cagacggcgt acgacttcac caggcccaat aaacacataa
    11221 cagagattct atccgggaca gatacaacag ttcttttcag acgcaggcag gatgatgatg
    11281 tttattatgt gaagctttac aaacctcact cagcgcttgt taggggaggg gagagacatc
    11341 tgttttatct tgaagcacat ccgggtgata cggtgcttga attttcgtgt cttttttcaa
    11401 agaccatgcc tgttgactct gccctgccag gtttcagtgt aacagaggcg gtttcggctt
    11461 ctttctggag agatttctgg agtaccggcg gagctgtcga tttttcggaa tgtaccgacc
    11521 cgcgcgcagc tgaactggag agaagagtca ttctttcccg gtatcttacc cgcattcagt
    11581 gtgcgggtgg ttacccgcca gccgaaaccg gactctcata taacacatgg tacggcaagt
    11641 tccacctcga gatgcactgg tggcacggtg tccattttgc cctgtggcag aaagaagaga
    11701 tacttgaaaa ccagatgaag tattatgaaa agatatacga taaggctctt gccactgcgc
    11761 gccaccaggg ctacgacggg gcgaggtggc ccaaaatgac cgaccctcag ggaaatgaaa
    11821 gcccgagtaa cgtgggccca tatctgatct ggcagcaacc ccatttcatt tatttcgctg
    11881 aactgcttta cg
//
LOCUS       DAOSNB010000040        11852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5356_length_11852_cov_5.085530, whole genome shotgun sequence.
ACCESSION   DAOSNB010000040 DAOSNB010000000
VERSION     DAOSNB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_11852_cov_5.085530"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1586
                     /locus_tag="PLG42_03075"
     CDS             <1..1586
                     /locus_tag="PLG42_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU30227.1"
                     /translation="TFVTVKRNHILLLALFFLITISGLTIIQLYWIRNAISVTDQQFR
                     YQANKVLENVVLTLEEKEILNRIISGIDSSEQSSGKTILSSPYFSEKSGGEDLKQLEI
                     YGIGSSEKPVIINRNGQKIIISSNDESLFGAEDTLEILPENTGAGFRRRVVNKIISLE
                     TILGKIFRTNLDVKERINPDDIIKTIDESLRNAGIPLKYEVAVTSGRYGIVWKTPGYR
                     DASGTNRFIRQLFPNDPVPGQNQIIMYFPQEKQYKFEKIKLLGVLSLFFTSLLILLSA
                     GTLILIFRQKKLSEIRNDFIHNMTHELKTPISTISLAAQMLADKSIADHAKNIDDLAR
                     IVADESLRLKYHVEKILQTAIFERVKLKLHLADTDIHVLLNKVIESFALQVKSVNGKI
                     IRDFRAKDPIVLVDEAHFINAISNIIDNAIKYSRDIIEITISTRNLKKWLVISVEDKG
                     IGIERENLKRIFEKFYRVPTGNIHNVKGFGLGLSYVKKVVEDHNGTIKAESQPGKGSR
                     FTIYLPKNKKNENKENTTS"
     gene            1558..2244
                     /locus_tag="PLG42_03080"
     CDS             1558..2244
                     /locus_tag="PLG42_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU30228.1"
                     /translation="MKTRKILLAEDDKNLGLLLRNYLNAKNFETKLCEDGKEALEIFL
                     KENFDLCILDIMMPEIDGITLAVEMRKVNPDIPVIFLTAKNLKEDIIEGFKSGADDYI
                     TKPFSMDELLYRIEAILRRASGKKEHKKEDIYKIGEYTFDAVKQLLSFRDKVIKLTTK
                     ESELLELLCIHKNEVLERNYALKAIWTDDNYFNARSMDVYITKLRKYLKQDPSVKILN
                     VHGKGYKLLA"
     gene            complement(2425..4212)
                     /gene="lepA"
                     /locus_tag="PLG42_03085"
     CDS             complement(2425..4212)
                     /gene="lepA"
                     /locus_tag="PLG42_03085"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOU30229.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLEKTKTLSDRELVEQVLDNM
                     ELEKERGITIKSHAIQMEYYFEGENYILNLIDTPGHVDFSYEVSRAIAACEGAILVVD
                     ATQGIQAQTISNLYLALDHNLEIIPVLNKMDMPTAMPEEVKDQIVDLLGCPREDIIEA
                     SAKTGMGVDRILHDIVKKIPPPSGDPDAPLQALIFDSMYNPFRGIIAYYRIVNGSIRK
                     GDHVKFFNTGREYEADEIGVLKLKLVPKEFMGTGDVGYIISGIKESSEVRVGDTITHV
                     DRPCENAISGFEEVKPMVFAGIYPVDADDFEDLKSSIEKLRLNDAALSYVPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVSYRVYTKKGEMIEVHNPSGLPPV
                     TQIERIEEPYIMAQIITLADYVGPIMNLCIDKRGTMRNQVYLTRDRVELTCEMPLSEI
                     VFDFYDKLKSISRGYASFDYYYTSYKEADLVRLDILLNGEMVDALSTLIHRSHAYEFG
                     RKMCQKLKELIPRQQFEIAIQAAIGSKIIARETVKALRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNVQVPQQAFLAVLKLD"
     gene            complement(4347..5981)
                     /gene="groL"
                     /locus_tag="PLG42_03090"
     CDS             complement(4347..5981)
                     /gene="groL"
                     /locus_tag="PLG42_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654643.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOU30230.1"
                     /translation="MAKEIKYNNDARALLKEGVDYLANTVKVTLGPKGRNVLIEKKYG
                     APLVSKDGVTVAKEIELSDPYKNMGAQLVKEVASKTGEDAGDGTTTATVLAQAIINVG
                     LKNVTAGANPMDLKRGIDKAVAKVVESLKSQARVIGDDLSKIEQVARISANDDEEIGK
                     LIAEAMSKVHKEGVITVEEAKGTATTVEVVEGMQFDRGYISAYFVTNAEKMEAELENP
                     YILIHDKKISTMKDLLPILEAVAQTGTPLLIIAEDVEGEALATLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTGGTVISEEKGLKLEHATLDMLGRAEKVTVDKDDTTIVN
                     GAGDKSQIKARINQIKQQIANTTSDYDKEKLQERLAKLSGGVAVIYIGAASEVEMKEK
                     KDRVDDSLHATRAAIEEGIVPGGGVAYIRALKALENLKGDNEDQNTGIEIVKRAIEEP
                     LRQIAENAGREGSIVVQEVKKGKNDYGYNAQADRFENLYEAGVIDPVKVARIALENAA
                     SVAGMFLTTEAAVVEEKEEKQGAGKGMNPGMGDMGY"
     gene            complement(6012..6281)
                     /locus_tag="PLG42_03095"
     CDS             complement(6012..6281)
                     /locus_tag="PLG42_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOU30231.1"
                     /translation="MKGKVLAGKVLVKPHEAEAKTASGIIIPDSAKEKPRQGTVVLVG
                     APKKDEEMEVKVGDVVLYGKYAGQELTIDGEEYLLLSQSDILFIQ"
     gene            complement(6536..6886)
                     /gene="secG"
                     /locus_tag="PLG42_03100"
     CDS             complement(6536..6886)
                     /gene="secG"
                     /locus_tag="PLG42_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOU30232.1"
                     /translation="MYVFISVLIIIACILQIMIVLVQNSKGGGLAANFTSAGQSMGIR
                     KTADFLEKSTWTIAISIFVLSLLATAAIPRGGETQRSRLEQQIQNAIDPNAIPTLPSA
                     TPPASSENPQQNKQ"
     gene            complement(6901..7917)
                     /locus_tag="PLG42_03105"
     CDS             complement(6901..7917)
                     /locus_tag="PLG42_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30233.1"
                     /translation="MNRSDFLNLLKDPGKINQGVTAELRELVGIYPWFQSAHLLLLKG
                     MHTSGDVRFENQLRQSALFVASREVLYYLLHPVNTRITGMEKPVVKITIPDVPDSQQV
                     VLDTARNSEEVIRMLEREEEEVALASGRKAPGRVKTTILATDSNIDESATVTIIYEDG
                     DETIKESLVYIDPSITQGKSLDILELDESKPVSGTPEEIPEEIPAEIPPELLQEVPAE
                     IPSEIPAEITLELTSEVDTSNNDVTEVSKPLSQAELIERFILSNPKIEPVREKRDEPL
                     VDISRPFTEETGGFVTETLAKIYVSQGYFSKAIDIYEKLSLKYPEKSSYFASRIEEIK
                     KLIK"
     gene            complement(7924..8460)
                     /locus_tag="PLG42_03110"
     CDS             complement(7924..8460)
                     /locus_tag="PLG42_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOU30234.1"
                     /translation="MVLTCKYFLIKRIYASVLIVLLFFSASSCRVSYSFSGASISPQV
                     KTLSVQYFQNRASLVQPGLSQALTDALIDKCRAQTRLSLVNGFGDVNFEGEITDYNTR
                     PLTVGADSRAAMNRFTITVKVKFTNSVEPQYNFEKTFSRYEDYDSRLNLSDVEKDLSE
                     KIIALLVEDIFNAAFVNW"
     gene            complement(8444..9682)
                     /locus_tag="PLG42_03115"
     CDS             complement(8444..9682)
                     /locus_tag="PLG42_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOU30235.1"
                     /translation="MRDLQSIKQRFGIIGNHPGLNRALDIAVQVAPTDLSVLITGESG
                     TGKEIFPQVIHSYSTRKHGQYIPVNCGAIPEGTIDSELFGHEKGAFTGATDSRKGYFE
                     VADGGTIFLDEVSELPLSTQVRLLRVLETGEFIRVGSSKVQKTNVRVIAATNVDLPVL
                     IAEGKFREDLFYRLNTVPIKIPALRERGEDIILLFRKFAADFAERYRMPPIRLTPGAQ
                     AMLLSYDWPGNVRQLKNITEQISIIENEREITEEILRRYLPDYSAVKLPVVMRQPDEK
                     SFSSEREILYKILFDMRNDVNDLKKLVLGMIEKEGDMNFKQEHTGVIRNLYGEAKIES
                     TPATSVQGTAVDFKSRHSADIQDTEEIVEESLSLSEKEAELIVKALEKHNRKRKQAAK
                     ELGISERTLYRKIKEYGINM"
     gene            9757..10539
                     /gene="kdsB"
                     /locus_tag="PLG42_03120"
     CDS             9757..10539
                     /gene="kdsB"
                     /locus_tag="PLG42_03120"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOU30236.1"
                     /translation="MKVQEKLHITGIIPARYGSTRFPGKPLATIGNKPIIRLVYEQAL
                     KAIENVYVATDDERILKAVVEFGGKAVMTSTLHRSGTDRCAEAVKIIEETTGRSTDVI
                     INIQGDEPFVKPEHISLLASCFDDPSVEIATLIRRVKQGENIFDENHPKVVINIFGDA
                     IYFSRSVIPFIRDAGKPEWNLKHNYFKHIGIYAFRKETLFKVTSLEPSSLEIAESLEQ
                     NRWIENGLKIRTAVVDWESISVDTPADLEVARRFYEKMRLED"
     gene            10596..10964
                     /locus_tag="PLG42_03125"
     CDS             10596..10964
                     /locus_tag="PLG42_03125"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30237.1"
                     /translation="MKSLFLILAAFVISSSLFAQKDSTALALKSSNKLSASDNSTSVS
                     IYPVPVKDGQFTIKSIKEISSVRITNIIGQEIFKANYNNPEKEIQVSLDNPNRGMYIV
                     VITFTDRTRLVKKITTEGTQ"
     gene            complement(11007..>11852)
                     /locus_tag="PLG42_03130"
     CDS             complement(11007..>11852)
                     /locus_tag="PLG42_03130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOU30238.1"
                     /translation="PYGTIYNPVSIANTLDMIMSGRVFTKNDLFYYDNLYHSFYHYTN
                     FSSVDPDHAVEKINDSNAKAHDFLKQAQFLFVTFGTARVYKLKEGGMVVSNCHKLPST
                     MFSNELLEVEGICELWEKLLDRIAAFNPELSVIFTVSPVRYLKEGAHGNQVSKSILFL
                     AIEKLLGHKISPGYFPAYELLMDDLRDYRFYAADMIHPSETAIDYIFNSFMDAFFEKE
                     TFSIFNEAMEIVKARNHRFISTNPAAVKDFAARTLRKISQFEKRVPEADFSEEKKYFN
                     LLIEG"
BASE COUNT         3482 a         2575 c         2221 g         3574 t
ORIGIN      
        1 ttacttttgt aacggtgaaa cgaaatcata tactgttgct agctcttttc ttcctgatta
       61 cgatatcagg attgaccatt atccagcttt actggatccg taatgctatt tctgttaccg
      121 atcagcagtt tcgctatcag gcgaataaag ttcttgaaaa tgttgtactt acacttgaag
      181 aaaaagagat actaaaccgt attatttccg gaatagattc ttcagaacag agctctggaa
      241 aaacaatatt gtcctctccg tatttttctg aaaagtcagg tggtgaagat ttaaaacagc
      301 ttgaaatata tggcataggt agttctgaaa aaccggtaat aataaaccga aacggtcaaa
      361 aaataattat atcatcaaat gatgaaagtc tcttcggcgc agaagacaca cttgaaatat
      421 tgcctgaaaa taccggagca ggtttcagaa gaagagtagt aaacaaaatt atatcgctcg
      481 agactatcct gggcaaaatt ttcagaacca accttgatgt aaaagaaaga ataaacccgg
      541 acgatataat taaaacaatt gatgaatcac ttagaaatgc tggtattcca cttaaatacg
      601 aagtggctgt tacatcgggc aggtatggta tcgtttggaa gactccaggt taccgggatg
      661 catctggcac caacaggttt ataaggcagc ttttccccaa tgatcctgtt cccggacaaa
      721 atcagataat tatgtatttc ccgcaggaga aacaatataa attcgaaaaa ataaaacttc
      781 tgggtgtcct ttcactgttt tttacttcac tgctgatatt actttctgca ggaaccctga
      841 ttcttatttt caggcaaaaa aaactttccg aaatcagaaa tgacttcatt cacaatatga
      901 ctcacgaact taaaacaccc atctcaacaa tatcccttgc tgcacaaatg cttgctgata
      961 aatcgatcgc cgaccatgca aaaaatattg acgacctggc cagaatagtt gcagatgaaa
     1021 gtcttcgttt gaaataccat gttgaaaaaa tactccagac agctatcttt gaacgtgtaa
     1081 aactgaaact acatcttgca gatacagata tacatgtatt actcaataag gttatcgaaa
     1141 gttttgcact tcaggtaaaa tcagtaaatg gtaaaataat aagagatttc agggcaaaag
     1201 atccaattgt ccttgtcgac gaagcacact ttataaatgc tatttcgaac ataatcgaca
     1261 atgccattaa atattcaaga gacataattg aaataacaat atccacaaga aaccttaaaa
     1321 aatggcttgt catctcggtt gaagataagg gaattggtat agagcgggaa aatcttaaaa
     1381 ggatttttga aaaattctac cgggtcccca caggaaacat tcataatgta aaagggtttg
     1441 gactcggact cagttatgtt aaaaaagttg ttgaagacca caacggcaca ataaaagccg
     1501 aaagtcaacc cggtaaagga tccagattca caatatattt accaaaaaac aagaaaaatg
     1561 aaaacaagga aaatactact agctgaagac gataaaaatc ttgggcttct acttagaaat
     1621 tacctgaacg caaaaaactt tgagacaaag ctttgcgaag atggcaaaga ggctctggaa
     1681 atatttttga aagaaaattt cgacctgtgc attctggaca taatgatgcc tgaaatagac
     1741 ggaataaccc tcgctgttga aatgaggaag gttaaccccg acataccggt tatattcctt
     1801 acagctaaaa accttaaaga agatattatc gaaggtttta aatcgggtgc cgatgattat
     1861 attacaaaac ctttttcaat ggatgagctt ctttaccgta ttgaagcaat cctcaggaga
     1921 gcctccggaa aaaaagaaca taagaaggaa gatatttaca aaatcggaga atacactttt
     1981 gatgcagtaa aacaacttct ttcattccgc gataaggtaa tcaaacttac cacaaaagaa
     2041 tcggaacttc tagaattgct atgtattcat aaaaatgaag ttctggaaag aaactatgcc
     2101 ctgaaagcta tctggacaga tgataattat tttaatgcca gaagcatgga tgtttatatc
     2161 accaaactaa ggaaatatct gaaacaggac ccttccgtaa aaatcctgaa tgtccacggt
     2221 aaagggtata aattgcttgc ataaaattta ccctggctct ttaatatata aggccagggt
     2281 aatattaaca ggtcaggtca gtaagggcta gatatgatat tctattattc ccccgttaaa
     2341 gacaaggcca atttaattaa taattttaat tttcaaaata atttttaaca tcattaatta
     2401 aaatttttat ggataattgt tggatcagtc aagcttgagc actgcgagga aggcttgctg
     2461 gggcacctgg acgttaccaa tctgacgcat gcgtttcttt ccttttttct gtttttcaag
     2521 cagtttcctc tttcttgtaa tatcacctcc gtagcatttt gccgttacgt cctttctcaa
     2581 tgccttaacg gtttcacggg caataatttt tgagccaatg gcggcctgaa ttgctatttc
     2641 gaactgctgc cgcggaataa gttctttcag tttctgacac attttccgac caaactcata
     2701 ggcatggctc ctgtgaataa gtgtagagag ggcatcaacc atttccccat taagcaaaat
     2761 atcaagcctt acaaggtcgg cttctttgta tgatgtataa taataatcaa atgatgcata
     2821 tcctcttgaa atgcttttca gtttatcata aaaatcaaac actatttccg aaagaggcat
     2881 ctcacatgtt agttcaaccc ggtcgcgtgt aagatagacc tgattgcgca ttgtaccccg
     2941 cttatcaatg cacagattca taatgggacc aacgtaatcg gcaagagtaa taatctgtgc
     3001 cattatatag ggttcttcaa ttctttcaat ctgggtaact ggcggtagtc ccgaaggatt
     3061 atgaacctct atcatctctc ccttttttgt ataaaccctg taagagacat ttggcaccgt
     3121 agtgattaca tccatgtcaa attccctgtc gagcctttcc tggactattt ccatatgcag
     3181 aaggccaaga aaaccgcatc tgaagccaaa acccagagca gcagaagatt ctggtacata
     3241 ggaaagagca gcatcattaa gccgcagttt ctcaattgat gatttcaaat cctcgaagtc
     3301 atctgcatca acaggataaa ttccggcaaa taccattggc tttacttctt cgaatccgga
     3361 aattgcattt tcacatgggc gatcaacatg agttattgta tccccaactc tgacttctga
     3421 ggattccttg ataccagaaa tgatgtaccc cacatcgcca gtccccataa attctttcgg
     3481 cacaagtttc agcttaagaa cccctatctc atcagcttca tattcccttc ctgtattgaa
     3541 aaattttaca tgatcccctt tacgtattga accgttgaca attctgtaat acgctattat
     3601 tccccggaaa ggattataca tagaatcgaa tatgagtgcc tgcagcggtg catcaggatc
     3661 acctgatgga gggggtatct tctttactat gtcgtgaagt attctgtcaa cgcccatgcc
     3721 agttttggca cttgcttcaa taatatcttc tctgggacat cccagcaggt cgacaatctg
     3781 atcttttacc tcttcgggca tggctgtagg catgtccatc ttgttgagaa cgggtattat
     3841 ctcaagatta tggtcgagtg ccagatagag atttgaaatt gtctgtgcct gtattccctg
     3901 tgtagcatcc acaaccagta tagcaccctc gcaggcggct attgctctgg aaacttcgta
     3961 tgagaaatca acatgcccgg gtgtatctat aagattaaga atatagtttt ctccttcaaa
     4021 gtaatactcc atctggatgg catggctctt tatggtaata cccctttcct tttcaagttc
     4081 catgttatcc agaacctgtt cgaccagctc tctgtctgaa agggttttgg ttttttctag
     4141 cagccggtca gccagggtac ttttcccgtg gtcgatatgg gctattatgc agaaattacg
     4201 gatgtttttc atcatctggc tgctgaaata tgctgcaaat ataaagaatt aacaggtatt
     4261 agcttataag tctcaaatta aaagaaaaag cccttctgat ggtttggttc agaagggcta
     4321 tggcgaatat cctgtaatgc tttattttaa taacccatat cgcccattcc agggttcata
     4381 ccttttcctg cgccctgttt ctcttctttt tcctctacaa ctgcagcttc tgtagtcagg
     4441 aacatacctg caactgaagc tgcattttca agggctattc tggcaacctt aaccgggtcg
     4501 attacacctg cttcgtacag gttctcgaat ctgtcagcct gggcattgta gccatagtca
     4561 ttctttcctt tcttgacttc ctgaactaca atggaacctt cgcggcctgc attttcagca
     4621 atctgacgaa gaggttcctc tatagccctc ttgacgattt cgatacctgt gttctggtct
     4681 tcgttatcgc ccttaaggtt ttcaagagct ttgagagcgc ggatgtaagc tacaccacca
     4741 ccgggcacta tcccttcttc aatggcagcc ctggtagcat gcagtgagtc gtcaaccctg
     4801 tctttcttct ccttcatctc aacttccgat gcagcaccaa tataaattac tgccacaccg
     4861 cctgacaatt tggccagacg ttcctgcagt ttctctttgt catagtcgga agttgtgttg
     4921 gcaatctgct gcttgatctg gttgattctt gccttaatct ggcttttatc gccggctccg
     4981 ttgacaatgg tagtatcatc tttatcaact gtaacttttt cagccctgcc aagcatatcg
     5041 agagttgcat gctcgagctt caatcctttt tcttctgata tcacagtacc acctgtaagg
     5101 attgcaatgt cttcgagcat ttcctttctc ctgtcaccga aaccgggagc tttaacagcg
     5161 caaactttta atgagccgcg gagacggttc acaactaaag ttgcaagagc ctcaccttcg
     5221 acatcctctg caatgataag aagcggcgtt ccggtctggg caacagcttc aagaatcgga
     5281 agcagatcct tcatcgttga tatcttctta tcatggatga ggatgtacgg attttcaagc
     5341 tcggcttcca tcttttcagc attggtaaca aaatatgctg aaatatagcc gcggtcgaac
     5401 tgcatacctt caacaacctc aacagttgtt gcagtacctt ttgcttcctc aacagttata
     5461 acaccttctt tatgtacctt actcatagcc tcagcaataa gcttacctat ctcctcatca
     5521 tcgtttgccg atattctggc aacctgttca atcttgctga ggtcatcacc aataactctg
     5581 gcctgtgatt taaggctctc aacaaccttt gcaacagctt tgtcaatacc cctcttcagg
     5641 tccattggat tggcacctgc tgtaacattc ttcaggccaa cgttgattat tgcctgtgca
     5701 agaactgttg cagtggttgt accgtcacca gcatcttcac cagtctttga agctacctcc
     5761 ttaaccagtt gagctcccat atttttatag ggatcggaaa gctcaatctc ctttgccacg
     5821 gtcacaccat cctttgaaac aagaggtgca ccgtattttt tctcaatgag tacattacgg
     5881 cctttcggtc caagcgtgac ctttacagtg tttgccagat aatcaacacc ttctttcaga
     5941 agtgcacgcg catcattatt gtatttaatc tcttttgcca tatctttaag ttttagtttg
     6001 gttaataaat tttactgaat gaaaagaatg tcagactgcg agagcaacag gtactcctcg
     6061 ccatcaatgg ttaattcctg accggcatat ttgccgtaaa gtacaacatc acctactttg
     6121 acttccattt cttcgtcttt ctttggagcg cctacaagaa caaccgtacc ctgacggggt
     6181 ttttcctttg ctgaatccgg aatgatgatt ccgctggcag tcttagcttc agcttcatga
     6241 ggtttaacga gaacctttcc tgccaatact tttcctttta tttttttcat gttataactg
     6301 tttttaaggt ttaacaatta ttttgcttcc cgttatctgc attttccatg ccaataataa
     6361 tttaatgaca ttttgtaaga agctgacact gccgtaagat ttatgttgta acacaaaaaa
     6421 atttgcctat tgtaattaat tcattgatta tcaacactta aatataaaaa acaaaaaatg
     6481 tcagttcatg acaaactgac atttatattt tcagtcaaaa ctaaagtttt ctactctatt
     6541 gtttgttttg ctgcggattt tctgatgatg caggaggtgt tgcagaagga agcgttggaa
     6601 tggcatttgg atcaatagca ttctgtatct gctgttccag cctggagcgc tgtgtttctc
     6661 ctcctcttgg aattgcagcc gttgccagaa gtgagagtac aaatattgat atagcaatcg
     6721 tccatgtcga tttttcaaga aaatctgctg tttttcttat tcccatcgac tgtcctgcgg
     6781 aggtaaaatt tgctgccaga ccgccaccct ttgaattctg aaccagcaca atcattatct
     6841 gcagaatgca ggctatgatt atcagaaccg aaataaaaac ataaattccc attttcagtt
     6901 ttattttata agttttttga tttcctcaat ccgggatgca aagtaactac ttttttccgg
     6961 atatttcaaa ctcagtttct cgtaaatatc aatagctttt gaaaaatagc cctgactaac
     7021 atatattttt gcaagggttt ctgttacaaa cccgccggtt tcttcggtaa atggtctgga
     7081 aatatctaca aggggttcat ctctcttttc cctgacaggt tcaatcttcg gatttgatag
     7141 aataaatctt tcaataagtt ccgcctgtga aagtggttta cttacctcag ttacgtcatt
     7201 attagaagta tcaacttcag aggttaactc cagagttatt tctgccggaa tttcagaggg
     7261 tatctcagca ggaacttcct gtagcaattc gggaggaatt tcggcaggta tttcttccgg
     7321 aatctcttcc ggagtacctg aaacaggttt actttcatcc agctcaagta tatccaggct
     7381 ctttccctgt gtaattgaag gatcaatgta aaccagggat tcctttatcg tttcatcccc
     7441 atcttcgtaa attattgtaa ctgttgcaga ttcatctatg ttagagtcgg ttgccagtat
     7501 tgtggttttt acccttcccg gtgctttcct tcctgaggcc agggctactt cttcctcctc
     7561 acgttcaagc atccttatta cctcttcact gttcctggca gtatcgagaa caacctgctg
     7621 gctatccgga acatctggta ttgttatttt tactacaggc ttttccatac ctgttatcct
     7681 ggtgttgaca ggatgcagaa gataatatag cacctctctg ctcgcaacga aaagtgctga
     7741 ttgacgaagc tgattttcaa acctcacatc accggaagtg tgcattccct taagcaacag
     7801 aagatgagca ctctgaaacc acggatatat ccccaccagc tctctaagct cagcagtaac
     7861 accctggtta atctttccgg gatcctttaa aagattcaga aaatcagacc tgttcatctt
     7921 cttttaccag ttaacgaatg ctgcattaaa aatatcctca acgagcaatg caattatttt
     7981 ttccgaaaga tccttttcaa catcactcag attaagtctg ctatcataat cttcgtatct
     8041 cgaaaacgtc ttttcaaaat tatattgagg ttcaacacta ttagtaaatt taaccttcac
     8101 cgtaatggta aacctgttca ttgccgctct tgaatcggct ccgacggtta gcggccgggt
     8161 attgtaatct gttatttctc cttcaaaatt gacatcaccg aagccgttca caaggctcag
     8221 ccgggtttgt gccctgcatt tgtcaataag tgcgtcggtc aatgcctgac tgagaccggg
     8281 ctgaacaaga ctcgccctgt tctgaaaata ttgtacactc agagtcttta cctgtggtga
     8341 aatactggcg cctgaaaaag aatatgagac cctgcagctg ctggctgaaa aaaatagcag
     8401 aacgattagt acagaggcat agatgcgttt aatcaggaaa tatttacatg ttaataccat
     8461 actcttttat tttgcggtac aatgtccgtt cggatatacc cagctccttt gcagcctgtt
     8521 ttcgcttgcg gttatgcttt tcaagtgctt tcacaattag ttctgcttct ttttcagaaa
     8581 gagacaatga ctcttccaca atctcttctg tatcctgaat atcagctgaa tgtcttgatt
     8641 taaaatcaac tgcagtcccc tgaacagaag tagctggagt tgattcgatc tttgcttctc
     8701 cgtagaggtt tctgattact cctgtatgtt cctgcttgaa attcatatct ccttcctttt
     8761 caatcattcc aagtaccagt ttcttaaggt cattaacatc atttctcata tcaaacagaa
     8821 tcttatatag tatttccctc tcggatgaaa aagatttctc atcgggttgc ctcatcacaa
     8881 caggcaactt cactgctgaa taatctggaa gatagcggcg cagaatctct tccgttattt
     8941 ctctttcatt ttcaataatt gaaatctgtt cggtaatgtt tttaagctgc cttacgttgc
     9001 cgggccagtc ataactgagc agcatagcct gcgccccggg ggtaagacga ataggtggca
     9061 tccggtaacg ttctgcaaaa tcggcagcaa actttctgaa aagaagaata atatcttccc
     9121 ccctttccct gagagccgga atcttaatag gaactgtatt aagcctgtaa aacagatcct
     9181 ctctgaattt gccttcagca atcagaaccg gcaaatcaac atttgttgca gcaataaccc
     9241 taacgtttgt cttctgcact ttggaagaac ccacacggat aaattcacct gtttcaagaa
     9301 cccttagaag tcgaacctgg gttgagagcg ggagttcact cacctcatcc agaaatattg
     9361 ttccaccgtc ggcaacctca aaatatccct tgcggctgtc agtggctccg gtaaaagctc
     9421 ccttttcatg cccgaacagt tccgaatcaa tagttccttc aggtattgcc ccacagttta
     9481 caggtatata ctgcccgtgc tttctggtac tgtaactatg aataacctgt ggaaaaatct
     9541 ctttacctgt tccactttca cctgtaataa gtacagaaag gtcggtagga gctacctgaa
     9601 ctgctatgtc gagggcccgg ttaagaccgg gatgattccc tattatccca aatctctgtt
     9661 taatgctttg cagatctctc attattaatt ttaataatgt gcaaatttac taatttttaa
     9721 aacagaggtg aagttccttt ttcttaaatt agcggcatga aagttcagga aaaattgcat
     9781 attacaggaa taatacctgc caggtatggt tcaacccgtt ttccaggcaa acctcttgca
     9841 actattggca ataagccaat aataaggctt gtatacgaac aggctttgaa ggcaatagaa
     9901 aatgtttatg ttgccactga tgacgaaaga attctgaaag ctgtcgtgga atttggcggc
     9961 aaagcagtta tgacatcaac cctgcatcgc agcggcactg accgatgcgc cgaagcagta
    10021 aaaataatcg aggaaactac aggcaggagc acagatgtta tcataaatat ccagggtgac
    10081 gagccctttg taaaaccgga acatataagc ctgcttgcct catgcttcga tgatccatcg
    10141 gtggaaatag caacactaat acgaagggta aaacagggag aaaacatctt cgacgaaaac
    10201 catcccaaag ttgttattaa tattttcggc gacgcaatct attttagcag gtctgtaata
    10261 ccattcatta gagatgccgg gaaacctgag tggaatctga agcacaatta tttcaagcac
    10321 ataggaattt atgccttcag gaaagagacc cttttcaaag taacctccct tgaaccaagc
    10381 tcactggaaa ttgcggaatc ccttgaacaa aaccggtgga tagaaaacgg cttaaaaata
    10441 agaaccgcag tggtagactg ggagagcatt agcgtggata ctcctgcaga tcttgaagtt
    10501 gcaagaagat tctatgaaaa aatgcgcctt gaggattaat ccaggtgaaa cttttgcaca
    10561 gtttttgcag tataaatgtt tgaagcagat aaataatgaa aagcctgttt ttaattctgg
    10621 ccgctttcgt tataagttcc tccctatttg cccagaagga ctcaacggca cttgcactga
    10681 agagtagcaa taagctatct gcatcagata attccacatc agtaagcatt tatccggtac
    10741 ctgtaaaaga cgggcagttt acaataaaaa gcattaagga aatctcatcg gtgagaatta
    10801 caaatattat aggacaggaa atatttaaag ccaattataa caaccccgaa aaagagattc
    10861 aggtttccct cgacaatccc aacaggggaa tgtacattgt agttattaca ttcacagaca
    10921 gaacacgcct ggttaaaaaa ataacaacag aaggcactca gtagtggcgc taaaagcagt
    10981 cgaattcaat ttatctcaga aatttattac ccctcaatca ggagattgaa atattttttc
    11041 tcttcactga aatctgcctc cgggactctt ttctcaaact gcgaaatttt cctgagagtt
    11101 ctggcagcga aatcttttac agctgcagga ttggttgaaa tgaacctgtg atttctggct
    11161 tttactattt ccattgcttc attgaaaatc gaaaaagttt ccttttcaaa aaaagcatcc
    11221 atgaaagaat taaaaatata atctattgcc gtttcagatg gatgaatcat atcggcagca
    11281 taaaatctat agtcacgcag gtcgtccata agcagttcat atgccggaaa ataccccggg
    11341 gatattttat ggcccagaag tttctcaatt gcaagaaaaa gtattgattt gcttacctgg
    11401 ttgccatgtg ctccttcttt cagatacctt accggactta ctgtgaaaat gacacttagt
    11461 tcagggttaa aagccgcaat acggtcgagc agtttttccc ataattcgca tatcccctca
    11521 acctccagca gttcgttgct gaacatcgtt gaaggcaact tatgacagtt cgaaacaacc
    11581 atccccccct ccttcagttt gtaaacccgg gcggtcccga aagtgacaaa cagaaattga
    11641 gcttgcttca gaaaatcatg tgctttcgcg tttgaatcat taattttctc aacggcatga
    11701 tcgggatcga cagaagaaaa attagtataa tggtaaaaac tgtgataaag attgtcataa
    11761 taaaaaaggt catttttagt aaaaacccgc cccgacataa tcatatcaag agtattggca
    11821 atggaaacag ggttatagat tgttccgtat gg
//
LOCUS       DAOSNB010000041        11543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5573_length_11543_cov_4.511403, whole genome shotgun sequence.
ACCESSION   DAOSNB010000041 DAOSNB010000000
VERSION     DAOSNB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5573_length_11543_cov_4.511403"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="PLG42_03135"
     CDS             <1..1866
                     /locus_tag="PLG42_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU30239.1"
                     /translation="LWIKASGYPLGTLTEEGLVALDREKLNIISLREYSDDPLKREEE
                     IKSDLSAAVYEEYRGKRPSVETSLHNLIRYRFVVHLHPAVINGVLCSRNAKNLCTKLF
                     GNEALFVHYTDPGYTLFKKLEDEINLWREKNDNDPKIILIENHGSFVSADSVEEIHNI
                     YDKIIRKISSIYEPLPDTTPLPFNPVLEKVFPAIRMILSEKRPKVIRHRYNALIDMFS
                     TSQQQFQKISHPLTPDMIVYCKPRYIYIEQSSSAERILESFKNQLERFRNEYGFDPRV
                     IVIKGMGVVAVEDNYFAAEQVLDVYEDLVKVSFYASFCGGIKLLSPEQVDFIDRWEVE
                     NYRRKVSRGSEEEYKLENKIAIVTGAAQGFGAGIAEALFRLGMNIVVADINEEKGRLF
                     AETLNKNNTKNRSFFVKVDVSDPGSVKKMVYTAVGEFGGLDLMISNAAILRAGSLEEM
                     DPDTFKKMTETNYNGFFYCTKYASEVMKIQNREKPEYFTDIIQINSKSGLKGSNKNFA
                     YAGAKFGGIGLTQSFALELAPFRIKVNSICPGNFFDGPLWSDPVTGLFVQYLKTGKVP
                     GAKSIEDVRRYYEEQVPLGRGCRVEDVVKAILYVIDQEYETGQAIPVTGGQIMLK"
     gene            1937..3199
                     /locus_tag="PLG42_03140"
     CDS             1937..3199
                     /locus_tag="PLG42_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOU30240.1"
                     /translation="MKTKAVRIYGKRDLRLEEFELPPLQDDEILAQVISDSICMSSHK
                     AALQGADHKRVPDDVAVNPTIIGHEFAGVILEVGKKWRHKFSPGQKFSIQPAMAHMGT
                     LDAPGYSYRYIGGDATYIIIPSIVMEAGCLLPYEGDAFYAASLAEPMSCIIGAFHASF
                     HVPPGTYIHEMGIRPGGKMAILAGTGPMGLGAIDYALHCERKPSLLVVTDIDEKRLAR
                     AQSLFSPEKAEKEGTRLVYVNTGKIDSPVMYLKNLTGGTGFDDVFVFAPVRQVIEQAD
                     AILGFDGCLNFFAGPADQSFSAEINFYNVHYAFTHIVGTSGGNTDDMREALRLISEGK
                     INPAVMITHVGGLDSVIETTLNLPNIPGGKKLIYTNISMPLVSLYQLEELGRTDNFYK
                     GLHEIVARNNYIWSPEAERYLLANSKPI"
     gene            complement(3142..4080)
                     /locus_tag="PLG42_03145"
     CDS             complement(3142..4080)
                     /locus_tag="PLG42_03145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOU30241.1"
                     /translation="MRRIFAVGEALVDIIFSGGEPRAAKAGGAMLNTSVSLGRAGLPV
                     SFISEFGADDPGRLIDSFLRSNGVDTSYVYRFTRGNTALALAFLDENNNAHYTFYKNY
                     PSQRLNTELPVIGKDDIFLFGSIYAITEEVRSSVMKLLRSASENNALVIYDPNFRSSH
                     LHELGKLKPLIMENINISTVVRGSHEDFQNILGLENADEVWKTLKEFSKCIIYTNSTE
                     GVYVRTPSFSGEFPVKKIEPVSTIGAGDNFNAGLIFAFYNGNIKPSDILELDYREWEK
                     IIATAVDFATEVCLSWDNYIGFEFASRYRSASGLQI"
     gene            complement(4089..4577)
                     /locus_tag="PLG42_03150"
     CDS             complement(4089..4577)
                     /locus_tag="PLG42_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30242.1"
                     /translation="MENLQEDKKIELGKDALSYLDTTRKWTMFFAILGFVFIGLMLLG
                     CVIAGVLLNSLPGLSEFESVDDMESVGLMAGAARIMLIVFMVIFAIIYFFPFYYLLRF
                     SQHTSRAIKKLSSEELTMALRNLKAYWLYIGVLVIIVLSVYLLIFIIAGTSLAFLSRI
                     FG"
     gene            complement(4841..6601)
                     /locus_tag="PLG42_03155"
     CDS             complement(4841..6601)
                     /locus_tag="PLG42_03155"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOU30243.1"
                     /translation="MGDFPKVGIRPAIDGRMMGVREALEKQTMNMAKNAAKLISSNLR
                     YPDGSPVECVIADTCIGGVAEAAMCAEKFRKQGVGVSLTVTPCWCYGTETIDMDSLIP
                     KAIWGFNGTERPGAVYLAAALAGHSMKGLPAFGIYGRDVQDAGDETIPEDVKEKILRF
                     VKAGLSVAYMKGKSYLSMGSVSMGIAGSIVREDFFQDYLGMRNEYVDMSEFIRRMNLE
                     IYDREEFRRALAWTKKYCKEGKDLNKKPRTREQKDKDWETVVKMTIIARDLMVGNPRL
                     KDLGFGEEALGHNAILAGFQGQRQWTDFMPNGDFLEAILCSSFDWNGIRPPYLVATEN
                     DAMNGVPMLFGYLLTNTAQMFSDIRTYWSPDAIKRVTGKKPTGLAENGIIHLINSGAS
                     SLDFTGRQTKGGKSVIKPFWEITEKDVEACLKATVWPPANTGYFRGGGFSSQFDTKGV
                     MPITIMRVNLIKGLGPVLQLAEGYTVELPPDMHKILTQRTDPTWPTTWFAPRLTGKGP
                     FVDVYSVMANWGANHCASSYGHIGADVITLASMLRIPVCMHNVPEEKIYRPSTWNAFG
                     MEKEGADYRACMTYGPLYKG"
     gene            6855..7577
                     /locus_tag="PLG42_03160"
     CDS             6855..7577
                     /locus_tag="PLG42_03160"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOU30244.1"
                     /translation="MKISKSLIITALLLILTSCVDGQFYRTVRGNGNVVKKERPASYF
                     DGISVSTGIDVYLSQGDGEKIIVEADENLHDYLVTEVKDRTLRIYFDDVSIRSAERKR
                     VYVTMKDVTKLKTSSAGDIIGETPVKCGDILLESSSAGNIRLELYARNANVKISSSGD
                     ISLTGEAETLNADLSSAGDLNAWDFKVKDADIDVSSAGDADIYVTHKLVARVSSAGDV
                     RYAGEPEIIDVRTSSAGTVRKR"
     gene            7686..9032
                     /locus_tag="PLG42_03165"
     CDS             7686..9032
                     /locus_tag="PLG42_03165"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOU30245.1"
                     /translation="MKENINVNVNGVSDFISLEEIAAHSKLAVRHLETLLSGTGPGND
                     FLGWLRLPENAGTQTARIEKIAKHLRSVSNTTVVVGIGGSYLGARAVIEALSHSFPHH
                     QKDKNHEIIYAGHNVCEEYMADLLEVLEHRNYSVIVISKSGTTMEPAIAFRLIKDHVE
                     RKFGKSVAADRIVAITDATKGALRSLAELNGYETFEIPDDIGGRYSVLTPVGLLPIAV
                     AGIDIRELIEGAKAAAAITRDNKDPQKNPALIYAATRNLLLQAGKPLEIMVNFHPKLH
                     YFAEWWKQLFGESEGKEGKGIFPASVDFTTDLHSLGQYIQEGQRILFETVLSIKSSPA
                     KVKIPVEKDDQDQLNYLAGKQLGEINKSAEIGTIIAHTEGGVPVISVNIPSLDAYYLG
                     QLIYFFEISCAISGYILDVNPFDQPGVEAYKKNMFALLNKPGYEEAGKKLREKLKQ"
     gene            9050..10129
                     /locus_tag="PLG42_03170"
     CDS             9050..10129
                     /locus_tag="PLG42_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOU30246.1"
                     /translation="MGLELRKNFMWSLVVPTSMGVRITPVNFQPVHCSDTYVMQATSA
                     ESNVASVSSYLGLPVKVLTTFVKDSPIARFIKDNLASRHIVWEGVEVEQGGPWGYRHQ
                     FNIADTGYGPRGPRVHNDRAGEVGRTLNVKDFDLERLFAKEGVQILHMSGLIAALSPD
                     TSNFCLELARKAKKHGTLVSFDLNYRASFWKNREKELSAAFSEIASLSDILVGNEEDF
                     QLSLGIEGPEAGGKDIASKIESFKEMISRVRKSYPGAKVFATTLREVINANRHLWGAI
                     MLEGETWHVIEPREIFVLDRIGGGDGFVGGLLYAILKGWEPEKWIQFGWAAGAMATTF
                     MTDYCQPADEEQIWSIWKGNARVRR"
     gene            complement(10119..11462)
                     /locus_tag="PLG42_03175"
     CDS             complement(10119..11462)
                     /locus_tag="PLG42_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOU30247.1"
                     /translation="MAVKNVIIIGAAGRDFHNFNVYFRDNEYYRVVAFTAAQIPDIEG
                     RKYPAGLAGRLYPEGIPVYPEEELPRLIRETKTDICVFSYSDVPYQKVMSLSAIVNAA
                     GASFMLLGPAETMKRSNKPVIAVGAVRTGCGKSQTSRRVIEILMSKGLKVVAVRHPMP
                     YGDLESQKVQRFAEIKDLEKYNCTVEEMEEYEPHIVRGNVIYAGVDYEAILKEAENDP
                     QGCDVIVWDGGNNDFPFFVPDLMITVTDPHRAGHELRYYPGEVNLRIADVVIINKIDT
                     ASPDAVQIVRENISKVNPGAVVIDAASPVKTENPSVIRGRRVLVVEDGPTLTHGEMKI
                     GAGVIAARRYGASQLVDPRPFIVGKLVETFRTYPEIGTVLPAMGYGRQQLRDLERTIN
                     NTDCDSVIIATPIDLNRIINIQKPTTRVYYDLQEIGYPDLTQVLDEFLERKKIIS"
BASE COUNT         3380 a         2646 c         2425 g         3092 t
ORIGIN      
        1 ctatggatta aagcaagtgg ttatccactt ggcaccctta cagaagaggg acttgttgct
       61 cttgaccgtg agaaactcaa tattatctcc ctcagagaat attccgatga tccactaaaa
      121 agggaagaag agataaaaag tgacctttca gcagcagttt atgaggaata cagagggaaa
      181 agaccgtcgg ttgaaacatc actgcataat ttaataagat acaggtttgt agttcatctt
      241 cacccggcag ttattaacgg ggtgctttgc agcagaaatg caaaaaacct gtgtacaaaa
      301 ctctttggaa atgaagctct gtttgttcac tataccgatc cgggttatac gcttttcaaa
      361 aagcttgaag atgagataaa cctgtggaga gagaaaaacg ataacgaccc gaaaataatc
      421 ctcatcgaaa accatggaag ttttgtaagt gctgatagtg ttgaggaaat acataatatt
      481 tacgacaaga taatacgtaa aatatcatct atctacgaac cattgcctga caccactcca
      541 ttacctttta atcctgtgct ggagaaagtc tttcctgcaa taagaatgat attatccgaa
      601 aagaggccga aagtcatcag gcaccggtat aatgctctga tagatatgtt cagcaccagc
      661 cagcagcagt tccagaagat ttctcacccg cttacacctg atatgattgt gtattgcaaa
      721 cccaggtata tttacataga acaatcatca tctgccgaaa ggatacttga atctttcaaa
      781 aatcaactcg aaaggttcag aaacgaatat ggatttgacc ccagggtgat agttatcaaa
      841 ggaatgggtg ttgttgctgt ggaggacaac tactttgctg cagagcaggt tcttgatgtt
      901 tacgaagacc tcgtaaaagt aagtttttat gcctccttct gcggggggat taaacttctc
      961 tcgccagaac aggttgactt tattgacaga tgggaagttg aaaactaccg cagaaaagtt
     1021 tccagaggtt ctgaagaaga atacaaactg gagaataaga ttgcaattgt cacaggagca
     1081 gcacaggggt ttggtgccgg tattgccgaa gccctattcc ggctgggcat gaacatagtt
     1141 gttgctgaca tcaatgagga gaaaggcagg ttatttgccg aaacactcaa taaaaataac
     1201 acaaaaaacc ggtcattttt cgtaaaagta gatgtttcgg accccggctc ggtaaaaaaa
     1261 atggtttaca ctgcagttgg ggaattcggt gggctggacc tcatgataag caatgctgca
     1321 atactcaggg ctggctcgct ggaagagatg gacccggata cgttcaaaaa gatgactgaa
     1381 accaactata acggcttctt ttactgcaca aaatatgcct cggaagtaat gaagatacag
     1441 aacagagaaa aacctgaata ctttactgac ataatacaga taaattctaa atcggggctt
     1501 aaaggcagta ataagaactt tgcatatgca ggtgccaaat tcggaggtat agggcttaca
     1561 cagagctttg cccttgaact ggctcccttc agaattaaag ttaacagtat ttgtccgggt
     1621 aatttcttcg acgggcctct gtggtccgat ccggttaccg ggctctttgt ccagtatctg
     1681 aaaacaggaa aagtgcctgg cgctaagagc attgaagacg taaggcgtta ctacgaagag
     1741 caggtcccgc ttggacgcgg ttgcagggtc gaagatgttg taaaagctat actttatgtg
     1801 attgatcagg aatatgagac tggtcaggca atacctgtaa ccggcggaca gattatgctg
     1861 aaataacctt tatcaccagc cccattcccc ttaaataaac tggagaatta acttattttt
     1921 taataaaata tcagagatga aaaccaaggc tgtcaggata tacggtaaaa gagatttaag
     1981 acttgaggaa tttgagctgc cgccccttca ggatgatgag atacttgcgc aggttatatc
     2041 cgacagtata tgcatgtcgt cgcacaaggc agcacttcag ggggccgacc acaagcgtgt
     2101 acccgacgat gtagctgtga acccgaccat aatcggacac gaatttgccg gtgttatcct
     2161 tgaagttggc aaaaagtggc gtcataaatt ttctcccggc cagaaattct ccatacagcc
     2221 tgctatggct cacatgggga cactcgatgc acctggttat tcgtaccgct atatcggagg
     2281 tgacgcaaca tatattatca ttcccagcat tgtaatggag gctggttgcc tcctgcctta
     2341 cgaaggtgat gcattctacg cagcctccct tgccgaaccg atgtcgtgca tcataggagc
     2401 attccacgcc agcttccacg taccacccgg aacctatata catgagatgg gcatcagacc
     2461 aggaggcaaa atggcaatac tcgcaggaac aggcccgatg ggactcggtg ccatcgacta
     2521 tgcactccac tgcgaaagaa aaccttccct gctagtggta accgatatcg acgagaaaag
     2581 gcttgcacgt gcacaatcac tcttttctcc tgaaaaggca gaaaaagagg gaacccggct
     2641 cgtttatgtc aacaccggaa agattgattc accagttatg tatctcaaaa accttacagg
     2701 aggtactggt tttgatgatg tttttgtgtt tgcaccagta aggcaggtaa tcgagcaggc
     2761 cgatgccata ctaggtttcg acggatgtct caattttttt gccggaccgg ccgaccagag
     2821 tttcagtgca gaaataaact tctataatgt tcattatgcc tttacccata ttgttggaac
     2881 ttctggcgga aataccgacg atatgcgcga agctttaagg ttaataagcg aaggaaaaat
     2941 taatcctgcc gttatgataa cacatgttgg aggcctcgac tcggtaatcg aaacaactct
     3001 taatcttccc aacattccag gaggtaaaaa actaatctac acaaacatct ccatgccgct
     3061 tgtttcactc taccagcttg aagaactggg aagaaccgac aacttttaca agggcctcca
     3121 tgaaattgtt gcccgcaaca attatatctg gagcccagaa gcggagcgat acctgctggc
     3181 aaactcaaaa cctatatagt tatcccaact caaacatacc tctgtggcaa aatcaactgc
     3241 agtagcaata atcttttccc actccctgta gtcaagctcc aaaatatctg atggtttaat
     3301 atttccgttg taaaatgcaa atattagtcc tgcattgaaa ttatcccctg ctcctatggt
     3361 gcttacaggc tcgatctttt ttaccggaaa ttctcccgaa aatgaaggtg tccttacata
     3421 aacaccttca gtgctattag tgtaaataat acacttacta aattccttca atgttttcca
     3481 aacctcatct gcattttcca aaccgagtat attttggaaa tcttcatgtg agccacgcac
     3541 tacagtggaa atatttatat tttccattat caggggttta agctttccga gctcatgaag
     3601 atgggagctt ctgaaattgg gatcataaat tactagagca ttgttttccg aagcactcct
     3661 cagcagcttc atcacgctgc ttctaacctc ttctgtaata gcatatatag acccaaacag
     3721 aaagatatca tctttcccta ttacgggaag ttcagtattg aggcgctgtg agggataatt
     3781 tttataaaag gtataatgtg cattgttgtt ttcatcgaga aatgcaagtg ccagcgcggt
     3841 gtttcctctg gtaaacctgt aaacgtatga tgtatcgacg ccattacttc tcaaaaaact
     3901 gtcaatcaat ctcccgggat cgtcggcacc gaactcacta ataaatgata ccggtaaccc
     3961 tgccctcccg agtgaaacag aagtattaag catggcaccg cctgcttttg cagcacgggg
     4021 ttccccgcca ctgaaaataa tgtcaacaag tgcttcacca acagcaaaaa tcctcctcat
     4081 cactcagatc atccgaaaat acgggaaaga aatgcaagtg aagtgcctgc aataataaag
     4141 attagcagat ataccgacaa aactattatt accagcactc cgatatatag ccagtaagct
     4201 tttaggtttc ttaaagccat tgttaattcc tccgagctta gcttctttat tgctctcgat
     4261 gtgtgctgcg aaaaccttaa gagataataa aacgggaaaa aataaattat ggcaaaaatg
     4321 accatgaaaa caataagcat tatccttgct gcacctgcca ttaaaccgac actttccata
     4381 tcatccacac tctcaaattc acttaagcca ggtaatgaat taagcaacac accggcaatt
     4441 acacaaccca acagcatcaa cccgataaag acaaacccga gaattgcaaa aaacatggtc
     4501 cattttcttg ttgtatcgag atagctcaag gcatctttcc ccagttctat ctttttatct
     4561 tcctgtaaat tttccattat taagcattta atttatccaa atcatttttc cttaaaaaaa
     4621 ttcagtatct gtatcaatct cataccttaa acttccccaa ctttaacctt ttaaccttaa
     4681 accccgaacc tcgaaacatt gaacccttaa cccttaaacc cttgaaccag caaacccgtt
     4741 aacccttgaa ccagcaaacc cgttaccagc aaaccagcaa acccgtgaac cagtgaaccc
     4801 gtaaacccgt taaccagcaa acccgttaac cagtgaaacc ctaaccctta tacaaaggcc
     4861 cataagtcat acatgccctg taatctgctc cttccttttc cattccgaaa gcgttccagg
     4921 tactaggacg gtaaattttt tcctcaggca cattatgcat gcacacaggt attcttagca
     4981 ttgaggcaag tgttatgaca tcagctccga tatgtccata gctcgatgcg cagtggtttg
     5041 ctccccagtt tgccattact gaatatacgt cgacaaacgg acctttgcct gtgaggcgtg
     5101 gtgcaaacca tgtcgtcggc catgttgggt cggttctctg ggtcagaatc ttatgcatat
     5161 cgggaggcag ttcaactgta tatccttctg ccagttgcaa gacaggtcca agtcctttta
     5221 tcaggtttac tctcattatt gtgattggca tcacgccttt tgtatcgaac tgtgatgaga
     5281 agcctccgcc cctgaagtat ccggtatttg ccgggggcca tacggttgcc ttcaggcagg
     5341 cttcaacatc cttttcagtt atttcccaga atggtttgat gacactctta ccacctttag
     5401 tctggcgtcc tgtgaaatca agcgatgaag ccccggaatt tattagatgg attatcccat
     5461 tttcagccaa ccctgtcggt ttcttacctg taacccgttt tatagcatca gggctccagt
     5521 aagtgcgtat atctgaaaac atctgggcag tattggtaag aagataaccg aatagcattg
     5581 gaacgccgtt catggcatcg ttttcagttg caacaaggta gggaggtctt atgccgttcc
     5641 agtcgaatga tgagcagaga atcgcttcaa gaaaatcacc gttaggcatg aaatcagtcc
     5701 attgacgctg accctggaaa cctgcgagaa tggcattatg accgagtgct tcctcaccga
     5761 agcctaaatc tttcaacctt ggattcccta ccatcagatc gcgggctata atggtcatct
     5821 taactacagt ttcccagtct ttgtccttct gttcacgcgt tctaggcttt ttgttcaggt
     5881 cttttccttc tttacaatat ttctttgtcc atgcaagtgc cctcctgaat tcttccctgt
     5941 cgtatatctc aagattcatc ctccggataa attcagacat atcaacatat tcattccgca
     6001 ttccgaggta atcctgaaag aaatcttccc tcactattga accggctatg cccatggaga
     6061 cagaacccat tgagaggtat gatttccctt tcatgtaagc aactgaaagc ccggctttta
     6121 caaagcgaag tatcttttcc ttcacatcct cggggatagt ctcatcacct gcatcctgca
     6181 catcacggcc ataaattccg aaggctggaa gtcccttcat gctatggccg gcaagagctg
     6241 cggcaaggta aaccgcaccc gggcgctcgg tgccgttaaa cccccatatt gccttcggta
     6301 tcagcgagtc catgtcaatt gtttccgtgc cgtaacacca gcagggcgtc acagtgagac
     6361 tgacacccac accctgtttg cgaaacttct cagcacacat tgctgcctct gctacaccac
     6421 caatgcatgt gtcggcaata acacattcaa caggcgagcc atccggatac ctcagattcg
     6481 atgaaatcaa ctttgccgca ttctttgcca tgttcatggt ctgcttctca agagcttccc
     6541 gtacacccat cattcgccca tcaatagcag gacgaatgcc tactttcgga aaatcaccga
     6601 taagacgtat atatcccgaa ctcttttttg ttttctctat cgccataaca agatattttt
     6661 actggtttta acttttaaca aatataattt taataattca attgtttatt tttctgaata
     6721 aattattgag tttcattata cctgttattt gtgctgtaga gattcattga agaaaattat
     6781 tttatatcct gcacgcatca aaatgaaaaa tataacgtct ttattgtgaa tttaacaaca
     6841 aaaacctgaa tcagatgaaa atcagtaaat ctttaattat taccgcactg ttacttatat
     6901 tgacttcctg tgttgacggg cagttttaca ggacagtacg gggtaacggc aacgtggtaa
     6961 aaaaagaaag gcctgcttcc tacttcgatg gtataagtgt cagcaccggc atcgatgtgt
     7021 atctcagtca gggtgacggg gaaaaaatta tcgttgaagc cgatgaaaat cttcacgatt
     7081 acctcgttac agaagtaaaa gacagaactc tcagaatata tttcgacgat gtaagcatcc
     7141 gcagtgccga aagaaagaga gtctatgtaa ccatgaaaga tgtgacgaag ctcaaaacaa
     7201 gcagcgcggg tgatattata ggggagacgc ccgtaaaatg cggggatatt cttcttgaat
     7261 ccagcagtgc cggaaacatc aggcttgaac tttatgcccg taatgctaat gttaagataa
     7321 gcagttcagg cgatatcagc ctcaccggtg aagcagagac tcttaatgct gacctcagca
     7381 gtgcgggtga tcttaatgca tgggacttca aagtaaaaga tgcagatata gatgtatcca
     7441 gtgccgggga tgccgacatt tatgtaactc acaaactggt ggcacgtgta tcaagtgccg
     7501 gggatgtcag atatgctgga gagcctgaaa ttattgatgt cagaacttca agtgcaggga
     7561 cagtccgtaa aagataaaca gtagcagaaa atttattttt atgtgcaagc tgccggagca
     7621 attccggcgg ctttttttat ctttgtacag caaggagccg cttaaaacaa cttaaaatat
     7681 ttaaaatgaa agagaacatt aatgttaacg taaacggagt gtcagacttt atctcccttg
     7741 aagagatagc tgctcactca aagctggcgg tcaggcacct tgaaactctt ctttcgggaa
     7801 ccggaccggg caacgatttc cttggctggc ttagactgcc agaaaatgca ggcacgcaga
     7861 cagcaagaat agaaaagata gcgaagcacc tgcggtcagt ttcaaacact actgttgttg
     7921 taggtatagg aggctcatac ctgggtgcca gagctgttat tgaagctctt tcacactctt
     7981 ttccacacca tcagaaagat aaaaaccacg aaataatata tgcaggtcat aatgtttgcg
     8041 aggagtatat ggccgatctt cttgaagtac tcgaacatcg aaattattcc gttatagtga
     8101 tctcgaagtc gggaaccaca atggagccgg ctattgcttt caggcttatc aaagaccatg
     8161 ttgaaaggaa gttcggcaaa tcagtagcag ccgaccgcat agttgcaatc actgatgcaa
     8221 caaaaggcgc actgagatca ctggccgagc ttaacggtta cgaaaccttc gaaatacccg
     8281 atgatattgg aggtcgctat tcagttctta ctccagtagg cctgttgcca atagcggttg
     8341 caggaataga tatccgtgaa cttattgaag gggcaaaggc tgctgcggct attacccgcg
     8401 acaacaaaga tcctcagaaa aatccagcac ttatatacgc agctaccaga aatctgctgc
     8461 ttcaagctgg taaacctctc gagattatgg tgaacttcca tcccaaattg cactattttg
     8521 ccgaatggtg gaaacaatta ttcggagaaa gtgaaggaaa agagggcaaa ggcatattcc
     8581 ctgcttcagt cgacttcaca actgacctcc attcactggg acagtacata caggaaggac
     8641 agaggatact ttttgaaacc gtgctttcca taaaatcgtc acctgcaaag gttaaaatac
     8701 ctgtggaaaa agacgatcag gaccagctga actatcttgc agggaaacaa cttggtgaga
     8761 tcaacaaaag cgctgagata ggaaccatta ttgcccacac cgaaggaggg gttccggtga
     8821 tatcggttaa cataccttcc cttgatgcat attatctagg acagttgata tacttctttg
     8881 agatctcatg tgccataagc ggatacattc ttgatgtgaa tcccttcgat cagcctggag
     8941 ttgaggctta caagaaaaac atgtttgccc tgctgaataa accaggctat gaggaagcag
     9001 gcaaaaaact cagggaaaaa ctaaaacaat aacatataaa ttttgaatta tgggtctgga
     9061 acttagaaag aacttcatgt ggtcgctggt tgtgccaacc agcatgggtg ttcgaattac
     9121 gcccgtaaat tttcaacctg tgcattgcag tgatacatac gttatgcagg caacgagtgc
     9181 cgaatcgaat gttgcaagtg tatcatcgta cctggggtta ccggtaaagg tgctcactac
     9241 atttgttaag gatagtccca ttgcaaggtt cattaaggac aaccttgcaa gcaggcatat
     9301 tgtatgggaa ggtgtcgaag ttgagcaggg aggcccgtgg ggataccgcc atcagttcaa
     9361 catagctgat acgggttatg gacctcgagg cccaagagtc cacaatgacc gtgccggcga
     9421 ggtgggcaga acacttaacg taaaagattt tgacctcgaa agactctttg cgaaagaagg
     9481 agttcagata ctacatatgt cggggcttat agcagccctt tctccagata caagtaactt
     9541 ctgccttgag cttgcacgca aggcaaaaaa acatggcacg ctggtatcat tcgacctgaa
     9601 ttatagagct tcattctgga aaaaccgcga aaaggaactt tctgctgctt tcagcgagat
     9661 agcttcccta tccgacattc ttgttggtaa tgaagaagat tttcagctca gcctgggcat
     9721 tgaaggtccg gaagcaggcg gcaaagatat cgcatcaaaa atagagagct ttaaggaaat
     9781 gatttcacga gtacggaaaa gctatcctgg tgcgaaagtg tttgccacca ccctcaggga
     9841 ggtgataaat gctaacaggc acctgtgggg agctattatg cttgaaggtg aaacatggca
     9901 tgttatagaa ccgcgtgaga tttttgtgct cgaccgcatt ggtggtggcg acggcttcgt
     9961 aggtggatta ctctatgcta ttcttaaagg ctgggaacct gagaaatgga ttcagttcgg
    10021 atgggcagca ggagctatgg caacaacctt catgaccgac tactgccagc cagccgacga
    10081 agaacagata tggagcatct ggaaagggaa cgcccgcgtc aggagataat tttcttccgc
    10141 tccaaaaatt catcaagtac ctgtgtgaga tcgggatagc cgatctcctg caggtcgtaa
    10201 tagacccttg tggtgggctt ctgaatgtta ataatcctgt tcaggtcgat aggcgttgca
    10261 atgatcacgc tgtcgcagtc agtgttattg atagtcctct ccaggtcgcg cagttgttgt
    10321 ctcccgtaac ccatggccgg cagaacagtg cctatttcag gataggtcct gaatgtctca
    10381 acaagcttgc ctactatgaa aggcctcggg tcaacgagct gggaggctcc atatcttctg
    10441 gcggcaataa cacctgcacc tatcttcatc tcaccatgtg taagtgtcgg accatcctcc
    10501 actacaagaa ccctcctgcc tcttatgaca gaaggattct cagtcttcac aggcgatgct
    10561 gcatcaataa caacagcacc gggattaacc ttgcttatgt tttcccttac aatctgcact
    10621 gcatcgggtg aagcagtgtc tatcttgttt attatcacca catcggcaat tcttaaattt
    10681 acttcccctg gataatatct cagttcatgc cctgccctgt gaggatctgt aacagttatc
    10741 atcaaatcgg gtacaaaaaa cggaaagtcg ttattccctc catcccaaac aataacatca
    10801 catccctgag ggtcgttctc ggcttccttt aaaattgcct cataatcaac tccggcatag
    10861 attacattac ctcttacaat atgcggctca tactcctcca tctcctcaac ggtgcaatta
    10921 tatttctcga ggtcttttat ttcagcaaat ctctgaactt tttgtgattc aaggtcgccg
    10981 taaggcatgg gatggcgtac agctacaacc ttaagtccct tcgacataag aatctcgatc
    11041 accctccttg aggtctggct ctttccacat cctgttctta cagcccctac agctataaca
    11101 ggtttattac tccttttcat tgtttcagcc ggacccagca gcataaagct tgcacctgct
    11161 gcatttacaa tggcactaag ggacatcact ttctgatagg gcacatcact gtacgaaaaa
    11221 acacatatat cggtttttgt ctcccttatc agccggggta gttcttcctc cggataaact
    11281 ggaatgccct cgggatataa cctgcctgca agaccggcag gatatttcct tccctcaata
    11341 tccggaatct gtgccgctgt aaaggcaaca accctgtaat attcattatc cctgaaataa
    11401 acattgaagt tgtgaaaatc tcttcctgct gctcctataa tgatgacatt ctttacagcc
    11461 atattaatct tctattataa atatttttaa tccaccaaat ttaatacttc ccccaaggtt
    11521 actctaactt ttttaaaaaa tcc
//
LOCUS       DAOSNB010000042        11309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5732_length_11309_cov_4.479296, whole genome shotgun sequence.
ACCESSION   DAOSNB010000042 DAOSNB010000000
VERSION     DAOSNB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5732_length_11309_cov_4.479296"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLG42_03180"
     CDS             complement(<1..499)
                     /locus_tag="PLG42_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOU30248.1"
                     /translation="MAEINGHRQPSKIYTTGIILFFAALTFAAFNTSLPQEKKKQPGE
                     TKKIELLYCDVLIIDKQVSNDLNRLIGNVALKHKDIIMTCDSAHLYREINQVKAYGHV
                     KINQADTLTLAGDYLFYDGNEEKAFVQGNVELKDNESKLYTQVVNYDAKTKIADYPFH
                     GKIING"
     gene            complement(487..1842)
                     /locus_tag="PLG42_03185"
     CDS             complement(487..1842)
                     /locus_tag="PLG42_03185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU30249.1"
                     /translation="MKHKTVISLLMLFLVLNTGTLFSQKLVESVAGIVGNEVIFLSDI
                     ENAVAQQLWSGDRTPADRLRCNLFEEQLIAKLFLDQARIDSIEVSDSEVEGYLNERLN
                     KFIARAGSEKALEEVFRKSITEIRADLRKSLKEQEIVNRVQSKIAENITVTPAEVKKF
                     YSKIPPDSLPTIPAKVELSIIQLDPPDNELNKAEARQKLLDIRSEILAGKSFSTMAIL
                     YSEDKESAKRGGEIGYLSRAELEKPYADVAFSLTKNAISKIVETRYGFHIIQLIDRMG
                     DLVNTRHILIRPKIKPEQEERALSKLDSIANLIKADSITFERAAYLFSTHKDSKINGG
                     KLVKNDPAARTTLFTLEELDRNTYMVVRDLKPGEISKPFMTEDENGNTVFRIVRLDNQ
                     IPAHVADLKEDYQALYNAALLEKRQKRFLEWIDKKIEVTYIKISDEFKSCPFSNPLWL
                     K"
     gene            complement(1846..2703)
                     /locus_tag="PLG42_03190"
     CDS             complement(1846..2703)
                     /locus_tag="PLG42_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30250.1"
                     /translation="MRKALIYLSIITLSVSDISCLRQNKGPARIPVARAGEKILYYDQ
                     IPEIYRNTKSPEDSIVSINDYINSWARRELLYARAVENLSPEYKNDIESQLLDTKVNL
                     YIYHYQHQMMLEKMDTIVSDSEIEQFYASNPDRFKLNTSIVKALFIKIPAEAPNIDRV
                     RVWYRANDSESLRQLESYCFQFAEKFDDFGEEWISFDKLAVELPAEIINPDEFLKRYS
                     YYETRDSSSVYFIAIRDYRLRSSLAPYEYVKDDIKSIILNNRRFEFLKKLENDIYNDA
                     INSNIFKKY"
     gene            complement(2700..4658)
                     /locus_tag="PLG42_03195"
     CDS             complement(2700..4658)
                     /locus_tag="PLG42_03195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU30251.1"
                     /translation="MKYRLSIILMVIATGLLHAQGIQDSILLIAGGREVTAGEFMRMY
                     RKIYNPSDTGDFNNYLEQFIVFKLKVADAISKGYDTTKAFRTELQGYRDQVAKNYLTD
                     KNVKEEILKKSYEKTLKEINAWHILVSCPPNSSPEDTLAAFKKALEIRARIISGEPFE
                     QVARSASDDPSAIYNGGNLGYITTFQMITPFEEAVYSMKKGELSNPVRTPYGYHIIKV
                     ADIRPSRGKIKVAHIMRSVPPNAPEQVAKAAEDTIKYIYKKLLEGASFSDLAMKYSDH
                     KESAARGGELNWFGAGEIISEFAEAAFSLEKNGDFTPPVKTIYGWHIIKRLDKKDPPS
                     YEEARSQLESKIDETWLNSLATRSMVNSLKQEYKFRLNTTALNWFLENTDTLIIRGLS
                     KYDRGAIPSGTLYTFDGQKFSNVDFARYIESRASSNNTEEPANFVNKLAETCITDHIL
                     RYENSLLEKKYPEFRYLVNEFHDGILLFEISKEKIWDRAQNDTSGLKKYYEENKQRYP
                     GKKSFTGMIYVCMEKAKAGKFYKAARKYVKKPDFEKKLQAMFNAGSDTIIKVYKGVFQ
                     EGDSLLPENLKWEEGLHETEIKGFPSVIVITKINPASPLPLNEVMGEISSEYQNFLES
                     EWIKQLKEKYPVRINRTILEQVKNELQK"
     gene            complement(4677..6158)
                     /gene="guaB"
                     /locus_tag="PLG42_03200"
     CDS             complement(4677..6158)
                     /gene="guaB"
                     /locus_tag="PLG42_03200"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOU30252.1"
                     /translation="MSFIKDKILFEGLTFDDVLLIPAYSEVLPRDVDISSMFSRNIRI
                     NTPVVSAAMDTVTEAELAIAIAREGGIGVIHKNMSIEKQASQVRKVKRAENVMITDPV
                     TIHPDATVAEAISLMREYKIGGIPVTDKNGILKGIVTNRDLRFENNSSKKISEVMTTK
                     LITTTYQTNLEEAARILQKHKIEKLPIVDENNKLIGLMTYKDITKSKDRPNACKDEKG
                     RLRVAGAIGIAADTLERAEALINANVDALVIDTAHGHTKNVIGILRDIKRNFPNVDVV
                     AGNIGTEEAARRLVKEGADAVKVGIGPGSICTTRVIAGVGIPQLSAIYNVAKAIEGSG
                     VPVIADGGIRYSGDIIKAIAAGADSIMAGSLFAGVEESPGETIIYNGRKFKTYRGMGS
                     LEAMSQGSRDRYFQDVEDDIKKLVPEGIEARVPYKGTLAEVMYQMVGGLRAGMGYLGT
                     PDIPTLKKEAKFVRITGPGIIESHPHDVTITREAPNYSRKSVE"
     gene            6328..8238
                     /locus_tag="PLG42_03205"
     CDS             6328..8238
                     /locus_tag="PLG42_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOU30253.1"
                     /translation="MDIETCRKILTRYWGFTTFKPLQEEIIKSVLEKKDTLGLMPTGG
                     GKSVTFQVPGIALEGICLVITPLIALMKEQVARLNKMEIKALALHSGMTKEEIEITLD
                     NCIYGDYKFLYISPERIASPLFRIKSPRLNVSLVAIDEAHCISQWGYDFRPSYMKIVE
                     LRELLQTDPPFLALTASATPKVIDDIIEKLAFKTRNVLKTSFERKNISYIVRKVEDKS
                     SYLLNTLGKVKGSGIIYVRSRKKAREVAEMIASKGYSADYYHAGLPQELRDRKQAAWS
                     LDQVRIMVATNAFGMGIDKPDVRFVIHWDIPESIESYFQETGRAGRDNKPSWAVLLYS
                     DADISRLKESISHKFPPIEKIKDVYEMLCNYLRVPVGSGKDTVHDFNMQEFVAQYHLP
                     VIETYNCLAFLQREGYIEFTEEINNPSRVHFIVGRDDLYKFQVANESYDKFIKILLRS
                     YTGMFTEYVPVNEDALAKKAGMTRETVYQLLVRLSSMNILHYIPGKKTPLVIFTEERL
                     DRKTLFISPENYLHVKEKYLLRLEKMLEYAESQNRCRSVILLEYFGEEADRCGICDVC
                     RKRNELELSKYEFDLILEEIKAVLSVEKPDARELVRKINYPEEKVIKVVQWLLDHNKI
                     KQDTEMKLLWNG"
     gene            8349..8972
                     /locus_tag="PLG42_03210"
     CDS             8349..8972
                     /locus_tag="PLG42_03210"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOU30254.1"
                     /translation="MNKNQEPFYSGTVVEFVASANEYCKYMERAEELEGEELLKILQR
                     LLPFIYIKGSLLPELDPYFEEGNEKFVKEEDWNRIYETLKQKFGTADAFLEVFDERMK
                     ESEAPVVASLAENLADIYQDLKDFLILYQTGTNEIMNDAIWECRMNFENYWGQKLVNS
                     LRAIHRLVYSGEEIGKVPEEESEKEKMERLEKWIISQKQKEFRKNRN"
     gene            8974..9546
                     /locus_tag="PLG42_03215"
     CDS             8974..9546
                     /locus_tag="PLG42_03215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU30255.1"
                     /translation="MYLNNITPEELSNYRVGYFSGRIIVIDSMESFRLHSDFLTGRKI
                     LGFDTETKPSFRKGRRNRVSLLQLAYDNTALLFRLNKIGLPGELVKVLSDPGIIKVGV
                     AIHDDIRVLQDLKHFNPAGFVDLQKLVKEYGIESAGLKKIAAIVLGFRISKSQQVTDW
                     EAPVLSEAQKVYAATDAWVCYEVYRKLTGQ"
     gene            9565..10761
                     /locus_tag="PLG42_03220"
     CDS             9565..10761
                     /locus_tag="PLG42_03220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOU30256.1"
                     /translation="MIEGKQEYIRIVLKSGKDQSVRRYHPWIFSGAIKKIYGTPAEGD
                     IVEVYDNKGEFLATGHYQPGSIAVRILSFEKVIPDRDFFRKMISSAVEYRKQLGFLSD
                     PSTNVFRLIHAEGDNLPGLIADYYNGVVVLQMHSTGMYRIRNEIAEIISEILSEHVQA
                     VYDKSEATISFKSDIRPENGFISGSAEPSIILENGYKFYVDWITGQKTGFFIDQRENR
                     KLVQHYSCNRDVLNLFGYTGSFSVYAMKNARSVDTVDSSGPALKIADENIRLNYGEDS
                     RHHSYEADAFEFLAGCRNRYDLMIIDPPAFAKHNDVLANALQGYKRLNIKAIENIRPG
                     GIIFTFSCSQVVSKENFRKSVFAAAANTGRKVRILHQLGQPADHPVNIYHPESEYLKG
                     LVLYVE"
     gene            10819..>11309
                     /locus_tag="PLG42_03225"
     CDS             10819..>11309
                     /locus_tag="PLG42_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOU30257.1"
                     /translation="MLDKMPDMSVDKHFEFIANKLGLKVWQVENTIRLMDDGATIPFI
                     SRYRKESTGSLDEVTLLTIKDEYARLKELDKRREAIIRSIEEQGKMTPELRQRIDDAL
                     TLTELEDIYLPYRPKRRTRATIAREKGLEPLARLIYEQTEKDPVAKAAEFLNEQVANA
                     DEA"
BASE COUNT         3244 a         2218 c         2292 g         3555 t
ORIGIN      
        1 ctccgttgat tatcttgcca tggaaaggat aatcagcaat ttttgttttt gcatcataat
       61 ttacaacctg tgtataaagt tttgattcat tgtccttaag ttccacatta ccctgaacaa
      121 atgctttctc ttcattgcca tcataaaaaa ggtagtcacc tgcaagagtc aatgtatcag
      181 cctgattgat ttttacatgc ccgtatgctt tcacctgatt tatttcccgg tacagatggg
      241 cactgtcgca tgtcataatt atgtccttgt gtttgagagc gacatttcca ataagccggt
      301 taaggtcgtt gctgacctgt ttatcaataa tcagaacatc gcagtaaagc agctcaattt
      361 tttttgtttc accaggctgt ttcttttttt cctgtggtaa agaagtgttg aatgccgcaa
      421 atgtcagcgc ggcaaagaaa agaattatac cggttgtgta aatttttgaa ggttgccggt
      481 ggccgtttat ttcagccata gtggatttga aaaggggcat gatttgaatt cgtcacttat
      541 ttttatataa gtgacttcta tctttttatc aatccattcc agaaatcttt tctgtctctt
      601 ctcaagcaat gccgcattgt acagagcctg gtaatcttct ttcaggtcag caacatgagc
      661 tggaatctga ttgtcgagtc ttacaattct gaaaaccgtg tttccgttct cgtcctctgt
      721 catgaatggt tttgagattt cacctggctt cagatcccgg acaaccatat aggtgttcct
      781 gtccagctct tcaagggtaa acagtgtagt tctagctgca ggatcatttt tcacaagctt
      841 acccccattt atcttgctgt ctttgtgagt tgaaaacaga tatgctgctc tttcaaaagt
      901 tatactgtcg gctttgatta gattggctat actgtcgagt tttgaaagag ctctctcttc
      961 ctgctcaggt tttatttttg gtctgatcag aatgtgcctt gtattcacca gatctcccat
     1021 tctgtcgata agctgtataa tatggaaacc atacctggtt tcaacaattt ttgaaattgc
     1081 gttttttgta agtgagaaag caacatcggc ataaggcttt tcaagttcag cacggctaag
     1141 gtaaccaatc tcacctcctc ttttggccga ctctttgtct tcagaataga gtattgccat
     1201 tgtgctgaaa ctttttcccg caagaatttc acttctgata tcaagtagtt tctgtcgggc
     1261 ttcggccttg ttcaattcat tatcgggagg atcgagctga attatgctta attccacttt
     1321 ggcaggtatt gtcgggagac tgtcaggcgg aatcttggaa tagaattttt ttacttcagc
     1381 aggcgttaca gttatgtttt ctgcaatttt gctctgaact ctgttcacta tctcctgttc
     1441 tttgagagat tttctaagat ctgctctgat ttctgtaata cttttcctga aaacttcctc
     1501 aagagctttt tcagatcctg cccttgctat gaatttattc agcctttcat taagataacc
     1561 ctccacttca gagtcggaaa cttctatact gtctattctt gcctgatcca gaaacagctt
     1621 tgcaatcaac tgttcctcaa aaagattaca tctcagcctg tcagctggag ttctatcacc
     1681 cgaccataac tgctgtgcca cggcattttc gatgtcggaa aggaaaataa cttcgttgcc
     1741 cacaatccct gcaaccgatt caacaagctt ctgggaaaat aaggttcctg tattcaatac
     1801 caggaaaagc atcaacaggc ttataactgt tttatgtttc atttttcagt atttcttaaa
     1861 aatgttagaa ttaatagcat cattatatat gtcgttttcg agctttttca gaaactcgaa
     1921 tcttctgtta ttcaatataa tactttttat gtcgtcttta acatattcat atggtgccag
     1981 agaggacctt aatctgtagt cccttatggc aatgaaataa actgacgatg agtctctggt
     2041 ttcatagtaa ctgtatcgtt ttagaaactc atcaggattt attatttcag caggaagttc
     2101 cactgcaagt ttatcaaaag aaatccattc ttctccaaaa tcgtcaaatt tttccgcaaa
     2161 ctggaaacaa tatgattcaa gctgccttaa tgactccgag tcgtttgccc tgtaccatac
     2221 ccttaccctg tcaatatttg gggcttctgc aggtatcttt ataaaaagag ccttaactat
     2281 acttgtatta agtttgaaac ggtcgggatt tgaagcataa aattgctcta tttctgaatc
     2341 tgatacgatg gtgtccattt tttcaagcat catctgatgc tggtagtggt atatatacag
     2401 atttaccttt gtgtccagca actgcgattc aatatcattc ttatactccg gagagagatt
     2461 ttcaacagcc cttgcatata atagttccct tcttgcccag ctgttaatat aatcattgat
     2521 cgagactata ctatcttccg gggatttagt atttctgtaa atttcaggga tctggtcata
     2581 ataaagaatt ttttcacctg ctcttgcaac aggaattctt gccggacctt tattttgtct
     2641 caggcatgat atatctgaaa cagataaagt gataattgat aggtatatta aagcttttct
     2701 catttttgaa gttcattttt aacctgttcc agaatagttc tgtttattct aacggggtac
     2761 ttttccttta attgtttgat ccattctgac tcaagaaaat tctgatattc ggacgaaatt
     2821 tcacccatta cttcattcag tggcaggggt gaggcaggat taattttcgt aataactata
     2881 acagacggaa agccttttat ttcagtttca tgcagaccct cttcccattt aagattctca
     2941 ggcagaagag aatctccctc ctgaaacact cctttataca ctttaataat agtatcactt
     3001 cctgcattaa acattgcttg cagttttttt tcaaaatcag gtttctttac atattttctg
     3061 gctgccttat aaaattttcc ggcttttgct ttttccatgc acacataaat cataccggtg
     3121 aaactttttt taccggggta tctctgcttg ttctcttcgt aatatttttt taaaccagaa
     3181 gtatcgttct gggcccggtc ccagattttt tcctttgaga tttcaaacaa caatatgcca
     3241 tcatgaaatt cattgacgag gtatctgaat tcaggatatt ttttttcaag caatgaattt
     3301 tcatatctaa gaatatggtc tgttatgcaa gtttcagcca gcttgttaac aaaattcgca
     3361 ggttcttccg tgttattact tgaggctctg ctttctatat agcgggcgaa atcaacattc
     3421 gaaaactttt gaccgtcaaa tgtatacaac gttccagaag ggatagcacc cctgtcgtac
     3481 tttgaaagac ccctgatgat gagtgtatcg gtattttcta gaaaccagtt gagagcagtt
     3541 gtattgagcc ggaatttata ttcctgtttc aatgaattta ccattgagcg tgtagccagc
     3601 gagtttaacc atgtttcatc aattttactt tcaagctggc ttctggcttc ttcatatgaa
     3661 ggagggtctt ttttatcgag tctcttgatt atatgccagc cgtatattgt tttaacagga
     3721 ggagtgaagt ctccgttttt ttcgagcgaa aaagctgctt cggcaaattc gcttatgatt
     3781 tcgccagctc cgaaccagtt cagttcacct ccccgggctg ccgactcttt atggtcagaa
     3841 tatttcatcg caaggtcact gaaagaagcc ccttcgagaa gctttttata tatatatttt
     3901 atggtatcct cagcggcttt tgcaacttgt tcaggagcgt ttgggggcac actgcgcatt
     3961 atgtgagcca ctttaatttt tcccctcgaa ggtctgatgt cagccacttt gattatgtga
     4021 tacccataag gtgttcttac aggattggaa agttcccctt tcttcatgct gtaaacagcc
     4081 tcttcaaaag gtgtaatcat ctgaaaagta gtaatatatc caagattacc tccattgtat
     4141 attgctgaag ggtcatctga tgcgctgcgt gctacctgtt caaatggttc ccctgaaata
     4201 atccttgctc ttatttcaag agctttttta aatgcagcaa gtgtatcttc aggagaactg
     4261 ttaggcggac atgaaaccag aatatgccag gcatttattt ctttgagggt tttttcgtat
     4321 gattttttca gaatttcttc ttttacattt ttatcagtaa ggtaattctt cgccacctgg
     4381 tccctgtagc cctgtaattc tgtcctgaat gcttttgttg tgtcatatcc tttacttatg
     4441 gcatcagcga cttttaactt gaaaactata aactgttcaa gataattatt aaaatcaccg
     4501 gtatcggatg ggttatatat cttcctgtac atcctcataa attcccctgc tgtaacctct
     4561 cttcctccgg caattaaaag aatactgtcc tgtattccct gagcatgcag gagccccgtt
     4621 gcaatcacca taagaatgat ggagagcctg tatttcatta accggtctgg aaacttttat
     4681 tcaactgatt ttctgctgta gtttggagct tctcttgtga ttgttacatc gtgcggatgg
     4741 ctttcaatta tacccggtcc ggtaatcctt acaaactttg cttccttttt cagggtgggg
     4801 atatcggggg ttcccagata gcccatgcct gctcttaatc ctcccaccat ttgatacatg
     4861 acttctgcga gggttccttt ataaggtact cttgcttcaa taccttcggg tacaagcttc
     4921 tttatatcgt cttctacatc ctgaaagtag cggtcacgac ttccctgcga cattgcttca
     4981 agggaaccca ttccgcgata tgttttgaat tttcttccat tatatataat agtttcacca
     5041 ggagattcct caacgccggc aaaaagagac cctgccatta tggagtcggc acccgccgct
     5101 attgctttta ttatatcgcc cgaatacctt atcccaccat cagcaattac cggaacacct
     5161 gagccttcga ttgcttttgc cacattataa atggccgaaa gctggggaat tcccacgcct
     5221 gctataaccc tggttgtgca tatcgaccct ggaccgattc ctactttaac tgcatcagca
     5281 ccttccttaa ccagcctcct ggcagcctct tccgtgccaa tgttgccggc aactacgtca
     5341 acattcggaa aattcctttt aatatccctg agaattccga ttacattttt agtgtgacca
     5401 tgtgccgtat caataacaag agcatcgaca tttgcattta tcaatgcctc tgccctttcg
     5461 agagtgtcag ctgctatacc tattgctcct gccaccctga gcctgccctt ttcatccttg
     5521 catgcattgg gtctgtcttt cgatttggta atatctttat aggtcataag cccgattagc
     5581 ttgttgtttt catctactat gggcagcttt tctattttat gcttctgaag aattcttgcg
     5641 gcttcctcaa gatttgtctg ataggttgtg gtaatgagtt tggttgtcat tacctccgaa
     5701 atttttttag aagagttgtt ttcaaatctt aaatccctgt ttgttacaat accttttaat
     5761 atcccgtttt tgtcagttac aggaatgcct cctatcttgt attctcgcat caggcttatt
     5821 gcttcggcaa cagtagcatc gggatgaatt gtgacgggat cagttatcat tacattctca
     5881 gcccttttta cctttcttac ctgagaagcc tgtttttcta tagacatgtt tttatgaatc
     5941 acgccaatgc caccttccct tgcaatggca atggcaagct cagcttctgt aacagtatcc
     6001 atggctgcgg aaacaaccgg agtattaatg cgaatatttc tggaaaacat ggaacttata
     6061 tcaacatctc tcggcagcac ttctgaatat gcaggaataa gcagtacatc atcaaatgtt
     6121 aaaccttcaa acaaaatttt atctttaata aacgacatgg cttatgattt tggaatttac
     6181 ggtcgcaaat tacaaaaaaa aagcactcct gaaacattat ggaatatttt tgattaatat
     6241 aaaaaattga atcacagtta ttatttttgt atttgagggt actaatactt cagttggtaa
     6301 aaacattaaa gaaaactgtc tgaaatcatg gatatagaaa cctgcagaaa gatacttacc
     6361 agatactggg ggtttactac tttcaaacca ctgcaggaag agattataaa gtcggtactt
     6421 gagaagaaag acacactcgg acttatgccg accggaggag gtaaatcagt tactttccag
     6481 gttccaggta tagcactcga aggaatctgc cttgtaataa ccccactgat tgcccttatg
     6541 aaggaacagg ttgcaaggct caacaaaatg gagatcaaag ccctcgcatt acattcagga
     6601 atgacaaaag aagagattga aataactctt gacaactgta tatacggtga ttacaagttc
     6661 ctttatatat ctccagagag aatagcttca ccgcttttta gaattaaatc ccccaggctc
     6721 aatgtttcac ttgttgccat cgacgaggct cactgcatct cacaatgggg ttacgatttc
     6781 agaccgtctt acatgaaaat tgttgaactg agggaactac tccagaccga tccacctttt
     6841 cttgctctca cagcatcagc aactcctaag gttattgatg atataataga aaaactggct
     6901 ttcaaaacta gaaatgttct gaaaacgagt tttgaaagga aaaacatatc ttacattgta
     6961 cgaaaggttg aagataaaag ctcatatctt cttaatactc tgggaaaagt caaaggctcc
     7021 gggatcattt atgtaagaag cagaaaaaag gcgagggaag ttgctgagat gattgcatcg
     7081 aaaggttaca gcgccgatta ctaccacgct ggattgccac aggaactgag ggacaggaaa
     7141 caggctgcat ggtcgttgga ccaggtgagg ataatggttg caacaaatgc cttcggaatg
     7201 ggtattgata aacctgatgt aagattcgta attcactggg atataccgga atcaattgaa
     7261 tcgtattttc aggaaacagg tagagcggga cgcgacaata agccctcatg ggctgttctg
     7321 ctatattccg atgctgatat tagcaggctg aaggagagca tctcacataa atttcctcca
     7381 attgagaaaa taaaagacgt atatgaaatg ctttgcaatt acctccgtgt tccggtaggt
     7441 tcaggtaaag atacagtaca tgatttcaac atgcaggaat ttgtagccca gtaccacctg
     7501 cccgtgatag aaacatataa ctgcctggca tttcttcagc gtgaaggtta tatagaattt
     7561 accgaagaaa taaataatcc gtcgagagtg catttcatag taggacgcga cgacctttat
     7621 aagttccagg ttgccaatga gtcgtacgat aagtttatta aaatcctcct gaggtcttat
     7681 accggtatgt ttactgaata tgtacctgta aatgaagatg cactcgctaa aaaagcgggc
     7741 atgactcgtg aaactgtata tcagcttctg gtcagactct cttccatgaa tattctgcat
     7801 tatatacccg gtaaaaagac accgctggta atttttacag aagagagact cgacaggaaa
     7861 accctattta tttctcccga aaactacctg catgttaagg agaagtacct gctgagactg
     7921 gaaaaaatgc tggaatatgc tgaatcacag aatagatgca ggtcggtaat attgctggaa
     7981 tactttggag aggaagccga caggtgcggg atttgtgatg tatgcagaaa aaggaacgaa
     8041 ctagaattga gcaagtatga atttgatctg atacttgaag agattaaggc tgttctttca
     8101 gttgagaaac ctgatgccag ggaactggtc agaaaaataa attatccgga agaaaaagtt
     8161 ataaaagttg ttcaatggct gctcgaccat aataaaatta aacaggatac cgaaatgaaa
     8221 ctcttatgga atggctgata agttgtaata aaaataagtt tcccggatac atttataatt
     8281 ttctgtcctt aaaataggaa cacgatattt ttgcctaact ttgtcggttt gataaaaaga
     8341 ggagattgat gaacaaaaat caggagccct tctattccgg aacagttgtt gaatttgttg
     8401 catctgcaaa cgagtactgt aaatatatgg aacgcgctga agaacttgag ggtgaagagc
     8461 tcttgaagat tcttcaacga cttctgcctt ttatttacat caaaggttca ttgctgcctg
     8521 agcttgatcc atattttgaa gaaggaaatg agaaatttgt taaagaagaa gactggaaca
     8581 ggatatatga gacccttaaa caaaaattcg gaactgctga tgcgttcctt gaggttttcg
     8641 atgagaggat gaaagaatcg gaagcacctg ttgttgcgag ccttgctgaa aaccttgctg
     8701 atatatatca ggatcttaaa gattttttaa ttctttatca gacaggaacc aatgaaataa
     8761 tgaatgacgc tatatgggag tgcaggatga attttgaaaa ttactggggt cagaagctgg
     8821 taaattcatt gagagctata cataggcttg tttattcagg tgaagaaatc gggaaagtgc
     8881 ctgaggaaga gagtgaaaaa gagaaaatgg aaagactgga aaagtggata atcagccaga
     8941 aacaaaaaga attcagaaaa aaccggaatt aaagtgtatc ttaataatat tacacctgag
     9001 gaactttcga attacagggt tgggtatttt agcgggagga ttatcgtaat tgacagcatg
     9061 gaaagcttcc gccttcattc agattttctc accggcagga agatattggg ctttgatact
     9121 gaaacaaaac cttcattcag gaaggggaga agaaacaggg tttcacttct tcagctggca
     9181 tatgacaata ccgctttgct cttcaggcta aataagatcg gattgcccgg tgagcttgta
     9241 aaagtactat ccgatcccgg aataattaaa gttggcgttg ctattcatga tgatataaga
     9301 gtcctgcagg atttgaagca ttttaaccct gcaggatttg ttgatttgca gaaactggta
     9361 aaggagtatg gtatcgaaag tgcaggtttg aaaaagattg ctgcaattgt actgggtttc
     9421 agaatttcga aaagccagca ggtaaccgac tgggaggcac ctgtcctttc agaagcccag
     9481 aaagtctacg ctgctaccga tgcatgggtc tgttatgagg tgtacagaaa actgacaggg
     9541 caatgaaaat aaacatcacc tgaaatgatt gaaggcaagc aggaatatat aagaattgtt
     9601 cttaaatcag ggaaagatca atcggttcgt agatatcatc cctggatctt ttcaggtgct
     9661 attaagaaga tttacggaac acctgccgag ggtgatattg tggaagtata cgacaacaag
     9721 ggagagttcc tggcaacagg ccattatcag cctggttcaa tagctgtcag gatcctctca
     9781 tttgaaaagg taatacctga ccgggatttt ttcaggaaga tgatttcttc agccgttgaa
     9841 tacaggaaac aacttggctt tttatccgat cccagcacaa atgttttccg tctgattcat
     9901 gcagaaggtg ataacctgcc aggactgata gccgattatt ataatggtgt ggtcgtactg
     9961 cagatgcatt caaccggaat gtacaggata agaaatgaaa tagcagaaat tatttcggaa
    10021 attctttctg aacatgtaca ggctgtttac gacaaaagtg aagcgacaat ttcgtttaag
    10081 tcagatatta ggccagaaaa cggattcata tcaggcagtg cagaaccttc aataatactt
    10141 gaaaatggtt ataaatttta cgtcgactgg attactggtc agaaaacagg ttttttcatt
    10201 gaccagaggg aaaacaggaa gctggtacag cattattcat gtaatcgtga tgtcctgaat
    10261 ctgttcgggt acactggcag cttttcagta tatgccatga aaaatgcccg tagtgttgat
    10321 acagttgaca gttccggtcc ggcattaaag attgccgatg aaaacatacg tttgaattac
    10381 ggtgaggatt caaggcatca ctcttatgaa gctgacgcat ttgagtttct tgctggttgc
    10441 cgcaacaggt atgatttgat gataattgat cctcctgctt ttgcaaaaca taatgatgtc
    10501 cttgcgaatg cactacaggg ttataaaagg ctgaatatta aagcaattga gaatataagg
    10561 ccgggtggaa taatttttac tttttcatgt tctcaggtgg tttcaaaaga gaatttcaga
    10621 aaatctgtat ttgctgctgc tgcaaatacg gggagaaaag taagaattct gcatcaactt
    10681 ggtcaaccgg ctgatcatcc ggtaaatata tatcatcctg aaagtgaata tcttaaaggg
    10741 ttggtacttt atgttgaatg aaggtagaga gtaagtattg ttactttttt tcatgtgtga
    10801 gattttatta tatgtttgat gttggataaa atgcctgata tgagtgttga caagcatttt
    10861 gaatttattg caaacaagct cgggctgaag gtatggcagg tcgaaaatac gattcggctg
    10921 atggacgacg gagcaactat acctttcatc agcaggtaca gaaaagaatc gacaggtagc
    10981 ctcgacgagg tgactctcct tactatcaag gatgaatatg cccgtcttaa ggaactcgat
    11041 aaacggcgtg aagccataat acgttcaata gaggaacagg gcaagatgac accggaatta
    11101 cggcagagga tcgatgatgc tttaactctg acagaactgg aggatattta cctgccttac
    11161 cggcctaaac gcagaacccg cgccacaata gcccgtgaaa aaggccttga gccacttgca
    11221 cgtttgattt atgagcagac tgaaaaggat cccgtcgcaa aagctgcaga atttcttaac
    11281 gaacaggtgg ctaatgctga tgaagcaat
//
LOCUS       DAOSNB010000043        11254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5754_length_11254_cov_4.248951, whole genome shotgun sequence.
ACCESSION   DAOSNB010000043 DAOSNB010000000
VERSION     DAOSNB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5754_length_11254_cov_4.248951"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLG42_03230"
     CDS             complement(<1..254)
                     /locus_tag="PLG42_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU30258.1"
                     /translation="MKKSITSKEEASEKKFDLEERLIDYAVDVIRLVESLPDTKTANH
                     LGGQLLRSGTSPSLNYGEARGAESKNDFVHKMKVCLKELR"
     gene            complement(442..1836)
                     /locus_tag="PLG42_03235"
     CDS             complement(442..1836)
                     /locus_tag="PLG42_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOU30259.1"
                     /translation="MKKTILTLTIILLIGQIMGQQPAAQKVLTKDERMAWWREARFGM
                     FIHWGVYAIPAGIWNGREIGGIGEWIMNRGKIPVRDYMEMAKQFNPVKYDPDAWVQMA
                     KDAGMKYIIITAKHHDGFALFKSEASKWNVVDHTIYGKDLLKPLAEACQRHGIKLGFY
                     YSQAQDWVNPGGSAARKLTKEGWPNPDSVIIDAYTKEHNGHWDPAQETRTFAQYIDEV
                     AVPQVRELLTNYGKVAVLWWDTPTGMTDEAATKLQALLALQPEIITNDRLKRPNFPGD
                     HKTPEQRIPNLADLDGTDWETCMTMNNTWGYKSYDHNYKSPQTLIRNLIDIASKGGNF
                     LLNVGPTAEGEFPPESVEILREIGKWMKINGEAIYGTTASPWGLFDWGRCTKKTTKNG
                     TTFYFSVFNWPQNGKLTIDNFTGKVTSAKLLATGAAVKTSLTGKTLTINLPPSAPDPI
                     ATVIKVEARGGKNK"
     gene            complement(1861..3378)
                     /locus_tag="PLG42_03240"
     CDS             complement(1861..3378)
                     /locus_tag="PLG42_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOU30260.1"
                     /translation="MSRNQMTNLTQQKAVKLPVYNITDFGARGDGKTLDTPSINKAIE
                     EAAARGGGIIWFPAGNYLSVSIRLKSNISLYLDQGAVIIAASTSDGYKYDPPEPNIWG
                     DSLQYQDFGHSHWHNSLIWGENLENISILGPGLIWGKGLVRGTSNAPQGSGNKAIALK
                     NCRNVILRDFSILMGGWFGILATGVDNFTIDNLKIDTNRDGIDIDCCINVRISNCSVN
                     SPRDDGICLKSSYALGEPRPTENITITNCQLSGFTEGSFLDGTYKRDEKTAGGNGPTG
                     RIKFGTESNGGFKNITISNCIFTFCRGLALETVDGALLEDVTITNITMRDIVNAPIFL
                     RLGERMRGPAGTPPGELRRVIISNITVYNADLKNGCIISGDKGHEIKDVILSNIRIYY
                     KGGGTAEQALREVPGFEKEYPEPYRFGIMPSYGFFIRHVDGIQLENVEVRYMEKEFRP
                     AFYLDDVRNAEFLFVKAQKEEGIHSIALRNVSNLILFRSLGFDDRKIEKIENGKF"
     gene            complement(3530..4525)
                     /locus_tag="PLG42_03245"
     CDS             complement(3530..4525)
                     /locus_tag="PLG42_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU30261.1"
                     /translation="MKIKNLILFVLAIMVSANLPGQKTPTGNPADFRVKTCLHSVSYM
                     GVWRGQATLTVDQFLVKAKELGFDGVMLAAKRPHVSPLDYDDAARARLKARIKELGLE
                     LVCIAGYCDFTAGVDKAGIPNVEIQALYVGELARLARDLGTNMVRIYTGYERPDIPYD
                     KQYAMVVEGLQMAGKIAAKYGVTLAVQNHHDIALHPDAMKWLLDEVNLPNVKAAFDCW
                     SPTLAGLSPEEIKQAIYKLKPYIVHTTTADYVSLPRVIYDLEHTNYINKTPQMRAVPI
                     GKGFLDYKTFINTLKEIGYQGYIAYEMCEVLEGGGSIENLDKTAKAFLEYVKQFR"
     gene            5065..8304
                     /locus_tag="PLG42_03250"
     CDS             5065..8304
                     /locus_tag="PLG42_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU30262.1"
                     /translation="MIRLFAFFVICAVTVVAAGNLNASAGMYTGDDYVLAGGGEQQQF
                     TVSGTIRDASTGEPMAGVTILVKGTTTGRLSDESGRFTIPLPGREAVLQFSFIGYSTQ
                     EISVTAGSVINVNMTTEEQKIEEIVVVGYGVQKKESVVGAITQVNSQSLMQAGAATVT
                     NAIAGKLSGVLTIQQRGEPGNNAAEIYIRGVSSWNGSAPLVLVDGVERDWSSLDPNEI
                     NTISVLKDASATAVFGAKGANGVIVVTTKRGALGKPKMDLTASYGLLKATRIPDHIDS
                     YTTMSMYNVALMNEGGTYFSQLIPDWQLQEYKNPSTPLNALRFPNVNWFDICTQPFAP
                     TSVENLNVSGGTEFAKYFLNLGHQYEGSFFNGIKEGYQDSRFWNHLFNYRINLDFSVT
                     KTTTISLNAGGSLNLKNTPSSASWRNLYSTGPSRFPAYFPAWVLEMVPDPDYPEDKGM
                     RRAQAFGEYTGNPYTSLSQGSFNRDLGSRMFTDLIFKQQLDWLVKGLSANAKVSLSTY
                     YNNRQLTLSYTYPEYQLYYDLIPQSGQFNPATGRFINPWYRNGEGNEYWKLGPYDINV
                     GGLNGGYYRDLYYEASLNYSNTFGKHTVTALGLFNRQEKNQGTDFPYYNEGWVGRVTY
                     DYNRKYLFEFNIGYTGSERFAPANRFGTFPSLAVGWVASEENFFKAAVPWMNKLKFRY
                     SDGYVGSDYARSRWLYTSSYYKDGRGYIREDLGANMRAQWERAHKRDLGVEIAMFKNL
                     ISLNVDLFDEYRDKMLLTPKSVTMMVGNSFKELNLGKVKKHGIEIEAEYRKTVSPRFT
                     YFFRGIFSFNENRVIFKDDEPYAPEHRKQAGKPLTAMMFAEAGVEGTEINGVELTGTG
                     YYTTIDDIHNNVSPISLIQLRVGDYKFLDFDADGTITAAGDKYPIKGLTYPPIVYSFG
                     SGFTWGRFDFNILFSGNQGKYVQYNQLYEVEFIKGDWRVHKSQLDYWTPTNQDANHAN
                     LHYAGSSSSPILFWGGGEADRGYQIMIEDRFFRNASYLRLKDVYLSYTFKPGSLKNTL
                     GISNLMVYVTASNLFTITSLPEGDPEATNFQQGFYPIIASGRLGVKINF"
     gene            8324..10177
                     /locus_tag="PLG42_03255"
     CDS             8324..10177
                     /locus_tag="PLG42_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU30263.1"
                     /translation="MKKYIYYTAFFCMSLLLFVSCEEYLDKAPEEGIKEEEIFSKIVN
                     FKLFFNAVYEGQSIYNNNWRDFNIKTGHSLYFDYWDQKYTIEGITDAADQGRYMEGQV
                     FKQGSVIGFVDKFINDGNRRPILKSMFMDIRICNIALRKLDMLQDATQQDKDDLRGQA
                     HFIRAYCHFTLFKWWGRMPYITHVIGPEDPWDIPRLSNYETCIQIAKDLDSAYYFFEK
                     AGKVRRDPGPGLPGHLNDPDQRAPNGVAAKALKSRVLLYAASPLNNSVGQSAWEEAAK
                     ASWEALQLALSNSYALMPGNERYKNFYGNNYCNEQLWAWAAGSLSWNSGNFAGLYNGV
                     FGASKSSWSGVCPTQNFVDKYETSFGEALNTDADRAAAIAAGHYNEQNPYANRDPRLA
                     MDIITNQSPCVGWSSGKAQIWRQTSGGTTTYSELLDQSYLGITRTGYYLRKNWGNNSQ
                     KNKVNVVHTDPIIRLAELYLNYAEASNEAWGPTTIGVPGATMNAVDAINVIRQRVGQV
                     NVLPQYTVSKEVFRDRIKNERNVELSWEGHYYFDIRRWMDAPQCYSTTLMGVDIEKLP
                     APTAEYPTGYKYTRVPLGQDRQVAWKPAMYYLPFNIVDNFKMRIFEPNEVW"
     gene            10200..>11254
                     /locus_tag="PLG42_03260"
     CDS             10200..>11254
                     /locus_tag="PLG42_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU30264.1"
                     /translation="MRLNNIFHRLRIASLILVCAMLFTEVYGQAQRQQRRARQLIDVT
                     IRVIDDEGNPVPGASVVVGEGVIHTETDANGTVSFKAYPDNFITVTSPAFEKSVSLVT
                     DLIVNNTITLVRSKLYMTRADEVPLPFTSLKKRQLTGPETTVLASRLEKYPTNDIRAT
                     FTGLTSGWDVRELYGWPGVTEREPIGVFGATNKFTNVPIAIVDGIYTDLSEMPIDASE
                     IESITVSKGTLAAAMFGPVTAKNGFLFIKTKRGYVNERTLDFNIERGVSIIDRMPEWV
                     DGVDYAKLNNQARENDGLTPLYSTEAINNFAKNDPYDLRYPNVNYRDMILKNNMPFMR
                     ANMSASGGNDVVQYYSY"
BASE COUNT         3104 a         2363 c         2496 g         3291 t
ORIGIN      
        1 tctctcagct ctttgagaca aactttcatt ttatgaacga agtcattctt tgattcggcc
       61 cctcttgctt ctccataatt aagtgaaggt gaagttcccg acctgagcaa ctgtcctcca
      121 agatgattcg cagtttttgt atccggtaaa ctttccacaa gccttataac atccaccgca
      181 tagtcaatca gcctttcttc tagatcaaat tttttttcag atgcttcttc tttagatgta
      241 atagactttt tcatttttta attatgtgta ttacaaatag ttctcttttt caattgttcc
      301 cccgttcaat tgttcaattg ttctgttgtt caattgttct attgttctct tgttcatttg
      361 ttcaattgtt caattgttca attgttcatt tgttcaattg ttcaattgtt caattgttca
      421 tttgttcatt tgttcatttg ttcatttgtt cttccctcct ctcgcctcca ctttgatcac
      481 cgtagctatc ggatcaggtg ctgagggtgg aaggtttata gtaagagttt ttccggtgag
      541 ggatgttttg accgcagctc ctgttgccag cagtttggct gaggtaactt tcccggtaaa
      601 attgtctata gtcagttttc cgttttgtgg ccagttaaag actgaaaagt agaaggttgt
      661 tccgtttttt gttgtctttt ttgtgcacct gccccaatcg aatagacccc acgggctggc
      721 agttgttcca tatattgcct cgccattgat tttcatccat ttgcctattt ccctgagtat
      781 ttcgacgctt tccgggggga attcaccttc ggcggtaggg cctacattaa gaagaaaatt
      841 gcctcctttt gatgctatgt cgataaggtt tctgattagt gtctggggag atttataatt
      901 atggtcgtag cttttgtatc cccaggtatt gttcatggtc atgcaggttt cccagtcggt
      961 accgtcgaga tcggcgaggt ttgggatgcg ttgttcgggg gttttgtggt cgcccggaaa
     1021 attgggtcgt ttaagacggt cgtttgtgat aatttcgggc tggagtgcca gcaatgcctg
     1081 caattttgtt gcggcctcat cggtcatgcc cgttggtgtg tcccaccaga gaactgcaac
     1141 ttttccataa ttggttagta gctctcttac ctgtggcacg gccacttcgt cgatgtactg
     1201 agcgaaggtt ctggtctcct gtgcaggatc ccagtggccg ttatgttctt ttgtgtatgc
     1261 atctattatt accgagtcgg ggttaggcca gccctctttt gtaagtttgc gtgcagctga
     1321 gccgccgggg ttgacccagt cctgagcctg cgaatagtaa aagccgagct ttataccgtg
     1381 ccgctggcat gcctcagcaa gtggtttgag aaggtctttg ccgtagatgg tgtggtcaac
     1441 tacattccat ttgctggctt ccgacttaaa gagtgcaaag ccatcgtgat gctttgctgt
     1501 aatgattata tatttcatac cggcatcttt tgccatctgg acccatgcat cggggtcata
     1561 ctttacagga ttgaactgtt tggccatctc catataatct cttaccggaa tctttcccct
     1621 gttcattatc cattcgccta ttccgcctat ttcacggccg ttccagatgc cggcagggat
     1681 ggcatataca ccccaatgaa tgaacatgcc aaagcgcgcc tcgcgccacc atgccatacg
     1741 ttcgtctttt gtcaggactt tctgcgcagc aggttgctga cccataattt gtccaattaa
     1801 taaaataatt gttagtgtta aaattgtttt tttcattttc ttttatttta agtagatagg
     1861 ttaaaatttg ccattttcaa ttttttcaat cttcctatcg tcaaaaccga gtgagcgaaa
     1921 caaaatcaga tttgagacat ttctgagtgc aatagagtga ataccttctt ctttctgggc
     1981 tttaacgaaa aggaattcag catttctgac atcatcgagg taaaatgctg gcctgaattc
     2041 tttttccata tatctgactt caacattctc aagttgtatg ccatcaacat gcctgatgaa
     2101 gaagccgtaa gaaggcataa ttccaaaccg gtaaggttcc ggatattcct tttcgaaacc
     2161 gggaacttct ctcagtgcct gttcagctgt tcctcctcct ttataatata tcctgatgtt
     2221 tgaaagtatt acatctttta tttcatgtcc tttgtcgccg cttataatac aaccgttttt
     2281 caggtcggca ttatagacag tgatattgga aattattact cgcctgagtt ctcctggtgg
     2341 agttcctgcc ggtccccgca ttctctctcc aagccttaga aatattggtg cattcactat
     2401 gtcgcgcatt gtaatatttg ttattgtgac gtcttccaga agtgcaccat caactgtctc
     2461 aagtgcaagt ccccggcaaa aagtaaaaat gcaattggag attgtaatgt ttttgaatcc
     2521 tccgttcgat tctgttccga attttatacg gccggtcggc ccgttccctc ctgcagtttt
     2581 ttcatctctt ttatatgtgc catcgagaaa agatccttca gtaaatcctg acagttggca
     2641 atttgtaatg gttatatttt cggttggcct cggctcaccg agtgcataac tgcttttaag
     2701 gcagatcccg tcatcacggg gcgaatttac gctgcagttt gatattctta catttataca
     2761 acaatcgata tcaataccat cgcggttggt gtcgatttta agattatcta tagtgaagtt
     2821 gtcaaccccg gtggcaagta taccaaacca tcctcccata agaattgaaa aatcacggag
     2881 aattacattg cggcaatttt tgagggcaat agccttgttt ccacttccct gaggagcgtt
     2941 gctggttcct ctaaccagtc ctttacccca tatcagtcct ggcccgagta ttgaaatgtt
     3001 ttcaagattc tcaccccata tcaggctgtt atgccagtga ctatgtccga agtcctggta
     3061 ctgaagtgag tcaccccata tatttggctc tggcgggtca tacttataac catcagatgt
     3121 tgaggcagct attattacag caccctggtc gaggtaaagg cttatgttgc tctttagcct
     3181 gatagagaca gaaagataat ttccagccgg gaaccagata ataccacctc ctctggctgc
     3241 tgcttcctct atagctttat ttattgatgg tgtgtcgagc gtttttccgt ctcccctggc
     3301 tccaaagtca gtaatattat aaacaggtaa tttcacggct ttttgttgcg tcaggttagt
     3361 catctgattt ctggacatgc cagctgatgt caatgaactg acatagaaaa acacaccgcc
     3421 agagcagaga attgcaataa taaatttgta actcagtgaa aaatatttat ttaatctgta
     3481 cataattaat ttccaactta ttaatgtaat gacttaatgc ctgctgctat tacctaaact
     3541 gtttaacgta ctctagaaaa gcctttgcgg ttttatccag gttttctatt gaacctccgc
     3601 cttcgagtac ttcgcacatc tcataggcaa tatatccctg atagcctatt tccttaagtg
     3661 tgttgatgaa tgtcttatag tcgagaaatc cttttcctat tggtacggct ctcatctgtg
     3721 gggtcttatt aatataattg gtatgctcca gatcgtagat tacccttggc aagctgacat
     3781 aatcggctgt agtagtgtgg actatataag gtttgagttt atagattgcc tgtttgattt
     3841 cttccgggga gagaccggca agtgtcggtg accagcagtc gaatgcagct tttacgttgg
     3901 gcaggtttac ctcgtcaaga agccatttca ttgcatccgg atggagtgct atgtcgtgat
     3961 ggttctgcac ggcaagggtg acaccgtatt ttgcggctat ttttccggcc atctgaagtc
     4021 cttccaccac cattgcatat tgtttgtcgt aggggatgtc gggtctttca tagccggtat
     4081 agattcgtac catatttgta cccaggtcgc gggcaagacg ggcaagctca cctacataga
     4141 gtgcctgtat ttcaacattg ggaatacctg ccttatctac tcctgctgta aaatcgcaat
     4201 agcctgctat acaaaccagt tccagaccga gctctttaat tcttgcttta agccgggctc
     4261 tggcggcatc gtcataatca agtggtgaca cgtgggggcg ctttgcagca agcatcacac
     4321 catcaaaacc aagttcttta gcctttacaa ggaactgatc taccgtgagt gttgcctgac
     4381 cgcgccatac acccatgtag ctaaccgagt gaagacatgt ttttaccctg aaatctgcag
     4441 gattgccggt tggcgttttc tgtcccggta aattggctga aaccataatt gccagaacga
     4501 atagaataag attttttatt ttcatacttt ttattattaa ttggcagata aaaatatgag
     4561 tttatttgaa atgttaaagt gttaaaaaaa ttaaaaaatt tttttgtact taaaaatttt
     4621 ttgtttaaaa ttgcaaagac tgaaagttat ttttaaatct gttgcacggc tagtaatatg
     4681 tctctgcagc agataagaga gcttaagttg ataaccctaa cctgacctat gagaccgaat
     4741 ctcagaaaag agataaaggt tttgagaaat tgtgaagatt gcaggcagtt taatgccagc
     4801 attctttttt tattttgtaa ttcgttaacg tacacgacct ttgcatcaga aatgaggcaa
     4861 aggttttgtt tttcagaaag atagaataat aaattttaaa aaccagagcg tatgacatga
     4921 gcaattaaaa atttaaaacc ggacagagga atagtaaaga attcctattc tgaggcaaaa
     4981 agtgattaat atttaatcag ggaataagaa atctcaaaac tttacgaaaa gagttaaaaa
     5041 gaatatccga taaaactaac gcttatgatt agattgtttg ctttttttgt tatctgtgca
     5101 gtgactgtgg tcgcggcagg taatttgaat gcatcagccg ggatgtatac aggtgatgat
     5161 tatgtcctgg ctggtggcgg tgaacagcaa cagtttaccg tatcgggaac aataagggat
     5221 gcctccacgg gtgaaccaat ggcaggtgtt accattcttg taaaaggaac aacaaccggc
     5281 cgtctatcgg acgagagcgg aaggtttacg attcctttgc ccggcaggga ggctgttctg
     5341 cagttttctt tcattggtta ctcaacccag gaaataagtg tgactgcagg ctcggtaata
     5401 aatgtcaaca tgactaccga agagcagaag attgaagaaa tcgtggttgt tggttatggc
     5461 gtccagaaaa aggagagtgt tgtgggtgct ataacacagg ttaacagcca gtcgctcatg
     5521 caggcaggcg cagctacagt aaccaatgcc attgcaggaa aactttccgg tgtgcttaca
     5581 atacagcaga gaggtgagcc gggaaacaat gctgcagaaa tatatatccg aggtgtgtcg
     5641 agttggaacg gctcagcccc tctggtgctg gttgacggcg tggagcgcga ctggtcgagt
     5701 ctcgatccga acgaaataaa cactatttcc gttcttaaag acgcttcagc aactgcagtg
     5761 ttcggtgcaa aaggagccaa cggggttata gttgtaacaa caaaacgagg cgcactcggt
     5821 aaacccaaaa tggaccttac cgcctcatat ggtctgctga aagcaaccag aatacctgac
     5881 catattgatt cctatactac tatgtcgatg tataacgttg ctctgatgaa tgaaggcggt
     5941 acttacttct ctcagcttat acccgactgg cagttgcagg aatataagaa tccttccacc
     6001 ccgcttaatg cattgagatt cccgaatgtt aactggttcg atatttgtac acagccattt
     6061 gcacctacat ctgtcgaaaa cctcaatgtt tcaggtggta ctgaatttgc aaagtacttc
     6121 ctcaatctgg ggcaccagta tgaaggctca ttttttaatg gtataaagga aggatatcag
     6181 gactcgagat tctggaacca tctgttcaat tacagaatca acctcgattt cagtgtgacg
     6241 aaaacaacaa ccatttcatt gaatgccggg ggatcactta acctgaagaa cacaccttcg
     6301 agtgcttcgt ggcgtaacct ttactccacc ggtccttcaa gattcccggc ttacttcccc
     6361 gcctgggtgc tcgaaatggt tcctgaccca gattaccccg aagataaagg aatgagaaga
     6421 gcacaggctt tcggtgaata taccggtaac ccgtacacta gcctctcaca gggtagcttc
     6481 aacagagacc ttggctcgcg tatgttcacc gaccttatct tcaaacaaca gctcgactgg
     6541 ctggtgaaag gactttcagc aaatgcaaaa gtttcactga gtacctatta taataataga
     6601 cagcttactc tttcatatac ttatccggaa tatcaacttt attatgacct catcccgcaa
     6661 tcaggacagt ttaatcctgc taccggacga ttcattaatc cctggtacag gaatggtgaa
     6721 ggaaatgaat actggaagct gggtccgtat gacataaacg ttggcggact taacggtggc
     6781 tattacaggg atttatacta tgaggcgtca ttgaattatt caaatacttt cggtaaacac
     6841 actgtaactg cactcggtct tttcaacagg caggaaaaaa atcagggaac cgatttccct
     6901 tattataatg aaggttgggt tggcagggtt acttatgact ataacagaaa atacctgttt
     6961 gagtttaata taggatatac cggttccgag agatttgctc ctgcaaacag gttcggtact
     7021 ttcccctctt tagctgttgg atgggttgct tcagaagaaa acttcttcaa agcagcagtt
     7081 ccatggatga acaagcttaa attccgctac tccgatggat atgtgggaag tgattatgcc
     7141 agaagcagat ggctttatac aagttcatac tataaagatg gtcgcgggta tatcagggaa
     7201 gatcttggcg ccaacatgcg agcacaatgg gaacgtgccc acaagagaga ccttggtgtt
     7261 gaaatcgcaa tgttcaagaa tctgatttct cttaatgttg atttattcga tgaataccgc
     7321 gataagatgc ttcttactcc caagagtgtt actatgatgg ttggcaacag cttcaaggaa
     7381 cttaaccttg gtaaggttaa aaagcatggt atagagattg aagcagaata cagaaaaacc
     7441 gtctcaccga ggtttacata ctttttcaga ggtatattct cgttcaatga aaacagggtg
     7501 atattcaagg atgacgaacc ttatgcaccc gaacacagaa aacaggcagg caagcctctc
     7561 acagccatga tgtttgccga agcaggtgtt gaaggaactg agataaacgg cgttgaactt
     7621 accggaacgg gatactatac cacaatagat gacattcaca acaacgtatc acctatttcc
     7681 ctcattcagc tgagagtagg agactacaag ttcctcgact ttgatgctga cggaacaatt
     7741 actgctgcag gtgataagta tccgataaaa ggtcttacat atccgccaat agtttattct
     7801 tttggctcag gatttacatg gggccgtttc gatttcaaca tcttgttctc ggggaaccag
     7861 ggcaaatatg tgcagtacaa ccagctgtat gaagttgagt ttatcaaggg tgactggaga
     7921 gttcacaaat cgcagcttga ttactggact ccaaccaatc aggacgccaa ccatgcaaat
     7981 ctgcactatg caggaagttc aagttcgccg atactcttct ggggaggtgg tgaagccgac
     8041 aggggttatc agatcatgat tgaagaccgc ttcttcagaa atgctagcta cctgcgtctg
     8101 aaagatgtct atctgagcta taccttcaaa cccggatcac ttaagaatac cctcggtata
     8161 tcgaacctga tggtgtatgt gacggcaagc aacctgttta ctattacatc actgcctgaa
     8221 ggtgatccgg aagcaaccaa cttccagcag ggtttctacc ccatcattgc atcaggaaga
     8281 cttggcgtaa agattaattt ctaactttta agggaggtca aatatgaaaa agtatatata
     8341 ctatacagca tttttctgca tgagcctgct attgttcgtc tcatgcgagg aatatcttga
     8401 caaagcccct gaagaaggta ttaaggagga ggagatattt tcgaagattg taaactttaa
     8461 acttttcttc aacgcagttt atgaggggca gtcaatttac aacaacaact ggcgcgattt
     8521 caatatcaaa acaggacatt ctctctactt cgattactgg gatcagaaat acaccatcga
     8581 aggaataacc gatgctgctg accagggccg ctatatggaa ggccaggttt tcaagcaggg
     8641 ttcggttatc ggtttcgtcg acaaatttat taacgacggt aaccgcaggc caatacttaa
     8701 atccatgttt atggatatca ggatctgtaa tattgccctg agaaaactcg atatgttgca
     8761 ggatgccacg cagcaggata aagatgacct caggggtcag gcacatttca taagggccta
     8821 ctgccatttt acattattca aatggtgggg caggatgcca tatattactc atgtaatagg
     8881 tcctgaagac ccatgggata tcccgaggct ttccaactat gagacctgca ttcagattgc
     8941 aaaagatctc gattcggcat actatttctt tgaaaaagcg ggcaaggtac gccgtgatcc
     9001 cggtccgggt ctgccaggtc acctgaacga tcccgaccag agagcaccta atggtgttgc
     9061 cgcaaaggca cttaaatcgc gtgtattact ttatgccgcc agtccactga acaattcagt
     9121 tggccagagt gcatgggaag aagcagccaa agcatcgtgg gaagcattgc agctggctct
     9181 aagtaacagt tatgcgctga tgcccggcaa cgaaagatat aagaatttct atggtaacaa
     9241 ttattgtaat gagcaactct gggcatgggc agcaggatca ttatcatgga acagtggtaa
     9301 ttttgcgggg ctttataatg gagtttttgg agccagtaaa agctcatggt caggtgtttg
     9361 cccgactcag aactttgttg ataaatatga gaccagtttc ggggaggctc ttaatacaga
     9421 tgccgacaga gccgctgcaa tcgctgcagg gcattataat gagcagaatc catatgcaaa
     9481 cagagatcct aggcttgcaa tggatatcat tacaaatcaa tctccttgtg taggatggtc
     9541 ttcaggaaaa gcacagatat ggagacagac ctcaggaggt acgacaactt actcggaact
     9601 cctcgatcag tcttatctcg gcattaccag aacggggtat tacctgagaa agaattgggg
     9661 aaataatagt cagaaaaata aagtaaatgt tgtacatacc gatcctatta tacgtctggc
     9721 agaactctat ttaaattatg ctgaagctag caacgaagcc tggggtccga ccacaatagg
     9781 agttccgggt gcaacaatga atgctgttga tgcaataaat gttatacggc agagggttgg
     9841 gcaggtaaat gttcttccac aatatactgt ttcaaaagaa gttttcagag acaggatcaa
     9901 gaatgaaaga aacgttgaac tgagctggga aggacactat tactttgata tccgccgctg
     9961 gatggatgca ccgcaatgtt actcaaccac tcttatgggt gtagatattg aaaagttgcc
    10021 tgcacccact gcagagtacc ccacaggtta taaatataca agagtgcctc ttgggcagga
    10081 tcgccaggtg gcatggaaac cggcaatgta ctacctgccg tttaacatag tcgacaattt
    10141 caagatgaga atcttcgaac cgaatgaagt ctggtaataa ctattaaata tttaagaata
    10201 tgagattaaa taatatattt cacagactca gaatagccag cctgattctt gtatgcgcca
    10261 tgttatttac agaggtgtat ggtcaggctc agaggcagca aagaagggct agacagctga
    10321 tagatgtgac aataagggtt attgatgatg aagggaatcc cgtacctgga gcctccgttg
    10381 tagtaggtga aggagtaatc catacagaaa cggatgccaa tggtacagtc agctttaaag
    10441 cctaccctga taatttcata actgttactt ctcctgcttt tgagaagagc gtatcacttg
    10501 tcacggacct tattgtgaac aacacaataa cactggtacg ttcgaaactc tatatgacaa
    10561 gagcagatga agtaccgctt ccatttacat cacttaagaa gcgccagctt acaggtccgg
    10621 agacaacggt cctggcttca aggcttgaaa aatatcctac caacgatata agggccactt
    10681 ttacaggact gacatctgga tgggacgtta gagaacttta cggctggcct ggtgttacag
    10741 aacgtgagcc aataggagtt tttggagcaa caaataagtt caccaatgtc ccgattgcaa
    10801 ttgtggacgg catatatacc gacctgtcag agatgcctat cgatgcatcc gaaattgaat
    10861 ctattactgt cagcaagggc acactcgccg ctgcgatgtt cggcccggtt acggcaaaaa
    10921 atggattcct gtttatcaag accaaacgtg gttatgtgaa tgagaggaca ctcgatttca
    10981 atatagaaag aggtgtaagc attatcgaca ggatgcctga atgggtagat ggtgtagatt
    11041 acgcaaaact gaacaaccag gcaagagaaa atgacggatt gactcctctt tattcaacag
    11101 aggctatcaa taactttgcc aaaaacgacc cttacgacct gagatacccg aatgtgaatt
    11161 accgtgacat gattcttaag aacaacatgc ctttcatgag ggcaaacatg tctgcttcgg
    11221 gaggaaatga tgtggtccag tattattcat atat
//
LOCUS       DAOSNB010000044        11012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5919_length_11012_cov_4.145569, whole genome shotgun sequence.
ACCESSION   DAOSNB010000044 DAOSNB010000000
VERSION     DAOSNB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5919_length_11012_cov_4.145569"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1319
                     /locus_tag="PLG42_03265"
                     /pseudo
     CDS             126..1319
                     /locus_tag="PLG42_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            1724..5947
                     /gene="glgP"
                     /locus_tag="PLG42_03270"
     CDS             1724..5947
                     /gene="glgP"
                     /locus_tag="PLG42_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOU30265.1"
                     /translation="MKVGFLFETSWEVCNKVGGIHTVISTKALNIVNELGDNYILIGP
                     DVWREDSTNPEFIPDDSMFIDWKNRAISEGLKVRTGRWNIAGRPIVFLLDFTPFFGQQ
                     NEIFARFWETYKLDSITGQWDYVEPALFGYAAGKVIESFTNFYHEHHDIVAQFHEWMT
                     GTGVLYLKKHAPWVATAFTTHATVLGRCIAGNNRPLYGKMTDYNPVNVAREFNVIAKQ
                     SLEKITAAEADVFTTVSELTSRECAHFLGKKVDLITPNGFENSFVPGPDVFNEKRQKA
                     REMLFRVAQAVLGYTPSPDAMLVATSGRYEFRNKGIDIFIDSLGEINRKAEIQKECIG
                     FILVPAYHKGPRQDIIDFLYNNGPVPSGDRYLTHSLHYPEPDAILNRIKSNGLTNDQK
                     NRVKVIFVPSYLNGNDGIFNMSYYDLLIGFDLTAFPSYYEPWGYTPLESMMFRIPTLT
                     TTLSGFGLWVKENFSDTGKSIRIINRTDENEPEVISSICSFISEFATLGEQEIAEARN
                     KAYEISKTLMWDSFVKHYFTAYDRALTVSFERRNEPREFIKMVEAPVLEIKKPHQVPV
                     WKDIYVQSDVPEKLAALKELSTNLWWSWNSRAENLFRRMDPTVWESVGHNPKKLLEQI
                     DYKRLLVLEEDEDFLRELNTVYRIFREYMDRPEDKTKPSVAYFSMEFGIHQCLKIYSG
                     GLGVLAGDYLKEASDSNLNIIGIGLLYRYGYFKQKLGPRGEQIAVYEAEEFSHLPVNP
                     VKDNEGNHLTVSLAWPGRTVKVRVWEAKVGKVSLFLLDTDFDENIPEDRTVTHHLYGG
                     DNENRLKQELVLGIGGVRALTAMGIKPDIFHSNEGHSAFISLERLRVLINDYHLTFDE
                     AIEVVRSSTLFTTHTPVPAGHDAFDEDLLRKYISHYHTRLNITWEKLMSLGRCESDSD
                     RKFNMSFLATRMSQEVNGVSKLHGEVSRDMFKKLWPGYLKEELFIGHVTNGVHYRTWL
                     APEWYNLYREITGKEDFDQTDRKMWEKIYEVDDSRIYEIKKGLKKALFANIRKKLQSE
                     MIELHVSPRTLLNVSTHLNENALTIGFARRFATYKRAYLLFKDLNRLEKIVNNPDRPV
                     QFIYAGKAHPNDGGGQELIRRIFEISQMPQFTGKIIFLENYDLELAKQLIRGVDVWLN
                     TPTRPLEASGTSGEKAVMNGTLHFSVLDGWWVEGYRAYAGWALPKKRTFENQDLQDDV
                     DAETIYNMLEYEIIPAYYSFNEQGIPVEWVSYIKNSMARIAPEFTMKRQIDDYFEKYY
                     IKLWERNKKIVANNFEKARELAAWKKKMIEEWDKIEVLNFKFEKPPEKIYRSGKDYKA
                     EILLNINSIPVENLGVELVITQMTQKGEYEFITSREFSLVSRKGGKCLYRAHIVPEKA
                     GSFFYGIRIFPKHKDLPHKQDFYLLRWID"
     gene            complement(5930..7972)
                     /locus_tag="PLG42_03275"
     CDS             complement(5930..7972)
                     /locus_tag="PLG42_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOU30266.1"
                     /translation="MKKSGFYLIDPWLKPYERIIESRYLKCITREIELAGERPLAEFA
                     SGHHYYGLHRNGSNWVFREWAPGAEAIYLTGTFSNWKDSTDYRLKRINTEGDWEIVLP
                     ADALSHGTLYKLSVHWKGGKGERIPSYATRVVQDEKTKIFSAQVWIPEEPYRWKNNSF
                     KPSGSVPLIYEAHVGMSSEEEKISTFREFADNIIPYIADTGYNTIQLMAIQEHPFYGS
                     FGYHVANFFAVSSRFGTPDDLKYLIDTAHGYGLAVIMDLVHSHSVKNINEGLGLFDGT
                     PSQYFHTGPRREHIAWDSLCFDYGKDKVLHFLLSNCRYWLEEYKFDGFRFDGVTSMIY
                     YDHGLGKNFVSYDDYFNGNQDEDALVYLYLANKVIHDFKPEAITIAEEMSGMPGIASS
                     TSSKGYGFDFRLSMGVPDFWIKLVKDTFDEYWDMGQLMHELTQHRPEEKVISYCESHD
                     QALVGDKTLIFRMIDADMYYGMTKEFHNLTVDRGIALHKMIRLLTFATAGGGYLNFMG
                     NEFGHPEWIDFPREGNNWSYKYARRQWSLVKNQNLKYSQLAEFDRQMLKLQTEGRVLE
                     NPSVIRIIENSHDKIIAFMRGDYLFVFNFHHVLSYTDYGIPVTGKFKVVLDSDDPAFG
                     GFNRIDRSIIYKSIKKSARLSINEPYRLYLYLPSRTALVFKKEPVKRVTDILVNPA"
     gene            8100..9032
                     /locus_tag="PLG42_03280"
     CDS             8100..9032
                     /locus_tag="PLG42_03280"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOU30267.1"
                     /translation="MKVHYGYNDLKFRNPVVTTGIFDGVHRGHRVVIEQIKKRAAELK
                     GETVVVTFHPHPRQVLNQNSQAIQLLTGIEEKISLIEKCGIDHVVLINFDRDLSLMSA
                     ADFFEKILCGKIGTKHFVAGFSHHFGRMAEGDKETIRSIADNYGISYEVLEPVKIGGI
                     IVSSSAVREALLTGRIEEANNLLGYDYFMNGTIIEGRKLGRKIGFPTANIKPDYPDKL
                     IPGNGVYAVEIYLKDKKYYGVMSIGYNPTVNRDQNIKSVEVNIFDFEGNIYGEKVCAV
                     FRYRIRDEMVFDNISELSRRIELDRQEALRLLKK"
     gene            9084..10499
                     /gene="ahcY"
                     /locus_tag="PLG42_03285"
     CDS             9084..10499
                     /gene="ahcY"
                     /locus_tag="PLG42_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOU30268.1"
                     /translation="MNVLTETLPYKVKDISLADFGRKEIEIAEKEMPGLLAVRRKYAA
                     EKPLKGARITGSLHMTIQTAVLIETLVELGAEVRWASCNIFSTQDHAAAAIAARGIPV
                     FAWKGETLEDYWWCTAQALSFPDGKGPTLIVDDGGDATLMVHLGYKAEKDPSILDKTP
                     ESREEEVILQTLKKIYSEDPHKWHRVVTELKGISEETTTGVHRLYQMLEKGELLVPAI
                     NVNDSVTKSKFDNLYGCRESLADGLKRAMDVMIAGKVVVVCGYGDVGKGCAKSMRAYG
                     ARVIVTEIDPICALQAAMEGFEVKTVEDTLDEGNIYVTATGNCDVITIEHMEKMKDQA
                     IVCNIGHFDNEIQVDKLNSYKGIKKINIKPQVDKYVFPDGHAIYLLAEGRLVNLGCAT
                     GHPSFVMSNSFTNQTLAQIELWKKNYKVGVYRLPKHLDEEVARLHLEQLGVKLTKLTD
                     KQAKYIGVSKEGPFKPDHYRY"
     gene            complement(10518..>11012)
                     /locus_tag="PLG42_03290"
     CDS             complement(10518..>11012)
                     /locus_tag="PLG42_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30269.1"
                     /translation="QKINRGLNDFTEKKLHNFILSIAILYLSCSKEKNDVIPDVYVDF
                     YISLDDPEFLSLNAPTNSVIVTYKTNNLGAPAAGYDSNGIIIYRADETQFYAYDRTCP
                     YDFAVNNLSIKVNIDGIYAVCPQCSTSYALPAGGSPASGPGRYPLKNYKTSFNGINVH
                     VWNH"
BASE COUNT         3537 a         2128 c         2394 g         2953 t
ORIGIN      
        1 tccctgtcag gagaatttat caaaaatggc agggatgaag taataaggat aagatgggaa
       61 aattcagccc gtcatgtccg ccagatttat gaagaggtga tttcgaaaca taaacaataa
      121 cagatatgag tgcagcagca aaaaaggcaa tatgcctctt ctttgaggtg caccagcctt
      181 tcagacttag acgttaccgg ttttttgacc tcgggattga ccactattac tatgatgatt
      241 ataccaatga gtctataatg cgaaaggtgg ctgaaagatg ttaccttcca gcaaacaata
      301 tcatacttga tctgatttca aaaaacaaga ataaatttaa agtctcgttt tccattaccg
      361 gtatagctct cgatcagttc agactttatg ctcctgaagt tattgattct ttcaaaagac
      421 ttgccgaaac tggctgtgtt gagtttctgg ctgagactga agctcattca ctggcatcac
      481 tgagcaacag ggctgaattt gaacgccagg taaacaatca caggcagtta ataaaagagt
      541 atttcggaat tgaacctcag gtattcagaa ataccgaaat gatttattcc gatcagatag
      601 gttcgtgggt tgcagatatg ggatttaaag ctgtccttac cgagggtgca aaacatgttc
      661 tggggtggaa aagtccgaat tatctgtatt gtaacacaat caacccccgg cttaaagttc
      721 tgctgagaaa ttttgtcctg agcgatgaca ttgcattcag attttcgaac cgcacatgga
      781 acgagtggcc tcttacagca gataaatacg cttcatggat taacaagctg gctccaaaaa
      841 gtgagattat aaatattttt attgattacg aaacttttgg tgaacataac cctaaggaat
      901 ctggtatttt cgatttttta taccatatgc cggcagcaat aatgaaaaaa acacctttta
      961 aattcatgac tccatcagaa gtggcgaata accttcagcc cgttgctgct ataaatattc
     1021 cgactcctat ctcatgggca gatgaggaaa gagaccttac cgcatggctg ggtaatgaac
     1081 tccagaatgc tgcatttgag aaattgtatg aactcactga cagggtcaat aagtgcgaat
     1141 atgaacagat aaagaaagac tggacatacc ttcaggcaag cgatcatttc tattatatgt
     1201 gtacaaaatt cttttccgat ggtatagtcc atgcctactt taacccttac gagagtcctt
     1261 atgaggcgtt tatgaattat atgaatatac tcagcgattt tgagataaga attaacagaa
     1321 tgtgtccagc tacagaacac gaaattgaaa tacttaaata cgattctcaa cttcgggcaa
     1381 aagatgaaat aatagcaaag caggcaaaag aaattgagac tcttaagaag aaacttgaaa
     1441 agttatcagg taaaaaaaca aaagccagct caaaaaagga gacagaggag aaggccgaaa
     1501 agaaagtaaa aacaaaacag aagaaaagca agacaggaga aaagagtctg gttgagctgg
     1561 ctgccgaggt agtcaaaaag acaaaactga aaaagtcaga atcgaaagga aagaaggcta
     1621 cctccagaaa aactgccgga aaagcctcaa aaaagaaaac atcatccgca aaatcaaata
     1681 aataatctca ccattaaatc aaatattttt aaaataattc tttatgaaag ttggatttct
     1741 ttttgaaaca agctgggagg tatgcaacaa ggttggaggg atacataccg taatttcaac
     1801 caaagccctg aacattgtaa atgaacttgg tgacaattac attttaatag gtcctgacgt
     1861 atggagggaa gatagcacca accctgagtt cattcctgat gattcgatgt ttattgactg
     1921 gaaaaacagg gcaatatctg aaggtctgaa ggtaaggacc ggaaggtgga acattgctgg
     1981 taggcctatt gtctttttac ttgattttac accttttttt ggtcagcaaa acgaaatttt
     2041 cgcacgcttc tgggaaacat acaaactcga cagtattaca gggcagtggg attatgttga
     2101 acctgcgctt ttcggatatg cagcaggaaa agtaatagaa agttttacca atttctatca
     2161 tgaacatcat gatatcgttg cccagtttca tgaatggatg actgggacag gtgttcttta
     2221 tcttaaaaag catgcgccct gggtggcaac agcatttaca acacatgcaa ctgtactggg
     2281 aaggtgtatt gcaggcaaca acagaccgct ttacggaaaa atgacggatt acaatccggt
     2341 gaatgtggca cgtgagttca atgtcatagc aaaacagtcg cttgaaaaaa taactgctgc
     2401 tgaagcagac gtatttacaa ctgttagtga acttacttca agagaatgcg ctcattttct
     2461 tggtaagaaa gttgacctta taactccaaa cggcttcgag aattcatttg tacccggccc
     2521 tgatgttttt aatgaaaaga ggcaaaaagc aagggagatg cttttcagag ttgcacaggc
     2581 agtgcttggt tatacgccat cgccagatgc aatgcttgtg gctaccagcg ggaggtatga
     2641 attcagaaac aagggaatag acatttttat tgactccctg ggtgagatca acagaaaagc
     2701 tgaaatacaa aaagaatgca taggatttat actagttcca gcatatcata aaggtccacg
     2761 gcaagatatt atagattttc tatataataa cggtcccgta ccttctggtg acagatatct
     2821 gacccatagc cttcattatc cggagccaga tgctatactg aacaggataa agagcaacgg
     2881 cctgacaaat gaccagaaga acagagtaaa ggtaatcttc gttccatcgt accttaatgg
     2941 gaatgacggc atattcaaca tgtcgtatta cgatcttctt ataggattcg atcttacagc
     3001 atttccatca tattatgagc catggggtta cacgccactg gaaagcatga tgttccgtat
     3061 acccaccctc acaaccaccc tttcgggttt cggattgtgg gttaaggaga atttcagcga
     3121 tacgggaaaa agcataagaa taataaacag aaccgatgag aatgaaccag aagtaatttc
     3181 atccatctgc tcttttattt cagaatttgc cacactagga gagcaggaaa ttgcagaagc
     3241 caggaacaaa gcttatgaaa tttcgaagac attgatgtgg gatagttttg taaagcatta
     3301 tttcacggca tacgacaggg cattgacggt aagctttgaa cgcaggaacg aaccgcgtga
     3361 gtttataaaa atggtagagg ctcctgtgct tgaaataaag aaacctcacc aggtaccggt
     3421 atggaaagat atatatgttc agagtgatgt tcctgagaaa ctggcagcat tgaaagagct
     3481 gtcaacaaat ctatggtggt cgtggaattc aagggcagag aaccttttca gaaggatgga
     3541 tccgacggtt tgggagagtg tggggcataa tcccaagaag ctccttgaac agattgatta
     3601 taaacggctt cttgttcttg aagaagatga agatttcctc agggagttaa atacagttta
     3661 tcggatattc agggaatata tggaccgccc tgaagataaa accaaaccat ctgttgcgta
     3721 cttcagtatg gaatttggta tacaccaatg cctgaaaata tactcagggg gccttggagt
     3781 ccttgcaggc gattatctta aggaggcaag cgattcaaac ctgaatatca taggaatagg
     3841 acttttatac cgttatggtt attttaaaca gaaacttggt ccgcggggag aacagatagc
     3901 ggtttatgaa gccgaagaat tcagccacct gccagtaaat ccggttaaag acaatgaggg
     3961 caaccatctt actgtttcac tggcatggcc tggccgaacg gtaaaagtca gagtatggga
     4021 agcaaaagtt ggaaaagtaa gcctgttcct tcttgataca gattttgacg agaatatacc
     4081 tgaagacaga actgttaccc accatctcta cggaggtgat aatgagaaca gactcaagca
     4141 agaacttgta ctcggtattg gaggagtaag ggctctgacg gcaatgggca taaaaccgga
     4201 tatcttccac agcaatgaag gacattcagc atttataagc cttgaaaggc tgagagtcct
     4261 tataaatgat taccatctga ctttcgatga ggcgattgaa gttgttcgtt cttcaacact
     4321 ctttaccaca catactccgg tcccggcagg acatgatgcc tttgacgaag acctgctgcg
     4381 taaatatatc tcccattatc atacacgtct taacattaca tgggaaaaac tgatgtcgct
     4441 cggaaggtgt gaatcagatt ccgacagaaa attcaatatg agttttcttg ctacccgtat
     4501 gtcgcaggaa gttaatggtg tttccaaact tcatggggag gttagccgcg atatgttcaa
     4561 aaaactctgg cccggttacc tgaaggaaga actgttcata ggacatgtta caaacggtgt
     4621 ccattaccgt acatggcttg caccggaatg gtacaacctg taccgggaga taaccggaaa
     4681 ggaagatttc gaccagaccg acagaaagat gtgggagaag atttatgaag ttgacgacag
     4741 caggatttac gagattaaga aaggactgaa aaaagccctc tttgcaaaca taagaaaaaa
     4801 gcttcagtcg gaaatgattg agctccatgt gagccccaga acgcttttaa atgtaagcac
     4861 ccatctgaat gaaaatgccc ttacaattgg atttgcaagg agatttgcaa catataagag
     4921 ggcatacctg ctgttcaagg atctcaaccg gctggaaaag attgttaaca atcccgacag
     4981 gcctgtacag tttatatacg caggcaaagc ccatccgaat gatggaggcg gacaggagct
     5041 gataagaaga atattcgaga tatcacagat gcctcagttc acaggtaaga tcatatttct
     5101 cgaaaattat gaccttgagc ttgcaaaaca actcatcaga ggtgtggatg tatggcttaa
     5161 tactccaaca aggccacttg aagcatcggg aacaagtgga gagaaggcag taatgaatgg
     5221 aacactgcac ttcagcgtgc ttgacggctg gtgggtcgaa ggctacaggg catacgcagg
     5281 ttgggcctta cctaaaaaac gtacttttga aaaccaggat ctgcaggatg acgtcgatgc
     5341 ggaaacaatc tataatatgc ttgaatatga aataatccct gcatattatt catttaatga
     5401 acagggaata cctgttgaat gggtgagcta tatcaaaaac tcaatggcgc gtattgcccc
     5461 ggagtttact atgaaaagac aaatagacga ttatttcgaa aagtactaca taaaactctg
     5521 ggaaaggaac aaaaaaatag ttgctaataa ctttgaaaaa gccagagaac ttgccgcatg
     5581 gaagaaaaaa atgatagagg agtgggacaa aattgaagtt ctgaatttta aatttgagaa
     5641 acctcccgaa aaaatttatc gttcaggaaa ggattacaaa gctgaaattc tgcttaatat
     5701 aaacagtatt cccgtagaaa atctgggagt agaactggta ataacccaga tgacccagaa
     5761 gggagaatac gaattcatta caagtcgcga gttttcactt gtcagccgta aaggaggaaa
     5821 atgtctttac agggcacata ttgtaccaga aaaggcaggc tctttctttt acggaatacg
     5881 aatattcccc aaacataaag accttccgca caaacaggat ttttaccttt tacgctggat
     5941 tgactaaaat atcagttact ctttttacag gctctttctt aaatacaagg gctgtacgtg
     6001 aaggaagata gagatacaat ctgtaaggct cattaataga gagccttgca ctttttttaa
     6061 tcgacttgta aataattgac ctgtcaattc tgttaaatcc gccaaaagca ggatcatcag
     6121 aatcgagcac aaccttgaat tttcctgtta caggtatccc gtaatctgtg taagataaca
     6181 cgtgatgaaa attaaatacg aacagataat cacctcgcat gaatgcaatt attttatcat
     6241 gtgagttttc tattattctg ataacggaag gattttccag tactcttccc tctgtctgca
     6301 gtttcaacat ctgcctgtca aattcagcca gctgggaata tttaagattt tgatttttaa
     6361 caagactcca ctgccttctg gcatatttgt aactccagtt gttaccttcc ctcggaaaat
     6421 caatccattc gggatgcccg aactcattac ccatgaaatt taaataccca ccgccagcag
     6481 ttgcaaaagt taaaagtctt atcattttgt ggagagcaat ccccctgtca acagtaaggt
     6541 tatgaaactc tttagtcata ccataataca tatcagcatc gatcattctg aaaataaggg
     6601 ttttatctcc aacaagtgcc tggtcgtgcg actcgcagta tgatataact ttttcttcgg
     6661 ggcgatgttg tgtgagttca tgcatcaatt gacccatgtc ccagtattca tcaaaagtat
     6721 ccttgacaag cttgatccag aagtcgggaa cgcccatcga aagacggaaa tcgaaaccat
     6781 aacctttaga tgaagttgat gatgcaatgc ccggcatacc gctcatttcc tcggcaatgg
     6841 tgatggcttc aggtttgaaa tcgtgaatta ccttgtttgc aagatatagg taaacaagtg
     6901 catcttcatc ctgattgcca ttgaaatagt catcgtaaga aacaaaattc ttccccagtc
     6961 catgatcata ataaatcatg cttgttacgc catcgaacct gaaaccatcg aatttgtact
     7021 cttcaagcca gtatctgcag tttgaaagca gaaaatggag tactttatct tttccataat
     7081 cgaaacagag tgaatcccat gcaatatgtt ctcttctcgg gccggtatga aaatactggg
     7141 aaggagttcc atcgaaaagc cccagacctt cattgatgtt tttaacacta tgggaatgaa
     7201 caagatccat tataacagca agtccgtaac cgtgtgctgt gtcgattaga tatttcaggt
     7261 cgtcgggtgt gccgaaccgt gaggagacag caaagaaatt tgcaacatga taaccgaaag
     7321 agccgtaaaa cggatgctcc tgaatagcca tcaactgtat ggtgttgtat ccggtatcgg
     7381 caatatatgg gataatatta tcggcaaatt ctctgaaagt gcttattttt tcctcttccg
     7441 acgacatacc cacatgagct tcataaatca atggaacgga ccctgaaggc ttaaaagaat
     7501 tatttttcca cctgtaaggt tcctcaggaa tccatacctg agcactgaat atctttgttt
     7561 tttcatcctg aaccactctt gtggcataag aaggaattct ttcacccttt cctcctttcc
     7621 agtgaacgga gagcttataa agggtaccat ggctgagggc atccgcggga agtacaattt
     7681 cccagtcgcc ttcagtatta atcctcttca gtctgtaatc ggtactatct ttccagttgc
     7741 tgaatgtccc ggtcagatat attgcttctg ctccaggcgc ccattccctg aaaacccagt
     7801 tgcttccgtt acggtggagc ccataataat gatgtcctga tgcaaactcc gccaacggtc
     7861 tctcccctgc aagctcaatt tccctggtta tacatttcag atacctgctc tcaattatcc
     7921 tttcataggg tttcagccag gggtctatta gataaaaacc tgattttttc atacaggcag
     7981 agttattcag ctaaagtata aaaaaatgat tcacatttga agctgaagcc ttgtttttcc
     8041 gttttataat cggtgataat acaggcactt tattaatttt gcccgtaaat tttaatgcgg
     8101 tgaaagttca ttatggatac aatgatctga aattcagaaa tccagtggta acaaccggta
     8161 tcttcgatgg tgtgcatcgt ggccatcggg ttgttattga acagattaaa aaacgggctg
     8221 ctgaattaaa gggtgaaact gttgttgtca cttttcatcc tcacccgcgc caggtactta
     8281 atcagaactc tcaggcaata cagcttctta caggtattga ggagaaaata tcgcttatag
     8341 aaaagtgcgg aatagatcat gttgttctaa ttaattttga cagagatctt agtttgatga
     8401 gtgctgcaga ttttttcgaa aaaattcttt gcggcaaaat aggcaccaaa cactttgtgg
     8461 ccggattcag tcatcatttc ggaagaatgg cagaaggaga taaggaaacc ataagaagca
     8521 tagcagacaa ctatggaatt agttatgagg ttcttgaacc tgtaaaaatt ggcggtatta
     8581 tagttagttc ttctgcagta agggaggctc tgctcacagg tagaatagag gaagccaata
     8641 atcttcttgg ttatgattat ttcatgaacg gaacaataat agaaggacga aagctgggaa
     8701 gaaaaatagg ttttcccact gcaaacatta aacctgatta ccccgataaa cttattcccg
     8761 ggaacggagt ctatgcggtt gaaatatatc tgaaagacaa aaaatattac ggagttatga
     8821 gtattggata caaccctacc gtcaacaggg atcagaatat taaatctgtg gaagtgaata
     8881 tatttgattt tgaaggaaat atttatggcg agaaagtttg tgcagttttc aggtatcgta
     8941 tccgcgacga gatggttttt gacaatattt ctgaactttc acgccgcatt gaactcgacc
     9001 gccaggaagc tttgcgtctt ctgaaaaaat aagtattccg aattttatta tctttgtatt
     9061 ctggtatata aatatgttaa atcatgaacg ttcttacaga aactcttcct tataaagtta
     9121 aagatatttc actggccgat tttggccgga aggagattga gattgccgaa aaagagatgc
     9181 ccggcctgct ggctgtacgc aggaagtatg cggctgagaa gcctcttaaa ggggcaagga
     9241 taaccggctc actgcatatg accattcaga cagctgtgct gatcgaaaca cttgtagagc
     9301 ttggcgctga ggtgagatgg gcaagctgca acattttctc aacgcaggat catgctgctg
     9361 cagccattgc tgccagaggc atacctgttt ttgcatggaa aggtgaaact cttgaagatt
     9421 actggtggtg cacagctcag gcactctcgt tccccgacgg aaagggaccc acactgattg
     9481 tagacgacgg tggtgatgcc actctgatgg tacatctcgg ctataaagcc gaaaaagatc
     9541 cttcaatact cgataaaaca cctgaaagca gggaagaaga agttatactt cagacgctga
     9601 aaaaaatata ttctgaagac ccacacaagt ggcacagggt agttactgaa ctgaagggta
     9661 tttcggaaga aacaaccaca ggtgtgcacc gcctctacca gatgctggag aaaggtgaac
     9721 ttcttgttcc ggcaataaat gtaaacgact cggttacaaa atcgaagttt gacaaccttt
     9781 atggatgccg cgaatcactt gccgacggac tcaaaagagc aatggacgtt atgatagcag
     9841 gcaaggttgt ggttgtgtgt ggttacggag acgtaggcaa agggtgtgca aaatcaatga
     9901 gagcgtacgg tgcccgtgtg attgtgactg agatagatcc aatatgcgcc ttgcaggctg
     9961 caatggaagg ttttgaagtt aaaaccgttg aggatactct cgacgaaggc aatatctacg
    10021 ttacagcaac gggcaattgc gatgttatca caattgagca tatggagaag atgaaggacc
    10081 aggcaattgt ttgtaacatc ggtcatttcg ataacgaaat tcaggttgac aaactgaaca
    10141 gctataaggg aatcaagaaa ataaacataa aaccacaggt tgacaaatat gttttccctg
    10201 acgggcatgc aatatatctt cttgctgaag gaaggctcgt taacctcggc tgtgccacag
    10261 gtcacccatc gtttgtgatg agcaactcat tcaccaacca gaccctcgca cagattgagc
    10321 tctggaaaaa gaattacaag gttggtgttt accggctgcc caaacacctc gatgaagagg
    10381 ttgcccgcct tcaccttgaa caacttggtg ttaaactgac aaaactcact gacaaacagg
    10441 ctaaatatat tggagtcagt aaagaaggcc ctttcaaacc agaccattac aggtattaat
    10501 agacactgaa ataaatttca gtggttccat acatggacat taattccgtt gaaggaagtt
    10561 ttataatttt tcagaggata tctacccggc cctgaggcgg gtgaaccacc agcaggaaga
    10621 gcatacgacg tgctgcattg cggacaaaca gcataaattc catcaatatt gacctttata
    10681 cttagattgt taactgcaaa atcgtaaggg catgttctgt cgtaagcata aaattgtgtt
    10741 tcatctgccc tgtaaattat aataccattc gaatcataac cggcagcagg agctccaaga
    10801 ttatttgttt tgtaagtcac aattacagaa ttagttggtg cgttaagtga aagaaattca
    10861 gggtcatcca gactgatgta aaaatcaacg taaacatctg gtataacatc gttcttttcc
    10921 tttgaacatg ataaataaag gatagcaatt gacaggataa agttgtgcaa ctttttttct
    10981 gtaaaatcat ttaagccacg attaattttt tg
//
LOCUS       DAOSNB010000045        11010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_5921_length_11010_cov_4.922136, whole genome shotgun sequence.
ACCESSION   DAOSNB010000045 DAOSNB010000000
VERSION     DAOSNB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5921_length_11010_cov_4.922136"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLG42_03295"
     CDS             complement(<1..208)
                     /locus_tag="PLG42_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30270.1"
                     /translation="MKNNRILKILAPLAVIMIIIAVIGKKQGWFGKALRVKVAVEEAA
                     IRSITEIITANGKIQPEKEVKITPD"
     gene            complement(219..1598)
                     /locus_tag="PLG42_03300"
     CDS             complement(219..1598)
                     /locus_tag="PLG42_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU30271.1"
                     /translation="MIVKRFISLVLLFSLISAGLYSQKKLWTLEECVRYALERNIQVK
                     QQELQTRVQKNALDLAKLKLLPTLAGNATHNYSFGRALDETTYEFTEHENVRTNNFYI
                     GGNLTIFSGLQNYHSIKKYRYELLASEEDLETIKNNIALSVAMGYLQILLNKELVNAT
                     ESQLLITRQQIDKTRKLVEAGSVAVGNLLQIEAQAAQEELQLVNLKNQLELSYLSLAQ
                     LLELETPAGFEIIIPEISIDTGYIVTGTVEDIFQVACKNRPEILSSEMRLRSSEFDLK
                     IAEGARSPRITMSHSFSTGYSDIRKKLLGIDPSTGPIYGKYTFADQIHDNVNYGIGFS
                     LSLPILNGWQINKNISNSKLSIENSRYALDATRKQLYKNIQQAYADAQAALKKYTASI
                     KAVQSAEESFRYSEQRFNVGLVTPVDYNAAKTQLLRAQSELAQSKYEFVFKIKVLDFY
                     KGIPITLPQ"
     gene            1894..2130
                     /locus_tag="PLG42_03305"
     CDS             1894..2130
                     /locus_tag="PLG42_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30272.1"
                     /translation="MKRTLYKEENLKRKNRLSLMLNNRELKALDIYCSRYRIKNKSEF
                     LRETIMKAIIKKFDDEYPSLWEEPTIPTLFSRKK"
     gene            2250..2663
                     /locus_tag="PLG42_03310"
     CDS             2250..2663
                     /locus_tag="PLG42_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU30273.1"
                     /translation="MYPQSIRIEATNKTPEIVLEQGIIFIKGRSIPEDPNNFYKPIYE
                     WVSKYISEWAGKTDIIFAFEFINTRSIKWIYTILKEMTELIYLPGNKLQVSWFYEEGD
                     EDMYDLGSIISALVRCPFRIIRIEDIDKCEITVLN"
     gene            complement(2660..3493)
                     /locus_tag="PLG42_03315"
     CDS             complement(2660..3493)
                     /locus_tag="PLG42_03315"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOU30274.1"
                     /translation="MFDINKNEIDSIIGLLETAGQEIIKVYRHNDFGTFLKEDRSPVT
                     RADLISDYIVKEGLKELTPEIPVFSEETIDTGNYLRLERNTLWILDPLDGTKEFIARN
                     DEFCISLALITDGKPVAGFIHAPVFKKTWFAVRGKGTWMLVAGEKTRVPAFKPDGPYR
                     ILRSRSHHSQAEESWLKKISLMYETEIITKGSAIKFCEIAEGYADLYLKSSLINEWDV
                     AAGHIIVEEAGGGIIEIATGKPPVYNKKDYHQHPFITYGVRGEKILNDILQILKIGRR
                     D"
     gene            3583..3655
                     /locus_tag="PLG42_03320"
     tRNA            3583..3655
                     /locus_tag="PLG42_03320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3616..3618,aa:Gln,seq:ttg)
     gene            3739..4161
                     /locus_tag="PLG42_03325"
     CDS             3739..4161
                     /locus_tag="PLG42_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOU30275.1"
                     /translation="MKSFIKITTLLIGFSLLFSTGCEKLNDEELLTDHIWKFKEITTN
                     STDQNVISIVAFVNALMTNSTMEFKKNGTFTLSMLNQSESGTWELSDDGKTLYTTDSN
                     GDTDEMTVVKLTKDDLILQGEEVDNTYGTYSVTMHWTK"
     gene            complement(4315..4614)
                     /locus_tag="PLG42_03330"
     CDS             complement(4315..4614)
                     /locus_tag="PLG42_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30276.1"
                     /translation="MNAYLVILAQSVATAAIEIIILLLCAGVVGFLSAWFYQKAYFTP
                     IIRKLEEEKEQLNRRIEELNSEISGLKSKISGMENLLAEKEREIQELKNQMKKEG"
     gene            complement(4755..5930)
                     /locus_tag="PLG42_03335"
     CDS             complement(4755..5930)
                     /locus_tag="PLG42_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30277.1"
                     /translation="MTEVLSQKLIKTFRRYVPSMKKKFNGMPSLEDLKKFIATAERIP
                     NNILYIPVGLRWLWLGLRYRSLTLPSVSNPMIETGGFMGESKASVLDMVGDEYRNWIA
                     RYILFHRDGGSADSNFQRALEALKIKGLEFPIVAKPDIGWNGYGVRFVENETQLLEYL
                     EQFPPGANLILQEAVNYDGEAGIFYIRIPGNEKGYIYSITLRYYPFVVGDGKSTLKQL
                     IENDERTKFRAHLYLGKSPKHVGFSNEDLERVPPEGELIRLAFIGSLRAGGLYRDGSH
                     LITPELTERFDTIARSMPEFYYGRFDIRFDSTDLLKRGEGFKIIEINGAGAEAIQAWD
                     PNVSLMKLYSEFFRAQSLLFKVADRNRKRGYKPATIKDFLKAIKKQNRLVDMYPPAS"
     gene            complement(5927..6490)
                     /locus_tag="PLG42_03340"
     CDS             complement(5927..6490)
                     /locus_tag="PLG42_03340"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOU30278.1"
                     /translation="MIISDPGILSWLKETIAIFFLTFLHEDAAIVAAGFAKVERGLPL
                     IYAYLPVYTGIVAGDILIYGLGRLARENRWLRSKIIGPKVEKVKTWLELNLVKVLIIC
                     RLTPGLLFPTFVACGWFRIPFWRFAIVSITAGALYSSLVLTIVILFGDLVLFKLGYWS
                     WAVLAAVVIIFAVRNYINQVRNDKQKI"
     gene            complement(6636..7961)
                     /locus_tag="PLG42_03345"
     CDS             complement(6636..7961)
                     /locus_tag="PLG42_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOU30279.1"
                     /translation="MEQQKQINPSRAIAIVVSNMIGTGVYTSLGLQAAGVHSALALIL
                     IWITGGLVALCGALTYGELAARIPRSGGEYIFLSKIYHPAFGFLSGFISMTIGFAAPL
                     AIAAIALGSYMNNIIPVPPMLTAISAILILTVLNSSSFRTGANFNFITTLLNITLILT
                     LCISGFIKGHHDDFELTFTRYDLKQVINPAFAVSLVYVSFAYSGWNSAAYITHQVRNP
                     VKNLPVIFITGTLIVMTLYTLLNFIFLYTVPVEQLSGKVDVAFLAARNIFGEFGGKFI
                     AVLISIGLIASINSLLILGPRVTQAIAEDYSALKFFGKENRNGSPFYATILLTVISLT
                     LIWTSTFEQIMTLIGFTLSIFTITSVGGLFVLRHRMKKNNENFYHTFGYPFIPLFFIL
                     VEGCMMVYVFANRPVQSLSGIGITLSGLLVWWLLIKRNRKAEILYKSTE"
     gene            complement(8115..8864)
                     /locus_tag="PLG42_03350"
     CDS             complement(8115..8864)
                     /locus_tag="PLG42_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOU30280.1"
                     /translation="MMSILWIISVIIKNVSIVDIFWGFGFVLTTWFYYFFTDGYELRK
                     LLISILVTIWGLRLSIYIAWRNAFKGEDFRYRNFRKKYGEKHYWWISFFQVFLLQGIL
                     MWLISSPLLGAQYYNRNNNLNLFDYIGLIVWITGLVFEAGGDLQLAKFKANPANNGKI
                     LDTGFWRYTRHPNYFGDSAVWWGYGLLSCAAGGYLPALGSILMTLLIIKVSGVAMLEK
                     SLKTKREGYEEYVKRTSAFFPWFPRKNKNSS"
     gene            complement(8921..10726)
                     /locus_tag="PLG42_03355"
     CDS             complement(8921..10726)
                     /locus_tag="PLG42_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOU30281.1"
                     /translation="MIKRNSEIVSELDGRNLYYLFVAGARKVIAHQVELNRINVFPVN
                     DGDTGSNLAATIRAVIESLYPDRSFKITADRIAESILINARGNSGIIFAQFFFGLSNE
                     TGNLNKIKIREFAETLKRSVNYVYEAVSNPVEGTMLTVIREWADFIYNTRQKYNDYTE
                     MLLGSYNVLKKSLSETKSKLKILAKNNVVDAGAKGFVLFIEGIIDYIRDRNLKELIRA
                     KAETAGLPKSEEMITEEVTYRYCTEALLKNTTIDQHSLMEILKEFGDSVVVAGNEKLR
                     RIHVHTSNPAEMFFVLKDYGTLAFQKADDMLRQSESVFKRKYRIALVTDSTCDLDESI
                     IDRFQIHIIPITINFGENCYLDKITLKPEQFYRMLDNCTDYPKSSQINENTFRNIFSH
                     LVSHYDSVISVNLSGKLSGTYFSAVKAAQAVSREFNKPVSVINSRGLSGSEGLLVLRI
                     AEAIEKNIPHEKIVEMAEEWVRKCKIYVKVNDIKYMIRGGRLSYAQGLIARLLHINPV
                     ISLDQEGKAIVLDKAFSRKANLEKVISHVKKSIESGSVWNYVVMHANNLQDAEWYIEQ
                     MRKLTGKEPVSVVNISPIIGANAGVGASAVALMTE"
BASE COUNT         3527 a         2211 c         1809 g         3463 t
ORIGIN      
        1 catccggtgt aatcttgact tccttttccg gctgaatctt cccatttgca gttattatct
       61 cagtaattga ccttattgcc gcttcttcga cagcaacttt aactcttaga gccttcccga
      121 accatccctg tttcttacct attactgcaa taattatcat tataacagcg agaggcgcaa
      181 gaatttttaa aatcctgtta tttttcataa aagcaaaatt attgtggtaa tgttatgggt
      241 attcctttat aaaaatcaag aactttaatt ttaaatacaa attcatactt cgactgggcg
      301 agttcagact gtgccctgag aagctgtgtt ttcgctgcat tataatcaac aggcgtcacc
      361 agaccgacgt tgaatctctg ctcagaatat ctgaacgatt cttctgccga ttgcaccgct
      421 tttatgctgg cagtatactt tttcagggca gcctgtgcat cagcataagc ctgctgaatg
      481 tttttataaa gctgtttccg cgttgcatca agtgcatacc tgctattttc aatggaaagc
      541 ttggaatttg aaatattttt attaatctgc cacccattca aaatcggcaa actgagtgaa
      601 aagccaattc cgtaattcac attgtcatga atctgatcgg caaatgtgta ttttccgtaa
      661 atagggcccg ttgagggatc aattcccagc agtttttttc ttatatctga atacccggta
      721 ctgaatgaat ggctcattgt aatacgcggg ctccgtgctc cttctgcaat tttcaaatca
      781 aattcactcg acctaagtct catttctgaa cttaaaattt caggcctgtt tttacatgcc
      841 acctgaaaaa tatcttccac agttcctgta acaatatagc cagtgtcaat actaatttcc
      901 ggtataatta tctcaaaacc agccggtgtt tcaagctcaa gaagctgagc taaactgaga
      961 taagataact caagctggtt tttaagatta acaagctgaa gttcttcctg cgctgcctga
     1021 gcttcaatct gcaaaagatt accaacagca acacttccgg cttcaacaag cttacgtgtt
     1081 ttatcaatct gctgacgggt aatgagtaac tggctttctg ttgcatttac taattcttta
     1141 ttcagaagta tctgcagata tcccatagct acactaagag caatgttatt ctttatagtt
     1201 tccagatcct cctcactggc aagcaactca tacctgtatt ttttgatcga atgataattc
     1261 tgtaatccgc tgaagatggt cagattacct cctatataaa aattattggt acgcacattc
     1321 tcatgctcgg taaattcata agttgtttca tcaagagctc ttccgaaaga ataattgtgt
     1381 gttgcattac ccgccagtgt tggtaaaagc ttcagtttag ccagatccag ggcatttttc
     1441 tgtaccctgg tctgcaattc ctgctgtttt acctgtatat tcctctccag tgcatacctg
     1501 acacattcct caagtgtcca caatttcttc tgggaatata aaccagcaga tattaagcta
     1561 aataatagaa ccagtgaaat gaaacgtttt acaatcatat ctacaataaa ttaatacagg
     1621 aaatgaggta cgaagataag aatttaataa tcgtctcaat tggctattta aagattttaa
     1681 tccaaaatca agttattgta gattcatttt gcatgactga tttaaaaata ctaattatga
     1741 gtggtagaat taaaagttgt aaagagttta tattattgat ttacaatttt ttaaaatatt
     1801 tgaaaatgaa tttgttaaaa acttatcttt taaaaataat aatacctgat gccaaccttg
     1861 taattttacg ccatattcaa agttaaagca gttgtgaagc gcacactgta caaagaagaa
     1921 aatcttaaac gtaaaaacag actatcgcta atgctaaaca acagggaact gaaagccctc
     1981 gatatttact gcagcaggta tcggattaaa aacaaatctg aatttctcag ggaaacaata
     2041 atgaaagcca ttataaaaaa attcgacgac gaataccctt cattatggga agaacctaca
     2101 attcccacac ttttttcaag gaaaaaatag aatgtcagaa taaactacat tgtttatttt
     2161 agttatgttc ggttaagtta aaagtatttt catactttta ttcaaaagaa ttatatctta
     2221 ttttagcatg ttaaaaacgt ttaaaattga tgtatcctca atcaataagg atagaagcta
     2281 caaacaaaac gcctgaaatt gttctggaac agggtattat tttcattaaa ggcaggtcta
     2341 ttcccgaaga tccaaacaac ttttacaaac ctatttacga atgggtttca aaatatattt
     2401 ctgaatgggc aggaaaaaca gatataatat tcgcttttga gtttataaat acgagatcta
     2461 taaaatggat atacactatt cttaaggaaa tgacagaact gatttattta ccaggtaata
     2521 aattacaggt atcatggttt tatgaagagg gggatgaaga catgtatgat cttggttcga
     2581 ttatttctgc acttgtcaga tgtcctttcc gtattatcag aattgaagat attgataaat
     2641 gcgaaataac tgttcttaat taatccctcc tgcctatttt cagaatctga agtatatcat
     2701 tcaatatttt ttcccctctt actccgtaag ttataaaagg atgctgatga taatcttttt
     2761 tattataaac cggcggctta cctgttgcaa tttctataat tccacctcca gcctcttcaa
     2821 caattatatg ccctgccgct acatcccatt cattaattaa acttgatttt agatacaaat
     2881 cagcataacc ctcggcaatt tcacaaaatt ttatagcact cccttttgtt attatttctg
     2941 tctcatacat tagacttatt ttctttaacc agctttcctc agcctgagaa tgatgcgaac
     3001 gacttctaag tatcctgtaa ggtccgtcag gtttaaaagc tggcactctt gttttttctc
     3061 ccgccaccaa catccaggtt ccttttcccc ttactgcaaa ccacgttttt ttaaatacag
     3121 gggcatgtat aaaaccagcc actggcttac catctgttat tagagccagg gaaatgcaga
     3181 actcatcatt acgggcaata aattccttcg ttccgtcaag ggggtcaaga atccatagag
     3241 tattcctttc caaccttaaa taattgccag tatctattgt ttcctcactg aaaacaggta
     3301 tttccggtgt taattctttc agcccttctt ttactatgta atctgatatc aggtcagccc
     3361 tggttacagg gctacggtct tctttcaaaa atgttccaaa atcattatgc ctgtatacct
     3421 taataatttc ctgacctgca gtttccagta aaccaataat tgaatctatt tcatttttgt
     3481 ttatatcaaa cattttcaga atattataat acatcagatc cacagaaagt aaaaataaca
     3541 atattttttt ctatttttgc atcagaaaac tgactataat agtgacccat ggtgtaattg
     3601 gtaacacgac ggattttggt tccgcagagt cctggttcga gtccaggtgg gtcaacataa
     3661 gttcttatta tacttaaatt cattaacctt aagataatat acaaagacaa tatccgttag
     3721 ttattaaaat ccattgtcat gaaaagtttt ataaaaatta ccacattact gattggcttt
     3781 tcattattat tttccacagg ctgtgaaaag cttaatgatg aagaacttct gactgatcat
     3841 atctggaagt tcaaggagat aaccactaac tccactgatc agaacgtcat ttccattgta
     3901 gcctttgtta atgcgctaat gacgaacagt acaatggaat ttaaaaagaa cggtaccttt
     3961 acactgagta tgcttaatca atcagaatca ggtacctggg aactgtctga tgatggtaaa
     4021 acactttata cgacagacag taatggggat acagatgaaa tgactgttgt caagctgaca
     4081 aaagatgacc ttatacttca gggtgaagaa gttgataata catatggcac ctattcagtc
     4141 actatgcact ggacaaaata attgcagccc acggtatata tttcttcaac ccaatttgaa
     4201 gttctggatt tatttgttgc ctatttgtgt cacctcatga aaggggtaac ataaatgcat
     4261 aaataaaaga atagccggtc tagtagaccg gctatatcat attaaccgtt aaaatcatcc
     4321 ttctttcttc atctggtttt taagctcctg gatttctctt tctttttcag ctaataaatt
     4381 ctccatacct gaaattttgc ttttcaagcc tgaaatttca gaatttagtt cttcaattct
     4441 acggtttaat tgctcttttt cttcctccag tttcctgata ataggagtaa agtatgcctt
     4501 ctggtagaac caggcggaaa ggaagcctac tacaccagcg caaagcagca aaataattat
     4561 ttcaattgca gctgttgcta ctgactgagc tagaataaca agatatgcat tcataatatc
     4621 agattttttg gggtttgtaa ttttccaata agtattcttt tttgtctttt atcaaagtta
     4681 agaaagtgat tgatttaaag caaaaaaatt tttgaatttc cggaaaaata accggaaaaa
     4741 tggcatttcc tcaattagct ggctggggga tacatgtcaa ccagccggtt ttgctttttt
     4801 attgctttaa gaaaatcttt tattgttgcg ggtttatatc cccgtttacg gttgcgatct
     4861 gccaccttga aaagaaggct ctgtgctctg aagaattcac tataaagctt catcagggaa
     4921 acattagggt cccaggcctg aattgcttca gctcccgcac catttatctc gataatctta
     4981 aaaccctccc ctcttttaag aagatctgta gaatcaaacc ttatatcaaa tctgccataa
     5041 taaaactcgg gcatgcttct tgcaatagtg tcaaatcttt ctgtaagctc cggagttatc
     5101 agatgactcc cgtcgcggta aagacctcct gcacgaagag aaccaataaa agccagacga
     5161 ataagttctc cctcaggagg aaccctctca aggtcttcat tgctgaaacc tacatgcttc
     5221 ggactcttgc caaggtaaag atgtgccctg aatttcgtgc gttcatcatt ttcaataagc
     5281 tgcttcagag tcgatttacc atctccaacc acaaaaggat aataccggag tgttatcgaa
     5341 tatatgtatc ctttttcgtt acccgggatt cttatataaa aaataccagc ttccccatca
     5401 taattaacag cttcctgcag tataagattc gcacctggag ggaattgttc cagatattcc
     5461 agcaactgtg tttcattttc aacaaatctt actccgtacc cgttccatcc aatatctggt
     5521 ttggcaacta ttgggaattc aagccctttt atttttaaag cctcaagagc ccgctggaaa
     5581 tttgaatctg cactgcctcc atccctgtga aaaagaatat atctggcaat ccagtttcta
     5641 tattcatctc ccaccatatc gagtacagaa gctttggatt ctcccataaa cccgcccgtt
     5701 tctatcatag gatttgacac agaaggcagg gtcaggcttc tgtatctcag accaagccat
     5761 agccagcgca atcccaccgg aatataaaga atattattag gaattctttc tgcagttgca
     5821 atgaactttt taagatcctc aagagatggc atgccattga atttcttttt catcgacggc
     5881 acataccttc tgaatgtttt aattaatttt tgcgataata cttctgtcat attttctgtt
     5941 tatcgttcct gacctgatta atatagtttc ttactgcgaa tattatgaca actgctgcaa
     6001 gtactgccca cgaccagtac ccaagtttaa aaagaacaag atccccgaac aggatcacta
     6061 tagtaagtac cagtgaagag tataaagctc ctgctgttat tgacacaatt gcaaatctcc
     6121 agaaaggtat acgaaaccat ccgcatgcaa caaaagtagg gaaaagcaac ccgggggtaa
     6181 gtctacagat tataagaact tttacaaggt tcaattcaag ccatgttttt actttttcca
     6241 ccttcggacc aataatcttc gacctgagcc atctgttctc acgagccagt ctgcccagtc
     6301 cgtaaatcag tatgtcaccc gctactattc ctgtatatac aggcaaatag gcataaataa
     6361 gcggcaagcc acgttcaact ttagcaaaac cggcagctac aattgccgca tcctcatgaa
     6421 gaaaagtcag aaagaatatt gcaattgttt ctttaagcca tgataatata ccgggatctg
     6481 aaataatcat gcttaataat tccccctctt gaaaaaatca aaatgcaaag atagattctt
     6541 ttatttatga taaaatatta atagtaagat tatcaggtat tttttaaaat ttaaccccgg
     6601 ttcaggaaca ggacttaaac aattatacag agtacttatt cagtgctttt atataaaatt
     6661 tctgcttttc tgtttctttt tattaataac caccatacta atagtccaga aagtgtaatt
     6721 ccaatgcctg acagcgattg tacaggtctg ttagcaaaca catataccat catgcaacct
     6781 tcaaccagaa tgaaaaataa aggaataaac ggataaccga atgtgtgata aaaattctca
     6841 ttatttttct tcattctgtg cctcagaaca aaaagtcctc caacagaagt tatggtaaag
     6901 atgcttaagg taaatcctat cagagtcatt atctgttcaa aggttgaggt ccatataagc
     6961 gttaaggaaa taactgttag aagtattgtg gcgtagaatg gggaaccgtt tctattttct
     7021 ttcccgaaaa acttcagtgc agaataatct tcagcaatag cctgagtaac tctcggaccg
     7081 agaataagaa gtgaattaat tgatgctata agtccgattg atatcaaaac agcgataaat
     7141 ttacctccga attccccgaa tatatttctg gcagcaagaa atgctacatc aacctttcct
     7201 gataattgtt caaccggaac tgtatacaga aaaataaaat tcagaagcgt atagagtgtc
     7261 ataacaatta aagttccggt tataaaaatt acaggtaggt ttttaactgg attcctgacc
     7321 tgatgggtta tataagccgc tgaattccat cctgaatagg caaatgaaac atatacaaga
     7381 gagactgcaa aggcaggatt tattacctgt ttcaggtcat accttgtaaa tgtcaattca
     7441 aaatcgtcgt gatgcccttt gataaaacct gaaatgcaaa gtgtaagaat aagtgttata
     7501 ttcagcaacg tggttataaa attgaaatta gcacccgttc tgaaactgct tgaattgaga
     7561 acagtaagaa taagaattgc agatatggct gtcagcattg gaggaacagg aatgatatta
     7621 ttcatgtatg aacccaatgc aattgccgct attgccagag gagcagcaaa gcctattgtc
     7681 attgaaataa atccggatag aaaaccgaaa gcaggatggt aaattttgga aagaaagata
     7741 tattcaccac ccgagcgtgg tattcttgcg gcaagctcac cataggttaa agctccacaa
     7801 agagcaacca gccctcctgt aatccagata agaatcaatg cgagagccga atgaactcct
     7861 gctgcttgaa gaccgagact tgtataaact cccgtaccaa tcatattaga aacaacgata
     7921 gcaatagcac gggacggatt aatctgcttt tgctgttcca aatcctgagt ttttgatagt
     7981 gctaaatgta acaaacaaaa taaaaaaaat gccctgatga aatgttaata aactcaaaat
     8041 tattgcaaga aaacagaaag acatattttc cgttaaataa gaattacgga tataattaat
     8101 tgattaaaaa agaattagct agagtttttg tttttacgtg gaaaccatgg gaaaaatgca
     8161 cttgttcttt tcacatactc ttcataacct tctcttttcg ttttaaggct tttttcgagc
     8221 attgccactc ccgatacttt tattatcaga agagtcataa gaatagatcc aagtgcagga
     8281 aggtaacccc ctgctgcaca agataacaat ccatatcccc accatacagc agaatctcca
     8341 aaatagttag ggtgccttgt ataacgccag aatccagtgt caagaatttt tccattgttt
     8401 gcgggatttg ccttaaattt tgcaagctgt aaatcacctc ctgcttcaaa aaccaaccct
     8461 gtaatccata caatcagccc tatataatca aagaggttaa gattgttgtt tctattgtaa
     8521 tactgagccc caagaagtgg tgatgaaatt aaccacataa gtattccctg aagcagaaat
     8581 acctgaaaaa aactgatcca ccagtaatgt ttctccccat atttcttcct gaaatttctg
     8641 tatctgaaat cttctccttt gaatgcatta cgccatgcaa tataaattga tagacgcaaa
     8701 ccccatatcg taaccagaat acttattagc agttttctta attcataacc gtcagtaaaa
     8761 aaataataaa accatgttgt caatacaaat ccgaaacccc agaaaatatc gactatgctg
     8821 acgtttttaa taattacaga aataatccac agaatcgaca tcataacaaa tatcggtaaa
     8881 agggcgtaca gaaaaatatc aagaaatgac attgttagta ttattcagtc atcaatgcca
     8941 ctgcagaagc acccactcct gcatttgcac caataatagg agaaatatta acaacagaaa
     9001 caggttcctt gccggttagt ttcctcattt gttcaatata ccattcagca tcctgcagat
     9061 tattggcatg catcacaaca taattccaga cagaacctga ctctatgctt ttctttacat
     9121 gagaaatcac tttttccaga tttgccttac ggctgaacgc tttgtccagc acaatggctt
     9181 tcccctcctg atcgagtgat attacaggat tgatatgaag cagcctggca ataagaccct
     9241 gagcatatga taaccggccc cctctgatca tatattttat atcatttacc tttacataaa
     9301 tcttacattt tcttacccat tcctcggcca tttcaactat tttttcgtga ggaatatttt
     9361 tttcaatagc ttcagcaatc ctgagaacta gaagaccctc agaacccgag agacctcttg
     9421 agttaataac actcacaggt ttattaaatt ccctgctgac agcctgtgct gcttttactg
     9481 cagaaaaata tgttccgcta agtttccctg aaagatttac tgaaataacc gaatcgtaat
     9541 gagaaacaag atgtgaaaat atattcctga aagtattttc attgatttga gatgatttag
     9601 gataatcagt acaattgtca agcatcctgt aaaactgttc tggtttaaga gtaatcttgt
     9661 cgaggtaaca gttttctccg aagttaatag ttataggtat gatgtgaatc tgaaaacggt
     9721 caataattga ttcgtcaagg tcgcaggtag agtcggtaac aagagctatt ctgtattttc
     9781 ttttaaacac cgactcactc tgccggagca tatcatcagc cttttgaaat gcgagtgtac
     9841 catagtcctt taaaacgaaa aacatttcag caggattgct cgtatgtaca tgaatgcgtc
     9901 taagtttttc attgcctgcc acaactacag aatccccaaa ttccttaaga atttccatta
     9961 aagaatgctg gtcgattgtt gtgttcttta gaagagcttc ggtgcaatat ctgtaagtca
    10021 cctcttcggt gatcatttcc tcactcttcg ggagccctgc tgtttctgct tttgccctga
    10081 taagctcttt gagattacga tcacgtatat agtcaataat tccttcgata aacagaacaa
    10141 aaccctttgc accagcatca actacattgt tctttgcaag tatctttagt tttgattttg
    10201 tttctgaaag agatttctta agcacgttat acgagccaag cagcatttcg gtataatcat
    10261 tatatttctg ccgggtatta taaataaaat cggcccattc ccttattacc gtcagcattg
    10321 ttccttcaac aggatttgaa acagcttcat aaacataatt aaccgagcgc ttcaaagttt
    10381 cagcaaattc ccttatttta attttattta agttcccggt ttcattactc aatccgaaaa
    10441 aaaactgggc aaaaataatt cctgaattac ccctggcatt tatcagaatc gattctgcaa
    10501 ttctatcggc agtaatttta aacgaacggt caggatagag cgattcaatc acagctctta
    10561 ttgtagctgc aagattagag cctgtatcac catcattaac cggaaaaaca tttatcctgt
    10621 tcagttcaac ctgatgagct ataacttttc ttgcaccagc aacgaagagg taataaagat
    10681 tacgtccatc aagctctgaa actatttctg aatttctttt aatcattatt acttttaaaa
    10741 atattttttc aaaggtaaga aatattaata acaaccattt ccaggtgatt atctgttttc
    10801 aaataacaat tatagaaact gacaactctt tccttggctg aaaaacaatt ttaaataatc
    10861 ataaaattaa cagacagaaa ggaattttgt tcaattgact tttaatgaaa gaataatcag
    10921 tgattcagta cataatataa tatccacccc caccgctgtt ttcaccaaaa cgcgggttga
    10981 ccacgttgtt gtatattgaa ccgaaagtgt
//
LOCUS       DAOSNB010000046        10804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6084_length_10804_cov_4.162248, whole genome shotgun sequence.
ACCESSION   DAOSNB010000046 DAOSNB010000000
VERSION     DAOSNB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6084_length_10804_cov_4.162248"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="nusB"
                     /locus_tag="PLG42_03360"
     CDS             <1..680
                     /gene="nusB"
                     /locus_tag="PLG42_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOU30282.1"
                     /translation="INNALKQYQESKRISWNINPDLPKILYNQMVKWEPYIKYMKDEK
                     TGYSEDKKFITKLVTEFFPSSEELLNAFEEQSIYWNDDIDYISLMVEKTLKKFKANSG
                     PEVPLMPLFKNEDDEHFVKHLFRQAILKTKQNIELIDRNTTNWEVERIALMDRLVMQL
                     AITEIVEFEEIPVKVTLNEYIEIAKQYCTSKSSNFVNGILDKIVRDMREKGLFRKSGR
                     GLLGETE"
     gene            696..1124
                     /locus_tag="PLG42_03365"
     CDS             696..1124
                     /locus_tag="PLG42_03365"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOU30283.1"
                     /translation="MIRTGQILFVIFAFSFLMSCRGKTSTGNALASSSDTAVIEFEEY
                     EHDFGKIIAGEKVATIFKFKNTGKGPLVLNSVTTSCGCTVSKYSTKPILPGDSGTIEV
                     IFDSGGYNGMQKKTVTVRSNASNPYVMLQIKAEVITGKNN"
     gene            1136..1465
                     /gene="yajC"
                     /locus_tag="PLG42_03370"
     CDS             1136..1465
                     /gene="yajC"
                     /locus_tag="PLG42_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOU30284.1"
                     /translation="MNSYLYFLAMAQPSGATGSGNALVTLMPLILVFVVFYFFMIRPQ
                     MKKQKELANYRNSLKKGDRVVTTGGIYGKVLEVKDNYVIMDAGGDVKLKVDKNALLKD
                     PTDIEQK"
     gene            1468..1956
                     /locus_tag="PLG42_03375"
     CDS             1468..1956
                     /locus_tag="PLG42_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30285.1"
                     /translation="MNKEKENFLDFFLLNMRKIFSRDLGIFLIFLLIAFILWYLNSLR
                     KETETTLKLQLNFINPPSGLVIANNSTGSLYLNLSGQGYSLLKFSMSKKNRILTIDLS
                     SVKLNRKSGNSSETEFYLLTSSVMKEYLNKAPSAEIKVLSVKPDTLFLFLVKNSSAGK
                     SE"
     gene            1973..2569
                     /gene="coaE"
                     /locus_tag="PLG42_03380"
     CDS             1973..2569
                     /gene="coaE"
                     /locus_tag="PLG42_03380"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOU30286.1"
                     /translation="MRLGVTGGIGSGKTIVCRIFSILGVPVFSADDAAKEIMDNDESI
                     KEKVNLAAGKNVYRNGILQRKELATIIFNNREILQKINSIVHPVLFEKFLEWEKMQHY
                     PYVIMEAAILFESGADKLVDRILTVTAPESERVKRILERNNTEINDVISRMKNQYDDN
                     FRTARSHYVVDNSGEKLVVPEILRIHNELLSLSVKKIN"
     gene            2569..3300
                     /locus_tag="PLG42_03385"
     CDS             2569..3300
                     /locus_tag="PLG42_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOU30287.1"
                     /translation="MGGFAKWIGGGLGWVLGGPIGGLLGFLFGSFVDESTSYPSHDTK
                     YVSTETTPGAFGVSLLVLVAAVMKADKRVLKSELEYVKQFFISQFGVESTREAMILLR
                     DLLKQDIPIKDVCKQIAKNMDYPSRMHLLHFLFGIAGADGKFGSEEVQVISKIAEYLG
                     ISSRDYTSIKNMFIPDTDAAYKILEIEPTATDEEVKKAYRRMALKYHPDRVSHLGEDF
                     KKAADEKFKKVNEAYEKIKKERNLV"
     gene            3403..3990
                     /locus_tag="PLG42_03390"
     CDS             3403..3990
                     /locus_tag="PLG42_03390"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOU30288.1"
                     /translation="MILKDYLAISGQHGLFKFIAQGRNAMIVEHLETRKRTTAFGSSK
                     VISLEDISIFTTGEEIPLSKVFDRIYDRENGGPAPDEKSDIKVLRTYFEEILPEYDRE
                     RVYESDIRKVFRWYNILQKLNLLVKEEPEKTEEKEEVKEEVVEGKMESKDSSEKTSST
                     KKLKSAAKSTEKTEEESAVKPAKSGSGKKKKTTEE"
     gene            4269..4871
                     /locus_tag="PLG42_03395"
     CDS             4269..4871
                     /locus_tag="PLG42_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvoyl-dependent arginine decarboxylase"
                     /protein_id="HOU30289.1"
                     /translation="MQNIVLRELHSGSSVEGMNLSLKGFIVGNRIPREYFVTTGIGES
                     DIAIHAGSYHLALKDAGIEMANIITYSSILPGMATQIPKPDKITHGEVMESIMAVANG
                     RKGERISAGIIYGWLKEKRTGQRYGGLVCEHNGNYTEEELEKLLRLSINELYTNGFDK
                     DYTLGDLNIIKRSFVPLKTYGTVLVALCFTSYFYPILETI"
     gene            4878..5714
                     /gene="speB"
                     /locus_tag="PLG42_03400"
     CDS             4878..5714
                     /gene="speB"
                     /locus_tag="PLG42_03400"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOU30290.1"
                     /translation="MNFGGNEVVYNYHESRIVILPVPYDETSTWLKGADRGPQAILEA
                     SVNLEFYDIETRSEVHKHGIYTMEPLTEKSSPEALVNTVFEKVSSLLNEGKFPVIIGG
                     NHTVSIGAANAAAAFFPSLTVLQFDAHADLRDSYEGSPYNHACVMARIREKSHTVQMG
                     IRSISAEEIHNADWNSIFPAHQLWYDKNLYKVALDLLKENVYITIDLDVFDPSLIPST
                     GTPEPGGPPYYDIMHFIREVAKNRNIVGFDVVELCPSPHNKAPDFVAAKIIYQTLSYI
                     FS"
     gene            complement(5711..6493)
                     /gene="mazG"
                     /locus_tag="PLG42_03405"
     CDS             complement(5711..6493)
                     /gene="mazG"
                     /locus_tag="PLG42_03405"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOU30291.1"
                     /translation="MNKRDIALKEFERLLEIMDRLRKECPWDREQSYESLRKLTIEET
                     YELTEAIFSGNEEGIKDELGDLMLHIVFYAKIGAERGTFDIADVLNHINRKLIYRHPH
                     VFGEIEVRNSDDVKKNWEKLKMDEKPEYKPVLSGVPSSLPAVTKAVRIQEKVRGAGFD
                     WEVPGQIWDKVLEEINELKVEVEEGDKENIESEIGDIFFSLINACRLYGIDPEAALEK
                     TNRKFINRFNYLERKTLAAGRSLNDMSLDEMNAIWEEAKKED"
     gene            complement(6500..7288)
                     /locus_tag="PLG42_03410"
     CDS             complement(6500..7288)
                     /locus_tag="PLG42_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOU30292.1"
                     /translation="MPVITLTTEWRSDDFYNGIIKGKLCSLCPGVTIIDNATGIPAFN
                     ISHASFVIRNTYSHYPPGSVHIICVHTEGNEEKRHLAVRSGEHYFIGTDNGIFYLILN
                     REPDEVVILGNKDETDEIDIFVSAAAMILKGKTLKEIGMPAVRINEKIPLRATIEKDI
                     ITGSVIFIDSYGNAISNITREIFDRVFSGKRFRILIQSTRNSINRISKRYDNEPVGEL
                     CARFNMLDLLEISINGAKVSELLNLTNGSVIRVELAPEEKILKH"
     gene            7390..8406
                     /locus_tag="PLG42_03415"
     CDS             7390..8406
                     /locus_tag="PLG42_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOU30293.1"
                     /translation="MAKGRQKDIARTTEKVIYLDDIEPVILFGTNNVLLNKIRNIFPQ
                     LKIVARGNELKCIGEENEIMLFSERLNDVIDYYRKYHRLDDDVIERMLTDEEHVKKAI
                     EGSFEDVIVYGANGRPIRARTIHQLQLVNDYKTNDLIIADGPAGTGKTYTSIALAVRA
                     LKNREVKKIVLTRPAVEAGERLGFLPGDMKEKLDPYLQPLYDALHDMLPYKRLETWME
                     DGTIQIAPLAYMRGRTLENAFVILDEAQNATVSQLKMFLTRMGLCSKFIMTGDTTQID
                     LPRKNDSGLLQAMRILSNIEGISIIRFDERDIVRHRLVKMIIRAYEKEEKNSGKTSDS
                     KDDV"
     gene            8425..9381
                     /locus_tag="PLG42_03420"
     CDS             8425..9381
                     /locus_tag="PLG42_03420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOU30294.1"
                     /translation="MKNTVLRTDFKFPGLKSIYHGKVRDVYNINDEILLMVVTDRISA
                     FDVVLPRGIPYKGQVLNQITSKFLDLTSDIIPNWKIASPDPNVTAGIICKPYPVEMIV
                     RAYLTGSSWRTYKSGKREICGIPLPEGMREHQKFDEPLITPTTKAEQGLHDEDITPKE
                     IVEKGLVSEEEYTLIEKYSLEIFKRGSEIATKNKLILVDTKYEFGKKDGKIYLIDEVH
                     TPDSSRYFYAEDYQERFSKGLPPRQLSKEFVREWLMANGFMGRTGEKVPDMTDEFVNE
                     VSERYIELYEKITGEKFIREELSNINERIYENCMKFLKEYYG"
     gene            9421..>10804
                     /locus_tag="PLG42_03425"
     CDS             9421..>10804
                     /locus_tag="PLG42_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOU30295.1"
                     /translation="MEKLKDELKITSLFVSVFVFLLVNGITVSAQVVVEKSKEKVIIS
                     GNQYYIHIVKKGETAFSIAKAYGITVDELVKNNPSAATVLKEGQALRIPVVETISDEQ
                     KKTEVKEQQRDESKFFYHKIRPGDTVYSLAKSYGVSVDEIVQSNPGVDINKLPVNAEI
                     AIPRRSLTTTSKDFEVSEKEYLYHRIMKGETLASIAEKYGITIRELRRENRGIIFPRV
                     NDSLRIPVQRVVDFKIKADTVAADTIKTVPDEDFEIPSEIIPVRNLKGEFNVGVLLPL
                     YTRESSIRAEIDSSQVIKGKRIYKTIYKPEHWLYPSSIQFLELYNGILVAADTLRNMG
                     LDINIYAFDTSTEEYGVDNLIKTDRLKKMDMIIGPVFTDELIKVADYLRDSDIPVISP
                     VPLRSNIPLKNNPEVFMTIPSIKVVQNLIVRRASQFKNSNFVFIHNDRTWSDSDVINF
                     RNMLLREITSN"
BASE COUNT         3572 a         1928 c         2205 g         3099 t
ORIGIN      
        1 ggattaataa tgctctaaaa caatatcagg aatcaaaaag aatatcatgg aatattaacc
       61 ccgacctccc aaaaattctt tataaccaga tggtgaaatg ggagccatac ataaaataca
      121 tgaaagatga gaagacagga tattctgagg acaaaaagtt cataactaag ctggttactg
      181 agtttttccc ctcatcagag gaacttctta atgcattcga agaacagagt atttactgga
      241 acgatgatat tgattatatt tcgctgatgg ttgaaaagac cctgaaaaaa tttaaagcga
      301 attccggtcc agaagtgcca ctgatgccac tttttaaaaa tgaagacgat gaacattttg
      361 ttaaacatct ttttcgccag gcaattctca agaccaaaca aaatatagaa cttattgaca
      421 gaaatacgac aaactgggag gttgaacgta tcgcactgat ggatagactt gttatgcaac
      481 tggctataac tgagattgtt gaatttgaag agataccggt taaagtaact ttaaatgaat
      541 atattgaaat tgccaagcag tactgtacat caaaaagcag taactttgtc aacggaattc
      601 tcgacaagat tgtaagggac atgagggaga aaggactttt ccggaagagt gggcgagggc
      661 tgcttggaga aacggagtga taatatttaa aaactatgat aagaacaggt caaatacttt
      721 ttgtcatttt tgcgttttct tttcttatgt cgtgcagggg caaaacatca accggtaatg
      781 ctttagccag ctcttctgat acagccgtta tcgaattcga agaatatgaa cacgactttg
      841 gaaaaattat tgccggtgaa aaagttgcaa caatcttcaa attcaaaaac accgggaaag
      901 gtccgcttgt gctaaattca gttactacaa gctgtggatg tactgtttcg aaatacagca
      961 caaaacctat cctgcctggc gactcaggta caattgaggt catatttgac tcgggtggat
     1021 acaatggaat gcagaaaaaa acagtaacgg tacggtcaaa cgcttcaaac ccttatgtta
     1081 tgttacagat aaaagcagaa gtaataacag gtaaaaacaa ttaataatca gaaacatgaa
     1141 cagttatctt tattttttag cgatggcaca gccttcaggt gcaacaggat caggcaatgc
     1201 gcttgttacg ttaatgcccc tgatcctggt ttttgtggtt ttctattttt ttatgatcag
     1261 gccacagatg aaaaaacaga aagaacttgc caattacaga aattctctca aaaagggaga
     1321 ccgcgttgtg actaccggcg gtatctatgg caaggttctt gaagtgaagg ataattatgt
     1381 aattatggat gcaggtggtg atgtgaaact caaagtggac aaaaacgcat tacttaagga
     1441 ccccactgat attgaacaga aataataatg aataaagaaa aagaaaactt cctggatttt
     1501 ttcctgctga atatgaggaa aatattcagc agggatcttg gaatttttct tatttttctt
     1561 cttatagcat ttattttgtg gtaccttaat tccctgagga aggaaacaga gacaactctt
     1621 aagcttcagc ttaattttat aaatccgccc tctggtttgg taattgcaaa taattctacc
     1681 ggtagtttat atcttaacct gtcagggcag ggttattcgc tgcttaaatt cagtatgagc
     1741 aagaaaaacc gaattttgac aatagatctt tcatcggtaa aattgaacag aaagtcgggg
     1801 aattcttcag aaactgaatt ttatttgctt acctcatcgg ttatgaagga gtatttgaat
     1861 aaggcacctt ctgctgagat aaaagttttg tcagtaaaac ctgatacact ttttcttttc
     1921 cttgtaaaaa attcatctgc aggcaaatca gaatgaagaa aatcaacttt caatgagact
     1981 tggtgttacc ggtggtattg gaagcggaaa aacaattgta tgcagaatat tcagtattct
     2041 gggtgtaccg gtcttttctg ccgatgatgc cgcaaaggaa ataatggata atgatgagtc
     2101 aatcaaggaa aaggttaatc ttgctgcagg aaagaatgtg tacaggaatg gaatacttca
     2161 aagaaaagaa cttgccacta ttatttttaa caacagggaa atactgcaga agataaacag
     2221 tattgttcat cctgtgttat ttgaaaaatt tctggagtgg gaaaaaatgc agcattatcc
     2281 atatgtaatt atggaagctg caattctctt cgaaagcggt gcagataaac ttgttgacag
     2341 gatactgacg gtaactgcac cagagagtga acgtgtaaag aggattttgg agcggaataa
     2401 tacagaaata aacgatgtta tttccagaat gaagaatcag tatgatgaca acttcagaac
     2461 agcaagatca cactatgttg ttgataattc aggtgaaaaa ttggttgtgc ctgagatttt
     2521 aagaattcac aatgaattac tttctttgtc agttaaaaaa atcaactgat gggaggtttt
     2581 gcaaaatgga ttggaggagg actcggatgg gttcttggag gacctatagg agggttactt
     2641 ggttttctct ttggatcatt cgttgatgaa tcaacctcct atccttcgca tgatacaaag
     2701 tatgtttcaa ctgaaaccac ccctggggca ttcggtgtaa gcctgctggt acttgtggca
     2761 gcagtgatga aagccgataa acgagttctt aaatcagaat tggaatatgt aaagcagttt
     2821 tttatcagcc agttcggtgt ggaatcgacc cgagaggcaa tgatactgct gcgggatctg
     2881 ctcaaacagg atataccaat aaaagatgtc tgcaaacaga ttgcaaaaaa tatggactat
     2941 ccgtcgcgta tgcatcttct gcactttctc tttggcatag ccggtgccga cgggaaattc
     3001 gggtcagaag aagtacaggt cataagcaaa atagcagaat acctgggaat ttccagtcgt
     3061 gattacactt caataaagaa tatgtttatt cctgataccg atgcagctta taaaattctt
     3121 gagattgaac ccacggcaac tgacgaggaa gtgaagaaag catacaggcg aatggctttg
     3181 aaatatcacc ccgacagggt gagccacctc ggagaagatt ttaagaaggc tgcagacgaa
     3241 aaatttaaaa aggtcaatga agcatacgaa aaaatcaaaa aggaaaggaa tctcgtgtga
     3301 ttcataatta atttagatat tcaaataaaa acatgaagga aaaaagaata ttaattgaaa
     3361 tatcacggta caaaaactaa atttgcacaa aaataattga gtatgattct taaagactat
     3421 cttgcaatat cgggtcagca cggtttgttc aagttcattg cgcaggggcg caatgcaatg
     3481 atagtagaac accttgaaac caggaaaaga actacagctt tcggatcttc aaaagtaatt
     3541 tcactcgaag acatttcaat ttttacaacg ggcgaagaga tacctctgtc gaaagtgttt
     3601 gacagaattt acgaccgtga aaacggaggt ccggcacctg atgagaagtc cgatataaaa
     3661 gttctcagaa catattttga agagattttg cctgaatacg accgggaaag agtttacgaa
     3721 tctgatataa gaaaagtatt caggtggtat aatatattgc agaaacttaa ccttcttgta
     3781 aaagaagagc ctgagaaaac agaggaaaaa gaagaggtga aagaagaagt ggtagaagga
     3841 aaaatggaaa gtaaagattc ttctgaaaag acatcttcaa ctaagaaatt aaaatctgca
     3901 gcaaagtcaa cagaaaagac tgaagaggag agtgctgtaa agcccgcaaa atcaggatct
     3961 ggaaaaaaga agaaaacaac cgaagaataa taaagtttct ttcaaaaatc cttacagaga
     4021 taaaatatct tttattgagt tttatctgtt aatttctttt atcaatcgtg aaagatccag
     4081 aatagttaat atgttaataa tcaataaatt ataaaattga ttttaatcgc tttttgataa
     4141 aaagaggtct tttgtgtaaa aattttttcc agaatataaa aaaaatatta atattgattt
     4201 atatttgcat cccaaaatta aacaggtaaa aattagtatt aacaacccaa ttgtaattta
     4261 tgacgtagat gcagaacata gttttgagag agttacactc cggtagttcc gttgagggga
     4321 tgaatttgtc cctgaaagga ttcattgtcg ggaaccgtat acccagagaa tactttgtta
     4381 ctactggtat cggtgaaagc gacatcgcaa ttcatgcagg atcttaccac ctggctctca
     4441 aagatgccgg tatcgaaatg gccaacatta tcacctattc ttcaattctt ccgggcatgg
     4501 ccacccaaat tcccaaacct gataaaatta cccatggcga agtgatggag tctattatgg
     4561 ctgtcgccaa tggcagaaaa ggagagagaa tttctgcggg aattatttat ggatggctga
     4621 aagaaaaaag aaccggtcag aggtacggtg gtctggtctg cgaacacaac gggaattata
     4681 ccgaggagga gctggaaaaa ttgctccgcc tgagtataaa cgagttgtat accaatgggt
     4741 tcgataaaga ctacactctt ggtgatttga atatcattaa gaggtctttc gtgcccctca
     4801 aaacgtatgg gactgttctg gtagcgctct gttttaccag ttacttttat ccaatcctgg
     4861 agaccatctg atgctttatg aatttcggag gcaatgaggt agtttataac taccatgaat
     4921 cccgtattgt catacttccg gtaccgtatg acgaaaccag tacatggtta aagggggctg
     4981 acagagggcc gcaggctata cttgaagcat cggtaaacct cgaattctat gatattgaaa
     5041 cccgctccga agttcataaa catggaatct atacaatgga gcctctgacg gaaaagagct
     5101 cgccagaggc tcttgttaat actgtatttg aaaaagttag ttcacttctt aatgaaggaa
     5161 agttccctgt tattatagga ggaaaccata ctgtaagcat cggagctgcc aatgcagctg
     5221 ctgctttctt cccttcactg acagtacttc agtttgatgc ccatgccgac ctgcgcgaca
     5281 gttatgaagg ctcaccctac aaccatgcat gcgtaatggc aagaatacga gaaaagtcac
     5341 atacagttca gatgggaata agaagtattt ctgctgaaga gattcataat gccgactgga
     5401 actcaatttt tcctgctcat caattatggt atgataagaa cctttacaaa gtggcacttg
     5461 acttacttaa agaaaatgtc tatatcacaa tagaccttga tgtatttgac ccctccctta
     5521 taccatcaac aggaaccccc gaacctggcg gaccaccata ttatgatatt atgcacttca
     5581 tacgcgaagt ggcaaaaaac agaaacatag tgggattcga cgttgttgaa ttgtgtccct
     5641 caccccataa taaagctcct gactttgtag cagcaaaaat tatttaccag accttgagct
     5701 atatttttag ttaatcttcc ttctttgcct cttcccatat agcattcatc tcatcaaggc
     5761 tcatatcatt caatgagcgg cctgcagcaa gtgtctttct ctccagataa ttgaaacggt
     5821 tgataaattt tctgtttgtt ttttcaagag cagcttcagg atcaatgcca tagagcctgc
     5881 atgcattgat aagactgaaa aaaatatccc ctatttcaga ctcaatattt tccttgtctc
     5941 cttcttcaac ttctaccttt aattcgttta tctcttcaag tactttatcc cagatctgcc
     6001 cgggaacttc ccagtcaaaa cctgcacctc ttactttctc ctgtatcctt acagcttttg
     6061 ttacagccgg cagggaagat ggtacacccg agagaacagg tttgtattca ggtttttcat
     6121 ccatcttcag cttttcccag ttctttttaa cgtcatcaga gtttcttact tcaatttctc
     6181 cgaatacatg aggatgcctg taaataagct tcctgttgat gtgatttaat acatctgcta
     6241 tatcgaatgt ccctctttca gctcctattt ttgcataaaa aactatatgt aacattaaat
     6301 cgcccagctc atccttgata ccttcttcat ttcccgaaaa aattgcctct gtcagttcat
     6361 atgtttcttc aattgtcagc ttcctgaggc tctcgtaact ttgctctcta tcccaggggc
     6421 attccttcct gagcctgtcc attatctcga gcagtcgctc aaattctttt aatgcaatat
     6481 ctcttttatt cattgtcaat tagtgtttca gaattttttc ttcaggcgca agttcaaccc
     6541 ttattacaga cccattggtg aggttaagaa gttcagatac ttttgctcca ttaattgaaa
     6601 tttccagcag gtcgagcatg ttgaatctgg cgcacagttc tccgacgggt tcattgtcgt
     6661 atcttttact tatcctgtta atagagtttc tcgtgctctg tatgagaatt ctgaaccttt
     6721 ttccggagaa aaccctgtcg aaaatttctc gtgtaatatt ggaaattgca tttccgtatg
     6781 aatctataaa tatcacactg cctgttatta tgtctttttc aattgttgcc cgtaaaggaa
     6841 ttttttcatt gatacgtact gcaggcattc cgatctcctt cagtgttttc ccttttaaaa
     6901 tcattgcagc agcagaaaca aaaatatcta tttcatccgt ttcgtcttta ttcccaagta
     6961 taacaacttc atccggctca cggttgagaa tgagatagaa aatcccattg tcggtaccga
     7021 taaaataatg ttcgccagat cttacagcca gatgcctctt ttcttcattt ccttcagtat
     7081 gtacacaaat aatgtgaact gaacccggag gataatgaga ataagtattc cggattacga
     7141 aagatgcatg ggaaatgtta aatgcaggta ttccggtggc attatcaata atggttacac
     7201 ccggacataa actgcagagt ttgcctttga taattccgtt gtagaaatca tcacttctcc
     7261 actcggttgt aagtgttatc accggcatgg gaaaaacttt tgacaaagat aatcttctaa
     7321 cgaaaaataa gcttactttg tagtttctaa ataaatattt tttcaaaagc atttttaaat
     7381 ctgaaagctt tggcaaaagg cagacagaaa gatattgcac gtacaacaga gaaggtaata
     7441 tatcttgatg atattgaacc tgttattctt tttggcacaa ataatgtcct gttgaataaa
     7501 ataagaaata tttttccgca gttgaagatt gttgccagag gaaatgaact taagtgcatc
     7561 ggtgaagaaa atgagatcat gttgttttct gagaggctta atgatgtaat tgactattac
     7621 agaaaatatc acaggcttga tgatgatgtt atcgaaagaa tgcttaccga tgaggaacat
     7681 gttaaaaagg caatagaggg cagttttgag gatgtgattg tttacggagc aaatggcagg
     7741 ccgataaggg cacgtacaat tcatcagctt cagctagtga atgattacaa aacaaatgat
     7801 ctgattatag ctgatggacc tgcagggact ggaaaaacct atacatccat tgctcttgca
     7861 gtacgagccc tgaaaaacag ggaggtgaaa aagattgtac ttacgcgtcc tgctgttgag
     7921 gcgggggaaa gactcggatt ccttcccggc gacatgaagg aaaaacttga tccgtacctt
     7981 cagccactct atgatgccct tcacgacatg ttaccctata agcggctgga aacatggatg
     8041 gaagatggaa caattcaaat tgcgccactg gcatatatga gaggccggac acttgaaaat
     8101 gctttcgtga tacttgatga agcccagaat gccactgtta gccagttaaa aatgttcctt
     8161 acaaggatgg ggttatgctc taagtttatt atgacaggcg ataccacaca gatcgacctg
     8221 ccccggaaaa acgattcggg acttctgcaa gcaatgagaa tattgtcgaa catcgaagga
     8281 atatctatca tcaggttcga cgaacgtgat atagtcaggc accggctggt taaaatgatt
     8341 atcagggcat acgaaaaaga agaaaaaaat tcaggaaaaa cttctgattc aaaagatgat
     8401 gtttaacatt aaccttttaa aaccatgaaa aatacagttc tcagaaccga tttcaaattt
     8461 ccaggcttga aaagcatcta ccacggaaaa gtgcgtgatg tttataatat taacgacgaa
     8521 atacttctaa tggttgtaac cgacaggatt tcagcatttg atgttgttct tcccagaggc
     8581 atcccataca agggacaggt tcttaatcag ataacttcga aatttcttga tcttaccagt
     8641 gatattatac caaactggaa aatagcctca cccgacccca atgtgactgc aggaataatc
     8701 tgcaagcctt atcctgtgga aatgatagtc agagcttatc taactggaag ctcatggcga
     8761 acatacaaat cgggtaaaag ggaaatatgc ggcataccct taccggaagg aatgagagaa
     8821 catcagaaat tcgacgagcc tctgattacc cctactacaa aagctgaaca aggacttcat
     8881 gatgaggata ttacaccgaa agagatagtt gaaaaaggtt tggtatctga agaagaatac
     8941 actctaattg agaaatattc acttgaaatt ttcaaaagag ggtctgaaat tgcaacaaaa
     9001 aacaaattga ttcttgttga tactaaatat gaattcggaa agaaagacgg aaagatatat
     9061 cttatagatg aagttcatac tcccgattct tcaaggtatt tttatgccga agattatcag
     9121 gaaagattca gcaagggatt accgccaagg cagctttcga aagaatttgt gagggaatgg
     9181 ctgatggcaa atggtttcat gggtaggaca ggggaaaaag tacctgatat gacagatgaa
     9241 tttgttaatg aagtgtcaga aagatatatt gaactttatg aaaaaattac aggggaaaaa
     9301 tttatacggg aagaactgag caatataaat gaaagaatat acgaaaactg catgaagttt
     9361 ctcaaagaat attacggata attacactgt ttttttaaat aattaaaatt gtattcctga
     9421 atggagaagt tgaaggatga gttgaaaata acaagcctgt ttgtatcagt ttttgtgttt
     9481 cttctggtta acggaattac cgtctcagcc caggttgtgg ttgagaagtc gaaagaaaaa
     9541 gtaattatat caggcaacca gtattatatc cacatagtta agaaagggga gacagctttt
     9601 tcaattgcaa aagcctacgg tattacggtg gatgaactgg taaagaataa tccatcagct
     9661 gctactgttc ttaaggaagg tcaggcactg cgcataccgg ttgttgaaac aatatctgat
     9721 gagcagaaaa aaacagaggt taaggaacaa caaagagatg agtcaaagtt tttttaccac
     9781 aaaatacggc cgggagatac tgtctattca cttgcaaaaa gttatggtgt ttctgttgat
     9841 gaaattgtcc agagcaatcc gggcgttgac ataaataaac tgcctgtcaa tgccgagata
     9901 gcaataccca ggcggagcct tacaacaacc tcaaaggatt ttgaggtttc ggaaaaggaa
     9961 tatctttacc acagaataat gaaaggtgaa acgctcgctt ccattgctga aaaatacggc
    10021 attacaatac gggagttgag aagggaaaac cggggaataa ttttcccgcg tgttaatgat
    10081 tcactaagaa tacctgttca aagagttgtc gactttaaga taaaagcgga tactgttgct
    10141 gcggatacaa ttaaaacggt gccagatgaa gattttgaaa ttccttcaga aattataccc
    10201 gtcagaaatc taaagggtga attcaacgtt ggagtgctcc tccctttata taccagggaa
    10261 agttcaatac gtgccgaaat tgattcatca caggtgatta aaggtaagcg tatctataaa
    10321 acaatttata aaccggaaca ctggttgtac ccctcaagta ttcagtttct tgaattatat
    10381 aacggtattc ttgtagctgc tgatacactg aggaatatgg ggcttgatat aaatatttat
    10441 gcttttgata caagtactga agaatatggt gttgataatc ttataaaaac agaccggctc
    10501 aaaaagatgg acatgataat tggccctgta tttacagatg agctcattaa ggttgctgat
    10561 tatctgagag attctgatat acctgtaatt tcgcctgtac cgctgcgtag taacattcct
    10621 ttaaaaaata atccggaagt atttatgacc attccctcta ttaaggtggt tcagaatctt
    10681 atcgtccgac gtgccagtca gtttaaaaac agtaattttg tttttattca taacgacaga
    10741 acatggtctg attcagatgt aattaatttc cgcaatatgt tattaaggga aataacatca
    10801 aaca
//
LOCUS       DAOSNB010000047        10789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6094_length_10789_cov_4.518446, whole genome shotgun sequence.
ACCESSION   DAOSNB010000047 DAOSNB010000000
VERSION     DAOSNB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10789
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_10789_cov_4.518446"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /gene="argS"
                     /locus_tag="PLG42_03430"
     CDS             complement(<1..553)
                     /gene="argS"
                     /locus_tag="PLG42_03430"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU30296.1"
                     /translation="MEYILRNKIAEAVRSLYNKDVDLNLIQIQKTRKEFNGDYTLIVF
                     PLIRCSKNTPEKTAEDIGNFLINNLPAISEFNVIKGFLNLKISDKWWADYFKDFCFLD
                     DIFLPCRVAKPEIILVEYSSPNTNKPLHLGHIRNNLLGYSLYKILKSCGHNVIKTNIV
                     NDRGIHICKSMLAWLKWGNGETPE"
     gene            781..1239
                     /locus_tag="PLG42_03435"
     CDS             781..1239
                     /locus_tag="PLG42_03435"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOU30297.1"
                     /translation="MKELKNLYKEPTSKTPEVDFDSLTGNLILSGRSIPENATDLYEP
                     LYNWIVEYAKNPRQVTNLRLNLEYFNTASSIWLAKIVKVLSHVDDPDKILYIHLYFNV
                     EEFDNMETEDIKDAISPVIDVLGGATISIGIKIYGTDEKGEIIKESMVLV"
     gene            1355..2818
                     /gene="amrB"
                     /locus_tag="PLG42_03440"
     CDS             1355..2818
                     /gene="amrB"
                     /locus_tag="PLG42_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOU30298.1"
                     /translation="MEMFSQSKTGDRQPAAAGRFYPASRETLLRDLEQMFASCDKYPE
                     KLKVRAIIVPHAGYIYSGRTAASAFSTIPADSKFKNIFLIGSSHIMAFEGASVYYTGD
                     FLTPLGKITVNKEIAEKLRKEHSVFRFQENAHSQEHSLEVQVPFIQYYFKNLQQIVPI
                     IIGTNNKNTIKTIAEALRPWFTPENLFIISSDFSHYPPYKEAKEVDNATADAIVSKDP
                     ELFLKTLSQNSSKRIPNLATSMCGWTSGLMLLYLVNNDPNLVFKKISYCNSGDSQYGN
                     KEEVVGYHAIVLAEVQKNKQPAGNQSQEMQFTQEEIDQMFKIARNSIATMLNEHRRLV
                     LNPSEILPGLKRNLGAFVTLRIDGKLRGCIGRFVSSEPLYEVINQMAIASAFNDTRFM
                     PLTKEEFKKIEIEISVLGPLKRINNISEIILGKHGIYIKKDFRSGTMLPQVATENKWT
                     VEEFLGYTSREKAGLGWDGWKDAELYVYEALVLEEKK"
     gene            2825..5257
                     /locus_tag="PLG42_03445"
     CDS             2825..5257
                     /locus_tag="PLG42_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30299.1"
                     /translation="MYHVISTGTAVFVLYLICHFFYKSGFLSLADHRKIWNLILAVTF
                     ITTASAGLILALKVTYKWENSFFDSLLRWHVETGIAFSFTGIIHLLWHLTYFKNLFTY
                     KKRLTEEIPVYKESYTSKHFAANLFFIGYLSSSVQLLLMREILNISGGYELITGTFLA
                     SWLISSAAGSYYAGKSLFNNLKRINTFFAISPILSLIMMILFHRLYINPGETPSFLTS
                     IIFNILVLFPFCFVSGFVFIKVLTAAKQNYIYNSGKSFSIETTGGIAAGLIVTLLTSG
                     ILNTYQILAISVIIFLLFLIMMYFKPDFNIRLISIAASIILILVSIWQGPDKFFRQIL
                     LKGINVESSSDTPYGNITKGIYELEESTYYNHRIIRWKNDEIEREENIHYAMLQHDNP
                     EKILLISGDPSSCINEILKYNVKKIYYVERDPELVRTFMTENFPGKEIMAIQNVDAYS
                     FIRKTHEKFDIVIMILPPPSTILLNRYYTKEFFENVKKKLNDGGIFACTPGSAENYYN
                     YQSASLYSVIYNTLASVFKNILPVAGNKLYFISSDNELSTNICSLLEKRGIKNIYVSS
                     DYLDDELIKMKSNEILSIIDYKTQINTIKRPIACFHFQSYNLTRDLGKIVPAFVIMLI
                     LFFLPVFTIKPVNFPMYSAAASLAGFEILILMSIQSSAGNIYHLTGLVIAAIMAGLSA
                     GAWIKPFFPEKKHVKYSMLILLTFYLITGVIINRIILMNSPAVAVAILLVISLIPSFI
                     TGSIFKVMTETESTNQTPAGVYSSDLAGSALGFIAISGVTVPLAGSLNTMLIISGLIF
                     IGFIFALMFQKY"
     gene            5278..6405
                     /gene="amrS"
                     /locus_tag="PLG42_03450"
     CDS             5278..6405
                     /gene="amrS"
                     /locus_tag="PLG42_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU30300.1"
                     /translation="MKGRKISKREFLKRCSVVSAGFVCFSPEMLRENPFSEQKTGLKL
                     AMYQEETPRGIMCRICPNECELKEGEVSKCNNRKVIKSKLYTMAYGNPCSVNIDPIEK
                     KPFYHFFPGTRAYSIATAGCNLACLNCQNWSISQTSPDKTRNYTLPPEKVVEECLNLK
                     CKSIAYTYSEPVSFYEYSYDTAVLAKAAGIKNVFKSNGYINREPLEKLCGVLDAANID
                     LKSFSESTYLRLSGGKLQPVLESLKIYRDNGVWLEITNLIVPTWTDKPEEIRNMCRWL
                     YKNGFASTPLHFSRFYPVYKLEQLPPTPLNILNQAAKIAAEEGIQYIYIGNVPGNEMA
                     DTRCPSCNTTVIKRTGYRIDFNKITDGKCPSCGKKIDGIWN"
     gene            6439..8445
                     /gene="ligA"
                     /locus_tag="PLG42_03455"
     CDS             6439..8445
                     /gene="ligA"
                     /locus_tag="PLG42_03455"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOU30301.1"
                     /translation="MKLSEAKKRIEELRKEINEHNRRYYVLNDPIISDFEYDLLMNEL
                     ETLEKMFPELITEDSPTRRVGSDLTKEFVQYRHKYPMLSLSNTYSAEEVMDFHNRVIK
                     VAGKDVEYVCELKFDGASISLTYVKGKLERALTRGDGYSGDDVTQNVLTIKSIPHELP
                     AGDYPEEFIIRGEILMSRPVFNELNKERAAEGLPLFANPRNAASGTLKLLDAKIVASR
                     KLECFLYYLLGENLPCDNHYGNMLKAREWGFRISEHMRLCKSIDEVMEYINHWDEARR
                     DLIFDTDGIVIKVNSLALQEELGSTAKSPRWAIAYKYKAEQVATRLLSVDFQVGRTGT
                     VTPVANLQPIHLGGTIVKRASLHNADQIELLDLHYNDTVFVEKGGEIIPKIVGVDTSK
                     REPGSKKVEFIKTCPECGTPLIRIENEANHFCPNYLHCPPQIKGRIEHFISRKAMNIE
                     GLGEETIDLLFNEGLVRNIADLYELKKEQLIPLERMGEKSAANIIASIEKSKNTPYHR
                     VLFALGIRHVGETIAKTLAAEFPDIDTLISADIEKLTSVREIGPKIAASIVNYFEDKD
                     NREIIARLKSYGINFRSEKETPEEISGKLAGKTIVISGVFSRHSREEYKEMIEKHGGK
                     NSSSISSSTSFVLAGENMGPSKREKAESLGIPIISEDEFLKMLE"
     gene            8438..9064
                     /locus_tag="PLG42_03460"
     CDS             8438..9064
                     /locus_tag="PLG42_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30302.1"
                     /translation="MNNIVEKNEILFAKNCYVCNMELFRKIRLKRGMSLLRKRAGDIK
                     RNKFKGNLADVKSIGIVWDATRTSDFQYLAQFHQKMQEKGVDVKIIGYYPDKILPDRI
                     TAIRYLSCLKKNDLNYFYLPTSEEAENFIKTRFDVLIDVNFNDLLPLQYITAMSVASL
                     KIGLFNEGNNSYLFDMMIDLNKNSKISDYFDHILHYLEMINNPAYIKN"
     gene            9069..9947
                     /gene="dapA"
                     /locus_tag="PLG42_03465"
     CDS             9069..9947
                     /gene="dapA"
                     /locus_tag="PLG42_03465"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOU30303.1"
                     /translation="MKKFRGTGVAIITPFKNDSSIDFSSLEKVINHVINGGVNYIVLM
                     GTTGESSTLTKDEKKALISFTLDTINKRVPLVVGIGGNNTQEIITQLKTTALKSVDGI
                     LSVAPYYNKPTQKGLYEHFKAIATCSPVPVIMYNIPGRTGCNISAETCLQLAHEFDTI
                     VGVKEASGNMEQIMKIIKGKPENFQVVSGDDLLTLPIIAAGGTGVISVIANAYPAEWS
                     EMVNQCLKSNFKAAREIMFRFTEIIELLFADGNPAGIKAAMNILNLCQNTLRLPLVPV
                     SQQVHARLKKAMESIQ"
     gene            complement(9948..>10789)
                     /locus_tag="PLG42_03470"
     CDS             complement(9948..>10789)
                     /locus_tag="PLG42_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOU30304.1"
                     /translation="IANMMYDVTESIVYFYHETGNFEEFEFDLIRSFSMDSPVPRDKF
                     LKLSKEEIVDIVYKKVLEVYKRKEEIIAGQAYPVLKDVYDRMSHLYENVVVPVSDGVR
                     VFQVIANLKASVESKGREIMRAFEKTVVLATIDEAWKEHLREMDDLKQAVQNATYEQK
                     DPLLIYKFESYELFKSMLNKINRDVVSSLIKGFIPVQDAEQVREAERRRKVDTSRLRE
                     EKSDFSQYSSTVPGQVGQKKVEPVRVEKKVGRNDPCPCGSGKKYKNCHGRMESPVTPP
                     VNR"
BASE COUNT         3553 a         1961 c         2067 g         3208 t
ORIGIN      
        1 tttcgggtgt ttcaccgttc ccccatttga gccacgcgag cattgatttg catatatgaa
       61 taccccggtc atttacaata ttagttttga taacattatg accgcatgat ttcagtattt
      121 tgtaaagaga atatccaaga agattatttc gtatatgccc cagatggaga ggtttattgg
      181 tattggggga agagtattcc accagtataa tttcgggttt tgcaaccctg cagggtaaaa
      241 agatgtcatc caggaaacag aaatctttga aataatcagc ccaccattta tcggaaatct
      301 tcaaattaag aaatcctttt attacattga attcagaaat agccggcaaa ttgttaataa
      361 ggaaattacc aatatcttca gcagtcttct caggggtatt tttcgaacat cgtattaatg
      421 gaaaaacaat aagagtataa tcaccattaa attcttttct ggttttctga atttgaatca
      481 gatttaagtc aacatccttg ttatagagtg accttacagc ttccgctatt ttgttcctga
      541 gaatatattc cataaaattt gtaaattcag cggcaaatat aattctttta tgcttttgcg
      601 ggaaagtaaa aataagtttg ggccatttaa accttaatct ttacccattt taggtgaaat
      661 gcataaaata tttgacaatt gtattattta ttttgataaa ggttttatct gactatataa
      721 aataacttta tatttggatt gtaataatta aaaatttacc ggttttttat aatccaagta
      781 atgaaagaat taaaaaatct ttacaaagag ccaacttcaa aaactcccga agtagatttt
      841 gactcattaa caggcaatct tatcctgtca ggaaggtcca tacctgagaa tgcaaccgat
      901 ttatatgaac cactatacaa ctggatagtg gagtatgcaa agaatcccag acaggtgaca
      961 aatttaaggc tcaatcttga gtattttaac acagcctctt cgatatggct ggccaaaatt
     1021 gtaaaggttt tgagtcatgt ggatgatcct gataaaatac tctatattca tctctatttc
     1081 aatgtggaag aatttgacaa tatggagact gaagacatta aggatgcaat cagtccggta
     1141 attgatgtcc ttggcggggc tacaatcagc ataggaataa aaatatacgg taccgacgaa
     1201 aaaggggaaa tcataaaaga gtcaatggtt ctagtttaga acaatctttt tatctcaagg
     1261 atttttcatt tttttctagt aattttaata ccaaatttaa ccgcgcagcg atatgcgatt
     1321 tttgtgtatt atattaatat caacactatt tacaatggaa atgttttccc agagtaaaac
     1381 cggagatcgt caacctgctg cagcaggaag gttttatccg gcaagccgtg aaactttgct
     1441 tcgcgatctc gagcaaatgt ttgcatcatg tgataagtat ccagaaaaat taaaggtaag
     1501 agcaattatt gtcccccacg ctggttatat ttactcaggt agaacggcag cttcagcatt
     1561 ttcgacaata ccagcagata gtaaattcaa aaacattttt cttatcggct caagccatat
     1621 tatggcattt gaaggcgcct cggtttatta tacgggagat tttttaactc ctcttggaaa
     1681 gattacagta aataaggaaa ttgcagaaaa acttcgaaaa gagcacagcg ttttcaggtt
     1741 tcaggaaaat gctcattctc aggaacatag ccttgaggtt caagttccat ttatccaata
     1801 ttatttcaag aaccttcagc aaattgtgcc aataattatt ggaacaaata acaaaaatac
     1861 cattaaaact attgccgaag ctttaagacc atggtttact ccggaaaacc ttttcattat
     1921 cagcagtgat ttctcccact acccaccata taaagaagca aaagaagtgg ataatgccac
     1981 agctgatgcg atagtatcaa aagatcctga attgttttta aagactctct cacaaaactc
     2041 atcaaaaaga attccgaacc ttgctacaag tatgtgtggc tggacttcgg ggttgatgct
     2101 tctctacctt gtgaacaatg acccgaacct tgtattcaaa aaaataagtt actgtaattc
     2161 tggcgattca cagtatggca ataaagaaga agttgtgggt taccacgcaa tagtccttgc
     2221 agaggttcag aaaaataaac aaccagccgg taatcagtcg caggaaatgc agtttacaca
     2281 ggaagaaatt gaccagatgt tcaagattgc cagaaattca attgccacaa tgcttaatga
     2341 acaccgtaga cttgtattga atccttcaga gattcttccg ggactgaaga gaaacctggg
     2401 agcttttgtt actctcagaa ttgatggaaa actaagagga tgtattggca gatttgtttc
     2461 gtcagagccc ctatacgaag tcataaacca gatggcaatt gcatcggctt tcaacgacac
     2521 ccgttttatg ccactgacaa aggaagaatt caagaaaatt gaaattgaaa tatctgtgct
     2581 tggtcctctt aaaaggatta acaacatttc cgagattata ctcggaaaac acggcattta
     2641 catcaaaaaa gacttcagat caggtaccat gcttccgcag gtggctacag agaataaatg
     2701 gacagttgag gagtttcttg gatatacctc ccgtgaaaaa gctggtcttg gatgggatgg
     2761 atggaaagat gcagaattat acgtttatga agcactggtg cttgaagaaa agaaataaca
     2821 gataatgtat catgtaataa gtacagggac tgcagttttt gtattgtatc taatatgtca
     2881 tttcttttat aagtcaggtt ttctctccct tgccgaccac cggaaaatat ggaatctaat
     2941 attagcggta acatttatca caaccgcatc agcaggtctc atcctggctc tgaaagtaac
     3001 atacaaatgg gaaaacagtt tctttgactc cctgctcaga tggcatgttg agacaggtat
     3061 agcgttctct tttacaggga taattcattt gctgtggcac ctcacatatt tcaaaaacct
     3121 gttcacttac aaaaagagac taactgaaga gattcctgtt tacaaagagt catatacttc
     3181 aaaacatttt gccgccaatc tgttctttat aggatatctg agctcttcag tacagttatt
     3241 gctgatgaga gaaatactta acatttcagg tggatatgaa ttgataacag ggacctttct
     3301 cgcatcatgg cttataagtt ctgcagcagg ctcatattat gcaggaaaat cattatttaa
     3361 taacctgaaa agaataaata ctttttttgc tatcagccct attctctctc ttataatgat
     3421 gatccttttc cacagattat atatcaaccc tggtgaaaca ccttcctttc ttacaagcat
     3481 aatatttaac atccttgtgc ttttcccgtt ctgctttgtt tctggatttg tatttattaa
     3541 agtattgacg gccgcaaaac aaaattatat ttataatagt ggtaaatcat tttcaataga
     3601 gacaacagga ggaattgcgg caggacttat tgttaccctg ctaacatcag gcatcctaaa
     3661 tacatatcag atcctggcaa tttctgtaat aatattcctg ctgtttctca taatgatgta
     3721 tttcaaacct gattttaata tcagactaat ttcaatagcg gcaagtatta tattaatact
     3781 tgttagcatc tggcagggtc cagacaagtt tttcagacag attcttctaa aaggaataaa
     3841 tgtagaatct tcttccgaca ccccttacgg caacataaca aaaggaatat atgagttaga
     3901 ggaatcaaca tactataatc accgtattat cagatggaaa aacgatgaaa ttgaacggga
     3961 ggaaaacata cattatgcga tgcttcaaca tgataaccca gaaaaaatac tcctaatttc
     4021 aggcgatcca tcctcatgta taaatgagat tctgaaatat aatgtgaaaa aaatatatta
     4081 cgttgaaaga gatcctgaac tggtaagaac ctttatgact gaaaattttc ccggcaaaga
     4141 aataatggct atacaaaatg ttgatgcata cagttttatc agaaagaccc acgaaaaatt
     4201 cgatattgta attatgatcc tccctccccc atcaacaatc cttcttaacc ggtactatac
     4261 aaaagaattt tttgaaaatg ttaagaaaaa gcttaacgat gggggaatat tcgcatgcac
     4321 acccgggtca gctgaaaatt actataacta ccagtcggca tcattgtatt cggtaattta
     4381 taatactctg gcatctgtat ttaagaatat ccttcctgtt gcaggtaata aattatattt
     4441 tatatcgtct gataatgagc tgagtacaaa tatttgctct ctcctcgaaa aaaggggaat
     4501 aaaaaatatt tatgtaagct ccgattatct ggatgatgaa ttaattaaaa tgaaatccaa
     4561 tgagatcctc tccataattg attataagac acaaattaac actattaaac gtcctattgc
     4621 atgttttcac tttcagtcat ataatctgac tcgagacctg gggaaaattg ttcctgcatt
     4681 tgttattatg cttatccttt tcttccttcc tgtatttaca ataaaacccg tgaattttcc
     4741 gatgtactct gctgcagcat cattagctgg atttgaaata ttaatcctga tgtccattca
     4801 gtcatcggca ggtaatattt accatttgac cggacttgta attgctgcaa taatggccgg
     4861 actttctgca ggtgcatgga taaaaccttt tttcccggag aaaaaacatg taaaatattc
     4921 catgttaata ctattgactt tttatttaat tactggagtt ataataaaca ggataatttt
     4981 aatgaacagt cctgctgtag cagttgcaat tttactagta ataagtttga taccttcgtt
     5041 tattacaggt tccatcttta aggtcatgac tgaaacagag agtactaatc aaacacctgc
     5101 aggtgtgtac agttccgatc tggcaggttc tgccctcgga tttatagcaa tatctggggt
     5161 tacggttcct cttgcaggtt ccctaaatac aatgctgata atatccggtt taatcttcat
     5221 aggatttata tttgcattaa tgtttcagaa atattaagta aaaaacaggg agggaatatg
     5281 aaagggagga aaataagcaa gcgggagttt ttaaaaagat gctcagttgt ttcggctggt
     5341 tttgtttgtt tttcaccgga aatgctcagg gaaaatccat tttctgaaca gaaaaccgga
     5401 ttaaaactgg caatgtatca ggaagaaaca ccaagaggta taatgtgcag gatatgtcca
     5461 aatgaatgtg agttgaagga aggggaagta agcaaatgca ataatcggaa agttatcaaa
     5521 tcgaaacttt atacgatggc ttacggcaat ccctgttcgg taaatatcga ccctatcgag
     5581 aaaaaaccat tttatcactt tttccctggt acaagggcat attccattgc aacagctggt
     5641 tgcaaccttg catgcttaaa ctgtcagaac tggtctatat cccagaccag tccggataag
     5701 acaaggaact atacgttacc acctgaaaag gttgttgagg agtgtctgaa tctgaaatgc
     5761 aagtctattg cttacactta ttccgagccg gtttcctttt atgaatattc gtacgataca
     5821 gcagtactgg caaaagcagc aggtattaaa aatgttttca agtcgaatgg ttacataaac
     5881 agagaacctc ttgaaaaact ctgcggagtg ctggatgctg cgaatattga tcttaaatct
     5941 ttcagtgaaa gcacatatct aaggttatcg ggcggtaaac ttcagcccgt tcttgaatcg
     6001 cttaaaatat accgtgacaa tggtgtatgg cttgaaatta caaaccttat tgttccaacc
     6061 tggactgaca agcctgagga aataagaaat atgtgccgct ggctttacaa gaacggtttt
     6121 gcttcaacac cattacactt cagcagattt tatccggtct acaagctcga acagttacct
     6181 cctactccgc tgaacattct gaatcaggct gcaaaaatag ctgccgaaga aggaatacaa
     6241 tatatttata ttggcaacgt accggggaac gaaatggctg ataccagatg tccttcgtgc
     6301 aacactactg taattaaaag gacaggatac cggatagatt tcaacaagat aactgatggc
     6361 aaatgtccat cgtgtggcaa aaagatagat ggaatatgga attagattta atttattaaa
     6421 atactcttac ccgaaattat gaagctgagt gaagcaaaga aacgtattga agaacttcga
     6481 aaggaaataa atgaacataa cagacggtat tatgtattga acgatcctat catttctgat
     6541 tttgaatatg atttactgat gaatgagctg gaaactcttg aaaagatgtt tccggaactg
     6601 ataactgaag attcacccac aaggagagtt ggcagtgatc tgacaaaaga gtttgtacag
     6661 taccggcata aatacccgat gttatcgctc agcaacactt attcggcaga ggaagttatg
     6721 gattttcata acagggttat aaaagttgca ggcaaagatg ttgaatacgt atgtgagctg
     6781 aaatttgacg gggcatctat cagtcttact tatgtaaagg gaaaacttga gcgtgcactt
     6841 acgaggggag atgggtattc aggcgatgat gtgacccaga atgtacttac aataaaatca
     6901 attccgcatg aattaccggc aggagattat cctgaggaat ttatcatcag gggggaaatc
     6961 ctgatgtcac ggccagtatt taatgaactt aacaaagagc gtgcagctga gggactaccg
     7021 ctttttgcca atccgagaaa tgcggcttca ggaaccctga aacttcttga cgctaaaata
     7081 gttgcaagta gaaaactgga gtgtttcctt tactaccttc tcggggaaaa tttgccctgt
     7141 gataaccatt atggcaatat gttaaaagcc agggaatggg gattcaggat ctcagaacac
     7201 atgaggttat gcaaaagcat tgatgaggtt atggaatata taaatcactg ggatgaggca
     7261 agaagggatt tgatatttga tactgacggt atagtaatta aagtaaattc tctggctctt
     7321 caggaagaac taggttccac tgctaagtcg ccacgttggg caatagctta caaatacaag
     7381 gctgagcagg ttgccacgag actcctgtca gttgatttcc aggttggaag gaccggaaca
     7441 gtaacacctg tagctaatct tcagccaatt catctcggag gaactatagt aaaaagggct
     7501 tcccttcaca atgcagatca gattgagtta cttgatcttc attataatga taccgttttt
     7561 gttgaaaaag gaggtgaaat aattcccaaa attgttggtg ttgacacaag caagagggag
     7621 ccaggaagta aaaaagtgga atttataaaa acctgccctg aatgcggtac accgctaatc
     7681 aggatagaga atgaagcaaa tcatttctgt ccaaattatc tccattgtcc acctcagatt
     7741 aagggcagaa tagagcattt tatttcacga aaggcgatga atatagaagg gctgggagaa
     7801 gagacaatag accttctttt caatgaaggt cttgttagaa acatagcaga cctatacgaa
     7861 ctgaaaaagg agcagctgat acctcttgaa aggatggggg aaaaatctgc agcaaacatt
     7921 attgcaagca ttgaaaaatc aaagaatact ccttatcata gagttttatt tgcccttgga
     7981 atacgccatg taggcgaaac tatagcaaaa actctggcag ctgaatttcc cgatattgat
     8041 accttgatat cggcagatat tgaaaaactg acatctgtca gagaaatagg ccctaaaata
     8101 gctgccagca ttgttaatta ttttgaagat aaagataaca gagaaataat tgccaggctc
     8161 aaatcgtacg gaatcaattt ccgttcagaa aaagagacac ctgaagagat ctcaggcaag
     8221 ctagcaggta agacaattgt gatttcagga gtcttttcca ggcattccag agaagaatat
     8281 aaagaaatga ttgaaaagca cggaggtaaa aattcatcgt ctatttccag cagcacttca
     8341 tttgtacttg caggtgagaa tatggggccc agtaaaagag aaaaagctga aagccttggc
     8401 ataccaataa taagcgaaga tgaatttctg aaaatgcttg aataatattg tcgaaaagaa
     8461 tgaaatactt tttgcaaaaa actgttacgt ttgcaacatg gaattgttca gaaaaataag
     8521 acttaaaaga ggaatgtcgt tgctcagaaa aagagcagga gatataaaac ggaataaatt
     8581 caaaggtaat ctggctgatg ttaaatcaat cggtattgtc tgggatgcca cacgaacatc
     8641 tgattttcaa tatcttgcac aatttcatca gaagatgcag gaaaaaggag tggatgtaaa
     8701 aataataggg tattatcccg ataaaattct tcccgaccgc ataacagcca tcagatatct
     8761 gtcctgtttg aaaaaaaatg atcttaatta tttttatttg cccacatcag aggaagcaga
     8821 aaattttata aaaacccggt ttgatgttct gatagatgta aatttcaatg atttacttcc
     8881 acttcaatat atcactgcca tgtcagtggc gtcattaaaa attgggttat ttaatgaagg
     8941 gaataattca tatctgtttg atatgatgat tgatctcaac aagaattcaa aaataagcga
     9001 ttattttgac catatattac attatcttga aatgattaat aatccggcat atataaaaaa
     9061 ctgatcatat gaaaaaattc agaggaacag gggtggcaat aataactcct tttaaaaacg
     9121 attcgagcat tgatttttca tcccttgaaa aagtaataaa tcatgttatc aacggtggag
     9181 tcaattatat tgttttaatg ggaacgacag gagaatcgtc cactctcaca aaagatgaaa
     9241 agaaagccct gatttcattt actctggaca caatcaataa aagagttcct ctggttgtag
     9301 gtatcggagg aaataataca caggagatta ttacccagct gaaaacaact gccctgaaat
     9361 cagttgacgg gattctctct gtagctccat attataacaa accgactcaa aaaggactat
     9421 atgagcattt taaagcaatt gccacctgca gccctgttcc tgtaattatg tataatatac
     9481 caggccgtac tggatgcaac atctcggcag aaacatgttt acagcttgcc catgaatttg
     9541 atacaattgt tggtgtaaaa gaagcttcgg gcaacatgga acaaattatg aagataatta
     9601 aaggaaaacc cgaaaatttt caggttgtat ccggagatga ccttttgact ctgccgataa
     9661 ttgctgcggg gggtacgggt gttatatctg taattgcaaa tgcatatcct gccgaatgga
     9721 gtgagatggt caatcagtgt ctcaaatcga atttcaaagc agcgcgtgaa attatgttcc
     9781 gtttcactga aataattgaa ttgctgtttg ctgatggcaa tcctgcaggg ataaaagctg
     9841 ctatgaatat tctgaatcta tgtcagaaca ctctcagatt acctcttgtt ccagttagcc
     9901 agcaggtcca tgcaagactg aagaaggcga tggagtcgat tcaataacta cctgttcacc
     9961 ggtggtgtta ccggtgattc cattctgcca tgacaatttt tatatttttt accgcttccg
    10021 cagggacacg ggtcattgcg tccgactttt ttctcaactc ttacaggttc cacttttttc
    10081 tggcctacct gacctggaac agtgctgctg tattgggaaa aatcgctttt ttcttctctt
    10141 aagcggcttg tgtcaacttt ccggcgtcgt tcagcttctc taacctgttc tgcatcctgt
    10201 accggtataa agcctttaat cagagagcta acaacatcac ggttaatctt gttaagcatt
    10261 gatttgaaca gttcatacga ttcaaacttg taaattaaca ggggatcctt ctgttcataa
    10321 gtagcgttct gtacagcctg tttaaggtcg tccatctccc tgagatgctc cttccatgct
    10381 tcatctattg tagctaatac tactgttttt tcgaaagctc tcataatttc cctgcccttg
    10441 ctttcaacac ttgccttcag gtttgcaatt acctgaaaaa cccttacccc atcacttaca
    10501 ggcacaacaa cattttcgta cagatgtgac attctgtcgt aaacatcttt caacacagga
    10561 tatgcctgac ctgcaataat ttcttccttt cttttgtata cttcaagtac ttttttatag
    10621 actatatcaa caatttcttc tttggaaagc ttcaggaact tatctcttgg cacaggagaa
    10681 tccattgaaa atgaacgaat aagatcaaac tcaaattctt caaaattgcc agtttcgtgg
    10741 tagaaataaa ctatactttc tgtgacatca tacatcatat tggcaatat
//
LOCUS       DAOSNB010000048        10684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6176_length_10684_cov_3.719823, whole genome shotgun sequence.
ACCESSION   DAOSNB010000048 DAOSNB010000000
VERSION     DAOSNB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_10684_cov_3.719823"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PLG42_03475"
     CDS             <1..1419
                     /locus_tag="PLG42_03475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOU30305.1"
                     /translation="DFSSGTSSRSTKLVHGGVRYLAQMDILLVIEALRERGIMLRNAP
                     HLTGNQEFIIPVYSWFDAMFYTIGLKFYDVLSGKLSLGKSYFIGRKKVIERLPNIKQE
                     GLKGGIVYHDGQFDDSRMAVALAKATTKHGGTVLNYFEVTALLKDEKGKIKGVKATDT
                     ISGRKYEIFSSLVINATGVFADNILRMDRPDAHRIIKPSQGIHLVLDGSFLQGDSALM
                     IPKTEDGRVLFAIPWYGKTVTGTTDTPLEKISEEPVALKEEIDFVLRTAGNYMVKAPA
                     KKDILSVYAGLRPLASDPDNPHATKEISRRHKIIVSSSGLITVTGGKWTTYRCMAEET
                     IDRAISEGLLEKRKCVTRTLKVEALPEKIANNRLRIYGENAGEIENIMAENPDTAEKI
                     SDKLPYTKAEIIWICRNEMPVTLEDMLARRTRALIFDARAAEKCAPLVAEIMAGEMGH
                     DGRWIEEQVASFRKLVKKYLPE"
     gene            1436..2932
                     /gene="glpK"
                     /locus_tag="PLG42_03480"
     CDS             1436..2932
                     /gene="glpK"
                     /locus_tag="PLG42_03480"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOU30306.1"
                     /translation="MKKYILALDQGTTSSRAIIFGHDGLPVAIAQKEFTQLYPKPGWV
                     EHNPVEIWTSQSDVAIEAMVKAGIKASDVAAIGITNQRETTIVWDRTTGKPIYNAIVW
                     QDRRTAEYCDRLKAEGNAEMIRQKTGLVIDAYFSATKVKWILDNVDGARRLASEGKLA
                     FGNVDTWLVWNLTGGKLHITDVSNASRTMLFNINTLEWDSDLLRLFDIPESVLPEVKP
                     SGEVYGEAVVPLGEPVVISGIAGDQQAALFGQMCVEPGMVKNTYGTGCFMMMNIGDRP
                     VMSKNMLLTTIGWKIGNVIQYALEGSIFIAGAVVQWLRDGLGIIKTSAEVEKLAGSVS
                     SSDGVYFVPAFAGLGAPHWEQHARGTITGITRGTTSAHIARAALDSIAYQTLEVLKAM
                     EKDSGIKISELRVDGGATVNNLLMQFQADILQAKVVRPVITETTALGAAYLAGLATGF
                     WSGTEELKKQWQIDHAFLPRIDSETAARLTAGWERAVNSVKYWAGLQG"
     gene            3192..3908
                     /locus_tag="PLG42_03485"
     CDS             3192..3908
                     /locus_tag="PLG42_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOU30307.1"
                     /translation="MNQFFAEFFGTAMIIVFGSGVVSNVLLKNTKGHNSGWIVISFGW
                     AVGVFTGVLIAAGTSGAHLNPAVTLALVIAKKFSISMVPIYISAQLLGAMFGAGLAWL
                     AYKKHFDATDDGDAKLAVFCTGPNIRSYGYNLITEIIGTYVLALAVLYMAQPEVGLGA
                     LNALPVAIVVLGIGLSLGGPTGYAINPARDLGPRIMHFLLPIPKKRDSDWRYSWVPIV
                     GPLIGASLAAIMYIIFEKGI"
     gene            4224..5429
                     /locus_tag="PLG42_03490"
     CDS             4224..5429
                     /locus_tag="PLG42_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU30308.1"
                     /translation="MKKIIFFLIVAVNCWTALAQVPGLQGGQQPAPLPEGFKPSECNT
                     FLSQYPAVNAQTREALFRVVAPGARIVQVDLAGKRYNMVKDEKGVWTCKSDPQVVGFH
                     YYSIWIDSVPVMDRNTKAFFGSNWESSGIEIPEGPEGDYYRFNKNIPHGHIRSMYYWS
                     DINGLERHCNVYVPAEYENNPQKRYPVLYLLHGWGEDEDGWVNQGHIANIMDGLIASG
                     KAVPMIVVMDCGDFKTNPDVRRASTNDITQIYVKELIPFIDKTFRTKTDRLNRAMAGL
                     SRGGMQTTMTVFNNPDKFAWLGTFSGFFMGGFGNRTAAGGGQAPGMTQQIQTAFNGIF
                     KDPEAFNKQFKLLFISTGTEERSPKEQVEALKAYGIKNIVFHESQGTAHEWLTWRRAL
                     YDFAPRIFK"
     gene            5472..8129
                     /locus_tag="PLG42_03495"
     CDS             5472..8129
                     /locus_tag="PLG42_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30309.1"
                     /translation="MNEKIKAGNHCIALIITFLALFVTVSCYRRAEFTINDRRICFDE
                     EWRFIRDSLAGAENPEYDDSSWRLLDLPHDWSIEDLPGQNGVDIIGPFDKNAVDRMAS
                     GYTVGGTGWYRKKFKTTEEYRNKRVFLEFDGVYMNADVWLNGNHLGFHPYGYTPFHFD
                     ITPYLNEPGKENVVAVRVRNEGLNSRWYSGSGIYRHVWIIPVDPVHIDIAGGIFVTTP
                     EVNPNSAQVDVAVTLVNDGQNEKSITVQSELFDKKGKSAGKISSSDVTLTPGITKVIH
                     KLNVKKPLLWSLDEPNLYRARVSVMSVDGEVIDECEAPFGIRIIKIDAVKGLTVNGKT
                     VKMIGGCFHHDNGPLGAVAIDRAEERKIAILKKTGFNAIRTSHNPPSTELLNACDRIG
                     MLVIDEIFDMWETPKREKDYHQYFKEWWQRDVESWVKRDRNHPSVVIWSIGNEIRETY
                     DTTGLRIARNLTGEIRRLDPTRAVTEAFNDFGWRRGQGSRWHEIPEHMALLDVIGYNY
                     MYQRYEEDHERYPDRVMVGTETNPPLALENYEIMMKHPYVIGYFVWTAIDNIGEAGVG
                     MPQLRDAGQEVQVQGAPASGPGAGFQQGVQVGATGRAPGGVPAGTQQAAQGTPGIPGA
                     PQGMPGGGFFRRETWPVFTNYQGDLDLTLNRKVPSYYQYVVWGKSKVEMFVHRPIPEG
                     KVEVTSRWGFPDEVKSWNWAGREGQKFQVHVYTRSQKVRLELNGKIVGEQEVDQTKSI
                     TATFEVPYEPGTLVARCFDNGRETASERLKTTGRPAAIRLTADRTVIRAGKNDLSYVT
                     AEIVDSNGFVVPDADSIMVNFEVTGNGKVAGVGSGDPRDMSSFQQPRKKTYEGRCIAV
                     IKPGREPGKIRVQATAEGLRGASVVITVK"
     gene            complement(8389..8460)
                     /locus_tag="PLG42_03500"
     tRNA            complement(8389..8460)
                     /locus_tag="PLG42_03500"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8426..8428),aa:Met,seq:cat)
     gene            8833..9216
                     /locus_tag="PLG42_03505"
     CDS             8833..9216
                     /locus_tag="PLG42_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOU30310.1"
                     /translation="MPRVFKATDIENEAKESRRDYFDRHTPHIICPDKVKKGEKFEVK
                     VKLGENYVHPDDPDHYISLIQLWNRETLLAEVRYTAGAYGNKPGHFEVGFYIVAPEVN
                     MNLSAMSVCTKHGLWQSDDKPVEVI"
     gene            9307..10179
                     /locus_tag="PLG42_03510"
     CDS             9307..10179
                     /locus_tag="PLG42_03510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU30311.1"
                     /translation="MAKEEKEKKEKKKNSWREKYRFTIYNDTTFEEVWKIRLTRYNAF
                     LVVSFIVLFIIGSTVSLIAFTNLREFIPGYPDSEMRRNILMNAIRLDSLERELDLRDK
                     YFANLNAIISGKQPVDILATPDTGKKYSNIQFSSIPEVETLKTEIEKEERFNLTLPRL
                     TEPAGLAGLHFFPPVKGIITAKFDPRAKHFGVDIVTRPKSPVMAALDGTVVFTGWTME
                     TGYVIQIQHSNNLITVYKHNASLLKENGDYVRAGEAISVVGSSGEMYTSGPHLHFEIW
                     YKGIPLDPEKHVLF"
     gene            10203..>10684
                     /locus_tag="PLG42_03515"
     CDS             10203..>10684
                     /locus_tag="PLG42_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU30312.1"
                     /translation="MLKPKRIAILGSTGSIGTQVLDIISRYPGRFRPEVLTCKSSADL
                     LIEQARRYKPARVVIANKSLYARVKDGLKDLPVEVMAGNEELEYAATLDEIDMVVAAM
                     VGYAGMKPVLAAVKAGKNIALANKETLVVAGELIGELTASYHASILPVDSEHSAIFQC
                     "
BASE COUNT         3179 a         2030 c         2620 g         2855 t
ORIGIN      
        1 gatttttctt ctggaacctc ctcaagaagc acaaaacttg tacacggtgg agtcaggtat
       61 ctggcccaga tggacatcct tctggttata gaggccctga gagaacgcgg tattatgctc
      121 aggaatgcac ctcatctaac aggtaatcag gaatttatta taccagtata ttcctggttc
      181 gatgctatgt tttatactat cgggctgaag ttttatgatg ttctttctgg aaaattgagt
      241 ctgggaaaat catattttat cggacgtaag aaagtgattg aacgattgcc taacattaaa
      301 caggaggggc tcaaaggagg tatagtttac cacgacggtc agtttgatga ttcaagaatg
      361 gccgttgcac tggcaaaagc aaccacaaag catggtggta cagtgcttaa ttattttgaa
      421 gttacagctc ttctgaaaga tgagaaaggt aagataaagg gtgttaaagc cacagacaca
      481 atttcgggcc gaaagtatga gatattttct tctctggtta ttaatgctac tggtgttttt
      541 gccgacaata tattgcggat ggaccgccct gatgctcacc gcataataaa gccgagtcag
      601 ggaattcacc ttgtacttga tggctctttt cttcagggag attccgcact tatgatacca
      661 aagactgaag atggccgcgt gctatttgcc ataccatggt acggaaaaac agttactgga
      721 acaacggata cacctcttga gaagataagc gaagagcctg ttgcacttaa agaagaaatt
      781 gattttgttc ttcgtacagc aggcaattac atggtaaaag ctcctgcaaa gaaggatatt
      841 ctgtcggttt atgccggact caggcctctg gcttcagatc ctgataaccc tcatgcgaca
      901 aaggaaatat caagacgtca taagataata gtatcatctt cgggattgat aactgttacc
      961 ggcgggaaat ggaccacata tcgctgcatg gcagaggaaa ccattgaccg tgctatttct
     1021 gaagggttgc tcgagaaaag aaaatgtgtc accagaacac ttaaagttga agctttaccc
     1081 gaaaaaatag cgaataacag attgaggatt tatggagaaa atgccggtga aattgaaaac
     1141 attatggcag aaaacccgga cactgctgaa aaaatctctg ataaactacc ttatactaaa
     1201 gctgaaataa tctggatttg cagaaatgag atgcctgtta ctcttgaaga catgcttgca
     1261 cgcaggacaa gggccctgat ctttgatgct agagctgctg aaaagtgtgc cccgcttgtg
     1321 gctgagatta tggcaggtga gatgggacat gacggcagat ggatagagga acaggtggca
     1381 agtttcagaa aactggtgaa aaaatattta cctgaataat gaaaattaat cccctatgaa
     1441 aaaatacatt cttgcacttg accagggcac aacaagctca agagcaatca tttttggtca
     1501 tgacggatta cctgttgcaa tagcacagaa ggaatttaca cagctctacc cgaaacccgg
     1561 gtgggtagag cataatccgg ttgagatctg gacatcgcag tcggatgttg ctattgaggc
     1621 aatggttaaa gctggaataa aagcatcaga tgttgcagca atcggaataa caaaccagcg
     1681 tgaaacaacc attgtatggg acagaacaac cggaaagcca atttataatg caatagtttg
     1741 gcaggacagg cgtactgctg aatactgcga cagactgaaa gcggaaggaa atgctgaaat
     1801 gattcgtcag aaaacgggac ttgtaataga tgcatacttt tcggcaacaa aggtaaaatg
     1861 gatactggat aatgttgacg gtgcacgaag gctggcatcg gaagggaagc tggcatttgg
     1921 caatgtcgat acatggcttg tatggaatct cacgggcggg aaactacata ttactgatgt
     1981 ttcaaatgca tcacgaacaa tgctttttaa cataaataca cttgagtggg atagtgatct
     2041 gctcaggctg tttgatattc ctgaaagtgt tcttcctgaa gtgaaaccgt cgggtgaagt
     2101 atatggtgaa gctgttgtgc cattaggaga accagtagtc atttctggca tagcaggcga
     2161 tcagcaagct gcactcttcg gacagatgtg tgttgaaccg ggtatggtca aaaacacata
     2221 cggaacagga tgttttatga tgatgaatat tggcgacagg ccggttatgt cgaaaaacat
     2281 gctgttaacg actataggtt ggaaaatcgg taatgttatt cagtatgcac ttgaaggaag
     2341 tattttcatt gccggagctg ttgttcaatg gctgcgcgat ggacttggta taattaaaac
     2401 ttcagctgag gttgaaaagc ttgccggcag tgttagctca agcgatggag tatactttgt
     2461 tcctgcattc gccggcctgg gagcacctca ctgggagcag catgccaggg gtacaataac
     2521 cggcataacc cgtggaacaa cttcggcaca tattgcccgt gccgcactcg acagcatagc
     2581 ataccagaca cttgaagtgc ttaaagctat ggagaaagat tcgggcataa aaataagcga
     2641 gttgagggtc gacggcggag ccactgtaaa taaccttctg atgcagtttc aggcagatat
     2701 tcttcaggct aaggttgtaa ggcctgtcat taccgaaaca actgctcttg gagctgcata
     2761 tctggctggt ctggcaacag gcttctggag cggaacagaa gaacttaaaa agcagtggca
     2821 gatagaccac gcatttttac cacgtataga ttcggaaaca gctgccaggt tgactgcggg
     2881 atgggaacgc gcagtaaata gcgttaaata ctgggcaggt ttgcagggtt gagattatag
     2941 cgcccataaa taaaattatg aattattttt cttttcagag ctctctaaaa agatgtttat
     3001 taagtgagtg tattaaaaat atagaagcta aaatattgaa tatcaataaa tcttctctca
     3061 gggcctgttc aattttgcgg ttgtaaaggt ggggattcag aggtgaaaaa aaaggctttt
     3121 tagagagctc ctggagacaa gaaaatatga aaacaaaaat taaaatttaa ttttcaataa
     3181 atcttgaaag catgaaccag ttttttgcag aattcttcgg aacagcaatg attatcgttt
     3241 tcgggagtgg cgtggtatca aacgtacttc tgaaaaacac gaaaggacac aacagcggct
     3301 ggatagttat atcttttggg tgggcagtgg gtgtatttac cggtgtattg attgccgctg
     3361 gtaccagcgg agcccatcta aatccagctg ttacacttgc acttgtcata gcgaaaaagt
     3421 tttccatctc tatggtgccc atttatattt ctgcgcaact tctgggcgcc atgtttggag
     3481 caggccttgc atggctggca tataaaaagc atttcgatgc taccgacgat ggcgatgcga
     3541 agctggcagt gttctgcacc ggtccaaaca taaggagcta tggttataat ctgataacag
     3601 agattatagg tacctatgtt ctggcacttg cggttcttta tatggcacaa cctgaagtgg
     3661 gtctaggggc tttgaatgct ttaccagtag caatagtagt gctgggaata ggtctttctc
     3721 ttggtggccc tacaggttat gccatcaatc ctgcacgtga ccttggtcca agaataatgc
     3781 attttcttct tcctatacct aaaaaacgcg acagcgactg gcggtattca tgggtgccga
     3841 tagtgggacc acttatcgga gcctcattag ccgccattat gtatattatt tttgagaagg
     3901 gcatataacc tgtcaggttt ttgccagatt ctattttttc ttgaacttct ttttactgca
     3961 cttgtaacgt tgtgcaggtt cagctgtttt tccatccatc agtcaacttc cacaggtaat
     4021 tgattaactt gtgatagatc tgatttctga gattatgtct taaatgtaat gattaaataa
     4081 tgttaaaaac aacttatttg attccttttt ggcagaagtt taatatctta cagtttagtt
     4141 tgtttaatta ttgattagaa gcttataata atatttttcg aagttaacat agaaccctta
     4201 aagctaacta attacaaatg agtatgaaaa aaataatttt tttcttgatt gtagctgtca
     4261 attgctggac agctctagcc caggtccctg gtttacaggg aggtcagcaa ccagcaccgc
     4321 ttccagaagg gtttaaacct tctgaatgta atacattcct gtcgcaatat cctgcagtta
     4381 atgcccaaac ccgtgaggca cttttccgtg ttgtcgcacc aggtgccaga attgtgcagg
     4441 ttgatcttgc aggcaaaaga tataatatgg taaaagatga gaaaggagtc tggacctgca
     4501 aatctgaccc acaggttgtg ggtttccatt attattccat ctggatcgac agtgttcctg
     4561 ttatggacag gaatacaaaa gcattcttcg gcagcaactg ggagtcgtca ggaattgaaa
     4621 ttcctgaagg gccagagggt gattattaca ggttcaataa aaatattcct cacggacata
     4681 ttcgttcaat gtattactgg tcggatatta atgggctgga acgccattgc aatgtatacg
     4741 ttcctgctga atatgaaaat aatcctcaga agaggtaccc ggtgctctat ctgcttcatg
     4801 gatggggaga ggatgaagac ggatgggtta atcaggggca tattgcaaac attatggatg
     4861 ggctgatagc atcgggtaag gcggttccaa tgattgtggt aatggattgt ggtgatttca
     4921 agacaaatcc agatgtgagg agggcttcca caaatgatat tacacagata tacgtcaaag
     4981 aattgatccc tttcatagat aagacgttcc gcaccaaaac tgaccgactt aaccgtgcaa
     5041 tggctggcct gagcagaggc ggcatgcaaa ccacaatgac tgttttcaat aaccctgaca
     5101 aatttgcctg gttaggaact ttcagcggtt tctttatggg tggatttggt aacaggacag
     5161 cagcaggcgg aggtcaggct ccaggcatga ctcagcaaat ccagacagct ttcaatggaa
     5221 ttttcaaaga ccctgaggct tttaataaac agtttaagct tctgttcatc agtaccggta
     5281 cggaagaacg cagccctaaa gaacaggttg aagctctaaa ggcatatggc attaaaaata
     5341 ttgtttttca tgaatcccag ggcacagcgc atgaatggct tacatggcgc agagcgttgt
     5401 atgactttgc tccaagaatt tttaaataga atgaatgaaa ttaaatgttg aaattctaac
     5461 gattatccag aatgaatgaa aagattaaag ccggcaacca ctgcatagct ctaattatca
     5521 cttttttagc tttattcgta acagtttcct gttacaggcg tgcagaattt accatcaacg
     5581 accggcggat atgctttgac gaagaatgga ggttcataag ggattcttta gccggagcag
     5641 aaaaccctga atacgatgat tcatcatggc ggttgcttga ccttccccac gactggagca
     5701 ttgaagacct gcccggacag aacggagttg atattatagg tccgtttgat aaaaatgctg
     5761 tcgacagaat ggcttcagga tataccgtgg gaggcaccgg ctggtacaga aagaaattca
     5821 agactacgga agaatacagg aataagcgtg tgtttcttga attcgacggg gtttatatga
     5881 acgccgacgt atggctgaat ggaaatcacc tcggatttca tccttatggt tatacacctt
     5941 ttcatttcga tattacacct tatctgaatg aaccgggaaa agagaatgtt gtagcagtac
     6001 gtgtgaggaa tgaaggactc aattcacgat ggtattcagg ttctggaatt tatcgtcatg
     6061 tatggataat ccccgttgat cccgttcata tagacatagc aggtggaata tttgtaacaa
     6121 caccggaggt taacccgaac tctgctcagg tagatgttgc tgtaacactt gttaatgacg
     6181 ggcaaaacga aaaaagcata acggtacaat ctgaactttt cgataagaaa gggaaaagtg
     6241 caggtaaaat tagcagcagt gatgtcacac tgactccagg cataacaaaa gtcatacata
     6301 agctaaatgt taaaaagcct ttgctctggt cgttggacga accaaatctc tacagggcaa
     6361 gagtcagtgt tatgtcagtt gatggtgaag tgattgatga atgtgaggca cctttcggca
     6421 tcaggattat taagattgat gctgtaaagg ggttaactgt aaatggaaaa actgtaaaaa
     6481 tgattggcgg gtgttttcat catgacaatg gtccgcttgg agcagttgcc atcgaccggg
     6541 ctgaggagag gaaaatagcg attcttaaaa agacgggatt caatgcaatc cgaacaagcc
     6601 ataaccctcc atcgacagag ttgctgaatg cctgcgacag gatcgggatg cttgttattg
     6661 atgaaatatt cgacatgtgg gagactccaa agagggagaa agattatcat cagtatttca
     6721 aggagtggtg gcagagagat gttgagtcgt gggttaagcg cgacaggaat catccttcgg
     6781 tagttatatg gagtatcggt aatgagatac gtgagacgta tgatactacg ggtttaagga
     6841 ttgcacgtaa tttaacgggt gagatacgac gtcttgaccc cacccgggct gttactgaag
     6901 ctttcaacga tttcggatgg cgcaggggtc agggcagcag gtggcatgaa attcccgagc
     6961 acatggcact gctggatgta ataggttata attatatgta tcagaggtat gaggaggatc
     7021 atgaaagata tcccgacagg gtgatggtgg ggacggagac caatccgcca cttgcactgg
     7081 agaactacga gattatgatg aagcatcctt atgttatagg atattttgta tggactgcaa
     7141 tagataatat aggagaggcc ggagtaggta tgccacagct cagggatgca gggcaggaag
     7201 ttcaggtgca gggtgctcct gccagtggac ctggggctgg ttttcagcag ggagtacagg
     7261 tgggcgctac aggcagggct ccgggaggtg taccggcagg tactcaacag gctgcacagg
     7321 gaacacccgg gatacccggg gcacctcagg gcatgccggg aggtgggttt ttcaggcggg
     7381 aaacatggcc ggtgtttaca aattaccagg gcgacctcga cctgacactc aacagaaaag
     7441 tcccttcata ctaccagtat gttgtttggg gtaagagtaa agtagagatg tttgtacacc
     7501 gccccatacc ggaaggaaaa gtagaggtga caagccgctg gggtttcccc gatgaggtta
     7561 aaagctggaa ctgggctggc agggaaggcc agaagtttca ggttcatgtt tatacccgtt
     7621 ctcaaaaagt aagactcgaa ctaaacggca aaatagtggg tgagcaggag gttgaccaga
     7681 ctaaatcgat aactgcaact tttgaggttc cttatgaacc cggtaccctt gttgcacgct
     7741 gcttcgacaa tggcagggag actgcttcgg agagacttaa aactactggc agaccggcag
     7801 caatcagact gacagccgac agaacggtta taagagcggg aaagaatgac ctttcatatg
     7861 tgacagcaga aatagttgac agtaatggtt ttgtggtgcc tgatgctgat agtattatgg
     7921 taaactttga ggttactggc aacggaaagg tggcaggtgt cggcagcggt gacccgaggg
     7981 acatgtcgag ctttcagcag ccacgcaaga agacctacga gggaagatgc atcgcagtta
     8041 ttaaacctgg cagggaaccg gggaaaatac gtgtacaggc tactgcagaa ggtctgagag
     8101 gagcatcggt tgtgataaca gttaagtgag gagaatttat ttgctttagg tcattcaccc
     8161 gggcaaacct ttttcggtcg gtcaggaata aatatcttca gcaggtattt aattgtgcag
     8221 tttttcagaa tatttaagcc cccctgccag gggggttggg ggggtgaacc acatcaagcc
     8281 ccctctttac gaagtagaga gggggttggg gggtgaacca catcaagccc ggttcggtcg
     8341 gtcaacaaaa aagcccttcc cggtaaaccg ggaaaggctt ttgacttgta gcgggggaag
     8401 gattcgaacc ttcgaccttt gggttatgag cccaacgagc tgccactgct ccaccccgca
     8461 gtattatttt tttatttaaa gaacctttct tcctcaggga ttcgaacctt cgacctttgg
     8521 gttatgagcc caacgtcccg atagctatcg ggaccacccc gcagtatcat tttttattta
     8581 aagaaccttc ttttctttaa ggtccgaacc ttcactcctt cctgataacc tggtagctta
     8641 tgttaactca cctcttagct atgccagttt tctaacccga aaccctacgg ttttgtgggt
     8701 acaaaattat aaaaattata attaatgtca aaacatatat ccttgatttc ataaagcaat
     8761 taccgagtta tattattttt gaagaaattt tgttttatta aatctttatc attaaaaagc
     8821 aaattaaccg ttatgccaag ggtattcaaa gcaacagaca ttgagaatga ggctaaggaa
     8881 tccagaagag attacttcga ccgccataca cctcatatta tttgtcctga taaagtaaaa
     8941 aaaggcgaaa agtttgaagt aaaagtcaaa ttaggtgaaa actatgtaca tccagatgat
     9001 cctgatcatt atataagcct gatacagttg tggaacaggg agacactcct tgccgaagta
     9061 cgttacactg ccggcgcata cggtaataag cccggacatt ttgaagtggg attctatatt
     9121 gtggctccag aagtcaatat gaacctatct gccatgtcgg tatgtacaaa acacggtctc
     9181 tggcaaagtg atgacaaacc tgtggaggtt atataactgg catccggttt gttaatataa
     9241 taatgtaaaa tacctcaggg gtgaggtttt tattatcttt gtatgctttt ttattcagtt
     9301 ttgtaaatgg caaaggaaga aaaagaaaag aaagaaaaaa agaagaatag ctggcgcgaa
     9361 aagtacaggt ttactattta taacgacaca acttttgaag aagtctggaa aatcaggctt
     9421 acccgttata atgcattcct ggtggtatct tttattgtcc tttttataat tggttcaacg
     9481 gtttctctta ttgcattcac caacctcagg gaatttatac ctggttatcc tgactctgag
     9541 atgcgccgca atatacttat gaatgctatc aggcttgatt cgcttgagcg ggagctcgat
     9601 ctgagagaca agtattttgc aaacctgaat gcaataattt cagggaaaca gccagttgat
     9661 atactcgcca cacccgatac tgggaaaaaa tacagcaaca ttcagttttc aagcatacct
     9721 gaagttgaaa cacttaaaac agaaatcgaa aaagaagagc gatttaacct tactttaccg
     9781 cggctgactg agcctgccgg acttgcaggg ttgcattttt tcccgcctgt gaaaggtata
     9841 attactgcaa aatttgatcc gcgtgcaaaa catttcggtg ttgatatagt taccaggcca
     9901 aagtcacctg tgatggctgc tctggatggc acggtagtat ttaccggatg gacaatggaa
     9961 accggctatg ttatacagat tcagcacagt aacaatttaa ttactgtgta caagcacaat
    10021 gcttctcttt taaaggaaaa cggcgattat gtgagagcgg gagaagcaat atcggtggta
    10081 ggaagttccg gagaaatgta tacgtcaggc cctcatctgc actttgaaat atggtacaag
    10141 ggaatacctc tcgatccgga aaaacacgtt ctcttttaag tagcaaataa attcactctg
    10201 caatgctgaa acccaaacgg atagcaattt tagggtcgac aggatcgatt ggcacacagg
    10261 tactggacat catttcaaga taccctggca gattcagacc ggaagtcctt acctgcaaga
    10321 gcagtgctga tttgctgata gaacaggcac ggagatacaa accagccaga gtggttattg
    10381 caaataaatc actttatgca agggtcaaag atggactgaa agaccttcct gttgaagtga
    10441 tggcaggtaa tgaagaactg gagtatgctg ccactctgga tgaaatcgac atggttgtgg
    10501 cagcgatggt tggatatgca ggaatgaaac cggttcttgc cgcagtaaaa gcgggtaaga
    10561 atatcgccct tgcaaacaag gagacactcg ttgtagcagg tgaactgatt ggtgagctca
    10621 ctgccagcta tcacgcttca attttgcctg tcgattctga acattcggca atttttcagt
    10681 gttt
//
LOCUS       DAOSNB010000049        10623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6232_length_10623_cov_3.928369, whole genome shotgun sequence.
ACCESSION   DAOSNB010000049 DAOSNB010000000
VERSION     DAOSNB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6232_length_10623_cov_3.928369"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1760
                     /locus_tag="PLG42_03520"
     CDS             66..1760
                     /locus_tag="PLG42_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU30313.1"
                     /translation="MKFSENQIRLFRNLSVVSGIFTMVIAFTMIMSLIQLKTSDPLED
                     PALKSIIEQFDKDPENADIAENVRSLDLIARKAYFAARWQLETGSYLLLAGAILFVVF
                     QWLSTGQEKSARGKLPPKPQITEEKERSKKYLIVFSSVFFILAVISSFALRPLLPAPG
                     EAKKGIRSEETGKASAVLPVPDGINYPNFRGEGSRGIAGGEGYPTDWDGNTGKNIKWK
                     VPVPKFGKSSPVIWGDKLFLTGAEGSVFEVYCYDKNTGDLLWTGTGSDFPGASKDVPE
                     TDEESGMAIQTPAVNKDAVCAIFANGNLVAYDHNGNLLWAKNLGMPEHTYGYTSSLLI
                     YNDILLVQYDSELRLSVIGFDVKSGKQLWETSRRGKPVNSSPALGMFNGIPQVIINGS
                     PEVTAFDVMTGKELWTINGVVNDVAVSPAVNNNFVYVSANYEALLAIKPDKSGATVGW
                     SDNTYTPDVSSPAATDEFLFISDGTGAIACYKADSKEKLWENYFNSSFYASPIVAGGM
                     VYFLDRDGVMHIVKASATYELVSESSIGEDTDSTPAFSEKKIYIRGEKNLYCISAD"
     gene            1842..2972
                     /locus_tag="PLG42_03525"
     CDS             1842..2972
                     /locus_tag="PLG42_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU30314.1"
                     /translation="MKKLKIYLISLITVWVSTTITAQNPDWPLFRGNPGLTGKTEWTL
                     PANPEVLWSFNSGDRTKSSPVISNGVIYFGTDKGSLVAVESNGKLKWKLGAGSPIEAP
                     PLISDNKVIYSTVDGTLRAADKETGKILWSYKTGNQIAGSANEWSSGKRTYLLVGSYD
                     FYLHCVDRETGKLMWKVETENYINGAPAISGSRVIFGGCDGMLRILDPLSGKQMKIVE
                     IGVYMAASPATIFPEAYIGDYDGNFYRVDIDKGEIKWYKTIGDGGSPVMSVPALSGNS
                     VVTGSGDNSIYCFNTADGSLRWSYRTNGSIEGSPIISGNNVLVGSTDGYVYLLSLSDG
                     SRKWSFNCGSSIISSPAVTQDRFYILTQDGRLIAFGKKTNRQ"
     gene            2969..4264
                     /locus_tag="PLG42_03530"
     CDS             2969..4264
                     /locus_tag="PLG42_03530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU30315.1"
                     /translation="MKIAFLVTGSGGSFYCSNCYRDMLYYNAIKKYSNSESVLIPLYL
                     PPEKEYARAGFEEEVYFGAVSLYMREKMPSLPSFFEKILDSKPLLKIAAKSSGSVRTA
                     GHEELTLHMIDGTYERHKKELERLVNHLREDIRPDIVHLSNALVIGLARQIKKYLDIK
                     IVCSLQNEDDWINEMAEPYQSQAWKMIAEESSFVDAFITPSLYFKDFFISKTGINSEK
                     IKVIRTGIESSDYLVPEVKKESPAIGYLSRVNYNNGFDKLVDAFIKIKSETELKPLKM
                     FVCGGFTGDDRPFLKEQLNKLHKYGFHDSVKICSDFHGEAKAEFLKSVDLISVPVRKP
                     DAFGLYVIEANAAGVPVVLPWSGAFPEIIEITGGGVLYKPDTVDELTENIIYMLRNKN
                     IMEEYGKTGMYNVGNKLTMEKMTEDMVNVYSSLVNKSTK"
     gene            4272..4940
                     /locus_tag="PLG42_03535"
     CDS             4272..4940
                     /locus_tag="PLG42_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU30316.1"
                     /translation="MLRVLELRKSYPQRGIVLNGLSLELKESETIAVTGPSGSGKTTL
                     LNLVGLLDRPDSGEILFREKLVNNYTVDEAADYRNRQIGFIFQDHLLLPYLTVMENIL
                     LPLSARQLEDDEYRVKVGYARELMERTGIMNLSSKYPWQVSGGEAQRAALVRALINKP
                     SLLLADEPTGSLDAANAEILGNLLKEISSQYGTAVILATHSMTLAKKMSIIYKIENGK
                     LVKI"
     gene            4957..8220
                     /locus_tag="PLG42_03540"
     CDS             4957..8220
                     /locus_tag="PLG42_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30317.1"
                     /translation="MKLSSRKLVLKSLIHYRKSAFNQAVIIMVLTAVFTGSLLTGYSV
                     RQSLRDSAGKRLGNTGLLISSGLRYFRPELDSKLTAVTGIKTASVLETEGSVEKFGNG
                     NRIENIKIYGIRHTFFTLNNVQGKFIDNVDAAINEHLARRLNLSIGDEIIVTHRQISD
                     LPSSSPFAPSAGNIASHVFKIKYIFTEEEAGNFSLEIKQTSPDNIFVNISELSRNGKA
                     NRILIEKDNSFSTDEILEKLKSILVPADLGLKVRHSERTGFNEILTGRIFFDSTLVKE
                     IKSIIPGASPVITYLVNSTEKEGRLNPYSFASAVERFIYPEVPEGNKILINEWLASDI
                     GAKKGDTLSLTWYDPDRISDFEENQGKFIVHDIVKIAGKWNDLELMPEFPGISGRQSC
                     SRWDAGMPLKTELIRKKDEEYWNIYKGTPKIFLNYETGKKIWASNFGSATAIRVPPDI
                     SRDEILNALTGSIEPVKCGFAIRNIRNDMINAANQSVDFSGLFIGLGFFIIASCFILL
                     IMAVNSFLDSRRSQIFSFFALGFSNRKIKKLLLAENLIISVAGCLAGVFAGIFFNEIV
                     VRALNGVWRGAVQTDTIKAVTGLVPSFAGFLAAFLTSITLQFIRVRKFLKEMQMGESR
                     EFKVINVKPELIRIISTLFITAGIILLLADYAILKEPLLSVSYSAGGLLFAGLILLWF
                     FLIIRKEAPLHKTVNSELAVSRSYYSFYPARAVMPVLLISAGLFAVIITGVNRLKVNE
                     SSLSQRGGTGGFLLWAETTVPVKENLNTKEGKKAHGLSYFPAETIFFLQAKRLQGDDA
                     SCLNLHHVTSPPLLGIDPSTLRERKAFSFSTLLKEANPSDPWEMLNRTPGENIIYGYA
                     DQTVLQWGLKMRVGDTLIYNAENGEKLYIIIAGGLKSSVFQGYVITGEEFFSRFFPSV
                     SGYSVFLAGGNPELTEDYRKILSARFNSYGINIMSTRERLEKFFEVTNTYLSVFMVLG
                     GLGMILGVIGLAFVLRQNYNFRRREFALMLATGFMVKKIRKILLKEQIIILVAGVITG
                     TLSALTATLPSVEKSTDIPLVSILTIIMAIIITGMTALLFAIRSIDKDYLISALRKE"
     gene            complement(8238..9587)
                     /locus_tag="PLG42_03545"
     CDS             complement(8238..9587)
                     /locus_tag="PLG42_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOU30318.1"
                     /translation="MKINILLLFTLLILNITTLSQENPARQWPWYRGYHISGVLDNAN
                     LPETFDFKTMNNIRWKTEIPGLGLSSPVIWGNRLFVTTALSKSDNKGFRTGIFGDVTS
                     VDDVSEHEWLVICFDKNNGNRIWERVAHRGVPAMKRHPKSTHANASVATDGKHVVAFF
                     GSEGLYCYDFNGNLLWKKDFGVLKSVFFSMPEAQWEFASSPIIYEGKIIIQCDVLENS
                     FVAVLDAASGKELWRVNRDEYPGWCTPNIYTFQGRKYVVVNGYKHRGAYELETGKEVW
                     RMSGGGDIPIPSPVIGKDIIYFNSAHGKSSPILAVKASARGDITLKEGETSNDYVLWS
                     RPRGGSYLQTLLLYRNHLYNLNWNGTINCYDPLTGEEIFSGKLGDAKSFSASPVASDG
                     KIYAVDEEGTIYILRDGNKYELIAEIPSGEVCMSAPAITDGMIFFRTQKYLYCVGKK"
     gene            complement(9671..>10623)
                     /locus_tag="PLG42_03550"
     CDS             complement(9671..>10623)
                     /locus_tag="PLG42_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOU30319.1"
                     /translation="LWLKKTKPETFSKVRYILHFPQYFSFMLSGQVVSEHTSLGCHTA
                     LWDFDNMKYHPWVHDENLRLPEPVSVFKLKEANISGKRVMVGTGLHDSSASLAPYFSP
                     DTGNFILVSTGTWCISMNPFNHEPLTTEELNQDCLCYMSINREPVKSSRLFLGHMHDE
                     AVGKISQYFKINENSFRLVKPDVKLLNRMMGKFMDEKIFTIPGSKPGALRDEPELFLF
                     DNFIEAYHQLMIELSDLTVEAINRAAGEDDKTENIFITGGFSKNPLFLKLVASSFPDK
                     RVYTSEISNATSLGTAMVTLRALMPDKEFKPDLGLTEITL"
BASE COUNT         3300 a         2011 c         2219 g         3093 t
ORIGIN      
        1 aagatgcgtt agttgtggaa aatgttttga atattgtccg gtgaaaatta aaactgaata
       61 gaaagatgaa attttcggaa aatcagatca gacttttcag aaatttaagt gttgtatcag
      121 gcatttttac aatggtaatt gcttttacca tgattatgag tctgatacag ttaaaaacat
      181 ctgatcctct tgaggatcca gccctgaaga gtattattga acagtttgac aaggatcctg
      241 aaaacgcaga tatagcagag aatgtaagat ctcttgacct tattgcgcgc aaagcatatt
      301 ttgcagcaag atggcaactg gaaacagggt catatctttt acttgcaggg gctattctgt
      361 tcgttgtttt ccagtggtta tcaacgggtc aggaaaaatc tgcaagggga aaattgcccc
      421 caaagcctca gataacggaa gaaaaggaac gaagcaagaa atatttgata gtattttctt
      481 cagtgttttt catcctggct gttatatcat catttgcttt acggcctctt ttgcctgctc
      541 cgggggaagc aaaaaaggga attaggtctg aagaaacagg taaagccagt gcggttttac
      601 ctgtacccga tggaataaat tacccgaatt tcagaggtga aggtagcaga ggaatagccg
      661 gtggagaagg gtatccgacc gactgggatg gaaataccgg gaagaacata aagtggaaag
      721 ttccggttcc aaaatttgga aaaagttcac ctgtcatctg gggtgataaa ttattcctaa
      781 ccggggctga aggcagcgta ttcgaagtat attgttatga taaaaacaca ggtgatttac
      841 tctggacagg cacaggctct gattttcccg gagcatcaaa agatgtacct gaaactgatg
      901 aggaatcagg aatggcgata caaacgcctg cagttaataa agatgctgtt tgcgccattt
      961 ttgccaacgg aaacctcgta gcctacgatc ataatggcaa tcttttatgg gccaaaaacc
     1021 tgggtatgcc tgaacataca tatggttaca catcttcgtt attaatttat aatgacatac
     1081 ttctggtaca atatgacagt gaattacgtt tgtctgtaat tggttttgat gtaaaatcag
     1141 gaaaacagct atgggaaaca agccgcagag gcaaaccggt taattcttca cctgcgctgg
     1201 gtatgtttaa tggaatacca caggtaatta ttaatggcag tcccgaagtg acggcatttg
     1261 atgtaatgac aggaaaagaa ttatggacaa tcaacggagt tgtaaatgat gtcgctgtat
     1321 caccagcggt taacaacaat tttgtttatg tatcggccaa ttacgaagca cttcttgcga
     1381 taaaaccaga taaatcaggg gcaacagtcg ggtggtccga caacacatat acccccgatg
     1441 tatcaagtcc ggctgcaact gatgaatttc tatttatctc tgatggaaca ggtgctattg
     1501 cctgttataa agccgacagc aaagagaaat tatgggaaaa ctattttaac agttctttct
     1561 acgcttcacc gatagtagca ggaggaatgg tttatttcct cgacagggat ggggtaatgc
     1621 atattgtgaa agcatcagca acatacgagc tggtgtcaga atcctcaatt ggagaagaca
     1681 ctgacagcac ccctgctttt tcggaaaaga aaatatatat aagaggggag aaaaatcttt
     1741 attgtatttc tgcagactga actatttatt ttattttatt ttattatgac aggttaaagt
     1801 taaagttaca tcctgagaag aaattcaggg cagtgaggaa aatgaaaaag ttaaaaatat
     1861 atctgatttc acttattaca gtttgggtat caacaacgat taccgcgcaa aaccccgact
     1921 ggccattatt tagaggcaat cccggcctta ccgggaaaac ggaatggact ttacccgcca
     1981 atcctgaagt attgtggagt ttcaattcag gagatagaac aaaatcttct ccggtaatca
     2041 gtaatggcgt tatttatttc ggaaccgata aaggttctct tgtagcagtt gaaagcaatg
     2101 gcaagcttaa atggaaactt ggagccggta gccctatcga agctccgcct cttatttcag
     2161 ataacaaagt tatctacagt actgttgacg gaacattgag agctgctgac aaagaaaccg
     2221 gaaaaatttt atggtcgtac aaaaccggta accagatagc aggatctgca aatgaatgga
     2281 gctctggtaa aagaacatat ttacttgtag ggagttacga tttttatctt cattgcgtcg
     2341 acagagaaac tggtaaactg atgtggaagg ttgaaactga aaattatata aacggagctc
     2401 ctgctatctc tggtagcaga gtaattttcg gaggatgtga cggaatgctg aggatacttg
     2461 accctcttag cggcaaacaa atgaaaattg ttgaaatagg tgtatatatg gctgcttccc
     2521 ctgctactat attccccgaa gcttatatag gcgattacga tggtaatttt taccgggtgg
     2581 atatagataa aggggaaata aaatggtata aaactattgg tgatggcgga agccctgtta
     2641 tgtcagtccc tgcattatct ggcaacagcg tggttacagg aagcggagat aactcaatat
     2701 attgttttaa cactgccgac gggagcctga ggtggagtta ccgtacaaac ggaagcatag
     2761 aaggttcccc gataatatct ggaaacaatg ttcttgtggg aagtaccgat ggttatgttt
     2821 acctgttgag tctttcagat ggcagcagaa aatggagttt taactgtgga agttctataa
     2881 tttcttctcc tgcagttaca caggaccggt tttatattct tacacaagac ggaaggctta
     2941 ttgcttttgg aaaaaaaacc aatagacaat gaaaatcgct tttctggtta caggctctgg
     3001 cggctcgttt tattgcagta attgctaccg cgacatgctg tattacaatg caataaaaaa
     3061 gtacagtaat tcagaatctg tgttaattcc cctctacctc cctcccgaaa aggaatatgc
     3121 cagagctgga tttgaagaag aggtctattt tggagccgtt tcattatata tgagagagaa
     3181 gatgccatcg ttgccatcat tctttgaaaa aatacttgac tccaagccac ttctgaagat
     3241 tgcagcaaaa agctcaggct cagtaagaac agcaggtcat gaagaactga cactgcatat
     3301 gattgacggc acctacgaaa ggcataaaaa ggaacttgaa cgattggtaa atcacctgcg
     3361 ggaagatata agacctgata tcgtacatct ttcaaatgcg ctggtaatcg gattagccag
     3421 acagataaaa aaatatcttg atatcaaaat agtatgctcc ctgcagaatg aagatgactg
     3481 gataaatgaa atggcagaac cttaccagtc gcaggcgtgg aaaatgatag cagaagaatc
     3541 atctttcgtt gatgctttca ttactccgag cctctatttc aaggattttt tcatttccaa
     3601 aacaggtatt aactctgaaa aaataaaagt tataagaact ggtattgaaa gcagtgatta
     3661 tcttgttccg gaagttaaaa aagaatctcc agcaataggc tatctgtcaa gagtgaatta
     3721 taacaatggg tttgataaac tagtagatgc ctttattaaa attaaatctg aaactgagtt
     3781 aaaaccatta aagatgtttg tatgtggtgg ctttactggc gacgacaggc ctttccttaa
     3841 agaacagctc aataagctgc ataaatacgg atttcatgat tcagttaaaa tttgcagtga
     3901 tttccatggg gaagcaaaag cagaattcct caaatcagta gatctgataa gtgtccctgt
     3961 tcgcaaacct gatgcattcg gtctttatgt gatagaagca aatgcagcag gtgtaccggt
     4021 agttcttcca tggtcgggag cttttccgga gataattgaa attacaggtg gcggagtact
     4081 ctacaaaccc gatacagttg atgaacttac cgaaaatatc atttatatgc tcaggaataa
     4141 aaatatcatg gaggaatacg gcaaaactgg catgtataat gtgggtaaca aactgactat
     4201 ggaaaagatg acagaagaca tggttaatgt gtacagttcc cttgttaaca agtcaaccaa
     4261 ataatatagt gatgttaaga gtactggaac tcaggaaatc ttatcctcaa agaggtattg
     4321 tactcaatgg tctcagcctt gaattgaaag aaagtgaaac aatagccgtc acaggtccat
     4381 ccggttctgg caagacaacg ttacttaatc ttgtcggtct gctagataga cctgattcag
     4441 gagaaatttt attcagagaa aagctcgtta acaattatac agttgatgag gctgcagatt
     4501 accgtaacag gcagattgga ttcatttttc aggaccacct gcttcttccc tatctcaccg
     4561 taatggaaaa catacttctt cctctgtcag caagacaatt agaggatgat gaatatcgtg
     4621 taaaagtcgg atatgccagg gaacttatgg agcgtaccgg aataatgaac ctatcctcca
     4681 aatatccctg gcaggtttca ggaggtgaag cacagcgtgc ggctcttgtc agggctctga
     4741 ttaataaacc ttctcttctg ctggcagatg aacccaccgg ttcccttgat gctgcaaatg
     4801 cagaaattct gggcaatctg ctgaaggaaa ttagttcgca atacggaact gctgtaatac
     4861 tggcgactca ttcaatgaca ctggcaaaga aaatgtcgat tatttacaaa atagagaatg
     4921 ggaaacttgt aaaaatctga atttgtttaa cataagatga aactaagcag cagaaaactt
     4981 gttctgaaat ctttaattca ttacagaaaa tctgcattta accaggcagt gataataatg
     5041 gttctgactg ctgtttttac aggttcactg cttacaggtt attccgttcg tcaaagcctg
     5101 agagattcag ctggaaaacg ccttggaaat accggtttgc ttatcagttc gggtttaaga
     5161 tatttcagac cggaactcga cagtaagctc actgccgtca ccggaattaa aacagcttca
     5221 gtgctcgaaa ctgaaggatc tgtagaaaaa ttcggaaatg gaaacagaat tgaaaacatt
     5281 aaaatatatg gcatcaggca tacttttttc acattaaata atgtgcaggg taagttcatt
     5341 gacaatgttg atgcagcaat aaatgaacat ctcgcaagga gattgaatct ttctattggg
     5401 gatgaaataa ttgttaccca ccgtcagatt tcagacctgc cttcttcatc gcccttcgct
     5461 ccatcggcag gtaatattgc ctcacatgtt tttaaaatca aatatatttt tactgaagaa
     5521 gaagcaggga atttttcact cgagataaaa cagacctctc ctgataacat ttttgtcaat
     5581 atttcagaac taagcaggaa cgggaaagct aaccggattc ttatagaaaa agacaattca
     5641 ttttcaactg acgagatcct tgaaaagctg aaaagtattc ttgtgccggc cgatctgggt
     5701 ttgaaagtga gacatagtga aagaacagga tttaatgaaa tattgacagg cagaatcttt
     5761 ttcgacagca cacttgttaa agaaataaaa agtataattc ctggtgcgtc acctgtgata
     5821 acctatctgg ttaacagcac cgaaaaagaa ggcagactca atccttattc ctttgcctct
     5881 gcagtcgaac gttttatata tcccgaagtc cctgaaggca ataaaattct tattaatgaa
     5941 tggctcgcca gtgatattgg tgcgaaaaaa ggtgataccc tgtcattaac atggtatgat
     6001 cctgacagga tttcagattt tgaagaaaac cagggcaagt ttattgtgca tgatattgtg
     6061 aaaatagcgg gaaaatggaa tgacctggag ttaatgcctg aatttcccgg tatctcagga
     6121 cggcaatcat gcagcagatg ggatgctggt atgccgttaa aaacagagct tatcagaaag
     6181 aaagatgagg aatactggaa tatttataaa ggcactccca aaatattttt aaattatgaa
     6241 accgggaaaa agatatgggc aagtaatttt ggttctgcaa ctgccatcag ggtgccaccg
     6301 gatataagca gggatgaaat cttaaatgca ctcacaggtt caattgaacc tgttaaatgt
     6361 ggtttcgcta tacggaatat ccggaatgat atgataaatg ctgccaacca gagcgttgat
     6421 ttcagcggat tgtttatcgg acttggattt tttataattg cttcctgctt tattcttctg
     6481 ataatggcag tcaactcttt ccttgactca cgccgaagcc agatattttc attctttgca
     6541 ctgggtttca gcaacaggaa aatcaaaaag ctgcttttag ctgaaaacct gattatttct
     6601 gttgcaggat gtctggcagg agtctttgcc ggaatatttt ttaacgaaat tgtagtaaga
     6661 gctcttaacg gagtatggag aggagctgtg caaaccgaca caattaaagc agtaacagga
     6721 ttagttcctt catttgcagg ctttttagca gctttcctca cgtcaatcac acttcagttt
     6781 atcagggtta gaaaatttct taaggagatg cagatgggag aaagcaggga attcaaagta
     6841 attaacgtca aaccagaatt aatcaggata atctccactc tttttattac agcaggaatt
     6901 atccttctgc tggctgatta tgcaattctt aaggaacctt tattgtcagt atcgtacagc
     6961 gcaggcggat tattatttgc aggtctgatt ttattgtggt tttttctcat catcaggaaa
     7021 gaagctcctt tgcataaaac ggtgaattca gaacttgctg tttccaggag ttattactcc
     7081 ttttaccctg caagggctgt aatgcctgtc ctcctgatat cagcaggtct ttttgctgtt
     7141 ataataactg gagtcaacag gttgaaggta aatgaaagca gcctttcgca gcgtggagga
     7201 accggtggtt ttcttttatg ggcagaaaca actgtaccgg tgaaagaaaa ccttaataca
     7261 aaagaaggca aaaaggcaca tgggctcagc tattttcctg cagaaacaat ttttttcctt
     7321 caagcaaaaa gactgcaggg tgatgacgcc agctgtctta atcttcatca cgttaccagt
     7381 cccccgcttc ttggaataga cccatcaaca cttcgcgaac gaaaagcatt tagtttttca
     7441 accctgctga aggaagctaa cccttccgac ccatgggaaa tgcttaatag aacccccgga
     7501 gaaaatataa tatatggtta tgccgaccag acagtactcc agtggggctt aaaaatgcgt
     7561 gtaggtgata cacttattta taatgctgaa aatggtgaaa aactttatat aattattgcc
     7621 ggaggtttaa aatcgtctgt atttcaaggt tatgtaatca caggtgaaga atttttcagc
     7681 agattctttc cttctgtgtc aggatattca gtttttcttg caggcggaaa cccggaactt
     7741 actgaagatt acagaaaaat tttaagtgcc aggtttaaca gttatggaat caatatcatg
     7801 agcaccagag aacgtctcga aaaatttttc gaggtaacca atacatatct ttcagtattt
     7861 atggtacttg gaggattggg aatgattctc ggtgttatcg ggcttgcatt tgtactcagg
     7921 cagaactaca acttccggag gagggaattt gcacttatgc tggctactgg ttttatggtt
     7981 aaaaagatta gaaaaattct tcttaaagaa caaattataa tactggtagc tggtgtaata
     8041 acaggaactc tttcagcact tacagctaca ttgccttcgg tagaaaaaag taccgacatc
     8101 cctctggtgt cgattcttac tattataatg gcaataatta ttaccgggat gactgccctg
     8161 ctttttgcaa tccgtagtat tgacaaagat tatctgatat ctgctttaag aaaagaataa
     8221 attaattctt tctgtaatca ttttttgccg acacaataca ggtatttttg tgtcctgaaa
     8281 aaaatcatcc cgtcggttat tgctggtgct gacatacaaa cttcacctga aggtatttct
     8341 gcaattagtt cgtatttgtt gccatcacgg agaatataaa ttgtaccttc ttcatcaact
     8401 gcatatattt ttccgtctga tgccactggc gaagctgaaa agctttttgc atccccgagt
     8461 ttacctgaga agatttcttc tccggttaaa ggatcataac aattaatggt accattccag
     8521 ttaagattat aaaggtggtt tctgtataaa agaagtgtct gcaggtaaga tccaccacga
     8581 ggtcggctcc ataaaacgta atcatttgat gtttctcctt ccttaagtgt gatatcacct
     8641 ctggcacttg cttttactgc cagaataggt gaacttttac catgcgcact gttaaaataa
     8701 ataatatctt ttccaatcac cggtgatggt atcggaatat cacctccacc tgacatgcgc
     8761 catacttctt tgcctgtttc aagttcgtag gcaccacggt gtttataacc gtttacaaca
     8821 acatatttcc tgccctggaa agtatatatg ttaggtgtgc accatcctgg gtattcatca
     8881 cggtttaccc tccataattc tttacctgaa gcagcatcca gtacagccac gaatgagttt
     8941 tccagaacat cacattggat aattatcttg ccctcatata taattggtga acttgcaaat
     9001 tcccattgtg cttcaggcat gctaaagaaa actgatttca gcactccgaa atcttttttc
     9061 cacaaaagat tcccattaaa atcgtaacaa tatagacctt ctgagccaaa aaaagcaaca
     9121 acatgttttc cgtcagtagc aactgaagcg ttggcatgag ttgatttagg atgtcttttc
     9181 attgccggta cacctctgtg agccactctt tcccagatac ggttaccatt gttcttatcg
     9241 aaacatataa caagccactc atgttctgat acatcatcaa cagatgttac atctccaaaa
     9301 attccagtcc ggaatccttt attatcagac ttgcttaatg cggtagtaac aaaaagcctg
     9361 tttccccata ttacaggact agaaagtcca agccccggta tttctgtttt ccatctgata
     9421 ttattcatgg ttttaaaatc aaacgtttcg ggcaggtttg cattatcaag aacacccgaa
     9481 atatggtaac ctctgtacca tggccactga cgtgcaggat tttcctgaga taatgttgta
     9541 atattcagaa tcagtaatgt aaacaggagc aggatattta ttttcatttt agcaataatt
     9601 taatattagc acatgtatcg aaacaaacac aattctccag gattgtgtta ttggtattaa
     9661 atagttcgtt tcacaaagta atctctgtga gtcccagatc cggtttaaac tccttatctg
     9721 gcatcagggc tctgagtgta accattgctg ttcccagaga tgtggcattg cttatttcag
     9781 acgtgtatac ccttttatcg ggaaatgaac ttgcaacaag tttcagaaag agcgggtttt
     9841 tcgaaaatcc acctgtaatg aaaatgtttt cagttttatc atcctcccct gctgccctgt
     9901 ttatagcttc tacagtaagg tcagaaagct caatcatcaa ctggtgataa gcctcaataa
     9961 aattgtcgaa caagaaaagt tccggttcat cccgcagtgc gccaggtttg cttccaggaa
    10021 ttgtaaaaat cttctcatcc atgaatttcc ccatcatcct gttaagcagt ttgacatcag
    10081 gttttaccag acgaaaagaa ttctcgttta tcttaaaata ctggctgatt ttccccactg
    10141 cttcatcatg catatgtccg aggaaaagtc tggaagattt taccggttcc ctgttgatac
    10201 tcatgtagca gagacaatcc tggttaagct cttccgttgt aagtggttca tgattgaacg
    10261 gattcatgct gatacaccag gtgcctgttg aaaccagtat aaagttgcct gtgtcagggc
    10321 tgaaatatgg tgcaagtgat gccgaactat catgtaaacc cgtcccaacc attaccctct
    10381 tacccgaaat atttgcttcc ttaagcttaa aaaccgaaac aggttcggga agtcgcaggt
    10441 tctcatcatg cacccacgga tgatatttca tattgtcaaa atcccacagt gcagtatggc
    10501 aacccagtga tgtatgttca gaaactacct gccccgacag cataaatgag aagtactgcg
    10561 gaaaatgcag gatgtacctt acttttgaga atgtttcagg ttttgtcttt ttcagccata
    10621 gaa
//
LOCUS       DAOSNB010000050        10385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6430_length_10385_cov_4.623427, whole genome shotgun sequence.
ACCESSION   DAOSNB010000050 DAOSNB010000000
VERSION     DAOSNB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6430_length_10385_cov_4.623427"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLG42_03555"
     CDS             complement(<1..807)
                     /locus_tag="PLG42_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOU30320.1"
                     /translation="MKKFLKWFFPGLLVGLLIYWGFEKALVLTSTNSFCMSCHIHPEA
                     DNSWKRSTHYETRSGYRIACVECHLPPKGQGYLMEKARTGLRDLWSYWTKDSASFDWV
                     ERRRLENARMYTYESSCIKCHENLFPLTLSKEGADAHLYYKQTKKTPDLHCINCHLNA
                     GHYIEGYTHGSNKTFGQVSSAPKEIFTEPAKVTKFESFTEQIPGSSVSFRMVAIPGGR
                     FLMGSPPDEPFRRSDEGPVREVQVSPFFMGEIEVTWDEYLAFYAQTAAEGR"
     gene            949..2373
                     /locus_tag="PLG42_03560"
     CDS             949..2373
                     /locus_tag="PLG42_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOU30321.1"
                     /translation="MKKIFALFLALILNFQLHSHPWKPSNYVIIDTDAGIDDIRAICL
                     LLASPDVRVLAITVSSGVLSPDNAYVKIKSLLNSFYHEGIPVGINRNNASSAQNLATA
                     LNYEWGNEKDIDPGKAPDYLKVISDILSYEKTKITFICLGGLNSAYNAIRTIPGFSSQ
                     IKNIVWSSNGLNDTKGFNYALDSKSAGSVLKGSVPVITVNTSGNRFFYNDNLLSEIAS
                     IKNPYSEKVNTFMQSSTAKNHSFSYEINDELIPVYLHYPENFRTTPSGNNLECIAENI
                     SGISEGYLRIIKGETVNRYQVIKKFPVDPSFYYDDINPYVTEIIGKYGMEEWQSGVLA
                     CEMHRHLGVYAIIGVKMGIRVREYFNTGVDEFEVVTWAGSVSPLSCMNDGIQLSTGAT
                     PGHGLLTVKNDSPAPVAEFSYLNKKIRITLKPEINQKVAGELKELNFVYGLDSNIYWE
                     LVREKAIKYWRDFDRHDIFIIEEL"
     assembly_gap    2567..2666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2851..4461)
                     /locus_tag="PLG42_03565"
     CDS             complement(2851..4461)
                     /locus_tag="PLG42_03565"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOU30322.1"
                     /translation="MAGFIYEKMFQTGEDRTLYRLLTEDFVSTIEFDGRKILKVDPEG
                     LVLLAREAINDLSFFLRSSHLEKLRRIIDDPEASSNDRFVAYTMLKNSVISAKGVLPW
                     CQDTGTAIVIAKKGENVYTGVDDAYYLSKGIFETYRDKNLRYSQIIPLSMFDEKNSGT
                     NLPAQIDIYSTEGDTYEFLFITKGGGSANKSLLYQQSKALLNEESLTKFIKEKIKELG
                     TSACPPYHLAVVIGGTSAEMTLKTVKEASAGFLDDLPTTGNESGRAFRDIEWEKKIEK
                     ICQEYGVGAQFGGKYFVHDVRVIRLPRHAASCPVGIGVSCSAHRNLKARITSEGIFIE
                     KLEKDPARFMPESVPETDKPVEINLDRPMKEILAELSKYPVKTRLSLTGTLIVARDAA
                     HARIKKMLDEGKPMPEYFKNHPVYYAGPAKTPAGMPSGSFGPTTAGRMDDYVELFQSH
                     GGSMIMLAKGNRSRKVIESCRKYGGFYLGSIGGPAAILAEENIKSTELVDFEDLGMEA
                     VRKIWVKNMPAFILTDDKGNDFFDEYNK"
     gene            complement(4565..5407)
                     /locus_tag="PLG42_03570"
     CDS             complement(4565..5407)
                     /locus_tag="PLG42_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU30323.1"
                     /translation="MKLHLFNISNFKVDGGAMFGVIPKALWSKAYPADENNLVTLALR
                     SLVIETGGRVILVDTGWGNKQDERFFRYLHIHDGEGLLEGLAARGFAPEDITDVFLTH
                     LHADHCGGAVRNGPRQGSYELTFPEATYHVSRAQWEWAVKGNPREEDAYPEENFLPLA
                     QNGHLNLVDNEGELFPGVSVIFCYGHTPGLMIPIIKYKDRTIVYTGDLIPTAAHIPLL
                     WNMSYDIESLKTIAEKERLCREAIKFGYILLFQHDIFVECCNLKDTPKGARMNEKFHL
                     EELL"
     gene            5628..6491
                     /locus_tag="PLG42_03575"
     CDS             5628..6491
                     /locus_tag="PLG42_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOU30324.1"
                     /translation="MKNTAILLAVIFTVQCINAQTGKVMDNLVMKSKILNMDRKYAVY
                     LPPDYETSQRSYPVLYLLHGAGDDQTGWVQFGEVLHIADEAIRNGKATPMVIIMPDAN
                     TGRRGYVNDIKNEWRYEDFFFEEFMPYVEKTYRIKSEKRYRAIAGLSMGGEGTFIYAL
                     HHPELFSSACPLSAATGPRNVEEMQNYRLWGLADNVTQEQKEAYFKKYSTLYLIENMP
                     DNQKRAVRWYIDCGDDDFLYEGNSLVHIAMRKKEIPHEFRIRDGGHTWTYWRSALPTV
                     LEFVSMSFHQF"
     gene            6495..8312
                     /locus_tag="PLG42_03580"
     CDS             6495..8312
                     /locus_tag="PLG42_03580"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU30325.1"
                     /translation="MKQVLFALFLIIAVSLHTEAQFVTEFIPPVRVVKVAEDAWFADF
                     GKDAFGTLVLNLKVKLPDTLIIRLGEKLTEPCKIDRKPGGSVRYAEVKLPVDPSREKY
                     IIKLPADKRNTSPPAVALPDTFGVIMPFRYCEIENYSGVITGDNIRQKAYFWKFNPEA
                     SAFSSSDTILNRVWDLCKYSIKATSFAGLYIDGDRERIPYEADAYINQLSHYAVDSEY
                     TLARRTMEHFINNPTWPTEWILHTVMIFYQDYMYTGDISLFSKYYEQLKAKTLVDLAR
                     DDGLISSKSPKLNGEMMRRLGFRDTTQRIRDIVDWPPAQKDTGWKLATPEGERDGYDM
                     KDINTVVNCFHYINLEIMSEIAGRLGKKEDSLWFANRAALVKTSINNKLFDSRKGIYI
                     DGEGSEHSSIHANMLPLAFGIVPENAINSVLNFIKSRGMACSVYGAQYLLEGLYNAGE
                     ADYALSLLTSTTDRSWWNMIRSGSTITMEAWDMKYKPNSDWNHAWGSAPANIIVRYLW
                     GIKPLEPGFARAEIKPQPGSLKWSTITAPTIKGQIKASFRKKRGSEKYLIDIPEGMKA
                     EFVPPPSLYKWIVLNGKKYDATINRISLKTGKNIIILKQ"
     gene            8319..9674
                     /locus_tag="PLG42_03585"
     CDS             8319..9674
                     /locus_tag="PLG42_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOU30326.1"
                     /translation="MRIHFIAIGGAVMHNLAIALHRNGHMVTGSDDEIYEPSRSNLAA
                     YGLLPPSAGWFPDKIHSGLDAVILGMHARSDNPELKRAQELGIKIMSFPEFLYDQTRN
                     KKRIVVAGSHGKTTVTSMIMHVFRHAGMKFSYMAGSSLKGFDLMVGLSDDSEIAVFEG
                     DEYPASALDKRPKFLLYKPDIAILNGISWDHMNVYKTEEEYIHQFRIFAESVSEGGTL
                     IYFKDDPLVNRIASEVKGDINKIPYRVHGYFINKIGVFGATHNRVIPLKFFGEHNMQN
                     LSAAREACFQAGITEDQFYEAIQSFEGAAGRLQILKKSDRGIVFYDFAHSPSKVKATI
                     EAVAETFAGKRIVACLELHTYSSLNAGFLPQYRNTMDRASVPYVFFSPHAVELKKLPP
                     LNPDIITGAFCNNSLRVFSNADDLFSDILREKQNDTIYLFMSSGNFDGYNLKKLADEL
                     V"
     gene            9752..10210
                     /locus_tag="PLG42_03590"
     CDS             9752..10210
                     /locus_tag="PLG42_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30327.1"
                     /translation="MKKIFVFVVFIALISFSTFAQEYKTSLGLRAGWPYGLTVKHFIS
                     RTSALEGILASSWHGFVVTGLYENENWTGSYPGLNWFWGVGAHVGFWDDNPHVNTTGS
                     VIGLDGILGLEYTFDEIPLNLSLDILPSFNIFGATGWGGIGGALSIRYVF"
BASE COUNT         3117 a         2220 c         2038 g         2910 t
ORIGIN      
        1 cctgccctct gctgccgtct gagcataaaa agcaagatac tcatcccatg taacttcaat
       61 ctcacccata aaaaacggac taacctgcac ctcccgtacc ggtccttcat cgctgcggcg
      121 gaatggctca tcaggcggac ttcccatcag aaacctgcct ccaggtatgg caaccatcct
      181 gaaagaaacc gaactacccg gtatctgctc ggtaaacgac tcaaacttcg taacctttgc
      241 aggctcggtg aaaatctctt tcggcgctga agatacctga ccaaaagttt tgttgcttcc
      301 gtgtgtataa ccctctatat aatgaccggc atttaaatga cagttaatgc aatgaagatc
      361 cggtgtcttc ttggtttgct tataatataa atgagcatct gctccttcct tgctaagagt
      421 aagcgggaaa agattctcat ggcattttat gcaactgctc tcatatgtgt acattctggc
      481 attctcaaga cggcgccgct ctacccagtc aaatgatgca gaatctttcg tccagtaact
      541 ccataaatcc cgcaaccctg tccgtgcctt ttccataaga tacccctgcc ccttcggcgg
      601 aagatgacac tctacacacg caatccggta tcccgaccgg gtctcataat gtgtcgacct
      661 tttccatgaa ttatcagcct cggggtgaat atgacacgac atgcagaaac tgttagttga
      721 cgtcaagaca agagcttttt caaatcccca gtaaatcagc aaaccgacta aaagcccggg
      781 gaaaaaccat ttcaaaaact ttttcataaa tctgataaat ctgaaaaatc aagggcatga
      841 atataagaaa attttttctg catcacgctt ttttctacct tctgcattaa tttcattaaa
      901 atattttgaa aaagtattac ttttaccgaa aatcgtaata ccattaaaat gaaaaaaatc
      961 tttgcgcttt ttctcgcatt aatccttaat ttccagcttc attcacatcc atggaaacca
     1021 tcaaattatg ttattattga taccgacgct ggtattgacg atatcagagc aatatgcttg
     1081 ctgctggcat cccctgatgt gagagtgctt gcaattaccg tttcttcagg tgttcttagc
     1141 ccggataatg catacgtaaa gattaaaagt ctgttgaact cattctacca tgaaggtatt
     1201 cctgtaggta taaatcgaaa taatgccagt tctgcacaga accttgctac tgcccttaat
     1261 tatgaatggg gaaatgaaaa agacatagac ccgggcaaag ctcctgatta ccttaaggtg
     1321 atatctgata ttctaagtta tgaaaaaaca aaaataacat tcatctgcct cggaggtctg
     1381 aatagcgcct acaatgcaat cagaacaatt cccggctttt cttcccagat aaaaaatata
     1441 gtctggagca gcaatgggct taatgacaca aaaggattta attatgcact cgatagcaaa
     1501 tcggcagggt cggtcctgaa aggtagtgtt ccagttataa ctgttaatac atccggcaac
     1561 cgttttttct ataatgataa tcttctgtca gaaattgcat caattaaaaa cccttatagc
     1621 gaaaaggtta acacttttat gcagtcatct acagcaaaaa accatagctt ttcttatgag
     1681 ataaacgatg aactgatacc tgtgtatctt cactatcccg aaaatttcag aactacacca
     1741 tcaggtaata acttagagtg catagctgaa aatatatcgg gcatatcgga aggatatctg
     1801 agaattatta aaggagaaac tgtcaaccgg tatcaggtta ttaagaagtt tcccgttgat
     1861 ccgtctttct attacgacga tataaaccct tatgttactg agataatagg caaatatgga
     1921 atggaagagt ggcaatcggg tgtacttgcc tgtgagatgc acagacatct cggagtatat
     1981 gcaattatag gcgtaaagat gggtatcagg gtaagagaat atttcaacac cggtgttgat
     2041 gagtttgaag ttgtaacgtg ggcaggttcc gtttcaccac tgagttgtat gaatgatggc
     2101 attcagctga gtaccggtgc aacccccggt cacggcctac tgaccgttaa gaatgattca
     2161 cctgctccag tagctgagtt cagttacctg aataaaaaaa tcagaatcac ccttaagccc
     2221 gaaatcaatc agaaagtggc tggcgaactt aaggaactta attttgttta cggcctcgac
     2281 agcaatattt actgggaact ggtaagagaa aaagccataa aatactggcg cgacttcgac
     2341 cgccacgata tattcattat cgaagagtta taaaaatgaa cctttgaaca attgaacctt
     2401 cgaacaattg aaccttagaa caattgaaca attgaacaat tgaacaattg aacctttgaa
     2461 cctttgaaca attgaacaat tgaacaattg aacctttgaa caattgaacc tttgaacaat
     2521 tgaacaattg aacaattgaa caattgaaca attgaacaat tgaacannnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnattg aacaattgaa caattgaaca attgaacaat
     2701 tgaacaattg aacctttgaa ccattgaaca attgaaccat tgaacaattg aaccattgaa
     2761 ccattgaacc attgaacaat tgaacctttg aaccattgaa cctttgaacc attgaaccat
     2821 cgaaccattg aacctttgaa cctttacacc ttatttatta tattcatcaa aaaagtcatt
     2881 tcctttatca tcggttagga taaaggctgg catattcttg acccatattt ttcttactgc
     2941 ctccatccca aggtcctcaa aatctacaag ttctgtcgat tttatatttt cttccgcaag
     3001 aattgctgca gggcctccta ttgagcccag ataaaatcca ccgtattttc tgcaggattc
     3061 aattaccttt ctgcttcggt ttccttttgc gagcatgatc atcgagcctc catggctctg
     3121 gaaaagctcc acatagtcat ccatgcgtcc ggcagtggta ggaccaaacg agccggaagg
     3181 catccctgcg ggtgtctttg ccggtcctgc ataatataca ggatgattct tgaagtattc
     3241 gggcattggt ttgccttcat caagcatttt cttgattctc gcatgggcag catctcttgc
     3301 tacgataagt gttccggtaa gacttaacct ggtctttacc ggatattttg agagttcagc
     3361 aagaatttcc ttcattgggc ggtcgagatt gatctcaaca ggtttatctg tctccggaac
     3421 actttcaggc ataaaccttg ccgggtcttt ttcaagtttc tcaataaaaa taccttcgga
     3481 agtaatcctg gctttcagat ttctatgggc actgcagctg actcctatgc ctacagggca
     3541 tgatgcggca tgcctcggta gtcttatcac cctgacatca tgaacaaaat actttccgcc
     3601 aaactgtgca ccaaccccgt actcctggca tatcttttca attttcttct cccattcaat
     3661 gtctctgaaa gccctgccgc tttcattccc cgttgtggga aggtcatcaa ggaatcctgc
     3721 tgaggcttcc ttcactgtct tcagggtcat ctcagcagat gtaccaccta tcacaacagc
     3781 gaggtgatat ggtgggcaag ctgaagtacc tagctcttta attttttcct ttataaactt
     3841 tgtaagtgat tcctcattta gcaaagcctt cgactgctga taaagaagcg acttgttcgc
     3901 agagcccccg ccctttgtga taaacaaaaa ctcataagtg tctccttctg tggagtaaat
     3961 atctatctgt gccggaaggt ttgttccgga attcttttca tcaaacattg aaagcggaat
     4021 aatctgtgaa tacctgagat ttttatccct gtaggtttca aaaatacctt ttgagagata
     4081 ataggcgtca tccaccccgg tatatacatt ttcgcccttc ttagcaatta caatagcagt
     4141 tccggtatcc tggcaccatg gtaacactcc ttttgcggag atgaccgaat tcttcagcat
     4201 ggtataggct acaaacctgt cattcgacga agcttcggga tcatctatta tcctcctcaa
     4261 cttctcaaga tgtgacgaac ggagaaaaaa tgacaggtca ttaattgcct ccctcgccag
     4321 caaaaccagg ccttcaggat ccactttaag tatctttcgt ccgtcaaatt caatggtact
     4381 gacgaaatct tccgtgagaa gacggtataa agttcggtct tctcctgtct gaaacatttt
     4441 ctcgtaaata aatcctgcca taaaacaata ttttaatgtt cccgggtaaa agggtttaac
     4501 caaatgtaga aattataaag aaggaaaaca ataaaaaagg acagttatct ctcaccggga
     4561 aaaatcacag aagttcttca agatgaaatt tctcattcat ccttgcaccc tttggtgtat
     4621 ctttaagatt acagcattca acaaaaatat catgctgaaa aagaagaata tatccaaatt
     4681 taattgcttc ccggcagaga cgttcttttt cagcgatggt ttttaatgat tcaatgtcgt
     4741 aactcatatt ccacaataat ggtatatggg ctgctgttgg aataaggtcg ccggtatata
     4801 ctattgtcct gtccttatat ttaattattg gtatcattag ccccggagta tgaccatagc
     4861 aaaaaattac agaaacaccc ggaaaaagct ctccttcatt atcaacaaga ttcagatgtc
     4921 cgttttgcgc cagtggtaaa aagttttctt ccggataggc atcttcttcc ctcggattac
     4981 cttttactgc ccattcccac tgtgccctgc tcacatgata tgttgcctcc ggaaaggtga
     5041 gctcatagct tccctgcctt gggccgtttc ttaccgctcc tccgcagtga tcagcatgaa
     5101 ggtgtgtaag aaacacatct gtaatatctt ccggagcaaa ccccctggca gcaagccctt
     5161 cgagtagacc ctccccgtca tgaatatgca gatatctgaa aaaccgttca tcctgtttgt
     5221 tgccccatcc ggtgtcaaca agtattacac ggccgccagt ttctattacc aaagaacgca
     5281 atgccagtgt tacaagatta ttctcatcgg caggataagc cttcgaccat aaagctttgg
     5341 gaattactcc gaacatggcc ccgccatcaa ctttaaaatt tgaaatatta aaaagatgta
     5401 acttcatgtg agcaataatt ttcttaagtt ctttacagca ctttgataat acttatgtta
     5461 ttgatcatta gggattaaaa acaatgttta tatgccatat ccgatttatc ctcatgtcat
     5521 ataattttca ggttcccatt tgcctgtttg gtattccaat tttaacaatt caaagtttgc
     5581 aaaaaatata aatacctttg aaaaaagaaa acattaaaat caggcatatg aaaaatactg
     5641 caattttgct ggcggtaatt tttaccgtgc agtgcatcaa tgcccagacg ggcaaggtta
     5701 tggataacct tgtaatgaaa agcaaaattt taaacatgga caggaaatat gctgtttacc
     5761 tgcctcctga ttatgagaca tcacaaagaa gctatccggt gctttatctt cttcacggtg
     5821 caggtgacga ccagaccggt tgggtgcaat tcggagaggt tctccatatt gcagatgaag
     5881 ccatcaggaa cggaaaggca acccccatgg tcattatcat gcccgatgca aataccggaa
     5941 gaagaggcta tgtgaatgat attaaaaatg aatggagata cgaagatttc ttctttgaag
     6001 agttcatgcc ttatgtcgaa aaaacttacc ggataaaaag cgaaaaaagg tacagggcaa
     6061 ttgccggatt atcaatggga ggggaaggca ctttcatata tgcactgcat catcctgagc
     6121 tattttcatc tgcatgccct ctcagcgctg caacaggtcc ccgcaatgtc gaggaaatgc
     6181 aaaactacag gctctggggt ttggctgaca atgttactca ggaacagaaa gaagcatact
     6241 tcaaaaaata tagcaccctg tacctcatcg aaaatatgcc cgacaatcag aaacgtgcag
     6301 tcagatggta tatcgactgt ggtgatgatg acttcctcta tgaagggaac tccctcgtgc
     6361 acatagccat gcgcaaaaag gaaatacctc atgaattcag aatccgcgac ggaggacaca
     6421 cctggaccta ctggagaagc gccttaccaa cagtactcga atttgtttca atgagctttc
     6481 accagttctg agcaatgaaa caggttctgt ttgcactctt tttaataatc gccgtttcct
     6541 tacatacaga ggcacaattt gttacggagt tcattcctcc ggtaagagtg gtcaaagtgg
     6601 cagaagatgc atggttcgct gatttcggaa aagacgcctt cggaacactc gtcctgaacc
     6661 tgaaagtcaa actgcccgac acacttatca ttcgccttgg agaaaaactt acagaaccct
     6721 gcaaaataga cagaaagccc gggggatcag tcagatacgc cgaagttaag cttccggttg
     6781 atccatcccg tgaaaagtat atcatcaaat tgccggcaga taaacgtaat acttcacctc
     6841 ctgcagttgc cctgcccgat accttcggtg ttattatgcc cttcaggtac tgtgaaatag
     6901 aaaattacag cggggtaatt acaggagaca atatcagaca aaaggcatat ttctggaaat
     6961 tcaaccctga agccagcgct ttctcatcat ccgacacaat cctgaacagg gtatgggatt
     7021 tgtgcaaata ttccatcaag gctaccagtt ttgccggcct ttatattgat ggcgaccgcg
     7081 agagaattcc ttatgaagct gatgcataca ttaaccagtt gagccattat gcagtcgata
     7141 gcgaatatac tcttgcccgg agaacaatgg aacatttcat taataatccc acctggccta
     7201 ctgaatggat tttgcataca gtaatgatat tctaccagga ttatatgtac acaggagaca
     7261 tttccctctt ttcaaaatat tatgagcaac ttaaagccaa aacacttgtt gaccttgcac
     7321 gcgacgatgg tttgataagt tcaaaatcgc caaagcttaa cggtgaaatg atgaggaggc
     7381 ttggcttcag ggatacaact cagcgtattc gcgatattgt cgactggcct ccggcacaaa
     7441 aggatacagg ttggaaactt gcaactcctg agggtgagcg cgatggttat gacatgaagg
     7501 acataaatac tgtcgtaaac tgcttccatt atatcaatct tgaaataatg tcagaaattg
     7561 ctggcaggct tggcaaaaaa gaagattctt tatggtttgc aaaccgtgct gcacttgtga
     7621 agacttccat taacaacaaa cttttcgaca gcaggaaagg tatttatatc gacggtgaag
     7681 gctctgaaca ctcatcaatc catgcaaata tgcttccact tgcttttgga atagtcccgg
     7741 aaaatgcaat taattcagta ctaaatttca ttaagtcgag aggtatggca tgcagtgttt
     7801 acggggccca gtatcttctt gaaggtcttt acaatgcagg tgaagccgat tatgctctaa
     7861 gccttctgac ctctacaacc gaccgtagct ggtggaacat gataagatca ggctcaacaa
     7921 taaccatgga agcatgggac atgaaatata agcccaactc cgactggaat catgcatggg
     7981 gttcagcacc agcaaatatc attgtacgat atctctgggg aataaaaccc ctcgaacctg
     8041 gcttcgccag ggcagaaata aagcctcagc ccggaagcct gaagtggtca acaataaccg
     8101 ctccaacaat aaaaggccag ataaaagcat cattcagaaa gaagagaggt tccgaaaaat
     8161 atttgattga tattcccgaa gggatgaagg ctgaatttgt acctccacca tccctctaca
     8221 aatggatagt tttgaacggc aaaaaatatg acgccacaat aaacagaata tcacttaaga
     8281 ctggtaaaaa cattattatt ctgaaacaat aatccctgat gagaattcat tttatcgcta
     8341 taggcggagc agttatgcat aatctggcaa tcgcgctgca caggaacgga catatggtga
     8401 ctggctctga tgacgagatt tacgaaccct cccgctcaaa ccttgcagca tacgggcttc
     8461 ttcctccatc agcaggatgg ttccccgaca agattcattc aggtctggat gctgtaatac
     8521 tcggcatgca tgcaaggtct gacaatccgg aactgaaaag ggcacaggaa ctcggaatta
     8581 aaattatgtc ctttccggaa ttcctttatg accagacaag aaacaagaaa agaattgttg
     8641 ttgcaggcag ccacggcaaa acaacagtca catcgatgat aatgcacgta tttcgccatg
     8701 ccggaatgaa attcagttat atggcaggtt catccctgaa aggtttcgac cttatggtcg
     8761 gcctttctga cgactctgaa atagcagttt ttgaaggtga tgaatatcct gcttcagctc
     8821 tcgataaacg gcctaaattt ctactttata agcctgatat tgctatactt aacggtattt
     8881 catgggatca catgaatgtt tacaaaactg aagaagaata cattcatcag ttcagaattt
     8941 ttgcagaatc agttagtgaa ggaggaacac ttatttattt caaggatgac cctctggtta
     9001 accggatagc ctcagaagtg aagggtgata ttaataaaat cccctacagg gtgcatggat
     9061 attttataaa taaaataggt gttttcggtg caacacataa tagggtcatt ccacttaaat
     9121 ttttcggtga acacaatatg cagaatcttt ctgcagccag agaagcatgt tttcaggcag
     9181 gaataaccga agaccagttt tatgaagcta ttcagtcatt tgaaggagct gccggaaggc
     9241 ttcagatcct gaaaaaatct gacaggggaa ttgtctttta cgatttcgct cattcaccct
     9301 caaaggtaaa agcaaccata gaggcagttg cagagacatt tgcaggaaaa agaatcgttg
     9361 catgtcttga gttgcacacg tattccagcc tgaatgctgg atttctgccc caatacagaa
     9421 acacaatgga cagggcatcg gtgccttatg tgtttttcag tcctcatgct gtggaactaa
     9481 aaaagcttcc tccacttaat ccggatataa ttactggcgc gttttgcaat aatagtttaa
     9541 gggttttcag taatgccgat gaccttttca gtgatattct cagagaaaag cagaatgata
     9601 caatatatct ttttatgagt tccggtaatt tcgacggcta caacctaaaa aagctggcag
     9661 atgaactggt gtaatctata atgttgcata atgattctgt aaattatatt tgaattacta
     9721 tctttgcccc gcaaaatcaa acaaggcaga catgaaaaaa atttttgtat ttgttgtttt
     9781 tattgcgtta atatcttttt caacatttgc tcaggaatac aagaccagtc tgggtctcag
     9841 agccggctgg ccttatgggt tgacagttaa gcatttcata agcagaacca gtgctcttga
     9901 aggaatactt gcaagcagct ggcacggctt tgttgttaca ggactttatg aaaatgaaaa
     9961 ctggacaggg agctatcctg gacttaactg gttctggggt gttggggcac atgtcggttt
    10021 ctgggatgac aatccgcacg taaatactac aggttctgtg ataggtctcg acggcattct
    10081 tggactcgag tacacttttg acgaaatacc tctgaacctc tctctcgata tattaccatc
    10141 attcaacatt tttggtgcta caggatgggg aggaattggc ggtgcactgt cgatcagata
    10201 tgtattttag catttttatt taaattgatg caagtaaaaa attgaaattt ttcctgttaa
    10261 aactttataa aaaattttga agataggaaa aaagatattt tctttgcatc cgcatttttg
    10321 cgttcttttt atggtccatt cgtctagtgg ttaggacgtc aggttttcat cctggtaaca
    10381 ggggt
//
LOCUS       DAOSNB010000051        10361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6446_length_10361_cov_3.958665, whole genome shotgun sequence.
ACCESSION   DAOSNB010000051 DAOSNB010000000
VERSION     DAOSNB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_10361_cov_3.958665"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PLG42_03595"
     CDS             complement(<1..1501)
                     /locus_tag="PLG42_03595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU30328.1"
                     /translation="MELSVIIVNYNVRYFLEQCLNSVTRASEGISCEIFVVDNNSEDD
                     SCKMVSERFPEVKLIRNSHNAGYAKACNQAMAVASGEFVLLLNPDTIVGGNTFRRCID
                     FMRSHEEAGALGVKMIDGNGRYLKESKRALPVPLTAFFKMTGFSRLFPRSPLFSKYYM
                     AHIDENETSEIEILTGAFMFIRKKVLEKTGFFDENFFMYGEDIDLSYRILKEGYKIYY
                     FPEEKIIHFKGESARKIPVNSIIYFYKAMLVFAGKHFRKKDVILFHLLIKPAIYSRAA
                     LSLLNKVFVILLPLISDIAAISAGVLASAYIWGAQVHGLNSDFSPLPTAGLLALFILP
                     GLISIFLAGGYRKPTYIIQVIKGLIPGCIVILALYAIIHWKTGYTAVTMLMGCFAVLI
                     TVPLFRIILALAGFKDLNNPFITVKNAVVAGSRESYFRICDLVKETDKSVNIKGRVSL
                     LKNDPGDETVGNIDQLQEILNTHRIKVIFFSLRDILPSQLISIIEKMAAF"
     gene            complement(1564..2184)
                     /gene="recR"
                     /locus_tag="PLG42_03600"
     CDS             complement(1564..2184)
                     /gene="recR"
                     /locus_tag="PLG42_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOU30329.1"
                     /translation="MGITVFPSRLLENAVNEFASLPGIGRKTALRLVMHLLRKDSEEV
                     RRFGETIIRLREDIKYCKICHSISDTEICGICSDKSRDPSIICVVESVQDVLAIENTR
                     QYRGLYHVLGGIISPIDGIGPSDLNIDSLEEKVKKGGIVEVIFALSTTMEGDTTSFYL
                     YKRLNKYNLKLSVLSRGVAIGDELEYTDEITLGKSIINRTPFINPA"
     gene            2292..3752
                     /locus_tag="PLG42_03605"
     CDS             2292..3752
                     /locus_tag="PLG42_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU30330.1"
                     /translation="MSPFIILTVFLVYTAILFVVTYLTARKADSHTFYLGKNISPWYV
                     VAYGMIGASLSGVTFMSVPGWVKDTQFSYMVVVFGYLFGYFVIALVLLPLYYRLKLTS
                     IYTYLEKRFGFWSYKTGAGFFILSRTLGASLRMFLVINVLQVFVFDAWKVPFWVNVLI
                     FITLIILYTFKGGIRTIVWTDTLQTTFMLLAVVLSVLYISKDLGKPVGQLVTLIREQG
                     ITKMFITDWHHERFFLKQFFSGMFITIVMTGLDQEMMQKNLSCKNIRDAQKNMFTFSG
                     ILVFVNLLFLFLGAILLVYANTMNIQTKMTDEIFPTIALKYLSPVAGLVFLIGLISAA
                     FPSADGALTSLTTSFTIDFLGLHRRQDLTEKARTRIRYLVHISIATVFFVAILVFRAV
                     NNRAVIDNLFTIAGYTYGPLLGLYSFGLFTRRDVNDRATPFIAIISPVVCYFLSHYSV
                     QLFNGYRFGFELLLLNGFLTFSGLWAFSRKSNRKAE"
     gene            complement(3753..4454)
                     /locus_tag="PLG42_03610"
     CDS             complement(3753..4454)
                     /locus_tag="PLG42_03610"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOU30331.1"
                     /translation="MKKICVFFIEIIITFFLVIAFTTIASGQDTVAVVFPLKINIGAD
                     IYGPAYYYAEKNSLTIEGFISVDRDTMKSYSFEAGYSRFNYEQYNYSYKCKGFFIRAG
                     IDFNFISPKVSKGRYYAGTGLKYGASLYSHEIPFFESENFWGKLSQSIGKSTHIAHFI
                     EASPGIRTELFKNFSIGWTIRLRILLYGGTGKDLKPIYIPGYGNGTKSFSPGINYYFV
                     WSIPYKKVTLKPAGK"
     gene            complement(4426..4890)
                     /locus_tag="PLG42_03615"
     CDS             complement(4426..4890)
                     /locus_tag="PLG42_03615"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOU30332.1"
                     /translation="MKNRIILYCSIFLAISAMMISCVPQTCNEKTESLAGATFYKTGT
                     GKIEAPDTVSIFCLKEDISKFYQKLLKRAIIYLPFDPSAEKCTFILRINQTTDTVAFN
                     YTTFPYLVSKECGYTYCFSVTGYWSTKNIIDTIIVVNNRITTGNEENMRIFY"
     gene            complement(4949..6793)
                     /locus_tag="PLG42_03620"
     CDS             complement(4949..6793)
                     /locus_tag="PLG42_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOU30333.1"
                     /translation="MEVTRTFDIIELNLKQYPRDDMYGGRNDGGWYTYSTEDVANHVN
                     WVSYGLMSMGYKQGDKIATISPNKPEWNFVDMGLAQAGMIHVPVYPTIGTDEYAYILD
                     HAEVKAIIIGNKSIYNKVKPIADNLKNLKDIFSFEPVEGVRDFSEIIEAGKSNADKYA
                     DELKRIKDSIRPEDMVTIIYTSGTTGNSKGVMLSHNNLISNVKATVGAHHMGYGHKAV
                     SFLPLCHVYERMMNYHFQYKGISIYYIDNMGIIAECIREVKPNLFTTVPRLLEKIYDN
                     IITKGKSLPLIKRLIFFWAVNLGLKYKLKGKSAFYKFKLSIARKLVFSKWREALGNNV
                     EAIVSGGAALQNRLERIFWAAGLPVLQGYGLTETSPVIAVNPLRLDDIKFETVGPVVE
                     NVEVKIADDGEILCKGPNVMLGYYKAPELTAEVIDSEGFFHTGDIGVIEDGRFLRITD
                     RKKEMFKLSAGKYIAPQPIENKLKESMFIEQAMVIGENEKFASAIISPNFTYLHDWCS
                     EHKIHFRDNKELIEIPEVIDRIAKEVNHVNKSLGEHEQIKRFRLVTDEWSPQTGELSP
                     TLKLRRNIIAEKYKDIIADIYMRSDKEQGVINRIKNGISSILKGLPKF"
     gene            complement(6976..9609)
                     /gene="alaS"
                     /locus_tag="PLG42_03625"
     CDS             complement(6976..9609)
                     /gene="alaS"
                     /locus_tag="PLG42_03625"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOU30334.1"
                     /translation="MKSGELRQKFLDFFKSKGHVIVPSASMVVKNDPTLMFTNAGMNQ
                     FKDIFLGHQQAVHRRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEFLTAWLKIPEERLYATVFEGSESENVPFDEESYRYWLKCFNNASNKVLK
                     GSKKDNFWEMGDTGPCGPCSEIHIDLRSENERKEMPGHLLINKGHPLVIEIWNLVFIQ
                     YNRKADGTLEPLPEKHVDTGMGFERLCMVVQGKTSNYDTDIFRSIIDEISSVTGKKYG
                     HGGMQDVAMRVIADHLRAVSFSIADGQLPSNNKAGYVIRRILRRAVRYGYTYLGMEEP
                     FIHRLTGTLASIMGDQYPELIKNKDLITRVIQEEEAAFLKTLGKGLRMIEKRISELKE
                     EGKDTLPGNEAFELYDTYGFPVDLTELILRENQLKIDTEGFEAAMNAQKERSREDAAV
                     DTGDWVIVRESSDTTEFTGYEKTEDDVLITRYRKVKIKGKDQYNLVFDRTPFYAESGG
                     QAGDSGILTYGDEKIKITDTIKENNLIIHITDRLPSDPSAVFRASVDTRKRLMTACNH
                     TATHLLHHALREVLGKHVEQKGSLVTWERLRFDFSHFAKMTQEEIIKVEKIVNQYIRQ
                     NIDQTVYKDVPFEEAKKIGAIALFGEKYGDTVRVVKFGESVELCGGTHVRNTGSIGIF
                     KIISESAISAGVRRIEAVTGEKAEEYINSRISEADEVAELLKSTGNIAENVRKLLSEN
                     AALRKNIERMQSQLAAIYRKNLEDKATVVNGIRFIKGIVDTDSPELLKNIAHQLRNIY
                     DDLVFVAGSSSDKKAHLIVLVSDRLVKEKNISATDLIKYISPAIEGGGGGQPFLATAG
                     GKKPSGLQDALNLAENWIKNR"
     gene            9813..>10361
                     /locus_tag="PLG42_03630"
     CDS             9813..>10361
                     /locus_tag="PLG42_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30335.1"
                     /translation="MWFVLSIAVSSVYLKIFFNRFGSPEEKMLNNEIENLKLSYSILE
                     MKFDNAFRVIEQLRLSDDGRYRPILNMDTIPSSFRNPGYGGVDRYNEFKKLKNAELVI
                     NARSKFDLLKNMTKVQEESFREIEERKNEWKRENEHLPKICPVDVSIPRGDGIKFRRE
                     HPVLGTARWHYGQDFLAPYGTEV"
BASE COUNT         2904 a         2160 c         1876 g         3421 t
ORIGIN      
        1 taaatgcggc cattttttca ataatggaaa taagctgaga aggaaggata tctctcagtg
       61 aaaaaaatat tactttgatc cggtgtgtgt ttaatatttc ctgtagttga tcaatatttc
      121 caactgtttc atctcccgga tcatttttta acagagaaac ccttcctttg atattaactg
      181 atttatctgt ttctttaacc agatcacata ttctgaaata actctccctg ctgcctgcaa
      241 ccactgcgtt ttttacagta ataaatggat tattcagatc tttgaaacct gccaatgcca
      301 gaattattct aaatagcgga acggttatca agacagcaaa acatcccatt agcatagtta
      361 cggctgtata tccagttttc caatggataa tggcatacaa agcgaggata acaatacatc
      421 ccgggataag tccctttata acttgtataa tatatgtagg ctttctgtaa cctcctgcca
      481 ggaaaatcga tataagtcct ggaaggataa acagagccag caaacctgca gtgggaagtg
      541 gtgaaaaatc ggaatttaat ccgtgcacct gcgctcccca aatgtaagct gatgccagta
      601 cacctgcaga tatagctgca atatccgaaa ttaaaggcag aaggataacg aaaactttgt
      661 taagaagtga aagtgctgct ctgctgtata ttgccggttt tataagaagg tgaaacagta
      721 ttacatcctt tttcctaaaa tgctttccgg caaaaaccag cattgcctta taaaagtata
      781 tgatgctgtt tacaggtatc ttacgggcac tttctccttt gaaatgaatt attttttctt
      841 caggaaaata ataaatttta tatccttctt tcagtattcg atatgacaga tcaatatctt
      901 caccgtacat gaaaaaattc tcatcgaaaa atcctgtctt ttcaagaacc tttttcctga
      961 taaacataaa tgccccagta agtatttcaa tttcagatgt ttcattctcg tcaatatgag
     1021 ccatgtaata tttgctgaac agaggtgacc gggggaaaag ccttgaaaaa cctgtcattt
     1081 tgaaaaatgc tgtgagtgga acaggaagag ccctttttga ttccttaaga tatcttccgt
     1141 tcccgtcaat cattttaacg cctaatgctc ctgcttcttc atgactcctc ataaaatcaa
     1201 tgcatctcct gaaagtgttc ccaccaacaa tagtatcggg attcagcagc aggacaaatt
     1261 caccggaagc gactgccatt gcctgattgc atgctttcgc ataccctgca ttatgtgaat
     1321 ttcttataag ctttacctca ggaaaacgtt cactaaccat cttgcaggaa tcatcttcag
     1381 aattattgtc aacaacaaat atctcacagg agattccttc agaagccctt gtaacagagt
     1441 ttaaacactg ttccagaaaa tatctaacat tgtagttgac aataattacc gatagctcca
     1501 tataaatatg gtctgaactc taaaatttac ggttatttta aaattctata ttttactata
     1561 agtttatgca ggattaataa aaggtgttct gttgattatt gattttccga gtgttatttc
     1621 atcagtgtat tcaagttcat cgcctattgc cacccctctt gagagaaccg ataattttaa
     1681 attatacttg ttaagcctct tgtacaggta gaaactggtt gtatctcctt ccatggtggt
     1741 actaagtgca aaaattactt ccacaatacc gccctttttg actttttctt cgagtgaatc
     1801 aatgttcagg tcggaggggc ctatgccgtc aattggtgag ataattcctc caaggacgtg
     1861 atataaccct ctgtattgcc ttgtgttttc tattgccaga acatcctgaa ccgattccac
     1921 cacacatata atcgaaggat cccgcgactt gtcgctgcat attccgcata tttccgtgtc
     1981 ggaaatgctg tggcatattt tgcaatattt tatgtcctcc ctcagtctga taattgtttc
     2041 cccgaatctc ctcacttctt ctgaatcttt gcgaagcaga tgcataacaa gtcttaatgc
     2101 cgtttttcgc cctatgcccg gaagtgaggc aaattcattt acagcattct cgagaagcct
     2161 tgagggaaaa accgtaatac ccattaaatt ttttttcaaa aataattcaa atatgctgat
     2221 attttacctt aaaattttct gacataattt ctttttgcta atcttgtacg gtttttttac
     2281 gtaatttttt catgtcacct tttattattc ttacagtttt tcttgtttat acagctattc
     2341 tttttgtagt aacttatctt actgcccgga aagccgacag tcatactttt tatctgggta
     2401 aaaacatatc tccctggtat gtggtagctt atggaatgat aggggcttcc ctctcaggag
     2461 taacttttat gtcggtaccc gggtgggtaa aggatactca gttcagttat atggtggttg
     2521 ttttcggcta cctgtttgga tattttgtaa tagcactcgt tttgcttcct ttgtattaca
     2581 ggctgaaact tacatcaata tatacatacc ttgagaaaag gtttggtttc tggtcctata
     2641 aaaccggtgc aggctttttt attctttcaa gaacactcgg agcttctttg agaatgtttc
     2701 tggttatcaa tgtgttgcag gtttttgtgt tcgatgcatg gaaagttccg ttctgggtta
     2761 atgtgctgat ttttataaca ctgattatac tttacacttt taaaggagga ataagaacca
     2821 ttgtctggac cgacactctt cagacaacct tcatgctcct tgcagtggtt ctttcggttc
     2881 tttatataag caaagacctg ggaaaacctg ttggacagct ggttactctg ataagagagc
     2941 aaggcattac aaagatgttt ataaccgact ggcatcatga aaggttcttt ctcaagcaat
     3001 tcttcagcgg aatgtttata acaattgtga tgaccggtct tgaccaggaa atgatgcaga
     3061 agaatctgag ctgtaaaaat ataagggatg cccagaagaa tatgttcact ttcagcggaa
     3121 tactggtttt tgtaaacctt ctgttccttt tccttggggc aattctgctg gtatatgcaa
     3181 atacaatgaa cattcaaact aaaatgactg atgaaatttt ccctactatt gcacttaaat
     3241 acctgagtcc ggttgcaggt cttgtatttc taatcggact aatatctgca gctttcccga
     3301 gcgccgatgg tgcacttacc tccctgacca cctcctttac cattgatttt ctggggctac
     3361 atcgccgcca ggatcttaca gaaaaagcca gaacaagaat acgttatctg gttcacatct
     3421 caattgcaac ggtatttttc gtggcaatcc ttgtattcag ggctgtaaac aacagggccg
     3481 ttatagataa cctttttaca attgcaggat atacatatgg tccacttctg ggattatatt
     3541 cttttggcct ctttacaaga agggatgtta acgacagggc aacacctttt atagcaataa
     3601 tttctccggt cgtctgctac tttctgagcc actattcagt gcaattgttt aacgggtaca
     3661 ggtttggatt tgaacttctt cttctcaatg gctttctcac tttctccggg ctctgggctt
     3721 tcagcaggaa aagcaacaga aaggcagaat aattatttac cggcaggttt tagagtaact
     3781 ttcttataag gaatactcca gacaaaatag taatttattc cagggctgaa tgattttgtt
     3841 ccattcccat accccggaat atatataggt ttcaaatctt ttcctgttcc gccgtaaaga
     3901 agaattctca gccttatcgt ccatcctatt gaaaaatttt taaagagttc tgtacgtatt
     3961 ccaggtgagg cttctataaa atgtgcaata tgcgtggatt taccgattga ctggctcaat
     4021 tttccccaga agttttcact ttcaaaaaac ggaatttcat gagaataaag gcttgcccca
     4081 tatttcagac cggtaccggc atagtacctg cctttcgaaa cttttggcga aataaagttg
     4141 aagtctatac ctgcccttat aaaaaatcct ttgcatttat atgagtaatt atattgctca
     4201 taattgaatc tactgtaacc agcttcaaat gaataagact tcatagtatc cctgtcaact
     4261 gatataaaac cttcaatagt gagagaattt ttctccgcat aatagtaagc tggtccgtaa
     4321 atatctgcac ctatatttat ttttaaggga aaaacaacag caacagtgtc ctggcctgat
     4381 gcaattgtgg tgaaggctat caccagaaaa aatgttataa taatctcaat aaaaaatacg
     4441 catattttct tcatttccgg tagtaattct gttgtttacc acaattattg tgtcaataat
     4501 gttttttgtc gaccagtaac ctgtaactga aaaacaatac gtataaccac actctttaga
     4561 gaccaggtaa ggaaatgttg tgtagttgaa agctacggtg tcagttgttt gatttattct
     4621 tagaatgaac gtgcattttt ctgcagatgg atcaaaaggg agataaataa ttgctctttt
     4681 aagcagtttc tggtaaaact tagaaatatc ctctttaagg caaaatatgg aaacagtatc
     4741 cggggcttcg atttttccgg ttcctgtttt gtagaatgtt gctcctgcga gtgattctgt
     4801 tttttcattg cacgtttgag gcacgcatga aatcatcatt gcagaaatcg caagaaaaat
     4861 tgaacaatat aagataatgc ggtttttcat atttagctca gaagctcaaa aatataaaat
     4921 tttgctttga gcctccggca aaatgcatct aaaatttcgg aagtccttta agaatactgc
     4981 tgattccgtt tttaatacga tttattaccc cttgttcttt atcacttcgc atatagatgt
     5041 cagcaataat atctttgtat ttttctgcaa ttatgtttct tctcagtttt aatgtggggg
     5101 agagttctcc agtctgcggc gaccattcat cagtaacaag cctgaatctt tttatctgtt
     5161 catgttcgcc gagactttta ttaacatggt taacttcttt tgcaattcta tctattactt
     5221 ccggaatttc tattaattcc ttgttatccc tgaagtgaat tttatgctcc gaacaccagt
     5281 cgtgcaggta tgtgaagttt ggtgagatga ttgcactggc aaatttttcg ttttctccga
     5341 ttaccattgc ctgttcgata aacattgatt ctttaagttt gttttcgatt ggctgaggcg
     5401 ctatgtattt acctgccgag agtttaaaca tctctttttt acggtcggtt atacggagaa
     5461 aacgcccgtc ttcaattaca cctatatctc ctgtgtggaa aaatccttca ctgtcaatca
     5521 cttcggctgt cagttcaggt gctttgtaat atcccagcat tacattgggg cccttgcata
     5581 gtatttcacc gtcgtcggca atttttactt caacattttc taccacaggt ccaacggttt
     5641 caaattttat atcatccagc cgtaaagggt tgacagctat aacgggagag gtttcggtga
     5701 ggccgtaacc ctgcagcaca ggcagtccgg ctgcccagaa aatacgctcg aggcggttct
     5761 gtaatgctgc cccacctgat actattgctt ccacattatt gcccagcgct tccctccatt
     5821 tgctgaaaac aagtttacgt gctatgctta acttaaattt gtaaaaggct gatttaccct
     5881 tcagtttata ttttaatccc aggtttaccg cccagaagaa aataagtctt ttaattaaag
     5941 gcaagctctt gcctttggta ataatgttat catatatttt ttcaagcaac ctcggaactg
     6001 ttgtaaaaag attaggtttt acctctctga tacattcggc aataataccc atattgtcaa
     6061 tataataaat gcttatgccc ttatactgaa aatggtagtt catcattctt tcgtaaacat
     6121 ggcataacgg cagaaaactg acagctttgt gtccataacc catgtgatga gctcctacag
     6181 ttgccttgac attggatatc agattattat gtgaaagcat cacacctttc gaatttcccg
     6241 tagtgcccga cgtataaatt attgtaacca tgtcttcagg ccggatacta tccttgattc
     6301 tcttcaattc atcagcatat ttgtcagcat tagatttacc tgcttctatt atttccgaaa
     6361 agtctcttac accttcaacg ggttcgaatg aaaaaatatc tttgagattc tttaagttgt
     6421 ccgcaattgg tttaaccttg ttgtatattg acttattgcc tataataata gcttttactt
     6481 cagcatggtc gagaatatat gcgtattcgt cggttccaat tgtaggatag accggtacat
     6541 gaatcattcc agcctgagca agtcccatat cgacaaaatt ccattcaggc ttgtttggag
     6601 atattgtggc aatcttgtct ccctgtttgt aacccattga catgagtcca taacttaccc
     6661 agtttacatg atttgctaca tcttcggttg aatatgtata ccagccccca tcattcctgc
     6721 ctccatacat gtcatccctg ggatattgct tcagattaag ctcaatgata tcaaatgttc
     6781 tggttacttc cattttgctt cattattaaa tgaaatgcaa ttctaataaa atttttcgaa
     6841 caaatacaaa attgtcacaa taatatcaga gataatcata ctaagtaact tattttcaat
     6901 ttaatatacg ataagaaaat aaatgggttt ttcgaccaga agtcttctgt tacggaaaag
     6961 ctgaattgac cttgattatc tgtttttaat ccagttttca gcaaggttca gagcatcctg
     7021 aagtcctgac ggtttttttc ctccggcagt tgcaaggaat ggttgtccgc caccgccgcc
     7081 ttcaatggca ggtgaaatat atttaatcag gtcggttgcc gaaatatttt tctctttaac
     7141 aagcctgtca ctaacaagaa ctatcagatg tgccttttta tctgaagatg aaccagctac
     7201 aaataccaga tcatcataaa tgtttctgag ctggtgggca atgtttttca gcaattcggg
     7261 tgagtcggta tccacgatgc cctttatgaa tctaatccca ttaactacag tggctttatc
     7321 ttcaaggttt ttccggtaga ttgcagcaag ttgcgactgc attctctcga tgttctttct
     7381 tagggcagca ttttccgaaa ggagtttcct tacattctct gcaatgtttc ctgtgctctt
     7441 aagaagttct gccacctcat cagcttcact gattcttgag ttaatatatt cttccgcttt
     7501 ctcaccggtt acggcttcta ttcttctaac tcctgcagat attgcactct cagaaatgat
     7561 tttgaaaatt cctatcgaac ctgtattcct gacatgcgtt ccaccgcaca gctcaaccga
     7621 ctcgccgaac tttacaactc gcacagtgtc gccatatttt tcgccaaaaa gtgcaatggc
     7681 accaattttt ttggcttctt caaaaggaac atctttatag acagtctggt cgatattttg
     7741 tcttatatat tgatttacaa ttttttcaac ctttatgatt tcttcctgag tcatttttgc
     7801 gaagtggctg aaatcgaatc tgaggcgctc ccaggtaacg agtgatcctt tctgttcaac
     7861 atgttttcca agtacttccc ttaatgcatg atgaagcaga tgcgttgctg tatggttgca
     7921 ggctgtcatg agtcgcttcc tggtgtcaac cgaagccctg aacactgcag aaggatcaga
     7981 aggcagccgg tcggttatat gaataatcaa attgttttcc ttaattgtat cagtaatttt
     8041 tattttttcg tccccgtatg tcagaattcc cgagtcacct gcctgcccgc ccgattcggc
     8101 ataaaaaggt gttctgtcaa ataccagatt atactggtct tttcctttaa ttttaacttt
     8161 gcggtatctt gtaatgagaa catcatcttc tgttttctca taacctgtga attcagttgt
     8221 atcagatgat tcccttacaa ttacccagtc gccagtatca actgcagcat cttctctcga
     8281 cctctctttc tgagcattca tagctgcttc aaacccttcc gtatcaattt ttaactgatt
     8341 ctctctgaga atcaattctg tgaggtcaac aggaaatccg taagtgtcgt aaagttcaaa
     8401 tgcctcattt cccggtaatg tatctttgcc ttcctctttg agctctgaaa ttctcttttc
     8461 aatcattcta agtcctttac cgagagtctt gagaaaagca gcctcctcct cctgtataac
     8521 tctggtaatg aggtctttat ttttaataag ctccggatac tgatcaccca ttatactggc
     8581 aagcgtacca gtaagcctgt ggataaaagg ttcctccatg ccaaggtatg tataaccgta
     8641 ccttacagct cttctcagta tgcgacgtat tacataaccg gctttattgt tcgacggaag
     8701 ctgcccatca gctattgaga aagagactgc ccgcaaatgg tcggcaatta cccgcatggc
     8761 aacatcctgc atccctccat ggccatattt tttacctgta actgatgaga tttcatctat
     8821 aattgacctg aaaatatctg tgtcataatt tgaagtttta ccctgaacca ccatgcagag
     8881 gcgttcaaat cccatgcctg tatccacatg cttttcaggc agcggttcaa gagttccatc
     8941 ggcttttctg ttatactgaa tgaaaacaag gttccatatc tctattacca gcggatgtcc
     9001 tttgtttata agcagatgac ccggcatttc ctttctctca ttctcacttc tcaagtcaat
     9061 atgtatttca gaacatggcc cgcatggacc tgtatcgccc atctcccaga aattgtcctt
     9121 ctttgagcct ttgagaactt tgttgctggc attgttgaaa catttcagcc agtagcgata
     9181 cgattcttca tcaaatggaa cattctcact ttcgctaccc tcaaaaacgg tcgcataaag
     9241 cctctcctcg ggaattttaa gccaggcagt gagaaactcc catgcccatt cgatagcttc
     9301 ctttttgaaa taatcaccaa acgaccagtt accgagcatt tcaaacattg tatgatggta
     9361 agtgtcgtgg cctacctctt caaggtcgtt gtgtttgccc gacaccctga gacatttctg
     9421 tgaatttgca accctcctat gaacggcctg ctgatgccct aaaaaaatgt ccttaaactg
     9481 gttcataccc gcattggtga acatgagtgt gggatcattc tttaccacca tacttgccga
     9541 cggcacaata acatgtcctt tggatttgaa aaaatcaaga aacttctgcc tcagttcacc
     9601 ggatttcatt gactcattct tatttaagtg gttaaaaaat tatcccgaat cgggcttgta
     9661 aaattaattt atttacttta gatttgtcag gacctgaata aaaaagatat ttcagtttgt
     9721 ttttaaaaaa ccgtaaatac atttttgatc atagtgacct gcaatttaag caaattaaat
     9781 tgccattgaa aatcagacta ttaagatttt taatgtggtt tgtactttca attgcagtat
     9841 catctgttta tctaaaaatt tttttcaacc ggttcggttc tcctgaagaa aaaatgttaa
     9901 acaacgaaat tgagaatctt aagctttcgt actcaatact ggagatgaag tttgataatg
     9961 cattcagggt gattgaacaa ctgaggcttt ctgacgacgg gcgctacagg ccgatactga
    10021 acatggatac aattccttca tcattcagga atccgggata cggaggtgta gatagataca
    10081 atgaattcaa gaaactgaaa aatgctgagc tggtaattaa cgcccgcagc aagtttgatc
    10141 tgctgaaaaa catgacgaaa gtccaggaag aatcgttcag ggaaattgaa gaaagaaaaa
    10201 atgaatggaa gagggaaaat gagcacctgc cgaaaatatg ccctgttgat gtctcaatac
    10261 ccagaggtga tggaataaaa ttccggcgag aacatcccgt tcttggcact gcaagatggc
    10321 attacggaca ggatttcctt gcaccatatg gtactgaggt g
//
LOCUS       DAOSNB010000052        10288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6502_length_10288_cov_4.092641, whole genome shotgun sequence.
ACCESSION   DAOSNB010000052 DAOSNB010000000
VERSION     DAOSNB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6502_length_10288_cov_4.092641"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLG42_03635"
     CDS             <1..274
                     /locus_tag="PLG42_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOU30336.1"
                     /translation="PALTGRKKGLLISMILHVLTILFVIAAGIVGRSGIIFWAGATIF
                     ILLLIYQHVIVRPHDISRVNLAFATTNGIASIIFAIFVITDLFVKI"
     gene            346..1470
                     /locus_tag="PLG42_03640"
     CDS             346..1470
                     /locus_tag="PLG42_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30337.1"
                     /translation="MNDITIKEVKTRRDLKEFIYLPEEVHKNNKKWLPPIYQDEWDLY
                     NPEKNKSFNHCDTVLYLAYRNGKPAGRVMDIIHRLYNQIHDEKHGRFAFMECYEDQEV
                     VHALLQKIEQWAKEKGMTKVVGPLGFSDKDPQGFQIEGFEYPPFLAAATNAPYLPLMI
                     EKEGYVKKVDLVNYLTPVPDKLPDVYQRVLERFNLLETFEVVEFKSKKELKPYIIPAL
                     ELMNQTYSEIYGYVPLDDQDKKDLADKYLPILDPHFIVMIKAGEEVVAFAVAVPDMSD
                     GIRKAKGKLFPFGFIYILKELKKSKSLLTLLGGIKKEYRDQGLDVILALKLYEAAIKY
                     GMEYCNSHLILETNTKMRAEFERVGGRISKKFRIYQKDLV"
     gene            complement(1493..3295)
                     /locus_tag="PLG42_03645"
     CDS             complement(1493..3295)
                     /locus_tag="PLG42_03645"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOU30338.1"
                     /translation="MKNNKFYLLSAVIVMLFNAMLFPQEIPQKVINGKTMRDSLIRLG
                     YYVKPEQERIEIKLTPEEAINYLEQKYRSDIWKLAEDPLRVAIGQLVFYAKSKPFDSI
                     RVFLERYPFDSVNIPPEKFFVFDSVTLKIPNIGLTEPGRIDSLVKADTLGIKYAEAGT
                     DTIARPEAAVSKPLISMKDTILLIVTDTLSEVKAFADYHPFKVFRSSFTGDSLLAAVK
                     ALTDFIEARDSTIIMFKGVSETNLPVWLNSRSGDMKRFWLRNEYDDSVTVWVGSAGRN
                     TIGLYLEEGIMFRRPSKHSNIYDAQLDIKQVDTRKLQQVNKLYVKPQYWKYRSEAAFV
                     FNQAMLTNWVKGGENSISTAMDITGYADYNNKELKITSNNFARLKYGLIKSGDNPVRK
                     NLDLLETNSKLNHKAFGKFDFSATMLFKTQISKGYNYPNDSVPVSKFFNPAVLTLGIG
                     LDYKPDKTTSINVAPLSYKATFVPDTATIDQTKYGVPKDRRSMHEPGGSVQITNEFKP
                     LKTVTITNRLQLFTNYIHKPQNIDIDWEMIAQVKLNWFTDLRFNTHFIFDDDTKTVVK
                     NKDGSPVLGPDGKPKKSARIQFKELLGLSFVFRF"
     gene            3560..4906
                     /locus_tag="PLG42_03650"
     CDS             3560..4906
                     /locus_tag="PLG42_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOU30339.1"
                     /translation="MKDLTSGPEGKLIFQFAAPMLLGNVFQQLFNIADSIIVGNFIGK
                     EALAAVGASFPVVFILISLVIGLVMGTTVVISQYFGARDMVRVKKAIDTMYISTFFGS
                     ILLTFIGFIFSAPLLRLLKLPEEIMPQTIQYLHIFIGGLILMFGYNGTSAALRGLGDS
                     KTPLYFLIIATVANIILDILFVAFLKMGIAGAAYATVISNGIAFLLAAIYLNKTHKII
                     NVKINGLIFDRDIFFQSLRIGLPNSIQQTIVALGGLVLMGIVNKFGTNVIAAFSVASR
                     LDAIATVPAMSFAQAISAFTGQNIGANKEERIKTGLASTLKMMATVTFFTTLFIILAG
                     KYIISLFTNDGEVIRIGAQYLTIVSLFYILFTLMFIYSGVMRGAGDSFMPMVITLLSQ
                     WIIRIPFAWIMSGILGVTGIWWSIPASWLVGFSLSYFYYKTGRWKKKRVVTYIEEF"
     gene            4938..5813
                     /locus_tag="PLG42_03655"
     CDS             4938..5813
                     /locus_tag="PLG42_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOU30340.1"
                     /translation="MIKSMTGYGKASVETPQKKITVEIKSLNSKQLDINTRLPWILRE
                     KEPEIRNIVGQKLERGKIDLIISYNIFEGEFLPVINRAAVKNYFSQLSEIAGELGINI
                     SDQILSVIMKFPETLNTEKTELPEEEWTLVRNLLDQSIDMLDKYRVEEGCALEKDIRN
                     SINRIIESLSGIEEYEPGRIMKIREKLMALLEENIQPDKIDRNRFEQEIIYYLDKFDI
                     NEEKIRIKQHCYYFLETLSAETSNGKMLNFIAQEIGREINTIGAKANDASIQKLVVTM
                     KDDLEKIKEQLLNVL"
     gene            5846..6415
                     /gene="gmk"
                     /locus_tag="PLG42_03660"
     CDS             5846..6415
                     /gene="gmk"
                     /locus_tag="PLG42_03660"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOU30341.1"
                     /translation="MPEGKAVIISAPSGAGKSTIVKYLLESGLNLEFSVSATTRQPRG
                     EEKNGVDYYFLTVEEFKKKISNGEFVEWEEVYENHFYGTLRSELERIWAKNKHVLFDV
                     DVKGGISLKKIFGDRALSLFIMPPSIEELEARLKKRGTDSPEKIKIRIQKAGIEMQSA
                     GFFDRIVVNDDLEKAKQEAFSIISNFLNG"
     gene            6412..7017
                     /gene="nadD"
                     /locus_tag="PLG42_03665"
     CDS             6412..7017
                     /gene="nadD"
                     /locus_tag="PLG42_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOU30342.1"
                     /translation="MSSNKKIGLFFGSFNPVHLGHTAIAGYMAEYTDLHQIWFVVSPQ
                     NPLKKKETLLPDRDRLYMVELAIEGNDKFRASDIEFRLPAPSYTIDTLTYLKEKYPDY
                     EFCLIMGEDNLYTLHKWKNAEELVKKYPIYVYPRKDSVRPLNPVLDELLKKAEITIVN
                     APLMEISGTFIRQGIREKRDMSYFLHPAVWKYIREMHFYEK"
     gene            complement(7025..7573)
                     /locus_tag="PLG42_03670"
     CDS             complement(7025..7573)
                     /locus_tag="PLG42_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU30343.1"
                     /translation="MTEEILIAGFGGQGILSMGKILAYSAMMQDFEVSWMPSYGPEMR
                     GGTANVTVIISTERISSPILRQFDTAIILNQQSLDKFEKSVKPGGMLIYDGNGITRHP
                     ERKDIEIYRIDAAEEAARMKSPRTFNMIVLGGFLKIKPLIKIENVIEGLRKSLPERYH
                     KMIPDNEKALIRGMEIIRKLNN"
     gene            complement(7590..8399)
                     /locus_tag="PLG42_03675"
     CDS             complement(7590..8399)
                     /locus_tag="PLG42_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU30344.1"
                     /translation="MKVVTGDKDIIQPENLVYKRSSILTDKQFSYCPGCGHGTAHRIL
                     AEVIEEEGLQPVTVGVCPVGCSVLMYDFLDIDMQEAAHGRAPALATAIKRLLPDRFVF
                     TYQGDGDLAAIGTAETLHACNRGENILIIFINNGIYGMTGGQMAPTTIPGMVSTTSPY
                     GRDVKTMGNPLKITELVAMLPGTCYVTRQSVHTPAAVRKTKKAIKKAVEYQRKNLGTC
                     FIEIVSNCPSGWRMTPVEANKWMEENMFKFYQPGDLKVPPEEPEKSKDDNE"
     gene            complement(8406..9467)
                     /locus_tag="PLG42_03680"
     CDS             complement(8406..9467)
                     /locus_tag="PLG42_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOU30345.1"
                     /translation="MEKELKLMKGNEAIAEAAIRAGVDAYFGYPITPQSEILEYLMEA
                     NPYEKTGMVVLQAESEPAAINMVYGGAACGKKVMTTSSSPGISLMQEGISYIAGAELP
                     CLIVNVVRGGPGLGTIQPSQSDYFQACKGGGHGDYHLLVLAPASVQEMADFVELAFDL
                     AFKYRNPVMILSDGAIGQMMEKVYLKPYREREKTLPEWATTGKPPTRERNIITSLNLD
                     PYLHEKHNQKIQAKYREMEKNEVRFEEFQTEDAEYLFVAYGTSARVCQKAVQLARTEG
                     IKAGLIRPITLYPFPSVRINELAGKMKHIISVEMSAGQMVEDVRLAVNGQTEVTHFGR
                     MGGVIPTPEEVLEDLKSKI"
     gene            complement(9472..9696)
                     /locus_tag="PLG42_03685"
     CDS             complement(9472..9696)
                     /locus_tag="PLG42_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU30346.1"
                     /translation="MAKIKGSIVIEVERCKGCELCVVTCPTGTIALATEVNSKGYYYA
                     YPANPDTCNGCTNCAVVCPDGVITVYRKKI"
     gene            complement(9709..>10288)
                     /locus_tag="PLG42_03690"
     CDS             complement(9709..>10288)
                     /locus_tag="PLG42_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU30347.1"
                     /translation="KDYIILISSSKMFSYAGQRIGLMAVSDHLFNRRYPDLLRYFKSD
                     NFGQALIYGALYGLTAGVTHSVQYGLAALLKAVNDGKYRYVEDVKIYGEKAKIMKQLF
                     LSNGFKIVYDKDIDEPIADGFYFTVSYPGMSGSSLLEELLRYGMSAISLRITDSERTE
                     GLRICVSMVHKNQFDELKNRLEKFHMDHPVNN"
BASE COUNT         2997 a         2124 c         2071 g         3096 t
ORIGIN      
        1 tcctgcactc actggaagaa aaaaaggatt attgatttcc atgattctcc acgtgttaac
       61 aatacttttt gttattgcag caggaattgt cggcaggtca ggaatcattt tctgggcagg
      121 tgcgacaatt tttatattgc tgcttattta ccagcatgtc attgtcagac cgcatgacat
      181 aagcagggta aatttagcct ttgccacaac aaatggtatt gccagcataa tatttgccat
      241 ttttgttatt acagatttgt ttgttaaaat ttaatttttc catatttata acaaatgcaa
      301 ttaaaaattt ttattacttg gctgaataat taaaattttt caatcatgaa tgatatcact
      361 atcaaggagg taaagacaag aagagatctg aaagagttta tctatcttcc tgaagaggtg
      421 cacaaaaaca acaaaaaatg gttacctccg atttatcagg atgagtggga tctgtacaat
      481 cctgaaaaaa acaagtcgtt caatcattgt gatacagtat tgtatctggc ataccgtaac
      541 ggtaagcctg caggaagggt aatggatata atacacaggt tgtacaatca gattcatgat
      601 gaaaagcatg gccggtttgc gtttatggaa tgttatgaag accaggaagt tgttcatgca
      661 ctactgcaaa aaattgagca atgggcaaaa gaaaaaggta tgacaaaagt tgtgggtcct
      721 cttggttttt ctgataaaga tccacagggt ttccagattg agggctttga atatccccct
      781 tttcttgcag cagccacaaa cgccccttac ctgcctctga tgatagaaaa ggaaggttat
      841 gtgaaaaagg tggatcttgt aaactatctc acccctgtac cggataaact ccccgatgta
      901 taccagagag tgcttgagag gtttaatctg cttgaaacat ttgaagtagt ggagtttaaa
      961 tcgaaaaaag aattaaaacc ttatattata cctgcactgg agttgatgaa tcagacctac
     1021 agtgagatat acgggtatgt tcctctcgac gaccaggata aaaaagatct tgccgacaaa
     1081 taccttccca tacttgatcc tcatttcatt gtgatgatta aagcaggtga agaagttgtg
     1141 gcctttgccg tagcagttcc tgatatgagt gatggtataa gaaaggcaaa ggggaaactc
     1201 tttccatttg gttttatata tattctgaaa gaacttaaaa agtcaaaaag ccttctgact
     1261 cttttaggag gaataaagaa agagtaccgc gaccagggac tggatgtaat tcttgcactg
     1321 aaactctatg aggcagcaat aaaatacgga atggaatact gcaacagcca tctcattctt
     1381 gaaacaaata caaaaatgag ggctgaattc gagagagtcg gtggccggat ttcgaaaaag
     1441 ttcagaatct accagaaaga cctggtctga atacacctgt tcatagcaac tctcagaacc
     1501 tgaacacaaa agagagaccc agcaattcct tgaattgtat ccgggcactc ttttttggtt
     1561 taccatcagg accaagaaca ggtgaaccat ccttgttttt aacaactgtt tttgtgtcgt
     1621 cgtcaaaaat aaaatgtgta ttgaatctca ggtcggtaaa ccagttaagt tttacctgtg
     1681 caatcatttc ccagtcgata tcaatattct gcggtttatg aatatagttg gtaaagagct
     1741 gcagtctgtt ggtgatggtt actgttttca gtggtttgaa ttcatttgtt atctgcacgc
     1801 ttccacctgg ctcatgcatc gacctgcggt cttttgggac gccgtatttt gtctggtcta
     1861 ttgttgcagt gtcgggaaca aaagtggctt tatacgaaag cggcgcaaca ttaattgatg
     1921 ttgtcttatc aggcttgtaa tccagaccaa tacctaaggt aagcacagcc ggattaaaga
     1981 atttcgaaac aggaacagaa tcgttaggat agttgtaacc ttttgatatc tgagtcttaa
     2041 acagcattgt cgcactgaaa tcgaatttac cgaaagcttt gtgattcagt tttgaatttg
     2101 tttcaagcag gtcgaggttc ttccttaccg gattatcgcc cgactttatc aggccatatt
     2161 ttaacctcgc aaaattgttc gacgtaattt ttagttcttt attgttataa tcggcataac
     2221 cagtaatatc cattgcggtt gagatactgt tctcccctcc cttaacccag ttggttagca
     2281 ttgcctggtt gaaaacgaat gcagcttcgg aacggtattt ccagtactgc ggttttacat
     2341 aaagcttgtt tacctgttgc agtttcctgg tatcaacctg tttaatgtcg agctgagcat
     2401 catatatatt ggaatgtttt gatggtcttc tgaacattat cccttcctcg aggtacagac
     2461 caattgtatt cctgcctgca ctgccaaccc acactgttac agaatcatca tactcattcc
     2521 tgagccagaa acgtttcatg tcgcccgacc tggaattaag ccatacagga agatttgttt
     2581 ctgaaacgcc tttaaacata ataattgtgg aatcgcgagc ctcaataaaa tcagtcagtg
     2641 cctttaccgc tgccagcaat gaatcacctg taaaagaact ccggaaaacc ttaaaaggat
     2701 gataatcagc aaaagccttt acttcactca gggtgtcagt aactatcaaa agtattgtat
     2761 ctttcatgct tattaaaggc tttgaaacag cggcttccgg tctggcaata gtgtcggttc
     2821 cggcttctgc atattttatt ccaagcgtat ctgccttcac aagtgaatcg attctccctg
     2881 gttccgtgag cccgatattc ggaatcttta atgtcaccga atcaaataca aaaaattttt
     2941 ctggcgggat attaaccgaa tcaaacggat atctctcaag aaatactcgt atagaatcaa
     3001 atggtttgct ttttgcataa aatacaagct gtcctattgc cacacgtaaa ggatcttcag
     3061 ccagtttcca gatatccgac ctgtatttct gttccagata attgattgct tcttcaggag
     3121 ttaatttaat ctctatgcgt tcctgttcag gttttacata gtaacccaac cgtatcaggg
     3181 aatcacgcat agtcttgcca ttaatcactt tctgaggaat ttcctgtgga aaaagcatcg
     3241 cattgaaaag cattacaatt actgctgaaa gcaaataaaa cttgttgttc ttcatcggat
     3301 tgtcccctgt ttaatttcca aaaataaatg tttttatatt attcctctca ataatttgat
     3361 aacgttaaaa aacagaattt taatataaaa ataaaaaagt gctcttacac aggactgtat
     3421 tgaaaggctg tgaatttaga atatttggct ggtggatatc atccatgttt tcggttagct
     3481 gcccgactgc tataaaactt catcaatatt ttgagaatgg ctcgtaaata gttttctttg
     3541 cggttcgtca gaatttgaaa tgaaggacct aacatcagga ccggaaggaa aactaatttt
     3601 ccagtttgct gcacccatgc tgctgggtaa cgtttttcag cagttattta acattgccga
     3661 tagcattata gtagggaatt ttatcggcaa agaagcgctg gctgcggtag gtgcatcatt
     3721 tcctgtagta ttcattctaa tttctcttgt tataggtctg gtaatgggaa ccacagtggt
     3781 tatttcacaa tatttcggtg caagggatat ggtgcgggtt aagaaggcaa ttgatacgat
     3841 gtatatttca accttcttcg gttcgatact tctcactttt attggcttta tctttagtgc
     3901 tccactgctc aggttgttaa aactgcccga agagattatg ccccagacaa ttcagtatct
     3961 tcatattttc attggcggac ttatcctgat gtttggttat aacggtacca gtgcagcact
     4021 cagaggcctg ggcgattcaa aaacacctct ctacttcctc atcattgcta ctgtcgcaaa
     4081 tataattctc gacatattat ttgtggcttt tctgaaaatg gggattgccg gtgcagccta
     4141 tgcaacagtg atatctaacg gtattgcatt cctgcttgcc gcaatatatc ttaacaaaac
     4201 tcataaaatt attaatgtaa aaatcaacgg tcttattttc gacagggata tattcttcca
     4261 gagcctgcgt ataggtcttc cgaacagcat acagcagaca atcgttgctc ttggcggact
     4321 cgttcttatg ggaattgtca ataaattcgg caccaatgtt atagctgcat tctctgtagc
     4381 aagcaggctc gatgcaattg ccacggtgcc agctatgtca ttcgctcagg ctatatcggc
     4441 atttaccgga cagaacatcg gagccaacaa ggaggagagg attaaaaccg gactcgcctc
     4501 aactctgaaa atgatggcca cagttacctt cttcacaact ctattcatca ttcttgcagg
     4561 gaaatatatc ataagcctct ttacaaatga tggtgaagtg atcagaatcg gagcacagta
     4621 cctgacaata gtaagcctgt tttatatctt gtttactctg atgttcatat acagcggagt
     4681 catgagggga gctggtgatt cattcatgcc aatggttatc acgcttcttt cacaatggat
     4741 aatacgtatc cccttcgcat ggataatgtc aggaatactt ggagtaaccg gaatatggtg
     4801 gtcaattcct gccagctggc tcgtgggctt ttcattatca tacttttatt acaaaaccgg
     4861 caggtggaaa aagaaaaggg ttgtcacata tatcgaggaa ttttgattta acttagcaga
     4921 agaaaataga atttgccatg ataaaatcaa tgaccggtta cggtaaagca tctgttgaaa
     4981 caccccagaa gaaaataaca gtcgaaatta aatcactcaa ctccaagcaa ctggatatca
     5041 acaccaggct tccatggata ttaagggaaa aagaacccga gataagaaat atcgtaggcc
     5101 agaagcttga aagaggaaag atcgacctga taatatcata caatatcttt gaaggtgaat
     5161 tcctaccggt gattaaccgg gctgccgtaa aaaattattt ttctcaacta tcagaaattg
     5221 ccggtgaact gggaataaat atcagcgacc agattctgtc ggttataatg aaatttcccg
     5281 agacactgaa tacagaaaaa acagaattac ctgaagaaga atggactctc gtgaggaatc
     5341 ttcttgacca atccattgac atgctcgaca agtacagggt ggaagaaggc tgtgcccttg
     5401 aaaaggacat caggaattca attaaccgta ttattgaatc actttcaggc atagaagaat
     5461 atgagccagg gagaataatg aaaatcagag aaaaactaat ggctttgctt gaagaaaaca
     5521 ttcagcccga caagattgac agaaacaggt ttgaacagga aatcatttac tacctcgaca
     5581 aatttgatat taatgaagaa aaaattagga ttaagcagca ttgttattat tttcttgaga
     5641 cactgtcggc tgagacatca aatggaaaga tgcttaattt tatagcacag gagattgggc
     5701 gtgagataaa tactattgga gccaaagcca atgatgcatc aattcagaag ctggtggtca
     5761 caatgaaaga cgatctggaa aaaataaaag aacagcttct aaatgttctt taatgaaata
     5821 atgaacagaa attaagctgc aaaatatgcc agagggtaaa gcagtaatta tttcagcacc
     5881 atcaggcgca ggtaagtcga ccatcgtaaa atatctgctc gagagcggac tgaatctcga
     5941 attttctgtt tcagcaacaa cgaggcagcc tcgtggtgag gaaaaaaatg gagtagatta
     6001 ttattttcta actgttgaag agtttaaaaa gaaaatcagt aatggtgaat ttgttgaatg
     6061 ggaggaggtt tatgagaatc acttctacgg aactctccgg agcgagctgg aaaggatatg
     6121 ggcaaaaaac aaacatgtac ttttcgatgt ggatgtaaaa ggaggtattt cgcttaagaa
     6181 aatattcggc gacagggcgc tgtcactctt cattatgcca ccctcgatag aagaacttga
     6241 agcgaggttg aaaaaacgcg gaaccgattc cccggaaaaa atcaaaatca ggatacagaa
     6301 agcaggcatc gaaatgcaat ctgctggttt tttcgacagg attgttgtta atgacgacct
     6361 tgagaaagca aaacaggagg cattttccat cataagcaat tttttgaatg gatgagcagc
     6421 aataaaaaga tcggattatt tttcggctct ttcaacccgg tacatctagg acatactgca
     6481 atagccggtt atatggcaga atatacagac ctgcatcaga tatggtttgt tgtgagccct
     6541 caaaatccac tgaagaaaaa agagacactg ctgcccgaca gggacaggct ttacatggtt
     6601 gaacttgcaa ttgagggcaa tgataaattc agagcatctg atattgaatt ccggcttcct
     6661 gctccttcct acacaattga cactctcaca tatctgaaag agaaataccc tgattatgaa
     6721 ttctgcctga taatgggtga agataacctt tacactcttc ataaatggaa aaatgcagaa
     6781 gagctggtaa aaaaatatcc catatatgtt tatcccagaa aagactccgt caggccttta
     6841 aatcccgtgc tcgacgaact tcttaaaaag gctgagataa caattgtaaa tgcgcctctg
     6901 atggaaattt caggcacatt catcagacag ggaataagag aaaagagaga tatgtcttac
     6961 tttctgcatc ccgctgtttg gaaatatatc agagagatgc atttttatga aaaatagcct
     7021 gctgtcagtt attgagcttc cggattattt ccattcccct tatcagagcc ttctcattat
     7081 caggtatcat tttatgataa cgctcaggca gtgattttct gagtccttca atcacatttt
     7141 caatttttat caaaggtttt attttcagaa atccccccag aacaatcata ttgaatgtcc
     7201 tggggctctt catccttgca gcttcctcag cggcatctat cctgtatatt tcaatatcct
     7261 tccgttcggg atgtctggtt attccattgc catcgtatat gagcataccc ccgggtttga
     7321 cagatttttc aaatttgtcg agtgattgct ggttaagtat gattgcagtg tcgaactgcc
     7381 tcagtatggg tgagcttatt ctttctgtac tgattataac tgtgacgttt gctgttcctc
     7441 ctctcatttc aggcccgtac gaaggcatcc agcttacttc aaagtcctgc atcatagctg
     7501 aatatgccaa tattttaccc atcgacagga tgccctgccc accaaaacct gcaataagta
     7561 tttcctcggt catataagta gtttatttat tactcattat catcctttga tttttctggt
     7621 tcctcgggag gtactttcag atcgcccggc tgataaaact taaacatatt ttcctccatc
     7681 catttgtttg cctctaccgg agtcattctc caacctgagg gacagttaga tactatttcg
     7741 ataaaacatg ttcccaaatt ctttcgctgg tactcaactg ccttttttat tgctttcttt
     7801 gttttcctca ctgcagcggg tgtatggacg ctctgccggg taacatagca agtgccaggc
     7861 aacatagcaa cgagttcggt aattttcaag ggattaccca ttgtcttcac atctcttccg
     7921 tagggtgaag tggttgaaac cattccagga atggtggtag gtgccatctg acctcctgtc
     7981 ataccgtaga ttccattatt gatgaatatt atcagtatgt tttcccccct gttgcatgca
     8041 tggagtgttt cagccgttcc tattgctgca aggtcgccgt cgccctggta tgtaaataca
     8101 aacctgtcgg gcagaagacg ttttattgca gttgcaagtg ccggcgcgcg gccatgtgca
     8161 gcctcctgca tatctatatc aaggaaatcg tacataagaa ccgaacagcc tacagggcat
     8221 acaccaacag ttaccggttg caagccttcc tcctctatca cttcggcaag tatcctgtgg
     8281 gctgtgccat gcccgcatcc cggacaatag gaaaactgct tgtcagtcag gatactggac
     8341 cttttataaa ccagattttc tggctgtata atatctttat cacccgtaac taccttcatt
     8401 ttattttata ttttagattt tagatcttca agaacttctt ccggggtggg gattactcct
     8461 cccatacgtc cgaaatgagt gacttcagtc tgaccgttaa cagcaagtct tacgtcttct
     8521 accatctgtc cggcactcat ctcaactgaa atgatgtgtt tcatctttcc tgcaagttca
     8581 tttattctta ctgaaggaaa aggatatagt gttatcggcc ggatcagtcc ggctttaatg
     8641 ccttctgttc tggcaagttg aacagctttc tggcagactc tggcgcttgt tccgtaagca
     8701 acaaaaagat attcagcatc ttctgtttga aattcctcaa aacggacctc attcttttcc
     8761 atctcacggt atttcgcctg aattttctga ttatgttttt cgtgcaggta tgggtcgaga
     8821 ttgagggagg ttatgatatt tcgttctctt gttggcggtt ttcctgtggt tgcccactca
     8881 ggcaaagttt tttccctctc cctgtaaggt ttgagataaa ctttttccat catctgccct
     8941 atggctccgt cggaaaggat cataaccgga ttgcggtatt taaatgccag gtcgaatgcc
     9001 agctcaacga agtcggccat ttcctgcact gaggcaggtg ccagtaccag aagatgataa
     9061 tcgccatggc caccgccctt gcatgcctgg aaatagtccg actgtgaagg ctgtattgta
     9121 ccaagtccgg ggcctcccct tacaacattt actatcagac atgggagctc ggctcctgca
     9181 atgtatgata tgccttcctg catcaggctt attcccgggg aagaagatgt tgtcattacc
     9241 ttttttccgc aggctgcacc gccatatacc atattgatag ctgccggttc actttctgcc
     9301 tgcaaaacaa ccatccccgt cttttcgtaa ggattagctt ccatgaggta ctcaagtatc
     9361 tcactctgag gagtaatggg ataaccaaaa tatgcatcca cacctgctct tatcgcagcc
     9421 tcggctattg cctcattacc cttcatcagt ttgagttctt tttccatata ttcagatttt
     9481 ctttctgtaa actgttatta ctccgtcggg gcaaaccact gcacaatttg tacatccgtt
     9541 acatgtatcc ggattagccg gataagcata ataatagccc ttgctgttaa cctccgtggc
     9601 aagggctatt gtgcctgtag gacacgtcac cacacatagt tcgcatccct tgcacctttc
     9661 cacttcaatc acaatgctac ctttaatctt tgccattatt actgtaattt aattatttac
     9721 agggtgatcc atatgaaatt tttcaagcct attcttaagt tcgtcaaact ggtttttatg
     9781 aaccatagaa acacaaattc tcaatccttc agtgcgctca ctgtcggtaa ttctcagaga
     9841 tatggcactc attccgtaac gcaagagttc ctcaaggaga ctgctgccag acatacctgg
     9901 atatgatact gtaaaataaa agccatcggc tatgggctcg tcaatatctt tatcgtaaac
     9961 aattttaaac ccgttcgaaa gaaaaagctg cttcattatc tttgcttttt cgccataaat
    10021 ctttacatct tcaacatatc tgtattttcc gtcgttgaca gctttcagca gggctgccag
    10081 accatattgt acagaatgtg tcacgccagc agttaatccg tatagtgctc cgtaaatgag
    10141 tgcctgtcca aagttatcag atttaaaata tctgagcaaa tcggggtatc tcctgttgaa
    10201 aagatggtcg gaaactgcca taagtcctat cctctggcca gcataactga acattttaga
    10261 acttgagatg aggattatat aatccttt
//
LOCUS       DAOSNB010000053        10216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6553_length_10216_cov_4.570515, whole genome shotgun sequence.
ACCESSION   DAOSNB010000053 DAOSNB010000000
VERSION     DAOSNB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6553_length_10216_cov_4.570515"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLG42_03695"
     CDS             <1..253
                     /locus_tag="PLG42_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30348.1"
                     /translation="RKIMDLLAFIFFILQGKPVHAIQIVKAHIHYYNNVRSLKKKREI
                     YPGNHENYSKTKTILNRSLVFEFYIKRNKTYASLIKYFN"
     gene            253..1407
                     /locus_tag="PLG42_03700"
     CDS             253..1407
                     /locus_tag="PLG42_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU30349.1"
                     /translation="MKHRSMALFFIIVFSVYFLVNLYLFFKGYRAVSYFHGNHAIYIT
                     TFIILAFSFIIGKVTERTHPSVFSDILNILGSFWLAFMLYGFLLFLVSDIVYLILRFS
                     GILPVEKIPAFRKWAYISTIVVSLSLIVVGFINALIPVTRHYEINISKAACGIKNLRI
                     AAVSDIHLGSIIRKRSIVKLSRMLSDVKPDVIFLLGDIVDGEIGPVMRNDLLKYFNCP
                     KCRDGLLAITGNHEFIGGASVTIPYIEKNGIRILKDEAVVIDGCIQVIGRLDRDGLRY
                     RNEGRKPLSKLMEQVDSSKPVILLDHQPFNLDEAEKAGVDLQLSGHTHNGQMWPLNYI
                     TKKIYEVSSGYKKKGNTHIIVSSGFGIWGPRVRLGSRPEIIVVDITFSGN"
     gene            complement(1416..2708)
                     /gene="murF"
                     /locus_tag="PLG42_03705"
     CDS             complement(1416..2708)
                     /gene="murF"
                     /locus_tag="PLG42_03705"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOU30350.1"
                     /translation="MKTEQLYSIFRESKGVSTDSRTVNKGEMFFALWGNNYNGNQYAA
                     EALGKGACCAVIDDPLYETEGTILVDDCLLELQALATHYRKELGIPVLAITGTNGKTT
                     TKELINAIMAKSKKVHASKGNLNNHIGVPLTILSAPPDTELMIIEMGASHQGEIRTLC
                     MIARPDYGIITNIGSAHLEGFGTFESIVKAKTELYEYLKKVNGVAFYNDNNPLITEQI
                     YRTVNRAVPYSAPDGTPMTVEVVSAEFNLVVKVYFRKKEYIINTNLFGRHNEENIRAA
                     LTASIFLGATMEDAVAAIESYKPELNRSQIRVTEKNTVFCDSYNANPESMRSAIISFS
                     EIKADKKLIILGDMLELGGKSQDEHLAILKMLNSMGISNVMLVGPVFKSVAKEFGYKT
                     FYDSERLREYLKIKPVEGAHILVKGSRGMMLEKIYDML"
     gene            complement(2874..4388)
                     /locus_tag="PLG42_03710"
     CDS             complement(2874..4388)
                     /locus_tag="PLG42_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU30351.1"
                     /translation="MAIKHKRVMYRFKAFPVVLISVLLLSSCSLFKGKKSEVSPTTGW
                     RYNDPDMGGFEVAMGVEQKTGPGLVLIEGGVFRMGQVQQDVMFDWNNPPRTVTVSSFY
                     MDETEIRNVDYREYLHWLRRVYKSYPEVYRRALPDTLVWRSPMGFNDPYVQYYFRHPA
                     YNNYPVVGVSWVQASDYCLWRTDRVNESILIAEGELKPDPNQADENNFNTEAYLAGQY
                     EGVVGKLRPSLSGDKTERRTTFEDGILLPRYRLPTEAEWEFAAYGLKGNTFQERIYER
                     RIYPWDGHNVRNSSRKHMGQMMANFQRGRGDLMGVAGSLNDAGSITVDVKSYWPNDYG
                     LYCMAGNVNEWVLDVYRPLSFYDMDEFNPFRGNVFTDLRRDANGQVVQKDRLGRLPID
                     TVKPQDVADRYNYRKGDYRNYRDGDKKSSLDQNEWNAAEDELESSTRMYAQSPDDFTS
                     LINDNARVYKGGSWKDRAYWLSPSARRFLDQNASRDDLGFRCAMIRVGSPSGFR"
     gene            complement(4445..5413)
                     /locus_tag="PLG42_03715"
     CDS             complement(4445..5413)
                     /locus_tag="PLG42_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOU30352.1"
                     /translation="MKKNRILILLAVCFASFTAKPQDNFHGYGFQSIVTLNPAAAGSE
                     GDGTLRMMYIMHYPERNMDLHFLSVSYDTYIPLFHGGIAGFVTDNYLGGVLNNLSGGF
                     SYSYHLQAGRNLFVNAGLSAAFRHRGINASKVILPDQIDPLRGPVLPSGESINNRGRT
                     IFDVGTGFMIMWGDFTSGVSVNHLAKPDIGWNGASEDQLPWHITLFAMKVFNLSRKNE
                     LSLQPVFLFDFCGDEFNVASGATFGNEKFAVSSLLSYSGNGDINLQPGFSLRFSGSLL
                     FYNYCFNLTSENGMLPFTMYHQAGLSLSLNNVDKRKVIKAIKFPNL"
     gene            5515..8943
                     /gene="porU"
                     /locus_tag="PLG42_03720"
     CDS             5515..8943
                     /gene="porU"
                     /locus_tag="PLG42_03720"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOU30353.1"
                     /translation="MKITRIIIMFLFSALNAYAFPLTQAAGEKFADESVLATGKWFKI
                     AVIDEGVYRIPFSTLQKLGLTNPANPRIYGNNFGQLSYYNDSPKPDDLKEIAILLVTG
                     TDGIFNEGDYLLFYAQSTHRWKYDRSTDSYRFIRHNYSDTAYYFLTSSPSPGKYITTV
                     PSLVSAPDYTSFSHDALFIHEIESENLLKSGREWYQPVSALSGIQIRPGFSGLVTGEE
                     LKYRLRVLGRSSVPVMFRLYEGETIHAAVLVPEVNLFSTTGIFARDVTISGTLTPSSQ
                     NPSFEVKFYNNGEQSAKGWLDYLELQGRANTAFNGKTLIFSDSRSVAPGRITEFNVKS
                     SSASISIWDVTNPFEPVNIQFEKTGDYCIFRISTDSLRKFIVFSYDGTITPVINSVPV
                     PNQNLHASPEADMVIVTHPLFYQHAVKLAGIHNSNSGLTSIIVTPEEIYNEFSGGIPD
                     IVAIRNFLRMKFLKQSGTSHPLKYLLLFGDGSYDNKKLPPRNPNFIPTYQSQNSTIVT
                     SSFVSDDFYGLLEDGEGEATGTEDIGIGRFPVIDTMQASVILSKIIRYMDPSNGGDWR
                     NMVVLTADDEDSNTHMNDSEGLSSLIETKYPEINIEKIYLDAFRQVTSINGQSYPDVE
                     KAINDRINSGCIIFNYLGHGNEVGLAHERVVRTQSVNSWKNDGKLPLFITATCEFSRF
                     DDAELNPFTGEMTAKSSAGEMVLLNPDGGGIALMSTTRVVYSAPNYTLNRNIMYYAFE
                     RDSTGELLRLGDVIRLAKNNSGSGMNKRNFTLLGDPALRLAYPWHGRVVTDSINGVSI
                     TSFTDTLKAVSVVTVSGHIEDNKGNYLNDFNGLIFPVVFDKSRKIKTLANDGGQPMEF
                     YVQNSIVFSGKTKVNNGRFRFSFIVPRDIDYSFGNGKISYYAKDDKTDMNGAFSNFII
                     GGFSSLARIDTTGPEIRLFLNDTLFRNGDISDSNPVLLAKISDLSGINTTGAGIGHDI
                     TAWLDEERNSSFSLNNYFETDMDNYTRGTVKYRLNNISEGVHTLSLKAWDNFNNSAVA
                     TLKFVVRSEGKFIINDVSNYPNPVTSYTYFTAGHNRPDKDLYIIIEIFDMSGRKVKIL
                     EENLTSTGYRLPPIYWDGTIEGGKKAGRGIYAYVVTIKTCDGETARASGRLIIL"
     gene            9013..10179
                     /gene="porV"
                     /locus_tag="PLG42_03725"
     CDS             9013..10179
                     /gene="porV"
                     /locus_tag="PLG42_03725"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOU30354.1"
                     /translation="MKKTLVALLLGSLLILTVKKLDAQSSSDELNAIQTVVPFLTIAP
                     DSRAGAIGDAGVATSPDIYSMHWNPSKFAFIDGKGGFGISYSPWLRTLVPDINLAYLS
                     GYKRIDSRQVVASSLFYSSLGDIPFTDDFGNLQRVFIPTEFAIDAAYARLFGENFSGG
                     IAFRFIYSNLTGGFTSGGTETHPGVSFAADVSGLYRKETEILNKNALLSIGFNFSNIG
                     SKMSYSKSKEPDFIPMNMRVGVGCTFKFDNFNELTLTTDFNKLLVPTPPKYDPDRNII
                     AGKDPNVSVPVAIIQSFFDAPGGLKEELREITVSMGAEYWYNQQFAIRGGYFYENETK
                     GNRKYFTAGAGFRMNILEIDFSYLMPVVQNHPLARTLRFSLSFEFSSLRNASKG"
BASE COUNT         2980 a         2172 c         2062 g         3002 t
ORIGIN      
        1 aagaaaaatc atggacctgc ttgcttttat ctttttcatt ctgcagggta aaccagtgca
       61 tgcaatacaa atcgtcaaag cacatataca ctattataat aatgtgcggt cgcttaaaaa
      121 gaaacgggaa atttacccgg ggaaccatga aaactacagc aaaacaaaaa ctatactgaa
      181 ccgaagcctt gtttttgagt tttatattaa aagaaacaaa acctatgcaa gtcttataaa
      241 atattttaat taatgaaaca cagaagcatg gcactatttt ttataattgt tttttccgtt
      301 tattttcttg tgaacctgta tctgtttttt aagggttaca gggcagtttc atattttcat
      361 ggcaaccatg caatttatat aaccaccttt ataatactgg ctttttcctt tatcatcggc
      421 aaagtaactg aacgaactca tccttcagtt ttttcagata ttctaaatat tcttggcagc
      481 ttctggctgg cgttcatgct ttatggattt ctactgtttt tagtttccga tatagtatat
      541 ctcatactta gattttcagg catcttacca gtagaaaaaa ttccggcatt cagaaaatgg
      601 gcatacattt caacaatagt tgtttcctta tctcttattg ttgtgggatt tattaatgct
      661 cttattcccg ttaccagaca ttatgaaatc aacatcagca aggcagcatg cggcataaag
      721 aatttaagaa ttgcggcagt ttctgacata catctgggga gcataatacg caagcgtagc
      781 attgtaaagc tttcacggat gttgagtgac gtgaagcctg atgtgatttt tttgctggga
      841 gatatagttg atggagaaat cgggccggta atgagaaatg atcttctgaa atattttaac
      901 tgtccgaagt gcagggacgg gctgctggca attacgggca atcatgaatt tatagggggt
      961 gcatctgtta caataccata cattgaaaaa aacgggatcc gtattctgaa ggacgaagca
     1021 gttgttattg acggttgcat acaggttata ggtcggctcg acagggatgg gctcaggtac
     1081 aggaatgagg gaaggaagcc actctcaaaa ctgatggagc aggttgacag ttcaaagcct
     1141 gtaattcttc tcgaccatca accgtttaat cttgatgaag cagaaaaggc aggtgttgac
     1201 cttcagcttt cgggtcatac acataacggg cagatgtggc ccttgaatta tattacaaaa
     1261 aagatatatg aggttagttc aggatacaag aaaaaaggta atactcacat aatagtttca
     1321 tctggtttcg gtatctgggg accgagggtc aggcttgggt ccagacctga aataattgtt
     1381 gtggatatca cattttcagg caattagcct gagagttaaa gcatgtcgta aatcttttcg
     1441 agcatcattc ctcgtgatcc tttcacaagt atatgtgccc cttccacagg ttttatttta
     1501 agatactccc tgaggcgttc tgagtcgtag aaggttttgt atccgaattc ttttgcaaca
     1561 cttttgaaca ctggcccaac aagcattaca ttgcttattc ccattgaatt cagcatttta
     1621 agaatagcca gatgctcatc ctgtgattta ccgccaagtt cgagcatgtc cccgagaata
     1681 atcagctttt tatcagcttt gatttcactg aatgatataa ttgcgcttct catgctttcg
     1741 gggttagcat tgtaagaatc acagaaaact gtatttttct ctgtaaccct gatctgcgac
     1801 ctgttcaatt ccggcttata ggattcgatt gccgcaacgg catcttccat cgttgctccg
     1861 agaaaaattg atgcagtaag tgcggctctt atattttcct cattatggcg accaaaaagg
     1921 ttcgtattaa ttatgtattc ctttttcctg aagtaaactt tgacaaccag gttgaattca
     1981 gccgacacga cttccactgt cattggagtt ccgtcgggtg ctgaatatgg tactgctctg
     2041 ttcacagtac ggtaaatctg ttctgtaatt agcgggttat tatcgttgta aaaagctact
     2101 ccgttaactt ttttcaggta ttcataaagt tctgtttttg cttttactat gctttcaaaa
     2161 gtgccaaagc cttcaaggtg ggctgaaccg atattggtta tgatgccata atcggggcgg
     2221 gctatcatgc aaagggttct gatttctccc tgatggctgg cgcccatctc aataatcatc
     2281 agttcagtat cgggaggagc agaaagaatt gtcaggggaa cacctatatg attatttaga
     2341 tttccttttg aagcgtgtac ctttttgctt tttgccatta tggcattaat aagttctttg
     2401 gtagttgttt ttccattagt acctgttata gcaaggaccg gtatgcccag ttctttgcgg
     2461 taatgggttg caagtgcctg caattcaagg aggcagtcat caaccagtat cgtaccttct
     2521 gtctcataca gaggatcgtc tattacagcg cagcatgctc cttttccgag agcttcagct
     2581 gcatactgat ttccgttata attgttcccc cagagggcaa agaacatttc tcctttatta
     2641 acagtacgcg agtcagttga gactcccttc gattccctga aaattgaata aagctgttca
     2701 gttttcattt atacctggtc agattagggt tattctttat aaaacattca gtatgagtat
     2761 taaaagaaca attgcaataa attgattttc aacatcttta aagtcaattg ccttctgtca
     2821 ggtcaaaaaa acaggggctt tatttaagcc cctttaatat tagtttcagt atattatcta
     2881 aatcctgacg ggcttcctac gcgtatcatc gcgcatctga aaccaaggtc atcccttgaa
     2941 gcattctggt cgaggaacct tcttgccgaa ggacttaacc agtaggctct gtctttccat
     3001 gaaccaccct tataaacacg tgcattgtcg ttgataagtg atgtaaaatc atcggggctc
     3061 tgggcataca ttcttgttga tgattctaac tcatcttctg cagcattcca ttcgttctgg
     3121 tcaagggatg atttcttgtc cccatctcta taatttctat agtcgccctt gcggtaatta
     3181 tatctatcag caacatcttg tggttttaca gtgtcaatag gaagccgtcc gagtctgtct
     3241 ttctgcacaa cctgtccgtt ggcatctctt ctcaggtcgg tgaaaacatt ccctctgaat
     3301 gggttgaatt catccatatc atagaatgaa agtggtctgt acacgtcgag tacccattca
     3361 ttgacgtttc ctgccatgca atagaggcca tagtcgtttg gccagtacga tttcacatca
     3421 actgtaatgc ttccggcgtc gttcagactt cctgcaaccc ccatgaggtc tcccctgccg
     3481 cgctggaagt ttgccatcat ctggcccata tgctttcttg atgaatttct cacattatgg
     3541 ccatcccatg ggtaaattct tctttcatat attctttcct ggaatgtatt acctttcagt
     3601 ccatatgctg caaattccca ttctgcttcg gtaggcagtc tgtatcttgg taacagtata
     3661 ccgtcttcaa aggtggtacg gcgttcggtt ttgtcgccgc taaggcttgg cctcagttta
     3721 ccgacaacac cttcatactg gcctgccagg tatgcttcgg tattgaaatt gttttcatct
     3781 gcctggttgg gatcaggctt gagctcacct tcagcaatca atatactttc attcaccctg
     3841 tcggtccgcc acaaacagta atccgatgcc tgcacccagc taacacccac aacaggatag
     3901 ttgttatatg caggatgccg gaagtaatac tgcacgtatg gatcattgaa ccccatcggg
     3961 cttctccata caagtgtgtc gggcagtgca cgcctgtaga cttcagggta actcttgtaa
     4021 actcttctca accagtgaag gtattccctg taatctacat tcctgatttc ggtttcatcc
     4081 atataaaaag atgaaacagt aactgttctt ggcggattat tccagtcaaa catcacgtcc
     4141 tgctgaacct gccccatcct gaatacgcca ccctcgatga gaacaagccc cgggcctgtt
     4201 ttttgctcaa cacccatagc tacttcaaac cctcccatgt cagggtcgtt atatctccag
     4261 cccgtggtgg gtgaaacttc tgatttctta cccttgaaaa gactgcagga tgacagcagt
     4321 agaactgata ttaaaacaac cggaaaagct ttgaatctgt acataactct tttatgtttt
     4381 atggccataa ctgatgagtt caaatataca aaaatagtaa aatatttagt tttaccaaat
     4441 ataactacaa attagggaat tttattgcct ttatcacttt tcttttatca acattattta
     4501 aacttaacga caatcctgcc tgatgataca ttgtaaaagg caacattccg ttttccgatg
     4561 ttaaattaaa acaataattg taaaaaagca aagagcctga aaatcttaaa gaaaaaccag
     4621 gttgcaggtt gatgtctcca ttcccactgt acgatagtag tgaactaaca gcaaactttt
     4681 cgtttccaaa cgttgcgccg gaggcaacat tgaactcatc tccgcagaag tcaaataaaa
     4741 ataccggctg caagcttagt tcattcttcc gcgacaggtt gaaaaccttc atggcaaaca
     4801 gtgttatatg ccatggtaac tgatcttcag atgccccatt ccatccaata tcaggctttg
     4861 caagatggtt taccgaaact cctgaagtga aatctcccca cattatcata aagcctgtac
     4921 cgacatcaaa tatggttctt cccctgttgt ttatgctttc acccgaaggc agaactggac
     4981 ctctcaaagg atcaatctgg tcgggcaaaa taacctttga agcatttatc cccctgtgcc
     5041 tgaaagcagc actcaggcct gcattaacaa acagattcct ccccgcctgt agatgatacg
     5101 aatatgaaaa ccctccactc aggttattca aaactcctcc cagatagtta tccgttacaa
     5161 aacctgctat tccaccatga aacaaaggaa tatatgtgtc gtatgaaact gataaaaagt
     5221 gcagatccat atttctttct ggataatgca ttatatacat catcctcaga gtaccatctc
     5281 cttcagaacc agctgcagca ggatttagag ttactattga ctggaaaccg taaccatgaa
     5341 agttatcctg cggctttgct gtaaaagaag caaagcaaac agccagcaag atcagtattc
     5401 tgtttttttt cataacacag ttccggcaat atcaacgcgg caatcaacgt taatatttca
     5461 aaaactctaa atataataac aaaataatct cacttcttat ctttgccggg aattatgaaa
     5521 atcacccgaa tcataatcat gtttcttttt tcggcattaa acgcatatgc cttcccgctg
     5581 acccaggcag ccggtgaaaa atttgcggat gagtcagtac tggcaacagg caaatggttt
     5641 aaaatcgctg taattgatga aggcgtatat cgtataccat ttagtactct ccagaagttg
     5701 ggcctgacaa acccggcaaa cccgagaatt tacggaaata atttcgggca gctttcatat
     5761 tacaatgata gtccgaaacc agacgacctg aaagaaatag ccatattgct tgttaccggg
     5821 acagatggaa ttttcaatga gggtgattac cttttattct atgcccagag cactcatcgc
     5881 tggaagtacg accgcagcac agacagttac aggtttatca gacataatta ttccgatacg
     5941 gcatattatt ttcttacatc atcaccttcg ccggggaaat atatcactac tgttccttcc
     6001 ctagtatcgg ctcctgatta tacatccttc tcacatgacg ctttgttcat ccatgaaatt
     6061 gagtcagaaa atcttctcaa atcgggaaga gaatggtacc agcctgtctc tgctctgagt
     6121 ggcattcaga tccgacccgg tttttcaggt cttgttaccg gagaagaatt aaaatacagg
     6181 ctcagagtac ttggaaggtc gtcagttcca gtaatgttca gattgtatga aggtgaaaca
     6241 atccatgcag ctgtacttgt tcctgaagtc aatctctttt ctacaaccgg gatttttgca
     6301 agggatgtaa ccatttcagg aacactcacc ccctcatcac aaaacccgtc gttcgaagta
     6361 aagttttata acaatggaga acaatctgca aagggctggc tcgattacct ggaacttcag
     6421 ggaagagcaa acactgcttt caacggaaaa actttaatct tctccgattc cagatctgtt
     6481 gcccccggca gaataaccga gtttaacgtc aaaagctcat cagcctcaat cagtatatgg
     6541 gacgtcacaa atccttttga gccagtaaac atccagtttg aaaaaactgg cgattactgc
     6601 atattcagaa tatctaccga ttctctcaga aaatttattg tattcagcta tgatggaaca
     6661 ataacccctg taataaacag tgtgccagtt ccgaatcaaa atcttcacgc ttcaccagaa
     6721 gctgatatgg taattgtaac ccatccgctt ttttatcaac atgcagtaaa acttgcaggc
     6781 attcataaca gtaacagcgg actcacatcc attattgtaa cacctgaaga gatttacaat
     6841 gaattttcag gtggaatacc tgatattgtt gcaataagaa attttctcag gatgaaattt
     6901 ctgaaacagt caggcacatc ccatcccctt aagtacctgc tgctttttgg agatggttct
     6961 tacgacaaca agaaattacc ccctcgtaat ccgaatttca tacctactta tcagtcacaa
     7021 aattcaacca ttgttacatc atcttttgtt tctgatgact tctacggact gcttgaagat
     7081 ggtgaaggtg aagccacagg aactgaagat atcggaatag gaaggtttcc tgttatagat
     7141 actatgcagg catctgtaat tctttccaaa ataataaggt atatggatcc ctcaaatggg
     7201 ggcgactggc gtaatatggt cgtattaaca gccgatgatg aggattcaaa tactcacatg
     7261 aacgattcgg aaggactcag ttcattgatt gaaaccaaat atcctgaaat aaatattgaa
     7321 aaaatctatc ttgatgcctt caggcaggtt acatccatta atggacaatc gtacccggat
     7381 gtggaaaagg caataaatga cagaataaac tcaggatgca tcattttcaa ctatctggga
     7441 catgggaatg aggtgggact tgcccacgag agggtggtca gaacccagag tgtcaattca
     7501 tggaaaaatg atggaaaatt gcctctgttt attactgcca catgtgagtt cagcaggttt
     7561 gatgacgcgg aactgaatcc ttttacaggt gagatgacag caaaaagctc agcaggagaa
     7621 atggtactgc tgaatcccga tggaggtgga atcgctctta tgtcaacaac cagggttgtc
     7681 tattctgcac ccaactatac ccttaaccgc aatattatgt attatgcatt tgaaagggat
     7741 tctactggcg agttactgag gctcggggat gtcatacggc ttgcaaaaaa caattcgggt
     7801 agcggcatga ataaaagaaa ctttacgctg cttggcgacc cggctcttcg tctggcatat
     7861 ccctggcacg gcagggttgt taccgattca attaacggcg tcagtattac gtcgtttaca
     7921 gataccctta aagctgtctc agtcgtaaca gtttcagggc acatagaaga taataaggga
     7981 aattacctca acgattttaa cggactcatt ttcccggttg tttttgataa gagccgtaaa
     8041 ataaagacac tggcaaatga cggcggtcag ccgatggaat tctatgttca gaacagcata
     8101 gttttcagcg gaaagacaaa ggttaataat ggcagattca gatttagctt cattgtcccg
     8161 agagacattg attattcctt cggcaacgga aaaataagtt attacgcaaa ggacgacaaa
     8221 acagacatga acggtgcttt cagtaatttt attatcggag gattctccag tcttgcacgt
     8281 atagatacta caggtccgga aataagatta ttcctcaacg ataccctctt cagaaacggt
     8341 gatatttctg acagtaatcc tgttctgctg gcaaagattt cagacctatc aggtatcaac
     8401 accacgggcg ccggaatagg tcatgatata acagcctggc tcgatgaaga gaggaacagc
     8461 tcattttctc tgaataatta cttcgaaacc gatatggaca attacaccag aggcactgtt
     8521 aagtatcggt taaacaacat atctgaagga gttcataccc tctcattaaa agcatgggac
     8581 aacttcaaca attcagctgt tgccacactc aagtttgtag taaggagtga aggaaaattt
     8641 atcataaatg atgtatcaaa ctatccgaac ccggtaacct cgtatactta tttcaccgcc
     8701 ggacataacc gtcccgataa agacctttat ataataattg agatttttga catgagcggc
     8761 aggaaggtaa agatactgga ggagaatctg acctcaacag ggtacaggct gcctcccatt
     8821 tactgggacg gcaccataga gggtggcaaa aaagcaggca gaggcatata tgcttatgtc
     8881 gtcacaataa agacatgtga tggtgaaact gcaagagctt caggcagatt gataatatta
     8941 tagattttat acaataacat gggcagaaaa ttgttttata tttgcggaaa ttcagaatca
     9001 agaaattaca gaatgaaaaa aacgttagtt gcattactgc tgggtagtct tttaatattg
     9061 acggtgaaga aactggatgc acagagctct tctgatgaat tgaatgctat tcagactgtt
     9121 gtcccctttc taacgatagc tcctgattca agggcaggtg caataggaga tgcaggtgtt
     9181 gcgacctcgc cggatattta cagcatgcac tggaacccgt caaaatttgc ttttattgat
     9241 ggcaagggag gtttcggaat ttcatattca ccctggctca gaacactggt acccgatatc
     9301 aatctcgcgt atctgtcggg ttacaaaaga attgacagcc ggcaggttgt agcctcaagc
     9361 cttttttatt cttcacttgg agatatacct tttacagatg acttcggcaa cctgcaaaga
     9421 gtttttatac ctactgaatt tgcaattgat gcagcatatg ctcgtctgtt cggggaaaac
     9481 ttctcaggag gtatagcttt tcgtttcata tattcgaatc ttacgggagg gttcacctca
     9541 ggtggaacag aaacacatcc tggagtctct tttgcagctg atgtttcagg cctttacagg
     9601 aaagaaacag agattctgaa taaaaatgct ctgttgagca tcggattcaa cttttccaac
     9661 attgggtcca aaatgagtta ttcgaaatcg aaggaacccg atttcatacc catgaacatg
     9721 agagtgggtg ttggatgcac ctttaaattt gacaacttca acgaactgac actaaccaca
     9781 gatttcaaca agctgctggt acctacacct ccaaaatatg accctgacag gaacattatc
     9841 gcagggaaag atccaaatgt ttcggtaccg gttgcaatta tacagtcgtt ttttgatgca
     9901 cctggcggtt tgaaggaaga actgagagag ataactgttt cgatgggagc cgaatactgg
     9961 tataaccagc agtttgcaat aaggggggga tatttttatg agaatgaaac aaaaggcaac
    10021 cgaaagtatt tcactgctgg agccggtttc agaatgaaca tcctcgaaat tgatttttcc
    10081 tacctgatgc ctgtggtgca gaaccatccg ctggcacgca cactgcgctt ttcactctcc
    10141 ttcgagttca gctcattaag aaatgcttca aagggatgaa tttccgcaca ggacagggaa
    10201 ttgattttca ccgcct
//
LOCUS       DAOSNB010000054        10113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6653_length_10113_cov_3.965600, whole genome shotgun sequence.
ACCESSION   DAOSNB010000054 DAOSNB010000000
VERSION     DAOSNB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_10113_cov_3.965600"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLG42_03730"
     CDS             <1..474
                     /locus_tag="PLG42_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30355.1"
                     /translation="LTADATDPDGSITRVEYLAGGVKIGESLVPPFSFTFQCDTAGIF
                     EITAVAWDNLNATSTSAPVTISFSLRREYPDLTSLYPSPNNGIFTIELNPRPETVNDN
                     RLTILSLTGKTVFTCQISDEETYKYIDISDAVPGIYIMQITGGNRVLTARRFIKF"
     gene            complement(666..1412)
                     /gene="istB"
                     /locus_tag="PLG42_03735"
     CDS             complement(666..1412)
                     /gene="istB"
                     /locus_tag="PLG42_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOU30356.1"
                     /translation="MSQYIPLIKRQATRLRLSGIGGNITSLLQEAEQDSPSYDEFTHN
                     LFSYEIKGREAKQLTLKMKLARLPLNHDLDSYDFNFASGLSAQQLNQLRELNWLDQCY
                     NIMFSGPSGTGKTYISAGLCYDALRRGYKAYFRGMNDILATLRLKELTPTAVKEFKRL
                     TEAQLIVIDDVMTLPLGREDGNRFFVFLNQIYETTSFIITTNKSPAEWARSVDDETLA
                     TALLDRLLYKCQLIQLQGKSYRMQNRQTIF"
     gene            complement(1417..3003)
                     /gene="istA"
                     /locus_tag="PLG42_03740"
     CDS             complement(1417..3003)
                     /gene="istA"
                     /locus_tag="PLG42_03740"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOU30357.1"
                     /translation="MVKNTEMEARQKQLIIMYHEIRRLRAAEHFSIQRIADQLKINFR
                     TVKKILGMTEEDYDRFTEMKWAKAHHLDPYRDFIVRYLQQYPDTPAAVMHDKLKEQFA
                     TLPKVDPKTVYSYVMMVRRDHNIIRVSASERQYSAVPDLPPGHLAQVDFGEKKLRRSN
                     GEWVKVHFFVMLLCYSRQKFVLFLDRPFTSDDAVIAHEKAFEFFGGIPMEIIYDQDTV
                     FLYRENGGDYIMTDVFHRYEASRGVKVTFCRAGDPESKGKVENSVKYVKHNFLFNRTF
                     INTEILNDQAQAWLERTGNGMVHNTTRKVPQEQWLLERPFLRRWMPLLNAARVNGHKV
                     IKTNTIKYRGNSYSLPFGTYRNDETRVFVTEEGNQLVIKDTGGSIITTHLIPEGVGNN
                     IVNTNHRRDTSIKLDALRGRVREFFGHTIEIDTFIDKINTLYPRYVRDQLTAVLTAAE
                     RSGQQQAEVALEFCVRNSLFSASDFKSILEGQLSGRTHPTALRPVIKPLGDAKTQLIV
                     NIEPERSDIREYEVIFNQSF"
     gene            complement(3282..4658)
                     /locus_tag="PLG42_03745"
     CDS             complement(3282..4658)
                     /locus_tag="PLG42_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOU30358.1"
                     /translation="MKKNRFLQLSDAYSLLIIITLAFGLRLIFFSTVQPWNERITNEP
                     AIESDALEYNQLALSIISDKSFENFNSFRTPGYPLFVALLYSMSSSSVQFVLLVQIFV
                     SLASIFLIYKIAVLFLQPKISLLSAFLFAFDTTQATYTVSLLTETLFVFLFLLSVYCF
                     CNYLKYNNILSLCISALILGLATLVRPILIFFPVVVVPIILIERDMKTTHKLIRILCF
                     VLIFFLTVSPWLLHNYSKYGEAKLSSISGLNLLYYNAAYTEASKTGKSVDEARKEFNI
                     KAAELGADTADIYCFKNSSIYSAIAKQYIKGNLLLYAKQHLKGTINMFKGFGVQKLAM
                     TLNLTAESNHSEYIGRSGIFNRITNFIKGKSAGILLLYLLLGLYLWTNYIFSVYGIFV
                     LCKDRKMIMIILLFILIILYFAALTGVVGYPRYRIPLMPFINILCATGIFALYEKYRS
                     KYVVDLRF"
     gene            complement(4645..6231)
                     /locus_tag="PLG42_03750"
     CDS             complement(4645..6231)
                     /locus_tag="PLG42_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOU30359.1"
                     /translation="MPKTAIIIGAGPAGLTAAYELIQRTDIKPIILEQTSDIGGISKT
                     VNYKGNRMDIGGHRFFSKSDVVMKWWQDIMPIQGSLSKDEILKEIHFKKKFTKLKLSP
                     NGPDPEVTDGVMLIRNRLSRIFYLKKFFDYPVTLNGNTISNLGIVRIIRIGLSYIHIK
                     IKPPKNIITLEDFLISRFGKELYKTFFKDYTEKVWGVPCTEIAADWGAQRIKELSVSR
                     AIIHALKSIFTKETSIEQKNTDTSLIEQFMYPKLGPGQMWETVANIIKEKGGIIINNA
                     KVIGLSIMDNKINEVIYIDNETNNESSFQGDYYFSTMAVKDLIAAMGDTVPPDIKRVS
                     DGLQYRDFITVGLLLNNLKIKNKTSILTLYDLVPDNWIYIQEREVKVGRLQIFNNWSP
                     YMVSDLKKVWIGLEYFCNEGDDLWSMSDKDFIQFAIDELHKIDIIDKNEVIDSTIIRM
                     PKTYPAYFGTYSEFELIKDFTDSFQNLFLIGRNGMHRYNNQDHSMLTAIASVNNIISH
                     ENSKSNIWSINTEGEYHEKK"
     gene            complement(6240..6674)
                     /locus_tag="PLG42_03755"
     CDS             complement(6240..6674)
                     /locus_tag="PLG42_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOU30360.1"
                     /translation="MIDFVKVRKALFTEKTESTVVQLFRYTFVGGLAFIVDFGTLFIL
                     TEYLNLFYLISAGIAFILGLITNYALSVNWVFSNRTLDSKGMEFFLFTVIGLIGLGLN
                     ELLLWTLTDLLSIYYLFSKIITAIIIYLWNFFARKYLLFNKK"
     gene            6886..8184
                     /locus_tag="PLG42_03760"
     CDS             6886..8184
                     /locus_tag="PLG42_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOU30361.1"
                     /translation="MTGYRKLPGLFLFASILTGTLLMSGCVEDPTLPVLTTDEAIEIT
                     TISATVGGNVTEDGGAEVTARGVCWGTAAMPALEDNFKASGTGSGEFSCIIDGLDPNT
                     EYYARAYAENSVGVAYGNEVIFTTGIAPPSVTTGQVTGVTDNSAICSGTVTYNGGAPN
                     TEKGICWSNSPDPDLDDSHVSVTTGSDTYSGTLTGLSSGTRYYARAYVKNEGGTAYGE
                     QVVFVTKVADIEGNLYSTVYIGEQVWMAENLRTTTFTNNAPIPNVTDNTEWSNLNGPA
                     YCWYNNDISYKPTFGALYSWFTIESGALCPSGWHVPSDAEFNALEMFLGMNPDSVNVW
                     GWRGTDQGSQLKSTTSWDDGGNGTNTSGFSGLAGGYRIGLSGNFYAVGILTYWWTATD
                     DSVNDKPEVAWYRRLDGEYNDIYKATTYKRGGKYIRCVKN"
     gene            8281..9579
                     /locus_tag="PLG42_03765"
     CDS             8281..9579
                     /locus_tag="PLG42_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOU30362.1"
                     /translation="MKKGFNYFMLIFLISAIFMASGCKKATVPEVSTVAATEITINSA
                     VSGGTIISDGGEDITEKGVCWSTAVSPTIADSRTNDGTGTDDFVSNIVGLAEGTTYYV
                     RAYATNDVGTAYGNEITFTTGQVGGAVLTTTEVTNVTSSTAVSGGNITDDGGGTITAR
                     GICWGPSANPTIEVNNKTTNGTGPGSFSSNLTGLTDGTIYYYRAYATNSSGTTYGQEY
                     QFITPVTDIEGNLYKTVKIGNQVWMAENLKTTKYNDDTSIPEVTDYTTWGTLTTGAYC
                     WYENDGDYNKSIYGGLYNWFAAETGKLCPTGWHVPTDPEFNTLELTLGLHADSVNIWG
                     WRGTDHGKQMKNTTGWNEGENGTNTSGFSALPGGYRHNTSGSFNGRSTLGYWWTSSDD
                     AANGKPEVGWYRRLDGIYNDVYKASTLKTAGKSIRCIKND"
     gene            9733..>10113
                     /locus_tag="PLG42_03770"
     CDS             9733..>10113
                     /locus_tag="PLG42_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOU30363.1"
                     /translation="MNKYQSDKLRAISFFLILLVIMVHSTNLSVRMDADFTAINKGLN
                     SFLQNFISDGIAKIASPLFFAISGYLFFLNIKEGKPVEFLGKYKKRFRTLVIPYLLWS
                     IFGLIFYFVLQSIPTFKPFFTRELI"
BASE COUNT         2977 a         2083 c         2046 g         3007 t
ORIGIN      
        1 ctgactgctg atgcaacaga ccctgacggt tcgattacca gagttgaata tctggccggt
       61 ggagtaaaga tcggggagag tcttgtaccc cctttttcct ttacctttca gtgcgatacg
      121 gctggcatat tcgagataac tgcagttgcc tgggacaacc tgaatgcgac atccacttca
      181 gcccctgtga caatctcatt cagtttaagg cgcgaatacc ctgacctgac cagtctgtac
      241 ccatcgccaa ataatggtat ctttacaatt gagcttaatc cccgccccga aaccgtgaac
      301 gataacaggc ttactattct cagccttacc ggcaagacag tattcacatg tcagatttcg
      361 gatgaggaga catacaagta catagacatc tcggatgcgg tccccggcat ctatatcatg
      421 caaattaccg gcgggaacag ggtccttaca gccagaaggt tcatcaagtt ttaatccccg
      481 gtctacttct agttcaatac agccgtttgg gcaagttttg ttgtaaacag gaaaaagaaa
      541 tacagtgatt tcgggaaaat gaagtgcagt aaatttggga acaagagtgc agtgatttcg
      601 ggaacagtac tgtagtgact ctggtgtaaa tttatgccta ttttctcgtt agttttcatc
      661 aagttttaaa aaattgtttg tctgttctgc attcggtaac tcttgccctg gagttggatt
      721 agctggcact tgtagagcaa cctgtcaagc agtgctgtag ccagggtctc gtcgtcgact
      781 gatcttgccc attctgctgg ggatttgttt gttgtgatta tgaatgaggt tgtctcatag
      841 atttgattta agaagacaaa gaacctgttg ccatcttccc taccaagagg cagggtcatt
      901 acatcatcaa taacaataag ctgagcttct gtaagtcttt taaactcctt gactgcggta
      961 ggggtaagtt ccttcagccg cagggtggcg aggatatcgt tcatgccccg gaagtatgct
     1021 ttgtatccac gcctgagggc atcataacac agacctgcag agatgtatgt tttaccggtt
     1081 ccggatggtc cgctaaacat gatattgtag cactgatcca gccagttgag ctcgcgtagt
     1141 tggttcaact gctgagccga cagtccggag gcaaagttaa agtcatagct gtcaagatca
     1201 tgatttaacg gcagcctggc aagcttcatc ttcagtgtaa gctgcttggc ttccctgcct
     1261 tttatctcat aagagaacag attatgtgtg aactcatcat aactcggaga gtcttgctct
     1321 gcctcctgga gaaggctggt gatgttaccc ccgatacccg atagccgtag acgtgttgcc
     1381 tgacgcttga taagcggtat gtactgactc atggcatcag aagctttggt taaaaatcac
     1441 ttcgtactcc cgtatgtcac ttcgctcggg ctctatgttc actatcagct gtgtcttggc
     1501 atctcccaga ggcttgatga caggtcgaag cgccgtaggg tgggtcctgc cggagagctg
     1561 gccctcaagg atcgatttga agtcactggc actgaacagg ctgttacgca cacagaactc
     1621 caaggctacc tctgcctgct gctgcccgct tctctctgct gccgtcagaa ctgccgtaag
     1681 ctggtcccgg acatagcggg gataaagcgt attgatctta tcgataaatg tatctatctc
     1741 aatggtgtga ccaaagaact cccttactct gccacggaga gcgtcaagtt taatggaggt
     1801 atccctgcga tggttagtgt ttacaatatt attaccaacc ccctcaggga taagatgtgt
     1861 ggtgattata cttccaccgg tatcttttat taccagctga ttaccctctt ctgtcacaaa
     1921 tactctggtc tcgtcattgc gatatgttcc gaacggaaga gagtaactgt taccccggta
     1981 ctttatggta tttgttttaa taaccttatg accattaact cttgcagcgt taagcagtgg
     2041 catccagcgc cgtagaaagg gcctctcaag cagccactgc tcctgtggca ccttacgagt
     2101 ggtattgtgt accatcccat tgccagtgcg ctcaagccag gcctgggcct gatcattaag
     2161 gatctcagtg tttataaatg tgcggttaaa aagaaagttg tgcttgacat atttaaccga
     2221 gttctcaacc ttgcccttgc tctcgggatc gccagcccgg caaaaagtaa ccttaacccc
     2281 ccggctggcc tcataccgat gaaaaacatc tgtcataata taatctccac cattttcccg
     2341 gtacagaaaa acagtgtcct gatcatagat tatctccatg ggtatacctc cgaagaactc
     2401 aaatgccttc tcatgggcta tgactgcatc atcagaagta aagggcctgt caaggaacaa
     2461 aacaaatttt tgacggctgt aacacagaag catcacgaaa aagtgaacct tgacccactc
     2521 cccgttactt ctccggagct tcttctcccc gaagtcaacc tgggccaggt gcccgggagg
     2581 aaggtccggc actgccgagt actgtcgctc gctggcactg accctgataa tattatggtc
     2641 cctgcgaacc atcatcacat agctgtaaac agtcttcgga tcaaccttcg gcagtgtagc
     2701 aaactgctcc ttgagcttat catgcattac tgctgccgga gtatcaggat actgttgaag
     2761 gtatctgaca ataaaatcgc ggtagggatc aagatgatga gcctttgccc acttcatctc
     2821 tgtgaacctg tcgtaatctt cttccgtcat acccagtatc ttctttactg ttcggaagtt
     2881 gatttttaac tgatccgcta tgcgctgaat ggaaaaatgt tcagcagcgc ggagtcttcg
     2941 gatctcatga tacataatga taagttgttt ttgtcgtgcc tccatctcag tattttttac
     3001 caaaacctga aaggtagcac aggttaataa tcaagttaac ttatcactgt attattgttc
     3061 ccgattttac tgtattcctg ttcccgtttt tactgcattg cagttcccat tttcactgca
     3121 tttttgcttc ccgattacat ttgtgatttt ttaaagcagt tctagcctgt ttttcaataa
     3181 atcagcaaga agctttttga cttcttcatt tttcttgtga cagaaaattg gagttgttga
     3241 atccctgttc agtttttcta gaccacctca tctttattat gctaaaatct taagtcaact
     3301 acgtatttgc ttctgtactt ttcatatagt gcaaatatac ctgttgcgca cagaatattg
     3361 ataaacggca ttaacggaat tctgtatcta gggtatccca caacacctgt gagggcggca
     3421 aaatataaga taataagtat aaaaaggagt attatcatta tcattttcct gtccttacat
     3481 agaacaaaga ttccataaac tgaaaaaatg taattggtcc ataaatataa tcccagcagt
     3541 aaataaagca ataagatacc tgctgattta ccttttataa aattggtaat gcggttaaaa
     3601 attccagatc ttccaatgta ttcggaatga ttagattctg ctgtaagatt taaagtcatg
     3661 gccaattttt gaacaccaaa ccccttgaac atatttatgg ttcctttaag atgctgcttg
     3721 gcatataaaa gaagattacc ctttatatat tgtttggcaa tagctgaata tattgatgaa
     3781 ttcttgaagc aatagatatc agctgtatct gctcctagct cagctgcctt aatattgaat
     3841 tcctttctag cttcatcaac acttttacct gttttggagg cttcggtgta agctgcgtta
     3901 taataaagca aattcaagcc tgagattgaa gacagcttcg cctctccata ctttgaatag
     3961 ttatgtaata accacggaga aactgtcaag aaaaagatta atacaaaaca caatatcctg
     4021 ataagtttat gcgtggtttt catatccctt tcaattagaa ttatcggtac cacaaccacc
     4081 ggaaagaata ttaatatagg acgcaccaat gttgccaagc cgagaataag tgcacttatg
     4141 caaagtgata atatgttatt gtattttaga taattacaaa aacaatagac tgatagtaag
     4201 aacaaaaaaa caaacaatgt ttcagttaat aacgaaacag tatatgttgc ctgagttgta
     4261 tcaaaagcga acaggaaagc agacagtaag gagattttgg gctgaagaaa caaaactgct
     4321 attttataaa tcagaaaaat agacgctaag ctcacaaaaa tttgcacaag caagacaaac
     4381 tggacgcttg atgaggacat gctgtataat aatgcaacaa ataaaggata gccaggtgtt
     4441 ctaaaggaat tgaagttttc aaatgattta tctgatatta tcgataatgc aagctgatta
     4501 tactcaaggg catcactctc gatggctggt tcatttgtga ttctttcatt ccatggttgt
     4561 actgttgaaa aaaaaatcaa tcgtaaacca aaagccagag taattattat gagaagacta
     4621 taagcgtccg atagttggag aaatctattt tttttcatgg tattccccct ctgtattgat
     4681 ggaccaaata ttgctttttg agttttcatg agaaataata ttattcactg aagcaattgc
     4741 agtcagcatt gaatgatcct gattattata tctgtgcatt ccattcctcc cgatcaaaaa
     4801 taggttctga aaactatcag taaaatcttt aattaactcg aactcagaat aagttccgaa
     4861 ataagctgga tatgtcttgg gcatacggat aattgtacta tcaattactt catttttgtc
     4921 aataatatct attttgtgta attcatctat ggcaaactgt ataaaatcct tatcggacat
     4981 tgaccataag tcatcgcctt cattgcaaaa atattctagt cctatccaga cttttttcag
     5041 atcagaaacc atatagggag accagttatt aaatatctgc aaccttccaa ctttcacttc
     5101 ccgttcttga atatatatcc aattatcagg caccaaatcg tataaagtta atattgaagt
     5161 tttattttta atcttcaagt tattcaatag tagaccaacg gttataaaat cacgatattg
     5221 gagcccgtcg cttactctct ttatatctgg cggcacagta tctcccattg cagctataag
     5281 gtcttttacc gccattgtag aaaaataata atctccttgg aatgatgatt cattatttgt
     5341 ttcattgtca atataaatga cctcatttat cttattatcc ataatactaa gaccgattac
     5401 cttcgcatta ttaataatta tccctccttt ctcttttatg atgtttgcaa ctgtttccca
     5461 catttgacct gggcctagtt tggggtacat gaactgttct atcaagctgg tatctgtatt
     5521 tttctgttct attgaagtct cctttgtaaa gattgatttt aaggcatgga tgattgctct
     5581 tgacacagac aattccttta ttctctgtgc cccccaatct gcagctatct cagtgcaggg
     5641 cactccccat accttttcag tatagtcttt gaaaaaagtt ttataaagct ccttcccaaa
     5701 cctactaatt aaaaagtctt ccagtgtaat tatgtttttt ggaggtttta tctttatgtg
     5761 aatatatgac aacccaattc taataattct gacgatccca agattcgata tggtatttcc
     5821 gtttaaagta accggataat caaagaattt ctttaggtaa aaaattcgtg acaacctatt
     5881 tcttataagc attaccccat ccgtaacttc aggatcaggt ccgtttggcg aaagttttaa
     5941 ttttgtgaac ttttttttaa aatggatttc ttttagtatc tcatccttag aaagagatcc
     6001 ttgaattggc atgatatcct gccaccattt catgactaca tcagactttg agaaaaacct
     6061 gtgtccacct atatccatgc ggtttccttt gtaatttacc gttttggata tacctccaat
     6121 atcagatgtt tgttcaagaa ttattggttt tatgtctgtc ctttgaataa gttcataggc
     6181 tgcagttaag ccagctgggc cggccccgat gataatggct gttttaggca tattgtatat
     6241 tatttcttat tgaataacaa atactttctg gcaaaaaaat tccataaata aatgattatc
     6301 gctgtaataa tcttagaaaa aagatagtag atggaaagta aatccgttag agtccatagc
     6361 aataactcgt taagccctag ccctataagg cctattacgg taaacaggaa aaattccatg
     6421 cctttactgt ccagcgttct gttgctgaaa acccagttaa cacttaatgc ataatttgta
     6481 ataaggccaa gaatgaacgc tatgccggct gagataaggt aaaataaatt cagatactct
     6541 gtcagtataa ataatgtacc aaagtcaaca ataaatgcta gccctccaac aaaagtgtat
     6601 ctaaatagct gtactactgt actttcagtt ttctccgtga acaatgcctt gcgaaccttt
     6661 acaaaatcta tcattaataa taagatttag ttagtgatgc taatttgaac gtaattcatt
     6721 gatgtttatt gggggataca agtcattttg gtcaaaatac tctttttggt aagcatgata
     6781 caactaccca ctagtttcta aaactgtctt cgtaaactaa gagtctttca tatacttatt
     6841 aaggcaaaat tacgttcaac tattaattct aattaaaaag atattatgac aggttacagg
     6901 aaactaccgg gattattcct ttttgcatct attttaacgg gtacgctgct catgagcggc
     6961 tgtgtggaag accccactct gcctgtccta accacggatg aggcaatcga aatcacaacc
     7021 atttcagcca ctgttggtgg caatgtcact gaagacggtg gcgcagaggt tacggcacgt
     7081 ggtgtgtgct ggggtaccgc ggccatgcct gcccttgaag ataatttcaa agcatctggc
     7141 accggatcgg gagaattttc ctgcatcatt gacggcctgg atccgaatac agaatattat
     7201 gccagggcat acgccgaaaa cagtgtgggt gttgcttatg gcaatgaggt gattttcacc
     7261 acgggaattg caccgccatc agtgactaca ggacaggtga cgggtgtgac agacaacagt
     7321 gccatatgca gcggcactgt aacctacaac ggaggcgctc ccaacactga gaagggtata
     7381 tgctggagca actcacccga tcctgatctg gatgattctc acgtttctgt aacaacggga
     7441 tcagacacct actccggtac attgaccggc ctttcatccg ggacgaggta ttatgcacgg
     7501 gcttatgtaa agaacgaagg tggaacagcc tacggagagc aggttgtctt tgttacgaag
     7561 gtagctgaca tagagggcaa tctatatagt accgtttaca taggtgaaca ggtttggatg
     7621 gcagagaacc ttagaacaac tacattcact aataacgcac ccatacccaa tgttacagat
     7681 aatactgaat ggtcaaatct taatggacca gcttattgtt ggtacaataa tgatatatca
     7741 tacaaaccaa cctttggggc tttgtacagt tggtttacca tcgaatcagg tgctttatgc
     7801 ccgtcaggat ggcatgtgcc ttcagacgcc gaattcaacg cattagagat gttccttgga
     7861 atgaatcctg attctgtcaa tgtatgggga tggagaggga cggatcaagg ctcgcagtta
     7921 aaaagtacaa caagctggga tgatgggggc aatggaacca atacatcggg attttcaggc
     7981 ctggcaggtg ggtacaggat tggattatcg ggcaatttct atgctgttgg tatattaaca
     8041 tattggtgga cagctactga tgatagtgtt aacgacaaac cagaagttgc ctggtataga
     8101 cgtctcgacg gcgaatacaa tgatatttac aaggctacaa cctataaaag gggggggaaa
     8161 tatatccggt gtgtaaagaa ttagtggtct gattttattg gtttaataag caacaaaaag
     8221 tcaaatttcc agtataaata aacgaaactc aatgagtatg tctaacaata atttgatatc
     8281 atgaaaaaag gatttaatta cttcatgttg atttttctga tttctgcaat ttttatggca
     8341 agtggatgca aaaaagccac tgttcccgaa gtatcaactg ttgcagcaac agaaatcact
     8401 attaattcgg cagtatcagg cggcacaatt atctcagatg gtggtgaaga catcacagaa
     8461 aagggagtat gttggagtac ggctgtgagt ccaaccatag ctgattcaag aacaaatgat
     8521 ggtacgggga ctgatgattt tgtcagtaac atagttggcc ttgcagaagg aactacctat
     8581 tatgtgcgtg cctatgcaac caacgatgtc ggcactgctt acggaaatga aataaccttt
     8641 acaacaggtc aggtaggtgg tgcagtcctt acaacaacag aggttacgaa tgtcacctca
     8701 agcacagcag tttccggtgg taatattaca gatgacggag ggggtactat aaccgccagg
     8761 ggaatatgct ggggtccatc tgcaaacccg actattgaag tgaacaataa gactaccaat
     8821 ggtacaggac caggcagctt tagcagcaac ctgaccggat taactgacgg tacgatatat
     8881 tactatcgtg cctatgccac caacagctca ggtacaacct atggtcagga atatcaattc
     8941 atcacaccgg taaccgacat agaaggaaat ctgtacaaaa ctgttaaaat tggcaaccag
     9001 gtttggatgg cagaaaattt gaaaacaaca aaatacaatg atgatacctc aatacccgaa
     9061 gtgacagact atacaacatg gggtacactg acaaccggtg catattgctg gtacgaaaat
     9121 gatggagact ataataaatc catatacggt ggtttgtata actggtttgc tgcagaaacc
     9181 ggtaaactat gtccgacagg atggcatgtc ccgactgatc ctgaattcaa tacccttgaa
     9241 ctcacactcg gcctacatgc cgattcagta aatatatggg gttggcgagg tacagatcac
     9301 gggaaacaga tgaagaatac aaccggatgg aatgaagggg agaatggtac caatacaagt
     9361 ggtttctctg ccctcccagg tggatacagg cacaatacgt cgggatcctt taatggtcgt
     9421 tccactcttg gttattggtg gacctcaagt gacgatgctg ccaatggtaa acctgaagtc
     9481 ggctggtata gacgtttgga cggcatctat aatgatgtct acaaagcttc tactttaaaa
     9541 acagcaggca agtctattcg ttgtattaaa aacgattgag tgaaccatct ataagaaaaa
     9601 gaggctactt cttcattatg gaggtagcct cttcatttat cttcatatgg taatcctaac
     9661 tcagattgcc tggttctcaa catctagtca attctatcca ttatctctca ctacgtatat
     9721 aaagaaatat aaatgaacaa ataccaaagt gacaagctta gggcaatttc attctttttg
     9781 atcctgcttg taataatggt gcattcaaca aatctttctg tcagaatgga tgcggatttt
     9841 actgcaatta acaagggatt aaattcattc ttgcaaaact tcataagtga tggtatagcc
     9901 aagattgcat ctcctctgtt tttcgccata tcaggctatc tgttttttct taacatcaag
     9961 gaaggaaaac cagtggagtt tctgggaaag tacaaaaaaa gatttcgtac gcttgttata
    10021 ccctacctat tatggtccat ctttggactg atattctact tcgtccttca atcaattcct
    10081 acttttaagc ctttctttac acgagaatta atc
//
LOCUS       DAOSNB010000055        10053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6697_length_10053_cov_4.386777, whole genome shotgun sequence.
ACCESSION   DAOSNB010000055 DAOSNB010000000
VERSION     DAOSNB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6697_length_10053_cov_4.386777"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..604
                     /locus_tag="PLG42_03775"
     CDS             77..604
                     /locus_tag="PLG42_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOU30364.1"
                     /translation="MELALSVILVLMILGALFAIHAKDLLSAVISCSIVGYGLVICFL
                     LLRAPDLAIVQIVVETITLVIMVAVVLDSTRQEPFTKPDPHDYVAIATALLLIVSVFF
                     LLKAATGSLDPFGEGSMRMAKHYIEGAADKTGSANLVTGVIFDFRGYDTLGEATVLVT
                     AVLGALTILRIRGKK"
     gene            686..1126
                     /locus_tag="PLG42_03780"
     CDS             686..1126
                     /locus_tag="PLG42_03780"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOU30365.1"
                     /translation="MKGMTLIVKKTTQLIAGIVFLYGIYIIVHGHLTPGGGFAGGVIV
                     AGSFILLILAYGSDFMHLTREETGSTITENLGILLALMIALAGMITGIKVFFFNWLPH
                     GTVGELVSAGVIPLYNIFVGMEVAASILTIFLALLIFKEEVTKW"
     gene            1120..1458
                     /locus_tag="PLG42_03785"
     CDS             1120..1458
                     /locus_tag="PLG42_03785"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOU30366.1"
                     /translation="MVVYLLCFILFLVGLYGVITRRNLVKIVISLSIMEFSIFLFLAL
                     IGYVHDGVVPIVDPANPDARYVDPLPQAMVLTAIVIGLATTAMLMSVIIRIYRKYKTL
                     DIREIKNLRG"
     gene            1462..3000
                     /locus_tag="PLG42_03790"
     CDS             1462..3000
                     /locus_tag="PLG42_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU30367.1"
                     /translation="MNPLIPLFVAVPLAGAFLTMIFGKFVKNLNRYIGLIVLLFLAII
                     SYYSIVTVSGTVSVYKVGGWEPINGIPIGIYMVMDSLTAILLCIINTIGLLAVFYSFT
                     YISQYTSENYFYALFCLMVAGMNGVVLSGDLFNIYVFLEISAISSYALVAFGVEREQL
                     EATFKYQVLGGLASLLILLGIGFIYWKAKTLNLADIKVALGSAPEKIHYLLVQMLLLS
                     GFGLKAAIIPFHAWLPDAHSSAPSPISAMLSGVLIKAAGIYVILRLFFNMFIISEEVA
                     ILITTLGALSMAVGVFLAIGQWDIKRLLAYHSISQMGYVVMSVGVGMILVARNTKPEV
                     ASLAIAGGLFHLINHAAFKSLLFLNAGAIEYRIGTRNLQEMGGLSQFMPATSSTSFVA
                     SMAISGIPPFNGFFSKLIIIIAAIMAKFYLLALIAVIVSIITLASFMKFQRYAFYNKE
                     VQKDEKSPKEVPFPMVASMVTLGILCLLLSLLVLPQVRDIVLKPAIDILMNSQMYISP
                     ILGM"
     gene            3011..3487
                     /locus_tag="PLG42_03795"
     CDS             3011..3487
                     /locus_tag="PLG42_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOU30368.1"
                     /translation="MAFFILGLVFWLLLTFKLTVANLIAGAVASLLCTLIFARYYFTG
                     VVKFIQPQRWFWFLVYLVRFIFSCIKANLDVAYRVLHPKMPIRPGIVKVKTSLKSDFA
                     KTLLANSITMTPGTLTVDIIDDCFYIHWIYIRSEDPEVYTKMIMGAFEKYIKKFAE"
     gene            3484..3750
                     /locus_tag="PLG42_03800"
     CDS             3484..3750
                     /locus_tag="PLG42_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOU30369.1"
                     /translation="MNTWLEILLYVQIALSGVCLYRIIRGPTIPDRMVAIDIFGILVV
                     GVCAIISILTGKKFIVDIGIAWIILSFIGTLTLAKYLSGKKLNE"
     gene            3753..4070
                     /gene="mnhG"
                     /locus_tag="PLG42_03805"
     CDS             3753..4070
                     /gene="mnhG"
                     /locus_tag="PLG42_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOU30370.1"
                     /translation="MVAMIIISIGVLFNFFGCIGLIRLPDVYNRLQAATKCVTLGCCS
                     ILLGVFIHFGFTDAGIKGLIAIPLLFFSATVAAHALIRGTYHFGVKMSDKSVKDDYKS
                     ASK"
     gene            4067..4672
                     /locus_tag="PLG42_03810"
     CDS             4067..4672
                     /locus_tag="PLG42_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU30371.1"
                     /translation="MKPKIRELKEALVSLFSPAYTTKFPAVEHVPFEKFRGKPVVDNE
                     NCVGCETCANVCPPHAITFTDDTVKGVRIIKRDYGKCIFCGQCEEHCITQKGVKLSDK
                     IFDLAVLDRSKNVEYQEKELLVCKNCGAVITTKEHLMFLHKKLGPKAFASVLNLNVLN
                     EKLKLAEGEDVEVEVKDHLKRKDMFNILCPNCLRLVLVKYL"
     gene            4676..5137
                     /locus_tag="PLG42_03815"
     CDS             4676..5137
                     /locus_tag="PLG42_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOU30372.1"
                     /translation="MIPDLQKLLSQPGKKILFVGIGNLLKSDDGAGVYISRRIRETEN
                     ISALTVEMCIENYIGKINSLQPDILVLIDCADMGEPPGTIKLLKLDEIKDFTTNTHNI
                     SLKKISDFFNAQVFILGIQPLSVAFGEKISYIVARSAKNIIKQINNYKTIK"
     gene            5151..5567
                     /locus_tag="PLG42_03820"
     CDS             5151..5567
                     /locus_tag="PLG42_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30373.1"
                     /translation="MAVEYLCKACRGHLNVKTSIVLAAQKMNSSQRGLVFLNPEIGNY
                     TVTTHPSFQLKEGEEYIFTCPICHSLLNSAKYDHLVRIIMVDEDKKEYDIYFSDVAGE
                     KCTYKIRNSELEEKIGPDADRYSKYFEVPEEDRKYL"
     gene            5721..>6520
                     /locus_tag="PLG42_03825"
     CDS             5721..>6520
                     /locus_tag="PLG42_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOU30374.1"
                     /translation="MTDISKLCADNIRILSMAMVEKAKSGHPGGAMGGADFIHILFSE
                     YLNFDPDDPEWINRDRFFLDPGHMSPMLYSVLTLTGHFTIDDIKNFRQWGSPTPGHPE
                     LDRKRMIENTSGPLGQGHTIAVGAAIAERFLAARFGELFSHKIFTFISDGGIQEEISQ
                     GAGRLAGHLGLHNLIMFYDSNDIQLSTETKAVTSEDTAMKYRAWGWNVLQIDGNDHNQ
                     IRKALDKALNEKERPTIIIGKTIMGKGLLDKDGNSFERKTSTHGMPVS"
     assembly_gap    6521..6620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6621..7304
                     /locus_tag="PLG42_03830"
     CDS             <6621..7304
                     /locus_tag="PLG42_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU30375.1"
                     /translation="MSLPVIYIWTHDAFRVGEDGPTHQPVEQEAQLRLMEHLKNHHGE
                     NSMLVLRPADATETTVAWKLALENRRTPTALLLSRQNIKDLPSPSGNRYNDALGLYKG
                     AYIVEDCEGQPDVILVACGSEVSTLVDGAALLKNEGIKVRIVSAPSEGLFKKQTTEYR
                     KSIIPDNTPVFGLTAGLPVTLQGLAGQRGKIWGLETFGYSAPYKVLDEKFGFTADNVY
                     RQVKELLAM"
     gene            7433..8506
                     /locus_tag="PLG42_03835"
     CDS             7433..8506
                     /locus_tag="PLG42_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30376.1"
                     /translation="MKKIFFAAVIYYLLQVSQALSQTDSVPPLQDINITRTVDTKLFE
                     SDELLDITLRFDITTFKRKRSDTEYFDAVISYLSDKQDTIYQKIKVRARGEFRRTFCD
                     FPPVHLNFRLKDTISGQFSGIDKLKLVPYCKPGYEDYVLREYLVYKLYNVLTDYSLRV
                     RLLRIKYINSAKPEAKPITQFGFVIEPVSLFEKRTGSTEVKTKVSQRMIREEMMNRVA
                     IFNYMIGNTDWSVPAMHNVIVFSQPHTGPKDLGTILPYDFDFSGIVNAEYAHPFESLP
                     IKSIRERLYLGLCRSEESFTKSLAEFVEKKEKFYNVINSFPYLREKSKKDMIMYLESF
                     YHDFDRKNTIVHKLINDCKKFNL"
     gene            8518..9117
                     /locus_tag="PLG42_03840"
     CDS             8518..9117
                     /locus_tag="PLG42_03840"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOU30377.1"
                     /translation="MLEKLLSEVTLANILIRFGFNLLVLTILVYFIYYKHSKKEEYLF
                     SFYLIGVVIFLICALLKTVDIQLGLALGLFAVFAILRFRTVNYTVKDMTYIFAVIGVS
                     VINSQANIPPPVIGAVVINSLIIIIAWLLELYTRKRTLTSFIIQYKNLNLLKPEARQD
                     LLRELSVLTGYDIRNVVIKEMNIEKGSAELEVFFREQPT"
     gene            9197..9802
                     /locus_tag="PLG42_03845"
     CDS             9197..9802
                     /locus_tag="PLG42_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOU30378.1"
                     /translation="MMMNPDIKPGVKGIIFDLDGTLVDSMPYHFAGWKKACEKFGADI
                     DPSFLRLHTGSPGWIIASEIIKTCKLDSSITIDDILKVKLEDFYSNQHLIKPIEPVVA
                     IVKKYYGKLPMAIGTGGHRDAVERTLEVTGLKKYFDIVITANDVQNHKPHPETFLKCA
                     ELMNVEPRFIQVFEDGDLGIEAARRAGMIVTDVREFIPATW"
BASE COUNT         2924 a         1985 c         2162 g         2882 t
ORIGIN      
        1 gttctgtcgg tttattcaat atgggttatt gctggtttgc tgattatgtt actgattatg
       61 atataataag gcaattatgg aactggctct ttcggttatt ctggttttaa tgatacttgg
      121 ggcgctgttt gccatccatg caaaagacct gctctcggcg gtcatttcct gcagcattgt
      181 cggatacgga ctggtgatat gttttctgtt gctgagggca cccgacctgg caatagttca
      241 gatagtggtt gagactatta ctctggtgat aatggtggct gtagtactcg acagcacacg
      301 gcaggagccc ttcactaagc ccgatcctca cgactatgtt gccatagcaa cagcattact
      361 gctgattgtt tctgtcttct tccttctgaa ggcagcaaca ggttcactcg acccattcgg
      421 agaaggctcc atgaggatgg caaaacacta tatcgaaggt gcagcagata agaccggcag
      481 tgcaaacctt gtaactggag taatcttcga cttcagagga tatgataccc tgggcgaggc
      541 aacggttctt gtaacagcag tgcttggagc attgacgata ctgagaatca gaggaaagaa
      601 atgaataaag aacattgaat aaagaaaaga tactttaaaa tttataattc gttaaccggt
      661 ttttgatatt caaaactata catcgatgaa aggaatgact ctgatagtaa agaaaacaac
      721 ccagctgatt gctggaatag tatttcttta cggcatttac ataattgttc acgggcatct
      781 tactccggga ggtggttttg ccgggggggt aatcgtggcc ggctcattca tactacttat
      841 acttgcctat ggaagtgact ttatgcatct tacccgagag gagaccggct cgaccattac
      901 cgaaaacctc ggtatcctgc ttgccctaat gatagcactg gcaggcatga ttaccggaat
      961 taaggttttc ttctttaact ggctgcccca cggaacagtt ggagaacttg ttagcgccgg
     1021 agtaatacca ctgtacaaca tatttgtcgg tatggaggtc gcagcctcaa tactgacaat
     1081 attccttgca ttattaatat ttaaagagga ggtaacaaaa tggtagtcta cctgctttgc
     1141 ttcatattat tccttgtcgg actctacgga gtgataacca gaagaaacct ggtaaaaatt
     1201 gtcatttcgc tttcgataat ggaattcagt atttttctgt ttcttgcact aatcggctac
     1261 gtgcacgacg gcgttgtacc gatagtcgat cctgcaaacc ctgatgccag atatgtggat
     1321 ccactacccc aggcaatggt gcttacagca attgtgatag ggcttgcaac cacggcaatg
     1381 cttatgtcgg taataatcag aatatacaga aaatataaaa ctctggatat cagagaaata
     1441 aaaaatctaa gaggataaaa gatgaatccg ctgataccat tatttgttgc tgttccgctg
     1501 gcaggagctt tcctgacgat gattttcgga aaatttgtaa agaaccttaa caggtacatc
     1561 ggactcattg tcctgttgtt ccttgccata ataagttatt attctatagt tacagtttcg
     1621 ggcactgttt cggtttacaa ggttggtggc tgggaaccta ttaatggcat tccgataggc
     1681 atctacatgg taatggatag tctgacagcc atccttcttt gcatcataaa caccattgga
     1741 ctgctggcag tattctattc atttacttat atcagccagt acacctccga aaattatttc
     1801 tatgccctgt tctgcctgat ggttgcagga atgaatggtg ttgtgctcag cggcgacctg
     1861 ttcaacatat acgtcttcct cgagatttca gcaatatctt cctatgcgct tgtagcgttt
     1921 ggcgttgaaa gggaacagct cgaagccacg ttcaaatatc aggtccttgg cggacttgct
     1981 tcgctgctta ttctgcttgg tataggattc atctactgga aagctaagac actcaatctt
     2041 gccgatatta aagttgccct cgggtctgcc cctgaaaaga tccattatct ccttgtgcag
     2101 atgcttcttc tgagtggctt tggcctgaag gcagctatca ttccttttca tgcatggtta
     2161 cccgatgcac attcatcggc tccctcgcca atttctgcaa tgctttcggg cgtgctgata
     2221 aaagctgccg gtatctatgt gatactcaga cttttcttta atatgttcat tatcagcgaa
     2281 gaagtggcaa tactgattac cacactcggt gccctctcaa tggctgtagg cgtattcctt
     2341 gccataggcc agtgggatat caagaggctt cttgcatacc acagcatcag ccagatggga
     2401 tatgttgtta tgtcggtggg agtaggtatg attcttgttg caaggaacac aaagcctgag
     2461 gttgcatctc ttgccattgc aggcggtctg tttcatctta taaaccatgc cgccttcaag
     2521 agccttctct tcctcaatgc tggagccatc gaatacagga ttggaacacg caacctccag
     2581 gaaatgggtg gattatcaca gtttatgccg gcaacctcat caacctcttt cgtggcatcg
     2641 atggcaatat caggtatccc gccattcaat ggatttttca gtaaactgat catcatcata
     2701 gccgcaataa tggctaaatt ttacctgctc gcacttattg cagttattgt cagtataatc
     2761 actcttgcat cgtttatgaa gttccagagg tatgcattct acaacaaaga ggtacagaaa
     2821 gatgaaaaaa gtccgaaaga agtacccttc cctatggtcg ccagcatggt gacacttggt
     2881 attctgtgcc ttcttctcag cctgcttgta ttgcctcagg tgagggatat tgttcttaaa
     2941 ccggcaatag atattttgat gaattcacaa atgtacattt caccaatatt aggaatgtga
     3001 tatgagatat gtggcatttt ttattctggg tctggtattc tggctgctgt taaccttcaa
     3061 attgacagtg gcaaacctta ttgcaggagc agtggcttct ttgctctgca ctctaatttt
     3121 tgcaagatat tattttacgg gtgtcgtgaa atttattcag ccacagcggt ggttctggtt
     3181 tctggtttac cttgtcaggt ttattttctc ctgcataaaa gccaacctcg atgtggctta
     3241 cagggtactt caccctaaaa tgccaataag gccgggtatt gtgaaagtta aaacaagtct
     3301 gaaatcggac tttgccaaaa cccttcttgc aaattcaatt accatgacac ccggtacatt
     3361 gactgtagat ataattgacg actgtttcta tatacactgg atctacatcc gttctgaaga
     3421 tccggaggtt tatacaaaga tgataatggg tgcttttgaa aagtatatta aaaaatttgc
     3481 cgaatgaaca cctggcttga aatattgttg tatgtgcaga ttgccctgag tggcgtctgc
     3541 ctttaccgga tcatccgggg tccgacaata cccgacagga tggttgcaat tgatatattc
     3601 ggaatacttg tggtaggcgt atgtgccata atctcaatcc ttacaggcaa aaagtttatt
     3661 gtggatatcg gtattgcatg gattatattg agttttatcg gcacgttaac acttgccaaa
     3721 tatcttagcg gtaaaaaatt aaatgaatga ctatggttgc aatgattatc ataagtatag
     3781 gtgtcctttt caattttttc ggttgtatcg ggctaataag actacctgat gtgtacaaca
     3841 ggcttcaggc agccacaaaa tgcgtcactc tcggatgttg cagtatcctt ctgggtgtgt
     3901 ttattcattt cggatttaca gatgcaggaa tcaaggggct tattgcaatt cccctcctgt
     3961 tcttcagtgc tactgttgct gctcatgcac tgatacgcgg cacctatcat ttcggtgtta
     4021 aaatgagcga taagagtgtt aaggatgatt ataaaagtgc ctcaaaatga aaccaaaaat
     4081 acgtgaactg aaagaagcgc tggtttcact gttctcgccg gcatacacaa caaagttccc
     4141 tgctgtggag catgttccct tcgaaaaatt caggggtaaa ccggtagttg acaacgaaaa
     4201 ctgcgttgga tgcgaaactt gcgcaaacgt ttgtccgcca catgccataa ccttcaccga
     4261 cgatactgta aaaggtgtac gtataatcaa aagagactac ggaaaatgta ttttttgcgg
     4321 acaatgtgaa gaacactgca taacgcagaa aggtgtaaag ctttctgata aaatatttga
     4381 tcttgctgta ctcgaccgct caaaaaatgt tgaataccag gaaaaagaac ttcttgtatg
     4441 caaaaactgc ggcgctgtta ttacgacaaa agaacacctg atgtttctgc ataaaaaact
     4501 tgggccaaaa gcctttgctt ctgttttaaa ccttaatgtt ctgaatgaaa aactgaaact
     4561 tgccgaagga gaggatgttg aagttgaggt gaaagatcac cttaaaagga aagatatgtt
     4621 taacatcctc tgtcccaatt gcctgagact cgtgctggta aaataccttt agggagtgat
     4681 tccagatctg caaaaactgc tgtcgcaacc cggcaaaaag attctttttg ttggtatcgg
     4741 caatttgctt aaaagcgacg acggtgcagg agtttatatc agccgcagaa taagagaaac
     4801 tgaaaacatt tcagccctta ccgttgagat gtgcatagaa aactatatcg gaaagataaa
     4861 ttccttacaa cctgacattc tggtactcat tgactgtgcc gatatgggcg aaccaccggg
     4921 aaccattaaa ctgctcaaac ttgacgagat aaaagatttc actacaaata cacataatat
     4981 ctcacttaaa aaaatttccg actttttcaa tgctcaggta tttattctgg gaattcagcc
     5041 cctgagtgtt gcttttggtg aaaaaatttc gtatattgtt gccagatcag caaagaatat
     5101 tatcaaacag ataaataatt ataaaacaat taaataaaga ggaggtcacc atggcagttg
     5161 aatatttatg caaagcatgc aggggacacc ttaatgtaaa aacttcaatc gtcctggcag
     5221 cacaaaaaat gaacagttcc cagagagggc ttgtatttct caacccggaa atcggaaatt
     5281 atactgttac aacacatcct tcgtttcagc tgaaggaagg tgaagagtat atttttacct
     5341 gtccgatttg ccactcgttg ctcaacagcg cgaaatatga ccatctggtc agaataatta
     5401 tggttgatga ggataaaaag gagtatgaca tttacttctc cgatgtagca ggcgaaaaat
     5461 gcacttacaa aataagaaat tcagaacttg aggagaaaat cggacccgat gccgaccgtt
     5521 attcaaaata ttttgaggta cctgaagaag acagaaaata tctgtaattc cgtaagcaat
     5581 taatatcagg tttccgcaac ttatcattta tcgccatgaa cgcaagcaat taaaaattgg
     5641 gttgcgttct tttttgtttt agatatattt cttcattccc aaatagtttt aattttgccc
     5701 gggttaaaaa catattcatc atgactgaca taagcaagct ttgtgccgac aacatcagga
     5761 ttctttcaat ggcaatggtt gaaaaggcaa aatcgggtca cccgggtggt gccatgggcg
     5821 gtgccgattt tatccacatc cttttttcgg agtatcttaa tttcgacccc gacgatcccg
     5881 aatggataaa ccgcgacagg tttttccttg atccaggaca tatgtcgccg atgctctatt
     5941 cagtactcac cctgacagga cattttacaa ttgatgatat caaaaacttc cgccagtggg
     6001 gtagtccaac gccagggcat ccagaactgg accgtaaaag gatgattgaa aacacctccg
     6061 gtccgcttgg acaggggcac accattgccg ttggtgctgc aatagctgaa agatttcttg
     6121 ctgccaggtt cggtgaactc ttctcacata aaattttcac atttatatct gatggaggaa
     6181 tacaggaaga gatatcgcag ggagcaggcc gtctggcagg tcatcttggg ttgcataatc
     6241 taattatgtt ttacgactca aatgatattc agctctcaac cgaaacaaaa gctgttacct
     6301 ccgaagacac agccatgaag taccgtgcgt ggggctggaa cgttctgcaa attgacggca
     6361 acgaccataa ccagatccga aaggctttag ataaagcatt aaacgaaaaa gagaggccta
     6421 ccatcattat cggaaaaaca attatgggga aaggcctgct cgataaagat ggcaacagtt
     6481 ttgaaagaaa gacttccaca catggcatgc ctgtaagtga nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn atgagtctgc cggttatata tatctggacc catgatgcct
     6661 tccgcgttgg ggaagacggg ccaacccacc agcccgttga acaggaagcg cagctgaggt
     6721 tgatggagca cctcaaaaac catcacggcg aaaatagcat gcttgtctta agacctgccg
     6781 atgctaccga gacaaccgta gcgtggaagc ttgccctgga aaaccgccgc acacccaccg
     6841 cactgcttct ttcacggcaa aacatcaaag acctcccctc cccttcaggt aacagatata
     6901 atgacgccct cggcctgtac aaaggagcat atattgtgga agactgtgag ggccagccag
     6961 atgtcatcct ggtagcatgt ggttcggaag tatcaaccct tgttgacgga gctgctctgc
     7021 tgaaaaatga aggtatcaaa gtgaggatag tatctgctcc ttccgaagga ctcttcaaaa
     7081 aacagaccac agagtacaga aaatcaatta ttccggataa caccccggta ttcggtttaa
     7141 cagcgggttt acctgtcact ctgcagggac tggcagggca aagaggaaag atttggggac
     7201 ttgagacatt cggatattca gccccttaca aagtgctcga cgaaaaattc ggatttactg
     7261 cggataacgt ttacagacag gtgaaagagc tacttgccat gtaagaaaaa aggcttttat
     7321 acattttacg caagagtttc ttctaaaggt ttttatttcc tgaaaaactt ggaaggagga
     7381 aaacatattc cttcaaaaaa ttgattatat ttaaactgaa tgtccgatag caatgaaaaa
     7441 gattttcttt gcagcagtaa tatattacct cctccaggtc tctcaggcat taagccagac
     7501 cgattcagtt cctccgctgc aggatatcaa tatcacaaga actgttgata cgaagctctt
     7561 tgaaagtgat gaacttcttg acattaccct gagatttgac ataacaacat tcaaaagaaa
     7621 aagatctgat acagaatatt ttgatgctgt aatatcatat ctttctgata aacaggatac
     7681 catttatcag aaaattaaag tgagggcacg gggtgagttc aggcgcacat tctgtgattt
     7741 tccacctgta catcttaatt tcagactgaa agatacaatc agcggacaat tcagtggcat
     7801 tgacaaactg aaactggttc catactgcaa gccgggttat gaggattatg ttctcaggga
     7861 atatcttgtt tacaagttgt ataatgtact taccgattac agcttaaggg taaggcttct
     7921 gcgaataaaa tatatcaatt ctgcaaagcc tgaggcgaag cccattacgc aatttggttt
     7981 cgtaattgag cccgtatccc tgtttgaaaa acgaacgggt tcaactgaag taaaaacaaa
     8041 agtctcgcag cgaatgataa gggaagagat gatgaacagg gttgcaatat ttaattacat
     8101 gatcggtaac accgactggt cggttccagc gatgcacaat gtgattgtct tcagccagcc
     8161 tcatacgggt ccgaaggatc tcggaacaat acttccttac gactttgatt tttcaggaat
     8221 agtaaatgct gaatatgcac atccttttga aagcttacca ataaaatcta tccgcgaaag
     8281 gctttatctt ggactttgca gaagtgaaga aagctttaca aaatcgctgg ctgagtttgt
     8341 tgaaaaaaag gaaaaattct acaacgtaat taattcattc ccttatctta gggaaaaatc
     8401 aaaaaaggat atgataatgt acctcgaaag tttctatcat gactttgaca ggaaaaatac
     8461 gatagtacat aaactgatta acgattgtaa aaagtttaac ttataatatg atgcaatatg
     8521 ttagaaaaac tgctttcaga agtaaccctg gcaaatatcc tcattcgatt tggatttaat
     8581 ttattggtgc ttaccattct ggtctatttt atttattaca aacattcgaa aaaagaagaa
     8641 tatctttttt cattttacct gataggagtt gttatatttc tgatttgtgc cctgttgaaa
     8701 acagttgata tacagcttgg gttggcactg gggctatttg ctgtttttgc cattctgcgt
     8761 ttcagaaccg taaactatac ggttaaggac atgacttaca tttttgcggt tattggggta
     8821 tctgttatca attcacaggc aaatatacca cctccggtga taggtgctgt tgtaattaac
     8881 tccctgatta tcattattgc atggttgctc gaactttaca cacgaaaaag gacacttaca
     8941 tcattcatca tccagtataa aaatcttaat ttgctcaagc ctgaagcaag gcaggattta
     9001 ctcagggaac tttcggttct aacaggttac gatatccgga atgttgtcat taaggagatg
     9061 aatatcgaga aaggcagtgc tgaacttgag gtatttttcc gggaacagcc aacttaattg
     9121 aaattcctgt ttacaacttt aatactaatg gctcgagaga ttatccgagc cttttattat
     9181 ttttgcgctt taaattatga tgatgaaccc tgacattaag cctggagtaa aaggtataat
     9241 atttgatctg gacggaacac ttgttgattc gatgccgtat cattttgccg gatggaaaaa
     9301 agcgtgcgag aagtttggtg ctgatattga tccttcattt ctgcgattgc ataccgggtc
     9361 gccgggctgg ataattgcat cagaaataat caagacctgc aaactcgaca gcagtataac
     9421 tattgatgat attttaaaag tcaagcttga agatttttac agcaatcagc atcttataaa
     9481 acctattgag ccggttgtgg caattgtaaa gaagtattat gggaaattgc cgatggcaat
     9541 aggtacaggt ggtcaccgcg atgctgttga acggacgctg gaggtaaccg gtctgaagaa
     9601 atattttgat attgttataa ctgccaatga tgtccaaaac cacaaacctc atcctgagac
     9661 tttcctgaag tgtgccgaat taatgaatgt cgagccacgg tttattcagg tatttgaaga
     9721 tggtgacctt ggcattgagg ctgccagaag agcgggcatg attgttaccg atgtcaggga
     9781 gtttattccg gcaacctggt aatactcaaa gcgcgcgaag ctgaggcccg caaggcagga
     9841 ggggctgtag ttgagggatg aggaatgagg gatgaggaat gaggtatgag gaatgagttg
     9901 aaagaaatat taagccttac ggttgatgat atttatttac gaattaaaat atggggctgt
     9961 ctaaaaagcc tcttttttat tttcacccct gaatcccctg ggaggggaca tattgaatta
    10021 caatatttta gcttttatgt ctttattatg ctt
//
LOCUS       DAOSNB010000056         9889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6825_length_9889_cov_4.413565, whole genome shotgun sequence.
ACCESSION   DAOSNB010000056 DAOSNB010000000
VERSION     DAOSNB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_9889_cov_4.413565"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLG42_03850"
     CDS             <1..1199
                     /locus_tag="PLG42_03850"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOU30379.1"
                     /translation="RALRNSYPGKDSQVAAKLREAGAVILGKANLSEWANFRASRSSS
                     GWSGLTGQTKNPYVLDRNPCGSSSGSGVAVAANLCMTAIGTETNGSIVCPSNNNGIVG
                     IKPTVGLISRSGIIPISFTQDTPGPMARTVEDAARMLGPLTGIDPEDPRTFESQGKAL
                     KDYTPFLKKEGLKGKRIGLVTDLMGFFSRVDTLVMREVKRLKEQGAEIIEVKTPSRGY
                     DEASIQVMLYEFRDGLNRYLSKLDSSVPYRSLSDIIEFNRRDSIELRYFDQRIFEQAN
                     EKGGLDSREYRESLERMLRATREEGIDKLMKEYNLDALMAPTGAPAWKTDLILGDNYL
                     GGTSSLAAISGYPSITVPAGFIDELPVGISFFGKAWSEPVLIEIAYAYEQATKHRKAP
                     KYLPAD"
     gene            complement(1296..2966)
                     /locus_tag="PLG42_03855"
     CDS             complement(1296..2966)
                     /locus_tag="PLG42_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOU30380.1"
                     /translation="MGLADYIVLFGYFVFITAFGIWISRKIKSSDGYFRGERKFKWWL
                     MIGQAFGVGTHAEMPVAQTGATYSHGFATIWYQWKNMLITPFYWLIAPFYRRSERTTV
                     GEIVEDRYGRGMGMVYSVFAITFFVFCMGVMLQGAAKVISVASGNLISPNMVVFFMTV
                     AFLLYSFIGGIVAAAYTDFVQSFLIIALSVMLIPLGLKEIGGFSGMRSVLPENFFDLI
                     NADSGIDAFTIAMLALNGVVGITAQPHMVTMCATGNTERAGRIGQTFGSLVKRLVTIG
                     WALTGLVTATLVVKNNTSLPDQEMAFGYACRELLYPGLIGLMLAAIVSANMSSCSNFM
                     TNTGALFTQNFYKKYINPAASDRQLLWMGRFSGLGLSLLAVLFALSVKNVLHAFLFTE
                     TLAAFMGIMFLGGILWKRANRYGAAAAVISSFVVYYLVNAIDTGQVMLVYKWKPGPFG
                     LAMLAGFFFFWLVSILTKPEKRENIEAFFDNMRRKSDASYSDADGKKPLAEKTGDDLL
                     LLDLPGWLKKDRWKNFFRRYREDLVGFFLSWMVVGLLILIAWAIMQIR"
     gene            complement(3012..4175)
                     /gene="nagA"
                     /locus_tag="PLG42_03860"
                     /pseudo
     CDS             complement(3012..4175)
                     /gene="nagA"
                     /locus_tag="PLG42_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
     gene            complement(4215..4949)
                     /locus_tag="PLG42_03865"
     CDS             complement(4215..4949)
                     /locus_tag="PLG42_03865"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOU30381.1"
                     /translation="MIISINKNPSELGIKAGSLAAEKINNAIREKGEARIVLATGASQ
                     IETLEYLISQNIDWSRVEIFHLDEYIGLPITHKASFRKYLTERVISKIKCKAFYAIET
                     EKDIRELTKFLTDRIREKPVDVGFIGIGVNGHIAFNDPPADFETREAYIVVNLDERCK
                     KQQVDEGWFDSLDQVPDKAVSMTVWQIMQCRTIISAVPHKVKAEAVYNTLTCKVTPEV
                     PATILKKHEDFNLFLDYNSVSKLITF"
     gene            complement(4967..6352)
                     /locus_tag="PLG42_03870"
     CDS             complement(4967..6352)
                     /locus_tag="PLG42_03870"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOU30382.1"
                     /translation="MNRRKFVRNTGVIAAGLGTFGITGLTSCTGVKKKKVIEIVSTAS
                     AFEREPLIRPFGFKGGYMTEIWQTAAMLESSSGIKKVGLCSQSVLWSDANVFATHPED
                     EGNALMYDITRFALELIKGRKFTTPVALLESIIPEVYEFGRRVTGREDLRKTFILNSL
                     VGVDNAAWLLYAAENGFTTFDEMVPEEFKPGLPAHHDKLASIPLMAYTIPVEEIKSAV
                     EQGYFFMKIKIGQPGTQEEMLEKDMERLTAIHKAIGDARTPYTKDGKLPYYFDANGRY
                     EKKETLLRLLDHAKKIGAFDQIAIVEEPFPEEAMIDISDIPVRIAADESAHTDTDALE
                     RIKMGYRAIAVKAIAKTLSMTLKIIKVAHEHNVPCFCADLTVNPILVEWNKNVAARLE
                     AFPGLGLGLVETNGHQNYKNWEVMRTYHPYPDAEWANTRNGIFRLDDDYYQKSGGIFM
                     QSPHYTQMFNI"
     gene            complement(6368..7339)
                     /locus_tag="PLG42_03875"
     CDS             complement(6368..7339)
                     /locus_tag="PLG42_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU30383.1"
                     /translation="MKESTGMSRRHFISSLAMAGATVSMNNISGLFATNSPPVSFGPV
                     HVFSKPLQWLDYNSLAEVLAEAGAEGIDFTVRPGGHVLPEKVKTDLPAAVAAARKMGL
                     RVDMIVTAITDANEKYTEDILRIASEQGIKYYRMGYINYDDSLGVWGTLQKYKPVFKR
                     LEELNRKYNIHGAYQNHYGTRIGGPVWDLYELLKDLDPRYIGCQYDVRHAVAEGGSSW
                     VNGLKLIAPWVKCTDIKDFKWSQDAKGRWAPESVPIGTGMVNFDEYFRIVKSYNIGGP
                     VSIHLEYPPFERYNVEMTDQEKRKLFVKEMRKDIEKVKSFLTKYQLV"
     gene            complement(7352..8761)
                     /locus_tag="PLG42_03880"
     CDS             complement(7352..8761)
                     /locus_tag="PLG42_03880"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30384.1"
                     /translation="MKKNSSILSSRREFIAKTTMATAALALGGNALAAANYNRIYGAN
                     EKIRMGFIGLGNRGSQLLRLFMAQPDVEVAAFCDVYEPYMLRDRSRVEPRWIKELGSQ
                     IPALGETFPNEVKKYSDYRKLLEDKSIDAVVIATPDHWHALQTIHAIESGKDVYVEKP
                     LTITIREGRMMVEAQARSKQIVAVGLNRRGNMVYQKLAKEVTAGKIGRISVARAFRIN
                     NMFPDGIGKYKPENPPKDFNWDMWLGPRAYRPYQYNIAPYKFRWWSDYSSQMGNWGVH
                     YLDVIRWMMGETAPVAVTAQGGKYFVNDDRDIPDTMEVTFEFASGAIAIFCIYEASSG
                     SLFPYGEVELRGTKGTLYASESGYRITGARPGQFQTWSKQIEDETFESKDVPLPDGSS
                     RDSTAGLVRNFLDCIKSRQTPLCPLEEGHRSTTFAHLANIALKMKARLEWDPVKERFT
                     NNDTANQYLHYEYRKPWKL"
     gene            complement(8808..9680)
                     /locus_tag="PLG42_03885"
     CDS             complement(8808..9680)
                     /locus_tag="PLG42_03885"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOU30385.1"
                     /translation="MDAIDKVLTLEGAELDRAGAEECLKGLTEVLSENQRFEKVVRIE
                     EKFPAVTFPGAFPPPLDWTDAERICRENDVDLLFTLEAFDTDTRINYQIVKGGNPKSL
                     LGVLTGTEHRADMQTLVKTAWRIYYPAERLVLDEFPLVHSLNFSASGLTPVIAAAALI
                     DRKEAVKRTGLRAGHIYGERIVPYEITVEREYFVKGTEKFRTAMRKARTGNWDEAAEL
                     WKAETTNPGSKIAGRACYNMAIAAEIKGNLELAAEWLRKAYEDYNLRKALKYLRIIEN
                     RMNKTELVQIQEQY"
     gene            9655..9780
                     /locus_tag="PLG42_03890"
     CDS             9655..9780
                     /locus_tag="PLG42_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30386.1"
                     /translation="MRTLSIASSARESLVASLRSTMPALFTPGGRVTGAGSITLR"
BASE COUNT         2552 a         2277 c         2087 g         2973 t
ORIGIN      
        1 cgagggcttt gcgtaattca taccctggaa aggacagtca ggttgctgct aagttaagag
       61 aggccggggc ggtgatttta ggcaaagcca acctcagcga gtgggcaaat ttcagagctt
      121 caaggtcatc gagcggatgg agcggactga cgggccagac taaaaatcca tatgtactcg
      181 accgtaaccc ctgtggttcc agttctggct cgggtgttgc agtggcagca aacctctgta
      241 tgacagccat cggcaccgaa actaatggtt caattgtttg tccgtcaaat aataacggta
      301 ttgttggcat aaaacctact gtcggactca tcagccgctc aggcattata ccaatatcct
      361 tcacgcagga tacccccggc cccatggcaa gaacagttga agatgctgca agaatgctcg
      421 gtccgcttac aggcattgac cctgaagacc cacggacgtt tgaatctcag gggaaagctc
      481 taaaggacta tactcctttc cttaaaaaag agggacttaa aggtaaaaga attggcctgg
      541 taaccgacct gatgggtttc ttcagcagag ttgacactct ggtgatgaga gaggttaaaa
      601 gactgaagga acaaggagct gaaataatag aagtaaagac accatcgagg gggtatgacg
      661 aagcatccat acaggttatg ctttatgaat ttcgcgacgg actcaaccgg tacctttcaa
      721 aactcgattc cagcgtgcct tacagatctc tgagcgatat cattgagttc aacaggcgcg
      781 acagcatcga actgaggtat ttcgaccaga gaatatttga acaggcaaac gagaaaggcg
      841 gactcgattc gcgcgagtac agggaatcgc ttgaaagaat gctcagggca acccgtgaag
      901 aaggaattga taagcttatg aaagagtata acctcgatgc ccttatggca cctacaggcg
      961 ctcctgcatg gaaaacagat cttatactgg gcgataatta tttaggtgga acatcatctc
     1021 tggcagccat ctcgggatat ccttcaatta ccgttccggc aggcttcatt gatgaactgc
     1081 ctgtaggtat ttctttcttc ggaaaggcat ggagcgaacc tgtgcttatc gaaatagcat
     1141 atgcctacga acaggcaaca aaacaccgca aggcaccaaa atacctgcct gctgattaag
     1201 aattaaaggt ttttgcagtc aggtttattt actgcttttt ctgggttcag ccagccatta
     1261 accttttaat tgaagttgtg gctagtggtc ctgttttatc tgatttgcat tatcgcccat
     1321 gcgataagta ttaagagtcc tactaccatc cagctgagaa agaatcctac caggtcttcc
     1381 ctgtatcgtc tgaagaagtt tttccagcga tcttttttca gccatccagg aaggtcaagc
     1441 aatagaaggt catctcctgt tttttcagcg agaggttttt taccatctgc atcactataa
     1501 gaagcatctg attttcttct catgttatcg aagaaggctt cgatattttc cctcttttca
     1561 ggttttgtaa ggatgcttac aagccagaaa aagaaaaacc ctgccagcat tgcaagccca
     1621 aacggacctg gcttccattt gtagactaac atcacctggc ctgtatcaat tgcattaaca
     1681 agataataaa ccacaaagga tgatataaca gctgctgcag ccccgtagcg gtttgccctt
     1741 ttccataata ttcctcccag aaacattata cccataaatg ctgcaagagt ttcagtgaat
     1801 aaaaatgcgt gcagcacatt ttttacgcta agagcgaaga gaacagcaag cagcgataat
     1861 cccaatcctg aaaacctgcc catccacagc aactgcctgt cgctggcagc cggattgata
     1921 tattttttgt agaagttttg agtaaacagg gcacctgtat tcgtcataaa attagaacac
     1981 gacgacatgt ttgcagaaac aatagctgcg agcatcagac ctatcagtcc cggatagaga
     2041 agttcccggc aggcataacc gaaagccatt tcctggtcgg gcaggctggt attgttttta
     2101 actaccaggg ttgctgtaac cagccctgta agtgcccatc caatggtaac gagtctctta
     2161 accagtgagc cgaacgtctg acctatacgg ccagcccttt cagtgtttcc tgtggcacac
     2221 atagtaacca tgtgaggctg agccgtaata cccaccacac cgttaagagc cagcattgca
     2281 atagtaaatg catcgatgcc gctatctgca ttaataagat caaagaaatt ttcaggtaac
     2341 acacttctca ttcccgaaaa accgcctatc tcttttaatc caagaggaat gagcatgaca
     2401 gaaagagcaa taatcagaaa agattggaca aagtcggtat atgcagcagc tacaattcca
     2461 cctatgaagc tgtaaagaag aaatgcaaca gtcataaaaa acacaaccat atttggggag
     2521 atcagattgc ccgaggccac cgatattact tttgctgcac cctgcaacat cacccccata
     2581 cagaaaacga aaaatgtaat cgcaaacacc gaatatacca tacccatgcc tctgccgtag
     2641 cgatcctcaa caatttcacc cactgtagtg cgttcgcttc tgcggtaaaa aggagcaatg
     2701 agccagtaaa aaggagtgat aagcatgttt ttccactggt accagatagt ggcaaagcca
     2761 tgactgtaag tagcacccgt ttgtgcaact ggcatttcgg catgtgtccc aacaccgaag
     2821 gcctgtccaa tcattagcca ccacttaaat ttacgctcgc cacggaaata accatcgctg
     2881 ctctttatct tccttgagat ccagatgcca aaagcagtta tgaatacaaa ataaccgaac
     2941 agtactatgt agtcagccag acccatctga ggatttttat gattatatca atttcatcat
     3001 gtgaaagttt tttatgattc cgggtagact ctttttcctg caacccatgt ttccttaatg
     3061 gtaacatggt ttttttcgtc catggtaaat aatatgatat cagctctttt tcctgtttcg
     3121 agtattcccc ggtcggataa ccggtgcact cttgccacat tctttgttgc caggtttaca
     3181 gcctgcttca gtgaacaacc cgtaaaattt atcattgtct gaatgccttt tttcaaaggc
     3241 atagaggctc ctgccaggac gttcagagca gggttgagta taagaccttc ttcattcatg
     3301 atcactttgc tacccatata ctcgtattca cccggaggca tacctgcaag atgtgtcaca
     3361 tccgatgtaa taataatgtt gttctcacct ttgactttaa agaacaccgt cagctcttca
     3421 ggcctgagat gaaatccgtc ggcgataacc gatggagtaa gtttgtcgca ggcaagttgt
     3481 ggctagagag gattaacatg ccggttgatt gtgtttgcgc atccgtttcc aagatgcgtc
     3541 gatagtgtcg caccataatc tactgctcgg tagattattt cagctgttgc atttgtatga
     3601 ccaatagata ccaatacacc gctttctgtg catttcttta tgaaatcgag accaccttcc
     3661 acctctggtg ccacagttgt aagaattatc tttccgcctg cagcctcctg aaattccata
     3721 aactcatccc atgatggctt gcggatttcc ttaaaaagat gacaaccgta aaatcctttt
     3781 tctgtcgaaa tataaggacc ttcaagatgg aaccccggta ctgaacccct gagatagtcg
     3841 tcattataga cctgtgaaag aattctgaaa ttccggatta gattctcctt gctttcagtt
     3901 accagggtag ggataaacgt tgttactccg tctttccata atgctcttgc agcggttctc
     3961 atccgttcaa cagtaaaaat ctcatcctga aaattgatcc cgacatatcc atttgtctga
     4021 ttatcaatga gccctggaga aataaaaggc acattctcct tcttctcctg tgccgtatga
     4081 attgtgatac tttttatgag actgtcagag atttctactg ttacaaattc acctgtttca
     4141 taatggattc ctgatattgt ggtattcttt atcattttgg taggtattga gcaaaataaa
     4201 aaagtggcta attgtcagaa tgtaattaat ttagaaactg aattataatc gagaaaaaga
     4261 ttaaaatcct cgtgtttttt aagtatggtt gctggcactt caggagtaac cttgcatgta
     4321 agtgtgttgt aaaccgcttc ggccttgact ttatgaggta cagctgaaat tattgtcctg
     4381 cattgcatta tctgccatac ggtcatcgaa acagctttat caggcacctg atccagtgag
     4441 tcgaaccagc cttcatcaac ctgctgtttt ttgcaccttt catcgagatt tacaacaata
     4501 tatgcttctc tggtttcaaa gtcggcagga ggatcgttaa aagcaatatg accgttaacg
     4561 cctattccta taaatcccac atcaacaggc ttttctctta tccggtcggt aagaaatttc
     4621 gttagttccc tgatatcttt ttcggtttca attgcataga aggctttgca ctttatcttg
     4681 cttatgactc tttcggtaag gtattttcta aaacttgctt tatgagttat cggcagacca
     4741 atgtattcat caagatggaa tatttcaacc ctgctccaat cgatattctg tgaaataagg
     4801 tattcgagtg tttcaatctg tgaggcgcct gtggcgagta caattcgagc ttctcctttt
     4861 tccctgatgg cattgtttat tttttctgct gcaagtgagc ctgcttttat accgagttca
     4921 gatggatttt tatttatcga aataatcata acaatttaat gatcagttaa atattaaaca
     4981 tttgggtgta gtggggtgat tgcataaaaa tacctccgct tttctggtaa tagtcatcat
     5041 cgaggcggaa gattccgttt ctggtattag cccattctgc atcggggtag gggtggtaag
     5101 tgcgcatgac ctcccagttt ttgtagttct ggtgtccatt tgtctccacg aggcctaaac
     5161 cgagtcccgg gaatgcttcg agtcgtgcag ctacattttt attccattcc acgaggatag
     5221 ggttaacagt aaggtcggcg cagaagcatg gcacattatg ctcgtgtgca actttgataa
     5281 ttttgagtgt catgctgagt gtttttgcaa tggcttttac tgctattgcc ctgtagccca
     5341 tctttattct ctcgagtgca tcagtgtcgg tatgggcgct ttcgtcagca gctattctaa
     5401 cgggtatatc cgaaatatca atcattgctt cttcagggaa gggctcttct acaattgcta
     5461 tctggtcgaa agctcctatt ttctttgcat ggtcaaggag tctgagcagt gtctccttct
     5521 tttcataacg tccattggca tcaaagtaat aaggaagttt gccatctttt gtatagggtg
     5581 tgcgggcatc acctatggct ttatggatag cagtaagacg ctccatatct ttttcaagca
     5641 tctcttcctg cgttccaggt tggcctatct tgattttcat aaagaaatag ccttgctcaa
     5701 ctgccgattt tatctcttct acaggaatag tgtatgccat cagaggtatg ctggcaagtt
     5761 tatcatggtg agctggaagt cccggtttga attcttcagg taccatctca tcaaatgtgg
     5821 tgaagccatt ttcagctgca taaagcagcc aggcggcatt gtctacacct acaagagagt
     5881 ttaggataaa agtttttctc agatcctcgc gtccggtaac acgacggcca aattcgtaca
     5941 cttcaggaat tattgattca agcagagcga caggggtagt gaatttcctg ccttttatca
     6001 gttcaagagc aaaacgtgtt atgtcgtaca tcaatgcgtt accttcatct tcagggtgtg
     6061 tagcgaatac atttgcatca ctccacaaca cgctctggct gcatagtcca acctttttaa
     6121 tgcctgaact gctttcgagc atggctgctg tctgccatat ctcggtcatg taaccacctt
     6181 tgaatccaaa aggtcttatt aaaggttccc tttcaaaagc cgaagcagtt gagactattt
     6241 cgattacttt tttctttttt accccggtac acgatgttag gcctgtaatg ccaaacgttc
     6301 cgagccctgc tgctataact ccggtatttc tgacaaactt ccgacggttc atttttctgg
     6361 tgtgatttta tactaattga tattttgtaa ggaacgactt cactttttca atatcttttc
     6421 tcatttcttt cacaaagagt tttctttttt cctggtcggt catctccaca ttataccttt
     6481 caaaaggagg atattcgaga tgtattgaaa ccgggcctcc tatgttataa ctcttcacta
     6541 ttctgaaata ttcatcgaag tttaccattc ccgtgcctat tggtacgctc tctggagccc
     6601 atcttccctt cgcatcctgc gaccatttga aatctttaat atcggtacat ttgacccatg
     6661 gggctatcag tttaagtccg ttgacccatg agctgccacc ttctgcaacg gcatgcctca
     6721 cgtcgtactg gcagccgata tatcggggat caaggtcttt aagtagctca taaaggtccc
     6781 acaccgggcc tcctattctt gtaccgtagt ggttctgata ggctccgtgg atattatatt
     6841 ttcggttcag ttcttcgagt cttttaaaaa cgggtttata tttctggagt gtgccccata
     6901 caccaagcga gtcgtcgtag ttgatatatc ccatcctgta atatttaatt ccctgttctg
     6961 acgctatacg caggatgtct tcagtatatt tttcgtttgc atcggtgatg gcggtaacaa
     7021 tcatatctac tctgagtccc attttacgtg cagcggccac tgctgccggg aggtctgttt
     7081 ttaccttctc gggaaggaca tgaccacccg ggcgcaccgt aaagtctatg ccttcagctc
     7141 ctgcctcggc caggacttct gcaagggaat tataatcaag ccattgcaaa ggctttgaaa
     7201 aaacatgtac cggtccgaaa gagactggag ggctattggt tgcaaataaa ccagatatat
     7261 tattcatcga aaccgtggcg cctgccattg caagtgatga gatgaaatga cggcggctca
     7321 tgccggttga ttctttcata ggctcattat tttagagttt ccagggtttt cggtattcgt
     7381 aatgcagata ctggtttgcg gtgtcgttgt ttgtaaatct ttcttttacg ggatcccatt
     7441 caagcctggc tttcatctta agtgctatgt ttgcaagatg tgcaaatgtt gtggaacggt
     7501 gtccttcctc aagcgggcag agcggtgtct ggcgcgactt tatgcaatca aggaagttgc
     7561 gtaccagtcc ggcagtactg tcgcggctgc ttccatcggg aagaggaaca tcctttgatt
     7621 caaaggtttc atcttcaatc tgtttgctcc atgtctggaa ctgtcctggc cttgccccgg
     7681 tgattctgta accgctttcg ctggcataaa gagttccttt ggtacccctc agctcaactt
     7741 cgccataggg gaaaagactt ccgctgcttg cctcatatat gcagaatatg gcaatagcgc
     7801 ctgaagcaaa ttcgaaggtc acctccattg tgtcgggaat gtcgcggtcg tcattaacaa
     7861 aatattttcc tccctgagcc gtaacagcta cgggagcagt ttctcccatc atccatctta
     7921 ttacatcgag atagtgaact ccccagttac ccatctggct tgagtaatcg ctccaccacc
     7981 tgaatttgta tggagcaata ttatattgat acggacgata tgccctcggc cctaaccaca
     8041 tatcccagtt aaaatccttt ggtgggtttt caggtttata cttccctatg ccgtcaggaa
     8101 acatattatt gattctgaat gctctggcaa cagatatcct gccaatttta cctgctgtga
     8161 cctcttttgc cagtttctgg taaaccatgt tacctcttct gttcagccct acagcaacta
     8221 tctgtttgct gcgagcctga gcctcaacca tcatccttcc ttcccttatg gttattgtaa
     8281 gaggcttttc aacatataca tcttttcctg attcaatagc atgaatggtc tgcagagcat
     8341 gccagtggtc gggtgtggca attacaactg cgtcaatact tttatcctcg agaagttttc
     8401 tgtaatcgct gtattttttt acttcgtttg gaaatgtttc accgagggcc ggtatctgac
     8461 tgccgagttc ttttatccat cttggttcca cgcggcttct gtcgcgaagc atgtaaggtt
     8521 catacacatc gcagaaggct gcaacttcaa cgtcgggttg tgccataaaa agcctgagga
     8581 gttgagaccc cctgttgcca aggcctataa aacccattcg tattttttcg tttgcaccgt
     8641 agatgcggtt ataatttgct gcggcaagcg cattgcctcc aagcgccagt gcagctgttg
     8701 ccatggtagt ttttgctata aactccctgc ggctgctgag gatggaacta ttctttttca
     8761 tatatctttt tttgaggttt ttccgggtga atgtataaaa tttaagttta atattgttct
     8821 tgaatttgca ccagttcagt cttattcatt ctgttctcaa tgattctcag atatttcaga
     8881 gctttcctga gattataatc ttcataggct tttcgtaacc attcggctgc cagttcaaga
     8941 ttgcctttta tttctgctgc aatagccatg ttataacatg cacgtcctgc aatcttcgac
     9001 cctggattgg tagtttcagc tttccagagt tctgctgctt cgtcccagtt gccggtacgt
     9061 gcctttctca ttgctgtcct gaatttctca gttcccttaa caaaatattc cctttcaaca
     9121 gttatttcgt atggcactat tcgctcacca tatatatgcc ctgcccttaa tcctgttctt
     9181 ttcacagcct ctttcctgtc gatgagggca gctgctgcaa taacgggagt cagaccactt
     9241 gctgaaaaat tgaggctgtg caccagggga aactcatcaa gaacaagacg ttctgcagga
     9301 taatatatcc tccaggcagt ctttaccagt gtttgcatat ctgcacggtg ctctgtaccg
     9361 gttaaaacac caagcaatga tttcggattt ccgcctttaa ctatctgata gttgattcta
     9421 gtgtcggtat caaaagcttc gagcgtaaac agcaggtcaa catcgttttc cctgcagata
     9481 cgttcagcat ccgtccaatc gagaggagga gggaaagctc cgggaaaagt tacagccggg
     9541 aacttctctt ctattctcac gactttttca aaacgctggt tttctgaaag aacctcggtc
     9601 aggcctttca gacattcttc tgcaccagcc ctgtcgagct ctgcgccttc aagagtgaga
     9661 accttgtcaa ttgcatccag tgcgcgggaa tctttagtgg cttcactgcg gtcgacaatg
     9721 cctgcactct ttaccccggg cggaagagtt accggtgcag gctcaataac attgaggtaa
     9781 agctcctgag tcttgcagga taagaccagt aaagccagca gagggaagat gaaaattagt
     9841 tgtgcaactt tttttctgta aaatcattta agccacgatt aattttttg
//
LOCUS       DAOSNB010000057         9838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6875_length_9838_cov_4.722682, whole genome shotgun sequence.
ACCESSION   DAOSNB010000057 DAOSNB010000000
VERSION     DAOSNB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9838
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6875_length_9838_cov_4.722682"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1255)
                     /locus_tag="PLG42_03895"
     CDS             complement(323..1255)
                     /locus_tag="PLG42_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU30387.1"
                     /translation="MKNRHSRRDFIRLTAAGAAGAFLLTQNACKGTGAKKAAPDPKSF
                     GIGLQLYTIRDEIFKDVPGSLKRVSDMGYKYVELAGYENGKFYGYEPEEFRKLVEDLG
                     MEILSSHTQVEAQGITLENATKMAEDHAKLGVKYCIQPWIVPEARTTIASYQKMAADW
                     NQVGKIMKDHGIMFGYHNHNFEFDTVEGKIPYFDVMMAELDPNYVTLELDVFWVTKAG
                     QNPVDIINKYPGRFELFHLKDMYTKQEPFFTTEGVDDFAPVGEGLIDFKAIIAAKQTA
                     GMKYMIVEQDRTKDGDCFGAIQKSITNLTTKILV"
     gene            1642..4005
                     /locus_tag="PLG42_03900"
     CDS             1642..4005
                     /locus_tag="PLG42_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU30388.1"
                     /translation="MKNSVLIFFLSLLLIPVRSQEPERVFPFRLGAEDLYLPVDTVEM
                     SVISASRSSKKIGELPITIHVITREEIIRNHYYTLIDAIKTLPGVRVSQPGSGELGES
                     FQLRGLTGNLYTMILINGLPVKPSAVVGMPVLAQLPVRQAERIEIIYGPAAAIYGADA
                     VSGVINIITREAEKGTFVLGDVSIGQDNFRNTDFMVGGKAGKDKNILQYSFYGGLNET
                     GDLNIKKKYEQIYNPMHYLHEKGYRYLLGSQYFDPADITEQVLFNYGIRPTDFMRENY
                     MINYDGSINNPEMQDLPGMSSLLGFNLRFRGVSISYNNMYRRLHSSIGQSTYLFKYNN
                     PQNFWGENIRTTTLSYSRDWTSRFSTTTNFSNVTYRMDNNSSMGVTFIDYTDKVYRYA
                     AGRDMLFEQLFTFMPVNSLEIMSGITYQLSGNLPQTNFLDSPFRPGEYKYFSKYVYVE
                     DSVSGRFGINPIVYHNISAFTQTYYSISSFRFMGGLRFDRNSLYGSSINPRLAALYIF
                     NQRTSIRASAGFAYKAPPSSMSYQSLAYKAGTNHDSLIYLTIPNPNLEPEKYMSVELG
                     VARTTRKRVTYDISIYYNSIRNLILNKYVRLSELSLPRAIIKPDTATVLVKENEKSAV
                     SRLYGLQINIKMNDIVKSINLDAELSLSFGKSSQTFPDIIELATNFLGNFKLVPNHFG
                     QLNVSMEPAKNLYIRVSSIWESSWLRVIIPFKDIYNNIFKDFDGFYSMDAVADYRIGS
                     NLSTFIKINNIFDEKYGGPAYSGMNTPLPYSPQLGRSIQIGLTYTLN"
     gene            4005..6572
                     /locus_tag="PLG42_03905"
     CDS             4005..6572
                     /locus_tag="PLG42_03905"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU30389.1"
                     /translation="MRLRIKYPIYFYLLIAWLPAFSESLHGQDIKTTDTLLLKQRMND
                     SLTTVNLLQTAITMIKAGEDESALSVISKAEEIASSDEFLLIEAECQKLAGDIYSRRS
                     DSWEEMLTSYLKAISIYNRTGNREKEAEITKIIADNYFRNGLYKKAALWYQQTFELYK
                     ELKSLNLAGLAELTATAWYNLPDDTLSLKWYNTALSYFETEGNVEGSIRCTRKIASLY
                     AETGNYKEAEEKYFGLLDIYKSRNDSRNISLIYNNLGVIKYRQKELTAALNFFREAEK
                     EQIKSEPDYYFLTDVSSNIAITLQAAGNQAEMMRNFSRALDYARKSERKDEQARIAYL
                     LSLIYFNRGDNYHADLYCKECVESAGASGAYAIMQECYQTWSGVMEKSNDFIKALEYY
                     EKYLNLRDSLNFEKRLAEQKEADRQNYFDQVEQRIKLDIAGEEISNLEMKTLRAESAR
                     RENELKLLLKQQELDRSERERLAQSLALERERFQLNRREQEVMALRQQQRIDSLELVQ
                     KRNEALVLEQNNRLLESEKQQQQLTIEKEKQIRRLATGLGVLMAVVAVMILVGLISTR
                     RKNQQLAESKRKIEMINADLEQKNVEILSQKEIIEQKNQAITDSIQYAKRIQTAVLPP
                     VDFLSEWGFESFILFRPKDIVSGDFYWGKRKDNKIIVAAADCTGHGVPGAFMSMLGNA
                     FLDEIFNTTEVTDAASVLNLLRDEVITALKQKGVTGEARDGMDISLCIIDRERLKLNF
                     AGANNPLYLVRNSQLIKIPCDKMPIGIHFTELIPFTNQYSDIQLNDVLYLFTDGYADQ
                     FGGEKGRKFMYKQFQALLLNNHHLPMEQQKNVLEKTFDDWKGNYEQVDDVLVIGIRI"
     gene            6749..7468
                     /locus_tag="PLG42_03910"
     CDS             6749..7468
                     /locus_tag="PLG42_03910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU30390.1"
                     /translation="MKFKHLLLLIAGSALILSSCGKKAVSGAKLKDYNDTLSYAFGVN
                     IYYSLLQDSIDLNPLAIARAMVDAKNNKSFMSEDIARAFHYGYLSKKEEEQAARQDEI
                     NKELFSSNIAKGDSFLQANRNKPGVIVTESGLQYKILKLGTGPKPDENDSVKVNYIGT
                     FIDGTKFDSSYDRGEPAEFLVGNVIKGWVEGLQLMPEGSKFVLYIPPDLAYGAGGAGN
                     VIQPFTTLVFEVELLQVKKQQ"
     gene            7488..7868
                     /locus_tag="PLG42_03915"
     CDS             7488..7868
                     /locus_tag="PLG42_03915"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOU30391.1"
                     /translation="MAFEITGKIIDILPVVNVSDRFRKREFVIEKKDAGGGIVFVEYI
                     KFQLVQEKCDIIDDSFLNEEVKVYFNIKGNRWEKDGRVSYFTNLDAWKIEKISARTNE
                     PEAIANLSQENVPPEPEEFSDLPF"
     gene            7956..>9838
                     /locus_tag="PLG42_03920"
     CDS             7956..>9838
                     /locus_tag="PLG42_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU30392.1"
                     /translation="MILFFEVSGNRIYAVDSPEAPGKKEIEKLIWLFGNALLLSRETV
                     DGLFIGPRKEMITPWSTNAVEITQNMGIQGIKRIEEFLPASGNNPEYDPMLQALYRGL
                     DQNIFTIERKPDPIIYIDDIKEYNEKEGLALSDEEIKYLEELSVSIGRKLTDSEIFGF
                     SQVNSEHCRHKIFNGIFIIDGEEMGATLFQLIKNTTHLNRNYVVSAYRDNCAFLKGPV
                     IEQFAPSSQDKPDYFTVKTFESVLSLKAETHNFPTTVEPFNGASTGTGGEIRDRMAGG
                     KGAFPAAGTAVYMTSYPRPDGGRPWEKAIPPRKWLYQSPEDILIKASNGASDFGNKFG
                     QPLICGSVMTFEHIENGKIYGYDKVIMLAGGIGTGKKKDSEKAKPEKGDIIVLLGGDN
                     YRIGMGGGAVSSVETGRYDSSIELNAVQRANPEMQKRVYNAIRALCESDNNPIISIHD
                     HGAGGHLNCLSELVDPAGGKVDISRLPIGDPTLSAREIIGNESQERMGLVLKPRDAET
                     LEKIAGRERAPFYIIGEITGDHRFVFYNSATGEKPMDLDLDALFGKPPRTVIKDTKIT
                     NSFRSISYNPAKIHEYLTMVLQLEEVACKDWLTNKVDRSVTGRIAKQQCAGMLQLPLN
                     NLGA"
BASE COUNT         3137 a         1977 c         2089 g         2635 t
ORIGIN      
        1 catctcggtg atatatgata ctgaagagag gcttattgaa accgaaaatg aatattgtaa
       61 tatagtttca atgattaatg attatactct tgctgagaga tttaatggat tatgattttt
      121 caatagcaca ctgatgacac ggattgaaca gattttcaca gattaataaa tgctatttgt
      181 tgtatctgta taatctttgt gccgataaaa tatagaaact gattcctttt tcataaaaaa
      241 taaaagcagg ttatgttatg tgtaacctgc ttttaataaa tgaatctttt attggttttt
      301 acctaccggt aatggtttct gatcacacta aaatttttgt tgtcagattc gtaatgcttt
      361 tctggattgc accgaagcag tcgccgtcct ttgtacggtc ctgttcaacg atcatgtact
      421 tcatgccagc cgtctgcttt gcggcaatga ttgccttgaa atcaattaat ccctcaccta
      481 cgggtgcgaa atcgtcaaca ccctcggtag taaagaaagg ttcctgcttt gtatacatat
      541 ccttcagatg gaacagttca aacctgccgg gatatttgtt aatgatatcc accgggttct
      601 gacctgcttt tgttacccag aaaacatcaa gttcaagggt tacgtaattc ggatcaagtt
      661 cggccatcat aacatcaaaa taagggatct taccttcaac ggtatcaaat tcgaagttat
      721 ggttatggta tccgaacatt atgccatgat ccttcattat cttaccaacc tggttccagt
      781 cggctgccat tttctggtag ctggcaattg ttgttcttgc ttccggaaca atccatggct
      841 ggatgcagta tttaacaccg agtttggcat ggtcttcagc catctttgtg gcattctcga
      901 gtgttatgcc ctgtgcctca acctgagtat ggctgctgag aatctccatt ccaagatcct
      961 ctaccagttt cctgaactcc tcaggttcat aaccgtaaaa ttttccattt tcataaccgg
     1021 ccaattcaac atatttgtat cccatgtcgg atactctctt cagtgagccg ggaacatcct
     1081 tgaatatctc atcacggatg gtatacagct gaagacctat cccgaaactc tttggatcgg
     1141 gagctgcctt ctttgctcct gtgcccttgc aggcattttg tgtcagcaga aaggcaccag
     1201 ctgctcctgc agcggtcagc ctgataaaat ctcttcttga atgtctgttt ttcataagcg
     1261 gttaaattag ttaaacttta ataaatatct gatttataat tatttaaaca atttcacatt
     1321 tctcctttcc tcaaaaaatc tgtgctttca gtcggggaat aaaattaaaa cattcataat
     1381 taatataaaa atgaaaaaga tgtttttatt ctatctgccg ttatgaaaaa attgacagga
     1441 aaatacctct tttcagaggg ttagggggtg aaatatgtca ggccccctac cagaggcgtt
     1501 gggggtaagt aattgcgaat taattaaaaa aaaggaatag gagcgaaagt ataaatttca
     1561 attatggtac aggaagctca caggaaaagg caggatattt ctgcgtaaat ttttaatacc
     1621 tttaccgtat tattacttgg catgaagaat tcagttctga tattttttct atcactactg
     1681 ctaattccag tcaggtctca ggagccggag agggtatttc ctttcaggct tggggcagaa
     1741 gacctttatc tgcctgtcga cactgttgag atgagtgtta tatcggcaag ccgcagttca
     1801 aagaagatag gtgaattgcc aattaccata catgtcataa cccgtgagga gattatccgc
     1861 aatcattatt atactctgat agatgcaata aagacgttac ctggagttcg cgtttcgcaa
     1921 cccggttcgg gtgagctcgg ggaaagcttc cagctaagag gtctcactgg caacctttat
     1981 accatgatac ttataaacgg tctgcctgta aagcccagtg cagtagtggg tatgcctgtg
     2041 ctggcacagc tgccggtgcg tcaggcagag agaatagaaa taatctatgg cccggccgca
     2101 gcaatatatg gtgccgacgc agtttctggc gtaatcaata ttataacccg tgaagccgaa
     2161 aaaggaactt tcgttctggg agatgtgagc ataggccagg ataatttcag aaacactgac
     2221 tttatggttg gcggtaaggc aggaaaagac aaaaatatac tccagtacag cttttacggt
     2281 ggtttgaacg aaacaggcga tcttaacatt aagaagaaat atgagcagat ctataatcca
     2341 atgcactacc ttcatgaaaa aggttacaga tacctgctcg gctcacaata ttttgatcct
     2401 gctgacataa ccgaacaggt gcttttcaac tatggaatac gcccgactga tttcatgcgc
     2461 gaaaactaca tgataaacta tgatggctca ataaataatc ccgaaatgca ggacctgccc
     2521 ggtatgagtt cccttctggg tttcaacctc aggttcagag gcgtgagcat ttcatacaac
     2581 aacatgtacc ggcggcttca tagctcaatc ggacaatcga cctatctgtt caaatataac
     2641 aatccccaga acttctgggg agaaaatatc agaaccacaa cactcagtta cagcagggac
     2701 tggacttcca gattttcgac cacaacaaac ttctcaaacg tcacttaccg catggacaat
     2761 aactccagta tgggggtgac gtttatcgat tacacagata aagtataccg ttatgctgcc
     2821 ggtagggata tgctattcga acagcttttc accttcatgc cggttaacag tctcgaaata
     2881 atgtcgggta taacttacca gctgtcaggt aacctgccgc aaacaaattt cctggattca
     2941 ccattccggc cgggtgaata taaatacttc agtaaatacg tctatgtaga agattctgtt
     3001 tcaggcagat ttggaataaa cccaatcgtc taccataata tttctgcctt cacgcaaaca
     3061 tactattcta taagttcctt cagatttatg ggaggattgc gattcgacag gaattcactt
     3121 tacggatcaa gtataaatcc caggcttgca gcactctata tatttaacca gcgtacaagt
     3181 ataagagctt cagcaggatt tgcttataaa gcgccaccat cgtcaatgtc ataccagtcg
     3241 ctggcctata aagccggcac aaatcacgat agcctgattt atctaactat ccccaatccc
     3301 aatcttgaac cggaaaaata tatgtcagtc gaacttggtg tggcaagaac aaccagaaaa
     3361 agggtaacgt atgacatatc aatatattac aattctataa gaaatctcat tctgaacaaa
     3421 tatgtcaggc tcagtgaact cagtctaccc cgcgcaatta ttaagcccga caccgctacc
     3481 gttcttgtaa aagagaacga aaagagcgcc gtttccagat tatacggcct tcagataaat
     3541 atcaaaatga atgatattgt aaaatcaata aatcttgatg ccgagctgag tctctcattt
     3601 ggcaaatcga gtcaaacatt tcccgatatt attgagttag ctactaactt tcttggcaat
     3661 ttcaagcttg ttccgaatca tttcggacaa cttaatgttt caatggaacc tgcaaagaat
     3721 ttgtatataa gggtatcaag catatgggaa tcgagctggc ttagggttat tattcctttc
     3781 aaagacattt ataacaatat tttcaaagat tttgacggtt tttacagcat ggatgctgtg
     3841 gctgactaca ggataggaag caatctcagt actttcatta aaatcaataa catttttgat
     3901 gaaaagtatg gtggaccggc ctattcggga atgaatactc ccctacccta cagcccgcag
     3961 cttggaagaa gcatacagat cggattaact tatacgttaa actgatgcgg ttgagaatta
     4021 aatatccaat atatttttac ctgctaattg cgtggcttcc agctttttcg gagagcctcc
     4081 acgggcagga tatcaaaaca accgacacac ttctcctaaa acagagaatg aatgactctc
     4141 tcactacagt taacctgctt cagacagcca taaccatgat aaaagcagga gaagatgaaa
     4201 gtgctctctc agtaatttct aaagccgaag aaattgcatc gtcagacgaa tttttactca
     4261 ttgaagcaga atgccagaaa ctggcaggtg atatttatag ccggaggtct gattcctggg
     4321 aagaaatgct tacgtcttac ctgaaagcga tctccattta taaccgaaca ggtaacaggg
     4381 aaaaagaagc cgaaataact aaaattatag ctgataatta tttcaggaat gggctttaca
     4441 aaaaggctgc cctatggtat cagcaaacat tcgaactcta taaagaactc aaatcattaa
     4501 acctggctgg actggctgaa cttacagcaa ctgcatggta taacctgccc gatgatacac
     4561 tgtcgctaaa atggtacaac acagctcttt catattttga aactgaagga aatgtcgaag
     4621 gtagtataag atgtaccaga aaaattgctt cgctttacgc tgaaacagga aattataaag
     4681 aggctgaaga aaaatacttc ggattgcttg atatttataa aagccgtaat gattctcgaa
     4741 acatatcgct gatatacaat aatctgggag ttattaaata ccgtcagaaa gaactaacgg
     4801 cagctctgaa ctttttcagg gaagctgaaa aagaacaaat taaatctgag ccagattatt
     4861 attttcttac agatgtttcc tcgaacattg ctataacatt acaagctgcg ggcaaccagg
     4921 cagaaatgat gaggaatttc agcagggcac tcgattatgc ccgtaagtcg gagagaaaag
     4981 atgaacaggc aagaatagcc tacctgctat cgctgatata tttcaacaga ggagataatt
     5041 atcatgccga cctttattgc aaggagtgtg tggaatcggc aggtgcgtca ggtgcatatg
     5101 caataatgca ggagtgctac caaacctggt cgggtgtgat ggagaaatcg aatgatttca
     5161 ttaaagctct tgagtattat gagaaatacc tcaacctgag ggattcactt aactttgaaa
     5221 aaaggctggc agagcagaag gaagctgaca ggcagaatta tttcgaccag gttgaacaga
     5281 gaattaagct cgatattgca ggtgaagaaa tcagcaacct tgaaatgaaa actttaaggg
     5341 ctgagtcggc acgaagggag aatgaactta aacttctcct caagcagcag gagcttgacc
     5401 gttcggaaag agagaggctg gcacagtcgc ttgcccttga acgtgaaaga ttccagctta
     5461 acaggaggga gcaggaagtt atggcacttc gacagcaaca gagaatcgac agtctggaac
     5521 ttgtccagaa aagaaacgaa gcactggtac tggagcagaa caaccggcta cttgaatcgg
     5581 aaaaacagca acagcagtta accattgaaa aggagaaaca gataagacgc ctggctacag
     5641 ggctcggtgt tcttatggct gttgttgctg tgatgatttt ggtcggactc atatcgacaa
     5701 ggagaaaaaa ccagcagctg gcagaaagca aacgcaaaat cgaaatgatt aatgctgatc
     5761 ttgagcaaaa gaatgtggaa attctcagtc aaaaagaaat catagagcag aaaaaccagg
     5821 caattactga cagtattcaa tatgcgaaaa ggatacagac tgctgtactt ccgcctgtcg
     5881 attttctttc tgaatggggg tttgaaagct tcatcctgtt tcgccctaaa gacattgtga
     5941 gtggagattt ttactgggga aagagaaaag acaataaaat cattgttgct gctgccgatt
     6001 gcacaggcca cggtgtgcca ggcgcattca tgagcatgct gggcaatgcc ttcctcgacg
     6061 aaatttttaa tactacagaa gtcaccgatg ctgcatcggt cctcaatttg ctgagagacg
     6121 aagtgattac cgctctaaaa caaaaaggag taacaggtga agcaagagat ggaatggata
     6181 tttcactctg cataattgac agggaaaggc tcaaattaaa ctttgccggt gccaataatc
     6241 cgctttacct ggtccgcaac agccagctta taaagatccc ttgcgacaaa atgcctatag
     6301 gcattcattt cacagaactc attccattta caaaccagta ttcggatatc cagcttaacg
     6361 atgtgctgta tctctttacc gacggctatg ctgaccagtt tggaggtgaa aaaggaagaa
     6421 agttcatgta taaacaattt caggcacttc tactgaataa ccatcacctc ccgatggagc
     6481 aacagaaaaa tgttcttgaa aaaacattcg atgattggaa agggaattat gaacaggtcg
     6541 acgacgttct tgtaataggt atcagaatct gaatattctt ttatattcag aaaatccatc
     6601 aacatgatat ttttcattaa atgcttgatt caaaaaacaa tattatcaga aaaatgttcc
     6661 tgaaaaaaat tttaaacatt attttttaat gtctgtaatt cttatccgca ttgtgctatc
     6721 tttgtaaaaa atttttaaat aattaatcat gaaattcaaa catttacttc tgttaattgc
     6781 cgggtcggct ctgattcttt cgtcatgcgg gaaaaaagcc gtttcaggtg caaaattaaa
     6841 agactacaat gatacccttt cgtatgcatt tggagtaaat atttattact ctcttttgca
     6901 ggacagcatt gatttaaatc ctcttgcaat agctagagcg atggtagatg cgaaaaataa
     6961 caaaagcttc atgagtgagg atattgccag ggctttccac tacggatatc tcagcaagaa
     7021 ggaggaagag caggcagccc gacaggatga aatcaacaaa gagctcttca gcagcaacat
     7081 cgcaaagggt gactcttttc ttcaggcaaa cagaaataaa cctggagtta tcgtcacaga
     7141 aagcggactt caatacaaaa ttttaaaact tggaacaggg cctaaacctg atgaaaatga
     7201 cagtgtgaaa gtcaattata ttggaacatt tattgacggc accaagtttg attcatcata
     7261 cgaccgcggc gaacctgctg agttccttgt aggaaacgtt ataaaaggct gggttgaagg
     7321 acttcagctg atgccggaag ggtcaaaatt cgtactctat ataccacccg atcttgcata
     7381 cggtgcaggc ggcgcaggaa atgttatcca gccattcaca actcttgtct ttgaagtcga
     7441 acttttacag gttaaaaaac aacaataaat aaagttctgt accggaaatg gctttcgaaa
     7501 ttaccggaaa aatcatcgac attctgccgg ttgtcaatgt aagtgacaga ttccggaaaa
     7561 gggaatttgt aattgaaaaa aaagatgcag gcggaggcat cgtctttgtt gaatacataa
     7621 aatttcaact tgttcaggaa aagtgcgaca ttattgacga ttcattcctg aatgaggaag
     7681 taaaagtcta tttcaatatt aaaggcaaca ggtgggaaaa agatggaagg gtaagctatt
     7741 ttaccaatct cgacgcatgg aagattgaaa agatctcagc ccggacaaat gagccggaag
     7801 ccatagcgaa cctttctcag gaaaatgtac cacctgaacc ggaagaattc agtgatctgc
     7861 ctttttagtg ctccggcagg catgttatac aataatatta atattgtgta tctgagaaac
     7921 agatacatta ttttcgaata tttgtttctg atcaaatgat tctttttttt gaggtatcag
     7981 gtaacaggat ttacgccgtt gactctcctg aagctccggg aaaaaaggag attgaaaaac
     8041 tcatctggct tttcggaaac gctttattgc tgagccggga aacagtagac ggcttattta
     8101 ttggccccag aaaagagatg ataactccat ggagcaccaa tgcggtggaa attacccaga
     8161 acatgggtat tcaaggaata aaaaggattg aagagttcct gccagcttcc ggcaacaatc
     8221 cggaatatga tcctatgctt caggctctct acagaggact cgaccagaat atattcacaa
     8281 tagaaagaaa acctgaccca atcatttata ttgatgacat aaaggaatac aacgagaagg
     8341 aaggtcttgc actgagtgac gaagaaatca aatatctcga agaattgtcg gtttctatcg
     8401 gacgcaagct tactgacagt gaaatatttg gcttttcaca ggttaattct gagcactgcc
     8461 gtcataaaat tttcaacggc atctttataa tagatggtga ggagatggga gcaacgctgt
     8521 ttcagctaat taaaaacacc acccatctta acagaaatta tgttgtatcg gcttacaggg
     8581 acaactgcgc ctttctgaag ggacccgtga ttgaacaatt tgcaccttca tcgcaggata
     8641 aacccgatta ttttacggta aaaacttttg aatcggttct ttcgcttaag gccgagacac
     8701 acaattttcc tacaacagta gagcctttta acggtgcctc cacgggaaca ggaggtgaga
     8761 taagagacag aatggcaggg ggaaaaggtg cttttcccgc cgcaggtact gccgtttaca
     8821 tgacctcata tcctcgtcct gacggaggaa ggccatggga gaaagccata cctccaagga
     8881 aatggctcta ccagtctccg gaagatatat tgataaaagc atccaacggc gccagcgatt
     8941 ttggaaataa attcggacag ccattgatat gtggttcggt aatgactttc gaacacatag
     9001 aaaacggaaa gatatacggt tatgataaag taataatgct tgccggaggt atcggaaccg
     9061 gaaagaagaa agacagtgaa aaagccaaac ccgagaaggg cgacataata gtgcttcttg
     9121 gtggtgacaa ttaccgaatc ggcatgggag gtggagctgt ttcctcggtc gagactggca
     9181 gatacgactc ttccatcgaa ctgaacgccg tacagagagc aaaccctgaa atgcagaaaa
     9241 gagtatataa tgccatccgg gccctttgtg aatcggataa caatccgata atttcaattc
     9301 acgatcatgg tgccggaggg catctgaact gcctgtcgga acttgtcgac cctgcaggtg
     9361 gaaaagtgga catcagtaga ctcccaattg gtgacccaac actttctgcc agagaaataa
     9421 tcggcaacga atcgcaggaa agaatgggac ttgtactgaa gccacgagat gcagaaacac
     9481 ttgaaaaaat agccggaaga gaaagagccc ctttctatat catcggagaa ataaccggag
     9541 accacaggtt tgtattctac aactcagcaa cgggtgaaaa gccgatggat cttgacctag
     9601 atgctctctt cgggaagcct cccagaacag taattaaaga cacaaaaatt accaacagtt
     9661 tcaggagtat atcctataac cctgcgaaga ttcatgaata ccttaccatg gttcttcagc
     9721 tcgaagaggt tgcatgcaaa gactggctta ctaacaaagt tgaccgttca gttacaggcc
     9781 gtatagcaaa gcagcagtgt gcaggaatgc ttcagttacc gctcaacaac cttggagc
//
LOCUS       DAOSNB010000058         9782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6935_length_9782_cov_4.429937, whole genome shotgun sequence.
ACCESSION   DAOSNB010000058 DAOSNB010000000
VERSION     DAOSNB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6935_length_9782_cov_4.429937"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLG42_03925"
     CDS             complement(<1..146)
                     /locus_tag="PLG42_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOU30393.1"
                     /translation="MHKSGFVNILGNPNVGKSTLMNAMVGQKLSIVSPKVQTTRHRIM
                     GILNG"
     gene            complement(158..946)
                     /locus_tag="PLG42_03930"
     CDS             complement(158..946)
                     /locus_tag="PLG42_03930"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOU30394.1"
                     /translation="MPQKNIKGKRSGSRKEVFSKGKESEELMRLNRFIAASGLCSRRE
                     ADELILNGLITVNGKQIKELGYRVRRDDDVRYKNKRLTPEKKVYILMNKPKGYVTTTS
                     DPGGAPTVMDLLGNKIEARVYPVGRLDKNTTGVLLLTNDGELAGRLTHPRFEKKKVYH
                     VVLDRDVAKSDLEKLVKGVDIGDETVYADAAAYPSPDDKSHVGIEIHSGQNRVIKRMF
                     EKLGYKVKNLDRVYFAGLTKKNLPRGKWRFLTEKEINMLKRGIF"
     gene            1043..2425
                     /gene="glmM"
                     /locus_tag="PLG42_03935"
     CDS             1043..2425
                     /gene="glmM"
                     /locus_tag="PLG42_03935"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOU30395.1"
                     /translation="MALIKSISGIRGTIGGRQGENLTPVDIVMFTAAFGQWVRKDSGK
                     EKVKIVTGRDARQSGKMIADIVNATLQAMGIDVIDIGLASTPTVEIAVQGTGANGGII
                     ITASHNPEQWNALKLLNRKGEFLSESDGKEVINISQKNDFVFADSKSAGRITRDETWA
                     QRHTDLIMNLKLVDSKLIASAGFKVVVDCINSVGSMIIPGLLKKLGVKEYILINEVPD
                     GKFSHNPEPLPENLIQLSEKVKTTGADVGFAVDPDVDRLAIVCENGEMFGEEYTLVAV
                     ADYVLSHTPGNTVSNMSSTRALRDVTLKYGGKHFTSAVGEVNVIEEMKEREAVIGGEG
                     NGGVIYPELHYGRDALVGIALFLTHLASQKTTCSELRKRYPDYFLVKTKIAREKDFNI
                     EKIRKVAKDIFPDSSTDERDGILVESNEGWVHARTSNTEPIIRVYAEAKSPEKAAELA
                     EKMARIVKNV"
     gene            2617..4173
                     /locus_tag="PLG42_03940"
     CDS             2617..4173
                     /locus_tag="PLG42_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU30396.1"
                     /translation="MKKTLIISGIVVAVIIIAVVVISRLSHKSDLSVLFAEAKKGQFD
                     IIVYTTGELQAENSVFIRGPEIAQLRNIRGMEIRIQDLVPEGTIVKEGDYVATLDRTS
                     FDNTLKDELERLATYETNLETAILDTAVTLTNLRDNIKNLTYNVEEARITLEQSKYEP
                     PATIRQAEINLDKAERALEQAKRSYALRVEQTKANILNTRRLLNEQRQKVADLQDLLS
                     KFIVRAPADGMVIYYRDRTGAKRKTGSSISPFDNIIATLPDMSSMISKTYVNEIDVSK
                     VKAGQRVEITVDAFPDKRYTGVVTSVANIGEQLPNADAKVFEVIIKLDVTDPILRPAM
                     TTGNKIVTKTLNDVVYIPLECVQTGADSIPFVYTKSGKKQIVLLGESNENDVVVEQGI
                     EPGTELYLSTPEKPEKFTLAGEDLIPIIRERQRQKEEEERRLREEAARAAERRTGMGF
                     RSGGARGQFPGFTPGEFQGAGAGFPGGVRDTAAMRRRMQEMRSSRESGGGAVRDTTAR
                     RRMNPQSGGQ"
     gene            4226..5611
                     /locus_tag="PLG42_03945"
     CDS             4226..5611
                     /locus_tag="PLG42_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU30397.1"
                     /translation="MFRFILRYFHDIQIAMESIVSNKLKSILTALGIIFGVAAVIAML
                     AIGRGAKQEILEQMKMVGVNNILINPVIQEKTSTSEEGEKQQRKFSRGLTMLDVEAIR
                     THIPSVKRISPEISFNSTAIFNGVKHTAKLVGVSNDYFYLYNLPLVAGTVFNDYQNEN
                     GVQVCIIGANIRSKFFSKIDPIGQYIKFNGIWLKVIGVLQKTNVSLTGFEEKGVNVYN
                     DNIYIPIQTMLMRFQNRALVNTKLASEASTTAFNIMGGGMGFMARVIVSSSDANVQQE
                     TNYNQLDRIVVQVNETEQLNTTTEVLSRMLLRRHSNVKDFEITVPELLLKQQQRTKDI
                     FNIVLGAIASISLIVGGIGIMNIMFASVMERIKEIGTRMAIGAKKMDIVVQFLSEAVL
                     ISISGGFIGIFLGVIMAKIIEKLAGIMTIVSFFSVFIAFGVSVAVGVIFGYSPAKRAS
                     EKDPIESLRYE"
     gene            5625..7115
                     /locus_tag="PLG42_03950"
     CDS             5625..7115
                     /locus_tag="PLG42_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU30398.1"
                     /translation="MNRILVLLFTGFLFASFTVRTEAQAVKKVMTLDEVIKVAEDQSP
                     SALIAKHRFRASYWQYRSFKAQYLPSLTLTGTVPDFFNGLERVWDSRDTAWKYFATNT
                     VSSTGSLSLSQAIGPLGTVISLSSDLTLYKDIEKNLPVNYITNPVSININQPIRRYNT
                     LKWQAKIEPLLYEMAKKEYLNTIEEVHMNAVQNFFMLALAQINKQIAEMNYSNADTLY
                     RIAQGRYQLGTIAEDDLLQMQLSFLNAETARKQAEMNLRDRQIRLASFLGYNENVEIE
                     LIIPSEIPNLQVDTQEVLELALKNNPDIINQRINTLNAQSSLAQARTNRGLTATMIAS
                     IGFKQQNTDFLQAYQNLSQSQRVRIGLTIPILDWGQAKGRYKMALSSLELAEVQEKQA
                     MSNFQQNLFLDVAQFNLQAEQVAIAAKSDTVAMRRYEVTKARFLIGKISVLDLNDADA
                     RKDTNRRAYIQALQQYWTYFYNIRALTLYDFINRKPIEVDFEKLLL"
     gene            7265..>9782
                     /gene="secDF"
                     /locus_tag="PLG42_03955"
     CDS             7265..>9782
                     /gene="secDF"
                     /locus_tag="PLG42_03955"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOU30399.1"
                     /translation="MQNKGLIIVLAVTLAIVSLYQLSFTAATYKVKSDARKYAKGDLV
                     KEVAYIDSMSTLSKDQWSFLGYTFKECLKKELNLGLDLKGGMNVILEVSVEDILRALS
                     NYNPDKTFNEALARARQRQLQTQGDFLTLFANAFTELDPQARLASIFGTVEMKDKINF
                     NSTNEEVIKVLEKEIDNAINNAFNILRTRIDRFGVTQPNISQLSTKGRILIELPGVNN
                     PQRVRELLSGTANLEFWETYENSEMIGYLVQANNLLREILANTQKQADSLKLASESRA
                     STQQDTTRKGEESILELIKKDTTKTAAEAETLEQFTAQNPLFGILRPHVDQNGQPYQG
                     SLIGLASFRDTATVNKYLKMNQIRALFPRDVRFYWSQEPYKYDDTKTFYELHAIKVTT
                     RDGRAPLDGNVITSARPSTGVIASDIKVEMSMNAEGAKTWARLTRENIGRCIAVVLDG
                     YVRSWPRVSTEITGGNSEITGNFTLEEAQDLANILLSGKLPAPARIVSDTVVGPTLGK
                     EAIKSGVTSFAIAFLLVLAYMIFYYSHKAGVVTDIALLANVFLLFGVLASLNAVLTLP
                     GIAGIVLTMGMAVDANVLIYERIREELRAGKGLKLAVADGYRRAYSAIIDSNVTTLLT
                     GIVLYVFGTGPIRGFATTLVIGIVTSLFTAIFITRLIFEAQLKRNKELTFSIPLTANL
                     LKNTKIDFIGARKYFYALSAVIVIAGIVSLFARGLNMGIDFTGGRTFVVRFDEPVKTA
                     DVAQRLNSVFGQMPQVVTFGSENQVKITTKYKINEPGAEDEVETLLYQGLKDMVGGNL
                     SKEEFLLKYRVSSETIGPTIASDIKIKAFYSVGIALVIMFF"
BASE COUNT         3022 a         1828 c         2164 g         2768 t
ORIGIN      
        1 ccgttgagga tacccattat gcggtggcgt gttgtctgta cctttggaga gacaatagaa
       61 agtttttgcc ccaccattgc attcatgagg gttgatttac cgacattcgg attacccaga
      121 atatttacaa atcctgattt atgcatttat taatttttta aaagatgcct cttttaagca
      181 tatttatttc tttttcagta agaaacctcc attttccacg cggcaggttt ttctttgtaa
      241 ggccggcaaa atagactctg tcgaggtttt taaccttata tccaagcttt tcgaacattc
      301 tttttatgac ccggttctgg cctgaatgaa tttcgattcc tacatggctt ttatcgtctg
      361 gtgaagggta tgcggctgcg tcggcataaa ctgtttcatc tccaatatcc actcctttta
      421 caagtttctc aaggtcgctt ttggcaacat ctctgtcgag aactacatga tagactttct
      481 ttttttcaaa acgtggatgg gttagtctcc ctgccagttc tccgtcgtta gtcaacagaa
      541 gcactcctgt tgtattttta tcaagccttc caaccgggta aaccctggct tcgattttat
      601 tacctaaaag gtccataacc gttggtgctc cgcctggatc tgatgttgtt gtaacataac
      661 cttttggttt gttcataaga atatacacct ttttttctgg tgtgagcctt ttgttcttat
      721 atctgacgtc atcatccctt cttaccctgt agccaagttc ttttatctgt tttccgttta
      781 ctgtaatcaa tccatttaat atgagttcat ctgcttcgcg ccgtgaacac aagccgctgg
      841 cagcaataaa cctgttcaag cgcatcagtt cttcagattc tttccctttt gaaaaaactt
      901 cttttcttga tccggatctc tttcctttta tgtttttttg tggcattttt tataaatcag
      961 ggtgcaaatt tacaattaat tagcgagata atactaaata ctttattttt gcatttttac
     1021 aattgcaaaa ctgtttttga aaatggcgct tataaaatca atttcaggaa ttcgtgggac
     1081 gattggcggc aggcaggggg agaatctgac gccggttgat attgtaatgt ttacagctgc
     1141 attcggacaa tgggtcagga aggattcagg aaaagaaaag gttaaaattg taacgggtcg
     1201 tgatgcaagg caatcgggga aaatgattgc cgacatcgta aacgccacgc ttcaagcaat
     1261 gggtattgat gttattgata tcggtctggc ttcaacgccc accgttgaaa ttgctgttca
     1321 gggaaccggg gctaatggag gtattataat tacggccagt cataatccag aacagtggaa
     1381 tgcacttaaa ttgctgaacc gaaaaggcga attcctttcc gaaagtgatg gtaaagaggt
     1441 aattaatata tctcaaaaga atgattttgt ttttgctgac agtaaaagcg caggcaggat
     1501 aaccagggat gaaacatggg cacaacgaca tacggattta ataatgaatc tgaaactggt
     1561 tgatagtaaa ttgattgcat cggcaggttt taaagtagtg gttgactgta taaattctgt
     1621 tggcagcatg attattcccg gattactcaa gaaattgggt gtgaaggaat atattctcat
     1681 caatgaggtc cctgacggaa aattttcaca taaccctgag ccgttgccgg aaaatcttat
     1741 tcagctttcg gagaaagtta agactacagg tgctgatgta ggttttgcag tcgatccgga
     1801 tgttgaccgc cttgcaatag tatgtgagaa tggtgagatg tttggcgagg aatatactct
     1861 tgtggctgtg gctgattatg ttctttcaca tactccggga aataccgttt caaatatgtc
     1921 atcaacaagg gcattgagag atgtaacttt aaaatatggt gggaaacact ttacttctgc
     1981 tgtgggtgag gttaatgtta tagaggaaat gaaagaaagg gaagctgtga taggaggtga
     2041 aggtaacgga ggggtaattt atcctgaact tcactacggg cgagatgctc ttgtaggtat
     2101 agcattgttc cttacacatc ttgcatcgca aaaaactaca tgttcggaac tgagaaaaag
     2161 atatccagat tattttcttg ttaaaacaaa gattgcccgt gaaaaagatt ttaatattga
     2221 aaagatcaga aaggtagcta aagatatatt tccggattca tcaaccgatg agcgtgatgg
     2281 catccttgtg gaatcgaatg aagggtgggt tcatgcaaga acgtcaaata cagagcctat
     2341 tatcagggtt tatgcagaag caaaaagccc tgaaaaagca gcagaacttg ccgaaaaaat
     2401 ggctcgtatt gttaaaaatg tttaaaataa gaaattttgt ttagtcagtc aaggaataaa
     2461 agcacttgtt tatgtgtctt ataatatgaa acaaaaaagg ttattgctgt taattattgt
     2521 taatgaataa tattacctta cttttattaa tacttttgtg gctgaaaaat atgacaatac
     2581 aataaattta aaacaaataa aaacagatat ttcacaatga aaaaaacatt aattatttct
     2641 ggaattgttg tggcagtgat aattatcgca gtggtagtta tcagcaggtt atcccataaa
     2701 agtgacctca gtgttttatt tgcagaggca aagaagggcc agtttgatat aattgtatac
     2761 actacaggag aacttcaggc cgaaaactca gttttcatca gggggcctga aattgctcag
     2821 ctccgtaata taaggggtat ggaaatcagg atacaggatc ttgttcctga aggcactatt
     2881 gttaaggaag gagattatgt ggcaacactt gacaggacat cttttgataa tactttaaag
     2941 gatgaacttg agaggcttgc aacatatgag acgaatcttg agactgctat tcttgatact
     3001 gcagtgactc ttacaaattt aagggataat atcaagaacc tgacatataa tgttgaagag
     3061 gcccgcataa cacttgaaca gtcgaaatac gagcctcctg ccaccatccg tcaggcagag
     3121 atcaatcttg acaaggctga acgtgcgctc gaacaggcaa agaggagcta tgccctcaga
     3181 gttgaacaga caaaagccaa tattctaaat acaaggcgtt tgcttaatga gcagcggcag
     3241 aaggtagccg accttcagga tctgctttca aaatttattg tcagagcgcc ggccgatggt
     3301 atggttattt actacaggga tagaaccggt gccaagagaa aaacaggctc ctcaataagt
     3361 ccattcgaca atataattgc aacacttcct gatatgtcgt cgatgatatc gaaaacatac
     3421 gttaacgaga ttgatgtcag caaggtaaaa gccgggcaga gagtagaaat tactgttgat
     3481 gcatttcctg acaagaggta tacaggtgtg gtcacaagtg ttgcaaatat tggtgagcag
     3541 cttccgaacg ctgatgccaa ggtgtttgag gtgattataa aactcgatgt tactgatcct
     3601 atacttcgtc cggcaatgac aacaggtaac aagattgtta ccaagactct gaatgatgtt
     3661 gtatatatac cgctggaatg tgttcaaacc ggtgccgaca gtataccttt tgtttataca
     3721 aagtcgggaa agaaacagat tgttctgctg ggtgaatcga atgagaatga tgttgtggtt
     3781 gagcagggta ttgagcctgg aacggaactc tacctgagta cgccggagaa accggagaaa
     3841 tttactcttg caggagagga tttaatacca atcatcaggg aacgccagag acagaaggag
     3901 gaagaggaga gacgtttgag ggaagaggca gccagggctg ctgaaagaag gactggaatg
     3961 ggattcagat caggcggtgc cagaggtcaa ttcccgggct ttacgcctgg tgaattccag
     4021 ggtgcaggag ccggattccc cggtggagtg cgtgatactg cagccatgag acgcaggatg
     4081 caggaaatga gaagttcaag agagagcggc ggaggtgctg taagagatac taccgcacgc
     4141 agaagaatga acccacagag cggtggacaa taattaatcc cgcctctgtc attttcctga
     4201 aattaatcgt aaaaaacatt aaaagatgtt cagatttata ttaagatatt ttcatgatat
     4261 tcagattgct atggaatcga ttgtctcaaa caaacttaaa tcgattctca cagcactcgg
     4321 aatcattttc ggtgtggcag cagtcatagc aatgcttgcc attggccgcg gggcaaaaca
     4381 ggaaatcctc gaacagatga aaatggttgg tgtaaacaat attcttataa atcctgtaat
     4441 acaggaaaag acatcaacct ccgaagaagg cgagaaacag cagagaaaat tttcacgggg
     4501 acttacaatg ctcgacgttg aggcaatacg aacacatata ccgtcagtta agaggatcag
     4561 tccagaaatc tcatttaact ctacggcaat ctttaatggc gttaaacaca cagccaaact
     4621 ggtgggggta tcgaacgatt acttttatct ttacaacctg ccactggttg ctgggacggt
     4681 attcaacgac tatcagaatg aaaacggagt acaggtatgt attataggag ccaacatccg
     4741 ttctaagttt ttcagcaaaa ttgatccgat aggccagtat attaagttca acggtatctg
     4801 gcttaaagtg attggagtat tgcagaaaac aaatgtttct cttacaggat ttgaagaaaa
     4861 aggtgtaaat gtttataatg acaatattta tatacccatt cagacgatgc tgatgcggtt
     4921 ccagaacagg gctcttgtaa atacaaaact tgcatcagaa gcttcaacca cagccttcaa
     4981 tatcatggga ggcggaatgg gatttatggc aagggtgata gtcagttctt cagatgccaa
     5041 tgtacaacag gaaacaaatt ataaccagct cgacagaatt gttgttcagg ttaatgaaac
     5101 agagcagcta aatacaacaa ctgaagtttt aagccggatg cttctgagaa ggcattctaa
     5161 cgtcaaagat tttgaaataa cagttccgga actgcttctc aaacagcagc agagaacaaa
     5221 ggatatattt aatatagtac tcggtgcaat agccagtatt tcacttattg tgggcggaat
     5281 cggaataatg aatattatgt ttgcctcagt aatggaacgc attaaggaaa ttggaacgcg
     5341 tatggcaata ggagccaaga aaatggatat tgtggttcaa tttctttcag aggcagtcct
     5401 tattagcata tcaggtggtt ttattggaat ctttctcgga gtgataatgg caaagattat
     5461 tgagaaactt gctggtatta tgacaatagt atcattcttt tcagttttta tagctttcgg
     5521 ggtatctgtt gcggtaggtg tgattttcgg ttactcgcca gcaaagaggg cttcagaaaa
     5581 agaccccatt gaatcactca gatacgaata aacattttag acatatgaac aggattttag
     5641 ttttattatt tacgggattt ttatttgctt ctttcacagt cagaactgaa gcacaggccg
     5701 taaaaaaggt tatgacactc gatgaagtta ttaaagttgc cgaggatcaa tcgccttcag
     5761 ctcttattgc aaaacacaga ttccgtgcaa gctactggca gtacaggtca tttaaagctc
     5821 agtatctgcc ttcactgacg ttaacaggta cagttccaga ttttttcaac gggcttgaaa
     5881 gagtgtggga ttcaagagac acagcatgga aatattttgc caccaatact gtcagcagca
     5941 ctggatcact ttccctgtcg caggctatag gtccacttgg tactgtgatt tcgttaagtt
     6001 cagaccttac cttatataaa gacattgaga aaaatttgcc tgttaattac atcacaaatc
     6061 ctgtttccat taatataaac cagccgataa ggcgttacaa tacactcaaa tggcaggcaa
     6121 aaattgaacc actcctgtat gaaatggcaa aaaaggaata tctgaacact attgaggagg
     6181 ttcacatgaa tgcggtgcag aacttcttta tgcttgcact tgcccagatt aacaagcaga
     6241 tagctgagat gaactattca aacgctgata ctctttatcg tattgcccag ggaagatatc
     6301 agctcgggac catcgctgaa gacgatcttc ttcaaatgca gctctcattc cttaatgccg
     6361 aaacggcaag gaaacaggca gaaatgaacc ttcgcgacag gcaaattcgt ctggcttcat
     6421 ttctaggtta taatgaaaat gttgaaattg aacttataat tccatctgaa attcctaatc
     6481 tgcaggtcga tacccaggaa gttcttgaac tggcactgaa aaataaccca gatattatta
     6541 atcaaagaat caatacattg aatgcccaga gcagtcttgc gcaggcaagg actaaccggg
     6601 gactgacagc tacgatgatt gcatcaatag gtttcaaaca gcagaatact gattttctgc
     6661 aggcatatca gaatcttagt cagtcacaga gagttaggat agggcttact ataccgatac
     6721 tcgactgggg ccaggcaaag gggaggtaca agatggcact ttcgagcctt gagcttgctg
     6781 aagttcagga gaaacaggca atgtcgaatt tccagcagaa cctgtttctt gatgtagccc
     6841 agtttaatct tcaggccgaa caggttgcaa ttgctgccaa atctgataca gtagctatga
     6901 ggaggtatga agtgaccaag gcgagattcc ttatcggaaa aatatctgtt cttgatttga
     6961 acgatgccga cgccagaaag gacacaaacc gcagagcata tatccaggcc ctgcaacaat
     7021 actggactta tttctacaac atcagagccc tgacgcttta cgattttatt aacagaaaac
     7081 ctattgaagt ggatttcgaa aaactactgc tttaaagaat tgtgtaatga acttgttgaa
     7141 aggctgcatt gagaggcagc ctttcttatt ttttaattgt ttcttttttg aaaatctttt
     7201 gtttaaattt gcggctcatt atttgattga attaatatta tagaataaca taaaaaattg
     7261 acagatgcag aataaaggtc tgattatagt tctggcagtt acgcttgcta tagtttcact
     7321 ctaccagctg tcgtttacag ccgctacata caaggttaaa agtgatgcca gaaaatatgc
     7381 aaaaggagat ctggtaaagg aagtggcata tatcgattcg atgtcgaccc tgtcgaaaga
     7441 ccagtggagt tttctggggt acaccttcaa ggaatgcctg aagaaagagc tgaatctcgg
     7501 acttgacctt aaaggtggta tgaatgtgat acttgaggta tcagttgaag atattttaag
     7561 agcactatca aattacaacc cggataaaac ctttaatgaa gctcttgcca gggctcgtca
     7621 gcgtcaattg cagacacagg gagattttct cacacttttt gcaaatgcat ttactgaact
     7681 tgaccctcag gcaaggctag cttccatttt cggcactgtt gaaatgaagg ataagataaa
     7741 ctttaattca acaaatgaag aagttataaa agttcttgaa aaagagatag ataacgctat
     7801 aaataatgcc tttaacatac tccgtacaag aatcgaccgg tttggagtaa cacaacccaa
     7861 tatatctcag ttatctacaa aggggagaat tctgatagag ctcccagggg ttaacaatcc
     7921 gcaaagagta agagagcttc tgtcgggtac agcaaacctt gaattctggg agacatatga
     7981 gaacagtgaa atgataggct atctggttca ggcaaataat ctgctaaggg aaattcttgc
     8041 aaatacgcag aaacaggcag attctttgaa actggcttct gaatcacgtg cgtcgaccca
     8101 gcaggatact acccgtaaag gtgaagaatc aatacttgaa ctaattaaaa aagatacaac
     8161 aaaaacagct gctgaagcag aaacgctgga acagtttaca gcccagaatc ctcttttcgg
     8221 gatattaagg ccgcatgttg atcagaatgg acagccatat caggggagcc ttataggtct
     8281 ggccagtttc agagatacgg ctacagtaaa taagtatctg aagatgaacc agatcagggc
     8341 tctgtttccc cgtgatgtcc gcttctactg gagtcaggag ccatacaagt atgatgacac
     8401 aaagactttc tatgagctac atgcaataaa agttaccaca cgcgacggcc gggcacctct
     8461 cgacggaaac gtaattacat cggccagacc ttcaaccgga gttattgctt ccgacattaa
     8521 agtcgaaatg tcgatgaatg ccgaaggtgc caaaacatgg gcacgcctta cacgcgaaaa
     8581 tattggacgc tgtatagcag ttgttctcga tgggtatgta cggtcatggc caagagtctc
     8641 aactgaaatt accggtggaa actctgaaat aacaggtaat ttcactcttg aagaggcaca
     8701 ggacctcgca aacattcttc tttccggtaa actgcccgct cctgcccgca tagtatctga
     8761 tacagttgtt ggtcctacac ttgggaaaga agcaataaaa tcgggcgtta catcatttgc
     8821 tatcgcattt ctcctggttc tggcatatat gattttctat tacagccata aggctggagt
     8881 tgttacagat attgctcttc ttgcaaacgt atttcttctc ttcggcgttc ttgcttccct
     8941 caatgcggtg ctgacccttc cgggtatagc aggtattgtg cttacaatgg gtatggcagt
     9001 tgacgcaaac gtgcttattt atgaacgaat acgcgaggag cttcgtgccg ggaaaggact
     9061 taaactcgca gtagctgacg gctaccgaag agcatattca gcaataatag actctaacgt
     9121 aacaacactg cttaccggta ttgtcctata tgttttcggt acaggcccga taagaggatt
     9181 tgcaacaacg ctcgtcatag gtatagtaac atcactcttt acagctattt tcataaccag
     9241 acttattttt gaagctcaac ttaagagaaa caaagaactt actttttcaa ttccgcttac
     9301 tgcaaacctt cttaaaaaca caaaaatcga ctttatcggg gcaagaaagt atttttatgc
     9361 attatcagct gtaatcgtta ttgctggtat agtttctttg tttgccaggg gacttaatat
     9421 gggaattgat ttcacaggtg gaagaacttt tgttgtaagg tttgacgaac ctgtaaagac
     9481 ggcggatgtt gcccagcggc ttaattcagt ttttgggcaa atgccacaag ttgtaacttt
     9541 tggcagtgaa aatcaggtca agatcactac caaatataaa ataaatgaac ccggagcaga
     9601 ggatgaagta gagacattgc tctatcaggg acttaaagat atggttggag ggaatctgtc
     9661 aaaagaagaa tttcttttaa aatacagggt tagctccgaa accataggcc cgaccattgc
     9721 tagcgatatt aaaattaagg cattctattc tgttggtatc gcccttgtta ttatgttctt
     9781 tt
//
LOCUS       DAOSNB010000059         9767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_6946_length_9767_cov_3.997632, whole genome shotgun sequence.
ACCESSION   DAOSNB010000059 DAOSNB010000000
VERSION     DAOSNB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_9767_cov_3.997632"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLG42_03960"
     CDS             <1..187
                     /locus_tag="PLG42_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HOU30400.1"
                     /translation="LFFRVVKVAFNQRRKILRNSIRASFRLLSEDNPVLSLRPEQLAV
                     QQFVDLTGWIAENALLE"
     gene            complement(179..1459)
                     /locus_tag="PLG42_03965"
     CDS             complement(179..1459)
                     /locus_tag="PLG42_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU30401.1"
                     /translation="MQEKHKKTAAGTLLQLIYEGISFAFSSIYVNKLRTFLSLFGITI
                     GIFCIISVFTVLDWMEKSIRDSISTLGDNVIYVQKFPWSFDPDLNWWDIIRWPAVSVE
                     DFEAITRKSTKSQSASFVVFQPATIKYNKNSISEALVGAATYEIKDIRSFEIEKGRYF
                     SLYEASSGKNVAIIGAEVAERLFEKSNPVGKEITIGQHRATVIGVIKKEGKGGISDSG
                     MDEITIVPLNFGKTFINLRRRNAETQLMIKARPGVAIEELSDEAVMILRAARRLRPGE
                     INNFSVNRASLLTEGFKAVFAGINAGGWFIGGFAILVGGFGVANIMFVSVKERTNIIG
                     IQKALGAKRSFILLQFLTESVILSVLGGLLGILFVFIGTLGINYLYELNMFLTVKNIL
                     LAIFISGTIGVVAGYAPAYTAARMNPVEAIGYSF"
     gene            1708..3234
                     /gene="purH"
                     /locus_tag="PLG42_03970"
     CDS             1708..3234
                     /gene="purH"
                     /locus_tag="PLG42_03970"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOU30402.1"
                     /translation="MMNDKKIKSALVSVYYKEGLDEILKILNELGVRIISTGGTYSYI
                     KGLGIEAETVESLTTYPSILGGRVKTLHPVVFGGILARRGNAEDVGQMKSYNIPEIDL
                     VIVDLYPFEETVARSAVEEEIIEKIDIGGVSLLRAAAKNFNDVLVVSSRSQYGRLASL
                     LKEKGGYTSIEERKLFAAEAFKVTSGYDTAIFRYFNREANLTAFRESFDSFMSLRYGE
                     NPHQKGVFFGDFEMMFDKLHGKEISFNNLLDVDSAIGLIDEFAETTVAIIKHNNACGV
                     ATRDNLMDAWKDALACDPVSAFGGIIVTNRLVDEVTATEIDNLFFEILIAPGYSDSAL
                     EILKRKKNRIILLRKSSDVSPFVYRSILNGVLWQERDMHTEDVADMKPVTERVPDSRE
                     YEDLVFANKVVKHTKSNAIVLARNKQLCASGTGQTSRVDALRQAVEKAGMFGFDLKGA
                     VMASDAFFPFPDSVEIAHKAGITAVVQPGGSVRDQESIDFCNKHGMAMVFTGIRHFKH
                     "
     gene            3247..4269
                     /locus_tag="PLG42_03975"
     CDS             3247..4269
                     /locus_tag="PLG42_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOU30403.1"
                     /translation="MGLFSFLTQEIAIDLGTANTVIIHNDKVVVDEPSIVAIDRNTGK
                     LIAIGEQARQMHGKTHENIVTIRPLRDGVIADFNAAELMIRGMIKMINKGSKLFTPSL
                     RMLVGIPSGSTEVEIRAVRDSSEHAGGRDVYLIHEPMAAAIGIGLDVEAPEGSMVVDI
                     GGGTTEIAVIALGGIVCSKSIRIAGDSFTSDIQAYMRHQHNIKVGEKTAEDIKIAVGA
                     ALPDIDNPPPDFIVRGPNIMTSLPIEVPVSYQEIAYCLDKSLSKIEAAVLNVLEQTPP
                     ELYADIVNKGIFLTGGSSLLRGLDKRLTDKININFHVVEDPLRAVARGTAVALKNIDK
                     FQFLMR"
     gene            4283..5104
                     /gene="mreC"
                     /locus_tag="PLG42_03980"
     CDS             4283..5104
                     /gene="mreC"
                     /locus_tag="PLG42_03980"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOU30404.1"
                     /translation="MRNLLLFIVKYNRIILFILLEMIAVYLISNSYRYHNSQLIKGVR
                     KLTLAWEGVITDTKNYLRIKKINDDLQAENTALWNLIEKNIIKTDSSFLQINDTASGA
                     QYEVLPASVVNNSVNRIKNYFTIDRGKDDGVDVNMGVISPWGVAGITVASSNNYSIVM
                     PVINTDFRLSSKIKSNDYFGSLIWDGKDNRYAVLNDIPQHVALNEGDTIVTTGYSAIF
                     PPGIMVGTISEFKKSGSDFYSIKVRIATDFRKLTYVKVIRNKRQEEQLNLEKGFQ"
     gene            5101..5607
                     /locus_tag="PLG42_03985"
     CDS             5101..5607
                     /locus_tag="PLG42_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30405.1"
                     /translation="MINQLLRYTIIYIVLLLLQVFVFNNIQFSGYVNPYIYILFVLLL
                     PYEISGWLLLIISFFAGFFIDIFMGTAGMHAGATVAAGFARPYILRAISPRDGYVADR
                     ELSMVAYGFNWFFFYALLVVAVHHLVLFYLEIFSFEGFFRTFLKVIFSTVFTVIFIIF
                     AEYIRKGR"
     gene            5617..7440
                     /gene="mrdA"
                     /locus_tag="PLG42_03990"
     CDS             5617..7440
                     /gene="mrdA"
                     /locus_tag="PLG42_03990"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOU30406.1"
                     /translation="MKDLYVNRRIIVAALIIIPAVILLARLFQIQVVETSYRISAENN
                     VLRYVTQYPARGLIYDRNRKLLVFNQPAYDLMVIPGQVSKIDTGEFCKSLNITREYFR
                     ERMKAAASYSKRVPSIFMKQISSEIYAGFQEKLFMFPGFYVQTRTLRKYSKPIAAHVL
                     GYVSEVDENVIKNNPYYKPGDYIGKTGIEEAYEAELRGKKGVKIFLVDVYGRVKGSYE
                     EGRLDTAAVQGKDIVCTIDSDLQEYAETLMKNKSGSIVAIEPATGEILTLVSSPTYDP
                     SLLVGRVRSENFAKLQADTTMPIFNRAIMASYPPGSTFKTVNGLIALQEKVISPSTIF
                     GCNPGYLFVGCHIHESPLDLVGAIKNSCNAYFCQAFRRILENPAYKSVSDAYEQWRNY
                     LLQFGIGRSLGSDLVNEKSGILPTVSFYDSRYGKGRWKALTVISLAIGQGEIGVSPLQ
                     MANMTAAIANRGFYYNPHIVKAIGDSVNVTRGLAEKHTIEIDSSNFEWIIKGMEEAVN
                     GGTGATARIAAIDGITVCGKTGTAQNPHGKDHSIFIAFAPKEKPQIAIAVYVENAGFG
                     ATYAAPVARLLIEKYLKGTITNKSLEDYILNLNLMNGWREE"
     gene            7425..8849
                     /gene="rodA"
                     /locus_tag="PLG42_03995"
     CDS             7425..8849
                     /gene="rodA"
                     /locus_tag="PLG42_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOU30407.1"
                     /translation="MERRVNIWRAVDKITIVIFLLLVILGWLNIYAAVYNEEHTKIFD
                     FSQRYGKQMVWIITTLIFAVALLYIDNRFYFFFAYIIYGFFILLLFAVLIFGKEVYST
                     RAWFEIGPVSIQPSEFAKAGTALAIARYLNSKGQELTRLSVLLPATGIILLPALLIAM
                     QPDMGTTVVYLSFFIVLFREGLSPYVFVTGILMIVLFFFALLFNQLYVVSGLIAAGFL
                     LSFTVARNFKTVLKGMFIFLLVGGIIYSVNHFLLKSLNINVILILSLIFSGIAFLWHI
                     YRYKAKGVLIIYLFVLGSVLYVNIVDYLYNNALRPHQKERIEILLRIKSDPRGSGYNI
                     NQSVISIGSGGFLGKGFLRGTQTKFRFVPAQSTDFIFCTVGEEWGFVGATVVIGLYVL
                     LLLRLLFLAERQRSAFSRIFGYGVISVLLIHFFVNIGMATGILPVIGIPLPFFSYGGS
                     SLWGFTLLLFIFLRLDVARHEYLI"
     gene            complement(8867..9478)
                     /locus_tag="PLG42_04000"
     CDS             complement(8867..9478)
                     /locus_tag="PLG42_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOU30408.1"
                     /translation="MNVLAHIYLSGDSDKIIIGNYIGDYVKGRDYLKYPELVRKGIIL
                     HRHIDAFTDRHPVVHRSKLFFSRKYYKYAGVIVDILYDHFLTKEWDFFSRKPLESVTY
                     NFYRALVNHYEILPDNVRQMMPFFIINNWIESYQTVKGIKRVLNALSKRSSLPNETRW
                     AIKTLKKNYFSLQDDFMEFFPQLIDYVEKDFGIPLSHDISIPF"
BASE COUNT         2888 a         1741 c         2148 g         2990 t
ORIGIN      
        1 attgtttttc agggttgtaa aagtcgcttt caaccagaga aggaaaattc tgagaaattc
       61 catcagggca tctttcaggc ttttatcaga agataacccg gtactttcgt tgcgtcccga
      121 acagctagcg gtgcagcagt ttgttgattt aacgggctgg attgcggaga atgcactttt
      181 agaatgagta acctattgcc tccacagggt tcatccgggc agctgtatat gcaggagcat
      241 accctgcaac cacacctatt gtacccgata taaatattgc gagcaggata tttttgacag
      301 tcaggaacat attaagctca tacaggtaat ttatgccgag agtcccaata aatacaaaca
      361 ggattccgag aaggcctccc agaacagaca ggattacaga ttcagtaagg aactgaagga
      421 gtataaagga tctttttgcc ccgagtgctt tctgtattcc tataatattt gttctttctt
      481 taactgatac gaacatgatg tttgctacac cgaatccgcc caccaggatg gcaaagccac
      541 ctataaacca tccgcctgca tttatacctg caaaaaccgc tttgaatcct tcagttagaa
      601 gacttgccct gtttaccgaa aaattgttta tctcccccgg cctgagtctt cgggcagctc
      661 tcagaatcat tactgcttca tcagagagct cttcgattgc aacacccggg cgtgccttaa
      721 tcataagctg tgtttcagca ttcctgcgac gaagattaat aaatgttttt ccaaaattca
      781 gaggtacaat tgttatctca tccatcccgc tgtcacttat tccacccttt ccttctttct
      841 taatgacacc tattacagtt gccctgtgct gcccgatagt aatctctttg cccacagggt
      901 tgctcttttc aaaaaggcgc tcggcaacct ctgcaccaat tattgcgaca ttcttacctg
      961 aagaggcttc gtataaagaa aaatacctgc ctttctcgat ttcaaaactt ctgatatcct
     1021 tgatttcata agtggctgca cccaccagag cctcacttat tgagttcttg ttgtatttaa
     1081 tcgttgcagg ctgaaaaacc acaaaacttg ccgactgact ttttgtagat tttctggtaa
     1141 tagcctcaaa atcttcaacc gatactgcag gccatcgaat aatgtcccac cagttgagat
     1201 ctggatcgaa cgaccatgga aatttttgaa catatattac attatcaccc aatgttgaga
     1261 tactgtctct tattgatttt tccatccaat caagaacagt gaaaacagaa attatacaga
     1321 atattccaat tgttatgcca aaaagagaga ggaaagttct gagtttgttc acataaatag
     1381 aactgaaggc gaaagatatg ccttcgtata tcagttgaag caaggttcct gcagcagttt
     1441 ttttgtgttt ttcctgcata agtttctgga aagacccaaa gataataaat tcatggtttt
     1501 ctatttctaa tcctgcggaa atatccagaa ttcagtcaga tatttcctta aaaagatggc
     1561 ttataattgg tttatgctct caaatgtttg ccatttcagg atgagaaaga cagaatatct
     1621 aaattattga gatattattg agtatattga aaatttatct atttttatcg ctcttagggg
     1681 acaagatttt caaaacataa atttcagata atgaacgaca aaaaaataaa aagcgcactg
     1741 gtttcagttt attacaagga gggtctggat gagattttga aaattcttaa tgaactggga
     1801 gtcaggatta tttcaaccgg tggcacctat agttacataa agggtcttgg tattgaggcc
     1861 gaaaccgttg aaagtttgac aacatatcct tcaattcttg gtggcagggt taagacactt
     1921 catcctgtag tttttggagg tattcttgct cgaaggggca atgccgaaga tgtcggtcag
     1981 atgaagagtt acaatattcc ggaaatagac cttgttattg ttgaccttta tccttttgag
     2041 gaaacagtag ccaggtcagc cgtggaagag gagataattg aaaagattga tattggaggg
     2101 gtttcgcttt tgagagcggc agccaagaat ttcaatgacg ttcttgtggt ttcttcaagg
     2161 tcgcagtacg gaaggctggc cagtcttctg aaagaaaaag gtggttatac cagtattgag
     2221 gaacgaaaac tttttgctgc tgaggcattc aaggtgactt cagggtatga tactgcaatt
     2281 ttcagatatt tcaacaggga ggctaattta actgctttca gggaaagttt tgattcattc
     2341 atgtctctcc ggtatgggga gaatcctcat cagaagggag tctttttcgg tgattttgaa
     2401 atgatgtttg ataaacttca tgggaaagaa atttcattca acaatttact cgatgttgat
     2461 tcggccattg gattgattga tgagtttgcc gaaacgacag ttgcaattat aaaacataac
     2521 aacgcctgcg gggtagccac aagagataac ctgatggatg cgtggaagga tgctctggcg
     2581 tgtgatcctg tatcagcttt cgggggaatt attgtaacta atcgtttagt tgatgaagtc
     2641 acagctacgg agattgacaa tctgtttttt gaaatattaa ttgctccggg atattctgac
     2701 agtgcactgg agattttgaa gcgtaaaaag aacaggatta ttcttcttag aaagagttct
     2761 gatgtttccc cgtttgttta cagaagtatt cttaatgggg ttctgtggca ggaaagggac
     2821 atgcacacag aggatgttgc tgatatgaaa cctgttacag agagggtgcc cgatagcagg
     2881 gaatatgagg atcttgtttt tgcaaacaag gttgtgaagc atacaaaatc gaacgctata
     2941 gtactggctc gtaacaagca gctatgtgcc agcggtacag gtcagacttc gagggttgat
     3001 gctctcaggc aggcagtgga aaaagcagga atgtttggtt ttgatctgaa aggtgctgtg
     3061 atggcttctg atgctttctt tcctttcccc gactctgtag aaattgctca caaggcaggt
     3121 ataacggctg ttgttcagcc tggtgggtcg gtaagggatc aggaatcaat agatttttgc
     3181 aataaacatg gaatggcaat ggtttttaca ggtataaggc attttaaaca ttgataataa
     3241 ggagttatgg gactattttc atttttgaca caggagatag ctatagacct cgggacggct
     3301 aatactgtaa ttattcacaa cgacaaggtg gttgttgatg aaccttcgat tgtggcgatt
     3361 gaccggaata ccggcaaact gatagctatt ggtgaacagg caaggcagat gcatggaaag
     3421 acacatgaaa atattgttac aataagaccg ctgagggatg gtgtcattgc cgatttcaat
     3481 gctgccgagc tgatgataag gggtatgatt aaaatgataa acaaaggttc aaaacttttt
     3541 acaccgtcgt taaggatgtt ggtgggcatt ccctcaggca gtacagaggt tgaaataaga
     3601 gctgtgcgcg attcatcgga acatgcgggg ggcagggatg tatatcttat tcatgaacca
     3661 atggctgcag ccataggtat aggactcgat gttgaagctc ccgaaggctc aatggtagtg
     3721 gacataggag gtggtacaac cgaaatagcc gtaatagccc ttggaggtat cgtatgcagt
     3781 aagtcgatcc ggattgcagg cgatagcttt acttccgaca tacaggcata tatgaggcat
     3841 cagcataaca ttaaggtagg agagaagaca gcagaagata tcaaaattgc agttggtgct
     3901 gcattgcccg atattgataa tccacctcca gattttatag tgagaggtcc aaatatcatg
     3961 accagtttgc ctattgaagt gccggtttca taccaggaga tagcttattg cctcgacaaa
     4021 tcactgtcaa aaatcgaggc tgcagtactg aatgtgctgg aacagacacc ccctgaactt
     4081 tatgcagata tcgtcaataa aggtattttc ctgactggag gtagctctct gctcagagga
     4141 ctcgataaga ggcttaccga caagataaac attaattttc atgtggtaga ggatcccctg
     4201 cgagcggtgg caagaggtac ggcagtagca cttaaaaaca ttgataaatt ccagttcctg
     4261 atgagataag atgaaaaggt aaatgagaaa tctgctactt tttattgtaa agtacaacag
     4321 aataattctg tttatcctac ttgaaatgat agcagtttat ctcatcagta attcatatag
     4381 atatcataat tctcagttaa tcaaaggtgt acgaaaacta acccttgcat gggaaggggt
     4441 aattactgac acaaaaaact atcttagaat caaaaagata aatgacgatc tgcaggctga
     4501 aaacactgca ctttggaatc tgatagaaaa aaatataata aaaaccgact catccttttt
     4561 acagataaat gatactgctt caggagccca atatgaagta cttcccgcaa gtgtggttaa
     4621 taattctgtc aacagaataa agaattattt tactattgac aggggtaagg atgacggcgt
     4681 tgacgtaaat atgggtgtaa taagtccatg gggtgtggct ggcatcaccg tggcatcatc
     4741 aaacaattat tccattgtaa tgccagtgat aaatacggat ttcagactca gcagcaaaat
     4801 aaagtcgaat gactattttg gctcactcat atgggatggg aaagacaaca ggtatgccgt
     4861 tttaaatgat ataccacagc atgttgcatt aaatgaaggt gatactattg taaccacagg
     4921 ttactcggca atattcccac caggaattat ggttgggacg ataagtgaat tcaagaaatc
     4981 gggcagcgat ttttattcga taaaagttcg tattgccact gacttcagaa agctcactta
     5041 tgtgaaagta ataagaaata aaaggcagga ggaacagtta aatcttgaaa agggatttca
     5101 atgattaatc agctgttaag atatactatt atatatatag tgctgctact gttgcaggtt
     5161 tttgtattta acaacataca gtttagcgga tatgtaaatc catatattta cattctattt
     5221 gttctgctgc tgccttatga aatatcaggt tggcttttgc tgattatatc attttttgca
     5281 ggatttttta tagatatttt tatgggtaca gctggcatgc atgccggggc taccgttgct
     5341 gcaggttttg ccaggcccta tattctgagg gctatttcac cccgtgacgg atatgtggcg
     5401 gatagagaac tttctatggt agcttacgga tttaactggt ttttctttta tgccctttta
     5461 gttgttgctg ttcaccatct ggttcttttt tacctcgaaa tattcagctt tgaaggtttt
     5521 ttcaggacat tcttaaaagt aattttcagc actgttttta cagttatatt tatcattttt
     5581 gcagagtata taaggaaagg ccgatagagg tttgtaatga aggatcttta tgtcaacaga
     5641 agaataatag ttgcagctct gattattatc ccggctgtta tacttcttgc gaggctcttt
     5701 cagattcagg tggttgaaac ttcataccgc atatcagccg aaaacaatgt tttacgttat
     5761 gttactcaat atcctgcacg cggactgata tacgaccgca acagaaaact gctggtattt
     5821 aaccagccgg cgtatgatct gatggtaatt cccggacagg tttcaaagat tgacacgggg
     5881 gaattctgca aatcactgaa tatcaccagg gagtatttca gggagaggat gaaggcagca
     5941 gcaagttatt caaaaagggt tccttcaata tttatgaagc agatttcttc cgaaatatat
     6001 gccggctttc aggaaaaatt gttcatgttc cccggtttct atgtacagac aagaacactc
     6061 agaaaatatt caaaacctat tgctgcacat gtattgggtt atgtaagtga ggttgacgaa
     6121 aatgttatta agaataatcc ttattacaag ccgggtgatt acattgggaa aacgggcatt
     6181 gaagaagcat acgaagctga attgagggga aagaaagggg taaagatatt tttggtggat
     6241 gtttacggaa gggttaaagg ttcgtatgag gaggggaggc tcgacactgc agcagttcag
     6301 ggaaaggata ttgtatgtac cattgattca gatcttcagg aatatgctga aactctgatg
     6361 aaaaacaagt cggggagtat tgttgccatt gaaccagcaa caggggagat tttgaccctg
     6421 gtttcttctc ccacttatga tccttcactg cttgttggta gggtcagatc agaaaacttt
     6481 gcaaagctgc aggctgatac aaccatgccg atattcaaca gggcaattat ggcctcatat
     6541 cctccgggat caacatttaa aacagtcaac ggactcatcg cccttcagga gaaagtcatt
     6601 tctccttcga caattttcgg atgcaatcca ggttatcttt ttgtaggatg ccatatacac
     6661 gaatcaccgc ttgaccttgt gggagcaata aagaactcgt gcaatgccta tttctgccag
     6721 gcattcagaa gaattctaga aaatcctgcc tataaatcgg ttagtgatgc atatgagcaa
     6781 tggagaaatt atttattgca gtttggaata ggcagaagtc tgggctcaga ccttgtaaat
     6841 gaaaaatcgg ggattcttcc tacagtgtcg ttttatgatt cccgttatgg taaaggccgc
     6901 tggaaagcac tcactgtaat atctctggcg attgggcagg gagagatagg tgtttctcct
     6961 ctacagatgg ctaatatgac agcagcgata gcaaacaggg gattctatta caaccctcat
     7021 attgttaaag caataggtga ttctgtgaat gtcaccagag gactggcgga aaaacataca
     7081 attgaaattg attcatcgaa ttttgaatgg ataataaagg gaatggaaga ggctgttaat
     7141 ggaggtaccg gtgctactgc aagaattgca gcaatagatg gtataactgt ttgcggtaag
     7201 acggggacag cacagaatcc tcatggtaaa gaccattcta tatttattgc atttgctcca
     7261 aaagaaaaac cacagattgc catagcagta tatgtcgaaa atgcaggttt tggtgctact
     7321 tatgctgctc ctgttgcaag acttttaatc gaaaaatacc tgaagggaac aatcactaac
     7381 aagtcgctcg aagattatat tctcaaccta aatctgatga atggatggag agaagagtga
     7441 atatatggag ggctgttgac aagattacca ttgtaatatt tttgcttctt gtaatactgg
     7501 gctggcttaa tatatatgct gcggtctata atgaagaaca cacaaagata ttcgattttt
     7561 cccagcgtta tggaaagcag atggtatgga ttattacaac actgatattt gctgttgcac
     7621 ttctttatat cgacaatcgt ttttatttct tttttgccta cataatttac gggttcttca
     7681 ttttacttct ttttgcagta ctaatttttg ggaaagaagt atatagcacg agagcgtggt
     7741 ttgaaatagg acctgtgagt attcagccct cggaatttgc aaaagcaggt acggcgcttg
     7801 ctattgcccg gtacctgaat tcaaaaggac aggaactgac acggcttagt gtactgctgc
     7861 ctgcaacagg tatcattctg cttcctgcat tgctgatagc catgcagccc gatatgggaa
     7921 ccactgttgt ttatctgagt ttctttattg tcctcttcag ggaaggcctg agcccttacg
     7981 tgtttgtaac aggcattctc atgattgttc tattcttttt tgccctgttg tttaaccagt
     8041 tatatgttgt ttcaggtctg atcgctgcgg gctttcttct ttcctttacc gtggcaagga
     8101 acttcaaaac agttcttaaa gggatgttta ttttcctgct cgtcggaggt attatttatt
     8161 cagtaaatca ttttctgtta aagtcattaa atatcaatgt aatattgatt cttagcctca
     8221 tattttcagg gattgccttt ttatggcaca tatacaggta caaggctaaa ggagtactta
     8281 ttatatacct gtttgtactt ggcagtgtat tgtacgtaaa tattgttgac tatctgtata
     8341 ataatgcatt aaggccacat cagaaggaga ggattgaaat acttttgcgc ataaaatcag
     8401 atccgcgcgg atcgggttat aatataaatc agtcggtcat ttcaattggt tctggaggtt
     8461 tcctggggaa aggcttttta aggggaaccc agacaaaatt cagattcgtt ccagcacaga
     8521 gtacagattt tattttctgc acagtgggtg aggagtgggg ttttgttggt gccacagtag
     8581 taattggcct ttatgttttg ctattgctgc gtttgctttt tcttgcagag cggcagcgct
     8641 cagctttctc ccgtattttc ggttacggtg tgatatcagt actgctgata catttctttg
     8701 taaatatagg aatggctacc ggaattttgc cagtcattgg cattccgctt ccttttttca
     8761 gctatggtgg cagctctctc tggggtttta ccctgcttct gtttatcttc ctcaggctcg
     8821 atgtcgcaag gcacgaatat ctaatttgaa gagaaatatg tactcttcag aaagggatgc
     8881 tgatatcgtg agatagcgga attccgaaat ctttctccac ataatcaatc agctgtggaa
     8941 aaaattccat aaagtcatcc tgaagtgaga aataattttt cttaagtgtt ttaattgccc
     9001 accgtgtttc gttagggagg gatgatcttt tgctcagagc gtttagtacg cgcttgatac
     9061 ctttgacagt ctggtatgat tcaatccagt tgtttataat aaaaaacggc atcatctgcc
     9121 ttacattatc aggcaatatc tcataatgat taacaagtgc acggtaaaag ttgtaagtaa
     9181 cactttcaag aggttttctc gaaaagaaat cccattcttt tgtcagaaaa tgatcatata
     9241 aaatatcaac aataacacca gcatatttgt aatactttct cgaaaaaaag agtttgcttc
     9301 ggtgaacaac aggatgcctg tcggtgaatg cgtcaatgtg cctgtgaagt atgatccctt
     9361 tacgtacaag ctcaggatat ttaagataat ctctcccttt tacatagtcg cctatgtaat
     9421 tcccgatgat tatcttgtct gaatcacccg acagatatat gtgagccagt acgttcatta
     9481 tttatattca atttcaatac tacaaagaat ttgtcataaa gttattcaac cttacaaatt
     9541 ttttaggtgt ttagaagtga taacgtatta ttaattcaga agattacggg aaagtgaaaa
     9601 atatttttaa tggtagaaat aaattaaatg ctgttaattg attttaatca gggatttttt
     9661 aaaaaagtta gagtaatctt gggggaaata gtaaatttgg tgcattaaaa aattttataa
     9721 tagaagatta atatggctgt aaagaatgtc atcattatag gagcagc
//
LOCUS       DAOSNB010000060         9700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7005_length_9700_cov_5.064075, whole genome shotgun sequence.
ACCESSION   DAOSNB010000060 DAOSNB010000000
VERSION     DAOSNB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_9700_cov_5.064075"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLG42_04005"
     CDS             complement(<1..243)
                     /locus_tag="PLG42_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30409.1"
                     /translation="MKNLSTICLTIVLIFIILPKTFSQQFNIPTELFSRAERTWYQET
                     SLNADVKAFCEAVGRLSNYAHTEVIGRTKEGNDLIIV"
     gene            complement(274..3228)
                     /locus_tag="PLG42_04010"
     CDS             complement(274..3228)
                     /locus_tag="PLG42_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOU30410.1"
                     /translation="MSKIIIKLFTSGIFFLNLIIITAQNSEPPFLKYLNHPWVDSVLK
                     SLTPEEKIAQLIWISAYSNRGIDYDAEISNTVKSFSPGGFVFFQGTPRKQIEMINYFR
                     KISKVPPIVAIDAEWGPGMRLEGIERFPYQMTLGAIQNDSLIYLMGAQIARMLKRTGV
                     DVNLAPVADVNNNPANPVINVRSFGEDPENVSRKALMYMKGLQDNGVIAVGKHFPGHG
                     DTEVDSHVDLPVIKHDRDRLNSIELVPFKNLIANGITGIMPGHLSVPVLDPTENMPVT
                     LSKSILTDLLKTELGFKGVAISDAMNMGGIVKYSSPGEVETQSLKAGMDVLEFVLDPG
                     QSIKSILDNLKSGKISIAEIDEKCRKVLAMKYWAELYKFETLKTENLMTDLTDPSISA
                     LIRELYANALTVLNNEQNIIPVKNLDRLRIATLAAGKSEITDFQKRVSSYTKADHFFI
                     NSFDDPKVNELIENLASYDIVIAGFYGPDRAPSNYTAASDFNSLAEKLNNRTRCIIVW
                     FGNPYLIARMESLTNSAGLVLAYQNNVITEDLSAQLIFGGIGASGKLPVTINEKYPCG
                     YGLITPGNLRLKYGLPENAGVSSVKLRQKIDSIANAGIQAKAYPGCEVMIARKGTVIF
                     HKCYGYHTYENRTPVDENDMFDLASVTKVSAATPALMLLQSAGMFNPDEKLGKYFTDF
                     RRSNKSDMVIRDMLAHQAGLVAWIPFWKSTVKKDGSFKPRTFSYGYSEKYPLKVAQGL
                     YIHKNYKDKIFKEIKKSPVSNEKKYLYSDLTFIIMPEVIERLSGTTWYKLVEDSIYHK
                     IGAFNICFNPYLKYPPERIVPTEYDSLFRKQQLHGTVHDEGAAMLGGISGHAGLFATA
                     NDLMKLMELYRNMGRYGGEQLISEEVMKEYTRVQFPENKNRRGLGFDKPLLENDTLPP
                     EKTYPTYGASPESFGHSGYTGTFVWVDPVHEVSYVFLSNRVYPARNNNLLSDMNIRTN
                     ILQAVYDSIINP"
     gene            complement(3282..4118)
                     /gene="murQ"
                     /locus_tag="PLG42_04015"
     CDS             complement(3282..4118)
                     /gene="murQ"
                     /locus_tag="PLG42_04015"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOU30411.1"
                     /translation="MSITEMPSNFDNLEKMSVHELLININKEDSKVPRAVRKAIPQIE
                     ALVNRIVEKMAKGGRIFYLGAGTSGRLGVLDASEIPPTFGVHDRIIGLIAGGETALRR
                     AVEAAEDDPEKAWKELQIFEINKNDTVIGIAASGSTPYVIGGLTRARENGILTGCITC
                     NPKGKIIEVVDYPIVVVTGPEFVTGSTRMKAGTAQKLVLNMISTTVMIKLGRVKGNKM
                     VNMQLTNKKLVERGIRMLVEELNIQREAARLLLFQHGSVRKALEYYREKNDGNKGKNY
                     DI"
     gene            complement(4147..4998)
                     /locus_tag="PLG42_04020"
     CDS             complement(4147..4998)
                     /locus_tag="PLG42_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOU30412.1"
                     /translation="MILIADSGSTKTEWKVIEDGVPGDTYLSSGINPYFLGVEKIVNL
                     LKTEIPLLENREFNSVWFYGSGCNTEVKKDVIRDALSRFVRAGKIFVESDLLGAARAL
                     CQNEPGIACIIGTGSNSCYYDGKEIVSNVAPLGYILGDEGGAAVLGRKLLSAVLKKQV
                     SQEVIDRFFKTYEITPAQILENVYSMPFPNRFLGGFASFISSNIDMPELQEIVRTSFD
                     EFIKRNILQYPQSKELPVHFTGSIAWNFKIFLEELLVKNSLKPGKFLLHPMPELVKFH
                     IKNIDKL"
     gene            5245..7569
                     /locus_tag="PLG42_04025"
     CDS             5245..7569
                     /locus_tag="PLG42_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30413.1"
                     /translation="MDMKSFTGVEKSFFSRSGRTGFTTYLPYIVVPNIPSLGLLTSLR
                     FLEWVSENPEGVVSLPTGKTPEYFIKWTIKLLNEWDKKETRKIMDENGFILKKKPQIN
                     GIRFVQIDEFYPIDPRQTNSFYNYVINFYIKGFGLDPERALLINSEEIPLAEGKHYTE
                     VFPDNLIDLTLRHREPLTPLEKIQQESIFRIDNWCMEYENKVREMGGIGFFLGGIGPD
                     GHIGFNIRGSDHNSTTRLTATNFETQAAAAGDLGGIEISRNRLVITIGLGTITSNPEG
                     VSIIFAAGESKAGVVRDALENKPDNLYPATVLQRQKNARFYLTEGAAVQLNDSIEKYY
                     REGEWTFEKTERAIYDLCLKINKYAHNLTLEDLKNDRYCSMIPGVSLDTVREVIESTK
                     KKIEAGGSNEYNQVFLHTGPHHDDIMLGIFPCIIRQLREKSNTFYFAIMTSGFTSVTN
                     SLIKELLLATLDHIKKGKIQMLNYKDFFEAGYKIKFDKDVAHYLDKVADNDEEGKLRG
                     VCHRITRALVDIYRVKSIEELTDTIEKVVKVLDSSYSGEKNPPDIQKLKGMLREFEEE
                     LVWAHYGVKVQNIEHLRLGFYTGDIFTPLPERKRDIEPILNLLKRVKPTVLSLVLDPE
                     GSGPDTHYKVLQAIAEALRQWKSEEDISSLRIIGYRNVWYKFHPAEANVFTPVSLNSM
                     AVLDWSFRNCYISQVKASFPSPEHDGPFNQLSQKIWVEQFKRVQLILGKDFFYDNESP
                     KIRATHGMIYYKEMKVEEFLKYARELEKSIEGEF"
     gene            7649..9058
                     /locus_tag="PLG42_04030"
     CDS             7649..9058
                     /locus_tag="PLG42_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOU30414.1"
                     /translation="MIKDELLRLLFRDFTGKSPESLTVLPKSGSDRIYCRMVRGDFSV
                     LGVWNPSRKENDAFTGFAESFIRAGLRVPRVYQYLPEEMVYLTEDLGDLDLCMWVEEK
                     RKKPEGENEIVEMYKSVLKELIRFQFDADREIDYNLCYPRRKFDRNSVMWDLNYFKYM
                     FLKLSGTPFNEQLLEEDFVKLADEIEQVWSDGFLYRDFQSRNIMIKEGIPWFIDFQGG
                     RKGAPHYDLASLLLDPYVELGREMHDELLGFYFSLLNQRMNTEKDDFMNEYRMFGVVR
                     LLQALGAYGFRGLYERKPNFTSSIPAAVRQLEKLFEGEGVRKKFPELLRIVENLYTRW
                     ASTDTDHRPKPEVIIESFSFEKGIPETFTSDGGFMFDCRHIVTTENTEVANNTGNNKT
                     LADYLDDRKDVQDLTENAYRIIAGALAAEGIGKVKTLRVCFGCSSGKQISVFCAEKVA
                     EMLRREKLAEVIVFHHQIR"
     gene            9111..>9700
                     /locus_tag="PLG42_04035"
     CDS             9111..>9700
                     /locus_tag="PLG42_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU30415.1"
                     /translation="MKAMIFAAGKGTRLGDITAARPKALLEIKGKSALRIAVEKLTLN
                     GFDDIIINVHHHANQMIKAIDNLKQEGFRLTVSDESEQLLETGGGLYKARWFFDDRPF
                     LLYNVDIITDIDLNEIYSYHIEKGGIATLAVRAYPDTRVFLTDKEGIIRGWRNKSTGE
                     EILTGNYGSLDEKAFSGIHVVEPEVFSYMKEGFYSMT"
BASE COUNT         2801 a         1851 c         2096 g         2952 t
ORIGIN      
        1 aacaattatc aggtcattgc cttctttggt cctgccgata acttctgtat gggcatagtt
       61 gctcagtcgg ccgacagctt cacaaaaagc ttttacgtca gcgttgagag aagtttcctg
      121 ataccatgta cgttcggctc ttgaaaagag ctccgtggga atgttaaact gctgtgaaaa
      181 agtttttggt aatataatga aaatcaatac aattgttaag caaatggttg aaaggttttt
      241 cattttttgt gatttttaaa tatttcgtag aggttatgga ttaattatcg aatcataaac
      301 cgcctgtaaa atgttcgtgc ggatgttcat gtcgctcagg aggttgttat ttctggcggg
      361 atataccctg ttggaaagaa acacgtagct gacctcgtga acagggtcaa cccaaacaaa
      421 cgtacctgta taacctgagt gtccgaaact ttcaggtgat gcgccgtagg tgggatatgt
      481 cttttcaggt ggtaatgtgt cattttcaag cagaggttta tcgaagccaa gccctctccg
      541 gttcttgttc tcaggaaact gaacacgggt atattctttc ataacctctt cgcttatgag
      601 ttgttcacca ccatatcttc ccatgttgcg gtatagctcc atcagtttca taaggtcatt
      661 ggctgtagcg aaaagcccgg catggcctga tatgccacca agcatggcag caccctcatc
      721 atgaactgtt ccatgaagct gctgtttcct gaaaagagaa tcatattcag ttgggacaat
      781 cctttcggga ggatatttaa gataaggatt gaaacatata ttaaaggctc ctattttatg
      841 ataaatactg tcttccacca gtttatacca tgtagtgcca ctaagccttt caattacttc
      901 aggcataatg ataaaggtaa gatcggaata gagatatttt ttctcgttgc taaccggact
      961 cttttttatc tctttaaaaa tcttgtcctt atagtttttg tgaatataga gtccctgcgc
     1021 tactttcagg ggatattttt cggagtatcc gtagctgaac gtgcgaggtt tgaaacttcc
     1081 gtctttcttt actgttgatt tccagaatgg tatccatgca accaatccgg cctgatgtgc
     1141 gagcatgtct cttataacca tatctgattt gttcgacctt ctgaagtctg taaaatattt
     1201 tcctagtttt tcgtcgggat tgaacatgcc tgccgactga agcagcatca gggctggagt
     1261 agcggccgaa actttggtta cggatgcaag gtcgaacatg tcgttttcat caacgggtgt
     1321 tctgttttcg tatgtatgat atccgtaaca tttatgaaag attacagttc cttttcgtgc
     1381 aatcataact tcacaaccgg gatacgcttt agcctgaata cctgcattag caattgaatc
     1441 aatcttttgc ctgagtttga cagatgaaac tccagcattt tcgggcaagc catatttcaa
     1501 acgcaggtta cccggtgtaa tgagaccata tccacagggg tatttttcat tgatggtaac
     1561 tggtagtttc ccacttgctc cgataccacc gaatataagc tgtgccgaaa ggtcttctgt
     1621 aatgacatta ttctgatagg caagaaccag cccggcagaa ttcgttaatg attccatcct
     1681 tgcaataagg tatggatttc cgaaccatac aataatacaa cgggtacggt tgttgagttt
     1741 ctctgcaagg ctgttgaaat ctgatgctgc agtataattt gaaggggctc tgtcggggcc
     1801 gtaaaaacct gcaattacaa tgtcgtatga tgcgagattt tcaattaatt cattaacctt
     1861 cggatcatcg aatgagttga tgaaaaaatg gtcagccttt gtatatgatg agactctttt
     1921 ctgaaaatct gtaatttcac ttttacctgc agcaagagtg gcaattctta atctgtcgag
     1981 attttttaca ggaattatgt tttgctcatt gttaagaaca gtaagtgcat ttgcatagag
     2041 ttctctgata agagccgata tggaagggtc ggtgaggtcg gtcatgagat tttccgtttt
     2101 cagtgtttcg aatttatata gttctgccca gtacttcata gcaagaactt tgcggcattt
     2161 ttcatcaatt tcggcaatgg atatcttacc tgatttcaaa ttatccagaa ttgatttaat
     2221 cgactgacca gggtccagaa caaattccag aacgtccata cctgctttga gagattgtgt
     2281 ttcaacttca ccaggcgatg aatatttaac tattcctccc atattcattg catctgatat
     2341 agctacaccc ttaaaaccca attctgtttt aagcagatcc gtgaggattg attttgaaag
     2401 tgtaacaggc atgttttcag tcggatcgag taccgggact gacagatgac ctggcattat
     2461 tccagtgatt ccgtttgcga tcaggttttt aaaaggaaca agttcaattg aattgagtct
     2521 gtctctatca tgttttatta ctggcagatc aacgtgtgag tcaacttcag tatctccatg
     2581 tccgggaaaa tgttttccga cagcaattac tccgttatcc tgcaagcctt tcatatacat
     2641 taatgcttta cggcttacat tttcaggatc ttcgccgaac gatctcacat taattacagg
     2701 gttggccgga ttgttattca catcagctac aggggcaaga ttcacatcaa caccggttct
     2761 tttgagcatc ctggcaatct gtgctcccat taaatagatt aaagagtcgt tctggatagc
     2821 acctagcgtc atctgatatg ggaatctttc gattccctcc agccgcattc cgggccccca
     2881 ttcggcatcg atggctacaa ttggtggaac ctttgatatt tttctaaagt aattgatcat
     2941 ttcgatttgt ttccgtggcg ttccctgaaa aaatacaaag ccgccgggag aaaaactctt
     3001 aactgtattg cttatttcag catcataatc tattccccgg ttagaatatg cagagatcca
     3061 tatcagctgg gcaattttct cttctggtgt aagggattta agaactgaat ccacccatgg
     3121 gtggttgagg tacttcagaa aaggtggttc agaattctgg gcagttataa tgatcaagtt
     3181 caaaaagaaa ataccagaag tgaagagttt gattattatt ttgctcatac agtacttcgt
     3241 ttttttgaag attactttac tcgattctta ttgaggttaa tttatatgtc atagtttttc
     3301 cccttgttac catcgttctt ttccctgtaa tattcgagag cttttctcac tgaaccatgc
     3361 tgaaaaagaa gcagtcgtgc agcttctctc tggatgttaa gttcttcaac gagcattctt
     3421 attccgcgtt caactaattt cttgtttgtc agttgcatgt tcaccatttt gttgcctttg
     3481 acccgtccga gttttatcat taccgtggta cttatcatgt tgagtaccag tttctgggca
     3541 gttccggctt tcattctggt gctaccggtt acaaattctg ggcccgtgac aaccacaatg
     3601 ggataatcta caacttctat aattttccct ttcgggttgc atgttatgca tccggtaaga
     3661 attccgtttt cgcgggctct ggtcagaccg ccaattacat agggtgttga ccctgaggca
     3721 gcaatcccga taactgtatc gtttttgttt atttcgaata tctgcagttc tttccatgcc
     3781 ttctcgggat catcttcggc ggcttctaca gcgcgcctga gggcagtttc accgccggct
     3841 ataaggccta ttatccggtc gtgaacacca aatgttggag gtatttccga agcatcgagc
     3901 actcccagcc tgccactggt tccagctccc agatagaata ttcttcctcc ctttgccatc
     3961 ttttctacaa tcctgttaac cagtgcttca atctgaggta ttgcctttcg tacagcccgg
     4021 ggaacctttg agtcttcttt gtttatgtta atcagcaatt catgaactga catcttttca
     4081 agattgtcaa aatttgaagg catttcggtg atactcaaat gctctttctg ctttcttgcc
     4141 acaatattat aatttatcta tgtttttaat gtgaaattta accagttcgg gcattgggtg
     4201 aagtagaaat tttcctggct tcaggctgtt tttgaccagc agctcttcga gaaagatttt
     4261 gaaattccat gctatgcttc cagtgaaatg aacaggaagt tctttcgatt gggggtattg
     4321 aaggatgttt ctttttatga attcatcaaa cgatgttcgc acaatctcct gcagttctgg
     4381 catatctatg tttgaggaga taaaacttgc aaaacctccg agaaagcggt tagggaaggg
     4441 catggaataa acattttcaa gtatctgagc tggtgttatt tcataggttt tgaaaaatct
     4501 gtcgatgact tcctgtgaga cctgtttttt aagaacagcc gataataact tcctaccaag
     4561 tacagctgct cctccttcat caccaagaat ataaccgagg ggtgctacat tcgatactat
     4621 ttcttttcca tcgtaataac atgaattaga gccagttcca attatacagg caatccctgg
     4681 ttcgttctgg cataaagctc gagcagcacc gagaagatcg ctttcaacaa atatttttcc
     4741 agcccttaca aatctgctga gagcatccct gatgacatct tttttaacct cagtattgca
     4801 cccgctgccg taaaaccaga cactattgaa ttccctgttt tcaagaagag gaatttccgt
     4861 tttaagcaga ttaactattt tttcaactcc aagaaaataa ggatttatcc ccgaagataa
     4921 ataggtatct cctggaacac cgtcttcaat taccttccat tcagtcttgg tcgagccgct
     4981 gtcggcaatc aatatcattt atttctgaaa tgccctgcta atttaattta ttttggatga
     5041 ttttgcagag aattatctat tatcttttgt tttagattga gctattaagt ggaagtggtt
     5101 ttatatattt gatggcagat ttattgaaaa aaattatttt ttctcatatt tttttattta
     5161 tttgattgaa aataatttga agactttttt atcggctttt gtaaaaacaa ttgatagtga
     5221 gtcagaacat taaatatgca ttttatggat atgaaatctt ttacaggtgt tgaaaagagc
     5281 ttttttagcc gttcaggaag aaccggattt acaacttatc ttccttatat agtggttccg
     5341 aatataccat cacttggatt attgacctcg ctgaggtttc tggaatgggt ttcggaaaat
     5401 ccggaagggg tggttagtct tcctaccggt aagacgcctg aatattttat taaatggaca
     5461 attaaacttc tgaatgaatg ggacaaaaag gagacgagaa agataatgga tgagaacggc
     5521 tttatattga agaagaagcc tcaaataaac ggcatccgct ttgttcagat agatgagttt
     5581 tatccgatag atccacggca gaccaacagt ttttacaact atgtcattaa tttttacatt
     5641 aaaggctttg gtcttgatcc tgaaagggca cttcttatta attctgaaga gatccctctt
     5701 gccgaaggaa aacattatac tgaggttttc cctgataatc tgattgatct tactctgagg
     5761 cacagggaac cgcttactcc tcttgagaaa attcagcagg agtctatttt cagaattgac
     5821 aactggtgca tggagtatga aaacaaagtc agggagatgg gaggcatcgg gtttttcctt
     5881 ggaggtattg gacccgatgg tcacataggt tttaatatca gggggagcga ccataattca
     5941 acaacccggc taaccgcaac aaattttgag acacaggcgg ctgctgcagg ggacctggga
     6001 gggatagaaa tatcaagaaa cagactcgtt attactattg ggctgggaac cattacttcc
     6061 aaccccgaag gtgtatcaat aatctttgca gcaggtgaat ccaaggcagg ggtggtaagg
     6121 gatgccctgg aaaacaaacc cgataacctt tatccggcta cagtattgca gcggcagaaa
     6181 aatgccaggt tctatctaac tgagggtgcc gctgttcagc ttaacgatag tattgaaaaa
     6241 tattaccggg agggagaatg gactttcgag aaaactgaaa gggcaatcta cgacctgtgc
     6301 ttgaaaataa acaagtatgc ccataacctt actcttgagg atcttaaaaa cgacagatac
     6361 tgttcaatga ttcccggggt aagtctcgat acagtgcgtg aagttattga gtcaacaaaa
     6421 aagaaaatag aagctggtgg aagtaacgaa tataatcagg ttttcctgca taccggacca
     6481 catcatgatg atattatgct gggcattttt ccctgcatca taagacagtt aagggaaaaa
     6541 tcaaatacat tctattttgc tataatgacc tcaggcttta cttctgtgac aaactcactc
     6601 attaaagaat tgcttctggc taccctcgat catataaaaa agggtaagat acagatgctg
     6661 aattacaaag atttttttga ggcaggctat aagattaaat tcgacaagga tgtggctcat
     6721 taccttgata aggttgctga taatgatgag gaaggaaagc tgagaggtgt ctgccatcga
     6781 attacaaggg cactggttga tatttacagg gtgaagtcaa tagaggaatt gactgacaca
     6841 attgagaagg tggtcaaggt actcgattct agctacagtg gtgagaagaa tcctcccgac
     6901 attcagaaac ttaaaggtat gctccgggaa tttgaagagg aattggtgtg ggctcattat
     6961 ggagtgaaag ttcagaacat tgaacatctc cgactgggct tttataccgg cgacatattt
     7021 acaccattac cagagcgcaa gcgtgatatt gaaccaatac tcaatttact aaagcgcgta
     7081 aaacctactg tactcagcct ggtactcgat ccagaaggct caggtcctga tactcattac
     7141 aaagtacttc aggcaatagc tgaagccctg agacaatgga aaagcgagga agacatatct
     7201 tcactcagga taataggtta caggaatgta tggtataaat tccatccggc tgaggcaaat
     7261 gtgtttactc ccgtctctct taactcaatg gcagtactcg actggtcgtt cagaaactgc
     7321 tatatcagcc aggtaaaagc ttcattccct agccctgaac atgacggtcc tttcaaccag
     7381 ctctcacaga aaatatgggt agagcagttt aaaagagtac agctaatact cggaaaggat
     7441 ttcttctacg ataatgaaag ccctaaaata agagcaacac acggaatgat ttattataaa
     7501 gaaatgaaag ttgaagagtt cctgaagtac gcacgcgaac tcgaaaagag tatagaaggc
     7561 gaattttaat caatttctgg ctcaggaatc agttaaaagt ttgcttttat ttagttatca
     7621 ataattaatt gaagtcatac ccggtcagat gataaaggat gaattgctga ggttgctttt
     7681 cagggatttc acgggtaaga gtcctgaatc tcttactgtt ttgccgaaat caggctccga
     7741 cagaatttac tgccgtatgg taaggggaga cttttcggtt ctgggcgtat ggaacccgag
     7801 ccgaaaagaa aatgatgcat ttacaggctt tgcagaaagt tttataaggg caggactgag
     7861 agttcccaga gtatatcagt atcttcctga agagatggtt tatctcactg aagacctcgg
     7921 tgacctcgac ctttgcatgt gggtggagga aaagagaaag aaacctgaag gggagaacga
     7981 aatagttgag atgtataaat ctgttcttaa agagcttatc agatttcaat tcgatgccga
     8041 cagggagatt gattataatc tttgctatcc aaggagaaaa tttgacagaa attcggtaat
     8101 gtgggacctg aattacttca agtacatgtt tttgaaactt tcagggactc catttaacga
     8161 gcaacttctg gaagaagatt tcgtaaaact ggcagacgaa attgagcagg tatggtccga
     8221 tggttttctt taccgcgatt ttcagtcgag gaacataatg ataaaagaag ggattccatg
     8281 gtttattgac ttccagggag gcagaaaagg agcgccacat tatgatttgg catctcttct
     8341 tcttgatcct tatgtggaac tgggcaggga aatgcatgat gaactccttg gcttttactt
     8401 cagcctcctg aatcagagaa tgaatactga gaaggatgac ttcatgaatg aatacaggat
     8461 gttcggagtt gttagacttc ttcaggcact cggagcctat ggattcagag gcctttatga
     8521 acgaaagccc aatttcactt ccagtatccc tgccgctgta aggcagctag aaaaactgtt
     8581 tgaaggtgaa ggagtacgaa aaaaatttcc tgaattattg cgcattgttg aaaaccttta
     8641 cacacggtgg gctagtaccg acactgacca ccggccgaaa cctgaagtta ttattgagag
     8701 ttttagtttt gaaaagggta ttccggaaac ttttaccagc gacggaggat ttatgttcga
     8761 ttgcaggcat attgtgacta ctgaaaatac tgaagtagcc aataatacag gcaataataa
     8821 aaccctggca gattatctcg atgacaggaa agatgtacag gatctgacgg aaaatgcata
     8881 cagaataatt gccggagctc ttgctgcaga aggaatcgga aaagttaaaa ccctgagggt
     8941 gtgctttgga tgttcatcgg gaaagcagat ttcggtattt tgcgctgaaa aagtggctga
     9001 aatgctgagg cgcgaaaaac ttgccgaagt tatagttttt caccatcaaa taaggtaagg
     9061 ctgtcggtag atgtttaagc agaaataatt aacggcaggg aaggagcggc atgaaagcaa
     9121 tgatatttgc tgcaggaaaa ggaacaaggc tgggagatat tactgctgcc agacccaagg
     9181 ctctgctcga aattaaagga aaatcagctc tcagaattgc tgtggaaaaa ctaaccttaa
     9241 atggttttga tgacatcatt attaatgtac atcatcatgc caaccagatg attaaagcta
     9301 ttgataacct gaagcaagag ggtttcagac taacagtttc agatgaaagt gagcaactgc
     9361 ttgaaacagg aggcggatta tataaagcca gatggttttt tgatgacagg ccttttttgc
     9421 tttacaatgt ggatattatc accgatattg atttgaacga aatttatagt tatcatattg
     9481 agaaaggagg aattgctacc cttgcagtca gggcttatcc agatacccgt gtttttctta
     9541 ctgataaaga aggaataata agaggatgga ggaataaatc aacaggggag gaaatattaa
     9601 ccggtaatta cggttctctc gatgaaaaag ctttctctgg tattcatgta gttgaaccgg
     9661 aagtcttcag ctatatgaaa gaaggatttt attcaatgac
//
LOCUS       DAOSNB010000061         9629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7059_length_9629_cov_3.812409, whole genome shotgun sequence.
ACCESSION   DAOSNB010000061 DAOSNB010000000
VERSION     DAOSNB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7059_length_9629_cov_3.812409"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /gene="araA"
                     /locus_tag="PLG42_04040"
     CDS             <1..1487
                     /gene="araA"
                     /locus_tag="PLG42_04040"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOU30416.1"
                     /translation="LRKFEAWFVTGSQHLYGPETLKQVATDSHQIAAELDKNPAIPVK
                     IIFKPVLTTPDAIFELCSEANSAPNCIGLITWMHTFSPAKMWIRGLSALNKPFLHLHT
                     QLNRDIPWKTIDMDFMNLNQSAHGDREFGFITTRMRLNRKVVVGHYKDPEVVEKIGIW
                     LRAAAALADARKMKVVRFGDNMRDVAVTEGNKVSAQLKLGYSVYGYGVGDLVKYVNSA
                     SPAAIKKKIKEYGEEYRLTKAVASSESLYEAAKIEVGLENFLKEGDFKAFTTTFEDLH
                     GLKQLPGLAVQRLMSKGYGFGAEGDWKTAALVRSMKVMASGLKGGTSFMEDYTYHFDP
                     AGMRVLGAHMLEVCPSIAKGKPSVEIHQLSIGGREDPARLVFSAQTGRAINVSVIDLG
                     NRFRMIVNDVESVECPDMPKLPVARVLWKPLPDLKTAAAAWIYAGGAHHTGFSLALTA
                     EHMEDFAVMTGIEFLHIGEKCDLNQFRKELMWNEAYYHIAGGIS"
     gene            1535..2236
                     /locus_tag="PLG42_04045"
     CDS             1535..2236
                     /locus_tag="PLG42_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU30417.1"
                     /translation="MKIYSGIPKHLVAVDCIIFGYDIMEKELKLLLIKRGFEPAKGKW
                     SLAGGFVYENESLEEAAARVLYNLTGLRNVYMEQNYTYGEVDRDPGARVISTSFYALI
                     KIQDIDRELAEKNGAHWRSISRLPELIFDHTRMVERALNELRQQAKVKPVGFELLPEK
                     FTLVQLQDLYEAIYQRKIDKRNFRKKILSMNLLEKLDEKEKITSKRGAWYYRFIPEKY
                     EVFTQKGFYFNLDVS"
     gene            2276..2968
                     /locus_tag="PLG42_04050"
     CDS             2276..2968
                     /locus_tag="PLG42_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOU30418.1"
                     /translation="MLEKFKEQVLEANLEIVKQGLVIQSWGNVSGRDPETGYIVIKPS
                     GVSYSKMKASDMVVLDLLGNIIEGKMKPSTDTPTHIILYRTWPSVGGIVHTHSTYATA
                     WAQAGRGIPPLGTTHADTFYGEVPCTRKLNPEEIKSDYEANTGRVIVEQIGSTDPLFM
                     PSVLVNCHGPFSWGKNADEAVYNAVTLEEVARMAFYTVLLGNQQAIDRALLDKHFLRK
                     HGKDAYYGQEKE"
     gene            2985..4661
                     /locus_tag="PLG42_04055"
                     /pseudo
     CDS             2985..4661
                     /locus_tag="PLG42_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            4692..6263
                     /locus_tag="PLG42_04060"
     CDS             4692..6263
                     /locus_tag="PLG42_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU30419.1"
                     /translation="MERKKILTLVVLFLGFLTTAVYPQKTGNETVNQFVIVANNPGPV
                     ISKDIYGHFSEHLGTCIYGGIWVGPDSKIPNTYGIRNDVLFALREIKVPNLRWPGGCF
                     ADTYHWRDGIGPQSKRASIINIHWGGVTEDNSFGTHEFMKLTELLGCDAYINGNVGSG
                     TVQEMAEWVEYLTSDAESPMTRLRKENGRETPWKVRYWAIGNENWGCGGNMTDEFYAS
                     VMRQYSTYLNNYGGNRLYKIACGPYGDNFQWTETLMKDPSTRNMFQGISIHYYSVVDG
                     WNYKGSATNFDEREWFETFRLNYDIERIIIGHKNIMDRYDPNKIKGLIVDEWGNWFNV
                     EPGTNPGFLYQQNTLRDAITAAIHLNIFNHHADRVKMANLAQMVNVLQAVILTKDDKM
                     VLTPTYYVFKMFKNHQDARLLKTDLKCENYTYGDKKIPAISASASMGKDGTILITMAN
                     LNPSKDITVTCPVIGATVKTISGQVLTAREMNAHNTFENPDNVKPAEFKGYKYSNGVL
                     TINMPAKSVVALELK"
     gene            6265..8250
                     /locus_tag="PLG42_04065"
     CDS             6265..8250
                     /locus_tag="PLG42_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOU30420.1"
                     /translation="MKNLLRVLLVINIILAATGCTGHKTGDYPVKPVPFTSVKLTDDF
                     WAPRIKKNAEITIPIAFGYCESTGRIKNFEIAGGLDTGKFQTLYPFDDSDVFKIIEGA
                     SYSLQTFPDPKLEAYLDTLIWKIGLAQEDDGYLYTNRTIAEMHGGEGLHEWASPRRWE
                     LDSVLSHELYNIGHLYEAAVAHYQATGKRTLLDIAMKSADLVAKDFGYDRLKVYPGHQ
                     VIEMGLAKLYRITGDEKYLDLAKFFLDIRGPEGQEYNQAHILPVDQTEAVGHAVRATY
                     MYSGMADIAALKNDNRYLNAITRIWEDLVYRKMYVTGGIGASGGNEGFAGPYVLPNMT
                     AYCETCASIGNIFFNHRLFLFHGDAKYYDIIEKTLYNSMLSGVSLSADRFFYPNPLES
                     KGQHKRQAWFGCACCPSNIARFMPAIPGYIYAVADKDIYINLYASNRAKINAGKQNIV
                     IVQDTQYPWEGRVEISVEPEKETNFTLRLRIPGWVRNEAIPGRLYRFTNTSGEKFSLT
                     VNGIVVKYSIDKGYAVIKRKWKKGDRVVVDFPMPVRVICADERVKDDAGRVAFQRGPF
                     VYCAEWPDNNTGNVRNLIIDTTAVLSTMFRNDLLGGVQIITVQGRQATKPSEGEIRFM
                     EPEPVTLIPYAYWNNRGPGQMIVWLPVKSEAAGFLKD"
     gene            8258..9490
                     /locus_tag="PLG42_04070"
     CDS             8258..9490
                     /locus_tag="PLG42_04070"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOU30421.1"
                     /translation="MKGNFFDSPQENVIFVAKNNDMLKKYIVCLPEKEGTVINEWEQC
                     LKQLKEFIKDGYRPVKFNVFIDVTEYNSFIEIRRQIIDSLLDALGTESPAVNVTVHPP
                     EQPFKVVVEAMFAVGRGASYKTKLFNGLPYVIVEQSQYRELWAAGVGEEFPESLKHAA
                     YFAFDNVLEILQLEGMSFDNVVRQWNYIGNILEVRDEYQNYQIFNDIRSECYRKYRTV
                     KHFPSATGVGMKMPGVFLDICAVDSGEELLIMPVSNPLQINAYEYGQQVLKGLPLNGT
                     KPKNPPQFERALLVENRSNLILFISGTASIIGQETIGKGDIAKQVTVTINNIKTLSEP
                     ERIRKTSGRNFSGSGRYLLLRVYIRRKEDFSIVKEITEKSFPGVPAVYIQADICRDDL
                     LTETEAVMVFEPDSKAGN"
BASE COUNT         3004 a         1879 c         2300 g         2446 t
ORIGIN      
        1 acctcaggaa gtttgaagca tggttcgtga ccggcagcca gcacctgtac ggtcccgaaa
       61 cattgaaaca ggtagcaacc gattcgcatc agattgctgc agaactcgac aaaaaccccg
      121 caataccggt aaagattata ttcaaaccgg ttctcacaac gcctgacgcc atctttgaac
      181 tttgctctga agccaatagc gctcccaact gcataggatt aatcacatgg atgcatacat
      241 tctctccagc caagatgtgg ataagaggtc tttcagcact taacaaaccc tttctgcacc
      301 tgcatacaca gctcaacaga gatatcccgt ggaaaacaat tgatatggat ttcatgaatc
      361 tgaaccaatc ggctcacggc gacagggagt ttggtttcat aacaaccagg atgaggctta
      421 accggaaagt tgttgtagga cattacaaag acccggaagt ggtggaaaaa ataggcatct
      481 ggctccgagc cgcagcagct ttagccgatg cacgcaaaat gaaagtcgta cggttcgggg
      541 ataacatgag ggatgtagct gttacagaag gaaacaaggt gagtgctcag ttaaaacttg
      601 gatattctgt atacggatat ggtgtgggag accttgtaaa atacgtcaat tctgcttcac
      661 cagcagccat aaagaaaaag atcaaagagt acggggaaga atacagactg acgaaagcag
      721 tagcttcttc tgaatcgctt tatgaagctg caaagatcga agttggactt gagaactttc
      781 tgaaagaggg tgatttcaag gcttttacca ctactttcga agaccttcac ggtttgaagc
      841 agttacccgg gctggcagtt cagcgactca tgtcaaaagg atatggtttc ggtgccgagg
      901 gtgactggaa aacagctgca cttgtgcgct ctatgaaggt tatggcttcc ggacttaaag
      961 gtggtacctc atttatggaa gactatacct atcatttcga cccggcagga atgagagtgc
     1021 tcggtgccca tatgcttgaa gtgtgcccgt caatagcaaa aggcaaaccc tctgttgaaa
     1081 tacaccagct ttctatcgga ggcagggaag atccggctcg cctcgtattc agcgcccaga
     1141 caggcagggc aataaacgtt tcggtaatag atctgggcaa cagattcagg atgatagtaa
     1201 atgatgttga gtcggttgaa tgccccgata tgcccaaact gcccgttgcc agagtactct
     1261 ggaaacctct tcctgacctc aaaacagctg cggcagcatg gatatatgcc ggtggagcac
     1321 accataccgg attcagcctt gctcttaccg ccgaacatat ggaagacttc gccgttatga
     1381 caggcataga attcctccat atcggtgaaa aatgcgacct caaccagttc agaaaagaac
     1441 tcatgtggaa tgaagcatac tatcatattg cagggggcat cagttaaccc cctgcatgct
     1501 tctattcaac atcctaacaa agcacctgaa tcatatgaaa atttattcag gaataccaaa
     1561 acacctcgta gcggttgact gcatcatttt cggatacgac ataatggaga aagaactgaa
     1621 actgcttctg ataaagaggg gtttcgagcc tgcaaaaggt aaatggtcgc tggcaggagg
     1681 atttgtttat gaaaatgaaa gcctggaaga ggctgcagcc agagtactct acaatctgac
     1741 cggactcaga aatgtatata tggaacagaa ttatacatat ggtgaggttg acagagatcc
     1801 gggagcaaga gttatttcaa cttcattcta cgcactgata aaaattcagg atatagatag
     1861 agaacttgcc gaaaagaatg gagctcactg gcgctccatt tcaagacttc cggaacttat
     1921 tttcgaccat acaaggatgg tggaaagagc acttaacgag cttcgccagc aagctaaagt
     1981 gaaacctgtg ggatttgaat tgctacctga aaaattcaca cttgtacagc ttcaggatct
     2041 ttacgaggct atataccaga gaaaaattga taaaagaaat tttcgcaaaa aaattctctc
     2101 aatgaacctg ctcgaaaaac tcgacgaaaa ggagaagata acatccaaaa ggggtgcatg
     2161 gtattacagg ttcatccccg aaaaatacga agtgtttaca cagaagggtt tttacttcaa
     2221 ccttgatgta agctgaaaaa acttactgaa atatatttaa ggacattata aaaatatgct
     2281 tgaaaaattc aaagaacaag ttcttgaagc caacctggaa attgtaaaac aggggctagt
     2341 tatccaaagc tggggtaatg taagcggtcg tgaccctgaa acaggttata ttgtgataaa
     2401 accctcggga gtgagctatt caaagatgaa ggcatctgac atggtagtac ttgaccttct
     2461 agggaatata attgaaggta aaatgaaacc gtcaactgat actcccactc atatcattct
     2521 gtatcgaaca tggccttcgg ttggcgggat agttcatact cactcaacct atgcaactgc
     2581 gtgggctcag gcgggaagag ggattcctcc tctgggtacg acccatgccg acaccttcta
     2641 tggcgaagtg ccatgcaccc gtaaacttaa ccctgaggaa ataaaatcag attatgaggc
     2701 caataccggc agggtgattg ttgaacagat tggctcaacc gaccctcttt ttatgccatc
     2761 ggttctcgta aactgccatg gaccttttag ctggggtaaa aatgctgatg aggcagttta
     2821 caatgcagtc acactcgagg aggtggcaag aatggctttc tatacagtgc ttctaggaaa
     2881 tcaacaggct attgacagag ctttgcttga taaacatttt ctgaggaaac acggcaaaga
     2941 tgcgtattac ggacaggaaa aagaataaaa aaacagaaaa gaatatggct gacaaatatg
     3001 taataggtct cgattacgga accgattcgg ttcgcactgt tgtagtcaat gcgcttaatg
     3061 gcgagatagt tggaacatct gtatttgaat accctcgatg gaagaagggt ttatactgca
     3121 atcctgccat caatcagttc cgccagcacc cactggatta tattgagggc atggaggttt
     3181 ctgtaaggga ggcgttacgt ggacttgggc gggatgtaat agacaacata gaaggtataa
     3241 ctgttgacac tacaggctca acacctgttg ctgtcgacag gcagggagta cctctttcgc
     3301 ttaagaaaga atttaaggaa gagcctgatg caatgtttat tctctggaag gaccacacag
     3361 cagttgatga agcagctgag ataaaccggc ttgccagaac atggggtggt gaagattata
     3421 cgaaatatga aggtggagtc tattaatcgg agtggttctg ggcaaagatt cttcacgtgc
     3481 ttcgcaggaa tgaaaaagtc agaagaaatg catggtcgtg ggttgagcac tgcgactgga
     3541 tacctgcgct tcttactggt gagactgacc ctcttaagat aaaaagaagc cgctgtgcag
     3601 cagggcacaa ggcaatgtgg caccccgatt tcaacggact gccctctgaa gagtttctgg
     3661 taaggcttga tccattactt gccggattga gggagaggct ctataaagaa acatatacct
     3721 gtgatgtggt tgtgggtacc cttacaaggg aatgggcaga gaggctggga ctttcgacac
     3781 gggtaaaggt tggagcagga gccttcgatg cccatctggg agctgtggga ggagagatta
     3841 aaccatacca gctggttaaa gtaatgggca cttctacatg cgatatgctc atcgtcccga
     3901 tggaagaggc gcgcgataag cttgtaaaag gtatatgtgg gcaggttgat gggtcaatag
     3961 tgccgggaat gctcggattg gaggccgggc agtcggcgtt cggcgatgta tatgcctggt
     4021 tcagccgcct gctgatgtgg cctcttgaaa cgattattcc ccggatgagt tggctcgacg
     4081 aaaaaacaat agagagaatc agaaatgaat catccgataa aattattgcc gaactgagcc
     4141 atcatgctct gactcttaat gatgaggaaa cagcactgct ggctctcgac tggctcaatg
     4201 ggcgacgtac tccgtttgcg aatcaggaat tgaaaggtgc cattacagga ctaactcttg
     4261 gctctgacgc cccacgcatc ttcaaagcac tcgtggaggc aacagccttc ggcgccaaaa
     4321 tgataaacga ccgcttcatc gaagaaggtc taagaataga aggagttatt gctattggcg
     4381 gagtggcaaa gaaaaaccca tatgtaatgc aggtggtggc cgacgtcctg aatgtgccta
     4441 tcaaagtggc acgttcggaa caaactgtgg cacttggctc agcaatggct gcctcggttg
     4501 tagctggcat acaccccgat atcccaacag cacaaaaagc catgggcggc gggtttgaga
     4561 aagaatatgt gcccaaccca cataaagctg ataaatacag aatactttat gagaaatata
     4621 agaaactggg caccttcatt gaaaatgagc ttacagagtg aaaacaaata ataataaaaa
     4681 tattatatgc tatggaaaga aaaaagattt taaccctggt agtactcttt ctgggattcc
     4741 tgacaacagc tgtttatccg cagaaaacag gaaatgaaac cgtaaatcag tttgtgatag
     4801 ttgcaaataa tcccggtccg gtaatatcaa aagacatata tgggcacttt tcggaacatc
     4861 taggcacctg tatttatgga ggtatatggg taggacccga ttcaaaaatt ccaaatacct
     4921 acggtataag gaatgatgtt ctttttgcac tgcgtgagat taaggtgcca aatcttcgct
     4981 ggcccggagg atgttttgcc gatacttacc actggcgcga tggaattggt ccacagtcaa
     5041 agagagcctc tataataaac atccactggg gcggggtgac cgaagataat tccttcggaa
     5101 cacacgagtt tatgaagctc accgaactgc ttggatgcga tgcttatatc aacgggaatg
     5161 tgggttcagg aacagttcag gagatggcag agtgggttga atatctcaca agtgatgctg
     5221 agagtcccat gacaagactc cgcaaggaga acggcaggga aacaccatgg aaggtcaggt
     5281 actgggcgat aggcaacgaa aactggggat gcggcggaaa tatgacagat gaattttatg
     5341 catcagtcat gcgccagtat tcgacatacc ttaataacta tggaggtaac cgtctttaca
     5401 aaatagcctg cggaccctac ggcgacaatt ttcagtggac ggaaactcta atgaaagatc
     5461 cttccaccag aaacatgttc cagggtatat ccattcatta ctattcagtg gttgacggat
     5521 ggaactataa aggctccgct accaatttcg acgagaggga atggttcgaa actttccgcc
     5581 tcaattacga catcgaaaga ataataattg gccataagaa tattatggat cgctacgacc
     5641 cgaataaaat aaaggggttg attgttgatg aatggggaaa ctggtttaat gtcgaacccg
     5701 gtacaaatcc tggttttctg tatcagcaga acacattacg cgatgccatt acagccgcaa
     5761 tacacctgaa tattttcaac catcatgctg acagggttaa gatggctaac cttgcacaga
     5821 tggtcaacgt tcttcaggct gtaattctta cgaaggatga caaaatggta ctgaccccaa
     5881 catattatgt attcaagatg tttaaaaacc atcaggatgc aaggctcctg aaaactgacc
     5941 tgaaatgcga gaattatact tatggagata aaaagattcc agccatttcg gcatcagctt
     6001 cgatgggtaa agacggtaca atactcatta caatggcaaa cctaaatccc tcaaaggata
     6061 taacagtgac ctgccctgta ataggtgcaa cagtaaaaac tatttcaggt caggtattaa
     6121 ctgcccgtga gatgaatgcg cataatactt tcgagaatcc cgacaatgtt aagccagccg
     6181 aattcaaggg ttataaatac agcaacggcg ttcttacaat aaacatgccg gcaaaatcag
     6241 tggtagcact tgaattgaaa tgatatgaag aatcttttaa gggttttgct ggtcattaat
     6301 attatcctgg cagcaaccgg atgcacaggg cacaaaactg gtgattaccc tgttaagcct
     6361 gtccctttta cttcagtgaa gcttactgat gatttctggg caccaaggat aaaaaagaat
     6421 gctgaaatca caatcccgat agcatttggc tattgtgaat cgacaggcag gattaagaat
     6481 ttcgaaattg caggaggatt ggataccgga aagttccaga ccctttatcc ttttgatgat
     6541 tcagatgtct tcaaaatcat tgagggtgca tcttattcgc ttcagacttt tcccgatccg
     6601 aagctggagg catatctcga tacacttatc tggaaaatag gcctggcaca ggaagatgat
     6661 gggtatctct ataccaaccg caccatagct gagatgcacg gtggtgaagg attacatgaa
     6721 tgggcaagtc cccggcgctg ggaactcgat tcagtcctga gtcatgaact ctacaacatc
     6781 ggacatctgt acgaagctgc cgtagcccat taccaggcaa ccggaaagag gacacttctt
     6841 gatattgcca tgaaatcggc tgacctggtc gcgaaagatt ttggatacga caggcttaaa
     6901 gtttatcccg gtcaccaggt tatcgagatg ggactggcaa agctatacag aatcacaggt
     6961 gatgagaaat atctcgatct ggcaaaattt ttcctcgata ttcggggtcc agaggggcag
     7021 gaatataatc aggcacacat tcttcctgtt gaccagacgg aagcagtggg acatgcggtg
     7081 agggcaacat atatgtactc gggaatggct gatatcgcag cgcttaaaaa cgacaaccgg
     7141 tatctgaatg cgataacgcg tatatgggag gatctggttt atagaaaaat gtatgtgacc
     7201 ggtggaatag gagcttcggg aggaaatgaa gggtttgcag gtccttatgt actgcctaac
     7261 atgacagcct attgtgaaac atgtgcttca ataggtaata tattcttcaa tcacagactc
     7321 ttcctctttc acggcgatgc aaaatattat gacataattg aaaaaacact ttataattca
     7381 atgctttcgg gcgtctcgct ttcagccgac agattttttt atcccaatcc gctcgaatct
     7441 aaaggacagc ataaacgcca ggcatggttc ggatgcgctt gctgcccctc taatattgca
     7501 aggttcatgc ctgctatacc aggctatatc tatgctgttg ccgataagga tatctatata
     7561 aatctttatg cttctaatag agcaaaaatt aatgccggaa aacaaaacat cgttattgtt
     7621 caggacacac aatacccatg ggagggtaga gtggaaatat ctgtagaacc tgaaaaagag
     7681 acaaatttta ctttgaggtt aagaataccc gggtgggtaa gaaatgaggc tataccggga
     7741 cgattataca gatttacaaa cacttcaggt gaaaaattct ctttgacagt aaacggtata
     7801 gttgtaaaat attcaattga taaaggatat gcggttataa aaagaaaatg gaagaaagga
     7861 gacagggttg ttgtagattt ccccatgccg gttagagtga tatgtgctga tgaaagagta
     7921 aaagatgatg caggaagagt tgcttttcag agaggacctt ttgtctattg tgctgaatgg
     7981 cccgataaca acacaggcaa cgtgcgaaat cttatcattg atacaaccgc agttctatcc
     8041 accatgttcc gtaacgacct gcttggaggt gtgcaaataa ttactgttca ggggcgacag
     8101 gcaacaaagc cgtccgaggg tgaaataagg tttatggagc ctgaaccggt cactctcatt
     8161 ccttatgcat actggaacaa tcgtggaccg gggcagatga ttgtatggct gcctgttaaa
     8221 tctgaagcag ccggatttct gaaagattga gtttctcgtg aaaggaaact tctttgattc
     8281 gccacaggaa aatgttattt ttgttgcgaa aaataatgat atgctgaaga aatatattgt
     8341 ttgcttgcct gaaaaagagg gaacagtaat aaatgaatgg gaacaatgtc tgaaacaact
     8401 gaaggaattt attaaagacg gataccggcc tgtaaagttt aatgtattta ttgatgttac
     8461 agaatataat tcgtttatag aaattcgaag gcagataata gattcactcc ttgatgcact
     8521 gggcaccgaa agccctgctg tcaatgtaac tgttcaccct cctgaacaac cgtttaaagt
     8581 agttgttgag gcaatgtttg ctgttggcag aggggcttca tacaaaacaa aattgtttaa
     8641 tggattacca tatgtaattg ttgaacaatc acaatacagg gaattgtggg cagccggtgt
     8701 aggagaagaa tttcctgaaa gtttaaaaca tgctgcatat ttcgcttttg ataatgttct
     8761 tgaaatttta cagcttgaag gaatgtcatt cgataatgtt gtaaggcaat ggaattatat
     8821 cggaaacatt cttgaagtca gggacgaata tcaaaattat cagatattca acgatataag
     8881 aagtgagtgt tacagaaaat accggactgt aaaacatttt ccttcggcaa ccggagtggg
     8941 tatgaaaatg ccgggagtat ttcttgatat ttgtgcagtt gattcaggag aagaattgct
     9001 gataatgcct gttagtaatc cacttcagat taatgcttat gaatatggtc agcaggtact
     9061 gaaaggctta ccgcttaatg gtacaaagcc caaaaatcct cctcagtttg aaagagcact
     9121 gctggttgaa aacagaagca atctgatact ttttatttcg ggaacggctt ctataattgg
     9181 tcaggaaaca atagggaaag gggatatagc aaagcaggta acagtaacta taaataatat
     9241 caaaacgctc agcgaacctg agcgcatacg aaagacatct ggacgtaatt tttcaggttc
     9301 aggcagatat ctgttgctga gggtttatat aaggagaaag gaagatttta gtattgttaa
     9361 ggaaataaca gaaaagagtt ttccgggtgt acctgcagtt tatattcagg ctgatatctg
     9421 ccgtgatgat ttgcttacag aaactgaagc agtaatggtt tttgagcctg atagcaaggc
     9481 aggtaactga aatgtctcat ataagcacct gtgaaaaata cctgtaattg acagtttatc
     9541 gggagttttt aacataagat tttattttta aattagattt catataaaat atttaccaat
     9601 gaaaaaagca gttgttttat cagttatgg
//
LOCUS       DAOSNB010000062         9547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7128_length_9547_cov_4.654973, whole genome shotgun sequence.
ACCESSION   DAOSNB010000062 DAOSNB010000000
VERSION     DAOSNB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7128_length_9547_cov_4.654973"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PLG42_04075"
     CDS             <1..993
                     /locus_tag="PLG42_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOU30422.1"
                     /translation="QFQENGFRLYSFLLKPVEEYLISEYLVISPDGKLSFFPFDIMIM
                     SEKRDTSLYYNILDYVTKKYDISYTYSATLLSETRRRMPSFFNNAVTFAPSYDNLIVD
                     SVFRSENYNDLPPLVYAQQEASYVSKLIPGKLFMNEKATLENYKAEAGKFDVVHLAMH
                     TIVDGASPANSGMIFYNATVDSAEDVYLKPYEVYGIPLNAKMVVLSSCFTGTGTLYAG
                     EGVLSLARGFIVSGSKSVVMSLWEIDDMAGASIIKSYYKNLKSGMRKSRALKKARMDY
                     LERADMRLSHPYYWSTLVIYGDDSPLYISWYIKTVLLIVLSLVLIITIRYFKKR"
     gene            complement(975..1538)
                     /locus_tag="PLG42_04080"
     CDS             complement(975..1538)
                     /locus_tag="PLG42_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU30423.1"
                     /translation="MGKLSDKDIIEGVRRQDEKVLNYLYDNYFKLVKNHVIKNNGTED
                     DASDVFQETIILLYQKICENNLELTSDLRGFFFGIARNVWNNLFRQKSKTEELHNIDI
                     VEENDEEVSDQLFMKIMTRAFNKLKPECQEVLKMFYDGLSFEEIAQKMNYKNEIYARR
                     KKYLCKEALIEIIKSDPEYIEYQRFLK"
     gene            1702..3888
                     /locus_tag="PLG42_04085"
     CDS             1702..3888
                     /locus_tag="PLG42_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU30424.1"
                     /translation="MSFPFVRQPDAMDCGPACLKMIAGFYGKNFSLESLRKRCYITRE
                     GVSFLGLSEAADTLGFRTIGVKIPFSTFIKNVPLPCIVHWKQRHFIVVYRVSPKYIWV
                     ADPALGLLKYSHQEFINNWSGTVINGEPVGLVLIMEPTPAFYETEEEKEQKKGFLFLW
                     KYFRLYKKYFYQLFLGLFLGSLIQLVIPFITQSVIDIGINNSDIGFIYLILFAQLALV
                     AGRFSVEYIRNWLLLHIGARINVAVVSGFLHKLMALPVSFFDTKLTGDILQRIDDNNR
                     IEEFLTSASLSIMFSFFNLIVFGIVLAIFNLKILLIFLAGTLAYIGWVSLFMKSRAIL
                     DVQRFRQMSTTNSKLIQMVNGMQEIKLTQSELARRWEWENLQAGLFHLRVKGLNLLQH
                     QSAGATLIHEVTNILITLVSATTVIKGEMTLGMMLAILFIIGQLNLPVTQIIYFFRVS
                     QDAKLSLDRLSEVHSMDDEEPEPEAKVKKMPERKDIYINNLSYQYEGPRSPFVLKDID
                     LVIKENNVTAIVGASGSGKTTLLKMILGFYKPVTGEIVVGDTKLENISLKTWRKKVGA
                     VMQDGYIFADTIASNIAPGVETIDEERLYRAAEIANIRGFIESLPLSYNTKVGMNGHG
                     LSECQKQRLLIARVVYKDPEIIIFDEATNSLDAGSEKVIVENLKSFFKNKTVIIVAHR
                     LSTVRDADKIVVLDNGRIVESGTHNELIGRQGAYFNLIKNQLELGN"
     gene            3915..5198
                     /locus_tag="PLG42_04090"
     CDS             3915..5198
                     /locus_tag="PLG42_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU30425.1"
                     /translation="MKKHEIQYSEPVREIMGNPPGKILRWGTMIIFAIFVLFISFAWL
                     IKYPDIVPAQVEITTENPPASIMSKTTGRIMHLMVSDKQQVKAGEMLAVMENPASFEE
                     VRKLKNFIDTFNLRSDVKIETIPDFYNLGELQTPYETFRKSVSDIDNFIKNDYYNARI
                     NAVNEELRELKTYTARLQENEKFYNENYLIELNKFRRDSALNAGKSIAPADYENSKQA
                     LIRQQIELQKVRLDISAKNIEMINKRQLIEEYSIKRSEELDKLYSNAREALKNLRAEL
                     SIWENTYLLVSPVEGQVTFSRYWSVNQLVKKDETVMSVVPDNPGNYIGKIYLKMYKSG
                     KVREGQIVNIKLNGYPYMEYGMLQGVIRSKALVPAGDSYLIEVELKNGLTTLYNRTLE
                     FTQNMTGVAEIITDDRSLLEKMIYPFRYLISRNKR"
     gene            complement(5206..5379)
                     /locus_tag="PLG42_04095"
     CDS             complement(5206..5379)
                     /locus_tag="PLG42_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30426.1"
                     /translation="MKDPVSIFITVTVFIIVAIRLYLRYKEKGTSGANKAKSKQDLSS
                     SPQEEEYEPYKNK"
     gene            5607..7799
                     /locus_tag="PLG42_04100"
     CDS             5607..7799
                     /locus_tag="PLG42_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOU30427.1"
                     /translation="MIEKDPNNPVLNEQFAATEGAGESTGNVPGGNIEPPVDFSVNET
                     EKPESLTSDEKKNRDGEVDMTEFSVPEKENEEKINTGTESVPVVQSTETAEASEDLSD
                     SGEHISGGEKVKRPRKKSGEKKSVSPVIIPETSLAAERQGDIPEDYKDIELPPVDYSG
                     YSKEKLVETLELIIDNRPPNEIKNDVERIKSLFYKKIRKENEERKKKFLEEGGKAEDY
                     RVLVDPLEVKVKELLEKYKSKKSDFGKIQEAEKLENLRKKYEIIEKIKDLVNREESIN
                     KTFQEFRALQNEWHAVGIVPQSALKDLWENYHHAVEIFYDYIKINKELRDLDLKKNLE
                     AKVRLCEKAEELMLEPNPVTAFKLLQDLHNAWREIGPVPHESKNEIWERFKEATAKIN
                     KRHHEFFEKQKEEQKKNLEAKIALCEKVEEINREELKTFKDFEEKAKIVIDLQKMWRT
                     IGFAPKKQNNKIYQRFREACDAFFEKKRNFYADSKEMQMNNLQRKTDLCIQAEALQES
                     TDWKATTEALIRLQKEWKEIGPVPRKYSDKIWKRFRKACDHFFNRKAEYFASLDSTYE
                     ENLKAKEAIIAELENFEPGDDVQEAFNKLKEIQKRWTDIGFVPFNRKEEINNRYRNAL
                     NKQFDKLKIDEVDKSILKYKTKLETLKSNPKALRKLSNEREKFISKIKQLENDIVLWE
                     NNIGFFAKSPNAETMIKEVEEKIKNAKKTIKTLEEKVKLIDRINVEEL"
     gene            7820..9442
                     /locus_tag="PLG42_04105"
     CDS             7820..9442
                     /locus_tag="PLG42_04105"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOU30428.1"
                     /translation="MADTKYVFVTGGVTSSLGKGIISASLAKLLQSRGYSVTIQKLDP
                     YINVDPGTLNPYEHGECYVTIDGAETDLDLGHYERFLNVTTSQANNVTTGKVYQTVIE
                     KERRGDYLGKTVQVIPHITDEIKRRIKLLASSKKFDIVITEIGGTVGDIESLPYIESV
                     RQLRWEMGQGNCVVIHLTLVPYLSATGEQKTKPTQHSVKALLEAGIQPDVLVLRTEKS
                     ISSDIRKKVALFCNVEEKAVIESVNVSTIYEVPLRMLEEGLDQVVLQKLNLPVKNKPD
                     MSEWETFVHKLNFPKRKVNIALVGKYVELHDSYKSIHESFIHGGAANDCSVNIGYFNS
                     ENISDTNVAELMNDIDGILIAPGFGSRGVEGKISTVKYARINNVPFFGICLGMQCAVI
                     EFARNVIGYEDAHSTEMDSDTKHPVIDLMEEQKSITEKGGTMRLGGYRCILSPDSLSY
                     KAYGKKEIIERHRHRYEFNNTYLEEFKQHGMLMAGINPESDLVEIIELPSHRWFVGVQ
                     FHPEYSSTVMNPHPLFVSFIRACIEFADFKRG"
BASE COUNT         3237 a         1577 c         1966 g         2767 t
ORIGIN      
        1 caatttcagg aaaatggatt cagactatat tcgtttctgc ttaagccggt tgaagaatat
       61 cttatttctg aatatctggt gatttcaccc gatggtaagc tgtccttttt cccgttcgac
      121 ataatgataa tgtcagaaaa aagagataca agcctttatt ataatattct tgactatgtc
      181 acaaaaaaat acgatatatc ctatacttat tctgcaacgc ttctatcgga aaccagaaga
      241 agaatgcctt ctttttttaa taatgcagtc acttttgcac cttcatacga caatttaatt
      301 gttgattcag tttttcggtc tgaaaattac aatgatttac cacctcttgt atatgcccag
      361 caagaagcat cttacgtgtc taaattaata cctggtaagc tttttatgaa tgagaaagct
      421 actttggaga attataaagc agaggccgga aagtttgatg ttgtgcacct tgccatgcat
      481 accatagtcg acggggcatc acctgcaaat tcaggaatga tattttataa tgctactgtg
      541 gattcggctg aagatgttta tcttaagccc tacgaagttt acggaatacc tttgaatgca
      601 aaaatggttg ttttaagttc ctgttttacc ggaacaggaa ctctctatgc aggcgaagga
      661 gtgctcagtc tggcaagagg ctttatagta tctggaagta aatcagtagt gatgtctcta
      721 tgggagatag acgatatggc tggtgcctcg ataataaaat cgtattacaa aaaccttaaa
      781 tcgggaatga gaaagagtcg ggcacttaaa aaggccagaa tggattatct ggaaagagca
      841 gatatgcgat tatcccatcc atattattgg tcaacacttg ttatatatgg cgacgattcc
      901 ccactttata tctcatggta tattaaaaca gtactcctta ttgttctgtc cctggtcctg
      961 attatcacta ttcgctattt taaaaagcgt tgatattcaa tgtattctgg atcagatttt
     1021 atgatttcaa tcaaagcttc cttgcacaga tatttttttc tgcgagcata tatctcattt
     1081 ttataattca ttttctgagc tatttcttca aatgagagtc catcataaaa catttttaat
     1141 acttcctggc actcaggttt aagtttatta aatgctcttg tcatgatctt cataaaaagc
     1201 tgatctgata cttcttcatc attttcttca acaatatcaa tattatgaag ctcttcggtt
     1261 ttggacttct gtcggaaaag attattccat acatttcgtg cgataccaaa gaaaaacccc
     1321 ctcaggtcac tggtcagttc cagattgttt tcacatattt tctgatagag aagaatgata
     1381 gtttcctgaa aaacatctga agcatcgtct tcagtaccgt tattcttaat tacatgattt
     1441 ttaaccagct taaaatagtt gtcgtacaaa taattgagaa ccttttcgtc ctgacggcga
     1501 acaccttcta ttatatcctt atctgataat ttccccaaca atcccctgtt gataccttaa
     1561 ttgattgaca aatatactat ttccaaaggt aatccacagt aaatttattc cactgattat
     1621 agtttttata aaatattgaa attttacatt gtcaaaaata aatatcttta ccctaactaa
     1681 aaaactatct gtatttgagt aatgtcattt ccgtttgtca ggcaaccaga tgcaatggat
     1741 tgtggacctg cctgccttaa gatgatagca ggtttttatg gaaagaattt ttcacttgaa
     1801 tctctccgta aaagatgcta cattaccagg gagggtgtgt catttcttgg tctgtcggaa
     1861 gcagcagata cactgggttt tagaaccata ggagtaaaaa tacctttcag tacatttatc
     1921 aaaaatgtcc cattgccatg tatagttcac tggaaacaaa gacacttcat tgttgtatac
     1981 agagtttcac cgaagtatat atgggttgcc gatccggcac tgggtctgct taaatacagc
     2041 catcaggaat tcattaataa ttggtcagga acagttatta acggagagcc agtgggactg
     2101 gttctgataa tggaacctac tccggcattt tatgaaactg aagaggagaa ggaacaaaaa
     2161 aaaggtttcc tgtttctctg gaagtatttc agattataca agaagtattt ttatcagtta
     2221 ttcctcggac ttttcctggg cagtcttatt caactggtta ttccttttat tacccagtca
     2281 gttattgata taggtatcaa taatagcgat ataggattta tttatcttat tctttttgct
     2341 caacttgcac ttgtggcagg aaggttttct gttgagtata tacgtaactg gctattgctt
     2401 catataggtg caagaataaa tgttgcagtg gtatcaggtt ttctgcacaa actgatggct
     2461 ctgcctgtct cattctttga tacaaaatta acaggcgaca tactgcagag aatagatgac
     2521 aacaacagga tagaagaatt tctcacctca gcatctctga gcataatgtt ttcatttttc
     2581 aacctgattg tttttggaat agttctggct attttcaatt taaagattct tttgattttt
     2641 cttgccggta ctctggcata tattggctgg gtatctcttt tcatgaaatc ccgagctatt
     2701 cttgatgtcc agaggttcag acagatgtca acaacaaaca gcaaactcat ccagatggtc
     2761 aatggcatgc aggaaattaa actcactcag agtgaacttg cacgaaggtg ggaatgggaa
     2821 aaccttcagg caggtctttt tcatctgagg gttaaagggt tgaacctcct gcagcatcag
     2881 tcggccggag ctacattgat tcatgaggtc actaatattt tgattacact tgtttcggca
     2941 acaacggtta ttaagggcga aatgacactc ggtatgatgc ttgcgattct atttattatc
     3001 ggtcagttaa acttaccagt tactcagata atttatttct tcagagtgtc gcaggatgca
     3061 aaacttagtc ttgatagatt atctgaagtt cattcaatgg atgacgaaga acctgagccc
     3121 gaagccaaag taaagaaaat gcccgagagg aaagatattt atatcaataa tctttcatat
     3181 caatacgagg gaccaagatc tccctttgta cttaaagata ttgatcttgt gattaaagag
     3241 aataatgtaa cggcaattgt tggtgccagt ggaagcggca aaacaaccct gcttaagatg
     3301 atacttggtt tttataaacc agttaccgga gaaatagttg taggagatac aaaactggaa
     3361 aacataagcc tcaaaacatg gaggaaaaag gtaggtgcgg ttatgcagga cggttacatc
     3421 tttgccgata caattgcatc taacattgct ccgggggttg aaactataga cgaggagaga
     3481 ctttacagag cagccgagat tgccaatatt agaggtttca ttgagtcgct gccattaagc
     3541 tataatacaa aagttgggat gaatgggcat ggtttaagtg aatgtcagaa gcaaagactt
     3601 cttatagcac gggtagtgta taaagatcct gagataatta tttttgatga agcaacaaat
     3661 tcactcgatg caggaagtga aaaggtaata gttgaaaatc ttaaaagctt tttcaaaaac
     3721 aagactgtaa taattgttgc tcacaggcta agcacggtaa gggatgcaga taagattgta
     3781 gtactcgata acggacgaat tgttgagtca ggtacacaca atgaacttat tggaaggcag
     3841 ggggcttact ttaatcttat aaaaaatcag cttgaactcg gtaactgaat aatactctga
     3901 taaagcgcgt aagggtgaaa aagcacgaaa ttcaatattc tgagcctgtg agggagataa
     3961 tgggaaatcc tccgggtaaa atcctcagat ggggcaccat gattatattc gcaatatttg
     4021 ttttattcat ttcttttgca tggctgatta aatatcctga tattgttcct gctcaggttg
     4081 aaataacaac cgaaaaccct cctgcttcca taatgtctaa aacaacaggc cgcataatgc
     4141 acctgatggt ttcggataaa cagcaggtga aggctggtga aatgcttgca gtaatggaaa
     4201 acccggcatc atttgaagag gttcgaaaac taaaaaactt tattgataca tttaacctca
     4261 ggagtgatgt aaaaattgaa actattccag atttttacaa ccttggagaa ctgcaaactc
     4321 cctatgaaac attcagaaag agtgtatcag atattgataa ttttattaag aacgactact
     4381 ataatgccag aataaatgct gtgaatgaag aactgaggga gctgaaaaca tatactgcac
     4441 gtttacagga aaatgaaaaa ttctataatg aaaattatct tattgaactt aataaattca
     4501 ggcgcgattc agcattaaat gcaggtaaat caattgctcc ggcagattat gaaaactcaa
     4561 aacaggcatt aatcagacaa caaattgaat tacagaaagt acggctggat atttcagcaa
     4621 agaatatcga aatgattaat aaaaggcaac ttatagaaga atattcaata aaacgttcag
     4681 aagagcttga taagctttat tctaatgctc gtgaagcatt gaagaacttg agggcggagc
     4741 ttagcatctg ggaaaataca tatttgctcg tgtctccagt ggaaggacag gttacttttt
     4801 caaggtactg gtcagtaaat cagcttgtga agaaagatga aacagttatg tcagttgtgc
     4861 ctgataatcc cgggaactat attggtaaaa tctatcttaa aatgtataaa tcaggtaagg
     4921 tacgggaagg gcaaatagtg aacataaaac taaacggata tccttatatg gaatatggta
     4981 tgctacaggg tgttatacgc tcaaaagcac ttgttccagc tggtgattca tatctgattg
     5041 aagtggaact taaaaacggg cttacaacat tatataaccg gactcttgag tttacccaga
     5101 atatgacagg agtggcagag ataattactg acgacagaag ccttctggag aaaatgattt
     5161 atccattcag gtatcttatt tcccgtaaca agagataaga aaaccttatt tatttttata
     5221 aggttcatat tcttcttcct gcggggaaga acttaaatcc tgcttgcttt ttgccttatt
     5281 tgcacctgat gtacccttct ccttgtaacg aagataaagc cttattgcaa ctataataaa
     5341 aactgtaaca gtaatgaaaa ttgagaccgg atctttcatt gagatatttt aattttcagc
     5401 gttcaaagat atcaataagt tacgaattta cgacaggatt ttttaaacag ctttatattc
     5461 gataaacaag tagcttcata ttaaaatttc attatttgaa ttaaaacttt gagttttatt
     5521 aaaatattca atattttcgt gtctcaaaaa tttcatgggt ttccatgtaa tttgctagtg
     5581 aataaaatac ttgaaagggg ttgaaaatga ttgagaaaga tccaaacaat cctgttctga
     5641 atgaacaatt cgcagctact gaaggtgctg gcgaaagtac agggaatgtg cccggaggta
     5701 atattgaacc tcctgttgat ttttctgtta atgaaacgga aaaaccggaa agtctgacgt
     5761 ctgatgagaa gaaaaaccgg gatggggaag tggacatgac agaattttct gttccggaaa
     5821 aagaaaatga ggaaaaaatc aacaccggaa ctgaaagtgt gccagttgtt cagtcaaccg
     5881 aaacagctga agccagtgag gacttatctg actcgggtga acatatatca ggcggggaaa
     5941 aagttaaaag gccaagaaag aaaagcggag aaaaaaaatc cgtttcacca gtcattattc
     6001 ctgaaacaag cctggcagct gagaggcagg gggatatacc tgaagattat aaggatattg
     6061 aactacctcc tgttgattat tcgggttatt caaaagaaaa actggttgaa acacttgaat
     6121 tgattattga caacagacct ccaaatgaga taaaaaatga tgttgaaagg ataaagagtc
     6181 tgttttacaa gaagataaga aaagagaacg aggaaaggaa aaagaagttt ctcgaagagg
     6241 gaggtaaggc tgaagattac agagttcttg ttgaccctct tgaggttaaa gtgaaggagc
     6301 tacttgaaaa atacaaatcg aaaaagagtg atttcgggaa aattcaggag gctgaaaaac
     6361 ttgagaatct cagaaagaaa tatgagataa ttgagaaaat taaggatctg gttaacaggg
     6421 aagaatcaat aaataaaaca tttcaggaat tcagagctct tcagaacgaa tggcatgcag
     6481 tagggattgt tcctcaaagt gcattgaaag acctgtggga gaattatcat catgcagttg
     6541 agatatttta tgattatata aaaatcaaca aggaactccg ggatcttgac cttaagaaaa
     6601 atcttgaggc aaaagtaaga ttatgcgaaa aagctgaaga attaatgctt gaacctaatc
     6661 cggttacagc atttaaattg ttacaggatc ttcacaatgc atggagggag attggtcctg
     6721 taccacatga gtcgaaaaat gagatttggg aaaggtttaa ggaggctact gccaaaataa
     6781 ataaaaggca tcatgagttt tttgaaaaac agaaggagga gcagaagaaa aaccttgaag
     6841 ccaaaatcgc cctttgtgaa aaagtagagg agattaacag ggaagagctg aaaactttta
     6901 aagattttga agaaaaagcg aagattgtta tagatcttca gaagatgtgg cgaaccatag
     6961 gctttgctcc gaaaaagcaa aacaataaaa tataccagcg tttcagggaa gcatgtgatg
     7021 ctttttttga aaagaaaagg aacttttatg ccgacagtaa agagatgcag atgaataacc
     7081 tccagcgtaa gacggatctc tgcattcagg ctgaggcatt gcaggaaagt accgactgga
     7141 aagccacgac cgaagcactt atcaggcttc agaaagagtg gaaagaaatt ggtcctgttc
     7201 caagaaaata ttcagataaa atatggaaaa gattcaggaa ggcctgtgat catttcttca
     7261 acagaaaggc tgaatatttc gccagtctcg attcaacata tgaagaaaac ctgaaagcta
     7321 aggaggctat tattgccgaa cttgaaaatt tcgagccggg tgatgatgta caggaagcct
     7381 tcaataaact taaagaaatc cagaaaagat ggaccgatat tggttttgtg ccttttaatc
     7441 gtaaggagga aattaataac aggtatagaa atgctcttaa caaacaattt gacaaattga
     7501 agattgacga agttgacaag agcatcctga aatacaaaac aaaacttgaa acccttaagt
     7561 caaatccgaa ggcactgcgt aaactcagca atgaaaggga aaagtttata tcaaaaatca
     7621 aacaacttga aaatgatatt gttctgtggg agaacaacat tggcttcttt gcaaaatcgc
     7681 ccaacgcaga aacaatgatc aaagaagtcg aggaaaaaat aaagaatgca aagaaaacga
     7741 tcaaaacgct tgaggaaaaa gttaagctta ttgaccgtat taatgttgag gaattatagg
     7801 gaataaaatt tagtttgttt tggctgacac aaagtatgta tttgttacag gtggagttac
     7861 atcgtcactg gggaaaggta taatatctgc ttctctggca aagctgctcc agtcgagagg
     7921 gtattctgta actattcaga aactggatcc atatatcaat gtagatccgg gaacgctcaa
     7981 tccatacgag cacggagaat gctatgttac tattgatggt gctgaaactg atctagacct
     8041 tggtcattat gaaaggtttc tcaatgttac taccagtcag gcaaataacg tgacaaccgg
     8101 aaaagtttac cagacagtta ttgaaaaaga gcgcaggggt gactatctgg gtaagactgt
     8161 acaggtaata ccacatatta ctgatgaaat aaaaagacgt attaagctat tggcctcaag
     8221 caaaaaattt gatattgtaa ttactgaaat tggaggtacc gtaggtgata tcgagtcgct
     8281 accctatatt gaatccgtga ggcaactacg gtgggaaatg ggtcagggaa actgtgttgt
     8341 tatccacctt acacttgtgc catacctttc tgctactgga gaacagaaaa caaaaccaac
     8401 tcagcattca gtgaaagcct tgcttgaggc tggaattcag cctgatgtcc ttgttttgag
     8461 aacggaaaaa tccatctcca gtgatatcag aaaaaaagtg gcgcttttct gtaatgttga
     8521 agaaaaagca gttattgagt cagtcaatgt aagcacaatt tatgaagtcc cactgagaat
     8581 gcttgaagaa ggactggatc aggttgtact tcagaagctt aatttgccgg tgaagaataa
     8641 acccgatatg tcagaatggg aaacttttgt tcataaactt aattttccga aaagaaaagt
     8701 aaatattgcg cttgtaggga aatacgttga gcttcacgac tcatacaaat cgatacatga
     8761 atcatttata catggaggtg cagccaacga ttgttccgtc aatattggtt attttaattc
     8821 ggagaatatc tctgacacga acgtagctga actcatgaat gatattgatg gaatactaat
     8881 tgcaccagga tttggctcca gaggagtgga aggtaaaatc agtactgtaa aatatgcgag
     8941 gattaataat gtgccatttt tcggcatttg cctcggtatg cagtgtgcag taattgaatt
     9001 tgccagaaat gttattggtt atgaggatgc acattctacc gaaatggatt ccgataccaa
     9061 acaccctgta atagatctga tggaagagca gaaaagcata acagaaaaag gtggtacaat
     9121 gagattgggt ggttatagat gtatactttc acctgattcc ctgtcatata aagcatatgg
     9181 caaaaaagaa ataattgagc gtcaccggca ccgatatgag tttaacaaca cgtatcttga
     9241 ggagtttaaa caacatggaa tgcttatggc cggaataaac cctgaatctg atcttgtgga
     9301 aataattgaa ttaccatctc acagatggtt tgtgggggtt caatttcacc ctgaatatag
     9361 tagtactgtc atgaatcccc atcctttatt tgtaagcttc ataagggcat gtatcgaatt
     9421 tgctgacttt aaaagaggat aaaaaatgga taagaatacc ataacaggtc tgatactcat
     9481 cttcttaatc tttatcggat tcagtattta taataacgca aggcttaata aaatttataa
     9541 tgaaaaa
//
LOCUS       DAOSNB010000063         9541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7133_length_9541_cov_4.458781, whole genome shotgun sequence.
ACCESSION   DAOSNB010000063 DAOSNB010000000
VERSION     DAOSNB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_9541_cov_4.458781"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..925)
                     /locus_tag="PLG42_04110"
     CDS             complement(170..925)
                     /locus_tag="PLG42_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU30429.1"
                     /translation="MDKIFEILINRRTIRKYSEKDVDDALLNKILAAGIRASTTGNMQ
                     VYSIIVTRDDEIKKQLLPLHFNQKMVTEAPVLLTFCADFNRFNKWCRMRKAEPGYDNF
                     LSFLTAAIDALLVAQTVCIAAESSGLGICYLGTTTYMAHKIIEVLKLPEGVVPVTTIT
                     LGWPAEIPEQVDRLPLDAVIHHETYHDYSAEDIDRIYHDKEQRTDSLQYIKENNKETL
                     AQVFTDVRYKKEDNVYFSRMLLKVLEEQGFMNQ"
     gene            complement(1122..2072)
                     /locus_tag="PLG42_04115"
     CDS             complement(1122..2072)
                     /locus_tag="PLG42_04115"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOU30430.1"
                     /translation="MKSMFKMFIQINKILLLLILVIMSSENFAQKQPPLNELLPAESA
                     VILGKGTEPPFSGKYEKLFATGTYICKQCGSALFYSDSKFDAHCGWPSFDAEIPGAVT
                     RKPDPDGRRTEITCSYCGGHLGHVFTGEGFTPRNTRHCVNSLSLDFVPTKLEPGRYGT
                     AIFAGGCFWGVEYFLQKEPGVVSVTSGYTGGHVKNPSYREVCTGKTGHAEAVKVIYDT
                     RKTSYEKLLRLFLEIHDPTQVNRQGPDVGEQYRSEIFWLNSEQKTLAEKYIGILKSKG
                     LNVATRITKASEFYEAEDYHQDYYFKTGKLPYCHGYTKRF"
     gene            2265..2393
                     /locus_tag="PLG42_04120"
     CDS             2265..2393
                     /locus_tag="PLG42_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30431.1"
                     /translation="MKRLVFILLLATVIVVATSSCTKKTCPAYSKAVTENTELRRG"
     gene            2435..3229
                     /locus_tag="PLG42_04125"
     CDS             2435..3229
                     /locus_tag="PLG42_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30432.1"
                     /translation="MRKLYISALFVLFFGFQLSGQGEVEEQPRIVFRNEFTFGLILST
                     DGYGISYREGKRLDYFNRRLFEFDMVTLRHPKEVRLSNPAYQTPGTFIFGKKNSVIIL
                     RGGIGHQKEIFEKGDLGGVAVRYFYTAGPVIAFYKPIYYKILKFVSSYEAEIIEEKFD
                     VRKHDPTMIYGKCSFFRGIEEGKFLPGLYGKAGFNFEYSRQEKVIHAIEIGAQIDAFP
                     KAIPIMDVAKNKSVYFSLFASYRFGMIIDPLNPEANKLSNIFRVRR"
     gene            3318..4322
                     /gene="gap"
                     /locus_tag="PLG42_04130"
     CDS             3318..4322
                     /gene="gap"
                     /locus_tag="PLG42_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOU30433.1"
                     /translation="MSKIKVAINGFGRIGRNVFKIALERNNIEIVGINDVTDTKTLAH
                     LLKYDSTQGKFEGVTCDDENIIVHGKKYRVTAEKNPAVIPWVEKPDVVIESTGVFTSK
                     ESPKGGYGDHLKNGAKKVILTVPAKDEIDAMIIIGVNEKDLKPEHKCISNASCTTNCL
                     APVVKVLNDNFGIEMGYMTTIHSYTNDQRILDLPHKDLRRARAAALSQIPTTTGAAKS
                     IGKIIPELKGKLDGVAVRVPTPTGSLVDLVAVLKREVTKDEINAAMKKAAEGPMKGIL
                     EYTEDPIVSCDVIHNPASSIFDALSTMVNGKMVKVFSWYDNEWGYSCRVVDLIPLVMK
                     "
     gene            complement(4519..4896)
                     /locus_tag="PLG42_04135"
     CDS             complement(4519..4896)
                     /locus_tag="PLG42_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU30434.1"
                     /translation="MEKLVIQEELKNCPRIYYYPAINKLELKGRSIPENPELIFRKLD
                     EWITSHFEKNDSLDVDIMLEYINSGSSKYLYEILRKLSFFIDSGKKINIKWLYEEDDE
                     AMLELGEHYRDAAGIPLTIEMII"
     gene            complement(4914..6158)
                     /locus_tag="PLG42_04140"
     CDS             complement(4914..6158)
                     /locus_tag="PLG42_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU30435.1"
                     /translation="MFRFSIFRENLRIAVRAIKSNRVRTILTVCIIAFGIMALIGILT
                     AIDAIKTSLTEQFTMMGANSFTISSRGMNIQIGSSSYRARNYSRISYREAEEFKRRFD
                     EPAWVSISYNASGMATVKYGSEKTNPNVSLVGVDEDYLTVSGYEIESGRNFSQEEVRM
                     ARHYVVIGSEIVRSLYPAGTDPVGTEINVAGMNLRVIGVLKSRGASALSSDNVCLMPV
                     TTARQYFSRPNMTFSITVMPHKPANLEVVAGAAEAVFRIVRNLNPRDESDFNITRSDN
                     IVTLLLQNIRYVTLAATLIGIITLFGASVGLMNIMLVSVTERTREIGTRKAVGAKPST
                     IKNQFLFESILIGQMGGFFGIILGVIIGNVVSSMLKSSFVIPWFWVGTGIVVCFIVGI
                     ASGYAPAVKAANVDPIEALRYE"
     gene            6323..6706
                     /locus_tag="PLG42_04145"
     CDS             6323..6706
                     /locus_tag="PLG42_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOU30436.1"
                     /translation="MRLTRAEEEVMQVLWKIKKGFVKDILEHFPEPKPAYTTVSTIIR
                     ILQDKGYVTHKAHGRSHEYFPQISKEEYTKTHIKNFVHDYFSRSFKNLISFLIESKSI
                     SLKEMEESIKIMQNEVKRRKKKQRK"
     gene            6843..7883
                     /locus_tag="PLG42_04150"
     CDS             6843..7883
                     /locus_tag="PLG42_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOU30437.1"
                     /translation="MKLSDRISCFAQLGDILREALEGRGKYAGKIENLISRQQFVNPW
                     FTPENVRMALNAISANLTEEKLKEWTGRYPELNMNYSPVNVGVIMAGNIPLVGFHDFL
                     SVLITGNRVIARTSSKDPELPVAIAEILYDISDEFRELIEFSDGIIKGFDAVIATGSN
                     NTSRYFEYYFGKYHSLIRKNRNSVAIITGNEDESELEKLGEDIFSYFGLGCRNVSKIF
                     IPEYFDVDSLKKYWEKYSGLINHTKYGNNYDYYKAVYLVNKEAFTDSGFYLMKEDPAY
                     ASPVSVIYYEYYRRDEDFSFLFDSDKEKIQCIVGRGLIDFGKTQWPELWDYADDIDTI
                     EFILKKNLGEAL"
     gene            7929..8480
                     /locus_tag="PLG42_04155"
     CDS             7929..8480
                     /locus_tag="PLG42_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30438.1"
                     /translation="MVYKFVVLSDEDDSFVREFELLDRQTLLDFHNILQEELEFDKSQ
                     MASFYLASNNWEKEEEFTLIDMGAGSSTMEDAVLEEIIYKKNQKLLYVFDFFNERALF
                     IEYVGDTKEVEGRQYPVCTNSKGLPPKQIIFGTASRRNLSPLAIDDEEEEEDIDVDDV
                     FFNGDEDEGLGDFESFDQAEEEE"
     gene            8505..9428
                     /gene="miaA"
                     /locus_tag="PLG42_04160"
     CDS             8505..9428
                     /gene="miaA"
                     /locus_tag="PLG42_04160"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOU30439.1"
                     /translation="MQNTLIVVVGPTGVGKTELSIDLAGHFKTEIISCDSRQFYREMV
                     IGTSAPTEEQLSKVRHHFVRFLSVTDYYSASMYERDVLKLLPELFSRNPLVILTGGSG
                     LYVDAVCIGIDDIPDVDPAAREKYWNLYQRDGIESLRIALKLLDPDYYRIVDLKNHKR
                     IIRALEICETTGRPYSSFLKKEKRQRDFRIIKIGLERPRDELYTRINERVDRMIGEGL
                     VEEAHNLLKYRHLNALQTLGYKELFEYFDGKITLETAISLIKRNSRRYAKRQITWWAR
                     DKEIRWFNPLQKDDIIMYIETEVLRQNQCTG"
BASE COUNT         2942 a         1895 c         1937 g         2767 t
ORIGIN      
        1 ccgtggtgtc gaccctctta ctgctgaaga cattgctgat gtaatctact ggtgtgcaac
       61 ccggcccccg catgtttgca taaacgatat caccataaca cctctgcaac aggctaatgt
      121 gcaccatatc aacagaagac aatagggagc cttctggaca agccagatgt cattgattca
      181 taaatccttg ttcttcaaga actttgagta acattctgga aaaatagaca ttgtcttcct
      241 ttttatatct cacatctgta aagacctgtg ccagggtttc cttgttattt tcttttatgt
      301 attgcaatga atcggttctt tgttctttat cgtgatagat tctatctata tcctctgcag
      361 aataatcgtg gtaggtttca tgatggatta ctgcatcaag aggcagacgg tcgacctgct
      421 ctggaatctc tgcaggccaa cccagtgtta ttgttgtaac aggcaccact ccttcgggca
      481 gtttaagaac ctcaataatc ttatgtgcca tatatgttgt agttccaagg tagcatattc
      541 ccagacccga agactcagct gctatgcaaa cagtctgggc cacaagaaga gcatcaatag
      601 ctgctgtgag gaatgaaaga aaattatcat agcctggctc tgcctttctc attcggcacc
      661 atttattgaa tctgttgaag tcggcgcaga aagtgagaag caccggtgcc tctgtaacca
      721 ttttctggtt gaaatgcaaa ggcagaagtt gctttttgat ttcatcatcg cgagtaacta
      781 ttatgctgta aacctgcata ttacctgttg tggatgcccg tatacctgca gcaagaattt
      841 tattcagtaa tgcatcatct acatccttct cagaatattt cctgatggtc cttctattta
      901 ttagaatttc aaatatctta tccatgcttc attctttttt gtttgactgt aaaatattcc
      961 ctgttaattg aaaatgtgtg taaagataag aagtaaataa ttacgcaaat gccgattacc
     1021 gattaccggt taccctttta cctctagccg gttcctccct gagcatgaag gaagcaggat
     1081 aaccttatgt tactggctct tcaggattcg ttactggcaa cttagaaccg ttttgtatag
     1141 ccgtggcaat aaggcagttt acctgtttta aaataataat cctggtgata atcttcggct
     1201 tcgtagaatt ctgatgcttt tgtgatgcgg gttgcaacat tcagcccttt gcttttcagt
     1261 atacctatat atttctcggc cagtgtcttt tgttcactgt tcagccagaa tatttctgag
     1321 cggtattgtt ctcccacatc cggtccctgc ctgttaacct gggtggggtc gtgtatttca
     1381 aggaaaagtc tcagaagctt ttcgtatgat gttttgcggg tatcgtaaat taccttcacg
     1441 gcctctgcgt gtccggtctt tccggtgcat acttcccggt atgaaggatt tttaacatgt
     1501 cctccggtat atcctgatgt aacagacaca acaccaggtt ctttctgaag gaaatattct
     1561 actccccaga aacacccacc tgcgaatatt gctgtaccat accttcctgg ctcaagtttt
     1621 gtaggaacaa aatcgagtga aagggagttc acacaatgac gggtgttgcg cggagtaaat
     1681 ccctcacccg taaatacatg accaaggtgc cccccgcaat agctgcaggt aatttcagtt
     1741 cgcctgccat cggggtcagg cttgcgcgta actgccccgg gtatttcggc atcgaagctc
     1801 ggccagccgc aatgtgcgtc aaacttgctg tcggagtaaa acaacgccga cccgcattgt
     1861 ttgcaaatat atgtaccagt tgcaaagagt ttctcgtatt ttcccgaaaa aggaggttct
     1921 gttccttttc caagtatcac agccgactct gcaggcaaca attcgttcaa aggaggctgt
     1981 ttctgggcaa aattctcaga gctcataatg acaagaatca gtaatagtaa aattttattt
     2041 atctgaataa acattttaaa catgcttttc atctaacaac aatttcctgt gcaaatggtt
     2101 caccagaaac acaataaaaa taagcatgat gcgtttggtt attaccctga actgaaaata
     2161 aaaaacagat tttgaacatt caaatattaa aattgttata acagtacttg tttttaacat
     2221 taaaaactta tttttgtcat agaaatttta accagaatta taacatgaaa agacttgttt
     2281 ttattctgct tctggctact gtgattgttg ttgcaacaag ttcgtgcaca aaaaaaacat
     2341 gtcctgccta tagcaaagca gtgactgaga atacagagct gagaaggggt tgaaacaccc
     2401 tcttattata atttcacctt tcgggaaaac tgtaatgaga aagctgtaca tatcagcatt
     2461 gtttgtgttg tttttcggat ttcaactttc cggtcagggt gaagttgaag aacagccaag
     2521 gattgtcttc cgcaatgagt ttacatttgg tttgatttta agcaccgacg gttacggaat
     2581 aagttaccgt gagggtaaga ggctggatta tttcaacaga aggcttttcg agttcgatat
     2641 ggttaccctg cggcatccca aagaggtcag gctctcaaat ccagcctatc agacacccgg
     2701 aacgttcatt tttggcaaga aaaactccgt aattattctg aggggtggta ttggacatca
     2761 gaaagagatc tttgaaaagg gagatcttgg aggagtagct gtgaggtatt tttacactgc
     2821 ggggcccgtt attgcctttt acaagcctat ttattacaaa attctcaagt ttgtaagttc
     2881 ttacgaggct gagatcattg aagaaaaatt tgatgtcagg aagcatgatc ccacgatgat
     2941 ttatgggaaa tgttcttttt tcagaggcat agaagaaggc aagttcctac cgggtcttta
     3001 cggcaaagca ggtttcaact ttgaatacag caggcaggaa aaagtaattc atgccattga
     3061 aatcggagcc cagatagatg catttcccaa agctattcca ataatggatg tggctaaaaa
     3121 caaatctgta tatttctccc tctttgccag ttaccgtttc gggatgataa tagatcctct
     3181 gaatcctgaa gcaaacaaat tgtcaaatat tttcagggta agacggtagt aattcaattt
     3241 atcacatacg atttgccaga taaatttttt tttctatttt tggcagttca ttgtattaac
     3301 cattaaaatt tattgatatg tcgaaaatta aagttgcaat taatggattc ggaagaatag
     3361 ggcgcaatgt ttttaaaatc gctcttgaaa gaaataatat tgaaatagta ggtatcaatg
     3421 atgtaacaga taccaaaact ctggctcatt tgttgaagta cgactcaaca cagggcaaat
     3481 ttgaaggagt cacctgtgat gatgaaaata ttattgttca cggcaagaaa tacagggtga
     3541 cggctgaaaa aaatcctgct gttattccat gggttgagaa accggatgtt gtaattgaat
     3601 cgactggtgt tttcacatca aaggagagtc ccaaaggtgg ttatggtgac catctgaaga
     3661 atggcgcaaa gaaggtcatt cttactgtac ctgcaaagga tgaaatagat gcgatgatca
     3721 ttatcggtgt caatgagaag gatcttaaac ccgagcataa gtgtatatca aacgcatcgt
     3781 gcactaccaa ctgtcttgcc cctgttgtta aggttctgaa cgacaatttc ggaatcgaga
     3841 tggggtatat gaccacgata cattcatata caaatgatca gaggattctt gatcttccgc
     3901 acaaagatct gaggcgtgcc cgcgctgcag ccttatcaca gattcccacc accacaggag
     3961 cagcaaaatc aataggcaaa attatacctg aactcaaggg caaactcgac ggtgtggcag
     4021 taagagtacc caccccgaca ggttctctgg ttgacctggt tgctgttctc aagagagaag
     4081 ttacgaagga cgagatcaat gctgcaatga aaaaggcagc tgaaggaccg atgaaaggca
     4141 ttctcgaata tacagaagat cctattgtat catgtgatgt tatccataac cctgcctcat
     4201 caatatttga tgcactcagc acaatggtaa acggcaagat ggttaaggtg ttctcatggt
     4261 acgacaacga gtggggttat tcgtgccgcg ttgtggatct cattccactt gtaatgaaat
     4321 agcaggcacg ggaataaatc ttgttttcat gaaaaagggg cttctgaaaa agcccctttt
     4381 tattttgtat caaacagtta ttgttttttc ctgcaagtct ctaagatctt aaatccagat
     4441 tctcaacctg gaaattttca cttatcgctt ctttatcata aaagctgact gaaaggagac
     4501 cctaacctta acgactattc aaattatcat ttcaattgtg aggggtattc ctgcagcatc
     4561 gcgataatgt tcaccgagct ccagcatagc ctcatcatct tcttcgtaca gccatttgat
     4621 gttaattttc ttcccggaat cgataaagaa tgaaagtttc cgaagaattt catagaggta
     4681 tttcgaagat ccgctattga tgtattccag cataatatca acatcgagcg aatcattttt
     4741 ttcaaaatgg ctggtaatcc actcgtcaag tttcctgaaa ataagttcag gattttccgg
     4801 aatagaacgg ccctttaatt ccagtttatt tattgcagga taataatata ttctcggaca
     4861 gttcttaagt tcttcctgaa tgactaattt ttccatatag aaaaatattt taattattca
     4921 tatctgagag cttcaatcgg gtctacatta gctgccttca cagctggcgc gtaacccgat
     4981 gcaatcccaa caataaaaca aaccacaatt cctgttccaa cccagaacca tggaataaca
     5041 aaactggatt tcagcattga agatacaaca tttccgataa ttacacccag aattatgcca
     5101 aaaaatccac ccatctgtcc tatcaatatt gattcgaaga ggaactggtt tttaattgtt
     5161 gaaggttttg ctcctactgc cttccgggta cctatctcgc gtgtacgttc ggtaaccgag
     5221 acaagcataa tattcataag tccgacagaa gcaccaaaga gggttattat accaataagt
     5281 gttgctgcca gagtaacata cctgatgttc tgaagaagca gtgtaactat gttgtcgctt
     5341 cttgtaatat taaagtctga ttcatccctc ggattaagat tgcgtacaat tctgaatacg
     5401 gcttctgcag cacctgcaac aacttcgagg ttggcaggtt tgtgaggcat cacggtaata
     5461 ctgaatgtca tattgggcct cgaaaagtat tgccttgccg ttgtgactgg catcaggcac
     5521 acattatcac tacttagtgc actggcaccc ctcgacttca gtacacctat aaccctcagg
     5581 ttcatgccgg caacatttat ctccgttccc accgggtcgg ttcctgccgg ataaagactc
     5641 ctgactattt cagaccctat cacaacataa tgccgtgcca tcctgacttc ttcctgcgaa
     5701 aagtttctgc ccgattcaat ttcataaccc gaaacagtca ggtagtcttc gtcaacaccc
     5761 acaagcgaca cattcgggtt agtcttctcc gatccatatt ttacagttgc catccccgaa
     5821 gcattataag aaattgacac ccaggcaggt tcatcaaatc ttcttttgaa ctcttctgct
     5881 tcccggtatg aaatccttga atagttcctc gccctgtaac tcgaacttcc tatctgaatg
     5941 ttcatccctc tcgatgatat cgtgaatgaa ttagccccca tcattgtgaa ctgctcagtg
     6001 agagaggttt taatggcatc aatggcggta agtataccaa taagagccat aataccgaat
     6061 gcaataatgc agacagttaa gatagttctt acccggtttg actttattgc tcgcaccgca
     6121 atccttaaat tctccctgaa aatactgaat ctgaacataa taccgggttt agctaattga
     6181 ccaacagatt tagagctaaa aatacaaaat tttcaactag atacaaaaag ctgttcaact
     6241 aaaaaatatt taacaaatta gttgaaaaac taaaaaattc gttatatttt tgtattggta
     6301 aataattaaa aaatttaaaa tcatgagatt aacaagagca gaagaagaag ttatgcaggt
     6361 actctggaaa attaaaaagg gatttgttaa agatatcctg gaacatttcc cggaacctaa
     6421 acctgcctat actacagtca gcactataat aagaatcctt caggacaaag gatatgtgac
     6481 ccacaaggca catggccgct cacatgaata cttccctcag atatcaaaag aggaatatac
     6541 aaaaactcat attaaaaatt ttgtacatga ctacttctcc agatctttca aaaaccttat
     6601 aagttttctt attgaatcaa aaagcatatc attaaaagag atggaggaaa gcattaaaat
     6661 catgcagaat gaggttaaaa gacgcaagaa aaaacaaagg aaataaactt ataaaatgct
     6721 gcagtaaatc aattttttta cctgaaaaaa caaaaagcct tcccgttttt taaagcagga
     6781 aggtttttgt ttttcatact cactggaaaa aactaatttt gcatcgttac agaattcaca
     6841 aaatgaaatt atcagacaga atttcatgtt ttgcacagct gggggatata ctcagggagg
     6901 cactggaagg caggggaaag tatgccggaa aaattgagaa tcttatcagc cgccagcagt
     6961 tcgtaaatcc atggtttaca ccggaaaacg tcagaatggc tcttaatgcc atttctgcca
     7021 atctaaccga agaaaaactg aaggagtgga ccggaagata tccggagcta aatatgaact
     7081 attcacctgt taatgtggga gttatcatgg caggtaatat tccactggtg ggctttcacg
     7141 atttcctatc ggtactcatc acaggcaaca gagttattgc acgcacaagt tctaaggatc
     7201 ccgagctgcc ggttgctata gccgaaatac tatacgatat tagcgatgaa ttcagggaac
     7261 tgatcgaatt cagtgatggt attattaaag gattcgatgc tgtgattgca accggaagca
     7321 ataacacatc gagatatttt gaatattatt tcgggaaata tcacagtctc ataagaaaaa
     7381 accgtaatag tgttgccatt attacaggca atgaggatga aagtgagctc gaaaagcttg
     7441 gtgaggatat tttttcatat ttcggacttg gctgcagaaa cgtttcaaag atttttattc
     7501 ctgaatattt tgatgttgat tctttaaaaa aatactggga aaaatattcc ggactcatta
     7561 accacacaaa atacggtaac aattatgatt attacaaagc tgtatatctt gtaaacaaag
     7621 aggctttcac cgacagcggt ttctatctga tgaaagaaga cccggcctat gcatcacctg
     7681 tctctgtaat ttattatgaa tattatagaa gagatgaaga tttttctttt cttttcgact
     7741 ctgacaaaga gaaaatacag tgcattgtag gaaggggtct gattgatttt ggaaaaactc
     7801 aatggcctga attgtgggat tatgctgatg acattgatac aattgaattt attttaaaaa
     7861 aaaatctggg agaggcattg taaaaacaaa aaaatattat attgcaccgc ctcaattagc
     7921 aaagagttat ggtttataaa tttgttgtac tatcggacga agatgactca tttgtaaggg
     7981 agtttgagtt gctcgatcgc cagactctgc tcgattttca taatattctt caggaagaac
     8041 tggagtttga caaatcgcag atggcttctt tttatctggc atcgaacaac tgggaaaaag
     8101 aagaagaatt caccctcatt gacatgggcg caggttcttc taccatggaa gatgcagttc
     8161 ttgaagagat aatttataaa aagaatcaga aattgcttta cgtcttcgat ttctttaatg
     8221 aaagggcgct tttcatcgaa tatgtaggtg atacaaagga agttgaaggc aggcagtatc
     8281 ctgtttgcac caattccaaa ggcttaccgc ccaaacaaat catcttcggc actgcttcaa
     8341 ggagaaacct gagtcctctt gccattgatg acgaggaaga agaggaagac attgacgtgg
     8401 atgatgtatt tttcaacggc gacgaggatg aaggactggg tgatttcgaa agcttcgacc
     8461 aggcagagga agaggaatag acctcttcaa ttccttgata cttcatgcaa aacactctta
     8521 ttgtggtcgt tggcccgaca ggtgtcggga aaacagaact ctcaattgac ctggccggac
     8581 atttcaaaac tgaaatcatc tcctgtgatt cccggcagtt ttacagagag atggttattg
     8641 gcacgtcagc gcctaccgaa gagcagctga gcaaagtaag gcatcatttt gtacgattcc
     8701 tttccgtaac agattactat agcgccagca tgtacgaaag ggatgtgctt aagctgcttc
     8761 cggagctttt ttcccggaat cctctggtta tcctgactgg cggctcaggc ttatacgtcg
     8821 atgccgtatg tattggcata gacgatatac ctgatgttga ccctgccgca agggaaaagt
     8881 actggaacct ttaccagcgg gatggtattg aaagcttgag aatagcactc aagttactgg
     8941 atcctgatta ttacaggata gttgacctga aaaaccacaa aaggattata agggcactgg
     9001 aaatatgtga gaccacagga aggccatatt cttcattttt aaaaaaggaa aagcggcaaa
     9061 gagatttcag aattataaaa atagggttgg aacggccacg tgacgagctc tacaccagga
     9121 tcaatgaaag ggttgacaga atgatcggag aaggacttgt ggaagaagca cataatcttt
     9181 tgaaatacag acatctgaat gcccttcaaa cactcggcta taaagaactg ttcgaatatt
     9241 ttgacggaaa aataacatta gaaactgcaa tcagtctgat taagcgcaac agccggaggt
     9301 acgcaaaaag gcaaattaca tggtgggcaa gggataaaga aatcagatgg ttcaatcctt
     9361 tacagaaaga tgatataata atgtatattg aaacagaagt cctcaggcaa aatcaatgta
     9421 caggctgaag atttttaagc ttataaatgg ttccgaccgg gtcgtctgaa ctgaaaactg
     9481 tatttcctgc caccagaaca tttacacctt tccctacaag ttttgcagca tttgaaaggt
     9541 c
//
LOCUS       DAOSNB010000064         9534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7142_length_9534_cov_4.436966, whole genome shotgun sequence.
ACCESSION   DAOSNB010000064 DAOSNB010000000
VERSION     DAOSNB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_9534_cov_4.436966"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLG42_04165"
     CDS             complement(<1..1005)
                     /locus_tag="PLG42_04165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOU30440.1"
                     /translation="MKKIFILFQALLILAAYSCTNPVTKTSPAPVLKEATPESAGMSA
                     ERLSRIDKMIQSAVDSGWIAGAVGFISRDGKIIYHKAFGVSDLEAKTQLKKDDIFRIA
                     SQTKAITSIAAMMLFEEGKFLLDDPVSKYIPEFKNPRVIEKFNEKDTTFTTVPAKREI
                     TIRDLFTHTSGIDYAGIGSPTMRAIYAKYDIPGGFGTDKMVIGDKMRALGKLPLAHNP
                     GERFTYGLNVDVLGYLIEIWSGESLDEYFRNHIFVPLGMNDTYFYLPEEKHNRLVKVN
                     TEDENHHVRAMPQDAVNYPLLKGTYYSGGAGLSSTVYDYAVFLQMLLNRGEYNGKRLL
                     S"
     gene            complement(1093..2955)
                     /locus_tag="PLG42_04170"
     CDS             complement(1093..2955)
                     /locus_tag="PLG42_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU30441.1"
                     /translation="MKKISLTLFITLFSLISLKSFTQPVVALDNWFNRETNPKTGQLY
                     HYLWTDTAWSGYSRWGEIFKGKGAVITTIEKPAADVLKNINVYIIVDPDTTTESKTPN
                     YITQEDAKTIASWVKKGGVLAVLANDAPNCEFTNLNRMMSQFGITFNHVTLHPVPGTN
                     WEMGACTNLPNHPLFKDVSKIYIKEVSSISLTRNARPVLVENGNVLIAECNYGKGYVF
                     AIGDPWIYNEYIDHDRLTPDFENRKAAENLAGLLLGKVKKYPGAPGTFKPLPKEKTLQ
                     AMVLANKYFMEKWPDVGKTIITDRERPSNIWTRGTYYEGLMALYKIKPDPSYLDYAIK
                     WGEFHKWGLRNGITTRNGDDQCCGQTYIDLYLMDTTKKERIRDIKACIDNMISTDKID
                     DWSWIDLIHMAMPVFARLGAVYNDNRYFDRLFEMYMFTKTKHGDNGLYSTKDHLWWRD
                     KDFDPPYKEPNGRNCYWSRGNGWVLAGIVRTLEWLPQNSPYRDEFVKTYREMVDALLE
                     CRRYDGFWNVSLHDPAHFGGKELTGTSLFTYGIAWGINNGILDPKKFKPIVEHSWKAL
                     VEDCLHPNGFLGYVQGTGKEPKDSQPVGYDRVPNFEDFGLGCFLLAGSEVYKLK"
     gene            3638..4990
                     /locus_tag="PLG42_04175"
     CDS             3638..4990
                     /locus_tag="PLG42_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004323914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU30442.1"
                     /translation="MKNLSYLLTLTLSLAFTIPSTAQWKNLIGKDLSNWEQLNGTAPF
                     KLEKGVIIGTTVVGSPNSFLCTKEKYSDFILEFDTWFDPRMNSGVQIRSESRPDYQNG
                     RVHGYQVEMDPSERAWSGGIYDEARRGWLYSLERNPQAKKALKVGEWNHYRVEAIGPS
                     IRTWVNGIPCADLVDDMTPSGFIALQVHSIGNDSSRAGMQVKWKNLRIITKNLDKYKT
                     PYTPVIPQVSYLNNKLTEREKAEGWRLLFDGQTSNGWMNARTGKFPESGWEIKNGELI
                     VNPETKKPGGGGDIVTVEKFKNFELIVDFKYTKGANSGIKYFVDTESEGGKYASIGCE
                     YQILDDRNHPDAKAGIGGNRTLAGLYDLIAPKNKRDNGFDQWNRAMIVVNGNKVQHWL
                     NDNLTVEYERGTPEWKALVEKSKFKGMENFGMVQEGRILLQDHGNLVSFKNIKIREIK
                     "
     gene            5127..7541
                     /locus_tag="PLG42_04180"
     CDS             5127..7541
                     /locus_tag="PLG42_04180"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU30443.1"
                     /translation="MKYLIFPFLIFILSLSGFSQTTISGRVINEDDRQPLINSTIAVV
                     KSGTNELVTGTVTNEQGRFVVPDLLPGEYDFVFSYIGFEPVKRHVISGSLNKTLDLGT
                     ILLKPSQLELAEINVTGEKQKLSSALDRKSYSVEELISGATGSVLDVVKLLPGVVIDQ
                     ESRIILRGSDKVAVLIDGKQSSLTGFGSQKGLENIPASQIEKIEVITNPSAKYDAAGM
                     AGIVNIVFKKSEQKGFSGDVGLAPGIGMITKRKKDLPTGMPSYSDNLKITPSFNLHYK
                     TEKLRIFWQSYLINQKRLPNNEFTTRFYDNGNITESQVAENRSQNHFNLKLGLDWNPS
                     PEHTITLFGLYDYEWHIDTTRVWYFGNRDYLSPLRKWGFYESEGTGFTNITLQHKFKF
                     QQPGHELNSQILFTKGWEDETYNLSQDGPPPYPVIRTDKTWVMAPEYVYMLTSDYTKP
                     LPFGRLEAGLSGRYRHMPITYTVTKDPSNTALIYNFGDWSEWNEQLAGVYANLVAEFI
                     KADIEAGMRAEYTGVSYKFAPNPYFTENEYDYFSLFPNIRLTWKVNKSNKISFFYNRR
                     VDRPGEDILRIFPKYDDPELLKIGNPSLRPQFTQNFEITYRYMWQTGSFYTSLYHKTI
                     NDYYTRVYIQDPLHSEITIKAYDNLGKAINMGAEINFEQKILKLWKLSASANIYRNTI
                     FGHSGQILFPVPQNYSVEKRSDTPFFAKLINQFSLNHGTVIEVTAQYFSAKNIAQGKE
                     LSRGGIDIGVKKSFAGNKLELNFTASDITNTMGLQQRIEGEGFRVEYQNFYETQIFTL
                     AMRYKF"
     gene            complement(7667..8971)
                     /locus_tag="PLG42_04185"
     CDS             complement(7667..8971)
                     /locus_tag="PLG42_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30444.1"
                     /translation="MKKKNLLSRRDFVQKSALGAAAFAVPSIIPARAFGAADRITVAV
                     LGVNGRGVNHIDGFSRLEGVEVVALCDPDLVVLEKRAGEFEKKYGRKVKQFQDLRKLY
                     LDKSIDAVSIATPNHWHSLAAIWAMQAGKDVYVEKPGSHNVFEGRKMVEAAKKYDRIV
                     QHGVQLRSSVAVQEAVNHLRNGLIGRVYMARGLVFRWRDDIGKKGPSPVPEGLDYDLW
                     TGPAEMLPFSRDFVHYNWHWHWNYGNGDIGNQGTHETDLCMWGLGVGLPEIISAGGGK
                     FVHDDAKITPDVLTCQFMYPKERKMIEFEVRPWITNEENGVSVGNIFYGTDGYLVVYN
                     YDRYESFLGRDKKPGPSRREGGDHYANFIEAVRKHDKSILNAPVETAHLSSALSHLGN
                     IAYRTGRTLKFDPATEKFIGDDEANKLLTRNYRAPYVVPENV"
     gene            9114..9314
                     /locus_tag="PLG42_04190"
     CDS             9114..9314
                     /locus_tag="PLG42_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30445.1"
                     /translation="MAFIGTTNRFNELRNDFLKDTGAKNVEDNLELYTLYVIARFTDQ
                     NNRLLNNLMNELQEMHKIIKRV"
BASE COUNT         2876 a         2021 c         1887 g         2750 t
ORIGIN      
        1 tgaaaggaga cgtttaccat tgtattctcc cctgttaagc agcatctgaa ggaataccgc
       61 ataatcataa acagtagaac tgagaccggc accacccgaa taatatgttc ctttgagaag
      121 cggatagttt acagcatcct gaggcatagc tcttacatga tggttctcgt cttcagtatt
      181 aaccttaaca agcctgttat gtttttcttc tggaagatag aaatatgtgt cgttcatgcc
      241 gagaggcacg aaaatatgat ttctgaagta ttcatcaagg ctttcgcccg accaaatttc
      301 aatcaagtaa cccagcacat caacgttgag gccataggtg aatctctcgc caggattatg
      361 tgcaagcggt agcttcccca gtgctctcat tttgtcacca atcaccattt tatcggtgcc
      421 gaaccctccg ggaatatcat atttggcata tatagcacgc atggttggtg agccgatacc
      481 tgcataatct attcctgatg tatgggtgaa aagatccctg attgttattt cccgttttgc
      541 aggaacagtt gtgaaggttg tatctttctc gttgaatttt tctattaccc gtgggttttt
      601 gaattccggt atatattttg aaaccggatc atcgagcagg aatttcccct cttcaaataa
      661 catcatggca gcaatgcttg taatggcttt tgtctgggaa gcaatacgga agatatcatc
      721 ttttttcaat tgtgtttttg cttccaggtc gctgacgcca aaagctttgt gatatattat
      781 tttgccatcg cgggatatga aacccacggc accggctatc cagccgctgt caacagccga
      841 ttgtatcatc ttatcaatac ggctcagacg ttccgctgac atcccggccg attcaggtgt
      901 tgcttccttc agaaccggag caggggatgt ttttgttaca ggattggtgc atgaatatgc
      961 agccagaatt aacaatgcct gaaaaagaat gaagattttt ttcataattt cctgtttatt
     1021 tatcattgat aaaattatat ctaaatttat agttgatacc tgtcaattct tatactcttc
     1081 tcttgctcct tcctacttta gcttataaac ctccgatcct gcaagcagga agcatccaag
     1141 tccgaaatcc tcaaaatttg gaactctgtc atatccgaca ggctgactgt cttttggttc
     1201 ctttcctgtg ccctgtacat atccaaggaa tccgttggga tgcaggcagt cctccacaag
     1261 tgctttccat gaatgttcca caataggttt gaactttttt gggtcgagta ttccgttgtt
     1321 gataccccat gcaatgccat aggtaaatag tgatgttcct gtaagttcct ttcctccgaa
     1381 gtgtgcaggg tcgtgtaaac ttacattcca gaatccgtca tatctgcggc actccagaag
     1441 ggcatcaacc atttccctgt aagttttaac aaattcatct ctgtacgggc tgttttgggg
     1501 aagccattca agtgttcgga ctattcctgc caggacccat ccgttacccc tcgaccaata
     1561 gcagtttctc ccgttaggtt ccttataagg agggtcaaaa tctttatcgc gccaccacag
     1621 atggtctttt gtgctgtaca atccgttatc accgtgtttt gtttttgtga acatatacat
     1681 ctcaaagagt ctgtcgaagt atctgttgtc gttgtaaaca gctcccagcc gtgcaaaaac
     1741 aggcatagcc atgtgtatca ggtctatcca gctccagtcg tcaatcttat ctgtggaaat
     1801 catattgtca atacatgctt tgatatccct tattctttcc ttttttgttg tatccataag
     1861 ataaaggtcg atgtatgtct gtccgcaaca ctggtcgtca ccgtttcttg ttgttattcc
     1921 gtttctcagt ccccatttat gaaattcgcc ccatttaatt gcataatcaa ggtatgaagg
     1981 atcgggtttg attttatata aagccatcag tccttcgtaa tacgtaccgc gtgtccagat
     2041 gttgctaggc ctttcacggt ctgtgattat tgtcttgccc acgtcgggcc atttttccat
     2101 aaaatattta ttggccagta ccattgcctg taatgttttt tcttttggga gaggcttgaa
     2161 tgttccgggg gctccggggt attttttaac ttttcccagc aggaggccgg ccaggttttc
     2221 agcagctttg cggttttcga aatctggcgt gaggcggtcg tgatcgatgt attcgttata
     2281 aatccagggg tcgccaatag caaatacata acctttaccg tagttgcatt cagctataag
     2341 cacgttgcca ttttcaacga gtacgggcct ggcatttctg gttaggctta ttgaggagac
     2401 ttctttaatg tatattttac tcacatcctt gaacagcggg tggttgggca ggtttgtgca
     2461 agcacccatt tcccagttgg ttcccgggac gggatgcagc gtaacatggt tgaatgtgat
     2521 cccgaactgt gacatcattc tgttgagatt tgtgaactca cagttgggtg cgtcatttgc
     2581 aagcactgca agtactccgc ccttctttac ccatgaagca attgtttttg catcttcctg
     2641 tgtgatatag tttggagttt tactctctgt agttgtgtcg gggtcaacga tgatatacac
     2701 attaatattt ttaagcacat ctgctgcagg cttttcaata gtggtaatta cagctccttt
     2761 gcctttaaat atttctcccc atcgtgaata tccgctccat gcggtatcgg tccagaggta
     2821 atggtagagc tggcctgttt tggggtttgt ttcgcggttg aaccagttat cgagtgccac
     2881 gactggttgt gtaaaagatt taaggcttat taaggaaaac agggtaataa ataatgtaag
     2941 agatattttt ttcatagtta tggatttttt tcaaaaataa taaagaatta catgcaacaa
     3001 cagagttggg gggcgatgga taaattatat caaggttaag ttttccataa aatggtgaaa
     3061 tttaattatt tacagatatc aaacaataac attgaaattt atgggtgttg tttcgggata
     3121 tctgagttag gttgtcacga tataatcggt aatccggatt atttcggatt ttatcaaatg
     3181 aaaaatatta tcaaatagta aaatatatta agaggtagaa cttatcaaat agtaagaatt
     3241 actaaatagt aaaaaatttt agattgtaaa aattattaaa tagaaaaaaa tattatatga
     3301 taaatattat tatatgataa aatttatcag ataataattt ttattaactg aaaaaatttt
     3361 ttatttaata tttgattctg aaaaagttct ctcattgttc tggaaactgc acacctgcta
     3421 ttcttaataa cctgcccacc ataacccggt aatcacttcc ctgacccctg cttcatgaac
     3481 cattaaaaat atctccattc tacctgccta ttattaaaga ttaaccgata acaatgaaag
     3541 atgatattat tattcttctt attaccctgt tcagtttttt attgtttttt gtatctttcc
     3601 acgcgtcttt caatagcaat tttaaaacat aaataaaatg aaaaacttaa gctaccttct
     3661 tacacttaca ctgtcacttg catttacaat accttccaca gcacaatgga aaaatctcat
     3721 aggcaaagac ctctcaaact gggaacagct taacgggacg gccccattca aactcgaaaa
     3781 gggcgttatc ataggaacaa ctgttgtggg ctccccaaac tccttccttt gtactaaaga
     3841 aaaatattct gatttcattc ttgaattcga tacatggttc gatccaagaa tgaattcagg
     3901 tgtccagatt cgcagcgaaa gccggcccga ctatcagaac ggacgtgtcc acggttacca
     3961 ggtcgaaatg gacccgtcgg agcgtgcatg gtcaggcggt atatacgacg aagcccgccg
     4021 gggatggctc tattcactcg agagaaaccc ccaggccaaa aaagccctta aagttggaga
     4081 atggaaccac taccgtgttg aggctatcgg tccctcaata agaacatggg taaacggaat
     4141 accatgcgcc gaccttgtgg acgatatgac accctcaggc ttcatagccc tccaggtgca
     4201 cagcatcggc aacgactcat caagagccgg gatgcaggtt aaatggaaaa acctcaggat
     4261 aataacaaaa aacctcgata aatacaaaac accctatact cctgtaatac cacaggtaag
     4321 ctaccttaat aataaactca ccgaacgtga gaaagcagaa ggttggagac tcctcttcga
     4381 cggccagaca tcaaacggct ggatgaacgc ccgtaccggt aaattccctg aaagcggatg
     4441 ggaaataaaa aatggggagc tgattgtcaa tcctgaaaca aagaaaccgg gcggtggagg
     4501 cgatatcgta accgtagaaa aattcaaaaa cttcgaactt attgtcgact tcaaatatac
     4561 taaaggcgct aacagcggta taaaatattt cgtcgatacc gaatcggaag gcgggaaata
     4621 cgcttcaata ggctgtgaat accagatact cgacgataga aaccaccctg atgcaaaagc
     4681 cggaatagga ggaaacagaa ccctcgctgg actctacgac ctcatcgctc caaaaaataa
     4741 acgcgataat ggattcgacc agtggaacag ggcaatgatt gtcgttaatg gaaataaagt
     4801 ccagcactgg ctcaacgata acctcacagt tgaatatgaa cgcggaaccc ccgaatggaa
     4861 agccctcgtc gaaaaaagca agttcaaggg aatggaaaac ttcggtatgg tgcaggaggg
     4921 cagaattctg ctgcaggatc acggtaacct tgtctcattt aaaaatataa aaatcaggga
     4981 aataaaataa taatatcatt ataggttttt cttcctgaat ttccaggttg ttgttaataa
     5041 atgcttttta caacaaacag gggaataaaa aagccgccca tggtgtcatt atattgataa
     5101 tagttgatca tcaaaaaata ccacagatga aatatttaat cttccctttt ctgatattta
     5161 ttttatcact ttcaggtttc tcacaaacaa ctatttccgg gagagttata aatgaagacg
     5221 acagacagcc tcttataaat tcaactattg cagttgttaa aagtggaaca aatgaacttg
     5281 taacgggaac agtaacaaat gaacaaggga gatttgttgt tcccgacctt ttgcccggcg
     5341 agtatgattt cgtcttctct tatattggat ttgaaccggt aaaacgccac gttatttctg
     5401 gttcactgaa caaaactctc gatctgggta caattcttct taagccatca caacttgaac
     5461 tggctgaaat taatgtgact ggtgaaaagc aaaagctttc ctcagcactc gacaggaaaa
     5521 gttattcagt tgaagagtta atttccggag caacgggctc tgttcttgat gtcgtaaaac
     5581 ttcttcctgg agtggtaatt gaccaggaga gccggattat actcagaggc agcgataaag
     5641 ttgctgtttt gattgacggt aaacaatcga gccttaccgg cttcggcagc cagaaaggac
     5701 tcgaaaatat acctgcatca caaatcgaga aaattgaagt aatcacaaac ccctctgcaa
     5761 aatatgatgc ggcaggtatg gcagggattg tcaatatagt ttttaaaaag agcgaacaaa
     5821 aaggctttag cggcgatgtt ggtctggcac cgggaatagg aatgattaca aaaaggaaga
     5881 aagacctccc aacaggtatg ccaagctact ctgataacct gaaaattaca ccctcattta
     5941 acctgcatta caaaacagaa aaactgcgta ttttctggca gtcgtacctg atcaatcaaa
     6001 agagacttcc aaacaatgag ttcacgacca gattctacga taacggaaat atcactgaaa
     6061 gccaggttgc tgaaaacagg tcccagaatc acttcaacct gaaactggga ctggattgga
     6121 atccttcacc cgagcatacc ataacacttt tcggattata tgactatgaa tggcatattg
     6181 atacaacaag ggtttggtat tttgggaata gagactacct ttcaccttta agaaagtggg
     6241 gtttttacga aagcgaaggc acaggattca caaatataac gctgcaacat aaatttaaat
     6301 tccagcagcc aggccatgaa ctcaattccc agatactttt tacaaaaggt tgggaggatg
     6361 aaacatataa cctcagccag gacggacctc ctccgtatcc ggttatcagg accgacaaaa
     6421 catgggtaat ggcacctgaa tatgtttata tgttaacatc cgattacact aaacctttgc
     6481 catttggaag gctggaagca ggcctctcag gcaggtaccg ccatatgccg atcacatata
     6541 cagtaaccaa ggacccgtcg aatacagccc tgatatacaa ttttggcgat tggtcggaat
     6601 ggaacgaaca acttgcagga gtatatgcca acctggtggc agaatttata aaggcagata
     6661 ttgaagcagg tatgcgggca gaatacacag gcgtgagtta taaatttgca ccgaaccctt
     6721 attttacgga aaacgagtac gattatttca gccttttccc caacataaga cttacatgga
     6781 aagttaacaa atcaaataag atatctttct tttataaccg ccgtgtcgac aggccaggcg
     6841 aggatatact gaggatcttc ccaaaatatg atgatccaga attacttaaa ataggcaacc
     6901 cttcactgcg gccacaattt acccagaact tcgaaattac atatagatac atgtggcaaa
     6961 caggttcatt ttatacatcg ctttaccata agaccataaa tgactattat acacgcgtat
     7021 atattcagga tccattacac agcgagataa caatcaaagc ctacgataat cttggaaaag
     7081 ctataaatat gggagctgaa ataaattttg aacaaaaaat tcttaagttg tggaaactct
     7141 ctgcaagtgc caatatttac cggaatacaa tattcggaca ttcagggcag atactttttc
     7201 cagtaccaca gaattatagt gtggaaaaac gctccgatac tccgtttttt gcaaaattga
     7261 tcaaccagtt ctcgcttaac catggcactg ttattgaagt tacagcccag tatttcagtg
     7321 caaaaaatat tgcacagggg aaggaactct caaggggagg aattgatatt ggagtaaaga
     7381 aatcatttgc cggtaacaaa ctggagttga actttacagc aagtgatata acaaacacaa
     7441 tgggtttaca gcagagaatc gaaggagaag gattcagggt agagtaccag aatttctatg
     7501 aaacccagat ttttactctt gccatgagat ataaattctg attgagaagg gtcaacctta
     7561 ctgctatcaa acataaatca gtaatattca ataaacagaa acaaaaaagg gccgttgcag
     7621 gagccctttt ttgctttttg aaatatatga agtacagtaa gtttcctcag acattttccg
     7681 gaaccacata aggagcacgg taattccttg taagcaattt gtttgcttca tcatcaccga
     7741 tgaatttctc ggttgccgga tcaaacttga gggttctgcc tgtacggtat gcaatattac
     7801 cgaggtgtga cagagctgac gacagatgtg ctgtttcgac cggcgcattc agaatagatt
     7861 tatcatgttt cctaacagct tcaataaagt ttgcataatg atcaccgccc tcccttcgcg
     7921 acggaccggg ctttttatcc ctgccgagga atgattcata cctgtcgtaa ttgtacacca
     7981 ccagataacc atcagtgccg tagaaaatat ttccgaccga cacgccattc tcctcatttg
     8041 taatccatgg cctcacttca aattcaatca ttttcctttc tttcggatac ataaactgac
     8101 atgttagcac gtcgggagtg attttggcgt catcatgcac aaacttgcca ccgccggctg
     8161 agatgatttc gggcaaccct acacccagtc cccacatgca caggtcagtc tcatgtgttc
     8221 cctggttgcc aatgtcaccg ttgccgtaat tccagtgcca gtgccagtta taatgcacga
     8281 aatctctcga gaaaggcaac atttctgcag gacccgtcca gaggtcataa tcaagccctt
     8341 ccggtacagg agagggacct ttctttccaa tgtcatccct ccatctgaat accagtccgc
     8401 gtgccatgta aaccctgcca atcagtccgt tacgaaggtg attcacagcc tcctgtactg
     8461 caacagagct tctcaactga accccgtgtt gcacaatgcg gtcatacttc tttgctgcct
     8521 cgaccatctt gcgtccttca aaaacattat gagaacccgg cttttcaaca tatacatcct
     8581 tgcctgcctg catcgcccat atggcagcaa gagagtgcca gtgattaggc gttgctatac
     8641 ttacagcatc aatcgacttg tcgaggtaaa gcttcctgag atcctgaaac tgttttacct
     8701 tcctgccgta tttcttttcg aattcacctg ccctcttctc aagtaccaca agatccgggt
     8761 cacataaagc tacaacctca accccttcaa gcctgctgaa tccatcaatg tgatttacac
     8821 cacggccatt aacgccaaga actgcaaccg ttatccggtc agcagcacca aatgcacggg
     8881 ccggaataat tgacggtaca gcaaatgcag ctgcacccag agcggatttc tgtacaaaat
     8941 cccttctcga taaaagattc ttctttttca taatcagatt gtttaataaa ttattttttg
     9001 aattttaaaa gcataccatt catgttgaat caggttttgc tttttcaaag ataaaattta
     9061 ttgtaacgaa catcatattt tattaacttc acaaaaagaa aaattttaag aatatggctt
     9121 tcatcggaac taccaacaga ttcaatgagc tcagaaacga ttttctgaaa gatacaggag
     9181 caaaaaacgt tgaagacaac ctggaactat atactttata tgtgatagcc aggttcaccg
     9241 accagaataa ccggctgctg aataatctca tgaatgaact ccaggagatg cataagataa
     9301 tcaaaagagt ataggagagt caggaaattc aaatcggaat tttttaatta ccgggtcttt
     9361 aacgttttaa tggctgaaac tatttgcaat acatttatct tcagcaacag cagatcttat
     9421 tatttaaaga aatttattta taacgaaatg tttagttgaa tgatttaatt gattttcaaa
     9481 catatttgaa ttatttgctt attaaaagaa tatttacatt taatctgatt gcgt
//
LOCUS       DAOSNB010000065         9380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7285_length_9380_cov_3.690617, whole genome shotgun sequence.
ACCESSION   DAOSNB010000065 DAOSNB010000000
VERSION     DAOSNB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7285_length_9380_cov_3.690617"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLG42_04195"
     CDS             complement(<1..357)
                     /locus_tag="PLG42_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOU30446.1"
                     /translation="MRLWTIHPRYLDARGLVALWREALLAKKVLEGKTRGYTNHPQLE
                     RFRKAERPIDAINQYLAVIYEEALKRGYRFDRSKIDMNFTPQKISVKRGQIGFEIDHL
                     LKKLKKRDRLKYDDIME"
     gene            1064..1564
                     /locus_tag="PLG42_04200"
     CDS             1064..1564
                     /locus_tag="PLG42_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30447.1"
                     /translation="MDLTSTIIGVTILILFLLPVYLINRVNKNKRIRLLKKLELQAKS
                     ENMNLTETDVWGDSVIGFDSEKGKMIYITEENGEERVFVFGKSDLKSFTTVPNLSRDD
                     KLNEELKKGGRLSLIFSFIQKGRPDLCIDFHIPGFGKISDKDRELFFKWRNLSRNLLP
                     FQNSKQ"
     gene            1569..2987
                     /locus_tag="PLG42_04205"
     CDS             1569..2987
                     /locus_tag="PLG42_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOU30448.1"
                     /translation="MVNNNKSVAGRIFRPTVTIIIKMSLLLIFSFIPLYAGASDGKIP
                     SLGPVRFEFIIFGLILLGVALFHNQTLKVALTGLVILIAFKLVFDSEFDFFKHIFGER
                     SFSEQIINKDLREGEWSILLNLAGLLLGFAILSRVFEQSGVPIFLLRILPGGWKGALL
                     LLFFIFILSSFLDNIAAALIGGTIASVVFKNKVHVGYLAAIVAASNAGGSWSVIGDTT
                     TTMMWIDGVSPLNILHGFIAAMGAFLFFSWFASRQQDRYQTMTTDISIKTKIDSLKLL
                     IVFMILAGAIISNFLYNMPALGVWIAILASSLIKPIPWKEVPGSIKGTIFLLSLVFCA
                     SLMPVQDLPDASWHTAFFLGLLSAVFDNIPLTKLCLEQGYYDWGMLAYTVGFGGSIIW
                     FGSSAGVAITNKFPEARHTGKWLKNGWHVAAAYLVGFTMLLLIVGWHPSDSRNHKIKD
                     LKILSYSLGSRSIENVSVSKSP"
     gene            complement(3068..4984)
                     /locus_tag="PLG42_04210"
     CDS             complement(3068..4984)
                     /locus_tag="PLG42_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HOU30449.1"
                     /translation="MKLKIFYTVIILSGVTLLTAAQNSMTISAEPRMMEHLSRGLIAV
                     RIPGDSVFISWRLLGTEPEDIAFNLYRQSGNSKAIKLNKKPITESTCFTDGTANLNIE
                     NTYFVRAIIRGKELPSDKPYTLRADTPVQQYITVPLRTPPGYTPNDASVGDLDGDGDY
                     EIVLHQTGRSIDNASPGISSIPIFQAYKLDGTFLWEINLGKNIREGAHYTQFMVYDLD
                     GDGIAEFACKTADGTIDGLGKVIGDSTKDWRNLDKASGQKYGKILDGPEYFTIFSGRT
                     GEALATTNYIPDRYPLNGWNGHGGNGGGDSTGNRVDRFLACIAYLDGVRPSVVMCRGY
                     YGRSVLAAWDWREGKLTLRWVFDSKDGENPYSGQGNHNLAVADVDGDGKDEIVYGAMV
                     VDDDGKGLFSTGYRHGDALHVGAFIPGRKGLQVFGIHEIEDKTTGPGVTVFDAATGET
                     IFRGSENIDVGRGVAADIFPDNPGAEMWWSGSDGLYTMTGKKIGPMPPSINFLIWWDG
                     DLSRELLDRTYIDKYGTGRIFTAEGCLSNNGSKATPVLSADIFGDWREEVIFRSADNS
                     NLRIYTTTIPTRHRIYTLMHDSQYRLAIATQNVGYNQPPNTSFFLGTGMSKPPKPNIV
                     TVIPQYFKGSARKN"
     gene            complement(5086..6378)
                     /locus_tag="PLG42_04215"
     CDS             complement(5086..6378)
                     /locus_tag="PLG42_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOU30450.1"
                     /translation="MKTSRRKFISAAVAGSVGLGMMPLASCKSLKQPCITPEILKERY
                     SKLDEIIRQPVLKRELLSNPVIIETVELLRYRNNFLCRVRSKDGAEGISVGHSTMSTL
                     FPIFLRNLQPFFVGQDARELDLILERVYIYNFNFRYNGISLGIPLATIEFAILDMLGH
                     IAGKPVAELIGKIHNPEVGIYVATEFREKPLEEHIDLIKKEVAKYDAKALKIKIGYQY
                     AGTKDIHYQGIPGKTEKLIPLVREIYGEEMALYADSNGYYDVEGAIRAGRILEENKYL
                     YFEEPVMHDHFEEIKAVADALDIPVANGEQDQSFVHFRWLLANDGIEIVQPDSYYFGG
                     MIRSMKVALMAHAFGKECVPHMSGGGLGFVYNTILVSALPNAGAHHEFKGLDTAVVFE
                     CPTSPLKVVNGKIKAPTGPGMGVNIDPDFIKKHELVTM"
     gene            complement(6422..7309)
                     /locus_tag="PLG42_04220"
     CDS             complement(6422..7309)
                     /locus_tag="PLG42_04220"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU30451.1"
                     /translation="MRKLFITAILSILLHPVSGQDVVRLISVEICHCIDTIENMDSLE
                     AKLDRCFPDAIDAVIGLLDENMQELFCDEATLDKTIDEVYEKFLSYCPKVRKFILEDK
                     EKSFYKMSDSQKAREFFEAGNKAFNSKDYKTAVKLYLKAIKLDRQWVLPLDNLGLTYR
                     TTGNYKKAVKYYDKSLSLYPEGRIALQNQAIAYTYLNDFLHAMNNYDQLLYLYPENPE
                     GYFGTALISFMIKDYERALDFALYSHKMYVSSQSENSKDSEKLISMIYEKMKEQGKDS
                     IFFEKAKQSGVNIRFEQVP"
     gene            complement(7318..7602)
                     /locus_tag="PLG42_04225"
     CDS             complement(7318..7602)
                     /locus_tag="PLG42_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30452.1"
                     /translation="MLEKKFYSNGQKVYDLDNNRLTYYYKNGKIKAEGPFINKMMDGE
                     WKFYRESGQLWQVANFKNNMKHGLWVRYDRNGKEEYREIFENNKAKGKKK"
     gene            7854..9176
                     /locus_tag="PLG42_04230"
     CDS             7854..9176
                     /locus_tag="PLG42_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOU30453.1"
                     /translation="MIIFFLVALSIIVVIWLTSRLRLQAFIALFIVSLLLALLTIPPD
                     KVIATLKDGFGSTMASIGFLIIFGAMIGVILDRTRATISIADFILLKTGTKRSAVAMG
                     ITGFITGLPIFCDSGFIVLSGLAKSFSRRTGVVMPFMATVLATALYSVHCLIPPHPGA
                     TAAAGIIKADMGQLVFTGILFAVPGAVSAFFWSRWISKKGKYEAYNGSGENVMVPDGK
                     LPAVFLSFLPVVIPLLLITIRSVLMMFEGLSGTFILKVMSVAGEPVTALFTGVILALF
                     LVKDKSINSFNSLLTEAIEKAGPILIVTAAGGTFGMVIKATGAGEAAGNILAGTGLGL
                     AVPFILAAILKTAQGSSTVAIITAASFVAPMLPALGLDSESGRLFAVLSMGAGSMIFS
                     HANDSFFWVVSNFGGIETGTNLKVYSSSTAIMGTVVFITIWISSFFVI"
     gene            9187..>9380
                     /locus_tag="PLG42_04235"
     CDS             9187..>9380
                     /locus_tag="PLG42_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOU30454.1"
                     /translation="MSQEIRNNRQLAEEIFLAGVDAVHPSKLMGRCLKRSEEGLIING
                     VLHRIKGKIYVAGAGKASGA"
BASE COUNT         2514 a         1846 c         1927 g         3093 t
ORIGIN      
        1 ctccataata tcatcatatt tgagcctgtc tctttttttg agctttttaa gcagatggtc
       61 tatttcaaat cctatctgac ctcttttaac agatattttt tgaggggtga agttcatatc
      121 aattttgctt ctgtcgaacc tatatcctct tttcagtgct tcctcatata ttacagcaag
      181 gtactggtta attgcatcaa tgggtctttc agcttttctg aatctttcaa gctgggggtg
      241 attcgtgtaa cccctcgttt tgccttcaag aacttttttt gccagaagcg cttccctcca
      301 taatgctacc agtccccttg cgtcaaggta tctgggatgt attgtccata gtctcatagt
      361 atataaagat atgaaaatta ttgaataaag tagtttgcag gttgatataa ttatgtattt
      421 atggtcgagg attcattgct cactggtata gtggagatat ctttcatttc tatgtattaa
      481 tataacagaa tattatttaa ttttgccacc atgattcctg ctttcagaat atttatagtc
      541 tcggtattta tttctgccac gctgatatac agcaggataa tctgttttca aacatcatat
      601 ttcattaatt actcgaatga acaacaatca catgatctgt attctcttaa ttcggttgat
      661 tttgtaattc tgagtaatac cgtaaagtct gtttttgagc aattaagtca gaaagctgag
      721 aaaaccatat tttctttaca cctctggcga aatgttcata agatcgcatt ttacggtaaa
      781 gaccttttta caagtataag agtccttaaa tcaatatttt ataagataaa aatttttagc
      841 ccacctgaca taattttccc tttcgattat tatctctgaa acctcttcat ttcaatcatc
      901 tgtaatatag ctgcttaagt gattatccat tgcaaaaatc cgttacatgg aaaagggtta
      961 atttgatgga tttaatcctt ttgtaattct caaattatgt attacatcat tttatcattc
     1021 agatttatac aaaatatgtt tttaacaatt aaaaattaaa attatggatt taacaagtac
     1081 aattataggt gtcaccattc ttattctttt ccttttgccg gtgtatttga taaaccgggt
     1141 aaataaaaac aaaagaatca gattgttaaa aaagcttgaa ttgcaggcaa aatcagaaaa
     1201 tatgaatttg accgaaacag acgtctgggg tgattctgta ataggatttg attctgaaaa
     1261 aggaaaaatg atctatatca ctgaagaaaa tggtgaagag agagtgtttg tttttggcaa
     1321 aagcgatcta aaatcattta ccactgtacc taatttaagc cgtgatgata agttgaatga
     1381 agaactgaaa aagggcggaa ggctgagcct tattttttct ttcattcaga aaggaagacc
     1441 agacctttgt attgattttc atataccggg ttttggtaaa atttctgata aggatagaga
     1501 attatttttt aaatggcgta atctttcccg gaatctatta ccttttcaga attctaaaca
     1561 gtgaaacaat ggtaaataat aataaaagtg ttgctggtag aatttttagg ccaacagtga
     1621 ctattattat aaaaatgagt ttattgctga ttttctcatt tatacccctg tatgcagggg
     1681 cgtctgacgg taaaattcct tcattgggac ctgtaaggtt tgaatttata atttttggct
     1741 taatacttct tggtgtagct ttatttcaca atcagacact taaagtggca ttaaccgggc
     1801 ttgtaatttt gattgcattt aaattagttt ttgattccga gttcgatttt tttaaacata
     1861 ttttcggaga aagaagtttt tcagagcaga taattaataa agatctcagg gaaggtgaat
     1921 ggagtatttt gttgaatctc gctggattgc ttcttggttt tgcaatactt tccagagtgt
     1981 ttgaacaatc aggtgttccc attttcttac tccgtatttt accaggtgga tggaaagggg
     2041 ctttattact attgttcttt atttttattc tttcatcttt tcttgataat atcgctgcag
     2101 ccctgatagg tggcactata gcaagtgttg tctttaaaaa taaagtgcat gttgggtatc
     2161 tggcagctat agttgctgca agcaatgccg gaggtagctg gagtgtgatt ggagacacta
     2221 ccactacaat gatgtggatt gacggtgtaa gtcccttgaa tattcttcat ggattcattg
     2281 ctgcaatggg agcattttta ttttttagct ggtttgcttc aaggcagcaa gacaggtatc
     2341 agacaatgac aaccgacata tcaattaaaa caaaaattga ttctttaaag ctactaatag
     2401 tttttatgat ccttgcaggg gctataattt caaattttct ctacaacatg cctgcactcg
     2461 gagtgtggat tgcaatttta gccagctcat tgatcaaacc aattccgtgg aaggaggtac
     2521 cagggtctat taaaggaact atattccttc ttagtctggt gttctgtgct tcactgatgc
     2581 ctgtgcagga tctgcctgat gcttcgtggc atactgcgtt ttttcttgga cttctatcgg
     2641 cagtttttga taatattcct cttacaaaac tatgtcttga gcaggggtat tacgactggg
     2701 gaatgctcgc atatactgta ggttttgggg ggtcgataat atggtttggt tcttctgccg
     2761 gtgtggcaat aacaaataag tttcctgaag cacgccatac agggaaatgg ttaaagaacg
     2821 gatggcatgt tgctgcagca tatttagtag gcttcacaat gttgctactt attgtgggtt
     2881 ggcatccttc agattcaagg aatcacaaaa taaaagattt aaagattcta agttattcgt
     2941 tggggtcaag aagtattgaa aatgtttcgg tatctaaaag tccatagcaa taatcaaaaa
     3001 aatactgtgt tgtaaacctc gattatgctt cagtgtcaat ttttaacttt gttattgatt
     3061 atagggttta attttttctt gctgaccctt taaaatattg gggaattaca gtaacaatat
     3121 ttggttttgg tggtttgctc attcccgtac caaggaagaa gcttgtgttg ggaggttggt
     3181 tataaccgac attttgtgtg gcgattgcca gtctgtattg ggaatcgtgc atgagagtat
     3241 atattcggtg cctggtagga attgttgtag tataaattct caggttactg ttatcggcac
     3301 tacggaagat aacctcttcg cgccagtcgc cgaaaatatc agcactcagg acaggggtgg
     3361 cttttgagcc gttgttcgaa aggcatcctt cggcagtgaa gatacggccc gtgccgtatt
     3421 tgtcaatata ggttctatcg agcagctctc tcgacagatc accgtcccac catatcagga
     3481 aattaattga tggtggcatg ggtcctatct ttttgccggt catggtatat agtccatctg
     3541 aacccgacca ccacatctca gcacccggat tatctggaaa gatatcagcg gctacacccc
     3601 gtcccacatc aatattctca gagccccgga aaatagtctc tcctgtcgca gcatcgaaca
     3661 ctgttacacc cggacctgta gtcttatctt caatctcatg tatgccaaac acctgcaacc
     3721 ctttcctgcc cgggatgaaa gcccctacat gcagggcatc gccatgacgg taacctgttg
     3781 agaatagtcc ctttccatca tcgtccacaa ccattgctcc gtatacaatt tcatcctttc
     3841 cgtcaccgtc gacatcggca actgccaggt tgtgattacc ctgacctgaa taaggatttt
     3901 caccgtcttt gctatcgaat acccagcgga gggtaagctt accttcgcgc cagtcccatg
     3961 ctgcaagcac cgaccggcca taataaccac ggcacatcac aacactgggg cggacaccat
     4021 cgaggtatgc tatgcaggca aggaagcgat caacacggtt gcctgtgctg tctccccctc
     4081 cattgcctcc atgaccgttc cagccgttaa ggggatatcg gtcgggtatg tagtttgttg
     4141 tggcgagagc ttcacctgtc ctgccactga aaatggtgaa gtattcaggt ccgtcgagaa
     4201 tcttgccata tttctgtccg cttgctttgt cgaggttgcg ccagtcttta gtggaatcgc
     4261 ctatcacctt tccgagcccg tcaatggtgc catcggccgt tttgcaggca aactccgcaa
     4321 taccgtcacc gtcgaggtca tataccataa actgtgtata atgagctcct tcacgaatgt
     4381 tttttccgag attaatctcc cataaaaaag ttccgtcaag tttgtatgcc tgaaagattg
     4441 gtatacttga aataccggga gaagcattgt caatactcct gccagtttga tgcagcacta
     4501 tctcataatc tccatcgccg tcaaggtcac ccactgaagc atcattggga gtatatccag
     4561 gaggtgttct cagaggaaca gttatatatt gctgtacagg agtgtcagct ctgagggtat
     4621 aaggtttatc ggatggaagt tccttgcctc ttattatggc cctgacaaaa tatgtatttt
     4681 caatattgag gtttgcagta ccgtctgtaa aacaggtgct ttctgtaatt ggttttttgt
     4741 tgagtttaat tgctttgctg tttccactct gcctgtagag gttgaaggca atatcttctg
     4801 gctctgtgcc aagaagacgc cagcttataa atacagagtc acccggtatg cgtacggcaa
     4861 taagcccgcg acttaggtgt tccatcattc gcggttctgc agaaatagtc atggaattct
     4921 gagccgcagt taagagagtg acacctgaaa gaataattac tgtatagaaa attttaagct
     4981 tcataatatt atcactgaat ttttcaggaa tattcaagta tttatcttgt aaaatatttt
     5041 ttatcaaata gttgttaaca acatctaaat aatcgaagtt tcaaactaca ttgtcaccag
     5101 ctcatgtttt ttaatgaagt caggatcaat gttgaccccc atacccgggc cggttggtgc
     5161 ttttattttg ccgttgacaa ccttcagggg tgatgtgggg cattcgaata caacagcggt
     5221 gtcgagccct ttgaactcgt ggtgcgcacc agcattgggc agtgcagaaa ccaggatggt
     5281 attatataca aaccccagcc cgccgcctga catgtgagga acacactcct taccgaatgc
     5341 atgagccatc agggcaacct tcatcgaacg tatcataccc ccgaaatagt aactgtcggg
     5401 ctgaacgatt tcaattccat cgttggctaa caaccatctg aaatgtacaa agctctgatc
     5461 ctgttcgcca ttggcaaccg ggatatcaag tgcatcggca acggctttta tctcttcaaa
     5521 atggtcatgc atcacaggct cctcaaaata gagatattta ttttcttcaa gaatcctgcc
     5581 tgcacgtatt gccccttcca catcgtaata cccgttcgaa tcggcataga gtgccatttc
     5641 ttccccataa atttccctga caagcggaat gagcttctca gtctttcccg gtattccctg
     5701 gtaatgaata tctttcgtac cagcatactg ataacctatt tttatcttga gtgcctttgc
     5761 atcgtattta gccacttctt ttttaataag gtcaatgtgt tcttccagag gcttttcgcg
     5821 gaattctgtt gcaacataaa ttccgacttc cgggttgtgt attttgccta tgagttctgc
     5881 cactggctta ccggctatgt gtccgagcat gtctagtatg gcaaactcaa tggtggcaag
     5941 cggtatgccc agggagattc cattgtatct gaaattgaaa ttgtagatgt agactctttc
     6001 cagaataagg tcgagttctc gggcgtcctg accaacgaaa aagggttgga gatttctgag
     6061 gaagatagga aaaagagttg acatggtact gtgaccaaca gagatacctt ctgcaccatc
     6121 tttagagcgc acacggcaaa gaaaattatt gcggtaacgc aacagttcaa ctgtttctat
     6181 tatcacggga ttcgataaaa gctctctttt caaaacaggt tgacggatga tctcatcgag
     6241 ttttgaatat ctctctttca gtatttcagg tgtgatgcat ggttgcttca gggatttgca
     6301 tgaggccaga ggcatcattc caagtcccac actgcctgca acggcagcag agatgaattt
     6361 acgcctgctt gttttcataa acacgataca cttatttgat aattcaatga ataattgttt
     6421 ctcatggtac ctgttcaaat ctgatattta caccagattg ttttgctttt tcgaagaaaa
     6481 tagaatcttt tccctgctcc ttcatttttt cataaatcat cgagatgagt ttttcggaat
     6541 ctttgctgtt ttctgattga gatgaaacat acatcttatg agaataaagt gcaaagtcga
     6601 gagcccgctc ataatctttt atcatgaaac ttataagggc tgtaccgaag taaccttccg
     6661 gattttcggg atacaggtac aggagctgat cgtaattgtt cattgcatga aggaaatcat
     6721 tcagatatgt atatgcaata gcctgattct gtaaagcgat acgtccttcg ggatatagac
     6781 tgagggattt gtcgtaatac tttacagctt ttttgtaatt acctgttgtg cgataagtta
     6841 ggccaagatt gtctaaagga aggacccact gcctgtccaa tttaattgct ttcaaatata
     6901 gtttaacagc tgttttgtaa tctttcgagt tgaatgcttt gtttccggct tcgaagaatt
     6961 cccttgcctt ctgggaatct gacattttat aaaaactttt ttctttatct tcaagaatga
     7021 acttccttac ttttggacag tacgataaaa acttttcata gacttcatct atcgtcttat
     7081 caagagttgc ttcatcacag aaaagttcct gcatattttc atccaatagc ccgattacag
     7141 catcgatagc atcagggaaa catctgtcga gtttggcctc aagggagtcc atgttttcaa
     7201 ttgtatcaat gcaatggcat atctcaacag aaataagcct tacaacatcc tggcctgata
     7261 cagggtgaag caaaatcgat agaatagcag ttataaacag ttttctcatt acgttgatta
     7321 tttctttttt ccttttgctt tgttgttttc aaatatttct ctgtattctt cttttccgtt
     7381 cctgtcatat ctcacccaga ggccatgttt catattgttt ttgaaatttg caacctgcca
     7441 tagctggccg ctttccctgt agaatttcca ttcaccatcc atcattttgt taataaacgg
     7501 accttctgct ttgatcttgc catttttata ataataggta agcctgttgt tatctaggtc
     7561 gtaaaccttt tgcccgttgc tataaaattt cttttcaagc atactcaaat attatttgaa
     7621 tgagtgttta aagatattca atttgataaa gaaaatgaag ctgcatggat taatctttat
     7681 gttgttttaa gtcacaattg caggatgtcc aattattgaa tctgtatttt aatctataga
     7741 aaatctatta aagctggttt gtaatcttta ttctgggagc atttgcatca tgacatgtaa
     7801 taattatctt taatccctta actggtatct gagttattga actgatgaat actatgataa
     7861 ttttttttct tgttgccctg agcataattg tggttatatg gcttacttcg aggctgcggc
     7921 ttcaggcatt tattgcgttg ttcattgttt ctctgcttct ggcattgctg actatccccc
     7981 ctgataaggt tattgccaca cttaaggacg gttttggttc gacaatggca tcaataggtt
     8041 ttctgataat ttttggtgct atgattggtg ttatactcga ccgtactcgt gcaaccatca
     8101 gtattgcgga tttcattctt ttaaaaacag gaacgaaaag aagtgctgta gccatgggca
     8161 tcacaggttt catcacaggc cttcctattt tctgtgactc gggttttatt gtgctgagtg
     8221 gactggcgaa gtcgttcagc aggcgcactg gcgttgtgat gccttttatg gcaactgtac
     8281 tggcaacagc gctctattcg gttcattgtc ttattcctcc gcatcccggt gcaacggccg
     8341 cagcaggtat aattaaagca gacatgggcc aactggtatt caccggtatt ttgtttgctg
     8401 tacccggagc tgtaagtgct tttttctgga gcagatggat ttctaagaaa ggaaaatatg
     8461 aggcttacaa tggttcaggg gaaaatgtta tggttccgga tggtaagctt ccagctgttt
     8521 ttctgtcatt tctgcctgtt gttattcctc ttctccttat caccatcagg tctgtgctga
     8581 tgatgttcga aggtctcagt ggcacattta tcctgaaggt tatgtctgtt gccggcgaac
     8641 ctgttactgc tctttttact ggtgttattc ttgccctttt tctggtgaaa gataaaagta
     8701 ttaactcctt caatagcctg cttactgaag ccattgaaaa ggcaggtccg atacttatag
     8761 ttacagcggc aggggggaca ttcggaatgg ttatcaaagc cacaggggcg ggtgaggcag
     8821 caggaaatat ccttgcaggg acgggtctgg gtcttgctgt accatttatt cttgctgcaa
     8881 tattgaaaac agcacagggc tcttccacag tggcaattat cacagccgca tcttttgtgg
     8941 ctcctatgct tccggctctg ggactcgact cagaatcagg caggctattt gctgtgctgt
     9001 cgatgggcgc tggctcaatg atattttcac atgcaaacga ctcattcttc tgggtggtgt
     9061 caaattttgg cggaattgaa accggtacaa acctgaaagt ctattcatct tcaactgcaa
     9121 taatgggaac agtagtattc ataacgattt ggatttcatc tttttttgtg atatgaatta
     9181 ttgaccatgt ctcaagaaat acgaaataac agacaacttg ccgaagagat attccttgcc
     9241 ggagtagatg cagttcatcc ttcaaaactg atgggcagat gtctgaaacg ctcagaagag
     9301 ggattgataa taaatggagt tctccacagg ataaaaggta aaatctatgt ggctggtgca
     9361 ggcaaagcgt cgggtgcgat
//
LOCUS       DAOSNB010000066         9149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7533_length_9149_cov_3.996811, whole genome shotgun sequence.
ACCESSION   DAOSNB010000066 DAOSNB010000000
VERSION     DAOSNB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7533_length_9149_cov_3.996811"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="PLG42_04240"
     CDS             <1..1734
                     /locus_tag="PLG42_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30455.1"
                     /translation="GYSAGLLVLLLFAVSPTFLGHSQNNLKDIPFALGYISGIFFTLR
                     FLFSDKKLNLFDIILLILSIAFCMSIRAGGMMLIVYLLAFFFLTEFIRYFNLRTINLV
                     ESGRKFGIIILISVAAWFLSVVFWPYGLQNPIINPVKAHIVMSRFPLTFREIFEGRTE
                     WTDFMPWYYLFKYMLITFPLTVVAGLVFLVVYLPCKVKIPKFTEYMLLSLTIILPILY
                     EIIFKPNIYSGWRQFLFLYPGIVIFSAKGINGLFSIVPGRYKLVMVLLLLLTALHPLK
                     FIITDFRYAYIYFNPVVGGLKGANGNYETDYYFIGHREAAEWLGSYLQSKNVKDTVIV
                     GSNFSTAWYFRNNPLVKNITFRNEERSNFDWDYYLSTNRYILPFRLKNGEWPPQGALK
                     VVYADGAPVCAVIERKSKDSYYGFKALQERRVGDAVNLFKKAVEENGNDEMIFYNFAV
                     ALNRAGDSLKADSVLMKSLEINPFFEPALMYAGMISESKGRYDEAEGYYKRLIEINRK
                     YSEAYVRLAGIVERKDVVRARAILRECLRINPVYRTALIRLADTYRGSHPEIARKYDS
                     LAAKIGSGIKY"
     gene            1772..2044
                     /locus_tag="PLG42_04245"
     CDS             1772..2044
                     /locus_tag="PLG42_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30456.1"
                     /translation="MKKLLLTLFLSALLVVTGIAPVVKAQEEPKPKKDSVNMDTIAKP
                     VFYYAIEDEESMGKKGGKGAASTIVVIAGAVIIVGGVVFSLLKKKK"
     gene            complement(2151..3179)
                     /locus_tag="PLG42_04250"
     CDS             complement(2151..3179)
                     /locus_tag="PLG42_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU30457.1"
                     /translation="MGTTAAAASVFFIPSLFGSEAKQKIVKPNSNFGGVKIGAITYSW
                     RSMPGGVENIIKYCQESGISNIELMSNDLESFLGAPQNPMAGMMRPPAGQQQPGQPGQ
                     PGQQGQPGGQPGGQRPQRTPEQEAAIAKYNEELKKWRTSLPMSKVQQARKLFDDAGIS
                     VHIVKFSPARWSDEEIDYAFKVAKAMGAKGVTDEIGEEAVKRLAPFAEKHGMYAIFHN
                     HMQFGTPGFSYDPFLAVSPAVMMNFDAGHFFGSTGIHPNEILQKYHDRIFSVHMKDKT
                     GPKSTPPNTNVEWGKGETPIADVLLLIKKQKWPIHVDIELEYPVPEGSDAVKETKKCV
                     EFMRKILA"
     assembly_gap    3218..3317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3438..4574)
                     /locus_tag="PLG42_04255"
     CDS             complement(3438..4574)
                     /locus_tag="PLG42_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30458.1"
                     /translation="MKKIKLGMIGGGIGAFIGDAHRRASRICNDFELVGGVFDADYEK
                     SKEFALKEGIPLERCYESVEAMIKGELARAEGDRMQAVSIVTPNYLHYPFAKMLLENG
                     FHVMCEKPMTMTVEEAEELERLVNKNKLTFALTHTYTGYPMVRQMREMIRSGMLGTIQ
                     RIDAQYYQGWVNSIIHGADSRITGVWRFDPAQSGVSSCMGDIGVHAFNLIEYTTGLEI
                     KQVLSDLNNVKEGFQMDLDGTVLLRFENPKLKGVIRSCQVATGEENNLTIAVYGSKAS
                     LKWGQENPNYLYLLSDAEPVKVLKPAHSYNSEFAEKSHTMPCGHPEGIYEAIANIYKG
                     MAKSIRGEQFTPGEFPTVHDGVRGMKFIHAVVKSYNSGNVWVTI"
     gene            complement(4634..5629)
                     /locus_tag="PLG42_04260"
     CDS             complement(4634..5629)
                     /locus_tag="PLG42_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU30459.1"
                     /translation="MSRPVTLFTGQWADLKLEDLCKKAKDFGYDGLELACWGDHFEVS
                     KADAAYCKAKKDLLAKYGLKVYAISNHLVGQAVCDRIDQRHKSILPDYVWADGYPDKV
                     NERAAQEMIKTAEAAARLGVEVVNGFTGSPIWHLLYSFPPVPQSMIDEGYEIFASRWI
                     PILDRFKQLGVKFALEAHPTEIAFDIPSAQRALKALNNHPAFGFNFDPSHFGYQGVDY
                     IKFIYTFSDRIFHAHMKDVYWSDVPTDAGVFGGHTEFGTKGRFWDFRSLGRGKINFEE
                     IIRALNRIGYKGPLSVEWEDSGMDREHGAKEAAAFVRKIDFPPSAVAFDAAFERK"
     gene            5913..8351
                     /locus_tag="PLG42_04265"
     CDS             5913..8351
                     /locus_tag="PLG42_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30460.1"
                     /translation="MKGFKISGFFLFVLLTVLSNIIDAQVVRREVINFNFDWKFYKGD
                     IPGASAQDFDDAGWRILDLPHDWSIEGPFDARWASGTGYLPGGIGWYRKTFNVPKNWS
                     RGRVFILFEGVYNNSEAWINGNYLGKRPNGYISFQYDITPYLRYGKNNTIAVKVDHSK
                     YADSRWYTGSGIYRNVKLIFTPEVHIANWGVFYATPFVSKESAVMNISTNIKNESRKK
                     AGAEVTNTLYSGDKIVASVSSSITIESMQESLVEQKLEVKDPVLWSLDTPHLYRLVTT
                     VKGKGFTDENIMNAGIRFFSFDASRGFNLNGISMKLKGVCLHHDAGTLGAAVPRSEIE
                     RRLGLLKEMGCNAIRTSHNPYSPDFLDVCDSMGFLVINEAFDEWELPKKKWIEGWNRG
                     TPGKDGYAEYFKDWALRDLADFVLRDRNHPSVIMWSIGNEIDYPNDPYTHPVLNTEAN
                     PQTWAKYDEKLPHANRLGEIARELVATVKKYDTTRPVTAGLASALMSNETGYAAALDV
                     VGYNYQEFRYERDHKAYPDRPLYGSENGMSLEAWKAVEENDYVAGQFLWTGIEYLGEA
                     GQYPSRHSTSGLLDLAGNRKPEYYFRQSLWSSKPMVFLGTTTSVTTREPANLWAHKRV
                     DPVWNYNEGQMVSVNAFTNCEKVELFLNGKSLGSKNLADFPNRTISWDVPFEPGELKA
                     TGKIDGIEVASYSLNTYGKPAAIRAMCDAMVLKANRTDISRIYVTVTDEKGNVVYNAE
                     NEIVCEITGPVRLLGMEDANPRNTENYKDNRQKAYHGRLLIYLQSLDTPGKANIKISS
                     EGLNSLTFTIDLVK"
     gene            complement(8362..8883)
                     /locus_tag="PLG42_04270"
     CDS             complement(8362..8883)
                     /locus_tag="PLG42_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30461.1"
                     /translation="MKTKTFLRKTETFEPSFLLITVAAILMSLSVTRSNAQVNFSGTW
                     VLNEAKSSPAEGGFRFAPTTINITHNGNELSVQSTMPGPDGDFTFSSKYTLDGKECSN
                     VLFQDNVRKSVVQWSADKKSLTFTHTMNFNGNEFKTTETWTLNETDKTLAIENVFNTP
                     NGEMRMTNVYDKK"
BASE COUNT         2485 a         1766 c         1979 g         2819 t
ORIGIN      
        1 ggttactctg ccggactttt agttctgctt ctgtttgccg tttctcctac atttctgggt
       61 cattcacaga ataaccttaa agatataccc tttgcgcttg gctatatttc tggaattttt
      121 ttcacactca gatttttatt ttcagataaa aaactaaacc ttttcgatat tatacttcta
      181 atactcagta ttgctttctg catgagcata agggcaggag gaatgatgct tatcgtatat
      241 cttctggcgt ttttctttct taccgagttt atccgttatt ttaatcttag aacgataaat
      301 ttagttgaat caggaaggaa attcggaata attattctga tttctgtggc agcatggttt
      361 ctgagcgttg tattttggcc ctacggtttg cagaatccga taattaatcc agttaaggct
      421 cacatagtga tgagcaggtt tcctttgacc ttcagggaaa tatttgaggg aagaactgaa
      481 tggaccgatt ttatgccctg gtattatctg ttcaaatata tgctgattac atttccttta
      541 acagtggttg caggtctggt tttcttggtt gtctatcttc cctgtaaggt caaaataccg
      601 aagtttacgg agtatatgct gctttctcta actattattc tgccaatact ttatgaaata
      661 attttcaaac caaatatata cagcggctgg aggcaatttc tatttcttta tcctggaata
      721 gttatctttt ccgctaaagg cattaacgga ctcttcagta ttgttccggg aagatataaa
      781 ctggttatgg ttcttctgct tcttcttacc gctctacatc cgttaaaatt cattattact
      841 gatttccggt atgcatatat ttattttaat cctgtagttg gtggtcttaa aggtgcgaat
      901 ggtaattatg aaactgatta ttattttatc gggcatcgtg aagcggcaga atggctcggc
      961 agttatcttc aaagtaaaaa tgtgaaggac actgtgatag tgggctcaaa tttttcaacc
     1021 gcatggtatt tccgaaataa tcctttagta aagaatatca cttttcggaa tgaagaaagg
     1081 agtaattttg actgggatta ttatctttca acaaacaggt atattcttcc atttagatta
     1141 aaaaacggag aatggccacc gcaaggtgca cttaaagtcg tttatgctga tggtgcccct
     1201 gtttgtgctg ttattgagag aaaatcaaaa gattcatatt acggatttaa ggctttgcag
     1261 gaacgcagag tgggagatgc ggttaatcta tttaagaagg ctgttgaaga aaatggtaat
     1321 gatgaaatga ttttttataa ttttgctgtg gctttgaaca gggcaggaga ttcgttaaag
     1381 gcagattctg ttctgatgaa gtcactggaa atcaatccgt tctttgaacc ggcattgatg
     1441 tatgctggaa tgatttcaga gtcgaaaggg aggtatgatg aggctgaggg ttattataaa
     1501 agactgattg aaatcaacag gaaatattct gaggcatacg tcaggcttgc tggtatagtt
     1561 gaaagaaaag atgtcgtaag ggcacgtgcc attttgaggg aatgtctgag aataaatcca
     1621 gtttatagaa cggcacttat caggctggca gatacataca ggggatcaca tcctgaaatt
     1681 gcccgaaaat acgacagtct ggcagcaaaa attggttctg gcattaaata ttgaagattg
     1741 gatacattaa ttaaataaag ggaaattcgt tatgaaaaaa ttattattaa ctctgttttt
     1801 atcggcactg ctggtagtta ctggaattgc accggttgtt aaagctcagg aagagcccaa
     1861 gccaaagaag gattcagtaa atatggatac aattgcaaag cctgtatttt attatgcaat
     1921 tgaagatgaa gaatcaatgg gcaagaaagg tggcaaaggt gctgcttcga ctatagtagt
     1981 aattgctgga gcggttatta ttgttggagg tgttgtcttt tcactcctga agaaaaagaa
     2041 ataaagcgac cggtaactgc tttaaatgaa aacaaaaccc tgtgctgggc ctgtgaataa
     2101 aaaaataatc aggtgagcac agggtttttt gtgtattttc ttaacattta tcaggcaagg
     2161 atctttctca taaattcaac gcactttttg gtttccttca cggcatccga gccttcaggc
     2221 actggatatt cgagctcaat atccacatgt atgggccatt tctgtttctt gatcagaagc
     2281 agcacatctg caataggtgt ttctccttta ccccattcca catttgtatt ggggggagtg
     2341 ctcttcgggc cggttttgtc tttcatgtgg acgctgaaga ttctgtcgtg atacttctgc
     2401 agtatttcat ttggatgtat gcctgttgag ccaaagaaat gaccggcatc gaagttcatc
     2461 attactgcag gtgatactgc caggaatgga tcgtagctga agccgggtgt tccaaactgc
     2521 atgtgattgt ggaaaatggc atacattcca tgtttttctg caaaaggagc aagccttttt
     2581 actgcttctt ctccgatttc atctgttaca ccctttgcgc ccatcgcctt agccacttta
     2641 aaggcgtaat caatctcttc atccgaccag cgggcgggag agaattttac tatatgcacg
     2701 cttatgcctg cgtcgtcgaa tagcttacga gcctgttgaa cttttgacat tggcagtgag
     2761 gtgcgccatt ttttgagttc ttcattgtat ttggcaatcg ctgcttcctg ctcaggggtt
     2821 ctctgtggtc tctgtccacc tggttgtcct cccggctggc cctgctgccc cggttgtcca
     2881 ggctggccag gctgttgctg cccagcaggt ggcctcatca ttccagccat cgggttttgt
     2941 ggtgcaccga ggaaactctc aaggtcgttg ctcatcagct caatattgct tattccggat
     3001 tcctggcagt acttgataat attctccaca ccacccggca tggaacgcca gctgtaggta
     3061 atagctccta tcttgacacc gccgaaatta gagttgggtt ttactatctt ctgctttgcc
     3121 tcagaaccaa atagagaggg aataaaaaat actgaagcgg ccgcagcagt tgttcccaga
     3181 aactcccttc tggaaaagtt tttttcattt tttctgtnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnngca gcagttgttc ccagaaactc ccttctggaa aagttttttt
     3361 cattttttct gttcttcatg ttctttttaa tttgtctgat taataaataa aattttataa
     3421 ataatttttg ttgattttta gattgttacc cacacgttgc ccgagttata cgattttacg
     3481 actgcatgta taaatttcat acctcttacc ccatcgtgaa cagtcgggaa ttcaccgggt
     3541 gtgaactgtt ctcctctgat ggatttagcc atgcctttgt agatgtttgc tattgcttca
     3601 taaattcctt cggggtggcc gcatggcatt gtatggctct tttctgcaaa ttcactgtta
     3661 tatgagtggg caggtttcag aaccttaacg ggttctgcat cgctcaaaag ataaagataa
     3721 ttaggattct cctgacccca tttcaggctt gctttggagc cgtaaacagc tattgtaagg
     3781 ttattttcct cgccagtggc aacctggcat gagcgaatta cacctttaag tttcgggttt
     3841 tcgaaacgaa gcagcactgt accgtcgagg tccatctgaa agccctcttt tacattgttc
     3901 aggtccgaca gcacctgttt tatctcaaga cctgtggtat actcaatcag gttgaatgca
     3961 tgtactccga tgtcgcccat gcagctgctc acacccgact gtgcaggatc aaaccgccac
     4021 acaccggtaa tgcgactgtc ggcaccatgg attattgaat tgacccagcc ctgataatac
     4081 tgtgcatcaa ttctctgaat tgttccaagc atgcccgacc tgatcatctc cctcatctgc
     4141 cttaccatgg gatagccggt gtatgtatgg gtgagggcaa aggtgagttt gttcttattc
     4201 accagccttt caagctcttc tgcttcctca acagtcattg tcatgggctt ttcacacatg
     4261 acatggaaac cattttcgag aagcattttt gcaaatggat aatgaaggta gttcggtgta
     4321 actattgata ctgcctgcat cctgtcccct tcagcccgtg ctagctctcc ctttatcata
     4381 gcctccacac tctcgtagca ccgttcaaga gggattccct ctttcaaagc aaattccttg
     4441 cttttttcgt aatcggcatc gaataccccg ccgacaagct caaaatcgtt gcatatcctg
     4501 gaggcacggc ggtgagcatc acctataaat gcacctatgc ctccgcctat cattcccagt
     4561 ttaatctttt tcatgacctt tttgttttaa agttcagaca atcagcatta taaaagcagg
     4621 taactcctga cggtcatttc ctttcaaatg ctgcgtcaaa tgctacagca gatggtggaa
     4681 agtcaatctt tcttacgaat gctgcagctt cttttgctcc atgttcgcga tccataccac
     4741 tgtcttccca ttcaaccgag agaggaccct tataaccgat tctattcaga gctctgatga
     4801 tttcctcgaa gttaatcttt cctcttccca gacttctgaa atcccagaaa cgtccttttg
     4861 tgccgaattc ggtatggcct ccgaaaacgc cagcatcggt aggcacatcc gaccagtaaa
     4921 catctttcat gtgtgcatgg aagatcctgt cggagaaggt gtaaatgaac ttaatgtaat
     4981 caacaccctg gtagccgaaa tgagatggat cgaaattgaa accgaatgca ggatggttgt
     5041 taagtgcttt caatgccctc tgggcagaag ggatatcgaa tgcaatctca gttgggtggg
     5101 cttcaagggc aaattttaca cccagttgtt taaacctgtc gagtattggg atccacctcg
     5161 aagcaaatat ttcataaccc tcatcaatca tcgactgagg tacaggcggg aatgaataaa
     5221 gcagatgcca gatagggctg cctgtgaaac cgttaacaac ttcaacgcca agccttgctg
     5281 ctgcttcggc tgtctttatc atctcctgtg cagccctttc attaacttta tcaggataac
     5341 catcggccca tacgtaatcg ggcagaatac tcttgtgccg ctggtctatc cggtcgcaaa
     5401 ctgcctgacc tacaagatga tttgaaatgg catatacttt aagcccgtat tttgcaagta
     5461 aatccttttt tgccttgcaa tatgccgcat ctgctttgct gacctcaaaa tggtcgcccc
     5521 agcatgctaa ttccaaaccg tcatatccga agtcctttgc ctttttacaa aggtcttcca
     5581 gctttaaatc ggcccactgg cctgtgaaaa gtgttactgg tctgctcata agtcactagt
     5641 tttaaggtta ttatcttaaa ttggctttat atttctatct tttagtccct tatccggcta
     5701 ttaccccggt agtcagacat ctgtttttca aatataaatt taaaaaattg caaaaaactc
     5761 ttcttaattg gtcaattttg acagggttaa cattctttaa ctatagcaat gctgaaattg
     5821 tacctaaaaa tacaagaatt actgaaatca atgatatatg tttgttttca aaatagctat
     5881 ttttagaaaa taaattagaa aaataaaata agatgaaagg ttttaagatt tccggatttt
     5941 ttctctttgt cttgcttaca gtgttaagca acattattga tgctcaggta gtaaggaggg
     6001 aagttataaa cttcaatttc gactggaagt tttacaaagg ggatattccc ggagcttcag
     6061 cacaggattt tgatgatgcc ggctggagaa ttcttgatct tcctcacgac tggagcattg
     6121 agggaccatt cgatgcaaga tgggcaagcg gaacgggtta ccttccgggt ggtattggat
     6181 ggtacagaaa aacattcaat gtaccaaaga attggagcag gggcagggtt tttattcttt
     6241 ttgaaggtgt ttataacaac agtgaagcat ggattaacgg taattacctt ggcaagaggc
     6301 caaacggtta tatttctttt caatatgaca tcactcctta cctgcgttat ggaaaaaaca
     6361 atactattgc agttaaagta gatcactcaa aatatgctga ttcgagatgg tatacaggct
     6421 cgggcattta ccgtaacgtt aaactgatat tcactcctga agtccatatt gctaactggg
     6481 gagtatttta cgcaacgcca tttgtgagca aggagagtgc tgttatgaat atcagtacca
     6541 atattaagaa tgaaagcagg aagaaagcag gagcagaagt aactaatact ctttattctg
     6601 gagacaagat tgttgcaagt gtttcgtctt ccataactat tgaaagcatg caagaatcac
     6661 tggttgagca gaaacttgaa gttaaagacc cggttttgtg gagtttggat actcctcatt
     6721 tgtacaggct tgtcaccacg gttaagggta aaggtttcac ggatgaaaat ataatgaatg
     6781 caggtattag atttttcagt tttgatgcca gcagagggtt caatcttaat ggcataagca
     6841 tgaagttgaa gggtgtatgt ctgcatcacg atgccgggac tcttggcgct gctgttccaa
     6901 gaagtgagat tgaaaggcgt cttgggcttc ttaaagaaat gggttgcaat gccatcagga
     6961 caagtcataa tccttattca cctgattttc ttgatgtgtg cgacagcatg ggttttctgg
     7021 tcatcaatga agcttttgat gaatgggagc taccgaagaa aaaatggata gagggctgga
     7081 acaggggcac acctggaaag gacggctatg ctgaatattt caaagattgg gcattaagag
     7141 atcttgctga ttttgtgttg cgcgaccgga accatccatc ggttataatg tggagcatag
     7201 gtaatgagat agattatccg aacgatcctt atactcatcc tgtacttaat actgaagcca
     7261 atcctcagac atgggcaaaa tatgatgaaa aactcccgca tgcaaatcgt cttggtgaaa
     7321 ttgcccggga gcttgtggca acagtaaaaa aatatgatac tactcgtccg gtaacagcag
     7381 ggcttgcctc cgctcttatg tcaaatgaaa ccggatatgc ggcagccctc gatgtggtgg
     7441 ggtataacta tcaggagttc agatatgaga gagaccataa agcttatcct gacaggcctc
     7501 tttacgggag cgaaaatggg atgagtctgg aagcatggaa agccgttgaa gagaatgact
     7561 atgtggcagg gcagttttta tggacaggaa tcgagtatct tggagaagcc gggcagtatc
     7621 cttcgcgcca cagcacatcc ggtttgttag atctggcggg caacagaaaa cctgaatatt
     7681 acttccgcca gagcctctgg agcagtaaac cgatggtgtt tcttggtaca acaacgtctg
     7741 taactaccag agaacccgca aacctgtggg ctcataaaag ggttgaccct gtatggaatt
     7801 acaatgaagg gcagatggta agtgtaaatg cttttacaaa ctgcgagaag gtggagcttt
     7861 ttcttaacgg aaaatctcta ggctcaaaaa atcttgctga ttttccgaac aggacaattt
     7921 catgggatgt tccttttgag cctggcgaac tgaaggcaac tggcaaaatt gatggcattg
     7981 aagtagcatc atattcttta aatacatacg ggaaaccggc tgcaataagg gcaatgtgtg
     8041 atgcaatggt attgaaggca aaccggacag acatttcaag gatttatgtt actgtaacag
     8101 atgaaaaggg gaatgttgtt tataacgctg aaaatgaaat agtctgtgaa attacaggtc
     8161 cggtaagact tcttgggatg gaagatgcaa atcccagaaa tactgaaaat tataaagata
     8221 acagacagaa agcatatcat ggccgactgc tgatttatct gcagtcgctt gatacaccgg
     8281 ggaaagccaa tattaagatt tcttcagaag gtcttaatag cctaacattt acgattgacc
     8341 tggtcaaata ggcaatgcag gctatttttt gtcgtataca tttgtcattc gcatctcacc
     8401 atttggtgta ttaaacacat tttctatggc aagtgttttg tctgtttcgt tcagtgtcca
     8461 ggtttcggtt gttttgaact cattgccgtt gaagttcatg gtatgagtga atgtcaggga
     8521 ttttttatcg gctgaccatt gcactaccga ttttctcaca ttgtcctgga acaggacatt
     8581 ggagcactct ttgccatcca gagtatattt gctgctgaat gtgaaatcac catctggtcc
     8641 cggcattgtg ctttgaacgg aaagttcatt gccgttgtgg gttatgttta ttgtagtggg
     8701 ggcgaacctg aagccacctt ctgcaggaga actttttgct tcattcagaa cccatgtacc
     8761 tgaaaaattt acctgagcat ttgacctggt tactgacagg gacatcaaaa ttgctgcaac
     8821 agttatcaga agaaaggatg gttcaaacgt ttcagtttta cggagaaaag tttttgtttt
     8881 cataggcaga tgacttttaa ggttttttag ttgaattttc catataaaaa taggatattt
     8941 tttgaatggt ttacctgtgg aaattttcgg tgaatatttc aattaaaatt tatttattaa
     9001 actgtttcac atccagcagt ttaatgttat tattatctgg tttgttttct tttgattagt
     9061 tttaattgtg gacgaactgg cagggtatgg ccggtagtat taagtagtat taaatagaaa
     9121 aatttattaa atgataaaca ttatcaaat
//
LOCUS       DAOSNB010000067         9116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7572_length_9116_cov_5.089615, whole genome shotgun sequence.
ACCESSION   DAOSNB010000067 DAOSNB010000000
VERSION     DAOSNB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7572_length_9116_cov_5.089615"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLG42_04275"
     CDS             <1..680
                     /locus_tag="PLG42_04275"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU30462.1"
                     /translation="KRFTYCFIPDFPGEINLSGRLSHWCRLPENARFIGILSRFQSFP
                     EPLSPSIKLLPTDISRREEDISYIYCTAIMSGPSPQKELLASKIKDILLKTGKNSVIL
                     SGNISSESIIQKEDNIQIINHLPEDEFAEIVGRSEIIISRSGYTTVMELFSLGRSAIL
                     IPTPGQPEQEYLAEYLSGKRWFIHLNQRNLSTNTMFELPDLTLPKFNASENEKLLNAA
                     LEELLKE"
     gene            complement(666..1301)
                     /locus_tag="PLG42_04280"
     CDS             complement(666..1301)
                     /locus_tag="PLG42_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30463.1"
                     /translation="MRDIFSEKFQLYTAKAITILFHPLFMPAYGLLIIFTAPTLLFYL
                     PGGIKKIILLILLINNVLLPLGMILVFRYRNYISSWTMEKNSERIFPLLTVSILYFIT
                     SFVIIGLSIPLFIKAYFESVSILSLIIFLINFRYKISVHSAGAGALTALVTVLWIVMK
                     ENLVWFLVPVLLISGMILAARLKLNAHNPQEVYSGFLVGFSVVSAAMLFLQ"
     gene            complement(1301..2767)
                     /gene="rpoN"
                     /locus_tag="PLG42_04285"
     CDS             complement(1301..2767)
                     /gene="rpoN"
                     /locus_tag="PLG42_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOU30464.1"
                     /translation="MLKQRLEQKLLQKLSPQQIQMIKLLEIPTVQMEQRIKKELEENP
                     ALEEGPEEDDEETIMKELEGDDFEDNEQEQEEFTLDDYIDDDEDIPEYRLQAKNYSRD
                     DEKRPEIPFSIGTTLHENLATQLGLRELTEKQKTIGEFIIGNIDEDGYLRRDISNIVD
                     DLAFLQNVNATENEVKEVLEVVQDLDPPGVGARNLQECLILQIKRKDQSNPSVRLAKK
                     ILEDHFEEFTRKHYDKIESKLGVGEDELKKAIEEIMKLSPKPGSLMSESSSETAQQII
                     PDFILEVTDNGFELHLNSRNLPELRLNKAYTEMLRNYTRDSGKKKEMKDAIMFVKQKI
                     DSAKWFIDAIKQRQNTLLLTMNAILKYQEEYFKEGDETKLKPMILKDIAEMTGLDIST
                     VSRVASSKYIQTHFGIFPLKYFFSEGLQTDSGEEVSTREIKKILQECIENEDKRRPLT
                     DEKLTEILQQKGYQIARRTVAKYREQLNIPVARLRKEI"
     gene            complement(2797..4185)
                     /gene="asnS"
                     /locus_tag="PLG42_04290"
     CDS             complement(2797..4185)
                     /gene="asnS"
                     /locus_tag="PLG42_04290"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOU30465.1"
                     /translation="MKRTKVKELLTGFPVGEEVLVKGWVRTKRSIKEIVFIALNDGST
                     IKNIQIVAEGVKFGEQLLKDINTGAAIAVKGKLAESKGKEQPVEIIAEEIELYGKADP
                     EIYPLQKKGHSLEFLREIAHLRFRTNTFGAIFRIRHAIAFAIHKYFNDKGFYYLHTPI
                     ITGSDCEGAGEMFRVTTLDFDNIPRNQEGKVDFSKDFFGKETNLTVSGQLEGELGALA
                     LGEIYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDINDNMDLAEDFLKYLTRYALEN
                     CRDDLEFLNNMFDKGLIERLEFILKNPFERITYTNAIEILKASGRKWEFPVEWGRDLQ
                     AEHERYLVEEYFKRPVIITDYPKEIKAFYMKLNDDGKTVKAMDVLFPGIGEVIGGSQR
                     EEDYNKLLNRIRELNMNEKDLWWYIDTRRFGSAPHSGFGLGLERFLIFLTGMTNIRDV
                     IPFPRTPRNAEF"
     gene            4442..5302
                     /locus_tag="PLG42_04295"
     CDS             4442..5302
                     /locus_tag="PLG42_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOU30466.1"
                     /translation="MEQNQKLIVVPWDFTHVAEHALAHAVKISRMVSNQICLLHIVDP
                     KIKPAEEKAKSESLKHIAEENSKKYNVPITSHIEKGTIFKSIGAFVNDTEKRDVSLVV
                     MGTHGIKGMQKLTGSWALKVIVKSKVPFIVVQDPPRDWERYHNIVCPMDFRSENKEKI
                     KMAIFMGKYFDSKIHIIKTTPTDKMLIKKTNINLNFAIKFLIQNNIEYEIHDLPKGDF
                     AQQTIDFAQKINADLILIVTTKNITFTDYMLGASEQYIIANSSKIPVICVNPRASYAK
                     SSQFMGGWSD"
     gene            complement(5304..6959)
                     /locus_tag="PLG42_04300"
     CDS             complement(5304..6959)
                     /locus_tag="PLG42_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOU30467.1"
                     /translation="MENQLQIVIVLIAYLSLLILWGIYQGRKVKTGSDYSIAGRKLPG
                     WVAALSERATGESSWALLGLPGAAYATGLTEIWTAIGCVAGIITAWALLAWRLRDEAE
                     KYNVTTFSDYLAARHGDTGRLLRVISSLTIVLFFFFYVGAQFLGGGKTLHTLFGIEPG
                     LGMLITALIIIPYTIYGGFRSVVYTDVIQALLMITALVIAPVAGIIYISNHPELYASG
                     IGEALRKSGESYTSLTGVAKGFGAGLLITGGFSWFFGYLGGLPQLTMRFMAIRNREQA
                     KKGRNVGIVWTIVAYTGALMIGWIGIAIFGPAGLGDPEYVMPSVILKLFPPVIAAILI
                     TGAIAAMISTADSLLILSATELSGSLLKKGEKHQDTSPAVLRKHRLITGIIAVIALLL
                     SYVSPSKIIFTLVSYVWAGIGCTFSVVILLTLFWKRFHGKAAVATIVTGILFTIFWVS
                     GGFEQHYRVTSEKADFLASQNIISNHEKEQLLVLEGRKFVNSARLADKIREQTGWSSE
                     EKKIAVVSSAFTVKSVPARLTTFIACLVAAVISVLLMPQHKDK"
     gene            7081..7992
                     /gene="gldA"
                     /locus_tag="PLG42_04305"
     CDS             7081..7992
                     /gene="gldA"
                     /locus_tag="PLG42_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOU30468.1"
                     /translation="MSITVERVTKYYGTQKALDNLSFEVKTGEIVGFLGPNGAGKSTM
                     MKIITGFIPPSSGRVLLNGIETGCDDPEIRRKIGYLPENNPLYPEMYVREYLGFVASF
                     YLSGKERQKAVDEIIELTGLVPEQNKKIGSLSKGYRQRVGLAQALIHNPEILILDEAT
                     SGLDPNQIVEIRSLIKEAGKEKTVLISTHIMQEVEAICSRIIIIDRGTIVADDPKEKI
                     YSRIMQAIQTVKVEFDRQPDEKELASIPGVISVSRTGSNMFIIEAESKADIRPHIFSY
                     AVNNNLTVLSLIRQESNLEEVFRKLTA"
     gene            8165..>9116
                     /gene="metK"
                     /locus_tag="PLG42_04310"
     CDS             8165..>9116
                     /gene="metK"
                     /locus_tag="PLG42_04310"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOU30469.1"
                     /translation="MSYLFTSESVSEGHPDKIADQISDALLDEFLKWDPDSKVACETF
                     VTTGLVVCGGEVRTEGWVDVQETVRRVLREIGYTKAEYRFDCDSCGVISTIHEQSPDI
                     RQGVVKEKPEEQGAGDQGMMFGYACREMDNFMPMSIQLAHMLLKELAAIRKEGKVMTY
                     LRPDSKSQVTIEYEDNGEPLRIDTIILSTQHDEFVLPKNKSKEAVKEAEKAMQTQIKN
                     DILNILIPRVKKQLPERVSKLFKGDFKLYVNPTGKFIIGGPHGDTGLTGRKIIVDTYG
                     GHGAHGGGAFSGKDSSKVDRSAAYAARHIAKNMVAAGVCDK"
BASE COUNT         2753 a         1969 c         1738 g         2656 t
ORIGIN      
        1 taaaaaggtt tacatattgt ttcatccccg attttccggg cgaaattaat ctctcgggac
       61 gcctctccca ctggtgccgc ctacctgaaa atgcaaggtt tataggcatc ctttcccgtt
      121 ttcaatcgtt cccggagcct ctctctccct ctataaaact tttacccacc gatatttcaa
      181 gaagagaaga ggatatatca tatatttact gcacagctat aatgtcgggc ccatcacccc
      241 agaaggagct gctggcatca aaaattaaag atatactcct gaaaaccggc aagaactctg
      301 taatactgtc ggggaacatc tcatccgaaa gcatcataca gaaagaagac aatattcaaa
      361 ttataaatca tctgccagaa gatgaatttg cagagattgt tggcagaagt gaaattatta
      421 tttcccgttc gggatataca acagtgatgg agcttttcag cctgggccgg agtgctatac
      481 tgattcccac ccccggtcag cctgaacagg agtacctggc agaatacctc tccggcaaaa
      541 ggtggtttat acacttaaac cagagaaact taagcactaa tacaatgttt gaacttcctg
      601 atcttactct cccgaaattt aatgcatcgg aaaatgagaa attattaaat gctgccctgg
      661 aggaattact gaaggaataa cattgctgct gacacaactg aaaatcctac aaggaaccct
      721 gaatagacct cctgagggtt atgagcattg agctttagcc ttgccgccag aatcatacct
      781 gaaattagca gaaccggaac cagaaaccat acaaggtttt ctttcattac tatccacagt
      841 acagttacaa gtgcagtaag tgcgcctgca ccagctgaat gaactgaaat tttatatctg
      901 aaattgatca aaaagattat caatgacaat atggatactg actcaaaata tgcttttata
      961 aacaatggaa tactgagtcc aataataacg aatgaagtga taaaatataa aattgatacc
     1021 gtcaacaggg gaaaaatcct ttcggaattt ttttccattg tccatgaact aatatagttt
     1081 ctgtacctga aaaccagaat cattcctagc ggtagcaata cattgtttat caataaaata
     1141 agcagaataa ttttttttat accacctgga agataaaata gcagtgtagg tgcagtaaaa
     1201 attatcagaa ggccatacgc tggcataaaa agaggatgga aaagaattgt gatggctttg
     1261 gcagtgtaaa gttgaaattt ttctgaaaaa atatctctca ttatatttcc tttcgtaatc
     1321 gtgcaaccgg aatatttaac tgttccctgt atttggcaac ggtgcgacgt gcgatttgat
     1381 aacctttctg ctgaagtatc tcggttaatt tttcgtcagt aagaggtctt cttttatctt
     1441 cattttcaat acattcctga agtattttct ttatctcgcg cgttgacacc tcctcgcccg
     1501 aatcggtctg aagaccttca gaaaagaaat atttcagagg gaatatgcca aaatgagtct
     1561 gaatgtattt gctgctggca actcttgaaa ctgtagaaat atcgagtccg gtcatttcag
     1621 ctatgtcttt aagaatcatg ggtttaagtt tcgtttcatc accttcttta aaatattctt
     1681 cctgatattt aagaattgca ttcattgtaa gcagcagcgt gttctggcgt tgttttatag
     1741 catctataaa ccattttgct gaatctattt tctgctttac aaacataatt gcatccttca
     1801 tttctttttt ctttcccgaa tcgcgtgtat aatttctgag catttcagtg taggctttat
     1861 taagcctcaa ttcgggcagg ttgcgtgaat taaggtgaag ttcaaaaccg ttgtcagtaa
     1921 cttcaagtat gaaatcaggt ataatctgtt gtgcggtttc tgaagatgac tcactcataa
     1981 gactacccgg tttcgggcta agcttcatga tttcctcgat ggctttttta agttcatctt
     2041 ctccgacgcc aagtttcgac tcaattttgt cgtagtgttt tctggtaaac tcctcaaaat
     2101 ggtcttcaag aattttcttt gcgagtctta ccgaaggatt tgactggtct tttcttttta
     2161 tctgaagaat gaggcattcc tggaggtttc tggcgcccac acccgggggg tcaaggtcct
     2221 gcaccacttc aagaacctct tttacttcat tttccgtcgc attaacattc tgaagaaatg
     2281 ccagatcatc aacaatatta gaaatatctc ttctcaggta gccatcttca tcaatgttcc
     2341 cgattataaa ttcccctatt gttttttgct tttctgtcaa ttccctcagt ccgagctgtg
     2401 tagccaggtt ttcatgcagg gttgtaccta ttgagaaagg aatttccgga cgtttttcat
     2461 catccctgct gtagttcttt gcctgcagcc tgtattccgg aatatcttca tcgtcgtcga
     2521 tataatcatc gagggtaaat tcttcctgtt cctgttcatt gtcttcaaaa tcatcccctt
     2581 ccagttcttt cattattgtt tcctcatcat cttcctcagg gccctcctcc agtgcagggt
     2641 tctcctcaag ctctttcttt atcctctgct ccatctgaac agtaggtatc tccagcagtt
     2701 ttatcatctg tatctgctgg ggtgaaagtt tctggagaag tttctgctct aacctttgtt
     2761 ttagcatctt actgtgaaat ttacttgccc ggatgattaa aattcagcat tccttggtgt
     2821 tctcgggaaa ggtattacat ctcttatatt cgtcatgcca gtaaggaaaa taagaaatct
     2881 ctcaagcccc agaccaaatc cgctatgtgg agctgagcca aaccttctgg tgtcgatata
     2941 ccaccacaga tccttctcat tcatattcag ttctctgatg cgattgagta atttattgta
     3001 gtcttcctct cgctgtgaac ctcctattac ctcccctata ccggggaaga gaacatccat
     3061 agccttcacg gttttaccgt cgtcattcag tttcatatag aaggctttga tttcttttgg
     3121 gtagtctgta ataataacag gccgtttgaa gtattcttca accaggtatc tttcatgttc
     3181 tgcctgaagg tcgcgacccc actcaaccgg aaactcccat ttacgtcctg atgctttcaa
     3241 tatttcgata gcattggtat aagtgattcg ttcaaagggg tttttaagaa taaattcaag
     3301 gcgttcaatc aggcctttat caaacatatt gtttagaaac tcgaggtcgt ccctgcagtt
     3361 ttcaagagca tacctggtca ggtatttaag aaaatcctcg gccaggtcca tgttgtcgtt
     3421 tatgtcataa aaagccattt ccggttcaat catccagaat tcggcaaggt gcctcggagt
     3481 gttggaattt tcagctctga acgttggacc aaacgtatat atttcaccca gtgcaagtgc
     3541 accaagttca ccttcgagct gaccggatac agtaagattt gtttctttcc cgaaaaaatc
     3601 cttgctgaaa tcgactttac cttcctgatt ccttggaata ttgtcaaaat ctaacgtcgt
     3661 aacacgaaac atctcgccgg ctccttcaca gtcactgcct gtaattattg gtgtatgcag
     3721 gtaataaaaa cccttgtcgt tgaaatattt atgaattgca aacgcaatag catgccttat
     3781 tctgaagatt gcaccaaaag tatttgttct gaaacgcagg tgtgctattt ccctgagaaa
     3841 ctcaagagaa tgacccttct tctgaagagg gtatatttca ggatctgctt taccataaag
     3901 ctctatttct tctgctataa tttcaacagg ctgttctttc cctttcgatt ctgccagctt
     3961 ccccttaaca gctattgcag ccccggtatt aatatcctta agtagttgct caccgaattt
     4021 tactccttcg gcaacaattt gaatattttt tatcgtcgag ccgtcattaa gtgcaataaa
     4081 cactatttct ttaatgctcc tttttgttct tacccatcct tttacaagca cttcctcacc
     4141 taccgggaat cctgtaagaa gttcttttac cttagtcctt ttcatcagtt ttatataact
     4201 cttaaaattc agactccaaa aatagcaatt cagaaaatac aattgcactt tttgaaacaa
     4261 aactggcatt atttttgctc aaatacccgc attttatatt ttttctccag cgtttacaaa
     4321 atatttttaa actacttctg tttattaatc agaattattt attttcagct cccttgcagt
     4381 aaagtttgat taattgctga tttctgcgta attttacgat agaaaattaa ttatgaacac
     4441 tatggaacaa aatcagaagc ttattgttgt gccatgggat tttacgcatg ttgctgaaca
     4501 tgcactggct catgctgtga agatcagccg gatggtttca aaccagatat gtcttcttca
     4561 cattgtggat cccaaaatca agccagccga agagaaggct aaaagtgaaa gtctcaagca
     4621 tatagctgaa gaaaacagca aaaaatacaa tgtgcccatt acctctcata ttgagaaggg
     4681 aaccattttc aaatccattg gcgctttcgt aaatgataca gaaaaacgcg atgtaagcct
     4741 tgtggttatg ggcacccatg ggattaaagg gatgcaaaaa cttaccggaa gctgggctct
     4801 gaaagttatt gtcaaatcaa aagtgccttt tattgttgtt caggatcccc cgcgcgactg
     4861 ggaaagatac cataatattg tttgtccgat ggatttcagg tcggagaata aggaaaagat
     4921 taaaatggca atcttcatgg ggaaatactt cgattcaaaa atccatataa tcaaaaccac
     4981 ccctaccgat aagatgctga taaagaagac aaatatcaat ctcaactttg ctataaaatt
     5041 cttgattcag aacaatatag aatatgaaat tcacgacctg ccgaaaggtg attttgctca
     5101 gcagacgatt gattttgcgc agaaaataaa tgccgacctg atattgatag taacgactaa
     5161 aaatatcacc tttaccgatt acatgcttgg ggcctcagag cagtacataa ttgcaaatag
     5221 ttcaaaaata ccggtaattt gcgttaaccc gagggcatca tatgcaaaaa gctcccagtt
     5281 tatgggaggc tggtccgact gagttactta tccttatgtt gaggcatcag cagaacagaa
     5341 attactgcag caaccaggca tgcaataaat gtcgttagcc gtgcaggaac actttttacc
     5401 gtaaaagccg aactaacaac agcaattttc ttttcttcgc tgctccagcc tgtttgttct
     5461 cttattttat ctgccaggcg ggcagaattg acgaacttcc tgccttccag aacaagcaat
     5521 tgctctttct catggttact tattatattc tgactggcaa gaaaatcagc tttctctgac
     5581 gtaaccctgt aatgctgttc aaacccacca ctaacccaga atattgtaaa aagtattccg
     5641 gtaacaattg tagcaacagc tgccttccca tggaatcttt tccagaaaag cgtaagcaga
     5701 ataactaccg aaaaagtgca tccgattcct gcccacacat aacttaccag agtaaagata
     5761 atttttgaag gagaaacata tgagagcaaa agcgcaataa ctgctattat tcctgttatc
     5821 agccggtgct ttctcagcac cgctggtgat gtgtcctggt gtttttctcc ttttttcaga
     5881 aggcttccgg aaagttcagt tgccgaaaga ataagtagtg aatcggcagt tgaaatcatt
     5941 gcagctatgg cacctgtaat aagaatggct gctattaccg gagggaacaa tttaagaata
     6001 acgctgggca tcacatattc aggatcacca aggccagcgg gaccgaaaat agcaatacca
     6061 atccatccta tcattaatgc tcccgtgtaa gctacaatag tccagacaat accaacattt
     6121 cttccctttt ttgcctgttc cctgttgcgg attgccataa acctcattgt cagttgcggc
     6181 aatcctccga ggtatccgaa aaaccagctg aagccgcctg taataagcaa tccagctcca
     6241 aatccttttg caacacctgt cagagaagta tatgattcac ccgattttct tagagcttca
     6301 cctatccctg aggcataaag ttcaggatgg tttgaaatat aaattattcc cgccaccgga
     6361 gctataacca gtgctgtaat catcagaagt gcctgaataa catcagtata gaccacactt
     6421 ctgaaaccac catatattgt gtaggggatg ataatcaaag ccgtaataag catccccaat
     6481 cctggttcta ttccgaaaag agtatgcagt gtctttcctc ctccaagaaa ctgggcgccc
     6541 acataaaaaa agaaaaataa aactatcgtt aaactgctga ttaccctcaa cagtcggcca
     6601 gtgtcaccat gtctggctgc aagataatcg ctgaaagtcg tgacattata tttctcagct
     6661 tcatctctaa gtctccatgc cagcagcgcc caggcagtaa ttataccagc aacacaacca
     6721 attgcagtcc atatttccgt cagtcctgtt gcatatgcag caccaggcag cccgagaagt
     6781 gcccacgatg actcacctgt cgccctttcc gacagagctg ccacccagcc cggaagtttg
     6841 cgaccagcaa tagaataatc actgcccgtc tttaccttac gaccctgata aataccccat
     6901 aaaatcagca gggaaagata tgcaatgaga acaattacta tctgaagctg attttccata
     6961 acctctgaaa atttttacac atccaaaaat aatcagtaaa actatgataa aaaaccattt
     7021 tgataacaca tacttaaaaa ctatcttaga gccctgctca ggataaaacc atcgggtctt
     7081 atgtcaatta cagttgaaag ggttacaaaa tattacggca cccagaaagc tcttgataac
     7141 ttatcgtttg aggtaaagac aggggagata gtaggttttc tggggcctaa cggagccggt
     7201 aagtcaacaa tgatgaaaat tatcaccggc tttatcccac catcatcggg cagagttctg
     7261 ctcaacggca ttgaaacagg ctgcgacgat cctgaaatca ggcgtaaaat cggatatctt
     7321 cccgaaaata accctcttta tcctgagatg tatgtaagag agtatcttgg ttttgttgct
     7381 tcattctacc tttcgggcaa agagaggcag aaagccgtcg atgaaataat tgaacttacc
     7441 ggacttgttc ccgaacaaaa caagaagata ggctcattgt cgaaaggtta ccggcaaagg
     7501 gtcggacttg cgcaggcact tatccacaat cctgaaatac ttatcctcga cgaagcaact
     7561 tcaggactgg atcccaacca gatagtagaa atacgctcac tcattaaaga agctggaaaa
     7621 gaaaaaacag tgctcatttc aacacatata atgcaggaag ttgaagctat ctgcagcaga
     7681 ataattatca tcgacagagg aacaatagta gctgatgacc cgaaagaaaa gatttattca
     7741 agaattatgc aggcaataca gacagtgaaa gttgaattcg acagacaacc cgatgaaaaa
     7801 gaacttgcca gcatacccgg tgtaatttca gtaagcagaa cagggagcaa tatgtttata
     7861 attgaggctg aatcaaaagc cgatatacgt cctcatatct tttcatatgc agtaaataac
     7921 aacctcaccg tactctctct tataaggcag gagagcaatc ttgaagaagt ttttcgtaag
     7981 cttactgcat aatttttttg ttaatcataa atatttttat ttgctttgtg ctttcaaaaa
     8041 atggtggaaa gatttgtgct gattgcaacc acggaaaaaa atgctaaaat gcgaatattc
     8101 cttgcttttc agtcaacaaa aacttcccct tgtttgtttt aacctttaat aagtaaaata
     8161 ctaaatgagt tatttattca cttcagaatc agtttcagag ggtcatcccg ataaaatagc
     8221 cgatcagata tccgatgccc ttcttgatga gtttttaaaa tgggacccgg attcaaaggt
     8281 tgcctgcgaa acatttgtaa ctaccggtct ggttgtttgt ggcggtgaag tacgaactga
     8341 aggatgggtt gatgttcagg aaactgtcag aagagtttta agggaaatag gctataccaa
     8401 agccgaatac cggttcgatt gtgattcctg cggagtaatc tccaccatac acgaacagtc
     8461 gcccgatatc agacagggcg tggtaaaaga aaagcctgaa gaacagggtg ccggcgacca
     8521 gggtatgatg ttcggttatg catgccgcga aatggataat ttcatgccaa tgagtatcca
     8581 gctggcacac atgcttctga aggaactggc agccataaga aaagagggta aagtgatgac
     8641 ctatctgaga cccgacagta aatcacaggt tacaattgaa tatgaagata acggggaacc
     8701 cctcagaatt gatacaataa tattaagcac ccagcacgat gaatttgttc tgccaaagaa
     8761 taaatcaaaa gaggctgtga aagaagctga aaaagcaatg cagactcaaa tcaaaaatga
     8821 tattctgaat atcttaatcc cgagggttaa gaagcagctg cctgaaagag taagcaaact
     8881 ttttaagggc gatttcaaac tttatgtaaa ccccacagga aaatttatta taggtggacc
     8941 tcacggcgac acaggcctca caggccgtaa aattattgtc gacacctacg gagggcatgg
     9001 agcacatggc ggtggtgcat tctcaggtaa agattcatcc aaggtcgacc gttcagcagc
     9061 atacgcagca agacacatcg caaaaaatat ggttgccgca ggtgtgtgcg acaaag
//
LOCUS       DAOSNB010000068         9077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7624_length_9077_cov_3.779539, whole genome shotgun sequence.
ACCESSION   DAOSNB010000068 DAOSNB010000000
VERSION     DAOSNB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_9077_cov_3.779539"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PLG42_04315"
     CDS             <1..943
                     /locus_tag="PLG42_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOU30470.1"
                     /translation="KLKFQHCVAILGLLFLLDMFPVDKRYLNAGKFTTKSSALRFTLP
                     SAASQHILKDTTIFRVLNLTVSPFNDASTSLFHHSIGGYHGAKLKRYNELIDSVLYPE
                     IVNFTNMLQQPNPYPHIDSALQKLHGLNMLNTKYFIVNPDMPPLSNPYAIGNAWFIDK
                     IIFAENANEELASLKHINPSKEAVIDVRFKQYMPDASALKGSPGDTIRLVSYKPNELI
                     YKSVTTHERLAVLSEIYYPAGWIASIDGKEAPIIRANYILRALVVPPGNHEIILRFRP
                     ASYYTGEKISLASSIIYIILVIGYLFFNLLKKRQVDK"
     gene            940..1860
                     /locus_tag="PLG42_04320"
     CDS             940..1860
                     /locus_tag="PLG42_04320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU30471.1"
                     /translation="MKITYNKCPVCNSNSIGPYIECKDYLVSGESFPIYRCPECRLEF
                     TQYHPGEQESGRYYESVDYIPHSDAIKSFTGKIYYTVRKFMLLKKKNLVIKYTGKRTG
                     TILDFGSGTGHFAITMKKAGWDATGIEPDNDAREYSVKNFGIKAMRPEEMKILPSFSF
                     DCITLWHVLEHLHNPFQSLSEIKRLLRPNGILIIATPNNMSFDSIHYRHLWAAYDAPR
                     HLWHFNPVSFSYMAGKAGFEIISMKKLPFDVFYISILSEKNRGSRIPLFNGLLKGSIF
                     FLLSLFDVTRSSSIVYLLKHSESGKGNADY"
     gene            complement(1926..2906)
                     /locus_tag="PLG42_04325"
     CDS             complement(1926..2906)
                     /locus_tag="PLG42_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU30472.1"
                     /translation="MKRILIIQVIILTALLKVDGQQLPLYSQYLYNKFLIDPAVAGSD
                     GYTSFNLTAREQWVGYYGAPRTYSFSWQTRFLKRGHIIKQVSPRRQVFRPKSDGKVGV
                     GGFIFSDKNGLVRRTGFQATYAYHTWVQQATQLSMGLAVTGYHFKIDERDLNFEDPDE
                     PWLNNELRKGVFIPDASFGLYILNSRFSFGFSADQLFGARAKIGNDAYKKLQVSRHYY
                     LFGSYTFTDMRYHEFQPSFLLKMSEQFMPQVDVGFTYTYNQSVWGGLAYRTNNSIIAH
                     IGAKYQNIWIGYAFDFSMQEIQRLTYGSHELSVALKFGDSTRRYRWLDRY"
     gene            complement(2926..7482)
                     /locus_tag="PLG42_04330"
     CDS             complement(2926..7482)
                     /locus_tag="PLG42_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU30473.1"
                     /translation="MRRQNKISILSAGKSSVRITLLACAQVLINSSILLSQVITNNGA
                     AINITNGIVVNSKDAINTAGLLINSGELNLSGNFTNTAVTDGSSGIFRVGGNWTNTGG
                     VFQPGTSTVIFNGSGIQSIIRTGGETFYNMSLYNTGSPGSDRLLLPNNVTVQNLLTLS
                     SGNILTGTNKLLLSNPAADALVYTSSTESRIVGRFERGVNQTAKYLFPLGTLANYNPA
                     NITFNNITSAGTVLSEFLTPASIDSAGLPLPDPPDEVARVYQDGLWNFISNSFSSSDF
                     SIILDAKGFTTFPIQDITRIIKRTTGGSWTLDGTHSPATGTTVYRNNLTGDISPLGTQ
                     FTLAQSRPRIIQHPKDTIVCERSPATFTIIATSTRPLTYRWYKEPGILLTGPRYSVAS
                     DGTLVIVNAVLSDSGYYYCIVTDDYGNFTRSNSAHLGVNKRPIATVTPSSQDHECSNV
                     PFTNMIFGETAGVPGTTYLWTRTEPAGITTTLPSSGTVLNIGDFIGGTFINETDAPIT
                     ITFTIVPVGPSPTFCTGETIYATITVNPTPRVIPVSVKDEICDSRNANSALNNTHITL
                     TSPTVTTKGVVRFDYTVALSGLPGNLVGNTTPATDLPPGHVINFTYTNYTDTIQTVTY
                     SITPKVNGLSCPAGPVVDHVIKVHALPLPRITPPPSIDVLQPMTCTSGSGLAALRAVI
                     SEGADPYKIIWRGPVGYYMEDSVEIRNLFSGQYTVKVTDNIGCMREDYINIVPQLARG
                     YMVPTPIDPGGYPISCIGSSDGTVTISATGGITPPYNYWLVRNGVDTISTGVFSDNRN
                     PADPTTYRIITGLSAGTYTLYIRDKNGCFDENPKTVTLRPPPPVVVQFGTSNYAGGYN
                     ISCKGYNDGAAWVSSISGGRGGYHYRWFTYDGNIPGPDSTYRIDNLIAGTYYLEVTDT
                     LGCITIFNVTLTEPPGIELSGYRLSSSRDSSYNISCNGFSDGSIDITVTGGSGTYNFS
                     WTGPDGFTANTEDLKNLKAGTYSATITDVSNTTCILMPLPVFTLTEPAPLNVNAVPSV
                     SPDGNHNINCSGGTGSIDLTVSGGSPGNYRFNWSTDNGSGIVQGMEDQFALTAGKYNV
                     VVTDSNLCVATTEIVLTEPEPLTISLVPKHITCETPGFSNGSIDLTVTGGVPPYSYQW
                     SNGATTEDISNLSEGYYSVTVTDVNGCRITDSIRINLPPAVEFNSILSDYNGYNVSCT
                     GYSNGYINIEPTGGEAPFTYLWQGPDGFSSNERNISGLKAGQYQLIVTDANLCTATET
                     FVLNEPEKISLDLTLSRSNDGNYNINCAGDTTGSISVEAVNFVGGVSFLWPDGYIGKT
                     RTGLKAGTYRLIAIDRNGCHADTSVTLTQPDTIRILFSVTPPFCPDSPDGEISIEVHG
                     GIEAGGYMYRWSDNSTGQSLTNLTRGVYRVKVMDANNCTVTDSVRLETLNESCLVIPN
                     AFSPNNDNINDEWNIGMKHLYPQMEVKVFNRWGELVWKSEKGYPQPWDGRSNGVLLPM
                     DSYHYIIDLHNGKKSIIGNVTIVR"
     gene            complement(7542..8237)
                     /locus_tag="PLG42_04335"
     CDS             complement(7542..8237)
                     /locus_tag="PLG42_04335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOU30474.1"
                     /translation="MKKGIIILLILIPLILSAQKSKTKEPVKQSAGIITLSNAVDSSQ
                     YILGAYIGQYLRANNLVVTNPALFNKGLDDVLSGKPLLVAADTIPKIVNAYLSQAVIE
                     RNKVLEKQLFDAVKSQPGVGVLPSGVCYVIARAGEGARPLVADTVTIHIKGYLPDGTI
                     FEDTYTRNTPLKARPENLIPGLKEAVQIMQAGSVWRVYIPSSLAYGEKGIPGIIPPFS
                     ALVFDVELLSINK"
     gene            complement(8294..8464)
                     /locus_tag="PLG42_04340"
     CDS             complement(8294..8464)
                     /locus_tag="PLG42_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30475.1"
                     /translation="MKFRTSLFRVVNNCNLDAAELEFIIPDQFWYTGINEKFQDNLVL
                     HLFHLAGGTNSD"
     gene            complement(8558..>9077)
                     /locus_tag="PLG42_04345"
     CDS             complement(8558..>9077)
                     /locus_tag="PLG42_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30476.1"
                     /translation="TPIFRVDGVNGNTWLAPSTGKVGIGITAGTQPTALLHLNAGSSS
                     ANNAPLKFTAGTILTTAEDGAVEYDGTNFYVTNNSNIRYTIAKTLTVTTDLDFPNTPA
                     RTSNDLTVTVTGASLGDVVALGIFNSAVLANSTYTAWVSAANTVTVRFNNYSNGALNP
                     PAGTFRVSVIKY"
BASE COUNT         2615 a         1928 c         1747 g         2787 t
ORIGIN      
        1 caaactgaag tttcaacatt gtgtggcaat acttggcctg ctgtttcttc ttgatatgtt
       61 tccggttgac aaacgatatc ttaatgccgg aaaatttacc acaaagtcat cagctctccg
      121 gtttaccctt ccttctgcag ccagccagca tatacttaaa gatacaacaa tattcagggt
      181 tcttaatctc acagtatcac catttaacga tgcctcaact tctcttttcc atcactcaat
      241 aggtggctat cacggtgcaa aactgaaaag atacaatgag ctgatagatt cagttttata
      301 tcctgaaatt gtgaatttta caaacatgct tcagcaacca aatccctacc cccatataga
      361 ttctgccctg cagaagcttc acggacttaa tatgctgaat actaaatatt ttattgtaaa
      421 tcccgatatg ccaccccttt ccaatcccta tgccattgga aatgcatggt ttatcgataa
      481 aattattttc gctgagaatg caaacgaaga gctggcttca ctgaaacata tcaatccttc
      541 aaaagaagct gtaattgatg tccgcttcaa acaatatatg cctgatgctt cagcgctaaa
      601 gggttcacct ggcgatacca tcaggctggt ctcttataaa cccaatgaac tgatttataa
      661 aagtgtaaca acccatgaac ggctggcagt tttatctgaa atatattacc cggcaggctg
      721 gatagccagc atcgatggca aagaagcacc tattatccgt gccaactaca ttctgagagc
      781 tcttgtagta ccccctggaa atcatgaaat aatactccga ttccgacctg catcgtatta
      841 tacaggtgaa aaaatttcac ttgccagctc aataatatat attattctag taattgggta
      901 tctatttttc aaccttctta aaaaaaggca ggttgacaaa tgaaaattac ttacaataaa
      961 tgccctgtat gcaatagcaa tagcatcgga ccatatatcg aatgcaagga ctacctggta
     1021 tcaggtgaat cgtttcccat ttacaggtgt ccggaatgtc ggcttgaatt cactcagtat
     1081 caccctggcg aacaggaatc gggtagatat tatgaatcag tcgattatat cccccattcc
     1141 gatgcaataa aatcctttac aggaaagatt tattacactg tgaggaaatt tatgcttttg
     1201 aagaaaaaaa atcttgtaat taaatataca gggaaacgca ctggcaccat tcttgatttc
     1261 ggtagcggga ctggacattt tgcgataact atgaaaaagg caggatggga tgctacaggt
     1321 attgaacctg acaatgatgc cagagaatat tctgtaaaaa acttcggaat aaaagctatg
     1381 cgacctgaag aaatgaagat acttccttca ttttcatttg attgtataac tttatggcac
     1441 gtactcgaac atcttcacaa tccttttcag tcactctccg aaatcaaacg ccttctgagg
     1501 cctaatggca ttttgattat tgccacacct aacaatatgt cattcgacag catccattac
     1561 aggcatctat gggcagcgta cgatgcacca agacatctgt ggcatttcaa ccccgtttcc
     1621 ttttcctaca tggctggaaa ggcagggttt gaaataattt ctatgaaaaa acttcctttt
     1681 gatgtttttt atatatctat cctgagtgaa aagaatagag gttcgcgaat acccctattt
     1741 aatggccttt taaaaggatc tatcttcttt cttttatcct tattcgatgt tacacgaagc
     1801 agctcaatcg tatacttgct aaagcactct gaatccggaa agggtaatgc agattattaa
     1861 cacattcaaa cattgaataa cctattaccc attacataat acagtttgac gattcccaaa
     1921 aaacttcagt acctgtcgag ccatctgtaa cgtcttgtac tgtcgccaaa tttaagggca
     1981 accgataact cgtgtgatcc gtaagttaaa cgctgtatct cctgcatcga gaaatcgaaa
     2041 gcatatccga tccatatatt ttgatatttg gctccaatat gggcaataat tgaattattt
     2101 gttcggtagg cgagacctcc ccacaccgac tggttatatg tataagtaaa ccccacatct
     2161 acttgaggca taaattgttc cgacattttt agaagaaatg atggctggaa ctcatgatac
     2221 ctcatgtctg taaaagtata tgagccaaac agataataat gcctgctcac ctgcagcttt
     2281 ttatatgcat catttccaat tttagccctt gctccaaata actggtcggc agaaaaaccg
     2341 aagctgaagc gagaattcag aatatagagt ccaaatgaag cgtcagggat aaacactcct
     2401 tttctaagct cattattcag ccatggttca tcagggtcct caaaattgag atctctttca
     2461 tcaatcttga aatgatatcc ggtaaccgca agacccattg acaattgtgt cgcctgttga
     2521 acccaggtat gatatgcata agttgcctga aaaccggtac gtctcacaag accgttttta
     2581 tcactgaaaa taaatcctcc aactccgacc ttcccgtctg attttggtct gaaaacctgc
     2641 ctccttggac ttacctgttt aataatatgt cctcttttta gaaatctggt ctgccagctg
     2701 aatgagtacg tacggggggc accataatat ccgacccact gctcccttgc agttagatta
     2761 aaactagtat aaccgtcgct accagcaact gcaggatcaa tcagaaactt gttgtagaga
     2821 tactggctgt acaaaggcag ctgctggccg tcgaccttaa gcagagcagt caaaataatt
     2881 acctgtatta tcagtattct cttcaaagtt ttataaagtt aatgtttatc tgacaattgt
     2941 tacatttcct attatcgatt ttttgccatt atgcaggtca attatataat ggtacgaatc
     3001 catcggtaaa agtactccgt tgcttctgcc atcccatggc tgaggatatc ctttttctga
     3061 cttccataca agctcacccc agcggttaaa tactttcacc tccatctgag gatacagatg
     3121 cttcatgcct atattccact catcattaat attatcatta tttggtgaga atgcatttgg
     3181 aataaccaga cacgattcat taagtgtttc aagccttact gagtcggtca ccgtacaatt
     3241 attagcatcc atcactttca ctctatacac ccctctcgtc aggttagtca aagattgtcc
     3301 ggttgagttg tcagaccatc tgtacatgta gccaccagct tcaattccac catggacttc
     3361 aatacttatc tcaccatcgg ggctgtcggg gcagaatggg ggtgtaacgc tgaaaagaat
     3421 cctgatagtg tcgggctgag taagtgtaac tgaagtatca gcatggcacc cattccggtc
     3481 aattgcaatc agtcggtaag ttccggcttt aagtccggtt ctggttttgc ctatatatcc
     3541 atcgggccac agaaaactta caccacctac aaagttgaca gcctcaacac tgatggaacc
     3601 tgttgtatca cctgcacaat taatattata attaccatcg ttgcttcttg agagtgtaag
     3661 gtctaaactg attttttcgg gttcattcag aacaaaagtt tctgtagctg tacacaaatt
     3721 ggcatcagtt acaatcagct gatactgtcc ggccttaagg cctgaaatgt ttctttcatt
     3781 tgatgagaac ccatcaggtc cctgccataa atatgtaaac ggagcttccc caccggttgg
     3841 ctcaatatta atatatccgt tactgtaccc tgtgcagctt acattataac cattataatc
     3901 cgacagaata ctattaaatt cgacagcagg aggcagatta attctaattg aatcagtaat
     3961 acggcatcca ttgacatctg ttaccgtgac tgaataataa ccttcggaaa ggttgcttat
     4021 atcctcggtt gtagcgccgt tcgaccactg atatgaatat ggaggaactc cacctgttac
     4081 tgtaaggtct atagaaccat tgctaaatcc tggagtttca caggtaatat gtttcggaac
     4141 aagtgaaatt gtcagaggtt ctggttcagt aagaacaatt tctgtcgtgg caacacacag
     4201 gttagaatca gttacaacaa cattatattt tccagccgtc agggcgaact ggtcttccat
     4261 tccctgaaca attcctgaac cattgtcggt actccagtta aatctgtaat ttcctggact
     4321 tccgcctgac actgtcaaat ctattgaacc agttcctccg gaacagttaa tattatgatt
     4381 tccatctggt gaaactgatg ggacagcatt tacattaagc ggagcaggtt cggttagcgt
     4441 aaataccggc agaggcatta aaatacatgt agtattcgaa acatccgtta ttgtggctga
     4501 ataagttcct gccttcaaat tcttcaaatc ttcagtattt gctgtaaacc catctggacc
     4561 tgtccagctg aaattataag tacctgaacc gcctgtgact gtaatatcaa ttgacccatc
     4621 actgaaacca ttgcatgaaa tgttataact gctgtccctg cttgatgaaa gcctgtatcc
     4681 agaaagttca ataccgggtg gttcggtgag ggttacatta aatattgtaa tacatccgag
     4741 ggtatctgtt acctcgagat aatatgtacc tgcaataagg ttgtcaattc tgtaagtgct
     4801 atctggtccg ggaatgtttc cgtcatatgt aaaccatctg taatggtatc cgcctcttcc
     4861 tcccgaaata gagcttaccc atgcagcacc atcgttatat cctttacatg atatgttata
     4921 tcctcccgca taattgcttg ttccaaattg tactactaca ggtggcggag gtcttagggt
     4981 aacagtcttt ggattttcat caaagcatcc atttttgtct ctgatatata aagtatatgt
     5041 gcctgcggat agtcctgtaa taattctata tgtagtagga tcagcagggt ttctattgtc
     5101 ggaaaatact cctgtagaaa ttgtatcaac accgtttcta accagccagt aattatatgg
     5161 cggagtaata cctccagtgg cagaaatggt aacagttccg tcagaactgc cgatgcatga
     5221 tataggatag ccacccgggt ctattggtgt aggaaccata taacctcttg caagttgtgg
     5281 tacaatgttt atataatctt ctctcatgca ccctatattg tctgtaacct taactgtgta
     5341 ctgcccgctg aaaagatttc tgatttcaac agaatcttcc atataatacc caactggtcc
     5401 cctccatatt attttataag gatcggctcc ttcagaaatt accgccctga gtgcagcaag
     5461 acctgagcca cttgtgcagg tcatcggttg aagcacatca atactgggag gaggtgtaat
     5521 tcttggcaat ggcaatgcat gtaccttgat aacatggtca acaaccggac ctgcaggaca
     5581 ggagaggccg ttaacctttg gagtaataga atatgttaca gtttgtattg tatctgtata
     5641 gtttgtgtaa gtaaaattaa ttacatgccc cggaggaagg tcagtggctg gagtggtatt
     5701 tccaacaagg ttgccaggta atccgctcag agcaacagta taatcaaatc taactacccc
     5761 tttcgttgta acagtaggtg aagtaagggt tatatgtgta ttattcagag cgctgtttgc
     5821 attcctgctg tcgcatatct catctttaac gctcaccgga ataactcttg gtgttggatt
     5881 taccgttatt gttgcatata ttgtttcgcc agtacagaat gttggagaag gaccaacagg
     5941 cacaattgta aatgtaattg ttatcggtgc atctgtttca ttaataaatg tgccaccaat
     6001 aaaatctcca atgttcaaaa ccgtaccact cgacggtaat gttgtagtaa tcccagccgg
     6061 ctcggttcgt gtccacaggt aagttgttcc aggtacacct gctgtttccc cgaatatcat
     6121 gtttgtaaat ggaacattag agcattcatg atcctgagaa gacggtgtaa ctgttgctat
     6181 aggtctcttg ttaaccccaa gatgtgcaga attgcttctt gtgaaattac cataatcatc
     6241 tgttactata cagtaataat atcctgaatc agaaagtacg gcattaacaa taaccagagt
     6301 tccgtcactt gcaacactgt agcgcggacc tgtaaggaga attccgggtt ctttatacca
     6361 cctgtatgta agaggacgtg ttgatgttgc tattattgta aatgttgccg gagaacgttc
     6421 acatactatt gtatctttag gatgttgaat tatccttggc ctgctctgtg ccagtgtgaa
     6481 ctgtgtccca agaggtgaaa tatctcctgt aagattgttc cggtaaactg ttgtacctgt
     6541 tgccggactg tgagtaccgt cgagtgtcca gctcccgccc gttgtgcgtt ttattattct
     6601 tgttatatcc tgaatcggaa aagttgtgaa acctttagca tcgagtataa tactgaaatc
     6661 actgcttgag aaactgtttg atatgaagtt ccataggcca tcctggtaaa cccttgccac
     6721 ttcatctgga ggatcaggta atggaagacc tgcagaatca attgatgccg gagttagaaa
     6781 ttcagaaagg accgtaccgg cgctggttat attattgaat gtaatatttg ccggattgta
     6841 attggcaagt gtaccaagcg ggaaaagata ctttgctgtc tgatttactc ctctttcgaa
     6901 ccttccaact atacggcttt ccgtggagga agtgtaaacc agtgcatcag ctgcagggtt
     6961 cgacagaagc aatttattag tgccggtgag tatgttgccc gatgataatg taaggagatt
     7021 ctgtaccgtt acattattgg gcagtaaaag cctgtccgaa ccaggggaac cagtattgta
     7081 aagtgacata ttgtaaaaag tctccccgcc ggttcttatt attgactgaa tacccgaacc
     7141 gttaaatatt actgtgctgg tacctggctg gaatacacca ccggtgtttg tccagtttcc
     7201 gcctactctg aatataccgc tactgccatc cgtgacagct gtattagtga aatttccgga
     7261 aaggttgagt tcaccgctgt taatcagaag ccctgcagtg tttatggcgt cttttgaatt
     7321 tacaactatg ccgttagtta tatttattgc tgccccgtta tttgtgataa cctgtgagag
     7381 taatattgaa gaattaatta acacctgtgc acaagcaagc agggtaattc taacagagga
     7441 tttgcctgct gataaaattg atattttgtt ttgcctgcgc ataacttacc tcacctccct
     7501 gctccatccc tttgagaaga gagggaggga ggcagaatat tttatttgtt aatactcaat
     7561 aattcaacat caaacactaa ggccgaaaac ggtggtatga tacccggaat tcccttttca
     7621 ccataagcca gagatgatgg aatatatact ctccacacag aacctgcctg cattatttgt
     7681 actgcttctt tcaatcctgg tatgaggttt tcagggcggg ccttcagtgg tgtattacgg
     7741 gtataagtat cctcgaaaat tgttccatcg ggcagatagc ctttaatgtg tatcgtaacg
     7801 gtatctgcaa caagtggtct agctccttct cctgctctcg caatcacata acatacacca
     7861 ctcggtaata cgcctacacc gggttgactt tttacagcat caaataattg tttttccaaa
     7921 actttatttc gttctataac ggcctgactc aaataagcat taactatttt cggaatggta
     7981 tcggctgcaa ccagtaaagg ttttcccgac agaacatcat caagcccttt attgaaaagg
     8041 gcaggatttg ttactaccag attattagcc ctcaggtatt ggcctatata ggcacctaag
     8101 atgtattgtg aagagtcgac ggcattcgac agggtaataa ttccggcaga ctgtttaact
     8161 ggctctttag tttttgattt ttgggccgat aatattaatg gaatcaaaat caatagtata
     8221 attattcctt ttttcatttg cattaatatt ttaattatta ataaaatctc tcatgatact
     8281 gtaaattaag atactagtca gagttagttc cgcctgcaag gtgaaataga tgaagcacca
     8341 gattatcttg aaatttttca ttaattcctg tgtaccagaa ctgatcaggt ataataaatt
     8401 ctaactctgc agcatcgaga ttgcaattat tcacaacccg gaaaagtgaa gtcctgaatt
     8461 tcattattct gctttttttg ataatggcaa tcataccttt ttttattgat atactcaatt
     8521 ccaattattt tttgacattc ttagaatatt tttaacctca atattttata acacttactc
     8581 taaatgttcc tgcaggagga ttaagtgcac cgttgctata attattgaac cttacggtta
     8641 ctgtattggc tgcgcttacc catgcagtat atgttgaatt agctaatacg gcagaattaa
     8701 atattcccaa tgccacaaca tcacctagtg aagctcctgt tactgttact gtcaaatcat
     8761 tacttgtacg cgcaggagta ttcggaaagt caagatccgt agtaactgtc aaagttttag
     8821 caatagtata cctgatatta gaattattcg ttacataaaa attagtacca tcatattcta
     8881 ctgctccgtc ttctgctgtg gttaaaattg tccctgcagt aaattttaga ggtgcattat
     8941 ttgctgaaga tgaccctgca ttaaggtgca ggagagcagt tggttgagtt cctgcagtaa
     9001 ttcctatgcc aactttaccg gtcgatggtg caagccatgt attcccgtta acaccatcaa
     9061 ctctgaaaat tggtgtc
//
LOCUS       DAOSNB010000069         9037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7664_length_9037_cov_4.609552, whole genome shotgun sequence.
ACCESSION   DAOSNB010000069 DAOSNB010000000
VERSION     DAOSNB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7664_length_9037_cov_4.609552"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /locus_tag="PLG42_04350"
     CDS             <1..2283
                     /locus_tag="PLG42_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU30477.1"
                     /translation="IAFFLMTSGIIEAQSSLTIEKIMLGEKFTGISPESPRWSPSGNN
                     LYFTWTENVDSMPSLYVVREGNRIPQKVTLEERKNLPTEGNYNRKRTMYVYSKNGDIF
                     LLNLKTGNTLQITSTSTPETNPVFNLKEDKIIFVSGNNIFSWDINKGTFNQLTDFRSG
                     KEQATEPPYRNENEKWLYNSQLTLFKTLAERKRLREITLNESKAMAPVSPKTIFTGQG
                     TTVSVRMSPDEKYVTWIQTQRGEEKNTIVPNYVTESGYTGEIQSRPKVGTSQYSSKLY
                     IFDISRDTVYQAVSGDIPGLSEKRLITFRPPVWSDDGSRAVVEVLSADNKDRWIMLLD
                     AGTGKLKLLDNQHDSAWIGGPGTRSGPLGWLPDNKTIYFQSEESGYSHLYLLDVQTGE
                     KKAITSGNFEVYEPKLSNDGKYWYFISNETDPGIRELYRMPVRGGERTRLTSFGGGVE
                     YTLSPDEKYFALRVSFANRPWELYIMENKEKAIPVKITESLTPEFRAYNWRAPEYVRF
                     RASDGTMIPARLYRPASNVRNGAAVIFVHGAGYLQNAHRWWSSYFREYMFHNFLVDNG
                     YTVLDIDYRGSAGYGRDWRTAIYRYMGGKDLDDHIDGAKYLVEEHQIDPERIGIYGGS
                     YGGFITLMAMFTKPGVFAAGAALRPVTDWAHYNHGYTSNILNTPVADSIAYVRSSPIY
                     YAEGLIGELLICHGMIDDNVHFQDVVRLVQRLIELGKDNWELAVYPLESHSFREPSSW
                     TDEYKRIFKLFERTLNGKNK"
     gene            complement(2313..3350)
                     /locus_tag="PLG42_04355"
     CDS             complement(2313..3350)
                     /locus_tag="PLG42_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30478.1"
                     /translation="MKDAETVTLIRQGIGHIYNFKFDEAEKVLDQLKVSYPGHPVINL
                     YKGMMIYWQNFPLIPSSVKRSAFEQELYRSIELTDRNIISGEFEAEYLLANLSARGLL
                     LLFYADNGLSSEVIPLAAETYRPLMQSFRYTSECPDLYYFTGVYNYYRDAYPRVRPIY
                     KAVAFLFPQGNMQLGLQQLEICGKEAMALQAEAYSIISWIRMHFENDYQKSLPYCKLL
                     YEKYPDNVFFKSMYIKNLLLLKKYNEAEEILGIESETDTNSFYKALRSFFTGIIFEKK
                     YRNFGNAGKFYHEAIDALQPFGEYGKEYQAYAYFGLSRLGLKEDEKHQRRIYRRKALE
                     LSDFKKITFDD"
     gene            3623..5101
                     /locus_tag="PLG42_04360"
     CDS             3623..5101
                     /locus_tag="PLG42_04360"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOU30479.1"
                     /translation="MTLTADTIVLIISLLFFASILAGKISFRLGVPTLLLFLAVGMLA
                     GSEGIGHIRFDQPGIAQFIGIIALCFILFSGGFETDISYVKPVIMHTLLLSTLGVIIT
                     AVVTGLFIWKVTGFTLYEGLLLGSIISSTDTAAVFSILRSKKLGLKGHLRPVLEMESG
                     SNDPMAVMLIIMFISLVQDPSKSFWSVIPIFFRQLIIGGAFGVFFGWADRFIINKINL
                     HYEGLYPPLAITLMLMTYGLTSSLEGNGFLAVYISALWLGNQELLHKKTIMKMFDGLA
                     WLMQIILFLVLGLLVYPSRLLAIAVPGLLISLFLMFAARPIAVYLSLLPFRMKSREKI
                     FISWVGLRGAAPIVFATFTPVAGIEKADTIFNIVFFISITSVLIQGTSILKVAKWLHV
                     ALPARVKHVTPADILLAEPVNTAMAEITVSGESPSAGKKIVDLGFPENVRIALLKRDN
                     KYIIPDGQTYIKPEDKLILIAGNRETLNSVIDELCTQKVSEI"
     gene            5195..6202
                     /locus_tag="PLG42_04365"
     CDS             5195..6202
                     /locus_tag="PLG42_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30480.1"
                     /translation="MKLFKLLLVSIAFYGCVSQDGKDIIITVKGPVKAESTGTWLTHE
                     HILVDFIGADSINPGRYNRQDVTRKTLPFLLQARDLGCRTFVECTPEYLGRDPELLRT
                     LADSSGLNILTNTGFYGAVNNKYIPSFAFSLTAEELAGIWTGEWEKGIGQTGIKPGFI
                     KISVERDSLSEFHARLVKAAAITHLKTGLVIASHTGPSIPAFQQIELLEKEGVSPEAF
                     IWIHAQAEKDPEVLCRAASKGAWVSIDNLNENNVSDFVSILKAMKEKGCLNRVLISHD
                     AGWYDPAKENGGDFRGFTTMFEKLIPELKNNGFTDKEIKQVFEVNPARAFAVRIRRLK
                     N"
     gene            6213..7865
                     /locus_tag="PLG42_04370"
     CDS             6213..7865
                     /locus_tag="PLG42_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU30481.1"
                     /translation="MKYFYTLAGLLLLLSVLSCNHSSNKKAIRADIIIYGGTSAAVTA
                     AVQAAEMGHRAVIVSPDRHLGGMSSSGLGFTDTGNKEVIGGLARRFYQLIYEHYADSS
                     AWKWQKRSEYGNRGQGNPAIEGDKRTMWIFEPHVAEAAFEKMVSHPLIKVYRDEWLDR
                     EHGVEKKDGKIVSITTLSGKRFKGKVYIDVTYEGDLMAAAGVSYTVGREANSVYGEVW
                     NGVQKGVFHHGHYFKTNISPYWIPGDPSSGLLPRIYPGDPGENGSGDKKVQAYCFRLC
                     LTQHPENKVPITAPEGYDSTQYELLVRVAESQWKEFFAKYDPIPNWKTDVNNHGPFSY
                     DNIGMNWDYPEASYERRKEIIEEHIRYQKGLLYFTATCKRLPEWVRTEMNKWGYAKDE
                     FTDNGNWPYNIYVREARRMIGEYVTTEHDVLSRREVPDPVGMGSYTMDSHNVQRYVTP
                     EGFVQNEGDIGVRVPKPYKIAKGSLMPKREECLNLLVPVCVSSSHIAYGSIRMEPVFM
                     ILGQSAATLASLAIEKRKTIYEIPYEELKTELIKYGQILEYQ"
     gene            7916..>9037
                     /locus_tag="PLG42_04375"
     CDS             7916..>9037
                     /locus_tag="PLG42_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30482.1"
                     /translation="MKKKILFATLCLISLSLLPQEDPFPLIDLHVHIKEDFTIEKALQ
                     KSRAEHIQYGIAINCGVGFPVQSDRQVDSFLLEMKKYPEFFVGMQAEGREWVKLFSKE
                     AIAKFDYVFTDGMTFFDSKGRRNRIWLKEETWIEDEQKFMDYLVRTIVKILNEEPINI
                     YVNPTFLPEQMADRYDYFWTDKRMDAVIEAAKKNNIAIEINNRYRIPSEKFIRRAKAA
                     GVKFTIGTNNANSRFGRAEYALEMIRKCGLTESDFWKPVKKNRTVVTIIDDKFYINGQ
                     VTYPGRYWKGNRIEGLLMNSRMVQGIFDDLNPETARRWVYPDTRVWDPDRNTREFVLN
                     MKKWYDHGLLAFTINLQGGSPTGYSQEQPWHNSAYNADGS"
BASE COUNT         2790 a         1824 c         1985 g         2438 t
ORIGIN      
        1 attgcgtttt tccttatgac ttcaggcatt attgaagcac agtcgtcact gacgattgag
       61 aagataatgc tgggcgagaa atttacagga atctctcctg aatcgccccg gtggtcgcca
      121 tcgggaaata acctctactt cacatggact gaaaacgtcg attccatgcc ttcactctat
      181 gttgtgaggg aaggcaaccg gataccgcag aaagtaactc ttgaagagag aaaaaatctt
      241 cccacggagg gcaactataa ccgaaagaga acaatgtatg tttattcaaa aaacggagat
      301 attttcctgc ttaatctcaa aaccggaaat accctgcaga ttacctccac atccactcct
      361 gaaaccaatc cggttttcaa ccttaaagaa gataagataa tctttgtttc gggaaacaat
      421 attttttcgt gggatataaa caaagggacc tttaaccagc ttacagactt tcgatccggt
      481 aaggagcagg caactgaacc tccttacaga aatgaaaacg aaaaatggct ctataacagc
      541 cagctgactc ttttcaaaac acttgccgaa aggaaaaggt taagggaaat taccttgaat
      601 gaatcaaaag ccatggctcc tgtcagtccg aaaactattt tcaccggcca gggaacaaca
      661 gtatccgtca gaatgagtcc agatgagaaa tatgtcacct ggatccagac acaaaggggc
      721 gaagaaaaaa atacaatagt gccaaattat gtcacagagt caggatatac aggtgagata
      781 cagagcaggc caaaagtcgg aacttctcaa tattcttcga aactgtatat ttttgatatc
      841 agccgcgaca cggtatacca ggcagtaagc ggagatattc ccggactatc agaaaaacgc
      901 ttaataactt ttcgcccgcc tgtgtggtcg gatgatggca gccgtgctgt agtggaagtt
      961 ttatcggctg ataacaaaga ccgctggata atgcttctcg atgccggaac aggcaagctt
     1021 aaacttctgg ataaccagca cgattcagca tggataggcg gtccgggcac acgttcgggc
     1081 ccacttggct ggctacccga taacaaaaca atttatttcc aatcggagga atcgggctat
     1141 tcacaccttt accttcttga tgttcagaca ggtgaaaaaa aggcaatcac atcaggcaat
     1201 tttgaagttt acgaacctaa actttccaat gacggaaaat actggtattt catttccaat
     1261 gagacggacc ctggtataag agaactctac agaatgcctg tgcgtggagg cgaaagaaca
     1321 cgtcttacca gcttcggagg gggtgttgaa tacactcttt cacccgatga aaaatatttt
     1381 gcattaagag tttcatttgc taacaggcca tgggagcttt atataatgga gaacaaagag
     1441 aaagctattc ctgttaaaat caccgagtcg cttactcctg aattcagggc ttacaactgg
     1501 cgggcccctg aatatgtcag gttcagagca tcggatggaa caatgattcc ggcaagatta
     1561 tacaggccgg caagcaatgt tcgcaacggt gctgcagtaa tttttgtaca cggggcagga
     1621 tacctgcaga atgctcatag atggtggagc agctatttcc gtgaatatat gttccacaat
     1681 tttctggttg ataacggata taccgttctc gatattgact accgaggaag tgcaggatat
     1741 ggacgtgact ggcgaacagc catctacagg tacatgggag gaaaggatct cgacgaccat
     1801 attgacggag caaaatacct tgtagaagag catcaaatcg accctgaaag gattggtatc
     1861 tacggaggtt cttatggcgg tttcataaca cttatggcaa tgttcacaaa acccggagta
     1921 tttgcagcag gagcagcact ccgtcctgta accgactggg cacactataa ccatggctat
     1981 acctcaaaca ttcttaacac acctgttgcc gacagtattg catatgtaag gagttcacct
     2041 atttattacg ccgaaggact gattggggaa cttcttattt gccatggaat gattgacgat
     2101 aacgtccatt ttcaggatgt ggtaagattg gtacagcgtc ttattgaact tggaaaggat
     2161 aactgggaac tggcagttta cccgcttgag agccatagct tcagggaacc gtcgagctgg
     2221 acagatgaat acaaacgtat attcaaactg tttgaaagga ccttgaatgg aaagaataag
     2281 taaaaaacag gtgaatgact gaaatatata tctcagtcat cgaatgtaat ctttttaaag
     2341 tcggataact caagcgcctt tcgacggtat atcctgcgct gatgcttctc gtcttccttg
     2401 agtccgagac ggctcaatcc aaaatatgca taagcctggt actcttttcc atattcacca
     2461 aagggctgaa gggcatcgat agcttcatga taaaatttcc cggcattgcc gaagttcctg
     2521 tatttctttt caaaaataat tccggtaaag aaagagcgta gagctttgta aaatgaattt
     2581 gtgtcagttt ccgactctat tcccagaatc tcttctgcct cattgtactt tttcagcaga
     2641 agcagatttt tgatatacat gcttttaaag aaaacattat caggatactt ttcatacagt
     2701 agtttgcagt aagggagcga cttctggtaa tcattttcga aatgcattct tatccatgaa
     2761 atgatggaat atgcttcagc ctgaagagcc attgcctctt ttccgcatat ttcaagctgt
     2821 tgcaacccga gctgcatatt cccctgcgga aacagaaaag caactgcttt atatatgggc
     2881 cttacacgtg gataagcatc acggtaataa ttgtaaacgc ctgtaaaata atataaatct
     2941 ggacattcgg acgtatatct gaatgattgc atcaatggcc tgtaagtttc ggctgcaagt
     3001 ggaataactt cactgcttaa tccattatca gcataaaaaa gcaaaagcaa tcctcttgca
     3061 ctcagattgg caagaagata ttcggcttca aattcacctg aaataatatt tctgtcggtc
     3121 aattctatgc ttctgtacaa ttcctgttca aaagcagaac gtttaaccga tgagggaata
     3181 agcgggaaat tctgccagta tatcatcatc cctttataaa gattgattac aggatggcct
     3241 ggatatgaaa ccttcagctg atccagaact ttttccgcct cgtcgaactt gaaattataa
     3301 atatgcccta ttccctgcct tattagagtg acagtttcag catctttcaa aatctgaccc
     3361 ccggttttaa atgaaaaaat taaagtaagg aggaataaaa atatgacctg atttaatctg
     3421 ttgaagcgac tcctttgcat aactttttgg aaattaattc aaagcatcat cctcaagcca
     3481 acatatccag aaatgccatt gccaaattta aatgaaaagg gatgtaattt gatttcaata
     3541 aagataataa aatatctgcc tggaactaag tttttgatat catttatcta tttttggata
     3601 aacatggaaa ttagttatac ctgtgacatt aacagctgac actatagttc ttattatttc
     3661 attgttgttt tttgcaagca ttcttgcagg aaaaatttca tttcgtcttg gtgtacccac
     3721 actgctgctt tttcttgcgg tagggatgct ggcgggttca gaaggcatag gacatataag
     3781 attcgaccag cctggtattg cacagtttat aggaataata gcattatgct tcatactttt
     3841 ttcaggaggc tttgaaactg atatttcata cgttaaacct gtaataatgc atactcttct
     3901 cctttctaca ctcggagtta ttataacagc ggttgttaca ggtttattca tctggaaagt
     3961 aaccggtttc acgctttatg aaggccttct gctgggctca ataatatcat ccaccgatac
     4021 agctgcagta ttttcaatac ttcgctcaaa aaaacttgga cttaaagggc atctgcgtcc
     4081 tgtgctggaa atggaaagtg gcagcaacga ccctatggca gttatgctta ttataatgtt
     4141 tataagcctg gttcaggatc cttcaaaatc attctggtcg gtaatcccta tatttttccg
     4201 tcagctgatc attggaggag cctttggcgt atttttcgga tgggccgata gatttatcat
     4261 aaacaaaata aacctccatt atgaagggct ttatcctcct cttgcaatta cactgatgct
     4321 gatgacttac gggttgacct cctccctgga aggcaacggc tttctggctg tttatatatc
     4381 cgccctctgg cttggaaacc aggaactgct acataagaaa acaataatga aaatgttcga
     4441 cggactcgca tggctgatgc agattatttt gtttctggtg ctcggactcc tggtttatcc
     4501 cagtcgcctt ttagcaatag cagtaccagg cctgctgatt tcactctttc tgatgtttgc
     4561 agcacggcca attgcagtct atctgagcct tcttcccttt agaatgaaaa gcagggagaa
     4621 aatttttatt tcatgggtcg gactaagagg tgctgctcca atagttttcg ctacattcac
     4681 ccctgtggca ggtattgaaa aggctgatac gattttcaat atcgttttct ttatttctat
     4741 tacttcggta ctgatacagg gaacatccat tttaaaagtt gcaaaatggc ttcatgtggc
     4801 actgccggca agagttaaac atgttactcc ggctgatatt ctgcttgcag aaccggtgaa
     4861 cactgctatg gcagaaataa cagtttctgg cgaatcaccc tcagcaggca aaaaaattgt
     4921 agacctcgga ttcccggaaa atgtcagaat agccctcttg aaaagggata ataaatatat
     4981 catacccgac ggacaaacat atattaaacc cgaagataaa cttattttaa tagctggaaa
     5041 cagggaaact cttaattctg tgatagatga actttgcact caaaaagtat ctgagatata
     5101 gatgtttatc ttctttctac ggaaaaggca gatccatcct gactgtgcag gttgattttt
     5161 atttaatttt aaattcgtga ttattttaaa gtttatgaaa ctattcaaac ttctacttgt
     5221 ctcaatcgct ttttacggct gtgtatcaca ggacggaaag gatattatta taacagtaaa
     5281 aggtcctgtt aaagctgaaa gtacaggaac atggctgact catgaacata ttctcgtcga
     5341 ctttattggt gccgacagta tcaaccccgg gcgttataac cgtcaggatg ttacaagaaa
     5401 aacccttcca ttccttttgc aggcaaggga tctgggttgc agaactttcg ttgagtgcac
     5461 acctgaatac ctcggaagag atcctgaact gcttcgtaca ttagccgatt cttcggggct
     5521 caatatcctc acaaatacag gtttctatgg agccgtgaac aataaataca taccttcatt
     5581 cgcattctct cttactgctg aagaacttgc cggaatatgg acaggtgaat gggaaaaggg
     5641 tatcgggcaa accggcataa aacccggctt cataaaaata tcagtggaaa gggactccct
     5701 ttctgaattt catgcccggc ttgttaaggc tgctgctata acacatctga aaacaggact
     5761 ggttatcgca tcacacaccg gaccctccat tccggctttt cagcaaatcg aattgcttga
     5821 aaaagaggga gtctctcccg aggcttttat atggattcat gctcaggctg aaaaggatcc
     5881 tgaagtactc tgcagagctg catcgaaggg agcatgggta agtattgaca atctgaacga
     5941 gaacaacgtc agtgattttg tttccatatt gaaagcaatg aaagaaaaag gttgcctcaa
     6001 ccgggtgctg atatcacatg atgcgggatg gtacgaccct gcaaaggaga atggaggcga
     6061 tttcagaggg tttacaacaa tgtttgaaaa attgatccct gaattaaaaa acaacggttt
     6121 tacggataaa gaaataaaac aggtttttga agtgaaccct gcaagggcat tcgctgtaag
     6181 aatcagaaga ttaaaaaatt aagtatttgt ctatgaaata cttttatact ctagccggat
     6241 tattgcttct gctgtcagtt ttatcctgca atcacagcag caataagaaa gcaataaggg
     6301 cagatattat tatttacgga ggaacatctg ctgcagtaac agcagcagta caggccgcag
     6361 agatgggaca cagagcagtg attgtgtcac ccgacaggca tctgggaggg atgtcatctt
     6421 ccggtcttgg ttttactgat actggaaata aagaggtgat aggagggctt gcccgcaggt
     6481 tttatcagct tatctacgag cattatgccg attcatcagc ctggaaatgg cagaaacggt
     6541 cggaatatgg taacagggga cagggtaatc ctgcaattga aggggataag cgtaccatgt
     6601 ggatttttga acctcatgtg gccgaagctg cttttgagaa gatggtttct catcctctga
     6661 taaaggttta cagggatgaa tggctcgaca gagaacatgg agtcgaaaag aaagatggaa
     6721 aaattgtttc aataacaaca ctgagtggta agagatttaa ggggaaagtt tatatcgatg
     6781 tcacttatga aggagacctg atggctgctg caggcgtttc gtacactgta ggccgtgaag
     6841 ctaatagcgt gtatggagaa gtatggaatg gtgttcagaa gggagtcttt caccatggtc
     6901 attatttcaa aacgaatata agcccttact ggatccccgg agatccttcc agcggactgc
     6961 ttccgagaat atatccaggt gaccctggcg aaaacggctc gggcgacaaa aaagtccagg
     7021 catactgctt caggctatgc ctcacacaac atcccgaaaa caaagtacct atcactgccc
     7081 ctgaaggtta cgatagcaca cagtacgaac ttcttgtgcg ggtagctgaa tcgcagtgga
     7141 aagagttttt cgccaagtat gacccaattc ctaactggaa aaccgatgtg aataaccacg
     7201 gacccttcag ctatgataat atcggtatga actgggacta tcctgaagct tcatacgaaa
     7261 gaagaaagga aattatcgaa gagcacatac gttaccagaa ggggcttctg tattttaccg
     7321 caacatgcaa aagacttcct gaatgggtgc gcaccgaaat gaataaatgg ggatatgcaa
     7381 aggacgaatt caccgacaac ggcaactggc catataatat atatgtaaga gaggcacgac
     7441 gcatgatcgg tgaatatgta actacagaac acgatgtgct gagccggaga gaagtccccg
     7501 acccggttgg gatgggttca tacacaatgg attcccataa tgtgcaacgc tatgtgaccc
     7561 ctgaaggctt tgtccagaat gaaggcgata tcggagtgag agtgcccaaa ccttacaaaa
     7621 tagcaaaggg ttcactgatg ccaaagaggg aagagtgcct taacctgctg gtgcccgttt
     7681 gcgtctcttc atcgcatatt gcatacggct ccataagaat ggaaccggta tttatgatcc
     7741 tgggccagag cgcagcaaca ctcgcatcac ttgcaatcga aaaaagaaaa acaatttatg
     7801 aaatccctta tgaagaactg aaaacagaat tgataaaata cggccagata cttgaatacc
     7861 agtaaaagaa aacaaaaaat ataaaagtaa atttaacctg ttaaatcagt gtgttatgaa
     7921 aaagaaaatt ctgtttgcaa cactgtgttt gatctcatta tcacttcttc cccaggaaga
     7981 tccattccct cttattgacc tgcatgttca tatcaaggag gatttcacaa tcgaaaaagc
     8041 ccttcagaaa tcaagagctg aacatattca atacggaatt gcaataaatt gtggtgtggg
     8101 ctttcccgtc cagtccgaca gacaggttga ttcatttctt cttgagatga agaaataccc
     8161 tgagtttttt gtcggcatgc aggctgaagg cagggagtgg gtaaaacttt tttcaaaaga
     8221 ggcaatagct aagtttgatt atgttttcac cgacggcatg acattcttcg attccaaagg
     8281 aagacggaac agaatatggc tgaaggaaga aacatggata gaggatgagc aaaaattcat
     8341 ggattatctg gttcgaacta ttgttaaaat cctgaatgaa gagccaataa atatttatgt
     8401 caatccaact tttttgcctg aacagatggc cgacaggtat gattatttct ggactgataa
     8461 aaggatggat gctgtgatag aggcggcaaa gaaaaataat attgcgattg aaatcaacaa
     8521 ccggtacagg attccttccg agaagtttat ccgcagggca aaagctgcag gggtcaagtt
     8581 tacaatcggt acaaataatg ccaactccag attcggaaga gccgaatatg ccctggaaat
     8641 gataaggaaa tgcggcctta cggagtcgga cttctggaaa cctgtaaaga aaaaccgaac
     8701 agtagtcaca attattgatg ataaatttta tatcaatgga caggtgactt atccgggcag
     8761 gtactggaaa gggaacagga ttgaaggatt gctgatgaat tcaaggatgg ttcagggtat
     8821 ttttgacgac ctgaaccctg aaacagcccg aagatgggta taccccgata cccgtgtgtg
     8881 ggacccggat agaaacacca gggagtttgt cttaaacatg aaaaaatggt atgaccatgg
     8941 cctccttgct tttacaatta acctgcaggg aggaagccca acaggatact cacaggaaca
     9001 accatggcat aactcggcat ataatgccga cggcagc
//
LOCUS       DAOSNB010000070         9023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7681_length_9023_cov_4.297948, whole genome shotgun sequence.
ACCESSION   DAOSNB010000070 DAOSNB010000000
VERSION     DAOSNB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7681_length_9023_cov_4.297948"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2814)
                     /gene="ccsA"
                     /locus_tag="PLG42_04380"
     CDS             complement(<1..2814)
                     /gene="ccsA"
                     /locus_tag="PLG42_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU30483.1"
                     /translation="MMKILRFLFSPVLMALLFFFFALAMAVATFIENDYGREMAYGVV
                     YGTKWFELIMILLAVNLAGQVFQYRLWRKEKITIMGFHLAFIVMLAGAGLTRYTGYEG
                     TIHIREGEEENRVFSAEKYLGYALKDEGGNIIAERYEKYSPALERGSFERKINENLVL
                     RLAQVIPDAIESVEESPSGSPVVSFLVSLPNRGMQNLIIKEGESTIAGALSVSFNSDT
                     EADVRITADSTGFWFTPALETSVTNMMGGDKITFRPGEKIPLKQMQVISAGDVKFVPQ
                     RFTLKGIISVEPAGMERIEEGKSAMIFHVFEGSNTYTVTLWNNPGEYVSEASTSIGGK
                     TLSISFGSKIIKLPFSLKLNDFILERYPGSNTPSGYKSDVLLNDHEAGISKPYLIYMN
                     RILKYRGYRFYQASYDSDERGTILSVNHDIVGMMVTYTGYGILFLFIILSLLNKNSLF
                     RKANISYWRSPFSRKILAIAVFVVLCGFSSLEAQQRFIPDRRAADEFGKVLIQDQKGR
                     TEPLYTLSNDILRKVAHKTSFEGLTSMQVLLGIYFDFQNWQNVPLIRISNRNLAKKIG
                     LNGRMAAFSDIVDFKRDGNYKLSDDVERAYSKRPAERNKYDKEVIKTDERVNIVYMVY
                     TGEFMKLFPLRDSLGSWGNAETAMKNAFSREDSLYVGSILSAFYQAASVGNISQAKLV
                     TRSLLEYQRRFAGYKLPSDSKIKAELLFIKASVFEKLFPFYATTGIVALVILIIQLIR
                     GQGDSRLINLLVFWLLFVGFIAHTTGIALRWYVSGHAPLSNGYESMIFISWVTLLAGF
                     IFSRKSSFTLPATAVLSAMTLLVAHMSFMDPEITNLVPVLKSYWLTLHVSVITSSYAF
                     LGLGAVLGLIVMILLIFSNEKNISTTGETIESLTVINYRTLIIGLYLLTIGTFLGAIW
                     ANDSWGRYWGWDPKETWSLITIIV"
     gene            complement(2976..3377)
                     /locus_tag="PLG42_04385"
     CDS             complement(2976..3377)
                     /locus_tag="PLG42_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOU30484.1"
                     /translation="MPLLRKLFFAFLILLMPLFVFGQKKPEPWPVPEEYKNMVNPVPK
                     DEASIKEGQALYTKYCASCHGKTGLGDGVKSRALQTYSGDFSGPEYQNQTDGEHFYKT
                     KFGRGEMPKYDGRLQDEDIWKIIHYMRTFKK"
     gene            3474..4151
                     /locus_tag="PLG42_04390"
                     /pseudo
     CDS             3474..4151
                     /locus_tag="PLG42_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017530.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
     gene            complement(4163..4966)
                     /locus_tag="PLG42_04395"
     CDS             complement(4163..4966)
                     /locus_tag="PLG42_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOU30485.1"
                     /translation="MKKAGVIFSCLIIILLVFQACSTVPLTGRRQVNLLPESEMIAMG
                     LTNYSEFLKENPPSPDASSSAVVKEVGNNIASAVQKYFTDNRIESRLEGYAWEFNLVE
                     SQEANAWCMPGGKVVVYKGILPYTVDKNGLAVVISHEIAHAVARHGNERMSQQLLAQL
                     GGIALSEALKTKPEETRSIFLTAYGLGSQVGVLLPYSRRHELEADRLGLIFMAMAGYD
                     PQSAVSFWERMASMGGTKPPEFLSTHPSDETRIAKIKEAIPEAMKYYKK"
     gene            complement(5010..6788)
                     /locus_tag="PLG42_04400"
     CDS             complement(5010..6788)
                     /locus_tag="PLG42_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOU30486.1"
                     /translation="MEKDKLIRSGEFLIKETSASDIFIPEEFNEEQKMIAQTCRDFLA
                     AEVYPAIPQLEKGDRELMKSILKKSGELGLMGISVPEEYGGFGQSFVTQMFAAETTGA
                     GYSFSVAFMAHCGIGTMPIVYYGNEDQRQRYVPKLATGEFLGAYCLTEPGAGSDANSG
                     KTNARPSEDGKYYILNGQKMWITNAGFADTLIVFAKVENDRVLSAFIVESSWPGVVIG
                     ADEHKMGIKGSSTAQIFFNDVKVPAENMLGKRGEGFRIALSILHMGRIKLGANVLGAA
                     KKSITDSVKYANERKQFGVLISSFGAIKYKLAEQVIRCYAAESAVYRVSKDIDDMMEI
                     LKAEMPDKGRAAIEAISHYAVEAALLKVAGSEMLDYVVDEAVQIHGGMGYSAEMDVER
                     SYRDSRINRIFEGTNEINRLLVSDTAMKRAMKGEFDLFGPAEKIYNNPDESVEKLQDN
                     DGYFNRKKKMIRNMKKAALLCIHGATKKFEKGLVNEQEVLNNITDIMIDTYLAESLAL
                     RVEKREGMKGTDPVYRDIIDVFVYDAAFRVRKNASDAICSFASPDEAGRLLEAVEKFT
                     RTDCLNVKDARRRIADKLIADNDYKF"
     gene            complement(6814..7608)
                     /locus_tag="PLG42_04405"
     CDS             complement(6814..7608)
                     /locus_tag="PLG42_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU30487.1"
                     /translation="METRKIEIPVMSELFARGEKPEYLLWVGCAGAYDDRYKKVVRAF
                     VKILEYLNISYAVLGKEETCNGDPARRAGNEMLFQMQALQVIDILNRYEVKKILTICP
                     HCYNVFKNEYPDLGGHYEVVNYIQFLNKCIEERKLKPGKEAFKDSRITYHDPCYLGRA
                     NEVYEEPREVLRSVNADFREMKRNRSFALCCGAGGGQMFKEAEKGTKEIFIERTEEAL
                     ETGASVIATACPFCMTMITDGLKYKGREQEIKNLDIAEIIAQSLGL"
     gene            complement(7642..8961)
                     /locus_tag="PLG42_04410"
     CDS             complement(7642..8961)
                     /locus_tag="PLG42_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU30488.1"
                     /translation="MTGQIIFILALLVTFGVFYYTVSRIAALFRLTKPAFPVKDFGKR
                     FSMMLKVAFGQSKILRRPVVGFLHALVFWGFCIILLGSIEMVIDGVSGTEKSLRFLGP
                     VHDFIMAAGDIFALLVLVSVVVFLTRRLFLKIKRFEGIEMKKKSHIDAIVSLSLILFL
                     MITLLGMNAGYIKHAELTGEKIHGVYPVSAILAGLLSGSNAEHFWLLHRISWWSHILT
                     IFFFANYLPYSKHFHVFMSIPNVFLSRLEPLGKLANMESVTREVKLMLYPEVATSSSD
                     QEAAPGRFGVKDVEDVTWKNYLDSLSCTECGRCTSVCPANLTGKKLSPRKIMMDLRAR
                     MKEKGPEMVRKGRDYNDWKSLLRDYISEEELWACTTCNACARECPVNINHPSLIIDMR
                     RYLVMEEGSAPGQLKAMFSNIENNGAPWQYSPEDRLNWAKDLEININ"
BASE COUNT         2541 a         2096 c         1733 g         2653 t
ORIGIN      
        1 aacaataatt gttataagcg accaggtctc cttgggatcc cagccccagt aacggcccca
       61 ggagtcgttt gcccatatag ctccgagaaa agtcccgatg gtaagaaggt aaagaccgat
      121 tatcaatgta cggtaattaa taactgtcag cgattctatt gtttctcctg tcgttgagat
      181 attcttttca ttcgaaaaga taagcaatat cattactatc agccccagaa ccgcacccag
      241 accaagaaat gcataactgc ttgttataac tgagacgtga agggtgagcc agtacgactt
      301 aagaacaggt acaaggttag taatctcggg gtccatgaaa ctcatgtggg ctacaagcag
      361 tgtcatggcc gagagaactg cagtggcagg aagggtaaat gaagatttcc tgctgaagat
      421 gaaacctgca agtaatgtga cccaggaaat gaatatcatc gattcatagc cgttgctaag
      481 aggggcatgt cctgaaacat accatctgag agcgatgcca gttgtatgtg caatgaaacc
      541 gacaaagagt aaccagaata ccagcaggtt aattaacctg ctgtctccct gtccccttat
      601 caattgaatt atgaggataa caagcgccac gattcctgtg gttgcataga agggaaagag
      661 tttttcaaaa actgatgcct taataaataa tagttcagcc tttatttttg aatcagacgg
      721 cagtttataa cctgcgaacc ggcgctggta ctcaagcaat gaacgggtaa caagcttcgc
      781 ctgtgaaatg tttcccacgc ttgccgcctg ataaaatgcc gaaaggatac ttccaacata
      841 aagagaatct tcacggctga aggcattctt cattgccgtc tcggcattgc cccatgagcc
      901 cagactatcc ctcaggggaa acagcttcat aaattcccct gtatagacca tataaacaat
      961 gtttacccgt tcatcggttt tgataacttc cttgtcgtat ttatttctct cggccggcct
     1021 ctttgaataa gccctttcta catcatcaga gagtttatag tttccatccc ttttgaaatc
     1081 cacaatgtcg ctgaaagcag ccatcctgcc atttaaaccg attttctttg ccagattcct
     1141 gttagaaatc cttatcaagg gcacattctg ccagttctga aagtcgaaat aaatccccag
     1201 caaaacctgc attgatgtca gtccttcaaa acttgtttta tgtgccacct ttctcagaat
     1261 atcattactg agagtataaa gcggttcggt acgtcctttc tggtcctgta taagaacctt
     1321 accgaactca tcggcagccc tcctgtcggg aataaacctc tgctgtgcct caagtgatga
     1381 aaagccacaa agaacaacaa aaacagcaat ggcaagtatc ttcctgctga atggggaacg
     1441 ccagtagctg atgtttgcct tgcggaatag tgagttctta ttcagaagcg ataatataat
     1501 aaacaggaaa agtataccat aacctgtata agtaaccatc attccgacaa tatcatgatt
     1561 gaccgacagt atagtacctc tttcgtcaga atcatacgaa gcctggtaaa acctgtatcc
     1621 cctgtatttc agtattctgt tcatgtaaat gagatacggt ttcgatatcc cagcctcatg
     1681 gtcattaagt aaaacatcac ttttataacc cgatggagtg ttcgagccag gatatctttc
     1741 aagaataaaa tcattaagct taagagagaa cggcaatttg ataattttcg agccaaagga
     1801 aatgcttaat gtttttccac ctatgctggt tgaggcttct gagacatatt cgcctggatt
     1861 gttccagaga gtaactgtgt aagtattgga tccttcaaat acatgaaaga tcatcgcact
     1921 ctttccttct tcaattcttt ccatgccagc cggttcaacc gatattatcc ctttgagggt
     1981 gaatctctgg ggaacaaact tcacatctcc tgctgagatc acctgcatct gtttcaaagg
     2041 gatcttttca ccaggcctga atgtgatctt atccccgccc atcatgtttg tgactgatgt
     2101 ttcaagggca ggggtaaacc agaagccagt gctgtcggct gttattctta catccgcttc
     2161 tgtatcgctg ttaaagctca ccgaaagagc tccagcaata gttgattccc cttccttgat
     2221 tatgaggttc tgcatgcccc ggttgggcag gctcaccaga aacgaaacaa ctggtgatcc
     2281 cgaaggagac tcttcaaccg attcaattgc gtcaggaata acctgagcaa ggcgaagtac
     2341 aaggttttca tttattttcc tttcgaagct gccacgctcc agcgccggag aatatttttc
     2401 gtatctctca gctataatgt taccaccttc atccttaaga gcatacccaa ggtacttttc
     2461 tgcagaaaag actctgtttt cttcttctcc ttcccttatg tgtattgtgc cctcataacc
     2521 agtgtatcgc gtaaggccag cccctgcaag cattactata aaagccagat gaaaccccat
     2581 aatggttatt ttctccttcc tccacaaacg gtactgaaac acctgtcctg ccagatttac
     2641 ggcaagtaga atcataatta actcaaacca tttagtgccg taaacaacac cataagccat
     2701 ctcccttccg taatcgtttt cgataaatgt tgccaccgcc attgccaggg caaagaaaaa
     2761 gaacaggagt gccatcagaa caggggaaaa tagaaacctt aaaattttca tcatttttaa
     2821 ttcaataaca tattaagtaa tagataactt ataagtctgc agaatggttc aagtttacaa
     2881 caaaatactg attaacaata cgataaaatt gctgataatg agcaagttta atatagcatt
     2941 aatggttgaa ctattacaga aaatataaaa agcagttatt tcttaaacgt tctcatataa
     3001 tgaataatct tccagatgtc ttcatcctgc agcctgccat cgtatttggg catctcaccc
     3061 cttccgaatt tggttttgta aaaatgctcg ccgtcagtct gattctggta ttcaggtccg
     3121 gaaaaatctc cagaatatgt ctggagtgcc ctcgacttta ctccatcacc caatccggtt
     3181 ttaccgtggc atgacgcaca atattttgtg taaagggcct ggccctcctt tatgcttgct
     3241 tcgtctttcg gaacagggtt gaccatattt ttatattctt ccggaaccgg ccatggttca
     3301 ggcttcttct gtccaaaaac aaaaagaggc ataagtagaa tcagaaacgc aaaaaacagt
     3361 tttcttaata aaggcatacg attaagtttt aaaattttgg gatgataaaa ttaataaaac
     3421 tcctgaacag gaaaaatgtt tatctttatt aatcaattat caaaaattca ggtatgatat
     3481 cagattccgt tctgaaacaa ttcttattcc ttgcacttca tccggaaaaa ggaagggtaa
     3541 ggatagattc catgaggttc agatatgcat tagcaggagc atatcttatg gagcttttaa
     3601 ccaggaagga aattaaaata gaagataaaa ggataaatca ttcgttgaga aaaaacggag
     3661 agccagttca tgatactatt gttgaaatat ttgaaaagca gaagcgtccg agaagggtca
     3721 ggtactggat aaaccggctc tcgtgaaaaa gcagatttat cttcagagaa acaatgaaat
     3781 atctctcttc atcggggttg ttaaggcatg agaaacacta ttttcttggc atcatacctg
     3841 ttaacaggta ctttatttca gacataagga accagaaaga tgttgttgat aaaatcaggc
     3901 gtgttctact ttataatgat tctccttcga gggaagtgct tatgctggta agtctggtca
     3961 ggatgacgga atcggaaaag ctgattgctg agtctaaaga agaaaggaga cttcttcgta
     4021 acaaaataaa acaattaacc gacgacaaga gcggtctgat tgatccggaa gttgctgaaa
     4081 ttcagaaatc tgtattgtca gcaattactg ctgcacgttc agcagccgtt actgtagcgg
     4141 cttcaggctg ataaaactgg tactactttt tataatattt cattgcctca gggatagcct
     4201 ctttgatttt tgctatcctt gtttcatcgg atggatgcgt actgagaaat tcaggtggtt
     4261 ttgttcctcc cattgaagcc attctttccc agaacgaaac agctgattgg ggatcatagc
     4321 ctgccatagc cataaaaata agtcccagtc ggtcggcttc aagctcgtgc ctgcgagaat
     4381 aaggcaacaa cactcccacc tgtgaaccaa ggccgtatgc tgtaagaaaa atgctgcgtg
     4441 tttcctcggg ttttgttttc agggcctcgc tgagggctat acctccaagc tgtgccagaa
     4501 gctgctggct catccgctca ttgccatgac gtgctactgc atgggcaatc tcatgactta
     4561 taaccactgc cagaccgttt ttatctacag tatatggcag aatgcccttg tataccacaa
     4621 ctttacctcc gggcatgcac catgcattgg cttcctggct ctctaccagg ttaaattccc
     4681 aggcataacc ctcaagacgg ctttcaatcc tgttatccgt aaaatacttc tgcactgccg
     4741 atgcaatatt gttaccaact tcctttacca ctgctgaact ggaagcatct ggagaagggg
     4801 gattttcctt aaggaattcg gaataatttg tgagtcccat agcaatcatc tccgactcag
     4861 gcagcagatt aacctgcctt cttcctgtaa gcggtacagt ggaacatgcc tgaaatacaa
     4921 gcaatataat tattaaacaa gaaaaaataa ctcctgcttt tttcatggtt tttacttctg
     4981 ctgtaaagat aaccaattga ttttaaaaat cagaatttat aatcgttatc agcaataagt
     5041 ttatcggcta ttcttcgtct tgcatccttt acattcagac agtctgttct ggtaaatttc
     5101 tccaccgcct caagcagtct gccggcctca tcaggtgaag caaatgaaca tattgcatcc
     5161 gaagcatttt ttcttaccct gaatgcagca tcgtacacaa atacatctat gatatccctg
     5221 tatacagggt ctgttccttt cataccttca cgtttttcaa ccctcagtgc cagtgattct
     5281 gccagatagg tatcaatcat tatgtcagta atattgttca gaacttcctg ttcgttcaca
     5341 agaccttttt cgaacttttt agttgcaccg tgtatacaaa gcagtgcagc cttcttcata
     5401 tttctgatca ttttcttttt gcggttgaaa taaccatcat tatcctgcag cttttcaact
     5461 gactcatccg gattgttata aattttttca gctggtccga agaggtcaaa ttctcctttc
     5521 attgctctct tcatggcggt atcagaaacg agaaggcggt ttatttcgtt tgtaccttca
     5581 aatattctgt tgattcttga atcgcggtaa cttctttcga catccatttc agctgaataa
     5641 cccataccac cgtgtatctg aacagcctca tccacaacat aatcgagcat ttcagagccg
     5701 gcaaccttca ggagagccgc ttcgacggcg taatgactga tagcctcaat cgctgccctg
     5761 cctttatcag gcatttcagc cttcaggatt tccatcatat cgtctatatc tttacttacc
     5821 ctgtagactg ccgattcggc tgcatagcac cttattacct gttcggcaag tttatatttt
     5881 attgccccga agctggaaat tagaacacca aactgctttc tctcgttggc atattttaca
     5941 gaatcagtga tagatttttt tgcagcgccc agaacattgg ctccaagctt tattcttccc
     6001 atgtgaagta ttgagagagc tatcctgaat ccttctcccc tctttccaag catattttca
     6061 gccggcactt tcacatcgtt aaaaaatatc tgtgcggtgg aagagccttt tatacccatt
     6121 ttgtgttcat cggcaccgat gacaacacct ggccatgacg attcaacaat aaaggcactg
     6181 agaactctgt cgttttcaac ttttgcgaag acaataagtg tgtcggcgaa ccctgcattg
     6241 gtaatccaca tcttttgccc gttcaatata tagtatttcc cgtcctcgct ggggcgggcg
     6301 tttgtttttc cggagttagc atcgctgcct gctccaggct cagtgaggca gtatgccccc
     6361 agaaattcgc ctgtagccag cttcggcaca tatctttgcc gctggtcttc attaccgtaa
     6421 taaactatcg gcatggtacc tattccgcaa tgcgccataa atgccaccga gaatgagtaa
     6481 ccggccccgg tggtctcggc tgcaaacatc tgtgtaacaa atgactgacc gaaaccgcca
     6541 tattcctcag gaacagagat gcccataagc ccgagttcgc ccgatttctt cagaatactc
     6601 ttcattagtt ccctgtcgcc cttctcaagc tgaggtatgg cagggtaaac ttcagcagca
     6661 agaaaatcac ggcatgtctg tgctatcatc ttttgttcct cattgaactc ttcgggaatg
     6721 aagatatcac tggctgaagt ttcctttatc agaaactcgc cactacgtat aagcttgtct
     6781 ttttccatct ttcaggttgt ttgatttttt tgattatagt ccgagagatt gtgctataat
     6841 ctctgcaata tcaagatttt ttatttcctg ttcacggcct ttatatttca gcccgtcggt
     6901 tatcatcgtc atgcaaaaag gacaggcagt ggcaattaca ctggcacctg tttcgagagc
     6961 ctcttccgta cgttcaataa agatctcctt tgtgcctttc tctgcttcct tgaacatctg
     7021 ccctcccccg gcaccacaac acagggcaaa gctccggttt cttttcattt ccctgaaatc
     7081 agcattaaca gacctcagaa cctccctcgg ttcttcatat acctcatttg ccctcccaag
     7141 ataacagggg tcatgatatg taattctcga atctttaaag gcttctttac caggtttaag
     7201 cttcctttcc tcaatacatt tattaaggaa ctgtatataa ttcaccactt cataatggcc
     7261 tcccaggtcg gggtattcat ttttgaaaac attatagcaa tgtgggcata ttgtaaggat
     7321 cttcttaacc tcataacggt tcagaatatc tattacctgc agagcctgca tctgaaaaag
     7381 catctcattc ccagccctgc gggccggatc gccattgcag gtctcttcct ttcccagcac
     7441 agcataactg atattgagat attcaagaat ctttacaaat gctcttacaa cctttttata
     7501 cctgtcgtcg tacgcacccg cacaacctac ccatagcaga tactcaggct tttcacccct
     7561 ggcaaaaagt tcagacatga caggtatttc aatcttccgg gtttccataa gaaaatatta
     7621 ttatactgtt tctggagcta atcagtttat atttatttca aggtcttttg cccagttaag
     7681 gcgatcttcg ggcgaatatt gccatggcgc gccattattt tcaatattgc tgaacattgc
     7741 cttcaactga ccgggcgctg agccttcctc catcaccagg tacctccgca tatcaataat
     7801 taatgagggg tgattgatgt ttaccggaca ttccctagca cacgcattgc atgttgtgca
     7861 tgcccataac tcttcttccg agatataatc acgtaacagt gatttccagt cgttgtagtc
     7921 ccttcccttt ctcaccattt caggaccttt ctccttcatc cttgccctga gatccatcat
     7981 tattttcctc ggggaaagtt tctttcctgt aagatttgca ggacaaacgg aagtgcacct
     8041 tccgcattca gtgcacgaaa gagaatcaag gtaatttttc catgtgacat cttcaacatc
     8101 ttttactccg aagcgtcctg gtgctgcttc ctggtcagat gaagatgttg ccacttcagg
     8161 ataaagcatc agcttcacct cccttgtcac gctttccata ttcgcaagtt tccctaatgg
     8221 ttcaagtctc gataaaaaca cattgggaat tgacatgaaa acatgaaaat gcttggaata
     8281 aggcagataa tttgcaaaga agaaaatagt aagaatatgc gaccaccagg aaatgcggtg
     8341 aagcagccag aaatgttcag catttgaccc agacagtaat cctgctaaaa tagcgcttac
     8401 cggatagact ccgtgaattt tttctccggt cagctcagca tgctttatat atccggcatt
     8461 cattccgagc agggttatca tcaggaataa gataagtgac agcgagacta ttgcatcgat
     8521 gtgggatttc ttcttcattt caattccttc aaagcgtttg attttcagga aaagacgccg
     8581 tgtcaggaat accactaccg aaacgagaac cagtaatgcg aatatatcac cggcagccat
     8641 aatgaaatcg tgaacaggac ccagaaacct gagagatttt tctgttcctg aaacaccgtc
     8701 aattaccatt tcgatgcttc caaggagaat aatacagaaa ccccagaaca ccagggcatg
     8761 aagaaatcca accactggcc ggcgcaagat ttttgactgt ccgaatgcta ctttcagcat
     8821 catgctgaat ctcttcccga aatcctttac cggaaaggcc ggttttgtaa gacggaaaag
     8881 agctgcaata cggcttactg tatagtagaa aactccgaat gtaacaagaa gggctagtat
     8941 aaaaataatc tgtcctgtca tttaatggtt atttcataaa atttatcctt gattattcct
     9001 gattaatttt atgaatttac gga
//
LOCUS       DAOSNB010000071         8908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7819_length_8908_cov_4.337513, whole genome shotgun sequence.
ACCESSION   DAOSNB010000071 DAOSNB010000000
VERSION     DAOSNB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7819_length_8908_cov_4.337513"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5063)
                     /locus_tag="PLG42_04415"
     CDS             complement(<1..5063)
                     /locus_tag="PLG42_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOU30489.1"
                     /translation="MLAGLILISQPAASQCIFYISTTDIELPGNGGYGDIYLSTGGCE
                     SYEAYTNDSWIYVWTDYYEHIVTVYADANHEGYRQGTVVIHDYWTETDYYVTVSQDVG
                     CEQLPAPDEIYGPSSICCSTGATYYINPVEGATSYSWYVDGNPVPGYYGTSAMINSGC
                     GSAQVIISVCGYNECGSGLLSTKEINIKFSPSGGSISSSQTICYNTVPELIINIASAS
                     GGVPPYSYQWQSSTDGGFTWNNIPGATSTTYSPPALTIPTKFRRTVTDANGCMSGISN
                     IVTIDFYSTSVSGGIISGDNTSYGGYSGPITGLQDASGGSGIYSYEWEKSENGQSQWT
                     NTGCTNKDFPSTPQSVNMYYRRKVTDNLCGYTAYSNILFKKVISFASYLYGTEIPEPE
                     TRIINTNLHVGSTNGSLNVDAAGGATYNINIELPPGVNGLSPALSLVYSSHSGPGIAG
                     YGWNISGLSSIKRGPQNFYNDGSARGVELDNNDRFYLDGQRLINTSSYYGDPSAQYQT
                     ELDIFTRVTPHNAGENGPEWFKAETSTGFIYEYGNSEESKQKINGFNPIMEWYISKIS
                     DLFGNHIDFSYLRENNLVYPSEIVYGPNKITFYYKSRFDTISSYFKGVKIKQNLLLEK
                     ITIQYNSNIIKTYEFKHSYQGSDYNSYSLLNEVIEYGTGTNRLNSTVFSYTLPENVSF
                     QQTEYNQDHQYITYKSKLVAGDFNGDGKSDFFCIPIPGRATWTGVKVCYGNGNDKFNY
                     IWSNSSIVIDLNKLKDIEAIDLNADGMDDILYEIYEESPPTTKFLVMLSNGFSLSDPD
                     EFFVSFSSDLYKTSGWKERAIHLRGKDIEFSGKDFNGDGINDVFIYNEYGDWTISSFG
                     GDNGVLYPPGYRNVKTLGSGNISTLSNHIITNDFNGDGKADIWSFETTGLNIYSLSEG
                     SLSVIYNSDWPTKNHHFIPGDFNGDGKADMFLYGYNTYDWSEWQIRLSTGTGFKTIFI
                     PQKKANLKDDHVRVGDFNGDGCTDIMASGSTYDNAYFFISKNKGANLYTSILVRYPTG
                     AHNCYVADFNGDGRTDFICTDLEGAPWWDGYVVYRTSGNSSILMDKISDGLGVLTKIN
                     YARLSEYGTDIYQKGSGAVFPVTDFQGPWSVVSSIFTDNGKGSFNTQNYYYEGAKIHL
                     QGKGFLGFAKIKAIDSATGIENGNITGYNSTYFYPESISSYSKLSGSDTISVVRNTWS
                     RIILDASRKRIFPYIQTSVNTDKLTGHTVTSNSSYDNFGNLTQSVKTYSNGITETTIS
                     SYENIVSSSQWLLGRPYTTTLQYTGGDTTISRTCSRVFSTVNNNLLSETWFEGTNYQI
                     TKSYGYYANGTLQYETTTANNVSRTTSYTYESDNIRVHTITDPLSHSTTSTYDSYGRL
                     YTRQDYLGNTVTYQYDGMGRPYIVSSTDGNQTTTTLSWENPASTPLNARYSVTELGND
                     GSQSKKWFDRFGREIRSDVKGFDGRLIITETRYNYKGQADSISEPYYSGDQVLWNRYQ
                     YDSYGRKTWLYRPSGRNTQWQYNGATVTETTAGKTFSKTLAPDGTLVSATDAGGTITY
                     TYYPDGKPRTIIAPGNIITRMYYDITGNQIKLVDPSAGTITYGYNGFGELTSQVNARN
                     QATYIYYNQDGTISQKITPEGTITYSYNSNKQLTGISSTNNVSRSFVYDSKGRLASIT
                     ETIPGSSAFTTSYTYDGLG"
     gene            complement(5175..5582)
                     /locus_tag="PLG42_04420"
     CDS             complement(5175..5582)
                     /locus_tag="PLG42_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30490.1"
                     /translation="MKKPSFLYILFVSGFLWLAGTNTTNGQEAKILPKQRTTPERAAE
                     GQTKWMINSLHLDQKLFEDIYRINLKYQMISDSVHVTDAFITAKQEAYVQCTQNRNEE
                     LKKVMPPDAFDKLMSLMEAEKEKAAAFRASVQK"
     gene            complement(5616..6074)
                     /locus_tag="PLG42_04425"
     CDS             complement(5616..6074)
                     /locus_tag="PLG42_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30491.1"
                     /translation="MNKAKTGLSGLVIILLSLTCINAQTIEFTYDPGGNRTSRTLIVE
                     KMMHGESKSGLMSESGSEIKATDLDNREMTVIIYPNPSRGLLNIEIINMPSEAKNELS
                     IYDLNGTRLLIKKDFVTPSEVDISRFRDGVYIMRIKINKSVSDWKIIKSN"
     gene            6885..8102
                     /locus_tag="PLG42_04430"
     CDS             6885..8102
                     /locus_tag="PLG42_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOU30492.1"
                     /translation="MNENENELFKIPEEALKKIRTQEEFEDYFQTLYKKGVEALLKAE
                     IKEHLGYSKHEPAGRNTGNSRNGYSKKILKTNLGDIPLEVPRDRNSTFEPVVVPKHQR
                     MSSKIEHAIITMYSRGMTTRDIEETIKDIYGVEVSESSISNITSAIIEDIKEWQQRPL
                     DPVYVVVWMDGIVIKVRQNGKVTGKTIYLMIGLKHNGLKEVLGMWISESESASFWLNV
                     LTEIKARGVKDILIACTDNLSGIRQAIKSVFPNTVTQLCVVHQIRNSIRYVVWKDRKH
                     FLSDLKNVYGAINRDMAYDALENFAEKWGSKYAYAIKSWKDNWDELTAYFDYPMEIRR
                     IIYTTNAIESLNSSIRKYTKTKTVFPDDQAALKAVYLAIANVEKKWTYPIRDWGTVIN
                     QLIIIFGDRCSIF"
     gene            complement(8164..8466)
                     /locus_tag="PLG42_04435"
     CDS             complement(8164..8466)
                     /locus_tag="PLG42_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30493.1"
                     /translation="MYGKEFPGETFRVLKDKEMCKYGEYRTRRLVLEAYDRLAPAFDM
                     EAHLKRIKEVWEKYQENLSEKKGYSIKEEDMPIEKLEENGEDYGQGRLFGVKEESD"
BASE COUNT         2635 a         1757 c         1643 g         2873 t
ORIGIN      
        1 ctgccaagtc cgtcgtaagt gtatgaggtg gtgaaggccg aggaacccgg gattgtctcg
       61 gtaattgaag caagcctgcc tttgctgtca tacacaaatg aacggcttac attattggta
      121 gaactgatgc ctgtaagctg tttgtttgag ttataggaat aagtaatggt accctcagga
      181 gtaattttct gtgatattgt accatcctga ttgtaataaa tatatgttgc ctgatttcgg
      241 gcatttacct gcgaggttaa ttcaccgaac ccattatagc cgtaagttat cgttccggca
      301 ctcgggtcaa caagtttaat ctggtttccg gtaatatcat aatacatcct ggtaatgata
      361 ttgccgggtg ctattattgt tcttggttta ccatcgggat aatacgtata ggtgattgtg
      421 cctccggcat ctgttgccga gaccagtgtg ccgtcaggtg ccagagtttt ggaaaatgtt
      481 tttcctgctg ttgtttcagt aactgtggca ccgttgtatt gccattgggt atttcttccc
      541 gaaggtctgt acaaccaggt ctttcttcca taactgtcat actggtacct attccaaaga
      601 acctggtctc ctgaataata aggctctgaa atgctatcag cctggccttt atagttgtat
      661 cgcgtttcgg taataatcaa ccttccatcg aatcctttta catctgatct tatttcccgc
      721 ccgaacctgt cgaaccattt tttactctgg gagccgtcat ttcctaactc ggtaacggaa
      781 tatcttgcat ttaatggtgt ggaagccggg ttttcccatg acagtgttgt ggtggtctgg
      841 ttgccgtcgg ttgagcttac tatataaggt cttcccatac catcgtattg gtaagtaaca
      901 gtattaccaa gataatcctg tcgggtatat aatcttccgt aactatcgta agtgctggtt
      961 gttgaatgag aaagcgggtc ggtaatggta tgaaccctga tattatcaga ttcataagtg
     1021 taactggttg ttcttgagac attgttggct gtagtggttt catattgcag agtaccgttt
     1081 gcataatacc cgtacgattt tgttatctga taatttgttc cctcaaacca tgtttcagac
     1141 aacagattat tattgactgt actgaaaact ctcgagcaag ttctgcttat ggtagtgtca
     1201 cctcctgtgt actggagggt tgttgtataa ggccttccaa gaagccactg tgaagatgat
     1261 acaatgtttt cgtaacttga gattgtcgtt tcagtaatac cgttgctata ggttttaact
     1321 gactgtgtaa ggttcccaaa attgtcatac gaggaatttg atgtaacagt atggcctgtc
     1381 agcttatcgg tattaaccga tgtctggata taagggaaga tacgttttct ggaagcatcc
     1441 agaataatcc tggaccatgt atttctcaca accgaaatag tatcggaacc actcagcttt
     1501 gaatatgaag agatcgattc aggataaaaa tatgtcgaat tatatcctgt tatatttccg
     1561 ttttcaatac cggtagctga atcaattgct ttgattttgg cgaaacccag aaatcctttc
     1621 ccctgcagat gtatttttgc accttcataa tagtagtttt gtgtattaaa agaacctttg
     1681 ccattatctg taaatattga gcttacaaca ctccaaggtc cctggaaatc tgttacagga
     1741 aacactgccc ctgatccttt ctgataaata tccgtgccgt attcagacag tcttgcataa
     1801 ttgatttttg taagtacccc aagcccatcc gatattttat ccatcagaat ggaagaattg
     1861 cctgatgttc tataaactac atacccgtcc caccacggag ccccttcaag atctgtacag
     1921 ataaaatccg ttctgccatc accattaaaa tcagcaacat aacaattgtg ggctcctgtc
     1981 gggtacctga caaggatgct ggtatacaga tttgcacctt tgtttttaga gataaaaaaa
     2041 taggcattgt catatgtgct gcctgatgcc attatatcag tacagccatc accgttgaag
     2101 tcaccaacac gaacatgatc atctttcaga tttgctttct tttgcggaat aaatatcgtc
     2161 ttaaaaccag ttcctgtgga tagtcttatt tgccattcgg accaatcgta agtattgtat
     2221 ccgtataaaa acatgtccgc ttttccatcc ccgttaaagt cacccggaat aaaatgatgg
     2281 tttttagtag gccagtcaga gttataaata acacttaatg aaccttcaga caatgaatat
     2341 atattaagcc cggttgtctc aaaactccag atatctgcct ttccgtcgcc gttgaagtca
     2401 ttggttatga tatgattact taatgttgaa atattaccag aacctaaagt cttaacattt
     2461 cgataaccag gaggatatag caccccatta tcacctccaa aagaagatat tgtccagtct
     2521 ccgtattcgt tgtatataaa tacatcatta ataccgtcac cgttaaaatc ttttccactg
     2581 aattctatat ctttgcctct taaatgaatt gctctctctt tccaccctga ggttttataa
     2641 agatcagatg aaaaactgac aaagaattca tctggatcag acaatgaaaa gccgttagaa
     2701 agcattacta aaaattttgt agttggaggt gactcctcgt atatttcata taaaatatca
     2761 tccatcccat cagcatttaa atcaattgcc tcaatatctt taagtttatt taaatcaatt
     2821 acaatactag aattactcca tatataatta aacttatcat taccgtttcc atagcatact
     2881 ttcacaccag tccacgttgc cctgcccggg attgggatgc agaaaaaatc gcttttaccg
     2941 tcgccgttaa aatcacctgc aaccaacttt gatttatatg taatatattg atgatcctga
     3001 ttatactctg tttgctgaaa cgacacattt tcaggaagag tataagaaaa tacagttgaa
     3061 ttcagtctgt tggtcccagt accgtattca ataacttcat taagtaatga gtaagaattg
     3121 taatctgatc cctggtatga atgtttaaat tcatatgttt ttataatatt tgaattgtac
     3181 tgtattgtga ttttttcaag caacaggttc tgttttattt taacaccttt aaaatatgaa
     3241 ctgatagtat caaatcggct cttataataa aatgttatct tgtttgggcc atatacaatt
     3301 tctgaaggat ataccagatt attttccctt aaataactga aatcaatatg gttaccaaaa
     3361 agatccgata tttttgaaat ataccattcc atgattggat tgaaaccatt tatcttttgc
     3421 ttcgattcct ccgaatttcc atattcataa ataaatcctg tgctggtttc agctttaaac
     3481 cattccgggc cgttttcgcc agcattgtga ggtgtaacac gggtaaaaat atccagttct
     3541 gtctgatatt gtgcagatgg atcaccgtaa tatgatgaag tgtttatcag tctctggccg
     3601 tcaagataga atctgtcgtt gttgtcaagt tcaacgcccc ttgcagagcc atcgttataa
     3661 aaattttgtg gtcctctctt aattgatgat aggcctgaaa tattccatcc ataaccagct
     3721 atcccaggtc cgctgtgact tgaatacaca agtgatagtg ctggagatag tccgttaact
     3781 cccgggggta attcaatatt aatattgtaa gttgcacctc ctgcggcatc aacattcaaa
     3841 cttccattgg ttgatcccac atgaagatta gtgttaataa tacgtgtttc cggttcaggt
     3901 atttcagtac cgtataaata ggatgcaaat gatatgacct ttttaaataa aatatttgaa
     3961 taggcagtat aaccgcataa attgtcagtt acctttcttc tgtaatacat atttacagat
     4021 tgtggagttg aaggaaaatc cttattagtg catccggtat tggtccattg actttgtccg
     4081 ttttcagact tttcccattc ataggaataa atacccgaac ctccgcttgc atcctgcagt
     4141 ccggtaatag ggcctgaata gccaccataa ctggtattgt cacctgaaat tataccgcca
     4201 cttacagaag ttgaataaaa atctatggtc acaatatttg atattcctga catacagcca
     4261 ttcgcatcag taactgttct tcgaaatttt gttggaattg ttaacgctgg tggagaataa
     4321 gtggtagatg tagcaccggg aatattattc catgtgaaac ctccgtccgt tgaactttgc
     4381 cactgatagc tataaggagg aactcctcct gaagcagaag caatgttaat tatcagttcg
     4441 ggtactgtat tataacaaat tgtttgggag ctagatattg atccacccga aggagaaaat
     4501 ttaatattta tttcctttgt acttagaaga ccacttccac attcattata gccacaaact
     4561 gaaataataa cctgcgctga cccacatcca gaattgatca ttgcagatgt accataatat
     4621 ccaggaaccg gatttccgtc tacataccaa gaataggaag ttgctccttc aaccggatta
     4681 atataatatg tagcaccagt actacaacat atagaagagg gaccataaat ttcatctggt
     4741 gcaggcaatt gctcacaacc aacatcctgg gatactgtaa cataataatc tgtttcagtc
     4801 cagtaatcgt gtatcactac agttccctgc cgatatcctt catggtttgc atctgcataa
     4861 acagttacaa tgtgctcata ataatctgtc catacatata tccaactatc attggtataa
     4921 gcttcataac tctcacaccc tccggttgat agataaatgt caccataacc tccgtttcct
     4981 ggaagttcta tatcagttgt gcttatataa aaaatgcact ggcttgcagc aggttggctt
     5041 atcagaatta atccggccaa catggcaatt aacaggaaag cattttttaa atgttctcta
     5101 gtgctcattc ttcataccgg ttcttaatgg ttatgaataa ctctttattg aacattccgc
     5161 caatatatca gacactattt ctgaactgat gctctgaaag cagcagcttt ttctttttca
     5221 gcttccatca aagacatgag tttgtcaaac gcatcgggag gcataacttt tttcaattcc
     5281 tcgttcctgt tttgggtaca ttgaacatat gcttcctgtt tggcagtaat aaaagcatcc
     5341 gtgacatgaa cagaatcact aatcatctgg tacttaaggt ttatcctgta aatatcctca
     5401 aataacttct ggtccaggtg caatgaattt atcatccatt ttgtttgacc ttcggctgct
     5461 ctttccggtg tggttctctg ttttgggagg attttggctt cctgcccatt tgtggtattt
     5521 gtgccggcta accataaaaa gcctgacaca aataaaatgt ataaaaaact tggctttttc
     5581 atggaatcct tatttaggtt aatacagtta ttaagttagt tgctctttat aattttccag
     5641 tccgaaacac tcttattgat ctttatcctc attatataaa ccccatctct gaaacggctg
     5701 atatcaactt ccgatggcgt aacgaaatcc ttttttataa gtaaccgcgt cccgttcaga
     5761 tcatatatgc tcagttcatt ctttgcctct gaaggcatgt taattatttc aatattaagg
     5821 aggcccctgg aggggttcgg gtaaataatt acagtcattt ccctgttatc caaatcagtt
     5881 gcttttattt cactgcctga ttcactcatt agtcctgatt ttgattctcc atgcatcatc
     5941 ttctcaacaa taagtgtacg actggttctg tttcctcccg gatcgtaagt aaactctatc
     6001 gtttgagcat taatgcaggt taacgataac agtataatta ccaatcctga aagacctgtt
     6061 tttgccttat tcataacatt catttttaat aagttacttc tatgtttttt aaaaataccc
     6121 ttacgggatt gcaggtgact tttttatttc gtttaaataa ggaaggattt gttacccgat
     6181 ttcagacaat aattatcaac agattattaa cagcttatta acatcgcatt tgggaagtgt
     6241 tctcaacatg ataaatttta tagggtctat ggaattcaaa cttatacaac acgtggccct
     6301 gggtcgttct tgctcagatt atcctttttg aaattcaatc cttattacat ggtcaatata
     6361 aagtctttta tattccactt ttgtagtgca tgtattaatc cccaaaatag ctggacataa
     6421 tttgaaaaac aaattaggtt ttattaaatt tttaagccta tgtaaatcat tacaataata
     6481 aacggcagca cggaattctg aaaaaactcc tatgcagatt aggaatgaat actactagtc
     6541 attgtcaacc gataacctac tgacgacaaa agtatcggag gggttgtcag aagttttggg
     6601 ttgtgaagac actttgacca atccgaagat atagctaact ttgtttatcg gttgatgaat
     6661 ggaaacgaaa taaacattaa acgagaagta ctaaactctt ttgaaaaatc tatccagttt
     6721 attgggggtt aagacaataa tttattgggt agtgtccata attttgtgta aatgaaaagg
     6781 cagctgcatt tgggacgcca accggactgg agccggaaaa cgagctcaaa gtccggttgg
     6841 ttaggaagaa aaaattaatt ttaacctttt aaaaatgcag cattatgaat gagaatgaaa
     6901 atgaattatt taagattccg gaagaggcct taaagaaaat aaggactcag gaggaatttg
     6961 aagattactt tcagacgctt tataaaaaag gcgtagaggc acttctgaaa gctgagataa
     7021 aggagcatct tggttactct aagcatgagc ctgcgggtcg taacactggt aacagtcgca
     7081 atggttacag taaaaaaata ctcaagacta atctgggaga tataccgctg gaggtacccc
     7141 gtgaccgcaa ctctacattt gagcctgttg tagtaccgaa acatcaaaga atgtcttcaa
     7201 aaattgagca tgccatcatt actatgtatt ccagaggcat gacaacaagg gacattgaag
     7261 aaactataaa agacatttac ggtgttgagg tgagtgagag ttcgatatcc aatattacca
     7321 gtgcgataat agaggacata aaagagtggc agcaaaggcc tctggatccg gtttatgtgg
     7381 ttgtctggat ggatggtatt gtaataaaag tcaggcaaaa cgggaaagta acaggtaaaa
     7441 ccatctatct gatgatcggg cttaagcata atggtctgaa agaagtgctt gggatgtgga
     7501 tcagtgagag cgagagcgct tcattctggc ttaatgtcct tactgaaatt aaagccagag
     7561 gcgtaaagga catattgatt gcatgcaccg acaacctctc aggtataaga caggctatca
     7621 aatccgtatt cccaaatacc gttactcaac tgtgtgtggt tcatcaaatc cgtaacagta
     7681 tcagatatgt tgtatggaaa gaccgtaaac actttctgtc agatttaaag aatgtttatg
     7741 gtgcaattaa cagggatatg gcttatgatg ccctggaaaa cttcgcagaa aaatggggct
     7801 caaaatatgc ttatgctatt aaatcctgga aagataactg ggatgagctc actgcttact
     7861 tcgattatcc aatggagata cgtagaatta tctataccac aaatgccatc gaaagtctta
     7921 acagcagtat ccgtaaatat actaaaacaa aaacagtctt cccggatgac caggctgcct
     7981 taaaagctgt ttatctggct attgctaacg tggagaaaaa atggacttac cctatcagag
     8041 attggggtac cgttattaac caattaataa ttatttttgg tgatagatgc agtatatttt
     8101 aatcccaaac gctgcccgtt tacacaaaaa tttaaacagg ctcatttatt gatttataaa
     8161 atatcaatct gattcttctt tcactccaaa caacctaccc tgcccataat cctctccatt
     8221 ctcctctaat ttttcaatcg gcatatcctc ttctttaata ctgtatccct tcttttcact
     8281 cagattctcc tggtactttt cccacacttc ttttatcctt ttcaggtgcg cctccatatc
     8341 aaaagcaggt gccaggcgat cgtaagcttc gagaacaagt cgccgggtac gatactcccc
     8401 gtatttgcac atctccttat cttttagtac acgaaatgtt tcgccgggaa attccttacc
     8461 atacacatcc tgtggatcaa gaatatacct gagttcatcc ctgctaaggc cttacagtaa
     8521 tgcataccaa gcatctagct ccgccttcag ccgtggacgg cggtcttcat cttctattga
     8581 ttgtccgatg accggaggaa acaaattaga ttttgggcat cattttcgta taaaagaaaa
     8641 tttgttggtt tagatatctg gtaattttca cctgataaaa tcttccgggt tatttaacaa
     8701 tctgaaagtg gataaatgta ttttttatca taagatcttt cagatcaata tatttaagag
     8761 aacttctaag aataaaattt taaagtttga taatgttttc aatttcattc ccgatagcta
     8821 tcgggattat tcggttatat tcctttaaaa attcagccgg tttgagggct ccctttttcg
     8881 ccccaagaca tttttgtgtt aaaaagga
//
LOCUS       DAOSNB010000072         8784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7959_length_8784_cov_5.394661, whole genome shotgun sequence.
ACCESSION   DAOSNB010000072 DAOSNB010000000
VERSION     DAOSNB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7959_length_8784_cov_5.394661"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLG42_04440"
     CDS             <1..1341
                     /locus_tag="PLG42_04440"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOU30494.1"
                     /translation="LNPALRPSNTFYLGLGITCIGIGINNNYFNFSDVIMQGRSDSLI
                     TFLHPDYDVDKFLGKVKAGNFLTPQADVQLFGLGFNTGKDTYIFLDLIERVKGNITLP
                     GDLIRIMLKGNEEFVGKTMDLGALDASLMYFREAGIGFSAPAGKRARFGIRAKALFGI
                     AGISLDNKFLSITVNDDYTHIFNADLTANISGPVTVYFGADNKPDSLSVDEDKLKSKD
                     FYLNTRNAGLGLDIGAVVNITDRLSVSAAVTDLGFLRWNDMITNIKAESQFTFSGFNI
                     TDVVNGTKTFDELADEMLDSLKNSVIVTDEKSSFSNWLAPSVSVGASYNITKSLGVGV
                     LSRTLFEKSGLREMVTLSANLNVGSAFSTSISYTATGYSYDNFGAGMAFKAGFLQFYV
                     LADRIPVIWNKIIKDDVKIPLPENWNVIGLRFGVNMAIGNNVKKKSDRPMLKVE"
     gene            1356..1571
                     /locus_tag="PLG42_04445"
     CDS             1356..1571
                     /locus_tag="PLG42_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30495.1"
                     /translation="MIRIVLFVIIIILILRILLLSRMENKRRISNRWYSETDFKKVFG
                     EKNFRKSLQGDTVNKKARKSKKKIISE"
     gene            complement(1558..2307)
                     /locus_tag="PLG42_04450"
     CDS             complement(1558..2307)
                     /locus_tag="PLG42_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU30496.1"
                     /translation="MITVKNLYKSFNGKEVLTDISVTFEPGKTNLIIGQSGSGKTVLL
                     KSIVGLHEIDRGEIWFGDVPFNKLGFREKKEIRKQMGMLFQGSALFDSMTVEQNVRFP
                     LDMFTNMTMKEKIKRVNFCLDRVNIHNANHLYPSELSGGMRKRVAIARAIALNPKYLM
                     CDEPNSGLDPITANLIDALIKEITEEYSMTTIINTHDMNSVMEIGEKIIFISEGIKYW
                     EGSKYEILNSTCEKLNDFVFANSLAKQLIRK"
     gene            complement(2304..3050)
                     /locus_tag="PLG42_04455"
     CDS             complement(2304..3050)
                     /locus_tag="PLG42_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU30497.1"
                     /translation="MDRGLETIGRYFILLGKVFSKPEKTSVYYKQTMRELVYLGVNSI
                     GITAIISFFMGAAITLQTAYNTENPIYPAYLIGLGCRDSIILEFSSTIVALILAGKVG
                     SNIASEIGTMRVTEQIDALEIMGVNSASYLILPKMIATLLFNPFLTLLSIIIGIFGGW
                     FVGTQIVGVITSEQFIYGIQYAFIPYYITYSLIKTVVFAFLITSISAYQGYFVKGGSL
                     EVGQASTKAVVYSSVYILMFNVILTQMLLT"
     gene            3182..4267
                     /locus_tag="PLG42_04460"
     CDS             3182..4267
                     /locus_tag="PLG42_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOU30498.1"
                     /translation="MNNRYVIIMAGGIGSRFWPLSRKEKPKQFLDILGRGETLIQQTF
                     RRFKKVCPEENIFVVTNVEHRQIAIDQLGINPGRVLSEPLRRNTAPCIAYGVFRIKKE
                     NPDAVIAVTPADHLIEKEEEFYNIVNIAYSFSEKHNALLTLGIKPSRPETGYGYIQAN
                     EKKPVEGFEKLHKVKTFTEKPNLELAKVFLESGDFYWNSGIFFWSVNSILKAFSKYLP
                     DIYMAFEEGQHFLGTEKEESFISRTYISLPSISIDYGIMEKADNVYVLSTDIGWSDLG
                     AWSSLYEHSVTDENGNVNVNGHLFIYSSKRNIFNIKPGKVAVIQGLEDYIVVDSGDVL
                     LIVKKEDEQNIKYYLDDVKKKTGDKFL"
     gene            complement(4335..5180)
                     /gene="gldN"
                     /locus_tag="PLG42_04465"
     CDS             complement(4335..5180)
                     /gene="gldN"
                     /locus_tag="PLG42_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOU30499.1"
                     /translation="MSAMKRMIICGFLAVIFSSLVNAQTFKDIYQKSIPDNPKINYPF
                     LREADVFWSKYIYRVIDLRERINHPLYFPTETTPDGRKSFINIILDEIKAGRINAYDW
                     TIVPDSTPVSLTYADIEEKMGARLINTQIQDINTQMLKDTVIQQKANPADVKQLLIYE
                     QWFFDKKHSKLDVRIIGICPIHIRLDEATGRTVRTPVCWLRYDELRDILAKKEVFNPF
                     NDSQRLSYDDLFMYRRFGSYIYAESNVFNDRTITSYTTGKDAMFEAERIKMEIFNFEH
                     DLWEY"
     gene            complement(5201..6859)
                     /gene="gldM"
                     /locus_tag="PLG42_04470"
     CDS             complement(5201..6859)
                     /gene="gldM"
                     /locus_tag="PLG42_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOU30500.1"
                     /translation="MAHEKLSPRQKMIGMMYLVLTAMLALNVSKEAVEAFKNVDKGLS
                     KTIQNYAAKNSTIYEAFDRAAAQNPVKAGPARDKAYMVKQRSDELFDYIQDLKIEIIK
                     KTEGDDTPAVVGREIIIDEVKKIDQRDVPSEVLIGAHEAGKGNDLKALIDSYREDMIR
                     DVLEGKNPNLEESLRNIFNTDDVTTKEGEKVPWVNDNFQTLPVVAVICILSKMQVDVR
                     NAETDLLNYLYEQIEARSYKFNKLVATVKPSSTYVMQGNDYTAEVFLTAVDTTQTPSI
                     TVGEYRTTTAADGSVKYEMVGDYVTLPIDQSGKGIYRVRATSIGQKTWGGLITIKAPD
                     GTLVSYPFKESYSVGAPNVVISPTAMNVLYANIDNPIDISVPGVGSDKIRATMKNGTI
                     ARGRTKNSRGEFFPGEWIARPEVVGQNAQIIVSAEISGKIQSFAPMEFRVKAIPKPVA
                     KFANVTGNASLSKDIILAQQAVFADLEGFDFDLRYNVTEFRMTYNEKGFDIYRDSKSN
                     RITPEQRQILNSLTRGKKLYIENIKAVGPDNRPQDLSPIIITVN"
     gene            complement(6902..7786)
                     /gene="gldL"
                     /locus_tag="PLG42_04475"
     CDS             complement(6902..7786)
                     /gene="gldL"
                     /locus_tag="PLG42_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOU30501.1"
                     /translation="MSLAELVQSSGWKNFIAKLYGLGASVVIIGALFKIQHWPFAGTM
                     LTVGLCTEAIIFFFSAFEPLREEVDWSLVYPELAGIPEEGEEVPVGGKMHDPYFQRKG
                     LVGGGGAGSAALAKFDEMLEKAEITPELFQNLGLGLKKIGEATANMNAMLDVSAASEK
                     YVQTIKAADESLGKLHESYENTAKIINETGLNYKNIAESLSVIEMGGKSYQQQLETLN
                     KNLSALNAAYELQLKGATEHVKESENIYKGIQNLLKNLADTADDTKKYREQISKLNEN
                     LSALNNVYGNMLATMNVK"
     gene            complement(7823..>8784)
                     /locus_tag="PLG42_04480"
     CDS             complement(7823..>8784)
                     /locus_tag="PLG42_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU30502.1"
                     /translation="LNWDEKIDLRDEQTLAIREEMYYPPQERFNGRKEIDTRKLNYSY
                     YFVDYKQAAQVPYIQDYKAGTGKYVGEAYNLEGVRTPVNDRSAFIIHDIINVYPDTLC
                     WIRDFTYSFNDPQASIYFWHPSFDDYPVVGVSWVQARAFSIWRTDYMNSFLRKRGIPD
                     VQAYRLPTEGEWEYAARGGLDLSMYPWGGPYTRNYQGCFLANFKPLRGNYIDDGSVYP
                     AKVGSYEPNEYGLYDMAGNVSEWTSTAYHPVSYVFTHDLNPNYEYNARPQDPPVMKRK
                     VIRGGSWKDIAYFLQTSSRDYEYQDSTKPYIGFRNVRSYIGVK"
BASE COUNT         2462 a         1664 c         1834 g         2824 t
ORIGIN      
        1 ctgaatcctg cactcagacc ttcaaacaca ttttatctcg gactcggaat aacttgcata
       61 ggaataggca taaacaataa ttactttaat ttttctgacg ttatcatgca aggacgttcc
      121 gattcactga taacttttct tcatcccgat tatgatgttg acaaattcct tggtaaagtt
      181 aaggcaggta attttttaac cccgcaggca gatgttcagt tgtttggtct tggcttcaat
      241 acagggaaag atacatatat ctttctggat ctgattgaga gagtaaaggg aaatatcact
      301 cttccaggag acctaataag aattatgctt aaagggaatg aagaatttgt aggcaaaaca
      361 atggatctgg gagctctcga tgcaagtctg atgtatttca gggaagcagg gattggtttt
      421 tcagcaccag ctgggaagag agccagattt ggaataaggg ctaaagcact tttcggtatt
      481 gcaggaatct cgctcgacaa caaatttctt tcgattactg taaatgacga ctatacccat
      541 attttcaatg ctgatctgac agcaaatatt agcggaccgg tgaccgtata tttcggtgct
      601 gacaacaaac ctgacagtct ttctgttgac gaggataaac tgaagtcgaa agatttttat
      661 ctcaacacaa ggaatgcagg actgggactt gatattggag cagttgtcaa cattacagac
      721 cgtttgagtg tatcagccgc tgtcacagac cttgggtttc tgaggtggaa tgacatgata
      781 acaaatatta aggcagaaag ccagttcact tttagcggat tcaatatcac agatgtagtt
      841 aatggcacaa aaacatttga tgagctagct gatgaaatgc tcgactctct aaaaaattca
      901 gtgatagtaa ctgatgaaaa gagctctttc tcaaactggc tggctccttc tgtaagtgtt
      961 ggagcaagct ataatatcac aaaaagtctg ggagtaggtg tactctcccg cactttgttc
     1021 gagaaaagcg ggcttagaga aatggtaact ttatcagcca acctgaatgt tggaagtgca
     1081 ttttccacaa gtataagtta cactgccaca ggttacagtt acgacaattt tggagcagga
     1141 atggcattta aagctggatt cctccagttt tatgttcttg ccgacagaat tccggttata
     1201 tggaataaaa taatcaagga tgatgtgaaa atcccactcc ctgaaaactg gaatgttatt
     1261 ggattaaggt tcggtgtgaa tatggctatt ggaaataatg ttaaaaagaa aagtgacagg
     1321 ccgatgctga aagtcgaata atgaaataag tatttatgat aagaatagtt ctttttgtaa
     1381 taattataat tctgattttg agaatactat tgctttcgag aatggaaaac aaaaggcgta
     1441 tctcaaacag atggtattcc gagactgatt ttaaaaaagt atttggagaa aaaaatttcc
     1501 ggaaaagtct acagggagat actgttaata aaaaagccag gaaaagcaag aagaaaatta
     1561 tttccgaata agctgttttg ccagtgagtt ggcaaataca aaatcattca gtttttcgca
     1621 ggtggaattg agtatttcgt atttgcttcc ttcccagtat tttatcccct cgctgataaa
     1681 gataatcttt tctcctattt ccatcaccga attcatgtcg tgagtattta ttattgttgt
     1741 catagaatat tcttcggtaa tttcttttat cagggcgtct atcaggttgg cagttatggg
     1801 gtcgaggcct gaatttggtt catcgcacat aaggtatttt ggattaagag ctattgctct
     1861 tgcaattgct acacgttttc tcatcccgcc tgaaagttca gaggggtaaa ggtggttggc
     1921 attgtggatg ttgactctat cgagacaaaa atttactctt tttatttttt ctttcatcgt
     1981 catgtttgta aacatgtcga ggggaaatct tacgttctgt tccacagtca ttgagtcgaa
     2041 aagcgcactt ccctgaaaaa gcattcccat ctgttttctt atttcctttt tctctctgaa
     2101 gcccagctta ttaaaaggaa catcgccgaa ccagatttca cctctgtcta tttcgtgcaa
     2161 tccgacgatt gacttaagaa gtacggtttt ccccgagccg ctctggccta ttatcagatt
     2221 ggttttgccc ggttcgaaag taactgagat gtcggtcagt acttctttac cgttaaatga
     2281 cttataaagg ttttttactg taatcatgtc agaagcatct gagtaagaat aacattaaac
     2341 atcagaatat atacgctgct gtaaaccaca gctttagttg acgcctgtcc tacttccaga
     2401 gatcctcctt ttacaaaata accctggtat gccgagatac ttgtaataag aaaagcaaag
     2461 acaacggttt taataagtga gtatgttata taataaggta taaaggcata ctgaattccg
     2521 taaatgaatt gttccgaagt gattactccc actatctggg ttccaacaaa ccatcctccg
     2581 aatatgccta ttattatact cagcaatgta agaaaaggat tgaacaaaag tgtcgcaatc
     2641 atcttgggca gtatcaggta agaagccgag tttacaccca taatctcaag agcatcaatt
     2701 tgttctgtaa cacgcatagt gcctatttct gatgctatgt ttgaacccac tttccccgcc
     2761 agaataagtg caactattgt tgaggagaat tcaagtataa tagaatcgcg gcatccaagg
     2821 ccaatcaggt aagccgggta aatagggttt tcggtgttat atgctgtctg cagtgtgatg
     2881 gcagctccca taaaaaaaga gataatagct gttataccta tggaattgac gcctaaataa
     2941 accagttcac gcatggtttg cttgtaatag actgatgttt tttcaggctt tgaaaagact
     3001 tttccaagca gtataaaata cctacctata gtttcaaggc ctctgtcaat taacatagtg
     3061 ccattcctgt tatgaaactt caaagttaaa aattattgtc aattaattcc gggtgttctg
     3121 gttctttaga ctgaatgtgc aaatagtttt atctttgcat taaaacataa accggtcaga
     3181 gatgaataac agatatgtta taataatggc tggcggcatc gggagcaggt tctggcctct
     3241 gagccgcaaa gagaagccga aacagtttct tgacattctt gggagggggg aaacactaat
     3301 tcagcagaca ttcaggcggt tcaagaaggt atgtccggaa gaaaatattt ttgtggtaac
     3361 aaatgttgag cacaggcaga ttgctattga tcagcttgga attaatcccg gcagggttct
     3421 cagcgagccg ttaaggcgca atactgcacc atgtatagcg tatggggttt tcagaataaa
     3481 aaaggagaat cccgatgcag taatagctgt aactcctgct gaccatctta ttgaaaaaga
     3541 agaagaattt tacaacattg tgaatattgc atatagtttt tcagaaaaac ataatgcgtt
     3601 gttgacttta ggaataaaac ccagcaggcc agaaacagga tatggatata ttcaggcaaa
     3661 tgaaaaaaag cctgttgaag gttttgaaaa gttgcataag gttaaaacgt ttactgagaa
     3721 gccgaatctg gaacttgcaa aggtctttct tgaaagcgga gatttttact ggaattccgg
     3781 aatttttttc tggagcgtaa attcaattct gaaagccttt tcaaaatatc ttcccgacat
     3841 ttacatggct tttgaggaag ggcagcattt tctgggaaca gaaaaggagg aatcatttat
     3901 atcccgtact tatatttcgc tcccaagtat atcaattgat tacgggatta tggagaaagc
     3961 cgataacgtt tatgttttaa gtactgatat aggctggtct gacctcggcg cctggagttc
     4021 cctttatgaa cattctgtaa ctgacgagaa tggaaatgtg aatgtcaacg gacatctgtt
     4081 tatttatagc agcaaaagga acatttttaa tatcaagcca ggaaaggttg ctgtaattca
     4141 gggtttggaa gattatatcg ttgtcgattc aggggatgta ctattgatag taaaaaagga
     4201 agatgagcaa aatattaaat actatcttga tgatgtaaag aaaaagacag gagataagtt
     4261 tctatagaaa ataaaaaggg ggcatttgcc cctttttttg tttatacagt tatatggttt
     4321 ataaagttct ctgattaata ttcccaaagg tcatgttcga aattgaaaat ctccatttta
     4381 attctttcag cttcgaacat tgcatctttg ccggtagtat atgatgttat tgtacggtcg
     4441 ttgaacacat tggactcggc atagatatag cttccgaatc ttctgtacat gaagaggtcg
     4501 tcatatgata atctttgtga gtcgttaaac ggattgaaaa cctctttttt ggcaagtata
     4561 tcccttagtt catcgtatcg cagccagcag acaggcgttc taacggttct tccggttgct
     4621 tcgtcgagcc tgatatgtat tgggcatata ccaataattc ttacatcgag ttttgaatgt
     4681 tttttatcga agaaccattg ttcatagata agaagttgtt taacgtcagc aggatttgct
     4741 ttttgctgga ttacagtatc tttcagcatc tgtgtgttga tgtcctgaat ctgggtattt
     4801 attagcctgg cacccatttt ttcttcaata tcggcataag taaggctcac cggcgtggag
     4861 tcaggcacaa ttgtccagtc ataggcatta atcctgccag cctttatttc atcaagaatt
     4921 atattaataa aacttttcct tccgtcagga gttgtttcag tagggaaata gagcggatgg
     4981 ttgattcttt ccctgaggtc tatgaccctg taaatgtatt tcgaccagaa cacatcagct
     5041 tcccttagga aagggtagtt tattttcgga ttgtcaggaa tagatttctg ataaatgtct
     5101 ttgaaggtct gggcatttac cagtgagctg aatattacag ccagaaatcc gcaaataatc
     5161 attcgcttca ttgcactcat atgttaagtc tgttaggttt ctaatttaca gtaataatta
     5221 taggtgaaag gtcttgtggc ctgttatcag gtccaactgc ttttatattt tctatatata
     5281 attttttgcc gcgcgtaagt gagttaagga tctgtctctg ttcgggagtt atacggttgc
     5341 ttttgctatc cctgtaaata tcaaatccct tttcattata agtcattctg aactcggtaa
     5401 cattatatct gaggtcgaaa tcgaaccctt caagatctgc aaacactgcc tgttgtgcca
     5461 gtataatatc cttgctaagt gatgcgttac cggtaacatt ggcgaatttt gccacaggtt
     5521 tcggtatagc cttaactctg aattccatag gagcaaaaga ctggattttc ccgcttattt
     5581 cggctgatac aatgatctgg gcgttctggc ctacgacttc ggggcgagca atccattcac
     5641 ccgggaagaa ttcacctctt gaatttttgg tacggcctct tgcaatggtt ccgtttttca
     5701 ttgttgccct gattttgtcg gaaccgaccc ctggtacaga gatatcaata gggttgtcaa
     5761 tatttgcata cagcacattc attgcggttg gagagataac aacgttagga gcgccaactg
     5821 aataagattc tttaaacgga tatgaaacaa gagtaccatc aggggctttt attgttatca
     5881 aacctcccca tgttttctga ccaatgcttg ttgctcttac tctgtaaatt cctttgcccg
     5941 attgatcgat agggagggtt acgtaatctc ctaccatctc atattttact gaaccgtctg
     6001 cggctgttgt agttctgtat tcgccaacgg ttatactggg agtctgtgtt gtatcaacag
     6061 cggtgagaaa tacttctgca gtataatcat ttccctgcat tacatatgtt gaagaaggtt
     6121 taacagtggc taccagttta ttgaatttgt acgaacgtgc ctcaatctgt tcatagaggt
     6181 aattgagcag gtcggtttca gcatttctga catccacctg catttttgac aggatacaga
     6241 ttactgctac aacagggagg gtctggaagt tatcatttac ccagggtact ttttcacctt
     6301 cctttgtcgt aacgtcgtcg gtattaaaaa tgtttcttaa tgattcttcc aggtttggat
     6361 ttttaccttc aagcacgtct ctgatcatgt cttccctgta ggaatcgata agtgctttaa
     6421 ggtcgtttcc ttttcctgcc tcatgggctc cgattagaac ttcagaaggg acatctctct
     6481 ggtcgatttt tttaacttcg tcgattatta tttctcttcc gacgactgca ggcgtatcat
     6541 ctccttcggt tttcttaatg atttctattt tgagatcctg tatataatcg aagagttcgt
     6601 cggatctctg ttttaccata tatgccttat ctcgtgcagg tcctgctttg acaggatttt
     6661 gtgcagcggc cctgtcgaat gcttcgtaaa tggtgctgtt ctttgcggca tagttttgga
     6721 ttgtctttga cagacctttg tcaacatttt taaatgcttc cacggcctct tttgatacgt
     6781 tcagagccag catagcagtt agaaccaggt acatcatccc gatcattttt tgccgtggag
     6841 acagcttctc atgagccatt tttctgcagt tttaatttgt tgaataaaaa actatgtcac
     6901 gttatttcac attcatggta gcaagcatat tcccataaac gttattcagg gctgatagat
     6961 tttcgttcag tttagaaatc tgttcgcggt attttttagt gtcatcagct gtatcagcta
     7021 gatttttaag aagattctga attcccttat aaatgttctc ggattctttt acatgctcgg
     7081 ttgcgccttt gagctgaagt tcatatgcgg cgttaagagc agaaagattc ttgttaaggg
     7141 tttcaagctg ctgctggtat gattttcctc ccatttcgat aaccgacaat gattcggcta
     7201 tgtttttata gttcaggcca gtctcattga taattttagc cgtattctcg taggattcat
     7261 gaagcttgcc gagtgattca tctgcggctt taatagtttg aacatatttt tcagatgctg
     7321 ctgaaacatc cagcatggca ttcatattgg cagttgcttc tccgattttt ttgagtccca
     7381 gaccgaggtt ctggaacagt tcaggagtaa tttctgcctt ttcgagcatt tcgtcgaatt
     7441 ttgccagtgc tgcagatccg gctccgccgc ctccaacaag gccttttctc tggaagtatg
     7501 gatcatgcat ttttccacca acaggaactt cttctccttc ttcaggaatt ccggccagtt
     7561 cagggtaaac aagtgaccag tcaacctctt cacgtagagg ctcgaaagcc gagaagaaga
     7621 agataatagc ctcggtacaa agccctacgg taagcattgt acctgcaaat ggccagtgct
     7681 ggattttaaa aagagcacca atgattacta cggaagcacc caaaccataa agtttagcaa
     7741 tgaagttctt ccatccgctg ctttgaacta attctgctag gctcatatac ctgattttta
     7801 tttgttaata aagataatta ttttatttta ctccaatata tgatcttaca tttctgaaac
     7861 ctatatatgg ttttgttgaa tcctgatatt cataatctct tgagcttgtc tgcaggaagt
     7921 atgcaatatc cttccatgaa ccacctctta taaccttgcg tttcataacc ggtggatcct
     7981 gtggtcttgc attgtattca tagttaggat tgaggtcgtg ggtgaagaca tatgatacag
     8041 gatgatatgc agtggatgtc cattcggaga catttcctgc catatcgtaa aggccatatt
     8101 cgttgggttc atatgatcca acctttgccg ggtatactga accatcgtcg atgtagtttc
     8161 ctctaagtgg tttaaagttt gcaaggaagc atccctgata gttacgtgta tagggtccac
     8221 cccatgggta catggaaaga tcgagtccgc ctcttgctgc atattcccat tctccttcag
     8281 tgggaagcct gtatgcctgg acatcgggta tacctctttt cctgaggaat gaattcatat
     8341 agtctgttct ccagatgcta aatgctcttg cctgaaccca cgatacccct acaacaggat
     8401 agtcgtcgaa tgacggatgc cagaaataaa tagaagcctg gggatcattg aatgagtagg
     8461 tgaagtccct tatccagcac agagtgtcgg gataaacatt tataatgtcg tgaatgataa
     8521 aagctgaacg gtcgtttaca ggagtgcgta ctccttcaag attgtatgct tcacctacat
     8581 attttccggt tccggcttta taatcctgaa tataaggtac ctgggcagcc tgtttatagt
     8641 caacaaaata gtatgaataa ttaagttttc ttgtatctat ttcttttctg ccattgaatc
     8701 tttcctgtgg cggataatac atctcttcgc gtattgcaag agtctgttcg tcgcgtaagt
     8761 ctattttttc atcccagttg agag
//
LOCUS       DAOSNB010000073         8779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7969_length_8779_cov_4.392824, whole genome shotgun sequence.
ACCESSION   DAOSNB010000073 DAOSNB010000000
VERSION     DAOSNB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7969_length_8779_cov_4.392824"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLG42_04485"
     CDS             complement(<1..249)
                     /locus_tag="PLG42_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU30503.1"
                     /translation="MNFSRLWEKDVTPDPAIIEFTAGKEKQTDLLIAGWDIVGSMAHV
                     IMLHEVKLISDEEKHNLLNALHALFQKYLNGELKIGEGV"
     gene            complement(260..1492)
                     /locus_tag="PLG42_04490"
     CDS             complement(260..1492)
                     /locus_tag="PLG42_04490"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOU30504.1"
                     /translation="MKEKVVLAYSGGLDTSVILKWLIEKGYEVIAFIADVGQQEDFEE
                     CKKKALILGASKVVIRDVKREFVTDYVFKALKANAIYEDRYLLGTALARPLIASKQIE
                     VANEEGANTVAHGATGKGNDQVRFELCYYALKPGIKIISPWKDPEFLQSFKGRTDLLK
                     YAAEKNIPVKATLAKPYSEDENLMHISHEAGILEDPMFAVQKSMLVKMVMPQDAPDRE
                     TRIVITFKDGIPVKVLNKDDGTVKEDPLELFVYLNKLGGENGIGLLDMVENRFVGIKS
                     RGVYETPGATILFTAHRDIEGIAMDREVMRLRNMLTPKFSELIYYGFWFSPEMDFLMA
                     AIDKSQELIDGCVHLCLYKGNVLIEGRESPSSLYDKDLSSMDIEGGFDQTDSKGFIKI
                     NAIRLMAHRAIIESRKKK"
     gene            complement(1520..1993)
                     /gene="argR"
                     /locus_tag="PLG42_04495"
     CDS             complement(1520..1993)
                     /gene="argR"
                     /locus_tag="PLG42_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOU30505.1"
                     /translation="MQRISRLIAIENIIRSEKVSSQEEIIRKLGKMGIKCTQATLSRS
                     LKQLGVVKVPDGRGGYRYSFVSDHVPQIQAEAKYDLVNVIRSIIEVKGMILIKTSPGY
                     ANSVAITIDNYGRYEIAGTIAGDDTLLVIPRDNVSPAHVRNILEMIFPGIHSMTG"
     gene            2080..5298
                     /gene="carB"
                     /locus_tag="PLG42_04500"
     CDS             2080..5298
                     /gene="carB"
                     /locus_tag="PLG42_04500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOU30506.1"
                     /translation="MKDKLNKVIILGSGALKIGEAGEFDYSGSQALKALREEGITTIL
                     VNPNIATIQTSENIADKVYFLPVNPYFVEKVIAREKPDGILLSFGGQTALNCGIELFK
                     NKVFEKYNVRVLGTPVTAIIDTEDRELFVKKLNEINVKTPRSIAVSTVEEAVEAAGKL
                     GYPVIIRSAYTLGGQGSGFAYSAEEMETLAARALSFTGQILVEESLKNWKEVEYEVVR
                     DCYDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNNEYHLLRELSIKIIRHIGIVGE
                     CNIQYALDPNSEDYRVIEVNARLSRSSALASKATGYPLAFVAAKLAIGYGLHELKNSV
                     TKTTPACFEPALDYIVCKIPRWDLNKFTGVSHHIGSSMKSVGEVMAIGRTFEEAIQKG
                     LRMIGLGMHGFTGNRDIYFPDVEKELAEPTDMRIFSIAQALDENIPIDRIHQLTGIDR
                     WFLYKLKNIIDIKNRISVFKNIEEIPYDILLEAKKAGFSDFQIARHVLNSSSKDMNRE
                     MMRVRAFRKALGIKPYVKQIDTLAAEYPAVTNYLYLTYSGTEHDINFENDGRSVIVLG
                     SGAYRIGSSVEFDWCSVNAINTIRNAGYRAVMINYNPETVSTDYDICDRLYFDELSFE
                     RVMDVIELEHPMGVIVSMGGQIPNNLAMRLHLANVNILGTSPLSIDRAENRHKFSSML
                     DTLKIDQPRWKELTTTDEIHNFVDQTGFPVLVRPSYVLSGSAMNVVSNKNELDHFLNL
                     ATRISKQYPVVVSEFIENAKEIEIDAVAREGEIIVYAISEHVEFAGVHSGDATMVFPP
                     QRLYFETVRRIKNISREIARELNITGPFNIQFLAKDNEIKVIECNLRASRSMPFVSKV
                     LKINLIDIATKVMLGIPVEKPHKSAFDLDYVGVKAPQFSFSRLAKADPILGVDMASTG
                     EVACLGVGFYEAILKAMFSVGYRIPSKGILLSTGPAKSKAELLDAARALKEKGYKLYA
                     TRGTQQFLENAGVEAEVAYWPDEDRSPNAIELIRKGMVDMVINIPKNLSQAELSNDYS
                     IRRSAVDYNVPLLTNTRVANAFIRAFCRLSMDDLAIKSWNEY"
     gene            5400..6494
                     /locus_tag="PLG42_04505"
     CDS             5400..6494
                     /locus_tag="PLG42_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU30507.1"
                     /translation="MFWLLLIPFFIYLVLFVTAALYLRNPAVWTYNPLLQPVSVSIIV
                     PCKNEESSLPSLLADLSLQNYPEDSFEIIVVDDHSSDNTLIVAEEFSGIRNLKVISNA
                     GNGKKHAIRTGVQSSSSELIITTDADCRVGKVWVGTIASFYAACRPALIIGPVKLKER
                     KGVLFVFQHIEWLGLQGITAGYALAGRPVMCNGANLAFTRKTFMENCEDLNYTIPSGD
                     DIFLLHSVKSKQGSNILWLNSREADVATEPAYSIQELLKQRARWISKAGSYRDAFTIF
                     LSAVVFITVSLLGLMLVAALLNIKFLPFFLAGFTIKSFADALLIRTTANLYKQKSFLR
                     WLFPLQLLYPLYLAAVLIRLIPGLFSRGKL"
     gene            complement(6561..8753)
                     /gene="recQ"
                     /locus_tag="PLG42_04510"
     CDS             complement(6561..8753)
                     /gene="recQ"
                     /locus_tag="PLG42_04510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOU30508.1"
                     /translation="MGKVPDDSVIHGYLKKYFGFDTFKGLQEPIIKNVLAGRDTFVLM
                     PTGGGKSLCYQLPAVMLEGTAIVISPLIALMKNQVDAMRNFSTTDEVAHFLNSSLSKS
                     EINKVKKDVVTGITKLLYVAPESLTKEENIEFLRNIKISFYAVDEAHCISEWGHDFRP
                     EYRRIRPIIDTIGRSPIIALTATATPKVQHDIQKNLGILEADVFKSSFNRPNLYYEVR
                     SKVDATKEIIRYIKNNQGKSGIIYCLSRKKVEELAETLKVNGIRALPYHAGLDTATRT
                     ANQDKFLMEEADVIVATIAFGMGIDKPDIRYVIHYDMPKSLEGYYQETGRAGRDGGEG
                     RCIAYYSYNDIQKLEKFMQGKPVAEQEIGKQLLLEVVSYAESSVCRRKSLLHYFGEEY
                     PKENCEACDNCLHPKVQFEGSEYVVSVLEAVLAVKEKFKADHIANILAGKATSAIKSY
                     KHHKLDIFGAGEEKDEKFWNMVIRQALIAKLLTKDIENYGLLKLTPKGREFLEKPTEF
                     MLVEDHDYSDAEEEEGGGFGAKTAAVDEELFSILKDLRKKISKQKNVPPFVIFQDPSL
                     EEMAIQYPITLDELQNISGVGAGKAQRYGKEFVEVIKKYVEEKEIIRPLDMVVKSVVN
                     KSGLKVYIIQSIDMKRPLEDIAEAKGLDMDELLTEIEAIVNSGTRINLDYYIDMVMDE
                     ERQQEIYSYFKEEAQSDSLEEALQALGNEFEEEEIRLIRIKFLSEMGN"
BASE COUNT         2504 a         2076 c         1679 g         2520 t
ORIGIN      
        1 aacaccttcc cctatcttaa gctcaccatt cagatatttc tgaaaaagag catgtaatgc
       61 atttaataga ttatgctttt cttcatctga aatcagcttc acctcatgaa gcatgataac
      121 atgtgccatt gaacctacaa tatcccagcc ggcgatcaaa agatcggtct gtttttcctt
      181 tcccgcagtg aattcaatta ttgccgggtc gggcgtaaca tctttttccc acaatcttga
      241 aaaattcata tgcgtgaatt tattttttct ttctcgactc aataatagcc ctgtgtgcca
      301 taagccttat ggcatttatt ttaataaagc ctttactgtc ggtctgatca aagccccctt
      361 caatatccat gctggaaaga tctttgtcgt aaagcgacga aggtgactct ctaccctcaa
      421 taagtacatt ccccttgtaa aggcatagat gaacacaccc gtcaatcagt tcctggcttt
      481 tatcaatagc agccataaga aaatccatct ccggactgaa ccagaagcca taataaataa
      541 gttctgaaaa cttgggagtt agcatatttc ttaacctcat aacttccctg tccatcgcta
      601 ttccttcaat gtctctgtga gcggtgaaca atatagtagc ccccggagtt tcatatacac
      661 cacgtgattt tatacctaca aaccggtttt caaccatatc gagcaacccg atgccatttt
      721 cccctcccag cttgttcaga taaacaaaaa gttccagcgg atcctccttc acagtaccat
      781 catccttgtt gagaacctta accggtatcc catccttaaa tgtgattaca atgcgagttt
      841 cacgatccgg cgcatcctga ggcatcacca tcttcaccag catgcttttc tgcaccgcaa
      901 acatgggatc ttcaagaatg cctgcctcat gacttatgtg catcaggttt tcatcctcac
      961 tataaggttt ggctagcgtt gccttaaccg ggatattctt ttcagctgca tattttaaaa
     1021 ggtctgttct tcctttgaaa ctctgaagaa attccggatc cttccatggt gaaattatct
     1081 ttatacctgg cttgagagca taataacaca gctcaaaacg aacctgatcg ttacccttcc
     1141 ctgtggcacc atgagccaca gtgttggctc cttcctcatt tgcaacttca atctgcttac
     1201 ttgcaataag aggccttgca agagctgttc ccagcaggta cctgtcttca tatatagcat
     1261 tcgccttcag agctttaaat acgtaatcgg taacaaattc cctttttaca tcccttataa
     1321 ccacttttga tgcaccaagt atcaatgctt tctttttgca ctcctcaaaa tcctcctgct
     1381 ggcctacatc tgcaataaag gcaatcacct cataaccctt ctcaattaac catttaagta
     1441 taaccgaggt gtcaagacct ccactgtaag caagaacaac tttttctttc atctgaatct
     1501 gtttttatgt tattaataat tatccagtca ttgaatgaat tcccggaaaa atcatctcca
     1561 gaatattcct tacatgtgcc ggcgatacgt tatcccgggg aataacaagc agtgtgtcgt
     1621 cccctgcaat cgttccagca atctcataac gcccgtaatt gtcaatagtt atcgccacac
     1681 tgttcgcata acccggactt gtcttgataa gtatcatccc ctttacttca attatgcttc
     1741 tgataacatt tacaagatcg tactttgcct cagcctgaat ctgtggaaca tggtcactaa
     1801 caaaagaata cctgtaccca cctcttccgt caggaacttt aacaactcca agctgtttta
     1861 gacttctcga aagagttgcc tgggtacatt taatacccat cttcccgagc ttccttatta
     1921 tttcctcctg agaagaaacc ttttcgctcc ggattatatt ctctatagca ataaggcgtg
     1981 aaattctttg catttttata cattctggtt gcaaatttat tcattttttt catttctcca
     2041 cttttaaaaa gaaaaaaagt gttattttcg ccgtctgaaa tgaaagacaa gctaaacaaa
     2101 gtaataatat tagggtcggg cgctcttaaa ataggtgaag ccggtgaatt cgattattca
     2161 ggatcacagg cattgaaagc cctgagagaa gaaggaataa caactatcct ggttaatccc
     2221 aatattgcta ccatccagac ctcagaaaac attgccgaca aagtatattt cctgccggtc
     2281 aatccgtact ttgtcgaaaa agttatagct cgcgaaaaac ccgatggtat tcttttgtct
     2341 tttggaggcc agacggctct taactgcgga attgaactat ttaaaaataa ggtcttcgaa
     2401 aaatacaatg tcagggtact cggcacccct gtcacggcaa ttattgacac tgaggacaga
     2461 gagcttttcg taaagaaact taatgaaata aatgtaaaaa caccacgcag tattgcggtt
     2521 agcactgttg aagaagcagt tgaagctgcc ggaaaacttg gctatcctgt tatcatccgt
     2581 tcggcataca cacttggcgg acagggaagt ggttttgcat acagcgccga agaaatggaa
     2641 acacttgcag cccgagctct ttcattcacc ggccagattc ttgttgaaga atctctcaag
     2701 aactggaagg aagtcgaata tgaggtggtc agggattgct acgacaattg tatcacagta
     2761 tgcaacatgg aaaacttcga cccgcttgga atacatacag gagaaagcat cgtggtggca
     2821 ccctcacaga cccttaccaa caatgaatat caccttctca gggagctctc cataaaaatt
     2881 atcaggcata taggaattgt aggtgaatgc aacatacaat acgcacttga ccctaattcg
     2941 gaggattatc gggttattga agtcaacgcc cgcctttcaa ggtcgagtgc acttgcctct
     3001 aaagcaacag gatatccgct tgcttttgtg gcagcaaaac ttgcaatcgg ctacggcttg
     3061 cacgaactga aaaactcagt tacaaaaaca acccctgcat gcttcgaacc tgctctcgat
     3121 tatattgttt gtaaaatccc ccgctgggac ctcaataaat ttaccggtgt ctcccaccac
     3181 atcggaagca gcatgaaaag cgtcggtgaa gtaatggcta ttggacgcac ttttgaagaa
     3241 gcaatccaga agggacttcg gatgatcggg ctcggtatgc atgggttcac aggtaaccgg
     3301 gatatctact tccccgatgt tgagaaagaa cttgccgagc caaccgacat gagaatcttc
     3361 tcaatagcac aggcactcga tgaaaatatt ccaattgaca gaatacacca gctcaccgga
     3421 attgaccgct ggttccttta taaactgaaa aatatcatcg acattaaaaa ccggatctca
     3481 gtcttcaaaa acattgaaga aatcccgtat gatattctcc tagaagctaa aaaagccggg
     3541 ttcagcgact tccagatagc gcgtcacgtt ctgaactctt catcaaaaga catgaacagg
     3601 gagatgatga gggtgagagc attcagaaaa gctctcggaa taaaacccta tgtcaaacaa
     3661 atagacacac ttgcagcaga gtacccggca gtgacaaatt atctctacct gacttacagt
     3721 ggcacagaac atgacattaa cttcgaaaat gacggcagat cggttatagt actgggttcc
     3781 ggggcttaca ggattggctc gagtgtggaa tttgactggt gctcggtaaa tgcaataaat
     3841 actatccgca atgctggtta cagggcagtg atgatcaatt acaacccaga aaccgtaagt
     3901 acagattatg atatatgcga ccggctctat tttgatgaac tatcctttga aagagtgatg
     3961 gatgtaatcg agctcgagca tccaatgggt gtaatagtct ctatgggcgg gcaaatccca
     4021 aacaaccttg ccatgaggct ccaccttgca aacgtaaaca tcctcggtac gtctcccctt
     4081 tcaatcgaca gggcagaaaa ccgccataaa ttcagctcaa tgctcgatac cttgaaaatc
     4141 gaccagcccc ggtggaaaga acttaccaca actgatgaaa tccataattt cgttgatcag
     4201 acaggttttc cggttctcgt aaggccttct tacgtattgt caggctcggc aatgaatgtt
     4261 gtatcgaata aaaatgagct cgaccatttc cttaatcttg caacaagaat ctcaaaacaa
     4321 tatcctgtag tagtttcgga atttattgaa aatgctaaag aaattgaaat tgacgccgtt
     4381 gcccgcgaag gggaaattat cgtttatgca ataagcgaac atgtggagtt tgcgggtgtt
     4441 cactcgggcg atgcaacgat ggtgttcccg ccgcagaggc tctatttcga aactgtacgg
     4501 cgtataaaaa acatctcacg tgagatagcc agggaactaa acattacagg ccctttcaac
     4561 attcagttcc ttgcaaagga caacgaaata aaagttattg aatgcaacct cagggctagc
     4621 cggagcatgc catttgtgtc aaaagtgctg aaaataaacc tgattgacat tgcaacaaag
     4681 gtgatgctcg gtattcctgt tgaaaaacca cataaatcag cctttgacct cgactatgtg
     4741 ggtgtcaaag caccgcaatt cagcttctca aggcttgcaa aagccgatcc catactggga
     4801 gttgatatgg cttcaacagg tgaagtagcc tgcctgggag taggattcta cgaagccata
     4861 cttaaagcga tgttctccgt aggatacaga ataccaagta aaggtatcct gctttccact
     4921 ggtccggcaa aatcaaaggc cgaactgctc gacgcagcaa gagcccttaa ggaaaaagga
     4981 tacaagctgt atgccacaag aggaacacag cagtttctcg aaaatgcagg cgtcgaggcg
     5041 gaagtggcat actggcccga tgaagataga tcacccaacg cgatcgaact gatccgtaaa
     5101 ggaatggtag atatggtaat taatatcccc aaaaaccttt ctcaggctga actaagcaac
     5161 gactattcaa tcagacgcag tgcagttgat tacaatgtgc ctcttcttac caacacaaga
     5221 gtcgccaacg catttataag ggctttctgc aggcttagta tggatgacct tgctataaaa
     5281 agctggaatg aatactgaaa accggaaagc tgaaagaatt ataagaccta cgttatcgga
     5341 tttagaacaa taatgagtta ataacaaagc gaaatctgta aattgcttat tttcagtgaa
     5401 tgttctggct tcttctcata ccatttttta tatatctggt actatttgtc acggcggctc
     5461 tataccttcg caacccggct gtatggacat acaatcctct cctgcaacca gtaagcgttt
     5521 caattattgt gccctgtaaa aatgaagagt catcattacc ttcacttctt gccgacctct
     5581 cattgcagaa ctatccggaa gattcatttg aaataatagt agttgacgac cactcttctg
     5641 acaatacatt gatagttgct gaagagttca gtggaatacg caatctaaaa gttatcagta
     5701 atgccggaaa cggcaaaaaa catgctatca gaacaggggt acaatcatcc tcatcagaac
     5761 tcatcatcac taccgatgct gattgccggg tgggtaaagt atgggtcgga acaatcgcat
     5821 cattttatgc tgcctgcaga cctgccctga taataggacc tgtaaaactt aaagaaagaa
     5881 aaggagtgtt gtttgtcttt caacatattg aatggctcgg acttcaggga ataactgcag
     5941 gttatgcact cgcaggcaga cctgttatgt gcaacggtgc caaccttgcg ttcaccagaa
     6001 aaacttttat ggaaaattgc gaagacctta attacactat accgtcaggc gatgacatat
     6061 ttcttcttca ctctgtaaag tcaaaacagg gatcaaacat tttgtggctc aacagtcgtg
     6121 aagcagatgt cgcaacagaa ccggcatatt ctattcaaga attacttaaa cagagagcaa
     6181 gatggatatc caaagcaggt tcctacaggg atgcttttac tattttcctt tctgctgtag
     6241 tctttattac agtttctttg ctgggtctga tgctcgtggc agccttactg aacattaaat
     6301 tccttccgtt tttcctggca ggcttcacta ttaaatcttt tgccgacgct cttttaatta
     6361 gaactactgc taatctgtac aaacaaaaat ctttcctgag atggctcttt ccacttcaac
     6421 tactctatcc tttatatctt gcagctgtcc ttataaggct gattcctggc ttattcagca
     6481 ggggaaaatt atgagatcaa tttttactga accttgaact ttgaactttg aaccttgaac
     6541 cttgaacctt gaactttaaa tcaatttccc atttctgaaa gaaactttat tctgatcagc
     6601 cttatttctt cttcttcgaa ctcgtttcca agagcctgaa gtgcttcctc gagcgaatca
     6661 ctctgggcct cctccttgaa gtatgaatag atctcctgct gcctctcttc gtccattacc
     6721 atatctatat aataatcgag gttaattcgt gtgccggaat taacaatcgc ctcaatctcg
     6781 gtgagaagtt catccatgtc gagacctttg gcttcggcga tatcttcgag aggcctcttc
     6841 atgtcgatac tctgtatgat atagactttc agtcccgact tattgacaac agacttcacc
     6901 accatgtcga gtggccttat tatttccttc tcctctacat atttcttgat cacttccaca
     6961 aactccttgc cgtatctctg ggccttgcct gctcccactc ccgaaatatt ctgcagttca
     7021 tcaagggtaa tcgggtactg tattgccatc tcttcaaggg aaggatcctg aaaaatgacg
     7081 aaaggcggaa catttttctg ttttgatatc ttcttcctca ggtctttgag tatgctgaag
     7141 agttcctcgt caacagcggc agtttttgca ccgaaaccgc caccttcttc ttcctccgca
     7201 tcgctatagt cgtggtcttc aacaagcata aactcagtag gtttctcgag gaattccctg
     7261 cctttcgggg taagtttaag caaaccgtaa ttttctatat ccttcgtaag gagtttggcg
     7321 atgagagcct gcctgataac catattccag aatttctcgt ccttttcttc gccagccccg
     7381 aaaatatcaa gcttatgatg tttgtacgac ttaattgccg atgttgcctt ccctgcaaga
     7441 atattggcta tatggtctgc tttaaacttc tccttcactg ccagcacagc ctcaagcacg
     7501 ctaaccacat attcactgcc ctcaaactgc actttcggat gcaggcaatt atcgcatgcc
     7561 tcgcagtttt ccttcggata ctcttctccg aaataatgaa ggagcgattt acgtcggcat
     7621 accgacgact cggcatagga tacaacctcg agcaacagct gctttcctat ctcctgctcg
     7681 gcaacaggct ttccctgcat aaatttctca agcttctgaa tgtcgttata gctgtaatat
     7741 gcgatacatc tgccttcacc tccatccctt ccagcacgtc cggtctcctg gtaatatcct
     7801 tccagacttt taggcatgtc gtaatgtatt acataccgta tgtcaggctt gtcgattccc
     7861 atcccaaacg caattgtggc aacaatcaca tcagcttctt ccatcagaaa cttatcctga
     7921 ttggctgtac gtgttgcagt gtcgagccct gcatgatatg gcaacgccct tatgccgtta
     7981 actttcaaag tttcagccag ttcctcaact ttcttcctgc taaggcaata aataataccc
     8041 gatttaccct gattgttctt aatatatctg attatttctt tggtggcgtc aaccttcgac
     8101 cttacttcat agtacaggtt gggcctgttg aaagaagatt tgaaaacatc tgcctcaaga
     8161 ataccgaggt ttttctgaat atcatgctgg actttgggtg tggctgttgc tgtaagggca
     8221 attatcggtg aacgcccgat agtatcaata atgggcctta ttctcctgta ttcaggcctg
     8281 aaatcatgtc cccattccga gatacagtga gcttcatcaa ctgcataaaa agaaatttta
     8341 atgttcctca ggaattctat gttctcttcc tttgtaagtg attcgggtgc tacatataat
     8401 aatttggtaa tacctgttac aacatccttt tttaccttgt ttatctccga cttcgagagg
     8461 gatgaattga gaaaatgagc aacctcatcg gtagtgctga agttgcgcat agcatcaacc
     8521 tgatttttca tcagggcaat aagcggtgat ataacaatag ccgtaccttc cagcattact
     8581 gccggaagct gatagcagag cgacttacct cccccagtag gcataagtac aaatgtgtct
     8641 ctccctgcaa gaacattctt gattattggc tcctgtaaac ccttgaatgt gtcaaaacca
     8701 aagtatttct tcagataacc atgaatcaca ctatcgtcag gtactttccc catatctctc
     8761 tttatcacta tttaaatta
//
LOCUS       DAOSNB010000074         8759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_7997_length_8759_cov_4.061811, whole genome shotgun sequence.
ACCESSION   DAOSNB010000074 DAOSNB010000000
VERSION     DAOSNB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7997_length_8759_cov_4.061811"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PLG42_04515"
     CDS             complement(<1..1402)
                     /locus_tag="PLG42_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOU30509.1"
                     /translation="MADPTGFLKVRRKEAGNRPIHERICDFSEVEQVLNSDDRMLQAS
                     RCMDCGTPFCHWACPLDNLIPEWNDLLWRGEWKSAYNRLNATNNFPEFTGRICPAPCE
                     HACVLNINDEPVTIRENEVAISERAFTEGYIKPKPPRKRTDKRVAVIGSGPAGLAAAD
                     LLNKWGHNVTIFEKNNKAGGLLRYGIPDFKLSKVTIDRRLNLMTFEGVEIRTGTEIGK
                     DIPGRVLLSSYDALCLAIGAMKPRDLNIEGRDLNGIYFAMDFLTQQNRINDGEIIPAQ
                     ERISAEGLNVIVLGGGDTGSDCVGTAIRQKAKSVTQIEIMPMPPLKRSPNNPWPYYAK
                     ILKTSTSHEEGCERMWSLSTNRFIGENGYLTGIEVEDVVWELSGGQHIMKPLPETRRI
                     IPADMVVLALGCMHPALEGLIEELGLELDDRKNIKTDINLHTSVRGVFAAGDAVSGAS
                     LVVNAIASGRKAAMAID"
     gene            complement(1480..6003)
                     /gene="gltB"
                     /locus_tag="PLG42_04520"
     CDS             complement(1480..6003)
                     /gene="gltB"
                     /locus_tag="PLG42_04520"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HOU30510.1"
                     /translation="MKSTNRGLYRSDFEHDSCGIGFVAHIKGRKSHSIISKGLEILCN
                     MAHRGAEGADSRTGDGAGIMIQIPRDFYLIQGYALPPSGYFGTGILFLPQNSHEADEC
                     LKILGEVAAEEGVKTLGFREVPRNNSVLGELALMTEPVMKQIFMTADTEPEVLERKLY
                     IIRKITEKRIRESNLGQKDFFYIPSLSTRVLVYKGMLTSYQLKEYYLDLKDERLQSAI
                     ALVHSRFSTNTFPSWDLAQPFRFIGHNGEINTIRGNRIWMEARESILKSEALGDLSRI
                     YPIIEPGKSDSASLDNVVEFLVMSGKSLPYAMSILIPESINIKNPIPPELRDFYHYHA
                     TFMEPWDGPASLIFSDGRYIGGMLDRNGLRPSRFTITSNDLIIMASETGVQEFEPDEV
                     VAKGRLRAGKILLIDTEEGKIYYDDEIKTKLASEYPYGKWIEANQVLLEDIETGQHFS
                     PALDGDYEKYLVSFNYSLEDIEKIITEMAATGKEPIASMGHDTPLAVLSEKPYRLFDY
                     FKQLFAQVTNPPIDPIREELVMTLSGYLGSLQKNLLDTTPDHVKMVKFKIPVISNTYF
                     NIIKNLRYKGFSCSVFKMHFEVSKGAEGLKEAIEKLCYDCEKAVDEGKNYIILSDRGI
                     DREHAPIPSLLAVSAVHHYLVEKRKRMQIDIIVESAEPREVMHFALLFGYGASVINPY
                     MSFAIIERLVKDKVIQLDYKKAEENYINAINKGLLKIMSKMGISTLRSYRSSQIFEAI
                     GISRDVVDRYFKGTPSRIGGLGLEEITQEILIPHNKAFSEDSTPEIINKGIYSFRKHG
                     EHHAWNPDTIALLQWSTRAGDYSKYKEFSRRVSEENSRPTFLRGLFKLKEGNPVPLDE
                     VEPAESIMKRFVTGAMSFGSISREAHETLAIAMNRIGGRSNTGEGGEDPERFKPRPNG
                     ENARSAIKQVASGRFGVTTEYLVNADEIQIKIAQGAKPGEGGQLPGHKVDKIVAKTRH
                     SIPGITLISPPPHHDIYSIEDLSQLIFDLKNVNPRANISVKLVSECGVGTIAAGVVKA
                     KADIITISGFEGGTGASPISSIRHAGLPLELGLAETQQTLVMNNLRKRVKLQVDGQLK
                     TGLDVIIMALLGAEEFGFATAALIVLGCVMMRKCHMNTCPVGIATQNEDLRKRFTGKA
                     DYLVNYFRFVAEEVRELLAQLGYRSLDEIIGRADLLERNYEINNWKSRTLDLSPILTL
                     PPEAKVNPIKCVEKQIHNIDNVLDRELINLADKAINEKEPVLIEKKIKNTDRSVGAML
                     SGKIAGIYGQEGLPDGTIRCIFRGSAGQSFGAFLIKGIEFRLEGDSNDYLGKGLSGGR
                     IIVIPPEGSLFEPDKNIITGNTVLYGATSGEVYISGMAGERFAVRNSGAIAVVEGAGN
                     HCCEYMTGGRVVVLGSTGNNFAAGMSGGIAYVLNETGKFDYFCNMGMVELSLVEDRSD
                     VHELKGLIARHYYYTGSKKAKMILDDFDKYLEMFIKVIPYEYKKVLHEQKLEEIKKKI
                     ADLELDVEFVRGS"
     gene            6352..7878
                     /gene="zwf"
                     /locus_tag="PLG42_04525"
     CDS             6352..7878
                     /gene="zwf"
                     /locus_tag="PLG42_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOU30511.1"
                     /translation="MSNQSETLFIIIFGATGDLTYRKLAPALYSLNIQGSIPERFYIV
                     GVGRKNLTDEEFRARMREGIKLFSDKSEIRDSSIEELLEHFLYIKSDMVSHEGYTDLK
                     SLIKKKEDESGGAINCIYYLATPPDLFSVIPFRLHEAGLTSEDKGYIRFVFEKPFGYE
                     YASCRKLNEDLHRILSEKQIYRIDHYLGKETVQNILVMRFSNGLFEPLWNRNYIHHIQ
                     ITSAESIGVENRGGYYDSAGALRDMVQNHLLQMVGLTAMEPPSSIHPDAIRNEVLKVF
                     QSLQPLKEEEVPEYVIRGQYTASKVMGQCIPAYRQEPGVNPASRTETYVAMKFFINNW
                     RWGGVPFFIRTGKRLPTRVTEIVVNFKPTPHFLFHVNDMNKAINQLIIRIQPDEGIVL
                     KFGIKKPGSGFNVENVSMDFHYSDLLKVRVPEAYERLLGDVIMGDSTLFARDDIVETA
                     WKFLEPVQKAWAENRDIKIYGYPAGTWGPEEAGKIIGDGLEWRYPCKNLADDGTFCEL
                     "
     gene            7883..8620
                     /gene="pgl"
                     /locus_tag="PLG42_04530"
     CDS             7883..8620
                     /gene="pgl"
                     /locus_tag="PLG42_04530"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOU30512.1"
                     /translation="MDRIIKISNNIMALSHEVAADLSAIINNAVRRSHHLNIALSGGN
                     TPRTFYSVLGTHYALSTEWKAVDFFWSDERCVPPGDRESNYRVAREHFFERIKIQAGK
                     IHRIRGEDDPQTEAIRYSELIKKKVPFRFGFPVFDIVFLGLGTDGHIASIFPGDEKVL
                     SSVRTCEVAVHPETGQKRITLTLPVINNAERIIFLVSGKDKAEIVAEILNNPDAKHYP
                     AGMVNPHFGKIYWYMDLDAASLLSQYA"
BASE COUNT         2457 a         1876 c         1628 g         2798 t
ORIGIN      
        1 tgtcaattgc cattgccgct tttcttcctg aagcaatggc attaacaaca aggcttgcgc
       61 cagatacagc gtcgcctgca gcgaaaacac ccctcacact ggtatgaaga tttatgtctg
      121 ttttgatgtt tttacggtca tcaagttcca atcccagttc ttcaataagg ccttcaagag
      181 caggatgcat acaaccgaga gcaagcacta ccatatcagc gggtattatt cttcgtgttt
      241 ccgggagtgg tttcattata tgttgtccgc cggataattc ccaaacaaca tcttcaactt
      301 ctatacctgt taaataacca ttttcaccta taaatctgtt agttgataaa ctccacatcc
      361 tttcacagcc ttcttcgtgt gaagttgatg ttttaaggat ttttgcataa tatggccatg
      421 gattgtttgg cgatcttttc agtggaggca ttggcattat ttcaatctgg gtgacactct
      481 ttgctttttg gcgtatagca gtacccacgc agtctgaccc ggtgtcgcca cctccaagta
      541 caattacatt aagtccttca gcggatattc tttcttgagc aggaattatt tctccgtcat
      601 ttattctgtt ttgttgtgtg aggaaatcca tggcaaagta tattccatta agatcccgtc
      661 cttcaatgtt gaggtcacgg ggtttcatgg cacctattgc cagacataga gcatcgtatg
      721 agcttaataa taccctgcca ggaatatcct taccaatctc agttcctgtt cttatttcaa
      781 caccttcaaa ggtcattaaa ttaagacgtc tatcaattgt gactttactg agtttaaagt
      841 cggggattcc gtatctgaga aggcctccgg ctttattgtt tttttcaaat attgttacgt
      901 tatggcccca tttattaaga aggtctgctg ctgccagtcc ggcaggtccg ctgccgataa
      961 ctgccactct cttgtctgtt cttttacgtg gaggtttagg tttgatataa ccttctgtaa
     1021 aagctcgttc agaaatagcg acttcatttt ctctgattgt cacaggttca tcgttaatat
     1081 tcagaacaca tgcatgttcg caaggagcag ggcatatcct tccggtgaat tcaggaaaat
     1141 tatttgttgc gttaagcctg ttataggcac ttttccattc tcccctccag agcaggtcat
     1201 tccactcagg tatcaggtta tcgagcgggc atgcccagtg gcagaagggt gtgccacaat
     1261 ccatacatct cgatgcctga agcatacggt catcactgtt aagtacctgc tcgacttcac
     1321 tgaagtcgca tattcgttca tgtataggcc tgttgcctgc ttccttgcgt ctaaccttta
     1381 aaaaacctgt cgggtcagcc ataattctga tattctttta atttatatga ttacttttga
     1441 ttactttatt tattaattct cagttcagat ataacgtatt tatgatcctc tcacaaactc
     1501 cacatctaac tcaaggtcag caattttctt ctttatttct tctagtttct gttcatgcaa
     1561 aacctttttg tattcatatg gtattacttt aataaacatt tcaagatatt tatcaaaatc
     1621 atcgagtatc atttttgcct ttttactgcc tgtgtaataa tagtgtcttg caatcaatcc
     1681 tttgagttca tgtacatcgc ttctatcttc aacaagtgat agttcaacca tacccatgtt
     1741 gcagaaataa tcaaacttac cggtttcgtt aagtacataa gcaattccac cgctcatacc
     1801 tgctgcaaaa ttgttaccgg tactacccag aacaaccact ctgccacctg tcatgtactc
     1861 gcagcaatgg tttcctgctc cttcaactac agcaattgct ccgctgtttc ttacagcaaa
     1921 tctttctcct gccattcctg aaatatagac ttctccgctt gtagctccgt agagaacagt
     1981 attaccggtt attatattct tgtcaggttc gaataacgag ccttcgggag gtataacaat
     2041 aatccggcct cctgataatc ccttaccaag gtaatcattt gaatctcctt ccagcctgaa
     2101 ctctattcct ttaatcagaa aggcgccaaa gctctgacct gctgagcctc tgaatataca
     2161 tcgaattgtt ccatcaggta aaccttcctg tccataaatt ccggcaattt ttcccgataa
     2221 cattgcacct acagacctat cagtattttt tatttttttc tcgattaata ctggctcttt
     2281 ttcgtttatt gccttatctg ccagattaat cagttcccta tcaagaacat tgtctatgtt
     2341 atgaatttgt ttttctacac atttaatagg attaactttt gcctcggggg gcaatgtgag
     2401 aattggtgaa agatcaagtg tccttgactt ccagttatta atttcataat tgcgttccag
     2461 aaggtctgcc ctgccgataa tttcatcaag cgacctgtag cccagttggg caagaagctc
     2521 ccttacctct tcagctacga atctgaaata attaaccaga taatcagctt ttccggtgaa
     2581 tctttttcga agatcttcat tctgtgtcgc aatacctaca ggacatgtat tcatatggca
     2641 ttttctcatc attacacaac caagcacaat cagtgctgca gttgcaaatc caaattcctc
     2701 tgcaccaagt aaggccataa taattacatc aagacctgtt ttcagctgtc cgtcaacctg
     2761 aagtttcact cttttcctca ggttattcat taccagtgtt tgctgggtct cagcaagtcc
     2821 aagttcgaga ggtaatccgg catgtcttat tgaggaaatg gggcttgccc ctgttccgcc
     2881 ttcaaaacca ctgatggtta ttatgtcggc ctttgctttc acaactcctg cagcgatagt
     2941 cccaacaccg cattccgaaa caagtttaac actgatattt gctctggggt taacattttt
     3001 cagatcgaag attaattgag aaagatcctc aatagaataa atatcatggt gagggggagg
     3061 tgatataagt gttatccctg gaatagaatg ccttgttttt gctactattt tatcaacttt
     3121 atgtcccggc aactgtccgc cttcgccagg ctttgcaccc tgtgcgattt taatctgtat
     3181 ttcatccgca tttaccaggt attctgttgt tactccaaaa cgtccgctgg caacctgctt
     3241 aatggcgctg cgtgcatttt caccattcgg cctgggtttg aagcgttcag ggtcttctcc
     3301 tccttcgcct gtgttgctcc ttcctccaat cctgttcata gcaatagcca gagtttcatg
     3361 cgcttcccgg cttatagaac caaacgacat agcacccgtc acaaatctct tcattattga
     3421 ctcagcaggt tccacttcat caagaggcac aggattgccc tctttcagtt tgaacagacc
     3481 ccttaaaaag gtcggtcgtg aattttcttc agataccctt ctgctaaact ctttatattt
     3541 cgaataatct ccagcccttg tactccattg aagaagtgca atcgtatccg gattccaggc
     3601 atgatgctct ccatgttttc taaatgagta tataccttta tttattatct ccggtgttga
     3661 atcttcactg aatgccttat tatgtggaat gagaatttcc tgtgtaatct cttcaagacc
     3721 taatccgcct attcgtgatg gtgttccttt aaaatacctg tcaaccacat ccctgctaat
     3781 tccaattgct tcaaaaatct gagatgagcg gtagctcctc aaagtactta ttcccatctt
     3841 cgacattatc ttcagaaggc ctttgttgat tgcattaatg taattttctt ccgctttttt
     3901 gtagtcaagc tgaattactt tatctttaac aagtctctca attattgcaa aactcatata
     3961 tggatttatc acgcttgcac catatccgaa aagaagtgca aaatgcataa cttccctggg
     4021 ttctgccgat tctacaatta tgtcgatttg catccttttg cgtttctcaa ccagatagtg
     4081 atgtacagcc gaaactgcaa gcagcgatgg tataggtgca tgctcccggt ctatccctct
     4141 gtctgacagg attatataat tctttccctc atcaactgcc ttttcgcaat cgtaacaaag
     4201 tttttcaata gcctctttta gtccttcggc tccctttgaa acttcaaaat gcattttaaa
     4261 tactgagcat gagaaccctt tatatctgag gtttttaatt atgttgaaat aagtatttga
     4321 tataaccggt attttgaact taaccatttt cacatgatca ggagtggtgt caagaagatt
     4381 tttctgaaga gacccgaggt atcctgacag tgtcattaca agctcctctc tgataggatc
     4441 aataggtgga tttgtaacct gagcaaacag ttgtttgaaa taatcgaata accggtatgg
     4501 tttttcggag agcactgcca gaggagtgtc gtggcccatt gaagcaatag gctctttgcc
     4561 tgttgctgcc atttcagtaa ttatcttttc tatatcttct aatgagtaat taaatgacac
     4621 cagatatttt tcataatccc catctaaagc tggagaaaaa tgctgtccgg tttcgatatc
     4681 ttcaagaaga acctgatttg cttcaatcca ttttccataa gggtattctg atgccagttt
     4741 tgtctttatt tcatcatcat aatatatttt tccttcttca gtgtcgatga gaagtatttt
     4801 accagctctt agtcttcctt ttgcaacgac ttcatcaggt tcaaattcct gaacacctgt
     4861 ttctgaagcc attataataa ggtcattcga agttattgta aaccttgatg ggcgcagtcc
     4921 attccgatcg agcatccctc cgatatacct tccgtcagaa aaaatcaagg aagctggacc
     4981 atcccatggt tccataaatg ttgcatgata atgataaaaa tctctcagtt ctggaggtat
     5041 tggattttta atgttaattg attcaggaat cagaatagac attgcatatg gtagagattt
     5101 gccactcata accagaaact ccacaacatt gtcgagcgaa gctgagtcgc tctttccagg
     5161 ctcaataatc gggtaaattc tcgacaggtc gccaagcgct tctgatttaa gaatagattc
     5221 acgtgcttcc atccatattc tgtttcccct tattgtattt atctctccat tatggccaat
     5281 aaatctgaaa ggctgtgcaa gatcccagct tgggaatgtg ttggtactga atcttgaatg
     5341 tacaagggct attgcactct gaagccgttc atctttcagg tcaagataat attccttcaa
     5401 ttggtatgac gtcaacattc ccttatatac cagaacacgg gtcgaaaggc tcggaatgta
     5461 aaagaaatct ttctgaccaa ggtttgattc cctgattctt ttttcagtta tttttctgat
     5521 aatatatagt ttcctttcaa gaacttcagg ttcagtatct gcagtcatga aaatttgttt
     5581 catcaccggt tcagtcatca atgcaagttc tcccaataca ctgttatttc tcggcacttc
     5641 acgaaacccc agagttttaa caccttcttc tgccgccact tctcctaata tctttaaaca
     5701 ttcatctgct tcatggctgt tttgtggcag gaaaagtatt ccggttccaa aatatcccga
     5761 aggaggtaaa gcatacccct gaataaggta aaagtcacga ggaatctgga tcattattcc
     5821 tgcaccatca cccgtacggc tatctgcgcc ctcagcacca cgatgagcca tgttacataa
     5881 gatctcaagt ccttttgaaa taatggaatg tgattttcgc cctttgatgt gagctacaaa
     5941 cccaattcca caactgtcat gttcgaaatc acttctgtat aatcctctgt tggtactttt
     6001 catggttaac ctcattttta ttaaaaaaaa accgttctca ctggctgaga acggtttata
     6061 ataaatttaa aacaccattc tcagccttcg ctgaacctcc tgagaacgat tactgataat
     6121 attaaaaggc ctttcgcctc catttcctca aaattttcgt acaaatataa tatttgctta
     6181 aaatatttaa gaaaaattaa aaagaaattt attaacatta attatttaaa atttaattaa
     6241 gcatttttag tttccataac catcaggcaa aatataattt aacttaagga gtagtttttt
     6301 catttatagt tttaatttta tattagagaa tcaataataa ttatgcgttg aatgagtaat
     6361 caaagtgaaa ctctttttat tataattttc ggagccacgg gcgaccttac ctaccgaaag
     6421 cttgctcctg cactgtattc gcttaatatc cagggctcaa tacctgaaag gttttatatc
     6481 gtgggtgttg gaagaaaaaa tttaacagat gaagagttca gagcaagaat gcgtgaaggt
     6541 ataaaattgt tttccgataa aagtgaaata agagacagct ctatagaaga gttacttgag
     6601 catttcttgt atatcaaatc agacatggtt tctcatgaag gatacacgga tcttaaatca
     6661 ttgatcaaga agaaagaaga cgaatcaggt ggtgcaataa actgtatata ttatcttgcc
     6721 acacctcccg acctgttctc tgtgattcct ttcaggttac atgaagcagg gcttacttcg
     6781 gaagacaaag gatacataag gttcgttttt gagaagccat tcggatatga gtatgcttcc
     6841 tgcagaaagt tgaacgaaga ccttcaccgg attctttctg agaaacagat ataccggatt
     6901 gatcactacc tgggcaagga aaccgtccag aatattcttg ttatgagatt ttcaaacggt
     6961 ttatttgagc ctttgtggaa ccgcaattat attcaccaca ttcagattac ctctgccgaa
     7021 agcataggtg ttgaaaacag aggcggatat tacgactcgg ccggagcact gcgtgatatg
     7081 gttcagaacc acctgcttca gatggtgggg ttgactgcaa tggaaccgcc atcttctatt
     7141 catcccgatg ctataagaaa tgaagtgctg aaagtatttc aatcattgca gccattaaag
     7201 gaggaagagg taccagaata tgtaatccgc gggcagtata cagcttcgaa ggtaatggga
     7261 caatgtatac ctgcttacag acaggagccc ggggtaaatc cggcttctcg caccgaaact
     7321 tatgtggcaa tgaaattttt tatcaacaac tggcggtggg gtggtgttcc tttctttatc
     7381 aggaccggca aacgcctgcc caccagagta actgagatag ttgtcaattt taaacctaca
     7441 ccacattttc tgtttcatgt taatgatatg aataaggcca taaatcagct tattataaga
     7501 atacaacctg atgaaggtat tgtgcttaaa tttggaataa aaaagccagg cagcggtttc
     7561 aatgtcgaga atgttagtat ggatttccat tattcagatc ttttaaaggt tagagtccca
     7621 gaggcatacg aacgattgct cggagatgtg ataatgggcg attcaacgtt gtttgcgcgc
     7681 gatgatattg tcgaaacagc ctggaaattt cttgaacctg ttcagaaagc atgggctgaa
     7741 aaccgggaca taaaaatata tggttaccct gcaggtacat ggggacctga agaggcagga
     7801 aaaattattg gcgatggact tgagtggcgt tatccctgca aaaatctggc cgatgacgga
     7861 actttttgcg aactttaaca gtatggacag aataattaag atatcgaaca acataatggc
     7921 actctctcat gaagttgctg cagacctttc agctattatc aataacgcag tccggcgttc
     7981 ccaccacctg aacattgcac tttcaggtgg caatacgccg cgaacctttt attcagttct
     8041 gggtacacat tatgccctta gtacagaatg gaaagcagtt gatttcttct ggagcgatga
     8101 acgttgcgtc cctcccggtg acagggaaag taattacagg gttgcaagag aacacttttt
     8161 cgaacggatt aagatacagg ctggaaaaat tcacagaatc agaggtgaag atgatcctca
     8221 aacagaagca ataagatatt ctgaactaat caagaaaaaa gttccatttc gttttggatt
     8281 tcctgtattt gatattgttt tcctggggct tggaacggac ggacatatag catcaatatt
     8341 tccaggtgat gagaaagtac ttagttcggt cagaacatgt gaagttgcag tacatccaga
     8401 aacaggacaa aaaagaatta cacttaccct gccagtgata aataatgcag aaagaattat
     8461 ttttctcgtt tcaggaaaag ataaggctga aattgttgct gaaatactta ataacccgga
     8521 tgccaaacat tatcctgctg gcatggtaaa tcctcacttc ggcaaaatat actggtatat
     8581 ggatctggat gcagcatccc tgttaagcca gtatgcttaa aaaaataatg ttattgttaa
     8641 caattggttg aaaataatct tcagttaata gatttaatct ttataaattt atcctgcctt
     8701 ttaaactaaa taattaagat tatgtcgcac gagaaaccta aaggtgaagc caagaagaa
//
LOCUS       DAOSNB010000075         8721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8043_length_8721_cov_5.414378, whole genome shotgun sequence.
ACCESSION   DAOSNB010000075 DAOSNB010000000
VERSION     DAOSNB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8043_length_8721_cov_5.414378"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="mnmG"
                     /locus_tag="PLG42_04535"
     CDS             <1..1285
                     /gene="mnmG"
                     /locus_tag="PLG42_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOU30513.1"
                     /translation="GTSARIDGRSLDFSKMIEQKGDGDGRCFSFLHNKVFIKNEKSCY
                     ITYTNEEVHEELRKGLKFSPLYTGIIKGRGPRYCPSIEDKIVTFKEKKAHQMFIEPEG
                     WETNEYYINGFASSLPLDVQLKALRKIPGLENVEIFRPGYAIEYDYFPPTQLKHNLET
                     KLVDGLFFAGQVNGTTGYEEAAAQGLIAGINAVLKIKGKKPFVMTRDESYIGVLIDDL
                     VIKGIDEPYRMFTSRAEYRILLRQDNADERLTRKGYELGTIGEDRIRRLEEKEEMVNE
                     IIEFVKKESIEPEKINEFLTQKETSCLIQKTKLINIALRPQIGLKELLERVRKAKDLK
                     ALKSERVDEIFECAEIKIKYEGYIKRERQIADKIKRLETLRIPEDINYNELLSISTEG
                     RQKLNRIRPETIGQASRISGVSPSDINVLLMYLGR"
     gene            1330..2946
                     /gene="ade"
                     /locus_tag="PLG42_04540"
     CDS             1330..2946
                     /gene="ade"
                     /locus_tag="PLG42_04540"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOU30514.1"
                     /translation="MISGNLIDIFERTIYPAEIEIENGRIRKIKRKERNEEFYIIPGL
                     IDSHIHIESSMCTPGSFSEEAVSRGTTAVVSDPHEIANVLGIAGVNFMIEDGLKVPLK
                     FFFGAPSCVPATPFETSGAEIGINDIDMLLSKPEIKYLAEMMNFPGVIYEDKDVLSKI
                     KIAHKYKKPVDGHAPGLSGDNLRKYIEAGISTDHECVTLEEAKEKIGLGMKVMIREGS
                     AARNLDSLKELIRTNPDMVMLCSDDLHPEMLARRHIGLLVAQLVNEGFDLFDVIRLCT
                     LNPTRHYKLETGLLREGDPADFIIVDTPYRMNVLETWINGEKVFDRGKLLYEYKGGTP
                     ANKFNCSEITTQQISVKISGNQIRVIEAFDGQIFTKEVIVPVRNDSFITQNPSEDLLK
                     IVVKDRYNDSSLAVGFIKGFGLKKGAFASSVAHDSHNIICVGTNDNDIVNAINAIIKL
                     RGGLSVAAGSNIETLSLPIAGIMSDRPVKEIAGKYEILSGIVKELGCQLEAPFMTLSF
                     MALLVIPELKLSDRGLFDSKQFKFVPLTVS"
     gene            3058..4863
                     /gene="uvrC"
                     /locus_tag="PLG42_04545"
     CDS             3058..4863
                     /gene="uvrC"
                     /locus_tag="PLG42_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOU30515.1"
                     /translation="MTIDPKSILPVLPSKPGVYQFLDSSGRILYVGKARNLKKRISSY
                     FSKRQPGKTAVMLSHAASLHHIVTETESDALLLENNLIKKHQPRYNILLKDDKTYPWI
                     CVKKEPFPRIFSTRKVIRDGSEYFGPYTSVQMVKTILDLVRQLYPLRTCNLSLTENNI
                     KKGKFKACLEYHIGRCKAPCIGLQTAEDYSFSISQIRDIIKGNISEVINHLEALMKSY
                     ASELRFEDAQRVKEKIELLSRYRSKSVIVSTFIRNVDVFGYAEDNNRAYINYLRVHNG
                     AVIQAFTLEIQSRLEEEKETLLGLGITEIKQKISSNAREAIVPFLPDFPEPGIKYTVP
                     AAGEKRKLLELAVRNAMYYRQEQQKRREEREREKEKKSKLEKVKADLHLPLIPKHIEC
                     FDNSNLMGENPVAACVVFRNGIPSKSEYRHYNIKTVKGPDDYSSMEEIVYRRYSRMLD
                     ENQSLPDLVIIDGGKGQLNAALKSLEKLNLETKIPVIGIAKKLEEIYFPGDSLPFYLD
                     KTSYTLRLIQQIRNEAHRFGLSFHRKKRSMQMIFSELDNIKGIGPKTKEILMKHFNSI
                     DDIKSAPGQEIEKLIGKSKARILRNHFSANTRNET"
     gene            complement(4961..5578)
                     /locus_tag="PLG42_04550"
     CDS             complement(4961..5578)
                     /locus_tag="PLG42_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOU30516.1"
                     /translation="MIKAIIFDLGNVLINWKPADYLKNAGYKDTEISLILKDVFHSEE
                     WLKLDNGDITTPQAIKIIAKKSTLSESEIRAIFDLRLKIISPIHNNAKLLPELKKRGY
                     RLYYLSNFPDDIFDEVKNKYNFFMFFDGGEISARLNASKPDKRIFRIFLDKYMLKPEE
                     SLFIDDSDINAGAAEKLGIKAIHLDSPEVLKEKLEEALGIKLKIS"
     gene            complement(5575..6900)
                     /locus_tag="PLG42_04555"
     CDS             complement(5575..6900)
                     /locus_tag="PLG42_04555"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU30517.1"
                     /translation="MTHEDFIVKTLGRTNIKSPLLQTVRNYTPLFKFLEEDERILYDV
                     SLSNFKRCLEKGENPLSFEKAGPRENIFFEPAKTKVAIVTCGGLCPGLNNVIRSLVHE
                     LYYRYGVTRILGIKYGYEGLVAKYNHPVVELTPELVSNIHLSGGSFLGSSRGHQDPAQ
                     MVDTLEILNVNILFCIGGDGTLRGAHAIHEEITKRNLKIAVAGIPKTIDNDISFIEKS
                     FGFETAFSIANDIIRNAHNEASGAYNGIALIKLMGRDSGFIAAHAALSIQEVNFVLIP
                     EVPFDLYGPRGFLKILRKRLEEKHHAVIVVAEGAGQDLFEKTEEIKDASGNIKHRDIG
                     VYLKEKITEEFTDKGFPFSLRYIDPSYIIRSAPAIPNDSKFCNLLAQNAVHAAMAGRT
                     DFVVGYWNDMFTLIPIPLATSQRKKIDVNGELWWHVLEATGQPSTMKNP"
     gene            complement(6959..7864)
                     /locus_tag="PLG42_04560"
     CDS             complement(6959..7864)
                     /locus_tag="PLG42_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOU30518.1"
                     /translation="MKTYRLLLLLICVFNFSHRAEGQNKNKNIIIAPGAEVIKLAGDF
                     SFTEGPAADRKGNVYFTDQPNNRIMVWTIEGKLETFMQPAGRANGLFFDKDGNLWACA
                     DEKNELWKISPDKKIEIIVQKYNNAVFNGPNDLWIAPGGGIYFTDPFYRRTWWDHTER
                     PQDKQCVYFLPPGRKEPIRIIDDLVQPNGIIGTPDGKILYVADIGDKKTWKYIINSDG
                     TVSGKTLFCEMGSDGMTIDNKGNLYLTGNGVTVFDRSGNRILNIPVPEKWTANVCFGG
                     KDRKTLFITASTGLYAIKTKVKGVY"
     gene            complement(7875..>8721)
                     /locus_tag="PLG42_04565"
     CDS             complement(7875..>8721)
                     /locus_tag="PLG42_04565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU30519.1"
                     /translation="VLLNKDLSFAAGLNQFSGADPFKIITLGKTGIRTTLLGMGTGYS
                     GYNRSSNITRAGVAESVIKAAYEKGIRYFDCADSYGTHPFTREALKSYPRESYVIGTK
                     MWVLRGGIPEPERPDAEIVVDRFRKELNTDYIDIVQLHCMTSGTWTDEQKRFMDGLEN
                     LKAKKIIRAHGVSVHSLEALKAAAESPWVDVIHVRINPFGESMDSKDPDEVLPVIKKI
                     HDSGKGVIGMKLIGNGKFRNDPEKINASLKFVLGLGTVDMIIIGFEKPEQIDDYINRM
                     KNKVL"
BASE COUNT         2843 a         1549 c         1686 g         2643 t
ORIGIN      
        1 aggaacaagt gcaaggattg atggtaggag tttggatttt tcgaagatga ttgagcaaaa
       61 gggcgatggt gatggaagat gtttttcttt tctgcataac aaggtattta ttaaaaatga
      121 aaaaagttgt tatataactt atactaatga agaagtacat gaagagttaa gaaagggatt
      181 gaaatttagt cctttgtata caggaataat aaaaggaaga ggaccaagat attgtccaag
      241 catagaagat aaaatcgtaa cgtttaaaga aaagaaagcg catcaaatgt ttatagagcc
      301 tgaaggatgg gaaacaaatg aatattatat aaatggattt gctagtagct tgccgcttga
      361 tgttcaattg aaagcgttaa gaaaaatacc aggtcttgaa aatgttgaaa ttttcaggcc
      421 tggatacgca atagaatatg attattttcc accaacgcaa ttaaaacata accttgaaac
      481 gaaattggtt gatggattat tttttgcagg acaggtaaat ggtaccacag gctatgaaga
      541 agccgcagcg caaggattaa tagcagggat aaatgctgtt ttaaaaatta aaggaaagaa
      601 accatttgtg atgacaagag atgaatcata tataggagtt ttaattgatg atctggtcat
      661 aaaagggatt gatgaacctt ataggatgtt tacttcaaga gctgaataca gaatacttct
      721 tagacaggat aacgctgacg aaagattaac acgaaaagga tatgaactgg gaacaattgg
      781 agaagataga ataagaagat tagaggagaa agaagaaatg gtaaatgaaa ttatcgagtt
      841 tgtaaaaaaa gaaagcattg aaccagaaaa gataaatgaa tttttaacgc aaaaggaaac
      901 ctcatgctta atacaaaaaa caaaattgat aaatatagca ctaagacctc aaatcggttt
      961 aaaggaatta ctggaaaggg taaggaaagc aaaagatcta aaggctttaa aatcggaaag
     1021 agttgatgaa atatttgaat gtgccgaaat taagataaaa tatgaaggat atattaaaag
     1081 agagagacaa attgctgata aaataaagag gctggaaaca ttaagaattc cagaagacat
     1141 aaattataat gaattattat caatatcaac agaaggaaga caaaagttga accggataag
     1201 accagagacc attggtcagg caagcagaat tagtggtgtt tcaccttcag atatcaatgt
     1261 tttgcttatg tatctgggga gataaatgtt ccacgtggaa cattttttta aattttaaaa
     1321 tttattttca tgatatctgg caatttgata gatattttcg aaagaacaat ataccctgct
     1381 gagattgaga tagagaacgg caggataaga aagataaaga gaaaagaaag aaatgaggaa
     1441 ttttacataa tacccggact aattgattcc catatccata tagagagttc aatgtgtaca
     1501 ccggggtcat tttctgaaga agctgtttca cgtggaacaa cagcagtagt ttctgaccct
     1561 catgaaatag caaatgttct gggtattgct ggagttaatt ttatgattga agatggactg
     1621 aaggttccct tgaagttctt cttcggagca ccttcgtgtg taccagccac accttttgaa
     1681 acttcaggag ctgaaatcgg aataaacgat attgatatgc tcctgagcaa accagagata
     1741 aaatatctgg ccgaaatgat gaatttccca ggtgttattt atgaagataa agatgtatta
     1801 agcaaaataa aaattgccca taaatataaa aagccagttg acggacatgc acccggatta
     1861 tcaggagata acctgagaaa atatattgag gctggtattt ctaccgatca tgaatgtgtg
     1921 acccttgagg aagctaaaga aaaaataggc cttggaatga aggtcatgat tcgtgaagga
     1981 agcgctgcaa gaaatctgga ttctttaaag gaactaataa gaacaaatcc ggatatggtc
     2041 atgttatgta gtgacgacct gcatccggag atgcttgcaa gaaggcacat tggactactt
     2101 gttgcacaac ttgtaaatga aggttttgac ctctttgatg ttatacgact gtgcacctta
     2161 aaccctaccc ggcactacaa acttgaaacc ggacttctca gagaaggcga cccggcagat
     2221 tttattattg ttgatactcc ttacagaatg aatgttctgg agacctggat aaacggggag
     2281 aaagtcttcg atcgtggtaa actactttat gaatataaag gaggaactcc tgcaaacaaa
     2341 ttcaattgtt ctgaaataac aacccaacaa atttcagtaa agatcagcgg aaaccagatc
     2401 cgtgtaatag aagcatttga tggacaaatt tttacaaaag aagtaattgt gccggtaaga
     2461 aatgattcat ttataactca aaatccttct gaagatctcc tgaaaattgt tgttaaggac
     2521 agatataatg actcttccct tgcggtaggt tttataaaag gcttcgggct caaaaagggt
     2581 gcgtttgcaa gctctgtagc acacgacagc cataacatca tttgtgttgg tacaaatgat
     2641 aatgatatag taaatgcaat aaacgcaatt attaaactac ggggaggact atctgttgca
     2701 gcgggtagta atatagaaac gctttcgcta cccattgctg gaataatgtc tgacagaccg
     2761 gtaaaagaga tagctggaaa atacgaaata ttgtcgggaa tcgtaaaaga actcggctgc
     2821 cagcttgaag caccctttat gacactatct ttcatggcac tgcttgtaat acctgaactt
     2881 aagttaagtg atcgtggcct gtttgattca aaacagttca aatttgttcc cctcacagtt
     2941 agctgatatt ttctgatatt ttaaagatta atgcattgta attcaacata ataacaatta
     3001 gatgctgaat tacaggaaag taattatttt agtatttttg catgcaactg gttaataatg
     3061 acaatagatc cgaaatcaat actgccagtg cttccttcaa agccaggagt atatcagttt
     3121 ctggattcct cgggcaggat attatatgta ggaaaagcac gtaatctcaa aaagagaata
     3181 agctcatatt tttcaaaaag acagccggga aaaacagctg taatgctgag ccacgcagct
     3241 tcgcttcacc atattgttac agaaactgaa tcagatgccc tgctgctcga aaataatctg
     3301 attaagaaac atcaacccag atataacatt cttcttaaag atgataaaac atatccatgg
     3361 atttgtgtta agaaagaacc gtttccaaga attttcagta cccgcaaagt tatacgcgac
     3421 gggtcggaat atttcggtcc ttatacttca gttcaaatgg tcaaaacaat actggacctt
     3481 gtaagacaac tttatccttt gaggacctgt aatctgagtc ttactgaaaa taatataaaa
     3541 aaaggaaaat tcaaagcatg tctcgaatat catattggaa gatgtaaagc cccatgtatt
     3601 ggattgcaga cggcagaaga ttatagtttc agcatttcac agatccggga cattattaaa
     3661 ggcaatattt ccgaggtgat aaatcatctc gaagctctta tgaaatctta tgcctctgag
     3721 cttagatttg aagatgctca gagggtaaaa gaaaaaatag aactgctttc aagatacaga
     3781 agtaaatcag tgattgtaag tacgtttatc agaaatgttg atgttttcgg atatgctgaa
     3841 gataataaca gggcttatat taattatttg agagtacata atggcgctgt aatacaggct
     3901 tttacactag aaattcaaag tcgtctggag gaggaaaagg agactcttct cgggcttggt
     3961 attactgaaa taaaacagaa gatatcaagt aatgccagag aagcaatagt gcctttttta
     4021 cctgattttc ctgagccagg tataaaatat acagtgcctg cggccggtga aaaacgtaaa
     4081 ctgcttgaac ttgctgtcag aaatgctatg tattacaggc aggagcaaca aaagcgaagg
     4141 gaggaaaggg agagggaaaa agaaaaaaaa tcaaaacttg aaaaggttaa agcagactta
     4201 cacctgcctt taattcccaa acatattgaa tgttttgata attccaatct catgggagaa
     4261 aacccggtcg cagcatgcgt tgttttcaga aacggaatcc cttcaaaatc agaataccgg
     4321 cattataata taaaaacagt gaaaggtccc gatgattact cttcaatgga agagatcgtt
     4381 tatcggcggt atagcagaat gcttgatgag aatcagtctt tgcccgacct ggtaataatt
     4441 gacggtggaa aaggacagtt gaatgccgca ttgaaaagcc ttgaaaaact taatctcgag
     4501 accaaaatac cagttatagg cattgctaaa aagctggaag agatatattt tccaggtgat
     4561 tcacttcctt tttaccttga taaaacttca tataccctgc gccttattca gcaaataaga
     4621 aatgaagccc accggttcgg attaagtttt caccggaaaa aaagatccat gcaaatgata
     4681 ttttcagaac tggacaatat aaaaggaata ggccctaaaa caaaagagat tctaatgaaa
     4741 cattttaatt caattgatga tattaaaagt gcccctggac aggaaattga aaaacttata
     4801 ggcaaatcaa aagccagaat attacgaaac cacttttcag ctaatacccg gaatgaaaca
     4861 taattcgact ttcgaccaga tagataccaa tataccgatt aagtatatgg aaatattata
     4921 agattaaatg aatggtcaat tatcagtttt gcactataaa tcaactaatc ttcaatttta
     4981 tgccaagagc ttcctcaagc ttttctttaa gaacttctgg actgtcaaga tgaattgctt
     5041 taatgccaag tttttcagct gcccctgcat taatatctga atcatcaata aacagagact
     5101 cttccggttt gagcatatat ttatcaagaa atattctgaa aattcttttg tcgggctttg
     5161 aagcgttaag ccttgccgaa atttctccac cgtcaaaaaa catgaaaaag ttgtatttgt
     5221 ttttcacctc atcgaaaata tcatcaggga agtttgacag ataatagagt ctgtagcctc
     5281 tttttttaag ctccggaagg agtttggcat tattatggat gggagatatt attttcagcc
     5341 ttaaatcaaa tatggccctt atttcgcttt cagaaagggt agattttttt gctattattt
     5401 ttattgcctg tggagtggtt atatctccat tatccagctt aagccactcc tcactgtgaa
     5461 aaacatcctt aagtatgagg ctaatttctg tatctttata acctgcattt ttcaaataat
     5521 cagctggttt ccagtttatc agaacattac ctagatcaaa tattattgct tttatcatgg
     5581 gttcttcata gttgaaggct gacctgttgc ttcgagcaca tgccaccaca gttcaccgtt
     5641 gacgtctatt ttcttccgct gtgaagtggc aagcggaatg ggaataagcg taaacatatc
     5701 attccagtag ccgacaacaa agtctgttct acctgccatt gccgcatgta cggcattttg
     5761 tgccagaagg ttgcagaatt tgctgtcatt cgggatggca ggcgcgctcc ttattatata
     5821 acttggatca atatatctca ggctaaaagg aaagccttta tcggtaaatt cttcagttat
     5881 cttttctttc agataaacac ctatatccct gtgtttaata tttcctgaag catctttgat
     5941 ttcttcggtt ttttcaaaaa gatcctggcc ggctccttct gcaacaacaa tcactgcatg
     6001 atgtttttct tcgagtcttt ttcttaaaat cttgagaaaa cctctgggac cataaagatc
     6061 aaatggtact tcgggtataa gcacaaaatt cacttcctga attgataagg cagcgtgagc
     6121 agctatgaaa cccgaatcgc ggcccattag cttaatgagt gcgatgccgt tatatgctcc
     6181 tgatgcttca ttatgagcat ttctgattat gtcatttgca attgaaaagg ctgtttcaaa
     6241 tccaaaggat ttttcaataa aacttatgtc gttatcaatt gtttttggta ttcctgcaac
     6301 tgcaatttta aggtttcttt ttgtgatttc ttcgtgaatt gcatgagctc ctctcaatgt
     6361 cccatcaccg ccaatgcaaa atagaatatt aacgtttaaa atttcgaggg tatcaaccat
     6421 ttgagccgga tcctgatgcc ctcttgatga gcccagaaag ctaccgccac tgagatgtat
     6481 attgcttacc aattcaggag tcagctcaac aactggatga ttgtatttag ctaccagacc
     6541 ttcataacca tactttatgc cgagaatcct ggtaactcca tatctgtaat agagctcatg
     6601 tacaagactt ctaattacat tatttagtcc gggacataat ccgccacagg ttactatagc
     6661 aacttttgtt tttgcaggtt cgaaaaatat attttcgcgt gggcctgctt tttcaaaaga
     6721 aagcgggttt tcaccttttt caaggcagcg tttgaaatta gaaagagaaa cgtcatagag
     6781 aattctttcg tcctcttcca gaaatttgaa tagaggagta taatttctta cggtctgaag
     6841 taatggcgac tttatatttg ttcgcccaag ggtcttaaca ataaaatcct cgtgggtcat
     6901 tattttactt tttatggatt tacagctgat gcttttacat tatctgataa cctgtatctt
     6961 aataaactcc ctttacttta gtttttatag cgtaaagacc tgtgcttgca gttatgaata
     7021 gagttttcct gtctttgcct ccgaagcata catttgctgt ccatttttca ggtacaggta
     7081 tgttgagaat tctgtttcct gacctgtcaa atactgttac accgtttcct gtaaggtaaa
     7141 gattcccttt gttatctatt gtcatgccat ctgaacccat ttcacagaaa agggttttgc
     7201 ccgaaacggt cccatcagag ttgataatat atttccatgt ctttttgtcg cctatgtcgg
     7261 ctacataaag aattttaccg tcaggagttc cgataatccc attaggctga accagatcat
     7321 caataattct tatcggttct tttctgccgg gaggcagaaa atatacacat tgtttgtcct
     7381 gagggcgctc cgtatgatcc caccatgttc ttctgtaaaa agggtctgtg aaatatattc
     7441 ctccgccggg tgcaatccag agatcatttg gtccattgaa tactgcatta ttatactttt
     7501 ggacaataat ttcaattttt ttgtcaggag atattttcca gagttcgttt ttctcatcag
     7561 cacatgccca tagattaccg tctttatcga aaaacagacc attggctctt ccggcaggtt
     7621 gcataaatgt ttcaagtttt ccttcgatag tccataccat tattcggtta ttaggctgat
     7681 cggtaaagta aacgttacct tttctatctg ctgcaggacc ttcagtaaaa gaaaaatctc
     7741 ctgccagttt tataacttca gcgcctggag caataataat attcttgttt ttattttgac
     7801 cttcagcccg atgcgaaaag ttaaaaacgc atattaataa taacaatagc ctgtatgttt
     7861 tcatggctgt aattttacaa taccttattt ttcattctgt ttatataatc atcaatctgt
     7921 tcaggttttt caaagcctat aattatcata tccactgtac ccagtccgag aacaaattta
     7981 agtgaagcat taatcttttc cggatcattt ctgaattttc cgttaccgat aagtttcatt
     8041 ccaataactc cctttcctga atcatgaatc ttttttatta caggtaaaac ttcatcagga
     8101 tctttactgt ccatactttc accaaaagga tttattctaa catggataac atcaacccac
     8161 gggctttctg cagctgcttt aagtgcttcg agtgaatgta ccgaaacacc atgggctctg
     8221 attattttct tagccttaag attttcaagg ccatccataa atcttttctg ctcgtcggtc
     8281 catgttccgg aagtcatgca atgaagttga acgatgtcga tatagtcagt atttagttct
     8341 tttctgaatc tgtctacaac tatttctgca tctggtcttt ccggttccgg gattcctccc
     8401 ctaagtaccc acatttttgt tccaattacg taagattcac gaggatacga tttgagtgcc
     8461 tcccttgtaa atgggtgggt accatagctg tcggcacaat caaagtaccg tatacccttt
     8521 tcatatgctg cctttattac agattcagca actccggctc ttgtaatatt tgaagagcgg
     8581 ttgtaaccac tgtagcctgt tcccatgccg agaagtgttg tccggatacc tgtttttcca
     8641 agtgttatta ttttaaacgg gtcagcacca gaaaactggt taaggccagc ggcaaaagat
     8701 aagtctttat tcaaaagaac g
//
LOCUS       DAOSNB010000076         8692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8073_length_8692_cov_4.564316, whole genome shotgun sequence.
ACCESSION   DAOSNB010000076 DAOSNB010000000
VERSION     DAOSNB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8073_length_8692_cov_4.564316"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLG42_04570"
     CDS             <1..670
                     /locus_tag="PLG42_04570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU30520.1"
                     /translation="TETINGTSRVRWGRIIISGLIWMIISAIYLFIQIEINRENFVLN
                     NLSNTLIMLVIISVIFIPFQAAFEEILFRGYLMQGFAVLTRNRWAPVLLTSLFFALMH
                     IANPEIKEFGVLTMMPQYFLFGLIFGITTVIDDGIEIALGAHAANNIFLSVMLTHKSS
                     TLQTPAIFEQTTIHPWNEFVGLLISSIIFLAILKYIFRWKNLKIMLSKLDRTEKIQMQ
                     EFLQ"
     gene            complement(720..1733)
                     /gene="dusB"
                     /locus_tag="PLG42_04575"
     CDS             complement(720..1733)
                     /gene="dusB"
                     /locus_tag="PLG42_04575"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOU30521.1"
                     /translation="MKIGNINLGDRPLFLAPMEDVTDTSFRLLCKSFGADFMYTEFVS
                     SDGLIRNGSKSVKKLILLDEERPIGIQLYGHIVDVMVQAALMAEQSNPDLIDLNFGCP
                     VKKIANRGAGAGMLRNVPLMVEMTSAIVKAVKLPVTAKTRLGWDENNKNIMEVAEKLQ
                     DAGISAITIHGRTRSQLYKGEADWTLIGEVKNNPRIKIPVIGNGDINGPLKAKEMFDK
                     YGVDGIMIGRASVGRPWIFREIRHYLDTGELLPEPTVAEKADIALKHFDLSLKYKEGL
                     TAIYEMRRHLSNYFKGIPHFKETRLKLLTSTDADEIKSIILDIKHKWGDFRSNENTSI
                     YQL"
     gene            1793..2740
                     /locus_tag="PLG42_04580"
     CDS             1793..2740
                     /locus_tag="PLG42_04580"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOU30522.1"
                     /translation="MNLFSQVAQDRSPLNMLIILVLLILVAGTIVFSLFLLAGSVIFN
                     IGISCFMDLSENLPVSDAGVIRFIQVAQQLSFFLLPAILFLLIYFKKDSGLDLFTGNV
                     KTTEILMVFILAFMATYVTAWTGNINSKLVLPQALSGIEEWMKEKESKAAEITNFLIK
                     TPDAYTYILNLIIMAIIPAVSEELIFRGVIQQVFRKLIRSGHLAVWITALIFSAIHLQ
                     FYGFLPRLILGLIFGYIFIWTGNILMPIAAHFFNNAIAITFSFIFGWDRITDVFSEKG
                     VMKMTEVPFIQILTGGIVLFYFWMNHRKMSASGTNTPEA"
     gene            complement(2743..3462)
                     /locus_tag="PLG42_04585"
     CDS             complement(2743..3462)
                     /locus_tag="PLG42_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30523.1"
                     /translation="MPYRRLPNTDSARIRALRTALLIGNETPPHKLAFSQKSLMKLQK
                     FLPEFENAIKLQRQYFTSQARKVKNYWEIVRKARIYISHFIRVMNMAIYRGELPSEIR
                     AYYGLPTNELNVPSLNTENEIVSWGKRIIDGETLRIKKGGTPITNPTIAVVKVRYEQF
                     IDALNAKKTLNEKASLMSEKNNELRKEADELIQNIWNEVEKYYSTLPAGIRREKCEEY
                     GIIYYLRKGELAEQNKTAEKV"
     gene            3625..5010
                     /locus_tag="PLG42_04590"
     CDS             3625..5010
                     /locus_tag="PLG42_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOU30524.1"
                     /translation="MFSSEVYSDRRQKLRSLVKSGLAFFPGNVESPVNYPANPYHFRQ
                     DSSFLYFFGLDLPGLAGVIDFDSGDEWIFGDDFDLNDIVWMGPQPTVRELAMKCGIVK
                     TAPLKKLEEIIADAVSKKRKIHFLPPYRGETKMMLGALLKEDPCKMKTLASEELIRAV
                     VSLRIIKEAIEIEEIEKAVNISWEMHVTAMKMCRAGVKEQEIFGVIEGIAWSKGAGPA
                     FPVILSINGQTLHNHAHNNILTEGRMMVTDAGAESLLHYASDITRTTPVGGKFNSRQR
                     DIYEVVLRANNLAIGEAKPGKSFRQIHIDACKAIAQGMKELGLMKGDTEEAVMQGAHA
                     LFMPHGLGHMLGLDVHDMEGLGENYVGYNDRTKRSDQFGLSFLRFALECKPGHVITIE
                     PGCYFIPQLIDQWKSEGKFKEYICYDRLESYRDFGGIRIEDNIAITETGCRLLGRPIP
                     KTVEEIESVCS"
     gene            complement(5007..6404)
                     /locus_tag="PLG42_04595"
     CDS             complement(5007..6404)
                     /locus_tag="PLG42_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU30525.1"
                     /translation="MKKIFITFFLIMSLFHATSQTIVNRDPLIRKMVDAISGENLELY
                     VRKLVSFHTRHNMSEQNNPERGIGAAWNWIKAEMEKNIPQSGGRLSVRFEEYKAGGEG
                     QRIPQEILLKNVVATLKGINPDDDRVIVIGAHFDSRAEADNDFTSFAPGADDDASGIA
                     ALLEMLRIMSPVKFSATIVFIAFSGEEHGLYGSRFMAAKAKQENWNIIAMLNNDMIGN
                     SGSSETLLNDNMRVRVFSEGVPAFETEQMAALRRYTSGENDSRARQLARYIKEIGERY
                     VDQINVTLVFRNDRFGRGGDHTPFSQQGFPAVRICEFNENYDRTHKVPALVNGIQQGD
                     LPEYVDYEYVRKNTGINLATIANLALAPYEPVNCGIVTAGLSNTTTLRWEEPSKGIKP
                     AGYYVLIRETYQPLWDKKIFVTGTEVTLPYSKDNYFFAIQSVDASGHESIAVFPGRAV
                     RQTQSQPVRTNTGRN"
     gene            complement(6515..7261)
                     /locus_tag="PLG42_04600"
     CDS             complement(6515..7261)
                     /locus_tag="PLG42_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOU30526.1"
                     /translation="MIRIMKLTLKIWRQKDSKSKGRFETYHMENVSPEMSFLEMLDAL
                     NKRLVEEGGDPVAFDHDCREGICGSCGIYINGHPHGPVPGITTCHLSMRYFNDGETLW
                     VEPWRAKPFPVIKDLIVDRSAFDKIQIAGGFISVDTGSAPEANSIPVRKQNADMAFDA
                     AICIGCGACVAACKNASAMLFVSAKISQFALIPQGRIEAKERVRAMVEKMDELGFGNC
                     SNTGACEAECPKKIKISNIARLNREYYKAY"
     gene            complement(7432..>8692)
                     /gene="sdhA"
                     /locus_tag="PLG42_04605"
     CDS             complement(7432..>8692)
                     /gene="sdhA"
                     /locus_tag="PLG42_04605"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HOU30527.1"
                     /translation="HAVVLATGGYGTIYYLSTLAVNSNPSAAWKAYKRGALFANPSFI
                     QIHPTCVPQLNDYQSKLTLMSESLRNDGRMWVPKTKGDTRNPNEIPEEERDYFLERRY
                     PAYGNLVPRDVGSRAVKERCDAGYGVGKTGLAVYLDFRDAIKKQGKKAIEDKYGNLFE
                     LYKTITGKDPYEEPMMIYPAGHYTMGGLWVDYELMTTIPGLYAIGECNFSDHGANRLG
                     ASSLMQAAGDGYFILPNTISNYLADQIKTPRIPVNLPEFEESERSSIERLEKLLAVNG
                     RETASSFHKRLGKIMWDYVGMVRNEEGLTKALSQIRELREEFWKNLKVPGSINGLNQE
                     LEKAGRVADFLELAELLVIDALNRKESCGAHFREEYQTPDGEPLRNDKEYAYVAAWEY
                     TGHGNPPVLHKEELKFEVVEMKERSYK"
BASE COUNT         2324 a         1749 c         1777 g         2842 t
ORIGIN      
        1 tactgaaaca attaatggca cctcaagagt gagatgggga aggattataa tttctggttt
       61 aatctggatg ataatttcag caatatattt gtttatacaa atagagatca acagggaaaa
      121 ttttgttttg aacaatctta gcaataccct gataatgctg gtgattattt cggtaatttt
      181 cattcccttt caagctgctt ttgaagagat tcttttcagg ggatacctga tgcaaggttt
      241 tgcggtgtta acacgaaaca gatgggctcc tgtattgttg acatcactgt tttttgcgtt
      301 gatgcatata gctaacccgg agataaaaga attcggtgtt cttacaatga tgccacaata
      361 ttttttgttc ggactaattt ttggaataac tacggttatt gatgacggta ttgaaattgc
      421 actgggagcc catgctgcga acaatatttt tttgagtgtg atgttaacac ataaatcttc
      481 aacattacaa acgccagcta tttttgaaca aacaaccatt catccgtgga atgaatttgt
      541 agggctgctt atttcatcaa taatttttct ggctattctt aaatatattt tccgatggaa
      601 aaatttgaaa ataatgctca gtaaactaga tagaacggag aaaattcaaa tgcaggagtt
      661 tttgcaatga ttaagagtct ttaaaactga aacatttttc ttcggcagct ggctttctgt
      721 cagagttgat atattgatgt attttcgttg gatctgaaat ctccccattt atgtttgata
      781 tcgaggataa ttgattttat ttcatcagca tcggttgatg tgagtaattt caagcgggtt
      841 tctttgaagt ggggtattcc tttgaaataa ttcgaaagat gtcgtcgcat ttcgtaaatt
      901 gccgttaatc cttccttata tttcaatgac aggtcgaaat gtttcagggc tatgtcggct
      961 ttttcggcca cagttggttc aggcagaagt tcacctgtat cgagatagtg tcgtatttct
     1021 ctgaaaatcc agggccggcc cacagatgct ctgccaatca ttattccatc aacaccatat
     1081 ttgtcgaaca tttcctttgc tttcagagga ccatttatat cgccattacc tatcacaggt
     1141 attttaattc tgggattgtt tttcacttct cctataagag tccagtcggc ctctccttta
     1201 taaagctgag accgggttct gccatgaatg gtaattgcac ttattcctgc atcctgtagc
     1261 ttttcagcca cttccataat gtttttattg ttttcatccc agccaagccg tgttttggca
     1321 gtgactggca actttaccgc tttgactatt gcagatgtca tttcaaccat caggggcaca
     1381 ttacgcagca taccggctcc ggccccgcgg ttggcaattt tttttacagg gcaaccaaaa
     1441 ttcagatcta tcagatcagg atttgattgc tctgccataa gtgctgcctg taccattacg
     1501 tcaacgatat gaccgtaaag ttgaatacct atgggtctct cttcatcaag aagaatgagc
     1561 ttcttgacgc tcttgctgcc atttcttatc aatccgtcgg aagaaacaaa ttcagtatac
     1621 ataaagtctg ctccaaagct tttacacagc aacctgaaag atgtatctgt cacatcttcc
     1681 attggagcaa gaaacaatgg acgatccccc aggtttatgt tcccaatttt caaaatagct
     1741 gattttaaaa aagttatttc tttatttttg tgcaaattta attaaaatgc gcatgaatct
     1801 tttttctcag gttgctcaag acagatcgcc cctgaacatg ctgataattc tggtgttatt
     1861 aatcctggtt gccggcacca ttgttttcag tctgtttctt cttgcaggtt cagtaatatt
     1921 caatattggg ataagttgtt ttatggattt atcggaaaat ttaccggttt cagatgcagg
     1981 tgttatcaga tttatacagg ttgcccagca gctctcattt tttctgcttc ctgcgattct
     2041 gtttctttta atttatttta aaaaagattc aggtttagat cttttcaccg ggaatgtaaa
     2101 aacaacagag atattaatgg ttttcatact ggcttttatg gcaacgtatg ttacagcatg
     2161 gaccggcaac attaattcga aacttgtttt accacaagca ctttcaggaa ttgaggagtg
     2221 gatgaaagaa aaagaatcaa aagctgctga aataacgaat tttcttatta aaacacctga
     2281 tgcatatact tatattctta atcttattat tatggcaatt attccggcgg taagtgaaga
     2341 gttgatattc agaggggtta ttcagcaggt tttcagaaag ttgattcgtt cagggcatct
     2401 ggctgtatgg attacagcgt taatattcag cgctatacat ttacaatttt acggtttttt
     2461 accaagactt attctgggct tgattttcgg atatattttt atttggacag ggaatatcct
     2521 tatgcctata gctgctcatt ttttcaataa tgcaattgct attacttttt cttttatttt
     2581 cgggtgggat aggattactg atgtattttc cgaaaagggt gtcatgaaga tgacagaagt
     2641 gccttttatt cagatactta caggtggtat agtattgttt tatttctgga tgaaccacag
     2701 aaaaatgtca gcaagcggta cgaatactcc tgaagcatga ttttaaactt tttctgcagt
     2761 tttattttgc tcggcaagtt ctccttttct cagataatat ataataccgt attcctcgca
     2821 cttttctctt cttataccag ccggcagtgt ggaataatat ttttctactt cattccagat
     2881 attctgaatt aattcatcag cttcctttct gagttcattg tttttttcag acattagtga
     2941 agctttttca tttaatgttt tttttgcatt caaagcatcg atgaactgct cgtagcgaac
     3001 tttaaccaca gcaattgtgg gatttgtaat aggagttcct ccttttttta tccgcagggt
     3061 ttcaccatcg ataattcttt ttccccagct tacaatttcg ttttcggtat tgagcgacgg
     3121 cacgttcagt tcattggtag gaagtccgta ataggctctt atttcagagg ggagttctcc
     3181 tcggtaaatt gccatattca taacccttat aaaatgtgaa atatatatcc gggctttacg
     3241 gactatttcc cagtagtttt ttactttcct tgcctgtgag gtgaaatact ggcgttgtaa
     3301 ttttatagca ttttcaaatt cgggtaaaaa tttttggagt ttcatcaatg atttctgtga
     3361 aaaagccagt ttatggggag gcgtctcatt tcctatcagc agagcggttc tcagagctct
     3421 gattctggca ctatcagtgt ttggcaatcg gcggtatggc atttgaaaaa gtgttgaaaa
     3481 gatgttaata actgatgcga atataaaaat attttgttaa taacaaattc tcttcgcatt
     3541 aattaacaaa aaaatttaaa taacttgttt catcacacag gggttattac cttagcctga
     3601 aaataaaatt aaaaaagcag aatcatgttc agcagtgaag tttattcaga cagacggcag
     3661 aaactgaggt ctctcgttaa gtcggggctg gcgtttttcc ctggaaatgt tgaatcgcca
     3721 gttaactatc cagctaatcc ttatcatttc aggcaggaca gcagttttct ttattttttc
     3781 ggactcgacc ttcccggact cgccggggta attgatttcg acagcggaga tgaatggatt
     3841 tttggtgatg attttgatct gaatgatatt gtatggatgg gaccacaacc aacggtaagg
     3901 gagcttgcca tgaaatgcgg tattgtaaaa acagcccctt tgaagaagct tgaagaaata
     3961 atagctgatg cagtttctaa gaaacgtaaa atacattttc ttccaccata cagaggtgaa
     4021 acgaaaatga tgcttggagc tcttttaaag gaggatccct gtaaaatgaa gacgcttgca
     4081 tcagaagaac tcatcagagc agttgtatct ttgcgaataa taaaagaagc aattgaaatt
     4141 gaggagattg agaaggctgt aaatatttca tgggaaatgc atgttacagc aatgaagatg
     4201 tgcagggctg gggtaaagga gcaggagatt tttggtgtta tagaaggtat agcatggtcg
     4261 aagggtgctg gtccggcttt tcctgtgata cttagcataa acggtcagac acttcacaac
     4321 catgcacaca acaacatact tactgaaggc aggatgatgg ttactgatgc aggagcggaa
     4381 tctctcttgc attatgcttc agatataaca cgtactacac ctgttggagg caaattcaac
     4441 agcaggcaaa gggacatcta tgaagttgtg ctccgggcaa ataatcttgc tattggtgaa
     4501 gcaaaaccag gtaagtcgtt ccgtcagata catattgatg cttgcaaagc aattgcacag
     4561 gggatgaaag aactgggact gatgaaaggt gataccgaag aggctgtgat gcagggtgca
     4621 catgctctgt ttatgcctca tggtctgggt catatgctcg ggctcgatgt gcatgatatg
     4681 gaaggattgg gtgaaaacta tgtagggtat aacgacagga caaaacgcag tgaccagttt
     4741 ggtctctcat ttctgaggtt cgctctcgaa tgcaaacccg gccatgtaat cacaattgaa
     4801 ccaggttgtt attttattcc tcagctgatt gaccagtgga agtctgaagg caaattcaaa
     4861 gagtatatat gttatgacag gcttgaatcg taccgggatt ttggaggaat aaggattgag
     4921 gataatattg caattacaga aacagggtgc cgtctgcttg gcagacctat tccgaaaacg
     4981 gttgaagaga ttgagtcggt atgttcctaa tttctgccgg tattagtgcg tactggttga
     5041 gattgagttt gtcggactgc acgtcccgga aaaactgcga tactttcatg acctgaagcg
     5101 tcaaccgact gaattgcaaa gaagtaattg tcttttgaat agggcagggt aacttctgta
     5161 cctgttacaa atatcttctt atcccatagt ggttgatatg tttctcttat cagtacataa
     5221 tatccggccg gttttattcc ctttgaaggt tcttcccatc tcagggttgt agtgttgctc
     5281 aggccggcag ttacgatgcc gcagtttacc ggttcataag gtgcgagggc cagattagca
     5341 attgttgcta gatttatgcc agtgtttttt cttacgtatt cataatcgac atattcagga
     5401 agatctccct gctgtattcc atttacaagg gcagggacct tatgagttct atcgtagttt
     5461 tcgttgaact cgcatattct aacagcagga aagccttgct ggctgaaagg agtgtgatcg
     5521 ccaccgcgtc cgaaacggtc gttacggaat accagggtaa cgtttatctg atcaacatag
     5581 cgttctccta tttctttgat atatcgagca agctgacggg ccctgctatc attctcaccc
     5641 gaggtatatc ttctcagggc agccatctgt tcggtctcaa atgctggtac cccctcactg
     5701 aaaaccctta ccctcatatt atcattaaga agtgtttcac ttgaaccgct attgccaatc
     5761 atatcgttat taagcatagc tattatgttc cagttttcct gtttggcttt tgctgccata
     5821 aaccttgatc cataaagacc gtgttcctca cccgagaaag caataaatac aattgtggct
     5881 gaaaatttca caggagacat tattctcagc atctcgagaa gtgccgcaat accggaggca
     5941 tcatcatcgg caccgggggc gaagcttgta aaatcattgt cagcttctgc ccgggaatca
     6001 aagtgggctc ctatcacaat taccctgtca tcatcgggat taattccctt gagtgttgca
     6061 actacatttt tcaataaaat ttcttgaggt attctctgcc cttcaccacc tgctttatac
     6121 tcttcaaacc tcaccgaaag ccttccaccc gattggggaa tattcttttc catctcagct
     6181 tttatccagt tccatgctgc acctatccct ctttcagggt tattttgctc actcatgttg
     6241 tgacgggtat gaaaactgac aagttttctt acgtataatt caagattttc ccctgaaatt
     6301 gcatcaacca tcttcctgat gagggggtcg cggttgacaa tggtctgaga tgtggcatga
     6361 aataatgaca taattaaaaa gaaagttatg aaaatttttt tcatgtgtaa ataatttatt
     6421 tttaatggta ccatggagcc tccatgtttt gaaaaattat tttcataatt ttttgtgttt
     6481 aacaaacaca tggaagtttc ataaacatct gtcgttaata ggctttgtaa tattctctgt
     6541 ttagtctggc gatatttgag attttgattt ttttcgggca ttctgcttca catgcacctg
     6601 tgttagagca gtttccgaag ccaagttcat ccattttttc caccattgcc cttactcttt
     6661 cttttgcttc aattctgccc tgaggtatta gggcaaactg tgatattttt gcggagacaa
     6721 aaagcattgc agatgcattt ttacaggctg ctacacatgc cccacagcct atgcatatag
     6781 cggcgtcgaa agccatatca gcattttgtt tcctcacagg tattgaattt gcttcgggtg
     6841 ccgagcctgt atctactgaa atgaagcctc ctgcaatttg tattttatca aaagcgctac
     6901 gatcgacaat aaggtcttta ataaccggaa agggctttgc acgccagggt tcaacccata
     6961 gagtttcgcc gtcattgaag tacctcattg aaagatggca ggttgtaata cccggaacag
     7021 gaccatgtgg atgtccgttg atatagatac cgcacgaacc gcaaattccc tcgcggcaat
     7081 cgtggtcaaa agccaccggg tcgccacctt cctcaacgag ccttttgttt aaagcatcga
     7141 gcatctcaag aaacgacatt tcgggcgaaa cattttccat gtggtatgtt tcaaaccgtc
     7201 cctttgattt gctatccttc tgcctccata ttttgagcgt aagcttcata atccggatta
     7261 ttaattaatt tctactcttc atgtaaaatt atttcttgtc ttatttctca gggcgtagag
     7321 cacaaagtat caggtaatta gtgatattgg tattcagtaa tcggataatt gaatacttat
     7381 tgtcggaact gaaaaccctt cctgaatgct catgctccat aacccagact tttatttata
     7441 actcctctct ttcatctcta ctacttcaaa cttaagttct tctttatgta gcacaggggg
     7501 attaccgtga ccggtatatt cccatgcagc cacgtatgcg tattctttat catttctcag
     7561 aggttcgcca tcgggtgtct ggtattcctc cctgaaatgt gcgccgcaag attcttttct
     7621 attaagagca tctataacca gtaattccgc cagctccagg aaatcagcca ccctgcctgc
     7681 tttttcaagt tcctggttca gtccgtttat gcttccaggt actttaaggt tcttccagaa
     7741 ttcctccctc agctcacgaa tctgcgacaa tgcttttgta agtccctcct cattgcgcac
     7801 catccctaca taatcccaca taattttacc gagcctctta tggaatgatg atgctgtttc
     7861 tcttccgtta actgcgagca gtttttcaag tctttcaata gaactccttt cggattcttc
     7921 aaattctgga aggtttacag gtatgcgtgg tgtttttatc tggtcagcaa gatagttgct
     7981 gattgtgtta ggaagtatga aataaccgtc tccagcagcc tgcatcaatg agcttgcacc
     8041 aaggcggttt gcaccatgat cagaaaaatt gcattcgcct atggcgtata atccgggtat
     8101 tgttgtcatc agctcataat caacccacag tccgcccatt gtataatgac cggctggata
     8161 aatcatcatt ggctcctcgt aagggtcttt accggtaatg gttttgtaaa gctcgaacag
     8221 atttccgtat ttgtcttcaa ttgctttttt accctgcttc tttatggcat ccctgaaatc
     8281 gagatagact gccagaccag ttttgcccac tccgtaacca gcatcacacc tttcctttac
     8341 agctcttgag ccgacatcgc gaggcacgag gtttccgtat gcaggatatc ttctttcgag
     8401 aaaataatcc ctctcctcct caggtatctc attgggattt cttgtgtctc cttttgtttt
     8461 aggaacccac atccggccat cgtttcgcag cgactccgac atgagtgtga gctttgactg
     8521 atagtcgttc aactgtggca cacaggttgg atgtatctgt ataaaacttg gattggcaaa
     8581 gagggcacca cgtttgtagg ctttccaggc tgccgacggg ttggaattta ctgcaagggt
     8641 tgaaagataa tatatagttc cataaccgcc tgttgcaaga acaacggcat gg
//
LOCUS       DAOSNB010000077         8626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8146_length_8626_cov_4.291448, whole genome shotgun sequence.
ACCESSION   DAOSNB010000077 DAOSNB010000000
VERSION     DAOSNB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8146_length_8626_cov_4.291448"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="PLG42_04610"
     CDS             <1..1492
                     /locus_tag="PLG42_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOU30528.1"
                     /translation="KAFCEAVGRLSNYAHTEVFGRTKEGNDLIIVVLANPKVTSPAEA
                     AATGKPVIYIQGNIHAGEVEGKEACLQLTREICFGTKTPLIDNQIIIFCPNYNPDGND
                     KLSATSRPSQDGSPLLTGARVSGEGYDLNREGIKLEAIEAKAFMKNIILRWDPALLID
                     LHTDNGSWHGYTINYAPCYKSAGLDIITDYVKDKMLPAATLSMTNRSGIPIWFFGNLT
                     QKPGELPVFSTYSHLPRYIVNYMGLRNRMAILSETFSHDSFEKRVLSNYLFLVSILEF
                     TNSNATEIKKIIAAADAETIKTISEKGGIIERGVTYRIAEADKPFPLLMRETEEYKSE
                     DGRVRRRATGKLYWVDSVRYFDHFLPDKTAKVPYGYIFPAELKGIADKLQEHGINVTV
                     IEKRKVFEGEQFIITKYAKETRAIYGGHNPVRVEGDFRKGKFTADKGSYYVDMRQPLA
                     WLVFYMLEPESDDGLLFWNFFDEYLTLKGVENGNIPYPVLKILKPY"
     gene            complement(1584..1667)
                     /locus_tag="PLG42_04615"
     tRNA            complement(1584..1667)
                     /locus_tag="PLG42_04615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1631..1633),aa:Leu,seq:gag)
     gene            complement(1690..1773)
                     /locus_tag="PLG42_04620"
     tRNA            complement(1690..1773)
                     /locus_tag="PLG42_04620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Leu,seq:cag)
     gene            2225..3547
                     /locus_tag="PLG42_04625"
     CDS             2225..3547
                     /locus_tag="PLG42_04625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU30529.1"
                     /translation="MKIMKFGGTSVGTAERMRAIVPLINTGEKVIVVLSAMSGTTNAL
                     IEISSALYAGNIKEAGSKIESLRAKYLDVVNNLYTHEETRQTGREIIDNHFNYISGFI
                     NGSFTRTQEKAIVAQGELLSTTLFHLLLQEQNINSALIPALNFMRIDKYGEPDIYYIT
                     ENLKRELENYPDTNIIITQGFICRNAFGEIDNLKRGGSDYTASLIGAALDASEIQIWT
                     DINGFHNNDPRYVENTFPVRELSFDEAAELAYFGAKVLHPSTVNPARDKNIPVRLKNT
                     MEPDDPGTLISRKQTFQDYKAIAAKDGITAIRIKSDRMLMAYGFLRKVFEIFEVYRTP
                     IDMITTSEVSVALTIDNPENLPDILKELQNLGSVEVEENQSIICIVGDFRPETTGSAP
                     EIFEALNTLPIKMIAYGGSPYNLSLLVDTKNKVQALRLLNKHLFKEQK"
     gene            3544..4689
                     /gene="lysA"
                     /locus_tag="PLG42_04630"
     CDS             3544..4689
                     /gene="lysA"
                     /locus_tag="PLG42_04630"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOU30530.1"
                     /translation="MTTLFPVNRFKELRTPFYYYNTSVLKKTIELLKEETDKSGFKVH
                     YAIKANANPVLLKIISSYGLGADCVSWNEIERALSCGFRPDGIVFAGVGKSDYEIENA
                     LKADILCFNAESLQELSVINEIAGKLGKTARIALRINPNIEAHTHKHITTAIDDSKFG
                     IIIPELDEALNLISGAKHLQLIGIHFHIGSQITRMQVFKNLCARINELTEWFSSRNVS
                     LKIINVGGGLGIDYHEPDILPAFREYFSVFREMLRLYPGQELHFEPGRAIVGQCGTLI
                     SKVLFTKNGGNTKFAVIDAGMTDLIRPALYQAYHKIENLTSNGEKTHYDVVGPICESS
                     DTFGKYVELPETKRGDIIAIRSAGAYGESMASRYNLRDLPPAVFSED"
     gene            complement(4686..6443)
                     /locus_tag="PLG42_04635"
     CDS             complement(4686..6443)
                     /locus_tag="PLG42_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOU30531.1"
                     /translation="MMQSINDFLVMLDGYIGGHPWFVCLLLGTGIFFTFYLRFPQFRF
                     FRHAIDIVRGKYDHHLDVGDTSHFQALSTALSGTVGTGNIAGVALAIHLGGPAALFWM
                     MVTAAIGMCTKMVEVTISHKYRDILPDGTVSGGPMYYMKKRLNIKTKSGKIIRTGAII
                     GAFFAVATVLSSFGTGSLPQINSISNAVFATFGINHIITGAILAFLLALIIIGGIKRI
                     AQVTEKLVPGMAIFYFIGALLVIFTNYHNIIPSIISIFTTVFTGTAAVGGFLGATVAY
                     AINRGVNRGLFSNESGQGSAPIAHASAKAPEPVSEGLVALLEPFIDTIIICFLTGLTI
                     LSSGVWHEKVENQFQSTDMIFLNDFYDEKIPEQKQKVIEFVRDKKPLPLFNGKLIVNE
                     GKIQNKITLIASRSIAEDYTVYENKKPYTGEIQVVNGKWQPSSPSIYIVGKSLVHSAP
                     LTTEAFKRSILGDWGKYVVAIGLLLFAFSTAISWSYYGDRAITYLLGPQYVIYYRIIY
                     VVAFFIASFTDTTIVWSLSYITIALMTVPNLIGLWILRKEIKQTIDAYWTDLLSKHPE
                     DKKLLHYVKRDMLRKKNKG"
     gene            complement(6613..7977)
                     /locus_tag="PLG42_04640"
     CDS             complement(6613..7977)
                     /locus_tag="PLG42_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOU30532.1"
                     /translation="MSGSGTEKRDSFGSNIGAIAAIAGSAIGLGNIWRFPYVAGENGG
                     GAFLLIYIVFILLIGIPVMTSEFIIGRSAQKNPYGAFRLLAPGKPWFLIGVMGVLAAF
                     FILSFYTTVSGWTLEYFYQAITGNLSGKTNRELAEMFTGFLNSPFRPYLWFLIFMGLT
                     ALIIISGVKNGIERYAKILMPFLLVLLVILCIRAITLKGANEGLRFLFHPDVSKITPK
                     VILEALGQGFFSLSIGMGTLITYGSYIRKKDNLASTALYVTASDTIIAILAGVAIFPA
                     VFALGGTPASGSGLTFIVLPEVFQKLPFGNVIAMIFFILLAIAALTSTISVLEVIVAF
                     LVEELKMVRRKATIVASVSVSVLGLLTVASMGKLGSFTILGKNIFGLLEYLTANIMLP
                     LGGLLIVIFIGWFHDKNLTKLEMTNNGEIKSWYFPVYFFLVRYIAPVAIALVFLYSLG
                     IINF"
     gene            8169..>8626
                     /locus_tag="PLG42_04645"
     CDS             8169..>8626
                     /locus_tag="PLG42_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOU30533.1"
                     /translation="MVKLTFPDGTVKEFTEGITGLEIARQISPQLAKEVLSVSVNGEL
                     YDLTRPITTDANIKLHKWEDEEGRHAFWHSSAHLMAEAIEQLWPGTKFGIGPAIENGF
                     YYDIDPGDHEITDDDLISIENRMKELASQNQQIIRKEVSKKEALEFFEKKG"
BASE COUNT         2863 a         1694 c         1699 g         2370 t
ORIGIN      
        1 aaaagctttt tgtgaagctg tcggccgact gagcaactat gcccatacag aagtattcgg
       61 caggaccaag gaaggtaatg acctgataat tgttgtgctt gcaaacccga aggttacatc
      121 tcctgctgaa gcggccgcga caggcaagcc tgttatttat atccagggaa acatacatgc
      181 tggagaggtg gaaggcaagg aagcctgcct gcagctgacg agggaaatat gtttcgggac
      241 taaaaccccg ttaattgaca atcagataat tatattttgt cctaattata atcctgatgg
      301 caacgacaaa ctaagtgcca caagccgacc ctcccaggat ggcagtccgc tactaacagg
      361 agccagggtt tccggcgaag gttacgacct taacagggaa ggcataaaac tcgaagccat
      421 tgaagctaag gcattcatga agaatataat cctgagatgg gatcctgcat tgctgataga
      481 ccttcacacc gataacggct catggcacgg atatacaata aattatgcac catgctacaa
      541 aagtgctggc ctcgatatta taacagatta tgtaaaagac aaaatgcttc ctgcagcaac
      601 tctgtcaatg accaacagat cgggaatacc catatggttt ttcggtaatc ttacccagaa
      661 gccgggagaa ctgcctgttt tttcaacata ttctcatctg ccaagatata ttgtgaatta
      721 tatgggactg aggaacagga tggcaatttt gagcgaaact ttttctcacg atagctttga
      781 aaaaagagtg ctcagtaatt atctgttcct tgtttccatt ctggaattta caaattcgaa
      841 tgctactgaa attaagaaaa taatagctgc agcagatgct gaaactatta aaacaatttc
      901 tgaaaaaggc ggaattatcg aaagaggagt tacctacagg atagctgagg ctgacaagcc
      961 ctttcctctg cttatgaggg aaactgaaga gtacaagagt gaagacggaa gggttaggcg
     1021 cagggcgaca ggaaaacttt actgggtcga ctctgtaaga tattttgatc attttttgcc
     1081 cgataaaaca gcaaaggtac cgtatggata tattttcccg gcagagctga agggtattgc
     1141 agacaagtta caggaacacg gtattaatgt tactgttatt gaaaaaagaa aagtatttga
     1201 aggtgagcaa tttattataa caaaatacgc aaaagagaca agagctatct atggtggaca
     1261 taatccggtg agagttgaag gcgatttcag aaagggtaaa ttcactgctg ataaaggtag
     1321 ttattatgtt gacatgagac aaccgcttgc atggcttgtt ttttacatgc tggaacctga
     1381 atcggacgac gggcttctat tctggaattt ttttgatgaa tatcttaccc tgaagggggt
     1441 cgaaaacggc aatatcccct accctgtatt aaaaatatta aaaccatatt gatcaggata
     1501 atacaaaaaa gaaaaaggag aaaaatatca tccctaaaat aatcaggatt aaattttctc
     1561 ctttttcttt tgttcttatt tagtgcccag gacaagactc gaacttgcac accgtttccg
     1621 gcactacccc ctcaaagtag cgtgtctacc aattccacca cctgggcata ttgtttgcct
     1681 ttccttgtgt gcccagaaca ggactcgaac ctgcacatca ttgctgatac tagtccctga
     1741 aactagcgcg tctaccaatt ccgccatctg ggcatacaga ttataaataa aatacaactt
     1801 taatctgtgc ctttcttttg ttttaaaatc aggtacttcc tattccaggc atcactattt
     1861 caatgaaccg accaagtacc cgattccggg attgcaaaaa tataaaaatt tgaaaaattt
     1921 cacaaggttg acatatttta tatttatgat ttatccttaa gaccgcccag gctccactat
     1981 gaatctaccg cttcccccgg acagagcgaa aatctattaa ctggagaatt atacatacaa
     2041 tagttatgct tactttgtta tgtatttaaa aaatttactg caaatagata atccattaaa
     2101 taaaaaatat atttttgctc cctattttat aaaaaagaag atatttatta aaatttgcag
     2161 ttgttttaat aaacatcaat atatcagact aatttaatta caaatttaaa tttaacaggt
     2221 gcaaatgaaa ataatgaaat tcgggggaac ttcagtaggt actgctgaaa gaatgcgagc
     2281 aatagttccc ctgataaaca caggtgaaaa agttatagtg gttctctcag caatgtcggg
     2341 aacgacaaat gctctcattg agatttcctc tgccctttat gccggaaata taaaggaagc
     2401 aggttctaag attgagtcac tcagggcaaa atatcttgat gtagtaaata atctttacac
     2461 ccatgaagag accaggcaga caggccgtga aataattgat aaccacttca attatatttc
     2521 gggtttcatt aacggttctt ttacaaggac tcaggaaaag gcaatcgtag cgcaggggga
     2581 actcctttca acaactcttt tccaccttct cctgcaggaa cagaacataa attcggccct
     2641 tatacctgca ttgaatttca tgcgtatcga caagtacggt gagcctgata tatactatat
     2701 caccgaaaat ctcaaaaggg aactggaaaa ttatcctgat accaatatta ttattaccca
     2761 gggatttata tgcagaaatg cttttggaga gattgacaat ctcaaaagag gaggtagtga
     2821 ttatactgca tcacttattg gagcagcgct cgatgccagt gagatacaaa tatggactga
     2881 tatcaacgga tttcacaata acgatccaag atacgtggag aatacctttc ccgtcaggga
     2941 actttctttt gacgaagctg cagagcttgc ttatttcggg gcaaaggtac tccacccatc
     3001 aacagtgaat cctgcacgtg ataaaaatat tccggtacgc cttaaaaaca caatggaacc
     3061 tgatgacccc ggaacactga tatcacgcaa acaaactttc caagactata aggcaattgc
     3121 agccaaagat ggaataacag ctataagaat aaagtcggac aggatgctta tggcttacgg
     3181 tttcctcaga aaagtgtttg agatattcga ggtatatcgt acccccattg acatgatcac
     3241 aacctctgaa gtttcagttg cccttacaat agataatcct gaaaacctcc cagacatatt
     3301 gaaagagcta cagaacctgg gctcagttga agttgaagaa aatcaatcaa ttatttgcat
     3361 agttggagat tttcgaccgg agacaacagg ttcggcaccg gaaatattcg aagctcttaa
     3421 caccttacca atcaaaatga tagcttatgg aggcagccca tataaccttt cacttcttgt
     3481 agacacaaaa aataaagttc aggctttaag attgcttaat aaacaccttt tcaaagaaca
     3541 aaaatgacaa ctctatttcc ggttaacagg ttcaaagaac ttcggacacc attttattat
     3601 tataatacaa gtgtcctgaa aaaaactatc gaactgctta aagaagaaac tgataagtca
     3661 gggtttaaag ttcattatgc tattaaggca aatgcaaatc cagtgttgct gaaaattatt
     3721 tcatcatatg gattgggagc cgactgcgta tcgtggaatg aaattgaaag ggctttatcc
     3781 tgcggtttca gacccgatgg aattgttttt gccggtgtcg gaaaatcgga ttacgaaatt
     3841 gaaaatgcgc taaaagcaga tattttatgt ttcaacgctg aatcgcttca ggaactgtca
     3901 gtgataaatg aaatagccgg aaaactggga aaaactgcaa gaatagcact tagaataaac
     3961 ccaaatattg aagcacacac acataaacat ataacaactg caattgatga cagtaaattc
     4021 ggaataataa taccggaact cgatgaggca ttaaacctga taagtggagc aaagcacctc
     4081 cagttgatag ggattcattt ccacatcggt tctcagataa caaggatgca ggtatttaag
     4141 aacctgtgtg caagaatcaa tgaacttacc gaatggttca gttcacgaaa cgtttcactt
     4201 aaaataataa atgttggtgg tggcttagga atagattatc atgaaccaga cattttgcct
     4261 gcattcaggg aatatttttc agtattcagg gagatgctca ggctttaccc gggacaggag
     4321 cttcactttg aaccgggaag agctattgta ggacaatgtg gaactctgat ttcaaaagtt
     4381 cttttcacaa aaaatggcgg aaatacaaaa tttgcagtta tcgatgctgg tatgaccgac
     4441 ctgatacgtc ctgcattata tcaggcatac cataaaattg aaaacctgac ttccaacgga
     4501 gaaaagaccc attatgatgt tgttggtcca atatgtgaat cgtccgacac attcgggaag
     4561 tatgtcgaac ttcctgaaac aaagcggggc gacattatag caataaggtc agcaggagca
     4621 tacggcgaat caatggcttc aagatataat ctgagggatc taccccctgc agtcttttcg
     4681 gaagactaac ctttgttttt cttcctcagc atatctctct ttacataatg cagaagcttc
     4741 ttgtcttcag gatgcttgga aagcaggtcg gtccagtaag catcaattgt ctgtttgatt
     4801 tctttcctca gaatccataa tcctatcaga ttcgggactg tcatcagggc aattgtaatg
     4861 taggagagtg accatactat cgttgtatcc gtaaaagaag ctatgaagaa agcaactaca
     4921 tatataattc tgtaatatat aacatattgt ggcccgagaa gatatgtaat tgccctgtcg
     4981 ccatagtacg accacgaaat tgctgttgaa aacgcaaaaa gaagcaaccc aattgcaaca
     5041 acatatttac cccaatcacc aagaatactt cttttgaatg cttcagttgt aagaggagca
     5101 ctatgcacaa gagatttacc aacaatatat atagatggag aagaaggttg ccatttcccg
     5161 tttaccacct gaatttctcc cgtatagggt tttttatttt catacacagt gtaatcctct
     5221 gcaattgagc gcgatgcaat aagagtgatt ttgttctgga ttttgccttc atttacaata
     5281 agttttccgt tgaaaagtgg caaaggtttt ttatctctta caaattcaat tactttttgc
     5341 ttctgctccg ggatcttctc atcataaaaa tcattgagaa atatcatgtc ggtgctctga
     5401 aactgatttt cgactttttc atgccagaca ccagatgaca atatcgtcag gccagtaaga
     5461 aaacatatta taatggtatc aataaatggt tcaagaagtg ctaccagtcc ttccgatacc
     5521 ggctcaggag ctttggctga agcgtgagct atgggtgcag agccctgtcc ggattcattg
     5581 gaaaaaagtc ctctgttaac tcccctgttg attgcatagg ccacagtagc accgagaaaa
     5641 ccgcctacag cagcagtacc ggtaaaaaca gttgtaaata tggaaattat tgagggtatg
     5701 atattatggt aattcgtaaa aattaccagt aaagcaccga taaaataaaa tattgccata
     5761 ccaggtacca gcttttctgt tacctgggct attcttttaa ttcccccaat aataataagt
     5821 gcaagcaaaa aagccaggat agcacctgtt atgatatgat ttattccaaa tgttgcaaat
     5881 acagcattgg aaatactgtt tatctgtgga agactgcctg ttccgaatga agaaagaacc
     5941 gttgcaacgg caaaaaatgc accgattatt gcgccggttc tgatgatttt cccggatttt
     6001 gttttgatgt taagcctctt tttcatgtaa tacattggac cgcccgagac ggtaccgtca
     6061 ggcagaatat cacgatactt gtgagagatg gttacttcta ccatttttgt acacatacct
     6121 attgctgcag taaccatcat ccagaagagt gcagccgggc caccaaggtg aatggcaagt
     6181 gcaactcctg caatattgcc tgtccccaca gtacccgaaa gggctgttga aagtgcttga
     6241 aaatgtgatg tatctccaac atcaagatga tgatcatatt tgcccctcac aatatcaatt
     6301 gcatgcctga aaaatctgaa ctgcggaaac ctcagataga atgtaaaaaa tattccggtt
     6361 ccaagtagaa gacaaacaaa ccacggatgc cctccgatat atccgtcaag cattacaaga
     6421 aaatcattga tactctgcat cataatttaa tagtgtaaga aaaacacaaa tgatgattgg
     6481 caaatgtaac aatttccaga taagaaaaaa gcctgaagca aatgttaaaa attcttattt
     6541 tcatcaatat caatagatat cagcttttga gtaatgttgc cttgatgcaa cataatggtg
     6601 aatctgaaaa attcaaaaat ttataatgcc caggctataa agaaacacta gcgcaattgc
     6661 aacaggagcg atatatctta caaggaaaaa atatactgga aaataccatg attttatctc
     6721 accgttattc gtcatctcaa gttttgtaag gttcttatca tggaaccatc caatgaaaat
     6781 cactattaac agtccgccca ggggaagcat tatgtttgcc gttagatatt ccagaagtcc
     6841 gaagatgttc tttccgagaa tggtaaaact gcccagttta cccattgatg ccacagtcag
     6901 aagtccgagc accgatacag aaacagaggc tactattgta gcttttcgtc tcaccatttt
     6961 tagctcctct acaaggaatg caacaattac ttccagtacc gatattgttg aagtgagtgc
     7021 agctatggca agaagaataa agaaaatcat tgctatgaca ttgccaaaag gcagtttctg
     7081 aaacacttcg ggaagaacta taaatgttag tccagaacct gatgcaggag tgccgccaag
     7141 tgcaaatact gcaggaaaga tagcaactcc ggcaagtatt gctattattg tatcagaagc
     7201 tgtcacataa agagctgttg aggcaagatt atcttttttc cggatatatg aaccataagt
     7261 aataagagtt cccatgccta tgctgagtga gaaaaatccc tgtccgaggg cctccagtat
     7321 aacttttggt gtaatcttgc ttacatcggg atgaaagaga aagcgtaacc cttcatttgc
     7381 accttttagt gttatagcac gaatgcacag gatcacaagg agaacaagaa gaaaaggcat
     7441 aagtattttt gcataacgtt ctattccgtt tttaactccg gatataatta tcaaagctgt
     7501 taaccccata aaaataagaa accacaggta aggtctgaac gggctgttaa gaaaacctgt
     7561 gaacatttct gcaagttccc tgtttgtttt ccctgaaaga ttgccggtaa ttgcctgata
     7621 gaaatattca agggtccagc ctgaaactgt tgtataaaac gacagaataa aaaatgctgc
     7681 cagtacaccc atcacgccta taagaaacca tggtttgcca ggagccagaa gcctgaaagc
     7741 accgtatggg tttttctgtg ctgatcggcc aatgataaac tctgaagtca ttaccggaat
     7801 gccaatgagc aaaataaaaa caatgtatat caacagaaat gcacctcccc cattttcacc
     7861 tgcaacatac ggaaatctcc agatgtttcc taaacctatt gccgaaccag caattgcagc
     7921 aatggcacct atattacttc caaaactgtc acgtttttct gttcctgacc cactcatggt
     7981 ttttaattta tttcattatt gatttattaa taagaatatg gccgctaata taaaaattat
     8041 tgagcaacct gatttgtttt taatattctt caaagaatta aaaaaggtta acagttttcc
     8101 tgaaacaccg gacaaaaaag attttaaaat atgtttatct ttgcactctc aaaattaaaa
     8161 acataataat ggtaaagctc acttttcctg acggtacagt aaaagaattc acagaaggaa
     8221 taactggact ggagattgcc aggcagataa gtccgcagct tgcaaaagaa gttctttctg
     8281 tgtcggttaa tggggaatta tatgatttaa caaggcctat cacaacagat gcaaatataa
     8341 agcttcacaa atgggaggat gaggaaggca ggcatgcctt ctggcactca tcggcgcatc
     8401 ttatggcaga agcgattgag cagctctggc ccggcacaaa atttggtatt ggtcctgcca
     8461 tagaaaacgg tttttattat gatatcgacc ccggtgacca tgaaataaca gacgacgacc
     8521 tgatttctat agaaaacagg atgaaagagc ttgcatctca gaatcaacaa attataagga
     8581 aagaagtaag taaaaaagaa gcactcgagt ttttcgaaaa gaaagg
//
LOCUS       DAOSNB010000078         8589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8180_length_8589_cov_3.964964, whole genome shotgun sequence.
ACCESSION   DAOSNB010000078 DAOSNB010000000
VERSION     DAOSNB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8180_length_8589_cov_3.964964"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLG42_04650"
     CDS             complement(<1..476)
                     /locus_tag="PLG42_04650"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOU30534.1"
                     /translation="MKSDIEIAQEAPIRHILEIAEQLGISKSDLELYGKYKAKIPLSY
                     IDEEKASKANLVLVTAITPTPAGEGKTTTSIGLAQGLNRIGKKTTVVLREPSLGPVFG
                     IKGGAAGGGYSQVIPMEDINLHFTGDMAAVEKAHNLLSALIDNNIQQNIVRVVSLS"
     gene            complement(602..>1783)
                     /locus_tag="PLG42_04655"
     CDS             complement(602..>1783)
                     /locus_tag="PLG42_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOU30535.1"
                     /translation="ADPLFHAGCYYPQEASGMFLEEIFRQLFSGKKNLRVLDLCAAPG
                     SKSTHLSTLLADNGCLIANEVIRARVSVLAENITRWGIPNTIVTNNDPADFSKLKGYF
                     DFIVVDAPCSGEGMFRDPEARKEWSENNAALCSKRQKRIISEVWPALKENGILIYSTC
                     TFNPAENEENIKWLIDNTISECVRIDVSRFPQITEISYEGITGYGFYPYKSEGDGFFI
                     SVVRKKESSDVVKFRRNKKAIPVPAKIQKTAEKITGISAENIFMFNQIVYHTPFSTDE
                     MSFLSSMLKVIRPGTDLFRDIGGEIVPEHGLALTVLLKKGVYPMIELNYLQALSYLRK
                     DNIDVPLTGKGWYTVAYEGCNLGFVKSTGTRLNNLLPVQLRIRMSLEALSGKQTIKWC
                     M"
     assembly_gap    1784..1883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1884..2136)
                     /locus_tag="PLG42_04660"
     CDS             complement(<1884..2136)
                     /locus_tag="PLG42_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOU30536.1"
                     /translation="MFPEGFIKRLKTQKYIEADLLLESLRNPSPVSIRLNPLKWNRVP
                     VGALPVPWCSTGYYISERPSFTADPLFHAGCYYPQEASGM"
     gene            complement(2186..3244)
                     /locus_tag="PLG42_04665"
     CDS             complement(2186..3244)
                     /locus_tag="PLG42_04665"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOU30537.1"
                     /translation="MKSITVFSGLFLILASGICQQGGDVKGRVFTNPIFESGADPWII
                     YKDGFYYYTNTSGNRLFIRKARNLDELKNSEQKTIWTPPPGTSYSKEIWAPELHFIDG
                     KWYMYFAADDGNNWHHRMYVIENSSTDPTEGEWIFKGKVSDQSDKWAIDGSVFKYNGK
                     LYMIWSGWEGDVNGQQNIYIARMKNPLTIESERVMISAPEYDWEKVGDLNNPDDVPHV
                     NVNEGPVALVNGKKLFIIYSASGCWTDSYCLGMLTFTGKSDLLDPASWKKNPSPVFSS
                     KPEFNVYAPGHNCFFKSPDGKENWILYHANSAPGQGCGRYRSPRAQKFVFRKDGTPYF
                     GDPVKPGIPLPVPSEGEK"
     gene            complement(3315..4220)
                     /locus_tag="PLG42_04670"
     CDS             complement(3315..4220)
                     /locus_tag="PLG42_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU30538.1"
                     /translation="MKTDRRNFTRTLTLAGFSAFILGDPKIKTEQENTPVSKSALKIS
                     LNAYSFNSLLAAGKMTIDDMLDFCAETGIDGLDITGYYFPGYPEVPSDDYIFHVKKKA
                     FRLGIDLGCTGVRNDFTWSDPARRAEEKKLVKDWIVVAQKLGAPGVRIFAGTLSKENY
                     PWEERAKWIADDIRECAEFGKNHGVMLALQNHWDFIKTADEAEKMLKLIDHDWVGLML
                     DTGSYRSQNPYAEIEATAKYAISWQIKELVYINGKPENADIERIIRIIRKCGYRGYLP
                     IETLGEGDPFEKVKTLYSKVKKALG"
     gene            complement(4678..5541)
                     /locus_tag="PLG42_04675"
     CDS             complement(4678..5541)
                     /locus_tag="PLG42_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOU30539.1"
                     /translation="MKRIHNLITILLLSFLTAAFAYGSRKDKNSNSVIIITYNIRMNT
                     PADGENAWPLRKEKVSGLLRFHKSDIFNVQEALPEQMDDLSAFFPDFDHVGVGRDDGK
                     RAGEHMAIFYRRDRFQKIDDGMFWLSETPDRPGLGWDAACNRTVTWIKLKDNFTGKTF
                     YVFDTHFDHRGVKAREESAKMIITKIKEINRENLPLILTGDLNLEKNTPPVQLILSYL
                     DDAMDVTLAPHYGPMATSGGFEVKVLQRKIDYIFVNKKITVLRHGHLSDSFGLFYPSD
                     HLPVLAEVRIN"
     gene            complement(5633..7396)
                     /locus_tag="PLG42_04680"
     CDS             complement(5633..7396)
                     /locus_tag="PLG42_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOU30540.1"
                     /translation="MKKIYFLTLLLVVTFTGCELEQLPEATASRNAIFGSKEGLDLYV
                     NSFYTMLPNGNGCTTRDAMSDYLAVKTVPQFLQEAYSASNSSGWDWGDLRNINYFLKY
                     NVNPAIPADVREDYNGIAKFFRAYFYFEKVKRFGNVPWIGKPLDVSDTTILYGGRDPR
                     TLVMDSVLADINYACSNIKATSEPSRSLITKYVAYALKSRICLFEGTFRKYHTELGLE
                     NTANFWLNEAVTAAEAVMNSGIYSIYTTGGSTKAYRALFTSTSVITSEVMLAALMDQT
                     LNELHDANWYWTSGTYGDKASFIRSFINTYLKIDGTPFTDTPGWETMLFKDEVKNRDH
                     RLQQTIRMGDYKRIVGGVYVPAPPVFSYTFTGYQPIKWTLDDEYYDTRDLNVNDVPLF
                     RYAEVLLNYAEAKAELGTLTDADWANTIGKLRARAGITGGLTTKPTVVDTYLQSTYFP
                     DINDPVILEIRRERGIELCLEGFRFHDILRWKRGELMKMEWNGFYVPELVTPLDLNED
                     GILDVAFYQGTKPSPAVPGVTYVDVSDKPTNSQRLKNNTYGELTWMNNYPRVWDDKRY
                     YYPIPTSALITNPNLGQNPGW"
     gene            complement(7415..>8589)
                     /locus_tag="PLG42_04685"
     CDS             complement(7415..>8589)
                     /locus_tag="PLG42_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HOU30541.1"
                     /translation="PMILNNIRPQYTRFPNILPEGLTWETATTFDVGIDFSMFSGRFN
                     FSGDWYNRKTTDMYTIGLTLPAVFGATPPKGNYADLNTKGWELSVSWNDDFNVASKPF
                     NYNLRLALSDYRSVVTKYNNPDKLLTDYYPGMVIGEIWGYVTEGFFIDDEDVANHAKQ
                     SPEMRASPTNIWYAGDVKFKDLNNDGYINRGKNTVDDPGDRIIIGNRTPRYQFGINLG
                     ADWSNIFFSAFIQGVGKQDWYPSTECEFFWGQYNRPYNNVPKWHLGNMWTPENPNAYL
                     PRTMSRAASSTTSRELGVPQTKYLQNVAYVRLRNLQLGYSLPKQLISRIGAKDLKVYF
                     SGENLWDWSPLYKLIGNHLDVENTGPSDQEFTSGNAGDGYNYPMLMSFSFGLSITF"
BASE COUNT         2393 a         1781 c         1603 g         2712 t
ORIGIN      
        1 tttgaaagac tcacaaccct cacaatattt tgctgtatat tgttgtctat caatgccgaa
       61 aggagattgt gcgctttctc cacagcagcc atgtctcctg taaagtgcag gtttatgtct
      121 tccatcggta taacctgaga gtatccacct ccggcagcac ctccttttat accaaaaaca
      181 ggtcccaatg atggttccct cagaactacc gtggtctttt ttcctatacg gttaagcccc
      241 tgtgcaagac ctatggaagt tgtcgtttta ccttcaccgg caggtgtagg cgttatggct
      301 gtaacaagta ccagatttgc ttttgatgcc ttctcctcat caatgtaact caacggtatc
      361 tttgccttgt atttgccgta aagttcaaga tcactctttg agataccgag ctgctctgcg
      421 atttctaaaa tatgccttat aggggcttcc tgtgcgattt cgatatctga tttcatttga
      481 ttataaataa tagtgttctt ctttgttatc cattatattc cctttgcggt taaattactt
      541 ataaccatca ggtataataa ttgtggcact aaggtatata aaggaaatca aaaaaataat
      601 tttacatgca ccacttaata gtctgttttc cgcttaatgc ttccaacgac attcgtattc
      661 tcaactgaac cggcaacagg ttgttgagtc ttgtaccggt cgattttacg aaccccaggt
      721 tgcagccttc ataagctact gtataccatc ccttaccagt caggggtaca tcaatattat
      781 cttttctcag ataactgaga gcctgaagat aatttagttc aatcatcgga taaactccct
      841 ttttcagcaa tactgtcaga gcaagtccat gttcaggaac aatttcgcct ccgatatccc
      901 ggaaaagatc tgtgcctggc ctgatcactt tcaacatgct gcttaaaaaa gacatctcat
      961 cagttgagaa gggagtatga taaactatct gattaaacat aaagatattt tcagctgata
     1021 taccggttat tttttcagcc gtcttttgaa ttttggcagg aaccggaatg gcttttttat
     1081 ttcgtctgaa ctttaccacg tctgaactct ctttttttct gacaaccgaa atgaagaagc
     1141 cgtcgccttc cgacttatat ggataaaaac catatccggt tatgccttca tatgatattt
     1201 cggttatttg agggaaacgg gaaacatcta ttctgacaca ttcagatatt gtattatcta
     1261 tcagccattt aatattctct tcattttcag cgggattgaa tgtacatgtg ctgtaaatca
     1321 ggataccgtt ttctttgagt gcgggccata cttccgagat gattcttttt tgccgtttgc
     1381 tgcagagggc ggcattgttt tctgaccatt cctttcttgc ctccgggtcg cggaacatgc
     1441 cctcgcccga gcatggggca tcaacgacga taaaatcgaa ataacctttc agtttactga
     1501 aatcagcagg gtcgttgttt gtgacaattg tgtttgggat tccccagcgc gttatgtttt
     1561 cagcaagtac tgatactctt gctcttatca cctcgttggc tatgaggcat ccgttatcag
     1621 ccagaagggt tgataggtgt gtgctcttgc ttccaggggc tgcgcagagg tcaagaacac
     1681 gtaaattttt tttaccggaa aagagttgcc ggaagatttc ttcgaggaac atgcctgagg
     1741 cttcctgcgg gtaatagcag cctgcgtgga agagagggtc ggcnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnacatgcc tgaggcttcc tgcgggtaat agcagcctgc
     1921 gtggaagaga gggtcggcag taaaggatgg ccgttcagaa atataatagc cggtgctgca
     1981 ccatggcaca ggtaaagcgc caaccggtac ccggttccat ttaagcggat tgagtctgat
     2041 acttaccggc gaagggtttc tgagtgattc cagaagcagg tcagcttcaa tgtatttctg
     2101 ggttttcaga cgttttatga agccttctgg aaacatagat tcctttaact attttttaaa
     2161 aaacagaact cgaagcaatt acactttatt tttccccttc tgacggaact ggcaaaggga
     2221 ttccgggttt gaccgggtcg ccaaagtaag gggttccgtc tttcctgaat acaaacttct
     2281 gagctctggg tgaacggtat ctgccacatc cctgcccggg tgcagaattg gcatgatata
     2341 atatccagtt ttctttacca tcaggagact tgaaaaagca attatgtccg ggagcataaa
     2401 cattgaattc aggttttgaa ctgaaaacag gtgagggatt ctttttccat gaagccggat
     2461 caagaagatc acttttcccg gtaaaagtta acatgcccag gcagtaagaa tctgtccagc
     2521 acccacttgc tgaataaata ataaaaagtt tctttccatt taccagtgca accggaccct
     2581 catttacatt aacatgaggt acatcatccg gattattcag atctccaacc ttctcccaat
     2641 catactccgg agcagatatc attactcttt cactctcaat tgtcaatgga tttttcattc
     2701 ttgcaatata gatattctgt tgtccgttca catcgccttc ccagcccgac catatcatgt
     2761 agagtttccc gttatatttg aatacagagc catctattgc ccacttatca gattggtccg
     2821 aaacttttcc cttaaaaatc cattcccctt ctgttggatc ggtagatgaa ttttctatca
     2881 catacattct atgatgccag ttatttccgt catcagcagc aaaatacatg taccatttac
     2941 catcgataaa atgcaattcc ggagcccata tctcttttga atatgatgta ccgggcggag
     3001 gggtccatat ggttttttgt tcagaatttt taagttcatc aaggttcctt gcttttctta
     3061 taaaaagtct gtttcccgat gtgtttgtat aataataaaa tccatcctta taaattatcc
     3121 atggatcggc gcctgattca aaaatcggat tggtaaaaac tctgccctta acatccccgc
     3181 cctgctgaca tatccctgat gctaaaatga gaaataatcc tgagaatact gttatacttt
     3241 tcatttacat aacagtttgt atattaattt tctgccaatt aaataatgcc atcagacctt
     3301 aacttataga ttacttatcc gagggccttt tttaccttac tatatagcgt cttcactttt
     3361 tcgaacggat ctccttcacc aagtgtttcg ataggcagat aacctctgta accgcatttc
     3421 ctgattattc ttataattct ttcaatatct gcattctcag gttttccatt aatataaacc
     3481 agttctttta tctgccagct tatagcatat tttgcagtag cttcaatctc ggcataagga
     3541 ttctgcgagc ggtaactgcc ggtatcaagc atcagtccca cccagtcgtg gtcaataagt
     3601 ttaagcattt tttctgcctc atcagccgtt ttaataaaat cccagtggtt ttgaagtgca
     3661 agcataacac cgtggttttt cccgaattct gcgcattcgc ggatatcatc tgcaatccat
     3721 tttgctctct cctcccaggg ataattttct ttggaaagag tgcctgcaaa aattctaaca
     3781 ccgggggcac ccagtttctg ggcaacaaca atccagtcct ttaccagttt cttttcttca
     3841 gctcttctgg ccgggtcgga ccatgtaaaa tcgttgcgca ctcctgtgca gccaaggtct
     3901 atgccgagtc tgaaagcttt tttctttaca tggaaaatgt aatcgtcaga tggcacttcc
     3961 ggatagccgg gaaaataata tccggttatg tcaagtccgt caataccagt ttcagcgcaa
     4021 aaatcaagca tgtcatctat tgtcatcttt cctgctgcaa gaaggctgtt aaaagaataa
     4081 gcattaaggc ttattttaag agctgatttt gaaaccggag tgttttcctg ctctgtttta
     4141 attttcggat ccccgagtat gaaagcagaa aaacctgcca gggttagagt tcgagtaaag
     4201 tttcttctgt cagttttcat tatgtaaata ttttcagcaa tattcgacta aaataataaa
     4261 taataagagt aaattggggg gatgtatctt tttgataaat aattttttgc attttaagca
     4321 ttaatgctat agttacagtc tattttctga atgaataata attaatacta ttctcttcag
     4381 attttattat atagtcctct tctgattaac agtttattgt caataaaagt atggattgag
     4441 accatcctat ttactctgtc tgtccatcaa catactataa tcaagagcat aaccttaaaa
     4501 agaataagga ttaagaaatg aggttaaaaa aaacactata aaaacttcaa caggtaaacc
     4561 ctgccagctt actgaagaaa aaagcccaca ataaaaaaat aggccactaa ttcaaaaatg
     4621 gtagctgata ttgatgaact agtggcttaa atttagaagt gacagaaaag agtcacttta
     4681 atttattctg acttcagcaa gaaccggaag gtggtcagaa ggatagaaaa gtccgaatga
     4741 atcagacaga tggccgtgtc tgagaactgt aatcttctta tttacaaata tataatcaat
     4801 ttttctctgg agcaccttta cttcaaaacc gccgcttgta gccatgggac catagtgtgg
     4861 agcaagtgtt acatccattg catcgtcaag ataagaaagt atcagctgga ctgggggcgt
     4921 atttttttca aggttaaggt cgcctgttag aatcagaggc aggttttccc tgtttatttc
     4981 tttaattttt gttattatca tttttgctga ttcctcgcgt gctttcaccc cgcgatggtc
     5041 gaagtgtgta tcaaacacat aaaaagtttt ccctgtaaaa ttatctttaa gttttatcca
     5101 tgttacggtt ctattgcatg cagcatccca accaagtccg gggcgatcgg gtgtttcact
     5161 gagccagaac attccgtcgt cgattttttg aaacctgtcg cgccggtaaa atattgccat
     5221 atgctctcct gcacgtttac catcatcacg tccgacccca acatggtcaa aatcaggaaa
     5281 gaatgctgat aaatcatcca tctgttcagg aagagcttcc tgtacattaa agatatcaga
     5341 tttgtggaac cgcagcagac ctgatacttt ttcctttctc aatggccatg cattctctcc
     5401 atcggcgggg gtattcattc taatgttata agtaatgatt ataactgagt tgctattttt
     5461 atcctttctg cttccataag caaatgcagc agttaaaaag gatagaagaa gaatagtaat
     5521 taagttatgt attcttttca tggcattttt cacttttaat aaataggtaa caatcctgtt
     5581 tagattaaat aagcaggatt gttaccatat tataaatgta aaataaaaat tattaccaac
     5641 ccggattttg acccagatta ggatttgtaa tcaaagccga tgttgggata ggataataat
     5701 accttttatc gtcccacact ctcggatagt tattcatcca tgtaagttct ccgtaagtat
     5761 tgtttttaag tctctgtgaa ttagttggtt tatctgagac atccacatag gtaacaccgg
     5821 gaactgccgg tgagggtttg gttccctgat aaaatgccac atcaagaatg ccgtcttcat
     5881 tcaggtcaag tggtgtaaca agttcgggaa cataaaagcc attccattcc attttcatga
     5941 gttcacctct tttccatcgg agaatatcgt ggaacctgaa tccttcgagg caaagctcaa
     6001 ttccgcgttc tcttcttatt tccagaataa ccgggtcgtt tatatccgga aaataggttg
     6061 actgaaggta tgtgtcaact acggttggtt ttgttgtaag tccgccagtt attccggcac
     6121 gtgcacggag tttcccaatt gtattagccc agtcagcatc tgtcaatgtt cctaattctg
     6181 cttttgcttc agcataattc agaagtactt ctgcatatct gaagagaggt acatcattca
     6241 cgttaagatc tctggtatcg tagtattcgt catcaagagt ccacttgata ggctgatatc
     6301 ctgtgaatgt atatgagaaa acaggtggtg caggtacata aactccacct acaattcttt
     6361 tgtaatcacc cattcttatt gtttgctgta gcctatggtc tctattttta acttcgtctt
     6421 tgaagagcat tgtttcccat cctggtgtat cagtgaaagg ggttccatct attttaagat
     6481 aggtattaat aaacgaacgt ataaaacttg ccttgtcacc ataggtacca cttgtccagt
     6541 accagtttgc atcatgcagt tcattaagtg tctggtccat aagtgccgcg agcatcactt
     6601 cagatgttat gacagatgta cttgtaaata gagccctgta agcttttgtt gaacctcctg
     6661 ttgtatatat tgaataaatt cctgaattca tcacagcctc agcagcagta acagcttcat
     6721 taagccagaa attagcagta ttctcaagtc caagttcggt atgatacttt ctaaaagtac
     6781 cttcaaagag acatattctt gatttgagag cataggcaac atattttgtt ataaggcttc
     6841 ttgagggttc actggttgct ttaatattac tacatgcata atttatatca gcaagcactg
     6901 agtccataac cagtgttcta ggatcacgtc caccatagag tatagttgta tctgaaacat
     6961 ccagtggttt tcctatccat gggacgttac cgaacctctt aactttctca aaataaaaat
     7021 aagctctaaa gaattttgct ataccattgt aatcttcacg aacatcagcg ggtatagcag
     7081 ggttgacatt atatttgaga aaataattta tattcctcaa atcaccccag tcccatcctg
     7141 aactatttga agcgctgtag gcttcttgaa gaaactgtgg tacagttttt acagcaaggt
     7201 aatcggacat tgcatcccgg gttgtacaac cgttgccatt cggcagcata gtataaaatg
     7261 aattgacata taaatctagt ccctctttac ttccgaaaat tgcgtttctg gacgcagtag
     7321 cctcaggtaa ctgttccagt tcacaacctg taaaggtaac aaccagtagt aatgtcagaa
     7381 aatatatttt tttcataaca gtattattta aaaattaaaa agtgattgag agtccgaatg
     7441 aaaaactcat tagcatagga taattgtatc catcaccagc attaccagat gtgaattcct
     7501 ggtctgatgg acctgtattt tccacatcaa ggtggtttcc gatgagctta tacaatggtg
     7561 accaatccca gaggttttca cctgagaaat agaccttaag atctttagct cctattcttg
     7621 atatcaattg tttgggcaga ctgtaaccaa gctggaggtt acgaagtctt acataggcaa
     7681 cattctggag atattttgtt tgtggcacac ctaattctct ggatgttgta ctcgatgctg
     7741 cccttgacat tgtacgtggc aggtaagcat tcggattttc tggtgtccac atatttccca
     7801 ggtgccactt gggaacatta ttgtatggcc tattatactg tccccagaaa aattcacatt
     7861 cagtactcgg ataccaatcc tgtttcccaa caccctgaat aaatgcagaa aagaatatat
     7921 tagaccaatc ggctccaaga tttataccaa actggtaacg cggagttctg ttacctataa
     7981 taattctatc accagggtca tcaactgtat tttttcccct gttaatatat ccatcgttat
     8041 taaggtcctt aaatttaaca tctcctgcat accatatatt agtaggtgat gcccgcattt
     8101 ccgggctctg ttttgcatgg ttggcaacat cctcatcatc aataaagaat ccttctgtta
     8161 cataacccca gatttctcct ataaccattc ccggatagta atctgttagc aacttatcag
     8221 gattgttata tttggtcacc acagatctgt agtcagaaag tgccaatcga aggttataat
     8281 taaaaggctt tgaagcgaca ttaaaatcat cgttccatga aactgaaagt tcccaaccct
     8341 tggtatttag gtctgcatag ttacctttag gcggggttgc accaaatact gcaggaagag
     8401 taagtccaat agtatacata tccgttgttt tcctgttata ccagtcacct gagaaattga
     8461 acctgccaga aaacatcgaa aaatcaattc caacatcgaa agttgttgcc gtttcccatg
     8521 taaggccttc aggtaaaata ttagggaacc ttgtatactg gggccttata ttattcaata
     8581 tcataggat
//
LOCUS       DAOSNB010000079         8449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8346_length_8449_cov_4.129855, whole genome shotgun sequence.
ACCESSION   DAOSNB010000079 DAOSNB010000000
VERSION     DAOSNB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_8449_cov_4.129855"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLG42_04690"
     CDS             <1..483
                     /locus_tag="PLG42_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HOU30542.1"
                     /translation="WSLFTAPVSGVTYGANGIWPWLREGEKILNHSDAPWTYTWDKSI
                     NLPGSLQMKNIASFLKTLEWWNLYPASEILIEQPGDKQYNEFISVTKTPDNKTIVLYV
                     PVKTQVKIRKPLMRQYLVKWFNPVSGKYTDGVITDKGSVMEFSPPEETDMVAVLKIKK
                     "
     gene            complement(552..1781)
                     /locus_tag="PLG42_04695"
     CDS             complement(552..1781)
                     /locus_tag="PLG42_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOU30543.1"
                     /translation="MILFFLTSGLFLGWSLGANDASNVFGSAVGAKMVSFRKAAIIAS
                     VFVILGAVIQGAGTSGTLGKLGAVNAIGGSFTVALAAAITVYVMTKFSLPVSTTQAIV
                     GAIIGWNLFTGNSTDTSTLSKIVATWVTGPILGALFAIPLYILISRIKRRARIHMIQF
                     ESYIKTGLVVVGAFGAYSLGANNIANVMGVFVPAFDLSEMNLGILSLSSAQQLFLLGG
                     LAIAAGILTYSKKVMETVGSNIVNLTAEAALVTVLAQSLVLFVFSSTSLSNLLVKIGL
                     PPIPMVPVSSSQVIVGCVIGIGLYKGARNINFKILGEIAAGWVATPVIAGLTSFFSLF
                     IVRNIFNIDVGRKISESVETVQTPERAFDISQVMKYIILVIIIIGIVISLYYYMLERK
                     KNREFRKSEERFWKYIK"
     gene            complement(1793..2443)
                     /locus_tag="PLG42_04700"
     CDS             complement(1793..2443)
                     /locus_tag="PLG42_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOU30544.1"
                     /translation="MALFSRTTRQTIIKIDEFFDNIDLGILVFREGIRSYVKGDYESF
                     HRHLSKVDALENNADKLQREIENEMITHSILPQHREEVTRLIDEMDEIMDGIKSSLHE
                     FDIELPEIPESLFENIISLAETSANAAEELIPAARAYFRAPHTVREKLLKVYYFESET
                     DKISFSIKKRVFHEMKELDLAHKAHLRYIVHHIENISDLVQAAADMLSIMAVKVIL"
     gene            complement(2523..3893)
                     /locus_tag="PLG42_04705"
     CDS             complement(2523..3893)
                     /locus_tag="PLG42_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOU30545.1"
                     /translation="MYAIIDIETTGGSARLEKITEIAIYQHDGKKITGEYCTLVNPER
                     TIPYYITNLTGITNEMVEDAPKFYEIARNIVELTEGRIIVAHNARFDYSFLREEFKSL
                     GYNYRRKVIDTHSLSRKMFPGYRSYSLGNICSALGIKIENRHRAAGDALATVRLFELC
                     LEKDRELNGISSELLKNTRLAKLNPLLDIRKLEQIPEEPGIYYFYDEKGNVIYVGKSN
                     NIFERVSSHLSNNTSGRAMKMRDEIADISWELTGSELIALIRESIEIKSLQPLYNRAQ
                     RRLNNRWGICHGIDDKGYIVFSINRVKESRPFLATFNSKESVKTALERLIEKYSLCQK
                     LAGMYETEGACFHYQLGICRGACCGKESPQEYNRRASKAMDEFIFTPGNYFIIDRGRN
                     DNERSVVKIVRGRFAGYGYFDINCVGFGLEAIHECIKETDDNSEIISIIKSYLNHNRV
                     EKIVQF"
     gene            4076..4918
                     /locus_tag="PLG42_04710"
     CDS             4076..4918
                     /locus_tag="PLG42_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOU30546.1"
                     /translation="MSECFGNYFIHNGTLQPVELFDNSFMYEGESIYEVLRMIRGLPV
                     FFEDHFKRLETSTRHQKKLMLANREQLRKDILKLAEAEKIKDVNLKIVFNYNGVIGNY
                     LVYFIEPVYPTAEQYTKGVKGVLFYAERKNPESKVINHKLRSEIYHKLIICGAYEALL
                     VNRENCITEGSRSNIFFIKNNVLYTAPDRYVLGGITRKHIIEICGENDIKVEFVCVNA
                     DKIKEYDSVFMTGTSPVVLPFRTIENFSFNVKHPYIGFLRNLLLERMEKSLRYFENIS
                     DRGT"
     gene            5011..5310
                     /locus_tag="PLG42_04715"
     CDS             5011..5310
                     /locus_tag="PLG42_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30547.1"
                     /translation="MASIRELKKEIDNQIFGVISDCMLFMSRHPDHDENELNAIVQEA
                     YQLRNNLIYRIYHFDNKEDARAVKNHFKEIKSDLSNGVDQLCMKLSSLSSKKKKK"
     gene            complement(5291..6292)
                     /gene="amrS"
                     /locus_tag="PLG42_04720"
     CDS             complement(5291..6292)
                     /gene="amrS"
                     /locus_tag="PLG42_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOU30548.1"
                     /translation="MNFYKKADDRLICTLCPHNCKIAEGKTGICGVRKNTGGKIELLT
                     YGVISGFALDPVEKKPLYHFFPGMYILSIGSYGCNMRCDFCQNYHISQHVIAGISKKV
                     TPDEILSYSLKEKNNIGIAFTYNEPVIWFEYVKDVAVKVKEAGQSTVMVTNGYVTHEA
                     LKEYLQFIDAFNVDLKAFDNNFYKSLTGASLEPVKESIRMIAKAGKHLELTTLVIPGR
                     NDNPGDMEKEAKWIATELGRDVPLHLSRYFPMYKRNDPPTPSEVLIQLYEIASQYLDY
                     VYIGNAVQLTGQNTYCPQCGNLITKRDGYHIRHVGLLGKNCSKCGREIYRYVTSFSS"
     gene            6457..7713
                     /locus_tag="PLG42_04725"
     CDS             6457..7713
                     /locus_tag="PLG42_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU30549.1"
                     /translation="MTLKIKSINEAIAGIVIFFAILPELGGQGWKANPELVEKLSKSR
                     TDVNYYEEKVPTYILPELLVTLTGKEILKSSEWERYRRPEILELFTIHVYGRVPRTPY
                     NLDFKVVNEDPRAIGGLATAKQVDITVTAHDKSLVIHLYLFVPNSLKKPAPVFLLICN
                     RAPENIDPTRKNKSEFWPVEEAIARGYGMAAFYNADVDPDKYDGFKDGIHSILDVEPR
                     KDDSWGTLAAWAWGASRCLDYLVTDKQIDKNRIAVVGHSRGGKTALWAGATDRRFSMV
                     IANESGCGGSALARRRYGETIDIINKGFPHWFCLNYRKFNNNENSMPVDMHMLMALIA
                     PRALYVAAADEDLWGDPKGSYLSLYHSLPVYRLYNKKITLEKDVPPLNKQVISGNVGF
                     HIREGEHNLKLYDWNMFMDFADRVWK"
     gene            7704..>8449
                     /gene="yiaK"
                     /locus_tag="PLG42_04730"
     CDS             7704..>8449
                     /gene="yiaK"
                     /locus_tag="PLG42_04730"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF009750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HOU30550.1"
                     /translation="MEIIFIPADEMKSVFAEILLKNGFQNRKAETCADIFVQNSLDGV
                     ISHGVNRFPRFIQYIRDGYVKPDAEPTLISSSGATEQWDGNLGPGPLNALFATERAMK
                     LAEKNGIALVAMANTNHWMRGGTYGWNAAKKGFLFIGWTNTIANMPAWGAKDPRLGNN
                     PLVIAVPYGNEAIVLDFAMSLYSWGKIETCRLNGEKLPFPGGYDRQGRLTDDPSEIIM
                     TGRVLPAGYWKGSGLALLLDILATVISGGKP"
BASE COUNT         2596 a         1668 c         1632 g         2553 t
ORIGIN      
        1 tggagccttt tcacagcacc agtgtcaggt gtaacttacg gtgccaacgg tatatggccc
       61 tggctgagag agggtgagaa aatactgaac cacagcgatg ccccatggac ttatacatgg
      121 gacaaaagca ttaacctgcc tggttcgctt cagatgaaaa atattgcatc attcctcaaa
      181 acgcttgaat ggtggaacct ttatccggca tctgagatat tgatagagca accaggtgat
      241 aaacagtata atgaattcat atcagttacg aaaacgcctg ataataaaac tattgtgtta
      301 tatgttcctg tcaagacaca ggtaaaaatc cgtaaaccct taatgaggca gtatttggta
      361 aaatggttta atcctgtaag cgggaaatat actgacggag taattaccga taaaggatca
      421 gttatggaat tttctcctcc agaagaaact gatatggtag ctgttttgaa aataaagaaa
      481 taaaacaagc ttataagctc ctatatttaa ggctaatcat agttccaggc ttaaccttaa
      541 agtaaacctg attatttaat atacttccag aatctttctt cagactttct gaattctctg
      601 ttcttcttcc gctcaagcat ataataatat agagatataa caatcccaat aataattatt
      661 acaagaatga tatatttcat cacctgtgag atatcaaacg ctctttcagg agtctgaact
      721 gtttcaactg attcagagat cttccgtcca acatcaatgt taaaaatgtt tcttacaatg
      781 aaaagtgaaa agaacgatgt tagtcctgct attacaggtg tggcaaccca cccggcggca
      841 atctcgccaa ggattttgaa gttaatgttt ctggcacctt tatataaacc aattcctata
      901 acacagccaa cgattacctg agaacttgaa accggcacca ttggaatagg aggaagccca
      961 attttaacca gaagatttga aagagatgtt gatgagaaaa caaatagtac aagcgactgg
     1021 gccagaactg taacaagagc tgcttctgct gtgagattaa ctatgttgct tccaacagtt
     1081 tccattactt tttttgaata agtaagaatt cctgcggcta tagccagtcc tccaagtaaa
     1141 aaaagctgct gtgccgaaga gagacttaaa ataccaagat tcatttcact gaggtcaaaa
     1201 gcaggaacaa acacacccat aacatttgct atattgttgg ctcccagtga ataagcccca
     1261 aaagcaccga caacaaccag tcctgttttt atgtaagatt cgaattggat catatgaatc
     1321 cgggctcttc tttttatacg gcttatcagg atgtacagtg gtattgcaaa cagtgccccg
     1381 agtattggac ccgtgaccca tgtagcaact atttttgaaa gtgtactggt gtcggtggaa
     1441 tttcctgtaa acaggttcca tccaattatt gctcctacaa ttgcttgtgt agtagaaaca
     1501 ggaagcgaaa attttgtcat cacatacact gttattgctg ctgccagagc tacagtaaat
     1561 gaaccaccga ttgcattgac tgcgccaagt ttccccagtg tgcctgaagt gcctgctccc
     1621 tgtataactg ctcccagaat tacaaacaca gaagcaatta ttgctgcctt tctgaaggaa
     1681 accatctttg ctccaacagc tgaaccaaat acattggatg catcattcgc tcccagcgac
     1741 cagcctagaa ataaaccact cgttagaaaa aatagtatca taacagcctg tatcagagta
     1801 ttactttgac tgccattatc gacagcatgt cagctgctgc ctggacaagg tcagaaatat
     1861 tctcaatatg atgaacaata tacctcaggt gagccttgtg tgcaaggtca agctccttca
     1921 tttcgtgaaa aaccctcttt tttatactga atgagatttt atctgtctca ctttcaaaat
     1981 agtacacctt caggagtttt tctcttacag tatggggagc cctgaaataa gctctggcag
     2041 ccggaataag ttcttctgct gcatttgcgg aggtctctgc aagacttata atgttttcaa
     2101 acaggctttc aggtatttca ggtaattcaa tatcaaattc atggagggaa ctttttattc
     2161 cgtccataat ttcatccatt tcatcaataa gccttgtaac ttcttcccgg tgctgtggaa
     2221 gaatagaatg ggtgatcatt tcattctcta tttcgcgctg gagtttatcc gcattgtttt
     2281 caagagcatc tacctttgaa agatgcctgt gaaacgactc ataatcacct ttaacatacg
     2341 accttattcc ttcgcgaaaa acaagtattc caagatcaat gttgtcgaaa aactcatcga
     2401 tcttgattat tgtttgcctg gtggttcttg aaaataatgc catttttttt ctgattttca
     2461 gatttgtaaa caaaattaga aatctgacct gatttttcta atttatttat taacagctat
     2521 atttagaatt gaacaatttt ttcaactctg ttatggttga ggtagctttt tattattgaa
     2581 attatttcgc tgttatcgtc agtttcttta atgcattcat gtatagcctc caatccgaag
     2641 ccgacacagt tgatatcgaa atagccgtat cctgcaaacc ttccccttac aatctttaca
     2701 acacttcttt cattatcatt cctgcctctg tcaataatga aatagttccc gggggtgaaa
     2761 ataaattcat ccatagcctt tgatgctctc ctgttatatt cctggggtga ttcttttccg
     2821 cagcaggctc ccctgcaaat gcccagctgg taatgaaaac aggcaccttc tgtttcatac
     2881 atgcctgcaa gcttctggca taatgaatac ttttcaatga gtctctccag agccgttttt
     2941 acactttcct ttgagttgaa agttgcaaga aaaggtctgg attcttttac ccggtttatg
     3001 gagaaaacaa tatatccctt atcgtcaata ccgtgacaga tgccccatct gttgttcagt
     3061 ctcctttggg ccctgttgta tagtggctga agtgacttaa tttctattga ctctcgtatt
     3121 aatgcaatca attcacttcc ggtaagctcc cagctgatat cggcaatctc atcacgcatc
     3181 ttcattgccc ttcctgaagt attgtttgaa agatgagacg aaactctttc aaaaatatta
     3241 ttactcttac ctacgtatat gacatttcct ttttcatcat aaaaataata gatgccgggt
     3301 tcttccggaa tctgctccag ttttctaata tcaagcagag gattcagttt tgcaagtctt
     3361 gtatttttga gaagttcaga tgatattcca ttaagttctc tgtctttttc aaggcataat
     3421 tcaaacaggc gaactgttgc aagcgcatct cctgcagccc tgtgcctgtt ttcaattttg
     3481 attccaagtg cagaacatat atttccaagg ctgtaggatc tgtaaccggg aaacatctta
     3541 cgcgataatg aatgggtgtc tatcactttt cttctgtaat tgtaaccaag cgatttaaac
     3601 tcctcgcgaa gaaatgaata atcaaacctt gcattatgtg caacaattat ccttccttca
     3661 gttagctcaa caatatttct tgctatttca taaaacttag gtgcatcctc aaccatctca
     3721 tttgttatgc cggttaggtt cgtaatatag tagggtattg ttctttcagg atttaccaga
     3781 gtacagtact cacctgtgat ttttttaccg tcgtgctggt aaatggcaat ctcggttatt
     3841 ttttcgagtc tggcactgcc tcctgttgtt tctatgtcga ttatggcata cattaactga
     3901 ataaaggaaa gtcagagaag ttaaattaaa aagtttttat gaatctgaaa aatatgaaaa
     3961 gtaactgaaa ctttaacctc ataagaaggg ccgattttta aaagtattct ataattcagt
     4021 aattttcaat taataatatt agttttataa atttgtacgt ttaacatttt acaacatgag
     4081 tgaatgtttt ggtaattatt ttattcataa cggaacactt caacctgttg aattatttga
     4141 caattctttt atgtatgaag gagagtcaat atatgaggtt ctaagaatga tcagagggct
     4201 gcctgtgttc tttgaagatc attttaagcg tcttgaaaca agcaccagac atcagaaaaa
     4261 gttgatgctg gcaaacaggg agcagctcag aaaagacatt ctgaaactgg ctgaagcaga
     4321 aaaaattaaa gatgtcaatc ttaagattgt attcaattat aatggagtta taggcaatta
     4381 tctggtttat ttcatcgaac ctgtttatcc tacagccgag cagtatacaa aaggggtaaa
     4441 aggagtactt ttctatgctg aaagaaaaaa tcctgaatcc aaggtaatta atcataaact
     4501 tcgttctgaa atttatcata aacttattat ctgcggagct tatgaggctc ttcttgtaaa
     4561 tcgtgaaaac tgcataaccg agggaagccg ttcgaacatt tttttcataa aaaataatgt
     4621 cctgtataca gcccctgaca ggtatgttct tggagggata acacgcaaac atattatcga
     4681 aatatgtggc gaaaatgaca ttaaagtaga atttgtatgt gtgaatgctg ataaaattaa
     4741 agaatatgat agtgtgttta tgacaggtac ttctcctgtg gttttacctt tcaggacaat
     4801 agagaatttt tcctttaatg ttaaacaccc atatattggt tttctccgaa atctgcttct
     4861 tgaaaggatg gagaagagcc tcagatattt tgaaaatatt agcgaccggg gaacttaatt
     4921 ggggaataat cctcagggga atttttagtc tcacagacat aaataaattt ctttatcttt
     4981 gattctccaa tgttgtaaaa aataattgct atggcaagca tacgagagtt aaaaaaggag
     5041 attgataatc agatatttgg tgttatttct gattgcatgt tgtttatgag ccggcatcca
     5101 gatcacgatg aaaatgagtt gaatgcaatt gtccaggagg catatcagct tagaaacaac
     5161 cttatataca gaatatatca ttttgacaat aaggaagatg ccagggcagt taaaaaccat
     5221 ttcaaagaaa taaaatcaga cctttccaat ggtgttgatc agttatgcat gaagctcagc
     5281 agtctcagtt ctaagaagaa aaagaagtaa catatctgta aatttctctg ccgcattttg
     5341 aacaattttt tcccagtaag ccaacatgcc ttatgtggta accatctctt ttggtaatca
     5401 ggttaccgca ttgaggacag tatgtattct gaccggtcag ttgaacggca ttacctatat
     5461 acacataatc cagatattgt gaggcaattt catataactg tataagtact tccgatggtg
     5521 ttggagggtc atttcttttg tacattggaa agtaccggct aaggtgaagc ggaacatccc
     5581 ttcccagttc tgtggcaatc catttagcct ctttttccat atcacccggg ttgtcattcc
     5641 ttcccggaat gaccagagtg gttaactcca ggtgcttgcc tgcttttgca atcatcctga
     5701 tcgactcttt aaccggttca agtgaagcac ctgtaagtga tttgtaaaaa ttgttgtcga
     5761 aagccttgag atcaacatta aatgcatcaa taaattgcag atattctttc aatgcttcat
     5821 gtgtcacata accgttggtg accatcaccg tgctctgtcc ggcttcttta accttaacag
     5881 caacgtcttt aacatattcg aaccatatca caggttcatt atatgtgaac gcaataccta
     5941 tattattttt ttctttgagt gaataactaa gaatttcgtc gggagtaact ttctttgaga
     6001 ttcccgctat gacatgttgc gatatatggt aattctgaca gaaatcacat cgcatgttgc
     6061 atccatacga acctattgaa agaatataca ttccggggaa aaagtgatac aacggcttct
     6121 tttcaacagg gtctagcgca aagcctgaaa taaccccgta tgtaaggagt tcaattttac
     6181 ctccggtatt tttccttact ccgcatatgc ctgttttgcc ctctgcaatt ttacagttgt
     6241 gggggcaaag tgtgcatata aggcggtcat ctgccttttt gtaaaaattc atgaaatgaa
     6301 taaatttatt acaaagataa agagatacaa tttttcattt gtgattaaaa taagattttc
     6361 agcattttta tggagaacac tatattttta aagttaaatt cggtttaaga aaattctgaa
     6421 gaatttgtat tttcacctga aattataaag attataatga ccttgaaaat aaaaagcatt
     6481 aatgaagcaa ttgcgggtat tgtcattttt ttcgcaatac tacccgaatt gggcggacag
     6541 ggatggaaag caaacccgga gctggttgaa aaactctcaa aaagcagaac agacgttaat
     6601 tattatgaag aaaaagttcc aacctatatt ctgcccgaac tgcttgttac cctgacagga
     6661 aaagaaattc tcaaaagttc ggagtgggag agatacaggc gaccggaaat actcgaactt
     6721 tttacaatac atgtctacgg cagggtccct cgaacacctt acaaccttga tttcaaggtt
     6781 gtaaacgaag accccagagc aataggagga ctggcaactg ccaaacaggt agatataacc
     6841 gtaactgctc atgataaatc ccttgtgata catctatatc tgttcgttcc gaatagcctc
     6901 aaaaagccgg cacctgtttt tctgctgata tgtaaccggg cacccgaaaa tattgacccc
     6961 acacgcaaaa ataagagcga gttctggccc gttgaagagg ccatagcaag aggttacggc
     7021 atggcagcct tttataatgc cgacgttgac cctgataaat acgatggatt caaggacggc
     7081 atacactcaa tcctcgatgt tgaaccccgc aaagatgatt cgtggggaac ccttgctgcc
     7141 tgggcatggg gagccagcag atgtctcgat taccttgtta cagataaaca gattgacaaa
     7201 aaccggattg ctgttgtagg acattcacgt ggtggaaaga ctgccctctg ggccggcgca
     7261 accgacagga gattctctat ggtaattgca aatgaatcag gatgcggagg ctcagcactt
     7321 gcaagaagac gatatggaga aaccattgat ataatcaata aaggatttcc acactggttt
     7381 tgcctcaact acagaaagtt caataataat gagaattcaa tgcccgttga tatgcatatg
     7441 ttaatggcat taattgcacc gagggcactc tatgtagctg ctgccgacga agacctctgg
     7501 ggcgacccaa aaggctccta cctctcactt tatcactcct tgcctgtata ccggctttat
     7561 aacaagaaaa taaccctcga aaaagatgta cctcctctta ataaacaggt aataagcggg
     7621 aatgtcggtt ttcatatacg cgaaggagaa cataatctta aactttacga ctggaacatg
     7681 tttatggatt ttgccgacag ggtatggaaa taatatttat tcctgctgat gaaatgaaat
     7741 ccgtttttgc agaaattctg ttaaaaaacg gttttcagaa cagaaaagct gaaacatgcg
     7801 ccgatatatt cgttcaaaac agcctcgacg gagtcatctc acatggagta aatcggttcc
     7861 cccgtttcat tcaatatata agagatggat atgtaaaacc agatgcagaa ccaacactga
     7921 tatcatcttc aggtgccact gaacaatggg acggaaacct tggacctggc cccttgaatg
     7981 cattatttgc aaccgaaagg gcaatgaaac tggcagagaa aaacggaata gcccttgttg
     8041 ccatggcaaa tactaaccac tggatgaggg gtggtaccta tggctggaat gctgcaaaaa
     8101 aaggttttct attcattgga tggacaaata ctatagccaa tatgccggca tggggtgcaa
     8161 aagacccccg actcggtaat aatccgcttg ttatagctgt tccatatggt aatgaagcta
     8221 ttgtactcga ttttgccatg tcgttatatt cctgggggaa aatagaaaca tgcagactta
     8281 acggtgaaaa actgcctttt cccggaggct atgacagaca aggtagactg acggatgacc
     8341 catcagaaat aatcatgact ggaagggtgc tgcctgctgg atactggaaa ggctcaggac
     8401 ttgccctcct gctggatatc ctcgcaacag taatttccgg tggtaagcc
//
LOCUS       DAOSNB010000080         8439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8359_length_8439_cov_5.039480, whole genome shotgun sequence.
ACCESSION   DAOSNB010000080 DAOSNB010000000
VERSION     DAOSNB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_8439_cov_5.039480"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLG42_04735"
     CDS             <1..415
                     /locus_tag="PLG42_04735"
                     /inference="COORDINATES: protein motif:HMM:NF020752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOU30551.1"
                     /translation="RLEPSTDSTVNVDEIKAKCYEALDDDLNSPVLLAQLFEAVKLIN
                     SAREGTEKMTASDIAKMKELFSVFVKDILGLIDESAGRGNEKIVSGLMKLILDIRHEA
                     RQKKDFATSDKIREELSRLGITVRDLKDGAEWEFE"
     gene            428..2290
                     /gene="ispG"
                     /locus_tag="PLG42_04740"
     CDS             428..2290
                     /gene="ispG"
                     /locus_tag="PLG42_04740"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOU30552.1"
                     /translation="MDNEVKPYRSSVVFVGSVPLGGDYPIRIQSMTNTDTLDTEASVA
                     QCIRIIEAGADFVRLAAQGTKEARNLENIKKELLKAGYNTPLIADIHFNPAAAEVAAE
                     IVEKVRINPGNYADRRSSSGKILSDSEYEEELERIHTRLLPLINICHRNNTAIRIGVN
                     HGSLSARIIERYGNTPQGMVYSAIEFIKIFRAENFHRLVISMKSSDTSVMIEANRLLV
                     KMMQQEGYYYPLHLGVTEAGEGEDGRVRSAAGIGTLLAEGIGDTIRVSLTEEPEKEIP
                     VAKMLAGFLPRGVSDSRVKYTPAFRTAPLRPSPLRKVNGPIVMLNMTRYEKLNIASFR
                     EAGFTVDESKKEITGGDNTPDLIIASQVPEIIPSSTCVAVPFKIWMKNRSRENLYPVF
                     SPDTWPGRESASPDLNVIFTEPRTDISLLSKIDPSTAVLVFTYRVSVNMFAPDEFLRS
                     LSSRKLNFSIILNPVFENESIEELRIKAPAVLGGYLNDRNVSGISITSLAVANSGIIN
                     SLCFSILQCTGTRITRNIYISCPTCGRTKFNLQEVLRKVKEKTSHLRGLKIAVMGCVV
                     NGPGEMAGADWGYVGASEGKVHIYKGTEPVLKNVSEDKAIDELLRLIEEDQASR"
     gene            complement(2275..4404)
                     /gene="rnr"
                     /locus_tag="PLG42_04745"
     CDS             complement(2275..4404)
                     /gene="rnr"
                     /locus_tag="PLG42_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOU30553.1"
                     /translation="MAKRKKGEGKKSLSSAELEELVKGVLNNYAGQSFNYKQIAKKLN
                     ITDEHQRKRIIGILSSLASKGLIKETRRGRYLVKAGKGYITGTVELTRMGYGFVISDE
                     IDDDVFVSSRNLKTALHGDKVKVRLYARRKGTRPEGEVVAILERAKSTFVGTVEIVSN
                     MAFLIPDNKNMPFDIFIPKSKINGAKQGQKAVARVIEWDSRAKNPVGEIVEVLGYPGE
                     HETEIHAILAEFELPYDFPPEVEKEAEKIPDAITGEDLKGRRDFRDVPTFTIDPADAR
                     DFDDAISLRRLDNGNWEVGVHIADVTYYVKPGTLLDQEARQRATSVYLVDRVVPMLPE
                     KLSTHVCSLNPYEDKLTFSVVMEMNDNAEVLSEWFGRTVIRSDRRFSYGEAQKIIDTG
                     EGDMKEQLLVLNRLAQILRKNRFAAGSFFFERVEVQFDLDEHGKPLAIKFRDMGTANQ
                     LIEEFMLLANRRVAEFVGKKLKGKTFVYRIHDKPDPEKLDSFVHFIQRFGYKLEVNNI
                     NNLPKAMNKLLNQAEGTNEQNIIETLAIRSMAKAVYSTDNIGHYGLSFRYYTHFTSPI
                     RRYPDIMVHRLLAMYLNKENPGGKEKYEKLCEHSSKMERVAMEAERASIKYKQVEFMA
                     DKIGKVFEGVISGVTEWGIYVEIIENQCEGMIHIRDLDDDYYEYDESNYCIRGRYTKK
                     EYRLGDRVKVEVVKADIQKKQLDYRLA"
     gene            4476..4763
                     /locus_tag="PLG42_04750"
     CDS             4476..4763
                     /locus_tag="PLG42_04750"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOU30554.1"
                     /translation="MELLNQIPSRSSSVVTRKTGNEYIIVPVTDNIADMDSIFTLNET
                     GAFIWEQIDGEKSVEEIIRLVVDEFEIDRDTATKDVLGFLSTMKEYLIIKM"
     gene            4811..5722
                     /locus_tag="PLG42_04755"
     CDS             4811..5722
                     /locus_tag="PLG42_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30555.1"
                     /translation="MREYCLNIAGYNIKVKSAPGGPLLGPSARFEGSRVQSNKHDILI
                     RVHTGQFILPAGAERVFHAPFVGEREGKTFIRSEEFWDVYRDKEGRLYICTIFPLEES
                     QDRGTLIFSLRSDVWELWIEGKGRKIDPLAYPLDGLLLYYLTVISNDIMIHASGVSFE
                     DSGFIFSGVSGKGKSTIARLWHEAGARVIHDDRLIIRRMNGEYRFYNTPVYNNDKPLE
                     APLSAIFLIEHGMENRIEPLSGVQAVSQVLANCIQHNWDKNITGNLVNAITDMCEKIR
                     VFRFYFKPEPSAINFILDHEHSQKTYR"
     gene            5694..6155
                     /locus_tag="PLG42_04760"
     CDS             5694..6155
                     /locus_tag="PLG42_04760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOU30556.1"
                     /translation="MSIHRKHIDELRNMSVSLLAEGKTIRIKAHGYSMYPTIKPGMVI
                     MIEPIRLKGLPQKGEIVAINKDKGIVVHRIVEIIDEGGKKKYIARGDSNPFPDAPVTI
                     DMIPGRVTGLEGFARLPAEFSKKPNYFLNRLRVIMIHIAGRFKNLKNKIIN"
     gene            6167..7966
                     /locus_tag="PLG42_04765"
     CDS             6167..7966
                     /locus_tag="PLG42_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU30557.1"
                     /translation="MKNLRLLFESVKLVRESAPGWTAANVAVSLLRSFIPLVLLFLIR
                     SLIDTITQGVSSGGEVSTARIVYSITAVIVVFLIDEMSSEIGNYIRKKQSYRLEEYMY
                     KLLHSKATALDLINFENPSYFDKLSRAVREATWRPNNILNNFVALFRGIISLILMASL
                     LFTLNWWIALLLVVVNIPGIWMRLHYASVLYYFRKELTPEERKAGYFNWLLTGDRPSR
                     EIRLFGLGDYFISHFKESFRQYKEKEIDILRKRTAIETVSGVIKASALFIVLYVIARQ
                     TISGEITLGNMAMLILAFRQGLIYIRDILSSVAGLYEDSLFTGDIFEFLMLKEKIKEE
                     EPVSEVTQLQKEIRFENVTFTYPGNNSPALKDVNLTLKKGEIVALVGSNGSGKSTLVR
                     ILCRLYDPDSGAVFFDGKNIRNFKPEEYRKQFSVLFQDFMLYNLSAGENILLGNIEAG
                     RQEERMKLAAMSAGIHSLIESLPGGYDTQIGPLFDESRELSWGEWQKIALARTIYRDA
                     SVMILDEPSSAIDSESEYEIFSRFREIVKGQTVLLISHRFTSVRLADRVVVLEGGRIA
                     ENGTHTELMMKKGLYYNMYTRQKNMLGDGEQTS"
     gene            7947..>8439
                     /locus_tag="PLG42_04770"
     CDS             7947..>8439
                     /locus_tag="PLG42_04770"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOU30558.1"
                     /translation="MGNRLLEIRDEEILLIGLCRLSFDEKLRGRISELVRLTSDWDYF
                     TNLAMEHGIAALVWHNLEKLELLNYLPEKNASVLHSSLLLSVTRNAFHLTGAEEVISL
                     LNRAGIKVVLLKGLALELTLYGNSGLRQMTDIDLLISRRDFLKARDILLHNGYDSLPV
                     KSGF"
BASE COUNT         2431 a         1615 c         1954 g         2439 t
ORIGIN      
        1 caggcttgaa ccttcaacgg attccacagt aaatgttgat gaaataaaag caaagtgcta
       61 tgaggcactt gatgacgacc tgaacagtcc tgtccttctg gcacaactat ttgaagcggt
      121 aaagctgata aactcagcca gagaaggcac agaaaaaatg actgcctccg acattgcaaa
      181 gatgaaagag ctcttttcgg tttttgtaaa agatatttta ggactcattg atgagtcagc
      241 aggcagaggt aatgagaaga ttgtaagcgg gttgatgaaa ctcattcttg atataaggca
      301 tgaggcaagg cagaagaagg attttgcaac atctgacaaa ataagggagg aactgagccg
      361 gctggggata acggtgaggg atttgaaaga tggtgcggaa tgggagtttg aataaagaca
      421 ggtcaggatg gataatgagg tgaagccata ccgttcttct gtagtttttg taggttctgt
      481 tccgcttggg ggagattatc ccatccgaat acagtcgatg acaaataccg ataccctcga
      541 cactgaggct tcggttgccc aatgtataag aataatagaa gcgggggctg attttgtaag
      601 gctcgccgca cagggaacaa aagaagcccg gaatcttgaa aatatcaaaa aggagctctt
      661 aaaagccggc tataacacac cgcttattgc agatattcat ttcaatccgg ctgctgctga
      721 ggtggctgca gaaattgtcg aaaaagtgag aattaatcct ggtaattatg ctgacaggcg
      781 ttcatcctca ggtaaaattc tttcggactc agaatacgaa gaagagcttg aaagaataca
      841 tacaagactg cttccactga taaatatatg tcacagaaac aacaccgcta ttcgtattgg
      901 agttaatcat ggttcactat cggctagaat aattgagcgg tatggaaata caccgcaggg
      961 aatggtttat tcggcaattg aattcataaa gattttcagg gccgagaatt ttcaccggct
     1021 ggtaatttcg atgaaatcgt ccgacacatc tgtaatgatt gaagccaata ggttgctggt
     1081 aaagatgatg cagcaggaag gatattatta tcctctgcat ctgggggtta cggaagctgg
     1141 tgagggggaa gacggcaggg tacggtctgc ggctggcatc ggaacactcc ttgctgaagg
     1201 aataggggat acaataaggg tttcccttac tgaggagccc gagaaagaga tccccgttgc
     1261 aaagatgctt gccgggtttc ttccccgggg tgtttcagac tccagggtta agtatacgcc
     1321 tgccttcaga actgcgccac tgcggccatc tccactgcga aaagtcaacg gccccatagt
     1381 gatgctaaat atgacccgtt atgaaaaact taatatagcc tcattcaggg aagcaggatt
     1441 cactgttgat gaatcaaaaa aagaaataac tggaggggac aatacacctg atctgattat
     1501 cgcttcccag gtacctgaaa taataccttc ttcaacatgt gtggcagttc cgtttaaaat
     1561 atggatgaaa aacagaagca gggaaaacct ttatcctgtt ttttctcccg acacatggcc
     1621 aggcagggag tcagcctcac ccgatctgaa tgtgattttt acagagcccc gtacagatat
     1681 ttcacttctt tcaaaaattg atccctcgac ggcagttctt gtttttacgt atagagtttc
     1741 ggtgaacatg tttgctcctg atgaattttt aaggagttta tccagcagaa aacttaattt
     1801 cagcataatt ttgaatccgg tttttgaaaa tgaatctatt gaagaattac gtataaaagc
     1861 accagcagtg cttggcggtt atttgaatga caggaatgta tccggtattt caattacctc
     1921 actggcagtt gctaattcag gcataattaa ttcgctgtgc ttttccattc ttcaatgcac
     1981 aggtacaaga attacacgaa acatatatat ttcctgtccc acctgcggga ggacaaagtt
     2041 caacctgcag gaggttctta gaaaagttaa agaaaagact tctcatctcc gggggctgaa
     2101 aattgctgtg atgggttgtg tggtaaacgg tccaggtgag atggctggtg ccgattgggg
     2161 atatgtggga gcctcggaag ggaaagttca catatataaa ggtacggagc cggttttgaa
     2221 aaatgtcagt gaagacaaag ctattgatga acttttaagg ctaattgaag aagatcaggc
     2281 aagccggtaa tcaagctgtt ttttctggat atcggccttt acaacttcaa cctttaccct
     2341 gtcgccaagc ctgtattcct tttttgtata tctgcccctt atacaataat tgctttcgtc
     2401 gtattcgtag taatcatcat cgagatcgcg gatatgaatc atgccttcac attgattctc
     2461 aataatttca acgtagatgc cccattccgt tactccagaa atgacacctt cgaagacttt
     2521 acctatctta tccgccataa actctacctg tttgtattta attgaggctc tttctgcttc
     2581 cattgcgaca cgttccattt tagaggaatg ttcacacagt ttttcgtatt tttcttttcc
     2641 tccgggattt tccttattga ggtacattgc aaggaggcgg tgaaccataa tatcagggta
     2701 gcgtcttata ggggaggtga agtgggtata atacctgaaa gataggccgt aatgccctat
     2761 attatcggta gaatagacag ctttggccat tgaacgtatt gcaagggttt caattatgtt
     2821 ctgttcgttt gttccctctg cctgattgag cagtttgttc atggctttcg gaaggttatt
     2881 gatattattt acttcgagtt tatatccgaa tcgctggatg aaatggacga atgaatcgag
     2941 tttttccgga tcaggtttgt cgtgaatacg gtaaacaaat gttttccctt ttaatttttt
     3001 acccacgaac tcagcaaccc ttctgttggc aaggagcata aattcttcta tcagctggtt
     3061 ggcagttccc atatccctga actttattgc aaggggttta ccatgttcat cgaggtcgaa
     3121 ctgaacctca acgcgttcaa agaagaatga accggcggca aaacggtttt tcctcaggat
     3181 ctgggcaagg cggttgagta caaggagctg ctctttcata tcaccttctc cggtgtcgat
     3241 aatcttctgg gcttctccgt atgagaacct ccggtcggat cttatgaccg tccttccaaa
     3301 ccattccgac agaacttctg cattatcgtt catctccatt acaactgaga aagtcagttt
     3361 atcttcatac ggattaagag aacatacatg ggttgataac ttttcaggga gcatcggaac
     3421 gactctgtcg acaagatata ccgatgttgc tctctgccgg gcttcctggt cgaggagtgt
     3481 accaggtttt acatagtatg ttacgtcggc aatatgtaca cccacttccc agttgccgtt
     3541 atcaagcctg cgcagggata tagcatcatc aaaatcgcgt gcatctgcag gatctatcgt
     3601 aaaagttggg acatccctga agtctcgacg ccctttaaga tcttccccgg taatggcatc
     3661 aggaattttc tctgcttctt tttcaacttc cggcgggaaa tcataaggga gttcgaactc
     3721 ggcaagtatt gcgtgtattt cagtttcatg ttcaccagga taaccaagca cttcaactat
     3781 ctcgccaacc ggatttttag ccctcgaatc ccattctata acccttgcaa ccgctttctg
     3841 cccctgtttg gcaccattga tttttgattt cgggatgaaa atgtcaaagg gcatattctt
     3901 attatcggga ataaggaaag ccatgttgga aactatctct acagttccga caaatgttga
     3961 ttttgccctt tcaagtatgg caactacctc tccttcgggt cttgttcctt ttctccgggc
     4021 atatagtcta actttcactt tgtcgccatg aagagctgtc ttcagatttc tggatgaaac
     4081 aaacacatca tcatctattt cgtcggaaat aacaaagccg tatcccattc ttgtaagctc
     4141 tacagtaccg gtaatatatc ctttgccggc tttcaccagg taccggccac ggcgtgtttc
     4201 tttaataagt ccctttgaag ccagggatga taaaatgcca attattcgtt ttcgttgatg
     4261 ctcatcggtt atattcagct ttttagctat ctgcttgtaa ttgaatgatt gtcctgcgta
     4321 attgttcaga acacctttta caagttcctc aagctcagca gaggagagag attttttccc
     4381 ttcgcctttt ttacgtttag ccatttttat ggtttttgca aaggtaagaa ttaccaaagg
     4441 ttaaaattca taaatttgac atatattgaa attttatgga attacttaat cagatacctt
     4501 cccgttcttc atcggttgtg acacgcaaga ctggaaatga atatattatt gtcccggtaa
     4561 cagacaatat tgcagatatg gacagtatat tcacccttaa tgaaacaggg gctttcatat
     4621 gggaacagat tgacggcgaa aaaagtgttg aagagattat ccggcttgtc gtggacgaat
     4681 ttgaaatcga cagagatact gcaactaaag atgtactggg ttttttgagc acgatgaaag
     4741 aatatctgat catcaaaatg tgagaattta gatttcttcc cggattctta tgtgacacaa
     4801 taaattgtaa atgagagaat actgcctgaa tattgcagga tataatataa aggtaaaatc
     4861 tgcacctggc ggacccctcc ttgggccttc ggcaagattt gaaggaagtc gggtgcagag
     4921 taataaacat gatattttga taagagttca caccggacaa tttattctgc ctgctggagc
     4981 agaaagggtt tttcatgctc cttttgttgg ggagagagag ggaaaaacct ttataaggag
     5041 tgaggagttc tgggatgttt accgtgataa agagggcagg ctctacatct gtaccatatt
     5101 cccgcttgaa gagtcgcagg atagaggtac tcttattttt tcgctgcggt cggatgtctg
     5161 ggagctctgg attgaaggta agggaaggaa gattgatccg ctggcttatc cgctcgacgg
     5221 acttctgctt tattatctta ctgtaataag caacgatatc atgattcatg cttcaggagt
     5281 cagttttgaa gatagtggct ttattttcag cggggtgtcg gggaaaggaa aatcaacaat
     5341 tgcacgtttg tggcatgaag ccggcgccag ggtgatccac gacgacagac ttataatcag
     5401 aaggatgaat ggtgagtacc gtttctataa tactcctgta tataataatg acaaacctct
     5461 tgaagcaccg ctcagcgcaa tatttctaat tgagcacggt atggaaaata gaattgagcc
     5521 attgagcggt gtacaggcag tgagccaggt gcttgcaaac tgtattcagc acaactggga
     5581 caagaatata actggtaatc ttgttaacgc tataacagat atgtgcgaaa aaatcagagt
     5641 tttccggttt tatttcaaac ctgaaccttc agcaattaat tttatactgg accatgagca
     5701 ttcacagaaa acatatagat gaattaagga acatgagcgt ttcgcttctt gcggagggga
     5761 agacaataag gatcaaagct cacggataca gcatgtatcc caccataaaa cctggaatgg
     5821 taatcatgat tgaacctatc agattaaaag gtctgcctca gaagggcgaa attgttgcaa
     5881 taaataagga caagggaata gtggtgcacc ggattgttga aattattgac gagggaggaa
     5941 aaaagaaata tattgcacgt ggcgacagca atcctttccc tgatgcacct gtaacaatcg
     6001 atatgatacc cggcagagtc accgggttgg aaggttttgc ccggttgcct gctgaatttt
     6061 caaagaagcc aaattatttc cttaaccgtt tgagagtgat tatgatacat attgccggca
     6121 gattcaagaa tctgaaaaat aaaataataa actgacatct ggcctgatga aaaatttaag
     6181 acttcttttt gagtcggtaa agcttgtgag ggagagtgct ccggggtgga ctgctgcaaa
     6241 tgttgcagtc tctttgctca gaagttttat tccacttgta ctgctgttcc tcataagaag
     6301 cctgattgat acgataaccc agggtgtttc atcaggcgga gaggtatcta ctgcccgaat
     6361 tgtctattca attacagctg ttatagttgt atttttgatt gatgagatgt cgtcagaaat
     6421 agggaattat ataaggaaaa agcagtcgta ccgtcttgag gaatacatgt ataagctttt
     6481 gcattcaaaa gccacagctc tggatctgat taatttcgaa aatccttctt attttgataa
     6541 attgtcgagg gcagtcagag aagctacatg gcgaccgaat aacatactca acaacttcgt
     6601 tgctctgttc agggggatta tttcacttat tctaatggcc agccttctct ttactttgaa
     6661 ctggtggata gccctgctgc ttgtagtagt aaatattcca ggcatatgga tgaggctaca
     6721 ttatgcttca gttctttatt actttagaaa ggaactaacg cccgaggaga ggaaagcagg
     6781 atatttcaac tggttgctaa caggagaccg gccatcgagg gaaataaggc tatttggact
     6841 cggtgattat tttatctctc atttcaaaga gtcgttcaga caatataaag aaaaagaaat
     6901 agacatttta cgcaagagaa ctgctatcga aacagtttca ggcgtaatta aggcatcagc
     6961 actttttatc gtgctttatg ttattgcacg tcagacgata agcggggaaa ttacgctcgg
     7021 aaatatggca atgctcatac tggccttcag acaggggttg atatatataa gagatatcct
     7081 gagttcagta gcgggtcttt atgaagattc attgtttacc ggagacatct ttgaattttt
     7141 gatgctgaaa gaaaaaataa aagaagaaga gccggtttct gaagtaactc agcttcaaaa
     7201 ggaaataaga tttgaaaatg taacttttac atatcccggt aataattctc cggcgctgaa
     7261 agatgtcaat ctgacactaa agaaggggga gattgttgcg cttgttggtt cgaatggctc
     7321 cggtaaaagt actctggtgc gaatactttg cagactctac gaccctgata gtggggctgt
     7381 attttttgac ggaaaaaata taaggaattt taagccggag gaatacagaa aacagttttc
     7441 ggtacttttt caggatttca tgctgtacaa tttgagcgcg ggggagaaca ttttacttgg
     7501 aaacatagaa gcagggaggc aggaggaaag gatgaagctt gctgcaatgt cggcaggtat
     7561 acactcattg attgaatcac tgcccggagg ttatgataca cagattggac ctctgtttga
     7621 cgagagccgt gaattgagct ggggtgaatg gcagaagatt gctctggccc ggaccattta
     7681 tcgtgatgca tcagtgatga tactcgatga gccttcgagt gctattgatt cggaatcgga
     7741 atatgagatt ttcagccgtt tcagagaaat agtcaaaggg cagaccgtgc tgcttataag
     7801 tcaccgcttc accagtgtca ggctagccga cagggtagtt gtcctggaag gtggcagaat
     7861 agcagaaaat ggaacacaca ctgaattgat gatgaaaaag ggattgtatt ataatatgta
     7921 tacaaggcag aaaaatatgc taggggatgg ggaacagact tcttgaaata agggatgagg
     7981 aaatattgct cataggtctt tgccgccttt catttgatga gaaacttcgt ggcagaatta
     8041 gtgaacttgt tagattaacg agtgactggg attatttcac gaatcttgct atggagcatg
     8101 gaatagctgc acttgtgtgg cacaatcttg aaaagcttga actgttgaac tatcttcctg
     8161 aaaagaatgc ctctgtgctc cattcttcac ttctgttaag cgttaccaga aatgcctttc
     8221 atctgactgg agctgaagaa gtaatatctt tgcttaacag ggcaggtata aaagttgttc
     8281 ttcttaaagg tcttgccctt gaactgactt tatacggaaa ctcaggcctg cgccagatga
     8341 ctgatataga cctgctgata agcaggcgcg atttcctgaa agcaagagat attcttctgc
     8401 ataacggcta tgattcgttg cctgtaaaat cgggttttc
//
LOCUS       DAOSNB010000081         8370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8446_length_8370_cov_4.860493, whole genome shotgun sequence.
ACCESSION   DAOSNB010000081 DAOSNB010000000
VERSION     DAOSNB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8446_length_8370_cov_4.860493"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="gcvPA"
                     /locus_tag="PLG42_04775"
     CDS             complement(<1..1152)
                     /gene="gcvPA"
                     /locus_tag="PLG42_04775"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOU30559.1"
                     /translation="MTSDKFVKRHIGPRDNELDGMIATIGVSSLDELIDKTIPSSIRL
                     TKPLNLPPAMSEHEYLNHIRKLGAMNKQYRSFIGQGYYGVAVPSVIIRNVLENPSWYT
                     SYTPYQAEISQGRLEALLNFQTMIIELTGMQIANASLLDESTAAAEAMIMMFNARPKE
                     AVRAGAVKFFVDNDIFLQTLDVLKSRSAPLGIELVTGNYEEFTPDNTFFGAIVQYPSA
                     SGMIRDYRDFALKIHSAGALLCVAADLMSLALLTPPGEWGADVVVGSSQRFGLPMSYG
                     GPHAGYFATKEELKRNMPGRIIGISVDAQGNPALRMALQTREQHIKRERATSNICTAQ
                     ALLAIMSGMYAQYHGPEGLKSIAHHIHRTACTLSDALKEAGFTNSNSQFF"
     gene            complement(1253..1888)
                     /locus_tag="PLG42_04780"
     CDS             complement(1253..1888)
                     /locus_tag="PLG42_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU30560.1"
                     /translation="MEVFRLVFSPIEVNTYIVADTTGECAVIDCGCYNEKEFQRLVRF
                     IELKNFKPVLLLNTHCHLDHVFGNKFMFEKYNLRTYCHREEMPNLKNAPAHAEFFGLK
                     MDEPPEPEAFIEDGEIIEFGNISLKTMHVPGHTAGGVAFYCEKESCIFTGDTIFAGSI
                     GRTDLPGGNQNTLLKSIKEKILILNPSTIIYSGHGPATTIENELKRNPWLI"
     gene            complement(1900..2550)
                     /locus_tag="PLG42_04785"
     CDS             complement(1900..2550)
                     /locus_tag="PLG42_04785"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOU30561.1"
                     /translation="MQDTFEARGLREKLVESLREKGITDEEVLRAMRTVPRHLFMDPA
                     FLHHAYADKAFPIAAGQTISQPYTVAIMTSLLKVKKRDKILEIGTGSGYQAAILAEMG
                     ARVYTIERCRELYLKAQQTIAELGYHNIHFFFGDGWEGKKEYGPYDGIIITAAAPEIP
                     DKLLQQLKTGGRLVVPLGGGDYQIMTVVERTGPDSYDYSSHGTFVFVPMLRGTVNG"
     assembly_gap    3095..3104
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3105..4091
                     /locus_tag="PLG42_04790"
     CDS             <3105..4091
                     /locus_tag="PLG42_04790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOU30562.1"
                     /translation="HRGLVGYKTLILGSKKKALEIYRRIREEAIPGGNILTGYIIING
                     GANHKSEWDLECLGNAEDILKVINEYQIEEVILAVEDDEFEKLLDIIGQLQFTDVTIK
                     AIPSLKDLLTGRVEHTSIFGTPLLEIPNKTMTHTQAIIKQIIDYAASIILLILLIPVI
                     LALAIAIKLYDGGPILYRQERIGRNGKPFTILKFRSMKKDAESTGPMLSGKDDPRITP
                     LGRFMRKHRLDEIPNLLNVLKGDMSLVGPRPERQYYIEQIVKKAPHFRRLLKVKPGIT
                     SWGQVKYGYASNIDQMVERLEYDLIYLENMNLLVDLKIIIYTVMIILKGKGM"
     gene            complement(4124..4678)
                     /gene="thpR"
                     /locus_tag="PLG42_04795"
     CDS             complement(4124..4678)
                     /gene="thpR"
                     /locus_tag="PLG42_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOU30563.1"
                     /translation="MRRTFVAVKAEPGEKLRKAIALLRNGLNRDSIKWVDPAIMHLTL
                     AFLGDTEEENIELMSRNLQEKCSGSGEISFTIKGLGVFPGISNPRVIWAGIENAETLL
                     NLQKIVSGIMTSLNIPAEEREFKPHLTLGRVKFLRNNEKLKEIISEHENVEFQNVKLN
                     KLIYYESVLMPGGPLYKPITEVIL"
     gene            4817..5569
                     /locus_tag="PLG42_04800"
     CDS             4817..5569
                     /locus_tag="PLG42_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOU30564.1"
                     /translation="MYINLYQLSLFLIFCFAVLVFILLFYIPAPYGKFMRRGWGPSVK
                     SGLAWMIMEFPSPFLMAYFFLTSTHKNIVHYVFILMWLAHYLYRTFIYPFMQSGRKKP
                     YPVVVVLMAVIFNVLNGFVNGYGVFHLYSYDKSWLFSWQFIAGFVLFASGFIINKTAD
                     SKMMHLRNNNSGEYVIPRGWLFEYISSPHYFGEIVEWCGWAIMTFSLPGLAFAVFTFA
                     NLFPRAIASHKWYRQHFSDYPSGRKAVIPFII"
     gene            5666..6466
                     /locus_tag="PLG42_04805"
     CDS             5666..6466
                     /locus_tag="PLG42_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOU30565.1"
                     /translation="MMIIEELINTDTYKSPVPEPGFRNWLLRDNWFYFIINFGRIVLR
                     TRREALEGRYDYPEWIYSSHEILRLLEKIGGRFDIEGMNNISRISEPVIFVSNHMSTL
                     ETMIFPGIIAPRRKVTFVVKESLVRNYFFRDVMRSRDPIVVGRTDPRRDFETVMSQGP
                     LLLEKGISIIVFPQSTRSREFIPSEFNTMGVKLAKKTNKYVVPVAIKTDFWGNGKIIK
                     EFGKFDHRKTIHIKFGEPLNVKGTGKEENQAIIEFIQLNLEKWEKEHN"
     gene            6655..8307
                     /locus_tag="PLG42_04810"
     CDS             6655..8307
                     /locus_tag="PLG42_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU30566.1"
                     /translation="MATLDWIVLILFCLGLVGIIVWVVKQKTETSADYFLAGRDATWL
                     AIGASIFASNIGSEHLVGLAGAGASSGMAMAHWEMHAWVILILGWVFVPFYSRSKVFT
                     MPEFLEKRYNRGARSFLSVISLVSYVLTKVAVTVYAGGIVFKQVFGIESMFGIDFFWI
                     SAVGLVILTGIYTVLGGMKAVLWTSVLQTPVLLLGSIAVLIIGLIKVGGWSEVMEICR
                     AVPVNADGDTMVNLIRSHRDPEFPWTGVILGSAIIGFWYWCTDQYIVQRVLSAKNQKE
                     ARRGTIFAGYLKLTPVFIFLIPGMIAYALNQKGLIHLPSSDSAFAVLVKELLPVGFKG
                     LVVGGLLAALMSSLASLFNSSATLFTVDFYKHFRPQASERELVRVGRIATATIVILGV
                     LWIPVMKGLGKVLYAYLQDVQSLLAPGIAAVFLLGIASKKTTPKAGFIGLVTGFVLGM
                     TRLGLNVFKSSLNPESLIYKIFVAPNWLHYECIIFFIIIALMIVVSMVTPKADPGAIK
                     GLYFGSATPEERAITRASWNKWDVINSAIIVSVIVAFYIYFW"
BASE COUNT         2268 a         1628 c         1813 g         2651 t
ORIGIN      
        1 gaagaactgg ctgtttgagt tggtaaagcc tgcctccttc agcgcatcgc tcagagtaca
       61 ggcagttctg tgaatatggt gggctattga tttaaggcct tccggtccgt ggtactgggc
      121 atacatcccc gacataattg cgagcagtgc ctgtgctgtg cagatgtttg atgttgccct
      181 ttccctttta atatgctgtt cacgggtctg aagagccatt ctgagggcgg ggttgccctg
      241 tgcatcaacc gatatgccta ttattcttcc cggcatattt cttttgagtt cttctttagt
      301 ggcgaagtag cccgcatgag gtcctccgta gctcatcggc agtccaaagc gctggcttga
      361 gcccacaacc acgtcggcac cccattcacc tggaggggtg agaagtgcaa gactcatcag
      421 atctgctgcc acacaaagca gagctccggc agaatggatc tttaaagcaa aatcgcgata
      481 atcccttatc atgcccgaag ctgaggggta ctgaacgatg gcaccgaaaa aggtattgtc
      541 gggtgtgaac tcttcataat tacctgtcac aagttcgata cccagagggg ctgaacgcga
      601 tttcagaacg tcgagtgtct gaagaaaaat atcattgtca acaaagaact taacggctcc
      661 tgctcttaca gcttcttttg ggcgtgcatt gaacatcatt atcatagctt cagcagcagc
      721 agtagattca tccagaagtg atgcgttagc tatctgcatc ccggtaagct ctattatcat
      781 ggtctggaaa ttcagaagag cttcgagcct gccctgcgat atctcggcct gatagggagt
      841 gtacgaagta taccatgacg gattctcaag tacgttacgg attataactg acggcacagc
      901 taccccataa tatccctgtc cgatgaatga ccggtattgc ttattcattg ccccgagttt
      961 cctgatatgg ttaagatatt catgttcact cattgcagga ggaagattca ggggttttgt
     1021 cagccttatc gatgaaggga ttgttttatc aataagctca tcaagagaag agacacctat
     1081 tgttgcaatc attccatcta attcattatc ccgaggccct atatgccttt taacgaattt
     1141 gtctgaagtc attatgttat tgatatatag ttaaataaag ataaatattc ttgcgtttag
     1201 cacattgcta aaatagtaaa agtaatttat taataccatg aaaagtgtaa tgtcagatca
     1261 gccatggatt tcttttaagc tcattttcga tggttgttgc cggcccatgg cctgaataaa
     1321 ttattgttga agggttcagt ataagtattt tttcttttat gcttttaagc agagtatttt
     1381 gatttcctcc cggtagatct gttcttccga tactgccggc aaagatggtg tcgccggtaa
     1441 atatgcatga ttctttttcg cagtagaatg ctactcctcc tgctgtatgc ccgggtacat
     1501 gcattgtttt cagagaaata tttccgaatt caattatttc gccatcttca atgaaggctt
     1561 ctggttcggg tggttcatcc attttcagtc cgaagaattc tgcatgggca ggtgcatttt
     1621 tcaggtttgg catctcttct ctgtggcagt atgtgcgtag attgtatttt tcaaacatga
     1681 atttgttccc gaaaacatgg tcgaggtggc aatgggtgtt cagcagaagg accggtttaa
     1741 aatttttaag ttctataaaa cgcaccagtc gctgaaattc tttttcatta tagcagccac
     1801 agtcaataac agcacattcg cccgttgtgt cggccacgat ataggtgttt acttcaattg
     1861 gagaaaaaac aagccggaat acttccatga tataattggt tatccattca ctgtacctct
     1921 cagcatgggg acaaatacaa atgtgccatg ggaagagtag tcgtacgaat cggggcctgt
     1981 gcgttcgacc acggtcatta tctgataatc tccgccaccg agtggtacaa cgagtctgcc
     2041 gcctgtttta agctgctgca gcagtttatc gggtatttct ggtgctgcag ccgtaataat
     2101 gataccgtcg tagggtccat attccttttt accttcccat ccgtcaccga aaaagaaatg
     2161 aatattgtgg taacccagtt ctgcgattgt ctgctgggct ttcaggtaaa gctcccggca
     2221 tcgctcgatg gtgtagaccc tggcgcccat ttcagccaga atagccgcct gatagccgga
     2281 gccagtacca atctcgagaa ttttatctct cttttttacc ttcagcaggc tggtcattat
     2341 tgcaactgta tagggctggg aaatggtttg acccgcagct atgggaaaag ccttatcggc
     2401 atatgcatga tgcagaaaag caggatccat aaacagatgc cgtggtacgg ttctcatagc
     2461 ccgcaatacc tcttcgtcgg ttattccttt ttccctgagg ctctcgacaa gcttttccct
     2521 caggcctctg gcctcaaacg tatcctgcat cttttcaggt ttttctcagg cttattctac
     2581 tggattctta tttactccgg gcaaaaataa aaaaattatt tttgccggaa ccttttaaaa
     2641 atattccggc aattaaaaac aattttgtcc gttaatatta catgaagaaa accagattgg
     2701 cgtttatata tctgtttttt gacattgcag gttctgtagc tgcctggatt ttattctgca
     2761 tcatgacagg taccccgtct ggagccagca tgcaactgtc cggagagcat acgggttttt
     2821 atataaaaag ctctgctgct gtatcacttt cgtgggcttt aatctttttc cttaccgggt
     2881 tttattcagt atcattaaag aggtcaaggc ttcaggagct tttttattct cttgcggtta
     2941 ctttaacggg agtagtattt ctgtttattt ttttactggc cgagaggtat ttaactgact
     3001 attccacatt cctgcgctct ctcaccttat tattcacatg gcagtttctt cttacatata
     3061 ttccgaggac gattgtaact tcggttactg cccgnnnnnn nnnncacaga ggactggttg
     3121 gttataagac tctgattctt ggttcaaaga aaaaagcatt agagatttac aggaggataa
     3181 gagaggaggc aatcccgggt ggcaatattc ttacggggta cattattatt aatggtggag
     3241 caaatcataa gtccgaatgg gatcttgaat gcctgggaaa tgctgaagat atactgaagg
     3301 taattaatga gtatcagatt gaagaagtta tacttgccgt tgaggatgat gagttcgaaa
     3361 agcttctcga tattataggg cagcttcaat tcactgatgt tacaattaaa gcgattcctt
     3421 cgctaaaaga cctgttaacg ggacgggttg aacacacttc catatttgga actccattgc
     3481 tggagatccc taacaaaacc atgactcata cgcaggcaat tattaaacag ataattgatt
     3541 atgctgcttc aataatactt ttaattcttc ttattcctgt catccttgcc cttgccattg
     3601 caataaagct ttacgacgga gggcccatcc tgtaccggca ggaaaggatc gggcgaaatg
     3661 gaaagccatt tactattctg aagtttcgtt cgatgaagaa agatgccgaa agcacaggac
     3721 cgatgctttc ggggaaggac gatcccagga ttacaccatt aggaaggttc atgcgcaaac
     3781 acaggcttga tgaaatacct aaccttctga atgtgcttaa gggtgatatg tcgctcgttg
     3841 gtccacgccc ggaaaggcaa tattatattg agcaaatagt aaaaaaagcc ccacacttca
     3901 ggcgtcttct taaagttaaa ccagggataa catcatgggg tcaggtgaaa tatggttatg
     3961 cttcaaacat cgatcaaatg gtcgaaaggc ttgaatatga cctcatctat cttgaaaata
     4021 tgaatctgct tgtcgatctc aaaatcatta tctatacggt gatgattatt ttaaagggaa
     4081 aagggatgtg ataaaggttt aatagtaagg atgaaactaa ttatcagaga attacttcag
     4141 tgattggttt gtaaagaggt cccccaggca ttaatacact ttcgtaatat attagcttgt
     4201 ttaatttaac attttgaaat tcaacgtttt cgtgttctga tataatttcc ttaagttttt
     4261 cgttgtttct gagaaattta actcttccca gtgtcaggtg tggtttaaat tctctctctt
     4321 cagcagggat attcagggag gtcataattc ctgaaactat tttctgaaga tttagaagtg
     4381 tttcggcatt ttctattcct gcccatatca cacgggggtt actgataccg gggaagacac
     4441 ccaggccttt tattgtaaag cttatttccc ctgaacctga acatttttcc tgaaggttcc
     4501 tgctcatcag ttcgatattt tcctcttcgg tatcaccaag gaatgccagt gtaagatgca
     4561 taatggcagg atcgacccat tttatcgaat ccctgttgag gccgttacgg agaagtgcga
     4621 tggcttttct cagtttttca cctggttcgg ctttgactgc aacaaatgtt ctcctcattt
     4681 tttataaaaa acaaccggtc aaatataagg attagttttg ttgcttctcc ggaaagtttt
     4741 ttgccggaat tttgttcttc atttcttttt tacaacattt gcaaatcgta atatcattta
     4801 attttaatca attctgatgt acataaatct ataccagctt tctctttttc ttattttttg
     4861 ctttgcagtt ctggttttta ttctgctttt ttacattccc gcgccatatg gaaaatttat
     4921 gcgcaggggg tggggacctt cagtaaaatc tggcctggca tggatgataa tggaattccc
     4981 atcaccattt ctgatggcat atttttttct tacctcgaca cataaaaata ttgttcatta
     5041 cgtatttatt ctgatgtggc ttgcacatta tctataccgc acatttattt atcctttcat
     5101 gcaatcggga agaaaaaagc cttatccggt tgtagttgtt cttatggctg ttattttcaa
     5161 cgtattaaat ggttttgtaa acgggtatgg cgtgtttcat ctttattcat atgataagtc
     5221 gtggttgttt tcatggcagt ttattgcagg ttttgtatta tttgccagtg gttttataat
     5281 aaataaaaca gccgacagta aaatgatgca tctcagaaat aacaattcag gggaatatgt
     5341 gatacctcgc gggtggttat tcgagtacat ttcgtcgcct cattatttcg gtgagatagt
     5401 tgaatggtgt ggttgggcaa taatgacgtt ttcacttccc ggacttgctt ttgctgtttt
     5461 tacgtttgcc aacctttttc cgcgggctat agcctcacat aaatggtaca ggcagcactt
     5521 cagcgattac ccttcgggaa gaaaagctgt tattcctttt ataatctgaa aattctttaa
     5581 tttcaattct gttgatttta atgaaacagt tgtgtatatt ccgattttga gaaatcagaa
     5641 ttaattttac tgttgaatcg gattaatgat gataatagaa gaacttataa atactgatac
     5701 ttacaagtca cctgttccgg agcccgggtt caggaattgg cttctaaggg ataactggtt
     5761 ttattttatc ataaattttg gaaggatagt cttaagaacc cgcagggagg cgctggaagg
     5821 aagatacgat tatcctgagt ggatttattc atctcatgag atattgaggc ttctggaaaa
     5881 aattgggggt cgttttgata ttgaggggat gaataatatt tccagaatat cagaaccggt
     5941 aatatttgtg agcaaccaca tgagcactct cgaaacaatg atctttcctg gcattattgc
     6001 ccctcgcaga aaagtgacgt ttgtggtaaa ggagagcctt gttagaaatt atttcttcag
     6061 ggacgtaatg cgttcgagag accccattgt tgtaggcagg accgacccgc gcagggattt
     6121 tgaaaccgta atgagccagg ggcctctgct tcttgaaaaa ggaatatcaa ttattgtttt
     6181 cccccagagt accagaagca gggagtttat accatctgaa tttaacacaa tgggagttaa
     6241 actggcaaag aagacaaata aatatgtggt tcctgtggca attaaaacag acttctgggg
     6301 aaatggtaag attatcaaag aattcgggaa atttgaccac agaaaaacca tacatataaa
     6361 gtttggtgag cctttgaatg tgaaaggaac aggtaaagaa gaaaatcagg ctataataga
     6421 attcatccag ttgaatctgg aaaaatggga aaaggaacat aattaacaac ctttttcata
     6481 attgatattc gtcgatttgc ttcgataaat aatttttgtg aattttttta agtgtaatag
     6541 atacaattaa aatatttctt ttttagattt gaggaaaata aggggaaaag taaaaaattc
     6601 aagaatactc aacaggaacc attaattatt tattcaattt aaaatgaatc aaatatggca
     6661 actcttgact ggattgtttt aattcttttc tgcttaggac tcgtaggtat aatcgtctgg
     6721 gttgtaaaac agaagactga aacctcagct gattatttcc ttgcaggtcg tgatgctacc
     6781 tggctggcaa tcggagcatc tatttttgct tccaatatag gttcggaaca ccttgttgga
     6841 cttgcaggag caggtgcatc gagcggtatg gccatggcgc actgggagat gcatgcatgg
     6901 gttatactta ttctgggatg ggtattcgta cccttttatt caaggagtaa agttttcacc
     6961 atgcctgagt ttctcgaaaa gagatataac cggggagcaa ggtctttcct ttcggttatt
     7021 tcacttgtaa gctatgtcct tacaaaagtc gctgtaacag tatacgcagg cggtatagta
     7081 tttaaacagg ttttcggcat tgaatcgatg ttcggaatag attttttctg gatcagtgct
     7141 gtaggccttg tgattcttac cggtatttat actgtattgg gaggaatgaa agctgtgctc
     7201 tggacctctg tattacagac acctgttctt ctactgggtt caattgcagt cctgattata
     7261 ggacttataa aggtaggagg atggtcggaa gtaatggaga tatgcagggc tgtgccagta
     7321 aatgctgatg gtgataccat ggtaaatctt ataaggtcgc atcgcgaccc tgagtttcca
     7381 tggacaggtg ttattctcgg ctcagccata ataggcttct ggtattggtg caccgaccag
     7441 tatattgtac agagggttct ttcagcaaaa aatcagaaag aggcacgtcg tggtactatt
     7501 ttcgcaggtt atctgaaact tacccctgtc ttcattttcc ttatcccggg tatgattgct
     7561 tatgctctta accagaaagg tttaatacat ctcccaagtt ctgattcggc attcgcagta
     7621 ctggtcaaag aactcttgcc tgttggcttc aaggggctgg tggttggtgg tttactcgca
     7681 gcacttatga gttcccttgc atcattattt aactcatctg caaccctctt cacagttgat
     7741 ttctataaac acttcagacc acaggcaagc gaacgcgagc ttgtaagagt aggaagaatc
     7801 gctactgcaa ctatagttat tcttggcgtt ctctggattc ccgtaatgaa gggactgggg
     7861 aaagttctat atgcctacct tcaggatgtt cagtcactgc ttgcaccggg tattgctgcc
     7921 gtatttctcc tcggaattgc ttcgaaaaag accaccccaa aagccggatt catcggactt
     7981 gttactggct ttgtgcttgg catgacacgc cttggactta acgtattcaa aagctcactc
     8041 aatcctgaga gtttaatata taaaattttc gttgctccaa actggcttca ttatgaatgc
     8101 ataatattct ttatcatcat tgcactcatg attgtagtaa gtatggtgac accaaaagca
     8161 gatcctggag caataaaagg actctatttc ggttctgcaa cacccgaaga aagagcaatt
     8221 acgcgagcca gctggaacaa atgggatgtt attaattctg ctataattgt cagtgttata
     8281 gttgcatttt atatctactt ctggtaagaa attaaatcaa acagatatgg aaaacctcag
     8341 gaagtttgaa gcatggttcg tgaccggcag
//
LOCUS       DAOSNB010000082         8322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8524_length_8322_cov_4.332648, whole genome shotgun sequence.
ACCESSION   DAOSNB010000082 DAOSNB010000000
VERSION     DAOSNB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8524_length_8322_cov_4.332648"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLG42_04815"
     CDS             <1..752
                     /locus_tag="PLG42_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU30567.1"
                     /translation="SLMTRRPFRSPHHTISDVALVGGGTFPQPGEISLAHNGVLFLDE
                     LPEFKRSVLEVLRQPLEDRVITISRARFTVDYPANFMLVASMNPCPCGFHNHPEKNCT
                     CSAAMIQKYLSRISGPLLDRIDIHIEVIPVKYDKLSGNVSTDNSEKIRERVIKARAIQ
                     AKRFENISGIYTNSQMTPSLQRKYCYLDEAGSRLLKTAMDIRGLSARAYDRILKVART
                     IADLDESENIQASHISEAINYRNLDREGWAG"
     gene            834..1949
                     /locus_tag="PLG42_04820"
     CDS             834..1949
                     /locus_tag="PLG42_04820"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOU30568.1"
                     /translation="MNNLIRHIREKMTESRLMFAYRGVVTSENTTSLLTLLEKEMESS
                     EFGFQGRKRLFMFVLESLQNVSRHSKQDQFAEMSLVIYSKTYEGYTVTTGNVIENSQI
                     PNLKARLDAINNLNASEIRKVYREMLNTTEFSSKGGAGLGLIEMAKKTGNRLDYDFIK
                     IDDNYSYFILSKTVDSSGTGLSDRIPMTRFESEAVVQIEKLMSLNNIHLIWSGHFSTD
                     VSKEVLSFTETGLTEKSIKSSVRRKVFGILVEALDNIAKHSAPANMAEKFGMPVVLIR
                     LENNKDYVITTGNLIENEKIIQLKEKLDAINSCDREALKNLMKESLRSQRVSSDSTGN
                     LGLIEMARKSDGRLDYYFEPVNEIYSYFTLSVRVSEN"
     gene            complement(1953..2693)
                     /locus_tag="PLG42_04825"
     CDS             complement(1953..2693)
                     /locus_tag="PLG42_04825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOU30569.1"
                     /translation="MAGEKIFFASDFHLGLGAGTDTAGRERKVVEWLDEIAPSAKEIY
                     LLGDVFDFWWEYRYVVPRGFTRFLGTISFITDSGIPVHLFAGNHDMWIGKYLENECGV
                     ITHHEPLITEFDGKKFYLAHGEGLGSDHKAFKLLLRIFRNRPLQKLYSALHPRIGMGF
                     GHWWSKNSRLGKGISLEFMGEEKEDLIRYSKDLLEKEKIDYFIYGHRHLTLKHVLGNG
                     STVMILGDWIQQPNYAEWDGVTLTLKYY"
     gene            complement(2704..3900)
                     /locus_tag="PLG42_04830"
     CDS             complement(2704..3900)
                     /locus_tag="PLG42_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU30570.1"
                     /translation="MKKQGKTSGIIKYKHLYMVFFLLLAAFSCKESQKIIIEGKLEKP
                     EKGYIFINRIDINTPVPVDSVKIKRNGSFRLRINATLPEFYQVGKTGKGFITILAEPG
                     EKISINFRSEFLSRDYTVKGSPGTQKLMVLDSALAVTQRKIDSLNAEYNKAYGKPGFE
                     SIEKEINDAYLKLLKDQRMFNISFILKNLNSFASIKALYQRFDDQTYVLYDPRDIQFL
                     KIVSDTLNYHYPKSKQARALKANFEQEKEKLMMSQIEELAKKAPERKLDPELKDINGK
                     RVALSSLRGKYVLLAFWSAASSDCIAENLELKTLYNKYKSKGFEIYQINLDLNEDTWK
                     KAVQFDELPWISVREDDPTNPVNAMIYNVRILPANYLYSPDGEIIASNLHGKQLQLKL
                     QQIFGY"
     gene            complement(3935..5086)
                     /locus_tag="PLG42_04835"
     CDS             complement(3935..5086)
                     /locus_tag="PLG42_04835"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU30571.1"
                     /translation="MRKYIPLILVVLFSGCSNNSVTISGKLENAPEGEYLYLAEIKET
                     AVENVDSVKLEKNGNFSFKREIDIPVFYLLKQSNGNFFTLLAGPGEKIEVKAVYNRLN
                     EPVSVKGSEGTDKLLEYNKVLKSTTEKLRNLSEIYRENASSPDLPKVVETIDSTARVY
                     LSEINSYTKKFIDDNPGSLVTLIALYQQVAPRAYVLDPVKDINYFIKVDSVLYRRYPA
                     SGPVRALHEQVAALVEGVTSSGGTLLTPGNEAPDFTLPSPDGQMISLSSTRGKVVLLD
                     FWAAWCGPCRLENPNLVRAYEKYHNKGFEIFQVSLDRTREAWLKGIEEDKLGKWIHVS
                     DLKYWESSVVSLYHIEQIPNNYLLDRDGKIIAVNLRGEDLQSKLAEIFQ"
     gene            5277..7226
                     /locus_tag="PLG42_04840"
     CDS             5277..7226
                     /locus_tag="PLG42_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOU30572.1"
                     /translation="MGYIKFDKAKLVNLEYSLSREILRTNRAGSYSSTTIVNCNTRKY
                     HGLLVCPVDTFGGERHVLLSSLDVIVVNNDASFNIGIRKYKGDYYNPKGHKYLEDFDT
                     EVIPATTYRVGGVKLKEEKLLVHYEEQFLVRYTIVEASEPMKLQIRPFLAFRSIHDLT
                     HANMIANTKVEYIKNGIKIRLYEGFPWLHMQLSEKAEFIPVPDWYYGVEYIEEQKRGY
                     EYSEDLFTPGFFEVEAKEGDSIVFSATTSEEKPAGFKIKFTKTVKAKIPRSSFKNCLQ
                     NAAQQFIERRKGMTLIIAGYPWFGAWGRDTFISLPGLALAKHKLELYEDVFDTMIKRL
                     KNGLFPNTCGDDCAAYNSVDAPLWFFWALQNYVKQGGKNVWSKYGETAKSIISSYING
                     TDFNIHMRENGLIYWDAPGKAITWMDAVVNGEPVTQRKGYDVEINALWYNAICFALEM
                     AEKNNDNEFIEKYRHLPELIRKSFIEIFWNEEAGYLADYVNDEEGANMFIRPNMVIAV
                     SLPYSMLSTEQMKKVLDIVDKQLVTPRGLRTLSPRNQYYKGVYTGNQEQRDMAYHQGT
                     AWPWLLGPFCEGWLKVYNRQGVQKVKNLVYGFEEVMNEHGVSTISEIYDGDPPHTARG
                     AISQAWSVGEILRIINILETKYPDE"
     gene            7238..>8322
                     /locus_tag="PLG42_04845"
     CDS             7238..>8322
                     /locus_tag="PLG42_04845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU30573.1"
                     /translation="MRVLMFGWEFPPHISGGLGTASYGLTKGIVSLKDTEVIFVVPKA
                     WGDEDQSAVRLIGANNIPVAYRRVYYHGFKREITTYEVQSEIIPYTEPEEFWKLIKKR
                     EELQKSILVHTNQEGKIDFTGRYNGNLLDEIYKYSIVAAVLASELEFDIIHAHDWLAY
                     PAGISAMEVSGKPLVIHVHATEFDRSGGNVNPDVYNIEKRGMDAAAKIITVSNRTRDI
                     VISRYNINPEKVETVYNAVEPVDVAEHLHIRKGFDEKVVTFLGRITLQKGPEYFIEAA
                     NKVLKKMKNVRFVMAGSGDLMERMIRRAASLKITDRFHFTGFLRGKDVFTMLAMSDVY
                     IMPSVSEPFGISPLEAMMSNVPVIISK"
BASE COUNT         2475 a         1620 c         1795 g         2432 t
ORIGIN      
        1 tctcacttat gacccgccgc cctttccgct caccccacca tacaatatcc gatgtggcac
       61 ttgtcggcgg aggcactttc ccacagcctg gagaaatatc tctcgcccat aacggggtac
      121 tcttcctgga tgaactgcca gaattcaaaa gaagcgtcct ggaagtttta agacaaccac
      181 ttgaggatag agtgattaca atatccagag ccagattcac agtagattat ccggccaatt
      241 ttatgctggt agcatccatg aacccgtgcc cctgcggctt tcataatcat cctgaaaaga
      301 attgcacctg ctcggcagca atgattcaaa agtacctcag cagaatttcc ggtccacttc
      361 tcgaccgtat agacattcat attgaagtca ttccggtaaa atatgataaa ctttcaggaa
      421 acgtttcgac cgataattct gaaaaaatca gggagagggt gataaaagcc cgtgccatac
      481 aggcaaaaag atttgagaat atttcgggta tctatacaaa ttctcagatg acaccttcac
      541 tccagagaaa atattgctac ctcgatgaag caggtagccg actgctgaaa accgccatgg
      601 acataagagg gctttctgca agagcttatg accggatttt aaaagtggca cgcactatag
      661 cagaccttga cgaatcagag aacattcagg caagccatat atcagaagct attaactaca
      721 gaaacctcga cagggaagga tgggccggat aaaatcagta tatttttctg ccttttttgt
      781 taaatcaatg tttattccta aatttattct gtaaaatttt ctgctgacac ctgatgaata
      841 atcttatcag gcatattagg gaaaagatga cggaaagtcg tctgatgttt gcttaccgtg
      901 gagtggtgac aagtgaaaac accacctccc ttctcacact tcttgaaaag gaaatggaga
      961 gttctgagtt cggctttcag ggaagaaaaa gactctttat gtttgtgctt gagagcctgc
     1021 agaatgtttc acgccacagc aaacaagacc agtttgccga aatgtcgctg gttatatact
     1081 caaaaacata cgaaggctac actgttacaa caggcaatgt tatagaaaat tcacagattc
     1141 caaacctgaa agccaggctc gatgcaatta acaaccttaa tgcttcggag ataaggaagg
     1201 tgtaccgcga gatgctgaat actaccgaat tcagcagcaa aggaggagcc ggcctcggat
     1261 tgattgaaat ggcaaaaaaa acaggtaaca ggctcgatta tgactttatt aaaattgatg
     1321 ataattattc ctatttcatt ctgagcaaga cagttgattc aagcggcacc ggcttaagtg
     1381 acagaatacc aatgaccagg tttgaaagtg aagccgtggt gcagattgaa aagctcatgt
     1441 cactcaataa cattcacctg atatggtctg gccatttttc tactgatgta agcaaagaag
     1501 tcctttcctt tacagaaaca gggctcacgg agaaaagtat aaaatcttca gtcaggagaa
     1561 aagttttcgg tatactggta gaagcactcg acaatattgc caaacacagt gctcctgcaa
     1621 atatggctga gaaattcgga atgccagtag ttttaataag actcgagaat aacaaagatt
     1681 atgtgattac cacaggcaat ctgattgaaa atgaaaagat aattcagctc aaagaaaaac
     1741 tcgatgcaat taacagttgc gacagggaag cacttaagaa ccttatgaag gagtcactgc
     1801 gcagccagcg tgtttcttca gatagtacag gtaatctggg gttaattgaa atggccagaa
     1861 aatcagatgg cagactcgat tattattttg aaccggtaaa tgaaatatat tcatacttca
     1921 ctctatcggt gagggtctct gaaaattgat tcttaataat atttcagagt cagggtcaca
     1981 ccatcccatt cagcataatt tggctgctgt atccagtcgc ccaggatcat gaccgttgag
     2041 ccgttgccaa gaacatgttt taaagtaaga tgccggtgac cataaatgaa gtagtctatc
     2101 ttttcttttt ccagtaaatc ttttgaatac cttataagat cttctttttc ttcccccata
     2161 aattccaggg aaataccctt gccaagcctg ctgttttttg accaccagtg accgaagccc
     2221 atacctatgc gggggtgcag ggcggaatag agcttttgaa ggggtcggtt acggaaaata
     2281 cgtaaaagta gtttgaaagc tttatggtct gaccctaacc cttccccatg ggcaagatag
     2341 aattttttac cgtcaaactc tgtaatgaga ggctcatgat gagttataac accgcattcg
     2401 ttctcaagat attttccaat ccacatatcg tgattcccgg caaaaagatg tacgggtata
     2461 cccgaatcag taataaatga aattgttccc agaaaccttg taaatccgcg gggaaccaca
     2521 tatctgtatt cccaccagaa atcaaacaca tcacccagaa gatatatttc ctttgccgat
     2581 ggagcaattt catcaagcca ttcgaccact tttctttccc ttccggcagt gtcggtcccg
     2641 gcccctagcc cgagatgaaa gtccgatgca aagaatattt tctcccctgc cactttattg
     2701 aagttaatat ccaaaaatct gctggagttt tagttggagt tgcttgccat gaagatttga
     2761 tgcaattatc tcgccatccg ggctgtagag atagtttgca ggtaaaatcc ttacattgta
     2821 tatcattgca ttcaccggat ttgtcgggtc gtcttccctt acacttatcc agggaagttc
     2881 atcaaattgt acggcttttt tccatgtatc ctcgttcaga tccaggttaa tctggtaaat
     2941 ctcaaatcct ttacttttat acttgttata aagggtttta agctcaaggt tttcagcaat
     3001 gcaatcggat gatgcagccg accagaaggc aagcagaaca tatttacccc tgagtgatga
     3061 cagagctact cttttcccgt taatgtcttt taactcgggg tcaagtttcc tttcgggagc
     3121 tttttttgca agctcttcta tctgactcat catcagtttt tctttttcct gctcgaaatt
     3181 ggctttcaga gcccttgcct gcttcgattt aggataatga tagttgagag tatcggatac
     3241 aattttcaga aactgaatat cgcgtggatc gtaaagtaca tatgtctggt cgtcaaaacg
     3301 ctgataaagg gcttttatag aggcaaatga attcaggttt ttaagaatga aggagatgtt
     3361 gaacattctc tgatccttca gaagctttaa atatgcatcg ttgatctctt tttctatcga
     3421 ctcaaaaccg ggtttgccgt aagctttgtt atattctgca ttcagggaat cgatttttct
     3481 ttgtgttact gccagtgctg aatcgagcac catcagcttc tgtgttcccg gtgaaccttt
     3541 aactgtataa tcccgcgaga gaaactctga gcggaaattg atgctgattt tttccccggg
     3601 ctctgcaagt atagttataa accctttgcc tgttttgccg acctggtaaa actcaggcag
     3661 tgttgcattt attcttaacc tgaaagaacc gtttctcttt atttttactg aatcaaccgg
     3721 aacgggagtg ttgatatcta ttctgttgat gaaaatatat cctttttcgg gtttttcgag
     3781 cttaccttct ataattattt tctggctttc tttgcatgaa aaagctgcta aaagcaggaa
     3841 gaataccatg taaaggtgtt tgtattttat gatgcctgag gtttttcctt gctttttcat
     3901 tgatagcgtc tgattctgat tttaaaatta tttatcactg aaatatttca gcaagtttcg
     3961 actgaaggtc ttcacccctg aggttaacgg ctattatttt cccgtccctg tcgagcagat
     4021 agttattggg gatttgttca atgtgatata gtgataccac tgatgattcc cagtatttca
     4081 ggtcacttac atgtatccat tttccgagtt tgtcttcctc tattcccttg agccatgctt
     4141 cacgggtacg atcgagagag acctgaaata tttcaaagcc cttgttgtgg tatttttcat
     4201 atgctctgac aagatttgga ttttctaacc ggcatggtcc gcaccatgca gcccagaagt
     4261 cgagtagtac aactttaccc cttgttgatg aaagagatat catttgcccg tcgggcgagg
     4321 gaagagtaaa atcaggggct tcgttaccag gtgtcagcaa tgttccacct gaagatgtta
     4381 caccctcaac gagggctgct acctgctcgt gaagcgccct taccggtccg gaagcgggat
     4441 aacgcctgta aagaacggaa tcaaccttaa tgaaatagtt tatatcttta acagggtcaa
     4501 gtacataggc ccttggagcg acctgctggt aaagagcaat aagcgtaaca agagatcccg
     4561 ggttgtcatc aataaacttt ttggtataac tgtttatctc cgacaggtaa accctggcag
     4621 tgctgtcgat agtctcaaca acttttggca ggtcaggact acttgcattt tctctatata
     4681 tttctgaaag attccgcaac ttttcagttg tacttttaag aaccttgtta tattcgagaa
     4741 gcttgtctgt cccttcggaa ccctttacgg aaacaggttc gtttaacctg ttatagacag
     4801 cttttacctc aattttttca ccgggcccag ccagtagagt aaagaaattt ccattagact
     4861 gttttaaaag ataaaagacc ggtatatcta tctctctttt aaaggaaaaa tttccgtttt
     4921 tttcgagttt cacagaatcc acgttctcaa ctgcagtttc ttttatttcg gcaaggtaaa
     4981 gatactcacc ctcaggagca ttctcgagtt ttcctgaaat tgttacactg ttattgctgc
     5041 aacctgaaaa taaaacaacc agtattaaag gaatatactt tctcatgttg atatttgtat
     5101 tacttttgaa aatttaattg tttgcaaaga tattctttct ttttattgcc tgaaagtatt
     5161 tgataaaatt aataaatgca ttatttttca caggtttatg ataatttgcc accggtgatt
     5221 ttggagttaa aataaaaaga attatcttaa aagagttaaa agtatattta aattttatgg
     5281 gatatattaa gttcgacaaa gcaaaactgg ttaatcttga atattctcta agccgtgaga
     5341 ttttgaggac aaacagggct ggaagttatt cgtccacaac gattgttaac tgcaatacac
     5401 gtaagtatca cgggttgctt gtctgtcctg ttgacacatt tggtggggaa aggcatgttc
     5461 tgctttcatc tctcgatgtt attgtagtta ataacgatgc ctcattcaat attggaatca
     5521 gaaaatataa aggtgattat tataacccca aggggcataa atatcttgag gattttgaca
     5581 cagaagttat cccggcaaca acatacaggg tgggaggggt aaaactaaag gaggagaaat
     5641 tgttggttca ttacgaggag cagtttcttg taagatacac tattgttgag gcttcagagc
     5701 cgatgaaact gcagatacgt ccttttctgg ccttcagaag catccacgat cttacgcatg
     5761 ccaacatgat tgcaaataca aaagttgaat atataaagaa tggaataaaa ataaggcttt
     5821 acgaaggctt cccatggctt catatgcagc tttcggaaaa agcggagttt atacctgtac
     5881 ccgactggta ttatggagtc gaatacatag aggaacagaa aagaggttat gaatattccg
     5941 aggatctttt tacaccggga ttttttgaag ttgaagcaaa agaaggtgac agcatagtct
     6001 tttcagcaac tacctctgaa gaaaaacctg caggttttaa aataaagttt acaaaaacag
     6061 taaaagctaa gataccgaga agcagtttca aaaattgttt gcagaatgct gcacaacagt
     6121 ttattgaaag aagaaaaggg atgacactga tcattgctgg atatccatgg tttggagcat
     6181 gggggcgtga tacctttata tcattgccgg ggcttgcact tgcaaagcac aaacttgagt
     6241 tatatgaaga tgtatttgat acaatgataa aaaggttgaa aaatggtctt ttccccaata
     6301 cgtgcggcga tgattgtgca gcttataatt cagttgatgc acccttgtgg ttcttctggg
     6361 ctcttcagaa ttatgttaaa cagggcggta aaaatgtatg gagcaaatac ggtgaaacag
     6421 cgaaatcaat tataagctcc tatattaacg gtaccgattt caatattcac atgcgtgaaa
     6481 acgggctcat ttactgggat gctcccggga aagctattac ctggatggat gccgttgtta
     6541 atggtgagcc ggtaactcag agaaagggat atgatgttga aattaatgcc ctgtggtata
     6601 atgcaatatg ttttgctctt gagatggcag aaaaaaacaa tgataatgag tttattgaga
     6661 aataccgcca ccttccggag cttataagaa agtcgtttat tgaaattttc tggaatgagg
     6721 aggctggata cctggccgac tatgtcaacg acgaagaagg tgctaatatg tttatcaggc
     6781 ccaatatggt cattgctgtt tcgctcccgt actcgatgct gtccactgag caaatgaaga
     6841 aggttctgga tattgtagac aaacaacttg ttactccccg cggcctgaga accctttcgc
     6901 cccgtaacca gtattacaag ggtgtttaca cagggaacca ggaacagcgt gatatggctt
     6961 accatcaggg tacggcatgg ccctggcttt taggaccttt ctgcgaagga tggctgaaag
     7021 tttataaccg acagggcgtg cagaaggtta aaaatcttgt ttatggattt gaagaggtga
     7081 tgaatgagca tggtgtgagc accatttctg agatttatga cggagaccct ccgcatactg
     7141 caaggggtgc aatttcacag gcatggagcg ttggtgaaat tttgagaatt attaatattc
     7201 ttgaaactaa atatcctgac gaataaaagg tgttgttatg cgtgttttaa tgtttggctg
     7261 ggaatttccg ccccatatct ccggtggcct tggtacggca agctatggcc ttactaaagg
     7321 cattgtctcc ctgaaagata cggaggtaat atttgttgtt ccaaaagcat ggggtgatga
     7381 ggaccagtcg gccgtcaggc tcataggagc taacaatata cctgtggcct accgccgggt
     7441 atattaccac ggttttaagc gagaaataac aacatacgag gtgcagtcag aaataatccc
     7501 ttataccgaa cctgaagagt tctggaaact tataaaaaag agggaggaat tgcagaagag
     7561 tatccttgta catacaaatc aggaaggcaa aattgatttt accggaaggt ataatggaaa
     7621 cctgcttgat gaaatttaca aatactcaat agttgctgct gttcttgcct ctgaactaga
     7681 attcgatatt atacatgctc acgactggct tgcttatccg gcaggtattt ctgcaatgga
     7741 agtatcgggt aaacctcttg tgattcatgt tcatgcaact gagttcgaca gaagcggggg
     7801 aaacgtcaat cccgatgttt ataacatcga gaagcgcgga atggatgcag cagcaaagat
     7861 tattactgtg agcaaccgta caagggatat agtaatatcc aggtacaata tcaatccaga
     7921 aaaagttgaa acagtctaca atgctgtaga acctgttgac gttgccgagc atctacatat
     7981 aagaaaaggg ttcgatgaaa aggtcgtgac tttcctgggc aggataacac tgcagaaggg
     8041 accagaatat tttattgaag cagcaaataa ggtgctgaaa aagatgaaga atgtaagatt
     8101 tgtaatggct ggttcgggcg acctgatgga aaggatgata agaagagcag catcgttgaa
     8161 gattactgac aggttccatt ttacggggtt tctgagagga aaggatgtct ttaccatgct
     8221 ggcaatgagc gatgtgtata taatgccatc tgtttcggag ccttttggaa tttctcccct
     8281 cgaggcaatg atgtcgaatg ttccggtaat aataagtaaa ca
//
LOCUS       DAOSNB010000083         8290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8575_length_8290_cov_5.295689, whole genome shotgun sequence.
ACCESSION   DAOSNB010000083 DAOSNB010000000
VERSION     DAOSNB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8575_length_8290_cov_5.295689"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PLG42_04850"
     CDS             <1..874
                     /locus_tag="PLG42_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOU30574.1"
                     /translation="LPLIIVTYVTGLGIEFIAAQIRGHEINEGFLVTGLLIPLIVPID
                     IPLWMLALATAFAVILGKEVFGGTGMNVFNPALLARAFLFFSYPGWMSGDRVWIEGLT
                     EGKNVVDGFSGATPLGLLMQNQTDALPSLKEMFIGTIPGSVGETSTLAILIGAAILII
                     TGIGSWKIMTGVFAGGIVMGLLFNLIGANQYMELPFYYHLVMGGFAFGAVFMATDPVT
                     ASQTETGKWIYGFLIGLLSIFIRVINPAYPEGVMLAILLMNAFAPLIDYYVVQANIKR
                     RLKRARVSLNTGAK"
     gene            871..1602
                     /gene="nqrC"
                     /locus_tag="PLG42_04855"
     CDS             871..1602
                     /gene="nqrC"
                     /locus_tag="PLG42_04855"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOU30575.1"
                     /translation="MKEFSNRYIFIFSLAMVVIVATILSLAATLLQPRQARNLELEKK
                     KSILESINVQADFSNADELYSKYITESFVLDYKGNIKEGEDAFNVELRNEQRKPLEQQ
                     SLPVFIAIPESGERITIIPLEGKGLWGPIYGYISLKPDMNTVYGVTFDHKGETPGLGA
                     EINTPWFEKQFIGKQIFEEGKFVSIKVVKASSKKAEGGGADATSGSTHGVDAISGGTI
                     TSKGLEAMLYDCLVKYEDYLRKNAK"
     gene            1606..2259
                     /locus_tag="PLG42_04860"
     CDS             1606..2259
                     /locus_tag="PLG42_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOU30576.1"
                     /translation="MSATEKEPLFSAKNIKLLTGPLGKNNPITVQVLGICSALAVTVK
                     LKPALVMALSVTVVVAVSNVVISALRSHIPDRIRIIVQLVVIATLVIIVDQLLKAYAF
                     DVSKQLSIFVGLIITNCIIMGRLEAFALSNKPWQSFLDGVGNGAGYGIILIVVAFFRE
                     LLGSGSIMGYPVIQKLGLYSAGYENNGMMILPPMALILVGLIIWFQRSRDKSLIEKK"
     gene            2272..2889
                     /gene="nqrE"
                     /locus_tag="PLG42_04865"
     CDS             2272..2889
                     /gene="nqrE"
                     /locus_tag="PLG42_04865"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOU30577.1"
                     /translation="MENLLNIFIKSIFIDNMIFAYFLGMCSYLAVSKTVKTSVGLGIA
                     VTFVLVITVPVNYLIYNYLLKKGALAWLGKGFADVDLSFLSFIIFIAVIATMVQIVEM
                     IIEKFSPALYNSLGIFLPLIAVNCAILGASLFMQEREYHNIGEVTAFALGSGIGWFLA
                     IVCLAAIREKLRYSNVPGPLKGLGITFILTGLIAIAFMGMMGIKL"
     gene            2909..4162
                     /gene="nqrF"
                     /locus_tag="PLG42_04870"
     CDS             2909..4162
                     /gene="nqrF"
                     /locus_tag="PLG42_04870"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOU30578.1"
                     /translation="MILAFTTAGTVISSIVVFLFVTILLVVVLIYAREKLTPRGKVKL
                     QINDRQMEVSPGSNLLFTLSSNGIYLPSACGGGGTCGMCKCQVLSGGGAILPTETGFF
                     TRKEQLNNWRLGCQVKVRQDMVVRVPEAVLGIRKWECEVISNRNVATFIKEFVVKLPE
                     GESLEFEPGGYIQIDVPKYEASFRDFDIDEQYRADWDKYGMWDLKVKNTTETFRAYSM
                     ANYPAEKNVIKLNIRIATPPWDNEKKRFKNVPPGICSTYIFSRKPGDKVMISGPYGEF
                     HIKHTDAEMVYIGGGAGMAPLRSHIFHLFKTLKTTRKVSYWYGARSLREVFYQEEFEE
                     IAKNFPNFSFHLALSEPLPEDNWKGYTGFIHQVLYDNYLSKHEAPEDIEYYFCGPPQM
                     NNAVLKMLNDLGVPKENIAFDDFGI"
     gene            4400..5410
                     /locus_tag="PLG42_04875"
     CDS             4400..5410
                     /locus_tag="PLG42_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOU30579.1"
                     /translation="MRKLVILIILSSLVLFSCRDKDPFIRIKGSTQGTSFSIICERKR
                     SVSENKVNRGISDILTRIDNSLSVYNSSSVISRMNRNEDVVADRYFTEVFELAQEVSE
                     LTGGAFDITVGPLVRAWGFGPEARAKFDTTKLDSLMQLVGYRKVRLENGKLVKENPGI
                     ILDVNAIAQGYTVDVISRYFDSLGVKNYLIEVGGEVRVKGTKGGKLWRIGIDRPYENN
                     LLPGADLQAVIELKDRSLATSGNYRKFYEENGVKYSHTIDPHTGYPARSRLLSSTILS
                     SECAVADAVATATMVMGLEKTIEFLENHPEFEAFLIYSDTSGYFRTWASESLKSSITE
                     EN"
     gene            complement(5413..5637)
                     /locus_tag="PLG42_04880"
     CDS             complement(5413..5637)
                     /locus_tag="PLG42_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30580.1"
                     /translation="MFIKILIISLILVAAAIIFLGVKIFFTRKGKFPEYHVGSNHEMR
                     KRGITCAKSTDIGYSASGENGCAACRQGIL"
     gene            complement(5667..7430)
                     /locus_tag="PLG42_04885"
     CDS             complement(5667..7430)
                     /locus_tag="PLG42_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30581.1"
                     /translation="MERSTLRIEGNKAILYIRERMCETPEELLTSSKFRTVLELCLRK
                     LEKNNSPLLGIFEDSKVTDRGIDRLIRTLILLEKYKCEILPHISEDSAEFIRDRLLLN
                     SFVEYLYDFWRHFDRFIINDSEGDRLDKRPYRTFNETIEHLTHLVRTTYRHIQENITG
                     KHPMVYRQVTAGGEMAVISIPQKIPYIGDIYRKLDHIQIIRQMLIYPPLVLQQPMNKR
                     TGHFRKIAVNPLELFHIDSSEYLCYPAMVGKLLIMVVFHISFFELGFSLCNLFEIADD
                     EQLKRKPDAVYIYGTPPDVLNGLADYQTVFYEDAENGIFVGAVPAKPEFGYFGYLKKM
                     ILTLHNAIVMKEKKMPFHGALVKILLQGGKEATVLMIGDTGAGKSETLEAFRVLGEKE
                     IRQLIIISDDMGSIEFDRDGFPVGYGTEIGAFLRLDDLQPGYAYGHLDRSIIMNPSQV
                     NARIIIPVTSYETVIRGHKIDYILYANNYEETGPDKPIIERFNSPEEALAVFREGKVM
                     SKGTTTSSGIVSSYFANIFGPTEYKNLHDEIAKRYFEVFFRKGIFVGQMRTKLGIPGC
                     ETSGPHDAATELLKIITNSEG"
     gene            7794..>8290
                     /locus_tag="PLG42_04890"
     CDS             7794..>8290
                     /locus_tag="PLG42_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOU30582.1"
                     /translation="MKDDFDFFSILPEDKIPEKGCVLISEPFLPDSFFNRTIVFLTEH
                     NEDGSVGFILNKKLDLRVCDAIEGFDNCEDYLSMGGPVAPDTLHYIHTLGDAIPKSVH
                     VYNNIYWSGDLEAIRKMYSFNKIKDHQIRFFLGYSGWAAGQLKRELKENSWVIARISP
                     EVIMT"
BASE COUNT         2345 a         1565 c         1943 g         2437 t
ORIGIN      
        1 gctgccactg attattgtca cttacgttac gggtcttgga attgaattta ttgcagcgca
       61 gataagagga catgagatta atgaagggtt ccttgtaacg gggcttctta taccgcttat
      121 agttccgatt gacatacctc tgtggatgct tgctcttgcc acggcctttg cagtcattct
      181 ggggaaggag gtttttggcg gaacgggcat gaatgtgttt aaccccgctc tcctggcaag
      241 ggcttttctg tttttcagtt atccggggtg gatgagtggt gacagggtat ggattgaagg
      301 tctgacagaa ggcaagaacg ttgttgatgg tttcagcgga gccacacctc tcggacttct
      361 tatgcagaat caaacagatg cactgccttc attaaaggaa atgtttattg gtactatccc
      421 tggttcggtg ggggagactt caacgctagc gatactgata ggcgcagcca tactgattat
      481 aaccggcatc ggaagctgga agataatgac cggtgttttt gcgggcggaa tagtgatggg
      541 acttctgttt aatctgatag gcgcaaatca gtatatggag cttccattct attatcatct
      601 ggttatgggc gggttcgctt tcggcgcagt ctttatggca accgacccgg ttacagcctc
      661 tcaaactgaa acaggaaaat ggatatacgg gttcttaata ggcctccttt caatattcat
      721 aagggttata aaccctgcct accctgaagg agtaatgctg gcgatactgc tcatgaatgc
      781 ttttgcaccc cttattgatt actatgtggt gcaggcaaac attaaaagaa ggctcaaaag
      841 agccagggtc tcacttaata caggagcgaa atgaaagagt tcagcaacag atatattttt
      901 attttctcac tggcgatggt tgtgatagtt gcaaccatat tgtcgctggc tgcaacgctt
      961 ctgcagccaa ggcaggctag aaacctcgag ctggagaaga aaaaaagcat tcttgaatcg
     1021 ataaatgttc aagcagactt ctccaacgca gatgagctgt acagcaaata catcactgaa
     1081 agctttgtac ttgactataa aggaaatata aaggaaggtg aggatgcttt caatgtggag
     1141 ctcaggaatg agcagcgaaa acctcttgag cagcagagcc ttccggtttt tatcgctatc
     1201 ccggagagtg gagaaaggat tactattata cctcttgaag gcaagggatt gtgggggcca
     1261 atttacggtt atatctcact taaacctgat atgaatactg tttacggcgt aacgttcgac
     1321 cataaaggtg agacacccgg attgggtgct gaaatcaata cgccgtggtt tgagaagcag
     1381 tttattggga agcagatttt tgaggaagga aagtttgtct ccataaaggt tgtaaaagca
     1441 agcagtaaaa aggctgaggg tggaggtgct gatgctacat cgggaagtac acacggagtt
     1501 gatgcaattt cgggcgggac gataaccagc aagggccttg aagccatgct atacgattgc
     1561 cttgttaaat atgaagatta tctcagaaaa aatgcaaaat aatatatgag cgcaacagaa
     1621 aaagaacctc ttttttcagc aaagaacata aaattgctaa ccgggccgct cgggaagaat
     1681 aatcccatca cggttcaggt acttggcata tgttcggcac ttgcagtaac ggttaagctt
     1741 aaaccggctc tggttatggc actttcggtt actgttgtag tagcagtttc aaatgttgtc
     1801 atttcagcat taaggtcgca catccctgac aggatccgca ttatagtgca gcttgtggtt
     1861 attgccacac ttgtgataat tgtggatcag ttgcttaaag cctacgcatt tgatgtgagc
     1921 aagcagcttt cgatttttgt ggggctgata ataacgaact gcatcataat ggggcgtctc
     1981 gaagcttttg ccctctccaa caagccatgg cagtcgtttc tcgatggggt gggcaacggt
     2041 gcaggctacg gaataattct tatcgtggtg gcatttttca gggaattact cggctcggga
     2101 agcattatgg gttacccggt aatacaaaaa ctgggactct attctgctgg ttatgagaat
     2161 aacggtatga tgattcttcc gccaatggct cttatacttg tcggacttat catctggttc
     2221 cagaggagcc gagacaaaag tcttattgaa aaaaaataaa aacggcctga tatggaaaat
     2281 cttctaaata ttttcataaa gtccattttt atcgacaaca tgatattcgc atactttctt
     2341 ggtatgtgtt catatcttgc tgtctcaaaa acagttaaga cttctgtagg actggggatt
     2401 gccgtaacat tcgttcttgt aataactgtt ccggtcaact atcttattta taattatctg
     2461 cttaagaaag gtgctctggc gtggcttggt aaaggatttg ccgacgttga cctgagtttt
     2521 ctttctttta taattttcat tgcagtgatt gccacaatgg tgcagattgt ggagatgata
     2581 attgaaaagt tcagtccggc actttataac tcactcggga ttttcctccc gcttattgct
     2641 gtaaactgtg caatacttgg agcatctctt ttcatgcagg agcgtgaata tcataatatt
     2701 ggcgaggtaa cagcctttgc acttggttcc ggcataggtt ggttcctggc aattgtatgc
     2761 cttgcagcaa tacgagagaa actgcgttac agcaatgtgc ccgggccact gaaaggactg
     2821 ggtatcacat ttatacttac agggctgata gcgatagcat ttatgggaat gatgggaatt
     2881 aaactttaat ctaaaccact ctgccgatat gattttagca ttcacaactg caggaactgt
     2941 tatctcaagc attgtggtat ttctttttgt aaccatactg cttgttgtag ttctaatcta
     3001 tgcccgtgaa aaacttactc ccagggggaa ggtaaagctt cagattaacg acagacagat
     3061 ggaggtgtcg cccggttcga accttctttt tacactctca tcaaacggca tttacctgcc
     3121 gtcggcatgc ggcggtggtg gcacatgtgg aatgtgcaaa tgtcaggtac tttcaggcgg
     3181 aggggcaata cttccaacag aaaccggttt cttcacgcgc aaggaacaac taaataactg
     3241 gaggcttggt tgccaggtga aggtaaggca ggatatggtg gttagagtac ctgaggccgt
     3301 ccttggcata aggaaatggg aatgcgaagt gatatcaaac cgcaacgttg ccacattcat
     3361 aaaagagttt gtagtgaaac tgcccgaagg agaatcactg gagtttgaac ccggaggtta
     3421 tatccagatt gatgttccga agtatgaggc aagtttcagg gatttcgaca tcgacgaaca
     3481 atacagagcc gactgggata aatacgggat gtgggatctg aaagttaaaa ataccactga
     3541 aaccttcagg gcgtattcca tggcaaatta cccggccgag aagaatgtta taaaacttaa
     3601 cataagaata gcaacgcccc catgggacaa tgagaagaaa cgattcaaaa atgttcctcc
     3661 cggtatctgt tcaacatata tcttttcgag gaagcctggc gataaggtga tgatatcggg
     3721 tccttatggt gaatttcaca taaagcatac cgatgccgag atggtttata taggcggagg
     3781 ggcaggtatg gcgccattgc gctcacatat ttttcatctt ttcaagaccc tcaaaaccac
     3841 caggaaagtc tcatactggt acggtgcacg ttctctgagg gaagtgttct atcaggagga
     3901 gttcgaagag attgcaaaga atttcccgaa tttcagtttc catcttgcac tttctgaacc
     3961 tttacctgag gacaactgga aggggtatac aggctttatc catcaggtgc tttatgataa
     4021 ttatctgtcg aaacatgagg cacccgaaga tattgaatat tatttctgcg ggccaccaca
     4081 gatgaacaat gctgtgctga aaatgctgaa tgacctcggt gttccgaaag aaaatatcgc
     4141 atttgacgat tttggtatat aaaaaagaaa ccccgcctgg cggcggggac aatcaattta
     4201 acaggggaaa ggggaatgtt aaatcgaatg ccggttctga taaccgggtt ttctgataac
     4261 aaatttcaga tatcttatgc aattttgaaa gatgattttg acaagtggcg aatatatatt
     4321 gacgaatgcc acttttttgt tgaggaaagt tttttaaaga cttcagcctt tatattttaa
     4381 aaaatataaa taattgatag tgagaaaatt agtaatacta ataatcttat cctccctggt
     4441 actcttttcg tgcagggata aagatccatt tataaggata aaaggttcaa cccagggtac
     4501 ttcgttcagt atcatatgcg aaagaaaacg gagtgtgtca gaaaataagg ttaacagggg
     4561 tatatcggat attttaacac ggatagacaa ttcgctttcg gtttataaca gttcatcggt
     4621 tatttcgagg atgaacagga atgaggatgt ggttgccgac aggtatttta cagaagtttt
     4681 tgaactagca caggaggtat cggagttgac aggaggggct tttgatataa ctgttggtcc
     4741 actggttaga gcatggggtt ttggaccgga agcaagggca aagtttgaca caacaaaact
     4801 cgatagcctt atgcaactgg taggatacag gaaggtgagg cttgaaaatg ggaagctggt
     4861 taaggagaat cccggaataa tactggatgt caatgcaata gcccaggggt atactgtgga
     4921 tgtaatcagc aggtattttg attcgctggg agttaagaac tatctgattg aagtaggagg
     4981 tgaggtaagg gttaaaggca caaaaggggg caaactctgg aggattggaa ttgacaggcc
     5041 atacgaaaat aatcttttac caggcgctga ccttcaggca gtaattgaac ttaaagaccg
     5101 gtcacttgca acatctggta attacaggaa attttatgag gaaaacggag taaaatattc
     5161 gcatactatc gatccacaca caggttatcc ggcgcgcagc aggcttctca gttcaacaat
     5221 attaagcagt gaatgtgccg tggctgatgc tgttgcaacg gctacaatgg tgatgggtct
     5281 ggaaaagacg atagagtttc ttgagaacca cccggagttt gaagcatttc ttatttattc
     5341 tgatacttca gggtatttca ggacatgggc gagtgaatcg ctgaaatcaa gtataactga
     5401 agaaaattag cattacagaa taccctgcct gcaggcagca cagccattct ctccggaagc
     5461 agaataaccg atatcggtgc tttttgcgca tgtgattcct cttttcctca tctcatggtt
     5521 gcttcctacg tgatattcag gaaatttacc tttacgtgta aaaaaaattt ttacacccag
     5581 aaagattatg gctgcagcga cgaggattaa tgatataatc agaattttta taaacatttt
     5641 tctgagttat tttttcagca aggttttcat ccctctgaat ttgtaataat tttcaacagt
     5701 tctgttgcgg catcatgtgg ccctgatgtt tcacacccgg gtataccgag ctttgttctc
     5761 atctgcccta caaagatacc ttttctgaaa aatacctcaa agtatctttt ggctatttca
     5821 tcgtgcaggt ttttgtattc agttggcccg aaaatattgg caaagtatga gcttacaata
     5881 ccgctcgatg ttgtggtgcc tttgctcatt accttgcctt cgcggaatac agccagagct
     5941 tcttcgggag agttgaatcg ttcaatgata ggtttgtccg gtcctgtttc ttcgtagtta
     6001 ttagcgtaaa gaatataatc aattttgtgt ccccttatca ctgtttcata agaggttaca
     6061 ggtataataa ttcttgcgtt cacctggctc gggttcatta ttattgacct gtcgaggtgg
     6121 ccataagcat atcctggctg aaggtcgtcg agcctgagaa aggctccgat ttctgttccg
     6181 tagccaacag ggaagccgtc gcggtcaaat tctattgagc ccatgtcgtc agatattatt
     6241 atcagctgcc tgatttcctt ctcgccgaga accctgaagg cttcgagagt ttctgacttt
     6301 cctgcgcctg tatctccgat catcagaact gttgcttctt ttccaccctg cagaagtatt
     6361 tttacaagtg ccccgtggaa aggcatcttc ttttctttca tgacgatagc attatgcagg
     6421 gtgagaatca ttttcttcag gtatccgaaa tatccgaact ccggctttgc aggtacagct
     6481 cctacaaata ttccgttttc agcatcttcg taaaaaacag tctggtaatc ggcaagaccg
     6541 ttgaggacat ccggaggggt tccatagata tagactgcat cgggttttct tttaagctgt
     6601 tcgtcgtcag ctatctcgaa aaggttgcaa agagaaaatc ccagttcaaa gaatgagata
     6661 tggaaaacta ccattatgag aagtttcccg accatggcag gataacagag gtattcactg
     6721 ctatcgatat gaaagagttc cagtggattt accgcaatct ttctgaagtg tccggtcctt
     6781 ttgttcatgg gctgctgcag gacaagcggc gggtatatga gcatctggcg gattatctgg
     6841 atgtggtcaa gtttgcggta aatatctccg atgtatggaa ttttctgtgg gatggatatc
     6901 actgccatct ccccgcctgc cgtcacctgc cggtaaacca tcggatgttt gccggtgatg
     6961 ttttcctgaa tgtgcctgta ggttgtcctg acaagatgag tcagatgttc aattgtttca
     7021 ttaaaggttc tgtaaggcct tttgtcgagt ctgtcacctt ctgaatcatt tatgatgaac
     7081 ctgtcgaaat ggcgccagaa atcatacagg tattccacaa aactgttaag cagaagcctg
     7141 tcgcggataa attctgccga gtcttctgaa atatgtggaa gaatttcgca cttatacttt
     7201 tcaagcagaa taagcgtcct gataagtcgg tcaattcccc tgtcagtcac cttactgtct
     7261 tcaaagattc ccagaagtgg agaattgttc ttttcaagct tccgcaggca aagttccagg
     7321 acagtcctga atttactgct ggtgagcagt tcctcaggtg tttcgcacat cctctcgcgg
     7381 atgtacagga tagctttgtt tccttctatt cttaatgtac ttctttccat accataaaaa
     7441 catggcaaaa atatcacaaa ttattataag tttcctggtt ctgttaaaat atttttgaag
     7501 aaaaaataaa ataatattaa aatgttatat tttaacattt tattgatata aatgttaatc
     7561 atttaacaga aaattttttc aaaatttaac ttaacatact tattttttcg ttgacggttt
     7621 tacagtgagc gttgcatgtc aaaaaagcag tacagttaaa tggctgaaaa agaagaaata
     7681 ataaaaattt gttaaaacta ttattcataa ataacatttt ggcatttttt gtgttatttg
     7741 aataatggtt aatattaatt ataaatttgc ataagaaggc attgcagagg attatgaagg
     7801 acgattttga ttttttcagc atattgccgg aggacaaaat accggaaaaa gggtgtgttc
     7861 ttatttcaga gcctttttta cctgactcat tctttaaccg gacaattgtt tttttgaccg
     7921 agcataatga agacgggtcg gtggggttta ttctaaataa gaaactggat ctccgggtat
     7981 gcgatgctat tgagggtttc gacaattgtg aggattatct cagcatggga ggtcctgtag
     8041 cacctgacac gcttcattat attcatacac tgggtgatgc aattcctaaa agtgtgcatg
     8101 tttataataa catctactgg agtggtgacc ttgaagccat ccgtaagatg tacagtttca
     8161 acaagattaa agatcatcag ataaggtttt tcctcggata ttcgggctgg gcagcaggtc
     8221 agctgaaaag ggaactgaaa gaaaattcat gggtaatagc aaggataagt ccggaagtta
     8281 ttatgaccag
//
LOCUS       DAOSNB010000084         8169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8746_length_8169_cov_4.866034, whole genome shotgun sequence.
ACCESSION   DAOSNB010000084 DAOSNB010000000
VERSION     DAOSNB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8746_length_8169_cov_4.866034"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PLG42_04895"
     CDS             complement(<1..330)
                     /locus_tag="PLG42_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30583.1"
                     /translation="MKRALIILLITLFATAVTEGVTQKEVSGENDKNTPIPYSQETKV
                     VIGDSLLSIQDNNEAFRLKIGNRGIEILESLEGKSRIKFEKYDSREDNKEMKSASSFC
                     NTCSITRR"
     gene            complement(381..1454)
                     /locus_tag="PLG42_04900"
     CDS             complement(381..1454)
                     /locus_tag="PLG42_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30584.1"
                     /translation="MKPERINYEIWILDYLDGRLNEEQTCLLMQFLDENPEIREELSF
                     LAEFNTIPEDTKYPFKNKLKKSITDLPEDQFELLCVSALENDISSEEAAELMQYLESR
                     SEKRNTFNQIQKIKLHPPVVEFRNKNKLRKRGIAEKTIRFLVPVLSAAACLFIAIWIF
                     TSDEINNKNNNNITLSDEEIQQKNPDKIATPEIVTPYTTKTYTTETKQNRFPRKNITP
                     VMPIPSPDLDENSRQEIIIPSPLAFKDNLVLSRIAIEKMMPDVKFNSTTAINDEETDQ
                     GLKYYFTKFVREKILRTNSGREDAIKSYEIAEASIKGLNKILGWQMSFKEYTDEKGEL
                     LAINFNSKLIKINIPVKRNEASE"
     gene            complement(1458..1943)
                     /locus_tag="PLG42_04905"
     CDS             complement(1458..1943)
                     /locus_tag="PLG42_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU30585.1"
                     /translation="MTVKEYNKAVDDFSDALYRFVRSSLKDDDRANDIVQDTYEKLWR
                     HVTEIEFKVVKAWLFSTAYNSMIDIIRKEKRTIPLEEEHENEITYESEYTDLNEILHH
                     ALDRLPEYQKIPVLLRDYEGYSYREIGEITGQTEAQVKINIYRGRIALKSFIGKIETV
                     L"
     gene            complement(2051..3466)
                     /locus_tag="PLG42_04910"
     CDS             complement(2051..3466)
                     /locus_tag="PLG42_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOU30586.1"
                     /translation="MASLKLLLGLIPSTEKIEQEEKTLLAEFEKLKSISESQKLARFN
                     ELHELVNSEGFKQKKKEIESLSYKNSEEYAKEKEFLSLKKAKDIVMYFRTVEGNALKR
                     FRELDGSEKIKKYEELKQLVSEAGFRERMKSKDFSGSDDARKLAEFKQMDSSPDIKEY
                     YSFKNSKEYANFLNIDGSSRLARFNELKEYTSSKEFQEKKAFLLDKNRFQKTEEYKTL
                     KEYLDLKNDPDIVWYLKTKDSDKFDVLKKRELVFSEEFDSDTLDAKKWLTNYYWGEKL
                     LHDRYSLESDLHFYTEKDNFEIRHSYLKIITKPQKIQGKAWSPVRGFTIKEFNYTSGI
                     INTGNSFRQKYGIFTAKIKLGDPNARHSFWLVGEKTTPHINICTTAGGKVWFTLFTED
                     GKQIKSKVSGKYAQKSYIFTLEWTPDKLIWKINGVEAFSTTENIPQEPMYINLAGGVT
                     APVNSMTSMEIDWVRVYIPKN"
     gene            3785..5092
                     /locus_tag="PLG42_04915"
     CDS             3785..5092
                     /locus_tag="PLG42_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30587.1"
                     /translation="MKSNRRTFLKTASVAAAASVIPFEAFSIGRPGVSPSDRIRVALI
                     GGNSMGWNDLASFLKNKEVECVAICDVDRNVLNRRTDDIVKLGFQKPRLYVDYRKMLE
                     SKDIDVVIIGTPDHWHCLMLVDSLEAGKHVYVEKPIGKSIAEINVMQKAVKKHGKMVQ
                     VGQWQRSQPHFVDAINFLKSGKLGRIRVCKAWSFVDWKGAVPKVPDSPVPEGVDYDRW
                     LGPAPKRPFNQNRFHQSWRWYWDYAGGLMTDWGVHLIDYILYGMNKAVPKSVMAIGGK
                     YAFPDDAMETPDTMTAVYDFEDFMMIWEHTIGIGLGNWRRPHGMAYTGENGTLVLDRN
                     GWEVIPDKGRMEAVPLQKNVGSGLDLHVRNFLDCLENNTPQKLNASIDVGRDVALVAH
                     MGNIAFLTGEKVYWDNAKQQFTSAAANKLITPVYYNGYKLPKY"
     gene            complement(5107..6081)
                     /locus_tag="PLG42_04920"
     CDS             complement(5107..6081)
                     /locus_tag="PLG42_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOU30588.1"
                     /translation="MKKILGIGNALVDIVTFIDDEKILEKFSLPKGSMQLVDKTTSLK
                     IREATKKFQSTFAPGGSAANTIYGLAMLGVKTGFLGATGKDETGKIFDSELHKAGVKT
                     FIKKLNNPTGTAISLVTPDSERTFATYLGAAVELSVDDLKKELFSEYDILYLEGYLIY
                     NKDFVVKACSLAKSLKMKVALDLASYNVVNDNIDAFKEVIVNYTDILFANSEEARAYT
                     GKEPEESLKDLSQKCEIAVIKTGANGSLIQSGNKIIKVQPFRVECRDTTGAGDLYAAG
                     FLYGLASGKSLEKCGQYGSYLAAKVIEITGARIESEKWPAIKKTISVL"
     gene            6161..6234
                     /locus_tag="PLG42_04925"
     tRNA            6161..6234
                     /locus_tag="PLG42_04925"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6195..6197,aa:Asn,seq:gtt)
     gene            complement(6196..7257)
                     /locus_tag="PLG42_04930"
     CDS             complement(6196..7257)
                     /locus_tag="PLG42_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU30589.1"
                     /translation="MHKKTQITIKDIAKELGISPSTVSKALKDHSDISDETKKAVREL
                     VEKLNYRPDPIALSLKIGQSKTIGVIIPEIVHYFFSTVISGIEDEAYNSGYQVIFCQS
                     NESYDREVRAVETLLSGRVDGILVSMSKTTDKYDHFKKIKEYGIPLVFFDRICEELET
                     DKVIVDDEEGAFCAVSHLINEGCREIVHLAGPQNLKIAQERMKGYIRAILKAGLTIKE
                     ENIIKCDTVNDARLIVPGLIKRENRPDGIFAVNDLTAAEAMKVIKASGLNIPEDISVV
                     GFTSGMISDLTDPPLTSVEQHGYEIGREAAKLLIGRIENKISGEFITRIIKTELIIKS
                     SCKFQTSLPRQGSNLRPTD"
     gene            7549..>8169
                     /locus_tag="PLG42_04935"
     CDS             7549..>8169
                     /locus_tag="PLG42_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU30590.1"
                     /translation="MKKSIILFLWMFFLGVFFTGTLYAQGIRVTGKVTDASDGSPLTG
                     VTIIEKGTSNGTMTDASGNYAITVSPTSVLQFSYVGYLSQEIPVNNRTTIDVSLAVDV
                     QALQEVVVIGYGTVTKKDATGSVVAIGTRDFNMGSVARPQELIIGKIPGVQITTSGGD
                     PTAGATIRIRGGSSLSASNDPLIVIDGVPIDNAGVSGMPNPLSLINS"
BASE COUNT         2316 a         1461 c         1535 g         2857 t
ORIGIN      
        1 ccgccgcgtt atggaacagg tattgcagaa cgatgaagca gatttcattt ctttgttatc
       61 ttctcgagaa tcatattttt caaatttaat acgtgactta ccttcaagag attcaagtat
      121 ttcaataccc ctgttgccga ttttcagtct gaatgcttca ttattatcct gaatacttaa
      181 caggctatca ccaataacta cttttgtttc ctgagaatac ggtatcggtg tgttcttatc
      241 attttcccca gaaacttctt tctgagttac tccttccgta actgcagttg cgaataatgt
      301 aattaataat attatcaatg ctcttttcat tttaaagttt tttaaatttc aaatatgaga
      361 cgtttaatgt atgttaaaag ttactcggaa gcttcatttc tctttaccgg aatattaatt
      421 tttatgagtt tggaattgaa atttattgcc aataattcac ctttttcatc ggtgtattct
      481 ttaaagctca tctgccaacc caggatttta ttaagccctt ttatactggc ttctgcaatt
      541 tcataagatt taatggcatc ctcccggcca gaattggttc taagaatctt ttccctgaca
      601 aattttgtaa aataatattt gagaccctga tcagtttctt catcatttat agcagttgtg
      661 ctattgaatt ttacatcagg catcattttt tcaatagcga tccgggatag caccagatta
      721 tccttaaatg ctaaaggtga tggaattatg atttcttgtc tgctattttc atctaaatca
      781 ggtgatggaa taggcattac cggtgtgatg tttttcctgg gaaatctatt ctgtttggtt
      841 tccgtagtgt aagttttagt tgtgtaagga gttactattt ctggagttgc aattttatca
      901 ggattttttt gttgtatttc ttcatctgag agagtgatat tgttattgtt tttattattg
      961 atttcatccg aagtgaaaat ccatattgca ataaaaaggc aggctgccgc actgagaaca
     1021 ggaaccagaa aacgaattgt tttttcagca atacctcgtt tgcggagttt gtttttatta
     1081 cggaactcaa caacaggcgg atggagcttt attttctgta tttgattaaa tgtatttctt
     1141 ttttcagaac ggctttcaag gtattgcata agttctgcag cctcttcgga tgaaatgtca
     1201 ttttcaagtg cactgacaca taatagttcg aactgatctt caggcagatc tgtgatgctc
     1261 tttttaagtt tgtttttgaa aggatacttt gtgtcttcag gtatagtatt aaattctgca
     1321 agaaaactta gttcctctct gatttcagga ttttcgtcaa gaaattgcat taacaggcag
     1381 gtctgttcct cgttaaggcg gccatcgagg taatcaagga tccatatttc ataattgatt
     1441 ctttcgggct tcatttgtca aagcactgtt tcaattttcc cgataaagct cttcaaagca
     1501 atccggcccc tgtaaatatt tatttttacc tgagcttcgg tctgtcctgt aatttctcca
     1561 atttccctgt aactgtatcc ttcataatca cgtagcaaaa caggtatttt ctgatattca
     1621 ggcagcctgt cgagtgcatg gtggagaatt tcgttgaggt cggtatattc tgactcgtaa
     1681 gtaatttcat tttcatgctc ctcttcaagt ggaatagttc ttttttcttt acgtatgata
     1741 tcaatcatac tgttgtatgc tgttgaaaag agccatgctt ttaccacttt gaattctatt
     1801 tcagttacgt gccgccagag tttttcgtat gtatcctgta caatgtcatt tgccctgtca
     1861 tcatctttca ggctgcttct gacaaaacgg tataacgcat cagaaaaatc atcaactgct
     1921 ttgttgtatt cttttacagt cataaaagtt ttaacactaa taaagacgga taaaagtgct
     1981 gaaagttaca tccagataaa aaaaaagagg ttatcattaa gacaacctcc attttaaatt
     2041 attatggaat tcaattcttt ggtatgtaaa ctcttaccca gtctatttcc attgatgtca
     2101 tactgttaac cggagcagtt accccacctg caagattgat atacattggt tcctggggaa
     2161 tgttttctgt tgtactgaaa gcttcaacac cattaatttt ccatataagt ttatcaggag
     2221 tccattctag agtaaagatg tagctctttt gtgcatattt tccactcact tttgatttaa
     2281 tctgttttcc gtcttcagta aagagtgtga accatacttt tcctcctgca gtggtgcaga
     2341 tattgatgtg tggagtcgtt ttttctccaa caagccagaa tgaatgtcgg gcatttgggt
     2401 caccaagttt tatttttgca gtaaagattc cgtatttctg cctgaacgaa tttccggtat
     2461 taattattcc tgatgtataa ttgaattctt taattgtaaa tccccgcaca ggcgaccagg
     2521 ctttgccttg aattttttgc ggctttgtaa ttattttcag gtagctatga cgaatttcaa
     2581 aattatcttt ttcagtataa aaatgaaggt cagattcaag agaatatcgg tcgtgcagga
     2641 gtttttcgcc ccagtaataa tttgtcagcc atttttttgc gtcgagcgtg tcactgtcaa
     2701 actcttcact gaaaacaagt tctctttttt tcaggacatc aaatttgtcc gaatcttttg
     2761 tttttaaata ccatacaata tcggggtcat ttttaaggtc gagatactct ttaagtgttt
     2821 tatattcttc agttttctga aatctgtttt tgtcaagaag aaatgccttt ttttcctgga
     2881 attcttttga acttgtgtat tcttttaatt cattgaatct ggcaagcctc gatgagccat
     2941 caatattcaa gaaatttgca tattcttttg aatttttaaa actgtaatac tcttttatat
     3001 ccggggatga atccatctgt ttaaattctg ccagttttcg ggcatcatcc gaacctgaga
     3061 aatcttttga tttcattctt tctctgaatc ctgcttcaga tacaagctgt tttagttctt
     3121 cgtatttttt tatcttttca gaaccgtcaa gttccctgaa tcttttaagc gcgtttcctt
     3181 cgacagtcct gaagtacata acaatatctt tggctttttt caaagaaaga aattcttttt
     3241 ctttagcata ttcttcagag tttttatatg aaagactttc aatttctttc tttttctgtt
     3301 taaaaccttc tgaattgaca agttcatgca attcattgaa ccttgcaagc ttttgtgatt
     3361 cagaaattga ttttagcttt tcaaactctg ctagaagtgt tttttcttcc tgctcaattt
     3421 tttcggttga gggaataagg cctagtaaaa gctttaaact tgccatgagt ttataaattt
     3481 tgatataaca tgctaaagat aaaaaaactt ttattaatta atgattaacc atttgaatga
     3541 ttatatacaa caatttaatt taatcatgta aagttatgat taaaaaaact aaatataaca
     3601 aattacagaa ataaaattgc ttcatttaaa aatatataac taaaaacgaa atctgtaata
     3661 cattgtttta tagacgttaa aaaaaattat aaagtacctc ttctaagtaa catgaaaaaa
     3721 taaaagtctg gcttattcgt ttattttttt aaatttgcac tcattattta atacagttag
     3781 atttatgaaa tcgaatagaa gaactttctt aaagactgca tcagttgctg ctgccgcgtc
     3841 ggttatacct tttgaagcgt tttccatagg caggcccggt gtatctccga gtgacagaat
     3901 aagagtggca ttaataggtg gaaatagtat gggctggaac gatttagcct ctttcctgaa
     3961 gaacaaggag gttgagtgtg ttgccatatg tgatgttgac cgaaatgttc ttaacaggcg
     4021 aacagatgac attgttaaat tgggattcca gaaacccagg ttatatgtag attacagaaa
     4081 gatgcttgaa agcaaggata ttgatgtagt cattattggt acgcccgacc actggcattg
     4141 tcttatgctg gttgattcac tagaagctgg caagcatgtg tatgtagaaa aaccgattgg
     4201 caaatcgatt gccgagataa atgttatgca gaaggctgta aagaaacatg gaaagatggt
     4261 gcaggtaggg cagtggcaga ggagtcagcc ccattttgtt gatgcaatta attttcttaa
     4321 gtccggaaaa ctggggcgta tcagggtttg caaagcctgg tcgtttgttg actggaaggg
     4381 agctgtacca aaagtaccgg attctccagt accagaaggt gttgattatg accggtggct
     4441 gggacctgct cctaaaaggc cgttcaatca aaacagattt caccagtcat ggaggtggta
     4501 ctgggattat gcaggtggat taatgactga ctggggtgtg caccttattg attatatact
     4561 ttatggaatg aataaggctg ttcccaaatc agttatggct ataggtggta aatatgcttt
     4621 ccctgacgat gcaatggaga cacctgatac aatgacagcg gtatatgatt ttgaagactt
     4681 tatgatgatc tgggaacata ccatagggat cggacttgga aactggagaa gacctcatgg
     4741 gatggcttat acaggtgaaa atggaacact tgttcttgac cgtaacggct gggaagtgat
     4801 tccagacaaa gggagaatgg aagcagtacc tttacagaaa aatgttggtt ctggactgga
     4861 ccttcatgta agaaattttc ttgactgcct tgagaataat accccgcaaa agcttaatgc
     4921 cagcatagat gtaggaagag atgtggcatt ggttgcccac atgggaaata tcgctttcct
     4981 gaccggcgaa aaggtatact gggataatgc aaaacagcaa tttacgtcag ctgctgccaa
     5041 taaacttatt actccggtat attataacgg atataagttg ccaaaatact aatctgctgt
     5101 atccagttat agcactgaaa tagttttctt tattgcgggc catttttcgg attcaatacg
     5161 tgcccctgtt atttctataa cttttgctgc aaggtaggag ccatattgtc cgcatttttc
     5221 aagggatttg ccggatgcca atccataaag aaaaccagca gcataaagat cacctgcacc
     5281 agtagtatcc ctgcattcaa cccgaaaagg ttgtacctta ataattttgt taccactttg
     5341 tataagagaa ccattagctc ctgtttttat aacggcaatt tcacattttt gggataagtc
     5401 cttaagtgat tcttccggtt ctttacctgt atatgctctg gcttcttcgg agttggcaaa
     5461 caaaatatca gtataattga ctattacctc tttaaaagca tctatattgt catttaccac
     5521 attgtaactt gccaggtcaa gtgcaacttt cattttaagt gatttagcca gagaacaggc
     5581 tttgaccaca aaatctttat tgtatataag atatccttcg aggtatagaa tatcatattc
     5641 tgaaaacagt tcttttttca ggtcgtcaac agacaactct acggctgcgc cgagatatgt
     5701 ggcaaaagtt ctttcactat cgggagtgac aagagatata gctgtgcctg tagggttgtt
     5761 cagttttttg atgaaggttt ttacacctgc tttatggagt tcagaatcaa atattttccc
     5821 tgtttcgtct ttgcctgtag ctccaagaaa gccggttttt actccaagca ttgccaggcc
     5881 gtaaatggta tttgctgcag atcccccagg tgcaaacgtg ctctgaaatt ttttagttgc
     5941 ttctcttatt ttcagcgaag tggttttgtc tacgagttgc atacttcctt taggcaggga
     6001 aaatttttca agtatttttt catcgtctat aaatgttacg atgtcaacca gagcgttacc
     6061 tattccaaga atttttttca taaaaatttt ttcaaagata ttgttaaatt cactaaaagc
     6121 ctgtaatttt gccacgcaaa aaatttgatg cggtaaaaat tcctcagtag ctcagctggt
     6181 tagagcggcg gactgttaat ccgtaggtcg caggttcgat ccctgcctgg ggagcgaagt
     6241 ttgaaactta catgatgatt ttattataag ttctgtcttg ataatcctgg taataaattc
     6301 tcctgagatt ttattttcta tcctgccgat cagtaatttt gctgcttctc ttccgatttc
     6361 atagccatgt tgttcaacag aagtaagcgg agggtcagta aggtcagaaa tcatgccact
     6421 ggtgaagcct acaacagaaa tatcttcagg gatatttaac cccgatgctt taataacttt
     6481 catagcttca gctgctgtta ggtcgttaac ggcaaaaata ccgtctggcc tattttctct
     6541 tttaataagt ccgggcacaa ttaatcttgc atcattgaca gtatcgcatt tgataatatt
     6601 ttcttctttt attgtaagcc cagcttttaa tatagccctt atgtatcctt tcattctttc
     6661 ctgggcgatt ttcagattct ggggtccggc cagatgtaca atttccctgc aaccttcatt
     6721 aatcaggtgg ctgactgcgc aaaaagcccc ttcttcatca tcaacaatta ccttatcggt
     6781 ttccagctct tcacatatcc tgtcaaaaaa tacaagggga attccatatt ctttaatttt
     6841 cttaaaatgg tcgtatttgt cagtagtttt tgacattgag acaagtatcc cgtcgaccct
     6901 tcctgagaga agcgtttcta ctgcacgtac ctccctatcg tatgattcgt tcgattggca
     6961 aaaaattacc tgataaccag aattataagc ttcatcttca ataccgctga taacggttga
     7021 aaaaaaataa tgaacaattt ccggaataat aacaccgata gttttactct gcccaatttt
     7081 aaggcttagg gctatgggat caggcctgta attaagcttt tcaaccagtt cccttacagc
     7141 tttttttgtt tcatctgaaa tatcggaatg atccttcaat gcttttgaaa cggttgaggg
     7201 tgatatacca agctcttttg ctatatcttt gatggttatc tgtgtctttt tatgcatact
     7261 ctctgataag cgtcataaat ttacatttta ttatctctct tttttcaaac ttttaaactt
     7321 aaaattattg actataaaca gaacatatat tttaaaaaat caacagtcgg gtagttttaa
     7381 aataatcttg tttacatttc aaacgtttgc ggtaaaaagt tcttaattta ttgaaaatta
     7441 atgtatttat attgttttta aattgttttt tttataaatt tgttacattg taaaatcatt
     7501 attgaatttt caggacataa atagtaacta atttaaaact aattttttat gaagaaaagt
     7561 atcatattat ttctgtggat gtttttcctg ggggtgttct ttacagggac actttatgcg
     7621 cagggaataa gagtcacagg taaagttaca gatgcttcgg atggatcccc tctaacaggt
     7681 gtaacgatta ttgaaaaggg gacatctaac ggcaccatga ctgatgccag tgggaattat
     7741 gcaattactg tatctccaac atcagttcta cagttttcat atgtgggata tctgagtcag
     7801 gaaataccgg taaataacag aacaacgatt gatgtttcac ttgcagttga tgtgcaggca
     7861 ttgcaggaag tagttgtcat tggttatggt acagttacca aaaaagatgc aacaggttcg
     7921 gttgttgcaa tagggacacg tgattttaac atgggttcgg ttgcaaggcc tcaggaactt
     7981 attataggta agattcctgg tgtacagatt acaacatcag gtggggatcc tactgccggg
     8041 gcgacgataa gaataagggg tggctcgtcc ctgtcggcaa gcaatgatcc tcttattgtt
     8101 attgacggtg ttccgataga caatgctggc gtttcaggaa tgccaaaccc attaagtctt
     8161 atcaactca
//
LOCUS       DAOSNB010000085         8039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8949_length_8039_cov_5.333166, whole genome shotgun sequence.
ACCESSION   DAOSNB010000085 DAOSNB010000000
VERSION     DAOSNB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8949_length_8039_cov_5.333166"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..409)
                     /locus_tag="PLG42_04940"
     CDS             complement(143..409)
                     /locus_tag="PLG42_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30591.1"
                     /translation="MVRPLFGNYFLLVPPGRGLTEHCPGHRADACGPFEFALGALVNA
                     SRYPPSYRHLGEDFLLIISWEKLFAVISGLFIKEKSLFTGHPAS"
     gene            563..994
                     /locus_tag="PLG42_04945"
     CDS             563..994
                     /locus_tag="PLG42_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOU30592.1"
                     /translation="MADVLTPEQRKLNMSKIRGKNTTPEKVIRNKLYSLGIRGYRIHY
                     NLPGKPDIVFVRKRIVVFIDGCFWHKCPLDFQEPETRKEFWLNKIEKNVIRDRKVDLE
                     LSKKGWTVLRIWEHEVKKDPDLVVHKIMSLLEKTAVSDNNF"
     gene            complement(991..1989)
                     /locus_tag="PLG42_04950"
     CDS             complement(991..1989)
                     /locus_tag="PLG42_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK motif protein"
                     /protein_id="HOU30593.1"
                     /translation="MIRIKPDVWQYLENSHPQGQKLNARQALPEISPRLLCALDSENT
                     RHLLIPLNETEAGLNDRQTRGLYVITRELQVHTEMPRSYIDIVCRDSAGYDAFDLIAG
                     DIASNLALNRKPADSVRSVLAKWRRFWGQMPLDILSRPEIIGLFSEIWFISQWLCQRI
                     EPIEILKHWRGPFGARHDFEWQGRSIEVKATTSNRGRIFHINGLEQLEPPENGNLFLF
                     GLILREESGASHTLASLIADFRTKIKDDGLLQSFFDEALVESGYSPAHDEHYERYRFR
                     VVEEVLFSVSGNFPKIIRINFVNGSLSGIERVEYDINLSGFDHLIVARSPEEGDWI"
     gene            complement(1976..4618)
                     /locus_tag="PLG42_04955"
     CDS             complement(1976..4618)
                     /locus_tag="PLG42_04955"
                     /inference="COORDINATES: protein motif:HMM:NF022063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HOU30594.1"
                     /translation="MNFEEALGIVRMLIETGIPRESAINNPAVPVEFRSEIENRLRGE
                     EIIVLEPADEISSSVQSDEWLNKIDRSNWYYWPTLRGYFLSVKKFPLYTVRSIDESTD
                     RILGRMPNPSTSRFDVRGLVLGYVQSGKTANYTALIAKAADVGYRLIVVLSGIDNGLR
                     RQTQIRLKKELVGYADHQKAKSVPLPPLGRQWHEFTTEDIDGDFRPGFASPAALQGSQ
                     PVLLVIKKNGTVLRRLLNWLDNAPEEVKHTIPLLVIDDEADQASIDTRGTYIHGETPL
                     PDDYEPPSVINSLIRQLLNKFHRSSYVAYTATPFANILIPHDANDPEYKNDLFPKNFI
                     FALPKPHGYFGAEELFGISNSDEEEPRGLDIVREVSPDDLQNLEQNQLPVSLKNAIID
                     FILAGAVRAARGEPEKPATMLVHISRLIAEQNNLAEIIGQYLQELRDEWRYFRNNGIL
                     EQLRSRWEHEFRPVTKNICPDRDMDFEKIEPYIGHFFESVKLKIINSATGDVLDYERE
                     PQLKAIAIGGDRLSRGLTLEGLLVSYFVRRTPMYDTLMQMGRWFGFRSGFEDITRIYT
                     TAELAGWFSDLAVVENQLRDDIKIYENLGLTPYEVGTRILLHPSLQVTSPLKMKYTRQ
                     ITIRQSFSGEKIQTFKFPLNHPDKLVLQEENNLKGIRKFLLQLGNPAAISDRKGPIWT
                     NVPADLILECLRSFCVDIESGMFTPDLICKYIEQQNEKCELLRWTVAIRGRELPDPVL
                     GDVDWGIPGMTVHQISRTRLGNTENLGVITSPGDEAIGLSDSSRLIMQQLLNEGYKQD
                     PAARRARPPEEALLLIYPISKYSGHERRSSGGNRRPLYDDPNQPHARNLIGLAISFPH
                     SNNVQPVTAYLTGTAGWRLSIDQD"
     gene            complement(4638..5972)
                     /locus_tag="PLG42_04960"
     CDS             complement(4638..5972)
                     /locus_tag="PLG42_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU30595.1"
                     /translation="MGYDFPTAVADLIDNSIDAGATEIHITVQFDGDKSWVSIADNGK
                     GMSGDEIKEALRFGSETEYNMNNLGKFGLGLKTASLSQCQVLSVASRADPEKTQISAY
                     CWDLNHIEKVNRWEILPIKTDEMHHVLRDPLLTSTGTVVMWQKLDRILGYKNPYGEFA
                     KKRMAAMCRELEEHLAMVFHRFIMGESRRQTIKIFLNNNLIRPWDPFARSEAKTWILE
                     STEIPCELEDIKGTIYIQPYILPHKDEFSTRDAWEYASGPKKWNQQQGLYIYRGDRLI
                     QSGGWSYLRTIDEHTKLARIAISFSPDLDEAFKINVAKMRVQLPIEIQEKLKQIIEPV
                     VRQARDRYDRPPVHSIISPINLPATTTRQNSNPAPNPVRITNPENFGCQTSLPLRKQI
                     HDIFPVSKTASTAEDRKWTLDEIERNLEKVAEPEEREIIVRVFAKLRQFLSK"
     gene            complement(6110..7393)
                     /locus_tag="PLG42_04965"
     CDS             complement(6110..7393)
                     /locus_tag="PLG42_04965"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOU30596.1"
                     /translation="MTESKTKNRYWVLDLFSGAGGLTEEFLRKNFDFVSHIEINHHAS
                     NTLETRILYYKLIKEKQKDLYYQYYNGEISREEFFLECKNIGLINNGIINQEISDNTR
                     DSIINNIKNHMKELENNLNVDVLIGGPPCQAYSLLGRSRDPNRMRTDPRNSLYLHYLK
                     FIQEFQPTFFIFENVPGMISAKNGEIYRDFISKINELGYTSDIKPRILDAQNFGVIQT
                     RKRIIVIGWKRDYDFSYPEFFENKPDVTVNDILRDLPPLNPGEGTDDLQHYRRIRPSK
                     YLSKYGIRTDEKGIRQHSARIHNERDREIYRIAINLWNTKRKRLHYDELPSELKTHRN
                     RTTFHDRFKVVDGNGKSHAIVAHLSKDGHFFIHPDIKQARSLTVREAARIQSFPDNYL
                     FEGPRTSQYVQVGNAVPPLMAEGIAVEIEKMLAEI"
     gene            complement(7613..7933)
                     /locus_tag="PLG42_04970"
     CDS             complement(7613..7933)
                     /locus_tag="PLG42_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30597.1"
                     /translation="MPGSGDYTRFALNDAESNVNDEKNVPVIEIEKAENLCSVGGVVC
                     IRTGTRLRLRSVEEILILSPGTGAVAWDESQFIPKNGRRAVYSVIPEKIEKSVTPDHN
                     RGKG"
BASE COUNT         2175 a         1763 c         1597 g         2504 t
ORIGIN      
        1 ccgtgccgca tgccttttac gagatcgagt acggtctcgt gaagggaggg gggagggggt
       61 aaccttccct ctctatagaa tcaccgggac tctgggtgaa gaattctgtc tgcttcgaat
      121 gggtgaggat cctttttttg aatcaggatg ccggatgacc ggtgaacaag gatttctctt
      181 taataaatag ccctgaaatc actgcaaaga gtttttccca ggagataatc aggagaaagt
      241 cctctcccag gtgcctatac gagggcggat aacggctggc attgaccagt gcaccgagtg
      301 caaactcgaa agggccgcat gcatcggccc ggtgaccggg acaatgttct gtaagtcctc
      361 ttccgggagg caccaagaga aagtaattgc caaacagagg ccgtaccatg caaagagccc
      421 cgaaaaaatg aaggagaaga atttctggta ctacaggata cagaaggaga aggcagaggt
      481 aacggtaaga gagaagggtg tttgcggggt gaggggggcg gatagagatt aattaataac
      541 gattaatcca attattttta ttatggctga tgtcctcacc ccggaacagc gaaagctgaa
      601 tatgtcaaag atcaggggta agaacacgac accggagaag gttattcgga ataaattata
      661 ttccctgggt atcagaggtt atcggataca ctataatctt ccagggaagc ctgatattgt
      721 attcgtccgg aagcgcattg ttgtttttat tgacggatgt ttctggcata aatgtccact
      781 tgatttccag gagcccgaga caaggaagga attttggttg aataaaattg aaaagaatgt
      841 tatcagggac agaaaagtcg atttggaatt aagtaaaaaa ggatggacag ttcttcggat
      901 atgggaacat gaagtaaaga aggatcctga tctggtagtg cataaaatta tgtctctttt
      961 ggaaaaaact gctgtttctg ataacaattt ttaaatccag tccccttctt ccggagatcg
     1021 tgcaactatc agatggtcaa aaccggataa attgatatca tattcaactc tttcgattcc
     1081 actcaatgaa ccgttgacaa aattaatcct gataattttt ggaaaattac cgctgacaga
     1141 aaacaaaact tcttctacta cccgaaaccg gtatctctca taatgttcat catgtgcagg
     1201 agaataaccc gattcaacca atgcctcatc gaaaaaagac tgtaatagtc cgtcatcttt
     1261 tattttcgtc ctgaaatccg ctatcaaaga tgcaagagtg tgactggcac cggattcttc
     1321 ccttaatatt aatccgaaaa gaaagagatt cccgttttcg gggggttcga gttgttccag
     1381 accattgata tggaatattc ttccccggtt tgaagttgtt gctttcactt caattgatcg
     1441 accctgccat tcaaaatcat gacgtgcacc aaacggccct ctccagtgtt tcagaatttc
     1501 aatcggttca atcctttgac aaagccattg cgatataaac catatttcgg aaaataatcc
     1561 tattatttcc ggtctggaaa gaatatcaag aggcatttgt ccccagaatc ttctccattt
     1621 tgcaagaacg gagcggacac tgtcagcagg cttgcgatta agggcgagat tcgaagcaat
     1681 atcccccgct atcagatcaa atgcatcata tcccgcagaa tcccgacaga cgatatcaat
     1741 ataggagcgt ggcatttcag tatgaacctg taattctctg gttattacat acaggccccg
     1801 ggtttgtctg tcgtttaacc cggcttctgt ttcattaagg ggaatcaaca aatgacgtgt
     1861 attttccgaa tccaaagcac aaagtaaacg cggggaaatt tccggcaggg cctgtctggc
     1921 attgagtttt tgaccctggg gatgactatt ctccagatat tgccaaacat ccggcttaat
     1981 cctgatcaat gctgagcctc catcctgctg tccctgtcag ataggcagtt acaggctgca
     2041 cattattgga atgcgggaaa gatattgcaa gaccaatcag atttctggca tggggttgat
     2101 tcggatcgtc atataatggt cttcgattcc ctcctgaaga tcttctttca tgacctgaat
     2161 atttactaat tggatatatg agcaacaagg cttcctcagg gggtctcgct cttcttgcag
     2221 ccggatcctg tttatatcct tcattgagaa gctgttgcat aatgagccgc gaactgtcag
     2281 acaatccgat tgcctcgtct ccggggcttg tgatgacacc gagattttct gtgttcccaa
     2341 gtctcgtacg actgatttga tgaacagtca ttccggggat gccccagtca acatccccaa
     2401 gtacgggatc aggaagttcc cggcctctga ttgctacagt ccaccgcaat aattcacatt
     2461 tttcattctg ctgttcaata tatttacaaa tcaaatccgg tgtgaacata ccggattcaa
     2521 tgtcaacaca aaatgatctg agacattcga gtatcagatc tgcaggtaca tttgtccaaa
     2581 tcggaccttt cctgtcggaa attgcggcag gattccccaa ctgcagcaga aactttctga
     2641 ttccttttaa attattttct tcctggagca cgagcttatc cggatgattc agtggaaatt
     2701 tgaaagtctg aattttttct ccggaaaaac tctgccggat tgtgatttgc cgggtatact
     2761 tcattttgag agggcttgtt acctgcaggg atggatggag caaaatcctc gttccaactt
     2821 catacggcgt taaaccaaga ttctcgtaga ttttgatatc atcacgcaat tgattttcaa
     2881 ccactgccag gtcactgaac catcctgcaa gctcggctgt agtgtaaata cgggtaatat
     2941 cttcaaatcc gctgcgaaat ccaaaccatc ttcccatctg cataagggta tcatacatcg
     3001 gtgttcttct gacaaaataa ctgactagta atccctccaa cgtcaatccc cgggataacc
     3061 ggtcgccacc gattgcaatt gccttcagtt ggggttctct ctcataatcc agaacatcac
     3121 cggtagcact gttaattatt ttcaatttta ctgattcaaa gaaatgaccg atatatggct
     3181 ctatcttttc aaaatccata tcgcgatcgg gacagatatt ttttgtaacc ggccggaatt
     3241 catgctccca tcttgaccgt aattgttcaa gaataccatt atttcggaaa tatctccact
     3301 catcccgcaa ttcctgcaaa tactgaccaa taatttctgc cagattattt tgttcagcaa
     3361 taagccggct gatgtgaaca agcattgttg ccggtttttc cggttcacct cttgcagctc
     3421 gtaccgctcc ggccaaaata aaatcaatta ttgcattttt cagagatacc ggaagctgat
     3481 tctgttcaag attttgtaaa tcatcaggag atacttccct gacaatatcc aatcctctgg
     3541 gttcttcctc gtcggaatta ctgataccaa aaagttcctc tgctccaaaa tatccgtgcg
     3601 gttttggcag agcgaaaata aaattttttg ggaataaatc atttttatac tcaggatcat
     3661 ttgcatcatg gggaatcaga atatttgcaa aaggtgtagc ggtataggca acataggaag
     3721 aacgatgaaa tttattcaat aattggcgga ttaaactgtt aattactgat gggggttcat
     3781 aatcgtctgg caatggtgtt tctccgtgta tataagttcc ccgggtatca atacttgcct
     3841 gatctgcctc atcgtcaata acaaggagcg gtatagtatg ttttacttct tccggcgcgt
     3901 tatcaagcca gttaaggaga cgtctgagaa cggttccatt ctttttaatc acaagcagaa
     3961 ccggttgtga accctgaagc gctgctggtg aggcaaaacc tggtctgaaa tccccatcta
     4021 tatcttctgt tgtgaactca tgccattggc gtccaagtgg cggcagggga actgatttcg
     4081 ccttttgatg atcagcataa cctaccagct ccttcttcag acgtatctgg gtttgtcttc
     4141 ttaagccgtt gtcaattcct gacaaaacaa caatgaggcg atagcctaca tccgcagcct
     4201 ttgcaatcag agcagtataa tttgctgtct taccactttg aacatatccc agaaccaatc
     4261 ctcttacatc aaaccgactg gttgatggat tgggcatccg acccaatatc cggtctgttg
     4321 attcatcgat ggatctgacg gtatataaag gaaatttctt aacacttaga aaatatcctc
     4381 tcaatgtggg ccagtagtac cagtttgatc gatcaatttt attcagccat tcatcggatt
     4441 gtacagaact gctgatttca tctgcaggtt ccaggacaat gatttcttcc cctcgtaacc
     4501 tgttttcaat ctctgaacga aactcaacag gaacagcagg attgttgata gctgattcac
     4561 gcggaattcc agtttctatc agcattctga cgattcccaa tgcctcttca aaattcataa
     4621 ataatcaccg tgatgtttca ttttgagaga aactgtctta gttttgcaaa gactctgaca
     4681 attatttctc tttcctcagg ctctgcaact ttttccaggt ttctttcgat ttcatcgagt
     4741 gtccactttc tgtcttctgc agttgaagct gtttttgata ccggaaaaat atcatgtatc
     4801 tgtttcctga gagggaggct ggtctgacaa ccaaaatttt caggattggt aatccggacc
     4861 gggttgggtg caggatttga attctgtctg gtagtggttg cgggtaaatt gatcggggaa
     4921 attatggaat gtacaggagg tctgtcatac ctgtcccggg cctgtctcac tacaggttca
     4981 ataatttgtt ttaacttttc ctgaatctcg atcggcagtt gaacacgcat ttttgccaca
     5041 tttattttaa atgcctcatc aaggtcaggt gaaaagctga tcgcaatacg agccagtttt
     5101 gtatgttcat caatcgttct taaataagac cagcccccgc tttgtatcaa ccggtctcca
     5161 cggtaaatgt acaatccctg ttgctgattc cacttttttg gtccgcttgc atattcccat
     5221 gcatcacgag tggaaaattc atctttatgg ggcagaatat aaggctgaat atagatcgtc
     5281 cctttgatat cttccagttc acacgggatt tctgtgcttt ccaatatcca tgtcttcgct
     5341 tcacttcgtg caaaaggatc ccacgggcgt attaaattat tatttaaaaa aattttgatt
     5401 gtttgtctcc ggctttcccc catgatgaat ctgtgaaata ccattgcaag gtgttcttca
     5461 agctcccggc acattgctgc catgcgtttt ttggcaaatt ctccatatgg gtttttatat
     5521 ccaagaatcc tgtccagttt ctgccacatt acgacagttc cagtagatgt taacaaagga
     5581 tctcgaagca catgatgcat ttcatcagtt tttattggaa gaatttccca tctgttcact
     5641 ttttcaatat ggttgagatc ccaacaataa gcagatattt gtgttttttc cggatcggcc
     5701 cggctggcta ccgataaaac ctgacattga cttaaagaag ctgtttttaa tcccagtccg
     5761 aattttccaa gattgttcat attgtattct gtttcggaac caaaccgtaa tgcctctttt
     5821 atttcatccc cggacatgcc ttttccattg tccgcaatgc tgacccagga tttatcacca
     5881 tcaaactgaa cagttatgtg aatttccgtt gcccctgcat caattgaatt gtcaatcaga
     5941 tctgcaacag ctgtgggaaa atcatacccc atatcccgca gagattttat caaacgttta
     6001 gctgatggaa ttaattcagt attttcattt tccgacatta tttcatctct tttcaatcag
     6061 ataattatat tttttaatat ttttatcctt tgaccagtca tttgttttat taaatttccg
     6121 ccagcatttt ttctatctcc acagctattc cttctgccat cagtggcgga accgcgtttc
     6181 ctacctgcac atactgtgaa gtccgcggtc cttcaaacag atagttatca ggaaatgact
     6241 gtattctcgc agcttctctg acagttaatg atcttgcctg tttaatatcc ggatgaatga
     6301 aaaagtgacc gtcctttgat aaatgggcta ctatggcatg tgattttccg ttaccatcaa
     6361 ctactttgaa acgatcgtga aaagttgttc tgttacggtg ggttttcagt tctgatggca
     6421 attcatcata atgcaggcgc tttctctttg tattccacag atttatggca atccggtaaa
     6481 tctcccggtc tctttcatta tggatcctgg cactgtgttg tcggattcct ttttcatctg
     6541 ttctgatacc atattttgaa agatatttgg agggacgtat tcgcctgtaa tgctgcagat
     6601 catctgttcc ttcaccagga ttaagagggg gaagatcacg aagaatatca tttacagtga
     6661 catccggttt attttcgaag aattcgggat atgaaaaatc atagtctctt ttccatccaa
     6721 tcacaattat acgctttctt gtttgaatca ctccaaaatt ttgagcatca agaattctgg
     6781 gtttaatatc agatgtatat cccaattcat taatctttga aataaaatcc cgatatattt
     6841 ctccgttttt ggcacttatc atacccggaa cattttcaaa tataaagaaa gtcggttgaa
     6901 attcctggat aaatttcaga taatgcagat aaagtgagtt ccggggatca gttctcatcc
     6961 tgttcggatc ccggctcctg ccaagaagtg aatatgcctg acagggcgga cctcctatca
     7021 aaacatcaac atttaaatta ttttccagtt ccttcatatg attctttata ttattgataa
     7081 tggaatcccg ggtattgtcg gaaatttcct gattaattat tccattattt attaatccga
     7141 tatttttgca ttccagaaaa aattcttccc gtgaaatctc tccattgtaa tattgataat
     7201 agagatcctt ttgtttttct tttatcagtt tataataaag aatccttgtt tccagtgtat
     7261 tactcgcatg gtgattgatt tcaatatgcg atacaaaatc aaagtttttt cttaagaatt
     7321 cctcagtcag gccaccggca cctgaaaaca aatccaaaac ccagtatcgg ttttttgttt
     7381 tggattcagt catctgatta ctccggaatg ttgttatttt cctgttatct acttaaaaat
     7441 tattttttat tacaggttat ctaactatag ccatgcaggg tgaaactgtt tccattggat
     7501 tccaatgctc catacatctt tttgtttaaa tgtcgaacgt ggattcaggc ttttcggagt
     7561 tttattcagg tgacaggcaa tggaaagtaa tcaaagtcat ttccaagatt ctctatcctt
     7621 tacctctgtt gtggtcagga gttacactct tttcaatctt ttcaggaatt actgaatata
     7681 ccgctcttct gccatttttt ggaatgaact gagattcatc ccaggcaaca gcccctgtcc
     7741 ctggcgaaag tatgaggatt tcctccacag accgcaggcg cagacgtgtc cctgtcctta
     7801 tgcaaacgac ccctccaaca gaacagaggt tttcagcctt ttcaatttct ataacaggca
     7861 cattcttttc atcgttaacg ttactctctg catcgttcag agcgaagcgg gtataatccc
     7921 ctgaacccgg catgcaaagg tatccgggtc aattcgggaa aaagatcaaa caatcccatg
     7981 ttcctcattt ccgctcctga atcgcaggct tctcatatat gcaccttctc tctcttccc
//
LOCUS       DAOSNB010000086         8036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8955_length_8036_cov_4.982960, whole genome shotgun sequence.
ACCESSION   DAOSNB010000086 DAOSNB010000000
VERSION     DAOSNB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8955_length_8036_cov_4.982960"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /gene="proB"
                     /locus_tag="PLG42_04975"
     CDS             <1..1095
                     /gene="proB"
                     /locus_tag="PLG42_04975"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOU30598.1"
                     /translation="RITVKVGSNVLTNDDGSLNTWRISRIVEDLAILYRNGIEVILVT
                     SGAVAAGRSEITPSRKTSVVESRQILAAVGQVKLMSHYQYLFGKYGIPAGQVLATRES
                     FMDRRHYLNMKNCIMAMLDNKIIPIVNENDTISVNELMFTDNDELSCLITSMMDCSSL
                     IILTNVDGIYTGIPGDDGTELIREVRPDAEGIEQYIFSFRSEFGRGGMLTKYSNARRL
                     ASEGVRVFIANGLRDSIITDIVRGKDVPYTHFLPAGKRRNSVKKWIAHSATFARGVVV
                     VNRGAKEALMGPKASSLLLVGVTRIEGSFKKGDIVRIIDEEGNSIGLGKSEYDSDKAI
                     QNIGQKCSKPLVHYHYLVLNENAGVEPVVF"
     gene            1117..2364
                     /locus_tag="PLG42_04980"
     CDS             1117..2364
                     /locus_tag="PLG42_04980"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOU30599.1"
                     /translation="MNCIPLLKRAAEAGRKMTVIDNSRINEVLLKLADAVEENIPFII
                     SENEKDLRKMDQNDPRYDRLLLTKERIEGIASDIRNVASLPSPVGRILDQRVRPNGLK
                     ISKVSVPFGVVAVIYEARPNVTLDVFSLCFKSGNACVLKGGSDAINSNKALVSIIAGI
                     LEKEGIDKNVVTLLPAGRSETMQLLKARDYIDLIIPRGGKSLIDFVRENALVPVIETG
                     AGICHTYFDEYGDREKGRLIINNAKTRRVSVCNALDCLIVHEKRLSDLPYLVSLCAEK
                     NVIIYADRKAFSALKGKYPAHLLQHATKESWGTEFLSYKMAVKTTSSFDEAISHISRY
                     SSKHSEAIISEDPDRIKMFFNLVDAAAVYSNTSTAFTDGAQFGLGAEIGISTQKLHAR
                     GPMALEELTTYKWIIEGNGQIRS"
     gene            2382..>4555
                     /locus_tag="PLG42_04985"
     CDS             2382..>4555
                     /locus_tag="PLG42_04985"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOU30600.1"
                     /translation="MYDFEIIKDFYEALPERMRKIRKRAGRPLTLTEKILFNHLYENG
                     AAGDLNRSGQYADFKPDRLAMQDATAQMALLQFILAGSKRSAIPASVHCDHLIVAEKG
                     GEEDLKKAVETGKEVYEFLESVCRKYGISFWKPGSGIIHQIVLENYAFPGGMITGTDS
                     HTPNAGGLGMIGVGVGGADAVDAMTGMPWELKMPEIIGVRLTGRLSGWCSPKDVILKL
                     AGILTVKGATGSVIEYFGDGTSTISCTGKATICNMGAETGATSSVFPFDESMVHYLNS
                     TGRNQVAELASKNRMHLQADPEVYAAPEKYYDRVIEINLSELEPHLNGPFTPDLATTV
                     QEMKKKAEKHNWPLDVEAVLIGSCTNSSFEDLSKAASVVKNALEKKLKPIAKIYVSPG
                     SEFVRTLAAREGILDLFRRAGAEIFANACGPCIGQWKRQTDENERVNTIVHTFNRNFS
                     KRTDGNPNTHVFVASPEIAAAIAFSGKLSFDPSRDKIVNEEGKLVMLDEPLAKELPEN
                     AAGLMSETVAVKNIQDEDTEIIIPPNSERLQLLKPFEPWNGKDFQGLYLLIKVKGKCT
                     TDHISMAGPWLKYRGHLENISENYMIGAVNAFNGLTNSVLNQLTGEYSPVPVTAKAYR
                     DTGTGSVVIAEENFGEGSSREHAAMEARFLGVKAIIAKSFARIHETNLKKQGVLALTF
                     MNPEDYDRVREDDRVDITGITKMAPGTTLKLILRHSDGTKEE"
     assembly_gap    4556..4655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4890..6155
                     /gene="icd"
                     /locus_tag="PLG42_04990"
     CDS             4890..6155
                     /gene="icd"
                     /locus_tag="PLG42_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOU30601.1"
                     /translation="MNPGKITFENGQLLVPDNPVIPFIEGDGTGPDIWRAAVRVIDAA
                     VCKSYRGKRKIEWMEVLAGEKAFNLTGKWLPEETVTAFREYFVGIKGPLTTPVGEGIR
                     SLNVALRQELDLYVCYRPVRYYRGVPGPVKHPELVDMHIFRENTEDIYAGIEFMVGEP
                     ATEKFRDFLISEMGVKKIRFPRTSSFAVKPVSVEGTERLVRQAIRFAIERKLPSVTLV
                     HKGNIMKFTEGAFMKWGYALAEREFGEFVFTWEQYRAIEKAGGTAKADEELKKAGEEG
                     KVIIKDVITDAFLQQILTRPAEYSVIATLNLNGDYISDALAAIVGGIGIAPGANINFD
                     TGHAVFEATHGTAPKYAGQDKVNPGSVILSGALMLEYIGWNEAARKIHDGLEAAISAG
                     KVTYDFHRLMENAVLLKTSEFADEIIKAM"
     gene            6168..7526
                     /locus_tag="PLG42_04995"
     CDS             6168..7526
                     /locus_tag="PLG42_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOU30602.1"
                     /translation="MNGNDFFRMFEESVRESCQIDNELFEKYDVKRGLRNSDRTGVLV
                     GLTNIGDVVGYEKKDGKVIPVPGRLLYRGIDIDEITHGFQKENRHGFDETVFLLLSGR
                     LPTKSELEQFSSYLASLRPLPDHFINNMILSLKGKDVLNILARSVLGLYTLDDDPDNI
                     SLDNMIKQSLNLIAKFPCIIAYSYQALRHAYYGKTLSIRHPLMHLTTAENFLHLIKGD
                     KAYTKLQADMVDLALVLHAEHGGGNNSTFTMRVTSSTETDIYSAVTAAIGSLKGPLHG
                     GANLKVLHMMENIKENVKDWKDKEEVENYLMKILRKEANDFSGKIYGVGHAVYTISDP
                     RAVLLKEKARELAAEAGRLDEFDLYCLVEELTPDVFRKFKGDQNDKTVCVNVDFYSGF
                     VYSCIGIPKDLYTPLFAMARIAGWCAHRIEELTFSAKRIIRPAFKNVYGIRPYVPLNE
                     RI"
     gene            7733..7894
                     /locus_tag="PLG42_05000"
     CDS             7733..7894
                     /locus_tag="PLG42_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30603.1"
                     /translation="MAFFISPTASPGIATGRGLSEEKRHKVPVRGNAGGGRGCPSSTL
                     TWDGEWVKE"
BASE COUNT         2460 a         1580 c         1896 g         2000 t
ORIGIN      
        1 cgtatcaccg taaaggttgg aagcaacgtg ttgaccaatg acgatggttc actcaacaca
       61 tggagaattt ccagaattgt tgaagattta gctatccttt acaggaatgg gatagaggtt
      121 attcttgtaa catcaggtgc tgttgctgct ggaaggtcag aaataacccc atcgaggaag
      181 accagtgtgg tggagtcgag gcaaatactt gctgcagtgg gacaggtaaa actgatgtcg
      241 cattaccagt atttattcgg aaaatatgga attcctgcag gacaggttct cgccacaagg
      301 gaaagcttta tggacaggcg gcactatctg aacatgaaaa actgcatcat ggcaatgctc
      361 gataacaaaa taatccccat tgttaatgaa aatgatacta tctcagtaaa cgagctgatg
      421 tttacagata acgacgaact ttcctgcctt atcacttcaa tgatggattg cagctcactt
      481 ataatcctca ccaatgttga cggtatctat acaggtatcc ctggtgatga tgggacggag
      541 cttataaggg aggtgcgtcc tgatgcagaa ggaattgaac aatacatctt ttcattccgc
      601 tccgaattcg gacgtggcgg tatgcttaca aaatactcaa atgcccgcag acttgcctcc
      661 gagggtgtca gagtttttat tgcaaacggt ttgcgcgatt ccattataac tgatatcgtg
      721 cgcgggaaag atgtaccata tactcacttt ttgcctgctg gcaaacgcag gaacagtgtg
      781 aagaaatgga tagcccattc cgctaccttc gcccgtggtg tggtggttgt caaccgggga
      841 gcaaaggagg ccctcatggg ccctaaagct tcaagtcttc tgcttgtagg ggtaacccgc
      901 atcgaaggat ctttcaaaaa aggagatatt gtcagaataa ttgacgaaga agggaactct
      961 ataggcctgg gcaaatcgga atacgattcc gataaagcaa ttcagaatat aggtcagaaa
     1021 tgcagcaaac cactggtaca ttaccattat ctggttttaa atgagaatgc aggtgttgaa
     1081 cctgtggttt tttaattata caatccctga gtacttatga actgcatccc tcttttaaaa
     1141 agggccgcag aagccggccg taaaatgact gttattgaca acagcaggat aaatgaggta
     1201 cttctgaaac tggctgatgc cgttgaggaa aatatccctt ttatcatttc cgagaatgag
     1261 aaagacctca ggaaaatgga ccagaatgac cccaggtacg acaggctact tctgacaaaa
     1321 gaaaggatag aaggtatagc atccgatatt agaaatgttg catcactgcc atcaccggtc
     1381 ggacgcatac tcgaccagcg ggtgcgccca aacggactta aaatctcaaa agtgagcgtg
     1441 ccgttcggcg tggtggcagt tatttatgag gcaaggccaa acgttacgct cgatgtcttt
     1501 tcactctgct tcaaatcagg caatgcatgt gttcttaaag ggggaagcga tgcaataaat
     1561 tcaaataagg ctctggtaag catcattgcc ggaatactgg aaaaagaagg aattgacaaa
     1621 aatgttgtaa cccttctccc ggcaggacgc agtgaaacca tgcaactact gaaagcacgc
     1681 gattatatag atcttatcat cccacgcgga ggaaaatcac tcatcgattt cgtaagggaa
     1741 aacgcgcttg ttcctgtgat agaaacagga gccggcatct gccatacata ttttgacgag
     1801 tatggcgacc gggaaaaggg aagattaata atcaataatg caaagacccg cagggtgagc
     1861 gtctgcaatg cactcgactg ccttatagtc catgaaaaac gactctctga cctgccttat
     1921 ctggtaagtc tttgcgccga aaagaatgta ataatctatg ccgacagaaa agcattttca
     1981 gccctgaaag gaaaataccc tgcacacctg cttcagcatg ctacaaaaga atcgtgggga
     2041 acagaattcc tttcttataa gatggcggtt aaaaccactt cttcatttga tgaggcaatt
     2101 agccatatat cgaggtatag ctcaaagcac agcgaagcta ttatttcgga agatccagac
     2161 agaataaaga tgtttttcaa tcttgtcgat gcagctgctg tatattccaa tacgtcaaca
     2221 gctttcaccg acggggcaca gttcggcctt ggagctgaaa tagggatttc aactcaaaaa
     2281 cttcatgcaa gaggtcctat ggcactcgaa gagctaacaa cctataaatg gattattgaa
     2341 ggaaacggtc agataagaag ctgaaacatt caataactgg catgtacgat tttgaaatta
     2401 taaaagactt ctatgaagcg ctgcctgaga gaatgagaaa aatcaggaaa agagcaggcc
     2461 ggccgcttac tcttactgag aaaattctgt tcaatcattt gtatgagaat ggggcagccg
     2521 gtgatttaaa tagaagcgga cagtatgctg atttcaaacc cgatcgcctt gcaatgcagg
     2581 atgccactgc tcagatggca ctgcttcagt ttattcttgc aggaagcaaa aggtctgcca
     2641 ttccggcttc agttcactgc gaccacctga ttgtggctga aaagggaggg gaagaggatc
     2701 ttaaaaaagc tgttgaaact ggcaaagaag tttatgaatt cctcgaatca gtctgcagaa
     2761 agtacggaat aagcttctgg aagcccggct caggaataat tcaccagata gttcttgaaa
     2821 actatgcctt cccgggaggt atgattacag gtacagattc gcatactcca aatgctggag
     2881 gccttggtat gataggtgtg ggagtaggag gagccgacgc agtggatgcc atgacaggca
     2941 tgccatggga attgaagatg ccagaaataa tcggcgtgag actcacagga aggctctcag
     3001 gatggtgctc accaaaagac gttattctga agctggccgg tatacttacg gtaaaaggtg
     3061 ctacaggttc ggttatcgaa tatttcggcg atggaacttc aacaatctcc tgcaccggaa
     3121 aagccacaat ttgcaacatg ggggcagaga ctggtgcaac atcatctgtt tttcctttcg
     3181 atgaatcaat ggttcattat ctgaattcca ccggaagaaa tcaggttgca gaactcgcat
     3241 cgaaaaacag gatgcatctg caggctgacc ctgaagtcta tgctgctcct gaaaaatatt
     3301 acgacagagt gattgagatc aatctctcgg aactcgaacc tcatctcaat ggccctttta
     3361 cacccgacct tgccactact gtgcaggaga tgaagaaaaa agcagagaag cataattggc
     3421 cactggatgt tgaggcagta ctgattggtt catgcaccaa ctcttctttc gaagacctgt
     3481 ctaaagctgc ctcagtggta aaaaatgctc ttgaaaagaa actgaaacca atagcaaaga
     3541 tttatgtatc accgggttca gaatttgtcc gcactcttgc agcaagggag ggtatccttg
     3601 atctgttccg cagggcagga gcagaaatat ttgcaaatgc ctgcggacca tgcatcgggc
     3661 aatggaaaag gcaaacagat gaaaatgaaa gagtcaatac aatagtgcat accttcaatc
     3721 gtaatttttc aaagcgtact gacggtaacc ctaataccca tgtgtttgtt gcttcgccgg
     3781 aaattgccgc tgctattgca ttttcaggaa aactttcatt cgacccttcg agagataaaa
     3841 tcgtgaatga agaaggtaaa ttagtaatgc ttgacgagcc cctggctaaa gaacttccgg
     3901 aaaatgctgc aggtttaatg tctgaaacag ttgcagtaaa aaatattcag gatgaagata
     3961 cagagataat tataccgcct aactctgaac ggctgcaatt actgaagcct ttcgaaccct
     4021 ggaacggaaa ggattttcaa ggcttatatc ttcttattaa agtaaaaggt aaatgtacaa
     4081 ccgaccatat ttccatggca gggccatggc ttaaataccg tggacatctg gaaaatatct
     4141 ctgagaatta catgatcggt gccgtaaacg cattcaatgg tttaacaaac agtgttttaa
     4201 accagttgac cggagaatat tcaccggttc ctgttacagc aaaggcttac agagataccg
     4261 gcaccggttc tgtagttata gcagaagaga atttcggaga gggttcatcg cgtgagcatg
     4321 ccgctatgga ggcaagattt cttggtgtaa aggcaattat tgcgaagtcg tttgcccgca
     4381 ttcatgagac taatcttaag aaacaggggg ttcttgctct gacttttatg aatccagaag
     4441 actacgaccg ggtgagagag gatgaccgtg ttgatataac cggaataacg aagatggcac
     4501 ccggaaccac tctgaagctt atactaaggc attcagatgg aacaaaagag gagatnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccgga accactctga agcttatact
     4681 aaggcattca gatggaacaa aagaggagat aactcttttg cactcatata gcgaaaaaca
     4741 gattgaatgg ttcagggaag gaagtgcatt gaatgtgata aggaaactga atacttaaat
     4801 aagtggataa ttcagaaata caaaggaaat tagtgaagaa acttaataaa gtacttcaaa
     4861 ttgcgagtaa aaaaacagaa tgatcaatta tgaatccggg aaaaataaca tttgaaaatg
     4921 ggcagctcct tgtgcccgat aatcctgtaa ttccatttat tgagggtgac gggacgggac
     4981 cggatatctg gcgtgcggca gtgcgtgtaa ttgacgctgc agtgtgtaaa tcttacaggg
     5041 gaaaaaggaa gattgaatgg atggaggtgc tggcaggcga gaaggcattt aaccttactg
     5101 gaaaatggct gcctgaagag acagtgacag cattcaggga atactttgtt ggtatcaagg
     5161 gtcccctcac caccccggtg ggcgagggaa tacgctcgtt gaatgtggca ttgaggcagg
     5221 aactcgacct ttatgtatgt taccgccctg taagatatta cagaggagtc ccaggcccgg
     5281 taaaacaccc cgagcttgtc gatatgcata ttttcaggga aaatactgaa gatatttatg
     5341 ctggcatcga gtttatggta ggtgagccag ccactgaaaa attcagggat tttctcattt
     5401 cagaaatggg agtaaaaaaa ataagatttc cccggacatc atctttcgcc gttaaacccg
     5461 tatcagttga aggaaccgaa cggcttgtcc gccaggcaat aaggtttgct attgagagaa
     5521 agcttccttc ggtcacgctt gttcacaaag ggaatattat gaagtttact gaaggggctt
     5581 tcatgaagtg gggatatgca cttgccgaaa gggaattcgg agagttcgtt tttacatggg
     5641 aacagtaccg tgcaattgaa aaagcaggtg gtacagcaaa agctgatgaa gaacttaaaa
     5701 aagccggaga ggaagggaag gtgataataa aggatgtcat caccgatgca tttcttcagc
     5761 agatacttac gcgccctgct gaatattctg ttatagctac cctgaatcta aatggcgatt
     5821 atatctccga tgcccttgct gcaattgttg gggggatagg tatagccccg ggggcaaaca
     5881 taaacttcga caccggtcac gctgtgtttg aagctactca tggcactgca cctaaatatg
     5941 ccggacagga taaagtaaat cccggttcgg taatcctttc aggtgcatta atgcttgaat
     6001 atataggatg gaatgaagct gcacgaaaga ttcacgacgg ccttgaggcc gcaatctcgg
     6061 caggtaaagt aacatacgat ttccatcgtc ttatggaaaa tgccgtgttg ctcaagacat
     6121 ctgagtttgc tgatgaaata ataaaggcta tgtaaaattt taagaagatg aatgggaatg
     6181 attttttcag aatgtttgag gagtctgtac gggaatcgtg tcagatcgat aacgaacttt
     6241 tcgagaagta cgatgttaag cggggattga gaaacagtga ccgtacaggt gttcttgtgg
     6301 gacttactaa cataggcgac gttgtcggct atgaaaagaa ggacggtaag gttatccctg
     6361 tcccgggcag gttgctttac aggggtatcg acattgatga gataacccat ggttttcaga
     6421 aagaaaacag gcacggtttt gatgaaaccg tttttctgct gctgtcagga aggcttccga
     6481 caaaaagcga actggagcaa ttctcttcat atctggcttc actcaggcct ctgcccgacc
     6541 atttcataaa taacatgatt ctctccctta agggtaaaga tgtgctcaac atacttgcaa
     6601 gatctgtgct cggactctac actcttgatg acgacccgga taatatttca cttgataaca
     6661 tgataaagca atcactcaat ctgattgcca aatttccgtg tataattgcc tattcatacc
     6721 aggcactcag gcacgcatat tatggaaaga ccctgtcgat tcgccatcct ctgatgcacc
     6781 tgactacagc agaaaatttc ctgcatctca tcaagggtga taaagcttac acaaagcttc
     6841 aggccgatat ggtcgacctt gcactggtat tgcatgccga acatgggggc ggtaataact
     6901 ccacatttac gatgagggta acaagttcaa ccgaaactga catttattca gcagttacgg
     6961 cagccatagg ctccctgaag ggcccgcttc acggcggagc aaacctgaaa gtgctgcaca
     7021 tgatggaaaa tatcaaggaa aacgtaaaag actggaaaga caaagaagaa gttgaaaatt
     7081 acctgatgaa aatattaagg aaagaggcaa atgacttttc aggtaagatc tacggtgtgg
     7141 gacatgcagt gtatacaatt tccgacccgc gggctgtact tttaaaagaa aaggcacgcg
     7201 aactggctgc agaggctgga cgactcgatg aatttgatct ttactgcctt gtagaggaac
     7261 tgacacctga tgtcttcaga aaatttaaag gcgaccagaa cgataaaacc gtatgtgtga
     7321 atgtcgactt ctactcgggt tttgtatatt cctgcatagg gattccgaaa gacctctata
     7381 caccgctctt tgctatggca agaattgcag ggtggtgcgc tcaccgtatt gaagaactta
     7441 ccttctctgc aaaaagaatc atcaggcctg catttaaaaa tgtttacgga atcagacctt
     7501 atgtgccatt aaatgaaaga atataaaaaa tattttaaaa aattttcatt ttttctttgt
     7561 atgtatttaa aaaaatatat ttttgtaacg gaataacaga caatgataaa cgttttatat
     7621 acaggcacag gtaccaccac cacaacagga acactgttgt accggggatg ccttgctctg
     7681 taataaggat tgttaaataa tgtaattgca gccatccccg gaaaaaccgg ggatggcttt
     7741 ttttatatct cctactgctt ctcccggcat tgcaactggg agaggcctga gtgaagaaaa
     7801 aaggcataaa gtcccagtca ggggaaatgc aggtggcggc agaggatgcc cctccagcac
     7861 cctaacgtgg gatggtgaat gggtaaaaga ataaaggaga tcgaataatg ttgttgttca
     7921 gatatttata aaaatcattt caatgaaaat cctgaaattc ggaggcactt ctgttgccga
     7981 tgctgacaag attagaaaag ttgttgaaat catccggctt aatatctcgg aaggag
//
LOCUS       DAOSNB010000087         8033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_8960_length_8033_cov_4.761594, whole genome shotgun sequence.
ACCESSION   DAOSNB010000087 DAOSNB010000000
VERSION     DAOSNB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8960_length_8033_cov_4.761594"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1835)
                     /gene="ggt"
                     /locus_tag="PLG42_05005"
     CDS             complement(114..1835)
                     /gene="ggt"
                     /locus_tag="PLG42_05005"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU30604.1"
                     /translation="MSFCKGIICISVLFVLLISGCSRNEADNSNSLFSEGQFITSDSG
                     MVVSAHPESSKVGINILRKGGNAMDAAAAVEFALAVCYPEAGNLGGGGFMVIRKASGE
                     TDAIDYREKAPAASRRDMFLDSAGNVIRGLSTNSHLACGVPGTVDGIIKVHSKYGKLP
                     FNEIIQPAIDLAEKGFPVTAVQAKSLNANRINFIERNRIRPAFVKDSLWKEGDTLIQP
                     ELANTLKLIRDYGRDGFYKGITAQRIEAEMKRGNGLITSEDLASYDAQWREPVTGTYR
                     GYRIIAAPPPSGGGIILLQLLGMVENFPLRKWGFHSAKAIHIMIEAEKRAFADRAEFL
                     GDPDYVKIPVNGLINKRYLKGRISNVDMRKASSPEEITAGNPEWIESEETTHYSVVDA
                     FGNAVAATTTLNATFGTSIVVEGAGFLLNNQMDDFSIKPGYPNIYGLTGSEANSIQPG
                     KRMLSSMTPAIVEKNKKLFLILGSPGGSTIPTSVFQVIVNVIDFRMSIKDAVIAGRFH
                     NQWLPDQTDMEKESIDSLTIAVLRNLGHKFYTRSAIGRVNAIMIDKNGLRHSGADPRG
                     NNVACGY"
     gene            1972..3276
                     /locus_tag="PLG42_05010"
     CDS             1972..3276
                     /locus_tag="PLG42_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOU30605.1"
                     /translation="MLANQPLADRMRPQDLDEFCGQEHLVGENSVLRKAIETGNIPSF
                     IIWGPPGVGKTTLAWIIANRLKRPFYQLSAVHSGVKEVRETIEKAKKQQFFNRPNPIL
                     FIDEIHRFNKSQQDSLLNAVEKGILTLIGATTENPSFEVISPLLSRCQVYIMNPLTDE
                     DLIKLINKAIIKDPYLSKYKIHIEETEALLRAAAGDARKLYNALELVVTSELDEHPDG
                     DIRITNEKVKAVIQKNLALYDKGGDQHYDIISAFIKSIRGSDPNAAVYWLARMVEGGE
                     DPLFIARRMVILAAEDIGLANPNALLMAQTCFEAVNLIGWPEARIILSEAAIYLAVSP
                     KSNSSYMAIEKAQSKVRETGDLSVPLHIRNAPTRLMKELGYGKEYKYAHDYDGNFIRD
                     NFLPEEIKGTIFYEPGENPREAEIRKKIASMWKDFYNYKPEI"
     gene            3327..4139
                     /gene="kdsA"
                     /locus_tag="PLG42_05015"
     CDS             3327..4139
                     /gene="kdsA"
                     /locus_tag="PLG42_05015"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOU30606.1"
                     /translation="MNIPNIKYLDSGNFLLIAGPCVVENREVTFTTASFLTELAEKYS
                     VPFIFKSSYRKANRSKSDSFTGIGDIPALEILAEVRKEFSVPVITDIHNPHEASIAAK
                     YVDVLQIPAFLCRQTDLLVAAAMTGKWINIKKGQFVSGRSMRFAVEKIRNAGNNNIML
                     TERGNSFGYNDLVVDFRNIPEMKSIGVPVIVDITHSVQQPNQESGVSGGRPEMIETLG
                     RAAIAAGADGVFMETHPDPASALSDGANMLKLSEMEKLLAKLVAIRRTVISF"
     gene            4171..4851
                     /locus_tag="PLG42_05020"
     CDS             4171..4851
                     /locus_tag="PLG42_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30607.1"
                     /translation="MKKSVLIILIFLAALAAVQAQSLEEIVKRQSQALKEEAFDNIKT
                     LKITGNLSQSGMIIGMTTYYKFPEKSRIALSFNGQEVIQLYDGNKAYLINPMAGGAPQ
                     ELSPDQAANLKNNSTFRSPLARYLKEGKLTLEGTENVNDKPAFKIKAVDGGTIMYFFI
                     DKSSWLPVKTTVIQNGINVDTFQEWGDLDGIILPKVSTTKAGNIEITIKIEKAEINIP
                     LEDSLFKI"
     gene            complement(4901..5278)
                     /gene="rnpB"
                     /locus_tag="PLG42_05025"
     ncRNA           complement(4901..5278)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLG42_05025"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5483..6736
                     /locus_tag="PLG42_05030"
     CDS             5483..6736
                     /locus_tag="PLG42_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOU30608.1"
                     /translation="MTGERKRRGLPDIDIENFKYDLPQNRIAQYPLPERDKSKLLIYK
                     NGFISETVFSDIDKEIPSGSVMIFNNSRVIKARLFFEKQTGSIIEIFCLEPVMPADFS
                     AAMESKGETEWKCIIGNLKKWKQGKIKCQYKTAGDSYIVWAEKLYPGEDNTWIVRFTW
                     DSGNFTFKEFIDSAGHVPLPPYIIRPDEPSDRKWYQTIYSSVDGSVAAPTAGLHFTDR
                     VLEKLNSKGIRQLNVTLHVSAGTFRPVKSKSIQGHEMHSELFFVEKEIIIHLLSNKGP
                     IVSVGTTSLRVLESLYWAGIKLKMKPDTDPDSLYIDQWDPYLLKCKLKPEESLEVLLE
                     WMEKNKTGRLSALTKLFIFPGYKFMFSDILLTNFHLPKSTLLMLVAAFTGSDWKKIYE
                     YALGNNYRFLSYGDASLLFRKDVIF"
     gene            7151..>8033
                     /locus_tag="PLG42_05035"
     CDS             7151..>8033
                     /locus_tag="PLG42_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOU30609.1"
                     /translation="MKIVSYIQNFIIIACHLIKPHYKAFMNHNSEIIFRIFIVPVLLI
                     SLHSCIGGSNKSYPTDTPTSGHLKILVDDSFKPLLEAEIAVFTELYRNATIIPSYKPE
                     VDVINEFLRDSGKVMVTSRKLTDDQIKYLRDSLIIARTTTFAYDGIAIILNKANPDSL
                     LEYEDVKNIFTGKIKSWKEINNKSPLGNIYVIFDNAKSGNIRYFREKFDYYDPLPSNF
                     YAVNSNPEVIDYVEKNKNALGIVSVNWISDPDDSLSMSFINKIKVAAISHPFFEKGTY
                     YRPYQGSIYDKSYPFTRE"
BASE COUNT         2608 a         1483 c         1492 g         2450 t
ORIGIN      
        1 taggtcaaac aaccttaaaa tatcaaaatt taattgttaa aagtattaat ttattttcag
       61 taaagaaaaa aattttctac caatttagta aaaaataatt aacatattac caatcaatat
      121 ccacaagcaa cattatttcc tctcgggtca gctcccgaat gacgtaaccc atttttatca
      181 atcataatcg catttactct accaatggca gaacgggtat aaaatttatg ccctaaatta
      241 cgtaaaacag ctattgttaa tgaatcaatg ctttcttttt ccatatcagt ctggtcggga
      301 agccattggt tatggaaacg tcctgcgata acagcatctt ttatgctcat cctgaaatca
      361 atcacattca cgataacctg aaacactgat gttggaattg ttgaacctcc aggtgagccg
      421 agaatcagaa aaagcttttt gtttttttca actattgccg gtgtcatcga actcagcatt
      481 ctcttgccag gttgtattga attggcctca ctgccggtaa gtccataaat atttggataa
      541 cctggtttta ttgaaaaatc gtccatctga ttattaagca gaaatccagc tccttcaact
      601 actatagatg ttccgaaggt tgcgttaagg gttgtggttg ccgctacggc attcccaaat
      661 gcatcaacaa ctgaataatg agtagtttcc tctgattcta tccattcagg atttccggct
      721 gtgatttcct ctggcgatga agcttttctc atgtcgacat tgcttattct tcctttcaga
      781 tatcttttat taatcagtcc atttaccggt attttaacat aatccggatc tcccaggaat
      841 tcagctctat cggcaaatgc tcttttttcg gcttcaatca ttatatggat tgctttagca
      901 gaatgaaaac cccattttct taaagggaaa ttttcaacca tgccaagtaa ttgaaggagt
      961 attattcccc ctcctgaagg tgggggtgcc gcgataattc tgtatccacg atatgtaccg
     1021 gtaacaggtt ctctccattg tgcatcgtat gaggcaaggt cctcagacgt aattaaccca
     1081 ttacctcttt tcatttcagc ctcaattcgt tgtgctgtta ttcctttata aaaaccgtca
     1141 cgaccataat cacgtataag tttaagagta tttgccagtt ccggttgtat caatgtgtct
     1201 ccttctttcc acaatgaatc tttcacaaag gcaggcctga ttcggtttcg ttcaataaaa
     1261 tttattctgt tggcattaag cgattttgcc tgaactgcag taacaggaaa tcccttctca
     1321 gcgagatcaa tagctggctg aattatctca ttaaaaggaa gctttccata ttttgaatgt
     1381 acttttatta tcccgtcgac agtacccggt actccgcaag caaggtggga atttgtactc
     1441 aatcctctta taacgttacc ggcagaatca agaaacatat cacgccttga cgcagccggg
     1501 gctttttctc tgtaatctat agcatcggtt tcaccgctgg cttttctaat taccatgaac
     1561 ccacctcccc ctaaatttcc tgcttcagga taacatacag ccagggcaaa ttcaacagca
     1621 gcagctgcat ccatagcatt cccgcctttc ctgaggatgt ttatacctac ttttgagctc
     1681 tctggatgag cagaaacaac catcccacta tctgaagtaa tgaactgccc ttcactaaac
     1741 agactatttg aattatcagc ctcattcctg ctgcatccag aaattagtaa tacaaaaaga
     1801 actgaaatac agataattcc tttacaaaag ctcatgatga ttctacagta tttaataaat
     1861 gtctgtaaat atgtaaaaaa agttgctttg tatctcttaa atttgtgatt atattaattc
     1921 ctgctgaaat aaaaagtgtt tttaatactt ttacatggat aataagtaaa gatgttagcc
     1981 aatcaaccat tagccgacag gatgaggcct caggaccttg atgagttttg tggccaggaa
     2041 caccttgtgg gtgagaactc agtattgcgg aaagctattg aaacaggaaa tataccttct
     2101 tttattatat ggggtcctcc cggtgttggt aaaactactc ttgcatggat tattgcaaac
     2161 aggttaaaac gtcctttcta ccagctaagt gcagtacatt caggagtgaa ggaagttaga
     2221 gaaacaatag aaaaagcaaa aaaacaacaa tttttcaaca gaccgaatcc tatcttgttc
     2281 attgatgaaa tccaccgatt caataaatca cagcaggatt cacttctgaa tgctgttgaa
     2341 aaagggatac ttacactgat aggagcaaca acggaaaatc catcttttga agttatttcc
     2401 cctctcctct cccgttgcca ggtatatatt atgaatccac ttacagatga agaccttata
     2461 aagctcataa acaaagcgat aataaaagat ccatacctgt caaaatacaa aatacacatt
     2521 gaagaaacag aagcactttt aagagcagcc gcaggtgacg caagaaaact gtataatgca
     2581 cttgagctag ttgtaacatc tgagcttgat gagcaccctg atggcgatat aagaattaca
     2641 aacgaaaaag tgaaagcagt tatccagaaa aaccttgctt tatatgataa aggcggtgat
     2701 cagcattatg acattatatc agcattcata aaatctatca ggggaagcga ccctaatgct
     2761 gctgtatact ggcttgcaag aatggtagaa ggaggtgaag atccgttatt tattgccaga
     2821 aggatggtaa ttcttgctgc agaagatata ggactggcaa atcccaacgc tctgttaatg
     2881 gcccaaacat gttttgaagc tgtcaattta ataggttggc ccgaagccag aataattctt
     2941 tctgaagcag ccatttatct tgctgtgtcg cctaaaagta attcatccta tatggctatt
     3001 gagaaagccc agagtaaagt aagagaaaca ggcgaccttt ctgtaccact tcatataaga
     3061 aatgctccaa caagacttat gaaagaactt ggttacggta aggaatacaa atacgctcac
     3121 gattatgatg gtaattttat aagggataat tttttacccg aagaaattaa aggtacaata
     3181 ttttacgaac caggtgagaa tcccagagaa gcagaaataa gaaaaaagat tgcttcaatg
     3241 tggaaagact tttacaatta caaaccggaa atttaactgg taagtttttt tgattacctt
     3301 tacctgctta aaaaagaaat ttacaattga atatacctaa cattaaatat ctggattccg
     3361 gaaattttct tctgatagcc ggaccctgtg tggttgaaaa cagggaagtt acatttacta
     3421 ctgcatcttt tcttacagag cttgccgaaa agtattctgt tccgtttatt tttaaatctt
     3481 cataccgaaa agctaacagg tcaaaatctg actcattcac agggattggt gatattcctg
     3541 ctttagaaat tctcgcagaa gtgcgtaaag agttttctgt tcctgtaata actgatattc
     3601 acaatcctca tgaagccagt attgcagcta aatatgttga tgttctgcag attccggctt
     3661 ttttatgcag gcaaacagat cttctggtag ctgctgcaat gacgggtaaa tggataaata
     3721 tcaaaaaagg tcagtttgtt tcaggcagat caatgcgttt tgcagtggaa aaaattcgta
     3781 atgcaggtaa taataatata atgctgactg aaagaggcaa ttcatttggt tataatgatc
     3841 tcgttgtgga tttccgtaac attcctgaaa tgaaaagtat aggtgttccg gtgattgttg
     3901 atataacaca ttcggttcag cagcccaacc aggagtcggg tgtaagcgga ggcaggccag
     3961 aaatgataga aacactcgga agagctgcta tagccgctgg agccgatggc gtattcatgg
     4021 aaactcatcc cgacccagca agtgctctgt cagacggtgc caacatgctt aaactatcag
     4081 aaatggaaaa attgttagca aaacttgtcg ctataagaag aaccgttata tcattctaaa
     4141 ttaattattt acaaattaaa aatgaaattt atgaaaaaat ctgttcttat tattttaatc
     4201 tttctggctg cacttgcagc agttcaggca caatccctgg aagaaattgt aaagaggcag
     4261 tcgcaggcat tgaaagaaga ggcttttgat aatatcaaga cattgaaaat tacaggaaat
     4321 cttagccagt caggtatgat tatcggaatg acaacttatt ataaatttcc tgaaaaatcg
     4381 agaatagccc tttcctttaa tggacaggaa gtaattcagt tatacgatgg gaataaagct
     4441 tatttaataa atccgatggc tggaggagcc cctcaggaac tttcacctga tcaggctgca
     4501 aacctgaaaa acaacagcac tttcagaagt ccgctggcac gctatctcaa ggaaggaaaa
     4561 ttaacactcg aaggcaccga aaatgttaat gataaacctg ctttcaagat aaaggccgtt
     4621 gatggtggca ctattatgta tttctttatt gataaaagct catggctacc ggttaaaacg
     4681 acagtaattc agaatggaat aaatgtcgat acttttcaag aatggggtga tcttgacggt
     4741 atcattctac caaaagtttc aaccacaaaa gctggaaata ttgaaatcac aattaaaata
     4801 gaaaaagcgg aaatcaatat tccgcttgaa gatagtttgt ttaaaatctg aatttatttc
     4861 cgggtaaaca accaaaaggg agaattctcc cttttgttgt ttatgcagct ggcctgtaag
     4921 ccgggttctg tagggattct ttcttctact cgagagcccg gaatcccttt ccatcatcta
     4981 tctggtcccg tcattgctgg cgggatcata cgacctaccc cccgacatcg gacgagcagt
     5041 cctcaggcgc cggtatacat ggtctttcaa cccataaggt gcacggcttc ctgtgtcacc
     5101 acaggaaccg gtgagctctt acctcacctt ttcaccctta tcccgacagg gtcgggacgg
     5161 ttattttctg ttgcacttct ataccctcac gggtatcttc cggttaggaa gtatggtgct
     5221 ctgtgttgcc cggactttcc tttccgcctg tcggcggaac gatggaacgg cctgctgcag
     5281 ttgcaaaatt aataaaaata tgcctgtttt acaacattat tcttgaagag agtaaagttt
     5341 tattattaaa taaataattc attatcaacc tgttatgttc attccaaatc caatattatt
     5401 taataagact ttcttcttgt taataataac aaaattgcta ccgataaaaa tttataactt
     5461 tacgccactt ttaaaaaaag cagtgacagg ggaacgaaaa cggagaggtt taccggatat
     5521 cgatattgaa aactttaagt acgatcttcc acagaaccgt attgcacaat atcctttgcc
     5581 cgaaagggac aaatcaaagt tacttattta taaaaacggc tttatttcgg aaacagtttt
     5641 ttcagatatt gataaagaga taccctcagg tagcgtgatg atatttaata actcacgagt
     5701 tataaaagca aggctttttt tcgagaaaca aacaggttca attattgaaa ttttctgcct
     5761 tgagcctgtt atgcctgccg atttttccgc tgcaatggaa tcgaaaggag aaacagagtg
     5821 gaaatgtata atagggaatc ttaaaaaatg gaaacaaggt aaaataaaat gtcaatataa
     5881 aactgccgga gatagttata ttgtttgggc cgaaaaatta tatcctggag aggataacac
     5941 atggatagta agatttacat gggattccgg gaactttaca ttcaaagaat ttattgattc
     6001 ggcaggtcac gtacctcttc ctccttatat aataaggcct gacgaaccat ctgacaggaa
     6061 atggtatcag acaatatatt catctgtgga tggctcggta gcagctccta ctgctggatt
     6121 gcatttcacg gatagagttc tggaaaagct gaattcaaaa ggcatacgtc aacttaatgt
     6181 aacacttcat gtgagtgcag gtacattcag accggtaaaa tcaaaaagca ttcagggtca
     6241 tgagatgcac tcggaattgt tttttgttga aaaagagatt attatccatc tgttaagtaa
     6301 taaaggtcca attgtgtctg tgggaactac ttctcttagg gttctggaaa gtctttactg
     6361 ggcaggtata aagttaaaga tgaagcctga tacagatcct gactcacttt atatagacca
     6421 gtgggaccct tatttattga aatgtaaatt aaaaccagaa gaatcactgg aagttttact
     6481 ggaatggatg gaaaaaaaca aaaccggaag attatcagca ttaacaaaac tttttatttt
     6541 tcctggttat aaatttatgt tttctgatat tctactgacc aactttcatt tacccaaaag
     6601 cacactgctg atgctggtgg ctgcatttac agggtctgac tggaagaaaa tttatgaata
     6661 tgcattggga aacaattaca gatttcttag ttacggagac gcctcactgt tattcagaaa
     6721 agatgtaatc ttttgataat taattagtaa ttttatttgt ttttaatatg atttttattt
     6781 acttgctcgc caaaattttc aggaatgaaa caaaggaaaa taatggatca gattagtgcc
     6841 atttttggca tatttatgac ttttttttac attattgtgg gaattttttt catatatgct
     6901 aagagtttat attatataga cagtttgatc aggaatatcg tcggaatatc ttttcttatt
     6961 ttcagtttgt atagaggata tgtctcctat ttgaaaataa aggaagcttt ttttggaaaa
     7021 ggcagaagaa ataatgagag agaagattac ttttaagtaa ttctgttgaa gattaattca
     7081 tttaattgga tatggcatgc tctttgctta ttatgaaaac agagttactt aaaatttaaa
     7141 acagaaagcc atgaagatag tttcatatat tcaaaatttt attattattg catgccattt
     7201 aataaaacca cattataaag cttttatgaa tcataattct gaaataatat tcagaatctt
     7261 tatagtacca gttttactta tttccttgca ttcatgtatt ggaggaagca ataaatcata
     7321 tcccactgat acacccacca gtgggcatct gaaaatactt gttgatgatt cttttaaacc
     7381 tctgcttgaa gcagaaatag cagtatttac agaattgtac agaaatgcaa caattattcc
     7441 atcatataaa ccagaagtag atgtaattaa tgaattcctg agagattccg gaaaggtaat
     7501 ggtaaccagc agaaaactca cagatgatca gatcaaatat ctacgtgatt ctcttataat
     7561 tgcacgaaca accacttttg catatgatgg aatagccata atacttaaca aagcgaatcc
     7621 tgactccctt ctggagtatg aagatgtgaa gaatatcttt acgggcaaaa tcaaaagctg
     7681 gaaagaaata aacaataaat ccccgctggg gaatatctac gtcatttttg ataatgcaaa
     7741 atcgggaaac atcaggtact tcagggaaaa gtttgattac tatgatcctt tgccttcaaa
     7801 tttttacgca gtaaactcaa atccggaggt aattgattat gttgaaaaaa acaaaaatgc
     7861 tcttggtatt gtaagtgtaa actggataag tgaccctgat gattcattaa gtatgagttt
     7921 tatcaacaaa ataaaagtag cggctatttc ccatccattt tttgaaaaag gcacttatta
     7981 tcgtccttat caggggtcga tctatgataa atcctatccc tttaccaggg aaa
//
LOCUS       DAOSNB010000088         7996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9013_length_7996_cov_5.370105, whole genome shotgun sequence.
ACCESSION   DAOSNB010000088 DAOSNB010000000
VERSION     DAOSNB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_7996_cov_5.370105"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLG42_05040"
     CDS             complement(<1..231)
                     /locus_tag="PLG42_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOU30610.1"
                     /translation="MAEDLRDVFRTGDPHYTPEQGWDAWQAFCSKWGEDYLVIKRLGN
                     DPFYRYYFTYLNYHPRVQSMIYTTNWIERLQRD"
     gene            complement(386..1135)
                     /gene="istB"
                     /locus_tag="PLG42_05045"
     CDS             complement(386..1135)
                     /gene="istB"
                     /locus_tag="PLG42_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOU30611.1"
                     /translation="MDTKTQTIKQYAAQLRLGGIQDQLDQLIQKASEEQCSYLDFSLY
                     LLQTEITQRSLRDLERRTRAAKLPPNHDLDQYDYAFQSGIGKQQLMQLRECQWLEQNY
                     NIVLMGPSGIGKTMIAAGLCFDALKMGYKAYFRTMEQLNEILTMKDITRSALIEYNKL
                     LTANLLVIDDIMMFALEKKQAVQFFNFINHLHERASFIVTTNKSPQEWVKMLDDEVIA
                     TALLDRILFHCEVVRLSGNSYRLENRKTIFN"
     gene            complement(1140..2693)
                     /gene="istA"
                     /locus_tag="PLG42_05050"
     CDS             complement(1140..2693)
                     /gene="istA"
                     /locus_tag="PLG42_05050"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOU30612.1"
                     /translation="MRKKHLNQWIMYHEVHKQHRDGLKPAQIARELVIDRRTVKKYLK
                     MSEDEYLEFIDNQTHRQRLLSSYEGFVRTRLELCPEASAAQVHDWLKENYDDFTEVDA
                     KTVFNFVLHVRSKYGIPKSFDHRDFEKVPELPYGKQTQVDLGEYHMTTDEGKRKKVYF
                     ICFVLSRSRFKYSYYSERPFTTRQVILAHEEAIEYFGGLTEVFVYDQDTLLLIDENKG
                     DLVMTEAFRSYTEYRGFKVHFCRKSDPQSKGKVENVVKYHKFNFLRGRTFIDIFILQA
                     ENLAWLQRTANAKVHSTTKNVPYEEWLTEKAYLKPVRGLYEAKSVTDQRDVSKDNVIS
                     HKGNFYRVPRGTYHPPKTTVRIEENEDNHLIIYNTDNAIIATHTIYPGKGKTIGGTHY
                     QRDLTESIDRLISEITDRFTDPKSARQYCQQIRKEKPRYIRDQMLIIRKLVNNYPVQI
                     VQQAVTFCMENKIYRATDLESVVKRYHSQQSQKDTIEQPIIIKTINQTAHKIIPAKSD
                     ISDYQSLMI"
     gene            complement(2937..3701)
                     /locus_tag="PLG42_05055"
     CDS             complement(2937..3701)
                     /locus_tag="PLG42_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOU30613.1"
                     /translation="MDFTKEQLSEAFVKHLDREKGLQDLMELMIESMMMAERREFLKE
                     SEGNKGNGFRLGRSYGQGRVLEFRIPRDRYGNFHPTILALLRDQEEECELLASSLYCK
                     GLTQSQVGEVFGEVYGKQYSKASISRMIEYLRGDVQQWLSRSLEAYYPIVFVDAIHVK
                     VHRKRSVENEAFYVVMGVKEDKSREVLGIFNRPSESATGWREMFTALEHRGVKGIGLL
                     VADGIRYLEDSLTEVFPGTPLQKCVTHLKRNIVNGK"
     gene            complement(3904..4527)
                     /locus_tag="PLG42_05060"
     CDS             complement(3904..4527)
                     /locus_tag="PLG42_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30614.1"
                     /translation="MALLTQEDGIIESLAAVSYLISASLLFYLFLNSKSEDHPYIFNR
                     KRNPFFLFLSIIFIIFLGEEISWGQRIFNIESPDFFLNGNRQDEINIHNLNVWDAIYP
                     TGEPKRGLIRFFSSVAMYSYFWFAYCLIIPFFSKFSAKAKNLTLRIGFPVIPLLYGIL
                     FIINFITFELLEYSGIGMRPLVEVKETNFALLYLAASFTLLIIYRNK"
     gene            complement(4736..5854)
                     /locus_tag="PLG42_05065"
     CDS             complement(4736..5854)
                     /locus_tag="PLG42_05065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOU30615.1"
                     /translation="MSSDNRNKLFIIVQLPPPVHGASVMNSNVVNSRVIRENFDTIVL
                     NLQFLTSIKEITRFSIRKIAKAFSYSFELAWKIHTVKPDLIYFSFSPKGYSFYRDTLY
                     VFIIKSFRKKLVIHLHGQGVKENQRNNFLRRRLYKAVFRDISVICLSEKLLYDIEDVY
                     DGKPYIVPNGIEPIVTNKLKPIRSSSEISQILCISNYMLEKGIPDLIEAFKIIHDQGF
                     IFSAKLVGAPIDLSIEDLKQQVEKLHLADCIKISGPLYGQEKVNVLLEADIFVLPSRN
                     EAFPLVILEAMQCGLPVVATCEGGIPDMIIENETGLLVEKENPHKLAESIIRLIENKR
                     LRITMGENGLRRFREYYSLEKFETNILSTLNSIIESRV"
     gene            complement(5871..6932)
                     /locus_tag="PLG42_05070"
     CDS             complement(5871..6932)
                     /locus_tag="PLG42_05070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU30616.1"
                     /translation="MSSLTDNGTQQEKVSMLILNDRDLSSGFGGQASFIKNLDPWLRQ
                     KFALTYVTLQSVWLKQKLVPIRLAYLIRVFLFLIRHRHNFDIVLSHTPEASYAVSFFR
                     IPFMHIFHGNLNPVIMSTFWYGKYFRRLFGHFEARIIKKAGHLFTVGEFRPEAEKLFN
                     PVSIDMPGLAGTVRKDFIYAGRLETVKNVDKIIELYSQLPADIKAQNKLNIIGTGSQE
                     KHLQKLVYNLDLNNNVAFHGLTDNDTAIRMIANSRILIIASSSEGFPMVIAESLTCGT
                     PVIATDVGDIRSVLKDGYNGFLLESAYSSVDFSEKVRVILANYNTFSFNSIQSARIFD
                     AKVSANSLISACKKLIRSH"
     gene            complement(6959..7492)
                     /locus_tag="PLG42_05075"
     CDS             complement(6959..7492)
                     /locus_tag="PLG42_05075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU30617.1"
                     /translation="MSVKQKFLQFRLALLRKASNQRKISYFRKQGVRIGENCFINTVA
                     FSTEPYLIEIGNHVAIAGGTEFLTHDGAIWCLREEIENADVFGRIKIGNNVFIGNNCI
                     ILPNTVIGDNSVIGAGSVVRGQFPENSVIIGNPAKTVMSMSMQKLLYTQNPGFLKIKN
                     LSDAEKKKVIKKHFNLE"
     gene            complement(7521..>7996)
                     /locus_tag="PLG42_05080"
     CDS             complement(7521..>7996)
                     /locus_tag="PLG42_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30618.1"
                     /translation="EEPTLELLTKEYDVIIFKHCYPVSLIQADISSPDADSDIKSVEN
                     YQSQYAALKTKMHEFHGTRFILWTPAVNTKHLMTEEEAVRTRQFRDWIVNEWDEKGDN
                     IFVWDFYELETEGGLYLKDKNALSPVNSHPGKLFAGEAALLFGKFITDVIAGEIE"
BASE COUNT         2218 a         1740 c         1569 g         2469 t
ORIGIN      
        1 gtctctctga agacgctcga tccagttagt cgtatagatc atactctgaa ccctcggatg
       61 atagttcaaa tacgtgaagt aatacctgta aaagggatca ttacccagtc tcttgatgac
      121 tagataatct tctccccatt tgctgcagaa cgcctgccaa gcgtcccatc cctgctcggg
      181 ggtgtagtga ggatctcccg tgcggaagac atccctgagg tcttctgcca tcgctccttt
      241 atccccatga cgaaccctgg cgagcatatt tgtaagcggg aacttacaat gtacaaaaac
      301 tggcaaataa ggatgtacga gaaatagcaa ttaaggatgt ccatttcttc atgataaaca
      361 tacaataaaa caaagaacaa cagactcagt taaatatggt tttccggttt tccagtctgt
      421 agctattacc ggatagtctt accacctcac aatggaagag tattctgtca agcaaagcgg
      481 tagcaatgac ttcatcatca agcattttca cccattcctg tggactttta ttagttgtta
      541 cgataaatga agccctttca tgaaggtggt ttataaagtt aaaaaactgc actgcctgtt
      601 tcttttccag ggcaaacatc ataatgtcat caatgacaag cagattggct gtcagaagct
      661 tattgtactc aataagagct gaacgggtaa tgtctttcat agtaaggatc tcattgagct
      721 gttccattgt tctgaagtat gctttatatc ccattttcag agcatcaaag cataggcctg
      781 cagctatcat ggtcttcccg atgcctgacg gacccatcag cactatattg tagttttgtt
      841 ccagccactg acattctcga agctgcataa gttgttgctt tcctatccca ctttgaaagg
      901 cataatcata ttgatcaaga tcatgattag gtggcaattt agcagctctg gtccggcgtt
      961 ccagatcacg gagggatctt tgtgtaattt cagtttgaag gagatataag gaaaaatcca
     1021 gataactgca ttgttcctcc gatgctttct ggatcagttg atctaactgg tcctgtatgc
     1081 cacccagcct gagttgagcg gcatattgct taatggtctg tgtttttgta tccataggct
     1141 taaatcatta gtgattggta atccgatata tcgcttttgg caggtataat tttatgtgct
     1201 gtctgattga tggtcttaat gatgataggc tgctcaatgg tatctttttg ggattgttga
     1261 gagtggtacc gttttacaac actttccaga tcggttgccc ggtagatctt gttctccata
     1321 cagaacgtca cagcctgttg aacgatctgt accggatagt tattgacaag ctttcttatg
     1381 ataagcattt ggtccctgat gtaccggggc ttttcctttc gtatttgctg acagtactgc
     1441 ctggccgatt tgggatctgt aaaccggtcc gttatctcac tgattagcct gtcaatactc
     1501 tcagttaaat ctcgttggta atgggtccct ccgatagttt ttcccttgcc cggatagatt
     1561 gtatgagtag caataatcgc gttgtccgta ttatagataa tcaagtgatt atcctcattt
     1621 tcttcgatac gaactgtcgt ttttggcgga tgataagttc ccctgggaac ccggtaaaaa
     1681 ttccctttat gagatattac gttatccttg cttacatcac gctgatcggt tacacttttt
     1741 gcctcataca agcctctgac aggcttcagg tatgctttct cagtcagcca ttcttcatac
     1801 ggaacatttt tggttgtaga atgaaccttg gcattggccg tacgttgaag ccaggctaga
     1861 ttctctgcct gaagtatgaa gatgtctatg aaggttcggc cacgtaaaaa attgaattta
     1921 tgatacttta caacgttttc aaccttccct ttactctgcg gatcggactt acggcaaaag
     1981 tgtaccttaa atcctctgta ttcggtgtaa ctgcggaacg cttcggtcat aaccagatcc
     2041 cctttgttct catcgataag aagcagggtg tcctgatcat atacaaacac ttctgtaagc
     2101 ccgccaaaat actctatagc ttcttcgtgg gcaaggataa cctgccgggt ggtaaagggt
     2161 cgttctgagt aataactgta tttaaaccgc gaacgggata acacaaagca aatgaaatag
     2221 accttcttgc gcttgccttc atcggtagtc atgtgatatt ctcccagatc aacctgcgtt
     2281 tgcttaccat atggcagctc cggaaccttc tcaaaatccc ggtggtcaaa agacttgggg
     2341 atgccatatt tgcttctgac gtgaaggaca aaattaaaaa ccgtcttggc atcaacttct
     2401 gtgaagtcat catagttctc cttgagccag tcatgaacct gagccgctga tgcctcaggg
     2461 caaagttcca gtctggtacg aacaaatcct tcataggagg atagcaatcg ttgcctgtga
     2521 gtctgattat ctataaattc caaatattca tcctcgctca tcttaaggta cttctttact
     2581 gttcttctgt cgataaccag ttccctggct atctgcgccg gttttaaccc atcgcggtgc
     2641 tgtttgtgaa cttcgtgata cattatccac tgatttagat gtttctttct catagaggat
     2701 cctcctgatt tggattaatg aactgattat taatccaaag aacagaataa tccacttttt
     2761 ccgggaggag tttgcaaact cctcccggaa aacaacacta tcatgaagaa atggaagtga
     2821 tcctgctgag aaaaacacac cttccccaag aaagatgtac atcactattt gctaaaatgc
     2881 gtacatcgct atttgctaat tgtagtacat tcttatttgc gaaaaaacgt acattgttat
     2941 ttgccgttta caatattacg cttaaggtgt gtcacgcact tctggagagg ggttccgggg
     3001 aagacttcgg tcaaagaatc ttcaagatac cttataccat cggcaaccaa cagaccaata
     3061 cccttgacac ctctatgctc taatgccgta aacatctctc tccagccggt tgcgctctca
     3121 ctgggacggt tgaatatgcc aagaacctct ctgctcttgt cttcctttac gcccatgaca
     3181 acgtagaaag cctcattctc aacgctccgc ttgcggtgaa ccttaacatg aatggcatca
     3241 acaaaaacaa tcggataata ggcctccagg ctccgagaga gccattgctg aacatctcct
     3301 ctcagatact caatcatacg actgatactc gccttgctgt actgcttgcc gtatacttct
     3361 ccaaatactt ctcctacctg agactgagta agtcctttgc agtacaggct gcttgccagc
     3421 aactcacact cttcttcttg atctctcagg agtgccagga tcgtaggatg aaagttgccg
     3481 taccggtcgc gaggtatcct gaactcaagt actctaccct ggccgtaact gcgaccaagg
     3541 cgaaaaccat tgcccttgtt cccttcactt tctttgagaa actcacggcg ctcggccatc
     3601 atcatactct caatcatcag ttccatcaaa tcctgcaaac ctttttcacg gtccaaatgc
     3661 ttgacaaatg cttcagaaag ctgttccttt gtaaaatcca ttgggtcttc ttttttggtt
     3721 taacattatt tgcttgtaat gcaaaactaa atcagaagac cttttcttat ggctacgcca
     3781 gacacacttt ttgaaacact atcaaatggg atctgcaatt cactttcttt attagatata
     3841 aaatcatgcc ttggtgaccg gttaaggact gatattttct ttcattaatt ctgaaaggga
     3901 atgttactta tttcggtaaa ttatcaacaa tgtaaaacta gccgctagat aaagcaatgc
     3961 aaaattagtt tcttttactt caactaatgg acgcattcct ataccagagt attcaagcaa
     4021 ttcaaaagtt ataaaattga tgatgaaaag aattccatat aataaaggaa ttacaggaaa
     4081 tcctattcgt aaagttagat tttttgcttt tgccgagaat ttactaaaaa aaggaattat
     4141 taagcaatag gcgaaccaga agtaggaata catagctacg ctggaaaaaa atctgatcaa
     4201 accgcgctta ggttctcctg taggataaat agcatcccaa acgtttaaat tgtggatatt
     4261 gatttcatcc tgcctgttgc cgtttaaaaa aaaatcagga gattctatat tgaagatgcg
     4321 ctggccccaa ctgatttctt ctcccaggaa tataataaat attatgctta aaaaaagaaa
     4381 gaaaggattt ctttttctat taaaaatata ggggtgatct tctgattttg aatttaaaaa
     4441 tagatagaac agcagacttg ctgaaataag ataggaaaca gcagccaagg attctattat
     4501 gccatcctcc tgagtaagta atgccaacct ttccaggctt agactaaacg acaacaagtt
     4561 gtaagtaatt attattatta gtacaactaa tattggcaga tgactctgtt taaataaacg
     4621 gtgttttatc aggcttatgg ggagcattta tcattcaatt tggtgacaga tgtggtattc
     4681 atgaagctcg tctggcactc gtttaaaaaa aatatcttca caggctcgat cattatcata
     4741 cacggctttc aattatagaa ttaagggtag aaaggatgtt tgtttcaaat ttctcgagag
     4801 aataatattc cctaaatctt cggagcccat tctctcccat ggtgatccgt aatcttttat
     4861 tttcgattaa cctgattata ctctccgcaa gtttatgcgg attttctttt tcgaccagta
     4921 agcctgtttc gttttcaatt atcatatcag gaataccacc ctcacatgtt gccaccactg
     4981 gcaatccgca ctgcatagcc tcaaggatta caagtggaaa tgcttcgttc ctgctcggaa
     5041 gtacgaaaat atctgcttcc agtaatacat tgactttttc ctgaccatat agaggcccag
     5101 aaatttttat acaatctgcc aaatgcagtt tctcaacctg ctgtttcaga tcctctattg
     5161 acaggtcaat tggtgcgcct accagtttcg cgctgaatat gaatccctga tcatggatga
     5221 ttttgaatgc ttcgatgagg tctggaattc ctttctcaag catatagttt gaaatacata
     5281 ggatctgcga tatttctgac gaacttcgga ttggcttaag cttgttcgtc acgatgggct
     5341 caatgccatt tgggactatg tagggtttgc catcataaac gtcctcaatg tcataaagca
     5401 gtttttcgga aagacatatt accgaaatgt ctctgaagac agctttgtag agccttcttc
     5461 tcaggaaatt gttcctttga ttttccttga cgccttggcc atgaaggtgt atgacaagct
     5521 tcttcctgaa agatttaatt atgaatacat ataatgtatc cctatagaat gagtaaccct
     5581 taggcgaaaa gctaaaatat atcaggtcag gttttacggt gtgtatcttc caagccaact
     5641 caaaactgta agaaaaagct ttggctatct ttcttattga aaaccttgtg atctccttaa
     5701 ttgaagtaag aaactgcaga tttaagacaa tggtatcaaa attttctctg atcaccctgc
     5761 tgttcaccac attgctgttc atgacactgg ctccatgaac cgggggtggt aattgaacaa
     5821 taataaataa tttatttctg ttgtcggatg acattctaat gcaatgtgat ttaatgactc
     5881 cggattagct tcttacaggc cgaaattagt gaatttgcag aaacctttgc atcaaaaatc
     5941 cgtgcagatt ggattgaatt aaaagaaaat gtattataat ttgctagaat caccctgacc
     6001 ttctctgaaa agtcaacgga agaatatgct gattcgagaa ggaatccatt atatccatcc
     6061 ttcaaaactg acctaatatc accgacatca gtagcaatta caggagtccc gcaggtcagg
     6121 ctttctgcta tcaccattgg aaaaccctcc gatgaagagg ctattattag gattcgggaa
     6181 ttagctatca tcctgatagc agtgtcattg tcggtgaggc cgtgaaaagc cacattgtta
     6241 ttaagatcaa gattgtatac aagcttttga aggtgctttt cctggcttcc tgtcccaatg
     6301 atgttaagtt tattctgcgc ctttatgtcc gcaggcagct gactgtagag ttcaattatc
     6361 ttatcaacat tcttgacagt ttccagcctg cctgcataaa taaagtcttt tctgactgtt
     6421 ccggctaatc ctggcatgtc aatgctgacc ggattaaata gtttctctgc ttccggccga
     6481 aactcgccta cggtgaacag atgaccggct ttttttatga tcctggcttc aaaatgaccg
     6541 aacaggcgtc tgaaatactt accataccag aaagtggaca taatcaccgg attaaggttg
     6601 ccatggaaga tatgcataaa tggtatcctg aagaaggaca ctgcatatga tgcctccggc
     6661 gtgtgggata agactatgtc gaaattatgc cggtgcctga taaggaatag gaataccctg
     6721 atcagataag ccaaccttat tggcacaagc ttctgcttca gccagacaga ttgcagggta
     6781 acataggtca aggcaaactt ttgacgtagc cacggatcaa gattctttat gaagcttgcc
     6841 tgaccaccga aaccggaact aaggtcccgg tcattcagga tcagcatgct taccttctct
     6901 tgctgcgtac cattatccgt aagactgctc atcgcatgac acggtcactg acctgaattc
     6961 attcgagatt gaaatgcttc ttaataacct tctttttttc agcgtcggag aggtttttaa
     7021 ttttgaggaa tccgggattt tgtgtataca atagtttttg catgctcata gacattactg
     7081 tttttgccgg gtttccaatt attacagagt tctccgggaa ctggcctcgc acaacactgc
     7141 ctgcaccaat gacactgtta tctccgataa ctgtattggg tagaataata caattattgc
     7201 caataaaaac attattcccg atcttaattc tgccaaatac atcagcattt tcaatttcct
     7261 cccgaaggca ccagattgcc ccgtcatgtg tcagaaattc cgttcctcct gcaatggcta
     7321 catgattgcc aatctcgata agataaggtt cggtcgagaa ggccacggtg ttgataaaac
     7381 aattctcgcc aatcctaact ccctgttttc tgaaatagct aatcttcctt tgattgctgg
     7441 ctttccttaa taatgccagc cgaaactgca agaatttttg tttaacactc atttttgtgt
     7501 gttgatctga ttaaggagtt ctattcaatc tctccagcaa tgacatcagt aataaacttg
     7561 ccgaagagaa gggctgcttc tccggcaaat agctttcctg ggtgtgaatt gacaggactt
     7621 aaggcattct tatctttcag gtaaaggcct ccttcggttt caagttcata gaagtcccat
     7681 acaaatatgt tatcgccttt ttcgtcccat tcattgacta tccagtcccg gaactggcgg
     7741 gttcggacag cctcctcctc agtcatcagg tgtttggtat tgacagctgg tgtccatagg
     7801 atgaacctgg taccatggaa ctcatgcatt tttgtcttga gtgcagcata ctgagattgg
     7861 tagttttcaa cactttttat gtctgagtcc gcatccggac tactgatgtc agcttgtatc
     7921 aagctgacag gatagcaatg cttgaagata atgacgtcat attcttttgt cagaagttca
     7981 agtgttggtt cctcca
//
LOCUS       DAOSNB010000089         7991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9024_length_7991_cov_3.885207, whole genome shotgun sequence.
ACCESSION   DAOSNB010000089 DAOSNB010000000
VERSION     DAOSNB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9024_length_7991_cov_3.885207"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG42_05085"
     CDS             <1..237
                     /locus_tag="PLG42_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HOU30619.1"
                     /translation="YIGSHDHIFQHLKDSTSVIDYFVNTAGSSVRDVASNNMTVFAAS
                     SPGFSIISATKKDLSIYFIGIEGKALYKYIREKK"
     gene            364..1089
                     /locus_tag="PLG42_05090"
     CDS             364..1089
                     /locus_tag="PLG42_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU30620.1"
                     /translation="MENLYNLLTEDIRFVEGLKACLNCGVCTAICPAAEFYNYDPRKI
                     VNEVQKKDNEAIEALLKSETIWYCGECMSCKTRCPRGNTPGLIIMALRALSQKLGYFT
                     ESEKGRQQFAIKRTVGENIFKYGYCVATYTVKPELHPEQGPVWEFIIDHSREIYERLG
                     SKYDELGEGPMRKIPEDAKEELRKIFDVTGGTRFMNLIEEHSQRKAAELGYHTGEGIN
                     SEYFVKVYTENSGKHNQKEPGTL"
     gene            1086..2150
                     /locus_tag="PLG42_05095"
     CDS             1086..2150
                     /locus_tag="PLG42_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOU30621.1"
                     /translation="MKEPENKRIWSEYQKEIADDHFFYVRSCIRQSFFPGSEQFFLKM
                     MREILGKDIYENPSHTTCTGIGYHTDIVPFETIMTVIARHFALMKEAGYRNIAISCVT
                     SFGIYTEIIETWKHFPETLEKTREYLYKATGREFDVPENMAHTSDIVYKFRNEIAAKA
                     KYRLVNRFTGEPLKVVEHIGCHYSKMFPKYGVGGAEFPKVLSGMIEAWGGQVIDYPER
                     RHCCGFGFRQYIVQANRGYSLSNSVKKFESMKPYKPDFIITNCPGCNMFMDRWQYAMR
                     EMEGKTYGENGLSIPVLTYEEVAGLVLGINPWELGLQYHMVQSEPLLDKIGIEYNPSD
                     KYLTKDGIRIPAPENLINIK"
     gene            2154..3173
                     /locus_tag="PLG42_05100"
     CDS             2154..3173
                     /locus_tag="PLG42_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU30622.1"
                     /translation="MENIVVIGGGVSGLEAAGQLARMGYSVTVAEKNEKTGGNVKNWY
                     HLFPDRRDSKEVIDYLNKLASHPDIKILTGKEITEISSRNGKFDIKSSDGTVLEADAV
                     IVATGFELFDSRRKEEYGYGIYDNVITSADLEKMFRSGGVRLVNGKLPEKVGFIHCVG
                     SRDEKVGNYYCSKLCCVTAVKQAIEIKEKIPGAEIFCFYMDMRMGGPYYEELYREAQE
                     KYGITFIRGKVSEISVNINGKLVTKAEDTLAGRPLRMELDMVVLMVGTEMPEGNIKLA
                     ERSLLKCSENRFFKPVDNHYGSNLSGKKGIFYAGCCVAQMNITESISHARAAVSEVVN
                     YLKNR"
     gene            3175..3528
                     /locus_tag="PLG42_05105"
     CDS             3175..3528
                     /locus_tag="PLG42_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU30623.1"
                     /translation="MKSNKFGFTLSEPRVIDYEANNRQIAEYIFDHEPTFRLCIECGA
                     CAATCTAGQFMTFSLRELNVLLKRGENDIPRQRIKNCMFCGKCILVCPRGVNTRGVIF
                     HAREAFRKIEGNERF"
     gene            3515..5422
                     /locus_tag="PLG42_05110"
     CDS             3515..5422
                     /locus_tag="PLG42_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOU30624.1"
                     /translation="MKDFDPFVIPFNVGLYFILIYSVVRCVIWFRQLSRADKLRLQRG
                     FFSRAFGESLREIFMESLIHRKILKAKPVLGYMHMSLAFGWFLLIVFGTLEADIFGKS
                     HLNPPYKAIFFRFFNPGHGNSLLAEIWEFIMDLLLAFILSGLLLAIVKRFLPGVVGMK
                     KTTKLRLRDKIALTALWLVFPSRLIAESLTCGAHGSGSFLTGTTGAWLASFLPAESLA
                     YPFWWLYSLSLGVFFILLPVTRYMHIPTELFLIFMRNSGITTGDKAGTFAEVEVHSCS
                     SCGMCIDACQMSFVTGINNIQPVYLHRALRNNEDVTEKAYNCLMCGRCEEACPVGIEV
                     SAVRMIQRRNKTGKPLYENRWMGYFREHLNHEPASSGKTQNYKIRSNIRVEKADTIYF
                     AGCMTHLTPGIKEAMIKIFRAANEKVWFMDEVEGACCGRPLMLAGNDREARELINFNS
                     DLIWKSGASKLVTSCPICYNIFRSSYYIDTEILHHSQYIKNLIDNEAIRFNYSHKKVV
                     YHDPCELGRVAGIYEEPRDVVSWVADLVSVPEEAEKSLCCGNSLANIFLPPSKKNAIA
                     VDAIKRLTAGNPDIVATSCPLCKKTFSRVSKTRVADIAEIVAEEISSVQSKRIRTASV
                     NLREMVNQLLG"
     gene            5616..6125
                     /locus_tag="PLG42_05115"
     CDS             5616..6125
                     /locus_tag="PLG42_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30625.1"
                     /translation="MSFISLSSCSNKNKLSAESDTVVITQRDDGTISLDVEKATCYHN
                     RTDPSLNTAEWSIKITKPGRYGVWLVSAAKDTMNLGYRAPVTIDINGERLEVKPVGNK
                     IVRNAGNVKSPYYRADSYAGTFYVQNPGEYNVQVISEKIIALDNRKNWQNEPVNTMLM
                     SVMLTPMSR"
     gene            complement(6135..>7991)
                     /locus_tag="PLG42_05120"
     CDS             complement(6135..>7991)
                     /locus_tag="PLG42_05120"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOU30626.1"
                     /translation="QCAGKLQLPLNNLGAIALDYRGKAGMATSIGHAPVAGLPDPAAG
                     AILSIAEALTNIIWAPLKDGLKSVSLSANWMWPCKNPGEDARLYEAVKAASEFAIELG
                     INIPTGKDSLSMTQKYPDQTVYAPGTVIISAAGEVTDVSKIIEPVVSVDPYTSLIYID
                     MSRDSFKPGGSCLAQVLNCVGDEVPAINDAAYFRDVFNTVQSLIKSGNIISGHDISAG
                     GMITTLLEMCFANTEGGLSINLSAIDEPDTVKLLFSQNPGIIIQVKDEMEVSEILAEH
                     GISYYAIGHPVYERKLTVVNGEEKYEFDIDILRDTWFRTSYLLDRRQSGEKLAKERFE
                     NYKNHVINFSIPNFKGTFKALGITPTRRKKSGVRAAIIREKGVNGDREMAYALWLAGF
                     DVKDVHMTDLISGRETLEDVNMIVFVGGFSNSDVLGSAKGWAGAFRYNKKARTSLENY
                     YKRSDTLSLGVCNGCQLMAELGLIYPHHKEMPKLLHNASHKFESAFLGVKIPDNHSVM
                     LGNMSGMELGIWVAHGEGRFNLPYEEERYHVVLKYSRHTYPANPNGSDYDIAGIASED
                     GRHLAMMPHLERAFFPWQCGYYPEERKKDDITPWMQAFVNARKWIENKIKEK"
BASE COUNT         2333 a         1518 c         1842 g         2298 t
ORIGIN      
        1 tatattggtt cacacgacca tatcttccag catcttaaag attcgacttc tgtcattgat
       61 tattttgtta atacagccgg ttcttcagtg agggatgtgg caagtaataa catgacagtt
      121 tttgcagcat cctcccctgg tttcagcatc atttcagcta caaaaaagga cctttcaata
      181 tacttcatcg gaatcgaagg taaagccctc tataagtaca tcagggaaaa gaaatagctc
      241 atttgtgctt ctaatctaag tattaatacc tatttaaaaa taggttttaa ccctctttac
      301 atcctgttat tctctggtta tttttgtaaa taacctgaag attttgtctg aattatttaa
      361 ttgatggaga atctttataa tctgctcact gaagatatac gttttgttga agggctgaag
      421 gcgtgcctta actgtggtgt ttgcaccgcc atctgtccgg cagcagagtt ttacaattac
      481 gatccacgta agattgtaaa cgaagtccag aagaaagaca atgaagctat tgaagctctt
      541 ttgaagtctg aaacaatctg gtattgcggt gaatgcatgt catgcaagac acgttgcccg
      601 agggggaata cgcccggact tattattatg gctttaaggg ctctatcaca aaaactcggc
      661 tatttcacag aaagcgaaaa gggtcgtcag cagtttgcaa taaaaagaac tgttggtgag
      721 aatattttca aatatggtta ctgtgtggca acttatactg ttaagccgga gttgcatccg
      781 gaacaggggc ctgtttggga gtttatcatc gaccattcaa gggaaatata cgaacgactg
      841 ggctcaaaat acgatgagct gggcgaggga ccaatgagga agattcctga agatgcaaag
      901 gaagaacttc gtaagatttt cgatgtgaca ggaggaactc gctttatgaa tctcatcgag
      961 gaacattcac aaagaaaagc cgcagaactg ggatatcata caggcgaagg aataaacagc
     1021 gaatactttg tgaaagttta tactgaaaac agcggaaaac ataaccagaa agaacccgga
     1081 acattatgaa ggaacctgaa aacaaacgta tctggtcgga ataccagaag gagatagcag
     1141 atgaccactt tttctatgtc aggtcatgca tcagacagtc atttttccct ggctccgaac
     1201 agttctttct gaaaatgatg agagaaattc tcggaaaaga tatttatgaa aatccttcac
     1261 atacaacctg caccggtatt ggttaccata ccgacattgt tcctttcgag acaatcatga
     1321 ctgttattgc aaggcatttt gcactgatga aggaagctgg ttacaggaat atagcaattt
     1381 cgtgtgttac ttcctttggc atctacaccg aaataattga aacatggaaa cattttccag
     1441 aaactctgga aaagaccagg gaatatctgt acaaggctac gggcagggag ttcgatgtgc
     1501 ctgaaaatat ggcccataca agcgatattg tatataaatt cagaaatgag atagcggcaa
     1561 aagcaaaata caggcttgta aacagattta ccggggagcc tttaaaagtg gtagaacata
     1621 taggttgcca ctattctaag atgtttccaa aatacggcgt gggaggggct gaatttccca
     1681 aagttctctc tggcatgatt gaagcatggg gagggcaggt tattgattac cctgaacgcc
     1741 gtcactgttg cgggttcgga ttcagacaat acatagtaca ggcaaacagg ggctattcgc
     1801 tcagtaattc tgtcaagaaa tttgaatcta tgaagcccta caagccagat tttattataa
     1861 ccaactgtcc gggatgcaat atgttcatgg acagatggca gtatgcaatg agggagatgg
     1921 aagggaagac ttatggcgaa aacgggcttt caattccggt tcttacatat gaagaggttg
     1981 cgggattggt actgggtata aatccgtggg aactgggatt acagtatcat atggttcaat
     2041 cagagccgct tttggataaa attggtatag aatataatcc atctgataaa tacctgacca
     2101 aagatgggat acgcattcct gcgcctgaaa atctgataaa tattaaataa gcgatggaga
     2161 acattgttgt tataggcgga ggtgtttcag gtctggaggc agccggacag cttgccagga
     2221 tgggttacag tgttacagtt gctgaaaaga atgaaaagac aggaggaaat gttaagaact
     2281 ggtaccacct gtttcccgac aggcgcgaca gtaaagaagt tattgattat ctgaataagc
     2341 ttgcaagcca tcctgacatc aagatactta caggaaaaga aatcacagaa atatcttcaa
     2401 gaaacggaaa atttgacatt aaatcatccg acgggacggt tctggaagct gatgctgtaa
     2461 ttgttgcaac gggctttgaa ttatttgata gccggagaaa agaagagtac ggctatggca
     2521 tttatgataa tgttatcact tcagcagacc tggagaagat gtttagaagc ggaggtgtca
     2581 ggctggttaa cgggaagctg cctgaaaagg tcggtttcat tcattgcgtt ggctcgcgtg
     2641 atgaaaaggt gggaaattat tattgttcaa aactctgttg tgttactgct gttaagcagg
     2701 caatagagat taaggagaag attcccggtg cggagatttt ctgtttttat atggacatgc
     2761 gaatgggagg accatattat gaggaacttt acagggaggc gcaggagaaa tatggcataa
     2821 cttttatcag aggtaaagtt tcagagattt cggtaaatat caacggcaaa cttgtcacga
     2881 aggctgaaga tactcttgcc ggcagacctt taagaatgga actcgacatg gttgtactta
     2941 tggtgggaac agagatgcct gaaggcaaca ttaaacttgc tgaaagatca ctcctgaaat
     3001 gcagtgaaaa ccgttttttc aagccagtag ataatcacta cggaagcaac ctgagtggaa
     3061 aaaaagggat tttttatgcc ggttgctgcg tggctcaaat gaatatcact gaatcaatat
     3121 cgcatgcaag agctgctgtg agtgaggtgg ttaattatct gaaaaacaga tagtatgaaa
     3181 tccaacaagt tcggatttac tttatcagaa ccgagggtta tagactatga agccaacaac
     3241 aggcagatag ccgaatatat atttgatcac gaaccaactt tcaggctttg tattgagtgc
     3301 ggagcctgtg ccgctacatg tactgcagga caatttatga ctttcagcct gagggaactt
     3361 aatgtgttac tgaaaagggg tgaaaacgac atcccgcggc agagaataaa aaattgtatg
     3421 ttctgcggta aatgtatatt ggtttgtccg cgcggggtta acacacgagg tgttattttt
     3481 cacgcaaggg aagctttcag aaaaattgaa ggtaatgaaa gattttgatc catttgtgat
     3541 accttttaac gtaggcctat acttcatact tatctacagt gtggtgaggt gcgttatctg
     3601 gtttcgccag ctttcaagag ccgataaact cagactgcag aggggattct tcagcagagc
     3661 ctttggcgaa agcctgaggg agattttcat ggagagtttg atacatcgaa aaatattgaa
     3721 ggcaaaacct gtgctcggtt atatgcacat gagtcttgct ttcggatggt ttcttttaat
     3781 agtgtttgga acccttgagg cagatatatt cggcaaaagt catctcaatc ctccatataa
     3841 agctatcttt ttcagatttt tcaatcccgg tcacggaaat tcacttctgg cagaaatatg
     3901 ggagtttata atggatctgc ttcttgcctt tattctctcg ggcctattgc ttgctattgt
     3961 taaaaggttt ttgcctggtg tggttggcat gaagaagact acaaaactca gattgcgtga
     4021 taaaatagcg cttacagctc tctggctcgt ttttcccagc cgtctaattg ccgagagtct
     4081 tacctgtggt gcacatggtt cgggaagctt ccttacagga acaacaggtg catggttagc
     4141 atcttttctg ccagctgagt cactggctta tccattctgg tggctctatt cactctcact
     4201 gggagtattc tttattctac tgcctgtgac acgttatatg cacattccta ctgagctgtt
     4261 tcttattttt atgagaaact cgggaattac caccggtgat aaagcaggta catttgcaga
     4321 ggtggaggtt cattcatgtt cttcctgcgg aatgtgcatc gatgcctgtc agatgagctt
     4381 cgttacaggt ataaacaaca tacagcccgt ttatcttcac agagcattga ggaataatga
     4441 agatgtaacc gagaaggcat acaattgcct gatgtgcggc aggtgtgagg aagcatgtcc
     4501 agtcgggatt gaagtttcag ccgttagaat gatacaaaga agaaataaaa caggaaaacc
     4561 gctgtacgag aacagatgga tgggatactt ccgtgaacac cttaaccatg aacctgcatc
     4621 aagcggtaaa actcagaatt acaaaataag aagcaatata agagttgaaa aagctgatac
     4681 aatctatttt gcaggatgca tgactcatct tacaccaggg ataaaggaag cgatgattaa
     4741 gattttcagg gcagcaaatg aaaaagtatg gttcatggat gaggtagaag gtgcatgctg
     4801 cggaagaccc ctgatgctgg caggtaatga cagggaggca agggaattga taaactttaa
     4861 ttcggatctg atatggaaat caggtgcaag taaacttgtt acttcgtgtc ctatttgtta
     4921 taacattttc aggtcaagtt attacattga tactgagata cttcatcact cacaatatat
     4981 taagaacctt attgataacg aagcaatcag attcaattat tcacataaga aagttgttta
     5041 tcacgatccc tgtgaactgg gaagggttgc aggtatttat gaggaaccga gagatgttgt
     5101 ttcatgggtg gcagatcttg tttcagttcc cgaagaggct gaaaaatcgc tttgctgtgg
     5161 caattcactt gccaatatat ttcttcctcc ttcaaagaaa aacgcaattg cagtagatgc
     5221 cataaaaaga cttacagccg gaaatcctga tatcgttgcc acttcatgtc cactatgtaa
     5281 aaagaccttc tccagggttt caaaaacaag ggtggcagat atagcagaaa tagttgccga
     5341 agaaatttcc agtgttcaga gtaaaagaat cagaactgca tcagtgaatc tcagggagat
     5401 ggtcaatcaa ctccttgggt agaagcttcc atagtaaaac tgttcaattc acttaacaaa
     5461 atccaataaa aattttatta gatttacacc aaaatatttt tacctgttga tacgttaaaa
     5521 ttgtacagca aaaccgggtt aaaagaagta tgatattatt ttaattaatt gattattaac
     5581 acatgaaatt tggttggata aatttattgg taatattgtc ttttatatca ctgtcatcat
     5641 gcagcaataa aaataaactg tcggctgaaa gtgatactgt tgttataact cagcgtgatg
     5701 atggtactat atctcttgat gttgaaaagg cgacgtgtta tcataacagg acagatcctt
     5761 ctcttaatac tgccgaatgg agcataaaaa ttacaaaacc cgggcgttat ggagtgtggt
     5821 tggtgagtgc tgcaaaggac actatgaatc ttggttatag agctccggta acgattgata
     5881 ttaacgggga aaggctcgaa gttaagcctg ttgggaataa gattgtgagg aatgccggta
     5941 atgttaaatc tccttattac agagccgatt cgtatgccgg gactttctat gttcagaacc
     6001 cgggtgaata taatgtacag gttatcagtg agaaaataat tgccctggat aacagaaaaa
     6061 actggcagaa tgagccggtt aatacaatgc tgatgtcggt gatgcttact ccgatgtcaa
     6121 gatagcctga ggtgttattt ttcctttatt ttgttttcga tccactttct tgcattaaca
     6181 aacgcctgca tccatggggt tatatcgtct tttttcctct cttcggggta atagccgcat
     6241 tgccagggga agaaagctct ttcgaggtga ggcatcatag caagatggcg gccgtcttcc
     6301 gaagcgattc cggcaatatc ataatctgaa ccattgggat ttgcaggata agtatgcctg
     6361 ctgtatttca aaactacatg gtatctctct tcttcgtatg gaaggttgaa tcttccttca
     6421 ccgtgtgcta cccatatgcc gagttccata cccgacatat ttcccagcat aacagaatga
     6481 ttgtcgggga ttttaactcc caggaaggcc gattcaaact tatgtgatgc gttatgcaat
     6541 agttttggca tctctttgtg atgggggtaa ataagcccga gttctgccat aagctggcat
     6601 ccgttgcata cacccagcga aagagtgtcg gatcttttgt aataattttc gagtgaggta
     6661 cgtgcttttt tattgtatct gaatgcacct gcccatccct ttgcggagcc cagcacgtca
     6721 gagtttgaga acccacccac aaacacaatc atattgacat cttcaagtgt ctccctgcct
     6781 gatataaggt cggtcatgtg aacatctttc acatcgaagc cggcaagcca tagtgcataa
     6841 gccatctcac ggtcgccgtt tacacctttt tcccttatta tggcagctct tacgccgctt
     6901 ttcttgcgcc gtgttggggt tatacctaac gccttgaagg tgcctttaaa attgggtatg
     6961 ctgaagttga taacatggtt tttgtaattt tcaaacctct cctttgcaag tttttcacct
     7021 gactgccgtc tgtcgagcag ataagatgtt ctgaaccatg tgtcgcgtaa tatgtcgatg
     7081 tcaaactcgt atttttcttc tccgtttact actgtcagtt ttctttcata caccggatgt
     7141 cctatagcgt aatatgatat tccatgttca gccagtattt ctgatacctc catctcgtcc
     7201 tttacctgga tgatgattcc aggattctgg ctgaagagca gttttacagt gtcgggttca
     7261 tcaatagctg aaaggtttat cgacagtcct ccctcagtgt ttgcgaaaca catctccaga
     7321 agtgtagtta tcattccgcc tgcagagatg tcatggcccg atataatatt gcccgattta
     7381 atgagactct gaacggtgtt gaagacgtcc ctgaagtatg cagcatcgtt tattgcaggc
     7441 acttcatctc ccacacaatt aagcacttgt gccagacagc ttccgccagg tttgaaactg
     7501 tcgcggctca tgtcaatata aatgaggctg gtataaggat ctaccgatac gactggttca
     7561 ataattttac ttacatctgt aacttctcct gctgcagaaa tgattactgt accgggtgca
     7621 taaactgtct gatcgggata tttttgcgtc attgagaggc tgtccttgcc agtgggtatg
     7681 ttgattccca gttcaatggc aaactcactt gcagccttta ctgcttcgta gaggcgtgca
     7741 tcttctcccg ggtttttgca tggccacatc cagttggctg agagtgagac gcttttgaga
     7801 ccgtctttca gaggggccca gataatgttt gtcagagctt cggctattga aaggattgca
     7861 ccagccgcgg ggtcaggaag acctgcaaca ggtgcatgac ctattgaagt tgccatccct
     7921 gctttccccc tgtagtcgag tgcaatagct ccgaggttgt tgagaggcaa ctgaagcttt
     7981 cctgcgcact g
//
LOCUS       DAOSNB010000090         7971 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9051_length_7971_cov_4.464250, whole genome shotgun sequence.
ACCESSION   DAOSNB010000090 DAOSNB010000000
VERSION     DAOSNB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9051_length_7971_cov_4.464250"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /gene="ccsA"
                     /locus_tag="PLG42_05125"
     CDS             <1..310
                     /gene="ccsA"
                     /locus_tag="PLG42_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOU30627.1"
                     /translation="WDPKETWSLITIIVYSFVVHSRMMPSLKNIYAFNLLSVLAISSV
                     LMTYFGVNYYLSGLHSYAGGDPVPVPAFVYLAIAVIVALAILSYAKYKKWEMPASPNE
                     "
     gene            complement(527..602)
                     /locus_tag="PLG42_05130"
     tRNA            complement(527..602)
                     /locus_tag="PLG42_05130"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(565..567),aa:His,seq:gtg)
     gene            895..2241
                     /locus_tag="PLG42_05135"
     CDS             895..2241
                     /locus_tag="PLG42_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOU30628.1"
                     /translation="MKKIKFIDNISGLQLFQLLRFTVFLIISIVFTKSHLTKAEIGDW
                     EMFLFIASLVSFFWVTGIIQSLLPIYHRNRTYGKRGENIGEKSPEIFNAFLLLTFFSL
                     LVFLLGFSIKGSVSIFGFSGKDPFIRLLLWYILLSNPVCLIEYIYLLKNRAHRIVQYG
                     VYTFAAQLLMIIGPIVAGKDLFWAFYGLLAITAIRWVWLIVLLKRYSEMKVSFEFMKE
                     HLYLGAPLILTSLISGSAQYIDGIIISAYYKNPEIFAHFRYGAKEFPLVLMLANGLSN
                     AMLPEFSTRSRMKEALGKIKTRSRKLMHFLFPSSMVVMLFTRWLFPRVFTPEFIRSAD
                     IFLIYLLLIIPRLVFPQTIVIGRKKTHITLIAAVIELAINIPLSLLLIEPYGTVGVAL
                     ATFIVYCIEKIFLVWYVWTKMNINPSEYIPLKEYVTYSVLITILFVLIDHRIIDIQ"
     gene            2346..3575
                     /locus_tag="PLG42_05140"
     CDS             2346..3575
                     /locus_tag="PLG42_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30629.1"
                     /translation="MSFRVIEVKTKADRKAFLRFAKELYKGDPNWVCPLDDELEGVFD
                     PGRNPTFRHGEATRWILVDDKNKVIGRIAAFIDYIRSKANTQPTGGIGYFEVIDNRDA
                     AFMLFDTAREWLAQRGMEAMDGPINFGENESNWGLLVEGFMQQGIGMPYNKEYYRKFF
                     EEYGFQKYFEQYSYHCTVRGPDNKIVMFPERMVKIAEWISKRPGYSFQHVEFKNLEKY
                     INDLIEVYNSTWSSFKEDFTPLEPQFLKETFRKIRAFVDEELIWFAYYKDKPVAFFIL
                     YPDLNQIIKHFNGKLHLWNKLRFLWYKATHKMTRMRAVVGGVNPSHQNSGIESAIFLQ
                     LYNVFKRKKWYKELELSWVGDFNPKMIAIYEALGAKKAKTHYTYRYMINKNIPFVRYK
                     EEIAIKLDRRKDEGEKG"
     gene            3659..3925
                     /locus_tag="PLG42_05145"
     CDS             3659..3925
                     /locus_tag="PLG42_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOU30630.1"
                     /translation="MKKGIHPGNYRLVVFQDMSNGYAFLGKSTAPSKETIKWEDGKEY
                     PLIKLEISNTSHPFYTGKMKLVDSAGRVDRYMSRYKSHLEKKKQ"
     gene            4149..6563
                     /gene="lon"
                     /locus_tag="PLG42_05150"
     CDS             4149..6563
                     /gene="lon"
                     /locus_tag="PLG42_05150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOU30631.1"
                     /translation="MINGEGEIIPIIADGDDRDLEDIEVPEVVPVLSLRNTVLFPGVV
                     LPISIARPRSIQLIRDAYRTDKIVATVAQKDPDIENPAYEDLHTVGTLGQIIKLLEMP
                     DGSTTAIIQGRKRIKLETLVSSEPYFMARVKAAPEIKPEPDNREFETIVGSLRDMSLK
                     IIKLNPNISPEATFAIKNIENSFYLINFICSNTDIKMQDKQKLLEADSMRDRGYMLLQ
                     YLVEEIQMLELKTELQMKVKSDLEQQQREYLLHQQMKKIQDELGVNPLEKEIEEFKKK
                     ADQKKWSQDVRKIFDRELDKLYRLNPAAAEYSVQVNYIQTLLDLPWGEYTEDNLDLAN
                     ARKVLDDDHYGLEEVKERILEHLAVLKLKGDMKSPILCLYGPPGVGKTSLGKSIARAL
                     GRKYVRMSLGGLHDEAEIRGHRKTYIGAMPGGIIQNIKRAGSSNPVFILDEVDKVGQD
                     FRGDPSSALLEVLDPEQNNTFYDNFLEMEYDLSRVMFIATANTVATISPALRDRMELI
                     EITGYLPEEKVEIALRHLLPKQLENHGVKPDELKISRKLMEIIIDKYTRESGVRELDK
                     RLAKIVRIRAKDIASGTKFNPELTAEELTEILGPPKYTRDLYTGNEQAGVVTGLAWTA
                     TGGEILFVETSLSRGNGKLTLTGNLGEVMKESAVLALEYLKSNADLLGLPDNFAEKWN
                     IHIHVPEGAIPKDGPSAGITMAVSIASAFTQRKVKKYLAMTGEITLRGKVLPVGGIKE
                     KILAAKRASIREIVLSSDNRKDVEAIDKIYLKGLKLHYVDTIMEALEISLLKQKVKNP
                     KKITFD"
     gene            6566..7051
                     /gene="coaD"
                     /locus_tag="PLG42_05155"
     CDS             6566..7051
                     /gene="coaD"
                     /locus_tag="PLG42_05155"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOU30632.1"
                     /translation="MKKIAVFPGTFDPFTVGHEGVVKRGLEIFDEIIIAVGANPLKKC
                     LYSVETRKKMISEVFRNEPRIKVDHYEGLTVDYCRKHNAKFILRGLRTAADFEFERAI
                     AQMNRIMAPDIESVFILTIPEHAPVNSTIIRDIIKSGGDASMFVPSAIKLSDYKFDEE
                     S"
     gene            7038..>7971
                     /locus_tag="PLG42_05160"
     CDS             7038..>7971
                     /locus_tag="PLG42_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30633.1"
                     /translation="MRSLKIIFSLFLLFPLISSLAQPVIIKGNGEGYAGAKLAFYRQS
                     DPVSRKLIPLFITECNEKGEFQTEIPVKSIELVYIKSGIYRFLLLVREGHTYELKLPA
                     YNPMPPEEENNPFFAGITVIPEVVNNPGDINNLIRTFDAEYNTVFNRVSERVDKDVRK
                     DEIPGLIESLNKLTGYSDDPFFRDFVKYRTIMLNLVAWGEYPGRREDSVLINQKFVPE
                     NPAYTDLLEQLYENSFKLLSAGPLKKEFAGAVNNSSPSEIMKILTREGKVVNPLLQQY
                     VILMNTYYGYFSGLLKEENALAIFDSLHTGGKNEY"
BASE COUNT         2468 a         1569 c         1715 g         2219 t
ORIGIN      
        1 ctgggatccc aaggagacat ggtcgcttat aacaattatt gtttacagct ttgtagtaca
       61 ttcacgcatg atgccctctc tgaaaaatat ttatgcattc aatctactgt cggttctggc
      121 aatatcatca gttttgatga catattttgg agtaaattat tatctgtcgg gtctccattc
      181 atatgccgga ggagatcctg ttcctgttcc ggcgtttgtc tatttagcta ttgcagtgat
      241 agttgcactt gcgattcttt catatgcaaa atataaaaag tgggaaatgc ccgcttcacc
      301 caatgagtga ttgttatata aggccggcct ttcaggccca gcctttctga cgggtggaag
      361 accggattga ccaccttacg gtggtcgaac ctttttgaaa gttacttccg tcagaaattc
      421 tgttttgcct ttggcaaaat cggccttatt ttttaattac tcgcacaggc aagcctggct
      481 cgtttaaaaa aaataaggcc ggcctttcag gcccagcctt tctgacgggt ggaagaccgg
      541 attcgaaccg gcgacctcca gagccacaat ctggcgctct gaccaactaa gctacatcca
      601 ccgtatccgg cttgcaaaat tatatccaaa aagggagaat acaaaatttt caatattttt
      661 aacttgagcc tttaacccat tttttaactt tgccctgttt aaaaaataaa tgctggtgtt
      721 acaattttta atcattaccg aagttttatg ttaagtttca aaactccttt tgaactcata
      781 tttactttaa aaagaatcaa cagcttcatc tctgtaatga attggaagct ttgatggata
      841 aaggccagaa taaaggaacg agttcagggg aataatgatt atgcaacaaa aggtttgaag
      901 aagataaaat ttatagataa catttcgggg cttcagctct ttcaattgct gaggtttacg
      961 gtttttctga tcatcagcat agttttcacc aaaagccatc ttacaaaagc tgagattggt
     1021 gattgggaga tgtttctttt catagcaagc cttgtcagtt tcttctgggt gacgggaata
     1081 atccagtcgc ttcttcctat ttaccaccgt aaccgcacat atgggaaaag aggggaaaat
     1141 atcggtgaaa aatcacctga aatattcaat gcatttcttc tgcttacctt tttcagcctg
     1201 cttgtttttc tgctaggatt ttcaattaaa ggctctgtct ctattttcgg tttttcgggt
     1261 aaagatccat tcatcaggtt actactctgg tacatacttc taagcaatcc agtatgcctc
     1321 attgaatata tctatcttct caagaacaga gcccaccgta tagttcagta tggtgtttat
     1381 accttcgcag cccagctgtt aatgataata ggcccaatcg ttgcagggaa ggaccttttc
     1441 tgggcatttt acgggctgct tgccataacg gcaataagat gggtatggct gatagttctt
     1501 ttaaaaaggt actcggagat gaaggtttca tttgaattta tgaaggaaca cctttatctc
     1561 ggagcacctc tgatactaac ttcgcttata agtggttcgg cacagtatat cgacggaata
     1621 atcatctcgg cgtattataa gaatccggaa atctttgcac acttccgata tggtgcaaaa
     1681 gagtttccgc ttgttctgat gcttgcaaac gggttgagca atgcaatgct gccggagttt
     1741 tcaaccaggt cgcgaatgaa agaggcactt ggaaaaatta aaaccaggtc aagaaaattg
     1801 atgcattttc ttttcccttc aagcatggtg gtgatgctgt tcacccgctg gctcttcccg
     1861 cgagtattca cacccgaatt cataagaagt gctgatattt tccttattta cctgctgctt
     1921 ataatacctc gcctggtatt cccccagaca atcgtgatag gccgaaaaaa gactcatatt
     1981 acacttatcg ctgcagtaat agagctggca ataaatatac cactgagcct cctgcttatt
     2041 gagccttatg gaacagtggg agtagcactg gcaaccttta tagtatactg tatcgaaaaa
     2101 atattccttg tgtggtatgt atggacaaaa atgaatatta acccatcaga atacatacct
     2161 cttaaggagt atgtgacata ttccgttctg attacaatcc tctttgttct gattgaccac
     2221 agaattatag atattcaata ataagctaac tcgtaatatt taacttttat ttgcatatct
     2281 ttgtgtcagt ctttacggta atttccgaac tgtgaagtca atataatttt tgaaccacag
     2341 gtaatatgtc cttcagggtt atagaagtca agacaaaagc tgacaggaaa gctttcctgc
     2401 gttttgctaa agagctatat aaaggtgacc ccaactgggt ttgtcctctc gacgatgaat
     2461 tagagggagt ttttgatccc ggaagaaatc ccacctttcg tcatggagaa gccacaaggt
     2521 ggattcttgt tgatgacaaa aataaagtta tcggcaggat tgcagctttt atagattata
     2581 taagatctaa ggcaaacacg cagccgacag gcggaatagg ctattttgag gtgattgaca
     2641 accgtgatgc cgccttcatg ctgttcgaca ccgcaagaga gtggcttgca cagagaggta
     2701 tggaagcaat ggatggcccc attaattttg gtgaaaatga gagtaactgg ggtctgctcg
     2761 ttgagggctt catgcaacag ggcatcggta tgccttataa taaagaatat tacaggaaat
     2821 tctttgagga gtacggattc cagaaatatt ttgagcagta ttcataccat tgtaccgtaa
     2881 gagggccgga caataagatt gtgatgtttc cggaaagaat ggtaaaaata gcggagtgga
     2941 tttcaaaaag gccgggatac tcctttcaac atgtggaatt caaaaacctc gaaaagtata
     3001 ttaacgacct tatagaagta tataattcaa catggtcctc attcaaggaa gattttacac
     3061 ctcttgagcc tcagttcctg aaggaaacat tccgcaagat cagagcattt gtggatgaag
     3121 aacttatatg gtttgcctat tacaaagaca aacctgtggc attcttcatt ctctatcccg
     3181 acctcaacca gataataaaa cacttcaacg gcaaacttca cctttggaat aaattgcgct
     3241 ttctgtggta taaagctacc cataaaatga cacgcatgag agctgtggta ggtggtgtta
     3301 acccgtcaca ccagaacagc ggaattgaat cggcaatttt ccttcagctt tataatgtgt
     3361 ttaaaaggaa aaagtggtac aaggaacttg agctctcctg ggtgggtgat tttaacccga
     3421 agatgattgc catttacgag gctctcggag caaaaaaggc aaagacacac tatacctacc
     3481 ggtatatgat aaataaaaat atccctttcg tcaggtataa agaagaaatt gcaattaaac
     3541 tggaccgtag aaaggatgag ggagaaaaag ggtgatataa attattttca atgatgtttt
     3601 ttatttaaaa ataaataagt aaatttgcaa ccctgtttta ataattaata tttgaaagat
     3661 gaagaaaggt atacatccgg gtaattacag gcttgttgtg tttcaggaca tgtcgaacgg
     3721 ctatgctttt ctgggtaaat caacagcccc ctcgaaggaa accattaaat gggaagacgg
     3781 aaaggaatac ccattgatta aacttgagat atcaaatact tcccatccgt tctatactgg
     3841 taagatgaaa cttgttgact ctgcaggtcg cgtcgacagg tacatgagcc gctataaaag
     3901 ccatcttgaa aagaagaaac aataatagtt tttacgataa aataaaaagg gggctgacat
     3961 tacctgttag cccctttttt acgtttttta caacgggaaa gtctggtata aattttgaag
     4021 gtgttgataa cctcgattct ataatggtta attgccagaa aaataatatg taattttaaa
     4081 cgtactttta aaactatatg gacgaaaagc aaaaaaagtc gctgatacag ggttttttct
     4141 ttcaggagat gattaatggg gaaggagaga taattcctat tattgccgac ggagacgaca
     4201 gggatcttga agatatagag gtgcccgagg tggtgcctgt actttcatta cgtaatacag
     4261 ttctttttcc aggcgttgtt ctgcctattt ccatagcgcg tccgcgttcc atccagctta
     4321 taagggatgc ataccgcacc gataagattg ttgcaactgt ggcacagaaa gatcccgata
     4381 tcgaaaatcc tgcctatgaa gaccttcata ccgttggtac gctcggccag ataatcaaac
     4441 tactcgagat gcccgacggt tcaaccacag caataataca gggcagaaaa agaataaagc
     4501 ttgagacgct tgtctcatcc gaaccttatt ttatggcaag ggttaaagct gctcctgaaa
     4561 ttaaaccgga acctgataac agggaattcg aaaccatagt cggttccctt cgtgacatgt
     4621 cgcttaaaat tatcaagctt aatcccaata tttcacccga ggcaacattc gctataaaaa
     4681 atatagagaa cagtttctac ctgattaatt tcatctgctc caataccgat ataaagatgc
     4741 aggataagca gaaactcctt gaagctgata gtatgcgcga caggggttat atgctgcttc
     4801 aatatcttgt ggaggagatc cagatgctgg agctcaaaac agagcttcag atgaaggtta
     4861 aaagcgatct cgaacagcaa cagagggaat atctgcttca tcagcagatg aagaaaatac
     4921 aggatgaact gggagtaaac ccgcttgaga aggagataga agagtttaaa aagaaggcag
     4981 atcagaaaaa atggtcacag gatgtacgga aaatattcga cagggaactt gataaactct
     5041 accgcctcaa ccctgctgcc gctgagtatt ctgtacaggt gaactatatc cagacactcc
     5101 ttgaccttcc atggggtgaa tataccgaag ataaccttga tcttgccaat gcccgcaagg
     5161 ttcttgacga cgaccattac gggcttgagg aagtaaagga aagaattctc gaacatctgg
     5221 cagtgcttaa acttaaaggt gacatgaagt cgcctatact gtgtctttat ggccctccgg
     5281 gtgtaggaaa gacctcactt ggcaaatcaa tagcaagggc actgggccgc aaatatgtca
     5341 gaatgtcact tggagggttg cacgatgaag cagagataag agggcatcgc aagacataca
     5401 taggagcaat gcccggcggt ataatacaga acataaaacg tgcaggctcc tcaaacccgg
     5461 ttttcatcct cgatgaggtc gataaagttg gtcaggattt cagaggcgac ccttcatctg
     5521 ccctactgga agtccttgac cctgagcaga ataacacttt ctacgacaac ttcctcgaaa
     5581 tggaatatga cctttcgaga gtcatgttca tcgctacagc caatactgta gctactataa
     5641 gccctgccct gcgcgacagg atggaactta ttgaaataac cggatacctg cctgaagaga
     5701 aagttgaaat tgccctgcga catcttctgc caaaacagct cgaaaatcac ggtgttaaac
     5761 ccgatgaact gaaaataagc aggaaactga tggagattat tattgataag tataccaggg
     5821 aatcgggcgt aagggaactt gataaacgtc tggccaagat tgtgagaatc agggcaaaag
     5881 atattgcttc tggtacaaaa ttcaatccgg agctgaccgc agaagagctg actgaaatac
     5941 ttggtccgcc caaatatacc cgggatctct atacaggcaa tgaacaggca ggtgttgtca
     6001 ctggccttgc atggaccgct accggcgggg agatattatt tgttgaaaca agcctcagca
     6061 ggggtaacgg taaacttact cttacaggaa accttggcga ggtaatgaaa gaatcagcag
     6121 tgcttgcact tgaatacctt aaatcgaatg ctgatttgct gggtctgccc gataacttcg
     6181 ctgaaaagtg gaacatccat attcatgtgc ctgagggagc aatacctaaa gacggccctt
     6241 cagcaggtat taccatggct gtttcaatag cttcagcatt cacccagagg aaggtaaaga
     6301 aataccttgc catgaccggt gagataaccc tgcgcggaaa agtacttccg gtgggcggca
     6361 tcaaggaaaa aattctcgca gcaaaaagag caagcataag agaaatagtt ctttcatcag
     6421 ataacagaaa agatgtggaa gctatcgata aaatctacct gaaaggactt aaattgcatt
     6481 atgtcgacac cataatggaa gccctcgaaa tatcgcttct taaacaaaag gtgaaaaatc
     6541 cgaaaaaaat aacctttgac tgaatatgaa aaaaattgct gttttccccg gaacttttga
     6601 cccctttaca gttggacacg aaggtgttgt aaaacgtgga ctcgaaatct tcgatgaaat
     6661 aatcatagca gttggtgcca acccgctgaa gaaatgcctc tattcggttg aaacaaggaa
     6721 aaaaatgata tctgaagtct tccggaatga gccaaggata aaggtagacc attacgaggg
     6781 actaacagtg gattactgca ggaaacacaa tgcaaaattt attctgagag gtttgcgaac
     6841 agcggccgat tttgaattcg aacgtgctat agcccagatg aaccgtataa tggcacctga
     6901 tatcgaatcg gtattcatac ttaccattcc cgaacatgca cctgtaaatt caacaataat
     6961 cagggatatc ataaaaagtg gcggagatgc ttcgatgttt gtgccttcgg ccataaaact
     7021 gtcagattat aaatttgatg aggagtctta aaattatttt cagtttattt ctgctttttc
     7081 ctcttataag tagccttgcc cagcctgtaa tcattaaagg caatggtgaa ggttatgcag
     7141 gtgcaaagct tgcattttac cggcagtccg atcctgtctc acgtaaactc attccgcttt
     7201 ttattactga atgcaacgaa aaaggggaat ttcagaccga aattccagtt aaatccattg
     7261 agttggttta tattaaatca ggcatttacc gttttttgct tcttgtccgt gaagggcata
     7321 cttatgaact gaaactgccg gcttacaatc caatgcctcc ggaagaagaa aacaacccgt
     7381 tttttgcagg aataaccgta attcctgagg ttgtgaataa tcccggtgat atcaataacc
     7441 ttataaggac atttgatgcg gagtataata ctgtattcaa cagagtctcg gaaagagttg
     7501 ataaagatgt gaggaaagat gaaattccgg gtttgattga aagcctgaat aaattgacgg
     7561 gttattcgga tgatcccttt ttcagggact ttgtaaaata cagaaccatt atgcttaatc
     7621 tggtagcatg gggtgaatat ccgggtcgcc gtgaagactc ggtacttatc aaccagaaat
     7681 ttgtgcctga aaacccagct tacaccgatc ttcttgaaca gttgtatgag aactcgttta
     7741 agttactttc tgccggaccg ttgaaaaaag agtttgcggg agctgtaaac aattcatcgc
     7801 cctcagaaat tatgaaaata ttgacccgag aggggaaagt tgtaaacccc cttctgcagc
     7861 agtatgttat tcttatgaat acatattacg gttatttcag cggtttatta aaagaagaaa
     7921 atgcccttgc aatttttgac tcacttcata cgggtggtaa aaacgagtat a
//
LOCUS       DAOSNB010000091         7954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9078_length_7954_cov_4.037220, whole genome shotgun sequence.
ACCESSION   DAOSNB010000091 DAOSNB010000000
VERSION     DAOSNB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9078_length_7954_cov_4.037220"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..2205
                     /locus_tag="PLG42_05165"
     CDS             424..2205
                     /locus_tag="PLG42_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU30634.1"
                     /translation="MKLKTIVVVVSLALLIAFCTRQRVNYEITSEELQEHINYLASEL
                     LKGRLTGSAGDSLAAYYIRDRLKNYGLKPLFDEGFQRFEIIVSVEPGKNNFLKIRDKE
                     FTSGEDFLPASFSDNGSLKAEVVFAGYGFNINTGSIKRNDYSGIDVKGKWVMVLRDIP
                     DAGNPSDEMVAFSSDRSKVLMAKDLGAAGVILVSGEAFDKNDILEPLTVTDSPAGIPV
                     IRVKRNIADIILENSGHNISSLEKKLSESGKMVSFTTGIEISAGTEIIKHKGITRNVV
                     MVLPGEDPALKEEYIIIGAHFDHLGSGGKNSSSRMPDTVAVHYGADDNASGVAMLIEL
                     AGKLAGTKGSHKRSIVFVAFTGEEKGLLGSKYFTENPPVELAKINAMINLDMVGRLKE
                     TRSMQVGGTGTAQNLEEKISHLVDTNFIKPVYTPEGSGPSDHSSFYAKNIPVLFFTTG
                     AHIDYHTPGDTPDKINYEGMISVSDYIFRIDSLLACDTPRLAFREAGPVNNISRMPGR
                     RGVTLGIMPDITGSVKNGLRADLVTPGKPADLGGMKKGDIITAINGKPVSNIEDYMFR
                     LSQLKRGDAITVEVLRDGKKIELLIQL"
     gene            2210..3100
                     /locus_tag="PLG42_05170"
     CDS             2210..3100
                     /locus_tag="PLG42_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30635.1"
                     /translation="MKKRIKNILVWLIAIIITLSAAIYQRLTGPTYPARAEITANGKL
                     YKLKLVRSLGLDEKPVVRLAIYDSTVSAKLFYKRFRTTEPYTETNFRYMVIPVHSFVM
                     NKIFGISEIKGFFASLPQQPPAGKLQYYIEITDSNGKLTLYKANPVVVRFKGSVPGWI
                     LIPHIMLMFIAMLFSTAAGIMSVVKHPSYKKYSVWTLVLLTAGGMILGPVVQHYAFGE
                     YWTGIPFGWDLTDNKTLIAFIFWIIAVIANRKKERPVYSALASFILLLVYSIPHSMLG
                     SELDYESGKIIQGSIINFLY"
     gene            3208..3774
                     /locus_tag="PLG42_05175"
     CDS             3208..3774
                     /locus_tag="PLG42_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU30636.1"
                     /translation="MENNVNATYRNIHQDLIEGCKNGDQKAQFQIYKLYYKAMYNTSL
                     RIVNDTMEAEDIMQESFLSAFEKIDTYSGTVSFGAWLKRIVINRSLDALNRRKMIFED
                     IEAYQGIRDTSSAETMYVDDIDSKVEEVKKAIERLPDGYRIILSLYLIEGYDHDEIAE
                     ILSISSSTSRSQLSRAKQKLLSELKKNQ"
     gene            3787..4350
                     /locus_tag="PLG42_05180"
     CDS             3787..4350
                     /locus_tag="PLG42_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30637.1"
                     /translation="MKNIEDILRENRNLFDDREPAEGHFERFEWKLERRLHSQARRRS
                     IVPYLLKAAVVTVLITLSSLWVWDNFIRTGKDRMALGDVSPEYREVENYYVRQVKSME
                     DELGTYYLSDNPEQKKMLEQEIKSMDSVYVQLQKDLKANPNDERIINAMIEHYQAKVE
                     VLSYIINQLKSIRNQNQKPQKNESSNI"
     gene            4331..5410
                     /locus_tag="PLG42_05185"
     CDS             4331..5410
                     /locus_tag="PLG42_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOU30638.1"
                     /translation="MKAPIFKRWLLVITAISLCALPAISQEVTKEFKKEYTANKNTTV
                     EISNRYGNVVVTSWDSDKVDIYVKITIEMPDKSRAEKLINLINVEFTESDNRISARTV
                     IDDKFSFSGWGTPSRKFSINYTVKMPYYCGLDLANRYGNTDIDEIRGLVNLDIKYGNL
                     TVTKLTREDEKPINKISISYGKGMIKEAGWLDLYLRYTTAFEMPLAKAIVIDSKYSKL
                     NVGEISSLVAVSGYDNYRIDKINNMVIESSYSTINVERLAKKLNLEASYGSVNVNYVP
                     EGFESINIDTRYTGVRIGIDESVSYKLEGKASYGSIKVDEDKFNVKKRIIGNNSSELE
                     GIVGENPSPQAEVKVNSSYGTVRLF"
     gene            5543..5821
                     /locus_tag="PLG42_05190"
     CDS             5543..5821
                     /locus_tag="PLG42_05190"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOU30639.1"
                     /translation="MITDMIHVEKPSEEKLKQLGVHSWPVWTKEESEFPWEYDEKETC
                     YILEGDVTVTPESGEPVRFGKGDLVVFPEGLKCRWKIHKAVKKHYRFG"
     gene            5826..7304
                     /locus_tag="PLG42_05195"
     CDS             5826..7304
                     /locus_tag="PLG42_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOU30640.1"
                     /translation="MRIADIAIFFIYLAGIILFGSSFYRKNRNATAFTLGNRNMPTWI
                     VGMSIFATFVSSISYLALPGQAYQTNWNPFVFSLSLPFAAYMAVKFFVPLYRSVNSPS
                     AYTYLEMRFGKWARIYASVMYLLTQLMRTGTILYLLAMMLNTAIGWDTATIIIITGIS
                     IMIYSVLGGIQAVLWTDAIQGIILIAGAVVCSVLLLFSMPEGPGQLFQIASLHDKFSL
                     GSFKPDFTTSTFWVVFIYGIFINLQNYGIDQNYIQRYLTTRTDSEARKSALFGSLIYI
                     PVSLLFVFIGTALFSFYTAMPELLEAGIAGDRVFPYFIFHQLPAGATGLLISSIFAAG
                     MSTISTSINSSATVIVTDYFRLSPDVKEHEKKSMKILYISSVLFSLSGILVGIAMINV
                     QSALDAWWKLASVFSGGMLGLFLLGFFSKKVNNAAAVAGVITGIIVIAWISLSPVLFK
                     GSLQKFACPLHGYLSIVLGTTAIFITGFTIGIIIDRTRKNRK"
     gene            complement(7315..>7954)
                     /locus_tag="PLG42_05200"
     CDS             complement(7315..>7954)
                     /locus_tag="PLG42_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOU30641.1"
                     /translation="KEKTKMAREELEKFRKEVASEVASASDTQLSIIDEKVKQIKRKV
                     KKTEKPIEKPEEQGLRVGDAVRMADTMAAGEIVEIRDEMATVETGSFRFQVSLEKLER
                     ISRAELKKSLRQGQVVMQADPVISQRKMSFKPEIDIRGVRGEEAISIVRNFIDDAIMV
                     QHRHLRIIHGKGNGILRQLIRQYLSTVDVVKSFSDEHVERGGAGITLVELDL"
BASE COUNT         2467 a         1571 c         1675 g         2241 t
ORIGIN      
        1 ggaaggtgaa gttgattttt gttctttcca ggaccccctt ccctgccttc ccccaagggg
       61 gaaggtgaag tggtttctgt tctttccagg acccccttcc ctgccttccc ccagggggga
      121 aggtgaagtt gatttttgtt ctttccagga cccccttccc tgccttcccc caagggggaa
      181 ggtgaagtgg tttctgttct ttccaggacc cccttccctg ccttccccca ggggggaagg
      241 tgaagttgat ttttgttctt tccaggaccc ccttccctgc cttcccccag gggggaaggt
      301 gaaatggttt tagagtttaa aataattacc agattattat taacaatatt aaaagagagc
      361 caaacagaaa aagatattaa ttttgactca ttcaaatgaa aatatatttt actttttgca
      421 gggatgaaat taaaaaccat tgtagttgtt gtcagtctgg cattattaat tgccttttgc
      481 acccggcagc gggtgaatta tgaaattacc agtgaagagc ttcaggaaca tattaattat
      541 ctcgcctcag agctgctcaa aggaagactt accggctctg caggcgatag tctggcggct
      601 tattatatca gagaccgttt aaaaaattat ggtctgaaac ctctttttga tgagggattt
      661 caaagatttg aaatcattgt ctcagtcgaa cccggaaaaa ataactttct aaaaatcaga
      721 gacaaagaat ttacctccgg tgaagatttc ttgcctgcat cattcagtga taatggttcg
      781 ctgaaagctg aagtagtttt tgccggatat ggattcaata ttaacactgg ttccatcaaa
      841 agaaacgatt actcaggcat tgatgttaaa ggcaaatggg tgatggtatt aagagatatc
      901 cctgatgccg gaaatccttc agatgaaatg gtagctttta gcagtgacag gagtaaagta
      961 ttaatggcta aagaccttgg agctgcaggt gtgatactgg tttcgggaga agcttttgat
     1021 aagaatgata tcctagaacc cctcacagta acagactcac cggcaggcat accggtaata
     1081 agagtcaaaa ggaatattgc agatataatt cttgaaaatt cagggcataa catatcatct
     1141 ctcgagaaaa agctcagcga atcgggcaaa atggtaagtt ttacgacagg tatagaaatt
     1201 agtgcaggaa ctgaaataat aaaacacaaa ggaatcaccc ggaatgttgt gatggttctt
     1261 cccggcgaag atcctgcctt aaaggaagaa tatataatta taggtgctca ttttgaccat
     1321 cttggttcag ggggtaaaaa ttcctcaagt cgcatgcccg acactgtagc tgttcattac
     1381 ggagccgacg acaatgcctc gggcgttgcg atgcttattg aactggcagg caagttagca
     1441 ggtactaaag gaagccacaa aagaagtatc gtatttgttg cattcacagg agaggagaaa
     1501 gggctgctgg gttcaaagta ttttaccgaa aatccgccag ttgaacttgc aaagatcaat
     1561 gcaatgatta atcttgacat ggtaggaagg cttaaggaaa cccgtagcat gcaggtggga
     1621 ggtacaggaa ccgctcagaa tctggaagaa aagatcagcc atcttgttga tacaaacttc
     1681 attaaacctg tttatacgcc tgaaggttcg ggaccttctg accactcttc attttatgca
     1741 aaaaatattc ctgttctatt ctttacaaca ggtgcccata ttgattatca tacacctggc
     1801 gacaccccgg ataaaattaa ctacgaaggc atgataagcg tgtcggatta tattttcaga
     1861 atagactcac tgctggcatg cgataccccc aggcttgctt tccgtgaggc tggtccggta
     1921 aataatattt ccagaatgcc gggacgaaga ggtgtaacgc ttggaataat gcctgatata
     1981 acagggagtg tgaaaaacgg actcagagcc gacctcgtta cacctggtaa acctgccgac
     2041 cttgggggta tgaaaaaagg agacataatt accgccatca acggtaaacc tgtcagtaac
     2101 attgaagatt acatgttccg cctgagtcag ctgaaacgtg gcgatgcaat aactgttgaa
     2161 gtactaagag acggtaaaaa aattgaactt ttgatacagc tgtaatacaa tgaaaaaacg
     2221 cattaaaaat attctggtct ggctgatagc aataataatt acactttcgg cagcaattta
     2281 tcaacgtctt actggtccca cctaccctgc cagagctgag ataacagcta atggcaaact
     2341 ttataaatta aaactggtca gaagtctggg acttgatgaa aaacccgtag tcaggctggc
     2401 aatatacgac tcaactgtat cggcaaaact cttttataaa agattcagaa ccacggagcc
     2461 ttacactgaa acgaacttca ggtacatggt aataccagtt cattcatttg taatgaacaa
     2521 aatcttcggt atcagcgaga tcaaaggctt ttttgcttcc ctgccccagc aacctccagc
     2581 tggaaagctg caatattaca ttgaaataac agacagcaac ggaaagctta cactatataa
     2641 agccaaccct gtggtggtga ggttcaaagg ttccgttccc gggtggatcc tgatacctca
     2701 tataatgctg atgtttatag cgatgctttt ctccacagca gcaggaatta tgagtgtggt
     2761 taaacaccca tcatataaaa aatattcggt atggacactt gtcttgctaa ctgctggcgg
     2821 aatgatactt ggacctgttg ttcagcatta cgctttcggt gaatattgga cggggattcc
     2881 atttggatgg gatcttactg acaataagac tctgattgct tttattttct ggattattgc
     2941 agttattgca aacagaaaaa aggagcgccc tgtttattca gccctcgcct cgtttattct
     3001 gcttcttgta tactcaattc cccacagcat gcttggttcg gaactggatt atgaaagcgg
     3061 caaaattatt cagggcagta tcataaattt tttatattaa tttaatgata tgcttatgca
     3121 tcacacatat tttttgtccg tctttatgtc agaaaaagac aggaagtgga tgcagcctta
     3181 catatcagcc ccagattcaa caataaaatg gagaataatg taaacgcaac atacaggaac
     3241 atccaccagg atcttatcga agggtgtaaa aacggggatc aaaaggctca gtttcagatc
     3301 tacaagcttt actacaaagc gatgtacaac acgagcctga ggatcgttaa cgacacaatg
     3361 gaagctgagg atataatgca ggaatctttc ctgtcggcat ttgaaaagat tgacacatac
     3421 tcaggaacag taagtttcgg ggcatggctg aagagaatag taattaacag gtcgcttgac
     3481 gccctgaaca gaaggaagat gatctttgaa gatattgagg cataccaggg aataagagac
     3541 accagcagcg cagaaacgat gtatgtggat gatatagatt cgaaggtgga agaggttaag
     3601 aaagcgatag aaagactgcc tgatggttac agaatcattc tttcgcttta tctgattgaa
     3661 gggtacgacc atgatgaaat agctgaaata ctttcgattt caagcagtac atcaaggtcg
     3721 cagctttcaa gagcaaaaca aaaactcctg agcgagttaa agaaaaacca gtaaaaaatt
     3781 atgaaaatga aaaatataga agacatactc agagaaaaca gaaatctgtt tgacgacaga
     3841 gaacctgctg aagggcactt tgaaagattt gaatggaagc tcgaaagacg actccattcc
     3901 caggcacgaa gaaggagtat tgttccgtat ctgcttaaag ctgctgtggt tactgtactt
     3961 atcacactgt cgtcactgtg ggtttgggat aactttatcc gtaccggtaa agacaggatg
     4021 gctcttggcg atgtttcgcc cgaatacagg gaggtcgaaa actactacgt tcgtcaggtt
     4081 aaatcaatgg aagatgaact cggcacatat tacctcagcg acaatccaga gcaaaagaag
     4141 atgcttgaac aggaaataaa aagcatggat tcggtttatg tccagctcca gaaagatctt
     4201 aaagctaatc ctaacgatga gagaattata aatgccatga ttgaacatta ccaggcaaag
     4261 gtggaagtgt tatcttatat tatcaaccag ctgaaatcaa ttagaaatca aaaccaaaaa
     4321 ccacaaaaaa atgaaagctc caatatttaa aagatggttg ctggttatta cagcaataag
     4381 cctttgtgca ctacctgcca tttcgcagga agttacaaag gaattcaaaa aggaatacac
     4441 agcaaacaaa aatacaactg ttgaaatcag caacagatac ggtaatgttg ttgttacaag
     4501 ctgggatagt gataaagtgg atatttatgt taaaataacc atagagatgc ccgacaaaag
     4561 cagggcagaa aaactaatta atctcatcaa tgttgaattc actgaaagtg ataaccgaat
     4621 atctgccaga acagttattg acgacaaatt cagtttctcg ggatggggta ccccttcaag
     4681 aaaattcagc ataaactata cggtaaaaat gccctattat tgcggacttg accttgccaa
     4741 caggtatggc aatactgata ttgacgaaat aagaggtctc gtaaatctgg atatcaaata
     4801 cgggaacctt accgttacaa aactcacaag agaggatgaa aagccgataa acaaaatatc
     4861 aatttcttat ggtaaaggta tgataaagga agccggatgg ctagatctat acctcaggta
     4921 tactacagca ttcgaaatgc ctctggcaaa ggcaatagtt atcgatagca aatattcaaa
     4981 attaaatgtt ggtgaaatta gttcactggt agctgtatcg ggatatgaca attacagaat
     5041 tgataaaata aacaacatgg tgattgagtc gagctattca acaataaacg tagagagact
     5101 tgccaaaaaa ctcaatctcg aagcaagcta cggctcagta aatgtaaatt atgtgccaga
     5161 aggttttgaa tcaataaata ttgataccag atataccggg gtgagaatag gcatcgacga
     5221 gtcagtaagc tacaagcttg aaggaaaagc ctcttacggt tcaatcaaag ttgatgaaga
     5281 taaattcaat gtcaaaaaaa gaattatcgg aaataattct tcagaactcg aaggaattgt
     5341 tggggaaaat ccatcaccac aggcagaagt taaagtaaat tcttcatatg ggacagtaag
     5401 actcttctag ttatgaatta atagaagaaa tcaaaaaacg agatagtgtt tttccattat
     5461 atcttttaac atcaaaagaa tctgattctt cccttcattt atttaaaatt tcaacgattt
     5521 tgatactttt gatacagtta aaatgataac tgatatgatt cacgttgaga aaccttctga
     5581 agaaaaactg aagcaactgg gagtgcattc ctggccggta tggacaaagg aagaatcgga
     5641 attcccatgg gaatatgatg aaaaggagac ctgttatatt cttgaaggcg atgttactgt
     5701 tacacctgaa agtggagaac ctgtaagatt cgggaaggga gatcttgttg tttttcccga
     5761 gggtttgaaa tgcaggtgga aaattcacaa agctgtaaag aagcattaca ggttcggata
     5821 gcagaatgag aattgcggat attgcaatat ttttcatcta tcttgcggga ataattcttt
     5881 ttggcagttc tttttaccgt aagaacagga atgctactgc gtttacactt ggaaacagga
     5941 acatgcccac atggattgtg ggcatgtcta tttttgccac gtttgtaagc agtataagct
     6001 atctcgcatt accaggacag gcgtatcaga ccaattggaa tccatttgta ttcagtcttt
     6061 cactgccctt tgcagcatat atggcagtga agttcttcgt tccgttatac cggtcggtaa
     6121 acagcccatc ggcttataca tacctcgaga tgcgttttgg aaaatgggca cgcatatatg
     6181 cttcagtgat gtatcttctg actcaactaa tgcgtacggg tacaattctt tacctgctgg
     6241 ccatgatgct caacactgcc attggatggg atactgcgac cattatcata ataaccggaa
     6301 taagcataat gatttactca gttttgggag gcatacaggc agtgctctgg acagatgcaa
     6361 tacagggcat catacttatt gcaggcgcag ttgtgtgttc agtactattg ctgttttcaa
     6421 tgcctgaagg tccgggtcag ttgtttcaaa tcgcatccct gcacgataag ttcagcctgg
     6481 gtagttttaa acccgatttt accacttcaa ccttctgggt tgtatttatt tatggtatat
     6541 tcatcaacct tcagaattat ggtatagatc agaattatat ccagagatat cttacaacaa
     6601 ggaccgatag tgaagcaaga aaatcagccc tgttcggttc actgatttac atacctgtgt
     6661 cacttttgtt cgtctttatc gggactgccc ttttcagctt ttatacagca atgcctgagc
     6721 tacttgaggc gggtattgca ggcgacaggg tattccccta ttttatcttt caccagctcc
     6781 cggcaggtgc aactggattg cttatttcat ctattttcgc agctggcatg agtaccatat
     6841 caacaagcat caatagctcc gcaacagtaa tagtaactga ttacttcaga ctctctcccg
     6901 atgtaaaaga acacgaaaag aaatcaatga agattcttta catttcttct gttcttttca
     6961 gtttgtctgg cattctcgta ggaatagcta tgataaatgt tcagagcgcc ctcgatgcat
     7021 ggtggaaact tgcttcagtc ttcagcggtg gaatgctcgg tctgttcctg ctgggttttt
     7081 tttcaaaaaa agtaaacaat gcagccgcag tggcaggtgt cattaccgga attattgtaa
     7141 tagcatggat aagcctgtcg cctgtgcttt ttaaaggctc tctgcaaaaa tttgcatgcc
     7201 cattacacgg ttatctctca atcgttctgg ggacaactgc catattcata acgggtttca
     7261 ccatcggaat aataattgac agaacaagaa agaacagaaa atagttaatt gttactataa
     7321 atccagttct acaagggtga ttcctgcacc tcccctttct acatgttcat cggaaaatga
     7381 ttttaccaca tcaactgttg aaagatactg tcgaatgagc tgtcgaagta ttccgttgcc
     7441 tttaccgtga ataatcctga gatgcctgtg ctgaaccatt attgcatcat caatgaaatt
     7501 acgcactatg cttattgctt cttcgcccct cacacccctg atatctattt ctggtttgaa
     7561 actcattttt cgttgggata ttaccgggtc ggcctgcata acaacctgtc cctgccgcag
     7621 acttttctta agttctgccc tgctgatcct ttcgagtttt tctaatgaaa cctgaaacct
     7681 gaacgagcct gtctcaactg ttgccatttc atctcttatc tcaacaattt ctcctgcagc
     7741 catagtatct gccattctta cagcatcacc gacccttaaa ccttgctcct caggcttttc
     7801 tatcggtttt tcagtctttt ttacctttct tttaatctgc tttacttttt catcaattat
     7861 tgacaattgg gtgtcgctgg cagaagcaac ctctgaagca acctctttcc tgaatttttc
     7921 gagttcctcc cttgccattt ttgtcttttc cttt
//
LOCUS       DAOSNB010000092         7923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9124_length_7923_cov_3.994026, whole genome shotgun sequence.
ACCESSION   DAOSNB010000092 DAOSNB010000000
VERSION     DAOSNB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9124_length_7923_cov_3.994026"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLG42_05205"
     CDS             <1..556
                     /locus_tag="PLG42_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOU30642.1"
                     /translation="ATTVDRSNKENIELVNEKAKAGYTTGYADPEYIGSLPVFNLPFL
                     SENRKYRTFQLTGDSMLPIPDGSYVTGEFLQDWRNIITGNAYIILTLDDGIVFKVVEN
                     NIEKNGKLVLYSLNPLYEPYEVHVNEVKEVWKFVHYISSKLPDPLIPEKQLLKTVATI
                     KRDLERLKAKLGTEIIDITEEASQ"
     gene            complement(557..1093)
                     /locus_tag="PLG42_05210"
     CDS             complement(557..1093)
                     /locus_tag="PLG42_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HOU30643.1"
                     /translation="MKRKIIVSLSIIIASLSLKGQAVNLEVGVKAPNFVLTDAQKKEF
                     SLDSWPGKVLQINYVDPDEQDLNEPFNEAIDKAVDVDKRISRDHFKGFGIVDCKSTWK
                     PNSLIRVIAGNKAKKYDTTILFDYDATLQKLWGLPRDSYSIIILDKNRVCRAIYRGKV
                     PDNEIEKVIQLIIELTKE"
     gene            complement(1187..1855)
                     /locus_tag="PLG42_05215"
     CDS             complement(1187..1855)
                     /locus_tag="PLG42_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOU30644.1"
                     /translation="MVAIQKNTVEFLKALALNNNRDWFEAHRVNYETSRKNFEAFVQA
                     FIDRITEFDPVLKGLEAKSCIFRINRDIRFSNDKSPYKTNFGALIIRGGRKNIHRFAG
                     YYIHLEPGEYMIAGGAYLPPPEWLAAIREKIAEKPEEFLKIIENKDFKKYFGELDDEK
                     LKSAPRGFSPDHPYIELLKFKSYTVVNYISEKKVLSPDYLDYAVKVAEVLKPFNDFFN
                     ECAV"
     gene            2194..4305
                     /locus_tag="PLG42_05220"
     CDS             2194..4305
                     /locus_tag="PLG42_05220"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU30645.1"
                     /translation="MSNDIWKYFIGNVEPPSNWAATEFDDSSWLNGKGGIGYGDGDDS
                     TVISPAGSVYMRIKFNVYDADAVGAAILFVDFDDGFVAYLNDHEIARENIGTPGVRPS
                     HDEYAILTSYEAQMLSGGLPARFVINYDTLLKYLRTGENVLALQVHNCNSTSSDLSST
                     TFLIFGIKSEERLYRPAPSWFIEPKNDLTHLPIIVIDTKNQEIPDEPKIIVNLKVINN
                     GPGTMNSWLQPGTDFDGQASIEIHGQSTQMFPKKSYSIELKDYQGNDISVPLLGMPAE
                     EDWILHAHYTDKTMIRYALTLHLGSKMGSWQPRFRYCVVYLNGEYNGVYLLIEKIKRD
                     KERLDISRLRREDVAGDEVTGGYILKVDKIGGLTSNDYFVSYPYIIYPNSRNYKFTYV
                     YPKSDSIVYEQREYIKKYITDFQNVLNDKDFKDPFTGYKKYIDPSSFIDFQIIQELTN
                     NVDGYRFSTFFYKKKDTQGGKLYAGPLWDFDLCYGNVNYSDYNLSTSGWLYPHCGPTD
                     AYTMHWWKRLMEDPDYVRNFIMRWNELRKTYFSTENIMQFIDSIVLYLGPEIDRNYQK
                     WPILGKYVWPNYFIGKTYEEEINYLKQWINDRLTWMDAQTGLNSSLYNDAFDSEFIAY
                     PNPFDEKIKLIFSTRSTLPVSMEIYDFMGRMCLNDKIIPEYSGIQQIEKDFSELNNGV
                     YIIILKQSNRIIGKRIVIKRL"
     gene            complement(4761..5930)
                     /locus_tag="PLG42_05225"
     CDS             complement(4761..5930)
                     /locus_tag="PLG42_05225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU30646.1"
                     /translation="MKKLIAIFISISITYGALAQMGKVTSALSYIDQGLLDKAKEALD
                     QALVNEKSKNNPKTWVAKGRLAQECFRSDNPKFKAFYADPLEEALAAYEKALELDPKG
                     TIDKQLKLNNTYVLLGNDFITQGVQRFEAQDFAGALKSFENNIKVATSPLYIGVPDTG
                     IYFNAGLAAYNAKMYDRAIVHFQKCADMKYEGPMPYFLIYSCYKEMNDLANAEATLKK
                     AFELYPNNQDVILNLVDFYMTTDKLDEAFSYLNMAKSQKENDYTLYWAEGVLYMKQEK
                     YDEAIASLKKSIELKGDEYNTQFNLGVCYYNKAVAMFQKANEIMDVAKYNAAIAEANA
                     VFIQAVPYFEKAAELKKDDPDSLRNLKELYFRLRTVNPEFEAKYNEVVKKLEQIQ"
     gene            complement(5980..>7923)
                     /locus_tag="PLG42_05230"
     CDS             complement(5980..>7923)
                     /locus_tag="PLG42_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOU30647.1"
                     /translation="AFIENRNITVNELLQYVKGPDFPTGGIIYGQQGIKDAYETGRGK
                     IIIRARTEIEHTHSGRECIVVTEIPFMVNKAEMIKKIADLINDGKLEGISYINDESDR
                     NGTRVVIILKKDATANVVLNNLYKYSQLQTAFNVNNIALVKGRPRLLSLKDLIDLYVK
                     HRHDVILRRTKFDLDQAEKRLHILEGLIIASDNIDEVVAIIRGSSNPDEAREKLIERF
                     NLSEEQARAIVDMRLRQLTGLEQDKLRAEYEEVKKQIEYLRNVLGSIDLQMKIIKDEL
                     LEIKEKYGDDRRTEIVPNVEEFNPEDFYSDDEMVITISHLGYIKRTPLTEFRRQNRGG
                     IGSRASTIRDEDFIEHLYIATMHNTMLFFTGKGKCYWLKVYNIPEGTRTSKGRAIQNL
                     INIEPDDNIRAFINVKTLDDEKYIKNNYIVLCTAKGIIKKTSLEAYSRPRSSGVNAIT
                     VRDGDELIEARLTNGNHEIMLAVKSGRAIRFHESSVRPMGRTASGVRGIKLASDDDIV
                     IGMITIENKEKDVLVVSEKGNGKRTSIDSYRITNRGGKGVKTINITEKTGSLIALKDV
                     TDNDDLMIITLNGNILRIPVSGIRLMGRATQGVKLINLRENDSIASVACVEINEEDGG
                     EEADYDDENFSPSNENGKDFDEF"
BASE COUNT         2366 a         1491 c         1402 g         2664 t
ORIGIN      
        1 tgccacaact gtcgacagaa gcaataagga aaacattgaa ctggtaaatg aaaaagcgaa
       61 ggctggctat acaacaggtt atgcagatcc tgaatatata ggaagcctgc cggtgtttaa
      121 cctgcctttt ctttcagaaa acagaaaata ccggactttt cagcttacag gcgattcaat
      181 gttgcccata cccgacggct catatgttac cggcgaattc cttcaggact ggaggaatat
      241 cataaccggc aatgcatata ttattttaac tctcgatgat ggtatagttt ttaaagtagt
      301 ggaaaataac attgaaaaaa acggcaagct ggttctttat tcactgaacc ctctgtatga
      361 gccttatgaa gtgcatgtta atgaagtaaa ggaagtctgg aaatttgttc attatataag
      421 tagcaagctt cctgacccct taataccgga aaaacagctc ctgaaaacag tagctacaat
      481 aaaacgtgat ctcgagcgcc ttaaagcaaa gttgggaact gaaataattg atattactga
      541 agaagcaagc cagtaactac tcctttgtca gctcaattat cagctgtatt actttttcta
      601 tttcgttatc tggaacttta cccctgtaaa tagccctgca gactctgttc ttatccagga
      661 taattatgct gtaactatcg cggggaagtc cccataattt ctgaagtgta gcatcatagt
      721 cgaatagaat ggtagtatca tattttttgg ctttgttgcc tgctatcacc cttatcagac
      781 tgttgggttt ccaggttgat ttgcagtcga ctataccaaa acccttaaaa tgatccctgc
      841 ttattctttt atcaacatcc acagccttat caatggcttc attgaatggt tcattcagat
      901 cctgctcatc gggatccaca taatttatct gcagtacctt gccaggccat gaatcgagag
      961 aaaactcttt cttctgagcg tcggtaagaa caaaattggg ggcctttact ccaacttcaa
     1021 ggtttacagc ttgcccttta agacttaatg aagcaataat gatgcttaat gaaacaataa
     1081 ttttcctttt catatctgct gttttataat tttaacgaat atagaaattt tttataataa
     1141 ctttttactg attttcaaat ccataagttt gacatgaaat gatttgttat acagcacatt
     1201 cattgaaaaa atcattaaaa ggtttgagaa cttctgccac cttaactgca taatcaagat
     1261 aatcaggact cagcaccttc ttttcactta tataatttac aactgtatag cttttaaact
     1321 tcagtaattc gatataaggg tggtcaggac tgaagcccct gggagcactc ttaagttttt
     1381 catcatcaag ttctccgaaa tactttttaa aatctttatt ttcaatgatt tttaaaaatt
     1441 cctcaggttt ttcagctatt ttttccctta tagccgccag ccattcagga ggcggtaaat
     1501 aagcaccccc agcgatcata tattctcctg gttcaagatg aatataatat cctgcaaatc
     1561 tgtgaatgtt tttcctgcca cctctgataa tgagggctcc gaaatttgtc ttatatggag
     1621 atttatcgtt tgaaaatcgt atatcacggt taatcctgaa gatacaggat ttagcctcga
     1681 gtcctttcaa aaccggatca aattcagtta ttctgtcaat gaatgcctga acaaatgcct
     1741 caaaattctt cctggaagtt tcataattaa cacggtgtgc ttcaaaccaa tctcggttgt
     1801 tattcaatgc aagtgcttta agaaattcaa ccgtattttt ctggatagca accatattgt
     1861 tattgttctg gtttcgaaat atagaaaata atagataaaa acttactcat attttaaacc
     1921 ttcctgtatt tatctatttt ttaaaataaa ttgcttataa aaaagtttaa agattaagtc
     1981 aaagcagggt caatttaaat attcatggtg taatgtaaat aggatattga attataatga
     2041 ttaatttaat cactttgtaa tttttaaatg tagtgtttca ataagatatt gattaacaat
     2101 tttttaaaga tattatttat tgttgcagtt ctgacactta agtgttcagt ctgcaatttt
     2161 gcccaggaaa ttgatcactg ggaaactctt gttatgtcga atgacatatg gaaatatttc
     2221 ataggcaatg ttgaacctcc atccaactgg gccgctacag aatttgatga ttcatcgtgg
     2281 ttgaacggca aaggagggat tggatatggc gacggtgatg attctacggt tatttccccg
     2341 gcaggctctg tatatatgag gattaagttc aatgtttatg atgctgatgc tgtaggtgct
     2401 gcgattcttt ttgtagattt tgacgatggt tttgttgctt acctgaatga tcacgaaata
     2461 gcaagagaaa acattgggac accaggcgtg aggccatccc atgatgaata tgccatactg
     2521 acatcttatg aagcacagat gctctcggga ggtttgcctg caaggtttgt tattaattat
     2581 gatacattat taaaatatct cagaaccggc gagaatgtct tagcccttca ggtacataac
     2641 tgtaattcaa cctcttcaga tctatcttcg actacatttc tgattttcgg aattaaatca
     2701 gaggagaggc tttacaggcc tgcgccttcc tggtttatag aaccaaaaaa tgatcttacc
     2761 catcttccta ttattgtaat agatactaaa aaccaggaaa ttcctgatga acctaaaatt
     2821 attgtcaatc tgaaagttat taacaacgga ccgggtacga tgaattcgtg gcttcagccg
     2881 ggaactgact ttgatggtca ggccagtata gaaatccatg gacaatcgac acaaatgttt
     2941 cccaaaaaaa gttattcaat tgaacttaaa gattaccagg gtaatgatat tagtgtacct
     3001 cttctgggaa tgcctgcgga agaagactgg atacttcatg ctcattacac tgataagacg
     3061 atgataagat atgcacttac cctgcatctt ggaagtaaga tgggatcatg gcagcccaga
     3121 ttcagatatt gtgttgttta tttgaatgga gagtataatg gtgtttatct tttaattgaa
     3181 aaaataaaaa gggataagga acggcttgat atcagccgtc tgagaaggga agatgttgca
     3241 ggagatgaag tgacaggagg atatatttta aaagttgaca aaattggcgg gctgacatcc
     3301 aatgactatt ttgtatcata tccttatata atttatccta attcaagaaa ttacaagttt
     3361 acatacgttt atccaaaatc agattcgatt gtttatgagc aaagagaata tattaaaaag
     3421 tatattacag attttcagaa tgttttgaac gataaagatt ttaaagaccc ctttacaggt
     3481 tacaaaaagt acattgatcc ttcatctttc attgattttc agataataca ggaacttaca
     3541 aataatgtag acggttaccg cttcagtaca tttttttata aaaagaaaga cacacagggt
     3601 ggtaaacttt atgccgggcc tttatgggat tttgatttgt gctatggaaa tgtgaattat
     3661 tctgattata acctttcaac atcgggatgg ctttatcctc attgcgggcc gacggatgcg
     3721 tatacgatgc actggtggaa gcgattgatg gaagatcctg attatgtaag gaatttcata
     3781 atgaggtgga atgaacttag gaaaacatat ttttcaacag agaatataat gcaatttata
     3841 gacagtattg tattgtatct gggcccggaa atagaccgaa attatcagaa atggcctatt
     3901 ttgggcaaat atgtatggcc aaattatttt atcgggaaaa catatgaaga ggaaattaat
     3961 tacctgaaac agtggataaa tgacaggctt acctggatgg atgcacaaac tggcttaaac
     4021 tcaagtcttt ataatgatgc ttttgatagt gagtttattg cttacccaaa tccttttgat
     4081 gaaaagataa agttaatttt ctcaacacgg agcacattgc ctgtatcaat ggaaatttac
     4141 gattttatgg gtcgtatgtg cctgaatgac aaaataatac ctgaatattc aggaattcaa
     4201 caaatagaaa aggatttctc agaattaaat aatggagtat acattattat tctgaaacag
     4261 agtaacagaa taattggcaa aaggattgta attaagaggt tgtaattaat tggtgtgtca
     4321 atatctggat gccggttact ggttgtcaga ttcagaaata tcccaggtta aagttatgcg
     4381 gatgttaacc agcaaagctg gaagggaaaa aatcgtaccg ttccggtgcc agaggccccg
     4441 gaactgattt tatgttaacc agcaaagctg gacggaaaaa tataaaagag gtatgaagaa
     4501 atttccttta acataaaatc gtaccgttcc ggtgccagag gccccggaac tgattttatg
     4561 ttaaccagca aagctggacg gaaatttctt catgattccc ataattaata ttatgttaaa
     4621 tagtattttt attcataaag gaattctata ttaataattt aattctatat taataattta
     4681 attacctaac tctccccttt ttcgcaggaa taaaaaacgc ggttgagtca caaccgcgtt
     4741 ttaattattt atattcgttt ttactgaatc tgttcaagct tctttactac ctcgttgtat
     4801 tttgcttcaa attcaggatt aactgttcta agtctgaaat agagttcctt caaatttctc
     4861 agagagtctg gatcgtcttt cttgagctca gctgcttttt caaaataagg cacagcctgg
     4921 ataaatactg catttgcttc tgctattgca gcattatatt ttgcaacatc cataatttcg
     4981 tttgctttct gaaacattgc aacagcctta ttgtaataac agactccaag attaaactga
     5041 gtattatact catccccttt caattcaatg gattttttaa gacttgctat tgcctcatca
     5101 tatttttcct gcttcatgta taatactcct tcagcccaat agagtgtgta atcgttttct
     5161 ttttgacttt ttgccatgtt cagatatgag aaggcttcat caagcttatc agttgtcatg
     5221 tagaaatcaa ccaggttaag tataacatcc tgattgttag gatataattc gaatgctttt
     5281 ttcagggtag cctcagcatt tgcaaggtca ttcatttcct tgtagcagct gtagataagg
     5341 aaatatggca tcgggccttc atatttcata tcagcacatt tctgaaaatg tactattgcc
     5401 ctgtcataca tttttgcatt gtaagctgca agacctgcgt tgaaatagat gcccgtatca
     5461 ggaactccga tatatagtgg actggtagca actttaatat tattttcaaa tgatttcaga
     5521 gcacctgcaa aatcctgagc ttcaaatctt tgaactccct gagttataaa gtcgtttcca
     5581 agaagcacat atgtattatt aagcttgagc tgcttgtcga tagtaccctt gggatcaagt
     5641 tcaagggcct tttcataagc tgccagagct tcttcaagag gatcagcata aaacgcttta
     5701 aacttgggat tatctgatct gaaacattcc tgtgccagcc tacctttggc aacccaggtt
     5761 ttaggattat tttttgattt ttcattaacc agagcctggt caagagcttc ttttgcctta
     5821 tcgagcagtc cctgatcaat ataactcagc gcactggtta ctttccccat ctgggccaaa
     5881 gcaccgtaag taattgaaat cgaaataaat attgcaatta actttttcat ggtttaaatt
     5941 ttagttataa aaatcgcttc aaatttaaaa cacttttaat taaaattcat caaaatcttt
     6001 gccattttca tttgatggtg aaaaattttc atcatcataa tcagcttctt ccccaccatc
     6061 ctcttcatta atttccacac atgcaacaga tgctattgaa tcattttctc taagatttat
     6121 gagcttaaca ccctgtgttg cccttcccat cagtctgatt cccgatacag gtatcctgag
     6181 gatatttcca ttcaatgtta ttatcataag atcatcatta tcagtaacat ctttaagggc
     6241 gataagtgag ccggtttttt cagtgatatt tatggttttc acaccctttc ctcccctgtt
     6301 tgtaattcta taactatcta tgctagtacg ttttccattt cctttttccg aaacaaccag
     6361 aacatctttc tccttatttt cgattgtaat cataccaata acaatatcat catccgaagc
     6421 aagttttata cctcttactc ctgaagcggt tcttcccatt ggtctaacac tgctctcgtg
     6481 aaatctgatt gcccttcccg attttacagc aagcattatt tcatgatttc cattagtaag
     6541 gcgagcttca ataagttcat ctccatctct tacagttatg gcattaaccc ccgaacttct
     6601 tggtcgtgag taggcctcaa gtgacgtttt tttaataatt ccctttgcag tgcataaaac
     6661 aatgtaattg ttttttatgt atttctcgtc atcaagggtt ttaacattaa tgaatgctct
     6721 gatattgtca tcaggttcaa tatttattag attctgtata gctcttcctt tagaggttct
     6781 agtgccttcc gggatattat atactttaag ccagtaacat ttccccttgc ctgtgaagaa
     6841 gagcattgta ttatgcatgg ttgcaatata aagatgttca atgaaatctt catcgcgtat
     6901 tgtactggcc ctcgacccta tgcccccgcg attttgcctg cgaaattctg tgagaggggt
     6961 tcttttaata tatccgagat gggatattgt aataaccatc tcatcatcag aataaaagtc
     7021 ctcgggatta aattcttcaa catttggtac tatctccgta cgcctgtcgt cgccgtattt
     7081 ttcttttatt tcaagcagct catcctttat aattttcatt tgcagatcaa tacttcccag
     7141 tacattcctc agatactcaa tctgcttttt cacttcctca tattctgctc tgagtttatc
     7201 ctgttcgagg cctgtaagct gcctgagtct catatcgact atagccctcg cctgttcttc
     7261 cgaaagattg aatctctcaa taagtttttc cctggcctca tcaggatttg atgaacctct
     7321 tataattgca acaacctcat caatattgtc gcttgcgata ataagaccct ccagaatatg
     7381 caaacgtttt tcggcctggt caaggtcaaa ttttgttctt ctcagaataa catcatggcg
     7441 gtgttttaca tataaatcta taaggtcttt aaggctgagc aggcgcggcc taccttttac
     7501 aagggctata ttgttaacat taaaagctgt ctgtagctgt gagtacttgt agaggttgtt
     7561 tagaactaca ttggcagtag catctttttt aagaattatt acaacccttg ttccatttct
     7621 gtccgattca tcgttaatgt aggagattcc ctctaattta ccatcattaa tcaaatctgc
     7681 tatttttttg atcatctcgg ccttatttac cataaaggga atctctgtaa ccacaatgca
     7741 ttctcttccg gaatgggtat gttctatttc tgttcgtgcc cggataatga ttttacccct
     7801 tcctgtttcg tatgcatctt taatcccctg ttgaccgtaa attatccctc ctgttgggaa
     7861 gtcaggccct ttaacatact ggagtaactc gttaacagta atattcctgt tttctataaa
     7921 agc
//
LOCUS       DAOSNB010000093         7895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9166_length_7895_cov_4.647194, whole genome shotgun sequence.
ACCESSION   DAOSNB010000093 DAOSNB010000000
VERSION     DAOSNB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9166_length_7895_cov_4.647194"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLG42_05235"
     CDS             <1..373
                     /locus_tag="PLG42_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU30648.1"
                     /translation="QRLADFVEGKFSSSLPEVSYFPGVTSSAVHELLPPFIISRLQNG
                     FRYFGKVMKGFLTNEAVVMAVESRTSSPVRILRNENTLEHIRISGLYPCGEGAGYAGG
                     IVSSAVDGIRVAEAISSRIKP"
     gene            complement(381..1163)
                     /locus_tag="PLG42_05240"
     CDS             complement(381..1163)
                     /locus_tag="PLG42_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOU30649.1"
                     /translation="MLSKNKQKFIISLQKKKAREETGMFVIEGDKMVKEFMAAGAEIV
                     MLIAKPEYIAGLGKKQMIKDYEVIPVTFDELRKVSTMKTPHNALAVVKMPDKEFNADT
                     ILDDLSVAMDTIQDPGNLGTIIRAAAWFGIKNIVCSENSVDVYNPKVVQATMGALLNV
                     NVFYRNLDELLDYARKKGADVFGTYLDGCPIYEHPLGKKGIILLGNESKGISENLTPY
                     VTARLTIPAFKTLQYGVESLNAGMAASIVFYEFRRRKQEQSD"
     gene            1281..3575
                     /locus_tag="PLG42_05245"
     CDS             1281..3575
                     /locus_tag="PLG42_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOU30650.1"
                     /translation="MLLLIFVIACNPAKYIPSGETLLDRNYVKINNESIKKSEIYPYI
                     RQKPNKRIFGARFHLGLYNLSNIKKERWPHNWLRNIGEEPVIFDRSAVDKSVEQIRAY
                     LVSKGYFDSRVSETIETAKKQTKVYYDIILNRPYTIHNITYEVEDTTIAKIVYFDSTA
                     CLIERGKPYDVETLQAERSRQERFIRDHGFYNFSAEHIYFRVDSTIPGRQVDIIYGIR
                     NFMRIGHGNKPVLVPHMQYKLRNIYIYPDYNPRDFLAGAEEYQKRLDTIDYRGYFFVT
                     AQKRPLINYDLLIQSLYLRPGYTFNQTSTERTHARLMSLRTYRLVNILYNEVEEMPEG
                     GDYRYLDCIIQLTPVSRQSFSVELEGTNSGGNLGGALNFIYQNRNLFHGAEQFNLKLK
                     GAYETFSQTTTGIKSTQEFGVETSLRLPKFFLPFLESENFIRKYTPSTTIQAAYNYQN
                     LPVYTRTIANASFGYQWNQDAYRTHIVYPVQMNFVKIPSIEEEYYRNVIEKSSYLINS
                     YKDVLIAGGSYSYIFSRQNIRRSRDNWFMRINVESSGNILRTAMKLAGVKPVTDSVYS
                     HYNIFGQPFAQFVKGDIDIRYTRIINDISSIVFRGFAGAGIPFKNSLAMPFEKQYFEG
                     GANGVRGWQVRSLGPGSYSPPESKYINQTGDIKLEANVEYRFKLFWILEGAVFVDAGN
                     IWAIREDPDRPGAKFNLNKFYDDIAIGTGFGMRFDLKFVLLRADLGFKFRDPKYTGSD
                     RWLLTRQSGTGNNMALVIAIGYPF"
     gene            3678..4346
                     /locus_tag="PLG42_05250"
     CDS             3678..4346
                     /locus_tag="PLG42_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOU30651.1"
                     /translation="MRFIRDFFGMSRSERRATLLLSVLIFLVIIVRFAAPARKVPLEY
                     FTLSVNSLLPADSSDSARREDQSIEPGQPGKNFKTSQGVGSGKAMIDLNLADSVELVR
                     LPGIGPVLASRIIKYRNFLGGFVSTSQLGEVYGITDSLCRKLLPVLTADTSAVRKIFI
                     NIPGFDRSLRHPYISKDEADAITELRGSGYIFKSVNDLVDKNIFSREKARKLKYYIDF
                     SEGF"
     gene            4562..4753
                     /gene="rpsU"
                     /locus_tag="PLG42_05255"
     CDS             4562..4753
                     /gene="rpsU"
                     /locus_tag="PLG42_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOU30652.1"
                     /translation="MIIVPIKDGKNIDRALKKFKRKFEKTGIIRELRARQAYVKPSDR
                     RREQIKRAIYIQKLQQREE"
     gene            4797..5684
                     /locus_tag="PLG42_05260"
     CDS             4797..5684
                     /locus_tag="PLG42_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOU30653.1"
                     /translation="MNYKESFLQYLKSEKRYSPHTLSSYENDLDKFLEFLGSVGHSGV
                     LSEVTSQDVRAWIVDMMDKGFTAVSVHRKISCLRGFYRYMVKQGMVDRNPLEKVVLPK
                     RKKSLPVFVDEEALKTLLDSFDFGDDFKGIRNRNIIEMLYTTGMRRAELTGLRCDDVD
                     LMNNTVKVTGKRNKQRIIPILPSFSERLKEYLKKREEEFSSGCEEWLFLTDSGKRMYD
                     KIVYNIVKSYLSLVTTIEKKSPHVLRHTFATHMLNRGADLNSIKEFLGHANLSATQVY
                     THNTFEKLKEIYKRAHPRA"
     gene            5702..6001
                     /gene="raiA"
                     /locus_tag="PLG42_05265"
     CDS             5702..6001
                     /gene="raiA"
                     /locus_tag="PLG42_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOU30654.1"
                     /translation="MNIKIHSVRFDADKRLIQFVNQKIEKLNHFSEGIVSAEVYLRLD
                     NDQEKENKIAEIKIEYPGGPLFAKKQSKTFEEATDLAVDAIKKQIVKQKEKMKGI"
     gene            6116..6189
                     /locus_tag="PLG42_05270"
     tRNA            6116..6189
                     /locus_tag="PLG42_05270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6150..6152,aa:Thr,seq:tgt)
     gene            6243..6325
                     /locus_tag="PLG42_05275"
     tRNA            6243..6325
                     /locus_tag="PLG42_05275"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6277..6279,aa:Tyr,seq:gta)
     gene            6381..6453
                     /locus_tag="PLG42_05280"
     tRNA            6381..6453
                     /locus_tag="PLG42_05280"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6414..6416,aa:Gly,seq:tcc)
     gene            6465..6536
                     /locus_tag="PLG42_05285"
     tRNA            6465..6536
                     /locus_tag="PLG42_05285"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6497..6499,aa:Thr,seq:ggt)
     gene            6601..7785
                     /gene="tuf"
                     /locus_tag="PLG42_05290"
     CDS             6601..7785
                     /gene="tuf"
                     /locus_tag="PLG42_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOU30655.1"
                     /translation="MAKEKFERTKPHVNIGTIGHIDHGKTTLTSAITRVLNKKGLAEV
                     KTFDQIDNAPEETERGITINTAHVEYQTEKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAADDGPMPQTREHILLAHQVNVPAIVVFLNKVDLVQDEELLDLVEMEVRELLNF
                     YKYDGDHCPVIRGSALGAMNGEPKWEEKVLELMNAVDEYIPIPQRDIDKPFLLPVEDI
                     FSITGRGTVATGRIETGVVQTGDELEIVGLGAKKKTVCTGVEMFRKILDRGEAGDNVG
                     LLLRGIDKKEIKRGMVLAKPGSITPHTKFKAEVYVLKKEEGGRHTPFHNKYRPQFYIR
                     TLDVTGEIILPEGREMVMPGDNVNLTVELIYPVAINVGLRFAIREGGRTVGAGTITEI
                     IE"
BASE COUNT         2383 a         1451 c         1799 g         2262 t
ORIGIN      
        1 acagagactt gcagattttg tcgaggggaa gttctcttcg tcgttgccgg aagtgtcgta
       61 ttttccagga gttacttcat cagctgtaca cgagttactg ccacccttca taatcagcag
      121 gcttcagaat ggtttcagat atttcggaaa agtaatgaaa ggtttcctta ccaacgaagc
      181 tgttgttatg gcagttgagt cgagaacttc ttcaccggtc agaatcctga gaaatgaaaa
      241 tactcttgaa catatcagaa tttcaggtct ttatccctgt ggtgaaggag caggctatgc
      301 tggaggtatt gtatcatcag ccgtcgatgg tataagagtt gccgaagcca tttcatccag
      361 gataaaacca tgatgacaga ttaatcagat tgctcttgtt ttcttcttct gaattcgtaa
      421 aagactattg atgctgccat tcctgcattt agcgattcta caccgtattg taaagttttg
      481 aaagccggaa tggtaagtct tgcagttaca taaggggtaa ggttttcaga tatgcccttc
      541 gattcgtttc ccagaagtat aattcctttt ttccccagag gatgctcata aatagggcaa
      601 ccatcaaggt aggtgccgaa aacgtctgca cctttcttcc gggcataatc cagaagttca
      661 tcaagattcc tgtaaaacac atttacattc agcagggcgc ccatagtggc ctgaacaact
      721 ttcggattgt aaacgtccac actattctca gagcaaacaa tgttctttat cccgaaccag
      781 gccgcagccc gtataatggt acccagattt ccaggatcct gtattgtgtc catagcaaca
      841 gaaaggtcat caagtatggt atcggcatta aattctttat cgggcatctt tactactgcc
      901 agagcattgt gaggagtctt catggtactg acctttctca gttcatcaaa agtaaccggg
      961 attacttcgt aatctttaat catttgcttt ttacccagac ctgcgatata ttcaggtttt
     1021 gcaatcagca tgacaatttc ggctccggca gccatgaact ccttcaccat cttgtcaccc
     1081 tcaataacaa acatccctgt ttcctccctt gcctttttct tctgaagaga tatgataaac
     1141 ttctgtttgt ttttactgag catttaacgc tttattttga agtattatta gatatatttg
     1201 taaaatcact atctgaccga atcttgaagt atataatttc cgataaaagt aagaatttta
     1261 ttcaacccgg aatagcgtgg gtgttacttt taatttttgt aattgcctgc aatcctgcaa
     1321 aatatattcc ttcaggagaa acactactag ataggaatta tgtaaaaatc aataacgaaa
     1381 gtataaagaa aagtgaaata tatccatata tacgccagaa accaaataag agaatatttg
     1441 gcgcccgttt tcatcttggg ttatataatc tttctaatat aaaaaaagaa agatggcctc
     1501 ataactggtt gaggaatatt ggagaagagc cagttatatt tgacaggagc gctgttgata
     1561 agtcggtgga gcaaataagg gcatatcttg tatcaaaagg ctatttcgac agcagggttt
     1621 cagaaacaat cgaaaccgca aaaaaacaaa ccaaagttta ttacgacata attcttaaca
     1681 gaccatatac tatacataac attacctacg aagtagagga tacaacaatt gctaagatag
     1741 tctactttga ttcgactgcg tgtttaattg agcggggaaa accttatgat gtcgagactc
     1801 ttcaggctga aaggtcgaga caggagcgtt ttatacgtga tcacggtttc tataattttt
     1861 cagctgagca tatttatttc cgggttgaca gtacaattcc aggaaggcag gtagatataa
     1921 tatatggaat aagaaacttt atgaggatag ggcacggtaa caaacctgtt cttgttcctc
     1981 atatgcagta caagctgagg aacatatata tctatcctga ttacaacccg cgtgattttc
     2041 ttgcaggcgc tgaagagtac cagaaaaggc tcgacactat tgattacagg ggatactttt
     2101 ttgttaccgc ccagaagaga cctttaataa actatgatct gctgattcag tcactttatc
     2161 ttcgtccggg ttacactttc aaccaaacca gcactgaaag gacacatgca aggctgatgt
     2221 cacttagaac ttacaggctg gtaaatattc tttataatga agtggaggaa atgcccgaag
     2281 gaggtgacta caggtatctt gactgtatta ttcagctgac tccggtaagc aggcagtcat
     2341 tttcggtaga gcttgaaggt acaaactctg gagggaacct tggaggggca cttaatttta
     2401 tttatcagaa cagaaacctg ttccatggtg ctgagcagtt taaccttaaa ctcaaagggg
     2461 cttatgagac tttctcccag actaccaccg ggataaagag cacccaggag tttggcgttg
     2521 aaacaagtct gcggctccct aaattctttc tgcctttcct tgaaagcgaa aatttcatca
     2581 gaaaatatac tccttcaacc acaatacagg cagcatataa ctatcagaat cttcctgtct
     2641 acacacgaac cattgcaaat gcctcattcg ggtaccagtg gaaccaggat gcctaccgga
     2701 cccatatagt atatccggtg cagatgaact ttgtcaaaat tccttctatt gaggaagagt
     2761 attacagaaa tgtgatagaa aagtcgtcgt acctcataaa cagctacaag gacgtactta
     2821 tagccggtgg cagttattcg tatattttca gcagacagaa tataagaagg tcgagggaca
     2881 actggtttat gagaatcaat gttgaatcct ctggaaatat tctgcgtacc gccatgaaac
     2941 ttgctggtgt aaaacctgtt actgactcag tatattctca ttacaacata ttcggacaac
     3001 cttttgcaca gtttgtgaaa ggtgatatag atataaggta taccagaatt ataaacgaca
     3061 taagttcaat tgtctttagg ggctttgctg gtgccggcat tccgtttaaa aattctcttg
     3121 ctatgccttt tgaaaagcaa tattttgaag gaggtgccaa tggcgtcaga ggatggcagg
     3181 taagatcgct gggtccgggt tcttattctc ctcctgagtc gaagtacatt aaccagacag
     3241 gggatataaa gctcgaagca aatgttgaat accgctttaa gcttttctgg attctcgaag
     3301 gggcggtatt tgttgatgcc ggcaatatat gggctatcag ggaagaccct gaccgcccag
     3361 gggcgaagtt taacttaaac aaattttacg atgacattgc tatcggcacc ggcttcggaa
     3421 tgcgcttcga tctgaaattt gtgcttttaa gagcagatct ggggtttaaa ttccgtgatc
     3481 cgaaatacac gggaagcgac aggtggctgt taacccgcca gtcgggtaca ggcaataata
     3541 tggcacttgt gatagctata ggctatcctt tctgaatttt tttgttgaat ctgttaagga
     3601 tttttctttt aaagctgtaa tatctccgga atttattata ttgcattcag tatgacgctg
     3661 catttctgtt ttagaaaatg aggtttataa gagacttttt tggtatgagt cgcagtgaga
     3721 gaagagccac tctgcttctt tccgttctta tatttcttgt aataattgtc aggtttgctg
     3781 ctcctgcgag aaaggttcct cttgaatatt tcactctttc cgtcaatagt ttgctgccag
     3841 cagattcttc tgattcggca cgtcgggagg atcagtctat agagcccggt cagccaggga
     3901 aaaattttaa aacctctcag ggagtgggct ccgggaaagc aatgatagac cttaatctcg
     3961 ccgattctgt ggaactggtg aggttgccgg gaataggtcc ggtgcttgct tcgagaataa
     4021 ttaaataccg aaactttctg ggagggtttg tgtcaaccag ccagctcggt gaggtttatg
     4081 ggataaccga ctcactttgc aggaagcttc ttccggtgtt gacggcagat acttcagcag
     4141 tcagaaagat attcataaat atacctggtt ttgacagatc tcttcggcat ccttatattt
     4201 caaaagatga ggcagatgca ataactgagc taaggggaag cggatatatt ttcaaatcgg
     4261 ttaatgatct ggtagataaa aatattttca gcagagaaaa agcaaggaag ttaaagtatt
     4321 acattgattt ttcagaagga ttttagttgt atgcctctgg taatgaaatg ttttcaggac
     4381 tttttaatat tattttgtaa aaggttgggt ttttcgggaa aatttattat atttaatcag
     4441 ataacagagt tgaacgggca tgacaagcaa tatttacatg ggatcaaata aaaatggata
     4501 aaatattttg aaaaaagtta atttttaatt atttttgcgg ctcaattcaa aaggagtaat
     4561 tatgattatt gtaccgataa aagatggtaa aaacattgac agagctttaa agaagtttaa
     4621 gagaaaattt gaaaagacgg ggattattcg tgagttgaga gcccgtcagg cttatgttaa
     4681 accttcagat cgtcgcaggg agcaaattaa gagggctatt tacattcaga agctacagca
     4741 gcgcgaggag tagaattttc gctgcacatc gattaaaaat tcattctggt tccttcatga
     4801 attacaagga atcattttta caatatctta agtctgagaa gcgatattct cctcacacac
     4861 tatcgtcgta cgaaaatgat cttgacaagt ttctggagtt tcttgggtcg gttggtcact
     4921 caggtgttct gtctgaagtg acttcgcagg atgtgcgtgc gtggatagtg gacatgatgg
     4981 ataaaggttt tacggcggta tcggtacaca ggaagatttc atgcctcagg gggttttacc
     5041 ggtacatggt gaagcagggg atggttgaca ggaacccact tgaaaaggtg gttttgccga
     5101 agcgaaaaaa gtcgttgcct gtttttgtgg atgaggaagc attaaagacg ttgcttgaca
     5161 gttttgattt tggtgatgat ttcaagggta tcaggaaccg taatataatt gagatgttgt
     5221 atactaccgg gatgagaagg gctgaactga cgggactcag gtgtgatgat gttgatttga
     5281 tgaataatac agtaaaggtt acaggcaaaa ggaataaaca gaggataatt cccattctgc
     5341 cgtcgttttc tgaaaggctt aaggaatatc tgaagaagag ggaagaagaa ttcagcagcg
     5401 ggtgtgaaga atggcttttc ctgactgact ccggtaaaag gatgtatgat aagattgttt
     5461 ataatattgt aaagtcatac ctgtcgctgg tgacgactat tgagaagaag agtccgcatg
     5521 tattgcgtca tacatttgca acgcatatgt tgaaccgggg ggcggatctt aattctatta
     5581 aggagttcct tggtcatgcg aatctctcag ccacacaggt ttatacacat aatacatttg
     5641 agaaattaaa ggaaatttat aaacgtgctc atccaagggc ttaaaaagca aggaggtatt
     5701 tatgaatatc aagattcatt cagtcaggtt tgatgctgac aagaggctca ttcagtttgt
     5761 aaatcagaaa atagagaagc ttaatcattt cagtgaaggt attgtaagtg ctgaggtata
     5821 tttacggctt gataatgacc aggagaagga gaacaagata gctgaaataa agattgaata
     5881 tccgggaggg ccgttgtttg caaagaagca gagcaagaca tttgaagagg caactgatct
     5941 ggcggttgat gccattaaaa agcagatagt caagcagaaa gaaaagatga aagggattta
     6001 gtttataaaa ctataaaaat attttcaata attttgttaa ataacaatta aatttcatac
     6061 atttgcaaac cgattttcag ggggtaaaac caacctgaaa gaagttcttt aaaatgccga
     6121 tgtagctcag ttggtcagag cagctgattt gtaatctgca ggtcgggggt tcgaatccct
     6181 tcatcggctc ctgataggtg gatacagtga gacatacaga ggtaaagact ctgttatcag
     6241 caggggagat accaaagcgg tcaactgggg cagactgtaa atctgctggc tattgccttc
     6301 gtaggttcga atcctgctct ccccacacga tacagagaat cagctgtaca ggaaacggct
     6361 gagccttatc aggaaaaatt gcgggagtag ctcagttggc agagcatcag ccttccaagc
     6421 tgagggtcgc ggattcgaat tccgtctccc gctctatttt tgaggccaga gtagctcagg
     6481 ggtagagcac ttccttggta aggaaggggt caggggttca attcccctct ccggctcaat
     6541 agaaaacaag agtaataaca gatataaaat tcatattaac tttaacttaa agcttaaatt
     6601 atggctaagg aaaaatttga aaggacaaaa ccgcacgtaa atattggtac tattggccac
     6661 atcgaccatg gtaaaacaac tcttacttcg gccattacaa gggtgctcaa caagaaaggg
     6721 cttgcagaag taaagacttt cgatcagatt gataatgctc cggaagaaac agaaaggggt
     6781 ataacaatca atactgctca cgttgagtat cagaccgaga agaggcacta tgcacacgtc
     6841 gactgtcccg gtcatgcaga ctatattaag aacatgatta ccggtgctgc acagatggac
     6901 ggtgcaatac ttgttgttgc agctgatgat ggtccaatgc cacagacacg tgagcatatc
     6961 cttctcgcac atcaggttaa tgtgcccgct atagtagttt tcctcaataa ggttgacctt
     7021 gttcaggatg aagagcttct cgacctcgtt gaaatggaag tacgtgaact tcttaatttc
     7081 tacaaatacg acggcgacca ttgccctgtt atcagaggtt cggcactcgg agccatgaac
     7141 ggtgaaccga agtgggaaga aaaagtactt gaacttatga atgccgttga tgaatatata
     7201 cctattcctc agcgcgacat tgacaaaccg tttctgcttc cggttgagga tatcttctca
     7261 attaccggac gtggtacagt tgcaacaggt cgtattgaga caggtgtggt tcagacaggc
     7321 gatgaactcg aaattgtagg ccttggtgca aagaagaaaa ctgtatgtac aggtgttgaa
     7381 atgttcagaa agatcctcga tagaggtgaa gctggtgata acgttggtct gcttctcaga
     7441 ggtatcgata agaaggaaat caaaagaggt atggttctgg ctaaacctgg ttcgataact
     7501 ccgcatacca aatttaaggc agaggtttac gttctgaaga aagaagaggg tggacgccat
     7561 actccgttcc ataataaata ccgtccacag ttctatatca gaactcttga cgttaccggt
     7621 gaaataatcc tgccggaagg aagggaaatg gttatgccgg gtgataatgt caaccttaca
     7681 gttgaactca tttatcccgt tgctattaac gttggattac gttttgcaat tcgcgaagga
     7741 ggcagaacag ttggtgctgg taccataact gaaataattg aataaaacag gatatttcat
     7801 gatgtatcac cttctggcga tacatcatga aaatatctga aacacgggtg tagctcagtt
     7861 ggtagagcac tggtctccaa aaccaggtgt cggga
//
LOCUS       DAOSNB010000094         7892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9175_length_7892_cov_4.087916, whole genome shotgun sequence.
ACCESSION   DAOSNB010000094 DAOSNB010000000
VERSION     DAOSNB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9175_length_7892_cov_4.087916"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1201)
                     /locus_tag="PLG42_05295"
     CDS             complement(209..1201)
                     /locus_tag="PLG42_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1735 domain-containing protein"
                     /protein_id="HOU30656.1"
                     /translation="MKTLYNIIKLSAGILLVSSLMVSCIPEPESMDKGQTLIKLTSPT
                     EDGYLMYAIDGASYSQTVKVLDIRRDFANTEALNSSTTVVLEFDKADTSMLNKYNDTY
                     GTEFVPFPEGTYSINPPISSGNITLEFKPGEFAKEIYVTIPDATTLMEYAYAFAIKVT
                     VSGEGKWSEQSNDTLAIQIMPKNKYDGIYEVKAVSPMIDVVNSALTGLYPFKYVLETS
                     GLHSCRCFDSEIWFDYMHPIMSGTDTSGYGYFGLEVFFDPSGDGTIIDLKNPWGNPPG
                     NTRMPVLDPSGVNKWDPATHDIRIKYWMIQPSMVPDPPHIRTYFDELWIYKGPR"
     gene            complement(1238..2716)
                     /locus_tag="PLG42_05300"
     CDS             complement(1238..2716)
                     /locus_tag="PLG42_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOU30657.1"
                     /translation="MKRTIYKIISLALTVVIVAGCSKLEDFGDTNVNPAATTKPNTAA
                     LLTNVLSQIGGYAFIVRGGLYCQYFSETQYTDASLYADNSTDPSGAYSGILYDLENII
                     ITNTDEKTKDEAALNGANENQIAIARILKAYIFWTITDRWGDVPYSEALKGNPIVAFD
                     TQETIYKSLINELKEAVAQFTTSGGPIKGDIAYNGDIAKWKKLANSLRMLMALRLSKV
                     YPGANEYAATEFNAALNDPAGHIETNADNFALTYPGGNFKHPVYNLYDGRKDYGESNT
                     MTNIMTTVGGGDPRQAVYGATVTGAPSTNGVPYGWTRDKTEAWTAANPDWCYILHIDY
                     RQPTSTIYIVDASHVLLARAEAADRGWTAETANTKTLYEAGITASFTKWGLPAPTPGY
                     LSHSTVALANPFGTGANLQNIHMQQYIAYYPDGLQGWSHWRRTNVPALTPAEDAVNDP
                     PTIPRRYRYHQTNYALNGPALQEAISRLTGGDKMESRIWWDK"
     gene            complement(2749..5979)
                     /locus_tag="PLG42_05305"
     CDS             complement(2749..5979)
                     /locus_tag="PLG42_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU30658.1"
                     /translation="MKKLSLLIGLLAISVLTLFPQQTRVITGTVTSAVEGEGPLAGVT
                     VLVKGTTIGTATDVNGKYTLNVPVDAKTLVFSFIGMKTLEVEIAGRSVVDVVMESETV
                     GLAEIVVTSGYGIRRAPKSSSSLTQVVSGDRLNEVRPLNVNNALAGKVSGIQFQGQSA
                     AKLGAAGNIRLRGGSGFGEGSGVLYVVDGTILPNANDLNLDDIEDISVLSGPSASAIL
                     GDQGANGAIIITTKKARVSAGKTMGIEANLGFLASSVYVLPNYQNDYAGGAYYDLQKY
                     EWKPGHPEEWKALDGKYYHNYSDDASWGPRMAGQEYIPWYAWYGGHKYSYKTAKLLPQ
                     PNNARDFYETGFTYNNSVAFSKVSEGFNIRATLGNLYVDGLIPTTYLNRTNFSVKTSY
                     DLTKRLTFAANVNFFTTFTNGEFDDGYSNQTTGSFNQWFHRDLDMKIMKELKDVRTPD
                     GIWASWNHNDPTVYDPANPRNFYAANYWYNFYKYFDLVTNQERRDRLFGDVSLSYKII
                     EGLNFKVTYRRQQNNRWYEEKYSSDLNYSGTQTTGNEPRYKGYYRTETSYSNRENYET
                     LLSFSRKFGDFNVNANAGTDFFNSVLKSNRANTVDGLNVANLYTIANSKSQPNIVNDR
                     QNYKYRAAFLRGDVGFRDFLFGEFTLRNDWYSTLPPNNNSILSKSFGGSFVFSDLLQI
                     PFLNFGKIRASWGEIPRSIGVYEYPGFEYGVGQYQWTGNFLMGTPDRLVDPNIHGAVN
                     TQKELGLELRFLKSRIGLTATYWDGSERDIPVSVTISSFSGFTTKLVNTGEMIKKGID
                     LSLNFKPFDTPKFSWEINTTWAYLIKQEVTKIAEGIDRITVQNQGWDTPYLVHAINKP
                     WGEIFGSGMKVDEKGRPYLTKSGSYVKEAEKYYGNVLPKYTGGIQNSFKILKDISVTA
                     NFDYQVGGKFFSLSDMWGCFSGLMARTSGLNDKGIPIRDPVADGGGVRVDGVDVDTGE
                     PVTYYVDAQEYFHNLWNNDIYDSFIYDLTYFKFREFSIGYNVPLNKIGLSRYIQSATI
                     SLVAQNFWLIYSSTKDFDPSEVARAGGESGQFPGIRSIGTNIRITF"
     gene            complement(6115..6693)
                     /locus_tag="PLG42_05310"
     CDS             complement(6115..6693)
                     /locus_tag="PLG42_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOU30659.1"
                     /translation="MLRIGEYIFSLDLLQKKFACDLNACLGNCCRYGDAGAPLQENEV
                     DILENIIDKVSPYLRDEGRIAISEQGTSVQDFEGEMVTPLINGQECAYTILEENILKC
                     AIEKAWSEGKIDFRKPLSCHLFPVRIKKYEGFTAVNVEEWPICFPGREKGRKEGIYVF
                     EFLKDALKRAFGEDIYMQISMASELMRKKGNW"
     gene            complement(6713..>7892)
                     /gene="gpmI"
                     /locus_tag="PLG42_05315"
     CDS             complement(6713..>7892)
                     /gene="gpmI"
                     /locus_tag="PLG42_05315"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOU30660.1"
                     /translation="LLSDGGVHSLDKHLYYLCDMTMDYGLKNVFIHGFGDGRDTDPRS
                     GLGYMRNLLEHLKKSNGRVASFIGRYYAMDRDKRWERIKEAYDLIVYGKGKKTTDILA
                     AIQESYDNDITDEFMNPIVVTDENGNPVGTLKEGDVVIFFNFRNDRARELTIVLTQKD
                     MPEYGMHTIPLYYCTMTPYDATFKGLHVIYPKENADNTIGEVLGRTGKKQLRIAETEK
                     YAHVTFFFSGGREEVFPGEDRILIPSPKVPTYDLKPEMSAYEVKDAVIAEINKGKYDF
                     ICLNFANGDMVGHTGVYDAIWKAVVAVDDCVGQVVDAARKNGYDVLIIADHGNADKAI
                     NEDGTPNTAHSLNPVPVLLVSDDYKKVKEGILADVAPTILTIMGIPIPQEMTGKILVE
                     "
BASE COUNT         2097 a         1776 c         1536 g         2483 t
ORIGIN      
        1 acttgcaggg atggttctct attaccatgg taacataccg aaaagcaatg atattgttaa
       61 gattaataac attgtaatta aagtactcag ggcaacaccc acacgaattg atctgttaaa
      121 tctgaaagtg gaacaggatt agtgagtgtt ttatcttaga aaaaatgaaa aaccccgcct
      181 aagcggggtt ttttctattt taaagttatt atctcgggcc cttataaatc cagagttcat
      241 cgaagtaagt acggatatga ggaggatccg gtaccatgct tggctgtatc atccagtatt
      301 ttattcgaat gtcgtgtgta gctggatccc atttattaac ccctgatggg tcgagtaccg
      361 gcattctggt attacctggc gggttgcccc agggattttt cagatcaata atagttccgt
      421 cgcctgaggg atcaaagaat acttcaagtc caaagtaacc atagccactg gtatctgtac
      481 cactcataat cgggtgcata tagtcgaacc atatctcact gtcgaaacag cggcatgaat
      541 gtaaacctga tgtttccagc acatatttaa acggatataa gccagtgagt gcactattaa
      601 caacgtctat catggggctc actgccttta cttcataaat accatcatat ttatttttag
      661 gcataatttg tatagccagt gtgtcattag attgttcact ccattttcct tctccactta
      721 ctgttacttt aatagcaaat gcatatgcat actccatgag ggtcgttgcg tcaggaattg
      781 tcacataaat ctctttagca aattcacccg gcttaaattc taatgttatg ttacctgagc
      841 tgataggtgg atttatcgaa tatgtacctt ccgggaaagg aacaaattca gtaccataag
      901 tatcattata cttattcagc atcgatgtat ctgctttatc gaattcaagc acaactgttg
      961 ttgaactgtt tagtgcttct gtatttgcaa aatctcttct gatatccaac actttaactg
     1021 tttgtgaata acttgcacca tctatggcat acattaaata accatcttca gtgggcgatg
     1081 ttagttttat tagggtttgc cccttgtcca tcgattcagg ttccggaata cacgaaacca
     1141 taaggctcga taccagcaga atacctgctg ataacttaat aatattatat aatgttttca
     1201 tacttctaat aatttattct cattaagcat aattttacta cttatcccac catattcttg
     1261 attccatctt atcgccgcca gtaagtctgc ttatagcttc ctgaagggct ggaccattca
     1321 gagcataatt agtctgatga tatctgtacc ttctcggaat ggttggagga tcgttaacag
     1381 cgtcttcagc cggagtaagg gccggcacat tggtccttct ccagtgtgac catccctgaa
     1441 gtccgtcggg ataataagca atatattgct gcatatggat attttggaga tttgcacctg
     1501 taccgaaggg attagccagt gcgactgtgc tgtgtgacag gtatccaggt gttggtgccg
     1561 gaagtcccca ctttgtaaat gatgctgtaa tacctgcttc gtacagagtt ttggtgttgg
     1621 ctgtttctgc agtccatcct ctgtcggcag cttcggctct tgcaagaagc acatgtgaag
     1681 catcaactat gtaaatagtg ctggtaggct gacgataatc aatatgcagg atatagcacc
     1741 agtcggggtt agctgcagtc caggcttcag ttttatcacg tgtccatccg taaggaactc
     1801 catttgttga aggagcgcct gtgactgttg ctccatatac tgcctgacgt ggatcaccgc
     1861 ccccaactgt cgtcattata ttagtcatag tattgctttc gccatagtct ttacgtccat
     1921 catacaggtt gtacacagga tgcttaaagt tgccgcccgg ataggtaagt gcaaaattat
     1981 cggcattggt ttctatatgc ccggcaggat cattgagagc agcattgaac tcagttgcag
     2041 catattcatt tgcacccgga tagactttgg aaagcctcaa agccatcagc atccttaagg
     2101 agtttgccag ttttttccat ttggctatat caccattata agcgatatct ccttttattg
     2161 gtccgccgct tgttgtaaac tgagcaactg cttccttaag ttcgtttata agacttttat
     2221 agatggtctc ctgagtgtca aatgctacta taggattacc tttaagtgct tcgctgtaag
     2281 gtacatctcc ccacctgtcg gtaattgtcc agaaaatata agctttaaga attcttgcta
     2341 tagcaatctg gttttcgttg gcaccattaa gtgcagcctc atccttggtc ttttcgtcgg
     2401 tatttgttat aataatattc tccagatcat aaagaatccc ggaataggca cctgaaggat
     2461 cggttgaatt gtcagcataa agagatgcat cagtgtactg agtttctgag aagtactgac
     2521 agtatagacc tcctcgtaca atgaatgcat atcctccaat ctgtgagagt acatttgtta
     2581 aaagcgctgc ggtattgggc ttggttgtcg cagccggatt aacgttggtg tctccaaaat
     2641 cctcgagctt tgaacaaccg gcaacaatta ctaccgtcag tgccagactt atgattttat
     2701 atattgtcct tttcatcttc ttaaagttta ttgttttgaa aacggtatct agaaagtaat
     2761 tctgatattt gttccgattg aacgaatacc cgggaactgt cccgattcgc cgccggctct
     2821 tgcaacctct gaaggatcaa aatcttttgt gcttgaatat atcagccaga agttctgggc
     2881 aaccagagaa attgttgcgc tctggatgta tctgctcaat cctattttgt tcagaggtac
     2941 gttgtaacct atgctgaatt cgcggaattt gaagtatgta agatcatata tgaatgaatc
     3001 atatatgtcg ttattccaca ggttatggaa atattcctgt gcatccacat agtaagtaac
     3061 aggttcgcct gtatccacat caactccgtc aacacgcaca ccacctccgt cagcgaccgg
     3121 atcacgtatc ggaatgccct tgtcattaag tcctgatgtc cttgccatca gaccggagaa
     3181 gcaaccccac atatcagaca gtgagaagaa ttttcctcca acctggtagt cgaagtttgc
     3241 cgtaacggaa atgtccttta gaatcttgaa ggagttctgg atacctccgg tatatttcgg
     3301 caatacattt ccgtaatatt tctcagcttc ctttacatag ctcccactct tagtaagata
     3361 tggccttccc ttttcgtcaa ctttcatgcc gcttccgaag atttcacccc atggtttatt
     3421 gattgcatga accaggtaag gagtatccca tccctgattc tgaactgtaa ttctgtcaat
     3481 tccttcagca atctttgtaa cttcctgctt gatcaggtaa gcccatgtgg tatttatttc
     3541 ccatgagaat tttggcgtat caaacggttt gaaattcaag gatagatcaa tacctttctt
     3601 gatcatctca cctgtattaa ccagttttgt agtaaatccg ctgaagcttg atattgttac
     3661 tgatacagga atgtcccttt cagatccatc ccagtatgtt gcagtcagac cgattctgct
     3721 cttcaggaat ctgagttcaa gccccagttc tttctgcgta tttactgcac catgaatatt
     3781 cgggtcaaca agcctgtcgg gtgttcccat caggaagttg ccggtccatt gatactgacc
     3841 tacgccatat tcgaaacctg gatattcgta tactccaatt gatctgggaa tttcacccca
     3901 cgatgcacgg attttaccaa agttaaggaa gggtatttgc agcaggtcac tgaaaacaaa
     3961 cgaacctccg aatgattttg aaagaattga gttattgttt ggaggtaatg ttgaatacca
     4021 gtcgttgcgt agagtgaact ctccgaacag gaaatctctg aatcccacat cacctcttaa
     4081 gaatgctgca cgatacttgt aattctgccg gtcatttaca atattcggct ggctttttga
     4141 attagctatg gtatagaggt ttgctacatt caatccatca accgtattag ccctgttcga
     4201 tttcaggact gaattgaaaa agtcagtacc ggcgtttgcg ttaacattga aatctccgaa
     4261 tttcctgctg aacgacagaa gcgtctcata gttttcacgg ttagaatatg aggtctcagt
     4321 ccggtaatag cctttgtatc taggctcatt tccggttgtt tgcgttccgc tgtagttaag
     4381 gtcggagctg tacttttcct cgtaccatct gttattctgc tgccttctgt aagtaacttt
     4441 gaaattaagt ccttcgataa tcttataact cagggaaaca tctccgaaaa gtctgtctct
     4501 tctttcctgg ttggttacga gatcaaagta tttatagaag ttataccagt agtttgcagc
     4561 ataaaagttc ctgggatttg caggatcgta cacagttggg tcattatggt tccagcttgc
     4621 ccatatacca tcaggggttc ttacatcctt cagttctttc ataattttca tgtcgagatc
     4681 cctgtggaac cactggttga acgaacctgt tgtctggttt gaatatccgt cgtcgaattc
     4741 accgtttgtg aatgttgtaa agaaattcac atttgctgca aaggttaatc tcttggtaag
     4801 gtcgtatgag gtcttaaccg agaagtttgt tctgttcaga taggttgtcg ggataagacc
     4861 atcgacatac aggttaccaa gtgtagcacg gatattgaat ccttctgata cttttgaaaa
     4921 tgcaacactg ttattatatg taaagcctgt ttcataaaag tctcttgcat tattcggctg
     4981 gggtaacagt tttgccgtct tgtatgaata tttatgtcca ccgtaccatg cgtaccatgg
     5041 tatatattcc tgtcctgcca ttcttgggcc ccagctggca tcgtcggaat agttatgata
     5101 atattttccg tcgagtgctt tccattcctc cggatgacca ggtttccact cgtatttctg
     5161 gaggtcgtag tatgcaccac ctgcatagtc attctggtag ttggggagta catatactga
     5221 ggaggcaagg aaaccaagat ttgcctctat tcccattgtc ttaccagcgc ttacccttgc
     5281 tttctttgtt gtaatgatga ttgcaccatt tgcgccctgg tcaccaagga ttgctgaggc
     5341 actcgggccg gagagaacgc tgatatcctc gatatcgtcg aggttcaggt cgttagcatt
     5401 gggcaggatg gtcccatcaa ccacgtaaag gactcccgaa ccttcaccaa aaccgctgcc
     5461 accacgcaac cttatgttac ctgctgcacc cagctttgcg gctgactgtc cctgaaactg
     5521 gataccggat accttaccgg caagtgcatt attaacgttc agcggtcgta cttcattaag
     5581 cctatcacct gaaacaacct gggttaaaga tgaagaactc ttcggagctc tcctgattcc
     5641 ataaccactt gttacaacga tttccgcaag tcccactgtt tcagattcca ttaccacatc
     5701 tacaacagaa cgacctgcaa tctcaacttc aagtgttttc attccgataa aactgaacac
     5761 aagagttttg gcatctacag gtacatttaa ggtgtacttg ccgtttacat cggttgcggt
     5821 accaattgtt gtacctttca ccagaactgt aactccggca agcggtcctt ctccctcaac
     5881 agctgaggta accgtacccg ttatcacccg tgtttgctgc gggaaaagtg ttaaaacact
     5941 tattgcgagc aaaccaatca acagcgatag tttcttcata ctgatttagt ttaaggtttt
     6001 agcaataatg cgctaaatat aaatactgtt tgaattaaat gcaatatttt tgaaaaaaaa
     6061 tttgatttta aaaccattat ataataaata ccggccattc attatcggaa atctctacca
     6121 gttacctttt tttctcatca attctgaagc catactaatc tgcatgtaaa tatcttctcc
     6181 aaaagcacgt tttagagcat ctttaagaaa ttcaaataca tagattcctt ctttcctgcc
     6241 tttttccctt cccggaaaac agatcggcca ttcttccaca ttaacagcag taaaaccttc
     6301 atattttttt attcgtacag ggaacaaatg gcacgataat ggcttgcgaa agtctatttt
     6361 tccttcactc catgcttttt ctatcgcaca tttcagaata ttttcctcca gaatcgtata
     6421 ggcacactcc tgcccgttaa ttaatggagt taccatctct ccttcaaaat cctgcacact
     6481 tgtaccctgt tccgaaattg caatcctgcc ttcatctctc agataaggag agactttatc
     6541 aataatattt tcaagtatat ccacttcatt ctcctgcaac ggagcacctg catcgccata
     6601 cctgcagcaa ttgccaaggc aggcattaag atcacatgca aactttttct gcagcaggtc
     6661 cagactgaat atatattcac ctatgcgaag catcaggtag aacgaaacag ggttattcca
     6721 caagtatttt accggtcatc tcctgaggaa tgggaatacc cattatagta aggatggttg
     6781 gagctacatc ggcaagaatg ccttccttta cctttttata atcatcactt accagtaata
     6841 ccggcacagg gttaagtgaa tgggctgtgt ttggtgtacc gtcttcgttg atagctttat
     6901 cggcattacc gtgatcagca ataatcaaga catcgtatcc gtttttccgt gccgcatcca
     6961 ccacctgacc cacacaatca tcaactgcaa ccactgcttt ccagatagca tcataaacac
     7021 ctgtatgtcc aaccatatcg ccattggcaa aattcaggca tataaaatca tatttacctt
     7081 tatttatttc tgcaataact gcatctttta cctcgtaagc gctcatctcc ggtttaaggt
     7141 cataagtggg aactttggga gatggtatca gaattctgtc ttcacccgga aatacttctt
     7201 ccctgcctcc ggagaaaaag aacgttacat gagcatattt ttcagtttca gctattctga
     7261 gctgtttttt ccctgtcctt ccaagcactt ctccaattgt attatcagca ttctcttttg
     7321 gataaataac atgaagcccc ttaaaggttg catcataagg tgtcattgta caatagtata
     7381 aaggtatggt atgcatccca tattcgggca tatctttctg ggtgagcact attgtaagtt
     7441 cccgggcacg gtcgttgcga aagttgaaga aaataaccac atctccttct ttaagtgttc
     7501 cgaccgggtt tccgttttca tcggtcacca caatcggatt cataaactca tcggttatgt
     7561 cattgtcata cgattcctgt atggcagcaa gaatatcggt tgtctttttt ccctttccgt
     7621 aaactatgag gtcatatgct tcctttatcc tttcccatct tttgtccctg tccattgcat
     7681 aatatctgcc tataaatgaa gcaactctgc cgttcgattt tttcaggtgt tcaagaagat
     7741 tcctcatgta accaagtcca ctacgcggat cagtgtcccg tccgtcgcca aaaccatgaa
     7801 tgaaaacatt tttcaggccg taatccatgg tcatgtcaca gagataatat aaatgtttgt
     7861 ccagcgaatg gacaccacca tcagagagaa ga
//
LOCUS       DAOSNB010000095         7812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9292_length_7812_cov_4.840660, whole genome shotgun sequence.
ACCESSION   DAOSNB010000095 DAOSNB010000000
VERSION     DAOSNB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9292_length_7812_cov_4.840660"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLG42_05320"
     CDS             complement(<1..293)
                     /locus_tag="PLG42_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30661.1"
                     /translation="MKIDVPEMEDKDKNINTPAERGFADSELVQLITGFFRSVYKTIV
                     TDRLIKILLIIAKALAAVVSFVLFIAFFLYLTPLIQDSGTRNFRPKNITASSF"
     gene            complement(283..1401)
                     /locus_tag="PLG42_05325"
     CDS             complement(283..1401)
                     /locus_tag="PLG42_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOU30662.1"
                     /translation="MGKKIIRIILALLTSAVIVVLVIFLAVKITPEPPYQEIKTAIQS
                     ISAASAKQASVYSRKLYSEAENALDSAMAVWKRENKKFIFMRNYEKVLEYARLADKKA
                     REAASLSVQNSNDLEHRLGLKIKSLEETKNKIDEFFGKYPLESETRTRLSKGKLMLKE
                     AEIAYEKKQLLQANKKLNDAEYLLNSVYENTLSDIKTWFASLSTWKKWVDNAITESGK
                     RKSYLIVIDKFAHKFYLYFAGKLKYEFDAELGKNWTGDKRIKGDKATPEGRYKIIKKL
                     QGRATRWYKSLAIDYPNSEDVERFMKEKASGTISSAANIGGGIEIHGGGGKNTDWTDG
                     CIALTNKNMDIIYPIVQIGTPVVIVGSLRSLDEIMNEN"
     gene            complement(1478..2074)
                     /locus_tag="PLG42_05330"
     CDS             complement(1478..2074)
                     /locus_tag="PLG42_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30663.1"
                     /translation="MKSKVLMGFAAIVMVALMSACGKLPQAQIDEANAAIEAAKAAEA
                     EVYVPADFAALQDSMRAVNAYIESQKSKLFKKYGPAVEDLKAVVTLANTVKENAAARK
                     DEVKKEVETLMTDIKAVIDETSKLFAKAPKGKEGKAALDQMKAEMETVNAAVTEAQSK
                     YDSGAYMDALNKVKAAKETVDRINGELKEAIKKAGGRI"
     gene            complement(2370..5306)
                     /locus_tag="PLG42_05335"
     CDS             complement(2370..5306)
                     /locus_tag="PLG42_05335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOU30664.1"
                     /translation="MKEDFPSGNLMFSLVLTRHRLWGPVLIPYIFHKETQKDYYTIKE
                     VLFPFPSQETVVNLLPEEREVVSIINQYSEREIFKLFSRDKNVKEFIANVTEDHIRDH
                     IRPYIEKRIYKCLEIARNESIPIFYQKSDIKSFHNEDFAEIIQENARPVFRFERGKEY
                     STYNLHLEIEGKPFNLRKYQTEIICNSPCVIRCDYKIIFVSEIDGAKLKPFLTKETVL
                     ISKSSEKKYFSTFVRNAVNLYRVEAKGFSIMEAEPEKEAVLNIETGLKGYPQLVLNFK
                     YSGNSVYADEKETSFTEFNDINGEYFFRKYCRDFDWEDECIKTLGGLGFYSDDRVHFT
                     ISSLTGDSTADFYATIEAVNSCYEELTGAGFLICSKKLDKNYNLGPVKLLIDYRAAED
                     WFDILAHVKFGAFEIPFIKLKRNILEGRREYELPDGTVAILPEAWFARYKHVFEFGSV
                     KNGVLKIHRQHFSIINEIFEEKGRTISPRLKKLLVPESIPSVNPPTGMKTELRKYQLE
                     GLNWLVWLQSAKLGGCLADDMGLGKTVQTLALILFNKENKVDGREMHQRSQLTLFDTE
                     QKGWTSLIVVPASLIYNWENEIRKFTPTLKYFTYKGNQRRKNVSYFGDYDIILTSYHI
                     VRQDIEVFSQFHFHYVVLDESQVIKNPSSMVFRAVSMLKSDYRLVLTGTPIENSLSDL
                     WTQLNFVNPGLLGTLSFFKREYARPVEKMRDRKKEDQLRKIIKPFILRRTKEMVAPEL
                     PPLTEQTVFCDMTEEQAEIYEKEKSAVRNSILKSMEVPGHEQNAIQVLQGLIRLRQLS
                     NHPAMVIDEYSGGSGKFETVIRDIENILAEKHKILIFSSFVKHLMLYADYFDSQKLKY
                     SVLTGSSTDRGSIIEKFQIDPDNRIFLISLKAGGVGLNLTSADYVFLLDPWWNPAAEM
                     QALSRAHRIGQDKSVFVYRYISSSSIEEKIIRLQEKKSKLAETFVQSNNPLKDIDLKS
                     LLEIIE"
     gene            complement(5383..5982)
                     /locus_tag="PLG42_05340"
     CDS             complement(5383..5982)
                     /locus_tag="PLG42_05340"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOU30665.1"
                     /translation="METSFFNSDLFNYLILPLLIFLARICDVSIGTMRIIYVSKGKKN
                     IVPFLGFFEVLIWIIAVSRIMQNLDNYICYIAYASGFATGNFIGMLIEERLAVGTQLI
                     RVITPKGGNELVSLLNANGLGATCVEGHGVRENVQLVYTVVHRNDLSKALDVINVFNP
                     RVFYTIEDVRAVSEGIFPPKKTGNSFPLANILKRWRGGK"
     gene            complement(6118..7089)
                     /locus_tag="PLG42_05345"
     CDS             complement(6118..7089)
                     /locus_tag="PLG42_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30666.1"
                     /translation="MKKSVVILFSIAFIVFSVNAQKVTSYTYKLANGITVKMEKAWPK
                     VWVQQTQEAFSASEQPQSVVVNMRIMGELTKGHTFKLTSGGKDVRLKDASPGTYDLKI
                     TSALSGSPGNIVTDITGVVVKPKMKTSVNVTIYDYQINIEESPLPAKGLASWDFKVNR
                     YKGNNDQTYNQAVPTFYAKGARDKTITPDESSGKSSGKIKPGAYDVLVTVEIPGYNQK
                     IWLENFTMKADVSYKVSINMNAGEVMYAGTSREVTKLHLYPSGTSDKMQGVAKPDRSL
                     EFLVVEPATAKFPCMPGTYDVLINYGNKKYEWRKGIIVRTGTITTVK"
     gene            7410..>7812
                     /locus_tag="PLG42_05350"
     CDS             7410..>7812
                     /locus_tag="PLG42_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOU30667.1"
                     /translation="MKNIYRFLIEKYEKIPGLALATIISSSGSAPQKEGASALFSSNG
                     LEAGTVGGGALEETVKYTAIKSITEKRPVIISVNLENSTEDYESAICGGTVSVLIDPT
                     ILEKPDFITDLKEIIKRNVPFSVVTLIYPAEG"
BASE COUNT         2145 a         1631 c         1416 g         2620 t
ORIGIN      
        1 tcaaagctgc tggcagtaat atttttcggc ctgaagttac gtgtaccaga atcctgaata
       61 agaggggtca ggtacagaaa aaaggcaata aagagtacaa aagaaacaac tgcagcaaga
      121 gcttttgcta taatcagaag aatcttaata agtctgtcag taacaatcgt cttgtaaact
      181 gacctgaaaa aacctgttat caactgaaca agttcagagt cagcaaatcc acgttctgca
      241 ggggtgttta tattcttatc tttatcttcc atttccggta catcaatttt cattcattat
      301 ttcatcgagg cttcttaatg aaccaacaat taccacaggg gtgcctatct gcacgattgg
      361 gtagatgata tccatatttt tatttgtgag ggcaatgcat ccgtcagtcc agtcggtatt
      421 ttttcctcct ccaccatgga tttcaattcc tccgcctata tttgctgcag atgaaatagt
      481 tcctgaagcc ttttccttca taaatctttc aacatcttca ctattaggat agtctatggc
      541 cagagattta taccaacggg ttgccctgcc ctgaagtttt ttaattattt tatatcttcc
      601 ctctggtgta gctttatcac ctttaattct tttgtcgcct gtccagtttt tacccagttc
      661 ggcatcgaat tcatatttca atttaccagc aaaatagaga tagaatttat gtgcaaattt
      721 gtcaattaca atgagatatg atttcctttt tcccgattcg gttattgcat tgtcaaccca
      781 ctttttccat gtgcttagtg aggcaaacca ggtttttata tcgctaaggg tattttcgta
      841 aactgaatta agaaggtatt ctgcatcatt aagctttttg tttgcttgca gaagctgctt
      901 tttctcgtaa gcaatttcag cttctttaag catcagtttg ccttttgaaa gccgggttct
      961 ggtttcactt tcaagaggat atttaccaaa gaattcgtct attttgtttt tcgtttcttc
     1021 aagtgatttt attttcaatc cgagccggtg ttcaaggtcg ttgctattct gaactgagag
     1081 gctggctgct tctctggctt ttttatcggc aagtctggca tattccagca ctttttcata
     1141 atttctcatg aagataaact ttttattttc tcttttccag acagccattg ctgaatcgag
     1201 tgcattttcg gcctcggaat atagttttct ggaatatact gaagcctgtt ttgccgatgc
     1261 tgctgagata ctttgtattg ctgttttaat ttcttgatag ggaggttcag gcgttatttt
     1321 cactgcaaga aaaatcacaa gtaccactat tactgctgaa gtcagcagtg caagaatgat
     1381 tcttataatt tttttcccca tctatataaa tttataaaaa accccgggtt gttttcccgg
     1441 ggaatttcct gcaaaattag ctaaataatt tctcagatta aatacgtccg ccagcttttt
     1501 tgatagcctc tttcagttcg ccattgattc tgtcgacagt ttccttggca gcctttacct
     1561 tgttgagggc atccatatag gctccgctgt cgtatttgct ctgtgcttct gtaacagctg
     1621 cattgacagt ttccatctcg gctttcatct gatcgagagc agcttttcct tccttgcctt
     1681 tcggggcttt agcaaaaagc ttgctggtct catcaataac agccttgatg tctgtcatca
     1741 gagtctcaac ctctttcttt acttcgtctt tccttgcagc agcattttct ttaactgtgt
     1801 ttgcaagggt aaccactgct ttcaggtcct caactgcagg accgtacttt ttgaacagct
     1861 ttgacttctg tgattcgata taagcattta cagctctcat tgaatcctgg agagcggcaa
     1921 aatcggcagg aacataaacc tctgcctcag cagccttagc agcctcaatg gcagcgttgg
     1981 cttcgtcaat ctgggcctgc ggaagcttgc cgcatgccga cataagagca accattacta
     2041 tggctgcaaa acccattaaa actttacttt tcatctttct gaccttttta ctttgtttga
     2101 ctattaaaac tttgtttcag gattttcttt ttgaaaaccg ttgcaaattt aatcattaaa
     2161 tttcgaatca tcaagaaatt ttaatacttt tttaatactg ttttaatatt tttttaatgc
     2221 ttcatgaatt caatcctcac tcctgaatga aattatagaa atctattgtc aacttattag
     2281 tttgattatc tgtattttac taatacaata aggataaaaa tgtctgtaat acttatctta
     2341 tacagaaaaa gaatcaggaa taaatttaat tactcgatta tttccagcag gctcttaagg
     2401 tcaatgtctt ttaacgggtt attgctttgg acgaatgttt cggcaagctt tgattttttt
     2461 tcctgcagcc ttataatctt ttcctcgata cttgaacttg aaatatatct gtagacaaat
     2521 acactcttat cctgtcctat acggtgagcc ctgctcagtg cctgcatttc tgctgccggg
     2581 ttccaccatg gatcaagcag gaaaacgtaa tcggcagagg tcagatttaa ccctactccc
     2641 ccggccttga gtgatatcag aaaaattctg ttgtcagggt ctatctggaa tttttcaatt
     2701 atactccccc tgtcggtact cgatcctgtc aaaaccgaat atttaagctt ctgactgtca
     2761 aaataatcgg catacagcat cagatgcttc acaaatgatg aaaatatcag tatcttatgt
     2821 ttttccgcca gtatattttc gatatccctt atcacagttt caaacttacc tgagccacct
     2881 gaatattcgt ctataaccat tgccgggtgg ttcgatagct gcctgagtct tatcagcccc
     2941 tgaagtacct gaatcgcgtt ctgttcatgc cctggtactt ccatgctttt aagaatagaa
     3001 tttctgacag ccgatttttc cttttcgtaa atttcggcct gttcctcagt catgtcgcag
     3061 aacacagtct gttcggtaag cggcggaagc tcaggagcca ccatctcctt tgtacgcctg
     3121 agaatgaaag gctttattat tttcctgagc tgatcctcct tttttctgtc tctcattttt
     3181 tcaacaggtc ttgcatattc ccttttgaaa aatgacagag tgccaagcag ccctggattt
     3241 acaaaattca gctgagtcca gaggtctgaa agtgagtttt ctattggagt tcctgtcagt
     3301 acaaggcggt aatctgactt tagcattgag actgctctaa aaaccatcga ggaaggattc
     3361 ttaataacct ggctctcatc aaggaccaca taatggaaat gaaactgcga gaaaacttca
     3421 atatcctgac gtacaatatg ataacttgtc aggataatgt cataatcgcc gaaatatgaa
     3481 acatttttcc ttctctgatt gcctttgtag gtaaaatatt ttaacgttgg ggtgaatttt
     3541 cttatttcat tttcccagtt atagatgagt gatgccggta caactatgag ggaagtccat
     3601 cccttctgtt ctgtatcgaa cagtgttagc tgggagcgct gatgcatttc cctgccatct
     3661 actttattct ccttgttaaa aagaatcagg gccagtgtct gaacagtttt ccccagaccc
     3721 atatcatcgg caaggcagcc tccgagcttt gctgactgca gccataccag ccagttaagc
     3781 ccttcaagct ggtattttct cagttcagtt ttcatcccgg ttggcgggtt gacggatggt
     3841 attgattcgg ggacaagcag cttcttaagc cttggactga tggttcttcc cttttcttca
     3901 aaaatctcat ttataattga gaaatgctgc cggtggatct ttagaactcc atttttaaca
     3961 cttccgaact cgaacacatg tttgtatctg gcaaaccacg cttcaggaag aatggctact
     4021 gttccatcgg gcagctcata ttcccttctg ccttcaagta tatttctttt aagtttgatg
     4081 aagggaattt cgaaagctcc gaatttaaca tgagccagaa tgtcaaacca gtcttcagct
     4141 gccctgtaat caatgagaag ctttactgga ccaagattat agttcttatc aagtttcttt
     4201 gaacagatga gaaaaccagc tcctgtaagc tcttcgtaac agctgttcac agcttcgatg
     4261 gtagcataaa agtcagcagt ggaatcgcct gtaagggaag atattgtaaa atgaaccctg
     4321 tcatcagagt agaacccaag tcctccaagt gtctttatgc attcatcctc ccagtcaaaa
     4381 tcacggcagt attttctgaa aaaatattcg ccattaatat cattaaactc agtaaaagag
     4441 gtttccttct catcggcata aacactattt cctgaatatt taaaattcag tacaagttgc
     4501 gggtagccct tcagtccggt ctcaatattt aaaacagctt ctttttctgg ttcagcttcc
     4561 ataatcgaaa atccctttgc ctctacccgg taaaggttaa ctgcatttct gacgaaagtg
     4621 gagaagtatt tcttctcgga gctttttgaa atcaaaaccg tttccttggt caggaatggt
     4681 ttcagctttg ctccgtcaat ttctgaaacg aaaattattt tgtaatcaca tcttattaca
     4741 catggtgaat tgcagataat ttctgtctga tattttctta aattaaaagg tttcccttca
     4801 atttcaaggt gcaagttata ggtgctgtat tccttacctc tctcaaatcg gaaaacaggc
     4861 cgggcatttt cctgtataat ttctgcaaag tcctcattat gaaacgattt tatatccgat
     4921 ttctggtaaa atattgggat agattcattt ctggctattt caaggcactt ataaattctt
     4981 ttttcgatat aggggcggat atggtctctt atatggtctt cggtaacatt ggcgataaac
     5041 tctttaacgt ttttatcccg ggaaaaaagt ttgaaaattt ctctttcgga gtattgatta
     5101 ataatcgaaa ctacttccct ttcttcaggc agaagattca ctacggtttc ctgtgaggga
     5161 aaaggaaaca gtacttcctt tattgtataa taatcttttt gtgtctcttt atggaatata
     5221 tatggtatca gcaccggtcc ccataacctg tgccgggtaa ggacaagtga aaacatcaga
     5281 ttccctgaag gaaaatcttc tttcaattat taatgaatta aataaatctg caattttatt
     5341 aatcttcgtg gtattgtcaa actatttttt tcagaattga tatcattttc ctccgcgcca
     5401 ccgtttcagt atgttcgcca gaggaaatga attgcccgtt ttcttgggag gaaagattcc
     5461 ttcgcttaca gctctgacat cttcaattgt ataaaagact ctggggttga aaacatttat
     5521 tacgtctaaa gcttttgaca gatcattcct gtgtacaacg gtatagacca gctgaacatt
     5581 ttcacggaca ccatgacctt caacacatgt agctcccagt ccgttggcgt taagaaggct
     5641 taccagttcg ttacctcctt tcggtgtgat aacccttatc agctgcgtgc caactgcaag
     5701 cctctcttct attaacatac ctataaaatt acctgtggca aaacctgaag cataggcaat
     5761 ataacagatg taattatcaa ggttctgcat tatcctgctg acagcaataa tccagatcag
     5821 gacctcgaaa aatccaagaa aaggtactat gttcttttta ccctttgata catatattat
     5881 tctcattgta ccgattgaca catcacatat tcttgccaga aaaatcagca atggcaaaat
     5941 cagataatta aacagatcgc tgttgaaaaa tgaagtttcc ataccttaaa aatattttat
     6001 agcaaagatg cacccaaatg tgaaatctgg caatgattta tgaaataaaa aaaggagtcc
     6061 ggcagtgttg atggttaccg gacttcttat tgtaagaaat atttgaattt aattttgcta
     6121 tttaaccgtt gttatcgttc cggttctgac tattattccc tttctccatt cgtatttctt
     6181 attaccataa ttaatcagaa catcatatgt tccgggcatg catgggaatt ttgcagtggc
     6241 gggttcgaca acaaggaatt caagcgacct gtcaggttta gctacgccct gcattttgtc
     6301 ggaagtgcct gacggataga ggtgaagctt tgtaacttcc ctcgaagtac cagcatacat
     6361 aacttctccg gcattcatat ttattgagac cttatagctg acatccgctt tcatggtgaa
     6421 attctcaagc catattttct ggttatagcc gggtatttca actgtaacca gcacatcgta
     6481 tgctccgggc tttattttcc ctgagctttt acctgacgat tcatccggag ttatggtttt
     6541 gtctcttgca cccttggcat agaatgttgg cacagcctga ttgtaagtct ggtcgttatt
     6601 tcctttatac ctgtttacct tgaaatccca gctggcaagt ccttttgcag gaagaggtga
     6661 ctcctcaata tttatctggt aatcgtagat tgtaacattc acgcttgtct tcatcttagg
     6721 cttaaccacc acaccggtta tatctgtaac aatattaccc gggcttcctg aaagtgccga
     6781 agtaatcttc aggtcatatg tgcctggtga agcatctttt aacctcacat ctttaccacc
     6841 gcttgtgagc ttgaaagtgt ggcccttcgt aagctctccc ataattctca tgtttacaac
     6901 caccgactgt ggctgctcag aggcactgaa agcttcctgc gtctgctgaa cccatacctt
     6961 gggccatgct ttttccattt ttacagtgat accgttagcc agtttgtatg tataactggt
     7021 aactttttgt gcattgaccg aaaaaacgat aaacgcaata gaaaacaaaa tgacaacact
     7081 ttttttcata gctgtatttt gtttgttatt gtaactgttt taccaaattt aactcttttt
     7141 tttgattcag ggtattaatt tcctccataa aaacatgctc cgaaaacatt ttttaacaaa
     7201 tttggacaat aatatttctt atccatgatt ttaatatttt aacaaacaaa agagtcggtt
     7261 tttgatattt ttaattataa gaaccagata gtaaaaaatg attaaaaaac tatcccttca
     7321 gtaaatttat tctctgtaat gataagtaaa atcacttttt tgaatatact tcttattttt
     7381 gaccatatcc ggtaaaaaac aggcgaagga tgaaaaacat ttacaggttt cttatcgaga
     7441 aatatgagaa aatacccgga ctggctctgg cgaccataat atcatcatcc ggttcggcac
     7501 ctcagaaaga aggggcttca gctcttttca gttccaatgg gcttgaagcc ggcacagtgg
     7561 gtggtggtgc tctggaagaa acagttaaat atacagctat taaatcaatt accgaaaaaa
     7621 ggccagtaat tataagtgtt aatctggaaa acagcactga agattacgaa tctgccatat
     7681 gcggaggcac tgtcagtgtt ctgattgatc cgaccatact ggaaaaaccg gacttcataa
     7741 cagatcttaa agagattatt aaaagaaatg tgccatttag cgtcgtaact ttgatatatc
     7801 ctgctgaagg ag
//
LOCUS       DAOSNB010000096         7805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9309_length_7805_cov_4.417032, whole genome shotgun sequence.
ACCESSION   DAOSNB010000096 DAOSNB010000000
VERSION     DAOSNB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9309_length_7805_cov_4.417032"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..3738
                     /gene="mfd"
                     /locus_tag="PLG42_05355"
     CDS             409..3738
                     /gene="mfd"
                     /locus_tag="PLG42_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOU30668.1"
                     /translation="MRENQLSLIYDNHEGVTLLKKRISEEETGRIVLKGLAGSSVAMV
                     ISSVYRACPLINLVIAPEKEEAAYFYSDLVSLLGDDSVFFFPSTYKRSIIYEQTDPAG
                     IILRTEVLNFLASGKNNGLIVTYPEALMEKVVSKQTLSDNTFTVKKGERLSVDFLEEL
                     LQEYGFERVDFVYQPGQYAIRGSIADIFSFSADMPFRLDFFGDEVESLRSFNPDDQLS
                     VSMLDEVKIIPDINSLPENKTTQTFIDLLPRTSLIWTEDLDFTAGRIDAIFRQAAEKE
                     RNGLLDGLKEKVITGGMLINELGTFKIIETGRLETPENVTPIFFRTEPQPSFGKNFEL
                     LSKQLITNQENGYETVIVTENQSQTERLNDIFAEICPGVSYSTVIKSLHRGFTDHDLR
                     IALYTDHQIFDRYHKYRIKGYFTRKESITIKEITGLNPGDYVVHIDHGIGRFGGLEKI
                     EVNGKIQEAIKLVYKDNDILYVPIHSLHRISKYRSSDGQEPRISRLGSGAWQRLKDRA
                     RNKVKDIAKDLIKLYATRMASPGYSFSPDSYLQRELEASFLYEDTPDQLSATIDVKRD
                     MESPHPMDRLVCGDVGFGKTEIAIRAAFKAAADNKQTAVLVPTTVLAYQHYRTFTDRL
                     KEFPCTVEYLTRHKKPAEQKKILEKLEKGQIDIIIGTHKLISDNVKFRDLGLLIIDEE
                     QRFGVAAKEKLKKLKASVDTLTLTATPIPRTLQFSLMGARDLSIINTPPPNRMPIVTE
                     LHVFNEEIIKEGIEYEVSRGGQVFFIHNRIDNIGEIQALIHRLCPAVKTAVVHGRMDG
                     RTIENTMLDFISGDYDVLVATTIIESGLDIPNANTIFINNAHYFGLSELHQLRGRVGR
                     SNRKAFCYLLAPPPETLTNEARRRLKAIEEFSELGSGFSIALQDIDIRGAGNLLGSEQ
                     SGFIAEIGFETYQRILNEALIELRESEGLTDLSRTVAEPGKEKAFVKDCQIDTDFEIM
                     FPDEYISNISERLRLYKELNEITSETGLAEFERKLTDRFGPLPRPAKSLLELVKLKWL
                     ASSSGIEKIILKNNLFVANFISDPGSSFYRSQLFATMISRINSFPRRMKVKQKDLKLT
                     LTVSEVKSVEEAMDVLRKLTE"
     gene            3805..4524
                     /locus_tag="PLG42_05360"
     CDS             3805..4524
                     /locus_tag="PLG42_05360"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOU30669.1"
                     /translation="MNLVVDIGNTSIKLAIYGPQGKIAIRKFDILDEAAFDNFLKEYD
                     TEKVIVSSVRKVPEKFSEYFKKRAKFFHFLSSQSKLPFPVDYETPETLGTDRIAAVAG
                     AYKAFQNKNCLIIDAGSAITYDFMIYGRFIGGNISPGISMRFKALNTFTDALPLVKEW
                     DEFSFPGVNTTDAIAAGVISGVIYEINEYIRTFEKKYKNTVIILTGGDGKFLNSKIID
                     KPVYMPDLVIDGLNFILEYNV"
     gene            4517..5812
                     /locus_tag="PLG42_05365"
     CDS             4517..5812
                     /locus_tag="PLG42_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30670.1"
                     /translation="MYRKAVYIIISFVITFSNLHGQKLINSPYARFNIGILNPQGTLR
                     SLGMGGISTAMRDNNTISFSNPASYTSFDTTSFVFDMGMDLSRLKLLNGNASFSSTDM
                     NFRHLLLGFPISKRIDIAAGLIPFSNGYYYIAEKITKGQPGWNETTGDYSTQHKGSGS
                     ITSLFAGAGVKITKNISLGINITTLFGKLTRLNQYEFAESTTSFNQNSVESFSITGFN
                     ADFGAQYTATIKKDYFINAGISYTPQKNYKSTHEFFKERYTIYRYPPYSPDTLANYGS
                     TTTDSTRLPDVLKLGILFGKKDKIAFGIDYVISGWNNARIHGSDAPMANTRTLMAGLE
                     YIPEKYSNTNFLKRIEYRLGGRISDNYLVLNGVQLKEYSATAGFAIRLKNSYSKATFY
                     FDYTRRQGDLSKGLHNEDIFSVGASLNLYDFWFIKRKYE"
     gene            5813..6406
                     /gene="lptC"
                     /locus_tag="PLG42_05370"
     CDS             5813..6406
                     /gene="lptC"
                     /locus_tag="PLG42_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOU30671.1"
                     /translation="MSVFKLRLSLSNFLTLTFRKNFFIIPVFLFFLSSCERKIETIPK
                     SDIESLPSLIERDFETIYTDSAKLQLVMRSPLMERYTDRKPQYTEFRKGITVIFYDGN
                     PTPIASISSRYARYIEDKKLWELKDSVTAVNEKNETLETELLYWDQEKDIVYTDRFVR
                     IKSEEQIVMGTGFEANSRFTRWKIRNVSATIYLRDEE"
     gene            6396..6581
                     /locus_tag="PLG42_05375"
     CDS             6396..6581
                     /locus_tag="PLG42_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30672.1"
                     /translation="MKSKYLSLILEIVWIIAGVLTFVSGINQMLSDNWKRALLFFAMS
                     VVSFAFARFRHSQRKKL"
     gene            6591..>7805
                     /locus_tag="PLG42_05380"
     CDS             6591..>7805
                     /locus_tag="PLG42_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOU30673.1"
                     /translation="MDYNYIILLLAVVFSAFFSGMEIAFITSNKLRIEIDRKQGLFGA
                     GITKIFIDNPGQFIATMLIGNNICLVVYSIIISKLLEPVISTIVVSDAITLIINTLIS
                     TAIILLVAEFLPKAIFRISPNFFLKTFSVPTFIFYLLFYPVSKFTLFLSNAILSIVPG
                     GKVKKKENEEELVFSKADLDHFVNVVSHGEIKDESDRENIKIFQKALDFSNIRVRECM
                     IPRTDIEAVEIGTSIDALRERFIATRYSRILVYTDTIDQIAGYVELKDIFKNPPDISS
                     ILRKIVIVPETMPANRLLELFIDEKVNIAVVVDEFGGTSGLITIEDVVEEIVGDIEDE
                     HDVSELVEKQVSENEFVFSGRLEIDYIREKYSLNLPEEDDYETLAGMVLYYHGNIPKS
                     NDIIVFFWKIQTI"
BASE COUNT         2492 a         1445 c         1608 g         2260 t
ORIGIN      
        1 tataaagaaa ctggaaaata tatcatttct ctgtcaactt ttttcaggac gatatgaaaa
       61 gatacctccc tttgtatttc cttgcagcat aaactgaccc ccctttgtat ttccccccac
      121 ggggggaaaa attattgtaa taatctgttt ttcaaatatt ttttaattat tggcaataca
      181 ttttcagtgt catttggcat tatccctggg gttgggtgat aagccccctc tttacgaagt
      241 agagaggggg ttgggggtga gtacattgtg tcaaaataaa gagaaaagcc aatccaattt
      301 ttgtcaactt atttcagaac aaaacatagg accattgaca atatattatt ttgcctgtca
      361 ggtataattc cacgccactc tttgtaaatt tgcgccggat tttataaaat gagagagaat
      421 caactgtcat tgatatatga taaccatgag ggggttacac ttctgaaaaa aagaatttca
      481 gaagaagaaa ccggcagaat agtccttaag gggctggcag gctcttcagt tgccatggtt
      541 atttcgtcgg tttaccgggc ttgtcccctg ataaatctgg tcattgcccc ggaaaaagag
      601 gaagctgcat atttttatag cgacctcgtt tctttactcg gagatgactc tgtattcttc
      661 ttcccttcaa catataaaag gtcgattatt tacgaacaga ctgatccagc aggtatcatt
      721 ctcagaacag aagttctcaa tttccttgct tcaggtaaaa ataatgggct gatagttaca
      781 tatcctgaag ctttaatgga aaaagtagtc agcaaacaga cccttagcga taatacattt
      841 accgttaaaa aaggagagag gctttcggtt gattttcttg aagaattgct gcaggagtat
      901 ggttttgaaa gagttgattt tgtttatcaa ccgggtcaat atgcaatcag gggcagcata
      961 gctgatatat tttctttttc tgctgatatg cctttccgct tagatttttt tggcgatgag
     1021 gttgagtctc tccgaagttt caatcccgac gaccagcttt ctgtaagtat gctcgatgaa
     1081 gtaaagatta tacctgacat aaattccctc ccggaaaata aaaccacaca gacttttata
     1141 gatttgctac cccgcacttc tctgatatgg acagaggatc tggattttac agctggcaga
     1201 attgacgcta tcttcaggca ggcggccgaa aaggaaagaa acggattact tgatggcctg
     1261 aaagaaaaag ttataacagg agggatgctg atcaatgaac ttggtacgtt caaaataatt
     1321 gaaacaggca ggcttgaaac tccggaaaac gtaacaccta tatttttcag aactgaacct
     1381 cagccatcgt ttggtaaaaa ctttgaatta ctcagcaagc agcttattac caatcaggaa
     1441 aacggatatg aaacggtaat agttaccgaa aatcaatcac agaccgaacg tctgaacgac
     1501 atctttgcag aaatatgtcc tggagtaagc tacagcactg ttatcaaaag cctgcataga
     1561 ggttttaccg accatgattt gagaattgca ttatacactg atcatcagat atttgatagg
     1621 tatcataaat acagaatcaa aggttatttc acaaggaagg agagtataac cataaaagag
     1681 ataacaggat tgaatcctgg tgattatgtg gtacacattg atcatggtat cggaaggttt
     1741 ggcgggcttg agaagattga agtaaacggc aagattcagg aagccatcaa actggtttat
     1801 aaggataatg atatcctgta tgttccaatt cattcgttac acaggatctc taaatacaga
     1861 agcagtgacg gacaggagcc gcgcatcagc aggcttggtt cgggtgcatg gcaaaggctg
     1921 aaggacaggg caagaaacaa ggttaaagat attgcaaaag accttataaa actttatgca
     1981 acaaggatgg catcgccggg ttattccttc tctcccgatt catacctcca gagggaactg
     2041 gaagcctcgt ttctgtacga agacactccc gaccagctat cagcaactat tgatgtgaaa
     2101 agagatatgg agtcgcctca tccgatggac aggcttgttt gcggcgatgt cggattcggt
     2161 aagacagaga tagccatcag ggctgcattc aaggcagcag ccgataacaa acagactgcc
     2221 gtacttgttc caaccactgt ccttgcctat cagcactaca gaacttttac cgaccgactg
     2281 aaagagtttc cctgcactgt cgaatatctg acccgccata aaaagcctgc agagcagaaa
     2341 aaaatacttg aaaaactgga aaaggggcag atcgatataa ttattggaac tcataaactc
     2401 atcagcgata atgtgaaatt cagggatctt ggattgctga ttattgatga ggagcaaaga
     2461 ttcggggttg ctgcaaagga aaaacttaaa aaattgaaag ccagcgtaga tactcttact
     2521 cttacggcaa cgcctatccc gcgcacactt cagttttcac ttatgggagc ccgcgacctt
     2581 tccataataa acacaccccc tcccaacagg atgcccatag taacagagct gcatgtcttc
     2641 aatgaagaaa tcatcaaaga agggatcgaa tatgaggtaa gccgcggtgg ccaggtattt
     2701 tttattcata acaggattga taatattggg gagattcagg cacttattca ccggttgtgc
     2761 cctgcagtta aaactgccgt agtccatggc agaatggatg gtagaaccat agaaaatacc
     2821 atgcttgatt ttatcagcgg cgattatgat gtgcttgttg caacaacaat aatagaatcg
     2881 ggcctcgaca tacccaacgc caatactatt ttcatcaaca atgctcatta ttttggcctt
     2941 tcagagcttc atcaactcag gggaagagtg ggtcgctcaa accgcaaggc tttctgctat
     3001 ctgcttgcgc ccccgccaga gactcttaca aatgaagccc ggcgcagact gaaagcaatt
     3061 gaagaattct cggagctggg cagcggcttc agcattgccc tgcaggatat tgatatacgt
     3121 ggtgcaggaa atctgcttgg cagtgaacag agtggcttta ttgcggaaat aggatttgaa
     3181 acataccagc ggatattaaa tgaagctctc atagaattaa gagagagtga aggccttact
     3241 gacctgagca gaacagttgc agaacccggg aaagaaaaag cctttgtgaa agattgtcag
     3301 atagataccg attttgaaat tatgttcccg gatgagtata tatcgaacat ttcggaaaga
     3361 cttagactat acaaagaact taatgagatt acatcggaga caggtctggc agaatttgaa
     3421 agaaagctga ccgacaggtt tggtccttta ccccgcccgg ctaagtcact tctcgaactt
     3481 gttaaattaa aatggcttgc atccagttcc ggaatagaaa aaataatatt gaaaaacaac
     3541 ctgtttgttg caaatttcat ttccgacccg ggctcgtcat tctatagaag tcaactattt
     3601 gccacaatga tttcaagaat aaacagcttt ccccgcagga tgaaggtaaa gcaaaaagat
     3661 ttaaaactta cacttaccgt ttctgaagta aaatcagtgg aagaagcgat ggatgttctc
     3721 aggaaactaa ctgaataata attctttata aaaaatatag cgtacaattg ccataaattg
     3781 ttaattttga ggcaaattgt tttgatgaat cttgttgtag acataggtaa cacttcaatc
     3841 aagttagcta tttacggacc acaagggaaa attgccatca gaaagttcga cattctcgat
     3901 gaagcggcat ttgataattt cttgaaggaa tatgatacgg agaaagtaat tgtttcgtcg
     3961 gtaagaaagg ttccggaaaa attttcagaa tatttcaaaa agagagcaaa attcttccat
     4021 tttctgagct cacagtcaaa actccccttt cctgttgatt atgagactcc tgaaacactt
     4081 ggaactgacc gtattgcagc cgttgcgggt gcttataaag cctttcaaaa caaaaactgt
     4141 cttataatag acgccggaag tgcaataact tacgacttca tgatttatgg caggtttatt
     4201 ggaggtaata tttcaccggg tatcagtatg agattcaaag cactcaacac ctttacagat
     4261 gctctacccc ttgtgaaaga atgggatgaa ttttctttcc cgggggtaaa tacaactgat
     4321 gctattgctg ccggggttat cagcggagtt atttatgaaa taaatgagta tattcgcacg
     4381 tttgaaaaga aatataaaaa cacggttatc attttaacag gcggcgacgg taaatttctc
     4441 aatagcaaaa taatagacaa accggtatat atgcctgatc tggttattga tggccttaat
     4501 tttattctgg aatacaatgt ataggaaggc agtatatatt ataatttctt ttgttataac
     4561 tttttcgaat ctgcatggac agaaactcat caactcgccc tatgcccggt ttaatattgg
     4621 catccttaat cctcagggga cattaagaag cctgggaatg ggaggtatta gcacagcaat
     4681 gagggataat aatactattt ccttcagcaa tccggcttct tatacatcat ttgatacaac
     4741 atcatttgtt tttgatatgg gaatggatct gagccggctt aagcttttaa acggaaatgc
     4801 cagtttctct tcaacagata tgaatttcag acatcttctg ctcggtttcc caataagcaa
     4861 aagaattgat attgctgcag gacttatccc attcagcaat ggttattact atatagctga
     4921 aaaaattact aaagggcagc caggatggaa tgaaacaaca ggagactatt caactcaaca
     4981 taaaggttca ggcagtataa catctctttt tgcaggagct ggtgttaaaa tcactaaaaa
     5041 tatttcgttg ggtatcaata taacaactct ttttggcaag ctcacgcgtc tgaaccagta
     5101 tgaatttgct gaaagtacaa cttcattcaa tcaaaatagt gttgaaagtt ttagtataac
     5161 tggattcaat gccgattttg gtgctcaata tacagcaact attaaaaaag attacttcat
     5221 taatgccggt atttcttata ctcctcaaaa aaactacaaa tcaactcatg aatttttcaa
     5281 agaacgttat actatttaca gatatccccc atattcacct gatactctgg caaattatgg
     5341 ttcaaccaca actgattcca caaggctccc tgatgttctg aaacttggga ttttattcgg
     5401 aaagaaagac aaaatagcct ttggcatcga ctatgttata tcaggatgga acaatgcaag
     5461 aatacatggt agtgatgctc ccatggcaaa tacccgtacc ctgatggctg gtctggaata
     5521 tattcctgaa aaatattcaa atacaaattt cctgaagaga attgaataca ggctgggtgg
     5581 acgcatatcg gacaactatc ttgtgctgaa tggagtacag ctaaaagaat acagtgccac
     5641 tgcaggcttt gcaataagac tgaaaaattc ttattcaaaa gccactttct attttgatta
     5701 cacaagaaga cagggggatc tttcaaaagg tcttcacaat gaagatatat tttctgtcgg
     5761 tgcaagtctg aatctgtacg acttctggtt cattaaacgc aaatacgaat aaatgtctgt
     5821 ttttaaactc agactatcat taagtaattt tcttacttta acattcagga agaatttttt
     5881 tataatcccg gtattcctgt tttttctttc ttcatgcgaa agaaaaattg agacaattcc
     5941 aaaatcagat attgaatcat taccatcact gattgagagg gattttgaaa caatttatac
     6001 cgattcggct aaattacagc tagttatgag gtcacctctc atggagaggt ataccgaccg
     6061 caagcctcag tacaccgagt tcagaaaggg catcacagta atattctacg acggaaaccc
     6121 cactcctatc gcctctatct caagcagata tgccagatat atagaagata agaaattatg
     6181 ggagctaaag gacagcgtaa ctgcagttaa cgaaaagaat gaaactcttg aaacggaact
     6241 tctgtattgg gatcaggaaa aagatattgt ttacactgac cgttttgtta gaataaagag
     6301 cgaagaacag attgtaatgg gtacggggtt tgaagcaaat tcacgtttca ccagatggaa
     6361 aataagaaat gtaagtgcaa ccatttatct gagagatgaa gagtaaatat ctttcactga
     6421 tactggaaat tgtctggatt attgctggtg ttctcacatt tgtttccggg ataaaccaga
     6481 tgctgtcaga taactggaaa agagcattgt tgttttttgc aatgtcggtt gtttcttttg
     6541 cctttgcccg tttcagacat tctcagagaa aaaagctgta attttgcagt atggactata
     6601 attatattat actcctgtta gctgtagttt tctcagcatt tttctctggc atggagattg
     6661 cctttataac ttccaataaa ttaaggattg aaatcgacag aaagcagggt ctgtttggtg
     6721 cgggcattac aaagattttt attgataatc ccggtcaatt cattgcaacc atgcttattg
     6781 ggaacaatat ttgtctcgta gtttatagta ttatcatatc gaagctgctt gaaccggtaa
     6841 taagtacaat agttgtttca gatgctataa ccctcataat caataccctt atttcgactg
     6901 caatcattct gcttgttgca gaatttcttc ccaaagctat atttcgcatc agcccgaact
     6961 tctttctcaa aacttttagt gttccgacat ttatcttcta tcttcttttt tatcctgttt
     7021 caaaatttac tcttttcctt tctaacgcaa ttctcagtat agttcctggc ggaaaagtga
     7081 agaaaaaaga aaatgaagaa gagctcgtat tcagtaaggc agatcttgat cattttgtaa
     7141 atgtagtttc gcatggtgaa ataaaggatg aatcggacag ggaaaatata aagattttcc
     7201 agaaagcgct cgatttttct aatataaggg tgcgtgaatg catgataccc cgaactgata
     7261 ttgaggcggt tgagataggc accagcattg acgcgttgcg tgaaaggttt attgccacaa
     7321 gatactcgag gattctagtt tatacagata caattgacca gattgccggg tatgttgaac
     7381 tgaaggatat atttaaaaat ccaccggata taagttctat tctaagaaaa atagtaattg
     7441 ttccggagac tatgcctgca aacaggttgc tagagctttt tattgacgaa aaggtaaaca
     7501 ttgccgtggt ggttgatgag ttcggtggta catcagggtt gattacgata gaagatgtcg
     7561 ttgaagagat agtaggtgac attgaagatg agcatgatgt atctgagctt gtagagaagc
     7621 aagtaagtga aaatgaattt gtcttttcag gcaggcttga aattgattat atcagggaaa
     7681 aatatagtct gaatcttcca gaagaagacg attatgaaac acttgcaggg atggttctct
     7741 attaccatgg taacataccg aaaagcaatg atataatcgt cttcttctgg aagattcaga
     7801 ctata
//
LOCUS       DAOSNB010000097         7795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9330_length_7795_cov_4.288372, whole genome shotgun sequence.
ACCESSION   DAOSNB010000097 DAOSNB010000000
VERSION     DAOSNB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9330_length_7795_cov_4.288372"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLG42_05385"
     CDS             <1..765
                     /locus_tag="PLG42_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOU30674.1"
                     /translation="GDFFGMGWGTYAPLSSLAVCVNPGSAFNCYWVMPFRKKCKITME
                     NISDTDPMTLYYQIDYTLTEVPADAAYLHAQFRRANPNETSVYTIVDGIKGKGHYVGV
                     YMAWGVHNNGWWGEGEIKFYLDGDTDYPTICGTGTEDYFCGSYDFDTRKKNAYGVEEV
                     NYTEFCTPYSGLHQVIRGDGHYNVMQRFGLYRWHIMDPIRFEKDLKVTIQDLGWRRGG
                     RYLAQKSDISSVCFWYQTEPHAKFPKLPDWRGLEIY"
     gene            816..2165
                     /locus_tag="PLG42_05390"
     CDS             816..2165
                     /locus_tag="PLG42_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU30675.1"
                     /translation="MKKKIEKSRRRFITTSAVAAIGALGAGRILTSCSESGSSEKIKA
                     LPPMPEKAPDGRPLKAGLIGCGGRGTGAAINFLDAGNGLEIAALGDVFQDRLDNCRAE
                     LKKQRNVEVPDEKCFVGFDAYEKVIDSGVDIVLLCTPPHFRPAHVEAAVNAGKHIFME
                     KPVAVDPVGARSMMISAEKAKQKGLCMVSGTIRRVQKDFMETQRRVANGEIGEIVSAH
                     IIRNGGALWVRKRQPGWTDMEYMLRNWANFCWLSGDHIVEQFIHEIDVMNWFIGKYPV
                     KAIGWGGRQRRVTGDQYDFFSIEYIYDNGMHTHCAARQINGCTNLTKQFFAGTKGYAN
                     AKGEIYNLKGEKIWSYPYPEKDSPDQTWKVNDPFVQEHVNLVTAIRTGNVYNDADAQI
                     KSTITCIMGRMSAYTGKDVTWEEVMNSDLYLGPKTYAFGPVPGIEEKPPVIGIEPKI"
     gene            2296..3192
                     /locus_tag="PLG42_05395"
     CDS             2296..3192
                     /locus_tag="PLG42_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU30676.1"
                     /translation="MNRRTFTKTVAAATVAILAFRLSSFSTTHPASARNLKKGVMWGS
                     IAYAKTVREKFLAAKEAGFDGVEVNSHLDRKEVLEASRETGLPVASVCGALHWKYLLS
                     DPDPKIREQGIEALRVTIEDAKTYGTDTVLLVPGRVTETVSYDECWNRTVAEIKKILP
                     FAEKSGVTIAIENVWNNFLLSPLEAVRYVDQFKSKRVGFYFDVGNIVAYGWPEQWIRI
                     LGKRIARVHIKEYSRKIADSQGRSAGFKVNLMEGDVNWPAVMKALDEIGYSGWTTIEQ
                     PGGNSPEGLKDLCDRLVKIQGS"
     gene            3344..5851
                     /locus_tag="PLG42_05400"
     CDS             3344..5851
                     /locus_tag="PLG42_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOU30677.1"
                     /translation="MKTQILIILIFSLLASCSEARKKTSGHRYNDWYYGENLSRIAFP
                     VGGIGAGMFCIEGTGAISHMSVRNRPEVFNEPCMFAALSVEGIENGTKILEGPVPDWK
                     KFGRPGTGNGASGTSYGLPRFQNAKFISRFPFATIVLQDDDIPLDIELTAWSPFIPTD
                     ADNSSLPAGAVEYKFTNTGNKSIHAVFSYSSKNFMAQTGGMNSIKPLAGGFILSEEGI
                     KERPWTKGDFAIFTDDPATVVDYCWFRGGWWDPLTMAWKTISSTETLSRKPVEKDAPG
                     ATLFVPFDLAPGETKSVNLMFSWYVPESNIRYGSDSQEPGVTRSEDPAAPENQMYYKP
                     WYSGRFSSIVEVSDYWKENYAALKNKSELFRDAFYGQTLPSEVIEAVAANLTILKSPT
                     VLRQTDGKLWCWEGCSDNSGCCAGSCTHVWNYAQAIPQLFPSLERTLRETEFFKSQNS
                     EGHQTFRSALPIRPVAATFYAAADGQLGGIMKVYRDWRISGDNDWLKMIFPKVIQSID
                     YCIRTWDPRHTGTLEEPHHNTYDIEFWGPDGMCTSFYLGALRAVCEMGKYLGENISGY
                     EELYLKGRKFMEEELFDGEYFIQKIMWEGLNAPNPVEASKGAWNTDYSDEARELLQKE
                     GPKYQYGKGCLSDGVLGAWIGEVCGLHNVIDSTKVRSHLNSVFRYNFRSDLSDHANPQ
                     RPSYALGNEGGLLLCSWPRGGALSLPFVYSNEVWTGIEYHVASHLMMMGETEKALKIV
                     RACRDRYDGRVRNPFDEYECGHWYARAMSSYGLLQGLTGVRYDAVTRILYLKAGIGDF
                     TVFISTETGFGKAGIKKGKPFCEVVYGKIEIKEYKLI"
     gene            complement(5869..7128)
                     /locus_tag="PLG42_05405"
     CDS             complement(5869..7128)
                     /locus_tag="PLG42_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30678.1"
                     /translation="MKNLIKENIENPEQLEKLYRFDRKGFAKAFSEIYPEISEHPASA
                     FWKARLEPDTVKADWLITVKKDLLFLLVTCALAGFFIKFPAIFRTEADMDAFYIKNAG
                     LVVIGALTLYYLLTVKGFKVVHLIFTTLIFFVSAIYINLLPGDVHLNTVLLACLHLPV
                     ILWFIYGLVYSGFNLREKSGWINYIRYNGELAVMFGIIAIAGGILTAVTIGLFDVINI
                     NAERFYSDYVVIIGAVSAPVAATFLIRYFPAITSKIPPVIASIFSPLVLLTLIIYLLV
                     ILLSGKSLYTNRDFLLVFNVMLLGVTAIIVFSVSETTFFFRQRLLEVILLALVIFAII
                     TDLLALSAIIYRLGEYGFTPNRTAVLGSNLLILGNLVLFARDLYRVNFKKDSAGILEN
                     TIAKYLPVYAIWAVIVVFIFPVIFGLR"
     gene            7579..>7795
                     /locus_tag="PLG42_05410"
     CDS             7579..>7795
                     /locus_tag="PLG42_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30679.1"
                     /translation="MFLQNPDSIEWEVAPSVYFDTPRSGKGDTVVNINPYPGYIGKQD
                     EIKFTLHQNINVVNISWRFPILQTAMWI"
BASE COUNT         2383 a         1729 c         1774 g         1909 t
ORIGIN      
        1 ggcgatttct ttggaatggg atggggaacg tatgcaccat tgtcatcact tgcagtgtgc
       61 gttaacccag gtagtgcttt caattgctac tgggttatgc ccttccgcaa gaaatgcaaa
      121 atcaccatgg aaaacataag tgatacagat cccatgacac tttattacca gattgactat
      181 acccttaccg aagtgcctgc cgatgcggct tatctgcatg cccagttccg ccgtgcaaac
      241 ccgaacgaga cttccgttta tactattgtt gatggaataa agggaaaagg acactatgtg
      301 ggagtatata tggcatgggg cgttcataat aacggctggt ggggtgaagg ggaaataaaa
      361 ttctacctcg atggagatac cgattacccg acaatatgtg gcaccggaac cgaagactat
      421 ttttgcggct catacgactt cgacacaaga aagaagaatg cttacggggt cgaggaagtc
      481 aactatacgg agttctgtac cccatattca gggttgcacc aagttatacg cggcgacggg
      541 cactacaacg tgatgcaacg attcgggctt taccgctggc atataatgga tcccatacgg
      601 ttcgaaaaag acctgaaggt cacaatacag gaccttggct ggagaagagg tggccgatat
      661 cttgctcaga aatcggatat ctcttcagtg tgcttctggt accagaccga accccatgca
      721 aaatttccga aactgcccga ctggagaggt ttggagattt attaacatgg taataaaatt
      781 cactattttt aattcaaaat attaataaac ctgatatgaa aaagaaaatt gaaaaaagcc
      841 gcaggcggtt tattaccaca tcggctgttg ccgctatcgg agcccttggt gccggcagga
      901 tactgacttc ctgtagcgaa agtggctcgt cggaaaaaat aaaagcactt cctcccatgc
      961 ctgaaaaggc acccgacgga cgtcctctca aggcaggtct gataggctgc ggaggcagag
     1021 gcaccggtgc tgccattaac ttcctcgacg caggaaacgg acttgagata gctgcactgg
     1081 gcgacgtgtt ccaggacagg ctcgataact gcagggctga actcaagaaa cagagaaatg
     1141 ttgaagtgcc cgacgaaaag tgctttgtag gtttcgatgc ctatgagaaa gtaatcgatt
     1201 caggtgtaga tatcgttctt ctctgcaccc ctccccactt ccggcctgca catgtcgaag
     1261 ccgcagtaaa tgccggcaaa cacatcttca tggaaaaacc cgtagctgtc gaccccgtag
     1321 gagcacgctc aatgatgatt tcagctgaaa aggcaaaaca gaaaggactg tgcatggtaa
     1381 gcggtacgat acgcagagtg cagaaagact ttatggagac gcagcgcagg gtcgccaacg
     1441 gcgaaatagg cgagatagta agcgcccata tcatacgaaa cggcggtgca ctatgggtaa
     1501 gaaaacgcca gcccggatgg accgacatgg aatatatgct ccgcaactgg gctaacttct
     1561 gctggctctc aggcgaccat atagtcgaac agttcattca cgaaatagac gtaatgaact
     1621 ggttcatagg taaatatcct gtaaaagcca tcggttgggg cggccggcag agaagagtaa
     1681 caggcgacca atacgatttc ttcagcattg aatatatcta cgacaacggc atgcatacac
     1741 actgcgccgc tcgacagata aatggctgca ccaatcttac aaaacagttc ttcgccggta
     1801 caaaagggta tgcaaatgca aaaggtgaaa tttataatct gaaaggagaa aaaatctgga
     1861 gctaccctta tcccgaaaaa gactcaccgg accagacgtg gaaagtgaac gacccattcg
     1921 tgcaggaaca tgttaatctt gtaacagcca tccgcacagg taatgtttac aacgatgctg
     1981 atgcccagat aaagtcaact atcacctgca taatgggacg catgtcggca tatactggta
     2041 aagacgttac gtgggaagaa gtcatgaact ccgaccttta cctcggtcca aaaacctatg
     2101 ccttcggacc tgtccccggc atcgaggaaa aaccaccggt gatcgggata gaaccgaaga
     2161 tttaagctta cggaacagac tgtttccctt aacctctccc ctgctttaag gagaagcaat
     2221 cgagggggaa aggccaactt ttaaataaaa gtcatggtat aattgaacga agaagcaact
     2281 ttaaacttaa acataatgaa ccgaagaacc ttcacaaaaa cagtagccgc agcaacggtg
     2341 gcaattctgg cattcaggct ttcatccttc tcaactaccc acccagcatc tgcaagaaac
     2401 cttaaaaaag gagttatgtg gggcagtatt gcttatgcca aaacggtgcg ggaaaaattc
     2461 cttgccgcaa aagaagccgg cttcgacggt gtagaggtta acagccacct cgacaggaaa
     2521 gaagtgctgg aggcaagccg cgaaacaggc ttacccgttg caagtgtctg cggcgctctg
     2581 cactggaaat acctactatc ggatcccgac ccgaaaataa gagaacaggg cattgaagca
     2641 ctcagagtaa ccatcgaaga cgcaaagaca tacggaactg atacagtgct tcttgtaccc
     2701 ggacgagtaa ccgaaacagt aagctatgac gaatgctgga acaggaccgt ggcagaaatc
     2761 aaaaaaattc tacctttcgc cgaaaagtca ggcgttacaa tagcaatcga aaacgtctgg
     2821 aacaacttcc tgctttcacc gctcgaagct gtaagatatg tcgaccagtt taaaagcaaa
     2881 cgggtaggtt tctatttcga tgtaggtaac atagtagcat acggctggcc ggaacagtgg
     2941 ataagaattc tgggcaaaag gatagcccgg gtacacatta aggaatacag ccgcaagatt
     3001 gcagattcac agggcagatc agcaggcttc aaggtaaacc tcatggaagg tgacgtaaac
     3061 tggccagcag tgatgaaagc tctcgatgag atcggctatt ccggctggac tacaattgaa
     3121 caaccaggcg gcaactcacc ggaaggtctg aaagacctgt gcgacagact ggtgaaaata
     3181 caggggagct gaacgtcact tcatcacgcc tgagacctgg taaaaaaatt taattcaggt
     3241 agcataaagc taaataactg acttacatat acttacggtt attgcttttt cactacgtat
     3301 ttcattgttt aatgccgggt taatagattt caatatatca cagatgaaaa ctcaaatttt
     3361 aattattttg attttttctc ttcttgcttc atgttctgaa gcaaggaaaa agacatccgg
     3421 tcacaggtat aacgactggt attacggaga aaacctttca cggattgcat tccctgttgg
     3481 aggaataggc gctggtatgt tctgcataga aggcaccgga gcaatatctc acatgtcggt
     3541 gcgcaatcgc ccggaagtgt tcaatgaacc atgcatgttt gccgcgctgt cagttgaagg
     3601 tatagaaaat ggtacaaaaa tacttgaagg tcctgttccc gactggaaaa aatttggacg
     3661 tcccggtacg ggcaatggcg cttcaggaac aagttacgga ctgccccgtt tccagaatgc
     3721 aaaatttatt tcccgctttc cctttgcaac aatagttctg caggatgacg atatacctct
     3781 cgatatcgaa cttacagcat ggagcccctt tatacccacc gatgcagata attcaagcct
     3841 tcccgcaggt gctgtcgaat acaaattcac aaacaccggc aataaatcca tacatgcagt
     3901 cttctcatac agttcgaaaa atttcatggc acaaaccggc ggaatgaact ccatcaaacc
     3961 gcttgccggg ggtttcatcc tttcagagga aggtataaaa gagagaccct ggacaaaagg
     4021 cgactttgca attttcactg acgacccggc cactgttgta gattactgct ggtttcgtgg
     4081 cgggtggtgg gacccactta ccatggcatg gaaaacaata agcagcactg aaaccctgag
     4141 caggaagcct gttgagaagg acgcacctgg cgcaacactt ttcgttcctt tcgatcttgc
     4201 gcctggagaa acaaagagtg tgaatctgat gttctcatgg tatgtacccg aatcaaacat
     4261 tcgttacgga agcgactcac aggaacccgg tgtaaccaga agtgaagatc cggctgctcc
     4321 tgagaaccag atgtattaca aaccatggta ctcaggccgt ttctcctcaa tagtggaagt
     4381 atcagattac tggaaagaaa actatgctgc tctaaaaaat aaatcggaac ttttcaggga
     4441 tgctttttac ggtcagactc ttccttcaga agttatcgaa gctgtagctg ccaatttgac
     4501 aatcctgaaa tcacccacgg tactccgtca gactgacggt aaactgtggt gctgggaagg
     4561 ctgcagcgac aactcgggat gctgtgcagg ctcatgcacc cacgtatgga actatgcgca
     4621 ggcaatacct caactcttcc cttctctgga aagaacgctg cgcgaaacgg agtttttcaa
     4681 aagccagaac agcgaagggc atcagacctt caggtcggca ctcccaatac ggcctgttgc
     4741 agcaacattc tacgccgcag ccgacggaca gctgggtggc attatgaaag tgtaccgcga
     4801 ctggcgtata agcggagaca acgactggct taaaatgata ttcccgaaag taattcagag
     4861 cattgattac tgcatccgca catgggatcc acgtcacacc ggaacactgg aggaacccca
     4921 tcataacaca tacgacatag aattctgggg acccgatggt atgtgtacca gcttctatct
     4981 tggcgcactc agggccgtat gtgaaatggg aaaatatctg ggagaaaaca tctccggata
     5041 tgaagagctc tatctcaaag gccgtaaatt tatggaagag gaactttttg acggggaata
     5101 ctttattcaa aaaattatgt gggaaggttt aaacgcaccc aatccggttg aagcttcaaa
     5161 aggtgcatgg aacaccgatt attccgatga agcaagggaa ctgctgcaga aggaaggtcc
     5221 taaataccag tacggcaaag ggtgtctgtc ggacggcgtg ctgggtgcat ggataggcga
     5281 agtctgtgga cttcataacg taattgattc cacaaaagtc agaagccatc tgaactcagt
     5341 tttcagatat aatttcagga gcgacctgag tgaccatgcc aatccacaac gtccctcgta
     5401 tgcacttggc aacgaaggag gactgctgct atgctcatgg cccagaggag gcgcactctc
     5461 actacccttc gtttacagta atgaagtatg gacaggaatt gaataccatg ttgcctctca
     5521 tcttatgatg atgggagaaa ctgaaaaagc actcaagata gtaagagcct gccgcgacag
     5581 atacgatggt cgtgtaagaa atccattcga cgaatatgaa tgcggccact ggtatgcccg
     5641 tgcaatgtcg agctacggtt tgttacaggg tttgacaggc gtcagatatg atgctgttac
     5701 aagaatactt tacctgaaag caggtatcgg cgacttcacg gttttcatct ccactgaaac
     5761 cggattcgga aaagccggaa ttaaaaaagg aaaacctttc tgtgaggtgg tgtacgggaa
     5821 aattgaaata aaagagtata aattaatcta aagagacagt ttgttgtttt atcttaatcc
     5881 gaaaatcacc ggaaatataa acacaactat cactgcccat atcgcataaa caggcaggta
     5941 tttcgcaatg gtgttttcca gtatcccggc actatctttc ttgaaattaa cacggtacag
     6001 gtctctggcg aagagtacca gattcccgag aatcagcaga tttgagccaa gtactgctgt
     6061 cctgtttggg gtaaagccat attctccaag tctgtaaatt attgccgaca gtgcaagcag
     6121 gtcggttata attgcaaaaa tgaccagcgc cagaagaatt acctcaagaa gccgctgcct
     6181 gaagaaaaag gttgtttccg aaactgaaaa aacaattatt gcagtcactc ccaacaacat
     6241 aacattgaaa accagcagaa aatccctgtt ggtatacaga ctctttccac tcaataatat
     6301 aaccagcagg tagataatca gtgtcagcag aaccagcgga ctgaagatac tggcaattac
     6361 aggcgggatt ttgcttgtaa tagctggaaa ataacggata agaaatgttg cggcaacagg
     6421 tgctgaaact gcaccaatta ttacaacata gtcagaatag aatcgctcgg cattgatatt
     6481 tatcacatcg aacaatccga ttgtcacagc tgttagtatc cctccggcaa ttgctataat
     6541 tccgaacata accgccagtt cgccgttgta tcttatgtag tttatccatc ctgatttttc
     6601 ccttaaattg aaaccagaat agaccaggcc gtaaataaac caaagaataa caggcagatg
     6661 cagacatgcc agcaaaacag tattcagatg aacatccccg ggaagaaggt tgatataaat
     6721 tgccgaaaca aagaatatca gagtggtaaa aatcagatgt actactttga aaccttttac
     6781 agtgagaagg taatagagtg tcagggctcc aattactacc agaccggcat tttttatata
     6841 gaaagcatcc atgtcagctt ctgtcctgaa aattgcggga aattttatga agaagcctgc
     6901 cagcgcacat gttaccagca gaaacaaaag atctttcttt acagtaataa gccaatcagc
     6961 ttttacagtg tcgggttcaa gccttgcttt ccagaatgct gaagcaggat gttctgatat
     7021 ttcaggatat atttccgaaa atgcctttgc aaagcctttt ctgtcgaacc ggtaaagttt
     7081 ttcgagctgt tccgggttct caatgttttc cttaatcaga ttcttcatta tcaataattt
     7141 ttatttcagg ttctgtcaaa tgactgttta ttaatatcac ctgctgcatc attttgcaaa
     7201 aaacgtagcc aatttactta attttacaga gctcctcgag taatgcatta atattatttt
     7261 ttgaatggaa tttttaaatt tcctgagaaa tttgacattt acttcaatcc ggtacatctt
     7321 tattaactag cttttgtaat atttcactcc tgctgagttc ttgtgccctg cgggtttctt
     7381 taaggctaca aacaaaacat ttgagaacag gcagtccgga gaatttttat aacaaaaaca
     7441 tttaaggaat tctcaaatca ttcttttgga ccggataagg gcttatccct attttaagct
     7501 tatccaattt caggtctgac agggccttct tacgtctgtt cacagcaaag aacatttacc
     7561 cttcataacg tcccgtcatt gtttctgcag aatcctgatt caatcgaatg ggaagttgct
     7621 ccttcagtct attttgatac accacgaagc ggtaagggag atactgttgt aaacataaat
     7681 ccatatcctg gttatatagg aaaacaggat gaaataaaat ttacccttca tcaaaacatt
     7741 aatgtggtga atatttcatg gcgtttccca atcctgcaaa cagctatgtg gatat
//
LOCUS       DAOSNB010000098         7651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9571_length_7651_cov_4.467220, whole genome shotgun sequence.
ACCESSION   DAOSNB010000098 DAOSNB010000000
VERSION     DAOSNB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9571_length_7651_cov_4.467220"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLG42_05415"
     CDS             <1..873
                     /locus_tag="PLG42_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOU30680.1"
                     /translation="LVMEFIKKHLDDYLHKNHETARILLKKIQDSEKERKAIASIQKL
                     ARERARKVSLHNKKLRDCRVHYNSNEPNKLDSTLFITEGDSASGSITKSRNVETQAVF
                     SLRGKPLNTYGLTKKIVYENEEFNLLQAALNIEDGIENLRYNNIVIATDADVDGMHIR
                     LLLLTFFIQFFPELIRKGHLYILQTPLFRVRNSKETRYCYTEEEKRQAIRDLGPNPEI
                     TRFKGLGEISPDEFRHFIGKDMRLEPVRMKRDDTVNGFLEFYMGKNTPERQDFIIENL
                     RVEEDIVEEELIRD"
     gene            926..3514
                     /locus_tag="PLG42_05420"
     CDS             926..3514
                     /locus_tag="PLG42_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOU30681.1"
                     /translation="MSQNPDQDSYSASLHSVASMSGMFRDWFLDYASYVILERAVPHL
                     LDGLKPVQRRILYSMRRMEDGRYHKVANIIGHTMQFHPHGDVSIGDALVQLGQKELLI
                     DTQGNWGNILTGDGAAAPRYIEARLSKFALEVLFNPKTTEWKLSYDGRNKEPVTLPVK
                     FPLLLALGTEGIAVGLASKILPHNFNELIDAAINYLMGKDFVIYPDFPTGGLIDVSKY
                     NDGQRGGMVRIRAKIEKLDKKTLVVREIPYGKTTSALIDSIIKASENGKIKIRKIDDN
                     TAANVEIVIHLMPGVSPDQAIDALYALTDCEYSVSPNSCVIIDNKPSFMGVSEMLRRS
                     VDHTVELLKAELSIRKEELLEEWHMMTLEKIFIEKKIYRDIENCETWESVLETIEKGL
                     APYKMNLLREITQEDIIKLTEIKIKRISKYDSAKAGEQIRKIEIELDEVKNHLKHIIP
                     YTINYFKQIKKKYGKGRERKTEIRNFDTIEAAKVVADNAKLYANFREGFIGTSLKKDE
                     FVCECSDIDDIIVIRRNGTYLVTKVAEKVFVGPEIIYAAVFHKNDDRTIYNIMYQDGR
                     DGKLLAKRCAITGITRDREYNLTKGKPGTKILYLSANPNGEAEIVRVYHRPRPRLKKP
                     VIEYDFSQLDIKGKNAIGNIVTKLPVQKVVLKEKGVSTLGGRKIWFDESVLRLNSDGR
                     GKLLGEFSGDDKILVITKSGSFRTTNFDLSNHFEDDLLLIEKFRPETVYSAIYWDADL
                     KFYYVKRFMVEETEKPLSFIGDNPESKLISLTEVEYPRFEVIFGGKHKGRKNEIVEVA
                     KFIGVKSYRAKGKRLTSYVVENIQEIEPVIKKETSLPQPDEEIGDEKKAIKDIRSDPA
                     QMKLEL"
     gene            3518..4045
                     /locus_tag="PLG42_05425"
     CDS             3518..4045
                     /locus_tag="PLG42_05425"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOU30682.1"
                     /translation="MPNRIYLTGFMGSGKTTAGKKLASAAGWDFIDLDHEIEKKEGKS
                     ISQIFALHGEDYFRNSEAETLRNLITLKDSVIATGGGTPCFHGNMNYMLATGIVVYLK
                     LKPEEIKKRLSAEQAERPLLIGISEEELLGFIRKKLGEREKYYNMAHIITDGYNFNEI
                     ILLEEIKKKNPGLLI"
     gene            4056..7121
                     /locus_tag="PLG42_05430"
     CDS             4056..7121
                     /locus_tag="PLG42_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30683.1"
                     /translation="MADLKKNPFPGLRAFREDEHNLFFGREGECREIIDKLLKNRFVA
                     VIGNAGSGKTSILQADVIPALKKGVTTAGNWRIILMTPGINPLGNLADSIAREVAPNK
                     SNYEALRISIADVLKSNQDGLNRIVNDLIIKKDEHVLIVVDQFEELFRYGKKALGPYT
                     GNGATAFVNLLMNAVEQQGKKIFLIVAISSEYIPECTRFYRFTQSIIINRSSVIISEI
                     PAEGLAPIIENPVKTSGAYIDPVLIQKITGEVSDRVIPVALVQHALTRTWDNWQIKGK
                     PQEPLNVSDFEKAGGLDKSVDIHGEEIFAELGTREKTVCELLFKTITGKGSGNERIRK
                     PSSAGLIKAVTGCSADELKAVLEAFACDKAGFIKYTGTSTVNDDSIIDVTYDCLLNSW
                     ARLKQWIDEEAESASTYLRLSEMATLYHEGKTGLLKPPELFTYLNWREKQKPSLQWAM
                     QYDPGYEKAISYLNSSETSYIEEEEKKKKKQKKKSIWRRLFILSLGTILITLAGITLV
                     MISRTESQRKATEAAELEKRKADSVALTLLNEKYRSDSVISLARVREQEIIARKEYAE
                     RQRIRTAIAASEALKKVERARSVADSLLRESLLAKQSEEEAIAQRNEAIRLRMVSTGK
                     SMAIRSLQIQEQKDLQILLAYQGYKFNKKFKGFPNDPDIYAGLYNIAKKYGNKYYKVL
                     SGHGGAVKSIALVPGKREFYTAGADGKVLRWNLENTTPAMRVIYSGSELIEVISLSPG
                     ADRLALGLDNSVIRVISLNGNKEPSFDLKGHAGKIQSLIFSYDGRYLYSAALDGRVLK
                     WDLALKTSVDLETGNVQITSIDISSGGNYLAGVSSDGKALVWNTELSTGVLRLENPGK
                     SIKIIRFKPGENIIAAGYSDGTLEMWNIEEKKKIHEIKAHEGEINQIRFNSVFSLLAT
                     ASSDKTLKIWDLSDVKALPLTFSDNNGVVMAVEFSNDGSLIITGIDASENNIIVRPST
                     ADLLAEDICQKLTRNFTEDEWYNYVGKDVGYEKTCSEKEFSIKVQQIRQ"
     gene            7198..7554
                     /locus_tag="PLG42_05435"
                     /pseudo
     CDS             7198..7554
                     /locus_tag="PLG42_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292015.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
BASE COUNT         2602 a         1426 c         1739 g         1884 t
ORIGIN      
        1 cttgtaatgg aatttatcaa gaagcatctt gatgattatc ttcacaaaaa tcatgaaact
       61 gccagaatac tcctgaagaa gattcaggat tcggaaaagg agaggaaagc catcgcatcc
      121 atccagaaac ttgcccgcga gagagcaaga aaagtcagcc tgcacaacaa gaagctgaga
      181 gactgcagag tgcactataa ttcgaatgag ccaaacaagc tcgactcaac cctctttatc
      241 actgagggcg actcggcaag cggctctata acaaaatcac gaaacgttga aacacaggct
      301 gtattcagcc tgagggggaa acctctcaac acttacggtt tgactaaaaa gatagtttat
      361 gaaaacgagg aattcaacct ccttcaggct gcattgaata tcgaggatgg aatagaaaac
      421 ctgaggtaca acaacatagt tattgcaacc gatgccgatg tcgacggaat gcatatcaga
      481 ctccttctgc tgacattctt tatccagttc ttccccgaac tgataaggaa aggtcatctc
      541 tacatccttc agactcccct tttcagggta aggaacagca aggaaacgag atattgctat
      601 acagaggagg aaaaaaggca ggcaatcagg gatctgggac caaatccaga aataacaagg
      661 tttaaaggtc ttggtgaaat atcacccgat gaattcaggc attttatagg taaagacatg
      721 cgccttgagc cggtacgtat gaaaagggat gataccgtaa acggatttct tgaattttac
      781 atggggaaga atactcctga acgtcaggac ttcattatag aaaatctgag agtcgaggaa
      841 gatatagtgg aagaagaatt aatcagggat taagatgaaa aaagaagagg aaaatctcgg
      901 ttttacagaa tgggaagatg gagggatgag tcaaaatcct gatcaggact catactctgc
      961 atctcttcat tctgttgctt ccatgtcggg aatgttccgc gactggttcc ttgattatgc
     1021 ctcatacgtc attcttgaaa gggcagtgcc acacctgctc gacggactga agccagtaca
     1081 gcgccgtatt ctttactcta tgaggaggat ggaagacgga cggtaccata aagtagcaaa
     1141 tataatcggt cataccatgc agtttcatcc ccacggcgat gtgtcaatag gcgatgcact
     1201 cgtgcagctg ggtcagaaag aactgctgat tgatacccag ggaaactggg gcaatatcct
     1261 cacaggcgat ggagcggcag caccccgtta tatcgaagcc cgtctttcaa agtttgccct
     1321 tgaggtactt tttaatccga aaactaccga gtggaaactc tcatacgacg gcagaaacaa
     1381 agaacctgta acactgcctg tgaagttccc gctgctgctt gctcttggta cagaaggaat
     1441 agccgttggt ctcgcctcta aaatactgcc gcataatttc aacgaactga tagatgcagc
     1501 tatcaactac ctgatgggta aagattttgt aatttatccc gacttcccaa ccggcggtct
     1561 gatagatgtt tcgaaatata acgacggcca gagaggaggt atggtaagga taagagcaaa
     1621 aatcgaaaaa ctcgataaaa agactctggt agtaagagag ataccctatg gaaaaaccac
     1681 ctctgctctc attgactcga ttatcaaagc aagcgaaaac ggaaagataa agatccgtaa
     1741 gattgatgat aacacggctg caaatgtgga gattgtcatc catctcatgc cgggtgtttc
     1801 gcccgaccag gcaatagatg cactttatgc tttgaccgac tgcgaatatt cagtttctcc
     1861 gaattcgtgt gtcataatcg acaacaagcc ttcttttatg ggtgtgagcg agatgctccg
     1921 ccgctcggtt gaccatactg tggaactcct gaaggccgaa ctcagcatca ggaaagaaga
     1981 actgctcgaa gagtggcaca tgatgacatt agaaaagatt tttatcgaga aaaagatata
     2041 ccgcgatatc gaaaactgtg agacatggga gtcggtgctg gagactattg aaaaagggct
     2101 tgctccgtat aaaatgaacc tgctgcgtga aataacgcag gaagatatca ttaagcttac
     2161 cgagataaaa atcaaaagaa tatcaaaata cgattctgca aaagccggtg aacagataag
     2221 aaaaattgaa attgaactcg acgaggtaaa aaatcacctt aaacacatca ttccctatac
     2281 aattaattat ttcaaacaga taaaaaagaa gtacggaaaa ggcagggaaa gaaagaccga
     2341 gataaggaat ttcgatacga ttgaagctgc aaaggttgtg gctgataatg caaagctcta
     2401 tgccaacttt cgcgaaggat ttattggcac ctcactgaag aaggatgaat ttgtgtgtga
     2461 atgctccgat attgatgata ttatcgtaat aaggagaaac ggaacctacc ttgttacaaa
     2521 agtggcagaa aaggtgtttg ttggtcctga aattatttat gcagcagttt ttcataaaaa
     2581 tgatgataga accatttata atataatgta tcaggatggc agagacggta aattgctggc
     2641 taaaagatgt gccataacag gtatcacgcg cgacagagaa tacaacctca caaaagggaa
     2701 gccaggtaca aaaattttgt acctgagcgc caaccccaac ggtgaggctg aaatagtgag
     2761 agtgtaccac agaccacggc cgaggcttaa aaagcccgta atagaatatg atttcagcca
     2821 gctcgacatc aaagggaaaa atgccatagg taatattgta accaaattac ccgttcagaa
     2881 agtcgttctg aaagaaaagg gtgtttctac actcggcggc agaaaaatat ggttcgacga
     2941 atctgttctc aggcttaatt ctgatggcag ggggaaactg ctgggtgaat tttcgggaga
     3001 cgataaaatt cttgtcataa caaaaagcgg ctctttcaga acaacaaact ttgacctgtc
     3061 aaatcatttt gaggacgacc tgcttctgat agaaaaattc agacccgaaa ccgtatattc
     3121 agcaatttac tgggatgccg atttgaaatt ctattatgta aaacgcttta tggttgagga
     3181 aactgaaaaa ccgctcagtt ttatcggtga taatcctgaa tcaaaactta taagccttac
     3241 tgaagttgaa tatccgcgtt ttgaagtaat attcggagga aagcataaag gacgtaaaaa
     3301 cgaaatagtt gaagtagcaa aattcatcgg agtaaaaagt tacagggcaa aaggtaaaag
     3361 actcaccagc tacgtggttg agaatatcca ggagatagaa cctgtgataa aaaaagaaac
     3421 atctttgcct cagcccgatg aagaaatagg tgatgaaaaa aaggcaatta aggatatacg
     3481 ttccgatcct gcccagatga agctagaact atgattgatg ccgaatagaa tatatcttac
     3541 aggtttcatg ggctccggca agacaaccgc cggcaaaaaa cttgcttctg cagcagggtg
     3601 ggatttcatc gacctcgacc atgaaataga aaaaaaagag gggaaatcga tatctcagat
     3661 ttttgcactg cacggggaag attatttcag aaatagtgag gcagaaacac tccgcaacct
     3721 tataactctg aaagattcag ttattgctac cggaggaggc actccttgct ttcacggaaa
     3781 tatgaattat atgctggcca caggaatagt tgtttacctg aaactgaaac ccgaagaaat
     3841 aaaaaagaga ctcagcgccg aacaggctga aagaccactt ttaataggaa tcagtgagga
     3901 ggaattactc gggtttataa gaaaaaaact gggggaaagg gaaaaatatt acaatatggc
     3961 acatattata accgatggtt ataattttaa tgaaataatt cttcttgagg aaattaaaaa
     4021 gaaaaatcct ggtttattaa tataattatt acgttatggc agacctgaaa aaaaatcctt
     4081 tccccggctt acgggcattc agggaagatg aacacaatct ttttttcgga agggaaggtg
     4141 aatgcaggga gattattgat aaacttctga aaaacaggtt tgttgctgtt atcgggaatg
     4201 caggctcagg aaaaacatcc atcctgcagg ctgatgttat acctgccctg aaaaagggag
     4261 tcaccaccgc aggaaactgg agaatcatat taatgactcc aggaattaat cccctcggta
     4321 accttgccga ttcaattgca agggaagtcg ccccgaacaa aagtaattat gaggcactca
     4381 ggattagtat tgctgatgtg cttaagtcaa accaggacgg attaaacaga atagtgaatg
     4441 acctgatcat taaaaaagat gagcatgtat taatcgtggt cgaccagttt gaagagttgt
     4501 tccgttacgg taaaaaagca cttggaccct ataccggaaa cggagcaact gctttcgtta
     4561 accttctgat gaatgctgtt gaacaacagg gcaaaaaaat atttcttata gttgcaatat
     4621 catcggaata tattcctgaa tgtaccagat tttatcgatt tacacagtca ataataataa
     4681 acagaagcag tgttattatt tcagaaattc ctgcagaagg gctggctcca ataattgaga
     4741 atccagtcaa aacttccggc gcttatattg atcctgtgct aatccagaaa attaccgggg
     4801 aagtttccga cagggttatt ccggtagccc tggtccagca cgcactgacg agaacgtggg
     4861 acaactggca gataaagggg aagccgcaag agccgctgaa cgtttccgac tttgaaaaag
     4921 ctggcggact tgataaatct gttgatatac atggagaaga gatttttgca gaactgggca
     4981 ccagggagaa aactgtttgt gaactgcttt tcaaaacaat tactggaaaa ggttcaggta
     5041 atgaaagaat aagaaaacct tcttcggcag gactgataaa ggccgtaacg ggttgttccg
     5101 cggatgaact gaaagcagta ctagaagcat ttgcctgcga taaagcagga tttattaaat
     5161 acacaggcac atcgaccgtt aatgatgatt ctataataga tgtaacttac gactgccttc
     5221 taaattcatg ggcaaggctc aaacaatgga tcgatgaaga agcggagtcg gcttctacat
     5281 accttcgcct gtcggaaatg gcaacactat atcatgaagg gaaaacaggg ctgttaaaac
     5341 cacctgaact ttttacatat cttaactggc gcgaaaaaca aaaaccatca ctgcaatggg
     5401 ctatgcagta cgaccctggc tacgaaaaag ccatctctta tctgaatagc agcgaaacaa
     5461 gttatattga ggaagaagaa aagaagaaga agaaacagaa gaaaaaatca atatggagaa
     5521 gactcttcat cctttccctg ggaacaatac ttattactct tgcaggaata actcttgtta
     5581 tgatttcacg gactgagtct cagcgaaaag ccactgaagc tgcagaattg gaaaaaagga
     5641 aagcagattc agtagcatta actcttctga atgagaaata ccgttccgat tctgttattt
     5701 cccttgcaag agtcagagaa caggaaataa ttgcccggaa agaatatgcc gaacgtcaaa
     5761 gaatacgtac agctattgct gcaagtgaag ctctcaaaaa agtagaaaga gcaaggtctg
     5821 tggcagattc tttattaagg gaaagcctgc tagcaaaaca aagtgaggaa gaggcaattg
     5881 ctcaaagaaa tgaagcaatc cggctcagaa tggtttcaac aggtaagtca atggctatca
     5941 ggtcgttaca aatacaggaa caaaaagacc ttcagatact tcttgcttac cagggatata
     6001 aattcaataa aaaatttaag ggtttcccta atgatcctga tatatatgcc ggactatata
     6061 acatagcaaa aaagtacggt aacaaatact acaaagttct cagcggacat ggtggagcag
     6121 tgaaaagtat tgctttagtt ccgggtaaaa gagagtttta tacagcaggg gcagatggaa
     6181 aggttctcag atggaacctt gaaaatacta ctcctgctat gcgggtaatt tattcaggca
     6241 gtgaattaat tgaagtaata tcactcagtc ccggtgccga ccgtctggca ctaggtctcg
     6301 acaactcggt aataagagta atatctttga atgggaataa ggagccctct tttgatctga
     6361 aaggacatgc cggaaagata caatctctta ttttttcgta tgatgggcga tacctttatt
     6421 ctgcagctct cgacggaaga gtcctgaaat gggatctggc acttaaaaca tcagtagatt
     6481 tggaaacagg gaatgttcag attacttcta tagatatttc atctggcggt aattatcttg
     6541 caggtgtttc ctccgatggc aaagcactcg tctggaacac tgaattaagt acaggggtcc
     6601 tgagattaga aaatcctggc aaaagcataa agattatcag attcaaaccc ggggaaaata
     6661 taattgctgc aggatattcc gacggaactc ttgaaatgtg gaacatcgaa gaaaagaaaa
     6721 agatacacga gattaaagcg cacgaagggg aaattaacca gattcggttt aatagcgtat
     6781 tttcgctgct ggcaactgct tcaagtgata aaacattgaa aatatgggat ttatcagatg
     6841 ttaaagcact gcctttgact ttcagcgata ataatggcgt tgtgatggca gttgaattca
     6901 gtaacgatgg cagcctgata atcacaggga ttgatgcctc agagaacaat ataatagtga
     6961 ggccatcaac tgctgacctg ttggctgagg atatttgcca gaaacttacc cgtaacttta
     7021 ctgaagatga atggtataat tatgtcggga aagatgtcgg atatgaaaag acctgttcgg
     7081 aaaaggaatt cagcatcaag gttcaacaaa taagacagta aatttgtttt aatttgtggg
     7141 taaggtaaaa cattatgaat tttacctatt tttgccagtt cattaaacta aaacaaaatg
     7201 acaggaaaag ataaaaaatt tctactgatg atccttgatg gatggggcgt tggcgatggt
     7261 tcaaaagctg atgtgattag tagtgcacca actcccaatc tcgataatct caaaagcaga
     7321 tatccgaatt cgatacttca tgcatcgggt gaaaatgtgg gattacctga aggtcagatg
     7381 ggtaactcag aagtagggca tcttaatatc ggagccggaa gaatcattta ccaggatctg
     7441 gtaaaaataa acgttgaatg taagacagga gaaataagaa aaaataaggt gctcgttgat
     7501 gccttttcgt atgccagaga caataataaa caggttcact tttttggtcg aacacgtcgt
     7561 agtgaattcc atattgggac aatttgtcga gcaggcgcgg tatgttgatg cgcccgtccg
     7621 gcaattccag aatacgcctg gcatgaactg c
//
LOCUS       DAOSNB010000099         7607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9645_length_7607_cov_4.550318, whole genome shotgun sequence.
ACCESSION   DAOSNB010000099 DAOSNB010000000
VERSION     DAOSNB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9645_length_7607_cov_4.550318"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLG42_05440"
     CDS             complement(<1..202)
                     /locus_tag="PLG42_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30684.1"
                     /translation="MSSKAVRILVVMLLFTTFARSQNSNVMYYMNIPQNHLLNPALRP
                     SNTFYLGLGITGIGIGLNNNYFN"
     gene            complement(215..2338)
                     /locus_tag="PLG42_05445"
     CDS             complement(215..2338)
                     /locus_tag="PLG42_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30685.1"
                     /translation="MKGKIFNTIIILTVLYLSGCIKETYDLNKLSEEVQISPVFAFPV
                     ATGDLTFADILKPNDTVRYESDNFVKIVVRKDSVVFLKLEDYYDFSNLVSYKKGFELG
                     EVEINDFQTSTTLKLNDIIQYFSPTLRQQFINLDDGQPHDFPSFPVTDLGEHSFPVIV
                     SFENVLFGSGILQIGVTNNLTAPLKDIKIRLYTNPGHIQVGTEKTITGPIYPGATKTA
                     EIDLSGAAVKNSFIAAIILTGSDGASNVIIDLDHTVVFSVNGYNLKIKAGRAIIKDQI
                     LDLDDDADTVDVDLGEDIEIERAKIKKGTLQYKITSKSNVTGSVSFALPTSSRNNNGQ
                     LTEMISVPPQQTTTGSVDLSNISIDLSTDPGQPYNRLPVTNQVKIGSNNIIIDYSMYD
                     SIYVTLDVSDAEVDFVKGYFGQREEQIKPDTIDTELEDILEKISGEFHISKPSVKVNY
                     SNSFGIPVEVTLNAKGKRDNKIQNLNLAPFVIINPVDTVVRDVSSVFTIDGTNSSLPD
                     LISLPPVEAVFTGSAKMNPAGSAGGRNNYVFGNSRLLASLEIEVPLELWINNLQFADT
                     VDNFLKSEENEEEKDSPFRPENMEYFRIKITTSNGFPLGVSIELMLYDTVTGRVLDTI
                     SAPGLIKPALVDASGKVVSPVETVTEIEFDKRFFEASKDADKIIFIFKLVTSGGGTKD
                     VKFYSDYKISYKATINAKPKFIFNK"
     gene            complement(2351..5044)
                     /locus_tag="PLG42_05450"
     CDS             complement(2351..5044)
                     /locus_tag="PLG42_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30686.1"
                     /translation="MAKRLILLLIILFSGLAALFLSIQRGSRNILTDPYKAIPADVSV
                     LIETVNFPGLLNSVSENNSLFKEITGIKEFEKFGEKFNFLRAIINNSEITKTIENHKT
                     LVSFHKVDSQNLVPLIVITVPKFVRFSHIREYLTPFKDLNINVLRKGKDRFLEIVREN
                     PEREKFYLSFVSGLVLCSKSVALIEEAILRSKSGSDLRDDTGLSRLLASVGKNEDRIF
                     FIFNNLKDAIKEIIKSPGIAGKITNLAQGAEGVIIINDNGYLFSGYADTNDSSDILFS
                     YKLLEPAQLNIFRQLPANTVLFETKVISGKSAGMRGEMGKSNQLLDELQPYLGGETTR
                     AIVDLRRTGITTKATLLVYSLKNRDIAERIISDRFTIWCNENNLDENKYIRYFQPDEQ
                     TRIPVLSTPFKGIGASFFGECMVKVADSLMAFYDNYLVMSDRKEAIEMFIYDNLLHKT
                     ISTNPDFSELESTLPSKACYFFYCVPGAVIENLSEFFNDRVNKILSENSTLLKKIRCV
                     GYQFVPSNDMIFNTLSVRYVESVESEPGGTEWESKLDGAVVTKPLFFTNHNTGAKEIV
                     LQDDMNNLYLINAAGRILWKIQPGERVNGNIYIIDFYRNGKFQILFAGVNNLYIFDRN
                     GNRVERFPVKLRAPASGPPALFDYDNNRDYRIFIPGEDKIIYAYDKYGNTVKGWKLFK
                     TNGFVRSEIKFFRLSGKDFIVAADENTVYFLDRTGNIRLKPSQPVICARGSQIRSDSS
                     GEPSFVFSAPDGTVQFIYLDGRVKKVILNKFSSEHFFDFFDIDGDGFGEFIFIDGSKL
                     FLYDHDMTEIFIRDFGTINIKGPYNFVFSSADRKIGIFDPVKKQVFLIDKKGNDVKGF
                     PVRGCSEFSIGNLSKGGDYNLIVGGNDNFLYNYKLNTLGNY"
     gene            5203..>7607
                     /locus_tag="PLG42_05455"
     CDS             5203..>7607
                     /locus_tag="PLG42_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU30687.1"
                     /translation="MAKVLKALFIIGSFTVAIAGAQEITIVDADDRKPLPDVAIMLED
                     GKFLTYTSQSGIVDISQFNDNQTICFHLVSFERLCVTAGEIRNSGNIIELKKRVFEIE
                     EFVVSASRREQRKSETPGRIAVVTDRLIKFQNPQTAADLIASSDEVFVQKSQLGGGSP
                     MIRGFATNRVLIVVDGVRMNNAIYREGNIQNVISLDPNVIESAEIIFGPGSIVYGSDA
                     IGGVMDFHTDKVMLSSFNKPLFKAGAMIRYSSANNEKSSQIKINGGFKKAGFYGAISY
                     SSFDDLRMGSHKHPEYIRPEYAMYINKKDTIIKNSNPDVQIPSGYSQLNTTGKFRYRF
                     SNNLDVVFSNHFSALSNVPRYDRLIQYKSGTLRYGEWYYGPQVWMMNNLQVSYSSPNI
                     LFNHLKLTAAYQNYRESRHDRAFGKYNINEQFEKVKIFSLNLDFDRKSSSGKSLLYYG
                     LELVTNNIKSEAWLRNILTVETTPAGSRYPNGKNRYNSYSAYAGYRINLSDRTIFSSG
                     LRYNYVTLNSTIADNSFYNFPFTRIDISNGALTGSAGVVIRPGQKTCLSINASTGFRA
                     PNLDDAGKVFDSAPGIVVVPNPDLKPEYAYNIDAEIEKDFGKFLHFEFSGFFTWLNNA
                     MVRHDFLFNGEDSIMYQGQLSKVEAIVNASYARVYGFSANVYLNIMRHFYLKSVLNVT
                     EGREKGNIPLRHSAPLFGSTHLIFENQSLKADLYSVYNGPKKFSDMPPSEREKPYIYA
                     TDINGNPWSPGWFTINFKISYTIPGIVLLSAGCENILDYRYRPYSWGIVAPGRNFILS
                     LRVT"
BASE COUNT         2330 a         1515 c         1357 g         2405 t
ORIGIN      
        1 aattaaagta attattgttt aagcctattc caataccagt tattccgagt ccgagataaa
       61 atgtgtttga aggtctgagt gcaggattca gcaaatgatt ctgtggaatg ttcatataat
      121 acattacatt gctattctgc gacctggcaa atgtcgtgaa aaggagcatt acaaccagta
      181 ttctgacagc ttttgaggac atgtttgaat atttttattt attaaagata aacttcggtt
      241 tggcattaat ggttgcttta tatgaaatct tataatcaga ataaaacttt acatctttag
      301 taccgccccc ggaggtgaca agtttaaaga taaaaataat tttgtctgca tcttttgaag
      361 cctcgaaaaa acgcttgtca aattcaattt cagtaactgt ttcaacaggt gaaacaactt
      421 tccctgaagc atcaacaaga gctggcttta tcagtccggg ggcactgatt gtgtcgagca
      481 ccctccctgt gaccgtatca taaagcatca gctcaattga taccccaagc ggaaatccat
      541 ttgaagtggt tattttgatt ctgaagtatt ccatattttc cggccggaaa ggactatcct
      601 tctcttcttc gttttcttct gatttcagaa aattatcaac tgtatctgca aactgcagat
      661 tgttaatcca taattcaaga ggaacttcaa tttcaagtga agcaagcagc ctgctgttac
      721 caaatacata attatttcgc ccgccagccg atcccgcagg gttcattttt gcagaaccag
      781 taaataccgc ctccacaggt ggcaatgata tcagatcggg caaactggaa tttgttccgt
      841 cgatggtaaa gacagaagaa acatccctga ctactgtatc aaccggatta ataattacaa
      901 atggagccag attcagattc tgtattttat tatccctttt cccttttgca ttcagtgtaa
      961 cttctacagg aatgccaaat gaatttgaat agttaacctt aacagatggc ttcgaaatat
     1021 gaaattctcc acttattttt tcaagtatat cttccagttc tgtatctatt gtatcaggct
     1081 taatctgttc ctcacgctgc ccgaaatatc ctttcacaaa atctacttca gcatccgata
     1141 catccagtgt aacatatatg ctgtcgtaca tactgtaatc aataattata ttattggagc
     1201 cgattttaac ctgatttgtg accggcaatc tgttgtaggg ctgcccggga tcagtgctaa
     1261 gatctattga aatattgctc aggtcaacag accctgttgt tgtttgctgt ggaggaacag
     1321 aaatcatttc agtgagctgc ccgttattat tacgcgatga agttggcaga gcaaaagaaa
     1381 ctgatccggt cacattactc tttgaagtta ttttgtattg cagagtccct ttcttgattt
     1441 ttgcccgttc tatttcaata tcttcaccaa ggtcaacatc aacagtatct gcatcatcat
     1501 ctaaatcaag aatttgatcc ttgatgattg ccctgcctgc tttaattttc aggttgtaac
     1561 catttacaga aaaaacgact gtatggtcga gatcaataat tacatttgaa gcaccatcgc
     1621 tccctgttaa aataattgct gcaataaatg aatttttaac tgctgcaccg ctaaggtcaa
     1681 tttcagcggt ttttgttgct cccggatata tcggacctgt aatggttttt tccgtaccca
     1741 cctggatatg ccccggattg gtgtacaagc ggattttaat atcttttaga ggtgctgtaa
     1801 gattatttgt aacaccaatc tgaagtatac ctgaaccaaa tagaacgttc tcaaaactga
     1861 ctatgacagg gaaagaatgc tcccctaaat ctgtaacggg aaaagacgga aagtcatgag
     1921 gctgcccatc gtcaagatta ataaactgct gccttagggt tggactgaaa tactgaataa
     1981 tatcattaag ttttaatgta gttgaggtct ggaaatcatt tatctcaacc tcacccagtt
     2041 caaatccctt cttatatgaa accagatttg aaaaatcata atagtcttcc agcttcagga
     2101 aaaccaccga atccttccta accacaatct ttacaaaatt atcactctca tatctgactg
     2161 tatcatttgg ttttaatatg tctgcaaatg tcaggtcacc agttgctacc ggaaatgcaa
     2221 atactggaga aatctgcact tcttccgaca atttgttcag gtcgtatgtc tcctttatac
     2281 atcctgataa gtataagacc gtcaggataa taatcgtatt aaatattttc cctttcataa
     2341 atcataatca tcaatagttt cccaatgtat ttaacttata attataaaga aaattgtcgt
     2401 tcccgcccac aataagatta taatcacccc cttttgacaa attgcctatt gaaaattccg
     2461 agcatcctct tacaggaaaa cccttaacat catttccttt tttgtctatc agaaaaacct
     2521 gtttcttaac cggatcaaaa atacctattt tcctgtcagc tgaagaaaac acaaaattat
     2581 acggaccttt aatgtttatt gttccgaaat cacgaataaa tatttccgtc atatcatgat
     2641 catatagaaa taatttactt ccgtcaatga aaatgaattc accaaatccg tcaccatcta
     2701 tatcaaaaaa gtcaaagaaa tgttcggaac tgaatttgtt cagtataact tttttcactc
     2761 ttccgtcgag gtaaataaac tgtactgttc catcgggagc tgaaaatacg aatgatggtt
     2821 cacctgaact atctgatctg atctgtgaac ctctggcaca tatcacaggc tgggaaggtt
     2881 taagtctgat atttcctgtt ctatcaagga aatatactgt gttctcatca gctgcaacaa
     2941 taaaatcctt tcccgaaagt ctgaaaaact ttatttctga cctaacaaaa ccgtttgttt
     3001 tgaatagttt ccatcctttt acggtatttc cgtatttatc atatgcataa ataattttgt
     3061 cttctcccgg tataaatatt ctgtaatcac ggttattgtc ataatcaaaa agtgcagggg
     3121 gacctgatgc cggagcacgc agcttgaccg gaaacctttc aacacgattc ccgtttctgt
     3181 cgaaaatata aagattattt actccggcaa agagaatctg aaacttgcca ttcctgtaaa
     3241 aatcaattat gtaaatgttt ccgttgacac gttcccctgg ttgtattttc cacaatattc
     3301 tccctgcagc atttataaga taaaggttgt tcatgtcgtc ctgcaacact atttccttcg
     3361 cacctgtgtt atgatttgta aagaacaaag gttttgttac tactgctccg tcaagcttgc
     3421 tctcccattc tgttccacct ggttcactct caacactttc aacatatctg acagacaggg
     3481 tattgaaaat catatcgtta ctcggcacaa actgataacc aacacatctt attttcttca
     3541 gaagtgtgct attctcactc agaatcttat ttaccctgtc attgaaaaat tctgaaagat
     3601 tttctataac agcacctgga acgcagtaaa aaaagtagca ggctttactc ggaagggttg
     3661 attcgagttc actgaaatcg ggattcgttg aaattgtctt atgcagaaga ttatcataaa
     3721 taaacatttc aatggcttcc tttctgtcag acatcacaag gtaattatca taaaatgcca
     3781 tcagagaatc agccactttt accatgcatt ctccaaaaaa ggatgcacca atgcctttaa
     3841 aaggggtgga gagaaccgga atcctggtct gttcatccgg ctgaaaatac cttatatatt
     3901 tattctcatc cagattattc tcattacacc atattgtaaa tctgtcactt ataatccttt
     3961 ctgctatgtc tctgtttttc agactgtaaa ccagtaatgt tgcttttgtg gtgattcctg
     4021 tccttcttaa atcgactatg gcccttgttg tttctccgcc aagataaggt tgaagttcat
     4081 ccaatagttg attgctctta cccatttctc ctctcattcc ggcacttttt cctgaaatca
     4141 cttttgtctc aaataaaact gtattggcag gaagttgcct gaatatgttt agttgcgctg
     4201 gttcaagtaa cttataactg aagagaatgt cagaactgtc attagtgtca gcataacctg
     4261 agaacaggta tccattgtca ttaataatta tcaccccttc agctccttgt gccaggttgg
     4321 ttattttccc ggctataccg ggtgatttaa ttatctcttt aatggcatct ttcagattgt
     4381 taaatataaa gaatattctg tcctcatttt ttccgaccga tgcaagcagc ctggaaagac
     4441 ccgtgtcatc tcttaaatct gacccggatt tacttcgcag gatagcctcc tctatcagtg
     4501 caaccgattt actgcaaagt acaagacctg aaacgaaact caaataaaat ttttctcttt
     4561 caggattttc tctgacaatt tccaggaatc tgtctttccc ctttcttaat acattaatgt
     4621 ttaaatcctt gaagggtgtc agatattccc tgatatgaga gaaccttaca aatttcggaa
     4681 cagtaattac tatcagggga accagattct gactgtcaac tttatgaaaa gagactaagg
     4741 ttttatggtt ttctatggtt tttgtaatct cactgttatt aattatagcc cgtagaaaat
     4801 tgaatttttc tccgaacttc tcaaactctt ttattcctgt tatttcctta aacagactgt
     4861 tattctcaga gactgaattt aataagccgg gaaaatttac agtttcaatt aaaactgaaa
     4921 catcagccgg tatagccttg tagggatctg tgagtatatt acggcttccg cgctgtatgg
     4981 acaagaataa agcagccagg cctgaaaaaa gaataatcag cagcagaatt aatcttttag
     5041 ccacaatttt tacccttctt taatttacaa tctcaaaaat atcaaatata tttagtttat
     5101 aataaaaaaa ctgacattca acttataaca aacatccgtt acgaaatggc ttaaaactat
     5161 taatatcttt gcaggtactt ttgattaacc ttaatttcat tgatggcaaa ggttttaaaa
     5221 gctttattta taataggttc attcaccgtt gcaattgcag gagctcagga aattacaatt
     5281 gttgacgctg atgacagaaa gcctctgcct gatgttgcaa taatgcttga agatgggaaa
     5341 tttctaacat acaccagcca gtcaggaatt gttgacattt ctcaatttaa tgacaatcag
     5401 acaatttgtt ttcatctcgt ttcatttgaa agattatgtg ttactgccgg tgaaattaga
     5461 aactcaggga atattataga attgaagaaa agagtttttg aaatagaaga atttgttgtt
     5521 tcagcaagtc ggagggagca aagaaagagt gaaacaccgg gcaggatagc tgttgtaacc
     5581 gaccggttga taaagtttca gaatcctcag acagctgcag atctgatagc ttcatcagat
     5641 gaagtttttg ttcagaaaag ccagctgggc ggaggcagcc cgatgatcag gggctttgca
     5701 acaaaccgtg ttctcatagt ggttgacggg gtcagaatga ataatgctat ctacagggaa
     5761 ggcaatattc agaatgtcat ttcgctcgat cccaatgtaa ttgaatcagc agagattata
     5821 tttgggcccg gttccattgt atatggaagt gacgcgatag gtggagtaat ggatttccac
     5881 accgataagg ttatgctctc atcttttaat aagcctcttt ttaaagctgg tgcaatgata
     5941 aggtactctt cagccaataa tgagaaaagt tcacaaataa aaattaatgg gggattcaaa
     6001 aaggctggtt tttatggcgc catatcatac tcaagtttcg atgacctcag gatgggttcg
     6061 cataaacacc ctgaatatat aaggcctgaa tatgccatgt acattaacaa aaaggatact
     6121 atcattaaaa actctaatcc cgatgttcag attccttcgg gttacagcca gctgaataca
     6181 accggcaaat tcagatacag attttccaac aaccttgatg ttgttttctc aaatcatttt
     6241 tcagcccttt ctaatgtgcc ccgatatgac agacttattc agtacaaatc gggcactctc
     6301 aggtatgggg agtggtatta tggaccccag gtatggatga tgaataatct tcaggtaagt
     6361 tattccagtc caaatatttt atttaaccat ctgaaactta cagcagctta tcagaactac
     6421 cgcgaaagtc gccacgacag agcattcggg aaatataaca taaatgagca gttcgaaaag
     6481 gttaagatat tttctctcaa cctcgatttt gacagaaaga gttcctcagg aaaaagcttg
     6541 ttatattacg ggttggagct tgttaccaac aacataaaat ctgaggcatg gttaagaaat
     6601 attttaactg ttgaaacgac cccggcaggg tccaggtatc cgaatggaaa gaacagatac
     6661 aacagttatt ctgcatatgc aggatacaga ataaatttat cagaccgcac aatattcagc
     6721 tcaggattga ggtataatta tgttacactg aattcaacta tcgctgataa ttcattctat
     6781 aattttccat ttaccagaat tgatatttca aacggtgcac ttacaggctc agccggtgtt
     6841 gtgatcagac cgggtcaaaa aacctgcctt agcattaatg cttcaacagg attcagagca
     6901 cctaatcttg atgacgctgg aaaagtattt gattctgcac ccggaatagt ggttgtacct
     6961 aatccggatt taaaaccaga atatgcttac aatattgacg cagaaattga aaaagacttc
     7021 ggaaaatttt tgcatttcga attctcgggc tttttcacat ggctgaataa tgcaatggtc
     7081 aggcacgatt ttcttttcaa cggtgaagac tcaattatgt atcaggggca attaagcaag
     7141 gtagaagcaa ttgtaaatgc cagttatgcc agagtttatg gtttttcggc aaacgtctat
     7201 ttaaatatta tgagacattt ttatctcaag tcagtgctga atgttacaga aggaagagaa
     7261 aaagggaata tccccttgag acactctgcc ccattatttg gttcaacgca tcttatattt
     7321 gagaatcagt cattaaaggc agacttgtac tcggtgtaca atggtccaaa aaagttttct
     7381 gacatgccgc cctcagaaag agagaaaccc tatatttatg cgaccgatat aaacggaaat
     7441 ccatggtcgc ctggttggtt tacaataaac tttaaaatca gttatacaat ccctggcatt
     7501 gttttactta gcgccggatg tgaaaatatt ctcgattacc gttaccggcc atattcatgg
     7561 ggtatagttg ccccggggag aaatttcatt ctgtctctca gagtgac
//
LOCUS       DAOSNB010000100         7603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9654_length_7603_cov_3.904610, whole genome shotgun sequence.
ACCESSION   DAOSNB010000100 DAOSNB010000000
VERSION     DAOSNB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9654_length_7603_cov_3.904610"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PLG42_05460"
     CDS             complement(<1..1201)
                     /locus_tag="PLG42_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU30688.1"
                     /translation="MSFRKDTKVKTSYSRISIALASPEEILDRSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIT
                     LVVPVAHIWYFRSIPNKIGYLLGLPSKKLDSIIYYERYVVVNPGIKAADGIKYLDFLT
                     EEEYLEIMESLPKENQLLDDSHPDKFIADMGGEALYKLLSRLDLDELSYSLRHKASIE
                     TSQQRKSEALKRLQVVEAFRESKHVNRPEWMIIKVLPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKVNAVKTEANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVAGPELKLHECGLPKDMAAELYKPF
                     IIRKLIER"
     gene            complement(1244..5053)
                     /gene="rpoB"
                     /locus_tag="PLG42_05465"
     CDS             complement(1244..5053)
                     /gene="rpoB"
                     /locus_tag="PLG42_05465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOU30689.1"
                     /translation="MSSKVTERISFASRKEQPGYPDFLEVQIKSFREFFQLGTTPENR
                     KKEGLYKVFSENFPITDTRNNFVLEFLDYSIDPPRYTVEECIERGLTYSVPLKAKLKL
                     YCTDPEHEDFDTVIQDVYLGTIPYMTDRGTFIINGAERVVVSQLHRSPGVFFGQSIHP
                     NGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFETDKQILEI
                     FNLAEEHKVSKASLKKFIGRKLAARVLRTWIEDFVDEDTGEVVSIERNEIILDRETII
                     EPEHVDLIVESGAKTILLHREDVSLSDYAIIYNTLQKDPCNSEKEAVLYIYRQLRNAE
                     PPDEATARDVIEKLFFSDKRYDLGDVGRYRINKKLGLDIDPNIKVLTKEDIIKIIIYL
                     IELINSKTEVDDIDHLSNRRVRTVGEQLSAQFSVGLARMARTIRERMNVRDNEVFTPI
                     DLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRVSALGPGGLSRERAGFEVR
                     DVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIESPYRRVVNGKVDFSDEGI
                     VWLNAEEEEGKRIAQANAPLNEDGSFKEQRVKCRMGADYTNEEPSNVDFIDIAPNQIA
                     SIAASLIPFLEHDDANRALMGSNMMRQAVPLIRPETPIVGTGLEKQVIQDSRILIVAE
                     GDGVVEYVDANEIVIRYTRTDEEKFVSFDEDVKHYYLPKYRKTNQNTCINLTPIVRKG
                     EKVKKGQVLTEGFGTKDGELALGRNLMVAFMPWKGYNFEDAIVISERVVQEDLFTSIH
                     IDEYLLEVRDTKRGLEELTSDIPNVSEEATKDLDENGIIRIGAHVRPGDILIGKITPK
                     GESDPSPEEKLLRAIFGDKAGDVKDASLKAGPSLEGVVIDKKLFTRAIKDRKSKNVEK
                     PMLEKLDAEFNKNVEELKSRLVDKLFILVNGKTSQGVKDYLNVDIIPKGAKFTLKQLQ
                     EIDYLNINPNKWTTDKKKNDSIRKLIHNYIIKYKEIDGVYKRKKYNITIGDELPAGIV
                     QLAKVYIAKKRKIKVGDKLAGRHGNKGIVAKIVRVEDMPFLEDGTPVDIVLNPLGVPS
                     RMNLGQIYETVLSWAGQKLGLKFSSPIFDGATLDQINEYVEKAGLPKNGTTYLYDGGT
                     GERFDQPATVGVIYMMKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEV
                     WALEAFGAAHILQEILTIKSDDVIGRAKAYEAIVKGEPMPVPGIPESLNVLLHELRGL
                     GLSVMLD"
     gene            complement(5152..5538)
                     /gene="rplL"
                     /locus_tag="PLG42_05470"
     CDS             complement(5152..5538)
                     /gene="rplL"
                     /locus_tag="PLG42_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOU30690.1"
                     /translation="MADLKKLAEELVNLSVKDVNELAKILKEEYGIEPAAAVAAVAAP
                     AAAGAGPAAPAAEEKTSFDVILKSAGGQKLQVVKLVKDLTGLGLKEAKELVDKAPGPI
                     KEGVTKEEAENIKNKLTEAGAEVEIK"
     gene            complement(5574..6092)
                     /gene="rplJ"
                     /locus_tag="PLG42_05475"
     CDS             complement(5574..6092)
                     /gene="rplJ"
                     /locus_tag="PLG42_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOU30691.1"
                     /translation="MKREEKNAIVESLANYIREYNHFYLTDIAHLNAADTSALRRKCF
                     ENQIKLVVVKNTLLKRALEQVNGNFEELFPVLKGATSVMFAKSATIPARVIKEFRKQH
                     DKPVLKGAYVQESVYIGDKMLDVLVSIKSKEELLGDIILLLQSPARNVISALQSGSTK
                     LHGVLETLSKKE"
     gene            complement(6116..6811)
                     /gene="rplA"
                     /locus_tag="PLG42_05480"
     CDS             complement(6116..6811)
                     /gene="rplA"
                     /locus_tag="PLG42_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOU30692.1"
                     /translation="MAKLTKNQKFALEKVDRTKMYTLSEAAKLVKEITRTKFDASVDI
                     DIRLGIDPRKSNQMVRGVVSLPHGTGKQTRVLVLCTPDKEAEAREAGADYVGLDDYIE
                     KIKGGWTDVDVIITMPSVMGKIGQLGKILGPRGLMPNPKNGTVTMDIGKAVKEVKQGK
                     IDFKVDKNGIIHASIGKVSFEPSKIVDNAREFISMVNKLKPPAAKGTYIKSIFLSSTM
                     SIGVKVDPKSIDE"
     gene            complement(6824..7267)
                     /gene="rplK"
                     /locus_tag="PLG42_05485"
     CDS             complement(6824..7267)
                     /gene="rplK"
                     /locus_tag="PLG42_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOU30693.1"
                     /translation="MAKEVAGIIKLQIRGGAANPSPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKAGKIIPVIITVYSDKSFDFVIKTPPVSVQLLEAAKLQKGSPEPNRNKVATVTW
                     DQVKAIAQEKMQDLNCYTLESAMKMVAGTARSMGITIKGEFPEIK"
     gene            complement(7362..>7603)
                     /locus_tag="PLG42_05490"
     CDS             complement(7362..>7603)
                     /locus_tag="PLG42_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KOW motif-containing protein"
                     /protein_id="HOU30694.1"
                     /translation="KSEINRILGKVDELNASDEELNVPFFVGETVKVIDGPFNSFTGV
                     IEEVNEEKKKLKVMVKIFGRKTPLELSFMQVEKES"
BASE COUNT         1792 a         1771 c         1554 g         2486 t
ORIGIN      
        1 cacgctcaat gagttttctg ataatgaaag gtttataaag ttcggctgcc atgtcttttg
       61 gcaaaccaca ttcatgcagt ttaagctcag ggccagccac gataaccgag cgtgccgaat
      121 agtcaacacg ttttccgagc aggttctgac ggaaccttcc ctgcttgcct ttaagactgt
      181 cgctcaacga cttcaatggc cggttggctt cagtcttcac tgcattaacc tttctggaat
      241 tatcaaaaag tgaatcaacg gcttcctgaa gcattcgctt ttcgttgcgg aggataactt
      301 caggagcttt tatttcgata aggcgtttca aacggttgtt tcttattatc accctcctgt
      361 acaggtcgtt aaggtcgctg gtggcaaacc ttccgccgtc gagaggtaca agtggtctga
      421 gttcaggcgg gataacaggg agaaccttga taatcatcca ttccggacgg ttaacatgtt
      481 ttgattccct gaaagcctca accacctgaa gccgcttcag agcctcactc tttctttgct
      541 gcgatgtttc aatgcttgcc ttgtgacgca atgaatacga cagctcatca aggtcgagcc
      601 tgctcagaag cttgtaaaga gcctctccac ccatgtcggc aataaacttg tcggggtgtg
      661 aatcatccag cagctggttt tccttcggaa gagattccat tatttcgagg tactcttcct
      721 ctgtaaggaa atcaagatat ttgattccgt cggctgcctt gattcccggg ttgaccacca
      781 catacctttc atagtatatg attgaatcga gcttttttga tggtagcccg agcaggtaac
      841 ctatcttgtt tggtattgat ctgaaatacc agatatgtgc aaccggtacc acgagggtta
      901 tatgtcccat tctttcgcgt ctcactttct tctcagtgac ttccactccg cagcggtcgc
      961 atacaatgcc tttgtaacgt attcttttgt atttgccgca atggcattca taatctttta
     1021 caggcccgaa aatcctctca cagaacaaac catcacgttc aggcttgtaa gtgcggtagt
     1081 tgattgtttc aggtttaaga acctctccgc ttgagcggtc aagaatctct tcaggcgatg
     1141 caagtgctat cgaaatcctt gagtaacttg tttttacttt ggtgtccttt ctgaatgaca
     1201 tatattgtat ttttaaattt cagataaaaa tgactttatt gtatcagtca agcataacac
     1261 tcagtccgag gcctctcagt tcatggagaa gcacgttgag tgattcgggt atacccggca
     1321 caggcattgg ctcgcctttg acaatagctt cgtaggcttt tgccctgcct attacatcgt
     1381 ccgacttgat ggtaagtatc tcctgaagaa tatgagcagc gccgaaggct tcgagtgccc
     1441 atacctccat ctcaccgaac ctctgtccac caaactgtgc ctttcctcca agcggctgct
     1501 gggtaataag cgaataagga cctattgacc ttgcatgcat cttgtcgtca accatatgac
     1561 caagcttcat catgtaaata acacctacag ttgcaggctg gtcaaatctt tcacctgtcc
     1621 cgccatcata cagataggtg gtaccattct ttggaagccc tgccttctca acatattcat
     1681 taatctggtc gagtgtggca ccgtcaaaaa tcgggctcga gaatttcaga cccagcttct
     1741 gtccggccca cgaaagaaca gtctcataaa tctggcccag attcattcgc gacggcacac
     1801 caagaggatt tagaacaata tcaacaggcg ttccatcttc aaggaatggc atgtcctcaa
     1861 cacgcacaat ctttgcaaca atacccttgt ttccatgtct gcctgccagt ttgtctccaa
     1921 cttttatctt gcgtttcttg gctatataaa cctttgccag ctgaacaata cctgcaggaa
     1981 gctcatctcc tattgtgatg ttgtatttct tccgcttata tacaccgtcg atttccttgt
     2041 atttgataat ataattgtgt ataagcttac ggatactgtc attcttcttt ttatctgtcg
     2101 tccacttgtt ggggttaatg ttcagataat caatctcctg aagctgttta agggtaaact
     2161 tggccccttt cggtataata tcgacattta aataatcctt aacaccctgt gatgttttac
     2221 cgtttaccag aataaagagt ttgtcaacca gccggctttt aagttcctca acgttcttat
     2281 tgaattcagc gtcaagtttt tccagcatcg gcttttcgac gtttttggat ttacggtctt
     2341 tgatagccct tgtaaagagt ttcttatcaa taacaacacc ctcgagtgaa ggacctgctt
     2401 tcaatgaagc gtcttttacg tcaccggctt tgtcgccgaa gatagccctt aggagttttt
     2461 cctccggtga aggatctgat tcacctttag gtgtgatttt cccaatcaga atatcaccag
     2521 gtcttacatg agctcctatt cggatgatac cattttcatc gaggtctttt gttgcctctt
     2581 cgctaacatt cgggatgtcg gaggtaagct cctcaagtcc tctctttgta tcccttacct
     2641 ccagcaggta ctcgtcaata tgtatggatg tgaagaggtc ttcctgaacg actctttctg
     2701 aaataactat agcatcctca aagttataac ccttccaggg catgaatgca accataaggt
     2761 tgcgtccaag agcgagttct ccgtcttttg tgccgaagcc ttcggtaaga acctgtcctt
     2821 ttttcacctt ttcacccttt cttactatcg gggtaaggtt gatacatgta ttctggtttg
     2881 tttttctgta tttcggcaga taataatgtt tcacgtcctc atcgaagctg acaaattttt
     2941 cttcgtcggt acgtgtatat ctgataacta tttcgttggc atcgacatat tcaaccactc
     3001 cgtcaccttc agcaacaata agtattctgc tgtcctgtat cacctgcttc tccagccccg
     3061 ttcctactat aggtgtttcg ggtcggataa gcggtacagc ctggcgcatc atgtttgatc
     3121 ccataagtgc cctgttggca tcatcgtgtt cgaggaacgg aatcagtgat gcggctattg
     3181 atgctatctg gtttggtgca atatcgataa agtcaacgtt tgaaggttct tcatttgtat
     3241 agtcagctcc catacggcat tttaccctct gttccttgaa tgagccgtct tcattgagtg
     3301 gtgcatttgc ctgtgctatt ctcttccctt cctcttcctc ggcattaagc catacaatac
     3361 cttcatcact gaaatcaact tttccgttaa caaccctgcg gtatggagat tcaataaaac
     3421 cgaggtcgtt tattttggca tagacgcaga gtgaagatat aagtccgatg tttggacctt
     3481 ctggtgtttc tatcgggcag agacggccgt agtgggtata gtgaacgtcg cggacttcaa
     3541 aacctgctct ttctctcgac agaccgccag ggccaagagc cgacacacgt ctcttgtgtg
     3601 tcatctcagc aagcgggttg gtctggtcca tgaactgtga gagctggttt gttccgaaga
     3661 atgaattaat aaccgatgaa agcgtctttg agttaatgag gtcgataggg gtaaagactt
     3721 cattatccct tacgttcatt ctctccctga tggtacgtgc catacgggca agtcccacgc
     3781 tgaactgtgc agagagctgc tctcctactg tgcgcactct cctgttgctg aggtggtcaa
     3841 tatcgtcaac ctcagttttg gagtttatca gctcgataag atatattatg atcttgataa
     3901 tatcttcctt ggtaagaact tttatgtttg gatcgatatc caggccaagt tttttgttga
     3961 tcctgtatct gcctacatct ccaaggtcgt agcgtttgtc ggagaagaaa agtttttcta
     4021 tcacatccct tgcagtagct tcgtcgggcg gttctgcatt gcgaagctgt ctgtatatgt
     4081 aaagcactgc ttccttttcc gagttgcagg gatctttctg cagggtatta tatattattg
     4141 cgtaatcgga gaggcttaca tcttccctgt gaagaagtat ggttttagct cccgattcga
     4201 cgataaggtc gacatgttcg ggttcgataa ttgtttccct gtcgagaata atttcattac
     4261 gttcaattga aactacttcg ccggtatctt catcaacgaa gtcttctatc catgttctga
     4321 gaactctggc agcaagctta cgtcctataa attttttgag gcttgctttc gacaccttat
     4381 gttcttctgc caggttgaat atttcaagaa tttgtttgtc agtttcgaag cctattgctc
     4441 ttagcagtgt ggtgacaggc agtttcttct tcctgtcgat ataggcatac atcacgttat
     4501 ttatatcggt agcaaactct atccatgagc ctttaaatgg aattattctt gctgagtaga
     4561 gtttagtgcc gttggggtgg atactctggc cgaaaaatac gccaggggag cggtgcagct
     4621 gagaaaccac aactctttcg gcgccgttga taatgaatgt gcccctgtca gtcatgtatg
     4681 gaattgtacc cagatacaca tcctgaatta cggtatcaaa atcttcgtgt tccgggtcgg
     4741 tacaataaag tttcagcttt gccttcagcg gtacgctgta ggtaagtcct ctttctatac
     4801 attcctcaac tgtataacgc ggagggtcaa tcgaatagtc gaggaattca agtacaaagt
     4861 tgtttcttgt gtctgtgatc gggaagtttt cactgaagac cttatagagg ccttcttttt
     4921 ttctgttttc aggtgttgtt ccgagctgga agaattccct gaacgattta atctgcactt
     4981 ccagaaaatc agggtagccc ggctgttctt tccttgatgc aaaactaatt ctttcggtga
     5041 ctttcgagga catttatatt atattttaat gaacatacaa aaaataagtg acaggcttag
     5101 tcccgatagc tatcgggact aaacctgtga accggtattc cggtattggc tttatttaat
     5161 ctcaacttca gctcctgctt cagtcagttt atttttgata ttttcggctt cttccttggt
     5221 aacgccttcc ttaatggggc ccggagcctt gtcaacaagt tccttagcct ccttaagtcc
     5281 gagacctgta aggtctttaa ccagtttcac cacctgtaat ttctgaccgc cagctgattt
     5341 gaggataacg tcgaaggatg tcttctcctc tgcagctggt gctgcaggac ctgctcctgc
     5401 tgctgccggt gctgcaactg ctgctactgc tgctgcaggc tcaataccat actcttcctt
     5461 aagtatttta gcaagttcgt ttacgtcctt aaccgatagg tttaccagtt cttctgccag
     5521 ttttttaaga tctgccattt ttattgattt taaattgatt taactgatta ttactattct
     5581 ttttttgata gtgtctcaag tacaccatgt aatttcgtac tgcctgattg caaggcggat
     5641 atgacatttc tggcgggtga ctgcagaagc agtattatat cgcccagaag ttcttctttc
     5701 gatttaatag aaacaagcac atcaagcatc ttgtctccga tataaaccga ttcctgcaca
     5761 tatgcgcctt tcagaacagg cttgtcatgc tgcttgcgga attctttgat aactctggcc
     5821 ggaatggtgg ccgattttgc aaacatgaca gaggtagcac cttttagaac cggaaatagt
     5881 tcttcaaagt tgccattaac ctgctcaagt gctcttttca gaagggtatt tttcacaaca
     5941 accagtttta tctggttttc gaagcatttt cttctgagag cgcttgtgtc agcagcgtta
     6001 agatgggcta tatcagtcag gtaaaaatga ttgtattccc tgatataatt agcaaggctt
     6061 tctacaattg catttttctc ttcacgtttc ataacttctc ctttttcatt tcaggttact
     6121 catctatgct tttcgggtct actttaacgc caatgctcat cgtgcttgaa aggaatatac
     6181 tctttatata ggttcctttt gcagcaggtg gtttaagttt attaaccatt gagatgaact
     6241 ctctggcgtt atctacaatt tttgaaggct cgaaagagac ctttccgatt gacgcatgga
     6301 tgattccgtt tttatcgacc ttgaaatcga ttttaccctg tttaacttct ttaacagcct
     6361 tacctatatc catggttacg gtcccatttt tcggattagg cattaatccg cgaggaccga
     6421 gtattttacc cagctgacca attttaccca tcacagaggg catggttatg attacatcca
     6481 catctgtcca gcctcccttt atcttttcga tataatcatc gagaccaaca taatcggcgc
     6541 cagcttcgcg ggcttcggct tccttatcgg gagtgcacag aaccagaaca cgcgtctgtt
     6601 ttccagtgcc atgtggcagg gaaacaacgc cacgaaccat ctggttcgac tttcgtggat
     6661 ctattcctaa ccttatatca atatcgacag aagcatcgaa ttttgttctt gtaatctctt
     6721 tcacaagctt tgcggcttct gaaagggtat acatttttgt tctgtcgact ttttcaagtg
     6781 caaacttctg attcttggta agtttagcca tttctgtttc agtttatttg atttcaggga
     6841 attcaccttt aatggttatt cccatgcttc tggcggttcc tgccaccatc ttcatcgccg
     6901 attcgagtgt ataacagttc agatcctgca tcttttcctg ggcaatagcc tttacctgat
     6961 cccaggttac cgttgccacc ttattcctgt tgggttccgg gcttcctttc tgcaattttg
     7021 cagcttccag aagctgtacc gacactggag gtgtctttat gacaaaatca aacgatttat
     7081 cagaatatac ggtaataatc accgggataa tttttccggc tttgtcctgg gttcgggcat
     7141 tgaattgctt gcagaattcc ataatgttaa cacccttgga gcccagagcc gggcccacgg
     7201 gcggtgatgg gttagctgcc ccgccacgaa tctgtagttt aataattcca gcaacttctt
     7261 ttgccatagt taaatttttg cttgtttatt gcacgagtat gacaaccact caggaagcat
     7321 tatggttatt gcagttgtca atttcttata ttcaattttt ttcaactttc cttttcaacc
     7381 tgcataaagc tgagttcaag gggggttttc cgtccgaata tcttgaccat tactttaagc
     7441 ttcttcttct cctcgttaac ttcttcgatc actcctgtaa agctgttgaa aggtccgtca
     7501 atcactttca ccgtttcacc aacgaaaaat ggaacattga gttcttcgtc acttgcattc
     7561 agttcatcaa ctttaccaag tatcctgttt atctccgact ttc
//
LOCUS       DAOSNB010000101         7512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9811_length_7512_cov_5.553440, whole genome shotgun sequence.
ACCESSION   DAOSNB010000101 DAOSNB010000000
VERSION     DAOSNB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9811_length_7512_cov_5.553440"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..600
                     /locus_tag="PLG42_05495"
     CDS             70..600
                     /locus_tag="PLG42_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30695.1"
                     /translation="MKKKNIYFHEIDLKELRIRNCERKKFVKRLTDIPYLGYEVEDIS
                     DGRKIVIVKPGGKQAWGIMKKDDFFVFIYNPEEKSLWQITHNQIYEDLLEKSKIDKEA
                     TIEILKAFEKVFNGEEPDEVLKLISLSNSAGENPEALLKAYKWIWGQEDVNYPTGQGR
                     NMSWEKLKELLDSLIK"
     gene            783..1529
                     /locus_tag="PLG42_05500"
     CDS             783..1529
                     /locus_tag="PLG42_05500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOU30696.1"
                     /translation="MDRVISKYPTGKFDMIFADPPYFLSNGGITCHAGKMVKVDKGEW
                     DKSKGPELNHEFNYEWLSRCQKLLKPNGTIWVSGTHHVIHSVGYAMQQLGMKILNDIT
                     WEKPNPPPNLSCRYFTHSTELIIWASKNEKSKHRFNYEQMKKINNGKQMKSVWIIPAP
                     NGDEKKFGKHPTQKPLKLLERIILASTIENDLIFDPFAGSSTTGVAAIRLNRKFVGCE
                     LDENYLSLSIKRLESTIKERITSFKFIESL"
     gene            1529..2068
                     /locus_tag="PLG42_05505"
     CDS             1529..2068
                     /locus_tag="PLG42_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014710333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30697.1"
                     /translation="MKAGGRGGASTITGLNFEKKVDLQKVIEEIPGYKLSKIPDMAGK
                     LVYFEDELVARCFRKHDFYRFLEENEINWRNLLSKKLLPDDALLVIIRNTLYIIEVKH
                     QQVTGSVDEKLQTCDFKRKQYQKLVRPLGLNVEYVYILNDWFKKPEYKDVLNYINSVN
                     CHYMFNEIPLSWLGLPSKT"
     gene            2111..3028
                     /locus_tag="PLG42_05510"
     CDS             2111..3028
                     /locus_tag="PLG42_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOU30698.1"
                     /translation="MIVPKKKIPQLLKPGDEVIIISPSYAIDYEKIENAVKFLEDWGL
                     QVRVGRNALNRFGPFAGTDEERRWDLQEAIDNRNIKAVFCSRGGYGLLRIIDRLDFRP
                     LKRNPKWFIGYSDVTVLHMWLNEVCGIASLHAEMPLHYSDNQRSNETFITLHQALFEG
                     RFSFSWEGEALRGTYAEGVLTGGNLSLIYSLIGTKGEPDTSGKILFIEDEGEYYYSVD
                     RMLTSLKLAGKLKSPAALLTGGFSGMQDGKILWGKTIKETIADIVEDYDFPVFTGCPA
                     GHINDNRALIMGGYAVIKQEGSEINLSFR"
     gene            complement(3031..4251)
                     /locus_tag="PLG42_05515"
     CDS             complement(3031..4251)
                     /locus_tag="PLG42_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOU30699.1"
                     /translation="MEEQGNRENKNYGNASFMPQEEQLETTYRHRKISIGIPCDKNDD
                     EKRIALTPEAVSILVENGNEVIIQKGAGMGANYTDKDYSEFGAIITDSPARVFSADVV
                     IKVAPFEEFEIDYMKGNQTVISYLNVLKLNEETLAKLIRKKVTAIAFEKIKDSNGVLP
                     VVESMSEICGITSILIASDYLSNHHGGKGVMLGGVTGVTPTQVIIVGASTAGEYAARA
                     ALGLGSEVMIFDESLHRLRRFQSLLGQRLKTSTFHPQVLTKALKSADVLIGAIELEDL
                     RPWYYITEEMIQLMKKRSVVIDLSIDRGGCIETTECRPLKDPMYEKHGVIHFSAWNLP
                     SRVARTASIALSNIFAPLLQNIADAGSITQLLKTDAGLRNGAYLYNGILTNETLGQKF
                     GMLSKNLDLLLSAF"
     gene            complement(4312..4740)
                     /gene="tsaE"
                     /locus_tag="PLG42_05520"
     CDS             complement(4312..4740)
                     /gene="tsaE"
                     /locus_tag="PLG42_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008566205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOU30700.1"
                     /translation="MINANFKIIISELKHLPRAAEDILAVAGSRRIFAFSGPLGSGKT
                     TIIKAICKKLGAVDSVTSPSFTLVNEYLTDSGEPVYHIDLYRIKNISEAYDFGIEEYL
                     YGTSYCFIEWPEIIEELLPENTVRIKISVGPQEERILDFE"
     gene            complement(4737..6296)
                     /locus_tag="PLG42_05525"
     CDS             complement(4737..6296)
                     /locus_tag="PLG42_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOU30701.1"
                     /translation="MRKHKILWVDDEIEILKPHIYFLSEKGYEITTCSNGNDAVELVR
                     TNIYDLILLDEHMAGLSGLETLRFIKEIKPEIPVVMITKSEEENLMEAAIGSEIADYL
                     IKPVKPNQILLALKKNLEQKKLITEKTTIDYQKEFGKISDMIARASTFNDWIEIYKKI
                     VFWELELDKLPETGMNQILQTQTDEANTAFCRFVSRNYLSWLDPAVSEKPLLSHQLMQ
                     KKIFPELSNSQPVFFILIDNLRYDQWKTIQAELTELFRIISEIMYLSILPTTTQFSRN
                     AIFSGLMPLSISQTMPEMWIADNIEDERKNEFEEEFLKRLIQRAGLNIKWSYHKINNS
                     LEGKKLNEKLTSLLENNLNVLVYNFVDILSHARTESGVIRDLAYDERAYRSLTKSWFL
                     HSSLLELLKTLSEKKVRIVFTTDHGTIRVNNPVKVIGDRETSPSLRYKAGRSLDYDPS
                     EVFEIKAPEKAFLPKTNLSSKYIFAMNRDYLIYANNFNHYAAYYRDTFQHGGISMQEM
                     ILPLAFLEPVI"
     gene            complement(6310..>7512)
                     /locus_tag="PLG42_05530"
     CDS             complement(6310..>7512)
                     /locus_tag="PLG42_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30702.1"
                     /translation="TFGRTEFRRRWGDRQLEDNWRRLNKASRTALPETAEGAAAIQNG
                     KQNEIPPEADNKKPEYYLRDLPLNDSLLRVSNQKIAIALLEAGKIYHENFADNKKATV
                     TIENLIQRFPGSSLEPDAFYTLYNIYREENNTPMAEIYRQRLIEKYPESEFAKIISDP
                     SYFNRKLEENRQAEKLYEEAYNAWLNENFSTAVALCNDGLSKFSLHELAPKFQLLKSY
                     CVARTEDERTFKEELNKVIKSWPDTPEAKRAEELIAFLNQEIPELKIEEDKQTAKEIY
                     TEDKDSPHLFVLAIIDPSFNINQATFDVISYNIDNYTNRNFRTTGELVENKYIMITVA
                     GFKDLNEAMEYYNAFNVEKEIRNQTGAKIIKFIIGRNNFAALQKDMNPDRYRVYFVDN
                     YLGGKDDK"
BASE COUNT         2181 a         1325 c         1546 g         2460 t
ORIGIN      
        1 caaaaaatta atcgtggctt aaatgatttt acagaaaaaa agttgcacaa ctttaattta
       61 aataactaaa tgaagaaaaa gaatatttac tttcacgaaa tagaccttaa agaattacgc
      121 attcgtaatt gtgagcgaaa gaagtttgtg aagagattaa ctgatatccc ttatcttgga
      181 tatgaagttg aagatatttc agacggacgt aaaattgtca ttgtcaaacc tgggggtaaa
      241 caagcttggg gaattatgaa aaaggatgat ttttttgttt ttatttataa tccagaagaa
      301 aaaagtctat ggcaaataac acataaccag atttatgaag atttactaga aaaatctaag
      361 attgacaaag aagcgacaat tgaaatactt aaagcttttg aaaaagtttt taatggtgag
      421 gaacctgatg aagtgcttaa gttgatatct ctttcaaact ctgcaggaga aaatccggaa
      481 gcattactta aggcctataa atggatctgg ggacaggaag atgttaatta tccgacgggg
      541 caagggagaa atatgtcctg ggaaaaactg aaagaattac ttgactcatt gataaaatga
      601 taattatttg attatttaat atataaaact ttgtaatgaa gaaaaagaag tacaagatca
      661 atgatgagga atcagttttc ccattaatag taatggaaag ttctgaatat tcacccaaca
      721 aatgtgtatt tcgtaacgat gagctgggtg tttggctcta ccatgctaat tgtatagaat
      781 ttatggatag agttattagc aaatatccca caggtaagtt tgatatgata tttgctgatc
      841 ccccttattt tttgtcaaac ggtggtatta catgccatgc cggtaaaatg gttaaagtag
      901 acaaagggga atgggataaa tcaaaagggc ccgaattaaa tcatgaattt aattatgaat
      961 ggttatcaag atgccagaag cttttaaagc ctaatggaac aatttgggtt tcagggactc
     1021 atcatgttat acattcggtt gggtatgcta tgcagcagct tggtatgaaa atcttaaatg
     1081 atattacatg ggaaaaacca aatcctcctc caaatctttc atgccgatat tttacacatt
     1141 caacagaact tattatctgg gcttcaaaga atgaaaaatc gaaacaccgg tttaattatg
     1201 agcaaatgaa gaaaattaac aatggcaaac aaatgaaatc ggtgtggatt attcctgcac
     1261 caaatggtga tgaaaaaaaa tttggtaaac atcctactca aaagccttta aaacttttag
     1321 agaggataat actagcaagc acaatagaaa atgatttaat atttgatcca tttgcaggaa
     1381 gttcaactac tggagtagct gcgataagat taaataggaa gtttgttgga tgtgaattag
     1441 acgaaaatta tctctcttta tcaataaaac gattagaatc gacgattaaa gaaagaatta
     1501 cttcttttaa atttattgaa tcattataat gaaagcaggt ggtagaggtg gggccagtac
     1561 gattactggc ctcaattttg aaaaaaaggt tgatcttcaa aaagttattg aagaaattcc
     1621 tggttataag ttgagtaaaa taccagatat ggctggcaaa ttagtatatt ttgaagatga
     1681 gttggtggcc agatgtttta gaaaacatga tttttatagg tttttagaag aaaatgaaat
     1741 aaactggcgt aatttattaa gtaaaaaatt attacctgat gacgctttgc tggtaattat
     1801 acgaaatacc ttatatataa ttgaagtaaa acatcagcaa gtaaccggtt ccgttgatga
     1861 aaagcttcaa acatgtgatt ttaaaaggaa acaatatcag aaacttgtaa gacctttagg
     1921 tttaaatgtt gaatatgtct acattctgaa tgactggttt aaaaagcctg aatataaaga
     1981 tgtcctaaat tatattaata gcgttaattg tcattatatg tttaatgaaa taccactctc
     2041 atggcttggt ttaccttcaa aaacttaagt ttaagtttat ttacttaaaa ttatctaaga
     2101 atatgaatta atgatagttc caaaaaagaa aattccacaa ttactaaaac ctggcgacga
     2161 agtcataata atctcacctt cgtatgcaat tgattatgag aagattgaaa atgccgtaaa
     2221 gttccttgag gattggggtt tgcaggtgag ggttgggagg aatgccttaa atcgtttcgg
     2281 gccgtttgca gggacggatg aggaaagacg atgggacctg caggaagcaa tcgacaatag
     2341 aaatattaag gcagtattct gctcgcgtgg tggatatggc ctgctgagaa taattgacag
     2401 gctcgatttt cgccctttga agaggaaccc taaatggttt atcggttaca gtgatgtgac
     2461 ggtacttcac atgtggctta acgaagtttg tggaatagcc tcccttcatg ctgaaatgcc
     2521 acttcattat tcagataatc agcgtagtaa tgaaaccttt ataacacttc atcaggcgtt
     2581 gtttgaaggc agattttcgt tttcgtggga aggtgaagcc ttgcgtggca cgtatgctga
     2641 aggtgtactc accggaggta acctctcact catctacagt ctgataggta caaaggggga
     2701 acctgataca agtggtaaga tactttttat tgaagatgaa ggagaatatt attacagtgt
     2761 tgacagaatg ctaacctcac tgaagcttgc cgggaagctg aaaagtcctg ctgcacttct
     2821 cacaggagga ttcagtggca tgcaggatgg caaaatctta tggggaaaaa caattaagga
     2881 gacaatagca gacattgtag aggattacga ttttccggtt tttacggggt gtccggccgg
     2941 gcacataaat gataaccgtg ccctgattat gggtggttat gcagtgatta agcaggaagg
     3001 gtctgaaata aatttgagtt tcaggtaatt tcagaatgct gaaaggagaa ggtcgaggtt
     3061 tttcgaaagc attccgaatt tctgtcccaa agtttcgttt gtaagaattc cgttgtaaag
     3121 gtatgcgccg tttcgaagac ctgcatcggt tttcagcagc tgtgttatgc ttccggcatc
     3181 ggcgatattt tgaaggagag gagcgaaaat gtttgatagt gctattgatg ctgtacgggc
     3241 aacacgtgag ggcaggttcc atgccgaaaa atggatcact ccgtgctttt catacatagg
     3301 atctttcaaa ggacggcact cagttgtttc aatacatcct ccgcggtcga tactgaggtc
     3361 tattaccaca gaacgctttt tcataagctg tatcatctct tctgtgatat agtaccatgg
     3421 tctcaaatct tcaagttcta ttgcacctat cagcacgtcg gctgatttga gtgcctttgt
     3481 aagtacctgt gggtggaatg tagaggtttt aagcctttgt ccgagcagac tctggaatct
     3541 tctgagcctg tgaagcgatt catcgaatat cataacttct gagcccaggc ccagcgcagc
     3601 gcgggcggcg tactccccgg ctgtgcttgc accaacaatt attacctgtg tgggtgtcac
     3661 acctgttact cctcccagca ttacaccttt gcctccatga tggttactga gataatcaga
     3721 ggcaatcagt atggaagtaa ttccgcagat ttcactcatt gattccacca ctggaagcac
     3781 accattcgaa tcttttattt tctcaaatgc aatggcggta actttttttc ttatcagttt
     3841 tgccagagtt tcttcattaa gtttcaggac attgagatat gaaatcaccg tctgattgcc
     3901 tttcatgtaa tcaatttcaa attcttcgaa aggagctact tttattacta catcggcact
     3961 gaagaccctt gcaggtgaat cagttataat tgctccgaac tcgctgtaat ccttatcggt
     4021 ataatttgcc cccattcctg ctcccttctg aattattacc tcattgccat tttccacgag
     4081 tattgaaacg gcttcgggtg taagggctat tcttttttca tcatcattct tgtcgcaggg
     4141 aattccaata gatattttac gatgcctgta tgttgtttca agctgttctt cctgcggcat
     4201 gaaagatgca tttccgtaat ttttgttttc tctgttaccc tgttcttcca ttttttcctg
     4261 atcagtttat aaattcaaag gtataaaatt gaaaatgcag aacaaaatac cctattcaaa
     4321 atcaagtatt ctctcttcct gtgggccgac actgattttt atcctgacgg tattttcagg
     4381 aaggagttcc tcaataattt cgggccattc tataaagcag tatgaggtac cgtataaata
     4441 ttcttcaatt ccaaaatcat aagcctcact gatgttttta attctgtaaa ggtcgatatg
     4501 ataaaccggt tcaccactat ctgtaagata ttcattaacg agtgtaaaag aaggacttgt
     4561 tacagagtcg acagctccga gttttttaca tattgccttg atgatggttg tttttcccga
     4621 tccgagaggt ccgctgaaag caaaaatacg cctgctgccc gcaactgcaa gaatatcttc
     4681 cgctgctctt ggaagatgtt tcagttcgct tattattatt ttgaaatttg catttatcat
     4741 attaccggtt caagaaatgc cagaggtaga atcatttcct gcatcgaaat ccctccatgc
     4801 tgaaaagtgt ctctataata ggctgcataa tggttaaaat tatttgcata gataaggtaa
     4861 tccctgttca ttgcaaatat atattttgaa ctcagatttg tttttggcag gaatgctttt
     4921 tcaggtgctt ttatttcaaa aacttctgac ggatcgtaat cgaggctcct tcctgcctta
     4981 tatctcagtg agggtgatgt ttccctgtca ccgattactt taacaggatt attaactctg
     5041 attgttccgt ggtcggtggt gaatacaatt cttactttct tttcggagag tgttttgagg
     5101 agttcgagaa gagatgaatg aagaaaccat gattttgtaa ggcttctgta agcacgctca
     5161 tcgtatgcga gatccctgat cacgcctgat tcagtgcggg catgagacag aatatcgaca
     5221 aaattgtaca ccagaacatt caggttattt tccagaaggg aagtcagttt ctcattcagt
     5281 tttttccctt caaggctgtt gtttatttta tggtagctcc attttatatt cagtccggcc
     5341 cgttgaatca gtcttttaag aaattcttcc tcaaattcat tttttctttc atcttctata
     5401 ttgtctgcaa tccacatttc cggcattgtc tgagagattg aaagtggcat aagacctgag
     5461 aagattgcat tccggctgaa ttgtgttgtg gttggcagaa tgctgagata cattatttcg
     5521 gaaatgattc tgaacaattc tgtcagttcg gcctggatgg ttttccactg atcgtaacgt
     5581 aaattgtcga tgagaatgaa aaaaacaggt tgagaatttg ataattccgg aaagatcttt
     5641 ttctgcatca gttggtggga gagaagaggt ttttcactta cagcagggtc gagccatgaa
     5701 agataatttc tggatacaaa gcggcagaat gcagtattgg cttcatcggt ctgggtttgc
     5761 aatatctggt tcatacccgt ttcagggagc ttgtccagtt caagctccca gaagacgatc
     5821 tttttgtata tttcgatcca gtcattaaac gttgaagctc gggctatcat atcagagatt
     5881 ttcccgaatt ctttctggta atcgattgtt gttttttcgg taataagttt cttctgttca
     5941 aggttttttt tcagtgccag cagtatctga ttcggcttaa ccggtttaat gaggtaatca
     6001 gcaatttctg aacctattgc agcttccatc aggttctcct cctcgctctt ggttatcatt
     6061 acaaccggta tttctggttt tatttccttg ataaacctta atgtctcaag accgctgagc
     6121 ccggccatat gttcatcgag aagtatcaga tcatagatat ttgttctgac aagctcaaca
     6181 gcatcattac cattggagca ggttgttatt tcatatcctt tttcactgag aaaataaata
     6241 tgaggcttga gtatttcaat ttcatcatca acccataata tcttatgttt tctcatccct
     6301 cagcaagttt tatttatcat ctttccctcc gagatagtta tcaacgaaat atactctata
     6361 tctatcagga ttcatgtctt tctgcagggc ggcaaagttg tttctgccga taataaattt
     6421 aataattttt gctccagtct ggtttctgat ttctttttca acattgaagg cattgtaata
     6481 ctccattgct tcattcaaat ctttgaatcc cgcaactgta atcataatat atttattttc
     6541 aacaagttca cccgtggttc tgaaattgcg gtttgtataa ttatctatat tatagcttat
     6601 tacatcaaaa gttgcctgat tgatgttaaa tgaaggatcg atgattgcca ggacaaaaag
     6661 gtggggcgaa tctttgtctt cggtataaat ttcctttgcg gtttgtttgt cttcttctat
     6721 tttcagttcc ggaatttcct gattaaggaa agcgataagt tcttctgccc ttttagcttc
     6781 cggggtgtcg ggccaggact ttatgacttt attaagctct tccttgaaag ttctttcatc
     6841 ttcagtgcgc gctacgcagt aacttttcag cagctggaat ttgggtgcaa gttcatgaag
     6901 tgagaatttt gaaaggccat cgttgcaaag agcaactgca gtgctgaagt tttcattaag
     6961 ccaggcgtta tatgcttctt catacagttt ttcagcctgt ctgttttctt caagttttct
     7021 gttgaagtaa gatgggtcgg aaatgatttt tgcaaattcg ctctcagggt atttttctat
     7081 aagacgttgc ctgtaaattt ccgccatcgg tgtattattt tcttccctgt atatattata
     7141 taatgtataa aatgcatcag gttcgagact gcttcccggg aacctctgga tgagattttc
     7201 tattgtaacg gtagcttttt tattgtctgc aaagttttcg tgataaattt ttcctgcttc
     7261 aagaagggcg attgcaattt tctggttcga cactctgagg agtgagtcat taagtggcag
     7321 gtctcgaagg taatattcag gctttttgtt atcggcttca ggcggtattt cgttctgttt
     7381 accattttgt atggctgctg ctccttcggc cgtttcaggt aaggctgttc tggatgcttt
     7441 gttaagtcgc ctccagttgt cttcaagctg gcgatccccc catcttctcc tgaactcagt
     7501 gcggccaaag gt
//
LOCUS       DAOSNB010000102         7443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9950_length_7443_cov_4.487006, whole genome shotgun sequence.
ACCESSION   DAOSNB010000102 DAOSNB010000000
VERSION     DAOSNB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9950_length_7443_cov_4.487006"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLG42_05535"
     CDS             <1..1018
                     /locus_tag="PLG42_05535"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOU30703.1"
                     /translation="LSGRTITNIGPFEYNSTQYNVAHPSISRDGKFLFFASDMPGGQG
                     GSDIWMCENVNGKWSVPKNLGSKVNSPSKENYPYIHPSGRLYFSSDRKGTASYMGGMD
                     VYYTVLVDGEWDDPIPMPEPINSQYDDFALCASDNLQEGFFTRKTGRDDDILRFASNI
                     IRKDVCDPVQENNYCYEFIEENAIKYDTLSVPFKFIWNFGDGETAEGSRVIHCFKGPG
                     DYTVKLDIVNLLTNEIELNEKTYDLRVEQIEQAYISGPDRCFEGEKVIFDADSTYLPG
                     WAINQFYWNFGDETIAIGTKVDKIYQRPGNYTVQLIVTSAPDGSGRVKETCVSKNIVV
                     LRKP"
     gene            1045..2043
                     /locus_tag="PLG42_05540"
     CDS             1045..2043
                     /locus_tag="PLG42_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30704.1"
                     /translation="MSRKKLTYSLVTVIIIFFCLTANSAAQPVPGEDENIQFLVTFGR
                     DGATSWGDDDFSQTWFFAIPKEYKGRFYIRVFDPDTGGEHDEPKGEWNTRTQFSVYGG
                     KGVDPEINEDSRGLQPTGNYKSGTLLASRIFGEDRQYDNKYYTFGPFNPTEGDYSEKW
                     GYIFKVIADGISGDDGNLYRYFLSREPNNDVPIEGANAFTYEYTFRMWNNTQSISHIY
                     PFIDEGVIYVKQKNFDWDDDGTIVVVSRVRKGIQVPISNEDDWVESRIGVEPEEVNSS
                     LDFQFHKKKEFLVRNNNVVISLENQRGDNLKFFSAPIGGVPIYQPRIVVRKVDKNQ"
     gene            2044..5220
                     /locus_tag="PLG42_05545"
     CDS             2044..5220
                     /locus_tag="PLG42_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOU30705.1"
                     /translation="MFRSYLYTCIVRRIGLCLAAGYLILIPELKGQIYSFKNFGASSS
                     IPDTYIYTINQDQNGFLWVGTGNGIAKFDGITFHTVPYPDSINTRYPKCSFRDRTGRI
                     WFGCSDGTLFYTLNGELVKFRELGFQSISQIVQSDEEYISVIPQEKSIVKISINDPER
                     VIKYNVPRGLIMTCAAFLTENTMLLGTTENILFCRFERDSVKIVSRIKGIEYTRIQCI
                     FPIDGAETLLIGTEGSGIYILEFKNGSPEIEKLENNTLSEELNVKSFFRGSNEEIWMA
                     TMGSGLIKFSYNSSLRNFDNVELFTVSNGLQSNDVTDVFEDSEGNIWTGLYGEGISML
                     YSRALYIYTPGDLPEKNNIINVSLEGSKYFLGTPTGFYYFDPSSGKVEGYTELRNKIN
                     IEISAYLYENSSTIWAGTRGKGLYLTGKNGQIRQVYFSENSGQNYIRHIAAEDQNLWL
                     STLDGIVVINKNTGKVIRKFNIEDRLPHNSINQIIFLQNGEALPATECDRLYRVTLSG
                     GVEIGRRVMSGITRNKVLCFAEDDHGDIWAATAGNGIFWFTGDSVNNFTTYEGLFSNF
                     CYSILADSSGQVWVGHERGFSVFDPHSGLLKKYSPDFMKSSNCNPNSIAVDHMGRILF
                     GTTEGLVIYDKRLERAASVVPKTNLISITINNIRYPLRKEYVLPYSKYTIKIEYVGIN
                     LRNPEEVYYSTKMDNWDNEWSGLKTERQTTYSLRDGKYKFNVISYNAAGQSDHTPVSV
                     EFVIKTPFWRTWYFILLVIFVIAGTVILIVRQREKAQEKVKKYLEDELEKRTSEVVKQ
                     KEMIEMQNLEITDSINYARRIQSSILPDVNKLKETFSDSFVIFYPRDIVSGDFYWFDR
                     IDNDRFMLVCADSTGHGVPGAFMSMIGATLLQDIIVRKGVTKPSQILTMLDKQIISTL
                     NQNVDVGVSNDGMDMVICEINIPERHIKLASAMRPIIIIMGGELYYIKGNKYSVGGES
                     VTEKYFDDLEYYLSKGDSVYLFSDGFPDQFGGPDGKKMKIARFKSLIEEVHSLPMAKQ
                     KEVIEKFFFDWKNDYAQVDDVLVIGIRF"
     gene            complement(5228..5746)
                     /locus_tag="PLG42_05550"
     CDS             complement(5228..5746)
                     /locus_tag="PLG42_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30706.1"
                     /translation="MINLKNIFSFLAAGLILSACVKIEHLPPEPSISFRSFEVFDTTD
                     ILGNAIKGGRLKFYFEDGDGDLGMSKQGFSDYDTINLFVNLLRVKNNEIIPAPDNDPL
                     RSSGYRIPYMERTGQNKILRGIIKVTFFYFFWSPADTIYYDFYLKDRAGHFSNTERTC
                     QIILNNNGICTR"
     gene            complement(5789..7093)
                     /gene="der"
                     /locus_tag="PLG42_05555"
     CDS             complement(5789..7093)
                     /gene="der"
                     /locus_tag="PLG42_05555"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOU30707.1"
                     /translation="MSRIVAIVGRPNVGKSTLFNRLTQSRQAIVDEESGVTRDRIYGK
                     ADWNGITFSVIDTGGYVEGSDDIFEEEINKQVLVAIEEADAIIFLTDVTTGVHDLDKS
                     VASLLRKSGKKIFLAVNKVDNNSLISAAFEFYELGLGNPYPLSSLNGSGTGELLDDLV
                     KVLPPAGVEEETELPRVAIVGRPNVGKSSLLNSLLGEERNIVTPLAGTTRDSIYTRYN
                     KFNHDFLLVDTAGLRKKSRIKNDIEFYSVMRAVKAVENSDVCLLMVDATRGIESQDIS
                     IFSLIQRNNKGMIVLVNKWDLVKKDSNTAKLFEEEIRKRTAPFTDYDILFISAINKQR
                     IHKILECIEKVNNNRKKRIPTSELNEAMQEAFRAYPPPAVRGKNIKIKYVTQLPSYSP
                     AFAFFCNYPQYIKEPYKRYLENRLRENFDFTGVPIKIFFRKK"
     gene            complement(7124..>7443)
                     /locus_tag="PLG42_05560"
     CDS             complement(7124..>7443)
                     /locus_tag="PLG42_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOU30708.1"
                     /translation="FFAAEIIREKIFLNYRQEIPYSVEVEIESFTDLENLSKIRAVIH
                     VARESQKGIIIGHQGKMLKKIGTEARKEMEEFFGRKIYLELYVKVTENWRDNPAILKR
                     FGY"
BASE COUNT         2311 a         1311 c         1558 g         2263 t
ORIGIN      
        1 gctgtcggga cggaccataa caaatatagg tccgtttgaa tacaacagta ctcaatataa
       61 tgtagcacat cccagtataa gcagggatgg caagtttctg ttctttgctt ccgatatgcc
      121 cggcgggcag ggaggttcag atatatggat gtgcgaaaat gttaacggca agtggagtgt
      181 acccaaaaac cttggcagca aggtaaattc accatctaaa gagaattatc cttatattca
      241 tccttcaggc aggctatact tttcttccga ccgaaagggc acagcttctt atatgggggg
      301 aatggatgtt tattatacag tacttgttga cggagagtgg gacgatccga ttccaatgcc
      361 cgagccaatt aactcacagt atgacgattt cgccctttgt gcatctgata atttgcagga
      421 aggctttttt acccgaaaaa ccgggcgaga tgatgatatt ttgagatttg catcaaacat
      481 cataagaaaa gacgtttgcg accctgttca ggaaaataat tattgctatg agtttatcga
      541 ggagaatgct ataaaatacg atacactttc cgtgcctttt aaatttatat ggaatttcgg
      601 cgatggtgaa acagcagaag gctcaagagt cattcattgt ttcaaaggtc ctggagatta
      661 tactgtgaag ctggatattg taaatcttct gacaaatgaa attgagctga atgaaaagac
      721 atacgatctt agggttgaac agatagaaca ggcatatatt tctggtcctg acagatgttt
      781 cgaaggagaa aaagtaattt tcgatgctga tagcacatac cttccaggat gggcaataaa
      841 tcaattttac tggaatttcg gggatgaaac aatagcaata ggcacgaaag ttgataagat
      901 atatcaaagg ccaggtaatt atacagtaca gttaatagtt acttcagcac cggatggaag
      961 cggcagggta aaggaaacat gtgtttcgaa aaatattgtg gtactccgga agccttgatt
     1021 aaaaatcatt atttttaaaa cgaaatgagc aggaagaaat taacatacag tctggttacg
     1081 gtaattatca tctttttttg cttaactgcc aattcagccg cacaacctgt tcccggtgaa
     1141 gatgaaaata ttcagttttt agtgactttt ggaagagatg gagcaacttc atggggagat
     1201 gatgatttct cccagacatg gttttttgca attcctaagg aatataaagg gagattttat
     1261 ataagggttt ttgaccccga taccggaggc gaacatgatg aacccaaagg tgaatggaat
     1321 accagaacac aattttcagt ttatggaggt aaaggtgttg atccggagat aaatgaagac
     1381 tccagaggtc tgcaaccgac cggtaattac aaaagtggca cacttctagc ttccagaatt
     1441 ttcggggaag acaggcaata tgataacaaa tattacactt tcggaccatt caatcctaca
     1501 gaaggcgatt acagcgaaaa gtggggatat attttcaagg ttattgctga cggaataagc
     1561 ggtgatgatg ggaaccttta caggtatttt ctctcacgcg aaccaaataa tgatgtgcct
     1621 atagaaggag caaatgcttt tacctatgag tatactttca gaatgtggaa caatactcaa
     1681 agcatttctc atatttaccc ttttattgat gagggagtta tatatgttaa acagaagaat
     1741 ttcgactggg atgatgacgg aactatagtt gtagtatcaa gagtcagaaa aggtattcag
     1801 gtgccaatct caaatgaaga cgactgggtg gaatcaagaa tcggcgtgga gcctgaagag
     1861 gtaaattcat cactcgattt tcagtttcac aagaaaaagg aatttctggt tagaaacaac
     1921 aatgttgtga tatcactcga aaaccagaga ggtgataatc ttaaattctt cagcgcccct
     1981 atcggaggag ttcctattta tcagcccaga attgttgtgc gtaaagtaga taaaaaccag
     2041 taaatgttca ggagttattt atatacctgt attgtacgaa ggattggttt atgcctggct
     2101 gcaggatatt taattcttat tccggaactt aaagggcaaa tctatagttt taaaaatttc
     2161 ggggcatcaa gcagtatacc cgatacttat atttatacta taaatcagga ccagaatggc
     2221 ttcctctggg ttggtacagg aaacggaatt gcaaagttcg acggaataac ctttcataca
     2281 gttccttatc ccgacagtat caatacacga tatcctaaat gcagtttcag agacagaaca
     2341 ggcaggatat ggttcggttg tagcgacggc actctgtttt atactttgaa cggtgaactg
     2401 gtcaagttca gggagcttgg attccagagc attagccaga ttgttcagtc ggatgaagaa
     2461 tatatttctg ttatacctca ggaaaagtcg atagtgaaaa ttagcattaa tgatcctgaa
     2521 agagttataa aatataatgt tcccagaggt ttgataatga catgcgcagc atttctaaca
     2581 gaaaacacca tgcttctcgg aactacggag aatattcttt tttgcaggtt tgaaagggat
     2641 tcagtgaaga ttgtaagcag gatcaaaggg attgaataca caagaataca atgtattttc
     2701 cctatcgatg gagctgaaac acttttaatt ggtactgaag gatcaggtat ctacattctg
     2761 gaatttaaaa acgggagccc ggaaatagaa aagctggaaa ataatacatt gtcagaagag
     2821 ctcaatgtaa aatccttttt cagaggttca aacgaagaga tatggatggc taccatgggt
     2881 tcgggactta ttaaattttc ttataattcc agcttaagaa attttgataa cgttgaacta
     2941 ttcactgtca gcaatggatt gcagagcaat gatgtgacag atgttttcga agattctgaa
     3001 ggaaatatct ggacgggatt atacggtgag ggtatttcaa tgctttattc acgtgctctt
     3061 tatatttaca ctccgggtga tttaccggaa aaaaacaata ttatcaatgt aagccttgaa
     3121 ggttctaaat attttcttgg cacccctacc ggcttttatt attttgatcc ttcctctggc
     3181 aaagttgaag gatatactga attaaggaat aaaataaata tagagatatc tgcatacctg
     3241 tatgaaaatt cctccactat ctgggctgga acacggggta aaggattata tctgacaggc
     3301 aaaaatggac agattcgaca ggtttatttt tccgaaaatt caggacagaa ttatatccgg
     3361 catatagctg cagaggacca gaatctttgg ctttcaactc ttgacggaat tgttgttatt
     3421 aacaaaaata caggtaaagt aatcagaaaa ttcaatattg aagacagact gccccataac
     3481 agtattaacc agataatttt tctgcaaaat ggagaggctt taccggcaac agagtgtgat
     3541 aggctctata gagtaacgct gagcggaggt gtggaaatag gaagaagggt aatgtcgggg
     3601 attacaagaa ataaggttct atgttttgct gaagatgatc atggtgatat atgggcagca
     3661 acggcgggca atggaatttt ctggttcacc ggcgattcag taaataattt tacaacttac
     3721 gaaggactct tttccaattt ctgctacagc attcttgccg acagtagcgg acaggtttgg
     3781 gtaggacatg aaagaggctt ttcagttttt gatcctcatt cagggttgct aaagaaatat
     3841 tcgcccgatt ttatgaaaag ctccaattgc aatcccaatt caatagcagt ggatcatatg
     3901 gggagaatat tgtttggaac cacggaagga ctggtaattt acgataaaag gttggaaagg
     3961 gcggcgtctg tagtcccgaa aactaattta atttcaatta ctattaataa tataaggtat
     4021 ccactgagaa aagaatatgt tttgccatac agtaaataca caataaaaat tgaatatgtt
     4081 ggaataaatc ttagaaatcc cgaagaagtt tattacagca caaaaatgga taactgggat
     4141 aatgagtggt cgggattgaa aacagaaaga caaactacat attcattacg cgatggaaaa
     4201 tacaagttta atgtaatttc gtataatgct gctgggcagt cagatcacac acctgtatct
     4261 gttgaatttg taataaaaac ccccttctgg agaacatggt attttatact gctggtgatt
     4321 tttgtcatag caggtacagt gattctaata gtaagacaga gagagaaagc tcaggaaaag
     4381 gtaaagaaat accttgagga tgaactggaa aagagaacca gtgaggtggt taaacagaag
     4441 gaaatgattg agatgcagaa tttggaaatt actgatagta tcaattatgc cagaaggatt
     4501 cagtcaagca ttttgcccga tgtaaacaaa cttaaggaga cattttctga cagttttgta
     4561 attttctacc cgagagatat tgttagtggg gatttttact ggttcgaccg cattgataat
     4621 gataggttta tgcttgtctg tgctgattcg acaggtcatg gtgttccggg tgcatttatg
     4681 tcaatgattg gagcgactct tttacaggat ataattgtca ggaaaggtgt tacaaaacct
     4741 tcacagattt taacgatgct cgataaacag ataatttcaa cactgaacca gaatgttgat
     4801 gttggtgttt caaacgatgg gatggatatg gtgatctgtg aaatcaatat tcctgagcgg
     4861 cacataaaac ttgcatctgc aatgcgcccc ataataataa ttatgggggg agaactctac
     4921 tatataaaag gcaataaata ctcagtggga ggtgaatcgg ttaccgagaa atattttgat
     4981 gatcttgaat attacctcag caaaggcgat agcgtttatc tgttttccga cggtttccct
     5041 gatcagtttg gcggtcctga tggtaaaaaa atgaaaattg ccaggtttaa gtcacttatt
     5101 gaagaagtcc atagtcttcc gatggcaaag cagaaggagg taattgaaaa gttcttcttc
     5161 gattggaaaa atgattacgc tcaggttgat gatgttctgg ttatcgggat aaggttctga
     5221 atattgttta cctcgtgcag atgccgttat tatttaatat tatctgacag gttctttccg
     5281 tattactgaa atgacctgca cggtctttca aataaaaatc gtaatagatt gtatcagccg
     5341 gtgaccagaa aaaataaaag aaagtaacct ttattattcc tcttagaatt ttattctgac
     5401 ctgttctttc catgtaaggt attctgtacc cagaagatct cagaggatca ttatccggtg
     5461 caggtataat ttcattattc ttgaccctca acaggttgac aaacaaattt atggtgtcat
     5521 aatccgaaaa accctgcttg ctcattccaa gatcgccgtc accatcctca aaataaaatt
     5581 taagcctgcc tccctttatc gcgttcccaa gtatatcagt tgtgtcaaaa acttcaaaac
     5641 ttctgaaact gattgacggt tccggtggca aatgctctat tttaacacag gctgacaata
     5701 tcagacctgc tgcaagaaaa gaaaagatat tttttaagtt tatcattatt gattatgtct
     5761 taaaaatgtc ttcaaaaatc aaaccaattt atttttttct gaaaaatatt tttatcggaa
     5821 caccggtaaa atcaaaattt tccctcaatc tgttttcaag ataacgttta taaggctctt
     5881 ttatgtattg agggtagtta caaaaaaatg caaaagccgg tgaatatgaa ggcagctggg
     5941 ttacgtattt aattttaata ttctttcctc ttactgccgg aggcggataa gccctgaaag
     6001 cttcctgcat tgcttcatta agctcggatg tcggtattct ctttttgcgg ttattattga
     6061 ccttctctat gcattcaagt atcttgtgga tacgctgttt attgattgct gagataaaca
     6121 gaatatcata gtcggtaaaa ggggctgtac gttttcttat ttcttcttca aataattttg
     6181 cggtatttga atcctttttt acaaggtccc atttgtttac aagaactatc atccccttgt
     6241 tgtttcgctg aattaatgaa aatatgctaa tatcctgaga ttcgatacca cgtgttgcat
     6301 ccaccattag caggcatacg tcagaatttt ccacagcttt tacggccctc atcacagaat
     6361 agaattcaat gtcatttttt attctgcttt tctttctgag tcctgccgtg tcaaccagaa
     6421 ggaaatcgtg attgaatttg ttgtatcgtg tatatataga gtctcttgtg gttcctgcaa
     6481 ggggtgtaac tatgtttctt tcttctccaa gcagggagtt aaggagtgac gatttaccca
     6541 cattggggcg acctacgatt gccacccttg gcagttctgt ttcttcctcc accccagcag
     6601 gaggcagtac ttttacaagg tcgtcaagca gttcacctgt gccgcttccg ttcagtgaac
     6661 tcaatggata aggatttccg agcccgagtt cataaaactc gaaagctgca ctgataagtg
     6721 agttgttatc aactttattg actgcaagaa agattttttt tcctgatttc cggagaagtg
     6781 atgctacaga tttatcaagg tcgtgaacgc ctgttgttac gtcagtaaga aatataatag
     6841 catcggcttc ctcaatggca accagtacct gtttatttat ttcttcttcg aaaatatcgt
     6901 cagagccttc cacatatccc ccggtatcaa taaccgaaaa agtaataccg ttccaatcgg
     6961 ctttaccata tatacggtca cgggtaaccc cactctcctc atcaactatt gcctgtcggc
     7021 tctgagtcag cctgttgaag agagttgact ttccaacgtt aggtcgtccg actatggcaa
     7081 ctatcctgct cataagttaa tgattgtcag atagtttaaa ttgctaataa ccaaaccttt
     7141 ttagaattgc aggattatcc cgccagtttt cggtcacttt aacatacagt tcaaggtata
     7201 ttttccgtcc aaaaaattcc tccatttctt ttctggcttc ggttcctatc ttttttagca
     7261 ttttcccctg atgacctatg ataattcctt tctgactttc tcttgcaacg tgtataacag
     7321 ctcttatttt gctcaggttt tccaggtctg taaaactctc tatttcaacc tcaactgaat
     7381 aaggtatctc ctgacggtaa tttaagaata ttttttctct gattatttct gccgcaaaaa
     7441 acc
//
LOCUS       DAOSNB010000103         7442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9954_length_7442_cov_4.959930, whole genome shotgun sequence.
ACCESSION   DAOSNB010000103 DAOSNB010000000
VERSION     DAOSNB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9954_length_7442_cov_4.959930"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLG42_05565"
     CDS             <1..233
                     /locus_tag="PLG42_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOU30709.1"
                     /translation="AMLIMLFNAIKWHGGARILLISIVAVLLFLIISLWEIIKSSRIN
                     TAEKILWIICLLFLGVLGEIIYLIAGRRKIAL"
     gene            complement(447..845)
                     /locus_tag="PLG42_05570"
     CDS             complement(447..845)
                     /locus_tag="PLG42_05570"
                     /inference="COORDINATES: protein motif:HMM:NF021462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HOU30710.1"
                     /translation="MFIRLYLIALPVFFVIDMIWLVLIARNFYKEQIGFIMKPEINWV
                     AAILFYLLFIAGLVVFVISPAVEKRTWIHALGYGALFGLVTYATYDLTNLATLKEWPL
                     KVTIIDLIWGTLLASSVSVVTYFIAIKLFR"
     gene            1024..1665
                     /locus_tag="PLG42_05575"
     CDS             1024..1665
                     /locus_tag="PLG42_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOU30711.1"
                     /translation="MTPRTAIQLEKIREAKRALIMQSALEHFAKNGYHATTISHIAKH
                     AGISKGLMYNYFPSKEALLKEIIYNYIQEIYQYFDINRDGYLTEEEFEYFIHQIYRTL
                     KEKKSFFQLLFQMMMQSEVRLKLIELQKSIETLAARSDDSITALEKQIYKTLSDYFKR
                     KKEKTGQEYDSDMELNIFIYTIKGFTLTYVFNETEFDEEYFEKSVNKIIELYK"
     gene            1673..2434
                     /locus_tag="PLG42_05580"
     CDS             1673..2434
                     /locus_tag="PLG42_05580"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU30712.1"
                     /translation="MKKYLLLQGFFLWLISAAQVTAQGLSASEIVRKADEKFNGEKSS
                     YSIMSMTIVRPTWQRTIEFKSWTLGRDYALTLITAPARDAGQSFLKRKNEMWNWNPSI
                     NRLIKLPPSMMSQGWMGSDYTNDDIMRESSVVNDYEHEFAGEEVINGRECYKIRMTAK
                     KDASVIWGQQIRWIDKKDFLFLKAELYDEDGMLVRTETGSEIKMMDGRNIPTRIELIP
                     SEEQGKKTIVTIKEIKFNIPVTEDFFSQQNMRTIR"
     gene            2477..3898
                     /locus_tag="PLG42_05585"
     CDS             2477..3898
                     /locus_tag="PLG42_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU30713.1"
                     /translation="MRDNFKIAWRNLWRNRRRTLITASSVFFAVFFAIIMRSYQLGTY
                     DHMISNVIESYTGHLQIQHPEYQNNPQIDNSFDFNDSLFLLISNVENVVSVTPHLEFF
                     ALASNGPQTKGVAILGIDPAREKTFSNPENKLVRYRITDEALLEMTQSGTFPASVLKK
                     LQENKDRSYSSRARLELELGLAITETEKYIPEIIRFCEVQNGFLNEEDDGILVSDRLA
                     RYLKAEIGDTIILIGQGYQGASAAGLFPVRGIIKMPAPDLDNRLIMMTIPAAQKFLDA
                     PGKITSVSINLTDRSTRTIKKALDRINKLMEGMGTTVKTWYELNPVLYQQIQGDSQSG
                     MVMLGVLYFIIFFGIFGTVIMMVAERKKEFGVLISIGMQKTRLKRIISMEMAMLGLLG
                     LAGGMLAAVPFILYFFYNPIVLKGDLGKMMEDMGWDAIMPAKWFGPYYYIQALIVVIM
                     ILLATLYPLRKINKLKEVEALRS"
     gene            3914..5338
                     /locus_tag="PLG42_05590"
     CDS             3914..5338
                     /locus_tag="PLG42_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOU30714.1"
                     /translation="MIPEISWKNIWRNKTRSLIVITAVTLGIISGVLLVGIMQGWVEQ
                     RIHDAIHNEVSHVQIHNAEYYDNEDILSTIDSLNKIISVLESDPEVKAWTERTKLTAI
                     ATTPWASTGLFIYGINPQKEKEVSEIYTKIVPNGGEYPDINKTGYILISDKTAEILQL
                     KQYIITEPLIEKFRESRLPDNVVIKLDSIKGIRFRSPKEFKAILEKKLNRKELDMYGK
                     LIMDSALDYRIRNRIQITLSDCNGNPVQGTFRVCGIYKTTNTGFDQASAFVNINELAE
                     LYGSDTPLVHEIAIMLHNAENIDNVKNKLSTVSDKNIVHTWKELAPDAAMMNDFMYMY
                     YFIFIGIIMLALAFGIINTMLMAILERTKELGMLMAIGMNRKRIFSMIMLETIFLTSA
                     GAVAGMLAGWVLVSMLGKTGIHFSSWGEGFEAIGYSANVYPVVTFDFFIFVIAMVVFT
                     AVLSSIWPARKALKLNPVETLRTE"
     gene            5354..6052
                     /locus_tag="PLG42_05595"
     CDS             5354..6052
                     /locus_tag="PLG42_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU30715.1"
                     /translation="MKILELKKVNKIYDGSEVPVHAVRDVTLEFEEGEFAAIVGPSGS
                     GKTTLLNLIGGLDMPTSGEIIIDGVNLAKLKPSQLIDFRLRNIGFVFQSYNLIPVLTA
                     RENVEFIMSLQKWPRKERDARTYELLKAVGLEERMNSRPSKLSGGQQQRVAVARALAP
                     KPKFILADEPTANLDSKAASNLLEIMEKLNREEKITFIFSTHDPRVVKKARRVITLED
                     GRVFSDEKIQSVND"
     gene            6057..7211
                     /locus_tag="PLG42_05600"
     CDS             6057..7211
                     /locus_tag="PLG42_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30716.1"
                     /translation="MIKPPVLSIIAVIIAFNVNNIAGQEKEKPVTLKGYVTTLQNAMF
                     DSLSGPVSYNNILHNRLNFKGYINNNIILGIELRNRLFTGDLARTGNFYSDITGRDNG
                     WFDMSWNVIKENSFFLNTTIDRLWIAMKLGKLEITTGRQRINWGQAIVWNPNDIFNAY
                     SFFDFDYVERPGSDALRFQYFTSSSSIVELAVKADSEDRVTAAGLIRFNRWGYDIQFL
                     GGYSNSSDIVAGAGWSGTLLNTSFRGEISWFYPVNKGEKSTLIATAGIDKIFYDNSVL
                     QIELMYSGNPVALNDINILYSGTLSAKDLAFSKFTAFGSFTWALTPLLNTGISAMWFP
                     DLKGYFAGPSLDYSMSENIDLSVIWQHFNIHTGSIKTRMNLAFLRLKYSF"
BASE COUNT         2532 a         1347 c         1429 g         2134 t
ORIGIN      
        1 ttgcaatgct gattatgctg ttcaacgcca ttaaatggca tggaggggcc agaatactgt
       61 taattagcat agtggctgtt ctcttatttc tgataatttc attatgggaa attattaaat
      121 caagccgcat aaatactgca gaaaaaattt tgtggattat ttgtctgctt tttctcggtg
      181 ttctgggcga aatcatttat ctaattgcag gaagacgtaa aattgctcta tgataatatt
      241 aaagctgctt gacatttttc agtttattgt tactaaactt atttactcgc agcaaaacgc
      301 catatcttaa taattcaact aacagggctg aggccaaaaa taattctgaa aactaaataa
      361 agcttcttaa taatgcttaa tatattcttt gtgtttttgc caattgaaat tcaattaaat
      421 atatctttaa acttaggtga tattaattaa cgaaagagtt taatggcaat aaaataagtc
      481 acgacagaga cagaagatgc cagcaatgtg ccccaaatta gatcaatgat agtaaccttc
      541 agaggccact ccttaagagt ggcaagattt gtcaggtcgt atgtggcata agttaccaga
      601 ccgaataatg ctccgtatcc gagtgcatga atccacgtgc gtttctcaac agctggtgaa
      661 ataacaaata ctacaaggcc agctataaac agcaggtaaa acaaaatagc agcgacccag
      721 ttgatttcag gtttcataat gaaacctatt tgttccttgt aaaaattacg ggcaataagc
      781 acaagccata tcatatcaat cacaaagaat accggaagtg caatcagata taatcttata
      841 aacatcttac gattaattta ttatatcaca ctataaattt actctttttt ctttcaagaa
      901 ttaaaaaagt attttaaaaa tcttttgcat cttaaaattt atttctatat ttgactgacc
      961 gttcattcat aaaatacttt tgcatcacat aattcagaaa acaattattg tattaccgac
     1021 tatatgacac ccagaaccgc tatacaactt gagaagatac gggaagcaaa aagagcactt
     1081 attatgcaat cggcgctgga gcattttgca aaaaacggat atcatgccac cactatcagc
     1141 catatagcaa aacatgcagg tatttccaag ggcctgatgt acaactattt tccaagcaaa
     1201 gaagctttac ttaaggaaat aatatacaac tacatacaag aaatttacca gtattttgac
     1261 ataaatcgcg atgggtacct caccgaagag gaatttgaat atttcataca ccagatttac
     1321 agaacactaa aagaaaagaa aagcttcttt caattacttt ttcagatgat gatgcagagt
     1381 gaggttcggt taaagcttat agaattgcaa aaaagtatag aaacactggc cgcaaggtca
     1441 gatgattcta taactgctct cgaaaaacag atatataaaa cattatctga ttatttcaaa
     1501 agaaaaaagg aaaagaccgg tcaggaatat gattctgata tggaattgaa tatatttatt
     1561 tacacaatta aaggatttac actcacctat gtttttaatg aaactgaatt tgatgaagaa
     1621 tatttcgaaa aatcagtaaa caaaataata gagctttata aatagattta taatgaagaa
     1681 atatctactg ttgcaaggat tttttttgtg gttgatttcg gctgcccagg tcaccgctca
     1741 gggcttatca gcatcagaaa ttgtaaggaa agccgatgaa aaatttaatg gtgaaaagtc
     1801 aagttattcc attatgtcca tgactattgt aagaccaaca tggcagagaa ctatcgaatt
     1861 caaaagctgg acactcggga gggattacgc tctcacactt ataacagctc cagccagaga
     1921 tgcagggcag tcattcctga aaagaaaaaa tgaaatgtgg aactggaatc cctcaataaa
     1981 ccgccttatt aaactccctc cttcaatgat gtcgcaggga tggatgggct cagactatac
     2041 aaatgatgac ataatgagag aatcttccgt tgtgaatgac tatgagcacg aatttgcagg
     2101 cgaagaagtc attaatggcc gtgaatgtta caaaatcaga atgacagcaa aaaaagatgc
     2161 ttcagttatc tggggtcaac aaataaggtg gattgacaaa aaggattttc tatttcttaa
     2221 ggccgaatta tatgacgaag acggaatgct ggtaagaacc gaaaccggat cagaaataaa
     2281 aatgatggat ggcagaaata ttccaacaag aatcgaactt atcccttcag aagaacaagg
     2341 gaagaaaaca atcgttacaa ttaaagaaat aaaattcaat atacccgtta ccgaagattt
     2401 tttttcacag cagaatatga gaacaatcag atgataaaac catgtcatct atttgtctga
     2461 taataaaaat gacataatga gagataattt caaaatagcg tggagaaacc tctggagaaa
     2521 caggagaaga acgttgatta ctgcctcgtc ggtctttttt gcggttttct ttgcaatcat
     2581 tatgcgttca tatcagctcg gaacatatga tcatatgatt tcaaatgtca ttgaatcata
     2641 tacagggcat ctccagatac agcaccctga atatcaaaac aaccctcaaa ttgacaattc
     2701 ttttgacttt aacgattcgc tttttctctt gataagcaac gtagaaaatg ttgtatcagt
     2761 taccccacat cttgaatttt ttgctcttgc ttcaaacgga ccgcaaacta agggtgtagc
     2821 aattctgggt atagatcctg ccagggaaaa gacattttca aatccggaaa ataaacttgt
     2881 caggtacagg ataacagacg aagctctttt agaaatgacc caaagtggta cttttcctgc
     2941 atcggttctt aaaaaattgc aggaaaacaa ggaccggtcg tattcatctc gtgcccgcct
     3001 tgaacttgaa ctcggactgg caattactga gactgaaaag tatattcctg aaattataag
     3061 attctgcgaa gtacaaaatg gattccttaa tgaagaggat gatggaattc ttgtttctga
     3121 caggcttgcc aggtatctta aagctgaaat tggtgataca ataatactta tcggtcaggg
     3181 atatcaggga gcctccgctg caggtctttt cccagttaga ggtattatca aaatgcccgc
     3241 tcccgacctt gataacaggt taataatgat gactatacct gccgctcaga aatttcttga
     3301 cgctccaggt aaaataacat ctgtttccat taatcttact gacaggtcaa cacgtactat
     3361 taaaaaagcc cttgacagaa taaataagct tatggaaggt atgggtacaa ctgtaaaaac
     3421 ctggtatgaa cttaatcctg tactttacca gcagatacag ggcgacagtc agagtggcat
     3481 ggtaatgctt ggagtcctct acttcattat cttctttgga atattcggca ccgttataat
     3541 gatggttgct gaaagaaaaa aagaatttgg cgttctcatc tcaattggca tgcagaaaac
     3601 caggctcaaa agaataatca gcatggaaat ggcaatgctc ggtttacttg gattggcagg
     3661 aggtatgtta gctgctgtac catttattct ttacttcttc tataatccca ttgtactgaa
     3721 aggagacctt ggaaaaatga tggaagatat gggatgggat gctataatgc ctgcaaaatg
     3781 gttcggacct tattattaca ttcaagcttt aatcgtcgta attatgattt tacttgcaac
     3841 tctgtaccca ttacgtaaaa ttaacaagct taaagaagtt gaagctttaa gaagctgaaa
     3901 aacaaaaaat aatatgatac cggaaatttc atggaaaaat atatggcgta ataaaacacg
     3961 cagtttaata gtaatcactg ctgttaccct tggaataatc tcaggagtat tactggtcgg
     4021 aattatgcag ggatgggttg aacaaagaat ccacgatgca atccacaacg aagtctcaca
     4081 tgttcagatt cataacgcag aatactacga taatgaagat attttaagca ctattgatag
     4141 cctgaataaa ataatctctg ttctggagtc tgaccctgag gtaaaagcat ggaccgaaag
     4201 aacaaagctt actgctattg cgaccacacc atgggcaagt acaggattat tcatttatgg
     4261 cataaatccg caaaaggaga aagaagtgtc agaaatctat actaaaatcg tacccaatgg
     4321 gggtgaatat cccgacataa ataagaccgg atatatcctc ataagcgata aaacagcaga
     4381 gatacttcag ctcaaacaat atattattac agaaccatta atagaaaaat tcagagaatc
     4441 cagattgcct gacaatgtcg tcataaaact tgattcaatt aaaggtatac ggttcagatc
     4501 gcctaaggaa tttaaagcaa ttctcgaaaa aaaactgaac cggaaagagc tggacatgta
     4561 tggaaaattg attatggaca gtgcacttga ctacagaata agaaacagaa tacagataac
     4621 tctttccgat tgcaacggca atccggtgca gggaactttc agagtatgtg gcatttacaa
     4681 gacaacaaat accggatttg accaggcttc tgcttttgtg aacatcaatg aactggcgga
     4741 gctatatggt tcagacacac cgcttgtgca tgaaatagca attatgctcc ataatgctga
     4801 aaatattgac aatgtaaaaa acaaactttc aacagtttct gataaaaata tcgtacacac
     4861 ctggaaagaa ctcgcaccag atgcagctat gatgaatgat tttatgtata tgtattactt
     4921 tatttttata ggcatcataa tgctggcact agcctttggc attataaata caatgcttat
     4981 ggcaattctc gagcgaacta aggagcttgg aatgcttatg gctataggaa tgaacaggaa
     5041 aagaattttc agcatgataa tgcttgaaac aattttcctg acttcagcag gggctgttgc
     5101 tggaatgctt gccggctggg ttcttgtatc catgctggga aagacaggaa tacatttttc
     5161 aagctgggga gaaggctttg aagctatagg ttattctgcc aatgtttatc ctgttgtcac
     5221 atttgatttc ttcattttcg tcatagcaat ggttgttttt acagcagttc tctcatcaat
     5281 ctggccagca cgcaaagcac ttaaacttaa ccccgttgaa acactcagaa cagaataaat
     5341 aaacattaac gatatgaaaa tactcgaact gaaaaaagtc aataagatat atgatggatc
     5401 ggaagttcct gttcatgctg tcagagatgt tacgcttgaa tttgaggaag gcgaatttgc
     5461 cgcaatagtg gggccttcag gctcaggaaa aaccactctt ctaaacctga ttggaggact
     5521 cgatatgccc acctcaggag aaataataat agatggggta aatcttgcaa agcttaaacc
     5581 atctcagctt atcgatttca ggctcagaaa cattggcttt gtctttcagt cctataatct
     5641 gatcccggtg cttaccgcca gagaaaatgt tgaattcatt atgtcgttgc aaaaatggcc
     5701 cagaaaagaa cgagatgccc gaacttatga attgcttaaa gctgttggac ttgaggaaag
     5761 aatgaacagt cgaccttcaa aactttcagg tggtcagcaa caaagagtag cagttgcccg
     5821 ggcacttgct cccaaaccca aattcattct tgccgatgaa ccaacagcaa acctcgactc
     5881 aaaagctgcc tccaatcttc tcgaaataat ggaaaaacta aacagggaag aaaaaataac
     5941 ctttatcttc tccacacatg atccaagagt agtgaaaaaa gcacgaaggg taataacact
     6001 tgaggatggt cgtgtttttt ctgatgaaaa aatacaaagt gtaaacgatt gacaaaatga
     6061 ttaaacctcc tgtactgtca ataattgcag taattattgc atttaatgta aataatattg
     6121 caggtcagga aaaagagaag cctgtcactt tgaagggtta tgttacaaca ctccagaatg
     6181 caatgttcga ttcactctca ggtccggtgt catataataa tatccttcac aacaggctta
     6241 atttcaaagg ctatattaac aacaacatta tccttggaat cgagcttcgt aacagattat
     6301 ttacaggtga tctagcccga actggaaact tttattctga tataaccgga agggataacg
     6361 gatggtttga tatgtcgtgg aacgttatta aagaaaattc gttttttctg aatacaacta
     6421 tcgacaggct ctggattgct atgaaactgg gaaaactgga aatcacaacc ggaaggcaga
     6481 gaattaactg ggggcaggca attgtctgga atccaaacga cattttcaat gcatactcgt
     6541 tttttgattt cgactacgtt gagcggcctg gtagtgatgc gctaaggttc cagtatttta
     6601 cttcctcatc atcaatagtt gaactggcag taaaagcaga tagtgaagac cgggtcaccg
     6661 ctgcaggact tatcagattc aaccgatggg gatacgatat tcagtttctg ggaggttatt
     6721 caaacagttc agatattgtt gcaggagctg gctggtcggg tacactttta aatacttctt
     6781 tcaggggaga aatctcatgg ttctatcctg taaataaggg ggagaaaagc actcttatag
     6841 caactgccgg aattgataaa atattttatg acaattctgt gttgcagatt gaattaatgt
     6901 actcaggaaa ccccgtggcg ctaaacgata tcaatatact ctattctggt actctttccg
     6961 caaaagacct tgcattctca aaatttactg cattcggcag tttcacctgg gcattaacac
     7021 ccttgctgaa taccggaatt tcagcaatgt ggtttcctga tcttaaagga tattttgctg
     7081 gtccatcact tgattattct atgtcagaaa atatagatct ctcggtcatc tggcaacact
     7141 tcaacatcca tactggaagt ataaagactc gaatgaacct tgctttcctg aggttgaaat
     7201 atagtttcta ggaattttat acttttgcca tgcttaaaat tgtgtatgtc ccttgttcat
     7261 gaatttgaaa aaagcggtaa ctggcttttc agaagaagaa gctggttgcc tgtatttata
     7321 gcaatacctg gattcttaat actatatttc actgaccatc agacgattac ttttaaatgg
     7381 gaaatcgagt tgtgcaactt tttttctgta aaatcattta agccacgatt aattttttga
     7441 ca
//
LOCUS       DAOSNB010000104         7432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_9977_length_7432_cov_4.654873, whole genome shotgun sequence.
ACCESSION   DAOSNB010000104 DAOSNB010000000
VERSION     DAOSNB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9977_length_7432_cov_4.654873"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLG42_05605"
     CDS             complement(<1..977)
                     /locus_tag="PLG42_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30717.1"
                     /translation="MKSPTLKILALFIISVLSRNGFSQKTNISLLKEHPIFYEDPSIK
                     EWASACRIIRGYIQIDKKELGRASAGEETACLGKADNVTVSLGDSGIAVVTFSEPVRN
                     IDGYDFAVFENSFDGQFLELAFVEVSSDSMRWVRFPSISLTPTNIQTGTFGFTDAKNI
                     YNLAGKYRLFYGTPFDLEELKDSAGIDLSSIKYIRIIDAIGCIDDRYASYDALGNKIN
                     DPWPTPFPQSGFDLDAVAVLKQVTEINIPEASVPFKIYPNPSTGKIYIDLPGGAMHDV
                     FISDNTGKVLSKKRIGDYVNTFNIENFKPGIYFIQIIGNNTRYVTRIVI"
     gene            complement(977..1717)
                     /locus_tag="PLG42_05610"
     CDS             complement(977..1717)
                     /locus_tag="PLG42_05610"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOU30718.1"
                     /translation="MNARFFLFATLLISAVVIISCQKEKVETDIVDFEELPLDDSGVW
                     NGSDGSGGFRSGNIFFVNHYNSLYQAWSGFAYTNHSDTVTGDYTNQYSSITGSGFDNS
                     LKYGVFYFSGIPDTIVFEIPEKVTDIALCNTTFTYKAVKNGTVFSKKFGGDSGNDPDW
                     LKVTLTPISEEGIALGYVDVYLADFRYTDNKYDYISNVWISIDLSQFGFIKRLKFEMS
                     SSDSGEWGMNTPAYVCIDNIKGILAKDK"
     regulatory      complement(1826..2030)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2107..4287)
                     /locus_tag="PLG42_05615"
     CDS             complement(2107..4287)
                     /locus_tag="PLG42_05615"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOU30719.1"
                     /translation="MRVKFLALLCLLSIIAGGCREEHLISSRDYRKKVENDFKERRKL
                     AAGRKEELFAVFRSGLDLPVAEALKFLYAYMPLSDLADYDGEFFYDNVKIALKARNET
                     PWGKSIPEDLFLHYVLPVRVNNENLDSFRIVYYDEIMSRIKGLDAESAALEINHWCHE
                     RVAYQPSDIRTSGPLSTILSARGRCGEESTFTVSALRSVCLPARQVYVPRWAHTDDNH
                     AWVEVWINGNWHYMGACEPEPVLDRGWFTEPARRAMLVHTKSFGAPSNEEKSVLEFRN
                     YSIINNLSMYADTRIVNVKLTDSFGKPVEDASVEFCIYNFSEFYPLIVLSTDKNGTAS
                     FETGYGDFLVWAHKGETFDFSFVKAFDKDTVFLCPIKNPSDSGIIHIDINPPPVKKPF
                     PPLPEQLVHENDKRLAYEDSIRNNYIKSWLSENEAVSLAASLNIPAEEVVDIIRKSEG
                     NYREIIRFLLEFKDIPTKKKLKILNLVSEKDLRDTRAAVLADHLQNAIPYDSSLMWYD
                     RDTYDEYVLNPRIADEMLRSWRSYLKDKIPDKLRHEALRNPEIIIQYVNDNIIIKEEE
                     NYYSTPLSPSGTHDLGVADNHSRNIYFVALCRLSGIPARLDKGTMRPQYYFNRKWNDV
                     YFAGENPPGTKGFLKLVCEQSDLVPEYFKNFTIARFENGRYNTLQFDYDMKISDFEDK
                     IPLPPGYYMVVTGNRTLNDKILASMKFFRINPVETTRVEVVVRK"
     gene            complement(4303..5340)
                     /locus_tag="PLG42_05620"
     CDS             complement(4303..5340)
                     /locus_tag="PLG42_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU30720.1"
                     /translation="MGKKVKNKDLAIRLGVSPTLVSLVLNNKADLYGIKKETQEKVLA
                     LARQMGFFDVKEEKRAPYPVETAPGIVGMIVPSLADQFVYDITPYLQKEFSSIGLGFT
                     VILKDPDDGRFNRFVSAFRKFFSGLILVGEAADEKTISTLRDNDYPFVLLEKTTEKYR
                     LNTVMTDVMAGINMLTEYIGRKGYRNILILCSEKRHREKEKIIKELVNSLNRIPGGIS
                     RPLIEFVNEPVAEEDFDFKQIWKYLQPPLSIQLIITINASLVYPLLSALENKKIRVPY
                     DVALISFEEGPGFAFLHPPITSLRKPVSALAHKASNMIWSEIKNAGKSKYRRQLQLAP
                     ELVIRRSCGNF"
     gene            complement(5512..>7432)
                     /gene="yidC"
                     /locus_tag="PLG42_05625"
     CDS             complement(5512..>7432)
                     /gene="yidC"
                     /locus_tag="PLG42_05625"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOU30721.1"
                     /translation="IGFSIYNNARLNKIYNEKVTLADSLYKAGSLEKARTEYINALNI
                     KPNQPDIILKLNDINLQLGLVQPDSLKNIVEQKEPEKVPAADLSEPAEGGTASYGIFS
                     GSASGANDFITLENNLLELKISLKGGRIYSARLKEYRTYDSLPLILFDGDSTIFGFNF
                     FTADNKPVQTNDLFFKFPSEERHFIVTDKPQSVKLYLYADSNKYIEYTYTLSPGKYTV
                     DFNASFVSMSGVVAQNQNTITLDWRMYIPQKEKSRQSEESYTTIKYKYYQDEVDGIKE
                     RSRNEIEKVDIPTRLSWVAFKDQFFSSVIITDHFFQNGTFSSTRLPASEKYIKYFTAE
                     IGVPFVPSNPEPLQFKFYLGPNHFSTLKKEGYELEQIVTLGKNITRWINQYLIIPIFN
                     WLNRYISNYGIIILILTIIIKIILFPLTFKSYQAQAKMMVLKPLVDEIAKKYPKQEDA
                     MKRQQATMELYKRAGINPLGGCLPTLLQFPILYAMFRFFPTSIELRQEPFLWAKDLST
                     YDSILNLPFTIPLYGDHVSLFTLLMTASTIITMKMSGSTSGQDQPGMKVMMYMMPVMF
                     MLILNNFSAGLTYYYFLANVLSYIQNLVSKRFINQEEILAKIEESKKKPLKKSKWQQR
                     LEEAAKARGINPPKK"
BASE COUNT         2210 a         1467 c         1342 g         2413 t
ORIGIN      
        1 ttgattacaa ttcgtgtaac atatctggta ttattaccga ttatctgaat aaagtaaatg
       61 ccaggtttaa agttttcaat attgaaagta ttgacgtaat cacctatgcg ctttttgcta
      121 agaaccttac ctgtattatc tgatataaat acatcatgca tagctccccc cggtaaatca
      181 atatatattt ttcctgttga aggattagga taaattttaa aaggcactga agcttcggga
      241 atgttaattt ctgttacctg tttaagtaca gcaacagcat caagatcaaa tcctgattgc
      301 ggaaatgggg ttggccaggg gtcattaatt ttgttgccaa gggcatcata tgaagcatat
      361 ctgtcatcaa tacaacctat agcatcaata attctaatat acttaatgct tgataaatct
      421 atgcctgctg aatctttaag ttcctcaagg tcaaagggag tcccataaaa taacctgtat
      481 tttcctgcaa gattataaat attcttcgca tcagtaaaac caaaagttcc tgtttgaata
      541 ttggtgggtg taagtgatat cgatgggaat ctgacccatc tcatactgtc ggaactaact
      601 tccacaaaag cgagttcaag gaactgacca tcaaaggaat tttcgaatac tgcaaagtca
      661 tacccgtcaa tatttctaac cggttcactg aaagtaacaa ctgcaatacc agaatcgccc
      721 aggctcacag ttacattatc tgccttaccg agacaggcag tttcttcgcc tgctgatgcc
      781 ctgcccagtt cctttttatc aatctgtata taccctctta tgatcctgca agcagaagcc
      841 cattctttta tagaaggatc ctcgtaaaat attgggtgtt cctttaatag agagatattg
      901 gttttttgag aaaatccatt cctgctaaga acagaaataa taaacagagc caatattttc
      961 aaggttggcg acttcattat ttatcctttg caagaatacc tttaatgtta tcaatgcaga
     1021 catatgccgg ggtgttcatt ccccattctc ccgagtcgct tgatgacatt tcaaatttca
     1081 gccgcttgat aaatccgaac tgactgaggt ctatagatat ccatacattc gatatataat
     1141 cgtatttatt atcggtgtat ctgaaatcag caagataaac atcaacataa cccagggcaa
     1201 taccttcttc actgattgga gtaagtgtaa ctttcagcca atcagggtcg ttgcccgaat
     1261 cacctccaaa ctttttgctg aaaaccgttc cattttttac agctttgtaa gtaaaagttg
     1321 tgttacagag ggctatatct gtaacttttt caggtatttc aaatactatt gtgtcgggta
     1381 tgcctgaaaa gtaaaaaacg ccgtacttca atgagttatc aaagcctgac cctgttattg
     1441 aactgtactg attggtataa tcacctgtta cagtatcgct atgattagta taagcaaatc
     1501 cgctccatgc ctggtacaga ctgttgtaat gatttacgaa aaaaatattt cccgatctga
     1561 atcctccgga gccatctgaa ccgttccata cacctgaatc atccagaggc agttcctcaa
     1621 agtccactat gtcagtttca actttctctt tctgacatga tataattaca actgctgaaa
     1681 taagaagtgt ggcgaataaa aaaaaccttg cattcataag aatatattta agattaacat
     1741 caaaatctca tcaatattct taactgccag gaaattaagt tggaatcatt cttgatgatt
     1801 cttcgctttt cttcccgaaa gcttagaact attgaacttg tatggcaggt cttctgactt
     1861 gtcccggttt ttgaagcctt cccatcccgg tttatcagga cagtggcagg ttatcaaaaa
     1921 ccatgatcgc tttgcgacca ggcatcacag cagcgggcac tgttacggat tctaaccgta
     1981 ttccctttta ttgcatatta aaatgcaata ccatacaaac acaaatatag gatttttgaa
     2041 ctatgactcc tgctttaaaa ttaagaattt ttataaacaa ctgttaatta attgaaaata
     2101 aaagttttat tttctgacaa caacttctac tctggtcgtt tcaacaggat ttattctgaa
     2161 aaatttcata gatgcaagaa ttttgtcatt caaggttctg ttgcctgtta caaccatata
     2221 gtatccggga ggtaatggaa ttttatcttc aaaatcactg atcttcatat cataatcgaa
     2281 ttgaagagta ttatatcttc cattttcaaa ccttgctatt gtaaagtttt tgaaatattc
     2341 aggaaccaaa tcagactgct cacaaacaag tttcagaaat ccttttgtcc cgggagggtt
     2401 ctctcctgca aaatatacat cgttccactt tctgttaaaa taatattgcg gacgcattgt
     2461 ccctttgtca agccttgcgg gaataccact gagacggcaa agagcaacga agtaaatatt
     2521 tcgcgaatga ttgtcagcaa ctcccaaatc atgagtacca gatggagata atggtgtact
     2581 gtaataattt tcttcctctt taataattat attatcattc acatactgta taattatttc
     2641 aggattccgc aatgcttcat ggcggagttt atccggaatt ttatccttaa gatagcttct
     2701 ccagcttcgg agcatttcat ctgctatcct cggattaagt acatactcat cgtaagtatc
     2761 tctgtcatac cacattaaag agctgtcata tggaattgca ttttgaagat gatcagcaag
     2821 aactgcagcc cttgtatctc tgagatcttt ttctgaaaca aggttcagaa ttttaagttt
     2881 tttcttcgtc ggaatatctt taaattctaa tagaaatctg ataatctctc tatagttgcc
     2941 ctcagatttt cttataatgt ctactacttc ttcagcagga atgttaagtg aagcagcaag
     3001 tgaaacggct tcgttttcac ttagccacga tttaatatag ttgttcctta tgctatcctc
     3061 gtatgccagt ctcttatcgt tttcatgtac aagttgctca ggtagtggtg gaaacggttt
     3121 ttttacagga ggagggttga tgtcaatatg aataatgcct gagtctgatg gattctttat
     3181 gggacaaaga aacactgtat ctttatcaaa ggcttttaca aaactgaaat cgaaagtttc
     3241 tcctttatgg gcccatacaa gaaaatctcc atatcctgtt tcaaaactgg cagtgccatt
     3301 tttgtctgtt gagagtacga tcagagggta gaattcagaa aaattataaa tacaaaattc
     3361 aacggaagca tcctcaacag gctttccaaa agaatcagtc aatttaacgt taactattct
     3421 tgtatcagca tacattgaaa ggttattaat aatggaatag tttctgaatt caagaacaga
     3481 tttttcttca tttgaaggag ctccgaatga ttttgtatgt acgagcattg cccgccttgc
     3541 tggttcggta aaccagcctc tgtcgagcac gggttccggt tcacatgctc ccatataatg
     3601 ccaattgcca tttatccata cttccaccca tgcatgatta tcatcggtat gagcccatct
     3661 cggaacgtaa acctggcgtg caggcaggca tacagacctg agggcagaga ctgtaaaggt
     3721 cgattcttca ccacaccgtc ctcttgctga aagtatggta cttaacggac cggaagtccg
     3781 gatatccgag ggctggtatg ctactctttc atggcaccag tgattaattt cgagagctgc
     3841 tgattcagca tccagccctt ttatcctgct cattatctca tcataataaa ctattctgaa
     3901 tgaatcgagg ttttcattgt ttacccggac aggaagcaca taatgcagaa ataagtcttc
     3961 tggtatggat tttccccatg gagtttcgtt tcttgctttc agggcaatct tcacgttgtc
     4021 atagaaaaat tcaccatcgt aatcggccag atctgaaaga ggcatatagg cataaagaaa
     4081 ttttagtgct tcggctacag gcaaatcaag acctgacctg aaaacagcaa aaagctcttc
     4141 ttttctgcca gcagcaagtt tcctacgttc tttaaaatcg ttttctactt tttttctgta
     4201 gtcacgtgat gaaataagat gttcttccct gcatccaccc gcaattattg acaacagaca
     4261 gagcagggct aagaatttaa ctcgcataat ataatgattc gattaaaaat ttccgcagga
     4321 cctccttatt acaagttccg gagcgagctg taactgtctt ctgtacttac tctttccagc
     4381 atttttaatc tccgaccaga tcatattaga agctttatgc gcaagtgcgg agacaggttt
     4441 tctcaaagaa gtaatcggag gatggaggaa ggcaaatccg ggtccttcct caaaagatat
     4501 gagtgccaca tcatacggta cccttatttt tttgttttcg agtgctgaaa gcagaggata
     4561 gacaagggat gcgtttattg ttataataag ctgaatactg agaggtggtt gcaagtattt
     4621 ccaaatctgt ttaaaatcaa aatcctcttc tgcaacaggc tcatttacaa attcaatgag
     4681 tggccggcta atgcctccag gaattctatt cagggaatta actaattctt taattatctt
     4741 ttccttctcc ctgtgtcttt tctcactgca aagtattagt atattcctgt aacctttcct
     4801 gcctatatat tcagtcagca tatttattcc tgccatgaca tctgtcataa ctgtattcag
     4861 cctgtacttt tcagttgttt tttcaagcag gacgaatgga taatcattgt ctcttaaagt
     4921 gctgatagtt ttttcatcgg ctgcttcacc aaccaaaatc aacccgctga aaaattttct
     4981 gaaagccgaa acaaatctat tgaatcggcc atcgtcagga tcttttagaa taacagtaaa
     5041 tcctaaacca atgctcgaaa attccttctg cagataaggc gtgatgtcat acacaaactg
     5101 atcggccagt gaaggaacaa tcattccaac tatcccggga gcagtctcca ccggatacgg
     5161 agctctcttt tcttccttaa catcaaagaa acccatctgc ctggcaagtg ccagaacttt
     5221 ttcctgggtc tcctttttta taccgtaaag gtcagcttta ttgttaagta ctaatgatac
     5281 cagagtagga gatacaccta atctgatagc cagatctttg tttttaactt ttttccccat
     5341 tcccctttta ttagaaattt aggtttaaaa ataagaatta tttaaattct catacaaaat
     5401 tttttaaaga atattaataa ttgaacagaa ttatatttgt cggctaaaga gttttcgaca
     5461 aaatgaatta tctggtctaa ttttaaaaac caataattgt ttagcgatag tttatttttt
     5521 aggtggattt attcctctgg cttttgcagc ttcttcaagt cgctgttgcc attttgattt
     5581 tttaaggggt tttttcttgc tttcttcaat ctttgccagt atctcttcct ggttaatgaa
     5641 acgttttgag acaagattct ggatatagga aagaacatta gccaggaaat aataatatgt
     5701 aagccctgct gaaaaattgt ttagtatcag catgaacatt accggcatca tgtacatcat
     5761 tactttcatt ccaggctggt cctgacctga cgtagagcct gacattttca tcgtaataat
     5821 ggttgacgcc gtcatgagca aagtaaagag gctcacatga tcgccgtaga gaggaatagt
     5881 aaaaggcagg ttgagaatag aatcgtatgt tgatagatct tttgcccaca ggaatggttc
     5941 ctgacgaagt tcgattgatg tgggaaagaa acggaacatt gcatacaata tagggaactg
     6001 caggagtgta ggaaggcaac ctccaagcgg attaatccct gcccttttgt aaagctccat
     6061 tgtggcctgc tgccgtttca tggcatcctc ctgtttcgga tatttctttg ctatctcatc
     6121 aacaagaggt ttcaatacca tcattttcgc ctgtgcctgg tacgatttga aagtaagcgg
     6181 gaagagaatt atcttgataa taatagttaa tattaaaatg atgattccat agttcgaaat
     6241 gtacctgttc agccagttaa aaatcgggat aatgaggtat tgatttatcc accgtgtaat
     6301 atttttgccc agtgtaacta tctgctcaag ttcgtaaccc tcttttttaa gagtagaaaa
     6361 gtgattcggc cccaggtaga atttaaattg aagaggttca gggttcgaag gaacaaacgg
     6421 cactccaatt tcagcagtga aatattttat gtatttctct gatgcaggaa gacgggttga
     6481 tgaaaatgtg ccattctgga aaaaatgatc tgtgataata acagatgaaa agaactgatc
     6541 cttaaatgcc acccaactta accttgtagg tatatcaact ttttcaattt catttctcga
     6601 cctttctttt ataccatcta cttcatcctg ataatatttg tatttgatgg tagtatatga
     6661 ttcttcactt tgcctgctct tctctttttg cggaatatac attcgccaat cgagagttat
     6721 tgtattttgg ttttgcgcaa ctaccccgga cattgacaca aagctggcat taaagtcgac
     6781 ggtatatttt cccggggaaa gagtatatgt atactctatg tatttattac tatcggcgta
     6841 caggtaaagc tttacagact gaggcttgtc tgtaacaata aaatgccttt cttccgaagg
     6901 aaatttaaag aaaaggtcgt tagtctgaac aggtttatta tcggctgtaa agaaattaaa
     6961 accaaatatg gttgagtcgc catcgaatag tataaggggt aatgaatcat aagtcctgta
     7021 ttctttcaat cttgctgaat aaattcttcc acctttaagt gaaattttca attcaaggag
     7081 gttgttttcg agagttataa aatcgttagc tcctgaagct gaaccagaaa aaattccgta
     7141 cgaagccgtt cctccttcag ccggctcaga taaatctgct gctggcactt tctcaggttc
     7201 cttttgttcg acaatattct ttagactatc aggctgaaca agacccaatt gcagattaat
     7261 atcgttaagt ttaaggatta tatcaggctg atttggctta atattgagtg cattaatata
     7321 ttcagttcgt gctttttcca aactccctgc cttatataat gaatcagcaa gtgtaacttt
     7381 ttcattataa attttattaa gccttgcgtt attataaata ctgaatccga ta
//
LOCUS       DAOSNB010000105         7338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10150_length_7338_cov_4.438144, whole genome shotgun sequence.
ACCESSION   DAOSNB010000105 DAOSNB010000000
VERSION     DAOSNB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10150_length_7338_cov_4.438144"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLG42_05630"
     CDS             complement(<1..1302)
                     /locus_tag="PLG42_05630"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU30722.1"
                     /translation="MRILKCFAGVNLVLLILAILFFLPGCGESKKGINKEVEENFINP
                     PSSSRPRVWWHWMNGNVTKDGIRADLEWMHRVGIVGFQNFDAGLSTPQIVEKRLIFMT
                     PEWKDAFRYTTRLADSLGLEMAIAGSPGWSESGGPWVKPSEAMKKVVWSETVIEGGKP
                     FNGTLPHPPATTGRFQNLGSERGFSLEEEGRERPEFYADIAVLAYRLPQADVSLKELN
                     PKVTSSGGKFTLEMLTDGDLATGSLLPVNKSDSVAWIKFEFDKPQTIYSVSMVGGGSP
                     GRFGFGRSRQRRALEASDDGKTFKRITDIPAGGVPQNTITFKPVTAKYFRVTITNPPS
                     TDDMAQLGQMLGIRNRQGEQQPRGTEIAEIVLYTVPQIHRFEEKAGFAVVTNVDKILT
                     PELMPESAVKHADIINITDKMKEDEPSNGYHRKVNGEYSESA"
     gene            1544..2167
                     /locus_tag="PLG42_05635"
     CDS             1544..2167
                     /locus_tag="PLG42_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30723.1"
                     /translation="MVYNKHKGNNNEANRYFNYLFISIILVFIAGIVTYGEKFRLWEY
                     AYSYIGMFRTPGGNPNTTSFIIFIAGSLFNSFICFKISSLYEEKLYRLLFGICGTGFL
                     LLMLPCDILNQVHSAGGAMVFGSLWIFSLTGIYVIYHSGKSQKALTYFVLLNITILPY
                     AYLYFINSPLQVIAQKPAIIGLIFIVKMIITEHAGKHVGEDLKREAA"
     gene            2332..3822
                     /locus_tag="PLG42_05640"
     CDS             2332..3822
                     /locus_tag="PLG42_05640"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOU30724.1"
                     /translation="MSKSPDGSTEQRFTTRIGLIMSALGIAVGTGNIWRFPRIAAQNT
                     GDTGAGAFLIAWVIFLFLWSIPLIIAEYASGRKHRSGVIGVFIKGMGERFAWIGSFVA
                     FVPLAITFYYSVVVGWCAYYFIYMATHSLPSTFESAMSIWNSYQSGYWPLITHLGSVG
                     LGALAIWKGVNSIEKVNKVLIPTLLLIVIICVIRAITLPGAGTGLKYLFSADLKQLGN
                     TRTWIDALTQNAWDTGAGWGLFMTYAAYMKKEHGPVGNAFLTGIGNNTVSILAGIMVF
                     GTVFSILRHEAGITDARILEIMRDSGPASTGLTFIWMPQLFNKMFGGGILSVLFFLGL
                     TFAGFSSLIAMLELPTRVLVDAGQKRNRAIILICILVFIFGIPSARSLNFLSNQDFVW
                     GVALMISGALIALMIIKQGANDVRAEINTHESDLRLGRWWNYNIRWFVPSAALVLIIW
                     WLAMSFVPGQWYNPFKEFSFMSCIFQWGIVMILQIIFNKRIARFFT"
     gene            complement(4010..6379)
                     /locus_tag="PLG42_05645"
     CDS             complement(4010..6379)
                     /locus_tag="PLG42_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30725.1"
                     /translation="MPESKALSAPRTMILFDNEWFFHRGDVTGAEKTEFQDSGWKKVN
                     LPHDWSIEDIPGTDSPLDSTAIGGIDAGYFAGGTGWYRKLFYVPASLKDKTFIIIFEG
                     IYMNSDVWLNGKHLGNHPYGYTSFFFDITREITPGKTNVLAVRVKNEGRNSRWYSGSG
                     IYRHIWITIAEKVHFKEWTLAVSTDFIDSKNAMANISAKVINNGYSGENFIAKVRIID
                     PQGNNAGDAKQDINIGSGEGKEVVWQVPVKDPQLWSPEKPALYKAVAEIYEESVDGTG
                     SLVDINTTDFGIRFFRITPEEGFVLNGVPLKLKGGCVHHDNGPLGAAAFDRAEERRVE
                     LLKASGFNAIRCAHNPPSPAFLRACDKLGMLVIDEAFDMWREAKNPDDYHLWFDEWWK
                     KDIESMVMRDRNHPSVIIWSIGNEVPERGKPEGAALAKEMVEYVKNLDPSRPVTAAVN
                     SVTPDKDPYFAALDVCGYNYAVDKYVSDHKRLPERIMMGTESFPLEAFEYWKATIDNP
                     WVIGDFVWTSFDYLGEASIGWLGYPHEGSFYPWHLAFCGDIDICGFKRPQSYYRDALW
                     GDDKTVSMFVKPPVPSFPVNPRKAPWSKWEWHDVVPSWNWDVYEGKPLTVEVYSNCDE
                     VQLTLNGKPVGKKKSSQENKYITTWEVPYEPGVLTATGFDKRGAVATSELRTAGKPAR
                     IRLSADRNSIEAGGQDLSFITVEIVDENGIINPVAENLVMFSVQGPGEILSVGSANPV
                     STESYKKPYRKAFMGKCLAIIRSGKDTGIVKLNAESEGLQGAEVEIKVY"
     gene            complement(6757..>7338)
                     /locus_tag="PLG42_05650"
     CDS             complement(6757..>7338)
                     /locus_tag="PLG42_05650"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOU30726.1"
                     /translation="FGAAKPWENNIVRYCISYNDGLLNEGSVAVWKGGREMTMRNCEI
                     YNNTFLNDTAKGIAVSLVSNCENFKFTNNIFLYNGTFLMENQKIESEKFVSNCLWNKK
                     GKVNLPENILKTNLITDPQINVPEKINFGSIYEIYDGKQIVFSLVPGSPLIDRGEEIL
                     PPFGLPFPGVDIAGTKVPQGSRPDIGAIEFIKR"
BASE COUNT         1976 a         1643 c         1447 g         2272 t
ORIGIN      
        1 agccgattct gagtattctc catttacctt ccggtggtat ccattcgagg gttcatcttc
       61 tttcatttta tcagtaatgt ttatgatgtc tgcatgtttc actgcgctct caggcattaa
      121 ttcaggagta aggattttat ccacgtttgt aaccactgca aagcctgctt tctcctcgaa
      181 gcgatgtatt tgcggtactg tataaagtac tatctcggca atttcggtac ctctgggttg
      241 ttgttcgccc tgtctgtttc tgatgcccag catctgtcca agctgcgcca tatcgtctgt
      301 cgaaggcggg ttggttatgg ttactctgaa atattttgct gtgaccggct tgaaagtaat
      361 ggtattctga gggacgcctc ccgcagggat atctgtaatt cttttgaatg tcttgccgtc
      421 gtcgctggct tcgagtgcac ggcgctgtcg gctgcgtcca aatccgaacc tgcccgggct
      481 tcctccgcct accatcgaaa cagagtaaat agtctgcggc ttatcaaact cgaactttat
      541 ccatgccaca ctatctgatt tattcaccgg gagcaaggaa cctgttgcca ggtcaccatc
      601 ggtaagcatt tcaagagtga atttaccgcc gctcgatgtt accttcgggt tcagttcctt
      661 cagggaaaca tcagcctgtg gaagcctgta tgccagaacg gcaatatcgg catagaattc
      721 aggtctctct cttccttctt cctcaagaga gaagcctcgt tcagaaccaa gattctgaaa
      781 cctgccggtt gtagccggag gatgaggcag agtgccattg aatggtttac ctccttcgat
      841 gacggtttcg ctccacacca cttttttcat tgcttcggag ggtttaaccc acggacctcc
      901 gctttcactc cagccgggtg aaccggcaat agccatctca agaccaaggg agtcagccaa
      961 ccgggttgta tacctgaagg catccttcca ttcaggggtc atgaaaataa gacgcttctc
     1021 aacaatctga ggcgttgaaa gccccgcatc aaaattctgg aagcccacaa taccaacacg
     1081 gtgcatccac tcaaggtcgg cacgaatacc gtccttcgtc acattgccat tcatccagtg
     1141 ccaccaaaca cgtggccttg aacttgatgg aggattaata aaattttcct ctacctcctt
     1201 gttaattcct tttttcgatt ctccgcaacc agggagaaaa aaaagaatag ccagaattaa
     1261 tagtactaaa ttaaccccgg caaaacattt taaaattctc atttttaaat agtttatata
     1321 attgaatatc aatatatttt caatttaaaa ttataacaat gctcatcaat ttaacctttc
     1381 acttcttaac acctgttaat tgcatctgtt tacaagtttc aaactacagc ctgtgataac
     1441 gcaatttaaa aaataaaaat tacaattata aaaacagttg agcctttgag agaattttat
     1501 taacttataa ggtcaaattg aatatgtcag atataccgca aaaatggttt acaataaaca
     1561 taaaggaaac aacaatgaag caaacagata ctttaattac ctttttatca gtataattct
     1621 tgtatttatt gcaggcatag ttacatatgg cgaaaaattc agattatggg aatatgcata
     1681 cagttatatt ggaatgtttc gtacaccggg tggaaatcca aatactacct catttatcat
     1741 ttttattgcc ggaagtctgt tcaactcgtt tatatgtttt aagataagca gcctctatga
     1801 agaaaagctg taccgtttgc tttttggcat ttgcggaacc ggatttcttc ttcttatgct
     1861 accctgtgac atccttaatc aggttcattc tgcaggcggt gcaatggttt ttggttcact
     1921 ctggattttt tcactgacgg ggatttatgt aatatatcat tccggcaaaa gccagaaggc
     1981 attgacctat tttgtgctgc taaatattac tatactacca tacgcatatc tctatttcat
     2041 taattcacct ctccaggtga tagcccagaa accggcaata ataggactga tatttattgt
     2101 taagatgatt attactgaac atgccggtaa acatgtgggt gaggatttga aaagagaagc
     2161 tgcataacag acttatttat atttgaacag gagttaaata atttcctttt ccgggcgaaa
     2221 taaagattaa gatttattca atcaggaaga atatttgctt ttccttaaaa ttttcagaaa
     2281 agaaaaattt tatctttaag cgccaaacaa tttcctgtaa aaagatttaa gatgtcaaaa
     2341 tcaccggatg gttcaactga acaaagattt accacacgta taggtttgat aatgagcgct
     2401 cttggaatag ctgttggaac aggcaacatc tggagattcc cgagaatcgc ggcccagaac
     2461 acaggtgata ctggcgcagg tgcatttctg attgcatggg tgatttttct ctttctctgg
     2521 agcattcctc tgattattgc cgaatacgca tcaggcagaa agcatcgttc gggtgtcata
     2581 ggagttttca taaaaggcat gggagaacgg tttgcctgga taggttcatt tgttgcattt
     2641 gtgcctcttg ccattacttt ttattattca gtcgttgtgg gctggtgtgc atattatttc
     2701 atttatatgg ccacccacag tctgcccagc actttcgaat cagcaatgag catctggaac
     2761 agttaccagt cgggttactg gcctttaata acccatctgg gttcagtagg attaggggcg
     2821 cttgccatct ggaaaggtgt aaattcaatc gagaaggtga ataaagtttt gattcccaca
     2881 ctactgctta ttgttattat atgtgtgata cgtgctatta ctctgcctgg ggcgggtaca
     2941 gggctgaagt atcttttcag tgcagacctg aaacaacttg gtaacaccag aacatggatt
     3001 gatgctctta cacagaatgc atgggataca ggggcaggct ggggactttt catgacttat
     3061 gcagcctata tgaagaaaga gcacgggccg gttggtaatg cttttctgac cggtataggg
     3121 aacaacactg tttcgatact tgcgggtatt atggtattcg ggacagtctt ttccatactc
     3181 aggcacgaag cgggtataac cgatgcccgt attcttgaaa ttatgagaga tagcgggccc
     3241 gcatcgaccg gactaacatt tatatggatg cctcagcttt ttaacaagat gttcggagga
     3301 ggtattttat ctgttctgtt ttttctgggt ttgacttttg ctggattttc ctcactgata
     3361 gcaatgctgg agctaccaac cagggtactg gtggatgcag gacagaaaag gaacagggcg
     3421 ataattctta tttgtattct ggtgtttatt tttgggattc cttcggcgcg aagccttaat
     3481 tttctttcga accaggattt tgtgtgggga gttgctttaa tgatttcagg cgctcttata
     3541 gcacttatga ttattaagca gggcgctaat gatgtaaggg ctgaaatcaa cactcatgaa
     3601 agcgatttaa ggcttggcag gtggtggaat tataacatcc gctggttcgt gccttctgca
     3661 gcactggtgc tcatcatatg gtggcttgca atgtcgtttg tcccgggaca atggtataac
     3721 cccttcaaag aattttcatt tatgtcatgc atatttcaat ggggcattgt gatgattctc
     3781 cagataatat tcaacaaacg gattgcaagg ttttttacat gagcatgagt ttatgttgtc
     3841 ttgccctggc aggacaaaaa ttacttaaaa accctcaata gtcctgtaat ggctaaataa
     3901 tcatattgat gggcatcgcc cataaatata gccccaaaaa taccttgcta agcgctgtaa
     3961 gcgcgaaata ccttatcgac tggcatcggt gttacaggac actaaatcat caataaacct
     4021 ttatctctac ctctgctccc tgcagacctt cggattctgc attaagcttt acaatccctg
     4081 tatcctttcc tgatcttatt attgccaggc acttgcccat aaaagccttt cggtaaggct
     4141 tcttatagct ctccgtactc acaggattgg cgcttccaac cgacagaatc tcccctggac
     4201 cctgaacaga aaacatcaca agattctcag caacagggtt tataattcca ttttcatcaa
     4261 caatttccac agtaataaaa ctcagatcct gcccgcctgc ttcgatagaa ttacggtcgg
     4321 cgctaagcct tattctggca ggtttgcctg ccgttctcag ttctgatgtg gcaacagcac
     4381 ctcttttgtc aaaacctgtt gcagtcagta cacccggctc ataaggtacc tcccatgtgg
     4441 taatgtattt attttcctga ctggattttt tctttcctac aggtttgcca ttgagtgtaa
     4501 gctgaacctc atcgcagtta ctatagactt ccactgtaag aggttttcct tcgtatacgt
     4561 cccagttcca ggaaggaacc acatcgtgcc attcccactt gctccacggt gccttacgcg
     4621 ggttaacagg gaaagatgga acaggaggct tcacaaacat tgaaacggtt ttgtcatctc
     4681 cccatagtgc atcacggtaa tatgactgtg gacgtttgaa accacagatg tcaatatcgc
     4741 cgcagaaggc tagatgccat ggatagaagc tgccttcgtg cggatatccc agccacccga
     4801 tacttgcctc gccaagataa tcgaacgatg tccatacaaa atcacctatt acccatggat
     4861 tatcaattgt agctttccag tattcaaatg cctcaagtgg aaatgattct gtccccatca
     4921 tgattcgttc tggaagacgt ttgtgatcac ttacgtattt atctacagca taattatagc
     4981 cacacacgtc aagagcagca aaatagggat ctttatcggg tgtaacggag tttacagcag
     5041 ctgtcacggg ccttgaaggg tcaagatttt tcacatactc aaccatctcc tttgccagtg
     5101 cagctccttc gggtttacct ctctcaggca cctcgttgcc tatactccaa atgattaccg
     5161 aaggatggtt tctgtcgcgc atcaccatgc tttcaatgtc ctttttccac cactcatcga
     5221 accacagatg gtaatcatcg gggttctttg cttcccgcca catatcaaaa gcttcgtcaa
     5281 tcaccagcat tcccagtttg tcgcacgccc tcaggaatgc aggagaaggt ggattatgag
     5341 cacaccgtat agcattgaaa ccgctggctt taagaagctc aacccttctc tcttcggctc
     5401 tgtcgaatgc agcagcacca agcggtccat tgtcatgatg tacacagccg cccttcaatt
     5461 tgagcggaac accgttaaga acaaaaccct cctcaggagt aatcctgaaa aaacgaatac
     5521 caaaatctgt cgtattaata tctaccaatg agcctgtgcc atcaacagat tcttcatata
     5581 tttcagcaac tgccttataa agcgcaggtt tttctggtga ccacaactgt gggtctttta
     5641 caggtacctg ccagacaacc tcctttccct caccgctgcc aatatttata tcctgttttg
     5701 catcacctgc attgtttccc tgagggtcaa taattcgaac ctttgcaata aagttctcac
     5761 ctgaatatcc gttgttaatt actttcgcac ttatattcgc catagcattt tttgagtcta
     5821 taaaatctgt tgacactgcc agagtccatt ccttaaagtg tactttttcg gctatggtaa
     5881 tccagatatg cctgtaaatt cctgaaccgc tgtaccacct gctgttacgc ccttcatttt
     5941 taactcttac tgccagaaca tttgtttttc cgggtgtaat ttcccgggtt atatcaaaga
     6001 agaacgaagt gtaaccgtat ggatgattgc cgagatgctt tccgttaagc cagacatctg
     6061 agttcatgta aataccttca aatatgataa tgaaggtttt atcctttaaa gacgcgggaa
     6121 cataaaacaa tttccggtac cagcctgttc cacctgcaaa atatcctgca tcaatgccgc
     6181 caattgccgt tgaatcgaga ggagagtcag tcccaggtat atcctcaatg ctccagtcgt
     6241 gggggaggtt aacttttttc catcctgaat cctgaaattc tgttttttct gcaccagtga
     6301 catcacctct gtggaaaaac cattcgttat caaaaagtat catggtacga ggcgctgaaa
     6361 gagccttgct ttcaggcaat gaaactgagg gctcttattt gcaaccagcc gaaaatacca
     6421 gaattattac cgagacaata aagaaatatt ttggtgaagt tttcatattt ttaattttat
     6481 tataatattg attaacagat tttttcattt atttcatccc ggccttttat ttagccccca
     6541 tcccagatgg ttttctataa agcctctatt ttgttttcac ccctgaattc ccgttcttac
     6601 taccgcaaag ccggacaggt ctttggggat aacttattaa tatacaatat tttagttttt
     6661 atgtttttat tacacttaca tattacacat ctttttagac cgtcccgttc cgataactat
     6721 tggaagggaa aggtaaataa agcttatgtg aatatattat ctttttataa attctatagc
     6781 tcctatatct ggtctacttc cctgaggtac tttagtcccg gcaatatcaa ctcccgggaa
     6841 tggcaagccg aaaggcggga ggatctcttc tcccctgtcg atcagaggag agcccggcac
     6901 cagcgaaaaa actatctgtt tcccgtcgta tatttcatag atgcttccga aattaatctt
     6961 ctccggcaca tttatctgag gatcagtaat aagattcgtt ttgagaatat tctccggcaa
     7021 attcactttt ccttttttat tccagagaca attagagaca aatttttcac tttctatttt
     7081 ctgattttcc atcaaaaagg ttccattgta tagaaaaata ttatttgtaa acttaaagtt
     7141 ttcgcagtta cttaccagtg atactgctat gcctttcgca gtatcattca aaaaagtatt
     7201 gttatagatt tcacaattcc gcattgtcat ttctctgcca cctttccata ctgccacaga
     7261 gccctcatta agcagtccat cattatagct tatgcagtac ctgacgatgt tattttccca
     7321 tggttttgct gctccgaa
//
LOCUS       DAOSNB010000106         7326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10172_length_7326_cov_4.855453, whole genome shotgun sequence.
ACCESSION   DAOSNB010000106 DAOSNB010000000
VERSION     DAOSNB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10172_length_7326_cov_4.855453"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2688
                     /locus_tag="PLG42_05655"
     CDS             <1..2688
                     /locus_tag="PLG42_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOU30727.1"
                     /translation="SHRYNEAIVHYGKMLEASQKLGIYRNLQMEASVCRKVANCYEMT
                     GKYSDALEYVERAMKLDSAAGYLLGRIEDYRHRGKIYIYMGQYYNGISSLERALELSE
                     GMNQSLKNENRLTIADNYLALGQLYAVMGKTETSLKYTDRALTVFRQAGETRGEMEAY
                     LTLASVYSDQGDLITASRFAENSLKLAERLGLGTARHYHLMATIASGTGDYEKALRYQ
                     EKALEEAKKTRIAAQIIWATVGMGDIYSELGDYRHAGQYYRQAREQRDTIGIMAGSLD
                     ASIGMRLGDVAGASRYYSSEGSVTGNAITSLRMAELLINAGKPDSAFYYLSIAGSAFH
                     SMGNRQGLSNTYLLKGKILNDAGNYSMALAVIDSALRLSEFPDIKWRAWFERGRVYEN
                     SGDDARAVMAYRNAIDIIEKIRGNLTVDEFRSIFLENKREVYDRLIRLLMKNRMPDEA
                     FRISEQARARAFYDILAGKKIDFRGALPGDLVTREQEKRLELQNLYRLLQRSDAEFQG
                     SIAGRASEMPKVMEELTRVQTEYEELLRMLKLNNPAYAEVIAAEPVTIEDVQSELNEK
                     TALISYWVSEDGLSIWLIKPSGTEGFQVKTSYGRLYELIELARKSISSNDDKAANYSL
                     KELYNLLIYPVEKHLEGFRDIVFIPNGPLHFLPFQALKDRNDRYLIDKYNITYSPSAS
                     VYTVCNERPVPGGSKFLGVALSDMPIGGKPGLPGTEDELKNILPLFSESLSATGKNAT
                     ETFVKRNAGSCSILHLATHGTYNYNQPLYSCLLFPATDEDDGRLNVWEVLEMKLNAKL
                     VTLSACETGLGNVTRGDEMTGLSRAFLFAGSPSVVVSLWAVADYPTSFLMSAFYRNLK
                     SYPASEALSLAQRETMKKYPQPLYWAPFVLIGNGNVKAE"
     gene            2986..3573
                     /locus_tag="PLG42_05660"
     CDS             2986..3573
                     /locus_tag="PLG42_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOU30728.1"
                     /translation="MIKFRDIENEEFLVRSLSKGNILAFNTLFKEYSGRLFRFANGYL
                     RSEAEAEEIVQEVFTIIWEKRKDLKDELSFKSYLFTIAFNIIKKHFRRKAYLAEYLNS
                     EIYSDLDISTTDKIDYDSVCDFIRKLVDRLPDRRREIFIKSRFEGLSIKEISEELGIS
                     HKTVENQITAALKFLKENLKREFLPAVLFFFLFIA"
     gene            3767..4807
                     /locus_tag="PLG42_05665"
     CDS             3767..4807
                     /locus_tag="PLG42_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HOU30729.1"
                     /translation="MKEEKVDFDKLKDFFEGRNNDFEYIKKVFCDREKEDELKKVLWR
                     QWYEILKEDIESEKDLSHILYRIHYEINSRKEFIDRRGKIHNILRAFTRIAAVLFIPL
                     LIYSGYQFIRSSGTGIAMPVEITAPAWARVQFTLPDGTRGWLNNRSVLKYEAGINFNR
                     EVILDGEAYFDVAKEKRKQFRVKTDEVIITALGTKFNVASYNNEKNVEVVLEEGSLIL
                     TDKDNRYTCQMKPSELVIYDKTSGHLSKDTVQTNKYTSWKEGRLVFRNDPIDVIARRL
                     ERWYNIEVELKDIFPDDIRLRATFIDEDLYEVLEILKRSLNIDYHINERSLGDDGVFY
                     KKKVILMSKKDI"
     gene            4987..>7326
                     /locus_tag="PLG42_05670"
     CDS             4987..>7326
                     /locus_tag="PLG42_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU30730.1"
                     /translation="MELKISILIILLSVSSVFAGRSYSQTAKISLDIKDKTLEQVMDE
                     IERQSEFYFIFNQKQIDVNRIVDIQANNKRITDVLPLLFEGTDVNYAIIDRKILLTTD
                     PISKDLLKKDLKINLNEEQQQMTVTGVVTDASTGEVIPGVNVVVKGTTIGTITDEKGK
                     YSINVPDRNAILVFSFIGYNPQEILLGGRTLLNVALSLDVKRLEEIVVVGYGVQKKLN
                     LTAAVDQVTSEVITNRPIPNITQGLKGVIPNLNINLLDGKPIQAPSFNIRGTTSIGQG
                     GNALVLIDGVEGDPSLLNPNDIASISVLKDAAAASIYGARGAFGVVLITTKNPEKGRT
                     GVQYSTNYSLKRPIKLPDYVTDGYTYVKMFVEAFLNGDGSFPQNINKTQKVSQAYLDE
                     FKRRVESGQPYNTVEVDPVTGEYVYYASTDWYDLLYKDFTSSIEHNLAISGSSDKAAY
                     LVSGRIMGQNGLFRYNTDDYAMQNLRAKGSVNIFKWLEVTNNSDYSHMSYHNPMNVGE
                     GSGIWRNIADEGHPSAPLFNPDGTFSMSAAYTVGDFVYGKNGIDTKRTVIKNTTGATA
                     SFFDNKLRIKGDFTFQNTRYDRIRKRVQVPYSNKPGVIAYVGTKTNDLTYEDRKTDYL
                     ATNIYSEYENNFGNNHYLKFMIGYNFEQSTYNRLYVLRNGIIYPDAKDINLCLGQSIE
                     TSGGYEKWIVLGGFSRLNYSFKDRYLIEINARYDGSSKFPSNQRFAFFPSISAGWRVS
                     KESFFKVSPKLVNDLKIRASYGSLGNGNIASYVYQEQFSI"
BASE COUNT         2405 a         1154 c         1632 g         2135 t
ORIGIN      
        1 agtcaccgtt acaatgaagc aattgtacat tatgggaaga tgctcgaagc gagccagaag
       61 cttgggatat accgcaacct tcagatggaa gcctcggtgt gccgcaaagt ggcgaactgt
      121 tatgaaatga caggcaaata ctcagatgca ctggaatatg tggagagggc aatgaaactc
      181 gattctgcag caggttacct tctcggccgt attgaagatt acaggcacag gggtaagatc
      241 tatatctaca tgggacaata ttataacggt atatcatcgc ttgaaagagc tcttgaatta
      301 agtgaaggga tgaaccagag cctgaaaaat gagaacagac ttacaatagc tgacaactat
      361 ctggctctgg gtcaattata tgcggtgatg gggaagacgg agacttcact aaaatatacc
      421 gacagagctc tcactgtatt caggcaggca ggagagacga ggggggaaat ggaagcctat
      481 ctgacacttg cttcagtata ctctgatcag ggagatttga ttactgcatc ccgctttgct
      541 gagaattctc taaaactggc tgaaaggctc gggctgggaa ctgcacggca ttatcatctg
      601 atggcaacaa ttgcgtcggg taccggagat tatgagaagg cattaaggta ccaggagaag
      661 gcacttgaag aggctaaaaa aaccagaatt gctgctcaga taatctgggc aactgtgggc
      721 atgggagata tctatagtga actgggcgac tacaggcatg ccgggcagta ttaccggcag
      781 gcaagggaac agagggatac aatcggcatc atggcaggca gtctggatgc ttcgataggg
      841 atgaggctgg gtgatgttgc aggtgcgagc cgttattatt catccgaagg ttcagttaca
      901 ggtaacgcga taacatcact tcgcatggca gaactattga taaacgccgg gaaacccgac
      961 agtgcttttt attacctcag tatagcaggg tcagcctttc actctatggg taacaggcag
     1021 ggtctgtcaa atacatatct gcttaaaggt aaaatcctta atgatgcagg taattattca
     1081 atggcacttg ctgtaattga ttctgcattg cgtttgtcag agtttcctga tataaagtgg
     1141 agagcatggt tcgaaagggg tcgagtttat gaaaattcag gcgatgatgc gagagccgtt
     1201 atggcatacc gtaatgcgat tgatattatt gaaaagataa gagggaacct tacagttgac
     1261 gaattccgca gtatttttct tgaaaacaag agagaagtat atgacaggct tatcaggctt
     1321 cttatgaaaa acagaatgcc tgatgaggct ttcaggattt cggagcaggc aagagcaaga
     1381 gctttctacg atattcttgc aggtaagaag attgacttcc gtggtgcact tccgggagac
     1441 cttgttactc gtgagcagga gaaaaggctt gaacttcaga atctctacag gttgctccag
     1501 agaagcgacg cagaatttca gggaagcatt gcaggaagag cttctgagat gccaaaagta
     1561 atggaggaac tgacacgtgt gcaaacggaa tatgaagaat tactccggat gcttaaactg
     1621 aataatccgg catatgctga agttatagct gctgagcctg ttactattga ggatgtacaa
     1681 tcagaactaa atgaaaaaac ggcacttata tcatactggg tgagtgaaga tggtctttct
     1741 atatggctga taaaaccgtc agggacggaa ggttttcagg ttaaaacgag ctatggaagg
     1801 ctttatgagc ttattgagct ggcgcgaaaa tcaataagct caaatgatga taaagcagca
     1861 aactacagtc ttaaagaact ttacaatctt cttatttatc cggttgagaa gcatctcgaa
     1921 ggtttcaggg atatagtgtt tatacctaac ggtccgttgc attttctgcc gtttcaggca
     1981 ttgaaagacc gtaatgacag gtatcttatt gataaatata atattacata ctctccatca
     2041 gcaagtgttt atacagtatg caatgagagg ccagtccccg gaggttcaaa gtttctggga
     2101 gttgcacttt cggacatgcc tataggtggt aagccgggtc tgccgggaac tgaggatgag
     2161 ctgaagaata ttctgccgct tttttccgag agtctgtctg caaccggcaa gaatgctacc
     2221 gagacttttg taaagagaaa tgcagggagt tgcagcatat tgcatcttgc cacacacggg
     2281 acatacaact acaatcagcc gctctattcc tgtcttttat ttcctgcaac tgatgaagat
     2341 gatggtcgtc tcaatgtctg ggaggttctt gagatgaaac tgaatgcgaa acttgttaca
     2401 ctgagtgcat gtgagactgg acttggcaac gtaacgcgtg gtgatgagat gaccggactg
     2461 agtcgtgctt ttctttttgc cggctctccg tccgtggtgg tgagcctctg ggctgttgca
     2521 gattatccaa catcatttct gatgtcggct ttttacagga atctcaagtc atatccagcc
     2581 agcgaagcct tatcacttgc ccagagagag accatgaaaa aataccccca acccctttac
     2641 tgggcacctt ttgtgcttat cggaaacggg aatgtgaaag ccgagtgagt tttatatgtt
     2701 aaatttcttg atgcattgat atttttttat ggttgtagat tccttgttga aggttaattg
     2761 atgagtaata aattgttatg atatttttta tatgtcaaat gcttcaggca ttgtcattaa
     2821 gattttattt ttaaaaaaat agtctgcaga atcttgagaa gaaactaaga caccaacatt
     2881 actcaggata ataactcacc actttaaatt tttttaggcc atccttgcat ttgtgactat
     2941 tttataactt tgaaaagttg taataaacct ttacatttta taagtttgat aaagttcaga
     3001 gatatagaaa atgaagagtt tcttgtaagg agcctttcaa agggaaacat tctggcgttt
     3061 aatacattat ttaaagagta cagcggacgg ttattccggt tcgccaatgg atatttgagg
     3121 tcagaggctg aagctgaaga aattgtacag gaagttttca caattatatg ggaaaaaaga
     3181 aaggacttaa aagatgaatt atcatttaag tcgtacctgt ttactattgc atttaacatt
     3241 attaaaaagc atttcaggag aaaggcttat ctggcagagt atttaaattc agaaatttat
     3301 tcagacctcg atattagtac tactgataag atagattatg attcggtttg cgattttatc
     3361 aggaaactag ttgacagatt gcctgacagg cgaagggaga tatttattaa aagcagattt
     3421 gagggattga gcataaaaga aatatcagaa gagcttggaa taagtcataa aacagttgaa
     3481 aatcagataa cagccgctct taaattttta aaagaaaatc ttaaacgtga atttctgcct
     3541 gcagttctat tcttttttct gtttatagct taattttttt ttattccctg ataggtgtat
     3601 tatttcattt atgaatataa ctattgtaaa aaagcaataa aaatatttgt ttggttaatt
     3661 caaggggtca gaggtcaggt tagtttggtt agggtttgaa gcagttctct catgagagaa
     3721 ctgcttagat ttgaaaaaaa attaataaat atataagtta ggatttatga aagaagagaa
     3781 ggtggatttt gataagctaa aagatttttt tgaaggtcgt aataatgatt ttgagtatat
     3841 aaagaaagta ttttgtgaca gggagaaaga agatgaactt aaaaaagttt tatggaggca
     3901 gtggtatgaa attttaaaag aggatattga aagtgaaaaa gatttgagcc atattttgta
     3961 caggattcat tatgaaatta atagtaggaa agagttcatt gatagaagag gaaagattca
     4021 taatatttta agagcattta cccggattgc tgcggtattg tttattcctt tgctgatata
     4081 ttctggttat cagtttatca gaagttcagg tactggaata gccatgccgg tcgagattac
     4141 agcaccagca tgggcacggg ttcagtttac tctgcccgat ggtaccagag gatggctgaa
     4201 caacaggtcg gttttaaaat atgaggcagg gataaatttt aacagggagg ttattttaga
     4261 tggagaagct tactttgatg ttgcaaaaga aaagaggaag cagttcagag taaaaactga
     4321 tgaagttatt attacagcac tgggtacaaa atttaatgta gcctcttaca ataatgagaa
     4381 aaatgttgaa gttgttcttg aagaaggttc tctgatatta accgataagg acaatagata
     4441 cacctgtcag atgaaaccaa gtgaacttgt tatttatgat aaaacaagcg ggcatctttc
     4501 aaaggatact gtacaaacta acaaatacac ctcatggaag gaaggaaggc tggtattcag
     4561 aaatgatcca atagatgtta ttgcccgcag gttggaaagg tggtataata ttgaggttga
     4621 gttgaaagat attttcccgg acgacataag acttcgtgcc acttttatag atgaagattt
     4681 atatgaagtt ctagaaattc tgaagcgcag tctgaacatt gactaccaca ttaatgagag
     4741 aagccttgga gatgatgggg ttttctataa gaaaaaagta atattgatga gtaaaaaaga
     4801 tatataatca cacttaaaat ctgttcccat gaaatatgat tcatcataag aggtatgaaa
     4861 aaacaggaaa gtggtgtaga cactttcctg ccattcagct aagttaatta attaaaaata
     4921 acctaactaa aacattgcaa atttatgaaa aaaaaccaat ttaacttaac attaaggaag
     4981 ttaatcatgg aacttaaaat ttcaatcctg attattcttt taagcgtatc aagtgttttt
     5041 gcgggtagat catattccca gacagcaaag atttcgcttg atataaaaga caagaccctt
     5101 gaacaggtta tggatgagat agagaggcag agtgagttct atttcatttt taaccagaag
     5161 cagattgatg tcaacagaat tgttgatata caggctaata ataagcgtat tacagatgtt
     5221 cttcctcttc tttttgaagg gacagatgtt aattatgcaa taattgatag aaaaatactg
     5281 ctaactactg atccgatttc aaaagattta ttgaaaaagg acctgaaaat taatcttaat
     5341 gaagaacagc aacagatgac cgtaacaggt gtggttactg atgcatcaac aggagaagta
     5401 ataccaggag ttaatgttgt ggtaaaaggt actacaatag gtacgataac tgatgaaaaa
     5461 gggaaatatt caataaatgt tcctgataga aatgcgattc ttgtgttttc atttatagga
     5521 tataatcctc aggaaatttt attaggaggc agaacgttac ttaatgttgc attaagttta
     5581 gacgtaaaaa gacttgagga gatagttgtt gttgggtatg gcgttcagaa gaagcttaac
     5641 cttacagcag ctgttgatca ggttacaagt gaagtaatta ctaacagacc tattccaaat
     5701 attacacagg gtctgaaggg agttatacct aatctaaaca ttaatttact agacggaaaa
     5761 ccaattcaag caccaagttt taacataaga ggtacgacat ctatcggaca gggcggtaat
     5821 gcactggttc taattgatgg tgttgaaggt gatcccagcc ttttgaatcc aaatgatatt
     5881 gccagtatat ctgttttaaa ggatgcagca gccgcatcaa tttatggagc tagaggtgca
     5941 tttggtgtag ttcttatcac aaccaaaaat cctgaaaaag gaagaacagg cgttcaatat
     6001 tcaactaact actctttaaa aaggcccatt aaactacctg attatgttac tgatggatat
     6061 acatacgtaa aaatgtttgt tgaagcattt cttaacggtg atgggtcatt ccctcagaat
     6121 attaataaaa cacagaaagt gtcccaggct tatctggatg aatttaaaag aagagtagaa
     6181 tcgggtcagc catataatac tgttgaggtt gatcctgtaa caggtgaata tgtatattat
     6241 gctagtaccg actggtacga tcttttatat aaagatttta cttcttctat tgaacacaat
     6301 ctggcaatat caggaagcag tgataaggct gcttatcttg tttcagggag aataatggga
     6361 cagaacggct tatttcgcta taatactgat gactacgcaa tgcaaaatct gagggctaaa
     6421 ggttctgtaa atatttttaa atggcttgag gtgacaaaca attcagatta ttctcatatg
     6481 tcttatcata atccaatgaa tgtgggagag ggaagcggaa tatggaggaa tattgctgat
     6541 gaaggacatc ccagcgctcc tctctttaat cctgacggaa ctttttcaat gtcagctgcg
     6601 tatactgttg gagattttgt ctacggaaaa aacggtattg acacaaaaag aactgtaatt
     6661 aaaaatacca ccggggctac agcgtctttt tttgataaca aactcagaat taaaggtgat
     6721 tttacattcc agaatacccg ttacgacagg attagaaaaa gggttcaggt gccatatagt
     6781 aataagccag gtgtgatagc ctatgtagga acaaaaacta atgacctgac ttatgaagat
     6841 agaaaaactg attacctggc tacaaatata tattctgagt atgagaacaa ttttggaaat
     6901 aatcactatc taaaattcat gattggatac aactttgaac aatccaccta caataggttg
     6961 tatgttttaa gaaatggtat aatctatccc gacgcaaaag atattaatct ttgcctcgga
     7021 caatccattg aaacaagtgg tggttatgaa aagtggatag ttcttggtgg attctcacgg
     7081 ttgaattatt cttttaagga cagatacctg atcgaaatca atgcaaggta tgatggttcc
     7141 tcaaaatttc cttcaaatca gagattcgca ttcttccctt caatatcggc aggatggaga
     7201 gtatcaaaag aatctttctt taaagtatca cccaaattag taaatgactt aaagataagg
     7261 gcatcctatg gatctcttgg taatggaaac attgcttcat atgtatatca ggaacaattt
     7321 tcaatt
//
LOCUS       DAOSNB010000107         7321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10182_length_7321_cov_5.227773, whole genome shotgun sequence.
ACCESSION   DAOSNB010000107 DAOSNB010000000
VERSION     DAOSNB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_7321_cov_5.227773"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1283)
                     /locus_tag="PLG42_05675"
     CDS             complement(21..1283)
                     /locus_tag="PLG42_05675"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOU30731.1"
                     /translation="MKMIQDFSFKGLKAIVRVDFNVPLDKKTLEVTDDTRIRGALPTI
                     KKIIGDGGACILMSHLGRPEGRQEKYSLKPVIPVLEKHLGMKVKFADDCLGEATEKMV
                     AELKPGEVLLLENVRFYPEEEGKPILPETATEEEKKAAKAELKVKQKEMAKKLASYAD
                     VYVNDAFGTAHRAHATTAVIADYFPEDKKMFGFLINSELAAMDRVLNNPQRPFTAIMG
                     GAKVSDKIMLIENLLSKVDNLIIGGGMTYTFVKAMGGKIGKSLCEEDKLDLALNLINK
                     AKEKGVNLYLPVDSVNADKFENDAQTSVSPVDDVPDGWMGLDIGEKTIEKYREVIMNS
                     KTILWNGPMGVFEMEKFSKGTVAIARAIAEATEKGAYSLVGGGDSVAAVNKYNLADKI
                     SYVSTGGGAMLEYMEGKKLPGITAIRGE"
     gene            1501..2010
                     /gene="tpx"
                     /locus_tag="PLG42_05680"
     CDS             1501..2010
                     /gene="tpx"
                     /locus_tag="PLG42_05680"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOU30732.1"
                     /translation="MATIKFKGNPVTTYGNLPVTGARAPEFVLVKTDLSNFSLADAAG
                     KKLILNIFPSLDTSVCATSVRKFNELAAGKEDTLVLAISKDLPFAHNRFCSTEGIKNV
                     IPLSGFRNSDFGKDYGVEMTSGPLAGLYSRSVVVIDRNGTVRYTEQVPEITQEPDYEA
                     ALKAVEQLP"
     gene            2040..3173
                     /gene="holB"
                     /locus_tag="PLG42_05685"
     CDS             2040..3173
                     /gene="holB"
                     /locus_tag="PLG42_05685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOU30733.1"
                     /translation="MNFARIPGHQETIEKLIKSVKENRVSHAQLFTGPEGCGSLAVAL
                     AFAQYISCENRHEKDSCGTCKSCIKYEKMIHPDLHFVYPVIKKKGSSEEPTSDDYLPQ
                     WREFISNSPFRSINEWFEMIGSEESGQGMIYTAESSEIIRKLSLKPYESDFKIMIIWL
                     PERMNQTSANKLLKLVEEPPEKTLFLMVSDEPDKIIPTILSRCQLVKIPAFKNEEIKS
                     YLRNTLGATEKEADEIARLSQGNLLRAISIFEKDEATTQNLENFRTLMRHAWKRDIAA
                     MIEWADRMASTGRETQKNFLEYSLRLLRENLMLSLKQHQKELVFLSDEEADFSEKFHP
                     FITTGNCNKLAEEFNKAALHIEANGYAKLVFLDLALKVTKLIR"
     gene            3326..4390
                     /gene="ricT"
                     /locus_tag="PLG42_05690"
                     /pseudo
     CDS             3326..4390
                     /gene="ricT"
                     /locus_tag="PLG42_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            4386..4877
                     /locus_tag="PLG42_05695"
     CDS             4386..4877
                     /locus_tag="PLG42_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOU30734.1"
                     /translation="MTGEKIKPAATALLCLVLIFTGCDRKIVYTDFVKIPEENWTIDN
                     VISFAPVITDTSSVSNVFITIRTGVEYPYQNIWLFVSTYSPSGRTITDTVEYMLADAR
                     GKRTGRGFGNIKENDLIFRKGVYFPEKGTYIINVRHGMRTGNLKGVYNVGLRIEKAEI
                     PVK"
     gene            4888..5589
                     /gene="trmB"
                     /locus_tag="PLG42_05700"
     CDS             4888..5589
                     /gene="trmB"
                     /locus_tag="PLG42_05700"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004349831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOU30735.1"
                     /translation="MGKNKLARWAEIKTFNNVIEPDFQSIRNSPHPLKGLWQKEYFKN
                     NNPIILELGCGKGEYTTGLAEIMPAINFIGIDIKGARLWRGAKTAIEKNLKNVLFIRT
                     RIEFITNLFAEDEIDEIWLPFPDPHEKKRNISKRLTSPLFLNKYRTFLKNGGLIHLKT
                     DNKLLYEYTLNLVSYNNLEIICSTPDVYTSHPDNRILTLRTFYEDGFLKEGKKIHYLS
                     FRLDKKREIIDYEQK"
     gene            5576..5956
                     /locus_tag="PLG42_05705"
     CDS             5576..5956
                     /locus_tag="PLG42_05705"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOU30736.1"
                     /translation="MSKSNRSQNISFFERVYEVVRQIPYGRVTSYGAIARYLGTGQSA
                     RMVGWALNVCNHASIPAHRVVNRNGLLTGKHHFGTPLLMQQLLENEGLIIEDDRILNF
                     HERFWDPSKELSSLNKFCHNTKND"
     gene            5994..7097
                     /locus_tag="PLG42_05710"
     CDS             5994..7097
                     /locus_tag="PLG42_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOU30737.1"
                     /translation="MFTEEQIKNALEKVIHPEKKENIISLGIVQQIESDDTGITITLL
                     REKASDPFFSSIKSNITRAIKDTLGTDAVVKEIKVTEKSKPAEEIRAVDDNLPGVKNI
                     IAVASGKGGVGKSTIALNLAIGLSLKGYKTGLLDADIFGPSVPVMLDVKDFRPQVKNI
                     NGKDIIAPLEKYGIKVLSIGFFVEPDSAVVWRGPMASNFLKQMLSMTDWGNLDYLIID
                     LPPGTSDIHLTLVQEVAVTGAIIVSTPQEVALADAVKGIAMFRNEKINVPVIGIVENM
                     SWFTPAELPSNRYYIFGKEGCRALSEKMNVPLLGQIPLVQSIREGCDTGRPVVLDSNN
                     PASEAFLALAEKVVEKVSERNKNIKPTERVKIN"
     gene            7104..>7321
                     /locus_tag="PLG42_05715"
     CDS             7104..>7321
                     /locus_tag="PLG42_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOU30738.1"
                     /translation="MSENNNIRERVIKSLERIRPYLQSDGGDISLVEITEDNTVKVKL
                     QGTCHGCPYSLYTLKAGVEQALIKDVPE"
BASE COUNT         2363 a         1521 c         1477 g         1960 t
ORIGIN      
        1 gcctcaggaa ccgcaaatat ttactcaccc cttattgctg tgataccagg tagctttttg
       61 ccttccatat attcgagcat ggcgccaccg cctgttgaaa catagctgat cttatcagca
      121 aggttatact tattaactgc agctactgaa tcgccgccac caaccagact atatgcgcct
      181 ttctctgttg cttctgctat tgctctggca atcgccactg tacccttcga gaatttctcc
      241 atttcaaaaa cacccatagg accattccat agaatagtct ttgagttcat aataacttca
      301 cggtattttt caattgtttt ttcacctatg tcgagtccca tccatccatc aggcacatca
      361 tcaaccggcg aaactgatgt ctgagcatca ttttcaaatt tatcggcatt tacactgtcg
      421 acaggcagat agagatttac acctttctct ttggccttgt tgatgaggtt gagtgccagg
      481 tcgagtttat cttcttcaca gagtgatttt ccaatttttc cgcccattgc cttcacaaag
      541 gtgtaggtca tacctcctcc aattataagg ttatcaacct tacttaaaag attctcaatc
      601 agcataattt tatccgacac cttagcccca cccatgatgg cagtaaacgg cctctgtgga
      661 ttgttcagca ctctgtccat ggcagcaagt tcgctgttga tgaggaatcc gaacatcttt
      721 ttgtcttcgg ggaagtaatc tgcaataact gccgttgtag catgtgccct gtgggctgtt
      781 ccgaatgcat cattaacata aacatcagcg tagctggcca gtttcttcgc catctctttc
      841 tgtttcacct tcagttcagc cttagctgct tttttctctt cctctgttgc tgtttccggt
      901 aaaatcggtt ttccttcctc ttcgggatag aatcttacat tctcaagcag cagtacttca
      961 cctggtttca gctcagccac cattttttct gttgcttcac ccagacaatc atctgcaaat
     1021 ttcactttca tcccgagatg tttctcaagt accggaatta ctggtttcag cgaatatttt
     1081 tcctgacggc cttcaggcct cccaagatgc gacatcagga tgcaggcccc accatcacct
     1141 ataatcttct tgatagtcgg tagagcaccc cgtatcctgg tatcatcagt cacttccagt
     1201 gtctttttgt cgagtggaac attaaaatcg acccttacga ttgctttaag acctttaaaa
     1261 ctgaaatcct gaatcatttt catataactc ttattttaat tgtttataag catcttttaa
     1321 tgcaaaccta tgcatttttt ggtaaaataa agaaaagcaa atatacaaat aaattgattc
     1381 aggctgaaac aagtaaacac cagtttgaaa acaacttcat gaactttacc tgaatcttcg
     1441 ggtttataca tttaataaaa ttcgtttaac taagtaaact ttatttaatt ttgaaagcgt
     1501 atggcaacaa ttaaattcaa aggtaacccg gttaccactt atggaaatct tccggttacg
     1561 ggagccaggg ctccggaatt tgttctcgtt aaaactgacc tgagcaattt ctctcttgca
     1621 gatgctgctg gcaaaaagct tatcctgaac atttttccaa gccttgacac ttctgtttgc
     1681 gcaacgtcgg tgagaaaatt taacgagctc gctgcaggaa aagaagacac acttgtcctt
     1741 gcaatatcta aagatctgcc ctttgcacat aacaggttct gttcaactga aggcataaaa
     1801 aatgttatac ctctgtctgg ttttcgcaac agtgatttcg gaaaggatta tggtgttgaa
     1861 atgaccagcg gccctcttgc aggattatat tcaagaagcg ttgtggtaat tgacagaaac
     1921 ggcacagtca gatacaccga gcaggtacct gaaataacac aggaacctga ctacgaagcc
     1981 gccctgaaag ctgttgaaca gttaccttaa aaattgaatc caataaccta cataccgcca
     2041 tgaattttgc acgaatacca ggccatcagg aaactattga aaagcttata aagtcggtta
     2101 aggaaaacag ggtgagtcat gcccagcttt ttaccggacc tgaaggatgc ggtagtctgg
     2161 cagttgcact tgcttttgca cagtacataa gctgtgaaaa caggcacgaa aaagattcat
     2221 gcggtacatg taaatcgtgt atcaaatacg agaagatgat tcatcctgac cttcattttg
     2281 tataccctgt tataaagaag aaaggaagca gtgaagaacc tacaagcgat gactatctgc
     2341 cgcagtggag agaatttatt tcaaattcac ctttcagaag cattaacgaa tggtttgaaa
     2401 tgataggttc agaagagtca ggtcagggca tgatatatac tgccgagtca tcggagatta
     2461 tcagaaagct tagcctgaag ccatatgaat cagatttcaa aattatgata atctggttgc
     2521 ctgaaaggat gaaccagact tcagcaaaca agctgcttaa actggttgaa gagcctcctg
     2581 aaaagactct gtttcttatg gtaagtgacg aacccgacaa aataatacct acgatattat
     2641 caagatgcca gctggtgaaa attcctgcct ttaaaaatga agagataaaa agctatctca
     2701 gaaatactct tggggcaact gaaaaagaag cagatgaaat tgcacggttg tcgcagggga
     2761 accttctcag ggctataagc attttcgaaa aagatgaggc aaccactcag aacctggaaa
     2821 atttccgcac tcttatgcga cacgcatgga aaagagatat tgcagctatg atcgaatggg
     2881 ccgatagaat ggcttcaaca ggcagggaaa cccagaaaaa cttccttgaa tactcattga
     2941 gactgctcag ggaaaacctg atgctgtcac tcaaacagca ccagaaagaa ctagtcttcc
     3001 tctcggatga ggaagctgat ttctccgaaa aatttcaccc ttttataact actggtaatt
     3061 gcaacaaact tgctgaagaa ttcaataaag ccgctcttca catcgaagca aacggttatg
     3121 caaaactggt attcctcgac cttgccctca aggttacaaa actcattcgc tgaactcttt
     3181 gcgttgaaat tgccatgtcg taacaattaa ttacctttgc agttcagtat taagcagaga
     3241 agagcaaatg gaagataaca ggcaacaaaa taaacctgaa accgaaccaa aggtagcaga
     3301 accagcatac tgtcccggtc tgaataaact tgattcttat aactggctga aaaaccttcc
     3361 tcctgaagta actgacaatg aaattgttga agtaagattt aaaaacacaa ggaagggttt
     3421 ctaccgaaat gtaaacaatt tacggctgga aataggcgat atagtggcag ttgaagcttc
     3481 tccaggacat gatattggca gggtatcact aaccggtccg ctggtactga agcagatgaa
     3541 aaagcttaaa gtcattcctc cactggagga aataaaaaaa atataccgta aagctaaacc
     3601 tgttgatctt gagaaatggg aagaggcaaa gaagctggaa atacccacaa tgctgaaatc
     3661 cagaaaaata gcagaaagcc tcaatctgaa tatgaaaatc agcgatgtgg agtaccaggg
     3721 cgataaaact aaagctattt tctactacat tgctgatgaa agggttgatt tccgccagct
     3781 tatcaaaatt ctagctgagg aattcaaggt aaggatcgag atgcggcaga taggagcaag
     3841 gcaggaggca ggacgtatag gaggaatagg ctcatgcgga agggaactct gttgttcgac
     3901 ttttattaca aatttttcat cggttaccac aacacatgca agataccagg aactcacact
     3961 taatccccag aaacttgcag gccagtgtgg caagcttaag tgctgcctga gctacgagtt
     4021 cgactgttat gtggacgccc agaaaaactt ccctcccaga gatgttcctc ttgaactcac
     4081 cgaccgaacc ctttatttcc agaaaatgga agtccacaaa gcaatctact ggtactcaaa
     4141 agaaccaaat acagctgcaa atctgatgtc catatctgtt gacaaagtaa gagagataca
     4201 ggaactgaac caaaagggta tcaagccaga ttttctcttt gcttctgaag aatcatggaa
     4261 tgaagaacct ttagcgggtg acttgcttga agataatagc atcactcgct tcgacaggca
     4321 acagatggct aacgaagaac tgaaacaaca gaaggattct caaaacagga aacgtaaaaa
     4381 acatcatgac aggagaaaaa attaagcctg ctgcaactgc tctgctctgt ctggtgctta
     4441 tttttaccgg gtgtgacaga aaaattgttt acaccgattt cgttaaaatt ccggaagaaa
     4501 actggacaat tgacaacgta atatcatttg ctccggttat tactgatacc tcatcggtaa
     4561 gcaatgtttt tataacaatt cgcacaggtg tggagtatcc ttatcagaat atctggctgt
     4621 ttgtatcaac ctattctcca tcgggcagaa caataacaga cactgtcgaa tacatgcttg
     4681 ccgacgccag aggaaagcga accggcagag gcttcggaaa catcaaagaa aatgatctta
     4741 tcttcagaaa aggggtctat tttcctgaaa aaggaaccta tataataaat gtccgtcatg
     4801 gaatgagaac tggaaacctt aaaggggttt ataacgtggg gcttcgtatc gaaaaagccg
     4861 aaatacctgt aaaataaact gcttgctgtg ggaaaaaaca aactcgcccg ctgggccgaa
     4921 ataaaaactt ttaataacgt tatagaacct gattttcaaa gtattagaaa tagtccccat
     4981 ccattgaagg gactctggca aaaagaatat tttaaaaata acaaccctat tatcctcgaa
     5041 ctgggatgcg gcaaggggga atatacaacc ggtcttgctg aaataatgcc cgcaattaat
     5101 ttcatcggga tcgatattaa aggagcaaga ttatggagag gagctaaaac tgccattgaa
     5161 aaaaacctga aaaatgtact ttttatccgt acacgcattg aattcattac caatctgttt
     5221 gcagaagatg agatcgatga gatatggctt cccttccccg acccccacga aaagaaacga
     5281 aacatttcca aacgtctgac cagcccctta ttcctaaata aataccgtac cttcctgaaa
     5341 aacggcggac ttatccatct taaaaccgat aacaaactgc tatatgaata tactcttaac
     5401 ctggtttcat ataacaacct tgaaataata tgctctacac ctgatgtcta tacttcgcat
     5461 ccagataaca gaatattgac actcagaact ttctatgaag atggattttt aaaggaaggc
     5521 aagaaaatcc attatctttc attccggctc gataaaaaaa gggaaattat tgattatgag
     5581 caaaagtaac cgttctcaaa acataagttt ttttgaaagg gtttatgaag tcgtgcggca
     5641 aattccatac ggaagggtta cttcttatgg cgcaattgca cgatatcttg gcacaggaca
     5701 gtcggccagg atggtgggat gggctctcaa tgtatgcaat catgcttcta taccagccca
     5761 ccgtgtagtc aaccgcaacg gactgctgac tggaaaacat cattttggaa cacctttact
     5821 gatgcaacaa ttacttgaaa atgaagggct tatcattgag gacgatcgta ttcttaactt
     5881 ccatgaaaga ttctgggacc catcaaagga attaagcagt ctgaataaat tttgccataa
     5941 cactaaaaat gactaatatt gtagcttatt taaaattaat ttaaataaaa ataatgttta
     6001 cggaagaaca gataaagaat gcgcttgaga aagtaatcca tcccgaaaaa aaagaaaata
     6061 ttatttctct gggcattgtg cagcaaattg aatcagacga tactggtatt acaattactt
     6121 tgctgagaga gaaagcttca gatcctttct tctcgtcaat aaaaagcaat atcactagag
     6181 caattaagga tacacttggc actgatgctg tagtaaaaga aatcaaagta actgagaaaa
     6241 gtaagcctgc agaagagatc agggctgttg acgacaactt gccaggtgta aagaatatca
     6301 ttgcagttgc ttcaggaaaa gggggtgtgg gcaaatcaac aatagcactt aatcttgcaa
     6361 taggactttc attaaaaggt tataaaacag ggttgctcga cgctgatatt ttcggccctt
     6421 ccgttcctgt tatgcttgat gtaaaagatt tcaggcctca ggtaaaaaat attaatggca
     6481 aggatattat tgcccctctc gaaaaatacg gcattaaggt actttccatt ggatttttcg
     6541 ttgagcccga cagtgccgtt gtgtggagag gtccgatggc atcaaatttc ctgaagcaga
     6601 tgttgtcgat gaccgactgg ggcaaccttg attatctcat tattgatctt ccaccgggca
     6661 caagtgatat tcatctcaca cttgtccagg aagttgcagt aaccggtgcc ataattgtaa
     6721 gtactccgca ggaagtagca cttgccgatg cagtaaaggg aatagcaatg ttcagaaatg
     6781 aaaagataaa tgtccctgta atcggaatag ttgaaaatat gtcgtggttt acaccagccg
     6841 aactaccttc aaacaggtat tacattttcg gaaaggaagg ctgcagggcg ttgtcagaaa
     6901 aaatgaatgt gccccttctt gggcaaatac ctcttgtgca gagtataagg gaaggatgtg
     6961 acacaggacg accggtcgta ctcgacagta acaatcctgc ttcggaagca ttccttgcac
     7021 ttgcagaaaa agtggttgaa aaagtttcag aacgcaataa aaacattaaa ccaacagaac
     7081 gagtaaaaat aaattgaaaa aagatgtctg aaaacaacaa cattagagaa cgagttatta
     7141 agtctctgga aagaataagg ccctatcttc agtctgacgg aggggacata agtcttgttg
     7201 aaattacaga agataacact gtaaaagtga agctgcaggg cacctgccat ggatgcccgt
     7261 acagcctcta tacattgaaa gctggcgtgg aacaggctct tataaaagat gttcccgaga
     7321 t
//
LOCUS       DAOSNB010000108         7298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10218_length_7298_cov_3.569515, whole genome shotgun sequence.
ACCESSION   DAOSNB010000108 DAOSNB010000000
VERSION     DAOSNB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10218_length_7298_cov_3.569515"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLG42_05720"
     CDS             complement(<1..223)
                     /locus_tag="PLG42_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30739.1"
                     /translation="MKKLIILSGFILLIISGCKSRQMQNTEYGSFLYDHKAMPMDTSN
                     SLQYKWNHKEILESVLIDGMESLENWELTF"
     gene            388..630
                     /locus_tag="PLG42_05725"
     CDS             388..630
                     /locus_tag="PLG42_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30740.1"
                     /translation="MALLKYIERVRRIDMLIHMKATGPPVEFASKLGIRRSTLFQSLQ
                     EMKEMGVDIRYSNTRETYYYADGRRIEIKVEKTTGS"
     gene            731..1393
                     /locus_tag="PLG42_05730"
     CDS             731..1393
                     /locus_tag="PLG42_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30741.1"
                     /translation="MKVLEGYFGLERNKLLFIMISVNLLLIWLSKSVLLNETVFYNTY
                     SEQLTYERSMELFKRIMDLSWVSYLFSPLVLIIKFSVLSLIIYTGFFLSDLHGKVSLG
                     MIFTTVVASEIIIVFASLAKFLWFTFFAGNYTLDDMNFFYPLSLINLFKKGEVAQYWI
                     YPLQSVNLFQVAYILMLATGLRTISSVKREKTDTIVLLTYGTALILWIAVIMFITIDI
                     QS"
     gene            1390..1923
                     /locus_tag="PLG42_05735"
     CDS             1390..1923
                     /locus_tag="PLG42_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOU30742.1"
                     /translation="MKKILTFLITVSVCAILFILAKGIFLKVLKANDRDDRIKTLPSF
                     SLGTLNGEIFNSADLKNGPLLLVYFHPDCDYCQYEISSLIRHDIQSKGVKVLLVSNAL
                     PETIDEFMEQFETGQDEFCILSDSSLVFANLFGISVIPVSLIYNENLELIKFFKGEVS
                     PNAILNFLNDVCQYQEN"
     gene            1895..4069
                     /locus_tag="PLG42_05740"
     CDS             1895..4069
                     /locus_tag="PLG42_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU30743.1"
                     /translation="MMSVNIRKIKEKALLQHDSTDCGAACLASAIRYFGGESGIERIR
                     KLSGTSQSGTTMLGLYQAAVASGLKAQGYEASVESIKSFKGLLILHVSPEIRYDHYIL
                     SFGYEDGRFVLWDPANGLTTKTAEELEKIWPGHKCLSVEPGEGFVFKKEEDKGKRKWL
                     INTIKPDRELLMISILLGLLISALGAVMAIFTQKLIDRILPSGEVKVLLISSVLVFVL
                     LISRVVLLSLRQYILLSQGKSFNIRIVDGFYRSLLGLPKLFFDTRKTGDLVARLNDTM
                     RIQRVISDLISIYFIDIVVLIVTSVLIFIYSLPAGIISVIAIPLIYLLVYRWNKRIIS
                     SQNELMTGYAMNESNFINSLRGIAEIKCMNWQGYYSLRNESVFSNFQEKFFSLGKIRV
                     SLGMLTNLAGTLYLIVLLIYSSLKVMDLQMTQGELMAILSLSSTLIPSVMNLALISIP
                     LSEAKVAIARMFEFTRISPEETDSGNIKENIAIEKISLNNMKFRFPGQKLFLTDINLE
                     IEKGQIIALVGESGSGKSTLVNILLRFLIPESGDIIINDIVPVNQIMPGVWRRSVGVV
                     PQDIHIFNGTILENLIPEPSEKEVEILLRLISDYGLESFIEALPLGLVTLVGEDGVKL
                     SGGQKQVIAFLRALINEPDILLIDEGTSGMDKDTEDLMLKMLISLKAKLGILLVTHRI
                     SLIKKICDRIYILEDRTIKESGTHYELISSENIYKRYWEELN"
     gene            complement(5233..6780)
                     /gene="ltrA"
                     /locus_tag="PLG42_05745"
     CDS             complement(5233..6780)
                     /gene="ltrA"
                     /locus_tag="PLG42_05745"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOU30744.1"
                     /translation="MKKQQRSEAQAECASSAECRTSWKGINFARCERKVRKLQVRIAK
                     AQKERRYNKVKALQHLLVTSFEAKALAVRKVTTNKGKRTAGVDHVLWNTNAKKINAVC
                     SLKRRGYRALPLKRVNIPKKNGKMRPLGIPTMKDRAMQALYLMALEPVTETTADANSY
                     GFRKFRSTADAIDALHRWLSRDCSPQWILEGDIKGCFDHISHEWLLDNVRIDKYILEK
                     WLKNGVVFNKLLQPTVKGTPQGGIISPTLANATLDGMERMMKERYKVSYIDGRLYYPK
                     VNCVRYADDFNVTADKRETLEEIKRMLIEFLGKRGLTLSEEKTLITHISEGFDFLGFN
                     VRKYNGTLLIKPSPKSLKRFTEKLHEVVFGKNKAVAQQKLIEALNPVLRGWGNYYSGV
                     VSKTTFSKIDHILTNQLKRWSYRRHTNKSRRWIKDKYFIKVGNRDWIFGFKHKDCEHD
                     AIFTLMKLADIPIKRHIKVRCEANPYDPTWDAYFWKRKQKCNRTRTSRKGTLSEIVSC
                     KGADEPI"
BASE COUNT         1976 a         1507 c         1528 g         2287 t
ORIGIN      
        1 cgaaagtgag ctcccagttt tccagcgatt ccattccatc aataagaacg gattccagga
       61 tttccttatg attccattta tactgcaggc tgttggaagt atccattggc atggctttat
      121 ggtcatacag aaaggaacca tattccgtgt tttgcatctg tctgctctta cagccggaaa
      181 ttatcagcag gatgaagcct gaaaggatta ttaatttttt catggtttat taaaatcacc
      241 attcaattca caacatatca aaattactgc taatctttca catacaggtg aaataaataa
      301 tcataaaaat tttattgagt ttctctggac tcatataata tatttatcta tatttgatca
      361 gctgcgtttc tttaaaacca aaatatcatg gctctcttaa agtatatcga aagggtgcgc
      421 cgtattgata tgcttataca tatgaaggca acaggcccgc cggtggaatt tgccagcaag
      481 ctcggcataa ggcggagtac cctgtttcaa tcccttcagg agatgaagga aatgggagtt
      541 gacatcagat attcgaatac aagggagaca tattattatg cggatggaag gagaattgaa
      601 ataaaagttg aaaagacaac cggcagctga tcgtagtgtt tggtttttct gactcttagg
      661 gtattttgac aaatgctcta tgattgtacg acaacaagtt acaataaata atatttcaat
      721 tcgctgaaca atgaaagtgc tggaaggata cttcggcttg gaaagaaaca agctgttatt
      781 tataatgata tcagttaatc tgttgctgat atggttgtcg aaaagcgtac tcttaaacga
      841 aactgtattt tacaatacct acagtgaaca gctgacctat gaaaggtcca tggaactttt
      901 taaaaggatc atggacttat cctgggtcag ctatctgttt tccccgcttg tcctgatcat
      961 caaattttcg gttctgagcc tgataattta tacaggattc ttcctgagtg acctgcacgg
     1021 aaaagtgtca ctgggcatga tctttaccac agttgttgct tcggaaatta tcatcgtgtt
     1081 tgcttccctt gccaaatttc tgtggtttac attttttgcc ggtaattaca cacttgacga
     1141 tatgaatttc ttctatcccc tttctcttat taatctgttt aagaaaggtg aagtggctca
     1201 gtactggatc tacccccttc agtcggttaa tctgttccag gtggcttaca tactgatgct
     1261 ggccacaggc ctgcggacaa tcagttctgt taaaagggaa aagaccgata ctatagtgct
     1321 tctgacctat ggcacggccc tgattttgtg gattgcagtg ataatgttca ttaccattga
     1381 tattcagtca tgaaaaagat attgaccttt ttgataacag tatctgtctg tgctattctt
     1441 ttcatcctgg caaaggggat tttcctgaaa gtgctaaaag caaatgaccg tgacgacagg
     1501 attaaaacgc tgccttcctt ttcactggga acgctgaatg gcgagatttt taattcggca
     1561 gatcttaaaa atggtccttt gcttcttgtt tatttccatc ctgactgtga ttattgtcaa
     1621 tatgagatat cgtctcttat ccggcacgat attcagtcaa agggtgttaa agttctgctt
     1681 gtcagcaatg ccctgccgga aactattgat gaattcatgg aacaatttga aacaggacag
     1741 gatgaattct gtattttatc agacagctcc cttgtttttg caaacttgtt tggtatcagt
     1801 gttatccctg tcagtctgat atataatgaa aatctggagc tgattaaatt cttcaaggga
     1861 gaagttagcc ccaatgcaat tttaaatttt ttgaatgatg tctgtcaata tcaggaaaat
     1921 taaagaaaag gctctgttgc agcatgattc cactgactgc ggagctgcct gcctggcttc
     1981 ggccataaga tatttcggcg gggaatcagg cattgaaaga ataaggaaac ttagcggaac
     2041 atcacaatcg ggaaccacaa tgctcggact ttaccaggca gctgttgcaa gcggattaaa
     2101 ggcacaggga tatgaggctt cagttgagag catcaagtca tttaagggat tattgattct
     2161 tcatgtgagt ccggaaataa gatatgatca ttatatatta agttttggat atgaagatgg
     2221 ccgttttgtg ttgtgggatc ctgcgaacgg actgactaca aaaacagctg aagaactgga
     2281 aaagatatgg cccggacaca aatgtctttc agttgagccc ggagaaggtt ttgtatttaa
     2341 aaaggaagaa gataaaggga agcgcaaatg gctgatcaac accataaaac ccgacaggga
     2401 gcttttaatg atcagtattt tactgggctt gctcatatcg gcactgggcg ctgtaatggc
     2461 cattttcaca cagaagctga ttgaccgtat tttgccttcg ggtgaagtga aggtcctcct
     2521 gatttcctct gttcttgtgt ttgtactgct tatttccagg gttgttttgt tgtcactaag
     2581 gcagtatatt cttctgtctc agggaaaatc cttcaacata cgcatagtgg atggcttcta
     2641 ccgatcgttg ctgggacttc cgaaattgtt ctttgataca cgtaagacag gcgacctggt
     2701 tgcacgcctt aatgatacaa tgcgcattca gagagtgata tccgatctca tcagtatcta
     2761 ttttattgac attgttgttc tgattgtaac ttctgtgctg attttcatat attccctgcc
     2821 agcaggtatc atttcagtga tcgcaattcc attgatttat ttactggttt accgttggaa
     2881 taaaagaatc atttcatccc agaatgaatt aatgaccgga tatgcaatga atgaaagcaa
     2941 tttcattaat tcccttagag gcatagccga gataaaatgc atgaactggc agggttatta
     3001 ttctttgagg aatgaatctg tcttcagcaa tttccaggaa aaattcttct ccctgggcaa
     3061 aatcagggtc agtctgggaa tgctgaccaa tctcgcggga actctttacc tgattgtatt
     3121 gctcatttat tcatccttaa aggttatgga cctgcagatg acacaggggg aactgatggc
     3181 aatcctgtcg cttagctcta ccctgatacc atcggtgatg aatctggcat tgatttccat
     3241 tccactgagc gaagcaaagg ttgctatcgc aaggatgttc gaattcacca ggatcagtcc
     3301 ggaagagact gattccggta atataaaaga gaatattgca attgaaaaaa tcagtcttaa
     3361 taatatgaag tttcgcttcc cgggccagaa gctgtttctg acagatatca acctggaaat
     3421 tgaaaagggg caaataatcg cattggtggg agaatcaggt tcggggaaaa gtactttggt
     3481 aaatattctt ttaagattcc tgattccgga atcaggtgat attatcataa atgatatcgt
     3541 tccggtcaat cagataatgc ctggcgtatg gcgcagatca gtcggagttg ttccgcagga
     3601 tatacatatt tttaacggaa caatactgga aaatctgatt cctgaaccgt cagaaaagga
     3661 ggttgaaatt ttattgcgcc ttatttccga ttacggcctg gaatcgttta ttgaggcctt
     3721 gcctctgggc cttgtcaccc tggttgggga agatggagta aagctttccg gtggtcagaa
     3781 acaggttatt gcttttctca gggcattgat caatgaaccg gatattttac tgattgatga
     3841 aggtacctcc ggcatggata aggatacgga ggacctgatg ctaaaaatgc tgatcagcct
     3901 gaaagcgaag ctgggaatct tgctggtaac tcacagaatc agtctgatta aaaaaatctg
     3961 tgacaggatt tacatacttg aggaccgtac cataaaagaa tcaggaactc attatgagct
     4021 tatcagcagc gaaaatatat acaagcgtta ctgggaagaa cttaattgaa aattctatga
     4081 atattgtatt ttttgactga tatttactca tatgctacat tttcagaatt gatttccggg
     4141 atctagaacc ctgttttgag tgcaacgtta ttttgaatga ctgtttttct ttctgcctgg
     4201 gaaggcagaa atacaggtaa acgatatttg tcatacaaac taaaagataa tgagaatccg
     4261 gatattctgg atgcgagaac cctgataacc tgagattatg aaaagatatt tctataccat
     4321 taatttcttc ttgacggcta tctttttttt tgtagcctta gtgctggatc tgaaaccttc
     4381 aatacaaggt tttagttttg ttatattttg ttcctcaatc ggggtattga taggtgatta
     4441 tttgagaatc aggaaaaata tcatattgcc agttgctgga aaaaaactca tttcctggat
     4501 tctttttata agcattgtat ttatcttatt aatactcttc ctgattgata atatattaac
     4561 aaaacagatt ctcatttttt gtactttact gacaggtgca ttaataacta tattcattgt
     4621 attttttaaa ggaagacctt tgaaaaaaga tgagcagggc aaagaccgga tccattcaag
     4681 ctcacgttag gaacaggatt atgcggcccg gattgattaa caactttttc tgtcagatta
     4741 tcctgctagt gtgacctttt tcaaattctt ttcctactgc tgtattgata tttgtattgt
     4801 tcagttataa gcaaggttaa tgaaaataac ggttgtaaat gagctaacca cccaaagtcg
     4861 agcatgtgaa agagaaaatc gacattaaat aatagagtat ccgattttta gaatcgatta
     4921 ttgtttaatc aagaagaagt tttaaatctt gaaaaacttc ctttttaaaa acaaattgat
     4981 ataaaaagct tttttgtaag ttttcccgga atagttgata ttgcatcgtt aattgtggtc
     5041 tcttgtaaag aagataatct tgctaaagac ttaagtatga attttagcaa acatcgataa
     5101 tgcacaacca agctatggat ttcgactagt tatgtaggtc agcggcgttg ccgccgcttt
     5161 ccccattaag aactgtacat gcgactttca ccgcatacag ctccgataat tcaaaattta
     5221 gttctcacaa aactaaatcg gctcatccgc tcctttgcag gaaacgattt ccgataaagt
     5281 gccctttcgg gatgtacgtg ttcggttgca tttctgcttc cgcttccaga agtaggcatc
     5341 ccaagtcggg tcgtatggat tggcttcaca cctcactttt atatgccgct taatcggtat
     5401 gtccgccagc ttcataagtg taaagatagc atcatgctcg caatctttgt gcttgaagcc
     5461 aaaaatccaa tcccgattac cgaccttgat aaagtatttg tcctttatcc atcttctcga
     5521 tttgttcgta tgcctccgat aagaccaacg ctttaactga ttggttaata tatggtctat
     5581 cttgctaaac gtagttttag acaccacacc gctgtagtaa ttgccccagc cccgcaatac
     5641 ggggttaagg gcttctatca gcttttgctg cgctaccgct ttgttctttc cgaacacgac
     5701 ttcatgcagc ttttcagtga accgtttcag gcttttaggg gatggtttga tgagcaatgt
     5761 accgttgtac ttccgaacat tgaaccccaa aaaatcgaag ccctcactaa tgtgcgttat
     5821 cagtgtcttt tcttcggata gcgtgagacc acgcttccca agaaactcaa tcaacatgcg
     5881 ctttatttct tccagtgttt cccgtttgtc cgccgtgacg ttgaagtcat cggcataacg
     5941 tacgcaattc actttcggat agtaaagcct accgtcgata tagctcacct tgtacctctc
     6001 tttcatcatc ctttccatgc catcgagcgt agcgttggcg agtgtcggtg agatgatgcc
     6061 gccctgcgga gtacccttca ccgtaggctg taacagcttg ttgaacacca ctccattctt
     6121 cagccatttc tctaagatgt acttgtcgat tcgtacgtta tcgagaagcc attcgtggct
     6181 gatgtggtcg aagcatccct tgatatcgcc ctccaatatc cattggggag aacaatccct
     6241 gcttaaccat ctgtgtaggg cgtctatagc gtcagccgta cttctgaact ttctaaatcc
     6301 ataagagttg gcgtctgccg ttgtctccgt aacgggttcc aaagccatca gataaagagc
     6361 ctgcatggct ctgtctttca tggtcggaat cccgagcggt ctcatcttcc cgttcttctt
     6421 cggtatgttg acccttttca gtgggagggc tctgtaccct ctccgtttca aggaacacac
     6481 tgcgtttatc ttcttggcgt tggtattcca caatacatgg tcaacccctg ccgtacgttt
     6541 tcctttattg gttgtcactt tccttaccgc caatgccttg gcttcaaagg aagtgacaag
     6601 aagatgctgc aaggctttca ccttattgta tcttctttct ttctgagcct ttgctattcg
     6661 gacttgtagc ttccttacct tgcgctcgca tcgtgcgaag ttgattccct tccatgatgt
     6721 cctgcattcg gctgaagatg cacactcggc ttgcgcctcg ctcctttgct gtttcttcat
     6781 actttaagtt ctaccattcg tttcgcaaag aattaccctg tggaagtctg cccgctttcg
     6841 cgcggggtga cgttcaaacc cctatccata atattacatc atggcattcg ctttctccac
     6901 aatcctttac ccgcactcca ttcggccgct cttacgattg gcttacccat ccacagttgg
     6961 ctgatgggag aagtacgggc ttaccaagtt ccgcacaagt aactgacgac cgacttagag
     7021 tccgcccttc caccggcagt gcaacatccg tgtagcacga aaataaagcg cactatcctg
     7081 actgcttgcc ttttggcatg gctcgtaaac atccgtgagc catttctttg tcacgatggt
     7141 gaagcggttc acctgcgtta ctcatatcgg tcagccttgc cattcttcac ctatgatgtt
     7201 taggctggaa cgtcgttgtc aagagggctc cgcacccgac cgttgccagt cacgcacgcc
     7261 tctttaggct actattgacg gaacaatagg ttcagacg
//
LOCUS       DAOSNB010000109         7295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10230_length_7295_cov_4.824724, whole genome shotgun sequence.
ACCESSION   DAOSNB010000109 DAOSNB010000000
VERSION     DAOSNB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10230_length_7295_cov_4.824724"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="rsmA"
                     /locus_tag="PLG42_05750"
     CDS             complement(<1..591)
                     /gene="rsmA"
                     /locus_tag="PLG42_05750"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOU30745.1"
                     /translation="MRGVRPKKRFSQHFLKDEGVARRVSESLLGEGCDSVLEIGPGTG
                     MLTGFLLKRGFSDFRVVEIDRDSVEYLKKNLPNLGNNIIEGDFLKLDLDIFPGTIAIV
                     GNFPYGISAPILFRVLKYRHKVKELTGMFQHEVAQRICAGPGSRTYGILSVLIQAYYA
                     AEYLFTVSEYAFSPPPKVKSGVIRLRRNNINKLDCDE"
     gene            complement(621..3749)
                     /locus_tag="PLG42_05755"
     CDS             complement(621..3749)
                     /locus_tag="PLG42_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU30746.1"
                     /translation="MRESVLLALIHIFAIISTVNPGGITSRGKKILRGYLRRYLNREL
                     EEEYFSLFENNLEFYSNELKNINRSDLAEEDSLITFQITNICRQIKKSLFLEERMIVF
                     LQLLEFTFEDGQISPQENTIIDIVARTFNISRKEYDNAYAFMIGRQYDKVSPDCMLVI
                     ESDNPHYRSQQEYASYPLWKHMYIRGFRGSLFVLHIESTGSLIFTYDGPLPLYFKGRD
                     IIACRPYLLERGVNIKGPGIEPIYYSKVYKAFLIDEFRERVVFEGCNIEYRFKNSENG
                     IKPMNFRLESGNLVGIMGSSGVGKTTLLNILHGKIKPEKGHIYINGYDIYKDSEHLKG
                     LIGYVPQDDLLIEELTVYQNLYFNARLCFGDYTEERLHEIVVKILTDLDLIEIKDLQV
                     GDLLNKKVSGGQRKRLNIALELMREPAVLFVDEPTSGLSSQDSHNVMMLLRKQALSGK
                     LVIVNIHQPSSDILKMFDRLWILDRGGYMIYDGDPVEALVYFKTETSQANAAESECPS
                     CGNIDTDNILEIIEVKTIDDSGYPGKERQVTPVEWYEKYKRKMMPSCSSPPQKLTLPK
                     SNFKVPGRAYQIRTFIKRNLLRKLADKQYMILNLAEAPLLAFILGYFSKYTLDSGYTF
                     ADNRNYPVFLFMAVVVALFLGLMVSAEEIFKDRKILVREKFLNISRLSYLISKVGFLF
                     VLSALQSLLFLAVASLVLEIRGMFWQQWLILFSTACYGNLLGLNISSGMRTVVSIYIL
                     IPLILVPQLLLGGAMIRFDDIHKSLTNREYVPMIGDLMGTRWAYEAIMVEGYKGNRYI
                     KPTFKYNLEIEQNNYYKDELIRNLKNEIINYILQRKNKNAGDKEVIEKKLNILKYHVN
                     QLSDITGIKPGEWVNSLNYSDFNERVADSLEVFFGEVQKYFINENKYYFRLKYETEKK
                     IAESLGNEDASKLAEKYNNSRLEDIVLNQTGLLEERPYIILKNRFVQKSTPIYMKPTS
                     KWGRSHFFAPYKQLGNLKIDTLFFNIAFIWLMTGLFFLTLYYNLLYRFIEFLERQRFP
                     FWRKFGRQLLGW"
     gene            complement(3868..4692)
                     /locus_tag="PLG42_05760"
     CDS             complement(3868..4692)
                     /locus_tag="PLG42_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30747.1"
                     /translation="MKKVFQFSITVILVIALVNMSSCKSKTGAEKQVKEQKEKEQVET
                     IKQEIEKNVYPLPTSAEVIKMLTELEVGYIIGISNPPSNAKKYIQSSAKAINIGVFGA
                     DLSYATLYNMQQDVNDYLDAIRVLANSLNMSRIYDTKLYDSIKVNFDNRDKLVKILTD
                     AFNETYQYLSENEQQNLALLVVGGAWVEGMYLTTHVSEAAYNVAGISRVLLEQKKSFE
                     LYLDITKPYLDDPMIGDFVKLLEPIKKVYEGLSTSLTEKDIKDITEAITVIREKII"
     gene            5025..6113
                     /gene="carA"
                     /locus_tag="PLG42_05765"
     CDS             5025..6113
                     /gene="carA"
                     /locus_tag="PLG42_05765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOU30748.1"
                     /translation="MQREKVKKAKLILEDGTVFEGNSFGADVPASGEVVFNTAMTGYP
                     ESLTDPSYRGQILCLTYPLVGNYGVPPAEQENGLLKYFESSSVHISGLIVSHYSFDYS
                     HWNARESLGDWLADYDIPGIYGIDTRALTKRLREKGTMLGKIEIEDKCTDFFDPNQIN
                     LVKEVSTKEKKIYGNGRWKILLIDCGVKYNIIRSLLRYDTTVIRVPWDYDYHNEEYDG
                     LFISNGPGNPKMCTVTIENIKKSLKEDKPIFGICLGNQLMALASGADTYKLKYGHRSH
                     NQPVIMSGTNRAYITSQNHGYAVDNNTLPSGWEPFFINLNDMTNEGMKHKSGLYFSTQ
                     FHPEASGGPTDTAFLFDMFISNIIKYRS"
     gene            complement(6140..>7295)
                     /gene="argH"
                     /locus_tag="PLG42_05770"
     CDS             complement(6140..>7295)
                     /gene="argH"
                     /locus_tag="PLG42_05770"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOU30749.1"
                     /translation="LHALFQKYLNRELKIGEGVEDIHSQIENELSDMLGPVGRKVHTG
                     RSRNDQVLLDIRLYTRAEIDRIAELTGYLFEKLISLSNQYREVLLPGYTHMQPAMVSS
                     FGLWFGGYAEALTDDLLLLSAARSMNNRNPLGTAAGYGTTLPLNRSLTTSLLEFEEMI
                     YNSSYASINRGKVEMAVASALASIANTLSKFCMDICLYMTAEFKFIEFPDEFITGSSI
                     MPQKKNPDVFELIRARSNFIQSLPGQISIITNNLPSGYNRDLQILKSLMINAFNDIGE
                     CIEMLIYMLGAIKIRRNILDNPQYNSIFSTEEVNRMVRNGIPFRDAYKAVSGILKDGA
                     FLRTMPSDYSHEGSIGNLCNDEIKARAEKLLAEFKTIPITALEEKLLKYA"
BASE COUNT         2108 a         1618 c         1342 g         2227 t
ORIGIN      
        1 ttcgtcacaa tcaagcttat taatgttgtt tcttctcaga cgtatcactc ctgattttac
       61 tttcggcggc ggagaaaatg catattccga aaccgtgaac agatactctg cagcataata
      121 ggcttgtata agtacactca atattccata ggttctggaa cccggtccgg cacatatcct
      181 ctgagctact tcatgctgaa acatcccggt caactccttc actttgtgac gatattttaa
      241 taccctgaaa agtatcggcg cagaaatacc atatggaaaa tttcccacta tcgcaatagt
      301 tccgggaaaa atatcaaggt caagcttcag gaaatcacct tcaatgatat tgttaccaag
      361 attgggcaag ttctttttaa gatattcaac agagtctctg tctatttcaa caaccctgaa
      421 atcgctgaag ccccttttaa gaagaaaccc ggtaagcatt cctgttcccg gaccaatctc
      481 cagtactgaa tcacaacctt ctcccagcag agattccgat accctgcggg ctactccctc
      541 gtctttcaga aaatgctgac tgaatctttt ttttggtctt acccccctca tcaggataaa
      601 aatcaacggt gataaacctc ctaccagcca agaagttgcc ttccgaactt tctccagaac
      661 ggaaatctct gtctttcaag aaattcaata aatctgtaca gcagattgta atagagagtc
      721 agaaagaaga gaccagtcat taaccagata aatgctatat taaaaaatag tgtatcaatt
      781 tttaaatttc caagctgttt gtatggagca aaaaagtggg acctgcccca ttttgaagtt
      841 ggcttcatgt aaatgggagt ggacttctgt acaaatcggt tcttcaaaat gatatatggt
      901 cgctcttcaa gtaaccctgt ctgattgagt acaatatctt caagcctgga attgttgtac
      961 ttctcagcaa gtttactcgc atcttcattc ccgagacttt cagctatttt cttttcagtc
     1021 tcatatttca acctgaaata gtatttgttt tcattaataa aatacttctg tacctcgccg
     1081 aagaatactt ccagagaatc agcaaccctc tcattgaaat cactataatt aagcgaatta
     1141 acccattctc ctggtttaat tccggttata tcactcaact ggttaacatg atatttaagg
     1201 atattcagct ttttttcaat cacctcttta tccccggcat ttttgttttt tctctgaagg
     1261 atataattta ttatttcatt tttcagattt ctgatgagtt catctttgta atagttgttc
     1321 tgttctattt cgagattgta cttgaatgtc ggttttatgt acctgttccc tttatatccc
     1381 tccaccatta ttgcctcgta tgcccagcgt gtacccataa ggtcgcctat catcggcacg
     1441 tattctctgt ttgtcagcga tttatgaata tcatcaaatc ttatcattgc tccacccaga
     1501 aggagctgag gaacaagtat cagaggaatg agtatgtaaa tgcttacaac tgttctcata
     1561 cccgatgata tattcagccc cagaagattc ccataacatg ctgtgctaaa aaggataagc
     1621 cattgttgcc agaacatgcc cctgatttcg agtacaaggc tggcaactgc cagaaacagt
     1681 aacgattgca gggctgaaag aacgaaaaga aagcccactt ttgagatcag atagcttaac
     1741 cggctgatat tcagaaactt ttcacgtaca agtattttac ggtccttgaa tatttcctca
     1801 gcactgacca tcagacccag aaacaatgca accacaactg ccatgaaaag aaaaaccgga
     1861 tagtttctat tgtctgcaaa agtgtaacct gaatcgagcg tatatttgga aaaatatccc
     1921 agtatgaaag ctagcagtgg agcctctgca aggttcagta tcatatattg cttgtcggca
     1981 agctttcgca ggaggtttct tttaataaat gttcttatct ggtaggctct tcccggcact
     2041 ttgaagttac ttttcggaag agtgagcttt tgtgggggag aagagcagga aggcatcatc
     2101 ttcctcttgt acttctcgta ccattcaaca ggtgtaacct gtctctcctt tcccggatag
     2161 ccgctatcat ctattgtttt aacctcaata atttcgagga tattatcagt atcgatatta
     2221 ccgcagctcg gacattcgct ttctgccgcg ttggcctgtg aggtttcagt tttgaaatag
     2281 accagggctt caacagggtc gccgtcataa atcatgtacc ctcccctgtc gagaatccac
     2341 agtctgtcga acatcttcag aatgtcggat gagggctgat gaatattgac aatgaccagc
     2401 ttacccgaca gagcctgttt gcgaagcagc atcattacat tatgcgaatc ctgtgaagag
     2461 aggcctgagg ttggctcatc aacgaaaagt accgcaggtt ccctcatcag ctcaagtgct
     2521 atattgagcc ttttacgctg ccctccgctc actttcttgt taagcaaatc tccaacctgc
     2581 agatctttga tttctatcag atcgagatcc gtgagtattt tcactactat ctcatgtaaa
     2641 cgctcctcag tataatcccc gaaacatagc ctggcattga aataaagatt ctgatataca
     2701 gtaagttctt caatcaaaag atcgtcctgc ggcacatatc ctataagacc tttgagatgt
     2761 tcggaatctt tgtaaatatc gtaaccgtta atataaatat gtcctttttc tggctttatt
     2821 ttcccgtgaa gaatattcag cagtgttgtt ttacccactc cgctgctgcc cattatacca
     2881 accaggttcc ccgattcaag cctgaaattc attggcttga tgccgttttc tgagtttttg
     2941 aatctgtatt caatgttaca accttcaaaa actactcttt cgcggaattc atcaatgaga
     3001 aacgccttgt atacctttga ataataaatc ggttcgatac ccggcccttt gatatttaca
     3061 cctctctcca gaaggtaagg cctgcaggca ataatatccc tgcctttgaa ataaaggggc
     3121 agaggaccat catatgtaaa gataagtgaa cctgtactct ctatatgaag cacaaaaaga
     3181 ctccccctga accctcttat gtacatatgc ttccacaggg gataactggc atattcctgt
     3241 tgcgaacgat aatgtggatt atcactttca ataacgagca tgcagtcggg tgatacctta
     3301 tcgtattgcc gccctatcat gaaagcatag gcattatcat attccttcct gcttatgttg
     3361 aaagtcctgg caacaatatc gattatggta ttttcctgtg gcgaaatctg gccatcctca
     3421 aaagtaaatt cgaggagctg cagaaaaaca atcatcctct cttccagaaa gaggctcttt
     3481 tttatctggc ggcagatatt tgttatctgg aatgttatga gtgaatcttc ctcagcaaga
     3541 tcgctccggt taatattctt taactcattt gaataaaact caagattatt ctcaaaaagg
     3601 gaaaaatact cctcttcaag ttcccggtta agatacctcc ggagataacc gcgtaaaatc
     3661 tttttgcccc gtgaagttat tccaccggga tttactgtgg aaattattgc aaagatgtga
     3721 atcagtgcaa gtaatacaga ttccctcatc gaggcaattt ataattgaat ccagctctat
     3781 tttaagttaa tcccctctgc ggctaaaata gtttcatacc gtttctcggg aatcctggct
     3841 cactccggag taaacggaac tctgctttta aattatcttc tccctgatta ccgtaattgc
     3901 ttccgttata tcctttatgt ctttctcagt cagactcgtt gataatcctt cataaacttt
     3961 tttgatgggc tcaagaagct taacaaaatc gcctatcata ggatcatcca gatatggttt
     4021 tgttatatcc agataaagtt caaaggattt cttctgttcg agcagaaccc gtgatatacc
     4081 tgctacatta tacgcagcct cagaaacatg agtggtaaga tacatgcctt caacccacgc
     4141 accccccaca acaagcagag caaggttctg ctgctcattc tcactcagat attgatatgt
     4201 ctcattgaac gcatcggtaa gaatcttaac gagtttatcc ctgttgtcaa aattcacctt
     4261 aatactgtca taaagttttg tatcataaat cctcgacata ttaagggaat ttgccagaac
     4321 tcttatggca tcgagataat catttacatc ctgctgcata ttgtacagcg ttgcataact
     4381 gaggtcggca ccaaaaactc caatatttat ggcctttgct gagctctgaa tatatttctt
     4441 ggcatttgaa ggaggattcg atattcctat tatgtaccct acctcaagtt ctgtgagcat
     4501 cttaatgact tcagcagatg ttggtaaagg atagacattc ttttcaattt cctgtttgat
     4561 tgtttcaacc tgttctttct ctttctgctc ctttacctgc ttctccgcac cagtcttact
     4621 tttacacgat gacatattaa ccagagcaat aacaagtatt actgttattg aaaactgaaa
     4681 aacttttttc ataagtcaat tattttactg tttattaatc tatttaatta ctgatatttt
     4741 ttgtcattaa taccccaatc ctgtacttct tcagagagag aagaaatgtt tttcttctta
     4801 ttccctgcaa gaaaaaaaga ttttaaccgg gcataaaaat aagaaaataa attataaata
     4861 tttagtctta aataagattt taacctgact tctctttttt taatatccca tttggatcag
     4921 cttaaatttg tattttactg ttttcatggt ttgctctttt tctgttttga ctatctttgc
     4981 accctggtaa aaaataatta ttcaggtata aacaaataaa cctgatgcag agagaaaaag
     5041 tcaaaaaagc aaagcttata ctggaagacg gaaccgtttt tgaaggaaat tctttcggag
     5101 ccgatgttcc ggcttcagga gaggtggtgt tcaacactgc tatgacaggt taccctgaaa
     5161 gcctcaccga tccctcatac cggggacaga ttctatgcct cacttatcct cttgtaggga
     5221 actatggagt ccctccggct gaacaagaaa acggacttct taaatacttc gagtcatctt
     5281 cggttcatat ctcgggctta atagtttcac attattcctt tgattacagc cactggaatg
     5341 ccagagaaag cctcggcgac tggcttgcag attatgacat accgggaatt tacggaatcg
     5401 ataccagagc tctcaccaaa cgactgaggg aaaaaggtac aatgctagga aaaatagaga
     5461 ttgaggataa gtgcactgat ttctttgacc cgaaccagat aaacctggta aaggaagtga
     5521 gcacaaaaga aaaaaagatt tacggaaatg gcaggtggaa aattcttctt attgactgcg
     5581 gtgtcaaata caacataata agatccctgc tccggtatga taccacagta atcagggttc
     5641 cgtgggacta cgattatcac aatgaagagt acgacggact attcatttca aatggcccgg
     5701 gtaatccgaa aatgtgtacc gtaacaattg agaatataaa gaaatcactg aaggaagata
     5761 aacccatatt tggaatatgt ctgggcaacc agcttatggc acttgcctca ggtgcagaca
     5821 cctataaact caagtatggt cacaggagtc acaaccagcc ggttatcatg tcaggaacga
     5881 acagggcata tataacttca caaaaccatg gttacgccgt agataacaat acattacctt
     5941 ctggctggga gccttttttc attaacctga acgacatgac caatgaggga atgaaacata
     6001 aatcaggcct ttacttttca acccagtttc atcccgaagc ttcaggtggt ccaaccgata
     6061 cagccttcct tttcgatatg tttatttcaa atattataaa atacaggagc tgaacttcag
     6121 tgcccacctg ttattccttt caagcatact ttagcagttt ctcctcgaga gcagttattg
     6181 gaatggtttt aaattcagcc agcagctttt cagcccttgc tttgatttca tcgttacaaa
     6241 ggtttccgat gctgccctca tgcgaataat cggaaggcat tgttctgaga aaagctccgt
     6301 ctttcagtat gccagataca gccttgtatg catccctgaa aggtatgcca tttctgacca
     6361 tcctgtttac ttcttcggta ctgaaaattg agttatattg gggattgtca agaatattgc
     6421 gccttatctt gatggctccc agcatgtaaa tcaacatttc aatacattcc cctatatcgt
     6481 taaatgcatt aatcatcaaa ctcttaagaa tttgcagatc acgattgtaa cccgacggaa
     6541 ggttatttgt tattatgcta atctgtcccg gcagcgactg aatgaagttt gatcttgccc
     6601 ttattagttc aaatacatca gggttctttt tctggggcat tatgcttgaa ccggttataa
     6661 actcgtcggg aaactcaatg aatttaaatt ctgcagtcat atacaggcat atatccatac
     6721 agaattttga gagggtattg gcaattgaag caagtgcaga tgccacagcc atctcaactt
     6781 ttcccctgtt aatgctggcg taagatgagt tgtatatcat ctcctcaaac tcgaggagtg
     6841 aggttgtgag actgcggttg agtggcagag ttgtaccata tccagcagcc gtaccgagag
     6901 gattcctgtt attcatactc cttgccgctg aaagaagcag aaggtcgtca gtcagggcct
     6961 cagcatagcc accaaaccat aatccaaagg atgacaccat tgcaggctgc atatgggtgt
     7021 accccggaag aagaacttcc ctgtactggt tactcaggga aataagcttc tcaaacaggt
     7081 aacctgtaag ctctgcaatt ctgtcaattt ctgcccgtgt ataaagcctt atgtcgagaa
     7141 gaacctggtc gttgcgcgac cttcctgtat gcaccttccg acccacaggc ccaagcatat
     7201 ccgaaagctc attctcaatc tgcgaatgta tatcctcaac accttcccct atcttaagct
     7261 cacgattcaa atatttctga aaaagagcat gtaat
//
LOCUS       DAOSNB010000110         7233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10353_length_7233_cov_4.484954, whole genome shotgun sequence.
ACCESSION   DAOSNB010000110 DAOSNB010000000
VERSION     DAOSNB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10353_length_7233_cov_4.484954"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1047
                     /locus_tag="PLG42_05775"
     CDS             205..1047
                     /locus_tag="PLG42_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOU30750.1"
                     /translation="MAKNKKGFNLRSFTSFSLVFSTIVMSWSGFILYVAPPGRIANWA
                     DWKLMLFTKAEWQALHTIFSYLFFVLFVIHLFFINWKAFLTYIKSKVKAGLNRKWELA
                     ASTVVFVLFFIGTLKSWPPFGDIMAFGDKVKESWEKSYEAPPVAHMEENTLSDIAANF
                     NGISVDEIVKTLSDSSIIVSDPQLKLKTIAAENKVTPSRIYNILASRYKNEQVTDITA
                     APSGVGKMTVGQVAQRLGKKPEDLIKILKENGIDANEETTMRTIADQMSISPHDLYSL
                     LAGK"
     gene            complement(1009..1815)
                     /locus_tag="PLG42_05780"
     CDS             complement(1009..1815)
                     /locus_tag="PLG42_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOU30751.1"
                     /translation="MNQKNILITGGTSGLGFELAKLFCKANYNVWITGRSLKAGLPEG
                     MKLHFMRTDFADLRQTENVVKQLLGEGISFDVIVNNAGVLGPPLYKTTKDGFEYTFQI
                     NFLAHLLVNNLILSEHVPGKPVTLAIVTSPVYRVVNPDFRIPDGSKYFSYEMYSLTKH
                     YLLLAGDYMKKKYKNQNIRLIKFNPGVFGSGIYRTKQNWFQFLYRIAAPFMRSASRPA
                     GLLYELIHEPHLREDIVYRSKRKAGPASPVLTAEAEKFLLACEEAIKIVR"
     gene            1955..3178
                     /locus_tag="PLG42_05785"
     CDS             1955..3178
                     /locus_tag="PLG42_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOU30752.1"
                     /translation="MPGKLFFNFLLTAVISTSVVSNLYSQDWNQFRGPNRDCKVAGFK
                     APARWPAELKQVWKVTVGTGDATPVLSGNKIYVHTRQGDEETVICLDALTGKEIWKSS
                     YPSPAVTGPSASHPGPRSTPTISGGKIVTLGAAGILSCYDASTGKLLWRKDNPEKLVP
                     QYFTGMSPLIEDGLVIVHLGTRGNGTIYALDLNTGAEKWTVKGDPAYASPSIMRIDGK
                     KHVIINTETSLIAVDLTDGKLQWNIPTPLQQRFYNCVSPCLDGQKMYITGQGTGMKAY
                     VVEKKGNNYEPRELWSNTTTGAKWNTPVLKDGFLYGFTDTRKIYCINASTGETAWLDQ
                     AVHSDFATLVDCGQVFIGLPSTGNLLVFTPSPTSYNESARYRVAETAVYAFPVIAGNN
                     IYVKDAESLTMFRFE"
     gene            complement(3214..4548)
                     /locus_tag="PLG42_05790"
     CDS             complement(3214..4548)
                     /locus_tag="PLG42_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU30753.1"
                     /translation="MSHKFYDIHYHLFDLSHPNLLAFILRDDMITGESVRKVMRKLPL
                     LLKFVPLGAVNFFSGKIAERIKDFIQNDAGKFRNLLSMMENSIEYHFLLADYYIRKEK
                     IFSSGDEKLKFNKIVICPLLIDFGFKNLKNSGCFYDLPPAKPVAGQVADVLKSIRFYY
                     NYDIIPHPVKEGELRLVKSSTSKGDKLFEIYPFLGINPRNYDLLEIKALIDKYFSGYE
                     NDSMPEERYKKLFARLGEVEVNLEDLIFRKKEKENPEYYSYIFAGVKLYPPLGLNPWP
                     EDEPAELDKVKFLLSECARKKIPVTVHCSDGGFKTTPEADYFSNPERWIKILNQSSFK
                     DLRINFAHMGNRASGSQEWLNTIISLAEKFENVYTDFSCLTPEIEDYKLVAGIIEKNM
                     SSRLLFGSDFVINLLWSGSYNEYLKNFLITPYLNTEQKLKVCSANPEKFLFG"
     gene            complement(4555..5832)
                     /locus_tag="PLG42_05795"
     CDS             complement(4555..5832)
                     /locus_tag="PLG42_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOU30754.1"
                     /translation="MARFTGIAGIIIILLLAYLWSNNRKAINLRVVVTGLLLQIALAV
                     FILKVPLGQKIFYWIGLAINKLLDFSQEGAVFIFGDLTKVTSILPEGVVRSGVFLFVL
                     LPTIIFVCVLVAMAYHVGLMQRIVALFARFVHWAMRVSGSEALSNVASAFVGQVEAQI
                     MIRPYLAGMTRSELLASMTGSMACIAGGVMAVYISLGVPASYLLAASLMAAPGALVIS
                     KIVYPETEESETKGIVKLEVKKEHANLMDAIAHGASDGMRVSINVIAMLIGVIALIAL
                     IDALLGYLGQFLAWLGVSLNAIGIDVNHLRLKDIFGAVFSLFAIVMGVPPSESLTVGS
                     LMGTKMVINEFVAYTDLSPLIQQGALSAKSIAIASIALCGFANFSSVAIQIGGIGALA
                     PTRKADLAQLGIKAMLCGTMASYISASIAGILL"
     gene            complement(5913..6716)
                     /locus_tag="PLG42_05800"
     CDS             complement(5913..6716)
                     /locus_tag="PLG42_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30755.1"
                     /translation="MNEKSYNGKNKSRKILIISVSLFISIFAVYYLVLSVVAPAGSIK
                     RINSEYGYKKPEKGGLSDSIFIDSAFIMLNRQKAFYQARIAMASTDSVVLSLNLPDST
                     ATLEINGVTVYTTKILSSKISKTFRKADEYAVTSLFSKPFTILKDYSSIPKEPVVHKV
                     APKDTTEYKPEAPPDTSKIEYANFILETTEGIRLYVYQDTRGNFNAFLNQFLFDLADR
                     TRTSLYTLKCMILFRKPEYKPWIRIRVPLKDARIIYRALPRNGQFAVCR"
     gene            complement(6727..>7233)
                     /locus_tag="PLG42_05805"
     CDS             complement(6727..>7233)
                     /locus_tag="PLG42_05805"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOU30756.1"
                     /translation="QITALKNEVQRLSARYRSMTPGQSYLVINTTDNKFYLYQNRKLI
                     REGYCSSGSYTRLITPEGSREWIFKTPKGKFWIQTKVVKPMWVKPDWAFIEEGLPVPP
                     PDDESRYERGVLGDYAMSIGSGYLIHGTLYKRFIGMPVTHGCIRLNDEDLEAIFNTLN
                     IGSKVYIF"
BASE COUNT         2178 a         1522 c         1456 g         2077 t
ORIGIN      
        1 atggtgtgac ctcgacggta acctgcttat cagcaacgac ccattcgaag gaattaaaat
       61 cgttaacggt aacgtaataa tacctgatcg tcctggaatt gggattgtaa aaatataata
      121 ctttattgtg tcttcctggt gacctgaatc acatataacc tgtccagaaa aaaataattt
      181 tgctgccaga aaaataaatc attcatggca aagaataaaa aaggtttcaa cttaagaagt
      241 tttacaagct tttccctggt cttttcgacg atagtaatgt cgtggtcagg gtttattcta
      301 tatgtcgcac cccccggacg tattgcaaat tgggccgact ggaagctgat gcttttcacc
      361 aaagcagaat ggcaggcact tcacactata ttttcctatc tcttttttgt gctctttgtt
      421 attcacttat tctttatcaa ctggaaagca tttctaactt acattaaaag taaggtaaag
      481 gctggtctta acagaaaatg ggagcttgct gcctcaacag ttgtttttgt gctatttttt
      541 atcgggacat tgaaatcgtg gccacccttt ggagatataa tggctttcgg cgataaagta
      601 aaagagagct gggagaaaag ctatgaggca ccaccagtgg cacatatgga agaaaatact
      661 ctctcagaca ttgctgcaaa ctttaacgga atttctgttg acgaaattgt taaaactctc
      721 agcgacagca gtataattgt aagcgatcct caactaaaac ttaaaacgat tgcagcagaa
      781 aacaaagtta ctccatcaag aatttacaac attctggctt caagatataa gaacgaacag
      841 gttacggata ttactgctgc accgtcaggt gtaggaaaaa tgacagttgg gcaggttgct
      901 caacgtctgg gaaaaaaacc ggaagatctg attaaaatat tgaaggaaaa cggcattgat
      961 gcaaatgagg aaaccaccat gcgaacaata gccgaccaga tgtctatttc acctcacgat
     1021 ctttatagcc tcctcgcagg caagtaaaaa cttctctgcc tcagctgtca gaaccggact
     1081 tgccgggcca gcttttcttt tgctccggta gacaatgtcc tcccgcaaat gtggctcatg
     1141 aataagttca tatagaagac ctgcaggtct tgaggcactg cgcatgaaag gcgctgcaat
     1201 acgataaaga aactggaacc aattctgctt tgtgcggtaa atcccggaac cgaaaacacc
     1261 gggattaaat tttataagtc tgatattctg atttttatac tttttcttca tataatcacc
     1321 tgccagcaaa agataatgct ttgtaagtga atacatttca tatgaaaaat atttacttcc
     1381 gtctggtatc ctgaaatctg gatttacaac cctgtaaacc ggagaagtta caattgcaag
     1441 ggtaactggc tttcccggga catgctcaga aagaataaga ttattgacaa gaagatgtgc
     1501 cagaaaattt atctgaaatg tgtattcaaa tccgtcttta gtggttttgt ataacggagg
     1561 tcctaaaaca ccagcattat ttactataac atcaaaacta atcccctccc ccagcaattg
     1621 cttgacaaca ttttctgtct gacgcaggtc ggcaaaatct gttctcatga aatgaagctt
     1681 cattccctcg ggtaatcctg ccttaaggct tctcccggta atccatacat tgtagttagc
     1741 tttgcagaag agttttgcaa gttcaaaccc taatccagaa gtgccgcctg ttatgagaat
     1801 gtttttctga ttcatcagga aaataaatat agcaggcaca aacatagtca atgtacgcta
     1861 aaaatagcat tattaacatc ttttaagttt aataaaatca acatttatga taaattctta
     1921 aatttggtaa gcattaaaaa ataaattcca taatatgcca ggcaaattat ttttcaattt
     1981 tttactaacc gcagtaatct caacctctgt tgttagtaat ttatattccc aggactggaa
     2041 ccagttcagg ggaccaaacc gcgactgcaa ggttgccggt tttaaggcac ctgcaagatg
     2101 gcctgccgaa ttgaagcagg tatggaaagt aactgttggc actggtgatg ccacacctgt
     2161 tctttcagga aataaaattt atgttcatac ccgccaggga gacgaagaga cagtaatttg
     2221 cctcgatgca ttaaccggaa aagagatatg gaaaagttca tatccatcac ctgccgttac
     2281 cggtccttca gcatcacatc ctggaccaag aagtacgcca acaatctccg gcggaaaaat
     2341 agttacactt ggagctgctg gtatactctc atgttacgat gcctcaacag gaaaactatt
     2401 atggagaaaa gacaatcctg aaaaacttgt tccacagtat tttacaggaa tgtccccact
     2461 tattgaagat ggtctggtaa tagtacatct cgggacaaga ggcaatggaa ctatttatgc
     2521 tcttgacctc aatacaggag ctgaaaaatg gacagttaaa ggcgacccgg cttatgcgtc
     2581 accttccatc atgagaatcg acggcaaaaa acatgtaatt atcaatactg aaaccagcct
     2641 tatagcagtg gaccttactg acggaaagct tcagtggaac attcccacac ctcttcagca
     2701 aagattctat aactgcgtaa gtccctgcct cgacgggcag aaaatgtata ttaccgggca
     2761 gggaaccgga atgaaggcat atgtggtgga gaaaaaaggc aacaactatg agccacgtga
     2821 attgtggagc aacacaacta caggagcaaa atggaacacc ccggttctta aagatggatt
     2881 cctgtatgga tttaccgaca cccgtaaaat atactgcatc aatgcatcaa caggtgaaac
     2941 agcatggctt gaccaggccg ttcacagcga ctttgcaaca cttgttgact gtggccaggt
     3001 gtttatagga ctcccaagca cgggcaatct ccttgtattc actccatcac caacaagtta
     3061 caacgagtct gcaagataca gggttgctga gacagcagta tatgcttttc ctgtaattgc
     3121 aggaaacaac atttatgtaa aggatgcaga aagcctgaca atgttcagat tcgaatagaa
     3181 aatttcaggg tacatctaat cgggggtata aatttacccg aataagaatt tttcaggatt
     3241 agccgaacat actttgagtt tctgctctgt attaagatac ggggttataa ggaagttttt
     3301 cagatactca ttatatgacc cggaccagag caggttaata acaaaatcag acccgaaaag
     3361 aagccgggat gacatgtttt tctcaattat gcctgctaca agtttgtaat cttctatttc
     3421 aggtgtaagg caggaaaaat cggtataaac gttttcaaac ttttcagcaa gagaaataat
     3481 tgtgttcagc cattcctgtg aaccgctggc cctgttcccc atgtgggcaa aatttattct
     3541 tagatctttg aatgagctct ggttcaatat ttttatccac ctctcaggat ttgaaaaata
     3601 atctgcttcc ggtgtggttt taaaaccccc gtcgctgcaa tgtaccgtta ccggtatttt
     3661 cttcctcgca cactcgctca gcagaaactt taccttgtcg agttctgccg gctcatcttc
     3721 tggccatgga tttaagccta gtggaggata aagttttacc ccggcaaaaa tatatgaata
     3781 atattctgga ttttcttttt ccttctttct gaagataaga tcttcgagat taacctcaac
     3841 ttctcctagt ctggcaaaga gctttttata acgttcttcc ggcatactgt cattctcata
     3901 tcctgagaaa tatttatcta tgagtgcttt gatctcaagc aggtcataat tccgcggatt
     3961 gatacccaga aacgggtata tttcaaacag cttatctcct ttactggttg aagacttgac
     4021 taatctaagt tctccctctt taacaggatg tggaataata tcataattat aataaaacct
     4081 tatcgacttc aatacatctg ccacctgccc ggcaacaggt tttgccggag gaaggtcgta
     4141 gaaacaaccg ctgtttttga ggtttttgaa accaaaatct atcagcaatg gacagattac
     4201 aattttgttg aatttcaatt tctcatcccc ggaagaaaat attttttctt tccttatata
     4261 ataatcagca agcaggaaat ggtattcgat tgaattctcc atcatagaga gaaggtttct
     4321 gaattttcct gcatcgtttt gaataaagtc ctttattctc tctgcaattt ttccggagaa
     4381 gaagtttacg gcacccagag gaacaaattt aagaagaaga ggaagtttgc gcattacttt
     4441 tcttactgac tctcccgtta tcatatcgtc gcgcagaatg aatgcgagaa ggttgggatg
     4501 gctcaggtca aaaaggtggt aatggatatc gtaaaacttg tgggacatag gcttttacag
     4561 aagaatgcct gctatgcttg cggagatgta cgatgccatt gttccgcaaa gcattgcctt
     4621 tattccaagc tgtgcgaggt cagctttacg tgttggggct aaggctccta tacctcctat
     4681 ctgtattgcc actgagctga agtttgcaaa cccgcataga gcaatacttg caatggcaat
     4741 tgattttgcg ctaagggcac cctgctggat caggggtgaa aggtcggtat atgcaacaaa
     4801 ctcatttata accattttag tacccatcag tgaacctacc gtaagtgatt ctgagggagg
     4861 cacgcccatt acgatagcaa aaagggagaa tactgctccg aagatatctt taagccggag
     4921 gtggttgacg tcaataccga tagcattgag tgaaactccg agccatgcga gaaactgtcc
     4981 gagatagccg agcagggcat caattaatgc tataagagca ataacaccga taagcattgc
     5041 gatgacgttt attgataccc tcattccgtc gcttgctcca tgggcgatag catccattaa
     5101 gttggcatgt tcttttttaa cttccaattt aactatgccc ttagtttcag attcttctgt
     5161 ctcggggtat acaattttcg aaataaccag tgcacctggt gctgccataa ggcttgccgc
     5221 cagaaggtac gaagcaggaa cgccgagtga tatataaaca gccattacgc ctcctgctat
     5281 gcatgccata ctgcctgtca tggaggctaa cagctccgac cttgtcatac cggcaagata
     5341 tggccgtatc attatctggg cttcaacctg cccgacaaat gcacttgcca cattcgagag
     5401 tgcctcactg ccgcttaccc tcatcgccca gtgaacaaaa cgggcaaaca acgcaacaat
     5461 cctctgcata agtcctacat ggtaagccat cgcaacaaga acacatacga atattattgt
     5521 gggcaggaga acaaaaagaa aaacaccgct acgcacaact ccctcaggaa ggatagatgt
     5581 aacctttgtc aggtctccaa aaataaaaac agccccttcc tgcgaaaaat caagcagttt
     5641 gtttattgcc aatccaatcc agtaaaaaat cttctgccct aaaggtactt taaggatgaa
     5701 cacagcaagg gcaatctgaa gcaacagccc tgttactact acccttaaat tgattgcctt
     5761 acgattgttt gaccacaagt aggcaagcaa cagaataatt ataatacctg caattcctgt
     5821 gaatcgtgcc ataaaaattt ttctgcagag ttcacctcaa acctacataa tttttagaca
     5881 cctgaaacaa aaatgataaa aaaagaagaa atctatctgc agactgcaaa ttgtccgtta
     5941 cgcggaagtg ccctgtatat tattctggca tctttcaggg gaaccctgat tcttatccac
     6001 ggtttatatt caggtttcct gaacaggatc atgcatttca aggtatacaa agatgttctt
     6061 gttctgtcgg caagatcgaa aagaaactgg tttaaaaatg cattgaagtt accccttgta
     6121 tcctgataaa catagagccg gattccctct gtggtttcaa gaataaaatt ggcatattca
     6181 atttttgatg tatcgggcgg ggcttctggc ttgtattcag ttgtgtcttt aggtgccacc
     6241 ttgtgtacta caggctcttt gggaatggat gaatagtcct taagaattgt aaatggtttt
     6301 gaaaacagcg atgttacagc gtattcatca gctttacgga aggttttact gatcttcgaa
     6361 gagagtattt tcgttgtata aacggttaca ccgtttatct caagtgttgc cgtactgtca
     6421 ggcagattca gtgaaagaac tactgagtcg gtagatgcca tggctatcct tgcctggtaa
     6481 aaggctttct gccggttaag cattatgaaa gctgagtcga tgaatataga atcactcagt
     6541 ccaccttttt caggcttttt atatccatat tcgctgttaa ttctttttat gcttccagcg
     6601 ggagcaacaa ctgatagcac aagatagtaa acggcaaaaa ttgaaataaa taaggaaaca
     6661 gatattatta aaatcttacg tgatttattt tttccattgt atgatttctc attcatattc
     6721 agcttgttaa aagatataaa ctttcgagcc tatattcagg gtattgaaga tggcttcgag
     6781 gtcctcatca ttaagcctga tgcagccatg agtaactggc atcccgatga accttttata
     6841 tagtgtgcca tgtatgagat aaccgctgcc tatactcata gcataatctc ccagaacgcc
     6901 tctttcgtac ctcgattcat catctggcgg tggaacgggt agtccttcct caataaaggc
     6961 ccagtcgggt tttacccaca tgggtttgac cactttcgtc tgaatccaga atttaccttt
     7021 aggggtttta aatatccatt ctcttgagcc ttctggtgtt ataagacgtg tgtaactccc
     7081 cgaactgcaa tatccctccc ttatcagttt tctgttctga tagaggtaaa acttattatc
     7141 agtggtatta ataaccagat atgattgccc gggtgtcatg gaacggtagc gcgcagaaag
     7201 cctttgtact tcatttttaa gtgcggtaat ctg
//
LOCUS       DAOSNB010000111         7230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10357_length_7230_cov_3.814913, whole genome shotgun sequence.
ACCESSION   DAOSNB010000111 DAOSNB010000000
VERSION     DAOSNB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10357_length_7230_cov_3.814913"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLG42_05810"
     CDS             <1..608
                     /locus_tag="PLG42_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOU30757.1"
                     /translation="LMGAGRGTSNMIFMTFGTGMGGGLILSGRLYSGTNNLAGEVGHI
                     RLDKLGPVGYGKAGSFEGFCSGGGIAQLAQSVVREYFYSNRKVNFCPSVEMIDEIDTR
                     TVALAARNGDPVALEIIKTAAEYLGYGLAILIDILNPECIVIGSIFTRNEDLFRPHME
                     RILQQEALAPALAVCKIKPAELGESIGDYASLCVALYRDMF"
     gene            647..3208
                     /locus_tag="PLG42_05815"
     CDS             647..3208
                     /locus_tag="PLG42_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOU30758.1"
                     /translation="MKKLSAKQKNQITRRVFLSNTIAGTAVAFFYPLPELITGDNQQT
                     VVKWPEDAGKFRFYLTGHAHIDPVWLWPWFEGVAVVHSTFRSALDWMKRNPEFCFTAS
                     SAQFYEWVAENDPKMLGEIRAKVAEGRWCIAGGWWVEPDVNIPCGEAMVRQGLYGQRT
                     FQKLLGRKAKVAFNPDSFGHAGSLPQIFLLQGMENYIFMRPQPKEKTLPGNIFWWEGI
                     DGTRILTYRIPISYNDTSPVQNRVSQILNLYRNEPVSSFLGFFGAGDHGGGVTKENID
                     SIEELKKEKGAPTVLFSTPERWFSEIRKLNSKFPVVKDDLQYHAVGCYTAESGIKKGN
                     RQAETALITAEKICSIGSIIWGAVYPAKEFEQAWKRVLFLQFHDSLAGTSLFEHSQTA
                     REGFGFATDIAEQAKYLALQKLEWQIPAKDPASQYFIAFNPHAWEVKTNVEYDLSWQE
                     QKASAVEDENGNPLLFQWTAGSSEAGSRRKLVVKTSLPPFGYRQIRVKQATTPTFSTN
                     VKAEGNILENEYFRITFSESGTVSLFDKENDIELLDGGNGCSAVVIDDPSDTWSHDIK
                     TFNNETGSFGNATIKVLETGPLRGKTRVTSTWGSSTLIIDWSLSAGSRKIEANVTLNW
                     NERLKMLKLSFPVNVDKPIATTEIPYGAIERQKDGMEVPGQRWIDITGMNKGSLYGLT
                     VFNDAKYGYSFNGSDMRISVVRSAVYAHHDPKKLDPQREYLWMDQGIQTFRILLMPHK
                     GKWQENDIPRIAEEFSTPPLVIYQGIHDGKMPGSGSFITADSPGLVISAIKKSEEGND
                     IVLRVVETLGLPVLGGISLKFSGMGWIGSFRPYEIKTLLINVKAGTIREVNLLEE"
     gene            complement(3217..4053)
                     /locus_tag="PLG42_05820"
     CDS             complement(3217..4053)
                     /locus_tag="PLG42_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30759.1"
                     /translation="MAILIPVFGLILFLMFSSGLNDTIQIAIFVFTALILLMGILLFY
                     KITIYIDERQVYFKMGCGLISKRYNLDEIKNCKPVRNPLLYGIGLRKIPGGWLFNVSG
                     LDAIELEFKNSKSIVRIGTDKPEEVAAVINRLIKSDLHVPFKKEKEYSAVILAIIIII
                     FSVLLPASLLVAGSKETEAFISDNQLIIKGMYGINIKLTDIVRVDTFPVLPGIKKRDN
                     GYASGSTLKGRFTLKDGSKARLFVKKDKPPFINIKTDKLNIWLNFTNPGNTRRLYTEL
                     NK"
     gene            complement(4197..4844)
                     /locus_tag="PLG42_05825"
     CDS             complement(4197..4844)
                     /locus_tag="PLG42_05825"
                     /inference="COORDINATES: protein motif:HMM:NF020106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30760.1"
                     /translation="MKRNILIMVLFTIGVAAGKTTILNSSSEWIDLNQSLKTEHITYN
                     NIVSFCFEKGTMVTMADGSSKPIEKVTPGDKILSVNTLEMSVENDIVEKVDSAICNNF
                     VKIFFDDLTMNVNTDDHLYYVKGKGWCSVNPESTFRKYKIRAGILKPGDDCYKILNGR
                     LINVKISTIQRIPGRHTGFSIVKLNKNRSYFANGKLVSIEEVETIKEQTRTKPKK"
     gene            complement(4925..5800)
                     /locus_tag="PLG42_05830"
     CDS             complement(4925..5800)
                     /locus_tag="PLG42_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30761.1"
                     /translation="MFLILTAGCEKIENRNFYKYPFVISIFEGSNIYDLMPRPNNPVF
                     TGDGVKDAQVSFVAYPFLIKKGDKWYMFFEAYQCAIHKGVIAYAESEDDCYTWKYGKI
                     ILDKPWHQSFPCVFEYNSEYYMVPESKHSGSTWLHKAINFPEEWSDGKKILNIPLVDP
                     AIFRKDGRWYMFGLDRHNLLLFYSNNHETGWKEHPASPIIIGDKKYRRPGGGVYFNGK
                     DYYRIAQDCESDYGVAIHIFKIIELNQTKYREIPVPDNYNLHAGAEEWAKHGIHTLNI
                     QQLDTNRIIAVVDGY"
     gene            complement(5938..>7230)
                     /locus_tag="PLG42_05835"
     CDS             complement(5938..>7230)
                     /locus_tag="PLG42_05835"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOU30762.1"
                     /translation="KKEWSESFILTPDWTEETHGNTISPNYSIVFPEDKVQRFDIIIS
                     SKEWNKMQGDLRANVRFVNNQLVVTPGWVPVWVPCSFRFNGKEWYKVGIRYKGNSSLK
                     ECIRRNISKYSFKLDFDEFENNYPAIKNQRFYGFKQLNLSNGFDDISLMREKSAADLF
                     REFGIPSSRCAFCEIWIDYGEGSKYFGLYTLIEEVEETVITNQFEGGGNLYKPEGSAA
                     TFAYGTFNIFQMFRQNNLENADYSDVKSLYDILHSPLRLNDQTEWKSRLEAVFDVPHF
                     LKWLAANTVMQNWDTYGKTQHNYYLYNNPKTKKLTWIPWDNNEALRPGKEGGALSLPL
                     NEVSDKWPLIRFLINDTCYFSMYKSYVKEFIACPFSVTQFTERVNLQADLIRKFATSE
                     RKGCTFLPSPFAFEQGVLDLQNHVQARVNAAIEFTGVK"
BASE COUNT         2154 a         1392 c         1478 g         2206 t
ORIGIN      
        1 ggcttatggg ggctggcaga ggcacttcga acatgatatt catgaccttc ggaacaggga
       61 tgggcggcgg acttatcctt agtggccggt tatacagcgg aacaaataac cttgcaggtg
      121 aagttggaca tatccgcctt gataaattgg ggccagtagg ttacggtaaa gcaggttctt
      181 ttgaaggctt ctgcagcgga ggaggcattg cacagctcgc tcaatctgtg gtaagagaat
      241 atttttatag caacagaaag gttaatttct gcccatcggt agaaatgatt gatgaaattg
      301 ataccagaac ggtggctctt gctgcacgga atggagaccc cgtagctctt gagataataa
      361 aaacagctgc agaatacctt ggttacggcc ttgctattct tattgacatt ttaaacccag
      421 aatgcatcgt tatcggcagt atttttacac gaaatgagga tcttttcaga cctcatatgg
      481 aaaggattct gcaacaggaa gctcttgcac cagcacttgc ggtatgtaaa atcaaacccg
      541 cagagcttgg agaatcaatt ggcgattatg cttcattgtg cgttgcactc tacagggata
      601 tgttctgact agcttttttt aacaagatta aaaaaataat taccagatga agaagctttc
      661 tgcaaaacaa aaaaatcaga ttaccaggcg tgtttttctc agtaacacaa ttgccggtac
      721 agcagtggca tttttctatc cgctgcctga gctaattacg ggagataatc agcagactgt
      781 cgttaagtgg cctgaagatg ccggtaaatt caggttctac ctcacagggc atgcacatat
      841 cgaccctgta tggttgtggc catggtttga aggtgtagca gttgtccaca gcaccttccg
      901 gtcggcactc gactggatga aaagaaatcc tgagttttgc tttactgcaa gctctgcaca
      961 gttttatgaa tgggtggcgg agaacgaccc gaagatgctc ggtgagatac gtgcaaaagt
     1021 tgcagaaggc cgctggtgta tcgccggagg gtggtgggtg gaacccgatg tcaacatacc
     1081 gtgcggcgaa gcaatggtga gacagggatt atatggccag cgtacttttc agaaacttct
     1141 cgggagaaaa gcaaaagttg cttttaaccc cgactctttt gggcatgccg gctctcttcc
     1201 tcagatattc ctgctacagg gtatggaaaa ctatattttc atgaggcctc agccaaagga
     1261 gaaaaccctt ccgggtaata tattctggtg ggaaggcatt gatggaacca gaatattaac
     1321 atatagaata ccaataagtt ataatgatac cagtccagtc cagaaccggg tcagtcagat
     1381 ccttaatcta tacagaaacg agcctgtaag tagtttcctc ggcttcttcg gagcaggtga
     1441 ccatggggga ggggtaacta aagaaaatat tgactcaatt gaggaactta aaaaggaaaa
     1501 aggtgctcct acagttctgt tcagcactcc agaacgatgg ttcagtgaaa tacgtaagct
     1561 gaacagcaaa ttccctgtag taaaagacga tttgcagtat catgcagtag gctgctatac
     1621 agctgagtcg ggaataaaaa agggaaaccg tcaggctgaa actgcactga ttacagctga
     1681 aaaaatttgt agcatcggtt caataatatg gggtgctgtt tatccagcta aagaatttga
     1741 gcaggcatgg aaaagagtac tctttctcca gtttcatgat agccttgcag gcacatcact
     1801 ttttgagcat tcacaaacag ccagagaggg attcggattt gcaactgaca ttgccgaaca
     1861 ggcaaaatat cttgcattac aaaaacttga atggcagata cctgcaaaag accctgcttc
     1921 acaatacttt atagctttca atccacacgc atgggaggtg aaaactaatg ttgaatatga
     1981 cctcagctgg caggaacaaa aagcatcggc agtggaagat gaaaacggta atccattact
     2041 gtttcaatgg acagcaggtt cttccgaagc aggcagcaga agaaaactgg tggtaaaaac
     2101 ttcgctgcct ccattcggtt acagacagat acgagttaag caggctacca caccaacatt
     2161 cagtaccaat gttaaagcag aaggcaatat tcttgaaaat gaatatttca ggataacatt
     2221 ttcggaatcg ggtacggtaa gcctttttga caaagaaaat gacattgaat tgttagatgg
     2281 tggaaatggt tgcagcgcag ttgttattga tgatccgagc gatacatgga gtcatgacat
     2341 aaaaacattt aacaacgaaa ccggttcttt cggtaatgcg acaataaaag ttctggaaac
     2401 aggacctctt aggggtaaaa caagagtaac aagtacctgg ggtagttcaa ctctcattat
     2461 tgactggtca ctgagtgcgg gctcgaggaa aatagaagca aatgttacgc tcaactggaa
     2521 cgagcggctg aaaatgctga aactttcctt tccggttaat gttgataaac caatcgctac
     2581 taccgagatt ccttacggtg caatagaaag acaaaaagac gggatggaag tcccgggtca
     2641 gcgttggatt gatattacag ggatgaataa aggcagtttg tatggactca ctgtattcaa
     2701 cgatgcaaaa tacggataca gctttaacgg tagtgatatg aggatttcag ttgtgaggtc
     2761 tgccgtttat gcccatcacg atccgaaaaa gcttgatcct caaagggaat atttatggat
     2821 ggatcagggc attcagacat tcaggatttt gctgatgcct cacaagggaa aatggcagga
     2881 gaacgacatt cccagaattg cagaagaatt ctcaactcct cctctggtaa tatatcaggg
     2941 aatacatgat ggaaaaatgc caggaagtgg ctctttcata acagctgatt ctccaggtct
     3001 tgttatttcg gcaataaaaa aatcagaaga gggtaatgat attgttctca gagttgtaga
     3061 aacacttggt ttacctgttt tgggaggaat aagcctgaag ttttctggaa tgggatggat
     3121 cggttcattc agaccttacg aaattaaaac tttgcttatc aatgttaagg ctggtacaat
     3181 aagagaagtg aaccttcttg aggaatgaag gtgtttttat ttatttaatt cagtgtataa
     3241 ccttcttgta ttgccaggat tggtaaaatt cagccagatg ttcagtttat ctgttttgat
     3301 atttataaac ggtggcttat ctttctttac aaacaacctg gcttttgagc catctttaag
     3361 agtgaacctt ccttttaaag tacttcctga agcataaccg ttatctcttt tttttatacc
     3421 tggaagcaca ggaaaagtgt caactctcac aatatcagta agtttgatgt taattccata
     3481 cattcctttt attatgagct ggttgtcaga aataaatgct tctgtttcct tacttcctgc
     3541 caccagtagt gaggctggta aaagaacgct gaatataata attataatgg caaggatgac
     3601 tgctgaatat tctttttctt ttttgaacgg tacatgtaag tcacttttta taagtctgtt
     3661 aataactgca gccacctctt cgggcttatc tgtgccaata cgtacaattg acttgctgtt
     3721 tttgaactca agttcaatgg catccagtcc cgatacatta aacagccatc cacctggtat
     3781 ctttctcagt ccgattccgt aaagcaacgg atttcttacg ggcttgcaat tttttatttc
     3841 atcaaggtta tatcgttttg agataagacc acatcccatt ttgaaataaa cctgcctttc
     3901 atcaatgtaa atggtaattt tatagaaaag aagaatgccc atcagcaaaa ttaatgctgt
     3961 gaaaacaaaa atagctattt gtatagtgtc gttaagacct gatgaaaaca tgagaaatag
     4021 tatcaaaccg aaaaccggaa tgagaattgc catagaaaat gtgccaggtt gggtgaatct
     4081 gtattgttgc ataagatata gaattattct tatgaacaaa tattaaataa agatttataa
     4141 ttacgctaac gctgagcaat aaaaatgctt ataaaagtaa tgttcctatt atgcaattat
     4201 ttttttggtt tagttcgagt ttgttcttta attgtttcaa cttcttcgat tgatacgagt
     4261 ttgccattgg caaagtaact gcggtttttg tttagtttaa ctatgctgaa tcctgtatgt
     4321 ctgccaggaa ttctctgtat tgtgctgatt tttacattta tgagtctgcc gttcagtatt
     4381 ttgtaacagt catcacccgg tttcagaatg ccagcacgta ttttatactt cctgaaagtt
     4441 gactcaggat taaccgaaca ccatccttta cctttcacat aataaaggtg atcatcagta
     4501 ttaacattca ttgtgaggtc atcaaaaaat attttaacaa aattattgca gatggctgag
     4561 tcaacttttt caacaatatc attttcaact gacatctcca gtgtattgac tgataaaatt
     4621 ttgtcaccag gagtaacttt ttctataggt ttcgaacttc catcggccat tgttaccatc
     4681 gttccctttt caaaacaaaa tgatacaata ttgttgtaag taatgtgctc cgttttcagg
     4741 ctctggttaa gatcaatcca ctctgaagat gagtttagta ttgttgtttt cccggcagct
     4801 actccaattg taaaaaggac cataattaat atgtttcttt tcataagcaa tcatattttg
     4861 tttttcaaaa ttgccggatt gccccggcag ggtgactttg agccggagac ttgccggcta
     4921 ttattcagta accatctaca actgcaataa ttctatttgt atcaagttgc tgaatattga
     4981 gtgtatgaat gccatgcttt gcccactctt cagcccctgc atgaaggttg taattatcag
     5041 ggactggtat ctccctgtat tttgtttggt taagttcaat gattttaaaa atatgaatag
     5101 ctacaccgta gtccgattca cagtcctggg caatcctgta ataatccttt ccgttgaaat
     5161 agactccccc gccagggcgt ctgtatttct tatctccaat tattatcgga ctggctggat
     5221 gttctttcca tccggtttca tgattattgg aataaaaaag aagcaaattg tgcctgtcaa
     5281 ggccaaacat ataccatctt ccatcctttc tgaagatagc cggatctacc agggggatat
     5341 taagtatttt ctttccatct gaccattctt caggaaaatt aattgctttg tgcaaccatg
     5401 ttgatccgga atgttttgac tcaggtacca tgtagtattc actgttatat tcaaaaacgc
     5461 acgggaatga ctggtgccat ggtttgtcaa gaattatttt gccatacttc catgtataac
     5521 agtcgtcttc actttcagca taggcaatta cacctttgtg aattgcgcac tgatacgctt
     5581 caaaaaacat gtaccattta tctccctttt taatcaaaaa aggataagcc acgaaactaa
     5641 cctgtgcatc ctttactcca tctcctgtaa atacaggatt attgggtcgt ggcataagat
     5701 cgtaaatgtt tgagccctca aaaattgaaa taacgaaagg atatttataa aaatttctgt
     5761 tttcaatttt ttcgcatcct gcagtaagaa tcaaaaacaa tttcagaaaa acaattaaaa
     5821 taaaacattt atcaggaatt atattttttt gaaagataag catttgaata atgatctggt
     5881 tttagatgac agttcttaaa acacccgcat taaatttata aaatgttcac taaactatta
     5941 ttttactcct gtaaactcaa ttgcagcatt tactcttgcc tggacatgat tctggaggtc
     6001 gagaacgccc tgttcgaatg caaatgggga ggggagaaag gtgcagccct ttctttcaga
     6061 agttgcaaat tttctgataa gatctgcctg taggttaact ctttctgtaa attgtgtaac
     6121 agagaaagga caggcaataa attctttaac atacgatttg tacatagaaa agtagcaggt
     6181 gtcatttatc aggaagcgta tcagtggcca tttgtcgctt acttcattta atggcaggct
     6241 gagggctcct ccttcttttc cgggccttaa tgcttcgtta ttatcccatg gtatccatgt
     6301 aagttttttt gttttcggat tattgtacaa ataatagtta tgctgcgtct ttccatatgt
     6361 atcccagttt tgcattaccg tgttggctgc aagccatttc agaaaatgcg gaacatcaaa
     6421 tactgcttca agccttgatt tccattcggt ttgatcattg agtcgcaggg gggaatgaag
     6481 aatatcatat aacgatttga catcagaata atcggcattt tcaagattat tctgcctgaa
     6541 catttgaaat atattgaagg ttccgtatgc aaaagttgct gctgatcctt cgggtttata
     6601 gagattgcct ccaccttcaa actgatttgt tattactgtt tcctctactt cttctatcag
     6661 ggtgtaaagg ccaaaatatt ttgagccttc accataatca atccatattt cacagaacgc
     6721 acacctggac gatggaatgc caaattccct gaagaggtca gccgcactct tttccctcat
     6781 caggcttata tcatcaaaac cgttcgacag attcagttgc ttgaagccat aaaatctctg
     6841 attctttatt gccggataat tattctcaaa ctcgtcaaaa tcaagtttga aagagtattt
     6901 gcttatattt cttctaatac attctttcaa acttgaattt cccttatatc gtattccaac
     6961 cttgtaccat tccttcccgt tgaacctgaa ggagcacggc acccataccg gcacccagcc
     7021 cggagtgact accagctgat tatttacaaa acgtacattt gcacgaaggt caccctgcat
     7081 tttattccac tcttttgacg aaataattat gtcaaaacgc tgtactttat cctcaggaaa
     7141 aacgatactg tagttgggtg aaatagtatt accgtgtgtt tcttctgtcc agtcgggtgt
     7201 taagataaat gattctgacc actctttttt
//
LOCUS       DAOSNB010000112         7120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10564_length_7120_cov_3.977636, whole genome shotgun sequence.
ACCESSION   DAOSNB010000112 DAOSNB010000000
VERSION     DAOSNB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10564_length_7120_cov_3.977636"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..3136)
                     /locus_tag="PLG42_05840"
     CDS             complement(35..3136)
                     /locus_tag="PLG42_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU30763.1"
                     /translation="MKLTLESIKRPAGTIILMIVIAVLGIAGFTSLPVNMLPDITYPM
                     VKVYVYWPGATPEQMENEVAMVIERKMATVDNLDYIESTSEEGVYTLLVNFDYSVDRD
                     VAYQDVLAKMGLVKKELPQEILEPVVFKADPSQLPVVELLVSSDNMSLTQLRTWIENE
                     FQEQFASVKGSAGTALSGGTMREIRIHVDPVKLQGYNITLSQITERLKNENIDMVGGR
                     MLTPTRDYIVRTYGEFKSISEIEDLVIRKSSTDAQILLKDVAVVEDLYALQKIRTKYN
                     GHEGVRISIFKQAEANAVEVSDEIARRVKELKEELPETTRLEIIYDQAEYVRLATNGV
                     RDAVLLAAIFVVLITALFLSGWRRVLVLILSLPVTILGTFFLMWLLGYSVNIFTLGGL
                     VVAMTVVLDNSVVILENITRIQETEPESSDQVTLGSLQVTGAIITSTVTFLSLFIPFL
                     LVPGLSSLLFRELVLTVAIIIFFSMVVSLTVTPMLMALFYPASKRIIPKHGIISRGAD
                     YFIKGIIFIYKPILKYSLKFRWIVLLAFLLLLIPGYSFLKRTGSEFLPKADDGLININ
                     IVMPTGTSMEETDRALSQAEEVIKKQEFIKGYATLAGGKVWGLVTTESSFLGELNVEL
                     VPASQRPMNTDEYVEMLRPQIMKAVKVPGAVVKVMHAKMKGIRQIGQFDIEVMISAPR
                     SVSMQEIYNVASSVRNEVREYEFLTGLDISLQLTKPEYQIHVDRQKAFDLGLSYSDIG
                     NTVRTMVGGTVPTRYKEGAYYYPVRVVMDEREIQSSEALENLYVYSAKGVKIPLSAVA
                     EVVKTTGPVQIDRQNQDRLIKVTANVAGISVGEATNIIKQKLDNFNLPSGYRINYGGQ
                     SQMLSENMRQIAIILLFALFLGYAVLVLYFESFIKPLIIIIRIPLSLAGISYALYITG
                     TPISVTALIGIILLTGMEINNGVLLLTFIDELREKGMDIKEAIKQAALIRLRPILITN
                     LNSLAGLLPLALTMGDGTEMLKPMAIVVIGGVLFGLLLVFIFLPVTYMIIYGKRPKMI
                     KTL"
     gene            complement(3136..4185)
                     /locus_tag="PLG42_05845"
     CDS             complement(3136..4185)
                     /locus_tag="PLG42_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU30764.1"
                     /translation="MNKIKITILVSLSVLLALTSCDRKKGENTVQSAAKKAPPLVRVQ
                     SASSTTMISYIDVTGTIEANISTDVKSPANGIIEQLNYRENQRTEKDKIIAVINPDER
                     LSLISANQLAIEELEKMILSADKNSGEYDSLAKDLEKAKGHLRFARDMYQPVPVICPM
                     NGVVTSRWVEVGSQVAARERIVTISDMNSLVIKAEVNEKYFEAIAPGRKFPVVLNAYP
                     SDSLTGIVSLVYPSVSSESRAVKFDLKLQGFNKKLLPGMMAHLRIPVYRNDKAIIVND
                     DAILSSPDGKRFLFVVDNDTIARRRIVKTGVTVNRMTEITEGLNQGEMIVVAGQETLK
                     DNMKVSLPAPQKNSK"
     gene            complement(4197..5510)
                     /locus_tag="PLG42_05850"
     CDS             complement(4197..5510)
                     /locus_tag="PLG42_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU30765.1"
                     /translation="MKTKAVLIYILLLICIYSAAGQDPGYVYNLKQLIDSALQNNYLI
                     KANEKQSAVKQSDIELLRLTYQPTISASATASVWKFLLPNKQRLLGNSLTDVYTDISV
                     YQTIYEWGENKVRKSAIKDEILLNEEIKRQLRSTIIWGVEDTYYEILKAESEVTVHHN
                     TLYQLRTHLRYAEALYKIGKVSGVDVLKINVQISVEEKALQKSVNSVLAHQIKLGRLC
                     FPERPHPLLIEGHIDTISTSNLSRILIPDSLYKQTLDNHPSIKAANLKITLEDKQKEI
                     ARLLNRPELYSYGNGTWEHGYIPFGKNFNYNIGVGIRYTIPYFGGSGYKTKMLQSDLR
                     VEQINDEKAQLFVDLLREIDITLNEIKDIKEEISNNKKIIDLARETLENASVLYQGGQ
                     GTIIDVLDAQTILSEATINYQKSTIAFLQAFSKLRYLTGNDNNPY"
     gene            complement(5773..6183)
                     /locus_tag="PLG42_05855"
     CDS             complement(5773..6183)
                     /locus_tag="PLG42_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU30766.1"
                     /translation="MITPKTPSFQSFNIFGVEHISPENALEELDKGLAIFVDVREESE
                     FMIEFISLNDVFNFPMSAIMDNLKNIPDDKPIIVFCQGGVRSTKVANMLNRNGFVNSV
                     NLDGGLKTWKAKGLPFESILPLACSSCSGSCSCS"
     gene            complement(6186..6590)
                     /locus_tag="PLG42_05860"
     CDS             complement(6186..6590)
                     /locus_tag="PLG42_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU30767.1"
                     /translation="MEKIRKSLLLFAALATIILSTDCSGQTKKADKNNTGSEKKTYKV
                     TFIELGSVRCIPCQQMQPIMKSIQEKYGDQVKVVFYDVWTEAGAPYAQKYRIQAIPTQ
                     VFLDENGSEYFRHVGYFPEEELVKVLQQKGVK"
     gene            complement(6590..6952)
                     /locus_tag="PLG42_05865"
     CDS             complement(6590..6952)
                     /locus_tag="PLG42_05865"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOU30768.1"
                     /translation="MENSQKIQIVSCSGASNTGEFADKVARKLDESGEANMVCLAKVA
                     VGDQSLINKLKTQQDKKIVVLDGCAINCAMKILGKENITITNIIHVNTTDFGIIKGKT
                     PVTDEKINEIVEYIKKIA"
BASE COUNT         1937 a         1538 c         1361 g         2284 t
ORIGIN      
        1 ttatttcttt caattatgag attaagatac gtttttataa ggtttttatc atctttggtc
       61 ttttaccata aatgatcatg taggtcacag ggaggaatat aaagacgagc aacaggccaa
      121 ataacacacc tccgatcacc actattgcca ttggtttaag catttccgtt ccgtctccca
      181 ttgtaagagc cagtggaagt aagccagcca gactgtttaa gttggtaata agaattggcc
      241 tcaacctaat tagtgcagcc tgttttattg cttccttaat atccattcct ttttcacgaa
      301 gttcatcaat aaatgtgagc aggaggaccc cgttgttgat ctccattcct gtaagcaata
      361 ttattccgat gagtgctgtc acgctgatgg gtgttcccgt aatataaagt gcataggaga
      421 tacctgccag cgataaaggt attcttataa ttattatcag gggtttgata aaactctcaa
      481 aatagaggac caataccgca taaccaagga atagtgcaaa cagcaggatt atggctatct
      541 gtctcatgtt ctcggaaagc atctgggact ggcccccata gttgattctg tatcctgaag
      601 gcaggttgaa attatcaagt ttctgcttta taatatttgt tgcttcacca acacttattc
      661 ccgcaacgtt tgcagttact ttaatgagac ggtcctgatt ctgcctgtcg atctgaacag
      721 gacctgtggt cttaaccact tccgcaacag cagacagagg tatttttacc cctttagccg
      781 aataaacata aagattttca agtgcttctg acgactgaat ctctctttca tccataacga
      841 ccctgaccgg gtaatagtat gcaccctctt tatatcgagt gggaacggta cctccaacca
      901 ttgtcctgac tgtattcccg atatcgctgt agctgagacc gaggtcaaat gctttttgcc
      961 ggtcaacatg tatttggtat tccggctttg taagctgaag agagatatca agtcctgtaa
     1021 gaaattcata ttctctgact tcatttctta ccgacgaggc aacgttgtat atttcctgca
     1081 tggagacgct tcggggcgct gagatcatga cttctatgtc aaactggccg atctgtcgga
     1141 tacctttcat tttggcatgc atcaccttta ctacggctcc cggtaccttt acggctttca
     1201 taatctgcgg tcgcaacatc tccacatatt cgtcagtatt catgggcctt tgtgaagcag
     1261 gaaccagttc aacattaagc tctcccagga agctgctttc agtggttaca agtccccata
     1321 ccttgcctcc ggccagtgtg gcatatcctt taataaattc ctgttttttt attacctcct
     1381 ctgcctggct tagcgctctg tcagtctctt ccattgaagt tcctgtcggc ataacgatat
     1441 tgatgtttat caggccatca tcggcttttg gcagaaactc tgatcctgtt cttttcagaa
     1501 aactatatcc gggaataaga agaagcagga atgcaagcag aacaatccac ctgaacttca
     1561 gggaatactt aagtattggt ttgtaaataa atataatacc ttttatgaaa tagtctgctc
     1621 cccgtgagat aatgccatgt ttaggtatta tccttttcga agcaggataa aacagcgcca
     1681 tcagcattgg tgtcacggta agtgatacca ccattgaaaa gaatattatt atcgcaacgg
     1741 taagaaccag ttcacgaaaa agaagtgatg ataatcccgg aaccagaagg aaggggataa
     1801 aaagagaaag gaatgtaacg gttgacgtaa taattgctcc ggtaacctgt agtgaaccta
     1861 aggtaacctg atctgatgat tcaggttcgg tttcctgtat tcttgtaata ttttcgagta
     1921 ttaccacact gttgtcaagg accactgtca tggcaactac cagcccgccc agtgtgaaaa
     1981 tattaacaga atagcccagc agccacataa gaaagaatgt tccgaggatc gtaaccggaa
     2041 ggctcagtat cagaaccagc acacgcctcc atcccgacag gaagagtgca gtgatcagta
     2101 cgacaaatat ggcggccagt aatactgcat ccctgacacc atttgtagcg agtcttacat
     2161 attctgcctg atcataaatt atctccagcc ttgtcgtttc aggtaattct tcttttaatt
     2221 ccttcactct ccgggcaatc tcatcagata cttcaactgc atttgcttcc gcctgcttga
     2281 aaattgaaat ccggactcct tcatggccat tatatttggt acggattttc tgtaaggcat
     2341 aaagatcttc cactactgca acatccttca ataaaatctg ggcatcagtt gatgatttac
     2401 gaataacaag gtcctcaatt tcagagatcg acttaaattc cccataggtt ctgacaatat
     2461 aatcgcgtgt gggggtaagc attcttcctc ccaccatgtc aatattttca tttttaagtc
     2521 tctcggtgat ctgtgataaa gtaatattat atccctgcag ttttactggg tcgacatgta
     2581 ttcttatctc acgcatagtg ccgcctgaaa gtgcagttcc tgcggaaccc ttaacagagg
     2641 caaactgctc ctgaaattca ttctcaatcc aggtcctgag ctgtgtcaga ctcatattat
     2701 ctgatgaaac aagcagttca acaacaggca gctgtgaggg atcagcttta aaaacaaccg
     2761 gttcaaggat ctcctggggc agttcttttt tgacgagtcc catcttagca agaacatcct
     2821 ggtatgcaac gtccctgtcg acactgtaat caaaattcac cagaagagta tatactcctt
     2881 cttcagatgt agattcaatg taatcaaggt tgtcgacagt agccattttt cgctcaatta
     2941 ccatggccac ttcattttcc atttgctccg gtgttgcccc aggccagtaa acatagactt
     3001 tcaccatagg gtatgtgatg tcgggaagca tattcaccgg aaggcttgta aatcccgcta
     3061 tgccgaggac cgctattacg atcatcagta taatggtacc ggcaggcctt tttatcgatt
     3121 caagagtcag tttcattact tactgttttt ttgtggtgcg ggcagggaga ccttcatatt
     3181 atcttttaaa gtttcctggc cagccaccac aatcatttct ccctggttca ggccttctgt
     3241 aatttctgtc attctgttca cagtaacacc agtttttaca attcgccgac gggcaatggt
     3301 gtcattgtca actacaaaca ggaatctctt gccgtcaggt gaagagagta tagcatcatc
     3361 attgacaatt attgccttat cgtttctgta aacaggaatt ctaaggtgtg ccatcattcc
     3421 cggtaaaagt ttcttgttga atccctgcag cttcagatca aacttaactg ctctgctttc
     3481 ggaagagaca gaagggtaaa ccagagaaac aatacctgtc agggaatcgg aagggtaggc
     3541 attcaatact acaggaaatt tacggcctgg agcaattgcc tcaaaatatt tttcattcac
     3601 ttcagccttg attaccaggc tgttcatatc acttatggtg actattcttt cccttgctgc
     3661 cacctggctc ccaacctcaa cccagcgcga tgtaacgact ccgttcatcg ggcagattac
     3721 gggtaccggc tgatacatat ccctggcaaa tcgcaggtgg cctttagcct tttccaggtc
     3781 ctttgcaagc gagtcgtatt cgccagaatt cttatctgca gataagatca ttttttcaag
     3841 ctcttctatg gcaagttgat ttgctgagat cagtgacaat ctttcatccg ggtttattac
     3901 agcgataatc ttatcctttt ctgtcctttg attctcacgg taattaagct gctcaattat
     3961 accattcgca ggtgacttaa catcagtaga tatgtttgcc tctatagttc cagttacatc
     4021 aatatatgag atcattgttg tggatgaggc tgattgtacc cttaccagtg gtggagcttt
     4081 tttagctgca gattgcacgg tgttttctcc tttttttctg tcacatgatg ttaatgccag
     4141 aagaactgaa agtgaaacaa gtattgtgat tttaatctta ttcattatct gttgatttaa
     4201 taaggattat tatcgtttcc tgtaagatac ctaagctttg aaaaggcctg aagaaaggca
     4261 atcgttgact tctgataatt aattgtggcc tctgacagga tagtctgtgc atcaagcaca
     4321 tcaataattg taccctgtcc tccctgatat aacactgaag cattttcgag agtctcccgc
     4381 gccagatcta ttatcttctt attatttgag atttcttcct ttatgtcttt aatttcattg
     4441 agagtgatgt ctatttccct gagcagatca acaaaaagct gagccttctc gtcatttatc
     4501 tgttcaaccc tgagatcgct ctgaagcatc tttgttttgt aaccagaacc tccgaaatat
     4561 ggtatggtat aacgtatgcc taccccgata ttatagttga aattttttcc aaaggggata
     4621 taaccgtgtt cccatgtacc attaccgtac gagtataact ccggacggtt tagcaaccgt
     4681 gcaatctcct tctgcttatc ttcaagagtt atcttaagat tggctgcctt tattgacgga
     4741 tgattatcaa gagtttgttt ataaagtgaa tcggggataa gaattcgtga cagatttgaa
     4801 gtagaaatag tatcaatatg cccttcaata agcaaaggat gcggcctctc cgggaagcat
     4861 agcctgccca gttttatctg atgagccaga actgaattca cggatttctg aagtgctttt
     4921 tcttcgacgg agatctggac attgatttta agaacatcca cacccgatac tttacctatt
     4981 ttatacaaag cttcagcgta ccttaaatga gttctgagct ggtaaagagt attatggtga
     5041 acagtaacct cagattcagc tttcaaaatt tcatagtaag tatcttccac tccccatata
     5101 attgtacttc gaagctgccg ctttatttct tcattcaata aaatttcgtc ctttatagcc
     5161 gacttcctga ctttattttc accccactca taaattgtct gataaactga gatatctgta
     5221 taaacatcag taagactatt gccgagaagt cgctgcttgt tcggaaggag gaatttccac
     5281 acggaggctg ttgcagatgc agaaatcgtt ggttgatagg taagtcttaa caactcaata
     5341 tctgactgtt ttacagcaga ctgcttttca tttgccttta tgaggtaatt attttgcaga
     5401 gcgctgtcaa tcagttgttt gagattatag acatatcccg gatcctgtcc tgctgcagaa
     5461 taaatgcata tcaacagcaa aatgtaaatc aggactgctt ttgttttcat tgtcagttat
     5521 ttttcaatat ccatcggtat tagttggcaa aaaccaagga ttattaattg tttattttac
     5581 agatctttga cgttattata atatcgcttc gtaagtatct cttttatagg aatttattct
     5641 agcaacagta agcgataacc acaattattc tggattttca caacattgag attagtgtcc
     5701 gggaaggatt ttatcttaag atgctgcttt atccatttga aagactgaga aaaatttcag
     5761 ttttaaaact cactatgaac aggaacagga ccctgaacaa ctgctgcagg ctagcggcag
     5821 gatactttca aaaggcagcc ctttagcttt ccacgttttc aagccgccat cgagattaac
     5881 agaatttacg aatccgttcc ggttaagcat atttgcaact ttagtgctac gaacaccacc
     5941 ctgacaaaat actataattg gtttgtcatc aggaatattc ttcagattat ccattattgc
     6001 cgacatggga aaattaaaaa catcattaag cgagataaat tcaatcatga actcagattc
     6061 ttcccgcaca tcgacgaata ttgctaaacc tttatctaat tcttccagcg cattttcagg
     6121 tgagatatgc tcaacaccaa atatgttgaa tgactgaaat gaaggtgttt ttggagtgat
     6181 catttttatt ttactccttt ctgctgaagt acttttacaa gctcttcttc gggaaaatat
     6241 ccgacatgcc tgaaatattc actgccgttt tcatcgagaa atacctgagt tggtatagcc
     6301 tgaattctat acttttgagc gtagggcgct ccggcttcgg tccacacatc atagaataca
     6361 acctttactt gatcgccgta cttttcctga attgatttca ttatcggttg catctgctga
     6421 cagggaatgc accggacgga acccaattcg ataaaagtca ctttatacgt ctttttttca
     6481 gaccctgtgt tatttttatc agcttttttt gtctggcctg aacaatcagt gctcagaata
     6541 attgttgcca gggctgcaaa aagcagcaaa gattttctga ttttttccat tatgcaattt
     6601 ttttaatgta ttcaacaatt tcgtttattt tttcatctgt aacaggtgtt tttcccttaa
     6661 ttattccaaa gtcggttgtg ttgacatgaa tgatattggt aatggtaata ttttctttac
     6721 caagtatttt catggcacaa ttaattgcgc atccatccag cactactatt tttttatcct
     6781 gctgtgtctt taatttattt atcagagact ggtcacctac agcaaccttg gcaaggcaaa
     6841 ccatgtttgc ttcaccactc tcgtcaagtt tacgcgcaac tttatcggcg aattcaccgg
     6901 tgtttgacgc gccgctgcaa ctgacgattt gaattttttg tgagttttcc atataggtct
     6961 tatttatttg ccttttcaat taatttttca gcttgatcat agatatcttt tataggccac
     7021 tgctttatca tctttcccgg tccgattgct tctttagcct gttcaaaggt acagcagact
     7081 tccagtgctt tttaaaaatg gtaatcgaga gagaggcggc
//
LOCUS       DAOSNB010000113         7105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10593_length_7105_cov_4.347376, whole genome shotgun sequence.
ACCESSION   DAOSNB010000113 DAOSNB010000000
VERSION     DAOSNB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_7105_cov_4.347376"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1208)
                     /locus_tag="PLG42_05870"
     CDS             complement(120..1208)
                     /locus_tag="PLG42_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU30769.1"
                     /translation="MKKFSFLILISMAFFFRATGQPGTTELMNAINRINSNFERLSHQ
                     FDILEKKTDDIYWYNKVGDIAFIDKVYMTGPPLAVEKNPTGQGAGNPVKFWSYIFIPK
                     DADPSRKYPLLVFPHGGVHSNFDTYYAHIIREMVSQGYIVVAAEYRGSTGYGESFYNM
                     IDYGGLEVQDVDASRQYMIDNYSIVDGDRVGIIGWSHGGYIALFNIFEYPDKYKVCFA
                     GVPVSDLIARMGYKDQSYRDLFSSQPAIGKPADQNVAEYRKRSPAWQVHKYRNTPLLI
                     HTNTNDEDVNVLEVEHLIKSLKAEGKQNFYYEIFNNMPGGHSFDRMDYKEAKEIRMKI
                     YNHLNKYLNPPKPFKSVKDLQKAGYGFN"
     gene            1616..3076
                     /locus_tag="PLG42_05875"
     CDS             1616..3076
                     /locus_tag="PLG42_05875"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOU30770.1"
                     /translation="MKVKTILGGLTMSILGAAIALLVYTNFVEKNSGKGSVVDTSNIS
                     DERVKPVLTSYQQAGQVDLTYAAELAVHAVVHVRTSATYSVRSINPIIEWFYGDSYRE
                     VPREVRGYGSGVIISPDGYIITNNHVIENAEKITVKLNDNREFEAQVVGRDPSTDIAL
                     LKINGENLPYLNYGDSDKLRLGEWVLAVGNPFNLTSTVTAGIVSAKGRNLGILDDNYR
                     IESFIQTDAALNVGNSGGALVNTDGQLVGITTAIISPSGAYAGNSFAIPVNIVKKVVE
                     DLRIYGAVQRAVMGVSVGEVTAEIAEKAKMKEIKGAYISQVYENSAAEEAGIKEGDII
                     IKFNNVPVNSMSELLEQVGRYRPGDKVSVTYIRSGKENTVSLTLKNIENTTGIVKVDA
                     SAGTVFGARLEPLSQADQRNYGVDYGVKVTEVNEGRFRDLGIRKGYIILSVNGKKVKS
                     AADVRNFTDNGTNLTSIEGIQSNGTYFSYQFGGRNR"
     gene            3196..4056
                     /locus_tag="PLG42_05880"
     CDS             3196..4056
                     /locus_tag="PLG42_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU30771.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KGDRAALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQEYERTPSPEELAE
                     VLDLPKEKVSDTLRVSGRHVSVDAPFVEGEDNSLLDVLPNTDSPNADKALIDESLSKE
                     IERALATLTERERDIIKYFFGISCQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKSYLG"
     gene            4187..6202
                     /gene="uvrB"
                     /locus_tag="PLG42_05885"
     CDS             4187..6202
                     /gene="uvrB"
                     /locus_tag="PLG42_05885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOU30772.1"
                     /translation="MNFKLVSDFQPTGDQPEAIRQLVDGLRKGVPYQTLLGVTGSGKT
                     FTIANVIEQVQRPVLVLSHNKTLAAQLYGEFKQFFPNNAVEYFVSYYDYYQPEAYIPA
                     TDTYIEKDLSINEEIEKLRLSTTSSLLSGRRDVIVVSSVSCLYGIGNPEDFYSSTVHI
                     KTGQMISRNHFLRKLVDSLYSRNEIEFRRGTFRVRGDTVDIFPAYADIAIRVQFFGDE
                     VEEISSFDPVEGTKIEVLSEYIVYPANIFVTTRDRINRAIMQIEEDLYKQVAHFRDTG
                     RTEEAKRLEQRVLYDLEMIKELGYCSGIENYSRYFDGREPGTRPFCLLDYFPDDFITI
                     IDESHATIPQIRAMYGGDHARKQTLVEYGFRLPAALDNRPLTLDEFESLTGQTIYVSA
                     TPAEYELRKSEGVFVDQVIRPTGLLDPPVEVRPSLNQIDDLIKEIRSRSKAGERILVT
                     TLTKRMAEELTAYLLKYDIRCRYIHSDIDTLERVEIMEGLRAGEFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNINGKVIMYADTITESMQATIDET
                     NRRRAKQMKYNMEMGIEPAQIVKKKGSILAGLSKQGIRVKAYVEPETPDIAADPVIKY
                     MSREALEKAIEKAKKNMEKAASELNFIEAARYRDEMNELKKLLSQKK"
     gene            6269..6964
                     /locus_tag="PLG42_05890"
     CDS             6269..6964
                     /locus_tag="PLG42_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU30773.1"
                     /translation="MSTDILSLRLGITNTYLLRGSKGIVMVDSGPPSKIRAFKKKLSK
                     LYILPGDIKLLLLTHAHFDHAGLSNEIQNFTDARVMIHRYEWNALEKGQYFDRTGIDL
                     WGKISGIFLSFFARKIFFPLPRPDLIVGDEDISLLDYGVDGTVIHTPGHTPGSLSVVL
                     KTGEAFVGSMAHAGFPYRTKPGFPVFATDLGELKKSWKKLIDYGARIIFPAHGNPFPI
                     ELLKQKIENSSDV"
BASE COUNT         2181 a         1411 c         1596 g         1917 t
ORIGIN      
        1 agctctgtgc tctctcgcta tgcagggctc cgcttcgctc cgtgagccat ttatcctgtg
       61 ccctgagctc tgagctctgt gctcaccagc tacgcggggc tccgcttcgc tccgtgagct
      121 taattaaatc catatcctgc cttctgcagg tctttaaccg acttaaatgg tttcggcggg
      181 ttaagatatt tattaagatg attataaatc ttcattctta tttcctttgc ttccttataa
      241 tccattctgt cgaaagaatg tccacctggc atattgttga agatttcata atagaaattt
      301 tgtttgcctt ctgctttcag ggatttaata agatgttcaa cttccagcac attaacatct
      361 tcgtcgttgg tgttggtatg aatcagtaga ggtgtgttcc tgtatttatg gacctgccat
      421 gcaggcgacc tttttcggta ttcagccaca ttctggtcgg caggtttgcc tatagcaggc
      481 tggcttgaaa acagatcccg gtacgactgg tctttataac ccattcttgc gatgaggtcg
      541 ctcacaggca caccggcaaa gcatacttta tatttgtcgg ggtactcgaa aatattaaac
      601 agggcaatat aaccgccatg gctccatcct atgatgccaa ccctgtcgcc atctacaatt
      661 gaataattat caatcatata ctgccggctg gcatcaacat cctgaacctc aagtccacca
      721 tagtcaatca tattataaaa tgattctcca tagcccgtag agcctctgta ttctgcagcc
      781 accacaatat atccctgtga gaccatttcg cggatgatat gggcatagta agtgtcaaag
      841 tttgaatgaa caccgccgtg agggaacaca agtaagggat actttctgga ggggtcagca
      901 tctttgggta tgaaaatata cgaccagaat tttacaggat ttccggcgcc ctgtccggtc
      961 ggatttttct cgacagccag agggggtcca gtcatgtaaa ccttgtcgat gaaggcaata
     1021 tcgccaactt tgttgtacca gtagatgtcg tcagtctttt tctcaagaat gtcgaactga
     1081 tgcgataagc gttcaaaatt tgaatttatt ctgttgatag cattcatcag ttctgttgtt
     1141 ccgggttgtc ccgttgctct gaagaaaaag gccatagaga tgaggattag aaaagaaaac
     1201 tttttcatgg tttgtgattt tggattttcg tttcagaatg ttgtcatttt aaaaaaaaaa
     1261 attttctgct caaatttaat cttattgaac caattatttg gtctatgtca atatatcatc
     1321 atggctgcca tattgacagt ctgtaataat ataggtaaat tgagaaataa atgttggttt
     1381 aagggattaa attttacctg ttgtttgtct ctttgttgat gcaggtcgaa agcaattatt
     1441 gatttgttaa atgacattat caatcacgtt aaattgatgt taaatgtgat tgaaactgag
     1501 ttccatgaat aaaaaatgca taatctttgt taaacaattt aactggtttg aggtttaagt
     1561 aaggaatcct cttaccagaa taaaaatata tgtctaacca aaaaataaag cagttatgaa
     1621 agtcaagaca attctcggcg ggttaacgat gtcaattctt ggggcagcga ttgctctgtt
     1681 agtgtataca aattttgttg agaagaattc aggaaagggt tcggtagttg acacttcaaa
     1741 tatatcggat gagagggtaa aacctgttct gacttcttat cagcaggcag ggcaggtcga
     1801 tttaacctat gctgcagaat tagcagttca tgctgtggtg catgttagaa caagcgccac
     1861 ttattcagta cgcagtataa atccgataat tgagtggttt tatggtgatt cttatcggga
     1921 ggttcctcgt gaagttcgcg ggtacgggtc gggtgtaata atctctcccg atggttatat
     1981 tatcacaaac aatcacgtaa ttgagaatgc tgagaagatc acagtcaaac ttaacgacaa
     2041 cagagagttt gaggctcagg tcgttggccg tgatcccagc actgatattg cgctactgaa
     2101 gattaacggg gagaacctgc cttatctgaa ctatggtgat tctgacaagc ttcgccttgg
     2161 tgaatgggtt cttgccgtag gaaatccttt taatcttaca tctacagtta ctgcaggtat
     2221 tgttagtgcc aagggaagaa atctgggtat tctcgacgac aactatcgta tcgaatcatt
     2281 tattcagacc gatgctgcac ttaatgtagg taacagtggt ggtgcacttg taaataccga
     2341 cggacagctt gttggtatta caactgcaat aatttcacca agtggtgcat atgcaggaaa
     2401 ctcatttgca attcctgtta atatagtaaa gaaggtagtt gaggacttga gaatctacgg
     2461 tgctgtacag agagcagtga tgggtgtcag tgtgggtgag gtcactgcag aaattgccga
     2521 aaaagcaaaa atgaaagaaa taaaaggagc atatatcagc caggtgtatg aaaacagcgc
     2581 tgcagaagaa gctggaatca aagaaggtga tatcattata aagttcaaca atgtcccggt
     2641 gaacagcatg tcggagctgc ttgaacaggt tggcagatat cgtccgggtg acaaagtctc
     2701 ggtaacttat attcgttccg gaaaagaaaa tacagtttca ttaacactta agaatattga
     2761 aaataccact ggaatagtaa aagtagatgc cagcgcagga acggtatttg gtgcaaggct
     2821 ggagcccctt tcacaggcag accagagaaa ttatggagtt gattatggag ttaaggtaac
     2881 tgaggttaat gaaggcagat tcagagatct tgggatacgc aaaggctaca tcattctgag
     2941 tgtaaacgga aagaaggtca aaagtgctgc tgatgtcaga aactttaccg acaacggtac
     3001 gaatttgact tcaattgagg gcatacagtc gaacggaact tacttcagct accagtttgg
     3061 aggtagaaat agataatata aattgttaaa gaacagggag ttaataaatt taaactccct
     3121 gttcacaata attgttaaaa ttctttgttt ttaaatatat ttttattaat ttcgcgaaaa
     3181 ttttttcaga gatagatgag acaacttaag attaccaaat caatcaccaa cagggaaagc
     3241 gcttcactcg acaagtacct tcaggagatt ggtaaggaag agttgatatc ggttgaggag
     3301 gaagttgaac ttgcacagag aatcaaaaag ggagacaggg ccgcactcga aaaactgact
     3361 aaagcaaatc ttcgatttgt ggtatctgtt gcaaaacagt accagaatca gggactgagc
     3421 ctgcctgatc ttatcaacga aggaaacctg gggcttatta aagccgcaga aaagtttgat
     3481 gagacaaggg gattcaaatt catctcctat gctgtatggt ggatcaggca atcaatactt
     3541 caggccctcg ctgaacagtc aagaatagtg cgtctgcctc ttaaccaggt tggttctctg
     3601 aataagatca ataaagcctt ctcgaaattc gaacaggaat acgagagaac accatctccg
     3661 gaagaacttg cagaagtgct tgatctcccc aaggaaaagg tttccgacac actccgcgta
     3721 tcaggcagac atgtgtcggt tgatgctcct tttgtcgagg gtgaagataa tagccttctc
     3781 gatgtgctgc ctaacaccga ttcccccaat gccgataaag ctcttattga tgaatctctt
     3841 tcaaaagaga ttgaaagagc cctggcaaca cttaccgaaa gagaaagaga tatcataaaa
     3901 tactttttcg gtatttcatg ccaggaaatg acacttgagg aaatcggaga gaaattcggg
     3961 cttacacgcg aacgcgtcag acagataaag gagaaggcaa taagaagact gagacacact
     4021 tccagaagta aactgctgaa gagctattta ggctaggcta aaaactgttt aacaaagccg
     4081 ccggaaaaac ccggcggctt tgttgtttct gaaatgctat cattgttttt tgtaaatttc
     4141 cgcttatatt caattctatt acggccgact gaaagttgtt agtgtgatga attttaaact
     4201 tgtatcagat tttcagccga caggcgacca gcctgaagca atccgccagc ttgttgacgg
     4261 tctccgtaaa ggtgtgccat accagactct ccttggcgtg acaggctcgg gcaagacttt
     4321 tactatagcc aatgtaatcg aacaggttca gagaccagta cttgtcctca gccataataa
     4381 aacactcgct gcacagctgt acggagaatt caaacagttc ttccccaata atgctgtgga
     4441 atattttgtc tcgtactacg attattacca gcccgaagca tatatccccg caactgacac
     4501 ttacattgaa aaagaccttt caattaatga agaaatagaa aaactccgcc ttagtacaac
     4561 ttcctcactt ctttcaggca gacgcgacgt aatagtggtt tcatcagtat cttgtctgta
     4621 tggcatcgga aaccccgaag atttctattc aagcacggta catattaaaa ctggtcagat
     4681 gatttcgaga aaccacttcc tgagaaaact ggtcgatagt ctctattcaa gaaatgaaat
     4741 cgaattccgc aggggaacct tcagagtaag aggtgatacg gtagacatct ttccagcata
     4801 tgccgatata gcaataagag tgcagttttt tggtgatgag gtagaagaaa taagctcatt
     4861 tgaccctgtt gaaggaacaa aaatagaggt actgagcgaa tatatagtct atcctgcgaa
     4921 tatcttcgtc acaacgcgcg acaggataaa cagggcaatt atgcagatag aggaagatct
     4981 ctataaacag gttgctcact tcagggacac cggcaggaca gaagaggcta agaggcttga
     5041 acagagggtg ttgtatgacc ttgaaatgat aaaggaactc ggttactgca gcggcataga
     5101 aaactattca aggtattttg acggcaggga gcccggcacc cgccctttct gcctgcttga
     5161 ttatttcccc gatgatttta taacaataat agacgaaagt catgctacca tacctcagat
     5221 aagagccatg tacggaggtg accacgcccg caaacaaacc cttgttgaat atggctttcg
     5281 cctgcccgcc gcactcgata accggcctct taccctcgat gagtttgagt cactaaccgg
     5341 acagactatc tacgtgtcgg caacaccggc cgaatatgaa ctgcgtaaaa gcgaaggtgt
     5401 ttttgtcgac caggtaatcc ggccaaccgg actgcttgac ccgccggtag aggtgagacc
     5461 aagcctgaac cagattgacg acctgataaa agaaatacgc agcaggtcaa aagcaggtga
     5521 gagaatactt gtgacaacac ttacaaaaag aatggcagaa gagcttactg cctatctttt
     5581 gaaatatgat atcaggtgcc gttatatcca ttctgatata gatacccttg agagagtgga
     5641 gattatggaa gggttacggg caggggagtt tgatgtgctg gtgggagtga accttctgcg
     5701 cgaaggactc gaccttcccg aggtttcact ggtggcaata ctcgatgccg ataaagaagg
     5761 attcctgcgt tcggcaagat cactcaccca gacagcagga agagcagcaa gaaacattaa
     5821 cgggaaggtg ataatgtatg ccgacactat caccgaaagc atgcaggcta ccattgatga
     5881 aaccaacaga agaagggcaa aacagatgaa atacaacatg gaaatgggta tcgaacctgc
     5941 acagattgta aagaagaaag gttcaatact tgccgggctt agtaaacagg gcatcagggt
     6001 taaagcttat gtggaacccg aaacacccga tatcgcagcc gatccggtaa taaaatatat
     6061 gagcagggaa gcactcgaaa aagcaataga aaaagctaag aagaatatgg aaaaagcagc
     6121 ctcggaactg aacttcattg aggcagccag ataccgggat gaaatgaacg aactgaaaaa
     6181 acttctttca cagaaaaaat aacctgccgg tttgcaatta ttccagaaca gttcgtaaat
     6241 ttattgagaa cctaaaatca tttctcctat gtcaaccgat attctatcgt taagactcgg
     6301 aatcacaaat acatacctgt tacgtggatc aaagggtata gtgatggtgg actcaggtcc
     6361 gcccagtaaa ataagagctt tcaaaaaaaa gctgtcgaaa ctctatattc ttccgggcga
     6421 cataaaactg ttacttctta cccatgctca tttcgatcat gccggacttt caaacgagat
     6481 acagaatttc acagatgcca gggtaatgat ccacaggtat gaatggaatg cacttgaaaa
     6541 aggacaatat ttcgacagaa caggtattga cctctggggt aaaataagcg gaatttttct
     6601 ttctttcttt gcccggaaaa tctttttccc gctgcctcgt cccgatttaa ttgtaggtga
     6661 tgaagatatt tccctgctcg attatggggt tgatggcaca gtaatacata cacccggtca
     6721 taccccggga tcgctgagcg tggtgctgaa aacaggtgaa gcttttgttg ggtcgatggc
     6781 tcatgcggga ttcccttaca gaacaaaacc cggatttccg gtgtttgcta cggatctggg
     6841 agaactgaaa aaaagctgga aaaaacttat tgattatggt gccagaatta tctttcctgc
     6901 tcatggaaat ccatttccga ttgagcttct gaaacagaaa atagaaaact cttctgacgt
     6961 ctgagaaact ggcacacagt cagttccctg taaaaaattc cgtcataaat gaacggttat
     7021 tttccggcat caattctttt tcctgcaaga aacagtacta ctgatatcag aagaaaactg
     7081 tttgctgcat gaaaataatt tacca
//
LOCUS       DAOSNB010000114         7092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10628_length_7092_cov_4.245559, whole genome shotgun sequence.
ACCESSION   DAOSNB010000114 DAOSNB010000000
VERSION     DAOSNB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10628_length_7092_cov_4.245559"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLG42_05895"
     CDS             complement(<1..721)
                     /locus_tag="PLG42_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU30774.1"
                     /translation="MKRLFIYFIVILLTISCASQQQLADYGLPRSSAFNFIIISDYGR
                     NGFAHQKEVAEIMGNIAEKVNARFIVTGGDNFQISGVENVSDPLWYSCFENVYTHPSL
                     HVEWYPAFGNHDHLGSLQAQIDYSKISRRWKMPAPYYTLVKTRDSVSLRLVILDTQSL
                     VNGLGNPSKRYTTEDAMKQIHWADSVLASEKEDWVIVVGHHPVYSAHPTRGNTEELVE
                     YLRPVLNKYNVDFYIGSHDHIF"
     gene            1110..3011
                     /locus_tag="PLG42_05900"
     CDS             1110..3011
                     /locus_tag="PLG42_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOU30775.1"
                     /translation="MKASLLEEILQSIKNVRIAVVGDFCLDAYWFVDESKSEISVETG
                     LPTRPVRKQAYSPGGAGNVANNLAAMEVGEVLAFGVIGPDPFGVEMVKILNEKGINTS
                     NLLIQEKEWATHVYIKPYSGETEANRIDFGNFNRLSRKTADKLISRLTGAMQKIDLVI
                     LNQQVLSGINTEYFRMRLTGVIKEFPDKIFIADSRKYSDSYHGAYRKMNDTEAFRLCG
                     VTKTADEEIPVSEVKKVARELYGRYEKPVFVTCGKKGSVVADEKNTYEIPGLMILSKI
                     DTVGAGDSYLAGVASALAAGYSVDTAAELGTLVAGVTIQKLFQTGTATPEEILNIGRD
                     PDFIYNPDLAENPHMARYLGKTEIEIINHLSDKAEIRYAIFDNDGTISTLREGWENIM
                     APVMEKAILGDRYEDADEAILRKVKKRVKEFIDQTTGVQTLVQMKMLPSIVREFGMVP
                     EEKILNEFEYKKIYNDELMQMVKKREEKLKAGELSVEDFTIKNSINFLQMLYDRGIKL
                     YLTSGTDVEDVVHEAEVLGYAHFFEGRIFGAVGDVEKDAKKIVLDRILDMIGEKDSRM
                     IVTFGDGPVEIRETHKRGGITIGVASNEQRRHGLNLTKRTRLVKAGADIIIPDFSQAE
                     HLFQLLNIR"
     gene            3013..4143
                     /locus_tag="PLG42_05905"
     CDS             3013..4143
                     /locus_tag="PLG42_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30776.1"
                     /translation="MEFPKFDRKKLYIRNLSERVNKLSVEKILILPGTKPSIENIETI
                     RLIEESAERIIDARKNNKPVVMAFGAHVIKNGLALVINEMIKKGWITHLAGNGAVSIH
                     DWEFSFAGETSEDVRENVRNGCFGIWEETGKYINLALLTGAYYGYGYGEAVGKMILHE
                     GLEIPGPQLLEQEACNLLYDNCGKAAAAADLLGIIKSEQLQTGFMHIPHRWKKYSVLC
                     AAAELNIPFTIHPMFGHDIIYTHPLNHGAAIGRTALTDFLRFVHSISNIDGGVYLSVG
                     SAIMSPMIFEKALSMAQNIAIQENRHIDNHFIAVVDLAKSNWDWLRNGEPPSDNPAYY
                     MRYNKTFSRMGGTMRYITADNRDFLTGLYNCLSEHNNHSLLA"
     gene            complement(4135..5748)
                     /locus_tag="PLG42_05910"
     CDS             complement(4135..5748)
                     /locus_tag="PLG42_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOU30777.1"
                     /translation="MNLVLTNLDWIILSFVIISTLSYGLYMAFAKKAGQSSSNFFLGG
                     RSIKWPIVGASLFATNIGAEHLVGLSGDAYRYGLAAGSVELTTAITLGFTCAFLLPCY
                     MRNKVFTIPEFLELRYDRRARIFFSGLMLLISIMTKLAFTLFAGALVLNGILGWDIME
                     TIFYIGIIVALFTILGGFTAVAYTDSLQVIIMILGSAIMLFIGLHKTGGWSGLVEKAP
                     QMISIAKPYDDPVYPFWGIIATAFYAGIFYWGIDQVNVQRLLAAPDLKQARWGAMFAT
                     FLKLFPVFLFALPGVIAFALYPGELTGDATRQTFVLLLNKLMPEGLRGLLIASLLAAL
                     ITSLIGVLNSVSTLIVRDFIVEFHPATSERRQVTYGRLSILLVTLLGIGAAYLVYKNE
                     EGIYKYLQTISAYLVIPVFPAIFFGVVSKRVNMKGAIASVIAGIILATVFVIDQFLGY
                     DTGKTVFPFLHHKLTLNFGYRGLWAEMLITVILFGVSAFTGRTDAEKLEKATINLKKG
                     AGKFEGLKDWRLQLAVLSVVTIILYVWLS"
     gene            complement(5799..7070)
                     /locus_tag="PLG42_05915"
     CDS             complement(5799..7070)
                     /locus_tag="PLG42_05915"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOU30778.1"
                     /translation="MKREEIVSLKAEVENHLKNELLPFWTSRMIDRVNGGYITHFDRH
                     GRDTGEDEKSLISQTRSLYTLSSALRSGFVEGDYKGMACHGVEFLLEKMWDREYGGFY
                     WMADRRGNIKIDKKIIYGHSFAIYSLSEYSLATGDSRGCDYACRVFDLIQKYCADTFY
                     GGYWEMFNRNWTLCGPGSQGGDRKTLDVHMHLMEAFTTLYECTGEEVHRRKLIEVINL
                     LLKRIMHPVYRTGIPQFFGDWSIAPQIKFDIIWGWDRFTGEGVKPNVTDNTCYGHNAE
                     FAWLLIHALEILKEPVENYKDDLRKIFDHTVDNGIDWEYGGVFVEGPHAGGVSDREKE
                     FWQQAEVLIGLLDAYMIFKDEKFWEAYKNVHRFVFDKMINREVGEWFPLMTREGEPIW
                     THMGHSWKINYHTVRAMIQCLGKLNQLLEKV"
BASE COUNT         2163 a         1584 c         1447 g         1898 t
ORIGIN      
        1 ggaatatgtg gtcgtgtgaa ccaatataga aatcaacatt atatttattt aataccggcc
       61 ggaggtattc cacaagctct tcagtgttac cccttgtcgg atgcgcgctg taaacaggat
      121 gatgtccaac aactatcacc cagtcttctt tctccgaggc aagcacactg tcggcccagt
      181 gaatctgttt catggcatcc tcggttgtgt atcttttaga tggattgcca agtccattaa
      241 ccagcgactg ggtgtcaagg attacaagtc gcagactcac actatccctt gttttaacta
      301 aagtataata cggagcaggc atcttccagc ggcggctaat ctttgaataa tcaatctgtg
      361 cctgaagact gcctaaatga tcatgattgc caaaagccgg ataccattca acatgaagcg
      421 acgggtgtgt atatacattt tcaaaacagg aataccataa aggatctgaa acattttcta
      481 ccccacttat ctggaaattg tctcccccgg tcacaataaa ccttgcgttc accttttctg
      541 ctatgtttcc cataatttct gccacctctt tctgatgagc aaaaccatta cggccatagt
      601 cacttataat aataaaatta aaagcggaac ttcggggaag accataatct gctaactgct
      661 gctgagaggc acaggatata gtcagcagaa taacaataaa ataaataaat aatcttttca
      721 ttctatcccg tttattgtca aagttaaata tttaagccat tttacaaata aaacatttat
      781 tttttgaaaa atgcaatttt ctttccttat cataaatgtg ttccgtactg aaaaaatatt
      841 ttctcaccca tcaacggggc tgtctaaaaa gccccttttg ctttcacccc tgaatccaag
      901 cctttactac tgtaaagccg ggctgctatg aggaaaaact tattgatata caatatttta
      961 gctttcgtgt tttcattata cttacctgat acacatcttt ttagaaaacg ccagaggtat
     1021 cttcctgtaa acatgtttca ggctatgaca ataatgatgt ttcgaggtat atttttattt
     1081 ttataaaaca tttcaaaaat aaagtttata tgaaagccag ccttcttgaa gagattctcc
     1141 aatcaataaa gaatgtgcgt attgctgttg ttggtgattt ttgcctcgat gcctactggt
     1201 ttgttgatga atcgaagagt gaaatctcag ttgaaacagg tcttccgacc cgtccagtca
     1261 gaaaacaggc atattcgccc ggtggtgcag gaaatgtggc aaacaacctt gctgccatgg
     1321 aagtgggtga agttctcgct tttggagtca taggaccaga tcctttcggc gtagagatgg
     1381 ttaagatatt aaatgaaaaa ggcatcaata cttccaatct tttgattcag gaaaaagagt
     1441 gggcgaccca tgtatatatc aagccctatt cgggagaaac agaagcaaat cgcattgatt
     1501 ttggaaattt caacaggctt tcacgaaaaa cagccgacaa acttatctcg cgacttaccg
     1561 gggctatgca gaagattgat cttgttatcc taaatcagca ggttctctcg gggatcaaca
     1621 cggaatattt cagaatgagg cttaccggag tgataaaaga gtttccggat aaaatcttca
     1681 ttgccgacag caggaaatat agcgactctt atcatggtgc ataccgtaaa atgaatgaca
     1741 ctgaagcttt cagactttgc ggcgtaacga agactgccga tgaagaaata cctgtttccg
     1801 aagtaaagaa agttgcaaga gagttatatg gcaggtatga aaaacccgtc tttgttacat
     1861 gcgggaagaa aggctcggta gtagccgatg agaagaatac ctacgaaata cccggtctta
     1921 tgattctttc aaagattgat actgtaggtg ctggcgacag ttaccttgca ggtgttgcct
     1981 cagccctcgc tgcaggatac tctgttgaca cagctgccga gcttggtaca ctggtagcag
     2041 gcgtgaccat tcagaaactt ttccagacag gcactgcaac acctgaagag attcttaata
     2101 ttggcagaga ccctgatttc atttataacc ccgacctggc agaaaatccg catatggcca
     2161 gatacctcgg taaaacagaa attgaaatca taaaccacct ctcagataaa gctgagatca
     2221 gatatgccat tttcgacaat gatggaacca tctccacact gagggaaggc tgggaaaaca
     2281 tcatggcacc cgtgatggaa aaagccatcc ttggcgacag gtatgaagat gctgatgaag
     2341 caatcctgcg aaaagtaaaa aagcgtgtaa aggaattcat cgatcagaca acaggtgtac
     2401 agacccttgt acagatgaaa atgctcccat caatagtaag agaattcgga atggttccgg
     2461 aggagaaaat acttaacgag ttcgaataca aaaagattta caacgatgag ttgatgcaaa
     2521 tggtaaaaaa acgtgaagaa aagctgaaag ccggtgaact ttcagttgaa gattttacaa
     2581 tcaaaaacag cataaatttt ctgcagatgc tttacgaccg tggcataaaa ctttacctca
     2641 caagcgggac cgatgttgaa gatgttgttc acgaagctga agtacttggg tatgcccact
     2701 tttttgaagg ccgtatcttc ggtgccgtgg gtgatgtgga aaaagatgcc aaaaagatag
     2761 tactcgacag gatacttgac atgataggtg agaaagactc aagaatgata gtcactttcg
     2821 gtgacggacc tgttgaaata agggaaacac ataaacgagg cgggataacc ataggcgtag
     2881 ccagtaacga acagaggcgt catggactaa acttaacaaa acgcacacga ttggtcaagg
     2941 ccggcgccga cataattatt ccagactttt cccaggctga acacctgttt caattattaa
     3001 atatcaggta aaatggaatt tccgaagttt gatcgcaaaa aactgtatat aagaaattta
     3061 tcggaaaggg taaataaact ctctgtagaa aagattctta ttttgcccgg cacaaagcct
     3121 tctatcgaaa atattgaaac aataaggctt atcgaagaga gtgccgaaag gatcattgat
     3181 gcccgtaaaa acaataagcc tgttgtgatg gcatttggag cgcatgttat taaaaacggg
     3241 ctggcgttgg taatcaacga aatgattaaa aaaggatgga ttacccacct tgccggtaac
     3301 ggggcggtaa gcatccacga ctgggaattt tcttttgccg gtgaaacaag cgaagatgtg
     3361 cgtgaaaatg tcagaaacgg ctgcttcgga atatgggagg agaccggtaa atacatcaat
     3421 ctggccctgc ttacaggggc atattacggg tatggttacg gtgaggctgt cggcaaaatg
     3481 attctccatg aagggcttga aattcccggt cctcaacttc tcgaacagga ggcatgtaac
     3541 cttttatacg acaactgcgg taaggcagcg gctgctgccg atcttctggg tatcataaaa
     3601 agtgaacagc tgcagacagg cttcatgcac atacctcacc ggtggaaaaa atattcggtg
     3661 ctgtgtgctg ccgcagaact taatataccc tttaccatcc atccgatgtt cggacacgat
     3721 attatctata ctcaccctct caaccatggc gccgccatcg gcagaacagc attgacagac
     3781 ttcctgagat ttgtacacag catcagtaac atcgacggtg gagtatattt atcagttggc
     3841 tcagccatta tgtcacccat gattttcgag aaagcactct caatggctca gaatatagct
     3901 attcaggaaa accgacatat tgataaccat tttatagccg ttgtggacct tgcaaaatcg
     3961 aactgggact ggctaagaaa tggagagcca ccctcagata atccggcata ctacatgaga
     4021 tacaacaaaa ccttctcccg gatgggcggc acaatgagat atatcactgc cgacaaccga
     4081 gattttctga caggattata caactgcctc agtgagcata acaaccattc acttttagct
     4141 taaccacaca tataaaataa tcgtaacaac tgacaatacc gccagctgga gcctccaatc
     4201 ttttaaacct tcaaatttcc cggctccttt tttcaggttt attgttgcct tttccagttt
     4261 ttcagcatcg gtccttcctg taaaagcaga cacgccaaac aggataactg taattaacat
     4321 ctctgcccag agtcctctgt aaccaaaatt cagagtgagt ttatgatgaa gaaatggaaa
     4381 aaccgttttt ccggtatcat aacccagaaa ctggtcgatg acaaaaactg tagcaagtat
     4441 tatacctgct ataaccgagg ctatagctcc tttcatatta actcttttgc ttaccactcc
     4501 gaaaaagata gcaggaaata caggtattac cagatatgct gaaatagtct gaaggtattt
     4561 ataaattcct tcctcgtttt tataaacaag atatgctgcc ccgatgccaa gaagagtcac
     4621 cagtaatatt gacagacgtc cataagtgac ctggcgtctt tctgaagtgg ccggatgaaa
     4681 ttctacaata aaatctctaa ctatcaacgt tgaaacagag ttcagcacgc cgataagtga
     4741 agttatcagt gctgccagca gggatgcaat gagaaggcct cgcagtcctt cgggcataag
     4801 tttgttcagc agcaggacga atgtctggcg ggttgcatcg ccagtcagtt cacccgggta
     4861 aagagcgaag gctatcacac cgggcagagc aaaaaggaac accggaaaga gcttgaggaa
     4921 tgttgcaaac attgcgcccc atctggcctg tttcaggtcg ggtgctgcca gcagccgctg
     4981 tacattcacc tgatcaatgc cccagtagaa aatgcctgca taaaatgcag tggcaattat
     5041 tccccagaaa ggataaacag gatcatcgta aggttttgct attgatatca tctgtggagc
     5101 cttttcaaca agcccactcc atcctcctgt tttatgcaga cctatgaaaa gcattatggc
     5161 agaacccaga atcattatta ttacctgcag cgaatcggta taggcaactg cagtgaaccc
     5221 acccagtatg gtgaaaagag caacaataat acctatgtaa aatattgtct ccattatatc
     5281 ccagccgaga atcccattca gtacaagcgc tccggcaaag agtgtgaagg caagttttgt
     5341 catgatgctt attaaaagca taagccctga gaagaatatg cgcgcccgcc tgtcgtatct
     5401 caattcaaga aattcaggta tggtaaacac cttgttcctc atgtagcatg gcagcaagaa
     5461 tgcgcaggta aagccaagtg taatagcagt tgtaagttca accgagcctg ctgccagccc
     5521 gtaccggtaa gcatcacccg ataaccccac cagatgttct gctccaatat ttgtagcaaa
     5581 cagcgaagct cctacaatcg gccactttat tgaacgtccg cccagaaaaa agttggaact
     5641 gctctgaccc gctttctttg caaatgccat ataaaggcca tacgacagag tgctaataat
     5701 aacaaatgaa agtatgatcc agtcgagatt cgttaaaaca agattcataa tataactaaa
     5761 aaattagttt caggtgttat caggttagag actgtttttt ataccttttc aagcaattgg
     5821 tttagttttc caagacattg tatcattgct cttactgtgt gataattaat tttccatgag
     5881 tgccccatat gtgtccatat aggttcacct tcacgggtca tgagcggaaa ccactctccc
     5941 acttcacgat tgatcatttt atcaaacacg aaacggtgta cattcttata tgcttcccag
     6001 aatttttcat ctttaaaaat catgtatgca tcaaggagtc cgataagcac ttctgcctgc
     6061 tgccagaact ctttttcgcg gtccgaaacc ccacctgcat gtggaccttc aacaaaaaca
     6121 ccaccgtatt cccagtctat tccgttatca acagtatggt cgaatatctt cctcagatca
     6181 tctttgtaat tttcaacggg ttccttcaga atctcgagag catgaatcag cagccacgca
     6241 aattctgcat tatgtccata gcatgtattg tcagttacgt ttggctttac accttctccg
     6301 gtgaaacggt cccagcccca gatgatatcg aatttaatct gcggagcaat agaccagtct
     6361 ccaaaaaact gcgggatacc tgttctgtat acagggtgca taatcctctt caacagcagg
     6421 ttaatcacct ctatgagttt cctccggtga acttcctcac cggtgcattc gtagagcgtg
     6481 gtgaatgcct ccattagatg catatgcaca tcgagcgttt ttctgtcacc accctgactg
     6541 cccggcccgc acagtgtcca gttgcggttg aacatctccc agtatcctcc ataaaatgta
     6601 tcggcacaat atttctgtat cagatcgaac accctgcagg catagtcaca cccccggctg
     6661 tcgcctgtcg caagtgaata ttcgctgagg ctgtagatag caaaactatg accatagatt
     6721 atctttttgt caattttaat attacccctg cggtcagcca tccaataaaa accaccatat
     6781 tccctgtccc acatcttttc aagcaaaaac tctactccgt gacatgccat ccctttataa
     6841 tcaccctcta caaagccaga cctcagcgca gacgaaagag tgtaaagaga tctcgtttgg
     6901 gatatcagcg atttttcatc ttcgccggta tctctgccgt gcctgtcgaa atgagttata
     6961 tagcctccat taacccggtc aatcatccgt gatgtccaga aaggcaaaag ctcatttttt
     7021 aaatgattct caacctcagc cttcaatgaa actatttctt ctcttttcat atgcaataaa
     7081 ttttataaat tc
//
LOCUS       DAOSNB010000115         7004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10805_length_7004_cov_4.288243, whole genome shotgun sequence.
ACCESSION   DAOSNB010000115 DAOSNB010000000
VERSION     DAOSNB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10805_length_7004_cov_4.288243"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLG42_05920"
     CDS             <1..814
                     /locus_tag="PLG42_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30779.1"
                     /translation="NSVLEIESPFPWKVPDAVCFTDTLSETRMKLASMLSFNYQTSRI
                     YVTKYRKDAREDRGLTTRERKFYGVPDLVVRLDDYIKLPVMYEVFFELVPGVRFRERK
                     SGAEMRVLEPLSNIFYNEPPLAMIDGVIIQNLSLIANLDPEIVEKIELVRSRYMIGDL
                     YFSGIVNVITRKGDLSNIVLPDYAVNIRYRVTENPASFSPPDYSTEEKKESRIPDLRN
                     TLYWNPSAIPGEQGVVTAEFWTHDQAGEYSIVATGVTQSGKLISTIHSFRIR"
     gene            complement(881..3661)
                     /locus_tag="PLG42_05925"
     CDS             complement(881..3661)
                     /locus_tag="PLG42_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30780.1"
                     /translation="MKTSAFISVVIFTVALFSLRLSAQNDRVKIARLSEFVMQSSFVV
                     SETGEQLSDPGFRPGIWWMPVKVPTTVLSALVANKIYPDPYLGLNNMLIPDASDEFNK
                     QYNLEQYSHLPGLPNPWSKPYWFRTEFMVPAEDKGRHFELIFKGINYRAGVWLNGKQI
                     ADSTQMAGMFAEYRLDVSDCIIPGSKNALAVKIYPLDYPGLPDTEQLKALGDFYLNGG
                     KTGDIGKNVTMLCSVGWDWIPPVRDRNIGIWQPVYLRTSGHVTIEQPKLVTLLPNLPD
                     TTLAKISLSFKLKNSGKSTEKGKITVIITPETFQGKPVQFTKDVSLNGNSSLVVAYDA
                     DNTRELLFSKPALWWTNGYGRPDLYRIRIQYSNQSGLSDEISFLFGIRTVGSKAVQVG
                     KMWRRDFYVNGRRIHITGGAWVPDMMLNRDSMRYDYEFRLCRNANINLVRIWGGGVTP
                     PDWFFDAADRYGLLVWSDFWITGDTQGEFKGSPDWPLDGEIFKKNVTSTIYRIRNHPS
                     LLVWTGGNEGHARRELYYAMRDSVIKLDGTRPFIPCSSGFARLPEGWDASWPDGLPGG
                     VYSGGPYTWQDPRDYYRRVDAAADWVFKDETGILSQPPYSTLPKIITNLVWDKSLPFP
                     LNHSWGYHDAATGNGRYDRYYNEMIKRYGTTSTMKEFSDKMQLMNASGYQAIFEAPAH
                     KLNDVGGVMLWKLNAAFPSVVWQVYDWYLMPNAGYYFMQNACEPVHVQFNPKDLKVEY
                     VNRTYKSFSNLKSEIKIFDLKSKLIYSQNETVSLEGPSSAGSLSLSDALSKNTGVSFI
                     ILNLYDNSGKRLSHNAYWYSSSNDFTSLNSMPRTKVNVKLAGVLKGSNELKWSFTLTN
                     SSDKIAFFINPRLMADNEEITPSFWSANYFTLAPGESISVTVGCPPEKISGKKAVLKV
                     EGWNIEEIELKL"
     gene            3762..3950
                     /locus_tag="PLG42_05930"
     CDS             3762..3950
                     /locus_tag="PLG42_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30781.1"
                     /translation="MKRILVDMDGVLADVYSRFFELHEKETGVRLTVEEVAGKLEAEA
                     FPHQIKWVRRLAFSGVYL"
     gene            3911..4273
                     /locus_tag="PLG42_05935"
     CDS             3911..4273
                     /locus_tag="PLG42_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30782.1"
                     /translation="MGTTPGFFRSVPVMEGSVEGLRRLNEKYHVVVISLATEFPSSLT
                     DKQMWLHEHFPFISWRQIVFCGDKSLIKADIMIDDHPKNLDVFQGGTIIFTQPHNMLL
                     ETRHRRVSTWKEIEEILL"
     gene            complement(4641..>7004)
                     /locus_tag="PLG42_05940"
     CDS             complement(4641..>7004)
                     /locus_tag="PLG42_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30783.1"
                     /translation="EANNVITNPYYGTNFNKIPGQISNRNRSIPTDSPFSVAWWYRTE
                     FQLPAAYKGKHIWINFHSINYKANIWLNGKLVADTTTIEGAYRLFTLDITKEALPGGN
                     NCLALEIFPPKSTDLSITWVDWNPTPPDNGTGIWYDVTVHATGDVSIEEPFVKTKLNI
                     PSNDEARLTVSVLLRNSFSKPVSGKLTGKIEKITFSKQVSLNAGETKKVVFEPSEFRQ
                     LIIKNPRLWWPHNVGPQNLYDLNLSFEASGRVSDMRKVRFGIREITSWMNEFDSLKTR
                     VFQVNGKNIVIRGGGYVQDLFLKPSNERIDADIAYAKHMNLNALRMEAPRGSDYMFDR
                     CDEEGILLMVGWCCCSMWERWKTWTPHTADIAELSWRDQIVHLRNHPSVFVWLYGSDN
                     YPPEDVERRYIKVLDEYDGTRPYLSSATQAASKIAGPTGVWMGPYPRVYAYFPPSYWY
                     SKLEFNTEAGPSGEQIPPLETMRMMMPEEDLWPPSESWELRLHKNFYPIARAAFESRY
                     GKPSSVEEYSLKAQIFQNEAVKAMFEAYAANKYKSSGIIYWMYNGAWPKMYWQLYDYF
                     YMPNGAFYAARSACEPLHVLYSYKEHTVKIVNSLYREFKGLKVSARIFDFNSREVWSK
                     TAQADVEPDESKNVFAVEVPANITKIYFLRLEMKDAEGKPVSSNFYWLHADGDEKADF
                     KDLMNLPKTNLKVDATPLQQKGNRARITVTLENTGKNLAFGVNPKILRSSSKGPVLPV
                     FWDDNYFSLLPGEKRVVNVEFDVKELKGEKPLLVIESWNTDLLRLDL"
BASE COUNT         1893 a         1524 c         1497 g         2090 t
ORIGIN      
        1 aaattcagtt ctggaaattg aatcaccgtt tccatggaaa gtcccggatg cagtatgctt
       61 taccgataca ttgtcagaaa caaggatgaa actggcttcg atgttgagct tcaattatca
      121 gacttctaga atttatgtca caaaatacag gaaagatgcc cgggaagaca gaggtttaac
      181 tacaagagaa agaaaatttt acggcgtacc ggatttggta gtacggcttg atgattacat
      241 aaaacttcct gttatgtatg aggtattttt cgagctggtg ccaggtgtgc gattcaggga
      301 aagaaaatca ggtgcagaaa tgagagtgct ggaaccgctt tcgaacatat tctataatga
      361 gcctccactt gcaatgatag acggtgtgat aattcaaaac ttatctctca ttgccaatct
      421 cgaccctgaa attgttgaaa agattgaact tgtacgttca agatatatga ttggcgacct
      481 ttatttcagt ggaattgtta atgttattac gcgcaaaggt gacctgagca acattgtact
      541 tcctgattat gcagtgaata tcaggtacag ggtaacggaa aatcctgctt cgttttcacc
      601 cccggattat tctacagaag aaaagaaaga aagtcgcata cccgacctta gaaacaccct
      661 ctactggaac ccttctgcca tacccggtga acagggtgtt gtgacagcag aattctggac
      721 acacgatcag gcaggagaat attcaatagt tgccaccggg gtgacacaat caggaaaact
      781 tatctcgacg atacactctt tcagaataag gtagaaacgc tgattaagtt ttttggtacc
      841 tgcttcacta atgttttatt gtcattatcc agatttaatt ctaaagtttt agttctattt
      901 cttcgatatt ccatccctca accttgagaa cagctttttt gcctgatatc ttctcaggtg
      961 ggcacccaac tgtaacagat attgattcac caggtgccag tgtaaagtag tttgccgacc
     1021 agaatgaagg tgttatttcc tcattatctg ccataagcct cgggttgata aaaaaggcaa
     1081 ttttatccga tgaatttgtc agcgtaaagc tccatttcag ttcatttgac cctttcagta
     1141 caccggcaag cttaacatta acttttgtcc gtggcataga gttgagggag gtaaaatcat
     1201 tcgatgagga ataccagtaa gcgttgtgag agagcctctt gcccgaattg tcgtaaaggt
     1261 taagaattat gaatgaaact cctgtgtttt ttgaaagtgc atctgaaaga gagaggcttc
     1321 cggctgatga aggaccttca agacttactg tttcattctg actgtaaatc agtttcgatt
     1381 tcaaatcaaa tatttttatt tccgacttca gatttgaaaa tgacttatac gttctattga
     1441 cgtactcgac cttcagatct ttcgggttaa actgaacgtg tacaggttcg caggcattct
     1501 gcatgaaata gtatcctgcg ttgggcataa ggtaccagtc atatacctgc cataccacac
     1561 tcgggaaggc agcgtttagt ttccagagca ttactcctcc aacatcattc agtttatggg
     1621 caggagcttc gaatattgcc tggtatcctg aagcattcat aagctgcatc ttgtcggaga
     1681 attccttcat tgttgatgtt gtgccgtaac gttttatcat ttcattataa taccgatcgt
     1741 atcgtccgtt accggttgcg gcatcgtgat agccccatga atggttcagg gggaagggaa
     1801 gtgatttatc ccatacaagg ttggtgatta ttttgggcaa tgtgctgtaa ggtggctggg
     1861 aaagtatgcc tgtttcgtcc ttaaaaaccc agtcggcggc agcatccacg cggcggtagt
     1921 aatcgcgggg gtcctgccag gtatatggtc cgccgctata tacaccgcca ggcaggccat
     1981 caggccagga ggcatcccat ccttccggca gacgcgcaaa gcctgatgag catggaataa
     2041 acgggcgggt tccatcaagt tttattacac tgtcgcgcat ggcataatag agttcccttc
     2101 gtgcatgccc ttcgttgcct ccggtccaca ccagcaggct cggatgattt cttatcctgt
     2161 aaatagtgct tgtcacattc tttttgaata tctccccgtc gagtggccag tcgggtgacc
     2221 ccttgaattc accctgagta tcacctgtta tccagaaatc tgaccatacg agcaagccgt
     2281 aacggtcggc agcatcgaaa aaccagtccg gcggtgtcac tcccccaccc catattctta
     2341 ccaggttaat atttgcattg cggcatagcc ggaactcata atcgtaccgc atcgaatcac
     2401 ggttgagcat catatcgggg acccaggcac cgcctgttat atggatacgc cggccattaa
     2461 catagaaatc gcgccgccac atcttgccta cctgcacagc ttttgaacca actgtcctga
     2521 tgccaaacaa aaaggaaatc tcatcactca gacccgactg gttcgagtac tgaatcctta
     2581 ttctataaag gtcggggcga ccgtaaccgt tagtccacca taacgccggt ttgctgaaga
     2641 gaagctctct ggtgttatct gcgtcataag caacaactaa agaggagttt ccattcaaag
     2701 agacatcttt tgtaaactgt acaggcttac cctgaaaggt ttctggtgta attatcactg
     2761 ttatctttcc tttttcggta gacttaccgg aatttttaag tttaaatgaa agggaaattt
     2821 ttgcaagtgt tgtatcgggc aggttgggta gaagagtaac gagctttggt tgttctattg
     2881 taacgtgtcc cgatgttctg agatagacag gttgccagat gccgatgtta cggtcgcgta
     2941 ctggtggtat ccagtcccat cctaccgagc acagcattgt tacgtttttg cctatgtcac
     3001 cagtttttcc accgttgagg taaaagtcgc ccagcgcttt caactgttca gtatcgggca
     3061 agcccgggta atcgagaggg tagattttca cggcgagggc attttttgag ccggggataa
     3121 tacaatcgct aacgtcaagc ctgtattcgg caaacatacc tgccatctga gtagagtcgg
     3181 ctatctgttt gccgttgagc cacacaccag ccctgtagtt tattcccttg aatataagtt
     3241 cgaagtggcg gcctttatct tcagcaggaa ccatgaactc tgtacggaac cagtaaggtt
     3301 tgctccatgg gttgggcaga ccgggtagat ggctatactg ttcaaggtta tattgtttat
     3361 tgaattcatc tgaagcatcc ggtatgagca tattgttaag cccaaggtag ggatcgggat
     3421 agattttgtt tgccacaagg gctgagagta ctgttgttgg tactttaaca ggcatccacc
     3481 atatacctgg gcggaagccg gggtcggaga gttgctcgcc cgtttcagat acaacgaatg
     3541 aagattgcat tacaaactcc gagagccgtg caattttcac cctgtcgttc tgtgctgaga
     3601 gcctcaaaga aaagagcgct acagtaaaaa ttacaactga tataaaggct gatgttttca
     3661 tgattttatt ttacaattat ttatcaaaat gaaataactt ggcatcgttt ttaagattgg
     3721 cagataaatc gtaatcatat acgcaaatat atattttaaa aatgaaacgt attcttgtcg
     3781 acatggacgg ggttctggct gatgtgtaca gccgtttttt tgagcttcac gagaaggaga
     3841 ctggtgttcg tctgaccgtt gaagaggtgg caggcaagct tgaggctgaa gcatttccgc
     3901 atcagataaa atgggtacga cgcctggctt tttcaggagt gtacctgtga tggagggtag
     3961 tgttgaaggg ttgcgaaggc tgaatgaaaa ataccatgtt gttgtcattt cactggcgac
     4021 tgaatttccc agcagtctca ctgacaagca gatgtggttg catgagcatt ttccttttat
     4081 aagctggagg cagatagttt tttgtggaga taaaagtttg attaaagcgg acattatgat
     4141 agatgatcat ccgaagaatc tggatgtttt tcagggaggg actataattt ttacccagcc
     4201 tcacaatatg ttgctggaaa cgaggcatcg tagggtttcg acatggaagg agatagagga
     4261 aattctttta taatacaaaa agctgtaatc agataaaaat taagtattca ggaaccagtt
     4321 ttcagagtgt tgaattatta ttttagaaat cttactattt gataattttt attatctgat
     4381 attttttatt gtttcaaaca ttttactatt tgatattttt tactatttga taaattatac
     4441 tatttaaaaa tttttattat ttagtaaatt ttactattta aaaatattta ttatctgata
     4501 atttttctaa ataagataac gggattcaca acaatccagt ctgactcacc aaaccttcaa
     4561 aaaataaaca gaaaaaataa aaaatccggg atgtaacctc tataataaga tacaccccgg
     4621 aataaatttt agcgtctggc ttataaatcc agcctcaaca gatctgtatt ccagctttca
     4681 ataacaagta aaggcttctc ccctttcaac tccttaacat caaattcaac attcaccacc
     4741 ctcttttccc ccggaagcag cgaaaagtaa ttgtcatccc agaaaaccgg taaaaccggg
     4801 ccttttgagg aacttctcaa aattttcgga ttaactccaa aggcaagatt cttacctgtg
     4861 ttttccagcg ttactgtaat gcgtgccctg ttaccttttt gctgtaaagg agttgcatca
     4921 actttaagat tcgtcttcgg aagattcatt aagtctttga aatctgcctt ctcatcacca
     4981 tcagcatgga gccagtaaaa attcgatgag acaggcttcc cttcggcatc tttcatctcc
     5041 agcctgagga agtaaatctt cgttatattt gccggcacct caactgcaaa aacatttttg
     5101 ctttcatccg gttcaacatc agcctgtgca gtttttgacc atacctccct ggagttgaaa
     5161 tcaaaaattc tcgctgacac cttcagcccc ttaaattctc tgtacagtga attgacaatt
     5221 tttactgtat gttctttgta tgagtatagc acatgcagag gttcgcaggc actccgggct
     5281 gcataaaagg ccccgtttgg catatagaaa taatcgtaaa gctgccagta catctttggc
     5341 caggcaccat tgtacatcca gtaaataatg ccactcgatt tatatttgtt tgccgcatat
     5401 gcttcgaaca ttgccttaac agcttcattc tgaaatatct gtgccttaag gctgtattcc
     5461 tcaactgatg agggttttcc ataccttgat tcaaatgcag ctctggctat tgggtagaaa
     5521 tttttgtgca gcctcagctc ccacgactca cttggcggcc agaggtcttc ttcaggcatc
     5581 atcatcctca tggtctccag agggggtatc tgttcacctg acggccctgc ttcggtgttg
     5641 aattcaagct tgctgtacca gtaggaaggt gggaagtatg cataaaccct tggataggga
     5701 cccatccaga cacctgtcgg acctgcaatt ttgcttgcag cctgcgtggc tgaggataaa
     5761 tacggtcttg tcccgtcgta ctcatccagt acttttatgt atcttctttc aacatcttca
     5821 ggcggataat tgtcgctgcc atatagccag acaaacaccg aaggatggtt tctcagatgc
     5881 actatctggt ctctccagct cagttcggca atatcggccg tatgaggtgt ccaggttttc
     5941 catctctccc acatgctgca gcagcaccat cctaccatca gaaggatacc ttcctcatcg
     6001 cacctgtcaa acatatagtc tgaccccctc ggcgcttcca tcctcagtgc attcagattc
     6061 atatgttttg cgtaagcaat atcggcatca atcctttcat tggaaggttt gagaaacaga
     6121 tcctgaacgt acccaccgcc ccttataact atgttcttac cattaacctg aaaaactctc
     6181 gtcttcagcg aatcaaattc attcatccat gatgtgatct cgcggattcc aaacctcact
     6241 tttctcatat ccgacaccct gcctgatgct tcaaaagaaa ggttaaggtc gtaaaggttc
     6301 tgaggaccaa cattatgagg ccaccacaga cgtgggttct ttattatcaa ctgacggaat
     6361 tcagagggtt caaaaacaac ctttttcgtc tctcctgcat taaggcttac ctgctttgaa
     6421 aaagttatct tttctatctt acccgtcagt tttccgctta ctggcttact gaatgaattc
     6481 ctgagcaaaa ctgaaacagt aagtcttgct tcatcgttcg acggaatatt tagttttgtt
     6541 ttaacaaaag gttcctcaat tgacacgtct cccgtggcat gtacagttac atcataccat
     6601 atgcctgtcc cgttgtcagg tggtgtcgga ttccagtcaa cccacgtgat agaaaggtct
     6661 gtgcttttgg gagggaaaat ttcgagtgcc aggcagttgt taccgcccgg taaagcttct
     6721 tttgtaatat cgagtgtaaa gagcctgtat gcaccctcaa tggttgtggt atcagccaca
     6781 agttttccgt ttagccagat attggcttta taattgatac tatggaagtt aatccatata
     6841 tgcttcccct tataagcggc aggcagctga aattcagtac gataccacca tgctacgctg
     6901 aagggactgt cggtcggaat actcctgttc cggtttgata tctgcccggg aatcttgtta
     6961 aaattagtcc cgtaataagg atttgtgatt acattatttg cttc
//
LOCUS       DAOSNB010000116         7004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10806_length_7004_cov_3.982587, whole genome shotgun sequence.
ACCESSION   DAOSNB010000116 DAOSNB010000000
VERSION     DAOSNB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10806_length_7004_cov_3.982587"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3057
                     /locus_tag="PLG42_05945"
     CDS             <1..3057
                     /locus_tag="PLG42_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU30784.1"
                     /translation="YSLVQLPVDLYPEIELPYITVLTTYPGTSASDVETNVTRPIENA
                     LNSVGNLKELTSTSSDGLSVVFLQFEYGSNLDEAMNDIRSNLNFVRRTLPDGCEEPTI
                     IKFNSSMMPIIFYAITAKESYEGLEKILDERVVNPLNRIDGVAAVNLSGVPGRRIYID
                     IDPQKLEAYHLTLEQVGNVLRSENINMPAGSLEMGQSDYALRVQGEIPESDLLRDLVI
                     SNYNGVTVYLKDIAEVRDTIRESKFDTKINRSFGMGLYVQKQSGANTVKITKEIEKTI
                     ESVKKDLPPDVKIEKLFASATFIQDAIRNLSETLLFAAIFVVLVVLFFLGRWRATFII
                     ILTIPVSLVVAFIYLFVANASINIISLVSLSIAIGMVVDDAIVVLENITRHIERGSRP
                     REAAIYATNEVWLAVIVTTLTVVAVFFPLTFVKGLTGVLFKQLGMSVTIVITTSTLAA
                     ISLTPTLSALLLKYTPIKPDAPFWTYDGSIRKGLDWFDKFYEKTLRWALRHKTFVIIM
                     SFTVFISSMALFAFVPTEFFPQADESRIMTTVELQTGTRVENTIKVADRLDSIFQAKY
                     PEVNLISTSTGSDDQGGWASIFSAGGTHVIRQSIRLVPLEERQRSVWDIAEEMRKDIS
                     AFPEVINFTISTTASMGSFGGNTVEVEIYGYDIAATNIVANELAEKIKKIPGAKDVAI
                     SRGKSKPELQIIFDQNKLSASGLNTAMAASALRNRIGGLTATQLRQAGDEYDIIVRFR
                     KDARNTLTKIENIGIPTPSGQLVRLGEVAQIKEYWAPPSIERKRKERVVRISLTPYKR
                     SLTALQMDIQKAIDSTPKPAGVMVQISGAIKEQMEAFKDLAMLLVVSLILVYLVMASQ
                     FESLRMPFIIMFSIPFAFSGVAIALFLTGTALNVISGIGAVLLIGIVVKNAIVLVDFI
                     NLMRGRGLELYEAIAVSGKSRLRPVFMTSATTILGMFPLALGLGSGSELWSPMGVAVI
                     GGLIFSTFVTLVLVPVVYAIFVKKGIERRKRAEYADINFLNGNGQV"
     gene            3084..3377
                     /locus_tag="PLG42_05950"
     CDS             3084..3377
                     /locus_tag="PLG42_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOU30785.1"
                     /translation="MKAIMIVYNQALTEKVEYMLDKLSIRGYTLWENVQGRGTSTGVP
                     HLGTHVWPEINKSLLTVVEDDKVDRVLEAVKKIDNINEEVGIRAFVWDVIKTV"
     gene            complement(3449..5605)
                     /locus_tag="PLG42_05955"
                     /pseudo
     CDS             complement(3449..5605)
                     /locus_tag="PLG42_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            6032..6409
                     /gene="rpsL"
                     /locus_tag="PLG42_05960"
     CDS             6032..6409
                     /gene="rpsL"
                     /locus_tag="PLG42_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOU30786.1"
                     /translation="MPTIQQLVRKGREKLIDRSKAPALDSCPQRRGVCIRVYTTTPKK
                     PNSAMRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DASGVEGRSQRRSKYGTKRPKKQ"
     gene            6437..6913
                     /gene="rpsG"
                     /locus_tag="PLG42_05965"
     CDS             6437..6913
                     /gene="rpsG"
                     /locus_tag="PLG42_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOU30787.1"
                     /translation="MRKARAKRRILPPDPKFNDPLVTRFVNNLMKNGKKTVAFRIFYD
                     SLNIIGEKLKVEGKTPIDIWKQALENITPQVEVKSRRVGGATFQVPVEIRPERKVSVS
                     MKNLIFFARQRPGHSMAEKLAAEVIAAYNNEGGAFKKKEDTHRMAEANKAFAHFRF"
BASE COUNT         1982 a         1481 c         1605 g         1936 t
ORIGIN      
        1 tattctttag tacagctccc ggtggatctt tatcccgaaa ttgaattgcc atatatcact
       61 gtacttacca cttatccggg tacaagtgca tcggatgtcg aaacaaacgt aaccaggccc
      121 attgaaaacg ctcttaacag cgtgggtaat cttaaagaac ttacatcaac atcgagcgac
      181 gggctttcgg tggtctttct ccagtttgag tacggaagca acctcgatga ggcgatgaac
      241 gacatcagaa gtaacctgaa ctttgtcagg aggactcttc ctgacggctg tgaggaacca
      301 accattatca agttcaactc gagcatgatg cccataattt tctatgcaat aactgctaag
      361 gagagttatg aagggctcga gaagatactt gatgagaggg tggttaatcc gcttaacagg
      421 attgatggtg tcgctgctgt gaatctttcg ggcgtaccgg gcagaaggat ttatattgat
      481 attgaccccc agaaacttga agcttaccac cttactctcg aacaggtggg aaacgttttg
      541 aggtcggaaa atataaacat gcctgcaggt tctctggaga tgggccagag cgattatgcc
      601 ttgcgtgtcc agggcgagat acccgagagt gacctgcttc gcgatcttgt tataagcaat
      661 tataacggtg taaccgttta cctgaaggat atagccgaag taagggatac tatccgcgaa
      721 tcgaaattcg acacaaagat caaccgcagc ttcggcatgg gactctatgt gcagaagcaa
      781 tcgggtgcca atacggtgaa gattacaaag gaaattgaaa agactattga aagcgttaaa
      841 aaggatctgc cgcccgatgt gaaaattgag aagctctttg ccagtgctac attcatccag
      901 gatgcaatac gcaacctgtc ggagacgctc ctttttgctg caatcttcgt tgtgcttgtt
      961 gtgcttttct tccttggaag gtggagagct acatttataa taatattaac aataccagtc
     1021 tcactagttg tagcattcat atacctgttc gttgcaaacg cctcaataaa tatcatatca
     1081 ctggtatcac tctcgattgc catcggtatg gttgttgacg atgccattgt agtgcttgag
     1141 aatataacgc ggcacattga gaggggcagc cggccgcgcg aagcggctat atacgcaaca
     1201 aacgaagtat ggcttgcggt tatcgtgaca acacttaccg ttgtagccgt cttcttccct
     1261 ctaacattcg taaaaggact taccggcgtc ctgttcaagc agcttggtat gagtgttaca
     1321 atagttatta ccacctccac actggctgcc atttcgctta caccaacact tagcgcactt
     1381 cttcttaaat atacccccat taaaccagat gcacctttct ggacatacga tggcagcata
     1441 agaaaagggc tcgactggtt tgataaattc tatgaaaaaa cactgcgctg ggcactgagg
     1501 cacaaaacct ttgtgattat tatgtctttt acagttttta tttcgtcgat ggctctcttc
     1561 gcattcgttc ccactgagtt tttccctcag gccgacgaat cgagaataat gacgaccgtc
     1621 gaattgcaga ccggtacacg ggttgaaaac actatcaagg tagccgaccg gctcgacagc
     1681 atcttccagg caaaataccc ggaggtgaac cttatttcaa cctcaaccgg ttcagacgat
     1741 cagggtggct gggcttcgat attcagtgcc ggaggtactc acgttatacg ccagagtatc
     1801 agattggtcc cgttagagga aagacaacgc tctgtatggg atattgccga ggagatgaga
     1861 aaagatatct ctgcatttcc tgaggtgata aactttacga tttcaaccac tgcaagcatg
     1921 ggaagcttcg ggggaaatac cgtagaagtg gagatttacg gatacgatat tgcagccaca
     1981 aatatcgttg caaatgaact tgctgaaaag atcaagaaga tacccggagc gaaggatgtt
     2041 gcaataagtc gaggaaaatc aaaacctgag ctacagatta ttttcgatca gaataaactc
     2101 agtgccagcg gactcaatac tgcaatggcg gcttcagcat taagaaacag aataggcgga
     2161 cttacggcaa cccagctgcg ccaggcaggt gatgaatacg atattattgt cagattcagg
     2221 aaagatgcaa gaaacacact taccaaaatt gaaaacattg gcatacccac gccatcaggt
     2281 cagcttgttc ggttgggtga ggttgcacag attaaggaat actgggcacc gccaagcatc
     2341 gaaagaaagc ggaaggagag ggtggtcaga atatcactta caccatacaa gcgttcgctc
     2401 actgctcttc agatggatat acagaaagcc atcgattcaa caccgaaacc tgctggagta
     2461 atggtccaga tatcaggtgc cataaaggag cagatggaag catttaaaga tcttgctatg
     2521 cttctcgtgg tcagccttat tcttgtctac ctggtaatgg cttctcagtt tgaatcactc
     2581 aggatgccat tcatcatcat gttctcaata cctttcgcct tttcaggtgt ggcaattgct
     2641 ctcttcctta caggtactgc ccttaacgtc atctccggta tcggtgcagt actactgata
     2701 ggtatagttg taaagaatgc aattgtgctt gttgacttca ttaaccttat gcggggaaga
     2761 ggactggaac tgtatgaagc tattgccgtt tccggaaaat cgagactgag gccggttttc
     2821 atgacttctg ccacaacaat tcttggcatg ttccctcttg ccctcggact gggttcaggt
     2881 tctgagctct ggagtccaat gggtgttgca gttattggag gcctgatttt ctcaactttt
     2941 gtgacacttg ttcttgttcc tgtggtatat gccatctttg taaagaaggg aatagagaga
     3001 agaaaacggg ctgagtatgc cgatattaat ttccttaacg gaaacggaca ggtctgaaat
     3061 acaaacttta aaacatattg attatgaaag cgataatgat agtatataac caggctctca
     3121 ctgagaaagt agagtatatg ctcgacaaac tgagcatcag aggctacaca ttgtgggaaa
     3181 atgtgcaggg acgcgggacc tcaacaggtg ttcctcatct cggaactcat gtatggcccg
     3241 aaattaacaa aagcctcctt acagtggttg aggatgataa ggttgacaga gtactggagg
     3301 cagttaaaaa gatcgacaac atcaatgaag aagttggcat aagagccttt gtgtgggatg
     3361 ttataaaaac tgtttgatag ggataggcat tggaaaaaag aaagccgttc atttgtgaac
     3421 ggctttcttt atattattag gatagtaatt attcttcctc ctgttcggcc tggtaagcct
     3481 tgataacagc ctcctggacg tcgggtggaa cctgagcgta ctcggcaaat ttcattgtat
     3541 agaatgctct tccgccggta agtgaactca gtgcagtgga gtatttgttc atctcagcaa
     3601 gcggtactct tgccttgatc acttcaaacc tcttttcgct cgacataccc agaaccattc
     3661 cccgcctgct ctgaaggtcg cttattacat cacccattct gtcggaagga accattatct
     3721 caacatcata tataggttcc agaatcttcg gggctgcatt tttgaaggcg atgctgaaag
     3781 catttcttcc tgccagcctg aaggagatat cgttcgaatc gacagggtgc atcttgccat
     3841 cgtatacgta aactctgata tcccttgcat aggagccggt aagcggaccg acttccatct
     3901 tttccattat tcccttaagg atggaaggca taaagcgtgc atcgatggaa ccacctacta
     3961 tgcagtttac gtatatcaac ttaccgcccc acggcaggtc gatctcttct ttatcacgta
     4021 ttgaaagtcg atattctttt ccgccgatct taaagaatga tgattcggta gaaccttctt
     4081 cgtaaggctc gataattaga tgcacttcac cgaactgtcc ggctccaccc gattgtttct
     4141 tgtgcctgta atcggcctgg gctatcttag ttatagtttc acggtaagga attctcggcg
     4201 ggttgaatac tacgggaatc ttataaatat tgtcaagatg ccatttaaga atatttaaat
     4261 ggtattcacc ctgtccatga actatgatct gtttcagctc tttcgagtat tcaactatga
     4321 ttgtcgggtc ttcaacgtgc atcttgtaaa gtgcttcgcc cagtttttca tcgtcccctt
     4381 ccgaaactgc cttgatggct gtgcggaatt tcggctcggg gaattctatt cctttctaag
     4441 tccagtcgtt gcccggtgca ttaagtgtat gattggtttt ggtgtttttg agctttacaa
     4501 cggcaccgat atcaccggta tgcagctctg gaacttttgt cctgttcttg ccggcacaca
     4561 caaaaagctg ggcaagcctt tcctttgtgc cgttgctggt atttatcata tcaatgtttt
     4621 cggtgagttt tcccgaatat actctgaaat agttgatttc accgatatgt tcttcaattg
     4681 atgttttgaa cacaaaaact gaagctggcc ctgcggggtc gactttaaca ggctggccgt
     4741 tgctgttaag aggtgcaggg acatcagtga cagtgggtac ttcagatact atgaaatcca
     4801 tcaggtcgtc aacaccgata ttgttctttg ccgagatgca gaacaccggg aacattgtac
     4861 gggcaaggag gctctttgtg ataccttttc ttatttcagc ttccgagagg gtgtcgtttg
     4921 aaaagaagag ctccataagt gattcgtcac actcagcagc tttttcaacc agtactgaat
     4981 gaagttctgc tgccctgtcg gcctggtcgg caggaatatc gagaacctca gctttacctc
     5041 cgtcttttcc gtatttcagc atcttcatct tcaacacatc aataacagca ttgaatccga
     5101 caccttcgtt aacaggatac tgtacaataa caacatcatt actgaggcgc tctttcatca
     5161 tttcgatcga cttttcgaag ttcgccttct catggtcgag tccgttaatc accattatca
     5221 ccggtttgtt tagctgttcg gcataacgga aatggatttc ggtacccact tccacgccgt
     5281 tctggacgtt gataagcatc aaggcgcagt cggatgcaaa aagagatgaa attacacctc
     5341 cacagaaatc atcaagtccc ggtgcatcga gtatattgat ttttttgttc tgatattcgg
     5401 tataaagaac tgttgagaaa attgagtttc cgctttcctg ttccacaagg cggtagtccg
     5461 aaacggtgtt tttagattca actgtacccc ttcggtcaat aactcctccg ttaaagagca
     5521 ttgcttcggc cagggttgtt tttccggaat ttgaattacc aatcagcgaa atagttttaa
     5581 tctggtcagt tgtgtatgtt ttcattctgt aaaattgtta ttgatgttat acattatatt
     5641 atatatatca attgggagaa atgaggggac aaaattaata attttttaaa atcttccctt
     5701 gtaatgcatt ataattcggg aaattttatt ttctttgcac cagttttttg aaaagaaaca
     5761 ggagtaccgg tgtttccctc ctatgtaagt ttacaaaaga attcaatcgg gaaagatcgg
     5821 gaaggaacct gctgataact tacacaagaa gtaagtaaat agtaactgaa gtaattgcaa
     5881 atgccgggct ggcatttatc tgttcggctt ttgcgtgatt atatatattg tttttgaaaa
     5941 aagtaaaata acgatttgca aaaaagaaaa taaaattagt aactttgcag cccgtttttt
     6001 aatgaaaaat taatgttaaa caattgaatt tatgccgaca atacagcaat tagtaagaaa
     6061 aggcagggaa aagctgatag acagaagcaa ggctccggct cttgattctt gcccgcagcg
     6121 aagaggtgtt tgtatcaggg tttataccac aacacccaag aagcccaatt cagcaatgag
     6181 aaaggtggca agggtgaggc ttaccaacca gaaggaggta aatgcctata tcccgggtga
     6241 aggacataac ctgcaggagc actcaattgt tctggtaaga gggggtaggg ttaaggatct
     6301 gccaggagtc agataccata tcgtaagagg tactctcgat gcttcaggtg ttgagggacg
     6361 ttcccagcgc cgttcgaagt atggtactaa gagacccaag aaacagtaat gaagtttaag
     6421 atttaagatt aagataatga gaaaagcaag agcaaagagg agaatattgc cgccggatcc
     6481 gaaattcaac gatccgctcg ttacaaggtt tgttaataac cttatgaaaa acggcaagaa
     6541 aacggttgct ttcagaattt tctacgattc gcttaacata attggcgaaa agttaaaggt
     6601 tgagggcaaa acacctatcg acatctggaa acaggcgctc gaaaatatta ctcctcaggt
     6661 tgaagtaaag agtcgcaggg ttggtggtgc cactttccag gtgccggttg agatcagacc
     6721 cgaaaggaag gttagcgtca gtatgaaaaa cctgatattt tttgcacgcc aacggcccgg
     6781 tcattctatg gccgagaagc tagctgcaga ggtgatagcc gcatacaaca acgaaggcgg
     6841 cgccttcaaa aagaaggaag acacccacag aatggctgaa gccaataagg catttgctca
     6901 tttcaggttc tgataaaagg ctgaatacca cagacaggaa tggataaaaa cctgaaatat
     6961 accagaaaca tcgggataat ggcccatatc gacgccggta agac
//
LOCUS       DAOSNB010000117         6990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10841_length_6990_cov_4.039942, whole genome shotgun sequence.
ACCESSION   DAOSNB010000117 DAOSNB010000000
VERSION     DAOSNB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10841_length_6990_cov_4.039942"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="PLG42_05970"
     CDS             <1..1070
                     /locus_tag="PLG42_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOU30788.1"
                     /translation="IENSDEKNRLKTLVQSIKPQNYGVIVRTAAEGKKVAVLDAELRE
                     LVKKWESAFASIRRDIKTPRLFIGEMSRITTILRDLLNVSFNSIQVNDEALLNEIKRY
                     ISEIAPEKENIVKLYKGNLPIFDHFGVTKQIKALFGRTVSFWHGAYLIIEHTEALHVI
                     DVNSGNRARTGTDQETNSLEVNLAATKEIARQIRLRDMGGIIVIDFIDMQSAENKQLL
                     YEKMKEAMQNDRSKHNILPLTKFGIMQITRQRVRPEITVVTNEKCPTCRGTGEIKSPL
                     LLIDEIETSLTLVLEKIKTKKLVLKVHPFIEAYLNKGLISTRMKWNLRYKICLKVIAI
                     PSYHLLEYHFFDRYENEIDVT"
     gene            1171..3135
                     /locus_tag="PLG42_05975"
     CDS             1171..3135
                     /locus_tag="PLG42_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOU30789.1"
                     /translation="MKKFGLLLLLFFLVFSNTNSQVHDPVKWSFGYEKKNDNRYEIIF
                     KALIEKGSHIYSMSVPPDGPVPTTVSFESSTLYSLEGKPFEVTEPVEKFDEAFGFKIK
                     TFSDSAEFRQVIVSSESNFTVKGTITYMSCTDAVCLPPNDIEFTVSVGEAGSENTETV
                     APSDIESGKKGIFGFFIASFLLGLVGLLTPCVYPMIPMTVAFFTREKDNRRGAVLNAL
                     IFGLSIVIIYTLPGLIITLTGAGAGFAGELSTHWIPNIVFFILFVVFAISFFGAFEII
                     LPNRWVSSADSKVDRGGLLASFFLALTTVIVSFSCTGPIAGSLLVEAASGDIIRPTIG
                     MFAFGLAFSIPFTLFALFPSVMSKLPKSGAWLNSVKVVLAFLMLAFSMKFLSTIDSVY
                     GLNIISREIFLAVWIVIFSLTGLYLMGKIRFPHDSEVKHISVFRLFLIIVVFSFVLYM
                     IPGLFGAELKGLSPLLPARKSVSSLSPVQYQGYNSELKSVTNTDSNIGCSQPKYADRF
                     QLPYGLTGYFEYNQGLMCAKEKGSPVLIDFKGHACANCKLMEAKVWSDPRVQERLRKF
                     VIIALYTDDRTQLPEEEWITSSVDGKVKKTIGKINEDLEIMKFKTNAIPLYVITDYNG
                     NPLVKPVATTLKVEEYIEWLDEGLRRAGVN"
     gene            complement(3146..3658)
                     /locus_tag="PLG42_05980"
     CDS             complement(3146..3658)
                     /locus_tag="PLG42_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30790.1"
                     /translation="MKKVVWIQVLLLICTFYSLQGQGVFRQAGLRAGYTGGLFFQSAS
                     PSGNADAGVMALLSFRTNGLQLTGMKIFYDNALSEIILDLFTGWGYGAHAGFITEYVT
                     VYEKKDYYYKTERLCPLIGIDVWGTLEYRFRDMPLAVSLNIKPFFEVGLPSFFRLMPA
                     DLGISVSYMF"
     gene            complement(3734..4834)
                     /locus_tag="PLG42_05985"
     CDS             complement(3734..4834)
                     /locus_tag="PLG42_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30791.1"
                     /translation="MKMTETSVFNKLLLTLLCIGTTVNSGAQFTQKRIFERKEPVTRE
                     TTLEINNKYGTVEVTVWKKDSVSIRAEIEAQAPNQERTDKMIKGINIEISSTKYFIRA
                     QTEFEQSLNMILENFKGMTKKLIPYDSKVQINYYIQAPPYMNLRIENRYGDIYVDQNS
                     NDVNISLSNGSLKANTLSKISQLKVSFGNVVIKNAGDCNIDGSFSEFIIDESDHIRVK
                     SISSRYDIKQVKTLLVESRRDKFYIGTATTLTGDSYFTQLRVNRLLSDTDLSARYGDT
                     YFELIENSFRTINVTSSYNDLTLNFEPSASYNLEIRNTGTSVILPSGNADIKREVLNE
                     EKREYIHTGIVGRNPDSRVVKINATRGTILIR"
     gene            complement(4806..5318)
                     /locus_tag="PLG42_05990"
     CDS             complement(4806..5318)
                     /locus_tag="PLG42_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30792.1"
                     /translation="MDKLEKFIKENRDKFDIYTPHGSVWRKIRRETGVRTFYWRAAAA
                     IAVIITGLSMLYFITKPEKPLTGSSSERELNEAIVFYSVRFNELFSTAEPLLNTRPEL
                     RRELYADMERLDSIMNDIKKDLKDRVANSEVIEALIQNYRSRVLILEEMLDILKENEK
                     ENENDRDQRL"
     gene            complement(5311..5871)
                     /locus_tag="PLG42_05995"
     CDS             complement(5311..5871)
                     /locus_tag="PLG42_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU30793.1"
                     /translation="MTGNADDREQEAVAFLIEECKKGNHRAQYRLYRQYSKAMYNLAY
                     RMTNSREDAEDILQETFIECFRNLSSFRFESTFGGWLKRILINKSINHLRKKKLALVF
                     PETLPEVMEDNYDEGETEYDVSAVHKCIELLPDGYRIVLTLYLLEGYNHSEIAQILGI
                     TESTSKSQYSRARAKLKDLLKNRMYG"
     gene            6123..6614
                     /locus_tag="PLG42_06000"
     CDS             6123..6614
                     /locus_tag="PLG42_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30794.1"
                     /translation="MKRLIFLLISINLVFSAVAQVKQEVIASAGGYNKSADNTISISW
                     TLGETIVPTYVSQDGSLILTHGFQQKLIITSIEENIEVPVTVTVYPNPTSELLNIKFE
                     APVDKLIHVQIMDAQGRIVKNDRIEVSVLQKTINLQDLSPGVYFMRLVKGTLINVYKI
                     VKL"
BASE COUNT         2058 a         1337 c         1423 g         2172 t
ORIGIN      
        1 agattgagaa cagtgatgaa aagaaccggc tgaagactct tgtccagagt attaagcctc
       61 aaaattacgg ggtaattgta aggacagccg ccgaaggtaa aaaagtggct gttctggatg
      121 ctgaactcag agagctggtc aagaaatggg aaagtgcgtt tgcttctatt cgaagggata
      181 ttaagacacc acgcctgttt atcggtgaaa tgagcaggat aactactatt ctgcgtgacc
      241 tgcttaatgt ctcttttaac agtatccagg ttaatgatga ggccctactg aatgaaataa
      301 aaagatatat atcagaaatt gcaccggaaa aggagaatat tgtaaagctt tacaaaggga
      361 atcttccgat tttcgaccat ttcggtgtta caaaacagat taaggcactt ttcggacgca
      421 cagtatcttt ctggcatgga gcctatctga ttattgaaca tacggaagcg ctgcatgtga
      481 tagatgttaa cagcggcaac agggccagaa cgggaaccga ccaggaaaca aattctctgg
      541 aagtaaacct ggctgccaca aaggaaatag cccgccagat acgtctcaga gacatgggcg
      601 gaataattgt catcgatttt attgacatgc agtcggccga aaataaacag cttctgtatg
      661 aaaaaatgaa agaggcaatg cagaatgaca gatccaaaca taatattctg cctcttacta
      721 aattcggaat catgcagata acccgccaga gggtaaggcc agaaataact gtggtgacca
      781 acgaaaaatg ccctacctgc agaggaacag gagaaataaa atccccgctg cttttgatag
      841 atgaaataga aacaagcctt actctggttc tcgaaaaaat taaaacaaaa aaactggttc
      901 tgaaagttca tccgtttatt gaggcatatc tgaataaagg tcttatatca acccgcatga
      961 aatggaatct gagatacaaa atatgcctca aagtgattgc cataccctca tatcaccttc
     1021 tggaatatca tttcttcgac agatatgaaa atgaaataga tgtcacataa ctgatgaaca
     1081 tttttgcttg aaatctgttt gacagttatt tgcatcactt taattccttt atcgtacatt
     1141 tgtgaacgtc ataccatttt gatctgatat atgaaaaaat tcggtttgtt attattgtta
     1201 ttcttcctgg ttttctcaaa tacaaattcc caggttcacg accctgttaa gtggagtttc
     1261 gggtatgaaa aaaagaatga caacagatat gagataatat ttaaagcatt aatagaaaag
     1321 ggttcccaca tctattcaat gagtgtgcca cctgatggac ctgtgccaac aaccgtcagc
     1381 tttgaatcat caacccttta ttctctcgaa ggtaaacctt ttgaggtcac cgaacctgtt
     1441 gaaaagttcg atgaggcttt cggttttaaa ataaaaacat tcagcgacag tgctgaattt
     1501 cggcaggtga tcgtatcttc tgaaagtaat tttacagtaa aaggaactat aacttatatg
     1561 tcgtgcaccg atgcggtgtg tctgcccccg aatgacatcg aatttacagt ctcagttggt
     1621 gaggccgggt cagaaaatac tgaaacagta gccccttctg atattgaatc agggaaaaaa
     1681 ggaatctttg ggttttttat tgcttctttt ctgctcggac tggttggtct tctcactccg
     1741 tgcgtttatc cgatgatacc aatgacggta gcattcttta ccagggagaa agataacagg
     1801 cggggagcag ttcttaatgc attgattttc ggattgtcaa tagttattat atatactctg
     1861 cccggactta ttatcacact taccggagca ggggcaggat ttgctggaga attgagcaca
     1921 cactggatac ccaacatagt gttctttatt ttatttgtag tttttgctat atcatttttt
     1981 ggtgcctttg aaataatact gccaaacaga tgggtcagca gtgccgattc gaaggttgac
     2041 agaggtggac tactagcctc attttttctt gcactgacaa cagttatagt atctttctca
     2101 tgtaccggtc caattgcagg aagccttctg gtcgaagcag catcgggcga tattataaga
     2161 cccacaatag gcatgtttgc attcggactg gcattttcaa taccatttac actgtttgca
     2221 cttttccctt cagtaatgag caagctaccc aaatcaggtg cctggcttaa ttcagtgaag
     2281 gttgttcttg cctttctgat gctggcattc agtatgaaat tcctttcaac tatcgacagc
     2341 gtctacggac tcaacattat ttccagagaa atcttcctgg ctgtctggat agttatcttc
     2401 tcactgaccg ggctttacct tatgggtaaa ataaggtttc cgcacgattc ggaagttaaa
     2461 catataagtg ttttcagact ttttctgata attgtggtct tttcgttcgt attatatatg
     2521 ataccgggat tattcggtgc cgaacttaaa ggactatctc cgctcctgcc tgccagaaaa
     2581 tcagtatcat ctttaagccc ggtacaatat caggggtata attctgaatt aaagagtgtt
     2641 actaacactg actcaaacat tggctgttcg cagccaaaat atgccgacag gtttcagctg
     2701 ccgtatggtc tgacaggtta ttttgagtac aaccagggtc ttatgtgtgc aaaggagaag
     2761 gggagtcctg tattaattga ttttaaaggt catgcatgtg caaattgcaa gttgatggaa
     2821 gctaaagtat ggtcggatcc ccgtgtgcag gaacgcctca gaaaatttgt gataattgca
     2881 ttgtatactg atgaccggac acagcttcct gaagaagaat ggattacttc gagtgttgat
     2941 ggaaaagtca aaaagacaat tggtaaaata aatgaagacc ttgagataat gaagtttaaa
     3001 acaaatgcta tccctcttta tgtgattacc gattataacg ggaatcctct tgtaaaacct
     3061 gtggcaacca cgcttaaagt tgaggaatat attgaatggc tcgatgaggg tctgaggcgt
     3121 gcaggagtta attgattaaa ttctgtcaga acatatagga gacagaaatg ccaaggtcgg
     3181 caggcattag cctgaaaaat gaaggtaatc ccacctcaaa aaacggttta atatttaaac
     3241 taacggcaag gggcatatcc ctgaaacggt attcaagggt tccccataca tcgataccta
     3301 taagcggaca tagtctctca gttttgtagt aataatcttt cttttcataa acagtaacat
     3361 attctgtaat aaatccggca tgagctccat aaccccatcc cgtaaaaagg tcgaggataa
     3421 tttcggatag ggcattatca tagaagattt tcatacctgt aagttgaagc ccgttagttc
     3481 ggaatgacag cagggccata acgcctgcat cggcatttcc agaggggctg gcgctctgaa
     3541 aaaatagacc tcctgtgtaa cccgccctga gccctgcctg acgaaaaacc ccctgcccct
     3601 gcagggaata aaaagtgcag ataagcaata aaacctgtat ccaaaccact tttttcatct
     3661 ggaacatgcg tttataaaaa attgatttta ataatgacaa tatttttctt ccggagttgc
     3721 acaattttaa aaattaccgg attaaaatag ttcctctggt ggcatttatt tttaccaccc
     3781 tgctgtcggg atttctcccg acaatgcctg tatgaatgta ttctcttttc tcttcattta
     3841 atacttccct tttaatgtct gcattccctg aagggagtat tacagatgtg ccggtattcc
     3901 ttatttcaag gttatatgat gccgaaggct caaaattgag tgtaaggtcg ttgtaacttg
     3961 aagttacatt tattgttctg aagctatttt cgatcagctc aaaatatgta tctccgtatc
     4021 ttgccgacag atcagtatct gacagcagtc tgtttacacg aagctgggta aagtaggaat
     4081 caccggtcag ggtggttgct gttccaatat aaaatttatc tcttctcgat tcaaccagaa
     4141 gggttttaac ctgttttatg tcatatctcg atgaaatcga tttcaccctg atatggtccg
     4201 attcatcaat aatgaattct gaaaaggaac cgtcgatgtt gcagtcgcct gcgtttttga
     4261 taacaacatt gccaaatgat actttaagct gggagatctt actgagagta tttgctttca
     4321 atgagccatt tgaaagtgaa atatttacat cgttgctgtt ctggtctaca taaatatctc
     4381 cgtatctgtt ttcaattctc agattcatat atggtggagc ctgtatataa taattaatct
     4441 gaacctttga gtcgtaagga ataagttttt ttgtcattcc cttgaaattt tctagaatca
     4501 tgttcaatga ctgctcgaat tctgtttgtg cacgaataaa atattttgtt gaggagattt
     4561 caatgtttat tcctttgatc attttatcag ttctttcctg gtttggtgcc tgggcctcta
     4621 tttctgccct gatagaaaca gaatctttct tccataccgt tacctctacc gttccgtatt
     4681 tattatttat ttcaagggtt gtctcccttg taaccggttc tttccgttca aatattcttt
     4741 tctgagtaaa ctgtgcacct gaatttactg ttgtaccgat gcacagaagt gtcaggagca
     4801 gtttattaaa gacgctggtc tctgtcattt tcattttctt tttcgttttc tttcagtata
     4861 tcaagcattt cctcaagaat cagaaccctg gaacggtaat tttgtatcag agcctctatt
     4921 acttcgctgt ttgcaactct gtctttcagg tcttttttaa tgtcattcat tatactgtca
     4981 agccgttcca tgtcggcata aagttctcta cggagttccg gcctggtatt tagcaggggt
     5041 tctgctgtgc tgaagagctc attgaagcgg acagaataaa aaacaatagc ctcgttaagt
     5101 tctctttcag aagagctgcc tgtaagtggt ttttcaggtt ttgtaataaa gtaaagcatt
     5161 gaaagtccgg ttattattac agcaatagct gcagcggccc gccaataaaa tgtacgaact
     5221 ccggtttcac gtctgatctt tctccacacc gacccatggg gagtgtaaat atcaaattta
     5281 tcacggttct ctttaataaa tttttccagt ttatccatac atcctgtttt ttaaaaggtc
     5341 tttgagtttt gccctggctc ttgaatactg agattttgaa gttgattctg ttattccaag
     5401 tatctgggct atttcgctat ggttgtatcc ttcaagcaga tatagagtaa gaactatcct
     5461 gtacccgtcg ggaagcaatt caatacattt atgcactgct gaaacatcat attcagtttc
     5521 gccttcatca taattgtctt ccatgacttc aggcagggtt tcagggaaaa ccagtgcaag
     5581 cttctttttc ctcagatgat taatgctttt attaatgagt attctcttaa gccatcctcc
     5641 gaaggtcgac tcgaacctga aagagctgag gttcctgaag cactcaataa aagtttcctg
     5701 gaggatgtct tcggcatctt ccctgctgtt tgtcattcga tatgcaaggt tatacatagc
     5761 ctttgaatat tgcctgtaaa gcctgtactg agccctgtgg ttgccttttt tgcattcttc
     5821 aataagaaaa gcaaccgctt cctgctctct atcatcagca tttccggtca taatttctta
     5881 atacaatgac aaattttcaa aggcagggtt gcaaatttag agggtgaatt tcagaaaata
     5941 ttttttataa aattcagagt ttctatttta ttttacaggg tcgaatttaa taaagagact
     6001 ctgtttgtca ccaattttta gccgttcatc tacagttttc tgccattaat gaaaatcctg
     6061 cataaaaaat tgacttttgt ttttttatta tctatattta cataaatcaa aaaccggaat
     6121 acatgaaaag gttaattttt ctcctgatat caattaatct tgttttttct gcagtagctc
     6181 aggtgaaaca ggaagtaatt gccagtgcag gcggatacaa caagagtgca gataacacta
     6241 tttctataag ctggactctt ggagaaacaa tcgtgcctac atatgtttct caggatggct
     6301 cactgatact cacacatggt tttcagcaga agttgattat aacatctatt gaggagaata
     6361 tcgaggtacc ggtaactgtt acagtttatc caaacccgac cagtgagctg ttgaatatca
     6421 agtttgaggc acctgtagat aaactgattc atgtccagat tatggatgca cagggaagaa
     6481 ttgtaaaaaa tgacaggatt gaagtatcgg ttctccagaa aacaatcaac ctgcaggatc
     6541 tgtcaccggg cgtttatttc atgagacttg ttaaaggtac acttatcaat gtgtacaaaa
     6601 ttgttaagct ctaatttttg gttaccagtc agttataatt gcggaaagaa aaatattttt
     6661 caggcagtgt aatataattt taactatcat caagaaaggg gctatctaaa aggcctcttt
     6721 tttgttttca accctgaatt cctgccttta caattgaatt ccggagtcct gcttggatat
     6781 gaaatatctt acggtcaggg ggaaagtaaa ccaagaaata gtaaatagat aaagccccct
     6841 ctttacgaag tagagagggg gtttgggggt gagtacatga ggagaaggag aaaggcgaaa
     6901 gttgagggaa acgaaaccct gaccgcagtg tcaggggtga aaggtgagta catgaataaa
     6961 actgaaaaac aaaatttaaa ggcacaccgg
//
LOCUS       DAOSNB010000118         6965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10894_length_6965_cov_4.375687, whole genome shotgun sequence.
ACCESSION   DAOSNB010000118 DAOSNB010000000
VERSION     DAOSNB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10894_length_6965_cov_4.375687"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLG42_06005"
     CDS             complement(<1..762)
                     /locus_tag="PLG42_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30795.1"
                     /translation="MKNAVRFISAVAVAFMAGLIACDKIPGENQKGTIEFAITVPDEV
                     EGIKSGMKSDSLPAVYNLLVSLEDLTGNHVTDTLIPVYSFGSGFVSEKLELNVGEYKL
                     VKFMVINPAGEVLFASPVAGSSMAYLVKRPLPVIFRVNPNIITRLVPEVLKVENMNPE
                     QFGYVNFGISVVTPVAFWVAALAENPAAVTASLPYTEAKLTVYAADGWSFTFKLTGGV
                     NRLIVRKSEVYKFIAEKEGYLPLKLEITLMQLLQTT"
     gene            complement(906..2612)
                     /locus_tag="PLG42_06010"
     CDS             complement(906..2612)
                     /locus_tag="PLG42_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOU30796.1"
                     /translation="MNEEVLDNSKKESRPGINFIEQIIIEDIRQGKNGGKIVTRFPPE
                     PNGYLHIGHAKAICLDFGLAEKYGGVCNLRFDDTNPEAEDVEYVESIKEDIHWLGFDW
                     GDREYYASDYFDRLYEFAKDLIRRGLAYVDDQPAEVISEQRGSPTRPGIESPYRNRTV
                     EENLDLFERMNRGEFEEGSRVLRAKIDMSSPNMHMRDPVIYRILKTPHHRTGTKWNVY
                     PMYDFAHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQLRTGEYRPRQIEFNRLNLTY
                     TVMSKRKLLELVQGGYVSGWDDPRMPTLCGLRRRGYTPESIRKFIDLIGYTKFEAIND
                     VSLLEHAVRDDLNLHAPRVFGVLNPLKVIITNYPGGVTEEVEVENNPEDPGMGTRKVP
                     FCRELYIDREDFMETPPPKYYRLYPGNEVRLKGAYIIKCTGYRKNDHTGEIDEVYCTY
                     DPETRSGGPSANRKVKTTIHWVSAPHAINAEVRLYDRLFTHEDPLGQKDRDFKEFLNP
                     DSLKILKNCKLEPSLKNAKPLDKFQFQRVGYFCVDPDSTESYLIFNRTVQLKDTWSKL
                     KQ"
     gene            complement(2699..2986)
                     /locus_tag="PLG42_06015"
     CDS             complement(2699..2986)
                     /locus_tag="PLG42_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOU30797.1"
                     /translation="MKYRELDPLLHSQLRLAVISLLAGVEYAEFTYIQEQTGATAGNL
                     SVQLNRLKDAGYIELIKKFRGNYPQTICRITPLGRQKFAEYIDALKDYLKT"
     gene            complement(2967..3563)
                     /locus_tag="PLG42_06020"
     CDS             complement(2967..3563)
                     /locus_tag="PLG42_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30798.1"
                     /translation="MENQEKMMTGEESLHIITEMINRTKGNIRQASFHILFWGWLLFV
                     CSISEYLLHKFTNWATPWYVWFLVIPGIFVSLIYGYVSGRRLRIFTYASMVYMWTWLA
                     FLIAGTVIFIVTSKDPEVFPPLILSLAGMATLISGVIIRFRPLIAGSITFWIFALTAY
                     FGGPDIESLSVSVAMLTGYLIPGYLLKRKENNNEIQRA"
     gene            3747..4127
                     /gene="folB"
                     /locus_tag="PLG42_06025"
     CDS             3747..4127
                     /gene="folB"
                     /locus_tag="PLG42_06025"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOU30799.1"
                     /translation="MGLIQIEGMEFYSFHGHYLEEQIVGSRFIVDLTIETDMSRAALT
                     DNLDDAVNYQRAYEIVRAEMEKKSYLLENIAGRILDALFGELKGIISATVKVSKLNPP
                     VGGKTAAVSVTMSRKNNSQDITKR"
     gene            4179..4763
                     /locus_tag="PLG42_06030"
     CDS             4179..4763
                     /locus_tag="PLG42_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOU30800.1"
                     /translation="MHIDLLEIAAAFMVLFAIIDIPGSIPIILEIKSRTGDVEPLKAT
                     LAASVIFFAFLFIGDPLLGIFGIDVFSFAIAGSFIIFLIGIEMVLGIELFKYHSKGGG
                     SIVPIAFPLIAGAGSITTILSIKAEYYIINIFIALVLNMIIVYLVLRLTSKFEKLLGP
                     SGLLILKKFFGVILLAIAVKLFLTNTGITLPNVR"
     gene            4753..5232
                     /gene="rnhA"
                     /locus_tag="PLG42_06035"
     CDS             4753..5232
                     /gene="rnhA"
                     /locus_tag="PLG42_06035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOU30801.1"
                     /translation="MSGEVHIYTDGAARGNPGPGGYGTILIYGPHRLELSRGYRLTTN
                     NRMELMAVIAGLEALKWPNCNVVVYTDSKYVADAVEKGWVFSWESKQFRKKKNRDLWL
                     KFLELYRKHNVKIVWIKGHNANPENEKCDRLAVEASKGPDLHEDTGYNSEEENILEF"
     gene            5341..5754
                     /locus_tag="PLG42_06040"
     CDS             5341..5754
                     /locus_tag="PLG42_06040"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOU30802.1"
                     /translation="MKGLYIEATKLTPEIRLSPDENLFTIHGHSSPEDVRSLYYPVIE
                     WLEIFINDILEGEYTYSKDNPLKFEFYLPYFNSSSAKFIYDMITELKKLGEKGIPVRI
                     DWFYDIDDPEQKEAGEEMSELAGISFNYFAIKHEE"
     gene            5751..6248
                     /locus_tag="PLG42_06045"
     CDS             5751..6248
                     /locus_tag="PLG42_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOU30803.1"
                     /translation="MMRGQKELYELLDRLSIPFEYHEHPPAATIEEAKAFWKDLNSGK
                     CKNIFFRNHKGNRHYLVILEHLCQLDIHDLEKRLRQGKLTFASDRRLREYLGVEPGAV
                     SPFGLINDKTHHVHVFIDERLGEYERLAFHPNVNDATLIISRDDLIRFITYLGNTYEF
                     IKMYD"
     gene            complement(6260..6937)
                     /locus_tag="PLG42_06050"
                     /pseudo
     CDS             complement(6260..6937)
                     /locus_tag="PLG42_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
BASE COUNT         2008 a         1390 c         1430 g         2137 t
ORIGIN      
        1 agtagtttgc aatagttgca ttaatgtaat ttccaatttc aagggaaggt aaccttcttt
       61 ttccgctatg aatttataga cttcagattt tctgactata agtctgttta ctcctccagt
      121 tagtttgaaa gtaaacgacc agccatctgc agcataaact gtaagttttg cttctgtata
      181 tggtagagaa gcagtaacag cagccggatt ctctgctaaa gcagcaaccc agaaagcaac
      241 aggagttaca acactgatgc cgaaatttac gtatccgaac tgctccgggt tcatattctc
      301 cactttgaga acttcaggca ccagcctggt tataatattg ggatttactc tgaatattac
      361 tggaagaggt cgtttcacaa ggtatgccat ggatgaacct gctaccggtg aagcaaataa
      421 aacttcacca gcagggttga ttaccataaa ttttacaagt ttatattcac caacattgag
      481 ttcaagtttt tcactaacaa aacctgaccc gaaggaataa accggtatca gtgtgtctgt
      541 tacatggttt cctgtgagat cctcaagtga aaccaggagg ttataaacag ctggcaggga
      601 atcggatttc atacccgatt ttattccttc cacctcatcg ggcacagtta ttgcaaattc
      661 aatggtgcct ttctgatttt cacccggtat tttatcacag gcaattaaac ctgccataaa
      721 agcaacggca acagctgaaa tgaatcttac ggcatttttc atggcagtaa tttttagttt
      781 aacattatac aaaattagta tttaatcatc taattatcaa gacaaaaatg ctgccggaaa
      841 ggttgctttt ggatataatt tttctctttc cgggattatt tttaagagtt aaaagcacca
      901 gtgatttatt gttttaattt actccaggta tctttcagtt gaacagtccg gttgaaaata
      961 aggtaagatt ctgttgaatc ggggtcgaca cagaaataac cgactctctg gaattggaat
     1021 ttatcgagag gttttgcgtt tttgagtgat ggttccagtt tgcagttttt aaggatttta
     1081 agtgagtcgg gattcaggaa ttctttaaag tcccggtctt tctggccaag aggatcttcg
     1141 tgggtgaaga ggcggtcgta tagccggact tcagcattaa tagcgtgggg ggccgatacc
     1201 cagtgaattg tggtttttac cttgcggtta gcagatgggc caccactgcg tgtttcagga
     1261 tcgtaagtac aataaacctc gtctatttcg cctgtatggt cattcttccg gtatcctgtg
     1321 cattttatga tgtatgctcc tttaagtctc acttcatttc cggggtagag cctgtaatat
     1381 tttggaggag gagtctccat aaaatcttcc cggtcgatat ataattccct gcagaatgga
     1441 actttccggg ttcccattcc gggatcttca gggttattct ctacttcaac ttcctcagtg
     1501 actcccccgg gatagttggt tataattacc ttcagaggat tcaatacacc gaaaactctt
     1561 ggtgcatgaa ggttaagatc atctctgacg gcatgctcaa gaagagatac gtcgttaatg
     1621 gcttcaaatt ttgtatatcc gatcaggtca ataaattttc tgatggattc aggtgtatat
     1681 ccccggcgtc gcagaccgca gagggtcggc atcctgggat cgtcccaacc tgacacataa
     1741 cctccctgca caagttccag aagcttcctt ttgctcatga cagtataggt gagattgagc
     1801 ctgttgaatt caatttgcct tggtcgatac tcacctgtcc ggagttgatc aataaaccag
     1861 tcgtaaagag ggcgatgtac ttcaaattcg agagtacaga gagaatgggt cactccttcg
     1921 aaatagtcgc attgtccatg agcaaagtca tacatcggat agacattcca ttttgttccg
     1981 gtacggtggt ggggtgtctt aagtattctg taaataacag ggtcgcgcat gtgcatgttg
     2041 ggagaagaca tatctatttt tgctctcaga acacggctgc cttcttcaaa ttctcccctg
     2101 ttcattcttt caaagaggtc gaggttttct tccaccgttc ggttgcggta tggactttca
     2161 atgcccgggc gtgtagggct acccctttgc tcggaaatca cctccgctgg ctggtcgtcg
     2221 acataagcaa gtccccttct gataagatct tttgcaaact cataaagcct gtcaaaataa
     2281 tcagaagcat aatattccct gtcgccccag tcaaatccaa gccagtgtat atcttctttt
     2341 atagattcta catattcgac gtcttctgcc tcggggtttg tgtcgtcaaa ccgcagatta
     2401 caaactccac cgtatttctc ggccaaaccg aaatcgaggc atatagcttt tgcatgacca
     2461 atatgcaggt acccgttcgg ttcaggaggg aaacgcgtca caattttacc cccgtttttg
     2521 ccctgcctga tatcttcaat tattatctgc tcaataaaat ttatacccgg acgtgattct
     2581 ttttttgaat tgtcaagtac ctcctcattc atcgtagatg attttttatg aggtacaaaa
     2641 ataaagaaaa atctgtatca gataattaaa caaaagtaat tgccggtaag ggttatcctc
     2701 atgttttgag gtagtccttc agtgcatcta tatattctgc aaacttttgc ctcccaagtg
     2761 gggttattct gcaaattgtc tgaggataat ttccacggaa ttttttaatt aattcaatat
     2821 aacctgcatc ttttaatcgg tttaactgta cactcagatt tcctgcagtt gcaccagttt
     2881 gctcctgaat atatgtaaac tcagcatatt caacaccagc aagtaaagag atcactgcca
     2941 gcctgagttg tgagtgaaga agaggatcaa gctctctgta tttcattatt attttctttt
     3001 ctttttaaca agtaacccgg gatcaggtat cctgtcagca ttgcaactga aactgacagc
     3061 gattcgatgt caggtccgcc aaaataagct gtaagtgcaa atatccagaa agttatggaa
     3121 ccggcaataa gaggcctgaa tctaattatt actccggata ttaaggttgc catacccgca
     3181 agggaaagaa tgagtggcgg aaagacttca gggtctttcg aagtaacaat gaagattaca
     3241 gtacccgcaa tcaaaaaagc cagccatgtc cacatgtaaa ccatggatgc gtaggtaaat
     3301 attcttaacc tgcgacctga cacatacccg taaattaatg atacgaatat gcccggaatt
     3361 acaagaaacc atacatacca tggagttgcc cagtttgtaa atttgtgcag aagatactct
     3421 gaaatactgc agacgaacaa cagccatccc cagaacagaa tgtgaaaact tgcctgtctg
     3481 atgttacctt tggttctgtt gatcatttca gtgatgatgt gaagactctc ctcacctgtc
     3541 atcatttttt cctgattttc cataataata actacattta tgtgttagtt caaaatgaaa
     3601 ataaacatat tgcaaatata aactagttta aaatataaac caaatttttt ttaaaaaaaa
     3661 tttcagatat tccagaacgt tcataaatca ttttttaaca gattgtggtt ctttttaatt
     3721 tactgccata aataaacatt aagattatgg gattgattca gatagaagga atggaattct
     3781 attcttttca tgggcactat cttgaagagc agatagtagg gagccggttt attgttgatc
     3841 ttacaataga aactgacatg agccgggctg cccttactga taatctggac gatgcagtta
     3901 attatcagcg agcttatgag attgtcaggg cagagatgga aaagaagtca tacttgctcg
     3961 aaaacattgc aggccggata cttgatgctt tatttggaga actaaaagga attatcagtg
     4021 caacggttaa agtatcaaag ctcaaccccc cggttggagg caagactgca gcagtgagtg
     4081 taacgatgtc cagaaaaaac aatagtcagg atataacaaa gagataaaaa gagtcagatt
     4141 ttccttaatc tgtttatctt tgcggaaaaa ttaacaatat gcatattgac ctgcttgaga
     4201 ttgcagctgc attcatggtg ctttttgcca taatcgatat tcccgggtcg ataccgataa
     4261 ttctggaaat aaagtcacgt actggtgatg ttgagcctct taaggcaacc cttgcagctt
     4321 cagtaatatt ttttgctttt ctttttattg gagaccctct tcttggaatt tttggtatag
     4381 atgttttttc atttgcaatt gcaggttcgt tcataatttt tctgataggt attgagatgg
     4441 tactgggaat agagttgttt aaataccatt caaagggagg cgggtcgatt gttcctatag
     4501 catttcctct tattgcaggt gccgggtcaa ttacaacaat actttcaatt aaagctgagt
     4561 attacattat aaatatattc attgcgctgg ttttgaatat gataattgta tatctcgttt
     4621 tgaggcttac ctcaaagttt gagaagctcc tgggtcccag tggacttctg atacttaaaa
     4681 aattcttcgg tgtgatactg cttgcaattg ctgtaaagct ttttttaacc aacactggaa
     4741 taactcttcc aaatgtcagg tgaggttcat atttataccg atggtgctgc aagaggaaac
     4801 ccgggccctg gaggttatgg aacgatcctg atttacggac cccaccgcct tgaattatcc
     4861 agaggctaca ggcttacaac caataaccga atggagctta tggccgtaat tgccggcctt
     4921 gaagcactta agtggcccaa ttgcaatgtg gtagtataca ctgattcaaa atatgttgct
     4981 gatgcggttg aaaaaggatg ggtcttttca tgggagtcaa aacagttcag aaagaaaaag
     5041 aatcgtgacc tctggttaaa atttcttgag ctatacagaa aacataatgt gaagattgta
     5101 tggatcaaag gccataatgc caaccctgaa aatgaaaaat gcgacaggct tgctgttgag
     5161 gcatctaaag gacccgacct gcatgaagat accgggtata attctgaaga agaaaatatt
     5221 ctggaattct gatggaattt gaatgctcag attatgctaa ataataagtt taattcactt
     5281 acgttaaaaa atatattaac tttaaaatgt gaatcatact ggtttcagaa aggtatttga
     5341 atgaaaggtc tatatataga ggctacaaaa cttacacctg aaataaggct ttcgccagat
     5401 gagaatcttt ttaccatcca tggtcattca tcgcccgaag atgtaaggtc gctttattat
     5461 ccagtaattg agtggcttga aatttttata aatgatatac ttgaaggaga gtacacttac
     5521 agcaaggata atcctctgaa atttgaattt tatctgcctt atttcaattc ttcctcggca
     5581 aagttcattt acgatatgat tacagaactg aaaaagcttg gagaaaaggg tattcctgta
     5641 aggattgact ggttctatga tattgatgat ccggaacaga aggaggcagg tgaagagatg
     5701 tcagaacttg cagggataag tttcaattat tttgcaatta aacacgagga atgatgcggg
     5761 ggcagaagga actgtatgaa ttgcttgaca ggctttcaat accttttgaa tatcacgaac
     5821 atcctcctgc agcaactatt gaagaagcga aagctttctg gaaagatctg aattcaggta
     5881 aatgcaagaa cattttcttt cgtaatcaca agggcaaccg gcattatctg gtgattctcg
     5941 agcatctttg tcagcttgat attcatgatc ttgaaaagag gctcagacag gggaaattga
     6001 cttttgcatc cgacaggcgc ctcagggaat accttggtgt tgaacccggg gctgtttctc
     6061 cattcggttt aataaacgat aaaacccatc atgtacatgt gtttatagat gagaggcttg
     6121 gtgaatatga gcgacttgcc tttcatccta atgtaaatga tgccaccctg ataatcagca
     6181 gagatgacct tataagattc atcacctacc tcgggaatac ttatgaattt ataaagatgt
     6241 acgactgatt tttacaggtt tattttccgg cgtttttata aacaggttgt ttccatggct
     6301 caggcggctc atgtttgctc atacatgccc accgtattcc tcttttctgt atttcaagag
     6361 cctgtggaac attgaaatcg gctgccacat gtcccagaga tgaatagaag accctgccgt
     6421 tgccatacat ttttttccat gtaacaggta caactgttcc tccaatccat gaagcatatt
     6481 tgtcggtgaa tgttgttgta gccagtactt tcacattagg atcgacatgc acataatatt
     6541 gttctgaatg catgtcaaag tcggtgagtc cggcggttac ggggtctttt ttgtcgatga
     6601 tattaaccct gtaatcaatt acaccaccag ggtgtgccac ccattgtccg cccaccataa
     6661 actggtattc ggtattatta cggaatgaat cgccaagtcc accgtgccag cctgctatac
     6721 ccaccccgct tttgacagca tctagcaatg ctttttcctg tgctgaggtt atctgcccca
     6781 tggtccatac ctgcactatc agatcaagtt ctttcaaatt catgtttaca tatgcatcaa
     6841 gtgtatcgga aacagtcact ttagcccctt ccgattccat ccatggaaca aatatatccc
     6901 tgcatttgtc gggctcgtgc cccatccatc ctccccagac gaaaagtact ttttaaccct
     6961 gaagg
//
LOCUS       DAOSNB010000119         6962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10906_length_6962_cov_4.252353, whole genome shotgun sequence.
ACCESSION   DAOSNB010000119 DAOSNB010000000
VERSION     DAOSNB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10906_length_6962_cov_4.252353"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLG42_06055"
     CDS             complement(<1..561)
                     /locus_tag="PLG42_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30804.1"
                     /translation="MKTKVILSVIISIAIACIFQARAQSPVVAPPQKKPVMLTGGTIH
                     TGTGEVIENGIIAFSAGKITFVGKASDSRLDRRDYEVIDVTGKHVYPGLILPNTTLGL
                     VEISGVDVTVDSRELGDLNPNVRAIVAYNTDSHVIPVIRSNGTLIAQITPQGTLLPGT
                     ASVVQLDAWNWKDAAYRPDNGLILGWP"
     gene            complement(558..3551)
                     /locus_tag="PLG42_06060"
     CDS             complement(558..3551)
                     /locus_tag="PLG42_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU30805.1"
                     /translation="MRIKLLFLFWLISIAVNSQQDYAPVVGVSDRRPEVYGFRNAKVV
                     VDYQTTMENTDFLVSEGRIISVGKNLSFPPGTVIIDLTGKTVYPSFVDVYAPNYGIRV
                     QQTTSGDASQMARLVAVQQQTGRQAAQQPEPEAHVADYWNEGIKASFNVADEFIPDSR
                     SAGEYRQAGFGAVVTFKADGLARGTSALVTTGDGKANSVMLKNRATANYSFNRGRSSD
                     LYPASLFGIIALLRQFNYDAQWYKQLPPGYLHDEDIEAYLANLSLPQVMEVRNKLEIM
                     RADRIAKEFGINYIIKGAGDEYQALSDVKKTGMKLIIPVNFPEAPDVKDPYDAARVAY
                     TTLKHYELAPSNLSMIASTGIPFTITSADLRQRSSFLANLRKAIKYGLPETEALKALT
                     YTPASLIGAADLVGAIKKNMIANFLITSGNIFSDDCVIYEHWVQGVPYRFVDLRLKDL
                     RGTYDLQVDGEKYKMVLSGTFDKPAVKLLYDTTEIRGATFTLEKDLVSITFERQKIRY
                     RLPGYISGNDLTGKGQLDNGKWVNWKATFVSRDFEPDTRQKRPSPPVIKPGPVPYPFS
                     PYGWIEPPEQEDVLFRNATVWTNEKEGILKNTDVLVKGGKIAAIGRNLSAPGAKVIDA
                     TGMHLTPGIIDEHSHIALDATNEMGQAITSEVRCSDVIDPEDISIYRQLAGGVTAAHI
                     LHGSANPIGGQSVIIKHRWGRPAEELKVEGQVGFLKHALGENVKRTTTRYPNTRMGVE
                     QIIRDAYQRAVDYNREWRTWNALKPSERVGKIPPRRDLELDALVDVLEGRSFIVCHTY
                     VQSEGAMIMNLAQEFGVKVNTLIHFNEGYKIADQIKEHGAAASVFSDWWDYKYEVYEG
                     TTYNAPMLLSQGILTCLHSDDAEMGRRLNQEAAKVIKYGNIPEEEALKLVTLNPAKIL
                     HLDHRMGSIKVGKDADLVLWTDNPLSIYARANKTMIDGVIYFDEDIDARIREQIANDR
                     NRIISNILRESQQPSQSATFNFPRR"
     gene            complement(3659..6493)
                     /locus_tag="PLG42_06065"
     CDS             complement(3659..6493)
                     /locus_tag="PLG42_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOU30806.1"
                     /translation="MPDAFKEFYSSILGRLGKSKAFDDSLRTLAYGTDASLYRLIPRM
                     VIEPACEEEVSLILQKASRYGVAVTFRAAGTSLSGQAVTDSVMVLISDKYWNRYSISE
                     DAGTITVQPALTGGKLNSILARYQRKIGPDPASIDAATIGGIVANNASGLRSRAAGTS
                     YSTVKDLRIIFSDGTILDTGNEQSRKEFLVSHREMVEKILRLSESVKFNEKLAERIRF
                     KYRLKNTTGYTLNALTDFTDPFEIIKHLMIGSEGTLGFISEITLKTVCVKPFRASSLM
                     IFPGVSEACRAVNFLSEMPVEAVEFIDRNGLRSVENEPGMPVFLKKISPEASALLVET
                     TASSKEELDKQIEDVLGKVRNIPSEIPFKFTDVPEEYEELWKVRKGLFPSAGAMRRPG
                     TTVIIEDIAVPVNRLGEAVNDLHRILEKYGYDNTVVYGHALEGNLHFVLSQDFSAEDE
                     KIRYRSLMDEVADLIINKYDGSLKAEHGTGRNMAPFVKKEWGKEAYEVMKKIKEIFDP
                     QNILNPGVILNDDPEIHLRNLKTIPAVDETIDKCIECGFCEPSCVSAGLTLTPRQRVV
                     VYREMEYLRKSGHQPHLLASLAKSFEYHGKTTCAADGLCAINCPVKIDTGKLIKKLRS
                     ERSIMAKKMAEAIAKNLAFVTAFGRLFLKGAYLKHRLFGTRVMKYATMGLRKISLNTL
                     PQWNPYLPGGANKIKTPVRPGKSTRQAVYFPSCINRTMGVSVDHHHEKQLSEKMISLM
                     AKAGYDVIFPEKLNNLCCGMPFLSKGFVEAGMAKSNELENALIRASNNGEYPVVCDMS
                     PCLFTMRENFGDRIRLYEPVEFIMEFLLPYLDITPVNETIAVFPVCSMKKMGLDKKLI
                     ELAGLCAREVIVPETNCCGFAGDKGFFNPELNAYGLRNLKQQIPSTVKHGYSTSRTCE
                     TGLSLHSGISFKSIVYLADMVTRPKNFNSF"
     gene            complement(6512..>6962)
                     /locus_tag="PLG42_06070"
     CDS             complement(6512..>6962)
                     /locus_tag="PLG42_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30807.1"
                     /translation="EEKYKGTPYDTKYIPVTEGRTFNLGGRRIQVMETPGHTPGSICL
                     LDIENKLLFSGDNNNTLVWLFLQNCLPLHKYLETLQKQQKRLNEFTTLLPGHGTPVSS
                     DFINDQAACVKSILDGTCQAKEYKSFAGNAMLCSYGRASVAYRPDNL"
BASE COUNT         1684 a         1568 c         1580 g         2130 t
ORIGIN      
        1 gggccagcca agtatgaggc cattatcagg ccggtatgca gcatctttcc agttccatgc
       61 atcgagctgt acaacacttg ccgtaccggg aagaagtgtt ccctgtgggg ttatctgtgc
      121 aataagggtt ccattggagc gtatcaccgg aataacgtgt gaatcggtat tataggcaac
      181 aattgccctt acgttggggt tcaggtcacc cagctccctg ctgtcgacag taacgtcaac
      241 tccgcttatc tctaccaagc cgagagttgt atttggcaga ataagtccgg gataaacatg
      301 ttttcctgtc acgtcaatca cttcatagtc acgtctgtcg agcctgctgt cagatgcttt
      361 accgacaaat gtaattttac ctgccgaaaa agctataatg ccgttctcaa ttacttcgcc
      421 ggttcctgta tggatggtac caccggtaag cataaccggt tttttctgcg gtggtgccac
      481 aactggactc tgagctcttg cctgaaaaat gcaagctatt gctattgaga ttattacaga
      541 aagtataact tttgttttca tcgtctcgga aaattaaagg ttgctgattg tgatggctgc
      601 tgtgattctc tgagaatgtt tgaaatgatt ctgtttctgt catttgcaat ttgttctctg
      661 atccgggcgt caatatcttc gtcgaaatat attacaccat ctatcatcgt tttatttgcc
      721 cgggcgtata ttgaaagcgg gttatctgtc cacaaaacca ggtcagcgtc tttaccgact
      781 tttatacttc ccatacggtg gtcgagatgg aggattttag ccgggttgag tgttacaagt
      841 ttaagtgctt cttcttcagg tatgtttccg tatttgatta ctttagcggc ttcctgatta
      901 agtcttctac ccatctcggc gtcgtcggaa tggaggcagg taaggatacc ctggctgagg
      961 agcatcgggg cattatatgt tgtgccttca tacacttcgt atttataatc ccaccagtcg
     1021 gagaacacag aagcagctgc cccgtgttcc ttaatctggt ctgctatttt gtagccttca
     1081 ttaaagtgaa taagagtatt cacttttacg ccgaattcct gggcaaggtt cataatcatt
     1141 gcaccttccg actgcacata ggtgtggcac actataaaac ttctgccttc aagcacatcg
     1201 accagtgcat cgagttcaag gtcgcggcgc ggtggtattt tcccaaccct ttctgaaggt
     1261 ttgagagcat tccaggtcct ccattccctg ttatagtcaa cagcccgctg ataggcatca
     1321 cgtataatct gttccacccc catcctggta ttgggatagc gggtagtggt acgcttaaca
     1381 ttttctccga gtgcatgttt caggaagcct acctgtcctt caactttgag ttcttcggca
     1441 ggtctgcccc accggtgttt gattataacc gattgtccac caatgggatt tgcagaaccg
     1501 tgcagaatat gtgcagctgt tacgccgcct gccagttgac ggtagattga aatatcttcg
     1561 ggatcaatta catcgctgca acgtacctct gaagtaattg cctgtcccat ctcatttgtg
     1621 gcatcgaggg cgatatgtga atgttcgtcg attattcccg gtgtaagatg cattcctgtt
     1681 gcgtctatga cttttgctcc gggagcagaa aggttcctgc caattgcagc aattttgcct
     1741 cccttaacaa gaacatccgt atttttaagt atgccttcct tttcattcgt ccacactgtt
     1801 gcattgcgga acagaacatc ctcctgctct ggaggttcta tccatccata gggagaaaac
     1861 ggataaggaa caggtccggg ttttatcaca ggtggtgagg gccttttctg tctggtatca
     1921 ggttcaaaat ccctgcttac gaatgttgcc ttccagttga cccattttcc gttgtcgagc
     1981 tgaccctttc cggtcaggtc gtttccggat atgtatcctg gcagcctgta acgtattttc
     2041 tgtcgttcaa aggttatgct aacaaggtct ttttcaagcg taaaagttgc tccccttatt
     2101 tcagtagtgt catatagaag cttaactgca ggtttgtcaa atgtccctga aagaaccatt
     2161 ttatattttt ctccatcgac ctgcaggtcg taagttcctc tcaggtcctt tagccggagg
     2221 tcaacaaacc tgtaagggac accctgcacc cagtgctcgt agattacgca gtcgtcgctg
     2281 aaaatgttgc ctgatgtaat caggaagttt gcaatcatat ttttctttat agcacccaca
     2341 aggtcggctg ctccgattaa tgaagccggg gtatatgtca gagctttgag agcctctgtt
     2401 tcaggtaatc cgtatttaat tgcctttctg aggtttgcaa gaaatgatga gcgctggcgg
     2461 aggtctgccg atgtaatggt gaagggtata cctgttgaag caatcataga aaggttcgac
     2521 ggagcaagtt cgtagtgttt aagggttgtg taagccaccc ttgccgcatc ataaggatcc
     2581 tttacatcgg gggcttcggg gaagttgaca ggtataataa gtttcatacc tgttttcttt
     2641 acatcgctga gtgcctggta ttcgtcgcct gcacctttaa ttatatagtt aatgccgaat
     2701 tcttttgcta tcctgtctgc ccgcattatt tcaagtttat ttcttacctc catcacctgt
     2761 ggcaacgaaa ggtttgcaag gtaagcttcg atatcctcat cgtgcaggta gccgggtgga
     2821 agttgtttat accactgggc gtcgtaatta aactgtcgca ggagggctat aataccgaag
     2881 agggaagccg ggtagaggtc ggacgagcgt ccccggttaa atgagtagtt ggctgttgca
     2941 cggtttttga gcatcacact atttgcttta ccgtcgcccg ttgtcacaag ggctgatgtt
     3001 cctctagcaa ggccatcggc tttgaaagtt acaacggcgc cgaacccggc ctgccggtat
     3061 tctcctgcag agcgtgagtc gggtataaac tcatctgcaa cgttaaatga agctttgatg
     3121 ccttcattcc agtaatcagc cacatgggct tcaggttcag gctgctgtgc cgcctgcctt
     3181 ccggtttgct gttgaaccgc aaccaacctt gccatctgtg aggcatcacc agaggttgtc
     3241 tgctgtaccc tgatgccata gttgggagca tacacatcca caaaggatgg atagacagtt
     3301 tttcccgtaa ggtcgattat tactgtgccg ggaggaaatg aaaggttttt acctacggaa
     3361 attatccttc cctccgacac aaggaaatca gtattttcca tagtagtctg ataatcaacc
     3421 actacttttg catttctgaa tccgtaaact tccggccttc tgtcgcttac ccctacaaca
     3481 ggagcataat cctgctggga attgactgca atagatataa gccagaacag gaagagcagt
     3541 ttaattcgca taacaattca attttaaaga gatctcaggt ttgtttttaa aaatattaag
     3601 atcaggttac tcagggctaa agatataaac atagatgttc ggttattcat ttacagagtt
     3661 aaaaagaatt aaagtttttt ggccgggtaa ccatgtcggc aagataaact attgatttaa
     3721 atgagatacc cgaatgaaga gaaaggcctg tttcgcatgt tctgctggtt gaatatccgt
     3781 gtttcacggt tgatggtatc tgctgtttga ggtttctgag tccgtaagca ttgagttcag
     3841 gattaaagaa tcctttgtca ccggcaaatc cgcagcagtt tgtctccggt acaatcactt
     3901 ctctggcgca taaaccggca agttcgataa gtttcttatc cagacccatt ttcttcatgc
     3961 tgcataccgg aaatactgca atagtttcat tgacaggcgt tatatcaagg taaggcagaa
     4021 ggaactccat aataaattcc acaggttcat aaagtctgat tctgtcgccg aaattttctc
     4081 tcatggtaaa aaggcacggg ctcatatcgc atacaacagg gtattcacca ttattgcttg
     4141 ccctgataag tgcgttttca agctcatttg attttgccat tccggcttcg acaaatccct
     4201 tactcagaaa cggcatgcca caacaaaggt tattcagttt ttcagggaag ataacatcat
     4261 agcctgcttt tgccatcagc gaaatcattt tctccgaaag ttgtttttca tggtggtggt
     4321 caacagatac gcccattgtg cggtttatac aagaagggaa gtatacagcc tgccgtgtac
     4381 ttttacccgg acgaacagga gtcttgattt tatttgcccc gccgggaaga taaggattcc
     4441 attgaggaag agtatttaac gatatttttc tcagccccat ggtagcgtat ttcatcactc
     4501 tggttccaaa tagcctgtgt ttaaggtaag ctcctttgag aaagagtctg ccgaatgctg
     4561 taacaaatgc aagatttttt gcgatggctt ctgccatttt ttttgccatg atggatcttt
     4621 cagagcgcag ttttttaatg agttttccgg tgtctatttt tacagggcag tttattgcac
     4681 acaggccatc ggcggcgcat gttgttttac catgatattc aaatgatttc gccagggatg
     4741 ccaacagatg gggctggtgt ccgctctttc tgaggtattc catttctctg tatacaacta
     4801 ctctctgccg cggggtaagc gtaagtccgg ccgatacgca tgagggttca cagaagccac
     4861 actctatgca tttatcaatt gtttcgtcaa cagcaggaat ggttttaaga ttcctgaggt
     4921 ggatttccgg gtcatcgttg agaataactc ctgggttaag gatattctga ggatcgaaaa
     4981 tctccttaat cttcttcatc acctcatatg cttctttgcc ccactctttt ttcacaaacg
     5041 gagccatatt ccgtccggtt ccatgttctg ccttcagtga cccgtcgtac ttattaataa
     5101 ttaaatctgc cacttcgtcc atcagactgc ggtaccgtat cttttcatcc tctgcggaga
     5161 aatcctgact gagtacaaaa tgaaggtttc cttccagagc gtggccgtaa acaacagtgt
     5221 tatcgtaacc atatttttcc agaattcggt gaaggtcatt aacagcttca ccgagcctgt
     5281 ttactggcac cgcaatatct tcgatgataa cggttgtacc tggtcttctc atagcgcctg
     5341 ccgacgggaa gagacctttc cgtactttcc agagttcctc atattcttca ggtacatcag
     5401 taaatttaaa tggtatctct gacgggatat ttcttacttt tccgagtaca tcttcgattt
     5461 gtttatccag ctcctctttt gatgaggctg ttgtttcaac cagcagggct gatgcttcag
     5521 ggctaatttt tttaaggaag acgggcattc ctggttcatt ctccaccgac cggaggccgt
     5581 tacggtcgat aaattcaact gcttccacag gcatttcaga aagaaaatta actgccctgc
     5641 aagcttcaga aacccctgga aatatcatta gtgaactggc tctgaatggt ttaacacaaa
     5701 ctgttttgag agtaatttca gagataaagc ccagagtacc ctcggagcca atcatcaggt
     5761 gttttattat ttcgaatggg tcagtgaaat cagttaaagc gttaagtgta tatccggtag
     5821 tatttttaag tctgtattta aatctgatcc gttcagcaag tttctcattg aatttcaccg
     5881 attcagaaag ccgaagaatt ttctcgacca tttcccggtg agaaacaaga aattctttcc
     5941 tgctctgttc attgcctgtg tcaagtattg tgccgtcgga aaatatgatc ctgaggtctt
     6001 ttacggtgct gtagcttgta cctgctgccc tgcttctgag tccgcttgcg ttgtttgcca
     6061 ctattccgcc aattgttgca gcgtcaatag atgctgggtc agggcctatt ttccgctggt
     6121 acctggcaag aattgagttt aattttcctc cggtgagtgc tggctggacg gttattgttc
     6181 ccgcatcttc tgaaatgcta tatctgttcc agtatttatc ggaaatcaga accattactg
     6241 aatcagtaac tgcctgtccc gagaggcttg tacctgcagc cctgaaggtt actgccactc
     6301 cgtagcgtga tgctttctga agaattaaag agacttcttc ttcgcaagct ggttcaataa
     6361 ccattcgagg aatcagtctg tagaggctgg catctgttcc ataggcaagt gtgcgcaggg
     6421 aatcgtcgaa agcttttgat tttccaagcc ttccaagtat tgatgagtaa aattccttaa
     6481 aagcatcagg cataaatgca aataagaatc tttaaagatt atcaggtctg taggcgacgc
     6541 tggcacggcc atatgagcaa agcatggcgt taccggcgaa tgacttatac tcttttgcct
     6601 ggcaggtgcc gtcgagaatg cttttaacac atgcagcctg atcgttgatg aaatcggaag
     6661 agaccggagt gccgtgaccc ggcaatagtg ttgtgaattc attcagccgt ttctgctgtt
     6721 tctgaagcgt ctcgagatat ttgtgcagcg gcaggcagtt ctgaaggaaa agccatacca
     6781 gcgtattgtt attgtcgccg ctgaaaagca gtttgttttc aatatcgagc aggcatatgc
     6841 ttccaggagt gtgtccaggt gtttccatta cctgtatgcg ccggcctcca aggttgaaag
     6901 tgcgtccttc cgtcacagga atgtattttg tgtcgtaagg ggtccctttg tatttctcct
     6961 ct
//
LOCUS       DAOSNB010000120         6960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10913_length_6960_cov_4.151050, whole genome shotgun sequence.
ACCESSION   DAOSNB010000120 DAOSNB010000000
VERSION     DAOSNB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10913_length_6960_cov_4.151050"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PLG42_06075"
     CDS             complement(<1..1013)
                     /locus_tag="PLG42_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU30808.1"
                     /translation="MLYYLFKYLDSLDVPGAGVFEYISFRSAAAVITSLIISLLIGKR
                     IIQYLQVKQIGEVVRDLGLEGQYRKEGTPSMGGIIILASIVIPVVLFAKLNNIYIILM
                     LTTTMWLGFMGFLDDYIKIFRKNKAGLAGKFKIAGQVGLGLIVGVVFYFSDDVIIVER
                     VPVSSLTRAERAEIIENNRNVLQQSEINIERKSTKTTIPFVKNNEFDYRWLVSFAPEP
                     WREILTWIVYVIIVIFIVTAVSNGANLTDGLDGLATGVSAITGAALGALAYVSSNIIY
                     ARYLNIMYIPGSEELVIFASAFIGATVGFLWYNSYPAQVFMGDTGSLALGGIIAVFAI
                     VIR"
     gene            complement(1025..2482)
                     /locus_tag="PLG42_06080"
     CDS             complement(1025..2482)
                     /locus_tag="PLG42_06080"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOU30809.1"
                     /translation="MKLEEILKGIEVSEYEGDMSVTVTDVTIDSRSVVPGSLFVALKG
                     TKTDGHRFIDAAVSSGAAAIICSEMPTVRQKGVTWIRVPDTAPAAGIASSNFFGNPSH
                     HITLTGVTGTNGKTTIATLLYRLFSGLGYRCGLFSTVCNYISGKEYPATHTTPDPVQL
                     NRLMAEMVREGCSHVFMEVSSHAVDQKRISGLKYAGGIFTNLTHDHLDYHKTFDNYLK
                     AKKAFFDSLQANAFALVNTDDKNAGVMVQNCRARKYTYSTRGMADFRAVLIEQSMEGM
                     GIRIDGEELWTRFIGEFNVSNLLAVYASAVLLGAERKEVLRVLSGLGPVPGRLEVVRG
                     NAPVTGIVDYAHTPDALVNVIETINKVRRGSGRLITVVGAGGDRDRTKRPIMASICAR
                     GSDRVILTSDNPRSEDPDTIIDEMEAGITPDLRPKVLRITDRREAIKAAVMMASNGDV
                     ILVAGKGHETYQEIKGIRHHFDDREELAKALMGKM"
     gene            complement(2496..4610)
                     /locus_tag="PLG42_06085"
     CDS             complement(2496..4610)
                     /locus_tag="PLG42_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOU30810.1"
                     /translation="MAIRDEIIWRSGVIYVVTSIIAIVLLVRIVLFQFFERSKWEALG
                     EKYVYKNDEVKATRGNILACDGRLLVSSVPYYSVYMDTRSTGMPPELWNENINELCRG
                     LSEILGEYSPAGWKNILTSARKRGERYFLIRKGVNYETLKQLKELPIFREGQYRGGFV
                     AQPENKRIFLNSELAARTIGYLTDDPEGVKVGIEGAFDRELAGRNGKVLKQRLTGGVW
                     IPVNDGNNVEPVDGNDVVTTIDIELQDVATTALRNQLRKHNADHGCAILMEVATGDIK
                     AIANLKRGENGEFYEAYNYAIGESTEPGSTFKLPVLMAALEDGVIDTSDVVDTGNGTV
                     RFYNKIIRDTREGGYGKLTVKEVFEKSSNVGTSKIIYQHYKDNPRALISRLNSMKLNE
                     KLNLQIKGEAEPYIRQPGDKLWSGLSLPMISHGYEVRFTPLQILTFYNAVANDGCMMR
                     PRFVKEIRRNGNVIKRFDPEVITNSIASMKTIRKAKKMLEGVVEHGTATNLRDADYKI
                     AGKTGTAQIARNNTGYRSGERIYYQASFVGYFPAEDPLYSCIVVVNSPSSSVYYGNMV
                     AGPVFKEIADKVYSTIFFKDYPEKVNDDKIIATPDPGNGFYDDIHSILRKTDAIYFKN
                     GNEKWVATGRSRDTIRIVPLKIQENLVPDVRDMTLRDALYLLESYGLRVQFSGRGRVK
                     RQDPGYGAVIHDGMVISLDLGI"
     gene            complement(4625..4984)
                     /locus_tag="PLG42_06090"
     CDS             complement(4625..4984)
                     /locus_tag="PLG42_06090"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOU30811.1"
                     /translation="MEEKAQDNMEGKKKKNSGSFLKDLLSGSTISDKIILKNLRFLIF
                     LTFLGALYIANRFHAEKLVREMSQLQKEVRELRAESLATSAELMKATRQSEILRQVTQ
                     RGLELEELREPPYKLVK"
     gene            complement(4993..5892)
                     /gene="rsmH"
                     /locus_tag="PLG42_06095"
     CDS             complement(4993..5892)
                     /gene="rsmH"
                     /locus_tag="PLG42_06095"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOU30812.1"
                     /translation="MAYHVPALLDESIKGLNIRPDGVYVDATFGSGGHSREILKHLTT
                     GRLIAFDIDEDASANIIEDERFTFLNQNYRFLLNNLRYLGITEVDGIIADLGVSFHQF
                     DEPSRGFAFRYDAELDMRMSRGMKTKASDLLATMTEEELAHIFTEYGELPLARKIARA
                     IAVAREKAPLKTAEDLKKVTAHFVPPGHQNRFFAKLFQALRIAVNNELESLKEFLLQS
                     AEILKKGGRLVVITYHSLEDRLVKNFMKSGNFTGEVSRDFYGRPELIFRLVTKKAVTP
                     GRAETETNKRARSAKLRVAEKIK"
     gene            complement(5893..6354)
                     /locus_tag="PLG42_06100"
     CDS             complement(5893..6354)
                     /locus_tag="PLG42_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOU30813.1"
                     /translation="MATFIGDYGCRLDAKGRFILPAAFKKQMPPGSPDRFVVRRDIFE
                     PCLVLYTIDDWNIQLEKIRRRINPYNREHNVFLRNFFKGTAELALDSSNRLLIPKRLL
                     ELAGIGRDIVLAGQDGRIEIWDAERYEKIEMLPEDFANLAEKLLGNSQNQE"
     gene            6629..>6960
                     /locus_tag="PLG42_06105"
     CDS             6629..>6960
                     /locus_tag="PLG42_06105"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOU30814.1"
                     /translation="MDDPYASGNESYRLDVKEVLRAKNPSLARIVPKFIVNYLKKIVH
                     ETELNDFLGKYGHLKGAEFIHAALNYLKVSYTVSGREHIPTRGRFIFVSNHPLGGLDG
                     LVFIYELS"
BASE COUNT         1819 a         1691 c         1290 g         2160 t
ORIGIN      
        1 tttctgataa ctattgcaaa cactgctata atgcctccca gtgcaaggct acctgtatcg
       61 cccataaaca cctgagcggg ataagaattg taccacagga atcctacagt agcacctatg
      121 aaagcacttg cgaagatcac cagttcttca gaaccgggta tatacattat attaaggtaa
      181 cgggcataaa ttatgttact cgacacataa gccagtgcac caagcgctgc cccggtaatt
      241 gctgatacac ctgtcgcgag accatcaagc ccgtcagtca ggtttgcacc attcgatact
      301 gcggtaacga tgaatattac aatgatcaca taaacaatcc atgtgagtat ctcccgccat
      361 ggttccgggg caaaagaaac cagccacctg taatcaaact cattgttctt tacgaacggt
      421 atagtggttt ttgtggattt tctttcaata tttatttcgc tttgctgcag aacattcctg
      481 ttattctcaa taatttcagc cctttctgcc cttgtaagac tcgataccgg aaccctttca
      541 acaatgataa catcgtcact gaaataaaat accacaccga caataagacc aaggcccacc
      601 tgtcctgcta tcttgaactt tcctgcaagt ccggctttgt tttttctgaa tatctttata
      661 taatcgtcaa gaaatcccat aaagccgagc cacattgtag tggtcagcat aaggattata
      721 tagatattat tgagctttgc gaaaaggact acaggtataa caattgaagc aagtattatt
      781 atacctccca tagaaggtgt tccctccttg cggtattgtc cttccagtcc gagatccctc
      841 acaacctcac ctatttgctt tacctgcagg tactgaatta ttctttttcc gattagaagt
      901 gatataatca gggaagtaat tactgctgca gccgatctga aagagatata ttcaaatact
      961 ccggcacctg gtacatcaag cgaatcaaga tatttaaaaa ggtaatagag cattttttcg
     1021 ttttttacat tttacccata agcgctttag caagttcttc cctgtcgtcg aaatggtgtc
     1081 tgataccttt tatttcctgg tatgtttcat ggcctttacc ggcgacaaga attacatcac
     1141 cgttgctggc catcatcact gcagctttta tagcttctct gcggtcagta attctcagca
     1201 cttttggcct caggtcgggt gtgatgcctg cttccatctc atcaataata gtatcggggt
     1261 cttcacttct tggattatcc gatgtaagaa tgactctgtc gcttcctctt gcacatattg
     1321 aagccattat tggtcttttt gtccggtccc tgtcgcctcc ggctccaacc acagtaataa
     1381 gtctaccgct gcctctgcgc actttgttta ttgtttcaat cacattcaca agagcatctg
     1441 gagtatgagc ataatcaact attccggtta caggggcatt tcccctcacc acttcaagcc
     1501 tcccaggtac cggtcccagc ccgctaagca ccctgagcac ctctttcctt tctgcaccca
     1561 gcaaaacagc cgacgcataa acagcaagaa ggtttgaaac attaaactcc ccaataaacc
     1621 tcgtccataa ttcctcaccg tcaatgcgta ttcccatccc ctccatgctc tgttcaataa
     1681 gcacagccct gaagtcagcc ataccccttg ttgaataggt atattttctt gccctgcaat
     1741 tctgaaccat cactcctgca tttttatcgt cggtattgac aagagcaaat gcattggcct
     1801 gaagagaatc aaagaatgcc ttttttgctt tcaggtagtt atcaaaggtt ttgtgataat
     1861 caaggtggtc gtgggtgagg tttgtgaata tgcctcctgc atatttaagt ccgctgattc
     1921 ttttctgatc aacggcgtgt gagctgactt ccatgaacac atgagaacat ccttcacgaa
     1981 ccatctctgc cataagcctg ttcaattgaa cagggtcagg tgtagtatgc gtggcaggat
     2041 attcctttcc ggaaatatag ttacataccg tcgaaaaaag tccgcacctg tatccaagac
     2101 cactgaaaag tctgtataaa agtgttgcaa ttgttgtttt tccatttgta cctgtaactc
     2161 cggtaagtgt aatgtggtgt gaaggatttc cgaaaaagtt tgatgaggca attcccgctg
     2221 caggtgctgt atcgggtact cttatccatg taacgccttt ctgtctgacg gttggcattt
     2281 cagaacagat aattgcagct gctccagatg agacagccgc gtcaataaac ctgtgtccgt
     2341 cggttttggt gcctttcaga gccacaaaaa gagaaccggg aacaacagaa cgagaatcaa
     2401 tagtgacatc tgttacagtc actgacatgt ctccttcata ctctgatacc tcgattcctt
     2461 ttaatatttc ttccagtttc atcatcatta tatttctaga tacccagatc caatgaaata
     2521 accattccgt cgtgaattac agcgccgtat ccagggtcct gccttttcac ccgccccctg
     2581 ccagaaaact gcaccctcag tccgtaactt tcaagcagat aaagagcatc cctcagtgtc
     2641 atatccctta catccggcac aagattctcc tgtattttca atggtactat cctgatagta
     2701 tcccggctac gtccggtagc aacccatttt tcattaccat ttttaaaata tatcgcatca
     2761 gtctttctta agatgctgtg tatatcgtcg taaaatccat tacccggatc gggagttgct
     2821 attattttat catcattgac cttctcagga taatctttga aaaaaattgt cgaataaacc
     2881 ttatcggcta tttctttaaa aacaggacca gctaccatat tcccgtaata aactgagctg
     2941 gaaggtgagt taaccacaac gatgcatgaa tatagagggt cttcagcagg gaaatatcca
     3001 acaaaagaag cctggtaata tatgcgttct cctgacctgt atccagtatt atttctggca
     3061 atctgggcgg taccggtttt tcctgcaatc ttataatcag catccctgag gttggttgcg
     3121 gttccatgct ccaccacacc ctcaagcatt ttctttgctt tacggattgt tttcattgaa
     3181 gcgatagagt ttgttatcac ctcaggatca aatctcttta tcacattccc gtttcttctt
     3241 atctccttaa caaatctcgg acgcatcatg cagccatcgt ttgccacggc attgtaaaaa
     3301 gtcagtatct gtaacggtgt aaatctgacc tcataaccat gggatatcat tggaagtgaa
     3361 agacccgacc agagtttatc ccccggttgc cttatatatg gttctgcctc accttttatc
     3421 tgcagattaa gtttttcgtt aagcttcatt gaattgagcc ggctgattaa agctcgcggg
     3481 ttatccttgt aatgctggta tataattttt gatgtcccta cattggatga cttttcaaac
     3541 acttctttta cggtcagttt tccataacca ccctccctgg tgtctctgat tattttatta
     3601 taaaacctga ctgttccgtt accggtatcc accacatcac ttgtgtcgat caccccgtct
     3661 tcaagtgcag ccatcagtac cggcagcttg aatgtagagc ccggttcagt gctctcacct
     3721 atagcatagt tgtaagcttc gtagaattca ccgttttctc cccgcttcag gttagctatg
     3781 gctttgatat ctcctgttgc aacttccatc agaatggcgc atccatggtc ggcgttatgt
     3841 tttcttagct gatttctgag tgccgtagtt gcaacatcct gaagctcaat atctattgtt
     3901 gtaacaacat cgttaccatc aacaggttct acattatttc cgtcatttac tggaatccat
     3961 actccgccgg ttagcctctg tttaaggact ttcccatttc ggcctgcaag ttccctgtcg
     4021 aaagcacctt ctatccccac ttttacacct tcagggtcat cggtaagata tcctattgta
     4081 cgggcagcca gttcgctatt caggaagata cgtttgttct caggctgtgc cacaaaccct
     4141 ccgcgatact gtccttcccg gaaaataggc agttccttta gctgcttcag agtttcataa
     4201 tttactcctt tcctgatcag aaaatatctt tccccccttt tccgggccga ggtaagtata
     4261 tttttccagc cagctggtga atattctccc aggatttcgg ataatcccct gcacaattca
     4321 ttaatatttt cattccacag ttcaggaggc atcccggtac ttcttgtatc catgtaaacc
     4381 gaataatatg gcacagagct gaccagaagg cggccatcac atgcaagtat gttgccccgt
     4441 gttgccttaa cctcgtcatt cttatataca tatttctcac ccagcgcctc ccatttactc
     4501 ctctcaaaaa actgaaaaag gacaatcctc accagcaata ctattgcaat gatgctcgta
     4561 acaacataaa ttaccccgct cctccatatt atttcatccc tgattgccat ccctgttctt
     4621 tttattactt aacaagctta taaggtggtt cacggagttc ctcaagctca agccctctct
     4681 gggtgacctg gcgaagtatt tccgactgcc tggttgcctt catcagttcg gctgaagtgg
     4741 caagggattc tgctctaagt tccctgacct ctttctggag ctggctcatt tcccttacca
     4801 gtttttcagc atgaaaacgg tttgctatat ataatgcacc cagaaatgtc agaaatatca
     4861 gaaatctaag atttttcaat attattttgt cagatatcgt gctgccgctg agtaaatcct
     4921 tcagaaaact tcccgaattc tttttcttct ttccttccat gttatcctgt gctttttctt
     4981 ccataaagaa tcctatttta ttttttcagc cactctcagt ttggcacttc ttgccctctt
     5041 atttgtttct gtttcagccc ttccaggtgt aacagctttt tttgtaacca gccggaaaat
     5101 gagctccggc ctgccgtaga aatctcttga tacttcacct gtgaaatttc cagacttcat
     5161 aaaattctta accagcctgt cttcaagcga atgataggtg ataacaacaa gtctgcctcc
     5221 tttcttgaga atctcagcag attgcagaag aaactcctta agtgattcca gttcgttgtt
     5281 gactgctatc cgtagtgcct gaaagagttt agcaaaaaat ctgttctggt gacctggtgg
     5341 tacaaagtga gcagtcactt ttttcagatc ttcggccgtc tttaaaggag ctttttctct
     5401 tgccacagca attgccctgg caatctttcg tgccagaggt aattctccgt attcggtaaa
     5461 tatatgggca agttcttctt cggtcattgt agcaaggagg tccgatgctt ttgttttcat
     5521 tcccctgctc attctcatat cgagctctgc atcataacga aaagcaaaac cacgtgaggg
     5581 ttcatcgaac tggtgaaaag agacacccag atctgctatg ataccgtcaa cctctgttat
     5641 tccaaggtat ctcaggttgt taagaaggaa tctgtaattc tggttaagaa atgtaaaacg
     5701 ttcgtcttca atgatatttg cgcttgcatc ctcatcaata tcgaaagcaa tgagacgccc
     5761 tgttgtaaga tgtttaagta tttcacgtga atggcccccg ctgccgaaag tagcatcaac
     5821 atatacaccg tcgggccgga tattcagccc ctttatactt tcatccagca atgcaggtac
     5881 atggtaagcc atctattcct gattttgact gttaccaaga agtttttctg ccagatttgc
     5941 aaaatcttcg ggaagcattt caattttttc atagcgttct gcatcccaga tttcaatgcg
     6001 gccatcctgc cctgccagaa caatatcccg tcctatgcct gccagctcaa gtaaacgctt
     6061 tggtatcaac aggcggttac tgctgtcaag agccagttca gctgtccctt taaaaaagtt
     6121 cctgaggaaa acattgtgtt ccctgttgta aggatttatt cttctcctga ttttttcgag
     6181 ctggatattc cagtcatcaa tggtatagag aacaagacat ggttcaaaaa tgtctctgcg
     6241 aaccacaaac cgatcaggtg aaccaggagg catctgcttc ttgaatgccg caggaaggat
     6301 aaacctgccc tttgcatcaa gcctgcaacc gtaatcacct atgaatgtgg ccataatagt
     6361 tgaataaatt tcaaattgct aaaataagta aaaatttcca cttttctcca ctttacccca
     6421 tttttttcca ctattgttga taactttttt cagcattctc cggtattcta aagtttcctt
     6481 gctatgactt tggatattaa cacattcaga taaagctctt taacaagcat ctgcaaaagc
     6541 cagaatattg gatggcagtt tcaaaacctt gcactggaga tgatttttta ttaaatttgg
     6601 gtctggttta aaattttgac aacgacatat ggatgatcct tatgcgagcg gtaatgaatc
     6661 ttacagactc gatgtcaaag aagtgctgag agctaaaaat ccgtcactgg cccgtattgt
     6721 tccaaaattt attgttaact accttaagaa aatagtacac gaaaccgaac ttaacgactt
     6781 tctggggaag tacgggcatt taaaaggtgc tgagtttatc catgctgcac ttaattacct
     6841 caaagtaagc tatacagttt cgggacggga gcatatcccc acccgaggca gatttatttt
     6901 cgtttcgaac cacccactcg gaggtctcga cgggctggtt tttatctatg aactgtcgaa
//
LOCUS       DAOSNB010000121         6948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10942_length_6948_cov_5.044103, whole genome shotgun sequence.
ACCESSION   DAOSNB010000121 DAOSNB010000000
VERSION     DAOSNB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_6948_cov_5.044103"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1560)
                     /locus_tag="PLG42_06110"
     CDS             complement(19..1560)
                     /locus_tag="PLG42_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOU30815.1"
                     /translation="MKEENKKYRKYLIWFWGIIALPYIFIIVMFLLISAEKLGPMPTF
                     EELENPENNLAAEVYSEDGVLLGKFYIENRTWTDYKDISKNVINALLATEDIRFYRHS
                     GIDIRGLGRVFIRSILLQQEAGGGSTISQQLAKNLFKTRGGVDSIRYSRVKRKVNLVI
                     SKFKEWYTAVKLEKNYTKEEIIAMYLNVFDFVNNAVGIRSAARIYFNTTPDSLTIEQS
                     AMLIGMLKNSSRYNPLKRPELTLARRNVVLDQMAKYQYITYAEADSLKKLPLGLNYTE
                     ESHNTGLATYLREYIRTTMIKFEPDRRFFSYEEQYNDAVWQWENNPLYGWCRKNKKPD
                     GSNYNLYKDGLKIYTTINSRMQKYAEEALTEHLKEIQPAFDRLAKTFKNPPFSNDLTK
                     KQVEEILMSAIRRTDRYREMVRRKVPEDSIMLAFNTPVKMRVFSWKGERDTVMTPLDS
                     IRYYKHFMRSSFMIMDPHTGHIKAYVGGPDFRYFKYDAVTEQKRQVGSTIKPFLYTLA
                     IQNGF"
     gene            1698..4004
                     /gene="topA"
                     /locus_tag="PLG42_06115"
     CDS             1698..4004
                     /gene="topA"
                     /locus_tag="PLG42_06115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOU30816.1"
                     /translation="MIENLVIVESPAKAKTIEKFLGKDFRVVSSFGHIRDLTKKNLGI
                     DIKGGFIPDYEIPKDKIKVVNELRKAASEAKTIWIASDEDREGEAIAWHLVEVLNLDP
                     QKTRRIVFHEITKSAIEEAIKSPRTVDMNLVNAQQARRILDRLVGFEISPVLWRKVQP
                     ALSAGRVQSVAVRLIVEREREIINFKPESSFRITGIFNKDKSSDNLLVEEPPLKAEAI
                     TKFHDEDEAVKFLELCREAVFTVKNVIVKPGTRSPAPPFITSTLQQEAYRKLGFSVSQ
                     TMAIAQKLYEEGKITYMRTDSTNLSRMALAKIREVIVNEYGEKYSKVRQYKTTSKGAQ
                     EAHEAIRPTYPEKSTISGTQAEKRLYELIWKRTIASQMADAQTEKTNIAIEMNNSPVL
                     FNATGEVILFDGFLKVYSESQDTEGGEDEKAILPSVKPGMLLFYDRITAIEKYTTPPP
                     RYTEASLVKKLEELGIGRPSTYAPIISTIQQRGYVARENRPGEKRKITVLELSAGNIT
                     KSTKTEIFGKEKSKLFPKDIGMIVNDFLMENFSEIVDYNFTAEVEEQFDKIAAGKLAW
                     NKMLENFYGPFHKTVENTLEKKNKTTGTRILGTHPETGETISVKMGKYGPVVQAVHPE
                     KGDKPRFASLQKNQLMETITLEEAMKLFSLPRSLGNWEGDEVVVGTGRFGPYIRYRNK
                     FYSLKNIDDPYLITLERAIEIIKEKDEADKQKVIKEFGEIKIINGRYGAYINYRGENY
                     RIPKGTNAEDLTQEECMNIIEKKQKSGK"
     gene            4001..5173
                     /locus_tag="PLG42_06120"
     CDS             4001..5173
                     /locus_tag="PLG42_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOU30817.1"
                     /translation="MIINLNDYFDPVCIEKPDYEFISGASSFSHNITIHTDNNPITGI
                     EKYRIAFIGVPEERSSVNNGCQKGPEAIRRSLYMLARIPGKLKIIDLGNMKRGTNFSD
                     TVSGLSEIISSLIELKILPVIIGGNSSLITAIDSSFSKLRHPYTLTSIDSRIDFQPEK
                     KETDSFSYLNNILYNSKSTLINLINIGYQTYLNDQQVINRLIRRKADLMRLGDVRDAI
                     HLTEPFLRDSDAIVIDISSVRQSDAPGTILPSPNGFYGEEICLIARYAGISDKIKVFG
                     VFEVNPDLDKGSQTSGLAAQILWFFLEGFCQKQIETPSLAKHNTGQFTRYHVHISGTE
                     EELIFIKSNLTERWWIEINTDKGETKYVACSYEDYVQANHNEIPQRWIKAIERLSK"
     gene            5348..6292
                     /locus_tag="PLG42_06125"
     CDS             5348..6292
                     /locus_tag="PLG42_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOU30818.1"
                     /translation="MKKYGILFIFALILAGRSFSQQDPVYSQYMYNFHFFNPGSAGIS
                     GMVCMTATSRQQWLGFKGAPSTTGFSVNAPVRLFGSESGIGMVIMNDKTGFDSDIKLS
                     GSYSYHFELGNGKLGVGISLGMMNKTLNPSWQIPESDAHTPVSGDPLIPENKESHIAF
                     DAGVGAYYSTEKYYAGISITHINEPKIKFSKGEPYLSRHYYATAGYSLNLGNPSYELI
                     PSVFVFSDGHLVQFTINSMLRYNKKVWGGVSYRAGDALTGIVGIELYNGIRIGYSYDF
                     ILSDIRKSSSGSHEFMVNYCFDISLGKSPMKYKSVRFL"
     gene            6339..>6948
                     /locus_tag="PLG42_06130"
     CDS             6339..>6948
                     /locus_tag="PLG42_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU30819.1"
                     /translation="MKKVIFFALILIILLNSCGPSMTGELTGVPGRKKFFEPEPYQMA
                     FIPAGSFVMGPSDEDAFHVMNTMSKTITVEAFWMDQTEITNNKYRQFVYYVRDSIFRT
                     KLAEAGIEGREYYMTDKEGNVLEPKTLNWDEKIDLRDEQTLAILEEMYYPPQERFNGR
                     KEIDTRKLNYSYYFVDYKQAAQVPYVQDYKAGTGKYVGEAYNL"
BASE COUNT         2276 a         1358 c         1345 g         1969 t
ORIGIN      
        1 ttccacctta taaaagggtt aaaaaccatt ttggatggca agagtataaa gaaacggttt
       61 tattgtactg ccaacctgtc ttttttgttc tgtaacagca tcgtacttga aatatctgaa
      121 gtccggacct cccacataag ccttaatgtg cccggtatgt gggtccataa tcataaatga
      181 cgaacgcatg aaatgtttat aataccttat tgaatcaaga ggggtcataa cagtatcccg
      241 ttctcctttc caggagaaga ccctcatctt aacaggagtg ttgaatgcca gcattataga
      301 atcttccgga actttccttc ttaccatttc cctgtacctg tcggttctcc tgatagccga
      361 cataagtatt tcttctacct gcttttttgt taaatcattg gaaaaaggtg gatttttaaa
      421 agtttttgcc agcctgtcaa acgcaggctg tatctcctta agatgttccg tcagtgcctc
      481 ctccgcatat ttctgcatcc tggaatttat agtagtatat atcttcagtc cgtctttata
      541 aaggttgtag tttgaaccat ctggtttttt attcttcctg caccatccat aaagcggatt
      601 attctcccat tgccatacag catcattata ttgttcctca taagaaaaaa atcgcctgtc
      661 aggttcaaac ttgatcattg tagtccgtat atattcccgc agatatgtag caagaccagt
      721 attgtgactc tcttcagtgt aattgaggcc aagaggaagt ttcttgaggg aatcggcctc
      781 ggcataggtg atatattgat actttgccat ctggtcaaga acaacatttc ttcttgccag
      841 tgtcagctca ggtcttttca acggattgta tctggatgag tttttcagca tacctatcaa
      901 cattgctgat tgttcaatgg ttagagaatc cggagtggtg ttaaaataaa ttcttgcggc
      961 tgaccttatc ccaacggcat tgttaacgaa atcaaaaacg ttgagataca tggcaataat
     1021 ttcttctttg gtataattct tctccagctt aacagctgta taccactcct tgaacttgga
     1081 tattacaaga tttacctttc gctttaccct tgagtacctt atggagtcaa ctccacccct
     1141 ggttttaaaa agattttttg caagctgctg agaaattgtg ctacctccgc ctgcttcctg
     1201 ctgtaaaaga atcgacctga taaaaaccct tcccagccct cttatatcaa tacctgaatg
     1261 cctgtaaaac ctgatatcct ccgttgcaag aagtgcattt attacatttt tcgatatatc
     1321 cttataatcg gtccaggtac ggttctctat gtaaaacttc ccgaggagaa ctccgtcttc
     1381 tgagtaaact tccgctgcca gattattttc cgggttctca agctcctcaa atgttggcat
     1441 cggaccgagc ttctctgccg aaataagtaa aaacattact ataataaaaa tgtatggcag
     1501 agcaataatt ccccagaacc atattagata tttcctatat tttttatttt cctccttcat
     1561 cacctgaccc tgaaaatcgt tgccaaaaat agtaattata atataaaaat taataataat
     1621 atagccgagt gatttgaaga ttgattttga aatgatttac tttgtcagat tttaaataga
     1681 gtcaaaccgt gtcatccatg attgaaaacc ttgtaatcgt tgaatctccg gcaaaggcaa
     1741 aaacaattga gaagttcctt ggaaaggatt tcagggttgt ttcaagtttc ggacatatca
     1801 gagatctgac aaaaaagaac ctcggaattg atataaaggg agggtttata cctgattacg
     1861 aaataccaaa agataaaatt aaggtagtta acgagctccg caaagcagct tcagaagcca
     1921 aaactatctg gatcgcctcc gatgaagacc gtgaaggtga agctatagcc tggcatcttg
     1981 ttgaagtgct aaatctcgac ccacagaaaa ccagaaggat agtttttcat gaaatcacca
     2041 aaagtgccat tgaagaggcg ataaagtctc cccgaactgt tgatatgaac cttgtaaatg
     2101 cccagcaggc gagaagaatt cttgaccgtc ttgtgggttt tgaaatctca ccggttctct
     2161 ggaggaaagt tcagcctgct ctctctgcag gccgtgttca atcggtggct gtacgtctga
     2221 tagttgaaag agaaagggaa ataattaact tcaagcctga atcttcattc agaataacag
     2281 gcattttcaa taaagataaa tcttctgaca atttattagt tgaagaacct cctctgaagg
     2341 ctgaagccat cacaaaattt catgatgaag atgaagctgt aaaatttctt gagctttgca
     2401 gggaagctgt atttactgtt aaaaatgtaa tcgtaaaacc cggtacccgt tctcctgcgc
     2461 ctccgtttat tacttccacc ctccagcagg aggcctaccg taagctcggc ttctccgttt
     2521 cccagaccat ggcaatagca cagaaacttt atgaggaagg aaagatcaca tacatgcgaa
     2581 ccgattcaac aaacctttca cgaatggcac ttgcaaaaat ccgtgaggta attgtaaatg
     2641 aatacggtga aaaatattca aaagtaagac agtacaaaac aacatcgaaa ggagcccagg
     2701 aagcacacga agcaataagg ccaacctacc cggaaaaatc cacaatatca ggaactcagg
     2761 ctgaaaaacg attgtatgaa ctcatctgga aacgaactat cgcctcccag atggctgatg
     2821 cccagacaga aaaaacaaac attgccattg aaatgaacaa ttcaccggtt cttttcaatg
     2881 caacgggaga agtaatactt ttcgacggat tcctgaaagt ttattccgaa tcacaggaca
     2941 ccgagggtgg tgaagacgaa aaggcaatac tgccatcagt taaaccaggt atgttacttt
     3001 tctatgacag aattaccgca atcgaaaaat atactactcc gccgccccgg tataccgaag
     3061 caagccttgt aaaaaaactc gaagagctgg gaataggacg cccttcaaca tatgctccta
     3121 tcatctcaac tattcagcag agaggatatg ttgcacgtga aaacaggccg ggagaaaaaa
     3181 gaaaaataac agttctggag ctctctgcag gaaatatcac caaatcaaca aaaaccgaaa
     3241 tcttcggaaa agaaaaatca aaacttttcc ctaaagacat agggatgatt gtcaatgatt
     3301 ttctaatgga aaatttttcc gaaatcgtag attataattt cactgctgaa gttgaagaac
     3361 agttcgacaa aattgcagcg ggaaaacttg catggaataa aatgctcgag aatttttacg
     3421 gccctttcca taaaaccgtg gagaatactc ttgagaaaaa gaacaaaacc accggaacca
     3481 gaatactcgg aactcacccc gaaacaggtg aaactatttc cgtgaaaatg ggaaaatacg
     3541 ggcctgttgt tcaggcagta catcctgaaa aaggcgataa accccgattt gcaagccttc
     3601 agaaaaacca gcttatggaa accataacac tcgaagaagc aatgaagctt ttctccctgc
     3661 caagatccct gggaaactgg gaaggcgatg aggtggttgt aggaacaggc aggtttggac
     3721 cttatattag gtacagaaat aaattttatt ctctgaagaa tattgacgac ccctacctta
     3781 ttacacttga acgggcaatt gaaataataa aagaaaaaga tgaagctgat aaacaaaaag
     3841 taataaagga gtttggagaa ataaaaataa tcaacggtag atatggagca tatattaatt
     3901 acaggggcga aaattacaga attccaaaag gaacaaatgc tgaggacctt acacaggaag
     3961 aatgcatgaa cattattgag aaaaaacaaa agtcgggaaa atgataatca atcttaatga
     4021 ctattttgat cctgtatgca ttgaaaaacc tgattatgaa tttatatcag gggcttccag
     4081 tttttcccac aatattacaa ttcataccga taataatcct atcaccggta ttgaaaaata
     4141 caggatcgct tttatcggtg ttcctgagga gcgtagttct gtaaataacg gatgccagaa
     4201 gggtcctgaa gcaataaggc gatctcttta catgctggcc aggattccag gcaaactgaa
     4261 aattatagac ctcggaaaca tgaaaagagg cacaaatttt tccgacactg tttccggtct
     4321 ttcagaaata attagttccc ttattgaatt aaaaattctc ccggttatta tcggaggcaa
     4381 cagttcactg attacggcaa tcgacagctc tttttcaaag ctcaggcatc cttatactct
     4441 cacctcaatt gattcccgaa tcgattttca acctgaaaaa aaagaaacag actcattcag
     4501 ctatctgaac aatattttat ataatagtaa aagtacatta ataaatctga taaacattgg
     4561 ctatcagact tatctaaacg atcagcaggt aataaacaga ttaataagaa gaaaagctga
     4621 cctgatgaga cttggagatg taagagatgc cattcatctt acagaaccat ttcttagaga
     4681 ttcagatgct atagtaattg atatttcatc agtgcgtcaa tcagacgctc ctggtacaat
     4741 cttaccttca cccaatggat tctatggtga agaaatatgt cttattgcaa ggtatgcagg
     4801 tatttccgac aaaattaagg tttttggggt ttttgaagtc aatcccgatc ttgataaagg
     4861 ttctcagacc tctggccttg ccgcccagat cctgtggttc tttcttgagg gcttttgcca
     4921 gaaacaaatt gaaaccccat cccttgcaaa gcacaataca ggacaattca caagatacca
     4981 tgtacacatc agtggaactg aagaagaact aattttcatt aaaagcaacc ttacagagcg
     5041 ctggtggata gaaataaata cggataaggg agagacaaaa tatgtcgcat gctcttacga
     5101 agattatgta caggcaaatc ataacgagat acctcaaaga tggatcaaag caattgaaag
     5161 attatctaaa taaaaaaatg ttaattttat ccgttcatcc ataatttttt gacgttcatc
     5221 actaattatt attactctta tcaaaataat cgcattacaa caaaaaataa atatttttat
     5281 atttgtcagc tattcatatt tttatttaat tttactgatt tagattttga ggcatcaggt
     5341 taaaagaatg aaaaagtacg ggatattgtt tatttttgct cttatactgg cgggcaggtc
     5401 attttcccag caggatcctg tatacagtca atatatgtat aattttcatt tttttaatcc
     5461 tggttcggct ggtatttcgg ggatggtttg catgactgcc acctcaaggc agcaatggct
     5521 tggtttcaaa ggtgccccat caacaacagg atttagcgta aatgcaccgg tcagactttt
     5581 cggttcagaa agtggcatcg gaatggtaat aatgaacgac aaaactggtt ttgattcaga
     5641 tattaaactt tcaggctcat attcatatca ctttgaatta ggaaatggta agcttggagt
     5701 ggggatatca ctgggaatga tgaacaagac tctgaatcct tcatggcaaa tacctgaaag
     5761 tgatgcacat acgccagttt cgggagatcc tcttattcct gaaaataagg agagccatat
     5821 tgctttcgat gcaggagtgg gagcatatta ttcgacagaa aaatattatg ccggaatatc
     5881 cataactcac ataaatgaac ctaaaataaa attttcaaag ggcgaaccat accttagcag
     5941 gcattactat gctacggctg gatacagtct gaatctcggc aatccgtcat atgaacttat
     6001 cccatcggta tttgtattta gtgatggaca tctggtacaa tttacaataa actccatgct
     6061 cagatacaat aaaaaagtat ggggcggcgt ttcttacaga gccggtgatg cactgacagg
     6121 tattgtcgga attgaacttt acaacggaat tcggatagga tattcatatg attttatcct
     6181 gtctgacatc cgcaaaagtt caagtggttc tcatgaattt atggttaatt actgctttga
     6241 tataagcctg ggcaaaagtc ccatgaaata taaaagtgta agatttttat gattattaat
     6301 aaaaaattac ttacttgcat ctaaaatcat attgaattat gaaaaaggtg attttctttg
     6361 ctctgatcct gattattctg ctaaatagct gtggtccgtc aatgaccgga gagcttacag
     6421 gtgttcccgg aaggaaaaaa ttctttgagc cggaacctta ccagatggca ttcatccctg
     6481 cgggaagttt tgttatgggc ccgagtgatg aggatgcttt tcatgtaatg aacaccatgt
     6541 caaaaactat tacagtggaa gctttctgga tggaccagac agaaattacc aataataagt
     6601 accgccagtt tgtttattac gtccgtgatt ctattttcag aaccaaactg gccgaggcag
     6661 gtatagaggg acgtgagtat tacatgacag acaaggaagg gaacgtactt gagcccaaaa
     6721 ctctcaactg ggatgaaaaa atagacttac gcgacgaaca gactcttgca atacttgagg
     6781 agatgtatta tccgccacag gaaagattta atggcagaaa agaaatagat acaagaaaac
     6841 tcaattattc atactacttt gttgattata aacaggctgc tcaggtgcca tatgttcagg
     6901 attataaagc cggaaccgga aaatatgtag gtgaagccta caatcttg
//
LOCUS       DAOSNB010000122         6936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_10977_length_6936_cov_4.709781, whole genome shotgun sequence.
ACCESSION   DAOSNB010000122 DAOSNB010000000
VERSION     DAOSNB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10977_length_6936_cov_4.709781"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1701)
                     /locus_tag="PLG42_06135"
     CDS             complement(178..1701)
                     /locus_tag="PLG42_06135"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOU30820.1"
                     /translation="MAIIQQKGFDNEKYLSEQTSAILERVNRFDQKLYLEFGGKILYD
                     YHAARVLPGFDPNVKMRLLQSLKDKTDVILCIHAGAIERNKIRADFGITYDVDTLKTI
                     DDFREWGIDIKAVVITRYQNQAPARVFKNRLELRGIKVYVHNPINGYPADVDFVVSDK
                     GFGANEYIETTKPIVVVTGPGPGSGKLATCLNNLYHEYKRGIRAGYAKFETFPIWDLP
                     LDHMVNLAYEAATVDLGDRVMIDEHHLNAYNIKTVNYNRDIEAFHLLKRIIEKITGGK
                     SMYLSPTDMGVNRVSAGIIDDKIISEAAYQEIIRRYFRCLMEYMMGLAEREMLDRINA
                     IMIRIGARPEDRRVVLPAREAALDAEKRGKGNDGVFSGGAIELPDGTIITGKNSPLMN
                     ASSSLVLNATKHLAGLPDKMYLLPESIVNSVTHLKKDILHGRMVSLDLDETLIALGIS
                     AISNPAAKMALENLKLLRDCEVHLTHIPTPGDEAGWRKLKVNLTCDPEYSGKSLFIS"
     gene            complement(1829..2671)
                     /locus_tag="PLG42_06140"
     CDS             complement(1829..2671)
                     /locus_tag="PLG42_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOU30821.1"
                     /translation="MIDSLITMSVAGLIAGFLFSIPSAGPISILIISNALKGKLGYCI
                     RANMGAAVADFIYVLIGTFGLTKLYSLYKPAIPYFLLAGMLFLLVTGYKITRIKMDPE
                     HLPEINTVKKIKHRGAFYTGFMVNFLNPALFVGWLTISFLAISFVASLGFNTGGLNAV
                     VNQNVTSIDNITGLKIQEQKILSNKYFDKIYNHNKREQNFRKDDPSAYPGYFHFIISS
                     VYAFFLSLGSVIWFHFLSLIFTKFRKYINLKIVNRIIFGMGVVLCIFGLYFGYIAIKT
                     FSRI"
     gene            complement(2664..3413)
                     /locus_tag="PLG42_06145"
     CDS             complement(2664..3413)
                     /locus_tag="PLG42_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOU30822.1"
                     /translation="MKAVILAAGIASRMRPLTNTTPKCLLKAGNKFILELTIENILAN
                     GISDIIIVTGFLEDQIRDFVKSCFPAINVTFIYNPYYNSTNNIYSLWLTEEAVMGDDM
                     LLTDSDIVFEKEIISKLISSGYKDCLALSRHELHEEEIKVKLHEEGYILEISKEVDPS
                     VAAGESVGIEIFGKKTLPTLYEILNRKINIEKNVTQFYESAFQELINRGHRIYAVDCS
                     EYFCMEIDTISDLETVNSLIAEKHLKALEND"
     gene            complement(3413..4288)
                     /locus_tag="PLG42_06150"
     CDS             complement(3413..4288)
                     /locus_tag="PLG42_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOU30823.1"
                     /translation="MRWVNEYKKSLKLLEVEEILDVYFYRPLAFLVVKLVYPTRITPN
                     QLTFAAISAGVTAGFFYASGTRKGSIIGALFFLLFNILDCSDGQLARLKKNGTPEGRI
                     IDGIADYISEFAVFAGIAIGFHKSSDQPHFLIILLVLSGMSIMVQNIMVDYYRTRFLD
                     YVQGRTKTLKEGIDEYEKEYALLKNQKGSLMKKLIISLYLKYSALQSWFTVKRRSKEK
                     IFSSPAEYYRKNRIIIRFWVLIGPTAQITAIIICSFFCRFDIFFWLILAGFNSLALVL
                     RIIQKRIDRSLIASH"
     gene            complement(4285..5355)
                     /locus_tag="PLG42_06155"
     CDS             complement(4285..5355)
                     /locus_tag="PLG42_06155"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU30824.1"
                     /translation="MVNIGIKKQLIFLDRNENNYGPAPACKKVLRRRSNGFLNIYSRD
                     FTRGVKSVLSERLSQDTGIPESRILLGYGSEDILKQVVQCYLKDGGKILIPSYSWWYY
                     KKIAAEASGVNIEYPIIEIEKENRFEYDIDILLKTYREKKPAIILLSSPNNPTGNRLE
                     IKQLKLILDNTPDCTVILDEAYSKFDPGFNVNAAEFVEKYPQLIIIRTFSKYYALAGL
                     RIGYALIGARHEHLQLLSTRYLGFNRLSEQIAIAALNSSDYYRKIQRKMVQDREMLYN
                     EFNALPGFKAYVSYANFILVRIPAEIKESLQKYLTDRDIIIKFMNEDNLNSHVRITIG
                     TTSQNRKLINAVRNFMNERSRS"
     gene            complement(5520..5990)
                     /locus_tag="PLG42_06160"
     CDS             complement(5520..5990)
                     /locus_tag="PLG42_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30825.1"
                     /translation="MKKILLVLITVICVITAVNAQDYKTGIGLRGGFFNGLTVKHFVK
                     SNAAIEGLLFSRWKGFEITGLYEFHAPAFQVDRFKWYFGPGAHIGFYNGDNTTWGEEG
                     KTYTNLGIDGILGLEYSISQIPINIGLDWKPEFNIIGYSGFWYDAVALSIRFIF"
     gene            6175..>6936
                     /locus_tag="PLG42_06165"
     CDS             6175..>6936
                     /locus_tag="PLG42_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU30826.1"
                     /translation="MDIVIQNITKSYGVQKAVDNISFKVNTGEIVGFLGPNGAGKTTT
                     MKIITSYLAPDEGDVFIGGRSVRDNPVETKKYIGYLPENNPLYYDMPVIDYLEFCARI
                     QGIEKSRVEARVAEMIRITGLNREKHKKIGELSKGYRQRVGLAQAMIHDPQILILDEP
                     TSGLDPNQIAEIRKLIRELGREKTVILSTHILPEVEATCDRIFIINKGKLVADGTPDT
                     LRKKAQGADILRVKIEDGNPDTIFKALKTLDSVTMV"
BASE COUNT         2144 a         1439 c         1184 g         2169 t
ORIGIN      
        1 ttgctatcgg gatgagctat tcccgcgttt tttgggttat gctctacctc ccgataattt
       61 attcccgatt tatatcgggg aggaatgaac ccttttcgta aaatgccgga caaaattaat
      121 aaaaaatttt attcttcaaa tacgcagttc agtatttact gaacaccttt tctataatta
      181 actgataaac aaactcttgc ctgaatattc cggatcacat gtaagattga ccttcagttt
      241 tctccatcct gcctcatcac ccggtgtggg tatatgtgtc aggtggacct cgcagtcgcg
      301 taacagtttc aggttttcaa gagccatttt tgctgccgga tttgagatag cacttattcc
      361 aagggctata agagtctcat caaggtcgag gcttaccatt cttccatgca ggatatcttt
      421 tttcagatgg gtaactgaat tcactatgct ctcgggcaga aggtacatct tatctggcag
      481 gcctgcaaga tgcttggtcg cattcagaac aaggctggaa gatgcattca tcagagggga
      541 gtttttcccg gttattattg taccatcagg caactcgatt gcgccgccgg aaaagactcc
      601 gtcattacct ttgcctcgct tctcagcatc gagtgcagct tccctcgcag gcaaaacaac
      661 cctgcggtct tcgggacggg caccgattct tatcatgatg gcattaattc tgtcgagcat
      721 ctctctctct gcaagcccca tcatatattc cattaagcat ctgaagtacc ttcggataat
      781 ttcctgataa gccgcttcgc taattatctt atcatcaata atacctgcac ttacgcggtt
      841 tactcccata tcggtagggg aaagatacat tgacttacca ccggtaatct tctctataat
      901 cctcttaagg aggtgaaaag cttcaatatc cctgttataa ttgactgttt taatattata
      961 cgcattcaaa tgatgctcat caatcatcac cctgtctcca aggtcaacag tcgcagcttc
     1021 ataggcaaga ttaaccatat gatccagtgg caggtcccat atcgggaaag tttcaaattt
     1081 tgcatatccc gccctgatac ccctcttata ctcatgatac aggttattca ggcaagttgc
     1141 cagttttccg cttcccggac cgggaccagt tacaactact ataggtttag ttgtttcaat
     1201 atactcattg gcaccaaatc ctttatcact aactacaaag tcaacgtcgg caggataacc
     1261 attaatagga ttatgaacat aaacctttat acccctgagt tcaagcctgt ttttaaacac
     1321 ccttgcaggc gcctggttct gataacgtgt gattacaact gctttaatat caatacccca
     1381 ttctctaaag tcatcaatag ttttcaatgt atccacatca taagtgatgc cgaagtctgc
     1441 acgaatcttg ttcctctcta ttgcacctgc atgaatacaa agaataacat ctgttttatc
     1501 tttcagactc tgtaagagtc tcatctttac attgggatca aaacccggaa gtactctggc
     1561 agcatgataa tcataaagaa tcttcccacc aaattcaaga tacagctttt gatcaaagcg
     1621 gtttaccctt tcgagaatgg ctgaagtttg ttcgctcaga tacttctcat tgtcaaaccc
     1681 tttctgttga ataatagcca ttttcgatta aaaatttaaa aaagttaatg aaaataaaat
     1741 tttctttcaa atgaaaactt aagaacttgc ttggtatttg ttgttttcaa attttgcccg
     1801 tttcacctat cctgccttta ataaaaacct atatccttga aaaagttttt atagcaatat
     1861 atccgaaata tagaccaaat atgcatagta ctacgcccat tccgaaaatt attctattca
     1921 caatcttcag attaatatat ttcctgaatt ttgtaaagat taatgaaaga aaatgaaacc
     1981 agataacgct gccaagtgaa agaaaaaagg cataaactga actgataatg aaatgaaaat
     2041 atcccggata tgcacttggg tcgtctttcc taaaattctg ttcccttttg ttatgattat
     2101 aaattttatc aaaatactta ttggataaaa tcttttgctc ctggattttt aagccggtaa
     2161 tattgtcaat gctggtaaca ttctgattta ctaccgcgtt caatccaccg gtattaaatc
     2221 ccaaagatgc aacaaatgat attgccagaa aagaaatagt aagccatccg acgaacagag
     2281 caggatttaa aaaatttacc ataaaaccag tataaaaagc acccctgtgt tttatctttt
     2341 ttacagtatt aatttccggc agatgttcgg ggtccatttt gatccgcgtt atcttatatc
     2401 ctgtaaccag caggaacaac attccagcca gaagaaaata tggaatagca ggtttataaa
     2461 gagaatagag ttttgtcagt ccgaaagtgc caataagtac ataaataaaa tctgcaacag
     2521 ctgctcccat gtttgccctt atacagtaac caagcttacc cttaagtgca tttgaaatta
     2581 tcagtatgct aatagggcct gccgaaggta tcgagaaaag aaagccagca atcaaaccgg
     2641 caaccgacat ggttatcaga ctatcaatca ttttccaatg cttttaaatg cttttcagct
     2701 attaatgagt taactgtttc aagatctgaa attgtatcaa tttccataca aaaatactct
     2761 gaacaatcta cagcataaat tctgtgacct ctattgatca gttcctgaaa agcagattca
     2821 tagaattgag ttacattctt ttcaatattt atttttctat tgagtatttc atataatgtg
     2881 ggtaatgtct ttttcccgaa tatctcaatt cctacagatt cacctgcagc cacggaaggg
     2941 tcaacttctt tgctgatttc cagaatatat ccttcctcat gtagttttac ctttatttct
     3001 tcttcatgca attcgtgccg gcttagtgca agacaatctt tgtatcctga agagatcaat
     3061 ttgcttatta tctccttctc aaaaactatg tcactatcgg tcaggagcat atcatccccc
     3121 atcacagcct cttcagtaag ccaaagggaa taaatgttat tggtagaatt gtaataaggg
     3181 ttataaataa aagttacatt aatggcagga aaacaacttt ttacaaaatc acggatctga
     3241 tcttcgagaa aaccagttac tattattata tcactaatac cattagccag aatgttttct
     3301 atagtaagtt caagaatgaa tttattgcct gcttttagaa ggcatttggg tgttgtgtta
     3361 gttagaggcc tcatccgtga ggcgattcct gctgcaagaa taactgcttt catcaatgag
     3421 atgcaataag agatctgtca attcttttct gtattatccg caaaactaat gctaaggaat
     3481 taaatccggc aagtatcagc cagaaaaaaa tgtcaaaccg gcagaaaaag gaacatataa
     3541 tgatagctgt aatctgtgcc gtcgggccta tcagcaccca gaagcggata atgattctgt
     3601 ttttcctgta atattcagcc ggagatgaaa atattttttc tttacttctt cttttcactg
     3661 taaaccagct ctgcaatgca gaatatttca gataaaggct tattatcagt tttttcatca
     3721 aacttccctt ctgattcttc agtaaagcat attctttttc atactcatca attccctctt
     3781 tcagtgtttt tgttcgtccc tgaacataat caagaaaacg agtcctgtaa taatcaacca
     3841 tgatattttg taccattata ctcattcctg aaaggacaag cagtatgatt aaaaaatgag
     3901 gctgatccga gcttttatga aatcctattg ctattccagc aaaaactgca aattcagaga
     3961 tataatctgc tataccgtcg attattcttc cttccggcgt tccatttttt ttcaagcggg
     4021 cgagttgtcc gtcactgcaa tcgagaatat taaaaagcag aaaaaagagg gcaccaataa
     4081 tcgagccttt tcttgttccc gaagcataaa aaaagcctgc ggtaactcct gctgaaattg
     4141 cagcaaatgt caactggttg ggggtaatcc ttgttggata aaccaatttt acaacaagga
     4201 acgcaagcgg ccgataaaaa tacacatcaa gaatttcctc aacttcaagt aacttaaggg
     4261 atttcttgta ctcattcacc catctcatga tctgcttctt tcattcataa aatttctgac
     4321 agcattaata agtttacggt tctgcgaagt tgtcccgatc gtaatacgaa catggctgtt
     4381 cagattgtct tcgttcatga atttgattat tatatccctg tctgtcagat atttttgaag
     4441 agattcttta atttccgcag gaattctgac gagtataaag ttggcatatg aaacatacgc
     4501 cttgaaaccc ggcaaagcgt taaattcatt gtatagcatt tctctatctt gtaccatttt
     4561 cctctggatt ttcctgtaat aatccgaaga attgagagca gctattgcta tctgttccga
     4621 caggcggtta aatccaaggt aacgggtact cagaagctga agatgttcat gcctggcacc
     4681 tattaaagca tatcctatcc tcaatcctgc aagtgcataa tattttgaga aagttcttat
     4741 aataatcagc tgcggatatt tctcgacaaa ttcagcagca ttaacattaa accctggatc
     4801 aaacttcgaa taagcttcat caagaatgac agtgcaatcg ggggtattat caagtattag
     4861 tttaagctgt ttaatttcaa gacggttacc ggtcggattg tttggagaac ttaaaagaat
     4921 tatagcaggc tttttctctc tgtaagtttt caggagtata tcaatatcat attcaaatct
     4981 attctctttt tctatttcta ttattggata ttcaatattt actccgcttg cttctgctgc
     5041 aatttttttg taataccacc acgagtaaga cggtataagt atcttaccgc catctttcag
     5101 atagcattga actacctgct taagaatatc ctcactacca taaccaagca atatcctgct
     5161 ttctggtatt ccggtatctt gtgaaagcct ctcggaaagt acactcttta cacctcttgt
     5221 aaaatccctc gaatatatgt ttaagaagcc attacttctt cttctcaaaa cctttttgca
     5281 tgcaggagca ggtccataat tattctcatt cctgtcgaga aaaattaatt gcttttttat
     5341 tccaatatta accataagat gtttttctcc aagatttttc ccctctgtaa aatttctata
     5401 aaaataattt taaacaaaca atattaatca tttctttttc agatttgtaa aattttaaaa
     5461 aacaggtggt tgaaaaaatt tcaaccacct gaattaatat agtctacaat tatcttcagt
     5521 taaaatataa accttattga taaagccacg gcatcatacc agaatccgct ataaccaata
     5581 atattaaact ccggtttcca gtcgagtcca atatttatag gaatttgact tatgctgtat
     5641 tcaagtccaa gtattccgtc gatgcctaaa tttgtataag ttttaccttc ttcaccccaa
     5701 gttgtgttat caccgttata aaatccgata tgtgccccag gaccgaagta ccatttaaac
     5761 ctgtctacct gaaatgcagg agcatgaaat tcgtaaagtc ctgtgatttc aaaacccttc
     5821 cagcgtgaga aaagcagacc ttcaatagct gcattactct ttacaaaatg cttgacggtg
     5881 agcccgttaa aaaaaccgcc tcttaaacca atgccggttt tgtaatcctg ggcattaact
     5941 gcggttatta cgcagataac tgtaataaga accaataaaa tctttttcat aagtacttgt
     6001 tttaaaattg cccaaagata ataaatttat catgttctat gctgtatcct gagaatcaat
     6061 aactatacca ttctttccca ttttaacaaa tctttaacta tgaagcattt ttatttttta
     6121 actgtttgaa ataatttcgg ggcgtctttc catgtttcta aaaactaatt acatatggac
     6181 attgtaattc agaacatcac aaaatcttat ggtgtccaga aagcagtcga taatatatca
     6241 tttaaggtaa atacaggaga aatagtcggt tttctgggac ctaacggtgc aggtaaaacg
     6301 acaacgatga aaatcatcac cagttatctc gcccccgatg aaggagatgt gtttattgga
     6361 ggaagatcgg tgcgggataa tccggttgaa accaagaagt atataggcta tctgcccgaa
     6421 aacaatcctc tttattatga tatgcctgtt attgattacc tcgaattctg tgcaaggatt
     6481 cagggtatag agaagagtcg tgtggaagcc cgggtggcgg agatgataag gattaccgga
     6541 ctaaaccgtg aaaagcataa aaagataggc gagctttcga agggatacag gcagagggta
     6601 ggtctggcac aggcaatgat acacgaccct cagatactga tactcgatga gcctacctca
     6661 gggcttgatc ctaatcagat tgcagaaata agaaagctca tccgtgaact gggccgcgaa
     6721 aagacagtaa tcctcagtac ccatatcctt cccgaagtgg aagccacctg cgacagaatt
     6781 tttatcatca acaaaggcaa actcgttgcc gacggaaccc ccgatacact cagaaaaaaa
     6841 gcacagggag ccgacatcct gagggtgaaa attgaagacg gcaaccctga tacaatattc
     6901 aaagcactta aaacccttga ttcagtgaca atggtc
//
LOCUS       DAOSNB010000123         6912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11014_length_6912_cov_5.403967, whole genome shotgun sequence.
ACCESSION   DAOSNB010000123 DAOSNB010000000
VERSION     DAOSNB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11014_length_6912_cov_5.403967"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLG42_06170"
     CDS             complement(<1..340)
                     /locus_tag="PLG42_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30827.1"
                     /translation="MVRIINLTGGLISYLTSLLVHRAVVYGMPAALNVEISGKCNLRC
                     PECLTGSGQSKRPSGFMEVELYKKILSELKSYLLNVNLYFQGEPMLHPDFFKFVSLSR
                     GIRLTLSTNGH"
     gene            509..1822
                     /locus_tag="PLG42_06175"
     CDS             509..1822
                     /locus_tag="PLG42_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU30828.1"
                     /translation="MKKLSIYPGIVLTILLVLSTAFPVLAGKPKATKNPDQQSILVLR
                     GRVVDKETLQPLIFASVAVKETNVATITNIDGEFIIKIPETEVSKNLEITHIGYKNKV
                     IPLIELKDDGFRNIITMESAPVPLKEIIIKPIMPDEIISKLLSSISKNYPNIPNMMTA
                     FYRETIRKNRQYISIGEALVEIFKAPYQNDLRFDATRIYKGRKNTDMAKSDTVLFKLQ
                     GGPVTALELDLIKNPEAILTPEAIKYYDYRLTGMVEIDERPNYIVEFSQKKDITMPLF
                     MGKLYIDTKTFALSAAEFGLNLENKEEATALFIKKKPLGMEVTPEVASYMIKYREQDG
                     KWYFAYSRAEVRFKVNWKRKLFNPHYTTMSEIAVTDRTEEEVIKFAGKEKLRYTDIFL
                     DEVSAFADENFWGEYNIIEPDQSIEQAIRRLARKVKFSDRPPEEK"
     gene            1834..2367
                     /locus_tag="PLG42_06180"
     CDS             1834..2367
                     /locus_tag="PLG42_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOU30829.1"
                     /translation="MPEEREIIRRIREGDIKEFETLFRSSYISLVKYAGTLVKDRDTA
                     EEIVQDLFVKIWNERKNLNIKSSLTGYLFRSVHNRCLHHLQHLKVVEKHAERIYETNT
                     GIADEADGEINLKELEDKIARVLEKLPERCCKIFCMNRFEGLKYKEIAEKLSISVKTV
                     EACMGKALKELRKIIED"
     gene            2373..3359
                     /locus_tag="PLG42_06185"
     CDS             2373..3359
                     /locus_tag="PLG42_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOU30830.1"
                     /translation="MKPSEKFTDQYWERLAARFSGEETTGDEFSGNVSSETEKMLERY
                     WKSAGSEINSEEINIESAWNIVRSKISSEKVHPHQAGVRKSISLYLRIAAAVLLLAAL
                     GTVTYVLFDKAVSGSRIIIATGEEEKNREIVLPDGSKVWLNHSSEISYSKNWNRTGRK
                     VTLSGEGYFDIVPDEKNPFSVDAGKAEIKVVGTSFNVITRNLEGEVEVFVESGKVLLA
                     DEKGGSILLEPGYIGTTAPGRLNKTLNNNRNYLSWKTSLLVYEGTSLTDVFRDLKRAY
                     NIDISVADSSILDKTITAVFNNDPVETIIRVICTTFNLSYIKDGKHFHLLKN"
     gene            3372..4931
                     /locus_tag="PLG42_06190"
     CDS             3372..4931
                     /locus_tag="PLG42_06190"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU30831.1"
                     /translation="MKNCNVWLVRKKTGIALLVLLCCSIWLNGQENILDSLFTFKSGT
                     VKVSNAFNIISRQTGYSFTYDSKLINPDKKVSLTFQRTQLREILNQIISNDSVDFTVI
                     NKYIIIYKITPYQPYAIEIPEWEYKDLTGTISDFETGEPIPYATIGIRSVGRGTVANN
                     NGEFLFKINRDWIKDTLVISHIGYQQRKIPVWQAIGNEFNIKLKRDYIPIPEIIIRNQ
                     PPQEILRKAYTYVSRNYGNTPAMLTGFYREAVIKKNDLQIYSEAIIGIYKSPYTSSLL
                     GDQIKIFKSRKMENIGLKDTITVRLKAGLNSCLELDGAKNTFDFLYPENFSQYDYRMT
                     DIVKVDEGTAFVVEFVQKPEVEIPLFTGSIYINTLNYAIEYAEFEVNPAYIKKNKEDF
                     ITYQAKGYTILPISIKYRVSYKNINGRYFLNHVRGDLNFTARQKNHLFTTNFLVFFEM
                     AVTDIRTENVTRFERDETAPLQSVFSKTITGYDYDFWGDFDFLKPEDNLLNELNNMKA
                     RLQIFSKEEGK"
     gene            complement(5001..6011)
                     /locus_tag="PLG42_06195"
     CDS             complement(5001..6011)
                     /locus_tag="PLG42_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU30832.1"
                     /translation="MSKIKGLILLAVSVLAVSSCVPGTKYKSMQDTAKMYMNERDELK
                     TENIMLAMERKELEARVQKLEEETKSMSGELTALRTERDRLKEEYNILQGKVNDLEKA
                     REDLIKGNVTETRRLLSELQAAQENLQKKEDLLRQLEANLDMKKASLDELSYELEKRN
                     ARMAELEKILETQKRIVQELKNKVSEALLGFENMGLSVTIRDGKVYVSLEEKLLFKTA
                     SWEIDANGRNALRKLAAVLEKNPDIQITIEGHTDNVPYNPSGGQLRDNWDLSVKRATT
                     VVRVLLESAKIDPKRLTAAGRSQYLPVDPRNTPEARQKNRRTEIILTPDLSELYNLIN
                     KY"
     gene            6162..>6912
                     /locus_tag="PLG42_06200"
     CDS             6162..>6912
                     /locus_tag="PLG42_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOU30833.1"
                     /translation="MTSEYRILSKVSDPSDLRKLRKEDLIVLANELRQFIIDSVSTNP
                     GHLGASLGVVELTIALHYVFNTPYDKIIWDVGHQAYGHKILTGRRDKFHTNRKYKGLS
                     GFPKMSESEYDAFGTGHSSTSISAALGMAIAAERKKENRHIVAVIGDGAMTAGLAFEG
                     MNNAGIQKSNLLVILNDNNISIDPNVGAFKEYLLDITTSRTYNRIKDEIWKALGKISR
                     MGPDARSFAAQLEAGLKSTILDRSSLFEGLNF"
BASE COUNT         2254 a         1352 c         1358 g         1948 t
ORIGIN      
        1 aatgtccatt tgttgagagg gtaagcctta tgcctctgga aagcgaaacg aatttaaaaa
       61 aatctgggtg gagcatcggc tcgccctgaa agtagaggtt aacattcaac aaataagact
      121 ttaattcact taaaattttt ttataaagtt ccacctccat aaatcctgat ggccttttgc
      181 tttgccctga tcccgtgagg cattcagggc atctgaggtt acattttccg gaaatctcca
      241 cattcagagc tgctggcatt ccataaacta cagctctgtg cactagaaga cttgtaaggt
      301 acgatataag tcctccggtt aaattaatta tccttaccat ttgtaactct cgcttgcagg
      361 tttattgtga aggtaaaaaa ttccacagaa aaactattgg aatttttcta tagggtatac
      421 ctatactttg aaggtcttat atatgaaaaa atcaagaaca gaaaaaattc aacacaatta
      481 taaactataa accaaaaact ctgcagccat gaaaaagtta agcatttatc cgggaattgt
      541 tcttacaatt cttcttgttt tatccacggc ttttcctgtt ctggcaggga agccgaaggc
      601 aacaaagaat cccgaccagc aatcaattct ggtactcagg ggtagggtgg tagataaaga
      661 gactcttcag cctcttattt ttgcgtcagt tgcagttaaa gaaacaaacg ttgcaacgat
      721 aaccaatatt gacggtgaat ttattattaa gatacctgaa actgaagttt ctaagaatct
      781 tgaaataacc catattggtt ataaaaacaa ggttattcca ttgatagagt tgaaagatga
      841 cggattcagg aacatcatta caatggagtc tgctcctgtt ccactgaagg aaataatcat
      901 caaaccaatt atgcctgatg aaatcatcag caaacttctc agttcaattt caaagaatta
      961 ccccaacata ccaaatatga tgactgcctt ctacagagaa actataagaa aaaacaggca
     1021 gtacatttct attggtgaag ctcttgtgga aatattcaag gctccatatc agaatgatct
     1081 gaggtttgat gccacacgaa tatataaagg cagaaagaat actgatatgg caaaatctga
     1141 tactgttctc tttaagctgc agggcggacc tgtaaccgca ctggagcttg acctgataaa
     1201 aaatccggag gctattctga ctcctgaggc aataaaatac tacgattacc ggcttactgg
     1261 aatggtggaa atagatgaaa ggcctaacta tatagttgaa ttcagccaga agaaagatat
     1321 cacaatgcca ttgtttatgg gtaaacttta tattgacacc aagactttcg ccctttcggc
     1381 agctgaattc ggacttaacc ttgaaaacaa agaggaagcc acagcattat ttatcaagaa
     1441 aaaacctctt ggcatggaag taactcccga agtagcatca tatatgataa agtaccgcga
     1501 acaggacggg aaatggtact ttgcatattc aagagcagaa gtcagattta aggtcaactg
     1561 gaagaggaaa ctcttcaatc ctcactatac tacaatgtca gaaatagcag tcactgatcg
     1621 cactgaagag gaagttatta aatttgcagg gaaggaaaaa ctaaggtata ctgatatctt
     1681 cctcgatgaa gtttcagctt ttgccgatga gaatttctgg ggtgaatata atataattga
     1741 acccgaccag agtatagaac aggctataag aagactcgcc agaaaagtca aattcagcga
     1801 ccgtccgcct gaagaaaaat aactcatgaa ataatgccgg aagaaaggga aataataagg
     1861 aggataagag aaggtgacat aaaagaattc gaaacacttt tccgatcctc ctatatttct
     1921 cttgtaaaat atgcaggcac acttgttaaa gacagagata cagctgaaga aattgttcag
     1981 gatctttttg tgaaaatatg gaacgaaaga aaaaacctca atattaaaag ttccttaaca
     2041 ggttatcttt tcaggtctgt acataaccgt tgcctccatc atcttcagca tctgaaagta
     2101 gttgaaaaac acgctgagag aatatatgaa acgaacaccg ggatagctga tgaagctgat
     2161 ggagaaatta acctgaagga actcgaagat aaaatagcac gtgtgcttga aaaacttccg
     2221 gaacggtgtt gtaaaatatt ctgcatgaac aggtttgaag gtcttaaata taaagaaata
     2281 gcagaaaaac tttcaatttc tgtcaagaca gtggaagcat gcatgggaaa agcattaaaa
     2341 gaactaagga aaattataga ggattaatta atatgaaacc atctgaaaaa tttacggatc
     2401 agtactggga aaggcttgct gcaaggtttt caggcgaaga aacaaccgga gatgaatttt
     2461 ctggcaatgt cagttctgaa actgaaaaaa tgctcgaaag atactggaaa tctgcaggtt
     2521 cagaaataaa ttccgaggaa ataaacattg agagtgcctg gaacatagtc aggtcaaaaa
     2581 tatccagtga aaaagtccac cctcaccagg ctggggtaag aaaatcaata tccctgtatc
     2641 tcagaatagc tgctgctgtt ttgctccttg cagctctcgg gacagtcact tatgttttat
     2701 ttgataaagc tgtgtcagga agcaggatta ttattgcaac aggtgaagag gaaaagaaca
     2761 gggagattgt ccttcccgat ggcagcaagg tttggctaaa tcatagttcg gaaatctcat
     2821 attcaaaaaa ctggaacagg acgggaagaa aggtgacctt aagcggagaa ggttacttcg
     2881 acatagtccc tgatgaaaaa aatcccttct cagtagatgc tggtaaagca gaaataaagg
     2941 ttgtaggaac ctctttcaat gttattacac gtaaccttga aggagaggtg gaagtgttcg
     3001 tggaaagcgg gaaggttctc cttgccgatg aaaaaggcgg cagcatactg cttgagcctg
     3061 gctatatagg aacaacagct ccgggccgac tcaacaaaac cctgaataat aacagaaatt
     3121 acttgtcatg gaaaacctcc cttctagtat atgaaggaac aagtttgaca gatgttttca
     3181 gggatcttaa aagagcatac aatatagata tctcagttgc tgattcttca atacttgata
     3241 aaactattac ggcagtattc aacaatgacc ctgtagaaac aattattcgc gtaatttgta
     3301 ctacctttaa cttgagttat atcaaggatg gaaaacattt ccacctattg aaaaattaac
     3361 ttaaaggaaa tatgaaaaac tgcaatgtct ggctggtaag aaaaaaaaca ggcattgcat
     3421 tgcttgtttt actctgttgc agcatctggc tgaatggaca ggagaatatt ctcgattcat
     3481 tgtttacttt taagtccgga actgttaagg tcagcaatgc ttttaacatt atcagccggc
     3541 agacgggtta ctcctttacc tacgacagca agctgattaa tcctgacaaa aaagtctctt
     3601 taacctttca gagaacccag ttgagagaaa tacttaatca gataatttca aatgattctg
     3661 ttgattttac ggtaatcaat aaatatataa tcatttacaa aatcactcca taccagccct
     3721 atgctattga aatacctgaa tgggaatata aggatctgac aggaaccatt tcagactttg
     3781 aaaccggtga accaattcca tatgctacaa ttggcataag gtcggtggga cgtggaactg
     3841 ttgcaaacaa taacggagaa ttccttttta agattaaccg cgactggata aaagataccc
     3901 tggtaatatc acacataggc taccagcaaa gaaaaattcc tgtctggcag gctatcggta
     3961 acgagttcaa cataaaatta aaaagggatt acattccgat tccggaaatc ataatccgca
     4021 accagcctcc gcaggagatt ctcagaaaag cttacactta tgtctctcga aactatggca
     4081 acacaccagc aatgcttaca ggtttttaca gggaagctgt catcaaaaaa aatgacctgc
     4141 agatatactc cgaagctatc atcgggatat ataaatcacc ttacacctca tcattgctgg
     4201 gtgaccagat aaagatcttc aaaagcagga aaatggaaaa tatcggcctg aaagatacta
     4261 ttacagtcag gcttaaagca ggtttaaata gttgccttga actcgacgga gctaaaaaca
     4321 cattcgattt cctgtatcct gagaactttt cgcaatatga ttacagaatg actgacattg
     4381 taaaagttga tgaaggaacg gcttttgttg ttgaatttgt tcagaaacca gaagttgaaa
     4441 ttcccctttt tacaggcagt atatatatta atactctcaa ttatgccatc gaatatgcag
     4501 aatttgaagt taatcctgca tatattaaaa agaacaaaga agattttatt acctatcagg
     4561 ccaaaggata cacaattcta cctatatcaa taaaatacag agtaagctat aaaaatatca
     4621 acggtcggta ttttcttaat cacgtaagag gcgacctgaa ttttacagcc cgtcagaaaa
     4681 accatttatt tacaacaaat ttccttgtct tttttgaaat ggccgtaact gatatccgta
     4741 ctgaaaatgt tacaaggttt gaaagggatg aaacagcacc tcttcagtct gtcttttcaa
     4801 aaaccattac aggttatgat tacgatttct ggggcgactt cgattttctc aagcctgaag
     4861 acaaccttct taatgaactc aacaacatga aagcccgttt gcaaatcttc tcaaaggaag
     4921 aaggcaaata acagaattct ttcatcctga tgctgaacct gaagtaatgt taattgaaaa
     4981 agtcagggtg aactactctt tcaatatttg tttatcagat tatacaattc cgacaggtcg
     5041 ggtgtaagta ttatttcggt tcttctgttt ttttgtctgg cttcaggagt atttcgtggg
     5101 tctaccggca ggtactggct gcgacctgcg gcagtaagtc gtttaggatc tattttcgcg
     5161 ctttccagaa gcaccctgac cactgtggtt gccctcttaa cgctcagatc ccagttatcg
     5221 cgaagctgac ctcccgaagg attatatgga acattatcgg tatgtccttc aattgttatt
     5281 tgaatatcag ggtttttctc aagtaccgcc gccagctttc ttaaagcatt ccggccattg
     5341 gcatcaatct cccagctggc agttttgaac agtaactttt cttcaagtga aacataaact
     5401 ttgccgtcgc gaattgttac agacagtccc atattttcaa accccagcaa cgcttctgaa
     5461 actttgtttt tcaactcctg aacaatcctt ttctgagttt caagaatctt ttccagctca
     5521 gccattctgg cattccgctt ttcaagctca tatgaaagct catcaagtga agctttcttc
     5581 atatcaagat tggcttcaag ctgacgaagc aaatcctctt ttttctgaag attctcctgt
     5641 gctgcctgta actccgacaa taatcttctt gtttcagtaa cattgccctt aatcaggtct
     5701 tctctcgcct tttccagatc atttacctta ccctgcagaa tgttatattc ttctttaagc
     5761 ctgtctctct cagttcgcaa agctgtcaac tcgcctgaca tagattttgt ttcttcttcc
     5821 agtttctgaa ctcttgcctc aagttctttc ctttccatgg caagcataat gttttccgtc
     5881 ttcagttcat ctcgctcatt catatacatt ttggcggtgt cctgcattga tttgtattta
     5941 gttccgggta cacatgatga tacagccaga actgatactg ccagaagtat taacccttta
     6001 atttttgaca ttacacctta tttttaaagt taataagtga tacttttccc tgtgtgcagt
     6061 ttaaacaaac gtatgaacaa aaatataaaa aacctgtttg atgcataaac aggcacagaa
     6121 ataattaact atctttgtgc tgtctatacc cagaacgctt gatgacttca gaataccgta
     6181 tcctgtcgaa agtttcggat ccctcagatt tacggaaact caggaaggag gatcttattg
     6241 ttcttgctaa cgaactccgg caatttataa ttgattcggt aagtactaat ccgggtcatc
     6301 tgggtgcaag tctgggtgtg gttgagctaa caattgccct gcattatgta ttcaatactc
     6361 cctacgataa aattatctgg gatgtagggc atcaggccta tgggcacaag atacttacag
     6421 gccggcgcga caaattccat acaaaccgta agtataaagg actgagcggt tttccgaaga
     6481 tgtcagaaag tgaatacgat gcatttggca cagggcattc ctccacttct atttcagctg
     6541 ctcttggtat ggctatagca gcggaaagaa aaaaagaaaa caggcacatt gtggcagtta
     6601 ttggcgatgg agccatgact gccggtctgg cttttgaagg aatgaacaat gccggaatcc
     6661 agaaatcgaa ccttcttgtt attttgaatg ataacaacat atccattgat ccaaatgtag
     6721 gagcatttaa agagtacctt cttgatataa ccaccagccg tacatacaac aggatcaagg
     6781 acgagatatg gaaagctctc ggcaaaatca gcaggatggg gcccgatgcc aggtcgtttg
     6841 cagcccagct tgaagctggt ctgaagagta ccatactcga ccgcagcagt ttgtttgaag
     6901 ggttgaattt ca
//
LOCUS       DAOSNB010000124         6906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11034_length_6906_cov_3.452489, whole genome shotgun sequence.
ACCESSION   DAOSNB010000124 DAOSNB010000000
VERSION     DAOSNB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11034_length_6906_cov_3.452489"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLG42_06205"
     CDS             complement(<1..638)
                     /locus_tag="PLG42_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOU30834.1"
                     /translation="MRKKILMLSVFLMIIVFINGVYVYAGEVSSNKRPVNIILFIGDG
                     MGPAQVSAAIAVNDNTLVVEKFKYAGYCKTSSYDRYVTDSAAAATAIACGVKTRNGMI
                     GMGPDSTAVMSILEIAKKNGLATGVVSTSAVTHATPAGFVAHNSGRGNYEDIALDFLK
                     GTADVFIGGGSNHFVKRKDGKDLSANLKKMGYDVVFTLEDLIKSNSMKIAGLL"
     gene            complement(696..2453)
                     /locus_tag="PLG42_06210"
     CDS             complement(696..2453)
                     /locus_tag="PLG42_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOU30835.1"
                     /translation="MSGNKIIKVICCYLLIICAVSCGQEAGKRIVILATTDLHGTIFP
                     YDYIEDKPLKASLSNISTLVSDVRKKYNTILLDNGDNLQGQPAVYYYNYIDTLSRHIN
                     SMVMNYMGYDAGTVGNHDIEAGHSVYDRLRNEYNFPLLAANAVNIKDGEPYFKPYIIL
                     EKYGLKIAVFGLITPSVPEWLPPVLYSGMEFRDMIETAKKWMPQILERKPDIVVGLFH
                     SGTGMSENRPGEFGKDYENSSGAVAYYVPGFNIVIAGHDHNELCSEIINVNGDTVLIL
                     DAGSRGEKVARADIEIKGITGKKRIKISGSLIDVSNYNPDKNFIDEFSEKEKKIKEFV
                     KEVIGESSASFSSREAFFGPSAFVDLIHKIQLEITGADVSFAAPLSFDVKIDKGPLTV
                     GDMFKLYRYENMLYTMELKGYEIVKYLEYSYAEWLNTMKSPDDHLLNFRTDKDGKIYM
                     SGGKAYLKYPSYNFDSAAGIEYVVDVSKPAGKRIKIISMQNGKSFYDNNTYLVAVNSY
                     RGSGGGGHLTEGVSLNRVEIAERLKYSTVRDLRYYIIEYIRDKKMIHPESFDSWKIIP
                     EQWVEKARKKDYKLLFGEQ"
     gene            complement(2502..5300)
                     /locus_tag="PLG42_06215"
     CDS             complement(2502..5300)
                     /locus_tag="PLG42_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU30836.1"
                     /translation="MIFLFITVVITLNAQKIPLSHDVYDTWYSISNRIVSEDGKWITW
                     EKNPQQGDSWLYVYNTDKRETDSVFCGYRASFSPGCKYLVYMIKPTYSETRQAKVKKL
                     KQDQMPKSNMVIRVLDTGKRYNFEKVKSYSLPESGSLWMAFLFEKKNENKSPEKSNNE
                     NQGEKQKQASSGKQAEAKGTELVILNPVTGKEFRYNDVTEYVVARDGRSISFLQDIQD
                     TTKIDTYKVFQFNTEKETLSLLFEGKGSLEKLSCDRSGELLAFMYTSDTSKIKVFDLF
                     LSKSGNSAVKIPLQGLPDGWVLSRDTTVSFSEDRTRLFFGTAPAPVKEPEDTLLDEEK
                     YKLDIWSWNDDVLQPMQKKQLNREKKRVWLAVYHIDRNRMIQLATKDMPDIVLLQKGN
                     NDIALGSSNIKYRKLISWDANNYADYFIVNVETGQKSLILEKAPSRVIISPSGKYVLY
                     WNIDEKQWISYSVASGTKKAITTDIPYPLYDEISDIPSPPSPYGIAGWMDDERYVLVY
                     DRYDIWKADLTGTEKPVCLTNSFGRSKNLTFRYVRLDRDAEFINRKELLYLSAFDNTT
                     KESGFFTAKFPGPQEPVKLVMGKCSFQGGLTKAKKADILLWQKGSFEEYPELYFSDMK
                     FSEPVKISITNPQQSKYIWGSVELVSWKSFDGKELQGLLYKPENFNPAGKYPMIVYFY
                     ERSSDGMYSYIAPAPSASTINRTFAVSNGYLVFVPDIVYNIGYPGQSCYDAVVSGVQS
                     LVERYSFIDREHIGLDGQSWGGYQVAYLVTRTNMFACAFAGAPVVNMISAYGGIRWAT
                     GMSRMFQYEMTQSRIGGTLWEKPLHFIENSPVFRIPEINTPLLIMHNDNDGAVPWYQG
                     IEFITALRRLGKPAWMLSYNDEEHNLEKRPNRKDLSIRKMQFFDHYLKGAPMPYWMKN
                     GISQLEKGKKDGYELIYN"
     gene            5420..6595
                     /locus_tag="PLG42_06220"
     CDS             5420..6595
                     /locus_tag="PLG42_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU30837.1"
                     /translation="MVKTGLQVFLEKFPAEFRGKRAGVLCHAPSIDKDFVHITDLLYN
                     SGQCKLTAIFGPQHGLFGQTQDNMIEWEGYTHPVYKIPVFSLYGKLRKPDKKMLERID
                     FLIVDLQDVGARLYTYAWTMKLCMEACSEMGIPVVVLDRPNPVAALDFDGPVLKEEYF
                     TFVGGASIPLCHRMTIGEIALWIKEKHIKNCKLQVIKMVNWRRSMMYNQTGLPWVLPS
                     PNMPTLNTAVVYPGMVLFEAMNISEGRGTTIPFELFGAPFIDPYRLINHLREKNIQGC
                     KFRIHCFIPTFNKYSGIFCNGLQIHVTDIKKFKPVVAAMEIIDSIIRTSEDGTVKFND
                     PPYEYEEKLIPFDILAGDSLMRETLEKGKPTSMETERWQAEIEEFKKDFRNIALYSE"
     gene            complement(6616..>6906)
                     /locus_tag="PLG42_06225"
     CDS             complement(6616..>6906)
                     /locus_tag="PLG42_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOU30838.1"
                     /translation="QTGLYLPPGVNENEKVQVGYVLKVGPGYPIPTIVDNDEPWKNKS
                     NEPKYVPLQARVGDQAVYMQSQAIEIEFNKEKYIVVPQSAILLLVRDEGLFE"
BASE COUNT         2038 a         1458 c         1199 g         2211 t
ORIGIN      
        1 agaagccctg caatcttcat tgaatttgat ttaatcaggt cttcaagtgt aaagacaacg
       61 tcataaccca ttttcttcaa attggctgaa aggtcttttc catcctttct cttaacaaaa
      121 tgattagaac cacctccaat gaatacatca gctgtacctt ttaagaaatc aagggctata
      181 tcctcataat tacctcttcc tgaattatga gcaacaaagc cagccggagt agcgtgagta
      241 actgcacttg tactaaccac accagtagcc agtccgtttt tcttggcgat ttcaagaata
      301 gacattacag cagttgaatc aggacccatt cctatcattc catttctcgt tttaaccccg
      361 catgcaatgg ctgttgcagc agctgctgaa tctgttacat accgatcgta tgaagaagtt
      421 ttgcaataac cagcatattt aaacttctca acaacgagtg tgttatcatt tacagcaatc
      481 gcagcagaaa cttgcgcagg acccatacca tcgccaataa acaaaattat gtttacgggt
      541 cttttatttg aacttacttc accggcgtaa acatatactc cattgataaa tacaattatc
      601 atcagaaaaa ccgatagcat caaaatcttc tttctcatat cttcataatt ttaatgaaac
      661 aaaactattt atattactaa actattttgt atacgtcatt gttcgccaaa taataatttg
      721 taatcttttt tcctggcctt ttcgacccat tgctcaggta tgattttcca cgaatcaaat
      781 gattcaggat gtatcatttt cttatccctg atatattcta taatataata cctcaaatcc
      841 cttaccgttg aatatttgag tctttccgca atttcaaccc tattcaggga aacaccttcg
      901 gtaagatgtc ctcctcctcc acttccccgg taagaattaa ctgccacaag gtatgtatta
      961 ttatcataaa acgatttccc attttgcata gaaattattt taattctttt gcctgcaggt
     1021 ttgcttacat ccacaacata ttcaatacca gcagcagaat caaagttata tgacggatac
     1081 ttgagatagg ctttccctcc actcatatat atttttccat ccttatcagt tctgaagttc
     1141 aacaaatgat catcagggct tttcattgta ttaagccatt ctgcatatga gtattcaagg
     1201 tactttacta tctcataacc ttttaactcc attgtataaa gcatgttctc atacctgtat
     1261 agtttaaaca tatctcctac agtaagaggt cctttgtcta tctttacatc aaaagaaagg
     1321 ggagctgcaa aagagacatc agccccggtg atttcaagct ggattttatg tatcagatcc
     1381 acaaaagctg acggaccaaa aaaggcttct ctcgatgaaa aagaagcaga cgattcaccg
     1441 ataacttctt ttacaaattc ttttattttt ttctcttttt ctgaaaattc atctataaag
     1501 ttcttatcag gattgtaatt gcttacatca attaatgaac cagatatttt gattcttttt
     1561 ttcccggtaa ttcctttaat ttcaatatct gctctcgcaa ccttttcccc cctgctgcca
     1621 gcatcaagaa taagtactgt atctccgttt acatttataa tttcgctaca taattcattg
     1681 tgatcatgac ctgctataac tatattgaag ccgggaacat aataagcaac tgcacctgaa
     1741 ctgttctcat aatcttttcc aaactcacct ggtctgtttt cactcatacc cgtacctgaa
     1801 tgaaaaagac ctacaacaat atcaggcttc cgctcaagaa tttgtggcat ccattttttt
     1861 gccgtctcta tcatatctct aaactccatt cctgaataca aaacaggagg taaccattct
     1921 ggtactgaag gagttattaa cccgaaaaca gcaatcttca atccatattt ttccagaata
     1981 atataaggtt taaaatatgg ctctccgtct ttaatattta cggcatttgc agcaagcagc
     2041 gggaaattat actcatttct gagccggtca taaacagagt gtcctgcctc aatatcatga
     2101 ttacccacag tacctgcgtc atagcccata taattcataa ccatgctatt gatgtgacgg
     2161 gaaagagtat cgatgtagtt atagtaatat actgcaggct gcccctgtag attgtctcca
     2221 ttatcgagca atattgtatt gtattttttc ctgacatctg aaacaagggt tgaaatattt
     2281 gataacgagg cttttaaggg tttatcttct atataatcat agggaaagat agttccgtga
     2341 agatctgttg tagcaagaat aactattctt ttacctgcct cctgcccgca tgaaacagca
     2401 catataatca gcaggtaaca gcagattact tttatgattt tatttcctga cattatataa
     2461 taatttatca atcttgctat aaatttgaag cttttaaatt tttagttata aataagctca
     2521 taaccgtctt ttttaccctt ttcaagctgg gatattccat ttttcatcca gtaaggcatt
     2581 ggagcgccct tcagataatg atcaaaaaac tgcatctttc tgattgacaa gtcttttcgg
     2641 tttggtcgct tttcgaggtt atgttcctcg tcgttatatg acagcatcca tgcaggttta
     2701 cccagtcgtc taagtgcagt aataaattct atgccctgat accatggcac ggcaccgtcg
     2761 ttatcgttat gcataattaa taatggagta ttaatttcag gaatcctgaa aacaggtgaa
     2821 ttttcaataa aatggagtgg tttttcccag agtgttccgc ctatcctgct ctgagtcatt
     2881 tcgtactgga acattcttga cattcctgta gcccatctaa tgcctccgta tgcgcttatc
     2941 atgttaacca ctggcgctcc ggcaaaagca caggcaaaca tatttgtacg tgttaccagg
     3001 taggcgacct ggtaacctcc ccaactctgt ccatcgagac caatatgttc cctgtcgata
     3061 aaactgtatc tttcaaccag cgactgtaca ccacttacaa cagcatcata gcagctctgc
     3121 ccgggataac cgatattgta aacaatgtca ggcacaaaaa ccaggtaacc gttgcttact
     3181 gcgaaagtac ggtttatggt tgaggcactc ggagctggtg ctatatagct gtacatccca
     3241 tcggagcttc tttcatagaa ataaacaatc atcggatatt tgccggcagg gttaaaattt
     3301 tcaggtttat ataacagacc ctgaagctcc ttgccatcaa acgacttcca cgacacaagc
     3361 tcaacactcc cccatatata ttttgattgc tgaggattgg ttattgaaat ttttacaggt
     3421 tctgaaaatt tcatatcact gaaatatagt tcaggatatt cttcaaaaga acctttctgc
     3481 catagaagaa tatcagcctt ctttgcttta gtcaatccac cctgaaaaga gcactttccc
     3541 attacaagct ttaccggttc ttgtggtccg ggaaactttg cggtaaaaaa tcctgattct
     3601 tttgttgtat tgtcaaatgc tgaaagataa agaagctcct tcctgtttat aaactctgca
     3661 tccctgtcga gtcgcacata acgaaaagtc aggttttttg atctgccgaa gctgtttgtt
     3721 aaacagacag gcttttctgt accggtaaga tctgctttcc agatgtcgta acggtcatac
     3781 acaagaacat atctttcatc atccatccag ccagctatgc catacggtga tggtggtgaa
     3841 ggaatatctg atatttcatc atataatgga tagggtatgt cggttgttat tgcttttttt
     3901 gttcccgatg caactgaata cgaaatccat tgtttttcat caatattcca gtaaagcaca
     3961 tattttcccg acggagaaat tattactctc gagggggcct tttcaagaat gagcgatttc
     4021 tgtccagttt caacatttac aataaaataa tcagcataat tattggcatc ccaggaaatc
     4081 agtttacggt atttgatatt tgaagagcca agtgcaatat cgttatttcc tttttgaagc
     4141 aatacaatgt cgggcatatc cttcgttgca agttgtatca ttctgtttct gtcaatgtga
     4201 tatactgcaa gccacactct ttttttctcc cggttaagtt gctttttctg cataggctgg
     4261 agaacatcat cgttccatga ccatatatcc agtttatatt tttcttcgtc aagcagagta
     4321 tcttccggct cttttaccgg ggctggtgca gtaccaaaaa ataatcttgt gcggtcttca
     4381 gagaaagaaa cagttgtgtc tctgcttaac acccagccat ctggtaaacc ttgcaacggt
     4441 atctttacag cactgttccc tgattttgaa agaaataagt caaaaacctt aatctttgaa
     4501 gtatctgatg tgtacatgaa tgcaagcagt tccccggatc tgtcgcatga aagtttttcc
     4561 agagagccct taccttcgaa tagaagggat aaagtctcct tttcagtgtt aaactgaaaa
     4621 accttataag tatctatttt tgttgtatcc tggatatcct gcaaaaagct gattgaacgt
     4681 ccatcgcgtg caacgacata ttctgtcaca tcattgtacc tgaactcctt gccagtcaca
     4741 ggatttagaa ttacaagctc tgttcctttc gcttcagcct gtttaccgga agatgcctgt
     4801 ttctgtttct ctccctgatt ttcattattt gatttttcag gtgatttgtt ttcatttttc
     4861 ttctcaaaca aaaatgccat ccatagagat cctgactcag gaagagaata tgatttaact
     4921 ttttcgaaat tatacctttt gccggtatcc agaactctga ttaccatatt gctcttcggc
     4981 atctgatcct gttttagctt tttaaccttt gcctgccgtg tttcggaata agtaggttta
     5041 atcatgtaga ccagatactt gcatccgggc gaaaaagaag ctctgtaacc gcaaaatacc
     5101 gaatcagttt ctcttttatc cgtattatag acataaagcc agctatcacc ctgctgagga
     5161 ttcttttccc atgtaatcca tttaccgtct tctgacacta tgcgattgga aattgaatac
     5221 caggtgtcat aaacatcgtg actcaatgga attttctggg cattcagggt gattaccacc
     5281 gtaataaaca aaaaaatcat taaatttttg agtagcattc tattcttatt tttaattgac
     5341 ctaaacatat aaaagaattt tcaacctgca tagttaaaaa attcaaatat gaattatttt
     5401 gcagcctaaa ataaaatata tggtcaaaac tggtttgcag gtttttcttg agaaatttcc
     5461 ggcagaattt cgcgggaaga gagccggagt tttatgtcat gctccaagta tagataaaga
     5521 ttttgtgcac ataacagacc ttctttacaa tagtggccag tgcaaactta cagcaatttt
     5581 tggtccacag catggtttat tcggtcagac gcaggataat atgatagaat gggaaggata
     5641 tactcaccca gtttataaaa ttcctgtttt cagtttgtac gggaagcttc gcaagccaga
     5701 caaaaaaatg ctggaaagaa ttgattttct tattgtcgac ctgcaggatg tcggtgccag
     5761 gttatatact tatgcttgga caatgaagct ttgtatggaa gcctgctctg aaatgggcat
     5821 accggttgtt gtgcttgaca gacccaatcc tgttgctgct ctggattttg atggtcctgt
     5881 tctaaaagaa gagtatttca ccttcgttgg aggtgcctca atacctcttt gccacagaat
     5941 gacaattgga gaaatagcat tatggataaa ggagaaacat attaaaaact gtaaattgca
     6001 ggttataaaa atggtaaact ggagaaggtc gatgatgtac aatcaaaccg ggttgccatg
     6061 ggtattgcca tctccaaata tgccaaccct gaacactgcc gttgtttacc cgggtatggt
     6121 actcttcgaa gctatgaata tatctgaagg aagaggcaca acaatacctt tcgaactgtt
     6181 cggtgcgcca tttattgatc cctacaggtt aatcaaccat cttagggaga aaaacattca
     6241 gggttgcaaa ttccgaattc actgtttcat accaactttt aacaaatact caggcatctt
     6301 ttgcaacgga cttcaaatac atgtgacaga tattaaaaaa ttcaaacctg tggtcgcagc
     6361 gatggaaata attgattcca ttatcagaac cagtgaagat ggtacagtaa aattcaatga
     6421 tcctccttat gaatatgaag aaaagctaat accctttgat attctggctg gtgattcact
     6481 tatgagggag acacttgaaa aaggcaaacc aacttccatg gaaacagaac ggtggcaggc
     6541 ggaaatagaa gaatttaaaa aagacttcag aaatatagcg ctgtacagtg aataaaagct
     6601 ctggtaaaac aatatctatt caaaaagtcc ctcatccctt accagaagta atatagctga
     6661 ctgaggcacg acaatatatt tctccttatt gaattcaatc tcaatagcct ggctttgcat
     6721 gtatactgcc tggtctccta ccctggcctg aagcgggaca tattttggtt cgttcgattt
     6781 gtttttccat ggctcatcgt tgtcaacaat agttggaata ggataacccg gcccaacttt
     6841 caaaacgtac cccacctgaa ccttctcgtt ttcattgact cctggaggaa ggtaaagtcc
     6901 ggtctg
//
LOCUS       DAOSNB010000125         6904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11036_length_6904_cov_4.009782, whole genome shotgun sequence.
ACCESSION   DAOSNB010000125 DAOSNB010000000
VERSION     DAOSNB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11036_length_6904_cov_4.009782"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLG42_06230"
     CDS             complement(<1..289)
                     /locus_tag="PLG42_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU30839.1"
                     /translation="MPRILVIDDEKSIRNTLKEVLEYENHEVDLAEDGPSGIELFSNN
                     AYDIVLCDIKMAKMDGIEVLEKILEISPDTPVIMISGHGNIDTAVESIKKGA"
     gene            complement(319..1683)
                     /locus_tag="PLG42_06235"
     CDS             complement(319..1683)
                     /locus_tag="PLG42_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU30840.1"
                     /translation="MSKISVIIEREYITRVRKKSFIIMTILAPVLLALFIVVPSLIMM
                     NQDTEMKKIAVVEDGTDLFKNAIPNIKNTEFVYLGNVKLEEIKNNFEQAGYYGILYIS
                     PEVIHVPEAVQLISRKQPPILLLEHIENSLEKEIERQKLLSYKIENLDEIMKNVRTRV
                     SVQTIKIDESGNVKETSTGVAMVLAYITSFVMYFLVFVFGAQVMRGVIEEKTSRVVEV
                     IISSVKPIQLMMGKIIGLALVGLTQFLIWIILTFAIITAVKSALIPEQKITEAVQSIP
                     QDIMSQNAAAAMQTTTVTTTEPVQASEFIKIFNSALNQPWGLIIFCFIYYFITGYLLY
                     ASVFAAVGSAVDNETETQQFMLPITLPVILGLFVAMSTMQNPESSMAFWFSIIPFTSP
                     IVMMARIPFGVPLWEIALSMFLMAVTFIIFVWMSAKIYRTGILMYGKKSSWKEIWKWL
                     TYKG"
     gene            complement(1704..2627)
                     /locus_tag="PLG42_06240"
     CDS             complement(1704..2627)
                     /locus_tag="PLG42_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU30841.1"
                     /translation="MIIFEARNVSKQYGSTLALNNLSISVPKQSIYGLLGPNGAGKTT
                     LIRIINQITGPDSGELLLEGRKLRQDDIYEIGYLPEERGLYKKMKVGEQALYFARLKG
                     ISKAEAMKRLRYWFKKLEMTDWWNKKVEELSKGMAQKVQFVTTVLHEPKLIILDEPFT
                     GFDPVNASIIKNEILFLRDRGATILLSTHDMASVEELCDNITLIDHGMSMLEGKVDEI
                     RRAWAGNEYSVTFEGNIRVPENGNFQILHTDNEDNKTTVKIKCLKDISTNEILSELMK
                     SGNIIAFNPALPSMNDIFIRVVKSKSGMPVN"
     gene            complement(2807..3952)
                     /gene="dnaJ"
                     /locus_tag="PLG42_06245"
     CDS             complement(2807..3952)
                     /gene="dnaJ"
                     /locus_tag="PLG42_06245"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOU30842.1"
                     /translation="MTKKDYYEILGVPRNASKEEIKKAYRKLALKYHPDRNPGNKEAE
                     EKFKEAAEAYEVLSDDEKRRIYDQYGHAGLGAPGSGFSGGMTIEDIFASFGDIFEDAF
                     GFSGFGTSSRRSRRVNKGTNLRVKVKLDLNEIAYGAEKKIKVRKYVTCPACDGTGAAD
                     SRSYSDCPTCRGTGHVTRITSTFLGQMQTTSTCPSCGGEGKIITRKCSSCYGEGIVEK
                     EEIISINIPAGVSKGMQLTLSGKGNAARRGGIPGDLYVLIEEEPHPELIREGNDLIYN
                     LFISIPDAITGCYAEIPTIDGQVKIKIEPGTQPGKILRLRGKGLPDVNGYGRGDLLVN
                     VNVWIPKNLSREDMKIVEKLKESGAFTPRPDKDDKGFFERMRSYFNK"
     gene            complement(3965..4630)
                     /locus_tag="PLG42_06250"
     CDS             complement(3965..4630)
                     /locus_tag="PLG42_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOU30843.1"
                     /translation="MTKRKHTEGKKETEELKEKTTKNANNTEEEKIEMNEQPAEEAVE
                     TTVEQPVAESAAMPEKQEGEPVQVEESISREAELLDEVAKWKDDYIRLMAEFDNYRKR
                     TLKEKIDLTKSAGENILKGLLPIVDDFERALKALENSADSSNIKEGIELIYNKFIEFL
                     KQQGLREIEALNCEFNVDLHEAVNKVAVEDESLKGKVIEVLQKGYYLNDKVIRFSKVV
                     VGE"
     gene            complement(4752..6059)
                     /gene="murA"
                     /locus_tag="PLG42_06255"
     CDS             complement(4752..6059)
                     /gene="murA"
                     /locus_tag="PLG42_06255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOU30844.1"
                     /translation="MSSFIVHGGRSLKGEITPQGAKNEALQVICASMLTSHKVTIQNI
                     PRILDVENLIDLVSSLGVKVTKESSGSTVTFEATSLNPDNLKDPGLIEKGQRIRGSIM
                     IAGPLLARCGKVFVPTPGGDKIGRRRLDTHFTGLIKLGATVSHNNADNYYTLEAKRLK
                     GNYILLDEASVTGTANIIMAAVLAEGKTTIYNAACEPYIQQLCRMLVRMGAKIDGIGS
                     NLLTIEGVKELQGCTHTLLPDMIETGSFIGMAVMTESELTIKNVSWDNLGIIPDSFRK
                     LGITLERRGDDIFIPRHEHYRIESFMDGSIMTISDAIWPGLTPDLLSVFLVTATQADG
                     AVLIHQKMFESRLFFVDRLIDMGAQIILCDPHRATVIGLNRKVRLRPTVMTSPDIRAG
                     IALLIAAMSASGTSVIHNVDQIDRGYENIDTRLNALGADIRRI"
     gene            complement(6059..6703)
                     /locus_tag="PLG42_06260"
     CDS             complement(6059..6703)
                     /locus_tag="PLG42_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOU30845.1"
                     /translation="MGFDYNTQRKRMVLPEYGRNIQKMVDYLKTIKDRDERNRAAATI
                     IHIMGNLNPQLRDVEDFKHKLWDHLALIADCDLDIDSPYPPPEKSKLAEKPNRIPYPQ
                     GNIRFLHYGKNIEMMIDAVSKMPDGEEKDYLTYLIINQMKKLYITWNRPQVSDDIILS
                     DLKYLSGGKLTIPEGMKIPEAKELMAQNKKKQQTNKHNKQQNKHSQKKKGFNRH"
BASE COUNT         1700 a         1527 c         1386 g         2291 t
ORIGIN      
        1 atgccccttt tttaatcgat tcaacggcag tgtcgatgtt tccatggccc gatatcatta
       61 taaccggtgt gtcgggcgaa atttcaagta tcttttccag tacttcaatg ccgtccattt
      121 ttgccatttt gatatcgcac agaacgatat cgtaggcatt atttgaaaaa agctctatgc
      181 ccgacggtcc atcttctgcc aggtcaacct cgtgattctc gtattccaga acttccttca
      241 gcgtgttccg gatagacttt tcatcatcga taactagaat tctgggcatc tggcttcaat
      301 gcttgtttta atgagaaatt atcctttgta agtcagccat ttccatatct ctttccagga
      361 agatttttta ccgtacataa gaataccggt acggtaaatc tttgctgaca tccatacaaa
      421 gataataaaa gtgactgcca taaggaacat tgaaagggca atttcccaca ggggaacacc
      481 aaaaggtatt cttgccatca taactatcgg agaggtgaaa ggtataattg aaaaccagaa
      541 cgccattgag ctttcagggt tctgcattgt actcatggcg acaaacagac cgagtatcac
      601 aggcagtgtt ataggaagca tgaactgctg cgtctctgtc tcattatcaa ctgcagaacc
      661 caccgctgca aaaaccgaag catacagaag ataacctgta atgaaatagt aaataaagca
      721 gaatattatt agcccccatg gctggttgag tgccgaattg aatattttaa taaactcaga
      781 tgcctgtact ggttctgtag tagtgactgt tgtggtttgc attgctgcgg cagcattttg
      841 cgacatgatg tcctgcggta tgctttgaac cgcttccgta attttttgtt cgggtatcaa
      901 tgccgatttt accgcagtta ttattgcaaa agttagaatt atccatatca ggaactgtgt
      961 aaggcctaca agcgccaggc caataatttt acccatcatt agctgtatgg gtttcactga
     1021 tgaaataata acctcaacca ccctgctggt tttttcttca attacacccc gcattacctg
     1081 agcaccaaaa acaaatacaa ggaaatacat aacaaaactg gtaatatatg ccagtaccat
     1141 tgcaactccg gtactcgttt ctttaacatt cccgctttca tcaatcttta tcgtctgaac
     1201 cgatactctg gttctgacat ttttcataat ctcatcgaga ttttcaattt tatatgagag
     1261 cagtttctgg cgttcaatct ctttttcaag ggaattttca atatgctcga gtagcagaat
     1321 aggaggttgt tttcttgaga taagctgtac tgcttctggt acatgaatta cttcaggtga
     1381 gatatagagt ataccgtaat atcctgcctg ttcaaaattg tttttgatct cttccagttt
     1441 gacgtttcca aggtatacaa actcggtatt cttgatattg ggaattgcat tcttgaacag
     1501 gtctgtcccg tcttctacta ctgctatttt tttcatttcg gtgtcctggt tcatcataat
     1561 aagcgacggg acaacaatga aaagtgccag caatacagga gccagtatgg tcatgataat
     1621 gaacgacttc tttctgaccc ttgtaatata ttcacgctca atgataactg atattttgct
     1681 cataactttc attttatctg tggttagttg acaggcattc cgcttttgga ttttactact
     1741 cttataaaaa tatcattcat agagggcaga gccgggttga aggcaattat attacctgat
     1801 ttcataagtt cggataggat ctcatttgtt gagatgtctt tcaggcattt aatttttaca
     1861 gtggtcttat tgtcttcatt atcggtatgc aatatttgaa aattaccatt ttcaggcact
     1921 ctgatattac cctcgaaggt tactgagtat tcattacctg cccatgcccg gcgtatttca
     1981 tccacctttc cttccagcat tgacattcca tggtcgataa gagttatatt gtcgcaaagt
     2041 tcttcaaccg atgccatatc gtgtgtggag agaagtattg ttgcaccccg atccctgaga
     2101 aagagaattt cattcttgat gatactggca tttaccgggt cgaagcctgt aaaaggttca
     2161 tcgaggatga taagttttgg ctcgtgaagt acggttgtga caaattgaac tttctgtgcc
     2221 attcctttcg aaagttcttc aaccttttta ttccaccagt cggtcatttc gagttttttg
     2281 aaccagtacc ggagtctttt catagcttct gctttcgaaa tgcctttgag tcttgcgaag
     2341 taaagtgcct gttcacccac tttcattttt ttgtaaagcc ccctctcttc agggaggtat
     2401 ccgatttcat aaatatcgtc ctgcctcagt ttccttcctt caagcaggag ctcgcccgaa
     2461 tcaggcccgg ttatctggtt aatgatgcgg ataagagtcg tttttccggc accgttcgga
     2521 cccagcaggc cataaatgga ttgcttcggg accgagatgc tcagattgtt cagtgccaat
     2581 gtactcccgt attgctttga tacatttctt gcttcgaaaa ttatcataga tcctaaaaat
     2641 ttgttttaaa agggaggtta aatatagtta aatatgaaaa acaaaacttt tcctttgttt
     2701 taaatagtct ccggttttct gaattgattt aaacagtttt gccgtgatac attaatgact
     2761 tcagaagttg ttccggtgct gcatcttacc ggaaaatttt acggggttat ttattgaaat
     2821 aacttctcat tctttcgaag aatcccttat cgtctttgtc ggggcgtggg gtgaaagcgc
     2881 ctgattcttt gagtttttcg acaattttca tgtcttccct gctgaggttt ttgggtatcc
     2941 agacgttgac attcacaagc aggtctcctc gtccgtatcc gtttacatcg ggcagacctt
     3001 tgccacgcag gcggagtatt ttccctggct gggttccggg ttctattttt atttttacct
     3061 gaccgtcaat tgttggtatt tcggcatagc agccggttat agcatcgggg atgcttatga
     3121 aaaggttgta tatcaagtcg ttaccttccc ttatcagttc agggtggggt tcttcctcga
     3181 taagtacata gaggtcgccc ggtattccgc ctctgcgggc tgcattgccc ttgccgctga
     3241 gggtaagctg catacctttg gatactcctg ctggaatatt tatgctaatg atttcttctt
     3301 tttcgacaat gccttctccg taacaggagg agcattttct ggttattatt ttgccttcac
     3361 cgccgcatga agggcaggtg gaggttgtct gcatctggcc aaggaatgtg ctggttattc
     3421 ttgttacgtg tccggtaccc ctgcatgtcg ggcagtcgga atagcttctt gaatctgcag
     3481 cacctgtgcc atcacaggca gggcaggtga catattttct tacttttatc tttttttcgg
     3541 cgccgtaggc tatttcgtta aggtcgagtt ttaccttcac cctgaggttg gtccctttat
     3601 taacgcgtct cgacctcctg ctgctggttc cgaaaccgct aaagccaaaa gcatcttcaa
     3661 agatgtcacc aaaggatgca aaaatatctt caatagtcat ccctccgctg aagccgctac
     3721 ccggagcacc cagaccggca tggccgtact ggtcgtaaat ccttcttttc tcatcatccg
     3781 acaatacctc ataggcttct gcagcctctt taaatttttc ctctgcctct ttattgcccg
     3841 ggttacggtc gggatgatac ttcagcgcaa gcttcctgta ggcctttttt atttcctctt
     3901 tggaagcatt tcgcggaaca ccaagtattt cgtagtaatc tttttttgtc atccttacct
     3961 gatactattc acccacaaca acttttgaga accttattac tttatcgtta agataatagc
     4021 ctttttgaag aacttcaatg actttccctt tcagcgattc atcctcaact gctactttgt
     4081 ttactgcctc atgaaggtcg acgttgaatt cacagttgag ggcttcaatt tctctgagcc
     4141 cctgctgctt gagaaattca ataaatttgt tatatatgag ttcaataccc tctttaatat
     4201 tactgctgtc ggctgaattt tccagagctt ttaatgctct ttcgaagtcg tcaacaatcg
     4261 gcaaaagtcc cttaaggatg ttttctcccg cactttttgt caggtctatt ttttccttaa
     4321 gggttctctt gcggtaattg tcgaactctg ccatcagccg tatgtaatca tctttccatt
     4381 ttgcaacctc atcgagaagc tctgcctccc ttgatattga ttcctcaact tgtacaggtt
     4441 ctccttcctg tttttcaggc atggcagcag attcagccac aggttgttca acagtggtct
     4501 caaccgcttc ttcagcaggt tgttcattca tttcaatttt ttcttcttca gtattattgg
     4561 cgttctttgt tgttttttct ttgagttctt cagtctcctt tttcccttca gtatgctttc
     4621 tctttgtcat ttctcaaagg tttaaaaata tgttgggttt ttaataaacg tatttcatca
     4681 aattacctgc caattgcaaa ttattgccaa tttgacagta aagaaagtta ataaagataa
     4741 aattattttt ttcagattct ccggatgtca gcacccagag cattaagacg ggtatcgata
     4801 ttttcgtagc ccctgtcaat ctggtcgaca ttatgaatca cacttgtacc gctggccgac
     4861 atggcagcaa tgagcagggc aataccggct cttatgtcag gtgatgtcat cactgtaggc
     4921 cgcagtctga cttttctgtt gagtcctata accgttgccc ggtgcggatc gcatagtatt
     4981 atctgagctc ccatatctat caggcggtca acaaaaaaca acctgctttc aaacattttc
     5041 tggtgtatca gcacagctcc gtcggcctgc gtggccgtta ccagaaaaac actgagcagg
     5101 tcgggtgtca gccccggcca gatagcatcc gaaatggtca taattgaacc gtccatgaaa
     5161 gattctatcc tgtaatgttc atgacggggt atgaatatat cgtcgcctct tctctcgagt
     5221 gttattccga gcttgcggaa tgaatcggga attattccaa gattatccca ggatacattt
     5281 tttattgtca gttccgattc tgtcattact gccatgccga tgaaagaacc cgtttcaatc
     5341 atgtcgggca ggagggtgtg agtgcagccc tgtagctcct taactccttc aatagtaagg
     5401 agattggagc ctatcccatc aatttttgca cccattctca ccagcattct gcacagctgc
     5461 tggatatagg gttcgcaggc tgcattataa attgtggtct ttccttcagc cagtacggct
     5521 gccatgataa tgttggcagt tcccgtcacg gaagcttcat caagaagaat atagttgccc
     5581 ttgagccttt ttgcctcaag ggtataatag ttatcagcat tattatgact aactgttgcg
     5641 cccagtttta tgagacctgt aaagtgggtg tcgagacgtc gccttcctat tttgtcacct
     5701 cccggtgtgg gaacgaatac tttaccgcat cgtgccagca gaggacccgc aatcattatt
     5761 gaaccacgta tacgctggcc cttttcaatc agacccggat cctttaaatt gtccggattc
     5821 agcgaggtgg cttcaaatgt tacggtggaa ccagatgact cttttgtgac cttgaccccc
     5881 agagaactga caaggtcaat cagattttcg acatcgagta tgcggggaat attttgaata
     5941 gtaaccttgt gggatgttag catggaggca catattacct gcagggcttc attttttgct
     6001 ccctgaggcg ttatttcacc tttaagtgac ctgcctccat gaacaataaa agaactcatc
     6061 aatgccggtt aaaccctttt ttcttctggg aatgtttatt ctgctgtttg ttgtgcttgt
     6121 ttgtctgctg ttttttctta ttctgcgcca taagttcttt tgcctcaggt attttcatgc
     6181 cttccggtat tgtaagcttc ccacctgata gatatttaag atcactgaga atgatgtcat
     6241 ccgatacctg tggacggttc catgtgatgt atagcttctt catctgattg atgatgagat
     6301 aggttaaata atctttctct tcaccgtcag gcatcttcga aacagcatca atcatcatct
     6361 caatgttctt gccgtagtgc agaaaccgga tgttaccctg tggatatggt atcctgttag
     6421 gcttttcagc cagtttcgac ttctcaggcg gaggataggg agagtcaatg tcgaggtcac
     6481 aatcagctat cagagccaga tgatcccata acttatgctt gaagtcctca acatccctta
     6541 gttgtggatt caaattaccc attatatgga taattgttgc agcagccctg ttcctctcat
     6601 cccggtcctt gattgtcttc aggtaatcaa ccattttctg gatattcctg ccgtattcgg
     6661 gaagaaccat ccttttgcgc tgagtattat aatcaaaacc catcagatat gttttacctg
     6721 gcaaaactaa taattaaatg tcaataaata aaagaattca agttgtgcaa taaaaaaaga
     6781 tttaaggatt tttgattaat cagttttata attttgagca ggcaccggtt aaaaaatgtt
     6841 atgagatgaa aaaatacctt cttctgtttt ttgcgtttct cttttcttat ttgctggctt
     6901 tcgg
//
LOCUS       DAOSNB010000126         6885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11078_length_6885_cov_4.210688, whole genome shotgun sequence.
ACCESSION   DAOSNB010000126 DAOSNB010000000
VERSION     DAOSNB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11078_length_6885_cov_4.210688"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /gene="aroE"
                     /locus_tag="PLG42_06265"
     CDS             <1..338
                     /gene="aroE"
                     /locus_tag="PLG42_06265"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOU30846.1"
                     /translation="VLGQLSIKVLFVSRIKKPECITYEDINEEVIDTTMLIVNTTPVG
                     MYPLTDNKPDIPYHLLSPSHILYDLIYNPEQTLFLKEGLRRGCTIINGLEMLRLQAEK
                     SWEIWNNPD"
     gene            complement(335..1090)
                     /gene="truA"
                     /locus_tag="PLG42_06270"
     CDS             complement(335..1090)
                     /gene="truA"
                     /locus_tag="PLG42_06270"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOU30847.1"
                     /translation="MTTRYFVFLSFKGTKYFGWQIQPNSITVQKVVDEALSTILREKI
                     STIGAGRTDSGVHAKFFCAHFDSIYNDLETRKNLLTRLNRFLPVDIAVQRIKKVRNDA
                     HARYSAVSRTYKYYITKNKDPFSEDTSWFVPYHLDLDCMNKACLILTKHSDFTSFSKL
                     HSNAKTNICNIHYAKWEDEDNLLIFTIKADRFLRNMVRAIVGTMVELGRKKINLDDFE
                     KIINARDRCKAGKSVPAKGLFLEDIEYPGDIFS"
     gene            1431..2768
                     /locus_tag="PLG42_06275"
     CDS             1431..2768
                     /locus_tag="PLG42_06275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU30848.1"
                     /translation="MTEQKSTQAAGMAFMGMIFFLFGFVTTFNITLADKFKAVFELTN
                     FETQLVNFAFFLTYFLLSFTAGGIIKKIGYKGGVILGLLLVALGSFLFFPAAKALSFP
                     FFLIAIFIMASGVVFLQVAANPYVTALGPSETASARLNLTQALNSIATYLAPIIASVF
                     VFKTTAEAALSAAEAAQSVPTPFIIIAVVVIIIAVAIYFMKLPVIPTQGIERKSVWKY
                     SHVLLGAVGIFCYVGAEVGSAAMLQRYFQEAINIAQSDAAKMIALYWGGAMIGRFYGS
                     FMLSKIEASKKYMYTAIVIAFAIFVGWFVRREITDGLIFAGIAVVNYLAFLIGKGKPN
                     RSLAVFGIIAALLLLVVMFASGNIILWLGLSVGFFNSIMFPNIFTLGVDGLDKGELSM
                     ASGLINSLIVGGAVVPVLMGIVADSAGVRYAFILPIICYLYIVFFALVGSKHK"
     gene            2905..4188
                     /locus_tag="PLG42_06280"
     CDS             2905..4188
                     /locus_tag="PLG42_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOU30849.1"
                     /translation="MKTSEYLIKSINGGDNKIFRELYGNEPAELKRHARRYETLLRQF
                     EENFGKKEVEIFSSPGRTEIGGNHTDHNWGRVLAGAVNLDNICVAAKNNKNIIRIVSE
                     GYPRFEVDLASLAPVAAEKYTSAALVRGICSRFKESGYKIGGFDACIDGGVPKGSGLS
                     SSASFEVLIGVILNELFNDGKIDPVQNAIIGQYSENNYFGKPCGLMDQVAIAMGGLIT
                     IDFKEPANPVVKKVNFDFTSTGYSLVITDTGGSHADLNDEYASIPADMKAVAAELGAK
                     VLRQVTLDQIIEIAPRIREKVGDRAILRAIHFQKDNQRVVDQVNALERNDFHAFLEMV
                     VESGYSSYMYNQNIFAVINPREQGVSLALALSELVLKGEGAWRVHGGGFAGTIQAFVP
                     KNLLEKYLNTLEHFFGKGACKTLFIRHQGACKVEL"
     gene            4208..5260
                     /locus_tag="PLG42_06285"
     CDS             4208..5260
                     /locus_tag="PLG42_06285"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOU30850.1"
                     /translation="MKIIPEESFKTVHNEKPVNLYTLKNKNGLIAQITNYGAIIVSIF
                     IPDKNGNFADIVQGYDTAKEYIMGNGPYMGAVCGRCANRIGGGKFILGDREYKLAVNN
                     GPNHLHGGLIGFNKVLWDVVSSNNSSVEMQYLSRDGEEGYPGNLNVKVKYSLTDENEL
                     RIDYNASTDKSTVVNLTSHSYFNLAGEGSGPVYDHELMINARYFTPTDETNLPTGEIL
                     SVKGTPMDFTSPRKIGEGIDSDYIHIKYGAGYDHNWVLSHRCGTLGLAAIARDPKSGR
                     IMEVYTTQPGLQLYTANWIDNERGKGGKIYGRRWAFCLETQHFPDAINKPHFPSVILN
                     PGETYRHTCIYKFKAE"
     gene            5363..6568
                     /gene="gluP"
                     /locus_tag="PLG42_06290"
     CDS             5363..6568
                     /gene="gluP"
                     /locus_tag="PLG42_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HOU30851.1"
                     /translation="MTNEKNKFLFPLIVMASLFFLFGFITTMNNSLINYLKDAFGLNE
                     VEKQLPNTFFYGAYIFSIPVGYLLNRIGYKNGVLSGLALVSLGFFLCIPGVTLGYYGF
                     LSAVSVFAIGVVLLQVAANPYVNALGKPETAASRLTLTNALNSLATVIAPIFVSMLIV
                     SSSSGASADPKAVQGPFAGIGIVTLVIVIIMFFLKLPEIKEGEETEGVVQEYKSSAYK
                     YPHMWLGSLAIFFYMGVEVGIPSFFADYSAKLGFSYDGEMRTKLLAYYWAGLMVGRIA
                     GIFILRKYSARQILSFNATFGALMLLLSLVLGSFGMNSAALVLFLGTGLMHSIMWPCI
                     YNLALEDLGPHTKVGSGVIATSVIGAALLPIMMGGIQKSVGLIAAICCLFVYYAYITY
                     FALKGSRIR"
     gene            6581..>6885
                     /locus_tag="PLG42_06295"
     CDS             6581..>6885
                     /locus_tag="PLG42_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOU30852.1"
                     /translation="MIKRGFASDNNSGVHPLILEEIHAVNQGHALGYGADVYTSQAEV
                     IFKQHFGEDTEVYFVFNGTAANVLGITGVLKPWNSVITAYTSHIEQDECGALENFAG"
BASE COUNT         2041 a         1221 c         1417 g         2206 t
ORIGIN      
        1 atgttcttgg acaattgagc ataaaagtac tttttgtttc acgtataaaa aagccggaat
       61 gcataaccta tgaggatatt aatgaggaag ttatcgacac tacaatgctg attgtcaata
      121 ccacaccggt tggaatgtac ccactgactg ataataaacc ggatatacct tatcatttgc
      181 tttccccgtc acatattctg tacgatctta tttataatcc cgaacaaact ctgtttctca
      241 aagaaggttt aagaagaggg tgtacaataa taaacggact ggaaatgctg aggctgcagg
      301 ctgaaaaatc gtgggagata tggaataatc ctgattagga aaaaatgtct cccgggtatt
      361 caatgtcttc aagaaacaaa ccttttgccg gaacagattt cccagctttg catctgtcgc
      421 gggcattgat aatcttttca aaatcatcca gatttatttt cttgcgccct aattcaacca
      481 ttgtccctac aattgctcta accatatttc tgagaaatct atcggcttta atggtgaaaa
      541 tgagaagatt atcttcatct tcccattttg cataatgaat gttgcaaata tttgtttttg
      601 catttgaatg cagcttactg aaacttgtaa aatctgaatg ttttgtcagt attaagcagg
      661 ctttgttcat gcagtctaaa tcaagatggt aaggcacaaa ccatgatgta tcttcactga
      721 aaggatcttt attcttggtt atatagtatt tgtatgttct ggaaactgcg ctataccttg
      781 cgtgtgcatc attcctaacc tttttaattc tttgtactgc aatgtcaaca gggagaaacc
      841 tgtttaatct tgtcaaaagg ttttttctgg tttccagatc attataaata ctgtcgaaat
      901 gtgcacagaa aaatttcgca tgaacacccg aatccgttct gcctgctcca atagttgaaa
      961 tcttttccct tagtatggta ctcaaagctt catctacaac tttctgaacg gtaattgagt
     1021 tgggctgaat ttgccatccg aaatattttg tgcctttgaa tgaaagaaat acgaagtatc
     1081 gtgtagtcat gtgttttatt tactgtaaat atattcaatg tataaatttc ttttttccgg
     1141 ttaattaaca ataattaact caaaatgctt ttcggaatat ccacaatttg cttatttgct
     1201 gaataacaga ttactattga aatttgtcct gattaatcaa ttttgtatta aataaattat
     1261 aatactaata atcaaacaat tatcataagg gtccatttac tgaaaacaaa tattttgttc
     1321 tctggctatg aagtaaataa tatttcaata tttttgaaaa ttcatcttaa aatgtaaatt
     1381 tacaatcgtt aaaactaacc agaacagtta aaccttaaaa ctatatcact atgacagaac
     1441 aaaaaagtac acaggcagcc ggtatggcat ttatggggat gatttttttc ctttttggtt
     1501 ttgtaaccac atttaatatt acactggctg ataagtttaa agctgttttt gaattaacga
     1561 atttcgagac tcagcttgtc aactttgcat tctttttgac ctattttctt ctctccttta
     1621 ctgcaggggg gattattaaa aaaatcgggt ataaaggagg tgttattctg ggtctgttat
     1681 tggtggcatt aggtagtttt ctgtttttcc ctgccgccaa agctctttct tttcctttct
     1741 tcctgattgc aattttcatt atggcaagcg gtgtggtatt tctgcaggta gcagccaatc
     1801 cttacgtcac ggctcttgga ccttcagaaa cagcatcagc aaggctgaat ctgacacagg
     1861 cgcttaattc aattgcaact tatcttgctc cgatcattgc gagtgttttt gtgttcaaga
     1921 caaccgctga agcagcactg agcgcagctg aagctgccca gtcggttcca acacctttta
     1981 ttataattgc agtagtggtt attattattg cagttgcaat ctattttatg aagcttcctg
     2041 taatacccac acagggtatc gaaagaaaaa gtgtgtggaa atattcccat gttttactcg
     2101 gagccgtggg tatcttctgc tacgtgggtg ctgaggttgg ctctgctgca atgcttcaga
     2161 ggtacttcca ggaagccatc aatattgctc agtccgacgc tgcaaaaatg attgctcttt
     2221 actggggcgg tgctatgatt ggaagattct atggttcatt tatgttatca aaaattgagg
     2281 catccaagaa gtacatgtat accgctattg taattgcttt tgcaatcttc gtcggatggt
     2341 tcgtaaggcg cgaaattact gatggattaa tatttgccgg aatagcagtt gtaaattacc
     2401 ttgcatttct gattggtaaa ggaaaaccca acaggtcact tgcagtgttt ggaattatag
     2461 ctgcattatt attgcttgta gtaatgtttg cttcaggcaa tattatcctc tggctgggtc
     2521 tttccgttgg gttttttaac tcaataatgt ttccaaatat ctttactctt ggtgttgacg
     2581 gccttgataa gggagaactt agcatggcat caggtcttat taacagcctg atagttggtg
     2641 gcgctgttgt tccggttttg atgggaattg tggctgacag tgcaggtgtt agatatgcat
     2701 ttattcttcc tattatctgc tacctgtata ttgtattctt tgctcttgtg ggaagcaagc
     2761 ataaataaca tctgtcttta ttttttggca tctgatttta atttgaaaga ggttgtctgg
     2821 agatgtttta gacaacctct ttaacataat tttgtatttc atattatata tttgtttttc
     2881 cggataacat aaaaacatta aaaaatgaaa acttctgaat atctcatcaa atcaattaat
     2941 ggcggtgata ataaaatttt ccgtgaactt tacggtaatg aacctgctga actgaaaaga
     3001 catgcccgga ggtatgaaac tcttttacga cagttcgaag aaaactttgg caaaaaagaa
     3061 gttgaaattt tcagttcacc gggcagaaca gaaataggag gaaaccatac cgaccataac
     3121 tggggcaggg tacttgctgg agcagttaat ctcgataata tatgtgtggc agctaaaaat
     3181 aataaaaaca taattcgtat tgtatcagaa ggttatcctc gttttgaagt agatctggct
     3241 tcccttgcac ctgttgctgc cgagaaatac acctctgcag cacttgtacg aggaatatgt
     3301 tcacggttta aggaatcagg atacaaaatc ggaggctttg atgcatgtat tgatggtggt
     3361 gtgcctaagg gttcaggact aagctcctct gcttcatttg aagttcttat aggtgtaatc
     3421 cttaatgaac ttttcaacga cggaaaaatt gaccccgttc aaaatgctat aatcggacaa
     3481 tattccgaaa ataattattt tggtaaacct tgcggtctta tggatcaggt tgcaattgct
     3541 atgggaggcc tgattaccat tgatttcaag gaacctgcaa atccagttgt gaaaaaagtc
     3601 aattttgatt ttacttcaac tggatattca cttgttataa ctgatacagg tggcagccat
     3661 gcagatttga atgatgaata tgcttcaatt ccggccgaca tgaaagcagt tgccgctgaa
     3721 ctgggtgcaa aagtgctcag acaggtaact ctcgaccaga taattgaaat tgctcccaga
     3781 ataagggaaa aagtaggtga tagagcaata cttcgtgcga tacacttcca gaaagataac
     3841 cagagagtag ttgaccaggt taatgcactt gaaagaaatg atttccatgc atttctggaa
     3901 atggttgtag aatcaggtta cagctcttac atgtataatc agaacatttt tgctgttata
     3961 aatcccagag aacagggggt ctctcttgct cttgcattga gtgaacttgt actgaaaggc
     4021 gagggtgcat ggcgtgttca tggaggcgga tttgccggaa ctattcaggc gtttgttccc
     4081 aaaaaccttc tcgaaaaata tttaaataca cttgaacact ttttcgggaa aggtgcatgc
     4141 aaaactctgt ttatcagaca ccagggcgct tgtaaagtag aactataatc aactaatgtg
     4201 aattagtatg aaaataattc cggaagaatc attcaaaaca gtccataatg aaaaacccgt
     4261 aaacctttat actttaaaaa ataaaaatgg actgattgcc cagattacca attacggtgc
     4321 tattattgtt agtatattca ttcctgataa aaacggaaat tttgccgaca ttgttcaggg
     4381 ttatgatact gcaaaagaat atatcatggg aaacggtcct tatatgggag cagtttgtgg
     4441 caggtgtgcc aacagaatag gaggaggaaa atttattctt ggtgacaggg aatacaaact
     4501 ggcagtaaat aatggaccga atcacctgca tggaggctta atcggattca ataaagtatt
     4561 atgggatgta gtatcctcaa ataattcatc ggtagaaatg caatatttat cccgcgatgg
     4621 ggaggagggt tatcctggta atctgaatgt taaagtaaaa tattctctta cagatgaaaa
     4681 tgaattaaga attgattata atgcttctac tgataagtct acggttgtca atcttaccag
     4741 tcattcctat ttcaaccttg ctggagaagg ttcaggtcca gtttatgacc atgagcttat
     4801 gataaatgca aggtatttca ctcctactga tgaaacaaat ctgcctaccg gagaaatact
     4861 gagtgttaaa ggaactccca tggattttac ttcaccaagg aaaataggag aggggattga
     4921 cagcgactat atccatataa aatatggtgc aggttatgat cataactggg tattgagtca
     4981 ccgctgcggt acacttggac tcgctgcaat agctcgtgat ccaaagtcgg gtcgtattat
     5041 ggaagtctat acaacacaac ccggactaca gctttatact gcaaactgga tagataatga
     5101 aagggggaaa ggtggaaaaa tatacggaag acgatgggca ttttgccttg aaacacagca
     5161 ttttccagat gcaatcaata agcctcattt cccttctgta atattgaatc cgggtgaaac
     5221 ctacaggcat acgtgtattt ataaatttaa agctgaataa ataatatgat tgtgctgggc
     5281 tatgtttttg aactataaat aaaatacaaa aaccgattct gaatgtaaac agttaacctt
     5341 taatttgtta aaaaataaaa ctatgacaaa cgaaaaaaac aaatttctgt ttccgttaat
     5401 tgtaatggcc tcactattct ttttgtttgg ctttattaca acgatgaata actcgctaat
     5461 aaactatctt aaagacgctt ttggtcttaa tgaagtagag aagcagcttc ccaacacttt
     5521 cttttatggg gcttacattt tttcaattcc tgttggttac cttctaaaca ggattggtta
     5581 caaaaacgga gtactttcag gcctcgctct ggtgagcctt ggttttttcc tgtgtatacc
     5641 gggtgtaaca ctgggttatt acgggttcct cagcgctgtt tcagtatttg caataggggt
     5701 ggtattgctg caggtagctg ctaatccata tgtaaatgcg cttggtaaac cagaaacagc
     5761 agccagtcgt ctgaccctta ctaatgcact taactcactg gctactgtaa ttgctcctat
     5821 attcgtttcg atgcttattg tatcgtcttc aagcggggcc tcagcagatc caaaggcagt
     5881 ccagggacca ttcgccggaa ttggaattgt aactctggtc attgtgataa ttatgttctt
     5941 tcttaaactg ccagaaataa aagaaggaga agaaactgaa ggagttgtac aggaatataa
     6001 atcgagtgct tacaaatatc cacacatgtg gcttggatca cttgcaattt tcttttatat
     6061 gggtgtggaa gtcggaattc ctagcttctt tgctgattat tctgcaaagc tgggattttc
     6121 gtatgatggt gagatgagaa caaaactgct ggcatactac tgggcaggtc ttatggtagg
     6181 ccgtatagca ggcatattca ttctcagaaa atattctgca agacaaatac ttagtttcaa
     6241 tgctactttt ggagctctaa tgttgcttct ttcccttgtt ctgggctcat ttggaatgaa
     6301 ttcagcagct cttgtcttat tccttggaac aggcttgatg cactcaatta tgtggccatg
     6361 tatttataat ctggcacttg aagatctcgg acctcatacc aaggtagggt caggagttat
     6421 tgccacctct gtgataggcg cagccttgct gccaattatg atgggaggca ttcagaagag
     6481 cgtcggacta attgcagcaa tatgctgtct gtttgtttat tatgcttaca ttacatattt
     6541 tgcattaaaa ggttcaagga tcagataagt gcaggataat ttgattaaga gggggtttgc
     6601 aagcgacaac aattcaggag tccaccccct tattcttgaa gagatacacg ctgttaacca
     6661 ggggcatgcg ctgggttatg gtgctgatgt ttacacatcg caggcagaag taatttttaa
     6721 gcaacatttt ggcgaagaca ccgaagtata ctttgtgttt aatggaactg cggcaaatgt
     6781 tcttggtatt acaggggtac taaagccatg gaattctgtc ataactgctt atacttctca
     6841 tattgagcag gatgaatgcg gggctcttga gaactttgca gggtg
//
LOCUS       DAOSNB010000127         6823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11209_length_6823_cov_4.369829, whole genome shotgun sequence.
ACCESSION   DAOSNB010000127 DAOSNB010000000
VERSION     DAOSNB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11209_length_6823_cov_4.369829"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLG42_06300"
     CDS             complement(<1..966)
                     /locus_tag="PLG42_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30853.1"
                     /translation="MKTFYKAGRLSMIYEKGALRYISSGRNELIRMIYPAVRDRNWET
                     VIPRIVEEKIDRSDDAFTVKIRCLYEYDRIKFDAEYIIEGHYDNTLILVMNGEALETF
                     EKNRIGFCVLHPIEPYAGQNCLIEHTDGTIEQSIFPEDISPHQVFRDIKSMKWVNKGI
                     TCQLQFEGDIFETEDQRNWTDNSYKTYSTPLSLPHPVTIEKGTRIYQKVVFSASGVTE
                     TFAAKDDIIHIELVPEEAYTFPSIGICRSSSGRELTLKEIKMLRAAGFSHYRTDIHLF
                     SPDWPVAADLSLNESIELGWPLEVALFFTGDIKQQARTFAEWLYSR"
     gene            1158..2804
                     /locus_tag="PLG42_06305"
     CDS             1158..2804
                     /locus_tag="PLG42_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30854.1"
                     /translation="MSSSSGNIYPLKGLIVVLPIIMIVTIVNFSCRIKSSQLQLVQDT
                     LLLSDTIQYHPVRINKSDGSILPWFSADKGESYDTVILLVWNFWKNMETDSNGLKYYM
                     NHQVWAPWHDMRGLGGDQINMALSSWALLYAYTGDSEIIENMKYMADNYLERSLSGPD
                     DKWPYIPYPYNTEVHSGKYDGDMINGKGFTQPDKAGTFGYELVNLYKITGEGKYLEAA
                     KRIALTLSRKMTEGDSLHSPLPFRVNAKTGEIAPLLERRITGRKVGEACYTTNWTGTM
                     QLFEELAKLDDANKEIYMKSFSVILEWMKKYPLKTNKWGPFFEDVPGWSDTQTNAISF
                     AMFILQHRALFPDWKEDVKVIIDWTYRELGNHEYEKYGVTVMNEQTAYRVPGNSHSSR
                     QASVELMYAALSGDTSRVRNAVRTLNWATYTVAPDGRNRYIRDDIWLTDGYGDYVRHY
                     IRAMGTMPQLAPSSKDRFLKTSSIVRSIQYSPEEIIYSTYDEAEDILRLTKKPRSVTC
                     DGTKLIESTDDIPESYSWKPLEKGGVLIVRHTGKKVEIIF"
     gene            2810..4345
                     /locus_tag="PLG42_06310"
     CDS             2810..4345
                     /locus_tag="PLG42_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOU30855.1"
                     /translation="MKNELYIASLGCILLLPACGQKNYEKPNIVFILADDLGYGDVSF
                     LNPDSKIPTPNIDRIARTGVTFTDAHSSSAVSTPTRYGLLTGRYSWRSEMKSGVLNGY
                     SEALIPESRKTIAQMLREVGYNTACIGKWHLGWKWNNIEKGNDSVDFSKPITDGPTER
                     GFDYFFGISASLDMPPYVYVENDMPTSMPDRITTGNNISYGNPGYDGSMWREGPTGSD
                     FFHIECTPEFFRRAGKYIEEKAREKKPYFLYLALPSPHTPILPTDEFKGKSGINPYAD
                     FVMMVDAEVGKLLDIIEGTGESENTIIVFTSDNGTAPWADIKTLESKGHMTSYIFRGH
                     KADLYEGGHRIPCFVSWPARIRKPHVVGQTICLNDFYATFAAVTGYKIADNEAEDSYN
                     LLPALLKPGYRKIIREATVHQSVNGNLAIRKGDWKLLMTPGSGGWSFPRPGREEEGLP
                     PVQLFNMKDDPSEQKDLQAEYPEIVKELTELLKKYIEDGRSTPGIPQKNEGEFPAKLR
                     FWE"
     gene            complement(4347..4598)
                     /locus_tag="PLG42_06315"
     CDS             complement(4347..4598)
                     /locus_tag="PLG42_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HOU30856.1"
                     /translation="MERRWDLTDGNKPLLAKEGVFYISYLEKGGTIKIKNMPEGLPFY
                     WFNPKTGETTTGGKTTSEGVFTSPDNNPWVLIAGNKVFN"
     gene            complement(4640..4882)
                     /locus_tag="PLG42_06320"
     CDS             complement(4640..4882)
                     /locus_tag="PLG42_06320"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOU30857.1"
                     /translation="MLKSSINYDNPYISVDVWAQFISDNNDTLIRPAFWDGGNIWQIR
                     FAPPDSGSLWRWTTFSEPFDKGLSGKTGYSDQQHIM"
     gene            5041..5787
                     /locus_tag="PLG42_06325"
     CDS             5041..5787
                     /locus_tag="PLG42_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30858.1"
                     /translation="MKKVKIVLIISVFVVYSFLSNASAQTGIDIETGLVFTGYNDVRI
                     PGNTGTLFSLSDDLSAKEKIFYRIRINHSFGNRHDISVLYAPLTIVSSGIFTDNVIFE
                     GVLFQSGRDVEADYKFNSYRLTYRYDIIKRDKLEFGIGFTAKIRDAKIALRSGGIESI
                     KTNVGFVPILNFRLLWNVKDNLGVLLYGDALAAPQGRAEDVLLALQYKVNEKVTLKGG
                     YRILEGGADNDEVYSFALFNYAVLGVTFKL"
     gene            5911..>6823
                     /locus_tag="PLG42_06330"
     CDS             5911..>6823
                     /locus_tag="PLG42_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU30859.1"
                     /translation="MKLGFVSAILPELPLEELVDYAAHLGYKCIEVCCWPVGKAERRY
                     AGVTHIDVDALDEKRASEIREYFNRNGVEISGLGYYPNPLDPDPEKRAFYASHIKKVI
                     DAAALLGVNRMNTFIGRDKTKNVEGNFEMFRQVWPGIIRHAEERGVKVGIENCPMIFT
                     YDEWPGGCNLATTPVIWRRMFSEIKSDFFGLNFDPSHMLWQQMDYIKPLYEFKDKIFH
                     IHLKDARLHRDRLDEVGIMATPLQFHTPKLPGLGDIDWAKFISTLYETGYDGPACVEV
                     EDRAFEGSLDDRKKSLALAKKYLDQFII"
BASE COUNT         2039 a         1303 c         1491 g         1990 t
ORIGIN      
        1 tcttgaataa agccactctg caaacgtacg agcttgttgt tttatatcgc ctgtaaaaaa
       61 caatgcaact tccagtggcc agccaagctc aattgattca ttcagcgaaa ggtcggctgc
      121 cacaggccag tcgggtgaaa aaagatgaat atcggttcgg taatggctga accctgcggc
      181 acgcagcatt tttatttcct tcagggtaag ctccctgccc gaagatgaac ggcatattcc
      241 tatggaaggg aatgtataag cctcttccgg taccagctca atgtgaataa tatcgtcttt
      301 cgcagcgaaa gtttctgtga caccggatgc actgaagact actttctggt aaattcttgt
      361 tcctttttca attgttaccg gatgaggcaa agaaagaggt gttgagtagg ttttgtagga
      421 attgtcagtc cagttccgct ggtcctctgt ttcaaatatg tctccctcga actgcaactg
      481 gcatgttatt cctttattta cccatttcat tgactttatg tctctgaaaa cctgatgcgg
      541 gctgatatcc tcgggaaaaa tcgactgctc aatggtgcca tcggtatgtt caataagaca
      601 attctgacct gcataaggtt ctatcgggtg aagaacacaa aatcctatcc ggttcttttc
      661 aaatgtctcg agtgcctcac cattcatcac caggataaga gtattatcat aatgtccttc
      721 aataatatat tcagcgtcaa attttattct gtcatattca taaagacatc tgattttcac
      781 tgtaaatgca tcatccgacc ggtcgatttt ttcttcaaca atacgtggaa tcacagtttc
      841 ccagttccgg tctctcacag caggatatat catcctgata agttcattcc gccctgagga
      901 aatgtatctc agagcaccct tctcatatat catcgacagc cttcctgctt tgtaaaacgt
      961 tttcattttt caagattatt gggctgaaat agatttgtta attgaatatt tccctgcgat
     1021 ataaaatctt tcagatgact gctttttaaa ccataatcct tctttttgcg gtttggaata
     1081 aatttgtttt gctgtatcaa aaaaattgta aatcttcaca taaaaataga aatttatttt
     1141 tacataaatc tcaagccatg agcagttcat caggaaacat ttacccattg aaaggattaa
     1201 tagttgttct tccgataatt atgattgtta cgattgtaaa tttttcatgc aggataaaat
     1261 ccagtcaatt gcagctggtt caggatactt tattgctcag cgacacaatt caatatcacc
     1321 cggtaaggat aaataagtct gatggctcca ttctaccatg gttttcagcc gacaaaggtg
     1381 aatcgtacga tacagttatt ttactggtat ggaatttctg gaagaatatg gagactgatt
     1441 cgaacgggct aaaatattac atgaaccacc aggtgtgggc accctggcat gatatgcgcg
     1501 gactgggagg tgaccagatc aatatggcgc tttcatcatg ggcacttcta tatgcttata
     1561 ctggtgactc cgaaatcatt gaaaatatga aatatatggc tgacaattat ctggagaggt
     1621 ctctttccgg acctgatgat aaatggccat atattcctta tccgtacaat actgaagttc
     1681 attcggggaa atatgatggt gatatgataa atggaaaggg atttacccag cccgataaag
     1741 caggtacttt cggttatgaa cttgtgaacc tttacaaaat aaccggcgag ggcaaatatc
     1801 ttgaagcggc aaaaagaatt gccctgacgc tctcccggaa aatgacggaa ggagacagtc
     1861 tgcattcgcc tcttcctttc agggtgaatg caaaaacagg agaaattgcg cctcttctcg
     1921 agaggagaat aactggcagg aaggtgggag aggcttgtta tacaaccaac tggacaggca
     1981 ccatgcaatt gtttgaggaa cttgcaaaac tggatgatgc aaacaaagag atctacatga
     2041 aaagtttttc ggtaatcctt gagtggatga agaaatatcc actgaaaaca aataaatggg
     2101 gtccgttttt tgaagatgtc ccgggatgga gtgacacaca gaccaatgcc atcagctttg
     2161 caatgtttat acttcagcac agggctctct ttcctgattg gaaggaagat gtgaaagtca
     2221 taattgactg gacatacagg gaacttggca accatgaata tgaaaaatac ggtgttacgg
     2281 taatgaacga gcagacagcc tacagggttc cgggaaatag ccattcgtcg cggcaggcct
     2341 cggtagaact catgtatgct gctctctcag gcgatacctc tcgtgtgaga aatgcagtca
     2401 ggactcttaa ctgggctact tatacagttg cacctgacgg ccgtaaccgt tatatacgtg
     2461 atgatatatg gcttactgat ggttatgggg attatgtcag acattatatc agagcaatgg
     2521 gcaccatgcc tcaacttgct ccttcgtcaa aagacaggtt tttaaaaact tcatcaatag
     2581 taagatcaat tcaatattca cctgaagaaa taatttacag tacttatgat gaagcggaag
     2641 acattttaag gcttactaaa aagccccgtt cagtaacatg tgatgggacg aagctgatag
     2701 agagcactga tgatattcct gaaagctatt catggaagcc tcttgaaaaa ggcggggtac
     2761 tgatagtaag gcatactggt aaaaaggttg aaataatatt ttaattaata tgaaaaatga
     2821 gttgtatatt gcaagtttag gatgcatttt gcttctgcct gcctgcgggc aaaagaatta
     2881 tgaaaagccg aatattgtat ttatcctggc cgatgacctt ggttatggtg atgtaagttt
     2941 ccttaatcct gattcaaaaa tacccacgcc caacattgac aggatagccc ggacgggagt
     3001 tactttcacc gatgcacatt catcttcagc tgtaagtacc cctacaagat atggattact
     3061 tactggaaga tacagctgga gaagtgaaat gaaaagcgga gtgctgaatg gttattcgga
     3121 agcattaatt cctgaaagca gaaaaacaat agctcagatg ctcagagaag ttggttacaa
     3181 tactgcctgt atcgggaaat ggcatctggg atggaaatgg aacaacatcg aaaaaggcaa
     3241 tgacagcgtt gatttttcga aacctattac cgatggtccc accgaaagag ggtttgatta
     3301 tttctttgga atcagtgcct cgctcgatat gccaccatat gtttatgtgg aaaatgatat
     3361 gcctacctca atgcctgaca gaataaccac aggcaacaat atttcatatg gtaatcctgg
     3421 ttatgatggt tcgatgtgga gggaaggacc aactggaagt gatttttttc atattgaatg
     3481 caccccggaa tttttccgca gggctggcaa atacattgaa gagaaagcac gcgagaaaaa
     3541 gccttacttc ctttaccttg cactgccatc gcctcatacc cctatccttc caacggatga
     3601 atttaaaggt aaatcaggca taaacccata tgccgatttt gtaatgatgg ttgatgccga
     3661 agtgggcaaa ctacttgata ttatagaggg aacgggtgaa agtgaaaata caattattgt
     3721 tttcacctct gacaatggta ctgcgccatg ggcggatatc aaaacactcg aatcgaaagg
     3781 acacatgaca agttatattt tcagaggcca caaagccgac ctttatgaag ggggtcacag
     3841 gataccatgt tttgtgagtt ggcccgccag gataagaaaa cctcatgttg ttgggcagac
     3901 aatatgtctt aatgattttt atgcgacttt tgcagctgtt acaggatata aaatagctga
     3961 taatgaggct gaagacagtt acaatcttct gccggcactc cttaaacccg gatacaggaa
     4021 gattataaga gaagcaacag tccatcagtc ggttaatggc aatcttgcaa taagaaaagg
     4081 cgactggaag cttttaatga cgccaggatc gggaggctgg agtttcccgc ggccgggaag
     4141 ggaagaggaa ggactgcctc cagtacagct ttttaatatg aaggatgacc catcagaaca
     4201 gaaagacctt caggctgagt accccgaaat tgttaaagaa ttaacagaac tattaaagaa
     4261 atacatagaa gatgggcgca gtaccccagg cattcctcag aaaaacgaag gggaatttcc
     4321 tgctaagcta agattctggg aataatttaa ttaaaaactt tgtttccggc aatcaacacc
     4381 catgggttat tgtcaggaga tgtaaaaacg ccttcagaag ttgtttttcc tccggttgtg
     4441 gtctcaccgg ttttagggtt gaaccagtaa aacggtaaac cctcaggcat atttttaatt
     4501 tttattgttc ctcctttttc gaggtatgag atataaaata ctccttcttt tgcaagtaat
     4561 ggtttattgc catctgtcag gtcccagcgc ctttccattt cggcaaagtc aaagccatta
     4621 aatgcttttg cagcatatcc tacataatat gctgttgatc ggaataccct gttttcccgc
     4681 tgagaccttt atcaaacggt tctgaaaagg ttgtccatcg ccataaagat cctgagtcgg
     4741 gcggagcaaa ccgtatttgc cagatatttc caccatccca gaatgccggt ctgataagtg
     4801 tatcattgtt gtcgctaata aactgagccc agacatcaac acttatatat ggattatcat
     4861 aattaatgct tgatttaagc actatttcgt tagtggtcta ttgttcaacc agttgtattt
     4921 cttttctgca cgaacaaaaa aatgccggta taataaccag aagaggcatt tttaattttt
     4981 tcattcaaat aagaactttc tgtttaataa tttgttttag taatataaaa ttaaaattaa
     5041 atgaaaaaag taaaaattgt tctgattatt tcagtttttg ttgtttattc ttttctctca
     5101 aacgcatctg cccagacagg tattgatatt gagacaggtt tagtttttac cggatacaat
     5161 gatgtgcgta ttccgggaaa tacgggtaca ttgttttccc tcagcgatga tctcagtgca
     5221 aaagaaaaga tattttacag gataaggata aatcattctt ttggtaacag acatgacata
     5281 tctgttctat atgcacctct gactattgtt tcttccggta tttttactga taatgtgatt
     5341 ttcgaaggag ttcttttcca gtcgggcagg gatgtagagg cagattataa attcaactct
     5401 tatcgtctta catacagata cgacattatt aagagggata agctggagtt tggtatcggt
     5461 tttacagcaa aaatacgcga tgctaaaatt gctttgcgtt caggtggaat tgaaagtatc
     5521 aaaaccaatg tcggatttgt acctattctg aacttcagac tgctctggaa tgtcaaggat
     5581 aatcttggtg tgttgcttta tggtgatgcg cttgctgcac ctcagggacg tgccgaggat
     5641 gtgcttctgg cattgcagta taaagtaaac gaaaaggtaa ctcttaaagg cggatacaga
     5701 atacttgagg gaggtgcaga caatgacgaa gtttatagtt ttgcactgtt taattatgct
     5761 gtacttggag tgacttttaa actctgaaac aaggttttat tctatcttaa ttaagacaca
     5821 ggtaaatctt ttcctgtctt tagcagatag gattttctcc ttttttcaaa aaagtttaat
     5881 tttaaatgat ttttaaaaaa ggaggaaatc atgaaactcg gatttgtaag tgctatttta
     5941 cctgagcttc cgcttgaaga gcttgttgac tatgccgctc atctgggata taaatgcatt
     6001 gaagtatgtt gctggcctgt aggaaaggcc gaaaggcgtt atgcaggcgt tactcacatt
     6061 gatgttgatg cgcttgatga gaagcgtgct tcagagataa gggaatattt caacagaaat
     6121 ggagttgaaa tttcaggctt gggatactat ccgaatccgc ttgatcctga ccctgaaaag
     6181 agagcttttt atgcgtccca tatcaaaaag gttattgacg ctgctgcact tcttggcgta
     6241 aacaggatga acacatttat tggcagggat aagacaaaga atgttgaggg gaattttgag
     6301 atgttccggc aggtctggcc cggtatcatc agacatgccg aagaaagggg cgtgaaggtg
     6361 ggcatcgaaa actgtccgat gattttcact tacgacgaat ggcccggagg gtgcaacctg
     6421 gcaaccacac ctgttatctg gcggaggatg ttctccgaga taaagagtga cttcttcgga
     6481 ttgaactttg atccctccca catgctttgg cagcagatgg attacattaa acctttatat
     6541 gaattcaaag acaaaatatt ccatattcat ttgaaagatg cccggctgca ccgcgacagg
     6601 ctcgatgagg tgggaataat ggcaacacct ctgcagttcc atactccaaa acttccaggc
     6661 ctcggcgata tcgactgggc aaaatttatt tcaactctat atgagacagg ttatgacggt
     6721 ccagcttgtg ttgaggttga ggacagagcc tttgaaggtt cactcgacga ccggaagaaa
     6781 tcactggctc tggcgaaaaa atatctggat cagtttataa tat
//
LOCUS       DAOSNB010000128         6801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11250_length_6801_cov_4.084346, whole genome shotgun sequence.
ACCESSION   DAOSNB010000128 DAOSNB010000000
VERSION     DAOSNB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11250_length_6801_cov_4.084346"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1779
                     /locus_tag="PLG42_06335"
     CDS             100..1779
                     /locus_tag="PLG42_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOU30860.1"
                     /translation="MVFSKYEKHLAFLFIVTFFFRFFSVTGQVAYIPPEKPKLIVSII
                     VEQLRYDQIEKLKNRFGEDGIRRLINEGTTFKNANFNYVLTQSSTGHATIASGTEPSF
                     HGIISDYWYVPLKNEIIYCTQDKEVGPVGGSYEQGLHSPAQLLTSTFSDELALATKGK
                     ARIFSVGLKESSAILSAGHAANGVFWFDNTTGTWMSSSWYMKELPAWVNDFNAMKLSE
                     TYLNNIWSPLREKEVYSDFLPDSNSFEAGFGGICWFPYDLKKMRSKGIPDGMKDYYLI
                     RETPFGNSFTKDFAIRLIKEEALGQDDITDFLSVCFSANDYIGHRFGPSSVEAADAIL
                     RLDADIAELIKFLNETIGKKNVLVYLTASHGVSEIPAVLESNRIPSGYFRPNQAMQLL
                     RLYLNAVYGQGEWVKGYWQNQVFLNRTLIEDARISLDEMQKKVARFLVQHSGVAAAFP
                     LSVFEANEFISGSLPDKASNNYIPQRSGDVLIVLSPGWVEKSDNRVTDHNSHYDYDTH
                     VPLIWYGWTVNRATVTRKVNMTDIAPTLSVMCGIPLPNACTGEPINELVIR"
     gene            complement(1782..2510)
                     /locus_tag="PLG42_06340"
     CDS             complement(1782..2510)
                     /locus_tag="PLG42_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOU30861.1"
                     /translation="MKSGILPSRKSVALLLDPDKAKGKALDNIIETAVSAGVDYILTG
                     GSLTFCNIDPLIKHVRNKCSIPVILFPGNLLQLNTSVDGIYFISLISGRNPELLIGNH
                     VIAAPFLKDIRDRVVPVGYILINCGSKTSVEYLSQTEAIPSDKPDIITATALAGEMLG
                     MKMIYLEGGSGAIKPIPSEVIREVKENITLPLAVGGGLKTAEQVKEAFNAGADLIILG
                     NGCEKRPALLAEACKIRDSFRKRK"
     gene            complement(2507..3148)
                     /locus_tag="PLG42_06345"
     CDS             complement(2507..3148)
                     /locus_tag="PLG42_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOU30862.1"
                     /translation="MGCITKHYLNEYSVLGVWKIEEDIDTLLGMVELDSDDRKRFREF
                     RSNSRKLEFLSVRALLAELLGKEAKIVYNKNNKPFLKDGSHFISISHSYRLTAILLST
                     NEQVGIDLEYMNANIAAFASKFMNKKEKVVKDPARVKYHLYLHWCAKEALYKICDKEG
                     ISIKNNIIIQPFVLKDSGEIKGVVKTDKINLTFDLSYTKYDNYAIVWTKKKIL"
     gene            complement(3159..3740)
                     /gene="gldD"
                     /locus_tag="PLG42_06350"
     CDS             complement(3159..3740)
                     /gene="gldD"
                     /locus_tag="PLG42_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOU30863.1"
                     /translation="MKRRKIEIIILAALLSLFISCREKTIPRPRGYFRIDFPEKQYIL
                     YDTSCPFIFEYPAYGKISYDLSDKAEPCWFNLYFPSFRATLHISYKRVQGNLPLIVKE
                     ANDFVYSHTIKADAISEQPWYNREERVYGILYDIKGNAASAIQFVLTDSLNHFLRGAL
                     YFSSIPNEDSLAPVIDFFRKDIVHFIESFRWKN"
     gene            complement(3721..5064)
                     /gene="gldE"
                     /locus_tag="PLG42_06355"
     CDS             complement(3721..5064)
                     /gene="gldE"
                     /locus_tag="PLG42_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOU30864.1"
                     /translation="MGSELSFPHVLSLGAVGLSAPGIKVISGFIILIILLMLSACMSG
                     SEVALFSLGPQDIKKMKSAKSRRSSIILNFLNKPEELLSTILVANNLINVGIVILSAF
                     LSGMLFDFSEHPLAGFLIEVVAITFMILLFGEILPKVYATRNHYSFSLFMAYPLLVLW
                     KILRPATLILTSTTRFLKEKKFRKRHSISMEDISDALELTSGKLDEEEKILKGIVKFG
                     NINVSAIMCPRVDVTALDIKTGFSEVIRIINESGFSRIPVYSGTFDNVTGILYAKDVL
                     PYSGYPDSFKWQSLLRPPYFVPETKKIDELLKEFQLKKIHMAIVIDEYGGTSGIVTLE
                     DILEEIVGEITDESDETVPLYKKVDDHTYIFEGKILLNDFYKVFEIEDDPFSEIRGDS
                     ETLAGLILEMTGEIPPKNKEINYGKFTFIVEASDKRRIKEIRVILKKDYFEKKKD"
     gene            complement(5086..5613)
                     /locus_tag="PLG42_06360"
     CDS             complement(5086..5613)
                     /locus_tag="PLG42_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOU30865.1"
                     /translation="MSLNRVILVGNVGKDPVIRYFDRGVAKATFPLATSETYTNQQGE
                     TITSTEWHNIVLWRALAEVAEKKVKKGTQLYIVGKIKTRSYVDKDGNNKYITEIIADT
                     MMILDRKQGTATTSSASPANHEADETGFNEPPVQAYNNSSNNKPIDIISEPENLTDPF
                     SDEQLMSKSDDDLPF"
     gene            5954..6241
                     /locus_tag="PLG42_06365"
     CDS             5954..6241
                     /locus_tag="PLG42_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOU30866.1"
                     /translation="MTKADIVNEIAKNTGIEKVTVQKAIEAFMETVKNSMVNGNNVYL
                     RGFGSFVIKKRAKKTARNISKNTTIIIPAHNIPAFKPAKSFVTKVKAHVKK"
     gene            6449..>6801
                     /locus_tag="PLG42_06370"
     CDS             6449..>6801
                     /locus_tag="PLG42_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU30867.1"
                     /translation="MESRDLIIDVGDEEISLALLEDKQLVELHKEKRNVKFNVGDIYL
                     GKVKKIMPGLNAAFVDVGYEKDAFLHYFDLGPQFRTLHKYYNQAVQRKGKIVPVHKFT
                     PEPDIDKDGKINQVLS"
BASE COUNT         1967 a         1239 c         1410 g         2185 t
ORIGIN      
        1 tcgaatcgta gcctgctaat tgcatctgat gttaaaaaga ctatttttgc tgattcggtt
       61 tttctgtaat taaatgagtt taacctaaca ggggaaagca tggttttttc aaagtatgag
      121 aagcatttag catttctttt tattgtaaca ttctttttcc ggtttttttc agtgacaggc
      181 caggttgcgt atataccgcc tgaaaagcca aagctgattg tttcgataat tgttgagcag
      241 ctcagatacg atcagataga aaaactgaag aacaggtttg gggaagacgg aatcagaagg
      301 ttgattaatg aaggcacaac atttaagaat gcaaacttta attatgtact gacacaatca
      361 tctacaggtc atgccaccat agcttcgggt actgaaccct cttttcatgg aattatatcc
      421 gattactggt atgtgccgct caaaaatgaa attatttatt gcacacagga taaagaagtt
      481 ggacccgtcg gtggcagcta tgagcagggt ttacattctc ctgcgcagct tctaacttcg
      541 acattttctg atgagcttgc tttagccaca aaaggaaagg caaggatttt ttcggttggg
      601 cttaaggaga gttctgctat tctctcggca gggcatgctg caaatggtgt tttctggttc
      661 gacaatacaa ccgggacgtg gatgtcgagt tcatggtata tgaaggagct tcctgcatgg
      721 gttaatgatt tcaatgctat gaagctttcc gagacgtatt taaataatat atggtcgcca
      781 ctgagggaaa aagaggtcta ttccgacttt ttacccgact caaacagttt tgaggctggt
      841 tttggaggca tatgctggtt cccgtatgat cttaaaaaga tgcgctcgaa aggcataccc
      901 gacggcatga aggattatta ccttatcagg gaaacccctt ttggcaacag ctttaccaaa
      961 gattttgcaa tcaggcttat taaagaagaa gcactcggac aggatgatat aactgatttt
     1021 ttatcagtat gtttctctgc aaatgattat atagggcacc gtttcggacc ttcatcggtt
     1081 gaagccgctg atgccatact caggctggat gcagacattg cagaattgat aaaatttctg
     1141 aatgaaacca taggtaagaa aaatgtactg gtatatctta cagcatcaca tggtgtatct
     1201 gaaatacctg ctgtgcttga aagtaacaga atcccttcgg gctatttcag gccgaatcag
     1261 gcaatgcagt tgctgaggtt atatcttaat gctgtttatg gccagggaga atgggtaaaa
     1321 ggatactggc agaatcaggt gtttctgaac cgtacactta ttgaagatgc cagaatttcc
     1381 cttgatgaaa tgcagaaaaa ggtagcaagg tttcttgttc agcattcggg tgttgctgct
     1441 gcatttcccc tatcggtttt cgaagcaaat gaatttattt caggtagtct tcctgataaa
     1501 gcttctaaca actatattcc acagcgttca ggtgatgttt taatagtgtt gagtccggga
     1561 tgggtggaga aatcagataa cagggttaca gatcataatt ctcattatga ttacgacacc
     1621 catgtgccac ttatatggta tggctggacg gtaaacaggg caactgttac aaggaaagta
     1681 aacatgacgg atattgcacc tactctttcg gtaatgtgtg gcataccatt gcccaacgcc
     1741 tgtactggag agccaattaa tgagctggtt atcaggtagt attattttct ttttctgaag
     1801 gaatctctta ttttgcatgc ttctgccagc agtgcaggtc gtttttcgca tccgtttcca
     1861 agtataatga ggtcggcacc tgcattaaag gcttctttaa cctgttcagc tgtcttcagt
     1921 ccgccgccaa cagctaaagg caatgtaatg ttctctttga cttccctgat gacttctgaa
     1981 gggatgggtt ttattgctcc gctgcctccc tcaagataga tcattttcat tccgagcatt
     2041 tctcctgcaa gagctgttgc ggttattatg tcaggcttat ctgagggtat tgcttctgtc
     2101 tgacttaaat actcaactga agtcttcgaa ccgcagttaa tcaggatata tcctaccgga
     2161 acaactctgt cgcgaatatc tttaagaaaa ggtgctgcaa taacatggtt ccctattaga
     2221 agctcgggat tgcgcccgga aatgagtgat ataaaatata ttccgtcaac cgatgtatta
     2281 agctgtagaa gattgccagg gaagagaata accggtattg agcatttatt cctgacgtgt
     2341 tttattaacg gatcaatgtt gcagaaggtt aaacttccac ctgtaagtat gtaatctact
     2401 cctgctgaaa cggctgtttc aattatattg tcgagcgctt ttcccttcgc cttgtcgggg
     2461 tcgagcagaa gagcgaccga cttacgggat gggagtatgc ctgatttcat aatattttct
     2521 tttttgtcca cacaattgcg tagttgtcat attttgtgta tgaaaggtcg aatgtaagat
     2581 ttattttgtc agttttaact actcccttta tttctcctga gtctttcaat acaaaaggct
     2641 gaataataat attattcttt atgcttatac cttctttgtc acaaatttta taaagggctt
     2701 ctttggcgca ccagtgaaga taaaggtgat attttactct tgcagggtct tttactactt
     2761 tttccttttt gttcatgaat tttgaagcga acgcagcaat atttgcgttc atatattcaa
     2821 gatctattcc cacctgttcg ttggtgctca acagtattgc ggtaaggcgg tatgaatggg
     2881 agatacttat aaaatgactt ccatctttca gaaatggctt gttgttttta ttgtatacta
     2941 tttttgcttc tttgccaaga agttcggcaa ggagtgctct cacgctcaga aattcaagct
     3001 tccgggaatt gcttctgaat tcacgaaatc ttttcctgtc atcgctgtcg agctcaacca
     3061 tccccagcag tgtatctata tcttcttcaa ttttccagac gccaaggaca gaatactcgt
     3121 tcagataatg ttttgttatg caccccatgt ttgctttgct aatttttcca gcggaaagat
     3181 tcaatgaaat gaacaatatc ttttcggaaa aaatcaatta ccggtgccag tgaatcttcg
     3241 tttggaatgg atgagaaata gagtgcgccc ctgagaaaat gattcagact gtcggtcagg
     3301 acgaactgaa tcgctgaagc agcattaccc tttatatcgt acaatattcc gtatactctc
     3361 tcttcccggt tgtaccatgg ttgttcactt atggcatcgg cctttattgt atggctgtaa
     3421 acgaaatcgt ttgcctcttt gacgataagc ggcagattgc cttgcactct tttgtagctt
     3481 atgtgcaaag tggctctgaa ggatggaaaa taaagattaa accagcatgg ttcagcttta
     3541 tcgcttaagt cgtaagatat ttttccatat gcaggatatt caaaaataaa aggacaggaa
     3601 gtgtcataaa gaatatattg cttttccgga aaatctattc tgaaataccc cctgggtctg
     3661 ggtatggttt tttccctgca tgatataaac agggataata atgctgccag gatgatgatt
     3721 tcaatctttc ttcttttcaa aatagtcttt tttaagtatt acccttatct cttttattct
     3781 tcttttatca gaagcttcta caataaaagt aaactttccg taattaattt ctttgttttt
     3841 cggaggaatt tcacccgtca tttcaagaat aagtcctgct agagtttctg aatcacccct
     3901 tatttcactg aacgggtcat cttctatttc aaaaactttg taaaagtcgt taagcagaat
     3961 cttgccttca aagatatatg tgtgatcatc aactttctta tacaggggaa ctgtttcatc
     4021 actttcatcg gttatttctc ccactatttc ttcaagtata tcttccagag tgacaatacc
     4081 ggatgtacct ccgtattcgt cgattacaat tgccatatgt atttttttca actgaaattc
     4141 tttcagcagt tcatcgatct tttttgtttc aggtacaaaa taaggaggtc tgagcaatga
     4201 ttgccatttg aatgaatcgg gatatcctga atacggcaga acatcttttg cataaaggat
     4261 acctgtcaca ttgtcgaatg ttcctgagta tacaggtatg cgtgaaaacc ccgattcatt
     4321 tataatacgg atcacttcac tgaagcctgt tttgatatcg agtgctgtaa cgtcaacacg
     4381 tgggcacatt attgcgctga catttatgtt gccgaatttt acaatacctt ttaatatttt
     4441 ctcttcttcg tcgagttttc ctgaagtaag ttcaagtgca tccgaaatgt cttccatgct
     4501 tattgagtgc cgtttccgga attttttttc tttcaggaaa cgggttgtgg atgtgagtat
     4561 taaggttgca ggtctcagaa ttttccatag cactaaaaga ggatatgcca taaagaggga
     4621 gaaagaataa tgatttcttg ttgcataaac tttgggcagt atttctccga aaaggagaat
     4681 cataaatgta attgcaacaa cttcaatcag gaaacccgca agtggatgtt cgctgaagtc
     4741 gaataacata ccggaaagga aggctgaaag gataacgatt ccaacgttaa taaggttatt
     4801 ggcaacaagg atagtgctga gcagctcttc aggtttgttt agaaaattga gtattattga
     4861 tgatctcctg ctttttgctg atttcatttt cttaatatcc tgaggcccga gagaaaagag
     4921 ggcaacttcc gaccccgaca tacaggcaga gagcataagc aaaatgataa ggattataaa
     4981 tccagaaata actttaatgc cgggtgctga taatccgacc gcccctaaac tcagaacatg
     5041 cggaaatgat aactcagagc ccaatggttt ctgtttttgt taatactaaa acggaagatc
     5101 atcatctgat tttgacataa gctgttcatc actgaaagga tcagtaaggt tttcaggctc
     5161 actgattatg tcaatgggtt tgttgtttga actattatta tatgcctgta cgggaggttc
     5221 attgaaacct gtttcatcag cctcgtgatt ggctggtgaa gccgatgaag tagttgccgt
     5281 gccctgcttg cggtcaagta tcatcatagt gtcggcaatt atttccgtta tgtatttgtt
     5341 gtttccgtcc ttgtcaacat acgaccttgt ttttatctta ccgactatat acagctgagt
     5401 gccttttttt actttttttt ctgcaacttc ggctagagct ctccacagca caatgttgtg
     5461 ccattcggta gaagtgattg tttcaccctg ctgatttgta tatgtttcac ttgttgcaag
     5521 tggaaatgta gcttttgcaa ctcccctgtc aaaatatctg attaccggat cttttccgac
     5581 atttccaacc agaataaccc tgttaagtga catagattta aatatgttat gatttatgag
     5641 gtaaagttaa taaaaaaaga actcggaagg aaaactattg gaacccgaaa cagattatat
     5701 ccatgtaatt agtaaatatg tgattataaa taaaatacgg ttgttttgat tattttgcca
     5761 gggatcacga ttgaatataa cgaagtatta tttcaggtgt tttttctttt taatattttg
     5821 ttctaaatta gtaccttgca aacagaaaga aataaaattt gatgtaagta tttcaattta
     5881 aataaatttt tatatttgca gggttgaaaa taactaatta acatagattg ttgaatttaa
     5941 aacttaagga aacatgacaa aagcagacat tgttaacgaa attgcaaaga acaccggtat
     6001 agagaaggta acggttcaga aagctattga agccttcatg gaaaccgtta agaactcgat
     6061 ggttaatggt aacaacgtgt atctccgtgg attcggaagc tttgtgatta agaagagagc
     6121 taaaaaaaca gccaggaaca tttcaaagaa tactaccatc attattcctg cacacaacat
     6181 tcctgcattt aaaccggcta agtcgtttgt tacaaaagta aaagctcacg ttaagaagta
     6241 gtttttatgc caagcggtaa gaagaggaag agacacaaga tggccacgca caagcgtaaa
     6301 aaacgcttga ggaaaaacag acataagaag aaataagaaa tcgaattgtg gtctgaatta
     6361 tctggacttt aaacctaaag atccccgggt ctttaggttt gttctatttt tttaatcttg
     6421 aaataattac agaatagctt ttataaaagt ggaaagcagg gatttgatta ttgatgtagg
     6481 ggatgaggag atttctctgg ctctactgga agataagcag cttgtagagt tacataagga
     6541 aaagcgcaat gtaaagttca atgtcgggga tatatacctt ggcaaggtta agaagattat
     6601 gcccggcctg aatgctgcat ttgtggatgt tggatatgaa aaggatgctt ttcttcacta
     6661 tttcgatctt ggtcctcagt tcaggacact tcataaatat tacaaccagg ctgttcagag
     6721 gaaggggaaa atagtcccgg tacataagtt tacgcctgag cctgatatag ataaagatgg
     6781 aaagataaat caggttctgt c
//
LOCUS       DAOSNB010000129         6781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11297_length_6781_cov_4.567053, whole genome shotgun sequence.
ACCESSION   DAOSNB010000129 DAOSNB010000000
VERSION     DAOSNB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11297_length_6781_cov_4.567053"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PLG42_06375"
     CDS             complement(<1..1902)
                     /locus_tag="PLG42_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOU30868.1"
                     /translation="MKILVSFLVMLPLAAFGQVYNDFEISGLQGWIQSPAGQWGIDSV
                     TPLSGRFSLWHCFDNSEAGSAQAAVRLMNLKPDRGTIKWSFQVRHGCDPSSSNNWSVF
                     LMSDAPASEMKPGGKVNGYAAGVNFTGYDDTLRVWKIKNGSAVTVVKTDINWQAIIGT
                     TQPATIFIERTPDGMWSIKIKLPGGEEKGVYKGIDNEIFEVEWFGIFYRYTSTRDRLL
                     WFDNLIIDGPFFQDTEPPQPVSCTIKSRRTIELIFNEKILPGFLNRSNFLLNSTFYPV
                     DDAQSTGTLSYLLNFDSDFINRQPSELLIKNLCDRDSNCISDLAISFIPLWAEHGDVV
                     ISEIMADPTPAVSLPVKEYLEITSRSEQPISLEGWALCTEGQKSYFPDVKINNGDYII
                     LCSVADTGLFKQYGAVVGLKYFPALTDSGKCLWIADSSGVMIHGIEYNSSWYGDRLKA
                     EGGWSLEMIDISCPFNYEGNWKASVSPSGGTPGRVNSVSGSYTDRYFEGITNVFPADS
                     FMIKVSFSEPVINFERLREAVRINGKVVNSIQALDPLEREFIIVSAEPFVRRRQYTLT
                     LLDDVTDYAGNRASKNSFIFGLPEPVTTRDVVFNELLFNPLPGDADFIEFYNVSDKII
                     DISSLSVASG"
     gene            1993..2985
                     /locus_tag="PLG42_06380"
     CDS             1993..2985
                     /locus_tag="PLG42_06380"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOU30869.1"
                     /translation="MVKTAVIGATGMVGRVMVKLLEERNFPVSELILAASDKSVGKEI
                     IFKGKPVKVISVKQAVESKPVFAIFSAGADVSKEWAPVFANNGTVVVDNSSAWRMDDN
                     IPLVVPEINSHVIKKGDRIIANPNCSTIQMVMALAPLHKEYKIKRIVVATYQSVTGTG
                     VRAVTQLENERKGIKGEMAYPHPIDLNCFPFGGKFLPSGYTTEEQKLVDETRKILGDK
                     TIQVTATVVRIPVIGGHSEAVNVEFEKEYELDDVRKLISSFPGVVLYDNPDEHKYPMP
                     ILAHNRDEVFVGRLRRDHSREKCLNLWIVADNIRKGAATNAIQIAEYMLANNLY"
     gene            complement(2998..4206)
                     /gene="hflX"
                     /locus_tag="PLG42_06385"
     CDS             complement(2998..4206)
                     /gene="hflX"
                     /locus_tag="PLG42_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOU30870.1"
                     /translation="MISTERRAERAVLVGVIHPGQSDTEVKDYLDELEFLADTAGAVT
                     LKRFIQRIEAPNPRTYIGTGKVLEIAEFVNVNKVDIVIFDDDLSPSQVRNIENSIGCR
                     VLDRTNLILDIFAGRAKTSYARTQVELAQYQYLLPRLTGMWKHLERQRGGIGLRGPGE
                     TEIETDRRRIRKRIAHLKEQLEKIDIQMSVQRKNRSRLVRVALVGYTNVGKSTLMNLL
                     SKSDVFAENKLFATLDTTVRKVVIQNLPFLISDTVGFIRKLPHHLIESFKSTLDEVRE
                     SDILLHVVDISHPGFEEQIQVVNETLKEINSSDKPIILVFNKTDIFTYIKKDDDDLTP
                     VTKENYSLEDLKRTWMATASNLPVVFISAKEKHNIDELREILYREVRKIHITRYPYDN
                     FLYNSDHTDN"
     gene            complement(4242..5390)
                     /locus_tag="PLG42_06390"
     CDS             complement(4242..5390)
                     /locus_tag="PLG42_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30871.1"
                     /translation="MKKIIPLLITALLFAGCASSKKHLVKGNYDAAIEKAVKQLRKDP
                     DDKNQKAILEDAYKIANEQDNERIRFLKMEGKPDNWDEIYLIYKRMYDRQALVRTVPN
                     LNMPYVDYMQEMVAAKRKAADYYYAHGMELMKNQTKESYRKAYAEFVRAKEYAGDYEG
                     IDEKIMESKYLGMSRVLVSVQNKSIIRFPKEFEDDLLALNLPALNSEWVEYHTQVLDP
                     NIQFDYLVNVNIINVMVSPDQTVQRDSVIKKEIEDGFTYVLDKKGNVMKDSLGNDIKM
                     PKYKTVQCALIETIQRKSCRIEGDVEILQLNPSKVLKKDPIGAQSDFEHISARALGDL
                     QALSPQQLERTKSQPVPFPSDIDMVIRCSESLKMAIRGAIMNNRRFIN"
     gene            complement(5477..6268)
                     /locus_tag="PLG42_06395"
     CDS             complement(5477..6268)
                     /locus_tag="PLG42_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOU30872.1"
                     /translation="MGRRREIMSFDEIVFENRNKEYGAYILRKKYNSLLIKALIVTMI
                     ISAAAVVIPYYLLLNKTYKGGAGSGRKFVAVSMENIAPPPREIIVPPDIPPPPAPPQS
                     VVKYTAPVIVDSVTNDKTTLPTTDEARNLENKDGEAIAIAEGSGGDELFGEGRGDTPY
                     DFFILEEPPKFRGGDLDKFREWLQRRIIYPPEAQEKGIQGKVILTFVVERDGTVTNVN
                     IVKGVHKLLDEEAKRAIESSPQWTPGMQKGRPVRVRFKIDLNFVL"
BASE COUNT         1926 a         1430 c         1301 g         2124 t
ORIGIN      
        1 ccccgacgcc actgataatg atgaaatgtc aattatttta tccgaaacat tataaaactc
       61 aataaaatcg gcatcacccg gaagaggatt aaagagaagt tcattgaaaa caacatctct
      121 tgttgtaaca ggttcgggaa gtccgaaaat aaaagaattt tttgatgctc tgtttcctgc
      181 gtaatccgta acatcatcga gaagagtaag cgtgtactgc ctgcgcctga caaaaggctc
      241 cgccgaaaca attataaatt ccctttctaa gggatccaaa gcttgaatag aatttacaac
      301 ttttccatta attctaactg cttcacgtaa cctttcaaag tttattacag gttctgaaaa
      361 agagacttta atcataaaag aatcagctgg aaacacattg gtgattcctt caaaatacct
      421 gtcggtataa ctacctgaaa cggaattcac cctaccgggc gtacctcctg atggtgaaac
      481 tgatgctttc cagtttcctt cataattgaa aggacagctt atgtctatca tttcaagcga
      541 ccagccacct tcagctttca gtctatctcc ataccaggaa gaattgtatt caatgccgtg
      601 aatcataact ccggagctgt cggctatcca taagcattta cctgaatcag tcagtgcagg
      661 aaaatattta agacccacca ctgccccata ttgtttgaat aaacctgtat cggcaacaga
      721 acataaaata atataatctc cattatttat tttaacatca gggaaataag atttctgccc
      781 ttcagtgcaa agtgcccagc cttcaagtga aattggctgt tccgaccgcg atgtgatttc
      841 cagatattct ttaaccggca gtgacacggc cggtgtagga tcagccatta tttcagatat
      901 tactacatcg ccgtgctctg cccataatgg gataaaactg attgcaaggt cagagatgca
      961 gtttgaatct ctgtcacaca agttttttat caataactct gatggctgcc ggttgataaa
     1021 gtcagaatca aaatttagaa ggtacgataa agtgcctgtg ctttgtgcat catctacagg
     1081 ataaaaggta ctattcagga gaaaatttga cctgtttaaa aacccgggca atattttttc
     1141 gttaaaaatc agttcaatag ttcttctgct ttttatagtg cagcttacag gttgtggtgg
     1201 ttctgtatcc tgaaagaaag gaccatctat tataagatta tcaaaccata aaagtctgtc
     1261 gcgggttgat gtatatcggt aaaatattcc gaaccattcc acttcaaata tctcattatc
     1321 aatgcccttg tatacacctt tctcttcccc tccaggcaat ttgattttaa tactccacat
     1381 tccatcaggg gtgcgctcaa taaaaattgt tgcaggttgt gtggttccaa tgattgcctg
     1441 ccagttaata tctgttttca ccactgtaac tgccgaacca ttttttattt tccacaccct
     1501 cagagtatca tcataacctg taaaattaac tcctgcggcg tacccgttga cttttccgcc
     1561 cggcttcatt tcgctggcag gggcatccga cataagaaat acggaccagt tattagaaga
     1621 tgatgggtcg cagccatgac gtacctgaaa agaccattta atagttccac gatctggttt
     1681 cagattcata agtctgacag cagcctgtgc tgatcctgct tcagagttat cgaaacaatg
     1741 ccataatgag aatctgcccg acaacggagt aaccgaatca atcccccatt gaccggccgg
     1801 actctggatc cagccttgca aacctgatat ttcaaaatca ttataaacct gaccaaatgc
     1861 agccagtggt agcataacca gaaaagaaac aagaattttc atgcagcgcg acagaatgaa
     1921 aaaaaaaact atttttgacc ttttatttct gaaataaata tagcataatt tatttaataa
     1981 tttaaaatta tcatggtaaa aactgcagtt ataggtgcaa ccggtatggt tggtcgagta
     2041 atggtaaaat tacttgaaga gagaaatttt cctgtttcgg aattaatcct tgcagcctca
     2101 gataaatcag ttggcaaaga gataattttc aaaggaaagc ctgttaaagt tataagcgtg
     2161 aagcaggcag ttgagtcaaa accggttttt gccatttttt cagccggagc agatgtatcg
     2221 aaagagtggg caccagtttt tgcaaataat ggaactgttg tggtcgacaa ctcatctgcc
     2281 tggaggatgg atgacaatat tcctcttgtt gtacccgaaa taaacagtca tgttataaaa
     2341 aaaggtgaca ggataattgc taatcccaac tgttcgacca ttcagatggt aatggcactt
     2401 gcaccattgc ataaagaata caaaatcaaa agaatagttg ttgcaacata tcagtcggtt
     2461 accggaacag gagtcagggc cgtcacccag cttgaaaacg aacgaaaagg tatcaagggc
     2521 gaaatggctt acccgcatcc tattgacctt aactgcttcc cattcggtgg aaaattcctg
     2581 ccatcaggtt atacaactga agagcagaaa ctggttgatg aaacacgtaa aattctgggt
     2641 gataaaacaa tacaggtaac agctaccgta gttcgcatac ctgttatagg cggccattcc
     2701 gaagcagtaa atgttgaatt tgaaaaagaa tatgagcttg atgatgttag aaagctgata
     2761 agcagctttc ccggtgtggt attatatgat aatcctgatg aacataaata tcctatgcca
     2821 atactggcac acaaccgcga tgaggttttt gttggcaggc tcagaagaga tcattcacgt
     2881 gaaaaatgcc ttaatctctg gatagttgca gacaatataa gaaaaggtgc tgctacaaat
     2941 gcgattcaga tagcagagta tatgctggca aataatctgt attgacaggt gaataaatta
     3001 attatcggtg tggtctgaat tatataaaaa attgtcgtag gggtacctgg ttatgtgaat
     3061 tttccttacc tcccggtaaa gaatttccct gagctcgtca atgttgtgtt tttcttttgc
     3121 cgaaataaat accacaggta agttgcttgc ggttgccatc catgtccttt tcaggtcttc
     3181 aagggaataa ttttcttttg taacaggggt aaggtcgtca tcgtctttct ttatataagt
     3241 gaagatgtct gttttattga acacaagtat tataggttta tctgaagagt ttatctcctt
     3301 aagggtctca tttaccacct gtatctgttc ctcgaaaccc gggtgtgata tatccacaac
     3361 atgaagaagt atatcagatt ctcttacttc atcgagggtt gatttgaatg attctataag
     3421 atgatgtggc agttttctaa taaagcctac agtatcagaa atcaggaacg gcaggttctg
     3481 tataacaact tttcttacag tagtatcgag tgtggcgaac agtttgtttt cagcaaaaac
     3541 atccgattta ctcaacagat tcatcagtgt tgatttacca acgttagtat agcctacgag
     3601 tgcaaccctg acaagcctgc tacggttttt acgctgaaca gacatttgaa tatctatctt
     3661 ttcaagctgt tctttcagat gggctattct tttccttatc cttcgcctgt cggtttcgat
     3721 ttctgtctca cccggtcctc tcaggcctat acctcccctc tggcgttcaa ggtgtttcca
     3781 catgccagtc agacggggta aaaggtactg gtattgtgcc agttcaacct gtgtacgtgc
     3841 atatgatgtc ttggctctgc cggcgaaaat atcaagaatc agatttgttc tgtcgagaac
     3901 cctgcaccca atactatttt caatgttacg tacctggctg ggtgagaggt cgtcatcaaa
     3961 aattactata tcaaccttgt ttacattaac aaattcggct atttccagaa cttttcccgt
     4021 gccaatatat gtgcgggggt tgggtgcctc tatccgttga atgaaccttt tcaatgtaac
     4081 agctcctgct gtatcggcaa gaaattcaag ttcatcaagg taatctttaa cttcagtatc
     4141 actttgtcca gggtgaataa cccctaccag aacagctctt tctgccctcc tctcagtact
     4201 aatcattttc tttccccggg ctgtttttta tttctgaaat atcaattaat aaatcttctg
     4261 ttattcataa ttgctcccct gatagccatc ttcagagatt ctgagcatct tatcaccatg
     4321 tcgatatctg aagggaaagg aacaggttgt gattttgttc tttcgagctg ctgcggtgaa
     4381 agagcctgca aatctccaag tgctcgggca gagatatgtt cgaaatccga ctgtgctcct
     4441 atcggatcct ttttcagaac ctttgaggga ttaagctgaa gaatctccac atctccctca
     4501 atgcggcacg acttcctttg aatagtttcg atcagagcac actgaaccgt tttatatttt
     4561 ggcatcttga tatcatttcc aagagaatcc ttcattacat tgcctttttt atccagcaca
     4621 tatgtaaatc cgtcctcaat ttctttcttt atgaccgaat cacgttgaac agtctgatcc
     4681 gggcttacca taacattgat tatgttcaca tttacaagat aatcaaactg aatattggga
     4741 tcaagaacct gggtgtggta ttcaacccac tctgaattaa gagctggtag atttaatgca
     4801 agaaggtcat cttcaaattc ttttgggaat cttataatag atttgttctg aaccgatacc
     4861 agaaccctgc tcataccaag gtattttgac tccataatct tctcgtcgat tccttcataa
     4921 tcgccggcat actcttttgc cctcacaaat tcagcataag ccttacgata agattctttt
     4981 gtctggtttt tcatgagttc cataccatgg gcataataat aatctgcggc ttttcgtttt
     5041 gcagcaacca tttcctgcat atagtcgaca taaggcatgt tcagatttgg aacagtacgt
     5101 acaagtgcct gcctgtcgta catacgtttg taaataaggt aaatttcgtc ccagttatct
     5161 ggtttccctt ccattttcag gaatcttatt ctttcattat cctgttcatt ggcaattttg
     5221 tatgcatctt caagaattgc tttctggttc ttatcatcag gatctttgcg aagctgcttt
     5281 acggcttttt ctatagcagc atcatagttt ccttttacaa gatgcttttt agaggatgca
     5341 caccctgcaa ataacagagc tgtaattaaa agaggtataa tttttttcat gatttataaa
     5401 tgtatattct gaatataaat ttaataaaat gattttaaca aatgtgttgt taaaaaatta
     5461 attatcattt gagtgatcat agcacaaaat tcaggtcaat tttaaatctc accctcaccg
     5521 gcctgccctt ctgcatacct ggagtccact ggggagagga ctcgatggct cgtttggctt
     5581 cttcgtcgag aagtttgtga actcctttaa cgatatttac gtttgtcact gttccgtcac
     5641 gttcgactac aaatgtcagg attacctttc cctgtattcc tttttcctgg gcctcaggag
     5701 gataaatgat tctccgctgc agccattccc tgaatttgtc aaggtctcca cctctgaatt
     5761 tgggaggctc ttcaagtatg aagaaatcat acggtgtatc tccccttccc tctccgaaaa
     5821 gttcatcccc gcctgagcct tcagctatgg ctatcgcttc cccatcctta ttctccagat
     5881 ttctggcttc atctgttgtt ggaagagttg ttttatcatt ggtaacacta tcaacaataa
     5941 cgggcgcagt gtattttaca accgactggg gtggtgccgg tggcggaggt atatcaggag
     6001 gaactattat ttcacgggga ggaggagcaa tattttccat actcaccgca acaaattttc
     6061 tgcctgaacc ggctccaccc ttatatgttt tgttgagcaa taaataataa ggaataacca
     6121 cggcagcagc agatattatc atagttacaa tcaaggcctt aattaacaat gaattgtact
     6181 tcttccggag tatatatgcg ccgtattcct tatttctgtt ttcaaagaca atctcgtcaa
     6241 aactcattat ttccctacgt cttcccattt attcactgtc ttcctttctt cagactcttt
     6301 ttattaaata acagattaaa tttcttacca atttttatgc cagacaaata attttgctct
     6361 aaataaaaac ggttaaaacc ttaattctga tacaactccg caaaactaac aaaataatgg
     6421 attttttctt tttttatttt tttgtacttt tgccgtgccc gaaagggtat atagctcacc
     6481 ctgatagtcc cgatagttat cgggatcgtg aggcttgagc ttcctgacct caggttggga
     6541 aggccagggg ttaattaata ttgttctctt tttcggggat atagctcatc ccgatgacta
     6601 tcgggaggct agagcgtccc gacctcaggt cgggaaggcc aggggttaat taatattgtt
     6661 ctctttttcg gggatatagc tcatcccgat gactatcggg aggctagagc gtcccgacct
     6721 caggtcggga aggccagggg ttaattaata ttgttctctt tttcggggat atagctcatc
     6781 c
//
LOCUS       DAOSNB010000130         6772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11321_length_6772_cov_3.906804, whole genome shotgun sequence.
ACCESSION   DAOSNB010000130 DAOSNB010000000
VERSION     DAOSNB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11321_length_6772_cov_3.906804"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLG42_06400"
     CDS             <1..393
                     /locus_tag="PLG42_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOU30873.1"
                     /translation="FDDSKLLSQLSEAASGNLPGMNSFAAQAPVQIVVIREKPNLLSK
                     IGSTIKDKDYSLIDIGIAAAQICLQAAAEGLGSCMLGWFDEKKVKKILGIPASKRVEL
                     IITIGYPAGPTREKKRKPPEEVISYNRY"
     gene            complement(395..1591)
                     /locus_tag="PLG42_06405"
     CDS             complement(395..1591)
                     /locus_tag="PLG42_06405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU30874.1"
                     /translation="MKEYISDRVMALAESQTLAMAQKSRELKAQGLDIIDLSLGEPDF
                     YTPSDIKEAAKKAIDDNYTKYPPVPGYKDLREAISRKFLEENGLRYSPEQIIVSAGGK
                     HSLANVILSLVNPGEEVIIPAPYWVSYYDQVVLAGGKPVVIHTSLEDDFKMSPAQLEQ
                     AISARTRLLIFNSPSNPTGMVYTRDEMEKIAKVIEKHEDLFVISDEIYEHIIFSQKHV
                     SMASFDFIYDRVITVNGVSKAYAMTGWRIGYIGAPLWIAKACNKLQSQITSGVCTIAQ
                     RAALAALQSRGDDREKMRLAFMRRRDLICSLLAEIKGMKVRIPQGAFYVMPDIGYFIG
                     KTDGNTIINNPDDLALYLLGKARVAVVGGTAFGAPTCIRISYAASDEDITEAVKRMKE
                     ALAELR"
     gene            1896..2420
                     /locus_tag="PLG42_06410"
     CDS             1896..2420
                     /locus_tag="PLG42_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30875.1"
                     /translation="MEILRDILLVTIPSLIVFFTAYFLLRNLIKNDQDKRKQEIILQS
                     NRTITPIRLQAYERLVLFLERISLESLIIRVHSPGMTAAQLQANLLTTIRSEFEHNLS
                     QQIYVSPQAWEVVKNARSNIVKLINTEFEKLPENATGIDFSRRLLEKVMELEKEPTKA
                     AVEFVKSEAARLIM"
     gene            complement(2421..4466)
                     /gene="htpG"
                     /locus_tag="PLG42_06415"
     CDS             complement(2421..4466)
                     /gene="htpG"
                     /locus_tag="PLG42_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOU30876.1"
                     /translation="MQKGKIGVTTENIFPIIKKFLYSDHEIFLRELISNAVDATQKLK
                     VLYNAGEFKQDPGDLTIRVKLDKKKKTLTVSDSGVGMTAEELDKYLNQIAFSSANDFL
                     AKYKDQANNLIGHFGLGFYSSFMVADKVEVITRSWQEGAQAVKWTCDGSPEFTIEEAE
                     RASRGTDVILYIDNDSKEFLEEDRIEEILKKYCKFLPIEIAFGKEKEWKDGKYVETDR
                     DRIINNTKPAWTRKPAELKDEDYLNFYRELYPGSEEPMFYIHLNVDYPFKLTGILYFP
                     RIKSNFDIQKNKIQLYCNQVFVTDSVEGIVPEFMTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLQEIFNKNREEFEKKWDDLKLFISYGMITDEKFYEKASKFA
                     LFKNTDGKYFTFDEYEKQIKEKQTDKFKTLVYLYSTDKHDQYAYIEKAKEKGYDVLLL
                     DGQLDLHLINFLEGKFKNSRFARVDADVIDKLIQKGEEKKSKLTQEQMDDLKAVFKGQ
                     IKPDENFVVVFESLDETDAPVLITRSEYLRRMKDMSQVGGVFSFYGDMPDSYSLVLNG
                     NHPLITRISDDVSKMLGEFLKQNSEEKSKLKEQIEFLEKEHKNKKEEEIRQFEKDDLK
                     KMKEELEKKEKERNDKLAEYSSGNKLVRQLIDLALLANNMLKGEDLSRFVKRSVELL"
     gene            complement(4671..5189)
                     /locus_tag="PLG42_06420"
     CDS             complement(4671..5189)
                     /locus_tag="PLG42_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30877.1"
                     /translation="MAASSKTHIFCLDDHRTFAEDLKKRFSDTTRYEVTVSHNEDDLL
                     KLFSGEKSKKVCKIVIIGIHDSKDNYHVTDELISRIRTSNPETGILLVVSPERMEEAS
                     KQLRFNVDSFIPRNSNMVLRVHNAVKKHISEHNLKIYRKRRNISLLVFFIFLAFSLVV
                     LFIARFKFPHYF"
     gene            complement(5216..6043)
                     /locus_tag="PLG42_06425"
     CDS             complement(5216..6043)
                     /locus_tag="PLG42_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30878.1"
                     /translation="MQTVIRGLIVISFIILSYPGIAQDSISTILEKQFLRIRQNIPSE
                     QKLIIVDSIRQTLDSYVASDTVFNHHFSNLRYLGQITSSDSLLKLLTWNVISADGKNK
                     YYCYFIYRADKNKPHVVHFLSRDYQETPPDENAIYQIDNWYGAIYYDVRPFIYEGEKC
                     YILLAIDYGNPYITRKIIETLKFTPEGKPVFGIPCITDGKNRVRRIIFEYAATAVMSM
                     KFEADTLIVFDHLSPFSPEFKNNRQYYGPDFSFDAYYLDKGGLWNLKEDIDIKNKKK"
     gene            complement(6031..>6772)
                     /locus_tag="PLG42_06430"
     CDS             complement(6031..>6772)
                     /locus_tag="PLG42_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU30879.1"
                     /translation="LLPGVSHIPFNNISSLDIIDGKTACVIVEPVQAEAGVIYPEDGF
                     LSELRNRCTETGSLLIFDEAQTAFGRLGEMFAFQKFGVIPDILVLAKALGGGMPLGAF
                     IASKEIMSSLSHHPALGHITTFGGHPVCCAAGLASLEVIIDEKLIDHVGEKSALFREY
                     LHHKAVKEIRGDGLLLAVETGDREMTSFIISHAPEYGLILDYFLFSPTAFRIAPPLVI
                     TKEEIKEACEKLLALIETAYKNTSGCKR"
BASE COUNT         1854 a         1448 c         1386 g         2084 t
ORIGIN      
        1 tttgacgata gcaagctact cagccagctc tcggaggcag cctctggcaa tcttccaggc
       61 atgaactcct ttgcagctca ggcaccggtt cagattgtag tgataaggga gaaaccaaac
      121 ctgctatcaa aaataggctc cacaattaaa gacaaagatt actctctgat agatattggc
      181 attgcagccg cgcagatatg tcttcaggca gctgcagaag gacttggctc atgcatgctg
      241 ggatggttcg atgagaaaaa agtgaagaag atactcggaa taccagcatc aaagcgggtc
      301 gaactcatca ttacaatagg ttacccggcc gggcctacac gtgagaagaa gcgaaagcct
      361 cctgaagagg ttatatcgta caacaggtac tgaactacct gagttctgcc agtgcttctt
      421 tcatcctttt caccgcttcg gtaatatcct catcagaagc ggcataagat attcttatgc
      481 acgttggagc accgaacgcc gtacctccga caactgctac acgggctttg ccgagaagat
      541 aaagtgcaag gtcgtcgggg ttattaatga tagtgttacc atcggtttta ccgataaaat
      601 agccaatatc tggcataacg taaaaggccc cctggggtat cctcaccttc attcccttta
      661 tctctgcaag cagtgagcat ataaggtcgc ggcggcgcat aaatgccagt ctcatcttct
      721 ccctgtcgtc acctctgctc tgaagagctg ccagagcagc cctctgtgca atagtacaaa
      781 cacccgaagt aatctgactc tgaagcttgt tgcatgcctt tgcaatccag agcggagcac
      841 ctatgtaacc tatcctccat cccgtcatgg cataagcttt cgacactccg ttgacagtaa
      901 tgacacggtc gtatatgaaa tcaaacgaag ccatgctcac atgcttctgc gaaaagataa
      961 tgtgttcata aatttcatcg gagataacaa aaagatcttc atgcttttcg atcacctttg
     1021 caatcttctc catctcatcg cgggtataaa ccatacctgt ggggttcgag ggtgaattga
     1081 agataagcaa ccgcgttctt gcactgatag cctgctcaag ctgggcggga ctcatcttga
     1141 aatcgtcttc gagagaagta tgaattacca ccggtttacc gcctgcaaga accacctggt
     1201 cgtagtaact cacccagtat ggtgcaggaa tgataacttc ttcaccagga ttaacaagtg
     1261 aaaggataac atttgcaagt gaatgctttc caccagccga tacaattatc tgttcaggcg
     1321 aatagcggag tccgttttct tcgagaaact ttctggatat ggcttcccgc aggtctttat
     1381 atcccggaac aggaggatac tttgtataat tatcgtctat tgccttcttt gctgcctctt
     1441 tgatgtcgga aggagtataa aagtctggct ctcccagact gaggtcaata atatccaatc
     1501 cctgggcttt aagctcgcgg cttttctgcg ccattgccag agtctgcgac tctgccagtg
     1561 ccattactct gtcggaaata tattctttca ttgaaattaa attagttgtt aattttttta
     1621 cgataaaagt actcccctct tcctgtctgc attaaaatct atctggtttg taataaaatg
     1681 ttctcatctc gttcttctga tagagccaat ttcagttaac ttttttaaaa ggctcgtata
     1741 taaaaataat ttttcaatag tcaccactat gtaatcgtca accaaatgta aaaacttaaa
     1801 atcaatttaa aaagcaactg cttttcctta agaaaaatag tttgctttct gaaaagaaag
     1861 agttaggttt gtattgaaat tcaatatgaa cagctatgga aattttacgc gatatccttc
     1921 tggttaccat tccttctctg attgtttttt ttacagcata ttttctgctg aggaatctga
     1981 taaaaaatga tcaggataag aggaagcagg aaataatttt acagagcaac cgtacaataa
     2041 ctccgatcag gcttcaggca tatgagcgat tggttctctt tctcgagagg atttcgctcg
     2101 agtcgctcat tattagagtt cacagtccgg gtatgactgc tgcgcagctg caggctaacc
     2161 tgttaaccac tataagaagt gagtttgagc ataacctgtc gcagcagata tacgtaagtc
     2221 cgcaggcatg ggaagtggtt aagaatgccc gttcgaacat tgtgaagctg attaatactg
     2281 agttcgaaaa gcttcctgaa aatgcaacag gtattgattt cagccgcagg ctgcttgaaa
     2341 aggttatgga gctggagaaa gagcctacaa aagctgcggt tgaattcgta aagagtgaag
     2401 ctgcacgact aataatgtag tcacagcagc tccacactcc tcttcacaaa cctgctcagg
     2461 tcctctcctt tcagcatatt attggcaaga agtgcgaggt ctattaactg ccgtacaagt
     2521 ttattccccg agctatattc agccagtttg tcgttccgtt ccttttcttt cttctcaagt
     2581 tcttccttca tttttttaag gtcatccttc tcgaactgtc ttatctcttc ttccttcttg
     2641 ttcttatgtt ctttctcgag gaactcaatc tgctccttaa gctttgactt ttcctcactg
     2701 ttctgcttca gaaactcacc gagcatcttg ctcacatcat ctgaaattct tgttataaga
     2761 gggtgattac cgtttagaac cagactgtag ctgtcgggca tatcaccgta aaaactgaaa
     2821 acaccaccaa cctgcgacat atccttcatc cttctcaaat actctgagcg ggtaatcagc
     2881 acgggtgcgt cagtctcgtc gagcgattca aaaactacaa caaaattctc atctggttta
     2941 atttgccctt tgaagacagc ctttagatca tccatctgtt cctgggtaag ctttgatttt
     3001 ttctcctcac ccttttgaat gagtttgtct attacatcgg catcgaccct tgcaaacctt
     3061 gaattcttaa actttccttc gaggaaattt atcagatgga gatcgagttg tccatcaagc
     3121 agaaggacat cgtaaccttt ttcctttgcc ttctcgatgt aagcgtactg gtcgtgttta
     3181 tcggtagaat ataggtaaac aagcgtttta aatttatcag tctgcttttc ttttatttgt
     3241 ttttcgtatt catcgaatgt aaaatatttt ccgtcagtgt ttttgaaaag tgcaaacttt
     3301 gatgccttct cgtaaaactt ttcgtcggtg atcatgccat acgaaatgaa gagtttcaga
     3361 tcgtcccatt tcttctcgaa ctcttctctg tttttattaa atatctcctg caggcggtca
     3421 gctacttttt tagttatatg ggatgatatc ttctttacat tggaatcgct ctgcaggtaa
     3481 cttcttgaga cattcagggg aatatcaggt gagtcaataa ctccatgaag gagagtcatg
     3541 aattcaggca caatgccttc aaccgaatca gtcacaaaaa cctgattaca gtagagttgt
     3601 attttgttct tctgtatgtc aaaattgctc tttatcctcg gaaaatagag tattcctgtc
     3661 agtttgaacg gatagtcaac gttgagatga atgtagaaca taggttcttc gctgcccgga
     3721 tatagttccc tgtaaaaatt taaatagtct tcatccttca gttctgctgg ttttcttgtc
     3781 caggcaggtt tggtgttgtt tatgattctg tccctgtctg tttcgacata tttcccgtct
     3841 ttccactctt tttcttttcc gaaagcaatc tctatgggga ggaatttgca gtattttttc
     3901 aggatctcct caatgcgatc ttcctcgagg aattcctttg aatcgttgtc aatatacagt
     3961 attacatcgg taccgcgcga ggctcgttcg gcttcttcaa ttgtgaactc agggcttccg
     4021 tcacacgtcc attttactgc ctgagcacct tcctgccacg atctggttat tacttccacc
     4081 ttatcggcaa ccatgaacga tgaatagaaa ccaagcccga aatgtccgat aaggttgtta
     4141 gcctggtctt tatactttgc aaggaaatcg ttggcactcg aaaaggctat ctggttaagg
     4201 tatttgtcga gttcttcagc tgtcatgccc acaccgctgt ccgatactgt taaagtcttt
     4261 ttctttttat cgagcttaac cctgatagta aggtcaccgg gatcctgttt aaactctccc
     4321 gcattgtaaa gtactttcag cttctgtgtg gcatcaacgg cattggagat aagctccctg
     4381 aggaaaattt catggtcgga ataaagaaac ttcttgataa tggggaaaat gttttcagtg
     4441 gtaactccga tttttccttt ctgcataacg aaagcttttt gggttggaca taaaaatcat
     4501 ccctcttttt gcaaaagata tgccgcagaa aaaaactgac aaatagtcac accagaatct
     4561 ggctcagagc cctggttaca gagcacaggg tacaatatgc gaagcaaagg acagcaaagt
     4621 gggaggacac agcagagagc atcttgcaga tgatagttta atgatgatta tcagaaataa
     4681 tgggggaact taaaacgggc aataaaaaga actaccagag aaaaagcaag aaaaataaaa
     4741 aagacaagaa gtgatatgtt tcttctcttg cgatatattt tcaggttatg ttcgcttatg
     4801 tgctttttga ctgcgttatg taccctcaga accatatttg agttcctggg aataaacgaa
     4861 tcgacattga atctcaactg tttcgaagct tcttccatcc tttcaggact gaccacaagc
     4921 aggatgcctg tttctggatt tgaggttctg attctgctaa taagctcatc agtaacatga
     4981 tagttgtctt ttgagtcgtg tattccgata attacaattt tgcatacttt ttttgatttc
     5041 tcaccggaga agagcttaag cagatcgtct tcattatgtg ataccgttac ctcatatctg
     5101 gttgtgtcag aaaagcgctt ttttaaatcc tctgcaaaag ttctgtggtc gtcgaggcag
     5161 aaaatatgtg ttttactgga agcagccata gtctatcatt tgataatcaa gttactcatt
     5221 ttttcttatt tttaatatca atatcctctt tcagattcca caaccctccc ttatcgagat
     5281 aataggcatc gaatgaaaag tccggaccgt aatactggcg gttattttta aattcaggtg
     5341 aaaacggcga aaggtggtcg aacacaatca gtgtatctgc ttcgaatttc attgacatca
     5401 cagcagttgc tgcatattca aagataatcc gccttactct attcttacca tcagtgatac
     5461 atggtattcc gaaaacaggt ttgccttcag gagtaaattt cagtgtttca ataatttttc
     5521 ttgtaatata aggattgcca tagtcaatgg cgagaagtat atagcatttt tcaccttcat
     5581 aaataaaagg tctcacatcg taatatatag caccatacca gttatcaatc tgatatattg
     5641 cattttcatc aggaggtgtt tcctgataat cacggctcag aaaatgaact acatggggtt
     5701 tgttcttatc tgcccggtag atgaagtaac agtagtattt attttttcca tccgcagaaa
     5761 tgacattcca tgtaagcaat ttcaggagtg aatcggagga agttatttgt ccgagatagc
     5821 gcaagttgga gaaatgatgg ttaaagacgg tatcggaggc gacataggaa tcgagcgttt
     5881 gccttattga atccactatg atcagtttct gttccgacgg aatgttctgc cggattctca
     5941 aaaactgttt ttcaagtatc gttgatatgg aatcctgtgc aatacctgga tatgaaagga
     6001 taataaacga tatgactatc agccctctta ttaccgtttg catcctgatg tgtttttgta
     6061 cgctgtttct atgagtgcaa ggagtttttc gcatgcttct tttatttcct cttttgttat
     6121 gacgagtggt ggtgctattc tgaatgctgt gggggaaaag agaaaataat ccaggatcag
     6181 tccatattcc ggggcatgtg aaatgataaa gctggtcatt tctctgtcac cggtctccac
     6241 agcaagcaga agtccgtcgc ctcttatttc tttaacggct ttgtgatgca gatattctct
     6301 gaatagagca gatttttccc ctacatggtc aatgagtttc tcatctatta tcacttccag
     6361 tgatgcaagt cctgcagcgc agcacacagg gtgtccgccg aaagtagtga tatggccaag
     6421 agcagggtgg tgcgaaagtg atgacataat ttcctttgaa gcgatgaatg cgccaagtgg
     6481 cataccgcct ccaagggctt ttgcaagtac aaggatatcg ggaatgactc cgaatttctg
     6541 aaaagcaaac atctcgccca gtctgccgaa tgctgtttgt gcctcatcaa aaatcagcag
     6601 tgagcctgtt tcggtacagc ggttgcgcag ttcactgaga aagccatctt caggataaat
     6661 gactccggct tcggcctgca cgggttcgac aataacacat gcagtttttc cgtcaataat
     6721 atcgagtgaa gatatattat taaaaggtat atgagaaacc cctggcagta aa
//
LOCUS       DAOSNB010000131         6724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11431_length_6724_cov_4.203479, whole genome shotgun sequence.
ACCESSION   DAOSNB010000131 DAOSNB010000000
VERSION     DAOSNB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11431_length_6724_cov_4.203479"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLG42_06435"
     CDS             <1..740
                     /locus_tag="PLG42_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOU30880.1"
                     /translation="SINKCGLKVIAHRGAWKKNGHPQNSIASLRNAIELKVDGSEFDV
                     RITSDDSLVINHDREYNKMVIEKTPYTTLVKYRLSNGEKLPTLREYIAEGLKNNSTTK
                     LICELKSAEAGRERNIYMAEKSIMLAKELNALNYITWISFDYDIVKRISELLPGAEVF
                     YLNGDIAPIELKKASIKGAGYNYSVFRKRPEWIEEAKKEGILLNVWTVNNEDDMDFFI
                     SKCFDYITSDEPEILMQKIKEYRQSKR"
     gene            818..1717
                     /gene="rbsK"
                     /locus_tag="PLG42_06440"
     CDS             818..1717
                     /gene="rbsK"
                     /locus_tag="PLG42_06440"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOU30881.1"
                     /translation="MKEILVIGSSNTDMVIKTEKLPSPGETILGGTFLLNPGGKGANQ
                     AVAATRLGGKVTFVGKRGNDLFGNQTVGLLMREGIDTEFFIKDPELPSGVALITVDSN
                     GENMIVVAPGANANLQPEDLPEEIFDSSRYAILLIQLEIPITTVEYAIKKAKEKGIKA
                     ILNPAPAQPLSDEILKNVWALTPNEVEAEALTGVSISDTKTCSRAAAVLRNKGVRNVI
                     VTLGEGGAFVSSDLYSGPIPGIKVDAVDTTAAGDVFNGALAVALAEGKELPEAVKFAN
                     KAASISVTRLGAQASAPFRHELD"
     gene            1793..2725
                     /locus_tag="PLG42_06445"
     CDS             1793..2725
                     /locus_tag="PLG42_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30882.1"
                     /translation="MKKLLFFPVLFLITISTITAQFVGAPPEYIKAITPEWKGERFPD
                     GRPRVPDALLERLKNVSIEEAWGYLRNRGYMNQFEGDWIVLHPDQPMTGRVVTAQYMP
                     LRPDLEKVIRERGKVEGRTQQGGTNSWPIDVLVEGDVYVADSYGKIVDGTLIGDNLGN
                     SIYAKSKRGVIFYGSVRDQEGLEEIEGFNGWIKGSDPSFIQQMSLVTINTPIRIGRAT
                     VLPGDVVLAKKYGVIFIPAHLVADLIINAEVTALRDEFGHQRLREGKYKPGQIDSAWS
                     DEIKKDFMEWLSKYPGKLPMSKQELDNYFKERNW"
     gene            2864..4339
                     /locus_tag="PLG42_06450"
     CDS             2864..4339
                     /locus_tag="PLG42_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOU30883.1"
                     /translation="MKSIIEKIRREDQLSGYAQAGDKQSHLQTGSPSTSRRDFLRKTA
                     LGGIALGGFMTLPLEDTIAQATSKVNRASYPSDLKITDMRVATVANHHIIKIYTNQDI
                     YGLGEVRDGADARYALFLKSRILGLNPCNVEMIFKIIRQFGYHGRQGGGLSAVEMALW
                     DLTGKAYNVPVWQLLGGRYRDKIRLYADTPGVQDPKEFAEKVRLRMEVQGFTWLKMDL
                     GIHLVAKKEGNIINSKYWAPLAQYDLRDYMGYGNTLHSFTQVQITDQGLADLQNYVAM
                     VRDAVGYEIPLCADHFGHFDVNNAIRFGRAMEKFRLAWLEDMVPWFDIENNKVLSQAL
                     ETPILTGEDIYMLNGGFKPLIDARAVDIIHPDLAIAGGILETKKIGDYAEENNIAMAL
                     HMAGSPVCFMANVHCAAATQNFLALEHHSIDTPWWENLVRMTGSKPMIEKGFANVPLD
                     APGLGIELNEEECKKRLGKEYGWFNPTPEWDQKRSHDRLWS"
     gene            4387..5661
                     /locus_tag="PLG42_06455"
     CDS             4387..5661
                     /locus_tag="PLG42_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOU30884.1"
                     /translation="MKTKCLVIIALVFIESVSGFSQINSLLPPEQKLSLLKKACEEEL
                     INNILSWWSKVMPDEVNGGFYGRVDGNNTIIYGAPKGGILNARILWTYSSAYRMTRNP
                     EYLQLATRAKDYILEHFIDREYGGAYYSLNAEGEPLDTRKHTYTQAFFIYGLSEYARA
                     TGDKVALTVALEIFNLLEKHAFDTVAGGYFEAFSRDWSRLRDRMTGERTPADEKTMNT
                     SLHIMEAYANLYRIHPVKRLKEKVRSMVEIFLEKIIDKKTGHLICFFDRSWNATSKID
                     SYGHDIESSWLLYETAQLLNDRKLLEKVRATSIRIADAAMEGITPEGAMLTEKNNETG
                     EVRTTRSWWEQAEAVVGYLNAYELTGNEKYINTAINAWNYINRYFVDRTNGGWYSNVT
                     PEGTGRGDKAGHWICPYHNGRMCLEVMGRIKK"
     gene            complement(5680..>6724)
                     /locus_tag="PLG42_06460"
     CDS             complement(5680..>6724)
                     /locus_tag="PLG42_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOU30885.1"
                     /translation="VPLLQRIMFGMGSQMSAKDFAGIIKMPKGVIIGIFLQFTIMPLV
                     GLTIANIFNFPAEIAAGIILIGCVPSGLASNVMSFLARANLALAVTLSSVTTVLSPLV
                     TPFLMKTLAGQFIEVNFWSMMLDILNMIILPVVAGFTFNLVAKRSESHKSAVIQIIIF
                     ISVILVTTLVYTKSTNTGFSGFVVRFLKSLGWFMLLPAAGALILSKYLKGDSRIMEQV
                     LSFFSMAGIAMIITIITASGRESLLHVGLLLLLTSLLHNLTGYTLGYFFAWLTGMPER
                     DRRTIAFEVGMQNGGLASGLALQMGKLATVGLAPAIFGPLMNITGSILANWWRGKPVK
                     DEDEVNVEPTKEK"
BASE COUNT         2086 a         1380 c         1554 g         1704 t
ORIGIN      
        1 attcaattaa taagtgcgga ttaaaagtaa ttgcacatcg tggtgcctgg aagaaaaacg
       61 gccatcctca gaactccata gcttcacttc gtaatgctat tgaacttaaa gttgatggtt
      121 ctgaatttga tgtcaggatt acttctgacg actctctcgt gataaatcac gacagagaat
      181 ataacaagat ggtaattgag aaaacacctt atactactct tgttaaatac aggctttcta
      241 atggagaaaa attgccaact ctgcgggaat atatagccga aggattaaaa aacaacagca
      301 caacaaaact tatatgcgag cttaaatcgg cggaagctgg cagggaaaga aatatttaca
      361 tggcagaaaa gagcatcatg cttgcaaagg agctgaatgc tcttaattat attacttgga
      421 taagttttga ttatgacatt gtaaaaagga tttcggaact tctgccgggg gcggaagttt
      481 tttatcttaa tggcgacatt gcaccaattg aattaaaaaa ggcatcaata aaaggtgcag
      541 ggtataatta cagcgttttc aggaaaagac ccgaatggat tgaagaggca aagaaagaag
      601 gtattcttct taacgtctgg acagttaaca atgaagacga tatggatttt ttcatctcaa
      661 aatgttttga ttatatcaca tccgacgagc ctgaaatatt gatgcagaaa ataaaagaat
      721 accgccagtc aaaaagatga aggttatctg tatcggaagg aaattttaat tttgtaaaag
      781 ggacgttatt attaacttct ttattaaaaa ttattatatg aaagaaatcc tcgtaatcgg
      841 cagttccaat actgatatgg ttatcaagac ggagaagctt ccatcgcccg gggaaacgat
      901 tctcggaggg acgtttcttc tcaatcccgg aggcaagggt gccaatcagg cagttgcagc
      961 cacaaggctg ggcggaaaag tgacttttgt cggtaaacgg ggaaatgacc tttttggaaa
     1021 ccagactgtt gggttgctga tgagggaagg cattgatacc gagttcttta taaaagatcc
     1081 tgaattacct tcgggtgttg ctctgattac cgttgattcg aatggagaaa acatgatagt
     1141 tgttgctcct ggagcaaatg ccaatctgca gcctgaagac cttcctgaag aaatcttcga
     1201 cagcagcaga tacgctatac ttttgataca gcttgaaata cccataacta ctgtggagta
     1261 tgccatcaaa aaagcaaagg aaaaaggaat caaagctata cttaaccctg ctcctgcaca
     1321 gccactttca gatgaaattc tgaaaaatgt atgggctctg actcctaatg aagttgaggc
     1381 tgaggcactc acgggtgttt cgatttccga cacaaaaaca tgttccagag ctgctgcagt
     1441 tttaaggaat aaaggagtta gaaatgtgat tgttaccctg ggtgagggag gtgcttttgt
     1501 gtcatctgat ttatattcag gtcctatacc aggcattaag gttgatgctg tagacacaac
     1561 cgcagccggt gatgttttca atggtgctct tgcagtggct cttgccgagg gaaaagaact
     1621 tcccgaagct gttaagttcg caaataaagc tgcttcaatt tctgtaaccc gtcttggagc
     1681 acaggcatca gctcctttca gacacgaact cgactgaacg aagaaatcaa cttcacttaa
     1741 tcgcctgaat tttttatctt taacttaaca aataattata aaaccataca gcatgaaaaa
     1801 gcttctcttt tttccggtat tatttctaat tacaatcagt acaattactg cccagtttgt
     1861 tggcgctcct cctgagtata taaaagcaat cacacccgaa tggaagggag aacgttttcc
     1921 tgatggcaga cccagagtgc cagatgctct tcttgagcgt cttaaaaacg tatcgataga
     1981 agaagcctgg ggttacctgc gcaacagggg atatatgaac cagtttgagg gagactggat
     2041 tgttctgcac cccgaccagc ctatgacagg gcgggttgtt acagcccagt atatgccttt
     2101 gcgtcccgac ctcgaaaagg taatacgtga acgaggcaag gttgaaggac gaacccagca
     2161 gggtggaaca aactcatggc ctattgatgt gctcgttgaa ggagacgtat acgttgccga
     2221 cagttacggt aaaattgtcg atggcacact gataggtgac aatcttggta attccattta
     2281 tgcaaaatca aaaagaggag tcatatttta cggttccgta agagaccagg aagggttaga
     2341 ggaaatcgaa gggttcaatg gctggatcaa aggctccgac ccgtcattca tccagcagat
     2401 gtctcttgtt acaattaaca ctccaatacg cattggaaga gccacggttt taccaggtga
     2461 tgtagtactg gcaaaaaaat acggagtcat ttttataccg gcgcaccttg ttgccgacct
     2521 gataattaat gctgaagtta ctgccctcag agacgaattc ggacaccagc gtctgcgcga
     2581 aggaaaatat aaacccgggc agattgacag cgcctggagc gatgagataa aaaaggattt
     2641 tatggagtgg cttagcaaat acccgggaaa attgcctatg tcgaaacagg aactcgataa
     2701 ttattttaag gaaagaaact ggtagagtca tcacagctgg agatagagat ctggtaatca
     2761 gttaaatgaa tgatactgca aattcagatg ccatagtctc ttaacgaaga ctttcgtcat
     2821 tttcgcggtc aaaaacgcta ttaaaagcat aaagttctat tatatgaaat ctattatcga
     2881 aaaaatcaga agagaagacc agctttcagg gtatgcacaa gctggtgata aacaatcgca
     2941 tcttcaaaca ggttctccat caaccagcag gcgtgatttc ctcagaaaga cagcacttgg
     3001 gggaattgcc cttggcgggt ttatgacttt accgcttgaa gataccatag cacaggcaac
     3061 atcaaaggtc aacagggctt cttatccttc ggatctgaaa ataactgaca tgagggttgc
     3121 aactgtcgcc aaccatcata tcataaagat atacaccaat caggacatct atggtcttgg
     3181 tgaggtacgc gatggagccg atgcccgtta tgcactcttt ctgaagagcc gtatcctggg
     3241 gcttaatcca tgtaatgtcg aaatgatttt caaaataata cgccagttcg gctaccacgg
     3301 gcgacagggt ggcggcttaa gtgcagttga aatggcttta tgggatctta caggtaaagc
     3361 atataatgtt ccggtatggc aattgcttgg aggcaggtac cgggataaaa tacgccttta
     3421 tgcagatacc ccaggcgttc aggatccgaa agagtttgct gaaaaagtaa ggctgagaat
     3481 ggaagtgcag ggcttcacat ggctaaaaat ggacctcggg atacatcttg tggctaaaaa
     3541 ggaaggaaat atcatcaaca gcaaatactg ggcaccgttg gcacagtacg accttcgcga
     3601 ttatatgggc tatggcaata cactgcattc cttcacacag gttcagataa ccgaccaggg
     3661 gcttgcagat ctgcaaaact atgtagcaat ggtgagagat gcagttggct atgagatacc
     3721 actatgtgcc gatcatttcg gccacttcga cgtaaacaat gccatacggt ttggaagggc
     3781 aatggagaaa ttccgtctgg catggctcga ggatatggtg ccatggttcg atatcgagaa
     3841 caacaaggtg ctgtcacaag cccttgaaac ccctatattg acaggcgaag acatctatat
     3901 gctcaatggc ggtttcaaac ctcttattga tgcccgtgcc gttgatataa tccatcccga
     3961 tctggcaata gcaggaggaa tactcgaaac caagaagatt ggagattatg ccgaagagaa
     4021 taatattgcc atggcactcc atatggcagg gtcgccagta tgttttatgg caaatgtcca
     4081 ttgtgctgct gcaacacaga actttctggc tcttgaacac catagtatag acacaccgtg
     4141 gtgggagaat ctcgtcagaa tgaccggctc aaaacctatg attgaaaaag gctttgccaa
     4201 tgtcccgctc gatgcaccag gactcgggat tgaactgaac gaagaggaat gcaaaaagcg
     4261 cctcggcaaa gaatacggat ggtttaaccc caccccggaa tgggaccaga agcgttcaca
     4321 cgaccggctc tggagctaaa actatatgct acttcgtatt gtctttcgtg gttaaacaga
     4381 ataaaaatga aaactaaatg tttagttata atagcactgg tatttattga gtctgtttca
     4441 ggcttttccc agattaactc tttacttcct ccggagcaaa agctttcatt gctcaagaaa
     4501 gcatgcgaag aagaacttat caacaacatc ctatcgtggt ggtcgaaagt gatgcccgat
     4561 gaagttaacg gtggtttcta cggacgggta gatggcaaca acacaataat ttacggtgca
     4621 ccaaaaggag gtattctgaa tgcccgtata ctctggacat attcctcagc ttacagaatg
     4681 accagaaacc ccgagtacct acagctggca acccgtgcaa aagattacat tctggagcac
     4741 tttatcgaca gggaatacgg cggtgcatat tacagcctca atgccgaagg tgagcctctc
     4801 gacacacgaa aacatactta tacacaggca ttttttatat acggactttc agaatatgct
     4861 cgtgcaaccg gtgataaagt ggctttgaca gttgctttgg aaattttcaa tctccttgaa
     4921 aaacatgcct tcgatactgt agcaggcggt tatttcgaag ccttcagccg cgactggtca
     4981 cgtttgcgcg acagaatgac cggtgaaaga acgccagccg acgaaaaaac aatgaacact
     5041 tcacttcaca taatggaagc gtatgcaaat ctttacagga tacatcctgt gaaacgcctg
     5101 aaggaaaaag taagaagcat ggttgaaatc ttccttgaaa aaattataga caaaaaaacc
     5161 ggccatctca tatgtttctt cgacagaagc tggaacgcca cttcgaagat tgattcatac
     5221 ggacatgaca tagagtcgtc atggctgcta tatgagaccg cacaacttct caacgacagg
     5281 aaacttctcg aaaaggtcag ggcaacgagc atcagaattg ctgatgcagc catggaaggg
     5341 attacgcctg aaggggcaat gcttactgaa aaaaacaacg aaacaggtga agttagaact
     5401 acaagaagct ggtgggaaca ggcagaggca gttgtgggct atttaaatgc ctacgaactc
     5461 accgggaatg aaaagtatat caacactgcc atcaatgcat ggaattacat caacaggtat
     5521 tttgttgaca gaacaaacgg aggctggtac agtaatgtca cacctgaagg gacagggagg
     5581 ggtgataagg caggccactg gatatgtccc tatcacaatg gaagaatgtg ccttgaggta
     5641 atgggaagaa ttaaaaagta aaacaggagc aggttctttt tatttttctt ttgtcggttc
     5701 tacatttacc tcatcttcat ccttaaccgg ttttcctctc caccagtttg caagtattga
     5761 tcctgtaata ttcatcagcg gcccgaaaat agccggggca agtcctaccg ttgcaagttt
     5821 ccccatctga agtgccagcc ccgatgccag cccaccgttc tgcataccga cttcgaaggc
     5881 gatagtgcgg cggtctcttt cgggcatgcc tgtcagccat gcaaaaaagt atcccagtgt
     5941 gtaaccggtg agattatgta agaggctggt taacagcaga agcagcccga catgcagcag
     6001 gctctccctt cctgatgcgg taataattgt tattatcatc gctattcctg ccatggagaa
     6061 gaatgagagt acctgttcca ttatcctgct gtcgccttta aggtattttg aaagtattaa
     6121 agcccctgcg gctggcagaa gcatgaacca gcctaatgac ttcagaaatc tgacgacaaa
     6181 tcctgagaaa ccagtatttg tcgattttgt ataaacaaga gttgttacca gtatcacaga
     6241 aataaatata attatctgaa ttacagccga tttatgcgat tcacttcttt ttgccacgag
     6301 gttaaatgta aagccggcca caaccggaag gataatcata ttgaggatgt cgagcatcat
     6361 gctccagaaa ttgacttcaa taaactgacc tgcaagtgtt ttcatcagga agggagttac
     6421 cagcggagag agaaccgtgg taactgaact gagtgtaacc gcaagggcaa ggttggcacg
     6481 tgcaagaaat gacataacgt tagaagcaag gccgctggga acacagccta tgagtattat
     6541 accggcagca atttcggcag gaaagttaaa gatatttgca attgtgagtc ctaccagcgg
     6601 cattattgta aactggagga aaataccaat tataactcct ttcggcattt taattattcc
     6661 tgcgaaatcc tttgcactca tctgcgaacc catgccaaac ataattctct gaagaagggg
     6721 gaca
//
LOCUS       DAOSNB010000132         6714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11445_length_6714_cov_5.135306, whole genome shotgun sequence.
ACCESSION   DAOSNB010000132 DAOSNB010000000
VERSION     DAOSNB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11445_length_6714_cov_5.135306"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..2015
                     /locus_tag="PLG42_06465"
     CDS             237..2015
                     /locus_tag="PLG42_06465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOU30886.1"
                     /translation="MKKADSESNLIIPAGTIFTDTLLRIYRYNTLNKIYSETQNKDPL
                     TLIESLIKYLDIKIDIPETDLKNIPASGPFIAVSNHPYRGIDSMILYKLINSRRPDIK
                     ILGSNQLYDIAPLRDAIIPVHTSHESIDYPISSFSGIRKVLSHLKEGKCVGIFPAAED
                     TKQFEAPGVIIDREWGKATVKLIKSAGVPILPVYFHGTLTRIKYIIRNMSPVESKPFL
                     PHELTKGKNRIISVRIGSPISVKEVAAISDLNTFERYLRARVFSLGATIKNKEKLSKA
                     SRKRKSEPVTEPIDPAVLQTEYERIKNNCELFSTKNYSVLCAPAYVIPNIFREIGRLR
                     EVTFREVGEGTGKSIDIDKYDFYYQHLFIWDNDEQRLVGAYRLGKGKDIMRLYGIKGF
                     YISSLFKIKKPFSGVLSQSIELGRSFIVRDYQKKVMPLFLLWKGIMIFLLKHPDYRYL
                     IGPVSISNDLSKYSKSLLVEFINKWFFDRKLARYIKPRKNFVPVSDNVNVRNVFIDIS
                     EKDINKIENIITDVETGYRMPVLLKKYLEINGKIIGFNIDPKFNNCLDGLMILDVFEA
                     PIELISGLAKEMNDPLVFERFRVRTE"
     gene            complement(2035..4152)
                     /locus_tag="PLG42_06470"
     CDS             complement(2035..4152)
                     /locus_tag="PLG42_06470"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOU30887.1"
                     /translation="MAAIQYLREKAGVFVAILIGLSLFIFVISDFFGGGRSARIRQRK
                     YYEIGQVAGERIMYQDYEQRIQSLQEIYKLSGTTSFDEATLESIREQVWQQMIRERIL
                     DKKFNRLGIGVSTEELDELVLGVNPHPIVTQLFTDRQTGIFNRSFLINFLKATETDET
                     ARKYWLFFENEIINDRANTKFNNLIGKGLYVTSKQADFESNLLSSTVDFSYLVKNYSM
                     VPDTAFKVTAKEIENYYNTHKESYRRTAQRDIEYVAFDIVPSEDDYRQAEEWINRIKE
                     EFASTDDPAQFINTTSDTRHTGYYNTINEIPDTLREFVRKENRKEVFGPWFENGSYKL
                     ARLIDAGDRPDSVHVRHILISPRENITLQKAREIADSLVKVIKSGTPFETVARNNSDD
                     QGSAAIGGDLGWFREGVMILPFNNACFTGNKGEIKVVETTFGVHIIEILDLSKKVRKY
                     NVGIIERKVVPGTLTTQKIYAEASRFAGSASTYDKFNKVIADEKLNKRVATNVTPQQK
                     TLPGLNNPRYLIMSLFQAQPGSIILDANQQAIFEIDDKFVVAYCTRAIEEGPAPLSEV
                     ENDIRYILVRDKKAEIISEELKNHIKSGKTLEDIAALLGTQVMEARQINFNSYTVPGA
                     GVEPALVAAATVSKEGTVSGPVKGNNGIYLLKVNNITRTSDEDLKLLRDRLSLTYRMR
                     GTYEAYDALKKSSVIVDKRYKFY"
     gene            4579..>6714
                     /locus_tag="PLG42_06475"
     CDS             4579..>6714
                     /locus_tag="PLG42_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOU30888.1"
                     /translation="MAELRFNALKEIFNRKPVEIEDFPSNISEIFAVNVFDLPKMERY
                     LPREAYLAVKKSIDTGMPISRQIADQVATGLKAWAIERGATHYTHWFHPLTDATAEKH
                     DAFLERTDGSKMIETFSGELLVQQEPDASSFPSGGIRNTFEARGYTAWDVSSPAFIVG
                     STLCIPTIFVSYTGEALDYKTPLLKALSALDKAAVRVCQYFDKDVTRVYATLGWEQEY
                     FLIDEALYYARPDLMLADRTLMGHQSSKDQQLSDHYFGSIPERVIAFMKEFELEAYKL
                     GIPVKTRHNEVAPNQYECAPIFEEVNLAVDHNQLLMDIMRRVARRHNFRVLFHEKPFA
                     GINGSGKHNNWSMATNTGVNLLSPGRNPKTNLQFLTFLVNVIKAVNDNQELLAASIIN
                     EPNSHRLGGHEAPPAIISVFIGKYLSSVLDEIVSKVTTRKMSPSLKTDLKLGIGKIPE
                     ILLDNTDRNRTSPFAFTGNRFEFRAVGASANCGAAMIVLNTAVARQLEIFSSEVDALI
                     KKGRKKDEAIFQVLKKYIIESQRIRFEGDNYSEEWKKEAARRGLHIITSVPETLKLYL
                     TPQGKSLLVETGIFSERELKSRVDVEYEKFIKKVQIQSRVLGDLALNHIVPVAIDYIT
                     SLLDNVKGLKEVFPEEESENLAAPRKELIKKISYHISEIQRLVHDMIEERKKANVIED
                     LYDKAIAYERNVKPYLEEIREHIDKLERIV"
BASE COUNT         2094 a         1307 c         1296 g         2017 t
ORIGIN      
        1 aggtcgtcgc cgaactttaa aattaatccc gaccctcagg tcgggatttt tttttaccaa
       61 aaaccacccc ggaagagaac gctgaacaaa aaaaatgaag ttcagtcccg attaaccgca
      121 ccgttttttt aactgccctt ctatcctgaa ttaatcagtt tatacaatct aaccctgcat
      181 aaaataactt tattcactaa atttgtgtat tgacttaaat aaatattttt tccggagtga
      241 aaaaagcaga ttcagaatca aacctcataa ttcctgcggg aacgatattt actgataccc
      301 tgctgagaat ttacagatac aatactctta ataaaatcta ttcagaaacc caaaacaaag
      361 atccactgac attaatcgaa tcacttatca aatatcttga cataaaaatt gatattccag
      421 aaactgacct taaaaatatc cctgccagtg gaccatttat tgcagtctca aatcaccctt
      481 acagaggaat tgattcaatg atactctata agctgataaa ttccagaagg cctgacataa
      541 aaattcttgg aagtaatcag ctgtacgata ttgcacctct gcgagatgca attattcctg
      601 tacacacttc tcatgagagt atagattatc caatatcttc tttttcagga ataagaaagg
      661 ttttaagcca ccttaaagag ggtaaatgtg tgggaatctt tccggctgct gaagatacaa
      721 agcaatttga agcacccggt gttattatcg acagggagtg gggtaaggcg acagttaaac
      781 tgatcaaatc agcaggtgtg ccaattctac ctgtttattt tcatggcacc cttaccagga
      841 ttaaatacat tatccgtaac atgtcacctg ttgaaagcaa accattttta ccccatgagt
      901 tgacaaaagg gaaaaaccgg attatttcag taaggattgg ctcacctata tctgtaaaag
      961 aagtagctgc tatttcagat ttaaatacat ttgagagata cctccgggca agggtttttt
     1021 cacttggtgc aacaataaag aataaggaga agctcagtaa ggcaagtcgt aagcggaaat
     1081 cagaacctgt tacagaaccc attgaccctg ccgttctaca gactgaatat gaacgtatta
     1141 aaaacaattg tgaactcttt tcaacaaaga attattctgt tctgtgcgca cctgcatacg
     1201 ttatacccaa tattttcaga gaaataggca gactgagaga agtaaccttc agggaggtgg
     1261 gagaaggaac cggtaaaagt attgatatcg acaaatatga tttttattac cagcatctgt
     1321 ttatctggga taacgatgaa caacggctgg taggtgccta tcggctggga aaagggaaag
     1381 atattatgcg gctttatggc atcaaaggtt tctatataag ttcactgttt aaaattaaaa
     1441 agccattttc cggggtgctt tcacaatcaa tcgaactggg tcgttctttt atagtcagag
     1501 attatcagaa aaaagttatg cccctgtttt tgctgtggaa aggaataatg atatttcttt
     1561 taaaacatcc tgattaccgc tacctgatag gacctgtaag cataagcaac gatctctcga
     1621 aatattcaaa aagcctgctt gtggaattta taaacaaatg gttttttgac aggaaacttg
     1681 ccagatatat aaaacccaga aagaattttg ttcctgtatc cgacaatgtc aatgttcgca
     1741 acgtttttat agatatttct gaaaaggata taaataaaat tgaaaacatt ataaccgacg
     1801 ttgaaacagg ttaccggatg cctgtccttc ttaaaaaata tctcgagata aacggtaaaa
     1861 taataggctt taatattgat ccaaaattca ataattgcct tgacggatta atgattctcg
     1921 atgtttttga agcccccatc gagttaattt ccggtcttgc gaaagaaatg aatgacccct
     1981 tggtgtttga acgcttcagg gtaagaacag aataataagt cctgtgaaat ttttttagta
     2041 gaacttatat ctcttgtcca caataacaga gctctttttc aaagcgtcgt aggcctcata
     2101 agttccgcgc atcctgtaag ttaacgagag tctgtctctt aaaagtttca ggtcttcatc
     2161 tgaggttctg gttatattgt ttaccttcag aagatagatt ccgttatttc ctttcaccgg
     2221 ccctgagaca gtaccttctt tagatactgt tgctgcagca accagagcgg gttcaacacc
     2281 tgcgccgggt acagtataag aattgaaatt gatctgtctg gcttccataa cctgagttcc
     2341 cagaagagct gcaatatcct caagtgtctt ccctgattta atgtgatttt tgagctcctc
     2401 tgaaattatt tcggcttttt tgtctctcac aagaatatat cttatgtcat tctcaacctc
     2461 gctcagagga gctggtcctt cttcaattgc ccttgtgcag tatgcaacaa caaacttatc
     2521 gtcaatttca aaaatagcct gctgatttgc atcaagaatt atactaccgg gttgagcctg
     2581 aaaaagtgac ataataagat agcgcgggtt gttcagcccg ggcaaggtct tctgctgagg
     2641 agtcacattt gtggcaactc ttttgttaag tttttcatca gcaataactt tgttgaattt
     2701 gtcataggta ctggctgaac cggcgaaacg gcttgcttca gcatatattt tctgagttgt
     2761 caatgttccg ggaacaactt ttctctcaat tattccaacg ttgtatttcc tgaccttttt
     2821 tgaaagatcg agaatttcaa tgatatgaac cccaaaggtt gtttctacga cttttatttc
     2881 tcctttgtta cctgtaaaac atgcattatt gaagggcaga atcatgacgc cttctctgaa
     2941 ccagccaagg tctccgccta tggcagcaga accctggtca tcggaattgt tacgtgcaac
     3001 tgtttcaaag ggagttcctg attttataac ctttacaaga ctgtcagcga tctcccgtgc
     3061 cttttgtaaa gtgatgttct ctcttgggga aatcaatata tgcctgacat gaaccgaatc
     3121 gggcctgtcg cctgcatcaa ttagtcgtgc aagcttgtat gatccattct cgaaccaggg
     3181 gccaaatacc tctttacggt tttctttcct gacaaactcc ctcagtgtat ctggaatttc
     3241 attaatggta ttatagtatc ctgtatgcct tgtatcagag gttgtattaa taaattgcgc
     3301 aggatcgtct gttgatgcaa attcttcctt tatcctgttt atccattctt ctgcctgcct
     3361 gtagtcatct tcagaaggga ctatgtcgaa cgcaacatat tcaatatctc tttgtgcagt
     3421 tctcctgtaa ctttccttat gggtgttgta ataattttct atttctttag ccgtcacttt
     3481 gaatgcagtg tcgggaacca ttgaataatt ttttaccaga taactgaaat caacagttga
     3541 tgaaagaaga ttgctttcaa aatcagcctg ctttgaggtc acataaaggc cctttccaat
     3601 gaggttgttg aattttgtat tggctctgtc gttaattatc tcattctcaa aaaataacca
     3661 gtattttctt gcagtttcgt ctgtttcagt tgctttaagg aaattaatca aaaaggagcg
     3721 attgaaaatg cccgtctggc gatcggtaaa aagctgggtt acaataggat gtggattaac
     3781 gcccagcacc agttcatcaa gctcttcagt gcttacgcca ataccgaggc ggttaaattt
     3841 tttatcgaga attctttccc ttatcatctg ctgccacact tgctccctga tgctctcaag
     3901 ggttgcctca tcaaaagatg ttgtgcctga gagtttgtaa atttcctgaa gcgactgaat
     3961 tctctgctcg taatcctgat acattatacg ttctcctgct acctgtccta tttcataata
     4021 ttttcgctgc cttatgcgtg cacttctgcc gccaccgaaa aagtcactta ttacaaagat
     4081 gaaaagcgaa agtccgataa gtattgcaac aaaaacaccc gctttctcac gtaaatattg
     4141 aatagctgcc atgtaaaata tatttaatta ttctgaagtt taatttaaac aggcgacaaa
     4201 tataacaaaa ttagattaaa tgattcatga ttgattactt cagaatatat aaacggagtt
     4261 tgtcgtctgc gttccatttc ttttcagtaa atcattattc agaatacata tctatcctta
     4321 ttttattctt gaattaatcc ggtcttgatt tctttaactc attttaattg tttttgtgaa
     4381 cgtgaaaaat aaagtatatt actttttcag aagagggatt ctatttttag agataattat
     4441 tgaaaaaaaa taaaaaaaga tgtattctct aagaaaaaaa tggggggtaa ttgtgcaaaa
     4501 aataaaaaaa ataaaaaaat atcttaaaat aataggagta ataaaaaata tattattaat
     4561 attgcgaaaa aaattattat ggcagaatta agattcaatg cattaaaaga gattttcaac
     4621 agaaaacctg ttgaaattga agattttccg tcaaatattt ctgagatttt tgctgtcaat
     4681 gtatttgatc ttcccaaaat ggagaggtac ctgcccaggg aggcttatct ggcagtaaag
     4741 aaatctatag atacgggtat gcccatttcg cgccagattg ctgatcaggt tgcaacaggg
     4801 ctcaaagcat gggctattga acgcggtgct actcattata ctcactggtt ccatcccctt
     4861 actgatgcta ctgctgagaa gcatgatgca ttccttgaaa ggacagacgg cagtaaaatg
     4921 attgagacat tttcaggcga gcttcttgta cagcaggagc ccgatgcatc aagctttccg
     4981 agcggaggta taagaaacac ttttgaggcg cgtggatata cagcatggga tgtctcttct
     5041 cccgcattta tagtgggcag tacactttgt attccaacaa tattcgtgtc atatacaggc
     5101 gaggcactcg attataaaac acccttgctt aaggcccttt cagctcttga taaggcagcg
     5161 gtgagagtct gtcagtattt cgataaggat gttaccaggg tctatgccac actcggctgg
     5221 gaacaggaat attttctgat tgacgaggca ctttattacg cacggcccga tctgatgctt
     5281 gccgaccgta ctcttatggg ccaccagtcg tcgaaagacc agcagctatc cgatcattac
     5341 ttcggttcaa tacctgaaag agttattgca tttatgaaag aatttgaact cgaagcctat
     5401 aaacttggaa taccggtgaa aacacgtcat aatgaagtgg cacccaacca gtacgaatgc
     5461 gctcctatat ttgaagaggt taatctggca gttgaccata accagcttct tatggatatt
     5521 atgagacgtg ttgctcgcag gcataacttc agagtcctct tccacgaaaa gccttttgca
     5581 ggcataaatg gctcaggaaa acataataac tggtcaatgg caacaaacac tggtgtaaac
     5641 ctcctttctc cgggcagaaa tccaaaaaca aacctgcagt ttcttacatt ccttgtaaat
     5701 gtaataaagg cagtaaacga taatcaggaa ttacttgctg caagtataat aaatgagcca
     5761 aattcacaca gactcggagg ccatgaggca ccaccggcaa ttatttctgt ctttattggt
     5821 aaatatcttt cttctgtact tgatgaaatt gtttcaaaag ttacaacaag aaagatgagt
     5881 ccttcgttaa aaacagacct gaaacttggc atcggaaaaa ttccggaaat acttctcgat
     5941 aatactgacc gcaaccgtac atcacctttt gcttttaccg gaaacaggtt tgagttcaga
     6001 gcagtaggcg cctcagcaaa ctgcggagct gccatgatag tgctcaacac tgctgttgcc
     6061 agacaacttg aaattttcag ctcggaagtt gatgctctta ttaaaaaggg aaggaaaaaa
     6121 gatgaagcta tattccaggt tcttaaaaaa tatattattg aatcacagag aataaggttt
     6181 gaaggcgaca attatagcga ggaatggaaa aaggaggctg ccagaagagg acttcatatc
     6241 attacaagtg tgcctgaaac gctgaaactt tatcttacgc cacagggtaa aagcctgctc
     6301 gttgaaacag gcattttcag tgaacgtgaa ctaaaaagca gagtggatgt ggaatatgag
     6361 aaattcatta aaaaagtcca gatccagtca cgggttcttg gagatcttgc tctgaaccac
     6421 attgtacctg tagcaattga ttatataaca tcactgctcg ataacgtaaa ggggttgaaa
     6481 gaagtttttc cggaagaaga atctgaaaat ctggctgctc caagaaaaga actgattaag
     6541 aaaatctctt atcacatttc agaaatccag agactggtac atgacatgat tgaggaaaga
     6601 aagaaggcga acgttatcga agatttatat gataaggcta tagcttatga aaggaacgta
     6661 aagccttacc ttgaagaaat acgcgagcac atcgacaagc tcgaaagaat agta
//
LOCUS       DAOSNB010000133         6709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11455_length_6709_cov_4.105651, whole genome shotgun sequence.
ACCESSION   DAOSNB010000133 DAOSNB010000000
VERSION     DAOSNB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11455_length_6709_cov_4.105651"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2907)
                     /locus_tag="PLG42_06480"
     CDS             complement(61..2907)
                     /locus_tag="PLG42_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOU30889.1"
                     /translation="MRQYIYFLLLLFTAGCVGGTGSQDEKISLAGEWNFLIDSLDVGV
                     NEEWYNKNLPDRVFLPGSMAENGKGYDVTVNTSWTGDIIDRSYFTDDKYARYREPGNI
                     KIPFWLKPVKYYSGVAWYQKEVNVPADWNNKKITLFLERCHWETTLFVNGRKAGTCNS
                     LATPHIYDLTGFLREGKNMLTVRVDNRIIIPIGVNSHSISDHTQGNWNGIAGEISLRV
                     SSPVSIDEVRIFPDIKGKKARVVVNIDNPAGSEFRGRLTMRAESMNSAAKKKLRKISK
                     DITAVMENAGFIFEYPMGNNPQLWSEFNPALYSLTVELKDKRGKTLDTRKEIFGMREI
                     KSSGTRLELNGKPIFLRGTTECCIFPLTGYPPSDTASWLRVMRILKEYGLNHMRFHSY
                     CPPEAAFIAADITGIYLHVECSSWANQGSSIGYGGPVDSFIYQEGDRIIKEYGNHPSF
                     CMLAYGNEPAGRNQNQFLGNLLKYWKSIDNRRVYTSAAGWPVIPENDYNLIPDPRIQH
                     WGEGLKSVINSLPPNTMFDFRDVVSKYNIPVVGHEIGQWCVYPDFKEIPDYTGILKPT
                     NFEIFKETLENNGMGNQAEDFLMASGKLQVLCYKADIEAALRTPGFAGFQMLQLHDFP
                     GQGTALVGILNAFFKSKGYVKPEEFRMFCNETVPLARMEKMIYRDGEQFKASIEIAHF
                     GREPIKQATIISCVKDKNSIIRSDSITIDELPVGNCIGVDTLSMELPGFTRAQKLTLE
                     VRIKNSDFVNRWDFWVYPSEIEPVAGDVHITEKIDDVTRSVLNNGGRVLLLTHGKVAH
                     GRGAEVAVGFSSIFWNTAWTRGQAPHTLGILCDPHHPVFNHFPTEYHSNYQWWDPVTH
                     SQAMKIDGFPETMKPLIQLIDTWFENRRLALAFEAKCGNGKLLVCSINLKDSLDSRPV
                     TKQLLFSMLEYMKSESFNPSESIELHKIEELIRK"
     gene            complement(2930..5383)
                     /locus_tag="PLG42_06485"
     CDS             complement(2930..5383)
                     /locus_tag="PLG42_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30890.1"
                     /translation="MKRISFFYLFFISGFLLVFQTEAQQVPRVVMAFNEGWRFYLGDT
                     ANAFMTGFDDSNWRMLDLPHDWSIEGRVDQKAPSGGAGGYFPTGIGWYRKHFIVRKNE
                     LGMNHVILFDGVYMNSDVWLNGKHLGHYPFGYNSFYYSLDPYLKEGENIIAVRVDNSL
                     QPNSRWYSGSGIYRKVWLIRRNYLHVRQWGVYITTPEVSDKEATVAVKTEIENAYALS
                     RKAIVSSVILNKNGIAEGTAETVVELAPGENTDIKQVISVESPELWSIETPVLYTLVT
                     RIIVNGKPVDEVRTPFGIREIIYDTDKGFFLNGKNVKMNGVCLHHDAGCLGAAVPVRV
                     WERRLKILKEMGCNAIRTSHNPVAPEFLDLCDRMGFLVMDEIFDEWKRGKVTYGYNKY
                     FDEWAERDLISFIRRDRNHPSVVMWSAGNEIREQPVEGGEKVLKWILDIFHSEDPTRP
                     VTVGCDNIAADDNPARVEFLNLLDIVGYNYVDRWHERRELFYSIDRHIHPEWKMVGTE
                     SVSVGGIRGDYSFGKDTSVLNPNYNTRMIRAEQLWKFVRMNSYVIGDFMWTGIDYLGE
                     SFWPSKNASSGVIDLCGFPKDGYYFYKSQWTTTPFIHIFPHWNWQGMEGKVIPVMAYT
                     NCDAVELIVNGRSYGEKRLEFPRQGASGRWNSYERPQVFPTTADLHLSWDVPYQPGKI
                     VAVGKKGGKIVCTEVIETTGPPAALRLTCDRNTINADNEDVVHVKVEAIDSQGRVVPT
                     ASNMIYYSVSGAGKLIGLENGNPVDHEPYKSNRRSLFNGLGLAIIQAGKEPGKIIIKA
                     SSDGMKDVSIEVNVVKPLK"
     gene            complement(5422..6687)
                     /locus_tag="PLG42_06490"
     CDS             complement(5422..6687)
                     /locus_tag="PLG42_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOU30891.1"
                     /translation="MSGVPSLQGWAMSCALTGCIAGSLLSGWSSDRFGRKRLLIVSAL
                     LFIVASAGTGSAKSFSEFIIFRILGGIGIGLASNLSPVYISEITPARYRGAFVSINQL
                     TIVIGILAAQIINWRIADPVPPGADINYILNSWNGQTGWRWMFYACGIPAFLFFTLMW
                     FVPESPRWLARNPDERIKVHRTFSKIGGVEYADAELNQVIETLNSDNSKSDLRELFKP
                     GVFKVLVIGVTLAVFQQWCGINVIFNYAQEIFAGAGYSVSDILFNIVITGSVNLVFTF
                     AGMFTVDRAGRKALMLLGSGGLAAIFAIVGMMYFIKLQGFPLLVMVVAAIACYAMSLA
                     PVTWVILSEIFPNRIRGTAMAIATFSLWSACFVLTYTFPLLNHLLKVSGTFWVYSFIC
                     VIGFWFILKKLPETKGKSLEQIEHELTGK"
BASE COUNT         1913 a         1601 c         1202 g         1993 t
ORIGIN      
        1 aaagggctga attacttcag cactttatat ttaaaggcct ttagtgcaca tacggaccta
       61 ttattttctg atcaattctt caatcttatg caattcaata ctttccgacg gattaaacga
      121 ttcacttttc atgtattcga gcatcgaaaa aagaagctgt tttgtaacag gtcggctatc
      181 aagactatcc ttcaaattta tgctgcaaac aagtaatttt ccatttccgc attttgcctc
      241 aaaagcaagg gccagcctcc tgttctcaaa ccatgtgtct attaactgaa ttagcggttt
      301 catcgtttca ggaaatccat caattttcat agcctgagaa tgtgttacag ggtcccacca
      361 ctggtaatta ctatgatatt ctgtcggaaa gtgattgaaa acaggatgat gcggatcaca
      421 taatatgccg agagtgtgag gtgcctgtcc tctggtccac gctgtattcc agaaaatgct
      481 ggaaaaaccg actgctactt cggcccctct tccatgagca actttcccgt gagtcagcaa
      541 aagaactctg cctccattat ttaatacact gcgggtaacg tcgtcaatct tttcagtaat
      601 atgaacatca cctgcaacag gctctatttc tgaaggatat acccagaaat cccacctgtt
      661 gacgaaatca ctatttttta tcctcacctc caatgtaagc ttctgagccc gggtaaatcc
      721 aggaagctcc atagataagg tatcaacgcc gatacaattg ccaacaggta attcatcaat
      781 ggtaatgctg tcactcctga taatagaatt cttatccttt acacagctaa taatagtggc
      841 ttgtttaata ggttctcttc cgaaatgagc tatttcaatg ctggctttaa attgctcgcc
      901 atctcggtaa atcatttttt ccattcttgc aagtggaaca gtctcattgc agaacattct
      961 gaactcttcg ggtttaacat aacctttaga tttgaaaaaa gcattcagaa ttcctacaag
     1021 agctgttccc tgtcccggaa aatcatgaag ttgaagcatt tgaaaaccag caaaacccgg
     1081 tgtacgaagt gcagcctcaa tatcagcctt gtaacacaat acctgcagct ttcctgaagc
     1141 cataagaaaa tcttcagcct gatttcccat accgttattt tcaagtgtct ccttgaaaat
     1201 ttcaaaattt gtcggcttaa gaatacctgt ataatcaggt atctctttaa aatcggggta
     1261 aacacaccat tgacctattt catgacctac aaccggaata ttgtattttg aaacaacatc
     1321 tctgaagtca aacatagtat taggcggcag agaattgata acgcttttca gcccttcgcc
     1381 ccagtgctga atcctgggat ccggtatcag attataatca ttctcgggaa tgacaggcca
     1441 gcctgctgct gatgtgtaaa cccttcggtt atcaatcgat ttccagtact taagcaggtt
     1501 gccaagaaat tgattctgat tcctgcccgc aggttcattt ccataagcga gcatgcaaaa
     1561 agagggatga ttgccgtatt ctttaatgat tctgtctcct tcctggtaaa tgaaactgtc
     1621 aaccggacct ccataaccta ttgaactacc ttgatttgcc catgaactgc attcaacatg
     1681 aagataaata cctgttatgt ctgctgctat gaatgctgcc tcgggcgggc aataagaatg
     1741 aaatctcatg tgattaagcc cgtattcttt aagtatcctc ataactctca gccaggatgc
     1801 tgtatcggaa ggtgggtagc ctgtcaaagg gaaaatacaa cattcggttg ttccccttaa
     1861 aaatattggc ttaccattta gttctaacct cgtacccgag gatttaattt ccctcattcc
     1921 gaaaatttct tttctggtgt caagagtctt gccacgctta tcttttaatt ctaccgtcaa
     1981 agaataaagt gcagggttga attcactcca cagctgcgga ttatttccca tcggatattc
     2041 aaaaataaat cctgcattct ccataactgc tgtgatatct tttgaaatct tccgaagttt
     2101 tttctttgcc gcactgttca tgctttctgc cctcattgtc agcctccccc tgaattctga
     2161 acctgccggg ttatcaatat tgaccactac tctggctttt ttccctttaa tgtccggaaa
     2221 tattcttacc tcatcaattg aaactggtga tgatacccgc agagatatct cacctgcaat
     2281 accgttccag ttaccctgag tatggtcgct tatactatga gaattgactc ctatcggaat
     2341 aataattctg ttatcaaccc tcacagtaag catgtttttc ccttctctga gaaaacctgt
     2401 caggtcgtaa atatgaggtg tagcaaggct gttacaggtg cctgctttcc ttccattaac
     2461 aaaaagagtt gtctcccagt ggcagcgttc gagaaatagt gtaatttttt tattattcca
     2521 gtcagcagga acattaactt ccttctgata ccatgcaact ccgctgtaat atttcactgg
     2581 tttaagccag aaaggaatct taatattgcc aggctcacga tatctggcat acttatcatc
     2641 tgtaaaatac gacctgtcaa taatatcgcc tgtccatgaa gtatttacag ttacatcata
     2701 acctttaccg ttttctgcca tcgacccagg taagaagacc ctgtcgggaa gattcttgtt
     2761 ataccattct tcattaacgc cgacatcgag cgaatcgatc aagaaattcc attccccggc
     2821 aagtgaaatc ttctcatcct gagaaccggt tccaccaaca caaccggccg taaataacaa
     2881 taataaaaaa taaatgtact gtctcattta aatttcttaa ttatgatttt tactttaaag
     2941 gcttaacaac attcacttca atacttacat ctttcatacc atcggatgaa gcttttataa
     3001 taattttacc cggttctttc cctgcctgga ttattgccag tcccagaccg ttaaagaggc
     3061 tcctcctgtt cgatttataa ggttcgtgat caacagggtt tccgttttcc aacccgataa
     3121 gttttcctgc tccgcttacg ctataataaa tcatatttga agctgtcggt acaacccgac
     3181 cctggctgtc aattgcttcc acctttacat gtacaacatc ctcattatca gcattaattg
     3241 tatttcggtc acatgtaagt cgcagtgccg caggtggtcc ggttgtttct atcacttcag
     3301 tacatacaat tttacctcct tttttcccaa ctgccacaat tttaccgggc tggtaaggaa
     3361 cgtcccacga aaggtggagg tcggcagtgg ttgggaatac ctgaggccgc tcataagagt
     3421 tccacctgcc tgaagcaccc tgacgtggaa attcaagtct cttttcacca tatgacctgc
     3481 cgtttacaat aagttcgaca gcatcacagt ttgtatatgc cataactgga atcacttttc
     3541 cttccatacc ctgccagttc cagtgaggga aaatatggat gaaaggtgtg gtagtccact
     3601 ggcttttgta gaaatagtaa ccatcttttg gaaacccgca caggtcaatc actccacttg
     3661 atgcattctt ggatggccag aaagattcac ccagataatc tatgcccgtc cacatgaagt
     3721 cgcctattac ataagagttc attctcacaa atttccatag ttgttcggca cggatcattc
     3781 ttgtgttgta attgggatta agtaccgacg tgtctttacc gaagctgtaa tcgcccctga
     3841 taccaccaac agaaacactt tcagttccaa ccattttcca ttccggatga atatgtctgt
     3901 cgatgctgta aaatagctcc ctcctttcgt gccatctgtc aacatagttg tatcctacaa
     3961 tgtccagaag attgagaaat tcaactcttg caggattgtc gtccgctgct atgttgtcgc
     4021 aacctactgt aactggtctg gtagggtctt ccgaatggaa aatgtcgagt atccatttta
     4081 gcactttttc gccaccttca acaggctgtt ccctgatttc attgcctgca ctccacatca
     4141 caaccgacgg atggttacgg tcacgcctga tgaatgaaat cagatctctt tcagcccatt
     4201 cgtcaaaata tttgttgtac ccgtaagtga ctttccccct tttccattca tcaaaaatct
     4261 catccataac aagaaagccc attctgtcgc aaaggtcaag aaactcaggt gccacagggt
     4321 tatgtgatgt tcttatggca ttacaaccca tctctttgag aatcttaagc cttcgctccc
     4381 acacccggac aggaacggct gcaccaaggc atccagcatc atgatgaagg cagacaccat
     4441 tcatctttac atttttccca ttaaggaaaa atcccttgtc agtatcataa attatttctc
     4501 tgattccaaa tggcgttctt acttcatcaa caggcttgcc gtttacaatt atccttgtga
     4561 ccagtgtata aagcactggc gtttcaatcg accaaagttc agggctctca acagaaatga
     4621 cctgttttat atctgtattt tctcccggtg caagttcaac tactgtttca gctgttcctt
     4681 cagcaatacc gtttttattt aatatgacag aagacacaat tgctttccgc gacaaagcgt
     4741 acgcattttc aatctcagtc ttaaccgcta ctgtggcttc tttatccgac acttcaggag
     4801 tagttatata tactccccac tgcctcacat gcagatagtt cctccttata agccacacct
     4861 tccggtatat acccgaccct gaataccacc ttgagtttgg ctggagagaa ttgtcgactc
     4921 ttactgcaat aatattttca ccttctttca aatacgggtc gaggctgtaa taaaagctat
     4981 tgtaaccaaa gggatagtgt ccaaggtgtt ttccgtttaa ccatacatcg ctattcatat
     5041 aaacgccatc aaagagaatg acatggttca ttccgagttc attctttctg acaataaaat
     5101 gttttctgta ccatcctatc cctgttggaa agtatcctcc tgcaccacca gaaggtgctt
     5161 tttgatccac ccttccttct atgctccagt catggggcag gtcgagcatc ctccagttac
     5221 tgtcgtcaaa gcctgtcatg aaggcatttg cagtatcacc gaggtaaaac ctccaaccct
     5281 cattgaaagc cattacaact cttggcacct gctgagcttc ggtttgaaat accagcagaa
     5341 aaccagaaat aaaaaagaga taaaagaaac ttatcctttt catattaaca tttttaagta
     5401 gatcactaat atcgtaaaag tctactttcc tgtcaattca tgttcgatct gttcaagtga
     5461 tttccctttc gtttcaggaa gctttttgag tatgaaccag aaacctatga cacatataaa
     5521 actatatacc cagaaagtgc ccgaaacttt aagtaaatga ttcagcaacg gaaaagtgta
     5581 ggtaagcaca aagcatgctg accacaagga gaaagttgct attgccatgg ctgttcccct
     5641 gatacgatta gggaatattt ctgataaaat tacccatgtt accggtgcca gcgacatggc
     5701 ataacatgca attgcagcaa ctaccattac aagtaaagga aacccctgga gtttaataaa
     5761 atacatcatt cccacaatgg caaaaattgc tgcaagtcct ccggagccta acagcatcag
     5821 ggcttttcga cctgccctat cgacagtaaa cattcccgcg aaagtaaata caagatttac
     5881 gcttcctgta ataactatat tgaatagaat atctgaaaca ctgtaaccag caccggcaaa
     5941 tatttcctgg gcataattaa agattacatt gattccgcac cattgctgga aaacggcgag
     6001 cgtaactccg ataaccagaa ccttaaatac gccaggttta aacaattcac gaaggtcaga
     6061 ttttgaattg tcagaattga gtgtctcaat cacctgattg agttcggcat cagcatattc
     6121 cacacctcct atttttgaaa aagtcctgtg aaccttaatc ctttcatccg ggtttcttgc
     6181 aagccatcgg gggctttcag gcacaaacca cataagggta aaaaacaaaa atgcaggtat
     6241 tccacatgca taaaacatcc agcgccagcc agtctgacca ttccatgaat tcagtatgta
     6301 atttatgtct gcacctgggg gcacagggtc ggctatcctc cagttgataa tttgtgctgc
     6361 aagaatacct attactattg taagctggtt gattgagaca aatgctccac ggtatctggc
     6421 aggtgttatt tctgaaatat agacaggtga aagatttgaa gcaagaccta tgccgattcc
     6481 tccaaggatt ctgaaaataa tgaactcact gaacgatttt gccgaacctg ttcctgctga
     6541 tgcaacaatg aacagtaatg cagagactat cagcaaccgt ttccttccaa acctgtcgct
     6601 gctccatcca gagagcaggg aaccagctat acatcctgtg agagcgcagc tcattgccca
     6661 cccctgaagg gaaggaactc ctgaaatacc atttaaattt taataaaac
//
LOCUS       DAOSNB010000134         6670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11558_length_6670_cov_5.014361, whole genome shotgun sequence.
ACCESSION   DAOSNB010000134 DAOSNB010000000
VERSION     DAOSNB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11558_length_6670_cov_5.014361"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /gene="fusA"
                     /locus_tag="PLG42_06495"
     CDS             <1..1966
                     /gene="fusA"
                     /locus_tag="PLG42_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU30892.1"
                     /translation="QMDWMVQEQERGITITSAATTTYWKVNNEEYKINIIDTPGHVDF
                     TVEVERSLRVLDGAVAIFCAVGGVEPQSETVWRQANKYHVPRIAFVNKMDRIGADYFG
                     VVHQIHTKLGAKPVPVQIPIGAEETFIGVVDLIEMKAYIWDDEASLGTKFRVDEIPEE
                     LVEEAEEWRMKMIESLAEVNDTILERYIEDHHSITEKELIEALRKCTISGIAVPVLCG
                     AAFKNKGIQKLLDAVVNFLPSPLDIGPVHGTDPKTNEKITREPDNDAPLAALAFKIAT
                     DPYMGRLVFMRIYSGKMRTGDMVLNVRTGKRERINKLFRMHANKQQPVEVMEAGDICA
                     GVGLKDLRTGDTLCAIHKPIALESMDFPEPVIGVAIEPKTQADYDRLSNALNKLAEED
                     PTFRVKIDPDSGQTIIQGMGELHLEILIDRLKREFQVECNQGVPQVAYKEAITTSCEY
                     RELFRKQTGGKGKYADILVELMPADDGFKGLQFVNEVSGGNIPREFIPAIEKGFREAM
                     MNGPLAGFPLESLKVILKDGSYHPVDSDSLSFEIAARQAFRHAAVKCNPVLLEPIMAA
                     EVVTPEEYVGDIISDFNRRRGRIEGMETRGGARVIKAKVPLAENFGYVTALRTITSGR
                     ATSTMEFSHYEEVPEEIAKRIVEITKGIIL"
     gene            1971..2276
                     /gene="rpsJ"
                     /locus_tag="PLG42_06500"
     CDS             1971..2276
                     /gene="rpsJ"
                     /locus_tag="PLG42_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOU30893.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKSTGAVVSGPIPLPTH
                     KRIFTVNRSPFVNKKSREQFQLSSYKRLLDIYSSTPKTIDALMKLELPSGVEVEIKV"
     gene            2298..2918
                     /gene="rplC"
                     /locus_tag="PLG42_06505"
     CDS             2298..2918
                     /gene="rplC"
                     /locus_tag="PLG42_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOU30894.1"
                     /translation="MQGLIGKKVGMTSLFDENGKNVPCTVLQAGPCVVTQIKTVEKDG
                     YSAVQLGFDDKKEKNTNKPELGHFKKAGVTPKRVLIEFTGFDTSEVKAGDVLTADIFK
                     TDNFVDVRGWSKGKGFQGVVKRHGFGGVGGQTHGQHNRGRAPGSLGASSFPSRVLPGM
                     RMAGRTGGKKVMAISLRIMKLIPEENILIVKGSVPGPKGGYVIITK"
     gene            2918..3547
                     /gene="rplD"
                     /locus_tag="PLG42_06510"
     CDS             2918..3547
                     /gene="rplD"
                     /locus_tag="PLG42_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOU30895.1"
                     /translation="MEVSVYNINGEETGKKVKLDDTIFGIEPNDHAIYLDTKQYLANN
                     RQGTAKAKERSEVSGSTRKIKRQKGTGTARFGDIKNPLFRGGGRIFGPRPRDYSFKLN
                     KKVKDLARKSALSYKAASNNIIVVEDFNFEVPKTKEMKKISDNLKLSDKKSLFVLPEQ
                     NKNVYLSSRNLPGVEVVTVSELTTYKIMNASTLVLAESAVEVLHGTFKN"
     gene            3563..3853
                     /gene="rplW"
                     /locus_tag="PLG42_06515"
     CDS             3563..3853
                     /gene="rplW"
                     /locus_tag="PLG42_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOU30896.1"
                     /translation="MSILLKPIVTEKMTGQGEKLNRYGFIVAKNANKIQIKKAVEELY
                     GVTVKSVNTMRCPGKEKIRYTRSGMVKGRTASVKKAIVTLAEGNTIDFYSNI"
     gene            3856..4680
                     /gene="rplB"
                     /locus_tag="PLG42_06520"
     CDS             3856..4680
                     /gene="rplB"
                     /locus_tag="PLG42_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOU30897.1"
                     /translation="MGVRKLKPVTPGQRHMLISAFDDITCAEPEKSLLVPLKKTGGRN
                     NDGKMTMRYIGGGHKRMYRLIDFKRDKDGIPATVKTIEYDPNRSARIALVCYADGEKR
                     YIIAPQGLKVGQQIMSGKNVAPEIGNTLFLSDLPLGAMIHNIELKPGKGAALARSAGT
                     YAQLIAREGNYATIKLPSGETRMVLASCRATVGIVSNPDHNLEKSGKAGRSRWLGRRP
                     RTRGVAMNPIDHPMGGGEGKASGGHPRSRKGIPAKGYKTRDKKKYSTKYIVERRKK"
     gene            4691..4954
                     /gene="rpsS"
                     /locus_tag="PLG42_06525"
     CDS             4691..4954
                     /gene="rpsS"
                     /locus_tag="PLG42_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOU30898.1"
                     /translation="MSRSLKKGPFIDYKLLKRVLEMNESGKKSVIKTWSRRSVISPDF
                     VGHTIAVHNGNKFIPIYITENMVGHKLGEFAPTRTFRGHSGNK"
     gene            4987..5427
                     /gene="rplV"
                     /locus_tag="PLG42_06530"
     CDS             4987..5427
                     /gene="rplV"
                     /locus_tag="PLG42_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOU30899.1"
                     /translation="MGARKRKRAEQIKEAKASQTRAILRNCPTSPRKMRLVADLIRGV
                     EVNKALDILKFSKKEASRRLEKLLLSAISNWQQKNEGAKVEESGLYVKEIYVDGGRTL
                     KRLRPAPQGRAYRIRKRSNHVTVILGSRLEEKPETAEVVENSSK"
     gene            5433..6131
                     /gene="rpsC"
                     /locus_tag="PLG42_06535"
     CDS             5433..6131
                     /gene="rpsC"
                     /locus_tag="PLG42_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOU30900.1"
                     /translation="MGQKVNPISNRLGLIRGWDSNWYGGKDFSGKLLEDTKIREYLNA
                     RLAKASISKIIIERTLKLITITVNTARPGIIIGKGGQEVDKLKEELKKITKKEVQINI
                     FEIKRPELDACIVANNIARQIEGKISYRRAIKMAIASTMRMGAEGIKVLISGRLGGAE
                     MARSETYKEGRIPLHTFRADIDYALAEAHTKVGRIGIKVWICNGEVYGKRDLSPNIGA
                     KTAKVKGLKRKGKK"
     gene            6167..6589
                     /gene="rplP"
                     /locus_tag="PLG42_06540"
     CDS             6167..6589
                     /gene="rplP"
                     /locus_tag="PLG42_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOU30901.1"
                     /translation="MLQPKKTKYRKQQKGRMKGNAQKGTQLAFGSFGIKALESAWITG
                     QQLEAARQAITRYMRREGQIWIRVFPDKPITKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LIEADGVPLEVAKEALRLGAHKLPIKTKFIIRRDYVTA"
BASE COUNT         2235 a         1340 c         1654 g         1441 t
ORIGIN      
        1 ccagatggac tggatggttc aggaacagga aagaggtata acaatcacat cagctgcaac
       61 aacaacatac tggaaagtta ataacgagga atataaaata aatataatag acacacctgg
      121 gcacgtcgac tttactgtgg aagtggagag gtcgctgcgc gtactcgacg gcgccgtggc
      181 tatattttgt gctgtcggag gtgttgaacc ccagtcggaa acagtatggc ggcaggcaaa
      241 taaatatcac gtccccagaa tagccttcgt aaataaaatg gacagaatcg gtgccgatta
      301 cttcggtgta gtgcaccaga tacacactaa actcggagcc aaaccggtgc cggtacagat
      361 acccattggc gcagaagaaa cattcatcgg tgtggtagat cttattgaaa tgaaggcata
      421 tatatgggac gatgaagctt ctctcggtac aaaattcaga gttgacgaga tacctgaaga
      481 acttgtcgaa gaggctgagg agtggagaat gaaaatgatc gaatcgcttg ccgaggtgaa
      541 tgataccatc ctggaaagat atatagaaga tcatcactca ataacagaga aggaacttat
      601 tgaagcattg aggaaatgca ctatttcggg tatagctgta cccgttctgt gcggagctgc
      661 tttcaaaaac aaaggcattc agaagctcct cgatgctgtg gtcaacttcc ttccatcacc
      721 tcttgatata ggtcctgtgc atggaacaga cccgaaaacc aatgagaaga taacgcgcga
      781 gcctgataat gatgctccgc tggctgcact tgcatttaag atagccaccg acccttatat
      841 ggggaggctg gtattcatgc ggatatattc aggaaagatg cgaactggag atatggttct
      901 gaatgttcgc acaggtaaga gggaacgtat caacaagctt ttccgcatgc atgcaaataa
      961 acagcagccg gtggaagtca tggaagccgg agatatatgt gcaggtgttg gtctgaaaga
     1021 ccttcgcaca ggcgatacgc tgtgtgccat ccataaaccc atcgcacttg aatcgatgga
     1081 tttccccgaa ccggttatag gtgttgctat tgaaccaaaa acgcaggccg attacgaccg
     1141 tctttcaaat gcattgaata aactcgccga agaagatcct acttttagag taaagattga
     1201 ccccgacagc gggcagacaa ttattcaggg aatgggcgag ctgcacctcg aaatactcat
     1261 cgacaggctt aagagggaat tccaggttga atgcaatcag ggtgttcctc aggtggctta
     1321 caaggaagct atcacaacat cgtgcgaata ccgtgaactc ttcagaaagc agacaggtgg
     1381 taaaggaaaa tatgccgaca tacttgttga acttatgcct gctgacgacg gttttaaagg
     1441 gctgcagttt gtaaatgagg tgagtggagg caatatacca agggagttta taccggcaat
     1501 agaaaagggt ttccgtgagg cgatgatgaa tggcccgctt gccggcttcc cgctcgaaag
     1561 tttgaaagtg atactcaaag atgggtcgta tcacccggtc gattctgatt cactttcctt
     1621 cgagatagct gcacgccagg ctttcagaca tgctgccgta aaatgtaacc ctgtgctgct
     1681 cgaaccaatc atggcagccg aagtcgttac acccgaagaa tatgtcggtg atatcataag
     1741 cgacttcaac agaagaagag gaaggataga aggcatggag acgagagggg gtgcacgcgt
     1801 aataaaggca aaagtgcctc ttgccgaaaa ttttggttat gtaacagcac tgagaacaat
     1861 aacatcagga agggccactt caacaatgga gttttcacac tacgaagaag tgcccgaaga
     1921 aattgcaaag agaatagtgg aaataacaaa aggaataatt ctataattat atgagccaga
     1981 aaattcgcat taaacttaaa tcttacgatc ataatctggt agacaaatct gccgaaaaga
     2041 tcgtaaagac tgtaaaatca acaggtgccg ttgtaagcgg acctataccg cttccaaccc
     2101 acaaaaggat ttttacagta aaccgctcac cctttgttaa caagaagtca agggagcagt
     2161 ttcagctttc gtcatataaa aggctgctag atatttacag ctcgactcca aaaacaattg
     2221 atgcactgat gaagcttgaa ctgcccagcg gtgttgaggt tgaaataaaa gtctgataac
     2281 agataaaaac tttaaatatg cagggattaa ttggaaaaaa ggtgggaatg acttcccttt
     2341 tcgacgaaaa cgggaaaaat gttccctgta cagtactgca ggccggacct tgcgttgtga
     2401 cccagataaa gactgtggag aaggatggct attcggcggt gcagctgggc ttcgacgaca
     2461 agaaggagaa aaacactaat aagcctgagc ttggtcattt caagaaggcc ggcgttactc
     2521 cgaaaagagt actgattgaa ttcaccggtt ttgacaccag cgaagttaag gccggagacg
     2581 ttctgacagc cgatatcttc aaaacagaca actttgtcga cgtaagaggc tggtcaaaag
     2641 gaaaagggtt tcagggtgta gtaaagcgcc atggtttcgg aggcgtaggc ggtcaaaccc
     2701 acggccagca caacaggggc agagcaccgg gttcgctggg agcttcttca ttcccttcaa
     2761 gagtcctgcc aggaatgaga atggcaggcc gtacaggcgg aaagaaagtt atggccataa
     2821 gccttagaat aatgaaactt atacctgaag agaatatatt aatagtgaaa ggatcagtac
     2881 ctggtccgaa aggtggttac gtaataatta caaagtaatg gaagtaagtg tatataatat
     2941 caacggtgaa gaaaccggga aaaaggtaaa actcgacgat accatattcg gcatcgagcc
     3001 taatgaccat gccatttacc tcgacaccaa gcagtatctt gcaaataaca ggcagggcac
     3061 tgcaaaagcc aaggaacgca gcgaggtatc gggcagtacc cggaagatca aacgtcagaa
     3121 gggaaccgga accgcaaggt tcggcgatat caagaaccct ctattcagag gcggcggaag
     3181 aatcttcggg ccaaggccaa gagattacag ctttaaactt aataaaaagg tcaaggacct
     3241 tgcacgtaaa tcggcccttt catataaagc cgcatctaac aatatcatcg tggtggagga
     3301 ctttaacttc gaagttccga aaacaaaaga gatgaagaaa ataagcgata atctcaagct
     3361 ttccgataaa aaatcactct ttgttttacc ggaacaaaat aaaaatgtat atttgtcctc
     3421 tcgtaatttg ccgggggttg aagttgtaac tgtcagtgaa ttaacgacat acaagataat
     3481 gaacgcttca actcttgtac ttgcagagag tgcagttgaa gttttgcacg ggacttttaa
     3541 aaactagtaa ctgaaaaaaa caatgagtat tctactgaaa ccgatagtaa cagagaaaat
     3601 gacaggccag ggggaaaagc tgaaccggta cggatttatc gtggcaaaaa atgccaacaa
     3661 gatacagata aagaaggccg tggaggaact ttatggcgtc actgttaaat cggttaatac
     3721 aatgcgatgc cctggcaaag aaaaaataag gtacacccgt tcggggatgg taaaaggcag
     3781 gactgcttca gttaagaaag caatagtaac tcttgccgaa ggtaatacta ttgattttta
     3841 tagcaatatt taaaaatggg agtaaggaaa ttaaaacctg ttacaccagg tcagagacac
     3901 atgcttatca gtgcatttga tgatatcaca tgcgctgagc cggagaaatc tctgcttgtg
     3961 ccgcttaaga aaacgggcgg aaggaacaac gatggcaaaa tgacgatgcg ctatataggc
     4021 ggcgggcata agagaatgta ccggcttatt gatttcaaaa gagataagga tggaattccg
     4081 gccacggtaa agaccattga atacgatcca aatcgttcgg cacgtatagc tctggtctgt
     4141 tatgctgatg gagaaaagag atatattatt gctcctcagg gacttaaggt agggcagcag
     4201 ataatgtcgg gcaaaaatgt tgcacccgaa ataggaaata ctctttttct cagcgacctg
     4261 ccactgggtg caatgataca caacattgaa cttaaacccg gcaagggagc agctctggca
     4321 cgcagtgccg gaacttatgc tcagctgata gcccgtgaag gaaactatgc aactataaaa
     4381 cttccttcgg gcgaaacccg tatggtactg gcatcatgcc gtgcaacagt tggaatcgtc
     4441 tccaaccccg accataacct tgaaaagtcg ggcaaggcag gccgctcgcg ctggctggga
     4501 aggaggccgc gtaccagagg tgtcgcaatg aacccgatcg accacccgat gggtggcggc
     4561 gaaggaaaag cctcgggagg ccacccgcgc tccagaaaag gtatccctgc aaaaggttat
     4621 aaaacaaggg ataagaaaaa atattcgaca aagtatatcg tagaaagaag aaagaaataa
     4681 taaggatatt atgagtaggt cactcaaaaa gggtccattt atcgattaca agctactgaa
     4741 gagagtgctt gaaatgaacg aatcaggtaa gaaatcggtt atcaaaacct ggtcgagaag
     4801 atcagtcatt tcacccgatt tcgtgggaca tactattgca gtccataacg gaaataaatt
     4861 cattccgatt tatattaccg aaaacatggt aggccataaa ctcggtgaat ttgctcctac
     4921 acgtacattc agagggcatt ccggaaataa atagtgaatg gtatagatta ttgatacagt
     4981 aaaaaaatgg gtgcgagaaa aagaaaaaga gccgaacaga taaaagaggc aaaagctagc
     5041 cagacaaggg ctatactcag aaattgccct acctcaccgc gcaaaatgcg ccttgttgca
     5101 gaccttattc gcggtgtgga agtcaataag gcacttgata ttctgaagtt cagtaagaaa
     5161 gaagcttcac gccgtctgga gaaactgctc ctttccgcca tatcaaactg gcagcagaaa
     5221 aacgagggag ccaaggtgga agagagcgga ctctatgtca aagaaattta tgttgacggc
     5281 ggacgtactc tcaaacgact cagaccggca ccacagggca gagcctacag aatcagaaaa
     5341 cgctccaacc atgtaacagt gatactgggc agccggctcg aggaaaaacc tgaaacagct
     5401 gaagtagtag aaaacagttc aaaataaaat aaatgggaca aaaagtaaat ccgataagta
     5461 accgacttgg tctcataaga ggctgggatt caaactggta tggaggtaag gacttctccg
     5521 gcaagctact tgaggataca aaaatcagag agtacctcaa tgcaagactt gccaaagcca
     5581 gtatctcaaa gattattatc gaaagaacac ttaagctcat aaccatcaca gtcaataccg
     5641 cacgcccggg tataattata ggtaaaggag ggcaggaggt tgataagctg aaggaagagc
     5701 tcaagaaaat aaccaaaaaa gaggttcaga taaatatctt cgagataaag agacccgaac
     5761 tggatgcctg tatagttgca aataatattg cccgtcagat tgagggcaag atatcgtatc
     5821 gcagagctat caagatggct atagcatcta ccatgagaat gggagccgaa ggcattaagg
     5881 tgctgatatc tggcaggctc gggggagccg aaatggcacg tagcgaaact tacaaggaag
     5941 gccgcattcc tctccatact ttcagagccg acatcgatta cgctcttgcc gaagcccata
     6001 caaaggttgg acgtatcggc atcaaagtat ggatatgtaa cggagaggtt tacggaaaaa
     6061 gagacctcag ccccaatatc ggagcaaaaa cagctaaggt aaagggactg aagagaaaag
     6121 gaaagaaata aatcaacgct gattaaaaat tttatagaat actgcaatgt tacagccaaa
     6181 gaagacaaaa tacaggaaac agcagaaagg cagaatgaag ggcaacgccc agaagggcac
     6241 tcagcttgct tttgggtcgt tcggaattaa agcgctggag tcggcatgga ttactggcca
     6301 acagcttgaa gctgcccgtc aggctattac acgctatatg aggcgtgaag gccagatatg
     6361 gataagagtc ttccccgaca aacccatcac aaagaaacct gctgaggtgc gaatgggtaa
     6421 gggtaaaggt gcacccgaag gatttgtggc acctgtaacc ccgggcagaa tacttatcga
     6481 agccgacggt gttcccctcg aagtggcaaa agaagctctg cgactgggag cacataagct
     6541 tccgataaag acaaaattta taatcagacg cgattacgta acagcttgat aaaaatatat
     6601 aaagatgaaa atgtcggaaa taagggaatt aagcacaccg gatattattg aaagaataga
     6661 taccgagaga
//
LOCUS       DAOSNB010000135         6654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11590_length_6654_cov_4.631005, whole genome shotgun sequence.
ACCESSION   DAOSNB010000135 DAOSNB010000000
VERSION     DAOSNB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11590_length_6654_cov_4.631005"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLG42_06545"
     CDS             complement(<1..893)
                     /locus_tag="PLG42_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30902.1"
                     /translation="MKKFGTLFLIVLAALAFTGCSGLNKMKKNASLVKYDVTPKVLET
                     HAGMVDVTIKGTYPQKYFDKKTILEITPVLVYQGGETAFKPVKVQGESVQANNEVISY
                     NGDSFTYNDKVQYKPEMKKSELYVRVKATRGSTTLDFDPVKIADGVIATSTLVDVHGK
                     PVFMPDKYQRIIPESVMADIHYIINRFDLRSSELKADDIKLLKEYISKVNQDPKRNFK
                     NVEISSYASPDGPFNFNEKLSGNRGSTADKFIRSEFAKIEAAKAAGFFNEKTTAEDWD
                     GFREELQKSNIKDKDLILRVLS"
     gene            complement(1018..1656)
                     /locus_tag="PLG42_06550"
     CDS             complement(1018..1656)
                     /locus_tag="PLG42_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOU30903.1"
                     /translation="MVKYNYIVIEGNIGAGKTTLATKIANDFNAKLILERFADNPFLP
                     KFYADPERYSFPLELSFLADRYKQLKEEFMKQDLFKSFTIADYYFAKSLIFAAATLTG
                     DEFNLYRQIFYIIYGMLPKPDLYVYLHLEPDRLIQNIQKRGREYEKLITTEYLKKIQD
                     NYFLFFRQNPENKYLIIDINSIDFVENEEDYSLIIDKIFNCEYTSGMNKIVL"
     gene            complement(1735..3945)
                     /locus_tag="PLG42_06555"
     CDS             complement(1735..3945)
                     /locus_tag="PLG42_06555"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOU30904.1"
                     /translation="MFNIFRKVIDLGDGRTITLESGKLARQSDGAVLLTMGKTMLLAT
                     VVAAKEVKEDTDFMPLSVEYREKYASSGRIPGGFLKREAKPSDNEILVARLVDRALRP
                     LFPDDFHAETFVNIYLVSADKEILPDALAGLAASAALTLSDIPFKGPISEVRVARING
                     QFVINPTASQVKEADMDLIVGATYENIIMVEGEMKEVSEAEMLEALKVAHEAIKVHCK
                     AQMEFAAEAGAPPKREYASEENEELRKKVWDETWDKCYQVAKSLIPVKQKRSEAFEAV
                     LNEFLSQFTEEDEVNEALVKKYYSEVHRDVARRLVLDEGIRLDGRKPDEIRPIYCEID
                     PLPATHGSALFTRGETQSLTTVTLGTKLDEKIIDEVLNQGTEKFTLHYNFPPFSTGEA
                     KPVRGISRREIGHGNLAHRALKNMLPPDEENPYTVRVVSDILESNGSSSMATVCAGSL
                     ALMDAGIKIRKPVSGIAMGLITDTKTGKYAILSDILGDEDHIGDMDFKVAGTRDGITA
                     TQMDIKVEGLSYEILAKALEQARIGRLHILDIMEQTIAEPRPELKPHAPRIEMVTIPR
                     DMIGALIGPGGKIIQEIQRETGTTIIVEEKNGVGEVEIFGENKEALLDAVERVKKIVA
                     VPEIGHVYKGIVKSVMQYGAFVEILPNKDGLLHISEIDWKKTNRVEDILKEGQEVEVK
                     LIDIDEKTGKLRLSRKALLPQPEGNEAEQTRPRSERDTQRRHPPRKNFRRRERE"
     gene            complement(4051..4320)
                     /gene="rpsO"
                     /locus_tag="PLG42_06560"
     CDS             complement(4051..4320)
                     /gene="rpsO"
                     /locus_tag="PLG42_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOU30905.1"
                     /translation="MYLTSEKKKEFFKTYGKSELDTGSTEGQIALFSYRINFLTEHLK
                     KNKKDFSTQRALIKLVGKRRKLLDYLREKDINRYREIIKALNIRK"
     gene            complement(4736..6061)
                     /locus_tag="PLG42_06565"
     CDS             complement(4736..6061)
                     /locus_tag="PLG42_06565"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOU30906.1"
                     /translation="MKEEFLHFIWKYGLYYKERLRTAEGEPVEVIHPGEYNRDAGPDF
                     FNARIKYRGIEWAGNVEIHTKASHFDMHGHNRDHAFDNVILHVVAENDRKVFNASGIE
                     ITTAEISFEEKMFERYISLVNNPCTIACQENITSIDRFRFRHWLGALVVERLVEKYEM
                     IRKMLDDTGNDWEEVFYRMISRYFGFKVNAEPFEMLATALPFRIIRKHSDNRLQVEAL
                     LFGAAGMLSEGLFKEALNDSYYKDLLREFKILKTKYSINPVHGWIWKFSKLRPANFPT
                     IRISQLAAMLSVTGGLFSRVAEAKDTGEIREVFNVSASEYWDTHYVFGRKTRYEKKNT
                     GDTATDILLINTVVPVLFTYGKVKDSNSFCLKAIDFLEEIAPEDNRIIKEWRDAGIEA
                     VSAFDTQGLLQLRNEYCCRRKCLKCRVGSELIARGIVLKNEDEIMLEPY"
     gene            complement(6265..>6654)
                     /locus_tag="PLG42_06570"
     CDS             complement(6265..>6654)
                     /locus_tag="PLG42_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOU30907.1"
                     /translation="LTSNKGSTDFQYHNEDGIKLFERVISVSQKWGTVDNIMYVVGAT
                     KPEMLKDVRKIAPEHFLLVPGVGAQGGSLQEVAKYGLNSTCGLLVNSSRAIIFADNSE
                     FFHRVAGDKAREMQVEMEKYLSQKKLI"
BASE COUNT         1743 a         1457 c         1199 g         2255 t
ORIGIN      
        1 gaaaggaccc tcaggataag atctttatcc ttgatgtttg atttctgaag ttcttccctg
       61 aagccatccc agtcttctgc agtggttttt tcattgaaga aacctgcagc ttttgcagct
      121 tcaatctttg caaattcact tctgatgaac ttgtcagctg ttgaacctct gtttccggaa
      181 agtttctcat tgaaattgaa cgggccatca ggtgaagcat aagagctgat ttcgacattt
      241 ttaaaatttc gtttcggatc ctgattgacc tttgatatat attctttaag aagtttgata
      301 tcatctgcct ttagctcaga gctgcgcagg tcgaaccggt taataatata atggatgtca
      361 gccattacac tttcaggtat tattctctga tatttgtcgg gcataaaaac tggtttcccg
      421 tgaacatcta ccagtgttga ggtggcaatc acaccgtcgg caattttaac cggatcaaaa
      481 tcgagtgttg tacttcctct tgtggctttc actcttacat agagttctga ttttttcatc
      541 tccggcttat actgcacctt atcgttgtat gtgaagctgt caccgttata tgatataacc
      601 tcattatttg cctggacact ttctccctga actttaacag gtttaaatgc agtttctcca
      661 ccctgatata caagtaccgg tgtaatttca agtatggtct tcttgtcgaa gtacttctgc
      721 ggataggtgc ctttaatagt tacatccacc atgccggcat gtgtttcaag cacttttgga
      781 gtaacatcat attttacaag gctggcattc tttttcattt tattcagtcc gctgcagcct
      841 gtgaatgcaa gtgcagcaag aacaattaaa aataaagttc cgaatttttt catagtcaga
      901 tagtttatgt ttttataacc aatattattt tctgttacaa tatattaaaa gttttatctt
      961 tctgcaaatc taataaaaaa acgttaataa caaagtattc tttatccgaa aattgcgcta
     1021 aagtacgatt ttgttcattc ctgaagtgta ttcacaatta aatattttat caataatcag
     1081 tgagtaatct tcctcatttt caacgaaatc aatactattt atatcgatga taagatattt
     1141 attttcagga ttttgcctga agaataaaaa ataattgtcc tgtatttttt tcaggtattc
     1201 tgttgtaatt aatttttcgt attcgcgtcc acgtttctga atgttctgaa tcagtctgtc
     1261 gggttcaagg tgaagataaa catacaagtc gggttttggt agcataccat atattatata
     1321 aaatatctgc ctgtacaggt tgaattcatc tcccgtcaga gttgcggcag caaaaatcag
     1381 agattttgca aaataatagt cggcaattgt aaatgacttg aacagatcct gcttcatgaa
     1441 ctcttccttg agttgtttgt agcggtcggc caggaatgag agttccagcg ggaatgaata
     1501 tctttcgggg tcggcataga actttggcag aaaaggattg tcggcaaacc tttcaagaat
     1561 gagtttagca ttgaaatcat ttgcaatttt tgtggcaagt gtggtttttc ctgcaccaat
     1621 attgccttcg ataacgatat aattatattt caccatcttc agaagaagca ggtagttttt
     1681 cgttcaaatc aaatttataa actgatgaat aaaatttcaa taaatggata gattttattc
     1741 tctttctctt cttctgaagt tcttccgtgg aggatgtctt ctttgcgtgt ctctctctga
     1801 tctggggcgg gtctgttcag cctcatttcc ttcaggctga ggcaggaggg cttttctcga
     1861 aagtcttaat tttccagtct tttcgtcaat gtcaatcagt tttacctcca cttcctgtcc
     1921 ttccttaagt atatcttcaa ccctgtttgt ctttttccag tcgatttccg aaatgtgtag
     1981 cagtccatct ttgttaggca gtatttcaac gaatgcacca tattgcatca ccgacttaac
     2041 tattccttta taaacgtgtc ctatttccgg aactgccaca attttcttta ctctttccac
     2101 tgcatcgagg agggcttctt tattctcgcc gaaaatctcc acctctccca ctccgttctt
     2161 ttcttccaca attattgttg tccctgtttc gcgttgaatc tcctggatta ttttgccacc
     2221 cgggccaatg agggcgccaa tcatatcgcg cggtattgtc accatctcta ttctcggagc
     2281 atggggcttc agttcgggac gcggctcagc tattgtctgc tccataatgt caagtatgtg
     2341 cagccttcct attcttgcct gctcaagggc ttttgcaagt atttcatacg aaagtccctc
     2401 cacctttata tccatctgcg tggctgtaat accgtcgcgt gtgcctgcta ctttgaaatc
     2461 catgtcacct atatggtctt catcacccag aatatccgaa aggatggcat attttcctgt
     2521 tttggtatcg gtgattaatc ccatggcaat acctgatact ggttttctga tttttatacc
     2581 tgcatccatc agtgccagtg agcctgcaca tactgtagcc attgaagatg agccattcga
     2641 ctcaagtata tccgaaacaa cccttacagt gtagggattt tcctcatcag gtggaagcat
     2701 atttttgagg gctctgtgtg ccaggttgcc atgtccgatt tccctgcggc tgatgcccct
     2761 tacgggtttt gcctcacctg tggagaaagg cgggaaattg tagtgaagtg tgaatttttc
     2821 cgtaccctga ttaagcactt catcaataat tttttcatca agcttagttc ccagggtgac
     2881 agttgtaagt gactgcgtct cgcctcttgt aaaaagagca gaaccatgtg tggcagggag
     2941 ggggtctatc tcgcagtaga taggccttat ctcatcgggt tttcttccgt caagccttat
     3001 accttcatca agaacaagcc tgcgggcaac atccctgtgt acttcgctat aatatttctt
     3061 tacaagagct tcattaacct catcttcttc agtaaactgg gaaagaaact cattcagcac
     3121 agcttcaaat gcttccgacc ttttttgttt tacgggaata agactttttg ccacctgata
     3181 acatttatcc catgtttcat cccaaacttt tttcctcagt tcttcatttt cttcactggc
     3241 atattctctc ttcggcgggg ctccagcttc agcagcaaat tccatctgag ccttgcagtg
     3301 caccttgatg gcctcatgtg ccaccttaag ggcttcaagc atctcggctt cagaaacctc
     3361 cttcatctca ccctcaacca ttatgatatt ttcgtaagtg gctccaacaa tcaggtccat
     3421 gtctgcctct ttaacctgtg aagcagtagg gttaattaca aactgcccat ttattcttgc
     3481 aactcttact tctgaaatag gccctttgaa agggatatcc gaaagggtga gtgcagctga
     3541 agctgccaga cctgcaagtg catcaggcaa tatctcctta tcggctgaaa ccagataaat
     3601 atttacaaag gtctctgcat gaaaatcgtc agggaataaa ggccgtaaag ctctgtcaac
     3661 caggcgtgca accagtatct cattatccga aggcttggct tctctcttca gaaaacctcc
     3721 cggtatccgg cccgacgatg catatttttc cctgtattct accgatagag gcataaaatc
     3781 agtatcctct ttcacctcct ttgcagcaac aacagttgcc agaagcattg ttttacccat
     3841 tgttagcaat actgctccgt cggactgcct tgccagcttg ccgctctcaa gtgttattgt
     3901 acgtccgtca ccaagatcta tgactttcct gaaaatgtta aacatgatac tgatttaata
     3961 ctgttttaat actatttaat actgatttaa taagaagcga atttactaaa taaaaagcat
     4021 aaaaaaaggc aatttgacat tgcctttttc ctatttgcgg atattaagcg ctttgatgat
     4081 ttcgcggtac cggtttatat ctttctcacg aaggtaatcg agaagtttcc tgcgtttacc
     4141 tacaagctta ataagagccc gctgggtact gaagtctttc ttattcttct tcagatgctc
     4201 ggtgagaaaa ttaatacggt atgaaaaaag tgcaatctgc ccttccgttg aaccggtatc
     4261 cagttccgac ttcccgtaag tcttaaaaaa ttcctttttc ttctctgatg tcaaatacat
     4321 gttctttaca aaaatattgt taatccaaat gtaaatcgta tcacttaatt tggaacgcaa
     4381 aaataagact tttttattta atctctcaag taatcgcact tttttttaat attttttata
     4441 aatttttctt tttcagcaat ttttacagat ataatttttt aatccttttt taataatgac
     4501 gcaaaaacca tgccccgggt tgcgtataaa cgcagaaata aatcaactgt ttgtcaaata
     4561 ttttgcattg tttatcaaaa tgattctaac caaaacattt tcctttaatt ttataagtct
     4621 ttagaaatcc ggattgattt tatcaatcag aatactctga taagtttcat atttttttat
     4681 catcctgcta atacaccctc taaatcttta tcatactctt aaatttactt gctgtttaat
     4741 aaggttcgag cattatctca tcctcatttt taagaacaat tccgcgggca ataagttcac
     4801 tgcccaccct gcatttaagg cattttcttc tgcagcagta ttcattcctc agctgtagaa
     4861 gtccctgggt atcaaaagct gaaacagctt caatacctgc atctctccat tctttaataa
     4921 tcctgttatc ttccggtgct atctcttcaa ggaaatctat tgctttcaag cagaaactgt
     4981 tgctgtcctt cacctttcca taggtaaaaa gaacaggtac cacagtattt atcagtagta
     5041 tgtcggtcgc cgtatcacct gtattctttt tctcatacct tgtttttcgt ccgaaaacat
     5101 aatgagtgtc ccagtactcc gatgctgata cattgaaaac ttctctaatt tctccggtgt
     5161 cttttgcttc tgcaactctt gaaaatagcc cgcctgttac tgaaagcatg gcagcaagtt
     5221 gtgaaatcct tatcgtggga aaatttgcag gcctcagttt actgaacttc cagatccagc
     5281 cgtgtacagg attaatcgaa tattttgttt ttaaaatctt gaattctcta aggaggtctt
     5341 tataatagct gtcgttcaat gcctctttga agagaccttc gctgagcatt cctgctgcgc
     5401 cgaaaagaag agcctctacc tgcagcctgt tgtcgctatg ttttcttata attctgaaag
     5461 gtagagccgt agcgagcatc tcaaagggtt ctgcgttgac tttaaaaccg aaataacgtg
     5521 atatcatcct gtaaaaaact tcttcccaat catttccggt atcatcgagc atttttctga
     5581 tcatttcata cttttcaacc agcctttcta ccaccagagc tccaagccag tgtctgaagc
     5641 ggaacctgtc gatgctcgtg atattttcct gacaggcaat ggtgcagggg ttgttaacca
     5701 gactgatata cctttcaaac attttctcct caaaggagat ttctgctgtt gttatctcaa
     5761 tgcctgatgc gttgaacact tttcggtcgt tttctgctac cacatggaga ataacattgt
     5821 cgaaagcatg atctctgtta tggccatgca tgtcgaaatg cgaagctttc gtgtgtatct
     5881 ccacattacc ggcccattca atgcccctgt attttattct tgcattgaaa aagtctggac
     5941 cagcatcacg gttgtattcc ccggggtgga tgacctctac aggttctcct tcggcggttc
     6001 tcagcctttc tttgtaataa agaccatatt tccagatgaa atgcagaaat tcctctttca
     6061 ttttagcacg gtttaaatgc agcgaagtaa atatatgaaa aattttttct aaggaagata
     6121 aaataatacc actctgatta cacagttgca acagattttc acggattttt taatggttat
     6181 ataataatat ccctgtgtga aaataccagc ccgggatgaa tatcaaccgt gtgctataat
     6241 aaattttccg gttattatta aaattcagat aagttttttc tggctgagat atttttccat
     6301 ctcaacctgc atttctcttg ctttatctcc tgccacacgg tggaagaatt cagagttgtc
     6361 ggcgaaaatg attgctcttg atgaattaac cagcaagcca catgtgctgt ttagaccgta
     6421 ttttgcaact tcctggaggc tgccgccctg ggctccaaca cccggtacaa gaagaaaatg
     6481 ctcaggtgca atcttcctga catctttaag catttcgggt tttgtggcac caaccacata
     6541 cattatgttg tctaccgtac cccacttctg tgaaactgaa attacccttt caaaaagttt
     6601 tattccgtct tcgttatgat actggaaatc ggtagaacct ttgttggatg tgag
//
LOCUS       DAOSNB010000136         6647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11615_length_6647_cov_4.104066, whole genome shotgun sequence.
ACCESSION   DAOSNB010000136 DAOSNB010000000
VERSION     DAOSNB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11615_length_6647_cov_4.104066"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="PLG42_06575"
     CDS             <1..1694
                     /locus_tag="PLG42_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU30908.1"
                     /translation="FTPEGRQRLVVEDFINEFGNRAYKINGKYIQIRGGGWTDDLLLR
                     EDTAKLEAQVRYTRLMNLNTIRLEGFWGSSHALYDLCDRYGILLMAGWSCQWEWQEYI
                     GGKPCDEFGAVKSPEDIDLVSRMMHDQVTMFRNHPSIFVWVFGSDKLPRPELEKRYID
                     DLKNTDPTRPTLSACSRRTSEVSGPTGVKMEGPYDYVTPNYWYVDSLHGGAYGFNTET
                     GPGPQIPPIETLQKMFPQDKIWPVNDYWDYHCGRNEFNTINRYRHAMDMRYGSSRNLE
                     EFLKKAQLMNYEAIRPMFEAFAVNKAKATGIIQWMLNAAWPKLYWQLYDYYLMPNGAF
                     FGTGKACEPLHAVYNYGDHCIYLSNDLYQPRENLKLTIQAIDMEGNFLVNDSVTTEIQ
                     EYSSKKVYDMPAFNPDTKVYFLNLRLTENANSLISENFYWLSSKEDVLDEKKSEWFVT
                     PNSSYADFTALNHMPSATLEVTASKSENDDLSTINITLKNPSSVVAFFVEMKLKNHAN
                     GDLVLPVYWSDNYVSLLPGKSKNITVQFNNSSLKGGKPIIEVSGWNIPKFIIESL"
     gene            complement(2118..2297)
                     /locus_tag="PLG42_06580"
     CDS             complement(2118..2297)
                     /locus_tag="PLG42_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30909.1"
                     /translation="MKKDWFILELIRSLPFKILVFLVLFIFSGVYISKCKERQKKVQM
                     KEDVLYIHPKPKVQK"
     gene            complement(2480..3028)
                     /gene="rsmD"
                     /locus_tag="PLG42_06585"
     CDS             complement(2480..3028)
                     /gene="rsmD"
                     /locus_tag="PLG42_06585"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOU30910.1"
                     /translation="MRIISGKYKGRHIELPDNIRARPTTDFAREGLFDILSNRIDFEK
                     VDVLDLFSGTGSISFEFVSRGTKMVHLVEYDRYNISFIRQNIRALKIENIKPLRLDVR
                     DFLKICKHKYNIIFADPPYDLPWLRDIPDMVFESNILKVDGFLIIEHPGNIKFNDHPF
                     FYEHRKYGRVNFTFFRKPPILE"
     gene            complement(3019..3876)
                     /locus_tag="PLG42_06590"
     CDS             complement(3019..3876)
                     /locus_tag="PLG42_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOU30911.1"
                     /translation="MIKKVMPLLEVFDETLDINSTGNYILSLQASHEELSYCIADTFR
                     NRFILLRSYTPEENNRFNHEQISEIIFGDDFLERKYNKKFLVMPSAEFTLVPSPLYDP
                     AKRDEYFYFNHGEQENRVIIANKLPENDSYLIFSVSKPLYDTTISFFPDVFPYHHLRP
                     LFNQMVHAMKSQTGHYIHLHIENDFFNLAIFRELKLQYLNAFTYRNISDIMYFALNVF
                     KTLGIQGDTTVNLSGVTERYDDLNSTMSLYIKNINFVVPAGNFSFSHVFNDIPLHRFI
                     NLFSIVSCE"
     gene            4018..5436
                     /locus_tag="PLG42_06595"
     CDS             4018..5436
                     /locus_tag="PLG42_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU30912.1"
                     /translation="MRENLIYNHLLKHLNNIPTDDQSAAMKKIASFLCDSNQDIIFLL
                     TGYAGTGKTTLISSIVKTLELLRMRTILLAPTGRAAKVLSSITGCQAFTIHKKIYRQK
                     SSKDGIGQFVLDRNLTKDTLFIVDEASMVSNNPSELSIFGSGRLLEDLIEYVYSGVNC
                     RLMLVGDTAQLPPVGSEISPALDPEQLKEFGFNVITAELRQVLRQNEKSGILMNATKI
                     REQIESGDLSRPAIDCVNFKDIVRLSGNDLIDEINSAYDKCGLDGTIIVVNSNKLANK
                     YNQGIRSRIFFREEEISPGDIIMVVKNNYSLADEEDPYRFIANGDIAEVLRVSKYEER
                     YGFRFALMSLRFPDYSMEVDAKVMLDVLHLDSPALPAEKSSELFNAILQDYHNIKNKR
                     KQYEAIREDPYFNALQIKFAYAVTCHKAQGGQWERVFIDQGLFNKTEITLDYLRWLYT
                     ALTRATGKLYLVNFSEDFFLPC"
     gene            complement(5471..5887)
                     /locus_tag="PLG42_06600"
     CDS             complement(5471..5887)
                     /locus_tag="PLG42_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU30913.1"
                     /translation="MKSYRKELWFNTPTRRAFINITGEVEKCLAESGIKEGLCLVNAM
                     HITASVFINDDESGLHHDFEVWLEKLAPEKPYSQYRHNGYEDNADAHLKRQVMGREVV
                     VAVTNGRLDFGPWEQIFYGEFDGKRSKRVLVKIIGE"
     gene            complement(5891..>6647)
                     /locus_tag="PLG42_06605"
     CDS             complement(5891..>6647)
                     /locus_tag="PLG42_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOU30914.1"
                     /translation="TAALLMYYTLLQYKEKGLFKGNEYVIKTIVTSDLLDRIAESFNV
                     ECYNVLTGFKYFAELIRNLEGKKKYIGGGEESYGYLPGDYVRDKDAVGSCALIAETAA
                     WAKEHGMTMYELLIDIYLKYGLYLERLINLVRKGKEGAEEIKEMMHMYRNNPPEKINN
                     SRVVRIDDYEKLVSYDKLTGKTSKLKLIRSDVLQFFLADGSKISVRPSGTEPKIKFYF
                     SVNTKLDSATEFNDKLKLLNERIDGIISDLKLL"
BASE COUNT         2076 a         1306 c         1251 g         2014 t
ORIGIN      
        1 aatttactcc cgaaggacga caacgtttag tcgttgaaga ttttattaat gaattcggca
       61 accgagccta taaaataaac ggtaaatata tacaaatcag gggtggaggt tggactgacg
      121 atttattgct acgcgaagac acagcaaaac tggaggcgca ggttcgttat acccggttga
      181 tgaatctaaa taccattcgt cttgaaggtt tctggggaag cagccatgct ctatacgacc
      241 tatgcgaccg gtacggtata ctgctcatgg ccggatggag ctgccagtgg gaatggcaag
      301 agtatattgg aggaaaacca tgtgacgaat ttggcgctgt taaatcgccc gaagatattg
      361 accttgtttc gcgcatgatg catgaccagg taactatgtt cagaaaccac ccgagtattt
      421 ttgtatgggt gtttggaagc gataaacttc cacggcctga gcttgaaaaa aggtatattg
      481 acgatttgaa aaacacggat cctacaagac caaccctgag tgcctgctcg agacgtacca
      541 gcgaagtctc gggaccaaca ggagtaaaaa tggaaggacc ctatgactat gtcacaccca
      601 actactggta tgtggattcc cttcatggag gtgcctacgg attcaatacc gaaacaggtc
      661 ccgggcctca gattccaccg attgaaacac tgcagaaaat gtttccacag gataaaattt
      721 ggcctgtaaa tgattactgg gattaccatt gcggaagaaa cgaatttaat accataaacc
      781 gttatcgtca tgcaatggat atgcgttatg gttcctcccg taatctggaa gaattcttga
      841 agaaagccca acttatgaat tatgaagcca ttaggccaat gtttgaagct tttgcggtta
      901 acaaggcaaa ggctactgga attatccagt ggatgctcaa tgccgcatgg ccaaaacttt
      961 actggcagct ttacgattac tatctcatgc ctaatggtgc attttttggt accggaaaag
     1021 cttgtgaacc cctgcatgct gtttataatt acggagatca ttgcatttac ctttcgaacg
     1081 acctgtatca accaagggaa aatcttaaac tcacaattca ggctattgac atggaaggca
     1141 acttcctcgt aaatgattca gttactactg aaatacagga atatagctca aagaaagtgt
     1201 acgatatgcc ggcttttaat ccagacacaa aagtttattt tctgaatctc aggcttactg
     1261 aaaatgccaa ttcacttatc agcgaaaact tttactggtt atcttcaaaa gaagatgttc
     1321 tggatgagaa aaaatcagaa tggtttgtta cacctaattc atcatatgct gattttacag
     1381 ctctgaacca catgccgtct gccacccttg aagtaacggc cagtaaatca gaaaatgatg
     1441 acctgtctac aattaatata accctgaaaa atccttcttc ggttgtggca ttttttgttg
     1501 agatgaagtt aaaaaaccat gctaacggcg atctggtgct tcctgtttat tggtccgata
     1561 attatgtatc cctactcccg ggcaaaagta aaaatataac agttcagttt aacaacagct
     1621 cactaaaagg aggtaaaccc attattgagg tgtcaggctg gaatattcca aaatttatta
     1681 ttgaatcact ttagttaaga aaagaaacat atttattgtt attctggtac tggtcatttt
     1741 tttacaattt catttctgac caatattctt ggcctataat acaagcatag atttatatta
     1801 gtaaaactga ccgacatttt tggccttcga accgaaatgc tgttccttta cctaccctcg
     1861 gatatttttc agaatcggaa tcttgactaa accatttgta aataatgcaa cgattgattt
     1921 aaacccgaaa agcctttttt atcctcgaaa aaaaactaaa cagtatatta tgcaggttgt
     1981 ttcgcattga aactatttta aaggtcaact tattacatga atagaacatc aggaaatttg
     2041 ttatttaaaa tgaaagaaaa tgttacgtaa gcaaactatt ggtagtatcg taaaaaccag
     2101 agctatgacc ggaaaactta tttctgaact ttgggcttag gatgaatgta caaaacatcc
     2161 tccttcatct gaaccttctt ctgcctttcc ttgcactttg aaatgtaaac tccagaaaaa
     2221 ataaacaata ccagaaatac cagaatttta aatggcagac tcctgattaa ttcaagtatg
     2281 aaccagtctt ttttcatatc cagcctgaaa ctgatttata caaagaccca ataactttac
     2341 caaaagttgc ctctgacaac taacatcctg aagctctgac cctgagaagg catatcaaaa
     2401 ttaataaatt aaaattaaaa taaaaaacct ggagttattt ttagtcagta agttaattgt
     2461 tccaggaatt caaagtatgt tattccagta ttggtggttt tcggaaaaag gtaaaattaa
     2521 ctcttccata ttttctatgc tcgtaaaaaa aaggatgatc attgaattta atattgcctg
     2581 gatgctcaat tatcagaaaa ccatctactt tcagaatatt gctttcgaac accatgtcgg
     2641 gaatatccct gagccacggt aagtcatatg gcgggtctgc aaatattatg ttgtatttgt
     2701 gcttacaaat ctttaaaaaa tccctgacat caagcctcaa aggctttatg ttttcaattt
     2761 taagagctct aatattctgt ctgataaaag atatgttata acggtcatat tcaaccagat
     2821 gaaccatctt tgttcccctc gagacgaact caaagcttat gcttcctgta cccgagaaaa
     2881 gatcaagtac atcaactttt tcaaaatcaa tcctgttcga aagaatatca aacaagcctt
     2941 cacgggcaaa gtctgtggtg ggccttgccc ttatattatc aggtaactca atatgcctgc
     3001 ctttatattt tccgcttatt attcgcaact tactatactg aataaattaa taaatctgtg
     3061 caagggtata tcattaaaga catgactgaa tgaaaaattt cctgctggca caacgaaatt
     3121 tatatttttg atatacaacg acatagtcga attaagatca tcatatcttt ctgttacacc
     3181 gcttagattt acagttgtgt caccttgaat tccaagtgtt ttaaacacat tcagggcaaa
     3241 atacataata tccgaaatgt tcctgtaagt gaatgcatta agatattgca gttttaattc
     3301 gcggaatatt gcaagattga aaaaatcatt ttcaatatga agatgaatat agtgacctgt
     3361 ctgagatttc atggcatgga ccatctggtt gaaaagtggg cgaagatggt gataaggaaa
     3421 aacatctggg aagaagctta tagttgtatc atataatggc tttgaaacac tgaatatcag
     3481 atatgagtca ttttcaggta gtttgtttgc aataattacc ctgttctcct gttctccatg
     3541 attgaaatag aaatattcat ctctttttgc cggatcatac aatggcgagg gtacaagagt
     3601 gaactcggca gaaggcatta ccagaaactt tttattgtac tttctttcaa gaaaatcatc
     3661 cccaaaaata atttctgaaa tttgttcatg gttaaacctg ttattttctt caggagtata
     3721 tgaccttagt aaaataaacc tgtttctgaa agtgtctgca atacaatatg atagctcttc
     3781 atggcttgcc tggagcgaca gtatataatt gcctgtagag tttatgtcga gagtctcatc
     3841 aaatacttca agtaacggca taactttctt tatcaatact ttgtgttttc catgtgccct
     3901 ttgtgttctc tgtggccaaa cttcaaaaag ccggtggcct tgttaactac aagaggttat
     3961 acaaatataa attaaagaat gattatgttt acaaaacaat aaattatcct gcagctgatg
     4021 cgtgagaatc tgatatacaa tcatttatta aagcatctca ataatattcc tactgacgac
     4081 cagtcagcgg cgatgaaaaa gattgcctct tttctctgcg acagcaacca ggatatcata
     4141 tttctattga caggttatgc aggaactgga aagacgacac tcataagttc aattgtaaaa
     4201 accctggaac ttctgaggat gaggacaata ctgctggctc ctaccggcag ggcagcaaag
     4261 gtgctctcat caataactgg ttgtcaggca ttcactatcc ataagaaaat ataccgacag
     4321 aaatcatcaa aagatggtat cgggcaattt gtccttgaca gaaaccttac aaaagacaca
     4381 ctttttattg tcgatgaggc atcgatggta tcgaacaatc catcagagct atcgatattt
     4441 gggagcggaa ggctgctgga ggaccttata gaatatgtgt actcaggcgt caattgcagg
     4501 ttaatgcttg tgggagacac tgcacagctg cctcctgtgg gctcggaaat aagccctgca
     4561 ctcgatcctg aacaattaaa ggaatttggt tttaatgtta taaccgcaga acttcgccag
     4621 gtactaagac agaatgaaaa atctggcata ctgatgaatg ctacaaaaat aagagaacaa
     4681 atagaatcgg gtgatctctc cagacctgca attgattgtg tcaattttaa agatatagtg
     4741 agactttcgg gaaatgacct cattgatgaa atcaactctg catacgataa atgcggcctg
     4801 gatgggacaa taattgttgt aaactcaaat aagctggcaa acaaatacaa ccagggaata
     4861 agaagcagaa ttttcttcag ggaggaggaa attagcccgg gagatataat tatggtagtg
     4921 aaaaacaact attctcttgc tgatgaagaa gatccttaca ggtttattgc caatggagat
     4981 attgctgaag tactgagagt aagcaaatat gaggaaaggt atggattcag attcgctttg
     5041 atgtcgttaa gatttcccga ttacagcatg gaagtggatg caaaagtcat gctcgatgtt
     5101 cttcatctcg attctcctgc attgcctgcc gaaaagagca gcgagctgtt taatgccatt
     5161 ctccaggatt atcataatat taaaaacaag cgaaagcagt atgaggcaat ccgcgaagat
     5221 ccatatttta atgcattgca gattaaattc gcatatgctg taacatgcca caaagctcag
     5281 ggaggccagt gggagagggt cttcattgat cagggtttat ttaacaagac tgagataaca
     5341 ttagattacc ttcgctggct ctatactgca cttacaagag ccaccggtaa actctatctg
     5401 gtaaatttca gcgaagattt cttcttgccc tgctgaattt atttaatttc aacaaattgt
     5461 aagatgatgt ttattcccct atgattttaa ccagtaccct tttgctccgt ttcccgtcaa
     5521 attctccata gaatatctgc tcccatggcc cgaaatctaa cctgccattg gttactgcta
     5581 caactacctc acgacccata acctgacgtt taaggtgagc atcggcattg tcttcataac
     5641 cattatgcct atactggctg tatggctttt ccggagccag tttttcaagc catacctcga
     5701 aatcatgatg caatccgctc tcatcatcgt tgatgaaaac gcttgccgta atatgcattg
     5761 catttaccag gcacaatcct tccttaattc cgctttcggc aagacatttt tccacttccc
     5821 ccgtaatatt gataaaagcc ctgcgggtag gggtgttaaa ccataattcc tttctgtaag
     5881 acttcatatt ctataaaagt ttcaaatcag aaataattcc atcaattctt tcgttcagaa
     5941 gttttagctt atcattgaat tccgtggctg agtccagttt tgtgttcact gagaaataga
     6001 atttgatttt tggctctgtt cctgaaggcc tcacggagat ttttgaaccg tcggcaagga
     6061 aaaactggag cacatctgac ctgataagtt ttagtttgct ggttttacca gtcagtttgt
     6121 cgtatgaaac aagtttctca taatcatcta ttctgaccac acggctgttg ttaattttct
     6181 caggaggatt gtttctgtac atgtgcatca tttcctttat ctcttcagca ccttccttgc
     6241 ctttcctcac aagattgatt agcctttcaa gatagagtcc gtattttaaa taaatatcga
     6301 taagcaattc gtacattgtc attccatgct ctttagccca tgctgctgtt tcggcgataa
     6361 gtgcacatga accaaccgca tctttgtcgc ggacataatc accaggaagg tatccatagc
     6421 tctcttcccc acctccgata tatttctttt tgccttccag atttctgata agttctgcaa
     6481 aatatttaaa tccagtgaga acattgtagc actctacatt aaaagattct gcaattctgt
     6541 cgagcagatc actggtaaca atagttttga ttacgtattc atttcctttg aaaagtcctt
     6601 tttctttgta ctgcaaaagt gtgtaataca tcagcagtgc agcagtc
//
LOCUS       DAOSNB010000137         6629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11665_length_6629_cov_4.186796, whole genome shotgun sequence.
ACCESSION   DAOSNB010000137 DAOSNB010000000
VERSION     DAOSNB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11665_length_6629_cov_4.186796"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1238
                     /locus_tag="PLG42_06610"
     CDS             192..1238
                     /locus_tag="PLG42_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOU30915.1"
                     /translation="MRTSKILTAVLFFFAPLLCNGQQGEPVEIKFVRNDAARKVDVYA
                     GDKLLTSYCWPENVYKPILYPVYTSAGTEITRGFPLRPREGERNDHIHQVGIWLNYGK
                     VNGYDFWGNGSRGVKEPGGGEIKHLDIEKAEGGKGEGVLVYRASWLIPSGYELLSEKT
                     EYHFIAKGTLRIIDRITTLTANDSSVVFADTKEGMFGIRVARPLELPVNETITVLDAS
                     GKPSGKKIKASEGATGNYQGSNGMSGESVWGTRAAWMHLYGKIGEEKISIVICDHPRN
                     PGYPTYWHARGYGLFAANPFGWKDFTGGKEQLNFTMKPHQQIIFRYRVIIGSGNFLTD
                     SDINSLAADFYKKY"
     gene            1330..2226
                     /locus_tag="PLG42_06615"
     CDS             1330..2226
                     /locus_tag="PLG42_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOU30916.1"
                     /translation="MKRNYSRREFIQCSIGAGALLAIAGRLPDPGRVVPSGMKLGFVT
                     YQWGRDWDLPTLISNLEKTGYYGVELRTQHAHKVESNLTPAQRAEVKKRFADSPVKCL
                     GYGSNFEYHSPDPKEVRKNIEQTKEYIILCHDIGTTGIKVKPNYLPPEVPKEKTIAQI
                     AASLNECGKFAQDYGQLVRVEVHGTYTQEIPNMKAIFEQVTEKNVVMCWNCNDQDLLP
                     PGLEANFNSVKKWFGDTVHIRELNEGNYPYQQLFDLFTSIKYEGWILLEARTEPADRI
                     AAMNEQRELFNRMIENSRKKFS"
     gene            complement(2245..5337)
                     /locus_tag="PLG42_06620"
     CDS             complement(2245..5337)
                     /locus_tag="PLG42_06620"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30917.1"
                     /translation="MRKAFKMLSGVEKIIFVTIFSIFSSFLSNAQYFGRTKPSYRVFN
                     YSVYQTPSFEIYHYLNNDSVLNLIAGRSEAWYRHHQAIFRDTIKERNPLFIYENHAEF
                     QQTSTVSDLLSESTGGVTEALKKRVVMPLTSSFPQTDHVLGHELVHAFQYNMLTTDDS
                     LKLTNVSNIPLWMIEGMAEYFSLGSIDPHTAMWMRDAVLSNDIPTLKDLTKSNKYFPY
                     RYGHSVIAFIGKTWGDSTIIKLFRETAMHGYEEAFQRILGFDDRNVSGLWNLALRNAY
                     KPLLTDTTDRITGRKILFSKNAGTMNLSPSVSPDGKYIVFTSERDVYDLDLFLADANT
                     GKIIRKLSNRINRSDIDALNYLESSGTWSPDGKFAFVVFSEGRNKLIIFDIRRMSIIK
                     ETPLTGLPAFTNPSWSPDGRYIAVTGMKNGVSDIYLYDTSNETVRQLTSDVYGNLQPS
                     WSPDSKYIVYVTDCPLPGQTHTFRHTFYNIGIVNVNNPSDDRLLELFTGASNMNPVFS
                     KDGREIYFLSDRDGFRNLYRYSLEENKIWQLTKYMRGITGITFQAPAISVARETGDIV
                     YSYYSERSYSVYRAQPDEFKAEEVDKKDIDFTAGTLPPFEHVAQNLVDSSLFSPYNYF
                     ADVKPEEYKTIPYKPKFKLDYITNGTIGVTTGGPVGTGMEGSISAIFGDMAGDNQVFT
                     SLSLNGEIYDFGGVVSYLNQKRKLKWGASLSHIPNYYGYMSAGIDTLYSRSGEPFLVN
                     TINLNYLRMFEDNLTLFAYYPLSQTRRFEVSGMISGYSYRFDQYKTYYDMFGVPVGTG
                     RKKLEAPDGFALGLLSGAFVGDNSIMGTTGPVRGERYRFEAGKYFGTIDFYTTLADYR
                     KYFYMRPFTLAFRSYNAGRWGSNAETDMVTPLYIGYPWLVRGYQRTDRYWSDAEITGE
                     GIGLSNFFGSKIFIANVELRLPFTGPKKASLIKSDLFMTDISLFTDAGLALSKGTRIG
                     RDWFNPGLNERVPVFSYGLSFRINLFGYLVLEPFYAVPVRPGAGLFSGSLGINFTPGW
                     "
     gene            5662..>6629
                     /locus_tag="PLG42_06625"
     CDS             5662..>6629
                     /locus_tag="PLG42_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU30918.1"
                     /translation="MKLKNLFFLEIFFCFFLLSCGRNTGRVFYISASGDDEAAGTLNR
                     PWKTIERVNQENLRAGDVLLFEGGSVFKGTLLIDSLYLTDDNSLITISSFGTGRAVID
                     GENSCGIHIKNASCFIIRNLEIRGNGRTGGNVSDGILIENSRDFYLDSLDVSGFQHSG
                     VHLRDCRNARLTHISAHDNGFAGIHVTSASSNHPSEYGNENIYIGYCIAFNNPGDPTV
                     KNNHSGNGILASSVKGGTIEYCEAYNNGWDMPWTGNGPVGIWIWDCTNFVIQYCVSHH
                     NKTNPVAKDGGGFDLDGGVSNSVIHYCLSYCNQGAGYGLYEFGAAKP"
BASE COUNT         1957 a         1347 c         1462 g         1863 t
ORIGIN      
        1 aaaaccatac gttgtaccgg aggttgtctg aagcgtgttg tctgagctgg aaaaaacatc
       61 ttcatttcag tttcatataa agatctgaaa tagctgttgt ccagctgatt aaacaagaac
      121 aattcatgag gggcgccgaa ggaatgaatt ttcagcaggc gccctgactt cctttaagaa
      181 aaagattcca tatgagaacc agtaaaattt taacggctgt tttattcttc tttgctcctt
      241 tactgtgcaa tgggcagcag ggtgaaccgg ttgagataaa attcgtcaga aatgatgctg
      301 caagaaaggt tgatgtttat gcaggcgaca aactgcttac atcatactgt tggcctgaaa
      361 atgtatataa accgatactt tacccggttt atacttctgc cggtacggag ataacaaggg
      421 gctttcctct tcggccaagg gagggtgaaa gaaatgatca catccaccag gtcgggatat
      481 ggcttaacta tgggaaggta aacggatacg atttctgggg gaatggttca agaggggtaa
      541 aagagccggg cggaggagag attaaacatc ttgatataga aaaggctgaa ggaggaaaag
      601 gtgagggggt actcgtttac agagcgtcat ggcttatccc ttctggttat gaattgcttt
      661 cagagaaaac tgaatatcat tttattgcaa agggaacact gcgtattata gacagaataa
      721 ccacactgac ggcaaacgat tcgtcggtgg ttttcgccga caccaaggaa ggaatgttcg
      781 gtataagggt tgcgcgccct cttgagctgc cggtaaatga aactattacg gtgctggatg
      841 cgtcaggcaa accttctgga aagaaaataa aagcctctga aggagcaaca ggaaattatc
      901 agggaagcaa tggcatgtcg ggcgaaagcg tttggggtac acgtgctgca tggatgcatc
      961 tctatggaaa aataggagaa gagaaaattt ctattgtgat atgcgaccat ccccgcaatc
     1021 ccggttatcc tacatactgg catgcaagag gttacggact ttttgcagcc aacccattcg
     1081 gatggaaaga ttttaccgga ggtaaagaac aactaaattt tacgatgaaa ccgcatcaac
     1141 agattatatt cagataccgg gtaataatag gttcaggcaa cttccttacc gattccgata
     1201 taaacagcct tgccgccgat ttctataaaa aatactgaga taatagtatg aggatgaatg
     1261 atttgtattg ttaatgaaat tcttgtaact aattgattat ttttgtttaa ccaaaaaaga
     1321 tattttgaaa tgaaaaggaa ttattcaaga cgtgagttca tacagtgtag cataggtgcc
     1381 ggggctctgc ttgccattgc aggcagatta cctgatccgg gaagagtcgt tccttcagga
     1441 atgaaacttg gttttgttac ataccagtgg ggacgcgact gggatctgcc aacccttatt
     1501 tccaaccttg aaaaaactgg ctattatgga gttgaactcc ggacacagca tgcccataag
     1561 gtggaatcaa atttaacacc tgcccagcgc gcagaggtca aaaaacgttt cgccgacagc
     1621 ccggtaaaat gcctcggcta tggatcaaac ttcgaatatc acagccccga ccctaaagaa
     1681 gtcaggaaaa atatagagca gacaaaagaa tatattattc tttgccacga catcggtact
     1741 accggcataa aagtcaaacc caactacctt cctccagaag taccaaagga gaagactata
     1801 gcacagatag ccgcatcact gaatgaatgc ggaaaatttg ctcaggatta tggccagcta
     1861 gtgagagtgg aagttcatgg gacatacact caggagattc ctaacatgaa ggcaattttc
     1921 gaacaggtaa cagaaaaaaa tgtggtgatg tgctggaact gcaacgatca ggatctcctt
     1981 ccgccaggac tggaagccaa ctttaattca gttaagaaat ggtttggcga tacagtccat
     2041 atcagggaac ttaatgaagg aaattacccg tatcagcaac tgttcgacct gtttacttcc
     2101 ataaaatacg aaggatggat acttcttgag gcacgcaccg agcctgccga cagaatagct
     2161 gccatgaatg aacagaggga acttttcaac cggatgatcg agaattccag gaaaaaattc
     2221 tcgtaggtta ttcaagcctc agatttacca tcccggggtg aaattgattc ccagagatcc
     2281 gctaaacaaa ccagcaccgg gacgcaccgg tactgcatag aacggctcga gcacaagata
     2341 accgaacagg ttaattctga aagagagacc ataactgaat accggaactc tttcattcaa
     2401 gccggggtta aaccagtctc tgcctatacg tgttcccttg cttagtgcaa gaccagcatc
     2461 ggtgaagaga ctgatatcgg tcataaataa atctgacttg ataagcgaag cttttttggg
     2521 tcctgtaaat gggagacgta gttcaacatt ggctatgaaa attttgcttc caaagaaatt
     2581 actcaagcca atgccttcac cggtaatctc tgcatcgctc cagtaacggt ctgttctctg
     2641 gtaacccctt accagccatg gatatcctat gtacagagga gtgaccatgt cagtctctgc
     2701 attgcttccc caccggcctg cattgtacga cctgaaggca agtgtgaaag gcctcatgta
     2761 aaaatacttc ctgtaatcag ccagagtggt ataaaaatca atagttccga aatattttcc
     2821 tgcttcaaac ctgtatcttt cccctcttac cggacctgtt gttcccataa tactattgtc
     2881 gcctacaaag gcaccactta aaagccccag tgcaaaacca tccggcgctt cgagtttctt
     2941 acggcctgtt cctacaggta ctccaaacat gtcataatat gttttgtact gatcgaacct
     3001 gtagctgtaa cctgaaatca tgccggatac ttcaaatctt cttgtctgcg acagcggata
     3061 atacgcaaaa agggtaagat tatcctcaaa catccttaga tagttcagat ttatagtgtt
     3121 taccaaaaat ggctctccac ttctgctgta aagagtgtcg atgccagccg acatatatcc
     3181 ataataatta ggtatatgtg agagggatgc cccccatttc agttttcttt tctgattcag
     3241 ataggaaacc actccaccaa aatcataaat ttctccattc aacgagaggg acgtaaaaac
     3301 ctgattatca cctgccatat cgccgaatat ggcactaatg cttccttcca ttcctgttcc
     3361 taccggacct cctgtagtaa cccctatagt tccgttggtg atatagtcga gtttgaattt
     3421 cggcttataa ggaatagttt tatattcttc tggctttaca tcggcaaagt aattataggg
     3481 tgagaagagc gagctgtcaa caaggttctg ggcaacatgc tcgaatggcg gcagagtgcc
     3541 tgcggtaaaa tcaatgtctt ttttatcaac ctcttcagct ttgaactcgt ctggctgcgc
     3601 cctgtaaacc gaatagctcc tttctgaata gtaagaatag acgatgtctc ctgtctcacg
     3661 ggcaaccgag attgcaggcg cctgaaaggt aataccggta atgcctctca tgtattttgt
     3721 gagctgccag attttatttt cttccagact gtatctgtaa aggttgcgaa acccgtccct
     3781 gtcagacagg aaatatattt ccctgccatc ttttgaaaag acggggttca tgttacttgc
     3841 acccgtgaaa agttcaagaa gtctgtcgtc ggaaggatta tttacattca caatcccgat
     3901 attgtagaat gtgtgcctga aagtgtgagt ctgccctggt aaaggacagt cggtaacata
     3961 aactatatat ttactgtcgg gcgaccacga aggttgcagg ttgccgtata catctgaagt
     4021 gagctgcctg actgtctcat tactggtgtc gtacagatag atatccgata ctccgttttt
     4081 catgcctgtt acggcaatgt accgtccgtc gggtgaccat gatggatttg tgaatgctgg
     4141 cagacctgta agcggcgttt cctttataat gctcatacgc cggatatcga aaattatcag
     4201 tttatttctg ccttcggaga aaacaacaaa ggcaaacttt ccatcgggcg accaggtccc
     4261 tgacgattca agatagttta aggcatcgat atcacttctg tttattctgt tcgaaagttt
     4321 tctgattatt ttaccggtat ttgcatcagc aagaaataga tcaagatcgt aaacatctct
     4381 ttctgaagta aatacaatat attttccatc aggacttaca gaaggagata gattcattgt
     4441 tcctgcattt ttgctgaaaa ggattttcct gcctgtaatt ctgtcagttg tatctgtcaa
     4501 aagtggctta tatgcatttc tgagtgccag gttccataag cccgatacat ttctgtcgtc
     4561 aaatccgaga atacgttgaa aagcttcttc ataaccgtgc attgcggtct ctctgaagag
     4621 ttttattata gtcgaatccc cccacgtctt gccaataaat gctataacgg aatgtccata
     4681 cctgtaaggg aaatacttgt tgcttttggt aagatctttt agtgtgggaa tatcatttga
     4741 cagtacagca tctctcatcc acatggcggt gtggggatct atacttccaa gtgagaaata
     4801 ttctgccatc ccttcaatca tccagagcgg aatattactg acatttgtaa gcttcaggga
     4861 gtcgtcggtt gtcagcatgt tgtattggaa tgcatgcacc aattcatggc caagaacgtg
     4921 atctgtctgg ggaaatgatg aagtcagtgg catcacgact cttttcttca gagcttctgt
     4981 aacaccaccg gtgctttcac tgagaaggtc tgagactgtt gaagtttgct ggaattcagc
     5041 atggttctca tagatgaaca aagggttacg ctcttttata gtatctctga aaatagcctg
     5101 gtgatggcgg taccatgcct cactccttcc ggcaatgaga ttcagaaccg aatcattatt
     5161 cagatagtga tatatctcaa aagacggggt ctgataaaca ctgtaattga aaactctgta
     5221 cgatggtttt gtgcgtccga aatactgggc atttgataaa aatgaactga aaatggaaaa
     5281 tattgtgaca aaaatgattt tttcaacccc cgataacatc ttaaatgctt ttctcataat
     5341 aacctccttt ctttctataa tttattaata aggaatgttt aatgatattc agcaaaatga
     5401 tgataccaga ttttcatgcg ctgcacaaat gattatcaaa ttaagtgcca gaaagctttg
     5461 ccattggttg atttttgatt gtgacctttg gggttattcg tttttgctat ccttattcca
     5521 aatcatacgc tagcacgaaa agaattagtc ttgaaatatt tttaaggaaa cagaagataa
     5581 gttgaaagaa catatttctt ggtatctgtt tttttcagaa atttaaattt gattttcatg
     5641 taaaaacaat atgaaatttc tatgaagctc aaaaacctgt tttttctgga aattttcttt
     5701 tgtttcttcc ttttaagttg tggtagaaat actggcagag tattttatat aagcgcttca
     5761 ggtgacgatg aggctgcagg cactcttaac cggccatgga aaacaattga aagagtgaac
     5821 caggagaatt taagagcggg tgatgtgctc ctgtttgaag gtggctctgt ttttaagggt
     5881 actctattga ttgattcatt gtatcttaca gatgataata gtttgattac aataagttct
     5941 ttcggcactg gcagagctgt gattgacgga gaaaactcct gtggaatcca tatcaaaaat
     6001 gcatcatgtt ttattatcag gaatcttgag ataaggggga atggcagaac aggaggaaat
     6061 gtatctgacg ggattttgat tgaaaacagc agggattttt atctcgactc tcttgatgtg
     6121 tcaggctttc agcattcagg cgtccatcta agggactgca gaaatgccag gctgacgcat
     6181 atctcagcac acgacaacgg atttgcaggg attcatgtaa cttcagccag ttccaatcat
     6241 ccttctgaat acggaaacga aaacatatac ataggatatt gcatagcttt caataaccct
     6301 ggtgacccca cggtaaaaaa taaccacagt ggaaacggca ttctggcatc ttcggtaaag
     6361 ggcggaacaa ttgaatactg cgaggcttat aacaacggat gggatatgcc atggacgggc
     6421 aacggccctg taggaatatg gatctgggac tgcaccaact ttgtaataca gtattgcgtc
     6481 tcgcatcata acaaaacaaa tccggtagca aaagacggcg gaggatttga cctcgacggt
     6541 ggtgtgtcga actcagtaat acattattgc ctctcgtact gtaatcaggg ggctggctat
     6601 gggctctatg agttcggagc agcaaaacc
//
LOCUS       DAOSNB010000138         6599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11737_length_6599_cov_4.697127, whole genome shotgun sequence.
ACCESSION   DAOSNB010000138 DAOSNB010000000
VERSION     DAOSNB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11737_length_6599_cov_4.697127"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..2568)
                     /locus_tag="PLG42_06630"
     CDS             complement(373..2568)
                     /locus_tag="PLG42_06630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOU30919.1"
                     /translation="MTISTRPLDKYAFLFAGENNDQYRADLRKVLETLIYWYGYIPEK
                     VFVRVPFPVTSLTDSVFTIAGLNLEEITGVDVNGLQSSLEAEIHSFIDEAKVLDADIT
                     PGELNTAFFYFTGPGRINPVNSKFELQIGTIDTTPVYINPLWLKNSIYDYDEQALRDS
                     SFIHIFMQQSYGFGFWDVFDQLTDSDLRMTFTAACNSGAANPSLSAGSPFTDYWTRAL
                     QFEQRDSTGNGDMIYADQEDTAGLEPTTNHLISLYKAYLFAKSFTASITAPQYKLKMP
                     AGDPADQYLGLPSLWIRDGDEPIPPETPDYWWESKDIFLTHPEATDPDARDDIYHADK
                     QNIIHVVIRNNGTHPVREFWSGTLVFLSGGGGTGDSVVNIHDIILKPGEAYEHVYPYN
                     FTGTQIHRCVRSRASIEKITLEQLDQDGIDDIQWLIADRSCEAQRNLDQQSDTGKGGT
                     GTGNTVPEPGEGKANEAKPEEEAKPPVDESGNEVQPEGTTESKSTSNLRNFREHTIII
                     RNVFRRKRKFIIAPPDEFEKLFRYCRIEICEKNNKDGRYRILKPDGKKGCVLEFDLKA
                     GETKELIIYTSLKPTKLFNHALKLPFEIYVETASVRKRIEKILKRTVDEKEIPDFMSF
                     SGFTVELCRAKGATLHGTVFNAKRKPLPGAFVYIWSGDRNQSAIVRTNKFGTYCVKDI
                     NPDNYIIQARTEDFVSKEKIIHLGDGTGTRVDFFVTAEKESIKRVRRKH"
     gene            complement(2580..4709)
                     /locus_tag="PLG42_06635"
     CDS             complement(2580..4709)
                     /locus_tag="PLG42_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOU30920.1"
                     /translation="MKTRFLFSFILTLSSALCALSQTPQGFNYQAIARDGATGNPIIN
                     QSLPVRLTIQSDSLSGIIWQEVHASVTTNSFGLFNVIVGKGTRQASSTVPKFSDIDWK
                     VTPRFIMTEVYYDGEWKLMGSSRLWSVPYSMTAAELTGSVKKLTVAGETSNMEEALFE
                     VKNKEGKTVFAVYNEGVRVYVDDGISGKGVKGGFAIGSFDSSKGIYQDYFIVTPDSIR
                     AYINTDTLKGVKGGFAIGGFDPSKAVREEFLRVTRDSTRVYLNNTGTKGRKGGFAIGS
                     FDDSKGGLIDYMLVSRDSIRLYIKEEQKGVKGGFAIGSFDDSKAGKIQYLSVSTNRTN
                     VQVKDTILGFSVTNVQSTGAADFMRIDKLNAFIGHETGVKVTPSSLGSQGKYNVFLGY
                     QSGNSNTEGYRNVFMGYRSGMKNTIAEDNVFIGTETGINNQTGNANTFIGYRAGFSTT
                     TSENTFVGHGTGYYNESGKYNTFLGKGAGSFHESGDYNVFLGWGAGNNNGYGSKNVFI
                     GAGAGNNNSGSSNIIIGFSADVSGSNNLWINNGFSTTPLISGNFSTRKVIINGKAGDF
                     TDPGYEFYVVGDAGGSTPWSSLSDERLKTNIIAINDALNKVTRIRGVSFDWIPEAGMD
                     EKHSVGIIAQELQQVMPEAVKSSGKYLSVQYGLVSALLVEAIKEQQKIIDDQKKQISE
                     LNKKLGELQITVEEMKNVVNVIKSNNY"
     gene            complement(4904..6037)
                     /gene="nspC"
                     /locus_tag="PLG42_06640"
     CDS             complement(4904..6037)
                     /gene="nspC"
                     /locus_tag="PLG42_06640"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOU30921.1"
                     /translation="MDYSKIPSPCYVIDEERFRKNLSLIRYVAEESGAEIILAFKGFS
                     MWGVFNILREYISGAAASSLNEARLCFEEIGTRAHTYSPVFKEKEFPEILRYSSHITF
                     NSLQQYRKYIHQVKKSPVKVSPGIRINPEFSEISVGLYNPCSPGSRLGVTAEALSTGL
                     PEDIEGFHFHVLFESDSYALEKVIKVVEKKFSKYFPYIKWINMGGGHLITREGYDVEH
                     LIGLLKKFRDKWGLKVILEPGSAFAWETGELVATVEDVVENSGIKTAMLDVSFTAHMP
                     DCLEMPYKPKILGAKEPPEGKFIYRMGGNSCLSGDFMGDWGFDYELKPGDRIVFWDMI
                     HYTMVKTTTFNGVQHPSIGIWTKQGKFKLIRSFSYLDYKSRLS"
     gene            complement(6087..>6599)
                     /locus_tag="PLG42_06645"
     CDS             complement(6087..>6599)
                     /locus_tag="PLG42_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOU30922.1"
                     /translation="HEELESLVKNFPTLKRARFWMTFSQEYLTHLRVIQNIGMAGIEP
                     VWFEGREIIPIRFLKAVLPDPGDLGKDYKGETSIGCRIRGIKDGKEKTYYIYNNCSHE
                     AAFSETGTQAVSYTTGVPATIGAMMYLKGIWKKPGVYNVEEFDPDPFMEQLNKLGLPW
                     VELHDVDLEL"
BASE COUNT         1783 a         1455 c         1244 g         2117 t
ORIGIN      
        1 tcagggttta tgtgaccaat tgcgccggga atgaaggttt cgttcttaaa taagcctccc
       61 ggcccggcgc tattgattgt ttgcaatgtt ttgctacaaa cagttcgctc ctacagagct
      121 ggtagttcag aaattaattg ctagctagcc ccctctcagg ggtctggggg tgaatccttg
      181 acttcaggta tgcctccttt tatttccatg tgccgtcaag ttatacattt tatttctttt
      241 ttataacttg acactgttta ttaaatactc tcacaaaatc gcgaataaac cttacaataa
      301 aatctgaaag caatcctgat atagtaatta caaaatcagt tgatgcaaaa aaacaattca
      361 ggtaattggt ttttaatgtt tccttctgac tctctttatg gattcctttt cagcagtaac
      421 aaagaaatcg actcttgtgc ctgtaccatc cccgagatga ataattttct cctttgatac
      481 aaagtcttct gtcctcgctt gaataatata attatcaggg ttgatatctt ttacgcaata
      541 tgtgccaaat ttgtttgtcc tgacaattgc cgactggttc ctgtcacctg accagatata
      601 aacaaatgca cccggtaaag gttttctttt agcattgaac actgtaccat gcaatgttgc
      661 tcctttagct ctgcagagtt ccactgtaaa acctgagaaa gacataaaat caggaatttc
      721 tttttcatca acggttcttt tcaagatttt ttcaatcctt tttctcaccg atgccgtttc
      781 gacataaatt tcaaaaggca gttttaaagc atggttaaac agctttgttg gtttcagaga
      841 ggtatatata ataagctctt ttgtctcacc agccttaaga tcaaattcta gtacacaacc
      901 ttttttacca tcaggcttca gaattctgta acggccatct ttattgtttt tttcacaaat
      961 ttctatcctg caatatctga aaagtttctc aaactcatct ggcggggcaa taatgaactt
     1021 tctttttctt ctgaaaacat ttcgtattat tatagtatgt tccctgaaat ttctaagatt
     1081 actggtagat tttgattctg ttgtcccttc cggctggact tcgtttccgc tctcatcaac
     1141 cgggggtttt gcttcttctt ctggttttgc ctcgttagcc ttcccctctc ccggttcagg
     1201 cactgtatta cctgtccccg tacctccttt tcctgtatca gactgctggt caagatttct
     1261 ttgggcctca cagcttcggt cggcaataag ccactggata tcatctattc catcctggtc
     1321 aagctgttca agggtaattt tttcaatact ggcccttgat cttacacacc tgtgaatttg
     1381 ggtacctgta aaattgtacg gatatacatg ttcatacgct tcaccgggtt ttaaaattat
     1441 atcatgtatg ttgacaacac tatctccagt tccacctcct cctgaaagaa atacaagtgt
     1501 tcccgaccag aactcacgaa ccgggtgggt gccattattg cggataacaa catgaataat
     1561 attctgttta tctgcatggt agatgtcatc tcttgcatca gggtcagttg cctccggatg
     1621 tgtcagaaaa atatccttac tttcccacca gtaatcgggc gtttcaggtg gaattggttc
     1681 gtccccgtcg cgtatccata aggaaggcaa cccaaggtac tggtcggcgg gatcgccggc
     1741 aggcattttc aatttatact gaggagctgt aattgatgca gtaaaagatt tagcaaaaag
     1801 ataagcttta tataacgaaa taaggtggtt tgtcgttggt tcaagccctg ctgtgtcttc
     1861 ctgatcggca tatatcatat ctccgttacc agtactgtcc ctctgttcaa actgaagtgc
     1921 tctggtccag taatctgtaa atgggctacc tgctgaaagc gaaggattag ctgctccgga
     1981 attgcatgca gcagtgaatg tcatacgaag atcagaatct gtaagctggt cgaatacatc
     2041 ccagaagcca aagccatacg attgctgcat aaaaatatgg ataaatgaac tgtctctcaa
     2101 agcctgttca tcataatcat aaatggagtt ttttaaccat aatgggttaa tatatacagg
     2161 ggttgtatca attgtaccaa tctgcaattc aaactttgaa tttaccggat taatccgccc
     2221 cggtcctgtg aaatagaaaa atgctgtatt aagttcgccg ggagtaatat ctgcatcaag
     2281 cactttcgcc tcatcaataa aactatgaat ctctgcttca agtgatgatt gaagtccatt
     2341 tacatcaaca cctgtgattt cctcaaggtt gagtccagcg atggtgaata cactatcggt
     2401 tagagatgtg acaggaaaag gtactcttac aaataccttt tcagggatat atccatacca
     2461 gtaaataagt gtttcaagga ctttcctcag atcagccctg tactggtcgt tgttctctcc
     2521 tgcaaacagg aaagcgtact tatcaagtgg acgagttgaa atagtcatga tttctttaat
     2581 taataattat ttgatttaat aacatttaca acatttttca tttcttcaac agttatttgc
     2641 agttcgccaa gtttcttgtt cagttctgag atttgctttt tctggtcatc aataattttt
     2701 tgttgttctt ttattgcttc aaccagaagg gctgaaacca gaccatactg tactgaaagg
     2761 tattttccgc tggattttac tgcttctggc ataacctgct gaagctcctg tgcaattatt
     2821 ccaacgctgt gtttctcatc cattccggct tccggtatcc agtcaaaact tactccgcga
     2881 atcctggtaa ctttattcaa tgcgtcattt attgcaataa tgttggtttt gaggcgttcg
     2941 tcgctcagac tactccaagg agttgaaccg ccggcatctc caacaacata aaactcatag
     3001 cctggatcgg tgaaatcacc tgccttaccg ttaattatta cttttcgtgt agaaaagtta
     3061 cctgaaatca aaggagttgt gctgaatcca ttatttatcc acagattgtt actgccagaa
     3121 acatcggcgc taaaacctat aattatattg ctgcttcctg aattattatt accggctcca
     3181 gctcctataa acacattctt gctaccatat ccattgttgt tgcctgctcc ccagcccaaa
     3241 aaaacattat aatcgccaga ttcatggaat gaacctgccc ctttgccaag aaaagtgttg
     3301 tatttgcctg attcgttgta atagccggta ccatggccaa caaaagtatt ttcagaagtg
     3361 gtcgttgaaa atcctgccct gtatccaata aaagtatttg catttcctgt ctggttattt
     3421 atgccggttt cagttccaat aaaaacattg tcttcggcga tggtattctt catacctgac
     3481 ctgtaaccca tgaaaacatt tctgtagcct tcagtattac tgtttccgct ctggtatccg
     3541 aggaaaacat tgtatttacc ctggcttcca aggctgcttg gggttacttt cacaccggtt
     3601 tcatgaccta taaatgcatt aagcttgtct atcctcataa aatctgccgc acctgtgctc
     3661 tgtacatttg ttacagaaaa gccgaggata gtatctttaa cctgtacatt tgttctgttt
     3721 gtactgacac taaggtactg gatcttgcct gctttgctat cgtcgaaact tccaattgca
     3781 aatccgcctt ttactccttt ctgttcctct ttaatataga gcctgatact gtcgcggctc
     3841 acaagcatat agtcaataag tccgcctttt gaatcgtcga agcttccgat agcaaatccg
     3901 ccttttcttc cttttgtgcc ggtattattc agataaacac gggtgctatc acgtgtaact
     3961 ctcagaaatt cttcccttac agctttggaa gggtcaaatc cgcctattgc aaaccctccc
     4021 ttaactcctt ttaatgtatc ggtattaatg tatgctctta tactgtcggg tgttacgata
     4081 aagtaatcct gatagattcc tttcgatgaa tcgaaactgc cgattgcaaa tcctccttta
     4141 acaccctttc ctgaaattcc atcatcaaca taaactctaa ctccttcgtt ataaacagca
     4201 aaaactgttt taccttcttt gttctttact tcaaataatg cctcctccat atttgatgtt
     4261 tcacctgcta ctgttaattt cttaacagaa cccgtgagct ctgcagctgt cattgaataa
     4321 ggcacagacc acagtcgtga cgagcccatt aatttccact caccatcgta atatacttct
     4381 gtcataataa acctgggcgt taccttccag tcgatatccg aaaatttcgg aactgtcgat
     4441 gatgcctgac gtgtaccttt gccaactata acattaaata gcccaaatga attggtcgtt
     4501 actgatgcat gcacttcctg ccatattatc cctgaaaggg aatcggattg tatcgtcagt
     4561 cgtacaggga gtgactggtt gattattgga ttacctgttg ccccgtcgcg ggcaatggcc
     4621 tggtaattaa acccctgcgg ggtttggctc agggcgcaga gcgcagagct cagggttaga
     4681 atgaatgaga aaagaaatct tgttttcata acaatctatt ttagcggctg gatggctcag
     4741 gggatcagcg aaacagataa ccttgggaca agaagcctgt tgttaaaaaa tattgagaat
     4801 taacttatta tgcaagttaa aataaataat aatcgaaaac aactatctta gaaaaaaaat
     4861 gaaaagaatt acagaaaatt tttagaaagc ttttagtaaa aaatcaggag agtcgtgatt
     4921 tgtagtcaag atatgaaaaa ctacgaataa gtttaaattt tccctgtttt gtccagatgc
     4981 ctatagatgg gtgctggacg ccattgaatg tggtcgtttt gaccatggta taatgaatca
     5041 tatcccagaa gactattctg tcgcccggtt tcagttcata gtcgaatccc caatcgccca
     5101 taaaatcgcc tgaaagacat gagtttccgc ccattctgta aataaatttt ccttctgggg
     5161 gttcttttgc tcccagaatc tttggcttat aaggcatctc aaggcaatcg ggcatatggg
     5221 ctgtaaatga gacatcgagc attgcagttt ttatcccgct attctcaact acatcctcaa
     5281 ccgttgcaac aagctctcca gtttcccagg caaaagcact cccgggctcg agtataacct
     5341 tcagtcccca tttatccctg aattttttaa gcaatcctat aagatgttca acatcatagc
     5401 cctctcttgt aataagatgg cctccgccca tattaatcca tttaatataa ggaaagtatt
     5461 tactgaactt cttttccacc acctttatta ccttctcgag agcatatgaa tcgctttcga
     5521 aaagcacatg gaaatgaaaa ccttcaatgt cttcagggag gcctgttgat agggcttcag
     5581 ccgtgacgcc aagtctcgag ccaggtgaac atgggttata caaaccaacg ctaatttcag
     5641 aaaattccgg atttattctt attcccggag acactttcac aggtgacttt ttaacctgat
     5701 gtatgtattt cctgtattgt tgaagtgaat tgaaagtaat atgggagctg tatctaagaa
     5761 tttcaggaaa ttccttttct ttaaatacag gggagtaagt atgtgcccgt gtaccgatct
     5821 cctcgaagca gagtctggct tcgtttaatg agcttgccgc ggcacctgat atatattcgc
     5881 ggaggatatt aaacacaccc cacatagaaa atcccttgaa tgcaagaatt atttcagcac
     5941 ctgattcttc tgccacatat cttatgagtg aaagattttt tctgaatctt tcctcgtcaa
     6001 tcacatagca gggagatggt atttttgagt agtcaattga tgccacgggg ttcgaggttc
     6061 aaggtttgag gttcaaagtt cgaggtttaa agttcgaggt caacatcgtg cagctctacc
     6121 catggaagac caagtttatt gagttgttcc ataaacgggt cggggtcaaa ttcttcaaca
     6181 ttatatacgc cgggtttttt ccagatgcct ttcaggtaca tcatagcccc aatggttgca
     6241 ggtacgccgg ttgtgtagct tacagcctgt gtcccggttt cggagaaggc tgcttcgtgg
     6301 ctgcaattgt tatatatata atacgtcttc tcttttccgt ctttaattcc ccttatacgg
     6361 catcctatag aggtctcgcc tttgtaatcc tttccaaggt caccagggtc aggcagtacg
     6421 gctttgagaa atctgattgg aataatttcg cgtccttcaa accatacagg ctcaattcca
     6481 gccatgccaa tattctgtat aacacgcaga tgggtaagat attcctgact gaaagtcatc
     6541 cagaatctcg cacgtttcag cgtgggaaag ttcttaacaa gggattcgag ttcttcatg
//
LOCUS       DAOSNB010000139         6597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11745_length_6597_cov_4.095537, whole genome shotgun sequence.
ACCESSION   DAOSNB010000139 DAOSNB010000000
VERSION     DAOSNB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11745_length_6597_cov_4.095537"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..471
                     /locus_tag="PLG42_06650"
     CDS             67..471
                     /locus_tag="PLG42_06650"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOU30923.1"
                     /translation="MWFWNYFLTGFLVWLTGRAAWHIGASGLVYGLTAFLFFSGIIRR
                     YYRLAALSLLVVFLYGSMVWGMFPGFYRDVSWESHMMGFLSGIILAIYYRHEGPQRPV
                     YKWLEEEEELNETEPFSDNNENDTDKKLLENF"
     gene            771..2075
                     /locus_tag="PLG42_06655"
     CDS             771..2075
                     /locus_tag="PLG42_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30924.1"
                     /translation="MKLPTPVLRIVLLLIVMSNSLIALSQENAQNLDSLRKDALRLFL
                     DCRTCDMNYTREQIPYVNYVRDVKEADVYVLVTQQNAGSGGNMYTYTFSGQFRFAGMH
                     DTLTFTSNPDMTSAEIREKRTNLLKMGLMRYVARTPLAGEIEIRHNQRLQAAKVEDKW
                     NYWVFEIQTSPRFNAEASNSRLFFSNSLNITRITPDLKFEFEVETDNIRQKFVDDEGE
                     ATIYTRKDENMNVLLVKSMGEHWSAGSRLNLRTATNPNYDLNFEFMPAVEYDIFPYSL
                     ATHKQLRINYSAGFQYSDYIDSTLYNKTYDNLFRHELQLAYQVQEKWGSINISLTASN
                     YFHDWSKNRVQVDGFIRLRIVKGLSLSINGQAAYINDQLNLRKGELTEAERLLRLKEQ
                     ATNYSVGGSIGITYTFGSIYNNVVNPRFGYRRGGGGEFMRFF"
     gene            2155..4491
                     /locus_tag="PLG42_06660"
     CDS             2155..4491
                     /locus_tag="PLG42_06660"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOU30925.1"
                     /translation="MKPAFCFLSAAIITFVFRCPDINCQNNNSEYLIWFNKPASEWNE
                     ALPVGNGSLGAMVFGGIFNERLQLNEETVWTGEPWWDANPDARKTLPAVRKLLFEGRY
                     REAEELALKGIMGKIRRDALSSYQTLGDMLINFRHGKNISNYRRELDIQEAIARVSYS
                     AGQTYFQREIFSSAPHKALITRITASNAGSVSFSVKLTRPGNKAEIKAADGIITMKEH
                     VGDGKGVQLFAVLKIINEGGSIAASGDSLSVEKADAVTIFLTASTDYFNNNPEVTAFD
                     RMSKAEKIPYYQLKNEHIKDYSSFFKRVEIDLGSSNGKYFTTDARITAMQNGYTDTDL
                     IELYYQFGRYLLISSSRPGGLPANLQGIWADGLKPPWSADYHININIQMNYWPAEITN
                     LAELHEPFIEFINAMVPNARRTASEVYGLKGIVAHYTTDVWHYTEPFGAIQYGMWPMG
                     IAWACRHLWEHFEFGGDIKYLRLKAYPVMKEATEFCMGWLVKDPRTGYMVSGPSISPE
                     NSFIIPGNGGRASMVMGPTMDHMIIRDLLQNTIKASILLDDDIRFRRKMQQTLEKLAP
                     VRISSDGRIMEWPEEFKEAEPGHRHMSHLYGLHPGNQITSQNTPELFEAAKRTIEYRL
                     AHGGGHTGWSRAWIINFYARLKDGEKAYENVLALLTKSTLNNLLDNHPPFQIDGNFGG
                     TAGITEMLLQSHAGEIELLPALPAAWATGFVHGLMARGGFEVNIDWENGKLKQAVIIS
                     KLGNPLKVSYRGKTTELKQTVKNQSYIITEQQTELLIK"
     gene            4497..5816
                     /locus_tag="PLG42_06665"
     CDS             4497..5816
                     /locus_tag="PLG42_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30926.1"
                     /translation="MHEKFRFRNSDELLQKACQLSLNLPFSDDISPLFYPVNIGERII
                     SNRFVVQPMEGYDSEPDGSPSDLTTRRYLRYAAGGSGIIWFEAIAVTPEGRSNPRQLM
                     INTKNCERYKKLVENIRLKSPLNAQPFIVAQLTHSGRYSRPEGQPMPLVPCNHPLLDK
                     GNEKILSDDDLVRIQDYFIASAKLAHEAGFDAVDIKACHGYLIHELLFSFRRENSIYG
                     GPEPVKRFRFLLETIDRVKEEVPDIMVTTRLNISDIYPGGFSTTPDGKNYDLGEALML
                     IGELEKRGLKILNTTMGSPYFNPHVVRPYDNPLPGASKPAEHPLQGVVRMIEGTSIVQ
                     HAFSGLMVVGSAYSWLRQFALNTGAAVIKSGGATFIGFGRNSFAYPEMPRDTMTKGYP
                     ESSKFCITCSGCSRLMKNFRPAGCVIHDREIYGNELKKLIDDGKKNS"
     gene            5794..>6597
                     /locus_tag="PLG42_06670"
     CDS             5794..>6597
                     /locus_tag="PLG42_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOU30927.1"
                     /translation="MMERKIVDFEGFRLCCYSSNTLIIGSGAAALNAAIQLHMAGQKD
                     ILIVTEKWGAGTTRNAGSDKQTYYKLSFTGDEPDSARIMAEDLFKGKCMHGDIALCEA
                     QGSVQSFLNLVRLGVPFPQNRYGAWVGYKTDHDPRARGTSAGPYTSKLMFEALSGEVK
                     KRKIKILDNTRVVALLTKSGKNVREVIGALAIDLKADKPENAFILFNSVNIIMATGGP
                     AGIYRDSVYPPSQHGSTGIALEAGATAQNLIESQFGIASLKFRWNLSGSY"
BASE COUNT         2096 a         1345 c         1386 g         1770 t
ORIGIN      
        1 cgtctatctc acttaaataa aaaaatctac taactttttt atttctattc agaacctgct
       61 ttaaaaatct ggttctggaa ctattttctc acagggttcc ttgtatggct taccggaaga
      121 gcggcatggc atattggggc gagtgggctt gtctatggtc ttacagcatt tctttttttc
      181 agtggcataa tccgccgtta ctaccgtctg gctgcactat cactgcttgt agtttttctc
      241 tacggctcaa tggtatgggg tatgtttccg ggattttaca gggatgtatc atgggaatcg
      301 catatgatgg gctttttatc aggaataata cttgcaattt actacaggca cgaagggcct
      361 caaagacctg tatataaatg gcttgaagag gaagaagaat taaatgaaac agagcctttt
      421 tcagataaca atgaaaatga caccgataaa aagttactag aaaattttta agctaagttt
      481 aaaaattact tcttctccag aagccctctt ccttttgtta taataagtca aaagattctt
      541 aattatctgt ctatctatcg gttataaata atattacatt gaataactgt taaaaataat
      601 taaatcgact acgatttttt ttctggttaa gatttatttt aatccaattt ccgatatttg
      661 catgccctgt ttattaatgt tgcttattac cagcaagact attaacatta ttactttaaa
      721 actgaacaat atacagtaat ttgattttta aaataataac ttttcagatt atgaaattgc
      781 cgacaccagt tttaagaata gttctgcttc ttattgttat gtcaaattca ttaattgctc
      841 tttcacagga aaacgcgcaa aatctagatt ccttgagaaa agatgcctta agacttttcc
      901 tcgattgccg tacctgtgat atgaattaca caagagagca gatcccatat gttaactatg
      961 taagagacgt aaaagaagct gatgtttatg ttcttgttac ccagcagaat gccggaagtg
     1021 ggggcaatat gtatacttat acattttcag ggcagttccg cttcgccgga atgcacgata
     1081 cacttacatt taccagcaat ccggatatga ccagcgccga aatacgcgaa aagagaacca
     1141 acttgctgaa aatgggcctt atgagatatg ttgccagaac ccccctggcc ggtgaaatcg
     1201 aaatcaggca taaccagagg cttcaagctg caaaagtgga agataaatgg aattactggg
     1261 tatttgaaat ccagacaagt ccacgattca atgccgaagc ttcaaattca agactattct
     1321 tttcaaattc tctaaacatc acaagaatca cccctgatct caaattcgag tttgaagttg
     1381 aaacagataa catccgtcaa aaatttgtgg atgatgaagg tgaagcaaca atttacacaa
     1441 gaaaagatga aaatatgaat gtcctgcttg ttaaaagcat gggtgaacac tggtcggcag
     1501 gttcacggct taacctgcgt acagccacta atccgaacta tgatcttaac tttgaattta
     1561 tgccagctgt cgaatatgat atcttcccct attcacttgc cactcataaa cagctgagaa
     1621 ttaattacag tgcaggattt cagtatagtg attatattga ctcaacactt tacaacaaga
     1681 cctacgacaa tttgttcagg catgaactac agttagctta ccaggttcaa gaaaaatggg
     1741 gttcaataaa tatctctctt acagcttcaa attactttca cgactggtca aaaaacaggg
     1801 ttcaggtaga tggttttata cgccttcgaa ttgtgaaagg tctttccctc agtatcaacg
     1861 gacaggctgc ttatataaac gaccagctca atctgaggaa gggagaactc actgaagcag
     1921 agagactcct gcggttgaaa gaacaggcaa caaattattc agtgggaggt agcattggta
     1981 ttacttatac tttcggttca atctataaca atgttgtaaa tcctcgcttt ggttaccgcc
     2041 gcggtggagg tggtgaattt atgagattct tttaattcca cttcaaaatt cagggaaaaa
     2101 actgaattag cttccggata aaataaataa tttagtcagg ttaaatcgtt cacgatgaaa
     2161 cctgcctttt gctttttatc cgctgcaatt atcacatttg ttttcaggtg ccctgatatc
     2221 aattgtcaaa acaataattc tgaatattta atctggttca acaaaccagc atctgaatgg
     2281 aatgaagccc tgcctgtcgg caacggaagt ctgggtgcaa tggtcttcgg gggaattttc
     2341 aatgaaagac ttcagctgaa tgaagaaacc gtatggactg gtgagccgtg gtgggatgcc
     2401 aatcccgacg cccgtaaaac cctgcctgcc gtccggaaac tgttgtttga aggcagatac
     2461 cgcgaagcag aagagcttgc ccttaaagga atcatgggca aaataaggcg tgatgccctt
     2521 tccagctatc agacactcgg agacatgtta atcaatttcc ggcatggtaa aaatatttca
     2581 aattaccgca gagaacttga tattcaggaa gcaattgccc gagtgagcta ttcggctgga
     2641 caaacatatt ttcaaagaga gattttctcc agcgcccctc ataaagcact tattacaaga
     2701 atcacagcca gcaatgcggg ttcggtctca ttctcagtga aattgacacg accaggtaat
     2761 aaagcggaaa ttaaagctgc tgatggaatt atcaccatga aggaacacgt aggtgatgga
     2821 aaaggtgtac aactttttgc tgtattaaaa attatcaatg aaggaggtag cattgcggct
     2881 tcaggcgata gtttaagtgt tgaaaaagct gatgccgtaa cgatattcct tactgcttca
     2941 actgattatt ttaacaacaa tcctgaagta actgcatttg acagaatgtc aaaagccgaa
     3001 aagattcctt attatcagct taaaaatgaa cacattaagg attacagctc tttttttaaa
     3061 agggttgaaa tagaccttgg ttcaagcaat gggaaatact tcacaacaga tgcccgtatt
     3121 actgccatgc aaaacggata taccgacact gacctgattg agttgtatta tcagtttggc
     3181 cgttatctgc ttataagcag ttcccggccg ggcggactgc ctgccaacct ccagggaata
     3241 tgggctgatg gcctaaaacc cccatggagt gccgattatc atataaacat aaacattcag
     3301 atgaactact ggcctgctga aataactaac ctggctgaac ttcatgaacc atttattgaa
     3361 tttataaatg caatggtccc caatgcaaga cgaactgcca gtgaagtata tggattaaaa
     3421 ggtatagtag cgcactatac taccgatgtg tggcactata cagaaccatt tggggcaatc
     3481 cagtacggaa tgtggccgat gggaattgca tgggcatgtc gacatctctg ggaacatttt
     3541 gaattcggag gtgacataaa gtatctaagg ctaaaagcat atcctgtcat gaaagaggca
     3601 acagaattct gcatgggatg gcttgtcaaa gatccacgta ctggctacat ggtgtcaggc
     3661 ccatcaatat cccctgagaa cagcttcata ataccaggca acggaggaag agcaagcatg
     3721 gttatgggtc cgacaatgga ccacatgatt atcagagatc tccttcagaa cacaatcaaa
     3781 gcttctattc tgctcgacga cgatatcagg ttcaggagaa aaatgcaaca gactctggaa
     3841 aaactggcac cggtgagaat cagttctgac ggtcgtataa tggaatggcc agaagaattt
     3901 aaagaagctg aacccggtca caggcatatg tcacaccttt acggccttca tcccggaaac
     3961 cagataacca gccagaatac ccctgaattg tttgaagctg caaaaaggac catcgaatac
     4021 agacttgctc acggaggggg ccataccggc tggagcagag catggataat taatttctat
     4081 gcacgcctca aagacggaga aaaagcgtac gaaaatgttc tagcactgct tacaaaatca
     4141 acgctaaaca atcttctcga taatcatccg ccatttcaga ttgatggaaa ctttggagga
     4201 accgcaggaa taacagaaat gctgcttcaa agccatgcag gtgaaattga acttctgcct
     4261 gccctgccag ccgcatgggc aaccggattt gttcatggac tgatggcaag aggaggtttt
     4321 gaagtcaata tcgattggga aaacgggaaa ctaaaacagg cagttattat ttctaaactc
     4381 ggaaacccat tgaaagtctc atatagaggc aaaaccacag aactgaaaca aacagtaaaa
     4441 aaccaatcct atattataac tgaacaacaa acagaattac tgattaaata acctctatgc
     4501 acgaaaagtt cagattcaga aacagtgatg agcttcttca gaaagcatgc cagctctccc
     4561 tgaacctacc attcagtgat gatataagcc ctttgtttta tccggtcaat atcggtgaaa
     4621 gaattatcag taaccggttt gttgtccagc ctatggaggg ttatgactct gagccagatg
     4681 ggtccccctc agaccttaca accagaagat atttgaggta tgcagccgga ggaagcggaa
     4741 taatatggtt tgaagctatt gccgttacac ctgaaggacg ttccaatccc cgtcaattga
     4801 tgattaatac gaaaaattgt gagaggtaca agaaacttgt agaaaatatc agattgaaat
     4861 ctccattaaa tgcccagcct tttattgttg cccagttaac acattcaggt cggtacagca
     4921 ggcctgaagg acagccaatg cctctggtcc catgcaacca tccgttgctc gacaaaggta
     4981 acgaaaaaat actatcggat gatgatcttg taaggataca ggattatttt attgcatcag
     5041 caaaactggc acatgaagca ggtttcgacg cggttgatat caaagcctgt catggttatc
     5101 ttattcatga acttcttttt tcattcagaa gggaaaacag catttacggt ggaccagaac
     5161 ctgtaaaaag attccggttt ctgcttgaaa caattgaccg cgtaaaggag gaggtacctg
     5221 acataatggt tactacccgc ctcaatatca gcgatatcta tcctggtgga ttctccacta
     5281 ctcccgatgg aaaaaattat gaccttggag aagcattaat gctcatcggg gaacttgaaa
     5341 aaaggggatt aaagatatta aatacaacca tggggagccc atatttcaat cctcatgtcg
     5401 tcaggcctta tgataatcct ctgcccggag cttcaaagcc cgcagaacat cctttacagg
     5461 gagtggtcag aatgattgaa ggcacttcaa ttgtgcagca tgcattctcc gggctaatgg
     5521 ttgtaggttc ggcctattca tggctcaggc aattcgccct gaatacaggt gctgcagtaa
     5581 ttaaatcagg aggcgccact tttataggct tcggacgaaa ctcatttgct tatccagaaa
     5641 tgcctcggga tacaatgaca aaaggctatc cggaatcttc taaattctgc ataacctgtt
     5701 cgggatgctc aaggcttatg aaaaatttca ggccagcagg atgtgttatt cacgacaggg
     5761 aaatatacgg taacgaactg aaaaaactta ttgatgatgg aaagaaaaat agttgacttt
     5821 gaaggcttcc ggctctgctg ttacagtagt aatacactga tcataggaag tggggccgca
     5881 gcactaaatg cagcaattca gctgcatatg gcaggtcaga aagacattct catcgttact
     5941 gaaaaatggg gtgccggaac aacccgaaat gcaggttccg acaaacaaac atattataaa
     6001 ctttcattta caggtgatga gcccgacagt gcaagaatta tggctgaaga ccttttcaaa
     6061 ggcaaatgca tgcatggaga tatcgcactt tgtgaagcac agggatctgt acagtcattc
     6121 ctgaatcttg tcaggctggg tgtcccattc ccgcaaaacc gatatggagc ctgggtgggt
     6181 tataaaaccg accacgaccc aagggcaaga ggaacatctg ccggtcctta cacttcaaaa
     6241 ctaatgtttg aagcactttc aggagaggtg aaaaaaagaa aaatcaaaat actcgacaat
     6301 accagagtgg ttgctttgct cacaaaatca ggaaaaaatg taagagaagt tataggtgcc
     6361 ctcgccatcg accttaaagc cgataaacct gaaaatgcat ttattctgtt taattctgtc
     6421 aatattatta tggccacagg aggaccggca ggcatttaca gggattctgt ctatccgcct
     6481 tcacaacatg gatcaaccgg catagccctt gaagccggtg caacagccca gaatctcatt
     6541 gaatcacagt ttggtatcgc atcattaaaa ttcagatgga acctttcagg cagctat
//
LOCUS       DAOSNB010000140         6591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11762_length_6591_cov_4.451958, whole genome shotgun sequence.
ACCESSION   DAOSNB010000140 DAOSNB010000000
VERSION     DAOSNB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11762_length_6591_cov_4.451958"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PLG42_06675"
     CDS             complement(<1..824)
                     /locus_tag="PLG42_06675"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOU30928.1"
                     /translation="MTVSVVGIGLIGGSLAIDLRKRGFAERVIGVDTNIQHQNIAKLC
                     GIVDETDTLENAVEKSDLIILCTPVDSNCKLLPVILDMIGGTTKVVTDMGSTKASIAR
                     AAKDHPARGRFVAAHPMAGTEYSGPLAAHSKLFDYKMAIICDKELSDPDALETIKEML
                     NVLNMRIVYMDSASHDVHVAYVSHISHITSFSLALCVLDKEKEEENILTLAAGGFEST
                     VRLAKSSGDTWAPIFIENAEFILEVIDTYIEKMHLFRQMIVKKDAEGLKKLMDEAN"
     gene            complement(873..2033)
                     /locus_tag="PLG42_06680"
     CDS             complement(873..2033)
                     /locus_tag="PLG42_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU30929.1"
                     /translation="MKVKPAERTNSVGEYYFSAKLRQIDQMRRAGIDVINLGIGSPDL
                     PPPPEAIKKLSEEAALPGVHGYQSYNGLPELRKAMSDWYQKYFGVTLNPENEILPLIG
                     SKEGIMHISMAFVNPGDEVLVPDPGYPSYTSGTRLVGGVVRTYDLLAKNNWMPDINLI
                     ESTSLSKVKLMWVNYPHMPTGTKASAILFERLIKFAAKHGILICNDNPYSFILNSEHI
                     SILSVDGAKEYALELNSLSKSHNMAGWRVGLVAGDRDYISTVLKVKSNMDSGMFRPLQ
                     LAAAEALKRGDDWFEKNNEIYRRRREIANEIMDILGCRYDKEQTGLFLWGQVSENYKN
                     GEELSDMVLEKAHVFITPGFIFGKNGDGYVRISLCSNEEKLKEARERIKNLA"
     gene            complement(2023..2877)
                     /locus_tag="PLG42_06685"
     CDS             complement(2023..2877)
                     /locus_tag="PLG42_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOU30930.1"
                     /translation="MIKMNGHIRVSIQGETGSFHEVAAKQYFGGNNIDIVPCATFDQT
                     LAEVKTGRADFAVMAIENARAGSILYNYTLIRESGLKILGEHNLRIKQNLMALPGQTL
                     ADIKEIRSHPIAFSQCMTFLNQHPEITLVETEDTAGSARIISEKRLMGIAAIASSVAA
                     EMYGLEILAEGIETYRENYTRFLVIGSRSKGLRTGNKASICFSLNHRPGSLANVLIKL
                     AELNINLTKIQSVPRLNGKGWEYMFYLDLEIPEDTTMELIYSLLEDDTHDMEILGIYL
                     KDDKIYES"
     gene            complement(3177..3776)
                     /gene="hisIE"
                     /locus_tag="PLG42_06690"
     CDS             complement(3177..3776)
                     /gene="hisIE"
                     /locus_tag="PLG42_06690"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOU30931.1"
                     /translation="MNKIDFSKMSGLVPAVIQDKQTLQVLMVGFMDEEALNRTINEGR
                     VSFYSRTRKAQWTKGETSGNFLYVEEILPDCDNDALLIKVKPAGPVCHLGTTSCFGNA
                     PAKGFMYRLEEIINQRIYENAENSYTNKLYREGINKIAQKTGEEAVELVIEATKEDSH
                     ESFCNEAADLMYHFLVLLRAKGYKLEDIEEVLMKRHRNK"
     gene            complement(3776..4528)
                     /gene="hisF"
                     /locus_tag="PLG42_06695"
     CDS             complement(3776..4528)
                     /gene="hisF"
                     /locus_tag="PLG42_06695"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOU30932.1"
                     /translation="MLKKRIIPCLDVKDGRTVKGVNFVNIKDAGDAVELASRYVAEGA
                     DELVFLDITATIERRKTLVSLVENVARAINIPFTVGGGINSLKDVEAIINAGADKISI
                     NTAAVKNPGLITEIANRFGSQCVVVAIDTGFAENTWKVFINGGRTPTGIDTVEWAKRA
                     EDAGAGEILLTSMTADGTRSGFSIGITKKIVESVNIPVIASGGAGSKEDIRDVFLHTG
                     CSAALAASIFHYGEIKIYELKKYLSQFNIPVR"
     gene            complement(4522..5235)
                     /gene="hisA"
                     /locus_tag="PLG42_06700"
     CDS             complement(4522..5235)
                     /gene="hisA"
                     /locus_tag="PLG42_06700"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOU30933.1"
                     /translation="MRIIPAIDIIGGKCVRLTRGDFSSKKEYRGDPVDTAKMFEDAGL
                     EYLHFVDLDGAREGRVVNYRVLEKIAVTTSLIIDFGGGIRTDDDVRIAFECGASQVNC
                     GSLAVLNREMFLEWLKKHGTERIILSADTRNRKVCVEGWTSEKDEDIIDFISRYSLEG
                     VSYVVCTDIERDGMLEGPAIELYKDILSKCKINLIASGGIRSVNDLYRLSETGCEGAI
                     LGKAIYEGYIQLKDLAELC"
     gene            complement(5232..5810)
                     /gene="hisH"
                     /locus_tag="PLG42_06705"
     CDS             complement(5232..5810)
                     /gene="hisH"
                     /locus_tag="PLG42_06705"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOU30934.1"
                     /translation="MIAILKYNAGNIKSVINALARAGHEAIITDSKEELVRADKVIIP
                     GVGHAGQAMKYLKERGLDRVILSLKQPVLGICLGLQLMCISSEEDNTECLGIFNSKVR
                     KFPPLQVVPHMGWNNFISIYGKLFDGLKESDDMYYVHSYYAETGESTVAVCEYILPFS
                     AAMQKDNFYATQFHPEKSGDKGEKVLRNFLSL"
     gene            complement(5807..>6591)
                     /gene="hisB"
                     /locus_tag="PLG42_06710"
     CDS             complement(5807..>6591)
                     /gene="hisB"
                     /locus_tag="PLG42_06710"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOU30935.1"
                     /translation="RKPGTALLTKYLSQGIDLQSSYVIGDRLTDVELAKNLGCKAILF
                     GGRKSDYASLCTSDWDEIFRYITGLNRKAIEEYITGETKITAEVNLDGTGKIEADTGI
                     GFFNHMLSQIARHGNIDIKLKAEGDLDVDEHHTIEDSAIALGRAVARALASRKGIERF
                     GFTVPMDDSLANVAIDLSGRPWLVWDVTFTREKIGDMPSEMVFHFFKTFADNAGCNIN
                     VKAEGSNEHHKAEAIFKAFGKALGMAVNKTGRGDIPSTKGKL"
BASE COUNT         1627 a         1603 c         1249 g         2112 t
ORIGIN      
        1 ctgttagcct catccatgag cttttttaaa ccttctgcat ccttttttac aatcatctgc
       61 ctgaagagat gcattttctc aatataagta tctataactt caaggatgaa ttcggcattt
      121 tcgatgaata tgggagccca cgtgtcgccg ctacttttag caagcctcac tgtgctttca
      181 aaacctccgg ctgcaagtgt cagtatgttt tcttcttcct tttccttatc gagcacacag
      241 agagccagtg aaaaagaagt gatatgcgat atgtgcgaaa catatgccac gtgcacatca
      301 tgactggccg aatccatgta tactatcctc atgttgagaa cgttcagcat ttcttttatg
      361 gtctccaggg catcagggtc actcagttcc ttatcgcata ttattgccat tttgtagtcg
      421 aatagcttgc tgtgtgcggc aagagggcct gaatactctg tacctgccat tggatgggct
      481 gcaacaaatc ttcctcttgc aggatgatcc tttgctgccc ttgctataga agctttggtt
      541 gaacccatat cggtcacaac cttggtggtt ccgccaatca tgtcaagaat gaccggaagg
      601 agcttgcaat tagaatcgac aggagtacat aaaatgatca gatcactctt ctcaacggca
      661 ttttcaagtg tgtctgtttc atcaacaata ccgcacaact ttgcaatgtt ctgatgctga
      721 atatttgtat caacaccgat aaccctttcg gcaaatcctc tcttacgcag atctattgcc
      781 agagaaccgc ctataagacc tattcctaca acacttacag tcataatgat atttcgtatt
      841 tatatttact ttttaaaatc atatgttgct tcttaagcca ggttttttat tctctctctg
      901 gcttctttaa gtttttcttc attgctgcac agcgaaattc ttacatatcc gtcgccgttt
      961 ttgccaaaaa taaacccggg cgttataaaa acatgtgctt tctcgagtac catatctgaa
     1021 agttcttcgc cgtttttgta attttcactc acctggcccc agagaaatag gcccgtttgt
     1081 tccttatcgt acctgcaacc cagtatatcc attatctcgt ttgcaatttc ccttctgcgc
     1141 ctgtagattt cgttgttctt ttcaaaccag tcgtctcctc ttttgagagc ttctgcagca
     1201 gcaagctgca ggggccggaa cattcctgaa tccatattgc ttttgacttt cagtactgta
     1261 ctgatataat ctctgtcacc tgccaccaat ccaactctcc atcctgccat attatgagat
     1321 ttgctgagtg aattaagttc aagtgcatat tcctttgcac cgtcaactga aagaatgctg
     1381 atgtgttcac tgttaagaat gaagctgtat ggattatcgt tacatatcag aattccatgt
     1441 tttgcagcaa attttatcag tcgttcaaaa agtattgcag atgcttttgt gcctgttggc
     1501 atatgagggt agttgaccca cataagtttc actttactta ggctggtgct ttcaatcaag
     1561 ttaatatcgg gcatccagtt attctttgcc agcaggtcgt atgtccttac aacaccaccc
     1621 acaagtctgg ttccggatgt atatgaaggg tagccagggt cgggaaccag cacctcatcg
     1681 cctggattaa cgaatgccat cgagatatgc attatgcctt ccttgcttcc gatgagcggt
     1741 aggatctcat tttccggatt gagtgtgacc ccaaaatatt tctggtacca gtcagacata
     1801 gcttttctca gttctggcag gccattatag ctctggtacc cgtgtactcc gggaagggct
     1861 gcctcttcag aaagcttctt aatagcttca ggaggaggag ggaggtcagg gcttccgatg
     1921 ccgaggttaa ttacatcaat gcctgcccgg cgcatctggt caatctgcct gagcttggcg
     1981 gagaaatagt attcccccac actgtttgtc cgttctgccg gtttaacttt catatatttt
     2041 gtcgtctttc agatatattc cgagtatctc catatcatga gtgtcgtctt caagaaggct
     2101 gtaaatcagt tccatagtag tatcttccgg aatctccaga tcgaggtaaa acatatattc
     2161 ccatccttta ccgttaagcc ttggtaccga ttgtatcttt gtaaggttaa tatttagttc
     2221 tgcaagcttt atcagtacat ttgccaggct tccaggccgg tggttcaacg aaaaacatat
     2281 tgaagcttta ttaccagttc tcaacccttt gcttcgacta cctataacaa ggaaacgtgt
     2341 gtaattctcg cggtaggtct caattccctc ggctaatatt tccagaccat acatctcagc
     2401 tgccactgac gaggctattg cggctattcc catgagcctc ttttcgctta ttattcttgc
     2461 actgccggct gtgtcttcgg tttctacaag ggttatttca ggatgctgat taaggaaagt
     2521 catgcactgt gagaaagcta tgggatgaga ccttatttcc tttatgtcgg ccagggtttg
     2581 cccgggtagt gccataaggt tttgttttat cctcagatta tgttcaccga gtattttcaa
     2641 ccccgactcc ctgataagtg tatagttata gagtatgctc ccggctcttg cattttcgat
     2701 agccattact gcaaagtcag ctcgtccggt ttttacttct gcaagagtct gatcgaaagt
     2761 ggcacaggga acaatatcaa tattgttgcc accgaagtat tgttttgcag ccacttcgtg
     2821 aaaacttcct gtttctccct gaatagatac tctaatatgt ccgttcatct ttatcatacc
     2881 ctgttaaact cgcaagggcc cctgcctgag ggacccttgc gcctttatct ttattatgta
     2941 agctacacac aaaaatccct cattctattc cctgccaaaa aagaaataga aatagaagaa
     3001 ggaaaagaaa taatatttat gtatagcgtt cattttctta aaaacgtcta caaaaatagt
     3061 ttttaatttt aaaaaaacaa acatcgggaa aaattatttt atttcatatc cgccgccagg
     3121 ttcccggcat ccggcagcga agggtagaag ccggttacaa tggggaaggc accagttcat
     3181 ttattcctgt gtcttttcat cagcacttcc tctatatctt cgagtttata tccttttgcc
     3241 ctgagcagaa ccaggaagtg gtacatcagg tcggcagctt cgttgcagaa tgactcatga
     3301 gaatcttctt ttgttgcctc tataaccagt tcaacagctt cctcgccagt tttctgtgca
     3361 attttgttga tgccttccct gtagagttta tttgtgtatg agttttctgc gttttcgtaa
     3421 atcctctggt tgattatctc ctcaagcctg tacataaatc cctttgccgg tgcatttcca
     3481 aagcacgatg tagtgcccag atgacatact ggtccggcgg gttttacctt tatcagcagt
     3541 gcatcattgt cacaatcggg caaaatttcc tccacataaa gaaaattgcc tgatgtctca
     3601 ccttttgtcc actgcgcttt cctggtacgg ctgtaaaaac taacacgtcc ttcattaatt
     3661 gttctgttga gggcttcctc atccatgaat ccaaccatca gaacctgaag ggtctgtttg
     3721 tcctgaatga ctgcaggcac aaggcctgac attttgctga aatcgatttt gttcatcatc
     3781 ttaccggaat attaaattgt gaaagatatt ttttcagttc gtagattttt atttctccgt
     3841 aatggaatat gctggcggca agggctgcgc tgcagcctgt gtgaagaaag acatctcgta
     3901 tatcttcttt actgcctgca ccgcccgatg caattacagg tatgttgaca ctttcgacta
     3961 tctttttagt tataccgatc gaaaagcccg accttgtccc gtcagcagtc attgatgtga
     4021 gaagtatctc accagcacct gcatcttcag ctcgctttgc ccattcaacc gtatcaatac
     4081 ctgttggagt acgtccgcca tttataaata ccttccatgt gttttctgca aagcctgtgt
     4141 caattgctac caccacacac tgactaccaa aacggtttgc tatctcagtg attagtccag
     4201 ggttttttac tgctgccgta ttgatgctta tcttatcggc tcctgcatta ataatagctt
     4261 ccacatcttt aagcgaattg atgccaccgc ccacagtgaa tgggatgttt attgccctgg
     4321 caacattttc aaccagcgaa acaagcgttt tacgcctttc aattgtggct gttatgtcga
     4381 gaaaaacaag ctcatcggct ccttctgcaa catacctgct ggcaagctcc acagcgtcac
     4441 ccgcatcctt tatattgaca aaatttactc ctttaaccgt cctcccatcc ttaacgtcga
     4501 ggcatggtat aattcttttt ttcagcataa ctccgcgaga tctttcagtt gtatgtaacc
     4561 ctcatatatt gctttaccca gtattgcacc ttcacaaccg gtttctgata acctgtaaag
     4621 gtcgttaacc gacctgattc ctccacttgc aattaggttg attttacatt ttgacaaaat
     4681 atctttatat agctctattg caggaccttc aagcatgccg tccctttcaa tgtcggtgca
     4741 taccacgtat gaaactcctt cgagactgta tctgctgata aaatcaatga tatcctcatc
     4801 tttttcagat gtccagcctt ccacacatac tttacggttg cgtgtgtcgg cgcttagaat
     4861 tatccgctca gtaccgtgtt ttttcagcca ttccagaaac atttctcggt tcaaaacagc
     4921 taaacttccg cagttaacct gcgaagcacc acactcaaag gctattctga catcatcatc
     4981 cgttcttatg cctcctccaa aatcgatgat gagcgatgtg gtcacagcaa tcttttcaag
     5041 taccctgtaa tttactaccc tgccttcccg ggcaccgtca agatcgacaa aatgaagata
     5101 ctccagtccg gcatcctcaa acatttttgc agtgtcaaca gggtcgccgc ggtactcttt
     5161 ctttgaagag aaatcgccgc gcgtcagcct tacacacttt cctcctatga tgtctattgc
     5221 aggtattatt ctcataacga aagaaaattt cttaatactt tttctccttt atctcccgac
     5281 ttttcgggat gaaactgcgt tgcataaaaa ttatccttct gcattgctgc actgaaggga
     5341 agaatatatt cacataccgc aacagttgac tctcctgttt cagcataata actatgaaca
     5401 tagtacatgt cgtcagattc tttcagtcca tcgaaaagtt ttccgtaaat acttatgaaa
     5461 ttattccatc ccatatgcgg taccacctgc agaggaggaa atttcctgac cttcgagtta
     5521 aatattccca gacattcagt gttatcttct tccgaagaga tgcacatcag ttgtagtccg
     5581 agacatatgc caagtacagg ttgtttcaga gaaagtataa ccctgtcgag tccgcgctct
     5641 ttcaggtatt tcattgcctg gccggcatga ccaacaccgg gtataatcac tttatcagct
     5701 ctgacgagct cttctttact gtcggtgata attgcttcat gtccggctct cgccagggca
     5761 ttaatcaccg attttatgtt tccagcgtta tatttcagta ttgcaatcat agttttcctt
     5821 ttgtgctcgg tatatcgcct cttcctgttt tatttactgc catgccaagt gcttttccaa
     5881 aggctttgaa gatggcttca gccttatggt gttcattgct gccctcagcc ttcacattga
     5941 tgttgcaacc ggcattgtcg gcaaaggttt tgaaaaaatg aaaaaccatt tctgacggca
     6001 tgtcacctat tttctccctt gtgaatgtga catcccatac cagccatggc ctgccgctca
     6061 ggtcaattgc aacattcgca agcgaatcat ccatcggcac tgtaaaacca aacctttcaa
     6121 taccctttct gctagccagt gccctcgcaa cagctctgcc cagcgcaatg gcactgtctt
     6181 caattgtatg atgctcatca acatcgaggt ctccttcagc cttaagttta atgtcgatat
     6241 tgccgtgtct cgcaatctgg ctgagcatgt gattgaaaaa tccgattcct gtgtcagctt
     6301 caattttacc tgttccgtcg aggttaactt cagccgttat cttcgtttca cctgttatgt
     6361 attcttcaat tgcctttcga ttaagacctg tgatataccg gaatatttca tcccagtcgg
     6421 atgtgcatag tgatgcataa tccgactttc tgcctccgaa aagtatggct ttgcagccaa
     6481 ggttttttgc cagctctaca tctgtcagcc tgtcgccaat tacatacgac gactgcaaat
     6541 ctattccctg tgaaagatat tttgtcagca gtgcagttcc gggttttctt g
//
LOCUS       DAOSNB010000141         6571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11808_length_6571_cov_5.403008, whole genome shotgun sequence.
ACCESSION   DAOSNB010000141 DAOSNB010000000
VERSION     DAOSNB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11808_length_6571_cov_5.403008"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="PLG42_06715"
     CDS             complement(<1..1868)
                     /locus_tag="PLG42_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU30936.1"
                     /translation="MLTTLVLWRVLCIFVSLEISLEASTCFQTFSENPQKVFIEKIFV
                     GNDSIVFASGETSGTEKLIESKIILGRRNNNISFQLSEPGEGHFSFMLENFDKEWTRW
                     QKSGVKDYTNLPAGKYLFRARYMEPGMSVIMSVPIEFKVVPAWYSSFTAISFYILLFC
                     LAAWAVYEQMKYRFARTQYMLEQIINARTEELIIEKEKSESLLANVLPKSTADELMAK
                     GKATKAKYNFVTVLFSDIQGFTKIAEETNPEVLIDELDKFFFYFDSVVEKYGIEKIKT
                     IGDAYMCAGGIPEKNRANPIEVILAALEMQEYMEKLKSSSEFEGMKLWDIRIGIHTGT
                     VVAGVVGQKKLSYDIWGDTVNTASRMESSGEPGKINISGTTYEFVKDFFICKYRGKMP
                     VKYKGEIDMYFVEGIREELRDENNRPNRKFITKMKKLRLSDVEEYILKYFDDEAPPNL
                     YFHNSSHARNVCNQVDLLATAEQIDDESYIPLKLAAIFLYTGYISDYNKPMEAAISLI
                     EEVLPKYSLLNEDIESTKKLIMNSYNEDTETLADFILHDAKYDWLGRVDYIKLIDKLF
                     REESEYGKVISREEWIQNQRKILVEHEFLTATARLLRSVTVEEQVSLLQDFGKELK"
     gene            1970..2575
                     /locus_tag="PLG42_06720"
     CDS             1970..2575
                     /locus_tag="PLG42_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOU30937.1"
                     /translation="MYMRLVFATNNKHKIREISELLGDNIELLSLEDVNIHCEIPEDY
                     NTLEENAMAKARYIHKITGMDVFADDTGLEIDALNGAPGVFSARFAGEEKDFEKNIDK
                     VLELMKDAENRKAAFRTVIALIKNEREYLFEGKVNGTILRERRGTGGFGYDPIFLPDG
                     YTQTFAEMTLNQKNMISHRAIAFRKLKSFLLQNNSCDNKNN"
     gene            2593..4875
                     /locus_tag="PLG42_06725"
     CDS             2593..4875
                     /locus_tag="PLG42_06725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU30938.1"
                     /translation="MRIFSSLPVLLLFTVSIKAQGPVGSWKDHLSYCSALVVAIGPDE
                     VYASTGSSLLIFNKKYSELRKMSKVQGLTETGISTIGWSEEREALIIAYQSTNIDIVS
                     GNNIFNVPEIKKKYFPGKKEIYKIRTRGKYAYLAGSLGIIVLDIGKKEIYDTWKPGEE
                     GTEVYDVAFDGTTIYAATNTGLYYSDLTIAGLAYSGNWFRINSFPNPLASFNTVGFLN
                     KKVYVNLSSRSGTDTIFCFDNSHNVTIFSLQSGIKNFSIETYSEGFIVASGSMVRIYN
                     TDGLLTSVISSYNSGSPDIAHAMAEGEDIWIADRSNGLIKYSISGNFTKLSLPGPYTN
                     NVLFIVNASNKTYIAGGALDNAWNNQWRPLQIFIHENNTWQWQLSNDLHDPLRILPDP
                     NNPDHFWVTTWGAGLLEYEKNTLVKKYDYTNSPLQTIIPGKPYTRICGIAMDRDRNIW
                     ITQTGVPGSIKVLKANNTWTQFPVTIDAPTIGDIIITSTGQKWIVLPRGYGLFVMDDN
                     GTIDNFTDDRYKQMLIRDNDNKIISNVYSIAEDLEGNIWVGTDQGPAVYFNPSRIFDE
                     DISAIRIKIPRNDGSGLADYMLGTEIISSISIDGANRKWLGTYNSGAYLLSADGTTKL
                     INYNEENSPLFSNMVVSIAIDGKTGEIWFGTSKGVASVRGEATTGAEKFSSIYAFPNP
                     VRENYEGVLTITGLMRNTSVKITDVSGNLVYKTISQGGQATWDLKTYTGERVSTGVYI
                     ILCASEDGEQTAVTKVLVIK"
     gene            complement(4884..6284)
                     /locus_tag="PLG42_06730"
     CDS             complement(4884..6284)
                     /locus_tag="PLG42_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOU30939.1"
                     /translation="MALKDNQELIRRIAKLAKQNQELEEHIKKLQKENEKLARELEKF
                     KALSEKLSPELLKEEGEKRERSLKFDMATILFADIHGFSKLVEEIDSTVLMDELDEIL
                     FEFDRIAENYKIEKIKTIGDTYMCAGGIPVKNITNPVDVVMAAIAMRNHLQEIENKKR
                     GPEKRIWDLKIGIHTGPVTATVTGKKKVSYDIKGDTVNTASRIQAVSEKGMILISVMT
                     YELVKEFFDCIYFGKLPVKYKGDLQIFEVKGLKPEFSVNGDGKTPNDAFRIKFGLIQF
                     TDIQEIVLDKLEKELPSYLYYHNVKHTVDVVTEVELIGWAEGCTDEEILLLKTAALFH
                     DAGHTIGYDNHEYYSTQIAREMLPKYNYTPEQIDRICEIIMATKLPPRPKNLLEEIIC
                     DSDLDYLGRSDFIPVSNTLFEELKAQNKINSLNEWNKMQVKFISNHQYFTKTARSLRE
                     VNKQQQIERIQSLITE"
BASE COUNT         2033 a         1346 c         1157 g         2035 t
ORIGIN      
        1 cattttaact ctttcccaaa atcctgaagt aatgaaacct gttcctcaac tgttacactt
       61 cttaataacc tggcagtcgc cgtaagaaac tcatgttcaa cgagtatctt tctctgattc
      121 tgaatccatt cctctctgct gataaccttc ccatattccg attcctccct gaacagcttg
      181 tcaatcagtt ttatgtaatc aacccttcct agccaatcat actttgcatc atgtaaaatg
      241 aaatccgcaa gggtttcggt atcctcatta taagagttca ttatgagctt tttggttgat
      301 tcaatgtctt cgttaagaag cgaatacttc ggcagaactt cttctatcaa actaatagcc
      361 gcttccatcg gcttattgta atcagatata tatccagtat aaagaaaaat agcagcaagt
      421 tttaatggaa tataactttc atcatcaatc tgttcggcgg ttgcaagaag gtctacctgg
      481 ttacagacat tccttgcatg cgacgaatta tgaaaataaa gattaggggg agcttcatca
      541 tcaaaatatt tcaaaatata ttcctctaca tcacttaagc gaagtttttt catctttgtg
      601 ataaacttcc tgtttggcct gttgttctca tctcgaagtt cttccctgat gccttcgaca
      661 aagtacatat ctatctcgcc cttgtactta acaggcattt tgccacggta tttacagatg
      721 aaaaaatcct taacaaattc gtaggtggtt ccagaaatat ttatttttcc aggttcacct
      781 gatgattcca tcctgcttgc agtatttaca gtatctcccc atatatcgta agagagtttc
      841 ttctgcccta caactccggc tacaactgtt cccgtatgaa tacctatccg tatatcccat
      901 aacttcatgc cctcaaattc cgatgaagac ttaagcttct ccatgtactc ctgcatttca
      961 agagcagcga gtattacttc aatgggattt gcgcggtttt tttctggtat gccaccggca
     1021 cacatgtaag catctccgat tgtcttaatc ttttcaatac catacttttc aaccaccgaa
     1081 tcaaaataaa agaaaaactt atccagttca tcaataagaa cctccggatt tgtttcttca
     1141 gctatcttgg taaatccctg tatatctgaa aataaaactg taacaaaatt atattttgct
     1201 tttgtcgctt taccttttgc catcaattca tcagctgtac tcttgggcag aacattagca
     1261 agcagacttt ctgatttttc cttttcaatt atcagttctt ccgttcttgc atttattatc
     1321 tgctcaagca tgtactgagt acgggcaaat ctgtatttca tctgctcata aacagcccag
     1381 gcggccagac agaacaacaa aatataaaat gatatagccg taaaagaact gtaccatgca
     1441 ggcactactt taaattcaat aggtaccgac attataaccg acatgccagg ctccatgtac
     1501 cgggcacgga atagatattt accggcagga agatttgtat aatcttttac ccccgatttt
     1561 tgccacctgg tccactcttt gtcaaaattt tcaagcatga aactgaaatg accctcccca
     1621 ggctctgaaa gctgaaacga aatattatta ttccgcctcc caagaattat tttgctttca
     1681 attagctttt ccgtcccgga tgtttctcca cttgcaaaga caatggaatc atttcctaca
     1741 aatatttttt ctataaaaac tttttgcggg ttttctgaaa aagtctgaaa acatgttgat
     1801 gcttccagac ttatttccag agagacaaaa atgcaaagca ctctccataa aaccagggtc
     1861 gttagcatca gtttcgcatc aaattattaa atgtaattca aaagtaacac ttattaaaga
     1921 aggcacaaag aggttagttt aattttaagt atttttggct ccatatccaa tctatatgag
     1981 actcgttttt gccaccaaca acaagcacaa aatcagagaa ataagtgaat tactgggaga
     2041 taatattgaa cttctcagcc tggaagatgt aaatattcac tgcgaaatac ccgaagatta
     2101 taatacactg gaagaaaatg caatggccaa agcaaggtac attcataaaa ttaccggtat
     2161 ggatgtattt gcagatgata cggggctgga aattgatgcc ctcaatggtg ctccgggtgt
     2221 cttttcggca agatttgcag gcgaagaaaa agattttgaa aaaaacatcg ataaagttct
     2281 tgaattgatg aaagacgcgg aaaatagaaa agcggctttc cggaccgtta tagcacttat
     2341 aaaaaacgag agggaatatc tctttgaagg taaagtaaat ggaacaattc tcagggaaag
     2401 aaggggaaca ggtggttttg gatacgaccc aatttttctg cctgatggtt acactcagac
     2461 atttgccgaa atgaccctta accaaaaaaa tatgatttcc cacagggcaa ttgctttcag
     2521 aaaacttaaa tcattcctgc tccaaaataa ttcctgcgac aataaaaata attagctgtc
     2581 agtttatcag gtatgagaat tttttcttca ctaccggttt tgctattatt tacagtttct
     2641 ataaaagccc agggacctgt aggttcgtgg aaggatcatc tttcatattg ctctgcactg
     2701 gtagtggcca taggacctga tgaggtttat gcgtcaaccg gaagttctct gctgatattc
     2761 aataaaaagt acagtgaact aagaaaaatg agtaaagtcc agggtcttac tgaaacagga
     2821 ataagcacaa ttggatggtc agaagaaaga gaagctctta ttatagctta tcaatcaacc
     2881 aatatcgata tcgtttcagg aaataatatt tttaacgttc ctgaaataaa gaaaaaatat
     2941 tttcctggca aaaaagaaat atataaaata agaacccgtg gaaaatatgc atatctggct
     3001 ggcagcctgg gcattattgt acttgatata ggtaagaagg aaatatacga cacatggaaa
     3061 ccgggagagg aaggtacaga agtttatgat gtagcttttg acggtacaac aatttatgca
     3121 gcaacaaata cagggcttta ttattcggat ttaacaatcg caggtctggc atattctggc
     3181 aactggttca gaattaattc attccctaac cctcttgctt cttttaatac cgttggcttc
     3241 ctgaacaaaa aggtttatgt aaatctgtct tcacgctcag gtacagacac aatcttttgt
     3301 tttgacaatt ctcataacgt aaccatattt tcgttacaat cgggcataaa aaatttttcc
     3361 atcgaaacat actctgaagg atttattgtt gcatcaggaa gtatggtgag aatttacaat
     3421 accgatggac ttcttacgtc agttatttct tcctacaact ccggttcacc tgatatagct
     3481 catgctatgg cagaaggaga agatatatgg atagccgaca gaagtaatgg tctgattaaa
     3541 tactctattt caggtaattt tacgaaactc tctctcccgg gtccatatac aaataatgta
     3601 ttatttatcg taaacgccag taataaaact tatattgcag gaggtgctct cgacaatgca
     3661 tggaataatc aatggagacc ccttcaaatc tttattcacg aaaacaatac atggcaatgg
     3721 caattatcaa atgatttaca cgatcctctc aggatactgc ccgaccccaa caatccagat
     3781 catttctggg ttacaacatg gggagctgga ttgctggaat acgagaaaaa cactcttgta
     3841 aaaaaatatg actatacaaa ttcacctctg cagacaataa ttccaggtaa accttataca
     3901 aggatatgcg gcattgcaat ggacagagat agaaatatat ggataacaca gactggagta
     3961 ccgggtagca tcaaagtact caaagcaaac aatacctgga cacaatttcc ggttacaatt
     4021 gatgctccta caatcggaga cattattata acttccacag gtcagaaatg gattgttcta
     4081 ccaagagggt atggactttt tgttatggat gataatggaa caatagataa ttttactgac
     4141 gacaggtata agcaaatgct tatacgggat aacgataata aaataatttc gaatgtttac
     4201 agcattgctg aagacctcga aggaaatatc tgggtaggaa ctgaccaggg tccggctgtt
     4261 tatttcaatc cttccagaat atttgatgaa gatatatctg caataagaat taaaatcccc
     4321 cgtaacgatg gttcgggcct tgcagattac atgcttggaa cagaaatcat aagctccata
     4381 tccattgacg gagcaaaccg taaatggctg ggtacttaca attccggtgc ttatctcctt
     4441 tcagccgacg gcacaacaaa actgatcaac tacaatgagg aaaacagtcc tcttttttca
     4501 aatatggtag taagcattgc cattgatggc aaaacaggtg aaatatggtt cggaacttca
     4561 aaaggcgttg cttctgtaag aggcgaagct actaccggtg cagaaaaatt tagcagcata
     4621 tatgctttcc ccaacccggt aagggaaaac tatgaagggg ttcttacaat tacaggactt
     4681 atgcgtaata catccgtaaa aataacagat gttagcggta atctggttta caaaactatc
     4741 tctcagggag gtcaggcaac ctgggatctg aaaacataca cgggagaaag agtctccact
     4801 ggagtctata ttattttatg tgcaagcgaa gatggagaac aaactgccgt aacaaaggtc
     4861 cttgtaatta aataaatcat taatcattct gttatgagac tctgaattct ttctatctgc
     4921 tgctgtttat taacttccct gagacttctg gctgtttttg tgaaatactg atggtttgaa
     4981 ataaacttaa cctgcatttt gttccattca tttagtgaat taatcttgtt ctgagccttc
     5041 aactcctcga aaagtgtatt ggagaccgga ataaaatcac ttctgcctaa ataatcaagg
     5101 tcggaatcac aaattatctc ttcaagcagg tttttgggcc ggggaggcag ttttgttgcc
     5161 attattattt cacatattct gtctatctgt tcgggagtat aattgtattt cggaagcatt
     5221 tccctggcaa tctgggtgct ataatactcg tgattatcat atcctattgt gtggcctgca
     5281 tcatggaata gggcagcagt tttaagaagc agaatctctt catcggtaca cccttctgcc
     5341 cagcctatga gttctacctc agtgactaca tccactgtgt gtttcacatt atgataatag
     5401 agatatgacg gcaattcctt ttccagcttg tcaagcacta tttcctgtat atctgtaaac
     5461 tggataagac caaattttat cctgaatgca tcattcgggg ttttaccatc cccgttaacc
     5521 gaaaattcag gttttaaccc tttaacttca aagatctgca ggtcgccttt gtattttacc
     5581 ggaagttttc cgaaataaat acaatcaaaa aactccttta ccagttcata agtcataacc
     5641 gatatcaaaa tcattccctt ttctgataca gcctgaatcc ggcttgctgt attcaccgta
     5701 tcacctttaa tgtcgtagct cacttttttc ttgcctgtca cagtagcagt gacaggacca
     5761 gtgtggatgc ctatcttaag atcccagatc ctcttctccg gcccgcgttt cttgttctca
     5821 atttcctgca ggtgattcct cattgcaatc gctgccataa ccacatcaac cggattggtg
     5881 atgtttttta ccggaatccc ccctgcacac atataggtat caccaatggt tttaattttt
     5941 tcaatcttgt aattctcagc tattctatca aattcaaaaa gtatttcatc taactcatcc
     6001 atcagaaccg tcgaatcaat ctcttctaca agttttgaaa acccatgtat atcagcaaaa
     6061 aggatagtcg ccatatcaaa tttgagcgac ctttccctct tttctccctc ttctttcagt
     6121 aattccggag ataatttttc ggaaagcgct ttaaacttct ccagctccct ggcaagtttt
     6181 tcattctctt tctgaagctt ctttatgtgc tcttcaagtt cctggttctg ttttgccagt
     6241 ttcgcaattc ttcttataag ttcctgatta tcttttaatg ccatcttctt tatcatgctt
     6301 gaatcgtaaa tatacaaaat ttattattag tgcttttagt aagaattatc ccggtttatt
     6361 caggctataa tacggaataa atccataatt ttgaaaaaaa ttatatcccg gtgattgacc
     6421 gtacggctgg aatagtactt cataacatta aatattccga ttcaggaact ataactcact
     6481 tttttactga aaaatttgga cgactcccgg taatgataag gggtattggt aacagaaaaa
     6541 ccggtaaaaa taaaatttat cttcagccac t
//
LOCUS       DAOSNB010000142         6545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11869_length_6545_cov_3.753621, whole genome shotgun sequence.
ACCESSION   DAOSNB010000142 DAOSNB010000000
VERSION     DAOSNB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11869_length_6545_cov_3.753621"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="PLG42_06735"
     CDS             complement(<1..1705)
                     /locus_tag="PLG42_06735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOU30940.1"
                     /translation="MSRLFLFSIISAALLTGCCIKEQEPVDLVNPYIGNISHLLVPTY
                     PTVHRPNSMLRFYPDRADYTAYRIPGFPLNIISHRSGKIFGITPFTGNPGSVPVNISY
                     IYDNEKITPYYYKVTLTEPDIKVEFTPEEKAGFFRIGFPGGSDAGIILRTTGQGELSL
                     NDDTVIGYDTFNGVKTWLWLEFDREPNSVSARRNDTLLAVNSIAGNRVALYASFGREQ
                     KPSEIKVRYGISYISAEQAKSNLYKSSDRWNFESIVKEGREIWNNTLSKIETEGGTHN
                     QQVVFYTSLYRCFERMIDITEDGWYYSPWTGKPEPVGDTAYYTDDWVWDTHLALHPLQ
                     VILNPSQQDEKINSYIRSARQSGWMPTFPVVTGDNHAMNGNHFAAVVLDAWNKGIRGF
                     SLEEAYNQLKKTIMEETKLPWVKGPATVLDKFYDEKGWFPGLKKGEAETVPEVHSFEK
                     RQSVAVTLASSYDDWCMAQMAKALGKEDDYEFFRKRSLNYRNLFNTATGFYHPKDSEG
                     NWIEPFDYRFDGGLGARDYYDENNGWTYIWQPYHAFDDLINMMGGPEKFDEKLDLLFT
                     VP"
     gene            1807..3162
                     /locus_tag="PLG42_06740"
     CDS             1807..3162
                     /locus_tag="PLG42_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOU30941.1"
                     /translation="MKKSIILFLTICALVAIVSCRHKEIPVTPETALERYLYNGDRTF
                     SWEIKENFEEGNMKVAILKLTSQKWREYTWVHQLTVVTPDEVKHNCALLFITGGSLKD
                     GEPRWKGRNDNLLGMLSAIAQRNKAVIAVISQVPNQSLYDTLTEDALISFTLHNYKQD
                     KDYSWPLLFPMVKSAVRAMDAVTDFCKKEHGAEINEFVLSGASKRGWTTWLTGSQDKR
                     VVAIAPMVIDVLNMPVNIAYQKEVWGDYSAEIEDYVRLGIAQDLSTSEGQELVAMIDP
                     YSYRDKLTMPKMIFIGTNDPYWPVDAVKNYINEIPGENYIHYVPNAGHDLGDGRQAIN
                     ALNAFFGTVINHLPHPSCEWEVKYDTLFAELTVKASQGLKSARLWMCDSPDRDFRNDI
                     FVVKNIEYQDISNIKTRVYYPEAGFRAFYIDLEYPSPSGGEYTKSTRMFVCSNRELFL
                     N"
     gene            complement(3205..5025)
                     /locus_tag="PLG42_06745"
     CDS             complement(3205..5025)
                     /locus_tag="PLG42_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU30942.1"
                     /translation="MNTIRILFKYISPYRWQAIKNVLFNIISAFFALFTYTLVGPFLN
                     LLFKGVKAAPEPGEFVFSLQWLLAFGRYKFYSYVTEHGEAQTILLVCVIVIIASLLKN
                     GFIFLANNNMAYIRAGTVRDLRKKLYDKVLRLPLSYFSDARKGDVMTRISNDVQEIEL
                     SVMSSLTMMFRDPLYIIIFVVYLMVTSVELTLFALIMMPVSGWLIGKASKTLKSSSFA
                     GQQSLGRLLSQVEETLSGLRIIKGFNAEKKVTSQFTDSNERYTKIFRRVTRKAYLASP
                     LSEFLATIVIMILMYFGARLAISGVGNMTPDKLITFMVVFSQIIPPAKNITTGYFMIQ
                     KGLASIERLDQLLNAEEKIKEKENALPVKSFNKAIEFQNVWFSYGTEPVLKDINLRIE
                     KGQTVAIVGKSGSGKSTLVDLIPRFMDVDSGRILLDGIDIRDLKIKDLRNLMGIVSQH
                     PILFNTSFAENIAFGMESFSMEDVINAARIANAHDFIMETENGYETTVGEGGCKLSGG
                     QRQRISIARAIMANPPILILDEATSSLDTESERYVQEAIVRLMKNRTSIVIAHRLSTI
                     QNADVIIVLDSGRIVETGTHKELMGKDNGFYKRLHSYQAI"
     gene            complement(5039..6382)
                     /gene="purB"
                     /locus_tag="PLG42_06750"
     CDS             complement(5039..6382)
                     /gene="purB"
                     /locus_tag="PLG42_06750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOU30943.1"
                     /translation="MNLDSLMAISPVDGRYRNKADELAEYFSEFALMKYRTMVEIEYF
                     IALCGIPLPQLKDFDAERFDLLRSFYREFTVEDARKIKEYEKITNHDVKALEYFLKEK
                     FTNSGLDKWKEFIHFGLTSQDINNTALPLSIRDTMAYVYTPLLFEIREALADMSERWK
                     NIPMLAHTHGQPASPTRVGKEFAVFVVRLDEQIKYFSQIPYSAKFGGATGNMNAHKAA
                     YPDIDWNAFAENFVNGVLGLKRSFPTTQIEHYDNLAALFDNMKRINVILTDLARDMWM
                     YISMDYFGQKTKKGEVGSSTMPHKINPIDFENAEGNFGIANALLIHMAEKLPVSRLQR
                     DLTDSTVTRNIGVAFAHTVIAFGSLLKGLDKLVINETKIREDLENNWVVVAEAIQTIL
                     RREGYDKPYEMLAELTRKEEGVTRETIMNFIDSLDISEEVRKELKAITPFNYTGI"
BASE COUNT         1835 a         1395 c         1341 g         1974 t
ORIGIN      
        1 atggcactgt gaagaggagg tcaagcttct catcgaactt ctcaggtcct cccatcatat
       61 ttattaagtc gtcgaaagca tggtacggtt gccagatgta cgtccagcca ttgttttcgt
      121 cgtaataatc acgggcacca agtccaccgt cgaacctgta atcaaaaggt tctatccagt
      181 tgccttcact gtctttcgga tgataaaaac ctgttgccgt gttgaagaga tttctgtagt
      241 tcagacttct ttttctgaaa aattcataat catcttcctt accaagtgcc ttcgccatct
      301 gcgccatgca ccagtcgtcg tacgacgatg caagcgtaac agctaccgat tgccgcttct
      361 caaatgaatg aacttcaggt actgtttcag cttcaccctt cttcaacccc gggaaccacc
      421 ccttctcatc gtaaaactta tccagcacag tggcaggtcc cttaacccat ggcagctttg
      481 tttcctccat tatagtcttt ttcagttggt tataagcctc ttccagactg aatcccctga
      541 tacccttgtt ccacgcgtcg agcacaacgg ccgcaaaatg atttccgttc atggcatgat
      601 tatctccggt aaccacagga aatgtaggca tccaaccgct ttgtcttgca ctgcggatat
      661 aggaattgat cttttcatcc tgttgtgaag gattgagaat aacctgtagc ggatgaagag
      721 ccagatgggt atcccatacc cagtcgtcgg tataatatgc cgtatctcca acaggttcag
      781 gtttgccagt ccagggactg taataccagc catcctcggt gatatctatc atcctttcaa
      841 aacagcggta aagagaggtg taaaaaacaa cctgctggtt atgagtacca ccctctgtct
      901 caatttttga aagagtgttg ttccagattt cccggccctc cttcactatt gattcaaagt
      961 tccatctgtc gctgcttttg taaagattac tttttgcctg ctcagcactg atatatgaaa
     1021 ttccgtatct gactttgatt tcagaaggtt tttgctccct gccgaaagaa gcatataatg
     1081 ccaccctgtt gcctgctatt gaattcacag ccagcaaagt atcgttccgt ctcgctgaaa
     1141 cactattggg ctctctatcg aactccagcc agagccacgt ttttacacca ttaaatgtat
     1201 cgtaaccaat gacggtatca tcgttaagcg acaattcgcc ctgtcctgtg gtgcgaagga
     1261 taatcccggc atcgctccct ccagggaaac caattctgaa gaatcccgct ttttcttccg
     1321 gagtaaattc aactttaatg tcaggttcag taagagttac tttataatag taaggtgtaa
     1381 tcttctcatt atcatagata tatgaaatat ttaccggaac actaccggga tttccggtaa
     1441 aaggtgttat tccgaatatt ttaccagagc ggtgcgaaat aatgttcagt ggaaatcctg
     1501 gtatcctgta tgcagtataa tctgccctgt cgggataaaa cctgagcata ctgttaggcc
     1561 tgtgaactgt aggatatgtc ggaacaagaa gatggctgat attcccgatg taaggattaa
     1621 caaggtcaac aggttcctgt tcttttatgc agcaaccggt caaaagagct gctgaaataa
     1681 ttgaaaaaag aaaaagtctt gacatggaca tgtctgattt tatttaattg ttcttaatat
     1741 cttctctaaa aaagttgttc gttttaaaat taaaatttta tttagcataa tagaaaaaat
     1801 cagcatatga aaaaatcaat aattcttttc ctgacaatat gcgcacttgt agccatagtg
     1861 tcatgcagac ataaggagat acccgttacg ccggagacag ctctggaaag gtatttgtat
     1921 aatggagaca ggacattctc ctgggagata aaggaaaatt tcgaagaggg caatatgaag
     1981 gttgctattc tgaaactaac atcgcagaaa tggcgtgaat acacatgggt tcatcagctt
     2041 actgttgtta cgcctgatga agtaaagcat aactgtgcat tactttttat aaccggtgga
     2101 agtttgaaag acggtgaacc caggtggaaa ggcaggaatg acaatttact tgggatgctt
     2161 tctgcaattg cacagaggaa caaagctgtt attgcggtta tttcccaggt tcctaatcag
     2221 tcgctttatg atactcttac agaggatgca ttaatctcct ttactctgca taattacaaa
     2281 caggataaag attattcctg gccgttgctg tttccaatgg ttaaaagtgc tgttagagca
     2341 atggatgcag ttacggattt ctgtaaaaag gagcatggag ccgaaatcaa tgagtttgtt
     2401 ctttcaggtg cttcaaaaag ggggtggacc acatggctta ccggctcgca ggataaaaga
     2461 gttgtagcaa tagcacccat ggtgatagat gttctcaata tgcctgttaa tattgcttat
     2521 caaaaagaag tgtggggaga ttacagcgcc gagatagaag attatgtgag gcttggcatt
     2581 gcacaggacc tttccacatc tgaaggacag gagcttgtgg cgatgattga tccttattcg
     2641 tacagggata aactcacgat gcccaagatg atatttatcg gaaccaacga tccctactgg
     2701 cctgttgatg cggtaaaaaa ttacataaac gaaatacccg gtgaaaacta cattcattat
     2761 gtacccaatg ccggacatga tctgggcgac ggccgtcagg caataaatgc actgaatgct
     2821 tttttcggaa cagtaataaa ccatttgcct catccatcgt gtgagtggga agttaagtat
     2881 gacacccttt ttgccgagct caccgttaag gcatctcaag gacttaaaag tgcaagatta
     2941 tggatgtgcg attctcccga cagggatttc aggaatgaca tctttgtggt aaaaaacatt
     3001 gaatatcagg atatttcaaa catcaagacc agggtttact atcctgaagc aggtttcagg
     3061 gctttttaca tcgatcttga atacccatct ccttcgggtg gagaatatac gaaaagcacc
     3121 aggatgtttg tatgcagtaa cagggagctt ttcctgaact gaatagtcat aaatacaatc
     3181 tacctatccg gttgaactgg taagtcagat agcctggtag ctgtgcaggc gtttgtagaa
     3241 gccgttatct tttcccataa gctctttatg agtgcctgtc tctactatcc gtccgctgtc
     3301 gagtacaata ataacatcgg cattctgaat tgttgatagt ctgtgggcta taactataga
     3361 ggtacggttt ttcatcagtc tgactattgc ttcctggacg tagcgttccg attcggtatc
     3421 gagggaagag gttgcctcgt cgaggatgag tattggagga ttagccatta ttgcccttgc
     3481 tatgctgata cgttgccgtt gaccgccgct aagcttgcat ccgccctcac caacagtagt
     3541 ctcatatccg ttttccgtct ccataataaa atcatgagca ttggcaatgc gtgcagcatt
     3601 tataacatct tccattgaaa aagactccat accgaatgca atattctctg caaaagaagt
     3661 attgaacaga atcggatgct ggcttactat gcccataaga ttcctcaggt ctttaatctt
     3721 taaatccctg atatcaattc cgtcgagaag tatcctgccg ctgtcaacat ccatgaaacg
     3781 tggtataaga tcaacaagtg ttgatttacc actacccgat ttaccaacaa ttgcaactgt
     3841 ttgtcctttt tcgattctca gatttatgtc ttttagaact ggctcggtgc cgtatgaaaa
     3901 ccatacattc tgaaattcaa ttgctttgtt gaacgatttg acgggcaggg cattctcctt
     3961 ttcctttatt ttttcctcgg cgttgagcag ctggtcgagt cgttcgatag atgccagtcc
     4021 tttctgaatc atgaaatagc cagttgttat attttttgca ggaggtatta tctgtgaaaa
     4081 gacgaccata aaagtgataa gtttgtcggg tgtcatattt cccactccgc taattgccag
     4141 ccgggcaccg aaatacataa gtatcattat aacaattgtt gcaaggaatt cgctgagcgg
     4201 tgaagcaaga tatgctttcc gggtaacccg ccggaatatt ttggtatatc tctcattgct
     4261 gtcggtaaac tgtgatgtaa cttttttctc agcgttaaag ccttttataa tcctcaatcc
     4321 cgatagtgtt tcttccacct gcgacaggag tcttccaagg ctctgctggc cggcaaatga
     4381 tgatgactta agtgtcttac ttgcttttcc aatcagccac ccgcttacag gcatcattat
     4441 cagagcaaag agtgtcaatt ctacacttgt caccattaag taaacaacga agattattat
     4501 gtagagtgga tccctgaaca tcattgtgag tgatgacatt acagatagct caatctcttg
     4561 cacatcgttg gaaattctgg tcattacatc tccttttcgt gcatcactga agtatgacag
     4621 gggtagcctc aatactttgt cataaagttt tttacggagg tcgcgaacag ttcctgccct
     4681 gatataagcc atattattat ttgcaaggaa aataaaaccg tttttaagca ggcttgcaat
     4741 aattactatc acacaaacaa gaagaatagt ctgagcctca ccgtgttcag ttacatagga
     4801 atagaattta tatctcccga aagccagaag ccactgcagg ctgaacacaa attccccggg
     4861 ctcaggagca gctttcacac ctttaaaaag aaggttcaga aatggtccaa caagtgtata
     4921 tgtgaagagt gcaaaaaatg cactgataat attgaacagg acatttttta tagcctgcca
     4981 cctgtatggt gagatatatt taaaaagtat ccttattgtg ttcattcccc ttcagaagtt
     5041 aaattcccgt ataattaaac ggagtaattg cctttaattc tttgcgtact tcctcgctga
     5101 tatccaggct atcgataaaa ttcattatag tttcgcgtgt aactccttct tcttttcgtg
     5161 taagttcggc aagcatttca tatggcttat cataaccttc ccttctaaga atggtttgta
     5221 ttgcttcggc aaccacaacc cagttatttt caaggtcttc tcttattttt gtctcattaa
     5281 tcaccagttt atcaagccct ttcagcaaag agccaaaagc aataactgta tgtgcaaaag
     5341 ccacgcctat atttctggtt actgttgaat ctgttaagtc gcgctggagt ctcgaaaccg
     5401 gaagcttttc tgccatatgg attaagagtg catttgcgat tccgaaattt ccttcggcat
     5461 tttcaaagtc tatcgggttt attttatgag gcatggtgga cgagcctacc tcgccttttt
     5521 ttgttttctg tccgaaatag tccattgaaa tatacatcca catgtctctt gcaaggtcgg
     5581 tgagtatgac attgattctt ttcatgttgt caaacagtgc tgcaagattg tcgtaatgct
     5641 caatctgcgt tgtagggaat gaccttttga ggccaagtac tccgttgaca aaattttctg
     5701 caaaagcatt ccagtcgatg tcagggtatg cggctttatg ggcgttcata ttgcctgttg
     5761 cccctccgaa ttttgcggaa taaggtatct gtgagaaata ttttatctgt tcatcgagcc
     5821 gtacaacgaa cacggcgaac tcttttccga ctcttgtcgg agaagccggc tggccatggg
     5881 tatgtgcaag catcggaatg tttttccatc tctccgacat atctgcaagt gcttctctga
     5941 tttcaaatag caaaggagta tagacatatg ccattgtatc tcttattgag agcggcagag
     6001 cagtgttatt aatatcctgc gaggtaagac caaaatggat aaactctttc catttatcta
     6061 gtccagaatt cgtaaatttc tcctttagaa aatactcaag agccttaacg tcgtggttgg
     6121 taatcttttc gtactcctta atctttctgg catcttcaac tgtaaattcc ctgtagaatg
     6181 aacgcagcaa atcgaacctc tcggcatcaa aatctttaag ctgaggaaga ggaatgccgc
     6241 acagggcaat aaaatactca atctcaacca ttgtccggta tttcataagg gcaaactccg
     6301 aaaaatattc tgcaagttca tccgctttgt tcctgtacct gccgtcaaca ggcgatatag
     6361 ccatcaggga atcaagattc atgtcctctg agtttaatta ttataatcac aaaactttca
     6421 aagcaaaagt agtattttag tcgcgaaaaa taatcacatg cccgttcaaa taagttgaaa
     6481 acgggtaaaa ttgtcaggtt aatggaaaag ataaggattt caatagttaa gtatgccaat
     6541 tccta
//
LOCUS       DAOSNB010000143         6527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_11906_length_6527_cov_4.911001, whole genome shotgun sequence.
ACCESSION   DAOSNB010000143 DAOSNB010000000
VERSION     DAOSNB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11906_length_6527_cov_4.911001"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..445
                     /locus_tag="PLG42_06755"
     CDS             65..445
                     /locus_tag="PLG42_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30944.1"
                     /translation="MRKILIFCISTLILFILAGSITAEAQQRPRTKGRSPEKSLFSNR
                     RNVKVVESRGVRKKKKEQERKEAKLKKEYQNFVKENKKRAYQIQSPEVKERMKQNKKE
                     IAARDRANRKKINADTRKAKKKYR"
     gene            579..1052
                     /gene="greA"
                     /locus_tag="PLG42_06760"
     CDS             579..1052
                     /gene="greA"
                     /locus_tag="PLG42_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOU30945.1"
                     /translation="MTDFYYITEEGLQKLREELDRLKNVERPQIAKMLAEATEKGDLT
                     ENAEYSAAKEAQAMLEMRIARLENQISRARIIDESKIDTSSVRILNKVKIRNKLNNSI
                     MEYQIVPESEANLKLGKIAVNSPIAQGLIGRKVGEVVEIKVPSGVIPFEIISISY"
     gene            1071..1475
                     /locus_tag="PLG42_06765"
     CDS             1071..1475
                     /locus_tag="PLG42_06765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOU30946.1"
                     /translation="MASIFSRIVSGEIPCYKVAEDDNYLAFLDINPLNTGHTLVIPKK
                     EIDYIFDIDDDTYAGLMLFAKRVARAIEKEVPCNRIGVAVVGLEVPHAHIHLIPINTM
                     DDMNFRRPKLKLTPEEFKSVADRIAKRFNEMT"
     gene            1556..2257
                     /locus_tag="PLG42_06770"
     CDS             1556..2257
                     /locus_tag="PLG42_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOU30947.1"
                     /translation="MSYISALVDDFISLLFPRVCYGCGEHLLRNENLICTQCYVSIPR
                     TNFHLDENNPVAQIFWGRCLIQKSAAFSFYEKGSRIRKIIHALKYNGIKELGNLMGRI
                     YAIQLKESGFFDGIDVIIPVPLHPSKQKKRGYNQSECICGGISEISGLPVNTSVLRRV
                     SSSATQTRRSRYDRWLNVEGIFDITDCDALRDKHVLLVDDVITTGATIESCVTALMKI
                     EGIKISIIALAWSSL"
     gene            complement(2258..2830)
                     /gene="ruvC"
                     /locus_tag="PLG42_06775"
     CDS             complement(2258..2830)
                     /gene="ruvC"
                     /locus_tag="PLG42_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOU30948.1"
                     /translation="MKKAVKEKIILGIDPGTNITGYGIISAGNSGPELITLGVIELKR
                     FHDHFLKIKHIFERTIGLIDEYHPDELAIESPFYGKNVQSMLKLGRAQGAAIAAALSR
                     SVPVFEYAPRKIKMSITGRGAASKEQVACMLMNILKFRETEIILDATDGLAAALCHYY
                     QNYYIGNIAPKTKEYKSWKDFIKQNPGRVK"
     gene            complement(2905..4209)
                     /locus_tag="PLG42_06780"
     CDS             complement(2905..4209)
                     /locus_tag="PLG42_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU30949.1"
                     /translation="MKRIWLAIVLISLLLSSCKKEEIIPVTNTSTNQEANEALYELMQ
                     DWYLWYQYLPQVNPANYKDPYELMNVLRYKNYDRWSFVADYDEFIASMQGTFVGHGIR
                     MGLDASKKVRIVMIYKNSPLYSKGVRRGWIIKQLNGVDLASVFLAGDAATYNQLIGPA
                     EEGVTNTFLFQTPAGKDSTIVTTKAKFTVNSVLADTIYDLSTGKTGYLAFNEFIEPSP
                     DELKAAFAKFKLNGVKDLILDMRYNTGGILSVAADLASFITTRSTTDTLVKCMYNDKK
                     YESNFFLYFKSKTESLNLSRLVVICTYETASASEVVINGLKPYLNVVLVGDTTNGKPT
                     GMNVWQYPAKSPKYVYAPVTFKMVNKDNYGDFFKGIPPDRYVSDDITHDFGDVNEYCL
                     KEALFYIENGSFSVKSTYPYGKVRYFTEKPVLTNNAYYLNWQ"
     gene            complement(4265..5155)
                     /locus_tag="PLG42_06785"
     CDS             complement(4265..5155)
                     /locus_tag="PLG42_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU30950.1"
                     /translation="MSPSRQSYLYAGLSVLFWSTIPTVFKIGLGELNVLTMLTIATLT
                     STFVLFVLILAERKLSLLGRTSINELLVSALLGFINPFIYYIILLKAYSLLPGQVAQP
                     LNMIWPIILVFLSVPLLKQKIPSKSFLALFISFAGVYIVSSQGHLTKPGSSDPIGVLL
                     ATGSSFFWALYFIFNLRDKRDEAVKLFTNFLFASVYLLIIMTLTGKWNAEVSLKGAGA
                     SVYAGIFEMGITFFFWLKALSLAETSDRVSNLVYIAPFLSMVFLHFIVKEPVYYTTPL
                     GLIFIITGIFIQNRNTHVAR"
     gene            complement(5182..6456)
                     /gene="serS"
                     /locus_tag="PLG42_06790"
     CDS             complement(5182..6456)
                     /gene="serS"
                     /locus_tag="PLG42_06790"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOU30951.1"
                     /translation="MLTLNLIREQKDFVVERLKIKNFDAASLVDKILELDAIRRDTQK
                     NYDAILADINRISKEIGELIRSGKKAEADAAKTKTTSLKEESRRLAERLDEIDKELRS
                     ELIKLPNLPHESVSPGHGAENNVKVREGGVIKELEETALPHWELIKKYDIIDFELGTK
                     LTGAGFPVYKGKGAKLERALINFFLDEGEKAGYKEVLPPIVVNEDSAFATGQLPDKEG
                     QMYLIGSDNYYLIPTAEVPVTNIFRDVILDAADLPIKTMAYTPCFRREAGSWGAHVRG
                     LNRLHQFDKVEIVQIAHPDHSYEALEEMVSHVEGLVKKLGLPYRIMKLCGGDMSFTSA
                     LTYDFEVWSGAQKRWLEVSSVSNFESFQANRLKCRFREKGGKQNRLVHTLNGSALALP
                     RIVAALLENNQTPSGIIVPEVLAKYTGFNIID"
BASE COUNT         1916 a         1289 c         1389 g         1933 t
ORIGIN      
        1 aacggaacat acttttatgt gactttgcaa atttaccttt aaactgttaa acatttataa
       61 tggtatgaga aaaattttga ttttctgtat aagcactctt attttgttca ttcttgccgg
      121 gtctataact gccgaagcac agcagcgtcc aaggacaaag gggagaagtc cggaaaaaag
      181 tttgttcagt aatagaagaa atgttaaagt ggtggaatcg agaggtgtga ggaaaaagaa
      241 aaaagaacag gagagaaaag aggcaaaact caaaaaagag taccagaatt ttgtaaagga
      301 aaacaaaaaa agggcatacc agatacaatc gcccgaggta aaagaacgga tgaaacagaa
      361 caagaaagag atagccgcaa gagatagagc taacaggaaa aagattaatg cggatacccg
      421 gaaggcaaaa aagaaatacc ggtaattata taaaacgttt atttgcaata aaaaagatct
      481 ttatttttgt tttttttaaa aatattttat atttgtattc atgaagagtt ccggaaaccg
      541 ccggaattct tcttcttttt taaattacac aagcagaaat gacggatttt tactacatta
      601 ctgaagaggg tttgcagaaa ctcagggaag agcttgatcg tctgaaaaat gttgagagac
      661 ctcagatagc taaaatgctt gctgaggcca cagaaaaggg agatcttact gaaaacgctg
      721 agtattctgc tgcaaaggaa gctcaggcta tgctcgaaat gaggatagca aggcttgaga
      781 accagatctc acgggcaagg attattgatg aatctaaaat tgacacttcg tcggtgagga
      841 ttctgaataa ggtgaaaatc aggaacaagc ttaataacag cataatggaa tatcagattg
      901 ttcctgaaag tgaggccaat cttaaactgg gtaaaattgc tgtgaactca cccatagccc
      961 agggacttat tggcagaaag gtaggagaag tggttgagat aaaagtgccc tcaggggtaa
     1021 tacctttcga aataatttca atttcatatt gattgataaa actccggatt atggcatcaa
     1081 ttttttcaag aatagtcagt ggagagattc cctgctacaa ggtggcagag gatgataatt
     1141 atcttgcatt tctcgatatt aatcctctaa ataccggtca tacactggta attccaaaga
     1201 aagagataga ctatatcttt gacattgatg atgacactta tgccggactg atgctttttg
     1261 caaagagagt cgcaagggca atagagaagg aagtgccatg caacaggatt ggcgttgcgg
     1321 tggtgggcct tgaagtgccc catgctcata tacacctgat tcctatcaac acaatggatg
     1381 atatgaactt ccgcaggcca aaactgaaat taacccctga agaattcaaa tctgtagccg
     1441 accgcattgc aaaaagattt aatgaaatga cctgaaaaaa caatatcttt tttccttcat
     1501 gcctgaatat tatttcaggc attttttatt tttgcaatgt aacacgtccc gctgcatgag
     1561 ttatattagt gctcttgttg atgattttat ttcccttctt tttccgagag tgtgttatgg
     1621 ctgcggtgaa caccttctca gaaacgaaaa ccttatttgc actcaatgtt atgtgtcaat
     1681 accccgaacc aattttcatc ttgatgaaaa taatccggtg gctcagatct tctggggccg
     1741 ctgtttgata cagaaatccg ctgcattttc cttttatgag aagggaagcc gtataagaaa
     1801 aataattcat gctctaaaat ataatggtat taaggaactc gggaacctta tgggcaggat
     1861 ttatgcaatc cagcttaaag agagtggttt tttcgatggg atagatgtta ttatccctgt
     1921 accgttgcat ccttcaaagc agaaaaaaag aggctataac cagagtgagt gcatctgcgg
     1981 gggaatatcc gaaatatcgg gtttaccagt aaatacttct gtacttagaa gagtttcttc
     2041 atcagcaact cagacccggc gttcgagata tgatcgctgg cttaatgtgg agggaatttt
     2101 tgatattaca gattgtgatg ctctcagaga taagcatgtt ctgctggttg atgatgtaat
     2161 tacaaccggt gccaccatag aatcttgcgt aacggctctt atgaagatag aaggtataaa
     2221 aataagcata atagcacttg catggtcgtc gctgtaatta ttttaccctt cccgggttct
     2281 gttttataaa atctttccag cttttgtatt cttttgtttt gggtgcgatg tttccgatat
     2341 aataattctg gtaatagtgg cataatgctg ctgccagtcc gtcggtggca tcgagaatta
     2401 tctctgtttc tctgaatttc aggatattca tgagcatgca ggctacctgt tcctttgaag
     2461 cagcacctct gcctgttatc gacattttga tttttctcgg agcatattca aaaaccggta
     2521 ccgagcgcga gagtgccgca gcaattgcag ctccttgagc ccttcccagc ttgagcatcg
     2581 actgtacgtt ctttccataa aaaggagact caatggcgag ctcatcagga tgatattcat
     2641 caatgagccc gattgttctt tcaaaaatgt gtttgatctt caagaaatga tcatggaatc
     2701 tctttaattc tattactcca agagttatca gttcaggacc ggaatttcct gcagaaataa
     2761 ttccatagcc tgtaatattt gtaccaggat cgatgccaag tatgattttc tcttttacag
     2821 cctttttcaa gattaatctc tgtttcttta ttttttattt acttaacctt tctgcctccg
     2881 ggacgacact ttgcactgca gccttcattg ccagttaaga tagtatgcat tgtttgtcaa
     2941 taccggcttt tcggtaaagt aacgcacctt accgtatgga taggtacttt ttacggaaaa
     3001 agatccgttt tcaatataaa agagtgcttc tttgaggcaa tattcgttta cgtcaccaaa
     3061 atcatgggtg atatcatccg aaacatatct gtcgggagga attcctttga agaaatcacc
     3121 atagttgtct ttattgacca ttttgaaagt gacaggtgca tagacatatt tagggctttt
     3181 cgcagggtat tgccatacat tcatacctgt aggtttgcca ttggttgtat cgcctacaag
     3241 aacgacattc agatagggtt taagtccgtt aataaccacc tcgctggccg atgcagtttc
     3301 gtatgtacat ataaccacaa ggcggctgag attcaatgat tctgtcttgc ttttgaaata
     3361 gagaaaaaag tttgactcgt attttttgtc gttatacata cactttacaa gtgtatctgt
     3421 agtagaacgt gttgtaataa aacttgcaag gtcagctgca acactgagta ttccgcccgt
     3481 gttgtatctc atatccagaa taaggtcttt aacgccgttg agcttaaact ttgcaaaagc
     3541 tgctttaagc tcatcgggtg aaggttcaat gaattcgtta aatgccagat atcctgtttt
     3601 acccgttgaa aggtcgtata ttgtatcagc cagaacggaa ttgactgtga atttggcttt
     3661 ggttgtcacg atggtggaat cttttccggc gggtgtttga aaaagaaaag tattggtaac
     3721 gccttcctcg gctgggccta tcagctggtt atatgttgca gcatctccag caagaaaaac
     3781 tgaagcaaga tccaccccgt tcaactgctt gattatccat ccacgcctca ctcctttaga
     3841 ataaagtggt gaattcttat atatcattac aattcttact tttttcgaag catcaagtcc
     3901 catgcgtatt ccgtgaccca caaaggttcc ctgcattgag gcaataaatt catcgtaatc
     3961 tgcaacaaaa ctccagcggt cgtaattttt gtacctgagg acgttcataa gttcgtaggg
     4021 atctttgtaa tttgccggat ttacctgagg aagatactgg taccacagat accaatcctg
     4081 catcagttca tacagagctt cattggcttc ctgattggta gaggtatttg ttactggtat
     4141 tatctcttcc ttcttgcacg aacttaataa caaggagatc aacactattg caagccagat
     4201 tcttttcata cttaaacatt ttgaaatatt tttcatcaat aataaattca ttgcagcatt
     4261 acaattaacg tgccacatgt gtattgcggt tttgaataaa aattcccgta ataataaata
     4321 tcaacccaag tggggttgta taataaacgg gttctttgac aataaagtgc aggaaaacca
     4381 ttgaaaggaa aggagcaata tatacaaggt tgcttaccct gtcgcttgtt tcggcaagac
     4441 tgagtgcttt gagccagaaa aaaaatgtga ttcccatctc aaaaatgcct gcataaactg
     4501 atgcgccggc acctttaaga ctcacttcgg cgttccattt tcctgtcagt gtcattatta
     4561 tcagaaggta aactgaagca aacagaaagt tagtaaataa tttgacagcc tcatcccgtt
     4621 tatctctcag gttgaagatg aaatatagtg cccagaagaa tgagcttccg gtggcaagta
     4681 acacaccgat ggggtcggag ctgccgggtt ttgtaagatg cccctgggat gatacaatat
     4741 agacaccggc aaaactgatg aatagtgcga gaaagctttt tgagggtatc ttctgtttca
     4801 acagcggcac agacaggaac accagtatta tcggccagat catattcagc ggctgggcta
     4861 cctgtcctgg caaaaggcta tatgccttaa gaagaataat gtagtagatg aacggattaa
     4921 taaatccgag aagtgccgat accagcagtt catttataga ggttctcccc agaagtgaaa
     4981 gttttctttc tgccagtatc aacacgaaaa gtacaaaggt ggaagtcagc gttgcaatag
     5041 taagcattgt aagaacattg agttcaccca gaccaatttt gaaaacagta gggatagttg
     5101 accagaacag aaccgaaagc ccggcgtaga gatatgattg tctggacggg ctcatttttc
     5161 agcaaggtag tttaatggtt atcagtcgat gatgttgaat ccggtatatt ttgcgagcac
     5221 ttcaggtact atgatgcctg agggggtctg gttgttttcg agcagggcag ctactattct
     5281 gggcagtgca agggcgcttc cgttaagggt atgcaccagc cggttttgtt tcccgccttt
     5341 ttccctgaac ctgcatttca gacggttggc ctgaaacgat tcgaagttgg agacggagct
     5401 cacttcaagc catcttttct gggcgcctga ccacacttca aaatcgtatg taagagctga
     5461 agtaaacgac atatctccac cgcagagctt cataatcctg tagggcaggc cgagcttttt
     5521 gaccagccct tccacgtgcg acaccatctc ttcgagagcc tcatatgaat ggtcgggatg
     5581 agcaatctgg acaatttcaa ccttgtcgaa ctgatggagc cggttgagcc ctctcacatg
     5641 tgcaccccac gagcctgctt cgcggcggaa acagggagta tatgccatgg tcttaatggg
     5701 caggtcggca gcatccagaa taacatccct gaaaatattt gtgacgggta cttctgcagt
     5761 ggggatcaga taataattgt cggaacctat tagatacatc tgcccctctt tatcgggtag
     5821 ctgacctgtt gcaaaggccg aatcctcatt gaccactata gggggtagga cctccttata
     5881 tccagctttt tcgccttcat cgagaaagaa attgataagt gccctctcga gcttggcgcc
     5941 tttaccttta taaacaggga aaccagcacc tgtaagtttg gttccaagtt caaaatctat
     6001 aatgtcatat ttctttatca gttcccagtg tgggagtgca gtctcttcaa gctcttttat
     6061 aacgccgcct tccctgacct tcacattgtt ttccgcacca tgtccgggag aaaccgattc
     6121 gtgaggcaga ttgggaagtt ttataagttc acttctcagc tctttatcaa tttcgtcaag
     6181 cctctcagcc agcctgcgtg attcttcctt cagtgaagtt gtctttgttt ttgcagcatc
     6241 cgcttcagct tttttaccgc tccgtattaa ctctcctatc tcttttgaga tcctgttaat
     6301 atctgcaaga attgcatcat agtttttttg agtatccctg cggatagcat cgagttcgag
     6361 aattttatca accagggacg ctgcatcaaa attcttgatc ttcagcctct caacaacaaa
     6421 atccttctgt tctcgtataa gatttaatgt aagcatagat ttattaaaaa taaaaaagca
     6481 ggcataaatc agcctgctgt aaaattatta aaaaagttgt atgaacg
//
LOCUS       DAOSNB010000144         6481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12005_length_6481_cov_3.910364, whole genome shotgun sequence.
ACCESSION   DAOSNB010000144 DAOSNB010000000
VERSION     DAOSNB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12005_length_6481_cov_3.910364"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLG42_06795"
     CDS             complement(<1..507)
                     /locus_tag="PLG42_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOU30952.1"
                     /translation="MLKRVAIFSSRVNERTVRVVNHLIEEFCRKNIRIYLYDKTGELR
                     KCHDDYSVEVIVDAAELKEKPELILSVGGDGTFLETMMLVKDLEVPIAGINTGRLGFL
                     ANISEEEACHSLEFIYNGDFEIVERSLLEITEPAGKFGGYDPVALNEVTVQKSGLSMI
                     TINVYVDDV"
     gene            complement(515..1180)
                     /locus_tag="PLG42_06800"
     CDS             complement(515..1180)
                     /locus_tag="PLG42_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOU30953.1"
                     /translation="MLAKDLISETVPALKTSDTGQTALNWMEVFRISHLPIVNNQDFL
                     GLISDADIYDLNQPDEPIGNHKLTLLKPYVTMEQHIFEVIALAAKLKLTVIPVLDENG
                     HYRGVITANDLIQHVAKFSSMDQPGGIIILEMAERDYSLTQIAHIAESNNLKILSMFI
                     TSSPDSIRLEVTLKLNSNDLTSAIRTYERYGYEVKKWVSTDDMLDRFYAERLDMLIKY
                     MNI"
     gene            1278..1994
                     /locus_tag="PLG42_06805"
     CDS             1278..1994
                     /locus_tag="PLG42_06805"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOU30954.1"
                     /translation="MTKLSVNINKIATLRNARGGNIPDVKLAAIKCQEFGADGITVHP
                     RPDERHIRYSDVYEIKPIITTEFNIEGYPSEKFMNLVLSVKPNQVTLVPDPPGAITSN
                     AGWDTKASFLFLKDIVSRLKDAGIRTSIFVDPISENIEYAAKTGTDRIELYTEPYARN
                     YPASPEKAIAPYIKAAETARALGLGINAGHDLNLDNLRYLSENIPYLAEVSIGHALIS
                     DALYYGLENTIQMYKRLLVE"
     gene            complement(2031..2759)
                     /locus_tag="PLG42_06810"
     CDS             complement(2031..2759)
                     /locus_tag="PLG42_06810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU30955.1"
                     /translation="MSMLENIKLENVLFLDIETVPAAASFDELTPLMKVLWDRKSAGF
                     RTPEQSAADVYQRAGIYAEFGKIICISVGLIKEREPFSFRLKSFFGDDEKTILEDFAS
                     MLRKFSAGRDIVLCAHNGKEFDYPYIARRMIVNSIPLPDILDNAGKKPWEVKLLDTME
                     LWKFGDHKNYTSLEVLAAILGIASPKEGIDGSMVADVYWKEHDLQRIVRYCEKDVLCV
                     AQILLRFMNEPPINQDKIESVTVI"
     gene            complement(2756..3379)
                     /gene="udk"
                     /locus_tag="PLG42_06815"
     CDS             complement(2756..3379)
                     /gene="udk"
                     /locus_tag="PLG42_06815"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOU30956.1"
                     /translation="MLIVGIAGGTGSGKTTVVNKIVNEFSNGEVVVLPQDAYYRDNSH
                     IPFEERQKINFDHPDSVEFPLLIEHIRKLKSGQKINMPVYSYITCLRSKETIEVAPAR
                     VIIVEGILILVDAELRQLFDIKVFVDADADDRLGRVIIRDIAERGRSVMQVLERYHET
                     VKPSHLQFIEPSKRYADIIIPGGGENNIGIGVLISMIRQHLQKEAIK"
     gene            3568..4569
                     /locus_tag="PLG42_06820"
     CDS             3568..4569
                     /locus_tag="PLG42_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOU30957.1"
                     /translation="MHELYPLKFEPILKEKVWGGNSLVLEYGKKADPSKLIGESWEIS
                     AVADHLSVVSNGFLAGNNIEEIIEVYMSDITGEAVYEKFGIEFPLLIKFIEASSDLSV
                     QVHPDDRIAKDKHNAYGKTEMWYILKALPGSKIYTGFKEPLTEEKLREAIYAGKLPEL
                     LNIEYPQPGDVFFTPAGRIHAIGGGITLVEIQQTSDITYRIFDWNRTGQDGKPRELHV
                     EYAIDAIDYNATGSALIRKEPVPDKTINLVTCDYFVTNIIHLTGVLRKDYNLIDSFVI
                     YICTEGKFSVHFGSGTETAVKGETLLVPAAITDVILEAQPEAVILEVFIKSQKINES"
     gene            4566..5339
                     /locus_tag="PLG42_06825"
     CDS             4566..5339
                     /locus_tag="PLG42_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30958.1"
                     /translation="MKKISFLILAFLSVVYFTGCLKKSQQKQAEKETQEEVSVPDTGY
                     TGIKQYYSGNFLAYEVEFKNGVRHGLMKTYYYPSGKLRQTFWYQNGMREDTAVWYYED
                     GRVFRKTPYKRDTMHGVQIQYYKSGRIRAKLEFENGLRKPYLEEFTSDGKKITDYPEV
                     VIKTEDNYGKNGTYKILLSLSKKNVKVNFYRGGYINGLFDPGKLVKINDSDYTGVLTL
                     KKSGVQGENYVEIIAEITTPLGNKLLVNKKIDLPYNDLR"
     gene            5379..5987
                     /gene="yihA"
                     /locus_tag="PLG42_06830"
     CDS             5379..5987
                     /gene="yihA"
                     /locus_tag="PLG42_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOU30959.1"
                     /translation="MVIRTVKFFSASSSLSKCPPASKPEFAFIGRSNVGKSSLINMLA
                     GNRKLAKISQTPGKTRTINHFLVNNEWFLVDLPGYGYSKMPYHERKKWLQETGNYILK
                     RETLVSLFVLIDVRIEPQETDLRFMEMLGVNKVPFAMVFTKSDKISRERVKRNIEHYN
                     RVMLEKWEELPPEFVSSAVTKAGKEEILNFIEESINNLKKSK"
     gene            6049..>6481
                     /gene="prs"
                     /locus_tag="PLG42_06835"
     CDS             6049..>6481
                     /gene="prs"
                     /locus_tag="PLG42_06835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU30960.1"
                     /translation="MFGKAPMKIFACRASRALAENIASELGVELGKSSVTVFSDGEFQ
                     PCYDESVRGCLVFIVQSTNPPAENLLELLLMIDAAKRASAYKVIAVIPYYGFARQDRK
                     DRPRVSIGSKLSADLLSAAGVDRIITMDLHADQIQGFFNVPV"
BASE COUNT         2031 a         1363 c         1238 g         1849 t
ORIGIN      
        1 aacatcatca acgtaaacat taatagttat catgctcagc cccgacttct gcactgttac
       61 ttcattgagt gctaccgggt cataccctcc gaatttacct gcgggttctg ttatttccag
      121 aagagatctt tcgacaattt caaaatcgcc attatatata aattccagcg aatgacacgc
      181 ttcttcttca gatatattgg caagaaaacc gagtcgccct gtatttattc ctgcaatagg
      241 tacttcaaga tctttaacca gcatcatggt ctcaagaaat gtcccgtctc cccccacact
      301 caatataagc tccggcttct ctttaagttc tgctgcatcc actattactt ccactgaata
      361 atcatcgtgg catttcctca attcacccgt tttgtcgtaa agataaattc tgatgttctt
      421 tctgcagaat tcttcaatca ggtgatttac cacccttaca gtccgttcat taacccttga
      481 actgaatata gctacccttt ttaacataga agcatcagat attcatatac tttatcagca
      541 tatcgagccg ctcagcataa aatctgtcaa gcatatcatc ggttgaaacc cattttttga
      601 cttcataccc gtatctctca taagtcctga tagccgaagt caggtcattg gaattgagct
      661 tgagagtaac ctcaagcctt attgagtcag gagatgacgt aataaacatg ctgagtatct
      721 taagattgtt actttcagct atatgtgcaa tctgggtcag tgaataatct ctttcagcca
      781 tctcaagtat aattatgcct cccggctgat ccatcgacga gaacttagca acatgctgaa
      841 taagatcatt tgcagtaata actcccctgt aatggccgtt ttcatcaaga actggaatca
      901 cggtaagttt gagcttcgca gcaagagcaa taacctcaaa aatatgctgc tccattgtta
      961 cataaggctt cagcagggta agcttgtgat tccctatggg ttcatcaggc tgatttaaat
     1021 catatatgtc tgcatcagaa ataaggccca gaaaatcctg gttgtttaca atgggaagat
     1081 gagaaatcct gaagacttcc atccagttca gggctgtctg ccctgtatct gaggttttta
     1141 aagcaggaac agtttctgat atgagatctt ttgcaagcat ctttaagttt taccatctaa
     1201 ttttctccta aaatagcgaa aaaaacgctt acttattata tttttgtcat ataattattt
     1261 caccttatgc ggcacttatg acaaaattaa gtgtaaatat caataaaata gctactcttc
     1321 gaaatgccag aggcggcaac attcctgatg taaaacttgc agcgataaag tgccaggagt
     1381 ttggtgccga tggaataaca gtccaccccc ggcctgatga gagacatata cgttactccg
     1441 atgtatatga aataaaaccc attatcacaa ctgaattcaa tatagaagga tacccttcag
     1501 agaaatttat gaatctggtt ctttcagtaa agcccaacca ggtgaccctt gtacccgatc
     1561 ctccaggtgc aataacatca aatgcagggt gggacacaaa agccagcttt ctgttcctta
     1621 aagatatagt ttcaagactg aaggatgcag gtatccggac ttccatcttc gtcgatccca
     1681 tttctgaaaa tattgaatat gcagctaaaa ccggaaccga caggattgaa ttgtatacag
     1741 aaccctatgc cagaaattac cctgcaagcc ctgaaaaggc aatcgcacct tatattaaag
     1801 cagctgaaac agccagagca ctgggattag gaatcaatgc aggccacgac cttaatctgg
     1861 ataatctaag atatctttca gaaaacatcc cgtatctcgc agaagtttct ataggccatg
     1921 ctctgatttc cgacgcactc tattacggac ttgaaaatac aattcagatg tataaacgcc
     1981 ttctggttga ataatttttc accctacctt gccagggtct tccttcctgc tcagattacg
     2041 gtaactgatt caatcttgtc ctgatttatc ggcggctcat tcataaacct aagcagtatc
     2101 tgcgcaacac ataagacatc tttttcacag tatctgacta tacgctgaag atcatgttct
     2161 ttccagtaaa cgtcggccac catgctgccg tcaattcctt ccttaggtga agcaatgcca
     2221 agaatggctg caagaacttc aagagaagta taattcttat ggtctccaaa tttccacagt
     2281 tccattgtat cgagcaattt aacctcccat ggcttcttgc ctgcattatc gagtatgtcg
     2341 ggtaggggta tactgttcac tatcatcctt cttgcaatat acgggtaatc aaactctttc
     2401 ccgttatgtg cacaaagcac tatgtccctg ccagcagaaa attttctgag cattgaggca
     2461 aaatcttcaa gtatggtctt ttcatcatca ccgaaaaatg atttaagcct gaaactgaaa
     2521 ggttctctct cttttatcag acctaccgaa atacatatta ttttgccaaa ttccgcatat
     2581 attcctgcac gctgatatac atcagccgca ctctgttcag gggttctgaa tccggcagac
     2641 ttcctgtccc agagcacctt catcaaagga gtcagctcat caaaggaagc agctgcagga
     2701 actgtttcga tatctagaaa cagaacattt tcaagtttta tattttcaag catactcatt
     2761 ttatggcttc tttctgtaaa tgttgacgta tcattgaaat caatactcct attcctatat
     2821 tgttctcacc acccccggga attatgatat ctgcatacct ttttgatggt tcaataaact
     2881 gcagatgcga tggcttgaca gtttcatgat acctctccag tacctgcatc accgaccttc
     2941 ccctctcagc aatatccctt attattaccc ttcccagcct gtcgtcagca tcagcatcga
     3001 caaacacctt aatgtcaaat aactgcctca gttcagcatc aacaagtata agaatgcctt
     3061 caacaataat tacccttgca ggtgcgacct caatggtctc cttcgacctc aggcatgtta
     3121 tatatgaata aacaggcata ttgatctttt gccccgactt caactttctt atatgctcaa
     3181 taagcaaagg gaactctaca gaatcgggat ggtcaaagtt tatcttctgc ctttcttcaa
     3241 acgggatgtg gctgttatcc ctgtaatatg catcctgcgg cagcacaaca acttcaccgt
     3301 ttgaaaactc gttaactatc ttgttgacca ctgtcgtttt tccagaacct gttcctcctg
     3361 caatcccaac aattagcata atttttctat ttttgtctga tatttcaccg atcaaaaata
     3421 aaaagaaaat aatgaaaagg tacaggtaaa tctgtcctct gtcaagaaga aggtccgaac
     3481 aggatctatt tatgtttaat tgatttattt attttaattg attgattata aattacataa
     3541 ctaaaaaatt aattctgaac agaattcatg cacgaactat atcctcttaa atttgagcca
     3601 attctgaaag aaaaagtatg gggaggcaac tcgctggtac ttgaatatgg aaagaaagca
     3661 gacccatcga agcttattgg agaaagctgg gagatttcag cagtagccga tcatctttcg
     3721 gtagtatcca atggcttcct tgccggaaac aacattgaag aaataataga agtctacatg
     3781 agcgatatca ccggtgaagc tgtttacgaa aagtttggga ttgagtttcc tttgctgatt
     3841 aaattcattg aagcttcttc cgacctgtcc gttcaggttc atcccgacga caggattgca
     3901 aaagacaagc acaatgcata tggaaaaact gagatgtggt atattcttaa agcccttccg
     3961 ggatcaaaaa tatacacagg tttcaaggaa ccccttaccg aagagaaact cagagaagcc
     4021 atttacgctg gcaaactgcc tgaacttttg aatatagaat atccacagcc aggagatgtc
     4081 ttttttacac cagcagggag gatacatgca attggaggtg gcataacact tgtagaaatc
     4141 cagcagactt ctgatatcac atacagaatt ttcgactgga acaggactgg tcaggatggc
     4201 aaaccaaggg aactccatgt agaatatgca attgatgcca tagattacaa cgccaccggc
     4261 tccgcactta taagaaaaga accggtgcca gataaaacaa taaaccttgt tacatgcgat
     4321 tattttgtaa caaacattat tcatctcaca ggggtattac gaaaagacta taatcttatt
     4381 gattcatttg taatttacat ttgcacagaa ggaaaatttt ccgtacattt tggttcaggt
     4441 accgaaacgg cagtgaaagg agaaacgctg ctggttccgg cggctattac tgatgtaatt
     4501 cttgaagcac agccggaagc agtaatactt gaggttttta ttaaatccca aaaaatcaac
     4561 gaatcatgaa aaagatttct tttcttattc tggcttttct gagtgtggtt tattttaccg
     4621 gatgcctgaa aaaaagccag caaaaacagg cagagaaaga aacacaggaa gaggtctctg
     4681 ttcctgatac tggttataca ggtattaagc aatactacag cggtaatttt ctagcatatg
     4741 aggttgaatt caaaaacgga gtcaggcacg gactgatgaa gacttattat tatccatcag
     4801 gtaagctgcg tcagactttc tggtatcaga acggtatgag ggaagacaca gcagtatggt
     4861 actacgagga tggtagggtt ttccgtaaaa caccctataa aagagacacc atgcacggag
     4921 tccagatcca gtattacaaa tcgggaagaa taagggcaaa acttgaattt gaaaatggtc
     4981 tgagaaaacc ttaccttgag gagtttacaa gcgacggcaa aaagataacc gactatcccg
     5041 aagttgtgat aaagaccgaa gacaactatg gtaaaaatgg aacctataaa atattgttga
     5101 gtctttcaaa gaaaaatgta aaagtcaatt tctacagggg aggttatatt aacggccttt
     5161 tcgaccccgg caaactggta aagatcaatg attctgacta tactggtgta ctgacactga
     5221 aaaaaagcgg ggtacagggt gaaaattatg tggagattat tgcagaaata acaacacctc
     5281 ttggaaacaa actgctggtt aacaaaaaga ttgacttacc ttataacgac cttcgctgat
     5341 aaagagaagg tggtcaaata agaaaaatat tatccggtat ggtaattcgg actgtaaaat
     5401 ttttttctgc aagtagcagc ctttctaaat gtccgcctgc cagtaaacct gaatttgcat
     5461 tcataggacg ttccaatgtg ggcaaatcat cactaatcaa tatgctggca ggtaacagaa
     5521 aacttgcaaa aatatctcag acacccggta agacaaggac tataaatcat tttctggtga
     5581 acaacgagtg gtttcttgta gaccttcccg gttatggcta ttcaaagatg ccttaccatg
     5641 agagaaagaa atggctgcag gaaactggca attatatact gaagcgcgaa accctggtct
     5701 cactttttgt tcttattgat gtgaggatag aaccgcagga aacagatttg aggtttatgg
     5761 agatgctggg cgtcaacaaa gtaccttttg ccatggtttt tacaaaatcg gataagatct
     5821 cccgcgaacg ggtaaaaaga aatattgaac actacaacag ggtaatgtta gaaaagtggg
     5881 aagaattacc tcctgaattt gtctcctcag ctgtgactaa agccggaaaa gaagagatac
     5941 tgaactttat agaagaatct atcaacaatt taaaaaaaag taaatgaatt ctgccggatt
     6001 ttatactttt gtaacgtcag taatccctga accgaaatac taaaagatat gtttggcaaa
     6061 gcacccatga aaatttttgc ctgcagggca tcaagagcac ttgcagaaaa tattgcctct
     6121 gaacttggag ttgaattagg aaaaagttca gttacagttt ttagtgatgg tgaatttcag
     6181 ccatgctacg atgaatcggt aagagggtgt cttgtattca ttgtacagtc aaccaatccg
     6241 ccggcagaaa accttctcga actgctgctt atgattgatg ctgcaaagag agcatcagca
     6301 tacaaagtaa ttgcagtgat cccatattat ggttttgcaa ggcaggacag aaaagatcga
     6361 ccgagagttt caataggttc gaaactttct gctgacctcc ttagtgctgc aggtgtcgac
     6421 cgcataataa caatggatct gcatgccgat cagatacagg gtttctttaa tgttcctgtc
     6481 g
//
LOCUS       DAOSNB010000145         6450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12104_length_6450_cov_4.641439, whole genome shotgun sequence.
ACCESSION   DAOSNB010000145 DAOSNB010000000
VERSION     DAOSNB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12104_length_6450_cov_4.641439"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="sprA"
                     /locus_tag="PLG42_06840"
     CDS             complement(<1..1095)
                     /gene="sprA"
                     /locus_tag="PLG42_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU30961.1"
                     /translation="MHPGYHLLGYNDRNFFDRAAANGWLSTDTLLNTPATFNNGQTIS
                     ARALVEPFPGLRIDLTADRRFNEAVSAYYIADKNGNFPDSTRNRIVTGNFTISVISWG
                     TAFEKISKKNDYDSPTYEAFRKYTEIISARRGEERRNIDPGYNPDVDPVTGNPIEGPY
                     KNGYGKTSREVLIPAFLAAYTKTDPKKVTLETFPSALKMMPNWRINFDGLSRFSFIQS
                     VFRSVNLSHQYRSTFSISSFTTNLKYSPDDKGVSQVRDLQYNFIPEYEINVVTINEQF
                     SPLINIDMNWKNSLTTRLEWRKSRTIALNLTSNQIADARTNEIVFGAGYRFDNVQIII
                     NTGGRQRALKSDLNVRFDFSVRDNKTIARKI"
     gene            complement(1064..6436)
                     /gene="sprA"
                     /locus_tag="PLG42_06845"
     CDS             complement(1064..6436)
                     /gene="sprA"
                     /locus_tag="PLG42_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOU30962.1"
                     /translation="MQFGKLTVTTVMSQQKGESSVLEVKGGAQLSTFDIPADEYEANR
                     HFFLSQYFRDIFDDAMKNLPVISSGINIERIEVWITNKTSRFEEGSNRNIVAFMDLGE
                     NASHIYNKVPAFQAKPGAKPNPDNSVNNLYDELVNNYPDVRETDRITDAFSSLYPDFQ
                     IGRDFEKIENARKLSEREYTLNKQLGFISLNTALNTDEVLAVAFEYTMNGQVYRVGEF
                     STDGINAPKALIVKLLKGTTLSPRLPTWKLMMKNIYSIGAGRVERKNFELQVLYQDDK
                     TGNAINYIPEGNLSNKILLQVLRLDNLNSQLDREPDGVFDFIEDATIMSARGRIIFPV
                     NEPFGSYLRKQFNDPKIADKYVFQELYDSTQTIARQIAEKNKFRLVGQYSSASGSEIQ
                     LNATSIPQGAVKVTAGGVVLTENIDYTVDYNLGTVRIINQALIDSETPIQVSLESNQF
                     FGFQTKTLVGTHLDYRISENFNIGGTILHLNERPFTQKVNFGEEPISNTIWGMNASYR
                     SQSQLLTNLVDKLPFLNTKAPSAISFFGEFANLIPGHSRAISSAGNSYIDDFEASEIP
                     LDLKSFNAWSIASIPQGQDQLFPEARLNNNLASGFNRARLAWYVIDPLFLRNGSSTPS
                     HIKENPDEQSSHFVREVFETEIFPYKESPSGVPTTIGVLNVAFYPSERGPYNFDTYPS
                     AFSAGLNSDGSLRNPRSRWGGMMREILTSDFEAANIQYIKFWLMDPFVENTEHKGGDL
                     YINLGNISEDILKDSRKSFENGLPGSPEVKNVDTTAWGRVPTVQAVVHAFDNDPQSRQ
                     YQDVGLDGLKDNDEGTFYEDYIQKLQSILTQEAYEEFVKDPSGDDFHYFRGSDYDRLR
                     LGILDRYKRYNGMEGNSPTAEMSPENYPTSGSTLPDMEDINRDNTLSETESYYQYRVS
                     LRPEDMVVGKNFIVDEIEYTATFANGQKSKVKWYQFKIPITDYEKVIGAIRDFKSIRF
                     MRMFLRNFEEPIIMRFAKLDLVRAEWRKYNIAFKEGSERITIPEQEDGSFEISSVSIE
                     ENSGKLPVNYVLPPGFTRIIDPSNPQLRQLNEQSMVLRVHNLEDGDARAAFKNVNLDI
                     RQYRQLRMEVHAEALIGEPLMDNELTAFIRIGSDYKGNFYEYEIPLKLTPPGRYNNNN
                     DSHRAMVWPEENRFIIDLSIFQEAKQARNRAMSEPGSTLSVSDVFIYNQGNHRISVSG
                     NPNLSNVKVIMVGVRNPSKTRNPAMDDGRPKSGEVWVNELRLSDFVENSGWAANAHLQ
                     ARLADFGIVDMVGQTSTPGWGSIEKKVNERSKEQVVKYDVSSTLEMGKFFPEKANIRL
                     PVYLGYSEAVIKPQFNPLDPDVPLKSALVEARNKAEKDSIKSISEDYSRRKTLTISNA
                     GVYIRGKRPHMWDPANLSFNYTYNEVYRSNIKTEIDLEKNIRGGINYNFEGQPANIAP
                     FKNMRFLNFSALRLIKDFNFYVFPRSISFRTDLSRYYNEVITRNINNPFLKVTPSYRK
                     DFEWSRIYDIKYDITRQLKFDFTATSIARIDEPPGGVDKRRYQSEYENWKDSVLTNLK
                     NFGRTTTYSHFININYNLPVNKLPLLGWTNANLRYGADYTWLAGPLYPDTMNINLGNT
                     IKNHNELTFTASGAFTTLYNKLKFLRDIENNTRPDARLRMRPEFKTVTYTREKVSFRP
                     GVARSITHNLKTQDVSVKVYDSSGREIKGKMSIVNENRITFAADEEVNNARVVVEGQV
                     VKKRSPAIVAGEYFVRALLGLRNVSLTFTNSQGQILPGFMPETNFLGMSRYNNLLAPG
                     LPFIRL"
BASE COUNT         1686 a         1460 c         1231 g         2073 t
ORIGIN      
        1 aattttccgg gctatagtct tattgtctct taccgaaaaa tcaaatctaa cattaagatc
       61 acttttcagt gccctctgtc tgccacctgt attaattatt atctggacat tgtcaaatct
      121 ataccctgca ccaaatacaa tttcgtttgt tcgtgcatcg gcaatctgat tgcttgtcag
      181 attcaacgcg attgtcctgg actttctcca ttccagcctt gttgtaaggc tgtttttcca
      241 gttcatgtca atatttatca gagggctgaa ttgttcattt atggttacta catttatttc
      301 atactcaggg ataaaattgt actgaaggtc tcttacctgg ctaacgcctt tatcatcagg
      361 tgaatattta agattagtag tgaatgaact tatcgaaaaa gtacttctat actggtgcga
      421 caggttaaca gatctgaaga ctgactgtat gaaactgaat cgagacaagc cgtcaaaatt
      481 gattctccag ttaggcatca ttttcaatgc ggatggaaaa gtttcgagag taaccttctt
      541 cgggtctgtc ttcgtgtatg ctgcaaggaa tgccggaatt agaacttccc gggaggtttt
      601 gccgtatcca tttttataag gaccttcgat aggatttccc gttacagggt caacatccgg
      661 gttataacct gggtcaatat ttcttctttc ttcacccctc ctggccgaaa ttatttctgt
      721 atattttctg aatgcttcat atgtgggtga atcataatcg ttcttcttgg atattttctc
      781 gaatgctgtc ccccacgata taactgagat agtgaagtta cccgttacaa ttctgtttct
      841 tgtactgtcg gggaagttgc cgtttttgtc agcgatataa tatgccgaaa cggcctcatt
      901 aaaccttctg tcggcggtga ggtcgattct gagacccggg aaaggttcaa caagtgctct
      961 tgctgaaata gtctgcccgt tattaaatgt agccggtgta ttcaggagtg tatcggttga
     1021 aagccatccg ttggcagcag ctctgtcaaa aaagttcctg tcgttatagc ctaataaatg
     1081 gtaacccggg tgcaagtaag ttattgtacc tgctcattcc gagaaaatta gtttcgggca
     1141 tgaatccggg cagtatctgt ccctgactgt ttgtaaaggt aagagacacg tttctgaggc
     1201 caagcaaagc acgtacaaag tattctccag caactattgc cggactgcgt ttctttacaa
     1261 cctggccttc aacaaccacg cgtgcattgt taacctcctc atcggccgca aaggttattc
     1321 tgttctcatt tactattgac atcttgccct taatctccct gccagagcta tcgtaaactt
     1381 ttaccgagac atcctgtgtt ttaaggttat gggtaattga acgggctact cccggtctga
     1441 aactcacttt ttcccttgta taagtgacgg ttttaaattc cggcctcatt ctcaggcgtg
     1501 cgtcaggcct tgtattgttt tctatatccc gcaggaactt caatttatta taaagtgttg
     1561 taaatgctcc gctggcagta aaggttagct cattatgatt cttaattgta ttcccgaggt
     1621 taatattcat tgtatcgggg tataacggac ctgccagcca tgtataatcg gcaccgtaac
     1681 gaaggttggc atttgtccac cccagcagtg gaagcttgtt tacaggaaga ttgtaattta
     1741 tattgataaa atgactgtat gtggttgtac ggccaaaatt tttaagattt gtaagcactg
     1801 aatccttcca gttttcatat tccgactgat accttctttt atcaactcca cccggtggct
     1861 catcaatacg tgcaatactg gttgccgtaa aatcaaactt caactgcctg gtaatatcat
     1921 atttaatatc ataaatcctg ctccattcaa aatctttacg gtaagaagga gttaccttca
     1981 ggaacggatt atttatattc cttgttatta cctcattgta atatctgctc aaatctgttc
     2041 tgaatgaaat actacgggga aatacataga agttaaaatc ctttattagt ctgagggcag
     2101 aaaaattcag aaaacgcatg tttttaaatg gtgctatatt ggcaggctgg ccttcgaaat
     2161 tataatttat tccacccctg atattctttt ccaggtcaat ctcagtcttt atattgcttc
     2221 tgtaaacttc gttatatgtg taattgaagc tcaggttggc cggatcccac atatgtggcc
     2281 ttttacccct gatatataca cctgcattgc tgattgtcag agttttccgt ctcgaataat
     2341 cttctgatat tgatttaatt gaatcctttt cagctttgtt tcttgcttcc accagagcgc
     2401 tcttaagagg cacatcaggg tcgagaggat tgaactgtgg ttttattaca gcttcggaat
     2461 accccaggta gacaggcagc cttatattgg ctttttcggg gaagaatttt cccatctcaa
     2521 gagtggatga tacatcatat tttacaacct gctccttgct tctttcattc acttttttct
     2581 caatacttcc ccatccgggg gtgcttgtct ggccaaccat atcaactatt ccaaagtcgg
     2641 caagtcttgc ctgaaggtgt gcatttgcag cccaaccgct attctccaca aaatcactca
     2701 atcttaattc attaacccat acctcacccg actttggccg gccatcatcc atggcaggat
     2761 ttcttgtctt tgaaggattt ctgacaccta ccataatcac tttgacattg ctcagattcg
     2821 gatttccaga tactgaaatt ctgtgatttc cttgattata tataaaaacg tctgaaacac
     2881 tcagggtaga acccggttcc gacatcgccc tgtttcttgc ctgcttggct tcctgaaaga
     2941 tagaaagatc aataataaat ctgttctctt caggccatac catggcacga tgactatcgt
     3001 tattgttatt ataacgcccg ggaggagtaa gcttcaaagg tatctcatat tcataaaaat
     3061 tgcctttgta gtccgaacct attcttatga acgctgttag ctcattatcc atcaatggct
     3121 cccctataag tgcttcagca tgaacctcca ttctaagctg cctgtactga cgtatatcaa
     3181 gattcacgtt cttgaatgcc gcccttgcat caccatcttc cagattatgc accctcaata
     3241 ccatagattg ctcattaagt tgccgcagct ggggatttga ggggtctatt attcttgtaa
     3301 accccggagg taaaacataa tttaccggaa gcttgccgct gttttcctca atactgactg
     3361 aacttatttc aaaagatcca tcttcctgct caggtattgt tattctttca cttccttcct
     3421 taaaagcaat attgtatttc ctccattctg cccttacaag atcgagcttt gcaaacctca
     3481 ttattattgg ctcttcaaag ttgcggagga acattcgcat aaatcttatc gacttaaaat
     3541 cccttatggc tccaatcact ttttcataat cagttatggg aattttaaac tggtaccatt
     3601 tcacttttga tttttgtcca ttggcaaacg ttgctgtata ctctatctca tcaacgatga
     3661 aatttttccc gacaaccata tcctctggcc tcaggcttac cctgtactgg taatagcttt
     3721 cggtctcgct cagtgtatta tccctgttta tgtcttccat atcaggaagt gtggagccac
     3781 ttgtcggata attttcaggc gacatctcag ctgtaggcga gttgccttcc attccattgt
     3841 accgtttgta gcggtctaga attcccagcc tcagcctgtc ataatccgaa cctctgaaat
     3901 aatgaaaatc atcacccgaa ggatctttaa caaactcttc atatgcttcc tgagtgagaa
     3961 tcgactgaag tttctgaata taatcttcat aaaatgttcc ctcatcattg tctttcagtc
     4021 cgtcgagacc gacatcctga tactggcgtg attgcggatc attgtcaaaa gcatgaacca
     4081 ctgcctgcac tgtaggtacg cggccccatg cagtagtatc aacatttttt acttccggag
     4141 aaccgggaag gccgttttca aaactcttcc ttgaatcttt aagaatatct tcagaaatat
     4201 tgccgaggtt aatgtacaga tccccaccct tgtgctcagt attctccaca aaaggatcca
     4261 tcagccagaa ctttatgtac tgaatattgg cggcttcgaa atcgcttgta agtatctccc
     4321 tcatcattcc accccatctc gaacgcgggt tcctgagcga accatcgcta ttgagccctg
     4381 cagaaaaagc cgaaggatag gtgtcaaaat tataaggccc tctttcagaa ggataaaagg
     4441 ccacattaag cactcctata gttgtcggca cgccactcgg actctcctta tatgggaata
     4501 tctccgtttc aaaaacttct cttacaaaat gactcgactg ttcatcggga ttttccttaa
     4561 tatgcgaggg agtggaagag ccgttgcgaa gaaaaagggg atcaatcaca taccatgcaa
     4621 gacgggccct gttaaatccg ctcgccagat tattattgag cctcgcctca gggaatagct
     4681 gatcctgacc ctgtggtatg ctcgcaattg accatgcatt gaacgatttc agatcaagag
     4741 gtatttcact tgcctcaaaa tcatcaatat aagaattacc agcacttgaa atggcccttg
     4801 aatggcccgg aataagattt gcaaattctc caaaaaatga aattgccgac ggggccttcg
     4861 tgttaagaaa cggcagttta tcaaccaggt tggtaagtaa ttgcgactgc gacctgtatg
     4921 aggcattcat accccagatt gtgtttgaaa ttggctcttc tccgaaattt actttctggg
     4981 taaagggcct ttcgttaaga tgcaatatcg tacctccaat attgaaattc tctgatatcc
     5041 tgtaatccag atgtgttcca acaagtgtct ttgtctgaaa accgaaaaac tggttacttt
     5101 caagggatac ctgaatgggt gtttcagaat cgatgagggc ctgatttatt attctcaccg
     5161 ttccgagatt gtagtcaacc gtataatcaa tgttttcagt aagcacaaca cctcctgcag
     5221 tcactttaac agcaccctgc gggatacttg tggcattcag ctgtatttct gaaccggagg
     5281 cagaagaata ttgacctacg agcctgaatt tgttcttttc agctatctgc cttgctatag
     5341 tttgagttga gtcgtaaagt tcctgaaaaa catatttatc tgcaattttc ggatcattaa
     5401 actgcttccg gagataactc ccgaaaggtt cattaaccgg gaaaattatt ctaccccttg
     5461 ccgacattat tgtagcatcc tctataaaat caaaaactcc atcgggctcc ctgtcgagct
     5521 gggaattaag gttatcaagc cgaaggacct gcagcagaat cttatttgag agatttcctt
     5581 cggggatata atttatagca ttaccggttt tatcgtcctg ataaagaacc tgaagctcaa
     5641 aattctttct ttcaaccctg ccggctccta ttgaatagat gtttttcatc atcagcttcc
     5701 aggtaggtag tcgcgggctt agtgtggttc cttttaataa tttaactata agtgctttag
     5761 gtgcatttat tccatcggtt gaaaattcgc ccaccctata aacctgtcca ttcattgtat
     5821 actcgaaagc cacggccagt acttcgtcag tattaagagc tgtattaagg gatataaatc
     5881 caagttgttt gttcagagta tactcccttt cgctcagctt tctcgcattt tctattttct
     5941 caaaatctct tcctatctga aaatcagggt acaggctgct gaaagcatct gtaatcctgt
     6001 ctgtttctct tacatcagga taattgttta ccaattcatc atacaggttg tttaccgaat
     6061 tatcgggatt gggtttggca ccaggctttg cctgaaaagc aggaacctta ttgtatatat
     6121 gggaagcatt ctcaccaagg tccataaagg ctactatgtt tctgttgctt ccttcctcaa
     6181 acctgctggt cttatttgtg atccacactt ctatcctttc gatgtttatg cctgaactta
     6241 tgaccggcaa atttttcatg gcatcatcga aaatgtccct gaaatattgg gagaggaaaa
     6301 aatgcctgtt agcctcgtat tcatcagcag gtatgtcgaa tgtgctaagt tgtgcgcctc
     6361 cttttacctc taaaaccgac gattcacctt tctgctgcga cattactgta gttacagtaa
     6421 gcttaccgaa ctgcatctcc gttttcagtc
//
LOCUS       DAOSNB010000146         6445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12117_length_6445_cov_3.837872, whole genome shotgun sequence.
ACCESSION   DAOSNB010000146 DAOSNB010000000
VERSION     DAOSNB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12117_length_6445_cov_3.837872"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PLG42_06850"
     CDS             complement(<1..1288)
                     /locus_tag="PLG42_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30963.1"
                     /translation="MMYRIFYIAIFIICAVFNEVFGQADNDPPDSPLLNLVTVQPESG
                     FTELYWKKSIAPDVAGYVIYYFKNNEGFAIDTIFNPEANSYLNVGSGSKFRSEAYVIA
                     ALDTAGNVSPLSNSLSTIFVEVAPDTCRRMLKIKWNFYEPIPYKVNEYRVIVTENGGN
                     SYEAGKVNSGENHFDLNNIHNGVNYCVVVEALLENGQKSSSNKQCLQVKMQKDPEWIN
                     ADYATVGDNGEILLSFSVDPESEIRSYRLERKREGESEFSFVKDFTINVSNNIRYTDN
                     TADISKKHYYRMAAINNCGIPSVYSNIAGNMVAEIKYSGDIMTLSWNLYKGWRGGILN
                     QEVAADFGEGPGEFAIILTQDSSMSIRYADLMYMISGPEICFYIIANELPNELGIAGT
                     SRSNKVCLPVEEKVFVPNTFTPDNDNINDLFKPVLSF"
     gene            complement(1285..2568)
                     /locus_tag="PLG42_06855"
     CDS             complement(1285..2568)
                     /locus_tag="PLG42_06855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU30964.1"
                     /translation="MKTQKSDRPPVNPINIEFISQPEKIILSNKVPVYFIKGGNEEIT
                     KIDFVFRAGFTAEEIPLQSSTTARMLTEGSEHMTASEINRELDYYGITFNAASDRDST
                     IISAIFLNRHFTKALELLKEIIFSPRFPEDEFKTLMNRRLQWFLINREKVSTIATEYF
                     FESVFGKSHPYGLRLNAEDFSRIKPAHLRNFHSRYYIPQEMTLFVSGKTPTGVIEHLE
                     NYVGSIVHYPADRKKSPPLPYGEKKRNLFIPKEGAVQSAIRIGSQTIKRSNPDFSSLL
                     IVNTLLGGYFGSRLMKNLREEKGYTYGISSYLVSLDLAGYLLIATEVGKKYTQKAVKE
                     IFKEIKILQKEPVKDKELEMVKNYMLSDLVRMFDGPFATAESFRLVWESGMDMSYFQT
                     LAQKIKSITPDEIIHIARSYYDTGELYTVIAGPEK"
     gene            complement(2565..3812)
                     /locus_tag="PLG42_06860"
     CDS             complement(2565..3812)
                     /locus_tag="PLG42_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOU30965.1"
                     /translation="MIEIKRCCLNNGLRVIVHEDSSTPLAAVSLVYMVGSRNEDPELT
                     GLAHLFEHLMFRGSESVPDFDDQLQLAGGENNAFTNSDITNYYLTIPAVNIETAFWLE
                     SDRMKGLNITEESLSNQKMVVLEEYRQAYLNQPYGDATLLIRPLAYKIHPYRWPTIGK
                     EEHIKKVTLGDIKEFFHSYYAPNNCIMVVAGNVSADDVFRLAEKWFGKIKMRKVHTET
                     LPEEPPQKSARKLTVKRNVPANALYKAWHVCCRRDPSFITLDLLTDILAAGESGRLQR
                     ILVREKKLFSSINAYLTGDLDPGLLIIQGRLNNGVSYEKAENELNKIIEEIKSTPVPD
                     EEMEKVKNRFEAEHIMDRVNILGKVLSLSQYEVLGDASLINSEIELYRNVTAGMVSEA
                     AGNYLKKTSCNTLYYISTAAKQL"
     gene            3918..5690
                     /locus_tag="PLG42_06865"
     CDS             3918..5690
                     /locus_tag="PLG42_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOU30966.1"
                     /translation="MKFEEQIKKPLNKIVSRLSQTQLIYLLSFIVGLLGALIASVMKN
                     TIHFTAELVNSSFSTGKGLLFYFIIPFTGVVLTYFFVKTFVKDNISHGISRVLHAISK
                     KKSYLKAKNIWASVIAGTITIGFGGSVGAEAPIVLAGSSIGSSIGRFFNLNYRQITLL
                     LGCGAAGAVSGIFKAPIAGIVFTLEILMLDLTMSSIVPLLISSVTAATVAWISMGDQV
                     LLSFSVKEAFLINNVPFYLILGIFTGLVSVWFSKMTMITEKLYNKINNQGLKLLAGGT
                     ALSVLVLLFPSFFGEGYNSVKSLLDGNESVLNSVILGFLPDKWWAILIISGLIVLLKV
                     FATSSTNAAGGIGGVFAPVLFTGAVAGFFSSKIIMHYSTAEIPDNRFVLAGMAGMMAG
                     VMHAPLTAIFLIAEITGGYDLLIPLIITSTISYITARAFTRYSIYHVQLATQGELITH
                     HKDKAVLVITDWRKDIETDLICVKPDDTLRELVKAIKRSKRNIFPVVDEYGVLEGVVL
                     LDNVRELMFNPDLYDEVLVRDLMTIPTTYIDVSESMASVMEAFNRTGEWNLPVLDKGI
                     YVGFISKARIFATYRDMLLQMSED"
     gene            5921..>6445
                     /gene="glyA"
                     /locus_tag="PLG42_06870"
     CDS             5921..>6445
                     /gene="glyA"
                     /locus_tag="PLG42_06870"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOU30967.1"
                     /translation="MKRDEVVFEIIRKEYERQAEGIELIASENFVSQQVLEAMGSVLT
                     NKYAEGYPGNRYYGGCEFIDMAEQVAIDRLKMLFGAEYANVQPHSGAQANMAVFMAYL
                     KPGDTFMGLNLSHGGHLSHGSPVNSSGILYRAVSYGVNEETGLVDYDMMEEIAHRERP
                     KLIIGGASAYSREWD"
BASE COUNT         1814 a         1229 c         1273 g         2129 t
ORIGIN      
        1 taaatgataa tacaggcttg aaaaggtcat ttatgttatc attgtcaggc gtaaaagtat
       61 tgggaacaaa aactttttct tctacaggca aacagacttt gttggatctg ctggtgccag
      121 caatacccag ttcattaggc aattcattgg caattatata gaagcatatt tccggccctg
      181 atatcatgta cattaaatca gcgtatctga tgctcatgga agaatcttga gtcaggatga
      241 tggcaaactc acctggtcct tcaccaaagt ctgccgccac ttcctgattc aatatccctc
      301 cacgccatcc tttgtagagg ttccacgaca acgtcataat atccccggaa tattttattt
      361 ctgcaaccat attaccggca atatttgaat ataccgatgg aatgccgcaa ttatttatgg
      421 cagccatcct gtaataatgc tttttggaaa tatcagccgt attatctgta tatcttatat
      481 tgttgctcac attaattgtg aaatctttca caaaagagaa ttcagattca ccttcccttt
      541 ttctttcgag tctgtaactt cttatctctg attcaggatc aacagaaaat gatagaagta
      601 tttctccgtt atcgccaaca gttgcataat ctgcatttat ccattcagga tctttctgca
      661 ttttaacctg caagcactgt ttgtttgatg atgatttctg cccattttca agaagagctt
      721 ccacaacaac acaataattg actccattat gtatgttatt caaatcgaaa tggttttcgc
      781 cggaattgac ttttccggct tcataactat ttcctccatt ttcagtgact attaccctgt
      841 attcattaac tttataaggg attggctcat agaaattcca tttaattttc agcattcttc
      901 tgcaagtatc tggagccact tcaacaaata ttgttgaaag cgaatttgaa agcgggctca
      961 catttccggc tgtgtcaagt gctgctatca cataggcttc ggatctgaac ttcgagcctg
     1021 aaccgacatt taagtaacta tttgcctccg gattaaagat agtatcaata gcaaaccctt
     1081 cattgttttt aaagtaatat attacatatc ctgccacatc gggtgcaatg ctttttttcc
     1141 agtaaagctc tgtaaagcct gattctggct gcactgtaac cagattgagc aaaggagaat
     1201 cgggtgggtc gttatctgcc tgcccgaaca cttcattgaa aacagcacaa ataataaaaa
     1261 ttgcaatata aaatattctg tacatcattt ttccggtccg gcaattactg tatagagctc
     1321 acctgtatca taatacgacc gggcaatatg aattatttca tcaggagtga tagattttat
     1381 cttttgagcc aatgtctgaa aatatgacat atccataccc gattcccata ccagcctgaa
     1441 actctctgcc gtggcaaaag ggccgtcaaa cattctcaca aggtcgctca acatgtagtt
     1501 tttgaccatt tcgagttctt tatcttttac aggttctttc tgcaatattt ttatttcttt
     1561 aaagatctct tttacagcct tttgggtgta ttttttacct acttctgttg ctataagcag
     1621 ataacctgca agatctaatg agaccagata tgaacttatt ccgtaagtat aacctttttc
     1681 ctcacgcaga tttttcataa gtcgggatcc gaaatatcca cccagtaaag tattaacaat
     1741 cagaagggaa gagaagtcag gattggacct tttaattgtt tgagagccta tacgtatggc
     1801 agattggacg gctccttcct tcgggataaa caggtttctc tttttctctc catagggaag
     1861 aggaggagat tttttgcgat cagccggata atgcacaata cttcctacat aattctccag
     1921 atgttctatt accccggtgg gtgtttttcc tgatacgaac aaagtcatct cctgagggat
     1981 gtaatatctc gaatgaaagt tacggagatg cgctggttta attctggaga agtcttctgc
     2041 atttaacctc agcccataag gatgactttt cccaaataca gattcaaaaa aatattcagt
     2101 agcaatggta ctcactttct ccctgttaat taaaaaccat tgtaaccttc tgttcatcaa
     2161 tgttttaaac tcgtcttccg gaaaacgtgg gctaaaaatt atttccttta acaattccag
     2221 tgcttttgta aaatgtctgt tgagaaatat tgctgatatt atagtcgagt ccctgtcgga
     2281 tgcagcgttg aacgtaatgc catagtagtc aagttcgcgg tttatttcgc tggctgtcat
     2341 gtgctcagag ccttcagtga gcattctggc agtggtggag gattgcagcg gaatttcttc
     2401 agccgtgaat cctgccctga agacaaaatc aattttggta atttcctcat ttccaccttt
     2461 aatgaagtaa acaggaactt tgtttgataa aattattttt tccggctggc tgataaactc
     2521 aatgtttata ggatttaccg gaggtcggtc agatttttgg gttttcataa ctgttttgct
     2581 gcggtactta tataatataa tgtgttacaa cttgtttttt taaggtaatt tccggctgcc
     2641 tcacttacca tacctgcagt gacgtttcta taaagttcaa tttcgctgtt gatgagtgaa
     2701 gcatctccaa gcacttcata ctgagatagg gaaaggactt tgccgagaat atttacacgg
     2761 tccattatgt gctcagcttc aaatctgttt tttacttttt ccatttcctc atcaggtaca
     2821 ggtgtgcttt ttatttcttc aatgatctta ttgagttcat tttcagcttt ttcataacta
     2881 actccattat tcagcctgcc ctgaattata agcaaacctg gatccaggtc tccggttaag
     2941 tatgcattaa tactgctgaa aagttttttc tctctgacta aaattctttg caatctgccg
     3001 gattctcctg ctgccagaat atctgtaagc aggtcaagtg ttatgaatga aggatctctt
     3061 ctgcagcaga cgtgccaggc tttgtaaagt gcatttgccg ggacatttct ttttactgta
     3121 agttttcggg ctgatttctg tggaggctct tcaggaaggg tctctgtatg aaccttgcgc
     3181 atcttgattt tcccgaacca cttttctgca agccggaaaa catcatcagc actgacattt
     3241 cctgctacaa ccattatgca attgttgggc gcataatacg agtggaagaa ttcttttata
     3301 tcacccagtg ttaccttttt aatatgttct tctttaccga tagttggcca gcggtatgga
     3361 tgaattttat atgcaagcgg gcggatcaga agagttgcat caccataggg ctgatttaaa
     3421 taggcctgcc tgtattcttc aagaaccacc atcttctggt tgctgaggct ctcttctgtg
     3481 atgttgagtc ctttcatcct gtccgattca agccagaatg cagtctcaat atttacagca
     3541 ggtatggtga ggtagtaatt tgttatatca ctgtttgtaa aagcattgtt ttctcctcct
     3601 gcaagctgaa gctggtcgtc aaaatcgggc acactttccg aacctctgaa catcaggtgt
     3661 tcaaaaagat gagccagacc tgtaagttcc ggatcttcat ttcgcgatcc caccatataa
     3721 actagactta ctgctgcaag aggagttgag ctgtcttcgt gaactattac ccttaatcca
     3781 ttattcaggc agcatctttt tatttcaatc atcaggcaat attaatatga gtgaatgtcg
     3841 tatgagtgtt tatgttaata tgcaaacata ctaaaaatat gattttactt tgcttatgtt
     3901 tttatctttg catcgtcatg aaatttgaag agcaaattaa gaaacctctg aataaaattg
     3961 tctcccgact gagtcagaca cagcttattt atctgctgag ctttattgtg ggattgcttg
     4021 gtgcattgat tgcatcagta atgaaaaata caattcattt tactgccgag ctggtcaact
     4081 catcttttag caccgggaag ggtttattat tttactttat tattccgttt acgggtgtgg
     4141 ttcttactta tttttttgta aagacatttg ttaaggataa tatcagtcat ggcataagcc
     4201 gggttctaca tgccatatct aagaagaaga gctatcttaa agcaaaaaat atatgggcat
     4261 cggttattgc cggaacgata acaattggtt ttggcggctc ggtgggtgct gaggctccaa
     4321 ttgtgctggc aggctcatca atagggtcct ctataggacg tttcttcaat ctgaattacc
     4381 ggcagattac actcttactt ggttgcggtg cagcaggagc tgtgtcggga atattcaaag
     4441 ctcctattgc aggaatagtt tttacccttg aaatattgat gctcgacctg accatgtcat
     4501 caatagtacc gttgcttatt tcatcggtta ccgctgcaac agtagcctgg atatcaatgg
     4561 gagatcaagt gctcctctct ttttcggtga aagaggcctt tcttataaat aatgttccct
     4621 tctatttgat tctcggaatc ttcacaggtc ttgtttctgt ttggttctca aaaatgacta
     4681 tgataaccga gaagctgtat aataaaataa acaatcaggg attaaagctg cttgccggag
     4741 gaacagccct cagtgtactt gtgttgcttt ttccttcatt ttttggagaa ggatacaatt
     4801 ctgtgaaaag tttactcgac ggaaatgaga gcgtgttaaa ttctgtgata ttaggctttc
     4861 ttcctgataa atggtgggca atactaatta tatcaggttt gattgttcta ttaaaagtgt
     4921 ttgcaacaag ctctacaaac gcagcaggag gaattggagg agtttttgca cctgtattgt
     4981 ttacaggagc agtagcaggc tttttctctt ctaaaataat aatgcactat tcaacagctg
     5041 agatacccga taatagattt gttctggcag gaatggctgg tatgatggca ggagttatgc
     5101 atgcccctct aaccgcaatc tttcttattg ccgaaattac aggaggatac gacctgctga
     5161 tacccctgat tattacttcc acaatatcat acataacagc aagggcattt acccggtatt
     5221 caatatatca tgtacagctt gctacacagg gtgaacttat tacacaccat aaggataaag
     5281 cagtgctagt tataacagac tggagaaaag atattgaaac cgaccttatc tgtgttaaac
     5341 ctgatgatac actgcgtgaa ctggttaaag caattaaaag gtcgaaaaga aatatatttc
     5401 ctgttgtgga tgaatatgga gtgcttgaag gcgtggtcct tctcgataat gtcagagaac
     5461 ttatgtttaa tcccgattta tatgatgaag ttcttgttag agatttaatg actatcccta
     5521 caacatatat tgatgtatcg gaaagtatgg cctcagttat ggaagcattc aacaggactg
     5581 gagagtggaa cctgcctgtt cttgataaag gaatttatgt gggctttatt tcaaaagcaa
     5641 gaattttcgc aacctacagg gatatgctgc ttcaaatgtc agaagattga tttagatgaa
     5701 atttatgttt ttaaaacttt tacctaaaat tgcacaagta ttaaatagtt ttgatttaat
     5761 aattgaacat tgaattaatt ttatcatttc attcataact tgaatatgtt tgaaatttca
     5821 cataaaattt tgtcaaactt aaattatcac atactttaac cctgatagtg atttggaaaa
     5881 tcatgataat atattgaata ttaacataat acattgaata atgaaaagag acgaagtcgt
     5941 ttttgaaatt atcagaaaag aatatgaacg ccaggcagag ggtatagagc ttattgcatc
     6001 ggagaatttt gtgagtcagc aggtacttga agcgatgggt tctgttttaa caaacaaata
     6061 tgctgaaggc tatcctggta acagatatta tggaggatgc gaatttattg atatggctga
     6121 gcaggtagct attgacagac tgaaaatgct ttttggggca gaatacgcca atgtacaacc
     6181 tcattcaggt gcacaggcaa atatggctgt ttttatggct tatctgaaac ccggtgatac
     6241 atttatggga ctgaatcttt cacatggcgg gcatctttcg catggttctc cggtcaattc
     6301 ctccgggatc ctttacaggg ctgtatcgta cggggttaat gaggagacag gtctggtgga
     6361 ttatgatatg atggaagaaa ttgctcatcg agagaggcct aaacttatta ttggaggagc
     6421 ttcggcttat tcgagggagt gggat
//
LOCUS       DAOSNB010000147         6388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12262_length_6388_cov_4.846992, whole genome shotgun sequence.
ACCESSION   DAOSNB010000147 DAOSNB010000000
VERSION     DAOSNB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12262_length_6388_cov_4.846992"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2740)
                     /gene="nifJ"
                     /locus_tag="PLG42_06875"
     CDS             complement(<1..2740)
                     /gene="nifJ"
                     /locus_tag="PLG42_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOU30968.1"
                     /translation="MKTKKFIVCDGNQAASHIAYMFSEVACIYPITPSSTMAEYVDEW
                     AASGLKNMFGETVKVVEMQSEAGAAGAVHGTLQSGALCTTFTASQGLLLMIPNMYKIA
                     AELLPGVFHVSARTVAAHALSIFGDHSDIYATRQTGFAMLASGSVQEIMDLAGVAHLS
                     AIKSRIPFLHFFDGFRTSAEVQKIELLEMEDLKKLVDWDALAAFRNNALNPEKPVTRG
                     TAQNPDIFFQAKEAINRYYEPVADIVAGYMAEISKLTGRTYKPFDYYGHPEADRIIIA
                     MGSVTDTTKETIDYLTAKGEKVGLIAVHLYRPFSAKYFFDVLPKTVKVISVLDRTKEP
                     GALGDPLYLDVKSLFYDSELQPLILAGRYGLSSKDTTPAMILSVFENMKLKEPKNHFT
                     IGIIDDVTHTSLPMLPDIDVDYPGTYSAKFFGIGADGTVGANKNSIKIIGETTDKYCQ
                     AYFAYDSKKSGGLTVSHLRFGDKPIRSPYYVNNPDFVACHVPAYLNKYDMLKGLKKGG
                     TFLFNSIWDVEETKRRLPDHMKKYLAENKINFYMINATAIADELGLGTRTNTIMQSAF
                     FKVSNVIPYEKAVEEMKKAVKKTYGRKGEDVVKQNYDAIDRGGEVIKVDIPSEWADIK
                     IEQKPDTRDVPEFIKKIFDPINRMKGDEVPVSAFIGREDGTFPPGTTAYEKRGVAVHV
                     PEWQPENCIQCNQCSYVCPHAAIRPFLLTEEEAAKAPEGTKVLQAIPGSLKAYKFRIQ
                     VSVYDCVGCGNCADVCPAKTKALVMKPFETQLAEAERWTYMHEKVGYKDNVVDKFLNV
                     KNSQFAQPLFEFSGACAGCGETPYIKLITQLFGDRMTIANATGCSSIYGGSAPSTPYT
                     VNKNGHGPAWANSLFEDNAEYGFGMALGTNKLRERIARKMEEALSNGKVNSGTKAAF"
     gene            complement(2832..4634)
                     /gene="rho"
                     /locus_tag="PLG42_06880"
     CDS             complement(2832..4634)
                     /gene="rho"
                     /locus_tag="PLG42_06880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOU30969.1"
                     /translation="MYDILELSKKLVPELREIAKSLNIKRVESLKKQDLIYKILDQQA
                     LKSIEGKPLPKTEAAEEKDSGSKTEPSSRRGKRPRFIKSAGNSRESIITVTPDGLTRS
                     ETGTKDWHDNRNTEDKSDEAGRKKKQKYELFEAPEIIIPKSELHQKRREEEPYKKEEA
                     PVEKPKEKELLDDSLLSMPNGELVDEELKAEIEASEKELSAERALSEEIITAPEPKEK
                     EEKKERFYDFEGVIYTTGVLEIMPDGYGFLRSADYNYLSSPDDVYVSQSQIKLFGLKT
                     GDTVTGSIRPPREGEKYFPLIKIEEINGRSPDYIRDRVPFDYLTPLFPNEKFTLCTPG
                     EGSLSVRIIDMFCPIGKGQRGLIVAQPKTGKTILLQEIANAIAKYHPEVYLIILLVDE
                     RPEEVTDMARNVKAEVIASTFDEPAERHCRVANIVLEKAKRLVECGHDVVILLDSITR
                     LARAFNTIAPASGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIIATALIDTGSKM
                     DEVIFEEFKGTGNMELQLDRKLANRRIFPAIDIPASSTRREDLLLNKTILQKIWVLRN
                     YLSDMNSVEAMEFLRDRLAQTKSNEEFLFSMNGS"
     gene            4963..5826
                     /locus_tag="PLG42_06885"
     CDS             4963..5826
                     /locus_tag="PLG42_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOU30970.1"
                     /translation="MRQLKISKQVTNRDTPSLDKYLQEIGKVDLLSPEDEVNLARRIK
                     SGDNDALATLVKANLRFVVSVAKQYQNQGMTLPDLINEGNLGLMKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIIRLPVNKIGSLNRINRAFARLEQKYEREPSDQEIAD
                     MLEMAPDEVKDALKTNGRAISMDAPISPDEENTMYDIIQNNDSLSPDKNLINESLSFE
                     IERALSTLSPREAKVLKLYFGINMKHPYTLEEIGEVMKLTRERVRQIKEKAIKKIQYT
                     TRCKILKTYLG"
     gene            5875..>6388
                     /gene="rpe"
                     /locus_tag="PLG42_06890"
     CDS             5875..>6388
                     /gene="rpe"
                     /locus_tag="PLG42_06890"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOU30971.1"
                     /translation="MTHLVSPSILTADFGNLEKEIKMLNSSVADWIHLDIMDGVYVPN
                     ISFGFPVLESVKRIATKPLDVHLMIADAERYLHQFRKAGAEILTVHYEACTHLQRTVT
                     EIRALGMKAGVALNPHNPVFLLRNILPYIDMVLIMTVNPGYGGQTFIEESWNKITEMR
                     KMIDESGYNVM"
BASE COUNT         1757 a         1420 c         1306 g         1905 t
ORIGIN      
        1 cgaatgccgc ttttgttccg gaattaacct tgccattgct tagggcttct tccatcttcc
       61 ttgctatacg ctcccttagc ttatttgttc caagtgccat tccaaatccg tattcggcat
      121 tatcttcaaa gagtgaatta gcccatgccg gtccgtgacc gtttttgttt acagtataag
      181 gtgtggaagg agctgagcct ccgtagattg acgaacaacc tgttgcgttt gcaatggtca
      241 ttctgtcacc aaatagctgg gttataagtt ttatgtatgg agtttcacca cagcctgcgc
      301 atgctcccga aaattcaaag agtggctgtg caaactgact gttcttaacg ttgaggaact
      361 tatcaacaac attatccttg taccccactt tttcatgcat gtaagtccat cgctctgctt
      421 cggcaagctg tgtttcgaaa ggtttcatta caagagcctt ggttttggca ggacatacat
      481 cagcacagtt accgcatcca acgcaatcgt aaacactaac ctgtattctg aatttgtaag
      541 ctttcagtga tccggggata gcctgaagga ctttcgttcc ttccggagct tttgcagctt
      601 cttcttcagt aaggaggaaa ggcctgatag ctgcatgtgg acatacatac gaacactggt
      661 tgcattgtat gcagttctcc ggctgccact caggtacatg tactgccacg ccacgttttt
      721 cataagcagt ggtccccgga gggaaggtgc cgtcttcacg accaataaag gcacttaccg
      781 gaacctcatc tcccttcatg cggtttatgg ggtcaaatat cttcttgata aattcaggaa
      841 catccctggt atcaggtttc tgttcaatct taatgtcagc ccattctgaa ggaatatcaa
      901 cctttatcac ttctccaccc ctgtcaatag catcatagtt ctgttttaca acatcctctc
      961 cctttcggcc ataagttttt ttaacagcct ttttcatctc ttcaacggct ttctcataag
     1021 gtattacatt tgaaacctta aagaaagctg actgcattat agtgttggta cgggttccca
     1081 aaccaagttc atctgcaatg gcagttgcgt tgatcatata gaaatttatc ttgttttcag
     1141 caagatactt tttcatatga tcgggtaggc gacgctttgt ctcttcaaca tcccatatcg
     1201 agttaaacag gaaggtgccg cctttcttca gacctttgag catatcgtat ttgttaagat
     1261 atgcaggcac atggcaagca acaaaatcag gattgtttac ataataaggt gaacgaattg
     1321 gcttatcccc aaaacgtaga tgtgaaactg ttagtccacc tgattttttc gagtcgtaag
     1381 caaaatatgc ctggcaatat ttatcagtag tctcaccgat aatcttgata gagtttttat
     1441 tggcacctac tgtaccgtct gcaccgatac cgaagaattt tgcggaatag gttccgggat
     1501 aatcaacatc tatatcgggc aacattggga gtgatgtatg ggtaacatca tcaataatac
     1561 ctattgtaaa atgattcttc ggctctttca gtttcatgtt ttcaaatacc gacagaatca
     1621 tagcaggtgt tgtgtctttc gagctgagtc cgtaacggcc agcaaggatt aacggttgta
     1681 actcactgtc gtaaaagagt gattttacat caaggtataa aggatctccg agtgccccag
     1741 gttctttggt tcggtcgaga acagaaataa cttttacagt tttgggtagt acatcgaaga
     1801 aatattttgc cgagaagggc ctgtaaagat gaactgctat caaaccaact ttttcacctt
     1861 ttgcagtgag gtaatcgata gtttctttcg ttgtgtcggt tacagacccc attgcaataa
     1921 ttatcctgtc ggcttccgga tgtccataat agtcaaaagg tttatatgtt ctgcctgtaa
     1981 gcttcgatat ttcagccata tatccggcaa cgatgtcggc aacaggctca tagtatctgt
     2041 ttatagcttc ttttgcctgg aagaaaatat caggattctg tgcagtgcct cttgttacag
     2101 gcttctccgg attcagtgcg ttgtttctga atgctgcaag tgcatcccaa tcaacaagtt
     2161 ttttaagatc ttccatttcc agaagctcta tcttctgaac ttcagctgag gtacggaaac
     2221 cgtcgaagaa atggaggaaa ggaattctgg attttattgc tgagaggtgg gcaacaccgg
     2281 caaggtccat aatttcctga acacttccgc ttgcaagcat tgcaaagcct gtctggcgtg
     2341 ttgcatagat atcgctgtga tctccaaata ttgaaagtgc atgggcagcc acagtacggg
     2401 cactaacgtg aaaaacgccc ggcagaagct cagcagcaat cttgtacata ttgggtatca
     2461 tcagcaacaa accctgtgaa gcagtgaatg ttgtacagag ggcacccgac tgaagggtac
     2521 cgtgaacagc accggcagct cctgcttccg attgcatttc gactacttta actgtttcgc
     2581 cgaacatgtt cttcaacccc gaagctgccc actcgtcaac atattcagcc attgtcgacg
     2641 atggtgtaat gggataaata caggccactt cactgaacat atatgctatg tgcgacgcag
     2701 cctgattacc gtcacatact ataaattttt tcgttttcat tttttattta gttacctggt
     2761 tatttttaaa gatttagttt cagatgcaaa gttataaact gacagcgaga aaacaggaat
     2821 tttaatttta tttaactacc attcatcgag aaaagaaatt cctcgttcga ctttgtctga
     2881 gccagtctgt cgcgcagaaa ttccatagct tccaccgagt tcatgtcgct gagatagttg
     2941 cgaagaaccc atatcttctg cagtattgtt ttgttgagaa gcaaatcttc ccttctggta
     3001 ctggaagccg gaatatcgat tgccgggaat atccttctgt tggcaagctt gcggtcgagc
     3061 tgaagctcca tattaccggt gcccttgaat tcctcaaaga taacttcatc catctttgaa
     3121 cctgtgtcga tgagggctgt tgcgataatt gtaagcgagc ctccgttttc tatgttgcga
     3181 gcggcaccaa aaaacctctt tggtttatgc agtgcgttag aatctacacc acccgacagc
     3241 accttgcccg atgcaggggc aatggtgttg aatgcccttg ccagcctggt gatggaatca
     3301 aggagaatta ccacatcatg cccgcactcc acaagcctct ttgctttctc cagtacaata
     3361 tttgcaaccc tgcagtgcct ttctgccggc tcatcaaaag ttgatgcaat aacttctgcc
     3421 tttacatttc tagccatatc agtaacctcc tcaggccttt cgtcaacaag cagaattatc
     3481 agatacacct caggatggta tttggcaatg gcgtttgcaa tctcctgaag gagaatggtt
     3541 tttcctgttt ttggctgagc tactatcagg cccctctgtc ctttgcctat cggacagaac
     3601 atgtcaatta ttctcaccga aagagaacct tcaccgggtg tgcaaagggt gaatttttca
     3661 ttggggaaga gcggagtgag ataatcgaaa ggaactctgt ctcttatgta atccggactt
     3721 cggccattta tttcttcaat tttgattagc ggaaaatact tttcaccttc cctgggcggt
     3781 cttatggatc cggttacagt gtcgccggtt ttgaggccaa aaagttttat ctgcgactgt
     3841 gaaacatata catcgtcggg tgaactgagg taattgtaat cggccgacct cagaaaccca
     3901 taaccgtcag gcattatttc aagaaccccc gttgtataga taactccttc gaaatcatag
     3961 aacctttctt ttttctcttc tttctctttg ggttcaggag ctgtaatgat ttcttcactc
     4021 aaagctcttt cggctgaaag ttctttttct gaagcctcta tttcagcttt gagctcctca
     4081 tcaacaagct ctccattagg catcgaaaga agcgaatcat ccagaagttc cttctcctta
     4141 ggtttctcaa ccggagcttc ttctttctta taaggctctt cttctcttct cttctgatgc
     4201 aattctgatt taggaattat tatttccgga gcttcgaata actcatactt ctgcttcttc
     4261 ttccttccgg cttcgtcaga cttatcctca gtattgcggt tatcatgcca atctttggtg
     4321 cccgtttcag atctggtaag cccatcggga gtaacagtga tgattgattc ccgcgaattt
     4381 cctgcactct tgatgaaacg aggccttttt ccacgccttg acgaaggctc ggttttcgac
     4441 cctgaatcct tttcctcagc agcttcagtt tttggcaacg gcttgccctc gattgatttc
     4501 aatgcctgct ggtccagtat cttataaata agatcctgct tttttaatga ttcaactctt
     4561 ttaatgttta gagatttggc tatttccctt aattcgggaa ccagcttttt acttagttca
     4621 agaatgtcat acataatctg cttctgcgat gtgtaaaatt gattttttaa tttccatgcg
     4681 atgatccggg ttcagagaag accaataaac ccgaaagaat attatttaaa aacacgtgca
     4741 atattaaata aaattgctta taaatttcca aatttaaatc aaaaagtaaa tgcttgtctt
     4801 gtgaagttga tgttatcaac ggcaacgacg tgacaaaatt agtaaaattt ttttattgaa
     4861 aaaaatatta tcagaacttg tattgatgaa gaatattttt tttattaaaa ttgtaacctt
     4921 tggctggttt ttcatttttt tatttattcc tttgacctgt atatgcgtca gctcaaaata
     4981 tcaaaacagg tcacaaaccg tgacacacca tctcttgata aataccttca ggagatagga
     5041 aaggtcgatt tattgtcacc cgaggatgag gtaaatctgg caaggcgtat caaatcagga
     5101 gataatgatg cccttgcaac actggtgaag gcaaatctga ggtttgttgt ttcagttgcc
     5161 aaacagtacc agaaccaggg catgactttg cccgacctga taaacgaagg caacctcgga
     5221 ctgatgaaag cagcccagag gtttgatgag acaaggggct ttaagttcat ttcctatgca
     5281 gtatggtgga tacgccagtc tatactgcag gctcttgctg aacaggcacg tatcataagg
     5341 ctgcctgtga ataagatagg ttcgcttaac agaattaaca gggcattcgc caggctcgaa
     5401 cagaagtatg agagagaacc atccgaccag gagattgccg atatgctcga gatggctcca
     5461 gatgaggtta aagacgccct aaaaacaaac ggaagggcta taagcatgga tgcccctatc
     5521 agccccgacg aagagaacac aatgtacgac atcatacaaa ataacgactc tcttagcccc
     5581 gacaaaaacc ttatcaacga atccctgtcc ttcgaaatcg aaagagcact gagtaccctg
     5641 tcgccgcgcg aagcaaaagt ccttaaactt tacttcggaa taaatatgaa acatccttat
     5701 accctcgagg aaataggaga ggtgatgaaa cttacacgcg aaagagtgag gcagataaaa
     5761 gaaaaagcaa taaagaaaat ccagtataca acccgctgta agattctaaa aacatatctg
     5821 ggctgatgat ttttcaaaat tgtttgccaa atttactgcc ttaaacctat ctctatgaca
     5881 caccttgttt ccccttcaat attgacagcc gattttggca accttgaaaa ggaaataaag
     5941 atgctcaaca gcagcgttgc cgactggata cacctcgaca taatggatgg tgtctatgta
     6001 ccaaatattt cattcggctt tcccgtactg gaaagtgtaa aaagaatcgc aacaaaacct
     6061 ctcgatgtac acctgatgat agcagatgcc gaacgttacc tgcaccagtt cagaaaagca
     6121 ggagccgaaa tacttacagt ccattatgag gcatgtaccc acctccagcg aaccgtaact
     6181 gaaataagag cgctcggaat gaaagccggt gtggccctga accctcataa tcctgttttc
     6241 ctgctcagga atattctacc atatattgat atggtcctta ttatgactgt taaccctggt
     6301 tatggaggac agacatttat cgaagagagc tggaacaaaa tcacagaaat gagaaagatg
     6361 atagatgaat cgggatataa tgtaatga
//
LOCUS       DAOSNB010000148         6335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12394_length_6335_cov_4.677229, whole genome shotgun sequence.
ACCESSION   DAOSNB010000148 DAOSNB010000000
VERSION     DAOSNB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_6335_cov_4.677229"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="PLG42_06895"
     CDS             <1..1466
                     /locus_tag="PLG42_06895"
                     /inference="COORDINATES: protein motif:HMM:NF037199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HOU30972.1"
                     /translation="FNSVLVSVSWEIIEPSEGRFDFSSVDELLRIARENDLKVGFLWF
                     ASWKNGFSPYAPSWVLGDTRRFERVKDEKGRNTRTLSPLCNATRDADARAFAALMRHL
                     AQADSKENTVIVVQIENEVGVLRQTRDFSAAANKLIASQVPEELLRHLSKNRQTLEPE
                     LRTAWENNGAKTKGTWYEVFGAGDYTDMFFMAWQYARYINAVAEAGKREYNIPVYVNC
                     WMSGSRPNIGKPGNFPSGGPVLSVLDIWKAGAPAVDILAPDIYGADFKDQCRNFHRHD
                     NPLFVPETRAVEGAATWAFSTHDAICFSPFGIDTRPETMAAEYKMLSQLIPVITQYQG
                     TGLMSGFYRDGRDTTRNYEFKLNDDVKIIVTLQQAARRETSPTAPGTGNAQQRQQTPE
                     QGSYGLFIKTGESEFMVAGNNISVTAVSLNPKKEVWLKDAWEGTLVDGVWKPKVLLNG
                     DEAGFLRSDDPVYSVRAYKSTPPEPAIIKFRAVVYDR"
     gene            1564..2991
                     /locus_tag="PLG42_06900"
     CDS             1564..2991
                     /locus_tag="PLG42_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOU30973.1"
                     /translation="MITFISGILILLAGYFVYSRFIEKIAGADASRETPAYRMRDGVD
                     YIPMPWWRVFLIQFLNIAGLGPIFGAIAGAMWGPVAYLWIILGSVFAGAVHDYLSGML
                     SIYHNGLSITEIVGIYLGNGIKQFMRGFTVVLMVLVGAVFIVGPAGILGGMTQGTLSV
                     TFWVWVVFFYYIVATMLPIDKVIGRIYPFFGFALVFMAFGLITALFINGYKPPELTIS
                     TLRNFHVDSGKFPVFPMMFITIACGAISGFHSTQSPLMARCITNEKLGRRVFYGAMIS
                     EAIVAMIWATIGMSFWGGVKELNDIMTANKGNAAVLVNEVSNSLLGKFGGILALLGVV
                     AAPITSGDTAFRSARLIVADFLNFKQGPIKNRLIISIPLFAIGFLITKMDFSIIWRYF
                     AWCNQTLAMIVLWAETVYLVRAGKIYGITLVPALFMTAVSATYILIAPEGFRLAGNIA
                     YPAGAAATLAALTGFVAYLYRRRTK"
     gene            3109..3858
                     /locus_tag="PLG42_06905"
     CDS             3109..3858
                     /locus_tag="PLG42_06905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOU30974.1"
                     /translation="MKTRRKFIKETAAGAMAAMSIPKVLYEFPAGAGQKKIKLEKNNV
                     ILFQGDSITDAGRNKQNESFNNPSSLGSGYAFLATAALLESFPSLNLKVYNRGISGNK
                     VFQLAERWDKDCLDLKPDVLSILIGVNDIWHTLNGQYNGTVEKYRNDYIALLERTRKA
                     LPSVKLIICEPFAVPGVKAVDDRWYPEFYEYQKSAREIAERFGAIFVPFQKVFDEAQK
                     RAPGSYWTGDGVHPSLPGAQLMAKAWLKTVL"
     gene            3874..6081
                     /locus_tag="PLG42_06910"
     CDS             3874..6081
                     /locus_tag="PLG42_06910"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOU30975.1"
                     /translation="MLPKRYIISYRWQAFAFLTICLLYGNISFSQEEYNVLKESWLKY
                     TDAQNFLYHHLLSDAVFLLEKRKERTDSLATLFSWQSRQKEIREILQRITGPFPEKTP
                     LNARIVRTIEKPGFRIEHIIFESQPGFYVTSSLYIPAGIGKGQKLPAVIYCSGHSAEG
                     YRSAVYQHVIQNLVKKGFIVFAFDPVGQGERLEYPDPATGKSLVGGPTHEHSYPGSQT
                     LLTGTSQAAYMIWDGIRAVDYLLTRREVDPSRIGITGRSGGGTQSAYIAAMDDRIFAA
                     APENYLTNYTRLLESIGPQDAEQNIPKFIASGLDHADFLIVRAPKPAMMITTTRDMFS
                     IQGAVETEKEITRVYSAYNQPGNFSRVEDDTTHASTVKNRQALYTFFGKHLNNPCNNS
                     DEETIRLSPEELRVTSKGQVSFESGAETVHSLNLKRAIALEKRLDDSRNGEIKHFTDA
                     VDAAKKLSGFIEPESVGDPVFAGRFRKDGYCVEKYFIRGEGDYVIPFLLFVPDKQTSK
                     YLIYLHPEGKSAEAGAGKEIESIVLHGITVLAPDLPGTGETGPGSFRGDAYFAGVSHN
                     LLYAALLTGRSITGIYAGDIIRLAKFIEKRDEHADIKGFAKGYLSPALLHAAAFSHLL
                     KGIALVNPYISYFSLVKERMYNPLFMLTAVPGALKEYDLPDLAGALAPRKLLITGATD
                     GKGSSEDSEVINKGLSVIKAAYSVNNAGDKLRIYPITDKVHIINNLIDWLMKE"
BASE COUNT         1851 a         1256 c         1443 g         1785 t
ORIGIN      
        1 acttcaactc tgtgcttgta tcagtttcat gggagattat tgaaccctcg gaaggaaggt
       61 ttgacttctc ttcggtggat gagcttttaa ggatagcccg ggaaaacgat ctgaaggtgg
      121 gatttctctg gtttgcctca tggaagaacg gtttctcacc ctatgctcct tcgtgggtgc
      181 tgggtgatac aaggcgattt gaaagggtta aggatgaaaa gggccgcaat acacgcacgc
      241 tgagtccgct ttgcaatgct acaagagatg ctgatgccag ggcatttgct gccctcatgc
      301 gacatctggc acaggccgat tcgaaagaaa atacggtgat tgttgtccag attgaaaatg
      361 aggttggagt gctgaggcag acaagggatt tcagtgctgc ggcaaacaaa ctcattgcat
      421 cacaggtgcc tgaggagctt ttgagacatc tttctaaaaa ccgccagaca ctcgaaccgg
      481 agctcagaac tgcatgggaa aacaatgggg caaagacaaa aggtacatgg tacgaagttt
      541 ttggcgctgg tgattatacc gacatgtttt ttatggcatg gcaatatgca aggtatataa
      601 atgcagttgc tgaagcgggt aaaagggaat ataatattcc ggtgtatgtc aattgctgga
      661 tgtcgggttc aagacctaac attggaaagc cgggaaattt tccaagcgga ggtccggttc
      721 tttctgttct ggatatatgg aaagcaggag caccagcagt tgatattctt gcaccagaca
      781 tctatggtgc cgatttcaaa gaccagtgcc gtaactttca cagacacgac aacccgctgt
      841 ttgttcctga aacaagagct gtcgagggag ctgccacatg ggctttttca acacacgatg
      901 ccatatgctt ttcgccattc ggaatcgaca ccagacctga aaccatggct gcagaatata
      961 aaatgctttc tcagctaata ccggtcataa cacagtatca gggtacaggt ctgatgtcgg
     1021 ggttctatcg cgatggcagg gatacaacaa gaaactatga atttaaacta aatgatgatg
     1081 tgaagattat tgtcactctt caacaggctg cgcggaggga aacctcaccg actgccccgg
     1141 gaacaggcaa cgcccagcaa cgacagcaga cacccgaaca gggatcctat ggacttttta
     1201 taaaaacagg cgagagcgaa tttatggtgg caggtaataa tatctcagta actgctgtct
     1261 ctcttaatcc gaagaaggaa gtctggctta aagatgcctg ggaaggaaca ttagttgatg
     1321 gcgtatggaa acccaaagtt cttctcaacg gcgatgaggc aggttttctc agaagtgatg
     1381 atcctgtata ttcagtaaga gcttataagt caacacctcc ggaaccggca attattaagt
     1441 tcagagctgt cgtttacgac aggtaatgaa taagtcagca attttttata atagaataaa
     1501 aacaggtgcc gtaaataatt aaatttggaa cacagaaatc tgatgtcaaa attattatag
     1561 cagatgatta ctttcatttc aggcattctc atccttctgg caggatattt tgtttattcc
     1621 aggtttattg aaaaaattgc aggtgctgat gcctcgagag agactcctgc ctacaggatg
     1681 agagacggtg ttgattacat tccgatgccc tggtggagag tcttccttat acagtttctg
     1741 aatatcgcag ggctgggacc tatttttggt gccattgcag gtgctatgtg gggacctgtg
     1801 gcttatctgt ggataattct tggctctgtc tttgccggcg cagttcacga ttatttatcc
     1861 ggaatgcttt ctatttatca taacgggctc agtataactg agatagttgg aatttatctt
     1921 ggcaatggca ttaaacagtt tatgcggggc tttactgtcg tccttatggt tcttgtggga
     1981 gctgtattta ttgttggacc ggcaggaata ctgggaggaa tgacacaggg tacactttcg
     2041 gttactttct gggtgtgggt tgttttcttt tattacattg ttgccacaat gcttcccatt
     2101 gataaggtta tcgggagaat ctatccgttt tttgggtttg ctcttgtttt catggcattt
     2161 ggacttatta cagcattatt tattaacgga tataaaccac cggagcttac tatttcgaca
     2221 ctccggaact ttcatgttga ttccggaaaa tttcctgttt tcccgatgat gttcataaca
     2281 attgcctgcg gtgcaatatc cggatttcat tcaacacaat cacctcttat ggcaagatgt
     2341 attacaaatg aaaaactggg tagaagagtt ttctatgggg caatgatttc tgaagctatt
     2401 gttgcaatga tatgggcaac tatcggaatg agtttctggg gtggagtgaa ggaactgaac
     2461 gacataatga ctgccaataa aggcaatgct gctgtgctgg tgaatgaagt gtctaattct
     2521 cttctgggaa agttcggtgg catacttgca cttttaggag tagtagctgc tcccataaca
     2581 tctggtgaca cagccttccg cagtgcacgc ctcatagttg ccgattttct gaattttaaa
     2641 caggggccaa taaaaaaccg tttgattatc agcattccac tctttgcaat aggttttttg
     2701 attacaaaaa tggatttcag cattatctgg cgctactttg cgtggtgcaa tcaaaccctt
     2761 gcaatgatag tactatgggc agaaactgta tatctggtta gagcaggcaa aatttacgga
     2821 ataacacttg taccggcact ttttatgacc gccgtttcag caacctatat acttattgca
     2881 cctgagggtt tcagactggc aggcaatata gcttaccctg caggtgcagc agcaacactc
     2941 gctgccttga caggatttgt tgcatatttg tacaggagga gaactaaata aaagtaaact
     3001 attgccacgg attttgtctg gcagattgga aggatgacgt aaaaaagaag atagatatat
     3061 ttatttatgc ttaaataccg aatagcatat ttttttaatt tttcaaatat gaaaaccaga
     3121 agaaaattta ttaaagaaac agcagccgga gctatggcag caatgagcat tccaaaagtt
     3181 ttgtacgaat ttcctgctgg agcaggacag aagaaaataa aactggaaaa gaacaatgtc
     3241 attctttttc agggcgactc aattaccgat gcaggcagaa acaaacagaa tgaaagcttc
     3301 aataatccat catcattagg ttcgggttat gcttttctgg ctactgctgc tctacttgaa
     3361 agttttcctt cacttaatct gaaggtatat aacaggggta tttcgggtaa caaagtcttt
     3421 cagcttgccg aacgctggga taaagattgt cttgacctaa aacccgatgt gttgagtatc
     3481 ctgataggtg tcaacgatat ctggcatact ctgaacggac aatataacgg tacggttgaa
     3541 aaataccgca acgattatat tgctcttctt gaacgcacca gaaaagctct tccttctgtc
     3601 aaactgataa tctgcgaacc ctttgcagta cccggagtta aagccgttga cgacagatgg
     3661 taccctgaat tttatgaata ccagaagtct gcccgggaga ttgcagaacg cttcggggca
     3721 atatttgttc ccttccagaa agtttttgat gaggcacaga aacgcgcccc gggttcatac
     3781 tggaccggcg acggtgttca cccctcactt cccggagcac aactgatggc taaagcatgg
     3841 ctcaagacgg ttctttaatt tgatttaatt tccatgctgc caaaaagata tattatttca
     3901 tatcgctggc aggcatttgc ctttctgacc atctgcttgc tttacgggaa tatttccttc
     3961 tcccaggagg aatataatgt gcttaaagag agctggttaa aatatactga tgcacagaat
     4021 tttctttatc atcatctcct ttccgatgct gtttttcttc ttgaaaaaag aaaagaaagg
     4081 acagattcac tggcaacact tttcagctgg cagtcgcgtc agaaagaaat cagagaaata
     4141 cttcaaagaa ttaccgggcc atttcccgaa aaaactccct tgaatgcaag aattgtaaga
     4201 acaattgaga agcctggttt cagaatagaa catatcattt ttgagtcaca gccggggttc
     4261 tatgtgacat cttcacttta tattcctgcg gggattggaa aaggtcagaa actgcctgct
     4321 gtaatttatt gcagcggcca ttcagctgaa ggatacagaa gcgcagtata tcaacatgtt
     4381 atacagaatc ttgtaaagaa aggatttatt gtatttgctt ttgacccggt agggcagggt
     4441 gaacggcttg aatatcctga cccggcaacg ggcaaatccc tggttggtgg acccacacat
     4501 gagcattctt atcctggctc acagacactc ctgaccggta cctcgcaggc tgcatacatg
     4561 atatgggacg gaataagagc agttgattac ctgcttaccc gcagggaagt cgatccatct
     4621 cgcattggaa tcacaggacg ctcaggcgga ggaacacaat cagcatatat agctgcaatg
     4681 gacgacagaa tctttgctgc tgcccctgaa aattatttaa caaactacac ccggctgctt
     4741 gagtcaatcg gtccccagga tgcagaacaa aatattccga agtttattgc atctggcctt
     4801 gaccatgccg actttcttat tgtaagagct cccaaacctg ccatgatgat cacaacaacc
     4861 agagatatgt tcagtattca gggcgctgtg gagactgaaa aagaaataac ccgtgtctac
     4921 agcgcatata accagccagg aaatttctcg agggttgaag atgacacaac tcatgcttct
     4981 actgtaaaaa accgccaggc actttatacc ttcttcggga agcatctgaa caatccatgc
     5041 aataacagcg atgaggaaac aatacgtcta tcacctgaag aattgagggt gacatcaaag
     5101 ggtcaggttt catttgaatc aggagcagaa acagtccatt cccttaatct aaagagagct
     5161 atagcgcttg agaaacgtct tgatgactcc agaaacggtg aaataaagca tttcactgat
     5221 gcagtcgatg cggcaaaaaa gctctcaggt ttcattgaac cggaaagcgt tggagatcct
     5281 gtatttgcag gacgtttcag aaaggatggg tattgcgtgg aaaagtactt tataagaggt
     5341 gaaggtgatt atgtgatacc ttttcttctt tttgtgcctg ataaacagac cagtaaatat
     5401 ctgatatatc ttcatccgga agggaagtca gcagaagcag gggcagggaa agagattgaa
     5461 agcattgttt tacatgggat aacagttctg gctcctgacc tgcctggcac gggtgaaaca
     5521 ggtccgggtt catttcgcgg tgatgcatac tttgccggcg tttcacataa tcttctctat
     5581 gccgctttac ttacaggaag aagcataacg ggtatttatg caggtgatat tatacgtctt
     5641 gcaaagttta ttgaaaagag agatgaacat gcagatataa aaggctttgc taaaggatat
     5701 ctgtcaccag cacttcttca tgcagcagcc ttttcccatt tattgaaagg catcgccctt
     5761 gttaatcctt acatctcata cttttctctg gtaaaagaaa ggatgtacaa cccgcttttt
     5821 atgctaacag ccgtaccggg tgcccttaaa gaatatgatc ttcctgacct tgccggagct
     5881 cttgctcccc gaaaacttct gattacagga gctaccgacg gtaaaggttc cagtgaggat
     5941 agtgaagtaa tcaataaagg cctttcggta ataaaagctg cttattcagt aaataatgct
     6001 ggtgataaat taaggatata cccaatcact gataaagtgc atataattaa taatttgatt
     6061 gattggctga tgaaagaata attgtacaga acaataacat ggatatgtta aaaaaagtaa
     6121 aattccatct tttttttatg tgaaaaaatt tcttttaacg attttttata atttttgaga
     6181 ttaaatattt ctgaacacca aataaaatgg cttttaagcg aacccggaag agacgcgatt
     6241 ttttaagaaa cgcaaccctt ggcacaatag gattaacatt tgcttcgggt acagctttcc
     6301 ggaattacaa aacaggttct gaagtaaaaa aggtg
//
LOCUS       DAOSNB010000149         6326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12425_length_6326_cov_3.738957, whole genome shotgun sequence.
ACCESSION   DAOSNB010000149 DAOSNB010000000
VERSION     DAOSNB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12425_length_6326_cov_3.738957"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..444)
                     /locus_tag="PLG42_06915"
     CDS             complement(154..444)
                     /locus_tag="PLG42_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOU30976.1"
                     /translation="MNIFVARLSSKTRSEDLERLFSKFGEVTSSKVIVDKQTGFSKRY
                     GFVEMPDEQAAYEAIEKLNNTELDGSQIVVKMSVPTHRKKTRRRRNEPGNAK"
     gene            complement(581..2002)
                     /locus_tag="PLG42_06920"
     CDS             complement(581..2002)
                     /locus_tag="PLG42_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOU30977.1"
                     /translation="MSHTFTSGILEITYIVSSVMFVFGLKMLSHPDTARRGNIVAAIG
                     MMLAILATLLFHEKDGKHIGNIGWIVTAMALGTVIGWVIAKRVKMTAMPQLVSFFNGM
                     GGAAAALISMMEFPHVSPELIARSGMANGHVLAILLGLMIGTISWAGSMIAFGKLDGW
                     IGDLRIKALRYVNVTLLAVLIGFIVFIMTRRVAASAELMPYIYIMFGAAVVYGVLFVM
                     PIGGADMPVVISLLNSFTGVAAAMGGFLYNNQVMLTGGILVGSAGTILTVLMCRAMNR
                     SLLNVIAGVFGGGPAGAQTAGTIKEITLADAAVLLSYSKKVVIVPGYGLAVAQAQHIC
                     HELDKLLEDKGVEVKYAIHPVAGRMPGHMNVLLAEADVPYDKLVESDEINPELPETDV
                     VVVIGANDVVNPSAEDDPSSPIYGMPIVRVREAKNIIVMKRGMGKGYAAIENPLFYHD
                     KTRMLFGDAKVTLQNLVAEIKNL"
     gene            complement(2025..2330)
                     /locus_tag="PLG42_06925"
     CDS             complement(2025..2330)
                     /locus_tag="PLG42_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOU30978.1"
                     /translation="MEKVFEFFLVHREAIFLVILSIFLGIEVISHVPAVLHTPLMSGA
                     NAIHGVVIIGAIIVMGHAETTGSMVLGFLAVVLGTLNVVGGFVVTDRMLEMFKKRKK"
     gene            complement(2341..>3316)
                     /locus_tag="PLG42_06930"
     CDS             complement(2341..>3316)
                     /locus_tag="PLG42_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOU30979.1"
                     /translation="GATAGDRNEIIGKSDLLVSVNPPIDDKITDFKNGQILCCIVNPV
                     ENREWLEKARVQGLTVLALDLIPRTTRAQAMDILSSMATVAGYKAVIHAASLLPKFFP
                     MFMSAAGTIKPAKVLVLGAGVAGLQALAIARKLGAVVEVFDVRAAVKDEVKSLGGKFI
                     EVEGAVEDASAGGYAVEQTEEYKRKQQEMIQKHAAAADVIIATAQIPGKRAPVLILKD
                     TVMKMKPGSVIIDLAASTGGNCELTQNGKMIEVNGVKIIGKSDYPSDMPTDASKMFGA
                     NIVNLLKILVDREGKPVLDFSDEIVTGTTAVHGGQYISQRIKQLLNLQ"
     assembly_gap    3317..3326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3682..5844)
                     /locus_tag="PLG42_06935"
     CDS             complement(3682..5844)
                     /locus_tag="PLG42_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU30980.1"
                     /translation="MKYIFKLRFSLLLIIISALTINLFAQFQRGGSGQHRVTGWVDDT
                     HFIFQTLDRDNMPITLSVDIRTGKSTPYTEPESPRDLFIKFLPRGVNLGLSDVLSPDS
                     KSAVFIKNNDLYFFRIGDRELRRLTNDNAEEVNVRFSPDGLKIAYTKNKDLYAYDLVN
                     FKEIRLTFDATDRIYNGYASWVYMEEILGRASRYAAFWWSPDSRHIAFLRTDETEVPV
                     FTLNRLDEPDGIHGKLEVTPYPKAGDPNPKVKMGIADITNSSIVWVKTDENIDQYIAW
                     PFWKPDGSKLAIQVLNRDQNDMKFILADINTGEYSQIYSETRKTWVDFYEDIYVMKNG
                     TGFIVRSYRTDWENLYYYGWDGRLISQITNVDWRVTGIVRVDEDTKKVFFTGTGPEST
                     DSHFFVASLDGKSMIQITKGPGTHSVSISPKGTYFIDTWNNINTPGAIVAYDSKGKQI
                     REIHKFEQPEFDPAKHARSEMVKIPTSDGLFLMPAIITYPVNFDETKKYPVVFTIYGG
                     PDSKNVNNRWQGYNPSWYAQNGIITFSVDHRGSGHFGKKGLDYMHRNLGKWEILDYAD
                     AVKWLRQKPYVDPERMGITGSSYGGYMTCLALTKGAEYWTHGFAGSSVTDWKLYDDIY
                     TERYMDTPQDNPEGYREGSVLTFVKNYKGKLYMTHGDMDDNVHMQNSIWLISKLQDEG
                     KTFQFMLYPNGRHGWGGAKAVHSSNERNNFWLSVFFGK"
     gene            6116..>6326
                     /locus_tag="PLG42_06940"
     CDS             6116..>6326
                     /locus_tag="PLG42_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU30981.1"
                     /translation="MSLISVVVVDDEKPACDRLSKLLARFKEVELFGSFTDSLKAVEF
                     IKKNNPDIVFLDVELENGISAFDIIS"
BASE COUNT         1738 a         1441 c         1209 g         1928 t
ORIGIN      
        1 ataactcagc agatatacca ccgcgttctc ctgtaaaaag tacaggagag cctgaaatga
       61 ttacacttgt tccttacggc tctgcaaaac tcaggataac ggaatttccc ttaatagatg
      121 tcgggtttat ggataaacaa tgaatactat tgtttatttc gcgtttcctg gctcatttct
      181 gcggcgtctt gtttttttcc tgtgagttgg aactgacatt ttaactacaa tctgactgcc
      241 gtcgagttca gtattgttaa gtttctcaat tgcttcgtaa gcagcctgtt catcgggcat
      301 ctcaacgaag ccgtagcgtt tggagaagcc agtttgtttg tcgacgataa cttttgagga
      361 agttacttcg ccaaacttgc tgaagagtcg ctcaaggtcc tcgctgcgag tctttgagct
      421 gagtctggct acgaaaatgt tcatctgaaa aagtttttgt taataaaaag tataacttcc
      481 cgttattgct ttcatacagc aagcggtaga gtcacttata caaatttaag tacttttttg
      541 aaaatcagca aacaataaaa attattaaat aatggcctgc ttaaaggttt ttaatttccg
      601 ccacaaggtt ctgtagggtg acctttgcat ctccaaagag cattcttgtt ttatcgtgat
      661 aaaacagagg gttttcaatt gcagcgtaac ctttccccat tcctcgtttc attacgataa
      721 tattttttgc ctcgcggact cttacaattg gcattccgta aattggactg gaaggatcat
      781 cttctgcaga cgggtttaca acatcattag ctccaataac aaccacaaca tctgtttcag
      841 ggagttcggg attgatttca tcactttcta ccagcttgtc atatggtacg tctgcttcag
      901 ccagaagcac attcatatgt ccgggcattc taccagcaac aggatgaata gcatacttaa
      961 cctcaacgcc cttatcttcc agaagtttat ccagttcatg gcatatatgc tgtgcctgcg
     1021 caactgcgag cccataaccc ggaacgatga caaccttttt tgaatagcta agcagaacgg
     1081 ctgcatcggc aagggttatt tccttaatgg ttcctgcagt ctgggctccg gccggccctc
     1141 caccgaatac tccggcaata acattaagca acgacctgtt cattgccctg cacataagta
     1201 ctgtaaggat tgttcccgcc gatcccacga ggataccacc ggtaagcata acctgattgt
     1261 tgtaaagaaa gcctcccata gcagcggcaa cgccggtaaa agagttaaga agtgaaataa
     1321 caacaggcat gtcagctcca ccgataggca taacaaacag cacaccgtaa accactgctg
     1381 caccaaacat tatataaatg tatggcatca gttcagcact cgctgcaacc ctgcgtgtca
     1441 taatgaaaac tatgaatcca atcaggacag caaggagggt aacgttcaca tatcttaatg
     1501 cttttattct caggtctcct atccatccat ccaactttcc gaatgcaatc atgcttcctg
     1561 cccaggagat agttcctatc atgaggccta gcaaaatagc aagcacgtgc ccgtttgcca
     1621 taccgctgcg tgctatcagt tcaggactta catgaggaaa ttccatcata gaaataagtg
     1681 ccgctgccgc acctcccatt ccattgaaaa acgatacaag ctgaggcata gcagtcatct
     1741 taaccctctt cgctatgacc catcctataa ctgttccgag agccatcgct gtaacaatcc
     1801 atccgatgtt gccaatatgc ttcccatcct tttcatggaa cagaagagtt gccagaatgg
     1861 caagcatcat tccaatggcg gcaacaatat ttcctctgcg ggctgtatcg ggatggctca
     1921 gcatcttcag cccaaaaaca aacattacag atgaaacaat atatgtgatc tctaaaattc
     1981 ctgaagtgaa tgtatgactc atcagtaaaa aaatttgtta gtgattattt tttcctcttc
     2041 ttgaacattt caagcatacg gtcggttacc acaaaacctc ccactacgtt taaagtacca
     2101 agaaccactg caagaaaccc gagtaccatg gagcctgtag tttcagcatg tcccatcact
     2161 atgatggctc cgattatcac aacaccatga atggcatttg cacccgacat caatggtgta
     2221 tgaagcacag caggaacatg acttattacc tcaattccaa gaaagattga aagaatcaca
     2281 agaaatattg cttccctgtg cacaagaaag aactcaaata ccttttccat cagctgttaa
     2341 ttattgaaga tttagtaatt gttttattct ctggctaata tattgaccgc catgcacagc
     2401 ggttgttccc gttacaattt catcagaaaa atccagaaca ggtttccctt ccctgtctac
     2461 gagaatctta agcaggttaa caatatttgc cccgaacatt ttacttgcat ccgtcggcat
     2521 gtcggaagga tagtcggatt tacctataat ttttactccg ttaacttcaa tcattttccc
     2581 gttttgagtc agttcacagt ttccgcccgt tgatgctgca aggtcaatta ttactgagcc
     2641 tggcttcatt ttcatcactg tatctttaag tatcagaacg ggagctcttt tgccgggtat
     2701 ctgggctgta gctataatta catcagcggc agctgcatgt ttctgaatca tctcctgctg
     2761 ttttctttta tactcttctg tttgttcgac agcatatccg ccagccgatg catcctctac
     2821 tgcaccctca acctcaataa atttacctcc cagacttttt acctcatcct taactgcagc
     2881 tcttacgtca aaaacttcaa ccacggctcc gagctttctg gctattgcca gtgcctgcaa
     2941 tcctgccaca cctgcaccaa gtacaagcac tttagcaggt tttatggtac ctgcagccga
     3001 catgaacatt ggaaaaaact ttggcagtaa tgaggcagca tgtattactg ccttataacc
     3061 tgcaactgtc gccattgacg aaagtatgtc cattgcctgt gcccttgtgg tgcggggaat
     3121 aagatcaagg gcaagaacag taagcccctg cactcttgct ttttcaagcc actctctgtt
     3181 ttcaacagga tttacaatac aacagagtat ctgaccattt ttgaaatccg ttatcttatc
     3241 atcgatggga ggatttacag aaacaaggag atcagacttc ccgattattt cattcctgtc
     3301 gcctgcagta gctcctnnnn nnnnnngcag gtgtctcacg gttatggtct tggtagcggt
     3361 aatcgccatc aaaagcaaaa gccctctctc ctgcccctct ttccaccata acttcaatac
     3421 caagctttcg aactgcctgt acttccccgg gaagcatcgc caccctgttt tcgttatttt
     3481 tttcttttaa aataccaatg atcataaaat atttttattt ttaaaagcca caaatttaac
     3541 aatgtttaga atattagctt aaaaaaattt caattctaaa taccatcaca tccattaatt
     3601 aataatcaac attcggaaca tctgcgtctc tgaagcctta ttcatggaga tcagctctgc
     3661 tcctaccagt tcattactcc ttcattttcc gaagaaaaca gaaagccaga aattgtttct
     3721 ttcgtttgaa gagtgaacag cctttgctcc tccccaacca tggcgtccgt ttggataaag
     3781 cataaactga aaagttttgc cctcatcctg aagttttgaa atcagccata ttgaattttg
     3841 catatggaca ttatcgtcca tgtcgccatg agtcatgtaa agtttccctt tgtaattctt
     3901 tacaaatgtc agcacagagc cttctctgta tccttcggga ttatcctgtg gtgtatccat
     3961 gtatcgttct gtatagatat catcataaag tttccaatcg gtaactgatg aaccggcaaa
     4021 gccgtgtgtc cagtattcgg cgcctttggt tagtgccaga caggtcatat aacctccgta
     4081 tgagctacct gtgatgccca ttctttcagg atcgacatac ggtttctgtc ttagccattt
     4141 aacggcatca gcataatcga gtatctccca ttttcccaga ttacggtgca tatagtcaag
     4201 accctttttc ccaaaatgac ctgagccgcg atgatctacc gaaaaagtaa tgataccgtt
     4261 ctgggcatac cacgatggat tgtatccctg ccatctgttg ttgacatttt tagaatcagg
     4321 tcctccataa attgtaaaga ccacagggta tttttttgtt tcgtcaaaat ttacagggta
     4381 tgttattatt gccggcatca gaaatagtcc atctgatgta ggaattttga ccatttctga
     4441 ccttgcgtgt tttgcagggt caaattcggg ctgttcaaac ttgtgtatct cccttatctg
     4501 ttttcctttg ctgtcgtatg caactatagc gcccggagtg ttaatattat tccatgtatc
     4561 aatgaaatat gttccttttg gcgaaatact taccgaatgt gttccaggtc ccttggtaat
     4621 ctgaatcatg ctcttcccat ccagagaggc tacaaaaaaa tggctatcgg tcgattccgg
     4681 gcctgtaccc gtaaaaaata ccttcttcgt atcctcatca acgcgcacaa tacctgtaac
     4741 tctccagtcg acatttgtaa tctgcgagat cagtctgcca tcccatccgt agtaataaag
     4801 gttttcccag tcggtgcggt aacttctcac gatgaagcct gttccatttt tcatcacata
     4861 aatatcttca tagaagtcga cccaggtttt acgggtttca gaatatatct gagaatattc
     4921 accggtattt atatcggcaa gaatgaactt catatcgttc tggtcgcggt taagcacctg
     4981 tattgcaagt tttgagccgt cgggtttcca gaaaggccag gctatgtact ggtcaatgtt
     5041 ttcatcggtt tttacccata ctattgaaga attggtaatg tcggcgatgc ccatttttac
     5101 tttcgggtta ggatctcctg ctttagggta aggggttact tcaagtttac cgtgtattcc
     5161 atctggttca tcaaggcggt taagggtaaa gactggtact tcggtctcgt cggtgcgaag
     5221 gaaagcaata tggcggctgt cgggcgacca ccagaaagca gcataacggc ttgccctgcc
     5281 aagtatctct tccatgtaaa cccacgaagc ataaccatta taaatacggt cggtggcatc
     5341 aaacgtcaga cgtatctctt taaagtttac cagatcgtaa gcatacaggt ctttgttttt
     5401 ggtatatgca attttcaatc cgtcgggcga aaaccttacg ttgacctcct ctgcattgtc
     5461 gtttgtaaga cgccgcagtt ccctgtcacc tattctgaag aagtatagat cgttattttt
     5521 aatgaatact gcactcttag agtctgggct aagtacatct gaaaggccca ggtttacacc
     5581 acggggaaga aattttataa ataaatctct cggagattct ggctctgtat atggtgtgct
     5641 tttgcctgta cggatatcca cgctcagtgt aattggcatg ttatccctgt cgagagtctg
     5701 aaagatgaaa tgagtgtcgt caacccatcc cgttactcgg tgttggccac ttccgcctcg
     5761 ctgaaactgg gcaaagagat taattgtaag ggctgaaata attatcagca gtaacgaaaa
     5821 ccttagtttg aatatatact tcatggcgat aatgagagtt taataagtgt ttcagaaaat
     5881 gcaaaaatag ttatttcggt gaattggttt ccaataatat aatcaatgat atacgaacaa
     5941 tttaaataaa attgattatc agatatttag tcgtttctaa tgtttattca caactacttt
     6001 atggttcaaa aaatttcatt gaattctgat ttttttttat ttttgaaaag gcaccatgtt
     6061 tgcagtcatt atgaaatgcg gggaggaatt tattaataga taattttttt attttatgag
     6121 tttaatttca gtcgtcgtgg ttgacgatga gaaaccagcc tgcgaccggt tgtcaaaact
     6181 gctggcaagg tttaaagagg ttgagctgtt cggctcattt actgattccc tgaaagcagt
     6241 agaatttatt aaaaagaaca atcccgatat tgtcttcctc gatgttgagc tcgaaaacgg
     6301 aatatctgca tttgatataa tctcag
//
LOCUS       DAOSNB010000150         6246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12627_length_6246_cov_5.359554, whole genome shotgun sequence.
ACCESSION   DAOSNB010000150 DAOSNB010000000
VERSION     DAOSNB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12627_length_6246_cov_5.359554"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLG42_06945"
     CDS             complement(<1..1188)
                     /locus_tag="PLG42_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOU30982.1"
                     /translation="MKPSKFIIRHLCAALFLAVIVIPVESQSITGDWYGSISVQGISL
                     RINLHVTRSENGYTSTWDSPDQGAIGIPSTTTTFTYPDFSFSHSQAGFRYSGKVTPDY
                     SAIEGTLFQGGQKFELTFTRRPVAAAPGSQAALKEKYEKQEVYITMRDGIRLFTSIYI
                     PKNKTVLHPILLNRTPYNIEPGGPDAFNYFMQVYSRYAESEYIMVFQDVRGKYMSEGV
                     FEDIRPVIPVKTSDKDIDETTDTWDTVDWLVKNVPGNNGRVGIFGISYPGFYSTMGAI
                     NAHPAVKAVSPQAPVTAWFIGDDFHHNGAFFLLDCFSFFYSNGHQHRTPSRRGFPSFK
                     WPVPDNYEFFLSLGPIRNISPKYFGDSVKFWNDAFAHPDYDDFWKARDPRQYLKNIKP
                     AVMT"
     gene            complement(1239..1670)
                     /locus_tag="PLG42_06950"
     CDS             complement(1239..1670)
                     /locus_tag="PLG42_06950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOU30983.1"
                     /translation="MNNHNINMHSNKYIFCLDFRVRDYECDLQGIVNNANYLHYLEHT
                     RHEFLLSKGISFSDLHNEGTDLIIVRIEIDYKFPLRSKDTFKVCLNVRKEGNVRIAFE
                     QDIFRLPDYKHIVHAVVMGAATKNGRPVSPEFVLKKLKLPD"
     gene            complement(1701..2675)
                     /locus_tag="PLG42_06955"
     CDS             complement(1701..2675)
                     /locus_tag="PLG42_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOU30984.1"
                     /translation="MSALSEIRRPVEKEMAEFERFFNRSFKSEVPLLKIILNYIFRRK
                     GKQMRPLLVFLSARLNGEINESAYVAATLIELLHTASLVHDDVVDDAMERRGALSVNA
                     LWNSKIAVLVGDYMLATGLLVSIENNRYDMLEIVSEAVKSMAQGELLQLQKARKLNIK
                     EDDYFKIISSKTAALIAACTACGTISVTKDPDIVQMMKELGENIGIAFQIRDDILDYE
                     RNGLTGKAPGNDIKERKITLPLIYALEKSPVNEKKHMINIVKKKKKSRDEINQVIKYV
                     EDTGGIEYAETKMNQYRDKALAILDYYPDSEVKNSMKEFIHYTTSRNS"
     gene            complement(2804..3421)
                     /locus_tag="PLG42_06960"
     CDS             complement(2804..3421)
                     /locus_tag="PLG42_06960"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOU30985.1"
                     /translation="MKLSTGHKAILFILLILLVDQALKIWVKTHMTIGQEIPIFGRYG
                     MLHFIENNGMAFGMELGGKGGKILLSIFRIIAIAGISWYLSILIKKNANWSLIMAVSA
                     IVAGASGNFIDSAFYGIIFSESTYDKVAVLFPPEGGYSSLLLGKVVDMFYFPVINTTW
                     PDWSPIKPGQQFIFFRPVFNISDAAITCGVFSIIIFQKKMFKNIS"
     gene            3522..4778
                     /locus_tag="PLG42_06965"
     CDS             3522..4778
                     /locus_tag="PLG42_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU30986.1"
                     /translation="MTSKEIILAALDHKETGRIPVDFGSTSVTGIHVRAVENLRNYYG
                     LEKKPVKVIEPFQMLGEIDEDLAEILGTDVIGLTSRKNMFGIPQDEGWKEFRTFWGQV
                     VLLPEKFNTTFDEDGALLIYPEGDTSVPPCAKMPKAGYFFDAIIRQEPIDENNLNVED
                     NLEEFSLIQEEDIAWWKKQFDLARKSNKAVVANFGGTAIGDIALVPGLNLKCPKGIRD
                     VAEWYISTVARQDYLHKIFEKQTDIALQNLSKLQKAGGDAVDVVFICGTDFGTQTSTF
                     CSGEVFDRLYAPYYKKMNDWIHSNTRWKTFKHSCGAVEPFIRKFIECGFDILNPVQIN
                     AAGMNPVQLKKKYGKDIVFWGGGVDTQKVLAFGTPQEVRRQVIENCRIFGEGGGFVFN
                     TVHNIQANVPVENIVSMVETLKELNS"
     gene            complement(4779..5612)
                     /locus_tag="PLG42_06970"
     CDS             complement(4779..5612)
                     /locus_tag="PLG42_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30987.1"
                     /translation="MKKLFVAFLVLVLVPVFQSCEKKGNPPVIPPFETMAIDFSNFST
                     ETKSFQQFDLPKSTVAVPNLNWSIAATVAGVWNTLLFVNLAVPVAAFKKALETKPSYL
                     DNKKWQWKYSVDVLSVKYTARLTGQISSTGVKWEMYISREGAGGFAEFLWFEGTSAPD
                     GKSGQWVLYHNWQEQVPVLQIDWKITGTEVNSVKYTYIKTGSAMKDSYIEYGLTTGDL
                     NAYYNVHYYESTSMLKFIDVQIKWSTTGHHGRIKAPDYFGDDLWHCWDSNYNDTECPS
                     R"
     gene            5838..>6246
                     /locus_tag="PLG42_06975"
     CDS             5838..>6246
                     /locus_tag="PLG42_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30988.1"
                     /translation="MNRRKFFKSAGAGASLFLLTGNALLTGKEIIKSETMEQMSTSKI
                     LFPALPYAYDALEPWIDARTMEIHYDKHHRAYYNNFVAAIKGTESENLTAEEILKNVS
                     RYPVAVRNQGGGYFNHVLFWENMAKGLSTPSSEM"
BASE COUNT         1928 a         1154 c         1233 g         1931 t
ORIGIN      
        1 tgtcattact gcaggtttaa tatttttaag atactgcctg ggatccctag ctttccagaa
       61 atcatcataa tcaggatgtg caaatgcatc attccagaat ttgacgctgt cgccaaaata
      121 ttttggcgat atatttctta tagggcctag tgaaaggaaa aactcataat tgtcgggaac
      181 aggccatttg aatgatggaa atcctctgcg gcttggagtg cggtgctggt ggccattgct
      241 gtaaaagaaa ctgaaacagt cgagaaggaa aaatgctccg ttgtggtgaa aatcgtcacc
      301 aataaaccat gcagtaacag gtgcctgggg tgaaactgct tttaccgcag gatgggcatt
      361 tatagctccc atggtggaat aaaaaccagg gtacgaaata ccgaatattc caaccctgcc
      421 attgttacct ggaacatttt taacaagcca gtcaaccgta tcccatgtat cagtagtttc
      481 atcaatgtct ttgtcagagg ttttgactgg tataaccggt ctgatatcct caaaaacacc
      541 ttcactcata tatttacccc ttacatcctg aaaaaccata atgtattctg attcagcata
      601 tcttgagtaa acctgcataa agtaattaaa ggcatcaggt cctcctggtt caatattgta
      661 aggtgtccgg tttaacaata tcggatgcag tacggtttta ttcttaggta tataaattga
      721 tgtaaataat cgtattccat cgcgcattgt tatatagact tcctgttttt catatttttc
      781 tttcaaagct gcctgactgc caggagcagc agcaacagga cgtcgtgtga atgtaagttc
      841 aaacttttgt cctccctgaa atagagtacc ttcaatagcc gaatagtctg gcgttacttt
      901 cccggagtat ctgaatcctg cctgtgaatg agagaatgaa aaatcagggt aagtgaaagt
      961 tgtagtagtt gagggaatac ctattgcacc ctgatccggg ctgtcccagg ttgaagtata
     1021 gccattctct gaacgagtta catgaaggtt aatacggagt gaaataccct gtactgaaat
     1081 tgatccatac cagtcacctg tgattgactg gctttctacc ggaattacga taactgctaa
     1141 aaataatgca gcacataaat gtctgataat aaattttgaa ggtttcattt gggtatattt
     1201 taatgatttc aatttaatct atgtacataa ataatttttc aatctggtag cttgagcttt
     1261 tttagtacaa attcagggga gacgggtctt ccgttttttg ttgcagcacc catcacaact
     1321 gcgtgaacaa tgtgtttgta atcaggaagt ctgaaaatat cctgttcgaa tgcaattcga
     1381 acatttcctt cctttcttac attaaggcat accttgaaag tatccttgct tcttagaggg
     1441 aacttgtaat caatttcaat gcggacaata ataagatctg taccttcatt gtgaagatct
     1501 gaaaaactta ttcctttcga cagaagaaac tcatgacgtg tgtgttcaag ataatgaaga
     1561 tagtttgcat tattaacaat tccctgaagg tcacattcat aatcgcgcac tctgaaatca
     1621 aggcagaaaa tatatttatt ggaatgcatg ttgatattat ggttgttcaa agtttgagtg
     1681 ttttaataat gttagatttt tcagctgtta cgtgatgttg tataatgaat aaattctttc
     1741 attgagtttt tcacctcgga atctggataa tagtcaagta ttgccagagc tttatcacgg
     1801 tactggttca ttttggtttc agcatattca attccaccgg tatcttcgac gtatttaatt
     1861 acctggttta tctcatccct gctttttttc tttttcttaa cgatattgat catgtgtttc
     1921 ttttcattaa ccggtgattt ttccagagca taaatcaggg gtagagttat tttgcgttcc
     1981 ttgatatcat ttccgggtgc ttttcctgtt aatccatttc tttcataatc gagtatgtca
     2041 tcccggatct ggaaagctat tccaatgttt tcaccgagtt ctttcatcat ctggacaata
     2101 tcgggatcct ttgtaacaga tatagtgccg caggcagtgc aggctgcaat gagtgcagct
     2161 gtttttgaac tgataatttt gaaataatca tcctctttta tgttcagttt tcttgctttc
     2221 tggagttgaa gcagttctcc ctgggccatt gatttaacag cttcggaaac aatttccagc
     2281 atgtcatagc ggttattctc gatactcaca agaagtcctg tcgccagcat atagtcaccc
     2341 acgagaacag caatttttga attccataat gcatttaccg acaatgcacc ccttctttcc
     2401 attgcatcat ctacaacatc atcatgtaca agggatgccg tgtgaagcag ttcaatcagt
     2461 gttgcagcaa catatgcaga ttcatttatc tccccgttaa gccgtgcaga gaggaataca
     2521 agaagtggcc tcatctgctt gccttttctt ctgaaaatgt aattcagtat tattttcagc
     2581 aatggcactt cgcttttaaa ggacctgttg aaaaaccgtt caaattcagc catctctttt
     2641 tctacaggtc gtctaatttc tgaaagcgca gacatagata aaaaacaatg aataaatgat
     2701 tattaatttt caaacttacg aaaaataaat ggcagaatgt catttacttt gtaatatttc
     2761 ttatcgacgc aaggaaatta tgaaggaaag aaatttacag aaatcagcta atgtttttga
     2821 acattttttt ctggaaaata ataattgaaa agactccaca ggttattgca gcatcagaga
     2881 tattgaatac aggcctgaaa aatataaatt gctgacccgg ctttattggc gaccagtcgg
     2941 gccatgtagt atttattaca ggaaaataga acatatcaac gaccttacca agaagcaatg
     3001 atgaatatcc accttctgga gggaacaata ctgcaacttt atcataagta ctttcactaa
     3061 agataatgcc gtaaaaggcg ctgtcgatga aattgccgga agcgcctgcc actattgcac
     3121 ttactgccat aatcaggctc cagtttgcat tttttttgat aagtattgaa aggtaccagc
     3181 ttattccagc aatagctata attctgaaga tgctaagtaa aattttaccc cctttgcccc
     3241 cgagctccat gccgaatgcc ataccattat tttcgataaa atgcagcatt ccatatctgc
     3301 caaaaattgg tatttcctgg cctattgtca tatgtgtttt gacccatatt tttagcgcct
     3361 gatcaaccaa caggatgagg aggataaaca gaatagcttt atgacccgtc gatagtttca
     3421 ttctgaaact ggaaaataaa ttcatggcgc aaaataagca aaaaaagaat ttattaaaga
     3481 aaatattatc tttctacttt gaattaataa tttgaagaag tatgacttca aaagaaatta
     3541 ttcttgcagc ccttgaccat aaagaaacag gaagaatacc tgtagatttc ggaagtacca
     3601 gcgtaacagg tattcatgtc agggcagtgg aaaacctcag aaattattac ggacttgaaa
     3661 agaaaccggt aaaggtaatt gagccttttc aaatgctggg ggagattgat gaagatcttg
     3721 cagagatatt ggggacggat gttatcggac taacatcacg aaaaaacatg tttggaattc
     3781 cacaggatga agggtggaaa gaattcagaa cattctgggg acaggttgta ttattgccag
     3841 agaagtttaa tacaacattt gatgaagatg gtgctttgct gatttatccg gaaggagata
     3901 cttctgtgcc tccatgtgca aaaatgccaa aagcagggta tttttttgat gctattatcc
     3961 gtcaggaacc aatagatgag aataatctca atgttgagga taaccttgag gaatttagcc
     4021 tgatacagga agaagatatt gcatggtgga aaaaacaatt tgaccttgcc agaaaaagca
     4081 ataaagcagt agttgcaaat tttggtggaa ctgcaattgg agatattgca cttgttcccg
     4141 ggctgaatct caaatgccca aaaggtataa gagatgtagc agaatggtat atttctactg
     4201 ttgccaggca ggattatctg cataagattt ttgaaaaaca gacggatata gctttgcaaa
     4261 atctttcgaa gctgcaaaag gcgggaggcg atgctgttga tgttgtattt atctgcggca
     4321 ctgattttgg aactcagact tcaacatttt gcagcggtga ggttttcgat agactatatg
     4381 ctccctatta caaaaagatg aatgactgga tacacagcaa cacaagatgg aagactttca
     4441 aacattcatg tggtgcagtt gaacccttta taaggaaatt tattgaatgc gggttcgata
     4501 tacttaatcc ggtccagatt aatgcagcag gtatgaatcc agttcagctg aaaaagaaat
     4561 acggaaaaga tattgtattc tggggtggcg gtgttgatac ccagaaggtt cttgctttcg
     4621 gaacacctca ggaagtacgc agacaggtaa tagaaaactg tagaattttt ggagagggag
     4681 gaggttttgt ttttaataca gtacacaata tacaggccaa tgttccggtt gagaacattg
     4741 tttcgatggt ggaaaccctt aaagagctta acagttaact acctgctcgg gcactcagta
     4801 tcgttatagt tactatccca gcaatgccag aggtcgtcac cgaaatagtc gggagctttt
     4861 atcctgccgt gatgtccagt agtactccat ttaatttgta catcaataaa tttcagcatt
     4921 gaggtggatt catagtaatg aacattatag taggcattta aatcacctgt ggtaaggccg
     4981 tactcaatat agctgtcttt cattgcgctc ccggttttta tataagtata ttttactgaa
     5041 ttgacctcag tgcctgttat tttccagtcg atctgcagaa caggaacctg ttcctgccaa
     5101 ttatggtaca atacccactg cccgctttta ccatcaggtg cagaagtgcc ttcaaaccaa
     5161 aggaattcag caaaacctcc tgctccttca cgtgaaatat acatctccca tttgactcct
     5221 gttgaagaaa tctgtccggt taatcttgcg gtatatttta cggatagaac atcaacacta
     5281 tatttccatt gccacttttt gttatccagg tagctgggct tcgtttcaag ggctttttta
     5341 aatgccgcta ccggaaccgc cagattgacg aaaagaagag tattccagac tcctgctacg
     5401 gttgccgcta tgctccagtt gagattggga actgcaacag tacttttagg taaatcaaac
     5461 tgctggaaag attttgtctc tgttgagaaa ttgctgaaat caatagccat ggtctcaaaa
     5521 ggaggaatta ctggtggatt tcccttcttc tcacaactct ggaaaacggg aaccagaacc
     5581 agaaccagaa aagcaacaaa taactttttc attttccaat tcatttttat tataaaacgg
     5641 tgattccaaa taaaatattg caaaaacact caaaaaagta ttgccaaata attatttgat
     5701 attcaattta ataatatagt caaagtataa aaagttgtct aattttttct gcttgttttg
     5761 aacaaactta agtattgttt tgttattatc tgtgaattat ttaaattgta gatgtaaaat
     5821 tacaataaat aaaaataatg aacagaagaa aattttttaa atcagccgga gcaggtgcct
     5881 ctttgtttct tcttacaggg aatgcgcttt taaccggcaa agaaataatt aaatctgaaa
     5941 ctatggaaca gatgagtaca agtaaaattt tatttccggc attaccttat gcctatgatg
     6001 cactcgaacc ctggattgat gcccgtacaa tggaaataca ttatgataaa catcacaggg
     6061 cttattacaa caactttgtt gccgcaatca aagggacaga atctgaaaat ctcacggcgg
     6121 aagagattct gaaaaatgta tcacgatatc ctgttgcggt aaggaaccag ggtggcggat
     6181 attttaatca tgttcttttc tgggagaata tggcaaaagg attgtccaca ccatcatccg
     6241 aaatgg
//
LOCUS       DAOSNB010000151         6246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12628_length_6246_cov_3.937490, whole genome shotgun sequence.
ACCESSION   DAOSNB010000151 DAOSNB010000000
VERSION     DAOSNB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12628_length_6246_cov_3.937490"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..975)
                     /locus_tag="PLG42_06980"
     CDS             complement(154..975)
                     /locus_tag="PLG42_06980"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOU30989.1"
                     /translation="MDLNIRLSDGLLLRGYKWSPSGEAKASVIMVHGLGEHIMRYSDW
                     ATKFTSAGIMFQGVDLPGHGRSDGKRGHIKNYDQIDEMLDILLEESAKSYPERPVFIY
                     GHSMGGGIVLAYLIKRNPHIKGAIVTSPWIRLAFPPSGAKVTLARIMKSILPSLTQHT
                     GLVTEHLSHKQDEVDKYEKDPLVHGRISVSLFFSAVSSGEYIMKNAESINVPVLLLHG
                     AEDKITSPAASMEIASRSNKITFRLWEGGYHELHNEPFRDEVFSVIIDWIKSNTE"
     gene            1266..1769
                     /locus_tag="PLG42_06985"
     CDS             1266..1769
                     /locus_tag="PLG42_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU30990.1"
                     /translation="MTIHEFNNRLIELKGNLHRFALSLTYNREDALDLVQDTYLKAIA
                     NKDKFVDYTNLKAWVFTIMKNTFINNYRRGQKGNTIIDSTKDLYYINLPQDKGSISPE
                     SSYAAEEIEKAIDSLDDEYRLPFRMHVEGFKYEEIAEKLKLKTGTVKSRIFFARQKLM
                     KILKDYN"
     gene            complement(1783..2829)
                     /locus_tag="PLG42_06990"
     CDS             complement(1783..2829)
                     /locus_tag="PLG42_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU30991.1"
                     /translation="MKKTFKTVLILFLSLHSCNEPETTVTDIVRMDGSIERRIEMRGA
                     NAKFNIPDVQVPYDSTWSIRDSLEINKNGDTIRVRRAVKNFSNAEELNNYYSSDSSLN
                     KSTVRRVEFRKKFRWFDTHYSFSEIVEKRINHGYPVASFMPDDELKWFYLPEAEKTRL
                     LIGPDSLRYKVLDDSVKSHQEIWMWRSLISEWINTFNKLTEGKGIKGNTKEKEAILYS
                     MVRDNMNDFDSLWSQGVILKKLTGEKEAIEFKEEADSAMRVIEDLLGQRFSNYSVRIV
                     MPGKQVSSNSMLKDSTGFPLWQVKSEFFLTEPYIMKAESIVYNIWAWLITATFIILFA
                     GAIIFPVKKQKPAI"
     gene            2929..3339
                     /locus_tag="PLG42_06995"
     CDS             2929..3339
                     /locus_tag="PLG42_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOU30992.1"
                     /translation="MLQKVISLSTHSRNGLYDITSIVESVVKESGVRSGIASVYARGS
                     TCAIMIQENWDESVQTDVITLLNKLIPHGVWEHDRQDNNGDAHLKAGIVGPSETIPVV
                     EGKLGLSTWQNIFFCEFDGPRNRREIMVTVIGEK"
     gene            complement(3357..4295)
                     /gene="queG"
                     /locus_tag="PLG42_07000"
     CDS             complement(3357..4295)
                     /gene="queG"
                     /locus_tag="PLG42_07000"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOU30993.1"
                     /translation="MGNTGDISAAIKKKAYTLGFDLCGIARYRRLSEYEGPFTQWCRA
                     GMNDSMNYLSGSIARRIDPALFFPGVRSIVVTGMSYFTADIQTGMDVPIISRYALGTD
                     YHHVITGKLESLLSFIRTIVPGVQGKTSCDSSPVLEKPWAVEAGLGWQGKNSLVINET
                     KGSFFFIGILMLDIDLEYDEPVREDRCGSCEECINRCPTGAINTNRTIDARKCIANLT
                     IENRNPIDYGVAHLFERRVYGCDICQEVCPWNKNIPYHNHPELNASPEILSMTRQEWL
                     SLDREKFLKLFGKTPVARVGFERFKKNVEIILQYSA"
     gene            complement(4303..4623)
                     /locus_tag="PLG42_07005"
     CDS             complement(4303..4623)
                     /locus_tag="PLG42_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HOU30994.1"
                     /translation="MELSEKQEIESRIAGVLKSVYDPEIPVNIYDLGLIYDINIDDDH
                     VAHITMTLTAPNCPLASQLVEEVKYKVAGTRGVKDCEVNLTFNPPWTRDRLSDAAKLE
                     LGLM"
     gene            complement(4681..5124)
                     /locus_tag="PLG42_07010"
     CDS             complement(4681..5124)
                     /locus_tag="PLG42_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOU30995.1"
                     /translation="MSINDIQDSIIEEFSAFDDWMDRYALLIEKGKELPPLDPAHKKN
                     DYLIEGCQSKVWLYPEFRDGKIYFSADSDALITRGIVSLLIRVLSGRTPEEIKNADLY
                     FIEKTGLRENLSPTRSNGLLAMIKQMKLYAIAFEYNMNKNRSDEA"
     gene            complement(5136..>6246)
                     /locus_tag="PLG42_07015"
     CDS             complement(5136..>6246)
                     /locus_tag="PLG42_07015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOU30996.1"
                     /translation="CVIEAVDEVYRSYNANVHRGVHVLSDMVSEAYENAREKIRDFIN
                     ASKKEEIIFTSGTTASINAVAFSFGERYIKPGDEIIISNLEHHSNIVPWQMMCERKGA
                     RLKVIPVTDRGEIIMEKYVKLLTEKTKLVAITQVSNALGTVVPVKEVTALAHQHGIPV
                     LIDGAQGIQHCQVDIQEIGCDFYTFSGHKIYGPAGTGILYGREKYLEEMPPWHGGGDM
                     IKSVSFEKTVYNDLPFKFEAGTPNYPGFIGLGRAIDYIRELGMEDISNREKRLLDYAT
                     EKLSGIDRVKIIGNAAEKVSVISFVIEGIHQYDAGMILDKLGIAVRTGNHCAQPLIER
                     LGLEGTIRASLCFYNTEEEIDTLAEGIRKVIRMFY"
BASE COUNT         1768 a         1330 c         1113 g         2035 t
ORIGIN      
        1 tatgggttta tcataacttt catcttcgct tcagccattt tgctgccgat ctccatcgca
       61 aaacatgaac ccataaacat cactccggtg tgataagtaa tttttttctc accgggttct
      121 atattgaaag ttgttcggag attcatattt taattattcc gtattacttt ttatccagtc
      181 aataataaca gaaaaaacct catccctgaa aggttcgtta tgaagctcat gatatcctcc
      241 ttcccacaag cggaaggtta ttttgttcga cctggaggca atttccatgc tggctgcagg
      301 tgaagtaatt ttatcttccg ctccgtgaag caataaaaca ggaacgttta tactttctgc
      361 atttttcata atatattcac ctgatgaaac ggcactgaaa aaaaggctaa ccgaaattct
      421 gccatgaaca agcggatcct tttcatactt gtcaacttca tcctgtttgt gtgagagatg
      481 ttctgtaacc agccccgtat gctgtgtgag cgaaggcagg atactcttca ttatacgcgc
      541 aagcgttact tttgcacctg acggtggaaa tgcgagcctt atccatggcg atgtgactat
      601 tgcacctttt atgtgaggat tccttttaat gagataagcc agaactattc ctccacccat
      661 actgtgacca tatataaaaa ccggcctctc cggatagctt tttgcgcttt cttccaaaag
      721 aatatcaagc atttcatcaa tctggtcata gtttttaata tgacctcttt tgccatccga
      781 cctgccatgg cccggaagat caaccccctg aaacattata cctgcagaag tgaactttgt
      841 tgcccagtca ctatatctca ttatatgttc tccgaggcca tgaaccataa taactgaagc
      901 ttttgcttca cctgaagggc tccatttgta ccctcttaac aatagcccat ctgaaagtct
      961 gatatttaaa tccatctgtt atttgtttgt ccataaaaat aataaaaaac acgccgcact
     1021 ctcatcagag gcactttatt atatgccgtc ttataattaa acccagctta atttaatttg
     1081 aattgtaaaa atttttattc gagtaataat ttatactgtt ttttttattc cattgaatat
     1141 atgcaactta aatcaacctg ttatttagaa tcatttatta tttttaagaa tataggatat
     1201 taagatttgt ttatcttaaa ttattttttt atatttgcag tgaacaattg aaaaaaatta
     1261 tcaatatgac gatacatgaa tttaataacc ggcttattga actgaaaggg aaccttcaca
     1321 gatttgcttt aagtctcacc tacaaccgtg aagatgccct tgatcttgtt caggatacat
     1381 atcttaaagc tattgcaaac aaggataaat ttgttgatta tacaaatctg aaagcatggg
     1441 tttttacaat aatgaaaaac acatttatta ataattaccg caggggtcaa aaaggaaaca
     1501 caataataga cagtacaaaa gacctttatt atatcaatct tccccaggac aaaggttcga
     1561 tttcacccga atcgagttat gctgccgaag agattgaaaa agcgatagat tctcttgatg
     1621 atgaatacag gttacctttc agaatgcatg tggaggggtt taagtatgaa gaaatagctg
     1681 aaaagctgaa acttaaaacc ggtactgtaa aaagtagaat cttttttgca aggcagaaac
     1741 tgatgaaaat acttaaggac tacaattaaa agttctgtat attcagatag caggtttctg
     1801 tttctttact ggaaaaatta ttgctcctgc aaatagaata atgaaggttg ctgtaattaa
     1861 ccaggcccag atattataaa ctattgattc agccttcatt atgtaaggtt ctgtaagaaa
     1921 aaattcagat ttaacctgcc ataacgggaa gccagtacta tccttaagca tgctattgct
     1981 actcacctgt tttccaggca ttacaattct tacgctataa ttactgaacc tttgtcccag
     2041 aagatcctca attaccctca ttgccgaatc ggcttcttcc ttaaattcaa ttgcttcctt
     2101 ttcgcctgta agttttttca gaataactcc ctgtgaccag agagaatcaa aatcattcat
     2161 attatccctt accatactgt ataatattgc ttctttctcc ttggtattgc ctttgatacc
     2221 tttcccttct gttaatttat tgaaggtatt aatccattct gaaatcaaac tccgccacat
     2281 ccatatttcc tggtgtgatt ttacactgtc gtccagtact ttgtacctca aactatccgg
     2341 acctattaaa agcctggttt tttctgcttc cggcagataa aaccatttca gttcatcatc
     2401 cggcataaat gaggcaacgg gataaccatg atttattctt ttttcgacaa tttcggaaaa
     2461 actgtaatga gtatcgaacc atctgaattt ctttcgaaat tcaactcgtc ttactgtgct
     2521 cttattgagg ctgctatcgg aagaataata attgttgagt tcttcagcgt tactgaagtt
     2581 tttaacagcc cttcttaccc tgatagtatc tccatttttg ttaatttcca gagaatctct
     2641 tattgaccag gtcgaatcgt atggaacctg aacgtccggg atattgaatt tagcattcgc
     2701 ccctctcatt tcaatccttc tttcaattga cccgtccatc ctaacaatat ctgtgacagt
     2761 tgtttccggt tcattgcatg aatgcaatga cagaaataaa atcagaactg ttttaaatgt
     2821 tttcttcatg aattaatttt tggttataac accttgtatt gttttaaatt gttctaataa
     2881 taaaattaat aaactaatag taaatttaca acgcaaataa tgattagtat gttgcagaaa
     2941 gttatatcat tgagtacgca ttcaaggaac ggattatatg acattacttc gattgttgag
     3001 tctgtggtaa aagaaagtgg tgtaagaagc gggattgcat cggtttatgc aaggggctca
     3061 acctgtgcaa tcatgataca ggagaactgg gacgaatcgg ttcagactga tgtaatcaca
     3121 ctgcttaaca agctgatacc tcatggtgta tgggagcacg acaggcagga caacaacggg
     3181 gatgctcatc ttaaagccgg aattgttgga ccttccgaaa ccattcctgt tgtcgaaggc
     3241 aagctcggac tttcaacatg gcagaacatt tttttctgtg agtttgacgg gcccagaaac
     3301 aggagggaga ttatggtaac cgttatcggt gaaaaataaa atataaaata aagaagtcag
     3361 gctgaatact gaagaataat ttcgacgttt tttttgaatc tctcaaatcc cacacgtgcc
     3421 actggtgttt ttccgaaaag tttcaggaac ttttctctgt cgagtgaaag ccattcctgc
     3481 cttgtcattg aaagtatctc tggcgaagca ttaagctcag gatgattatg gtatgggatg
     3541 tttttattcc atggacatac ctcctgacat atatcacatc cataaactct gcgttcgaac
     3601 agatgtgcta ccccgtagtc tattggattt ctgttttcga ttgtgaggtt tgcaatacac
     3661 tttctggcat caattgtacg gtttgtgttt atagcgcctg ttgggcatct atttatgcat
     3721 tcctcacaac tgccgcatct gtcctctctt actggctcat catattccag atctatatca
     3781 agcattaaaa ttcctatgaa aaaaaaggat ccctttgttt cgtttatcac aagcgaattt
     3841 ttcccctgcc atccaagtcc tgcctcaaca gcccagggct tttcaagcac aggcgatgaa
     3901 tcgcaggagg ttttgccctg aacacccggt acaatagttc ttataaaaga aagaaggctt
     3961 tcgagtttcc cggtaataac gtgatggtaa tctgttccaa gagcatatct cgaaattata
     4021 ggcacgtcca ttccagtctg aatatcagcc gtaaaataac tcatccctgt aacaacgata
     4081 gaccttacac ctggaaaaaa cagagcagga tcaattctcc tggcaatgct tcctgaaaga
     4141 taattcatac tgtcattcat acctgccctg caccattgag tgaagggtcc ttcatattcc
     4201 gaaagtcttc tgtagcgggc tattccgcac aagtcaaatc ccagtgtgta tgctttcttc
     4261 ttaattgctg cggatatatc gcctgtgtta cccattgctg gttcacatta agccaagctc
     4321 gagttttgcg gcgtcactta agcggtccct ggtccaggga ggattaaaag tcagatttac
     4381 ttcacagtcc ttcacacccc ttgtccctgc aaccttatat tttacttcct caaccagttg
     4441 ggaagcaaga ggacaatttg gagcagtaag ggtcattgta atatgtgcca catgatcatc
     4501 gtcaatattt atatcatata tcagcccgag gtcataaata ttaacaggaa tttcagggtc
     4561 gtaaaccgat ttcaatacac ctgcgattct cgattctatt tcttgctttt ctgacagttc
     4621 catggcaacc tgatttttta atttttaaac taacatttta gtaattaaag ctcgcgttta
     4681 ttaagcttca tcagaacggt ttttattcat gttatattca aaagcaattg cataaagttt
     4741 catttgttta atcattgcca gcagtccgtt cgagcgcgtg ggagagaggt tttccctgag
     4801 tccggtcttt tcaataaaat aaaggtctgc gttttttatt tcctcaggag tcctgcccga
     4861 gagaacacgg ataagcaggg aaactattcc tcttgttatc agtgcatcac tgtcggcact
     4921 gaagtatatt ttcccatccc tgaactcagg gtagagccac actttcgatt ggcatccttc
     4981 aataaggtaa tcattctttt tatgtgcagg gtcgagaggt ggtagttcct ttcccttttc
     5041 tatcagcaat gcatatctat ccatccagtc gtcaaatgcg ctgaactctt caattatact
     5101 atcctgaata tcgttaatgc tcataaacat cttcattaat aaaacatcct gattacttta
     5161 cggatgcctt ctgcaagggt atctatttct tcttcggtgt tgtaaaaaca caaagatgct
     5221 cttattgtcc cttcaagtcc tagtctttca atcagcggct gggcgcagtg attacctgtc
     5281 cttactgcta ttcccagctt gtcaagaatc attccggcat catactggtg aataccttcg
     5341 ataacaaagg aaattactga aactttctca gcggcatttc cgattatttt aaccctgtcg
     5401 attccagaaa gtttctcggt agcataatcc agtagccgtt tctcgcggtt gctgatatct
     5461 tccattccca gttccctgat gtaatcaatt gctcttccca gtccgataaa ccccggatag
     5521 ttgggtgttc ctgcctcaaa cttgaaaggc aggtcattgt atactgtctt ttcaaagctc
     5581 acacttttaa tcatatcacc acctccatgc cagggtggca tctcttcaag atatttttcc
     5641 ctgccgtaaa gaattcctgt tcctgcaggg ccatatattt tatgacctga aaacgtataa
     5701 aaatcgcagc cgatctcctg tatatcaacc tgacaatgct gaatgccctg tgcaccgtca
     5761 atcagtactg gtatgccatg ttggtgagct aacgcagtaa cttcttttac tggcacaact
     5821 gtacccagag cattcgacac ctgtgtaatt gcaacaagtt tagttttttc agttagcagt
     5881 ttaacatatt tctccattat tatttcaccc ctgtcggtaa ctggtattac cttaagccgt
     5941 gctcctttcc tttcgcacat catttgccag gggacaatgt tggaatggtg ttccagattg
     6001 cttattatga tttcatcgcc gggtttgata tatctttcgc cgaatgaaaa agcaacagcg
     6061 ttgattgagg ctgtagttcc tgaggtgaaa attatttctt cctttttgga tgcgttgatg
     6121 aaatctctga ttttttctct tgcgttctca taagcttccg agaccatatc gctgagtaca
     6181 tgaactcctc tatggacatt tgcattgtaa cttctgtaga cctcatcaac agcctcgatg
     6241 acgcat
//
LOCUS       DAOSNB010000152         6224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_12679_length_6224_cov_4.911493, whole genome shotgun sequence.
ACCESSION   DAOSNB010000152 DAOSNB010000000
VERSION     DAOSNB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12679_length_6224_cov_4.911493"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLG42_07020"
     CDS             <1..518
                     /locus_tag="PLG42_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU30997.1"
                     /translation="RFENHGIGRKKDVSRKDSEVIKALHQTIKKVGNDIENLRFNTAI
                     SAMMIFINLATKKGKITRRTAETFIKILSPFAPHLAEELWNLAGHKKTIAYEPWPEYN
                     EEYLKEDTFEYPVSFNGKLRFKLELPLSMDKEEIVDRVVSDPRSAKWLSGKKPENVIV
                     VPGKIINVVVR"
     gene            534..1814
                     /locus_tag="PLG42_07025"
     CDS             534..1814
                     /locus_tag="PLG42_07025"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOU30998.1"
                     /translation="MSQKILKLFRIKKVTAGVLFLLLLVLISDGSCRQETEPALQLRP
                     EIAATDSTPYQNNEPVYIYGIPANSFNIINGIIKPNQRLSDILLPLGISGYELDQLTR
                     NSKEVFDVRKIRARHNYIILTDNDSAAKVRYFIYEHDPDLSYIFSFNDSLKITEWKTE
                     SLSVIRFSSFTIETSLWEAMKRAGVNPELAVALSDIFAWTVDFFGLQRGDKFKVIYEE
                     KQIRDDVTLPGRIYAAMFISGNREYMAIPFVQDSIESYYDYNGESLKKAFLRAPLKFS
                     RITSRYSSARLHPILRIVRPHHGIDYAAPVGTPVYSVGDGRVTETGLDNESGKYVRIK
                     HNSVYSTAYLHLSSFGKGITPGAFVKQGDIIGYVGTSGLSTGPHLDFRFYKNGYPVDP
                     LKVEAPPVEPVREENIEKFIKIRHVIALLLSQIE"
     gene            complement(1848..2420)
                     /gene="purN"
                     /locus_tag="PLG42_07030"
     CDS             complement(1848..2420)
                     /gene="purN"
                     /locus_tag="PLG42_07030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOU30999.1"
                     /translation="MKHIAIFASGSGSNAENIIRYFNDKKNAKVALVLSNKPDAYVIK
                     RAEKLNVPVKVFNREDFYNSNNVLDTLISYNIDFIVLAGFLWLVPENILRHYKRRIVN
                     IHPALLPLYGGKGMYGDRVHRAVIESGDSESGITIHYVDEKYDNGDIIFQVRCRVEPG
                     ETPESLAAKIHELEYKYYPQIIEKLILEAK"
     gene            2606..2857
                     /locus_tag="PLG42_07035"
     CDS             2606..2857
                     /locus_tag="PLG42_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOU31000.1"
                     /translation="MSEFPKSDIAKRVKEIIVEKLGVDESEVTPEASFTNDLGADSLD
                     TVELIMEFEKEFNIGIPDDQAETINTVGEAIKYIEENAK"
     gene            2877..4130
                     /gene="fabF"
                     /locus_tag="PLG42_07040"
     CDS             2877..4130
                     /gene="fabF"
                     /locus_tag="PLG42_07040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOU31001.1"
                     /translation="MFMKRVVVTGLGALTPIGKTLHEYWEGLKNGVSGSDWITRFNTE
                     KFKTRFACEIKNYDSSQYFERKEANKLDIYSQYALIAAEEAIKDSGLDLERINKDRVG
                     VIWTSGIGGLETFYESIKSHVLGDGTPRFSPYFIPKMISNIAAGNISIKYGFRGPSFS
                     TVSACASSTNAIIDAVNYIRLGKADVIVTGGSEAAINEAGVGGFNSMQALSTNNEEYK
                     TASRPFDKTRDGFVMGEGAGALIVEDYEFAKARGARIYAEIAGTGLSADAYHLTAPHP
                     EGLGAILVMKNALEDAGLRPEDIDYINAHGTATPLGDIAETKAILSVFKEHAYRMNIS
                     STKSMTGHLLGAAGAIEAIASILAVVHDIVPPTINFKTPDPEIDPNLNITPNKAQQKR
                     IYAALSNTFGFGGQNASIIVKKAEE"
     gene            4130..4873
                     /gene="rnc"
                     /locus_tag="PLG42_07045"
     CDS             4130..4873
                     /gene="rnc"
                     /locus_tag="PLG42_07045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOU31002.1"
                     /translation="MPLFKKRKNGTLILDRKEFASHIRKLLGVRSGNLPLYEMALIHR
                     SATVTLPDGRRVNNERLEFLGDSILNSILSDWIFENFPDATEGNMTKIRSKIVNREFI
                     NEIAIKLGINKILVSNISPSNTTRNLYGNAFEALIGAAFLDKGYQGTKKFLINRVLKK
                     YLEIEKILNTDSDYKSLILEWAQKTKQNLQFSTTENYDNFAKKTLFTSELLINDVEVG
                     RGTGTSKKEAEQEAACMAWIKLNKITEKH"
     gene            4917..5351
                     /locus_tag="PLG42_07050"
     CDS             4917..5351
                     /locus_tag="PLG42_07050"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOU31003.1"
                     /translation="MDDRKKPVRLKLDIQDTDENLVLIGIVSSEPDYKLSLALNKKLN
                     ISLKLSNPIELTDRKGNISSFSRFADTSEMPEISYTLVSNRSGNDVLFKKMKKLDYIF
                     SISHSYPILHAGNIIRNIREIEGVTAAFELDSKEFDYRLLQN"
     gene            5363..5851
                     /locus_tag="PLG42_07055"
     CDS             5363..5851
                     /locus_tag="PLG42_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31004.1"
                     /translation="MEKVFYDLTEHEFTKGRKILLWIFCSSFFLIGVAIIFMNIIEHK
                     LAIHISYSIAPFGISIFTGIIAYLSTAKRKNHFFLIDDEKIEYRYGIIFPELKTHKWN
                     EIKEIHIPHRGKKILLVYNENKKNIINLTWLERKKSFMIKRHIYAFARNKNIKIVTVS
                     AL"
BASE COUNT         2148 a         1172 c         1143 g         1761 t
ORIGIN      
        1 gccggtttga aaatcacggc attggccgaa aaaaagacgt cagcagaaaa gattcagaag
       61 tgattaaagc actccaccag actataaaaa aagtcggaaa cgatattgaa aatctacgct
      121 ttaacactgc tatctcagca atgatgattt tcataaatct ggcaacaaaa aaaggcaaaa
      181 ttaccaggag aacagcagaa acatttatta aaatattatc accctttgct cctcaccttg
      241 ctgaagaatt atggaatctt gcaggacaca aaaaaaccat tgcatacgag ccctggcctg
      301 aatacaacga agaatacctg aaagaagaca catttgaata cccggtttca ttcaatggca
      361 agctcaggtt taaactggaa ctgcctctct cgatggacaa agaagaaata gttgaccggg
      421 ttgtttctga tccaagatct gcaaagtggc tttccggtaa gaagcctgaa aatgtcattg
      481 ttgttcctgg taaaataata aatgtggttg tcaggtaaac tcaccggcct ttgatgagtc
      541 agaaaatact aaaattattc aggattaaaa aggtaacagc aggtgtacta tttcttctgc
      601 tgctggtctt aatatctgat ggttcctgca ggcaggaaac ggaacctgcg ctacaactac
      661 gacctgaaat cgctgctaca gattcaacgc cttatcagaa caatgaaccg gtttatatct
      721 acggaattcc agccaattca ttcaatataa taaatggtat aattaagcca aaccagcggc
      781 tttccgacat tctgctcccc cttggtattt caggttatga gcttgatcag ctgaccagaa
      841 attcgaagga agtatttgac gtaaggaaaa tacgtgcacg gcataattat ataatactta
      901 ccgataacga cagcgccgct aaagtgaggt attttatata cgaacacgat cctgatcttt
      961 catatatatt cagttttaac gactcactaa aaataactga atggaaaact gaatcattat
     1021 ctgtaattcg gttcagttct ttcaccattg aaacttcact ttgggaagcg atgaaaagag
     1081 ccggggtaaa tcctgaactt gctgtggcac tctccgacat tttcgcatgg acagttgatt
     1141 tttttggact gcagcggggt gataaattca aggtaatcta tgaagaaaaa cagatccgtg
     1201 atgatgttac ccttcccgga agaatatatg ctgcaatgtt tatctcaggt aaccgggaat
     1261 atatggcgat tccatttgta caggacagta tcgaaagtta ttatgattat aacggtgaaa
     1321 gtcttaagaa ggcttttctg cgagctccgc ttaaattcag caggataact tcgcgatatt
     1381 cgtcagccag gcttcatccc attttaagga tagtgcggcc gcaccatgga atagattatg
     1441 ctgccccagt aggaacacct gtttattctg tgggagatgg aagggtaact gagacaggac
     1501 ttgacaatga atcgggtaaa tatgtcagaa taaaacataa tagtgtctat tcaacagctt
     1561 acctgcatct tagctcgttc ggcaaaggca ttacacctgg agcttttgta aaacagggag
     1621 atataatagg ttatgtagga acgagcggat tatcaacggg gccacatctc gatttcaggt
     1681 tttacaaaaa cggctatcct gtcgacccgc ttaaagttga agcaccaccg gttgaaccag
     1741 taagagaaga aaatattgaa aaattcatca aaataagaca cgtaatagcc cttctgttga
     1801 gtcagattga ataaatcgca ataatttatc ctattttatt tattgattta tttagcctca
     1861 agtataagct tctcaataat ttgtgggtaa tatttgtatt caagctcatg aatcttggct
     1921 gcaagcgatt caggtgtctc gccaggttca accctacacc ggacctgaaa tataatatca
     1981 ccattatcat acttttcatc cacatagtgg attgttatac ctgattcact atcacctgat
     2041 tcaatcacag ccctgtgaac ccggtcacca tacatgccct ttcccccgta caaaggcagt
     2101 agtgcaggat gaatatttac aattctccgt ttgtaatgcc tgagaatatt ttctggcacc
     2161 aaccataaaa atcctgcaag tacaataaaa tcaatattgt atgaaataag tgtatcaaga
     2221 acattattac tattataaaa atcttcacgg ttaaagactt ttaccggtac attaagcttt
     2281 tcagctcgtt taattacata tgcatcaggt ttatttgaca gaaccagagc aacttttgca
     2341 ttttttttgt cattaaagta ccttataata ttctctgcat ttgaacccga acctgaagcg
     2401 aatattgcta tatgtttcat ttactgatta ttataaaaat tgtgaaattt gacaataaaa
     2461 taaacctcag ttatttatgt atttgaatat caattttata acatattttt tgtaaaattt
     2521 ttcgcttttc atattgaaaa ttattttaca aatatatttc tttgcactat ctttaaaaat
     2581 caggtattaa ttaaaattta agactatgtc agaatttccg aaatcagaca ttgcaaagag
     2641 agtaaaagag atcatcgttg agaaacttgg agtcgatgaa tcagaagtaa ctcctgaagc
     2701 aagcttcaca aatgatcttg gggccgattc gctggataca gttgaactta ttatggaatt
     2761 tgagaaagag tttaacattg ggattcctga cgatcaggct gaaactatca acactgtagg
     2821 tgaagctata aagtacattg aagagaacgc aaagtaggtt aaagttctcc aatcatttgt
     2881 ttatgaaaag agtcgtagtt acaggtctgg gagcactgac tccaataggc aagactttac
     2941 atgaatactg ggaaggcttg aaaaatggag tcagtggttc tgactggatt accaggttca
     3001 ataccgaaaa gtttaaaaca cggtttgcct gtgaaattaa gaactacgat tccagtcaat
     3061 attttgaaag aaaagaagca aacaagcttg acatttattc tcagtatgcc cttattgcag
     3121 cagaagaagc tattaaggat tcagggcttg accttgaaag aataaacaaa gacagagtag
     3181 gggtcatttg gacatcgggc attggaggac tggagacatt ctatgagtca atcaaaagcc
     3241 atgttcttgg tgacggtact cccaggttca gtccgtattt tattcccaag atgataagca
     3301 acattgctgc aggcaacatt tcaataaaat acggatttcg aggaccaagt ttttcaaccg
     3361 tttcagcatg tgcatcctca acaaatgcaa ttatcgatgc tgttaattat attaggttgg
     3421 ggaaagcaga tgtaattgta accggtggct ctgaggcggc aataaatgag gcaggtgttg
     3481 gaggattcaa ctcaatgcag gctctctcaa caaacaatga ggaatacaaa actgcatcaa
     3541 ggccttttga taaaacaaga gatggttttg ttatgggtga aggtgctggt gcacttattg
     3601 tggaagacta tgaatttgcc aaagcaagag gggcaagaat atatgctgaa attgcaggca
     3661 caggtttatc agccgatgca tatcacctca ccgctccgca tcctgaaggt ctgggtgcaa
     3721 tactggttat gaaaaacgca cttgaagatg caggtctcag acctgaagac attgattaca
     3781 taaacgctca cggaacggca acccctcttg gcgacatagc agaaacaaaa gccatactat
     3841 ctgtttttaa agaacatgcc tacaggatga acatcagctc aacaaaatcc atgacagggc
     3901 atctactggg agcagccggc gctattgaag ccatagcctc aattcttgct gttgttcacg
     3961 acatagtgcc tccaactatt aacttcaaaa cacctgaccc tgaaatagat ccaaatctta
     4021 acattacacc gaataaagct cagcagaaaa gaatttatgc tgcattgagc aacactttcg
     4081 gcttcggagg ccagaatgct tccataattg ttaaaaaagc agaggaatag tgcctctctt
     4141 taaaaaaaga aaaaacggaa ccttaattct tgaccgaaag gaattcgcat cgcacatcag
     4201 aaaacttctt ggtgtacgct cgggtaattt acccctctat gaaatggctc tgatccaccg
     4261 gtcagctaca gttacgcttc ccgacggaag aagagtcaac aatgaacgtc ttgaatttct
     4321 gggcgattcg atcctgaaca gtattttatc cgactggatc ttcgaaaact ttcccgacgc
     4381 taccgaaggt aatatgacaa aaatcagatc caaaatcgtt aacagggaat ttataaacga
     4441 aatagccata aaactgggaa tcaacaaaat actggtctct aatatttcac catccaacac
     4501 caccagaaat ctttatggca atgccttcga agcacttatc ggtgccgctt ttctcgataa
     4561 aggttaccag ggtactaaaa aattcttaat taatagagta ttaaagaaat atctcgaaat
     4621 agaaaaaata ttaaacaccg acagcgatta caagagcctc atactggaat gggcacagaa
     4681 aacaaaacaa aatcttcaat ttagtactac ggagaattat gataattttg caaaaaaaac
     4741 actttttact tcggaactgt tgataaacga cgttgaagtc ggcagaggaa caggaacatc
     4801 caaaaaagaa gccgagcagg aagccgcctg catggcatgg ataaaactca ataaaatcac
     4861 tgaaaaacat taaattaatt tttttattat atttagtgtc actttatttg agttaaatgg
     4921 acgacagaaa gaaacctgtc cggctgaaac ttgatattca agatactgac gaaaaccttg
     4981 tgctgatagg cattgtttca tctgagcctg attataaact atcacttgca ctaaacaaaa
     5041 aactgaatat atcacttaaa ctaagcaatc caatcgaatt aactgaccgc aaaggaaata
     5101 tatcttcatt cagcagattc gctgatactt cagaaatgcc agaaatctcc tatacactgg
     5161 tatcaaacag atcaggaaat gatgtcctgt tcaaaaaaat gaaaaagtta gactatatct
     5221 tctcaattag ccattcatac ccgatccttc atgctggtaa tataatccgc aacatcaggg
     5281 aaatagaagg agttacagct gcctttgaat tagacagcaa agaatttgat tacaggttat
     5341 tacaaaatta aaaaatctga taatggaaaa agttttttac gacctcactg aacatgaatt
     5401 caccaaaggc agaaaaatat tgctttggat attttgttct tccttttttc ttataggagt
     5461 tgctattatc ttcatgaata ttattgaaca taaattggca attcacatct catactccat
     5521 tgctcctttc gggataagta ttttcacagg aataattgca tacctgtcga cagcaaaacg
     5581 caagaatcac ttttttctta ttgatgacga gaaaattgaa tatcgctatg gaataatatt
     5641 tccagaattg aaaacacata aatggaatga aattaaggaa atacatatcc cacaccgggg
     5701 caaaaaaata ttacttgttt acaatgaaaa caaaaaaaat ataattaacc ttacctggct
     5761 ggaaaggaaa aaatcattta tgattaaaag acatatatat gcctttgcca gaaacaaaaa
     5821 cataaaaatt gtaacagttt ctgcacttta gaaatttatt ttcgctgaaa tgtaaaaaag
     5881 aagagccgcc agaatagcgg ctcacttttt tgataaccct aaaactaacc taacctatga
     5941 aaaaaacatc tgtttaacag ctctcaaaaa ctgtgccaaa tgcattgcaa agataaaaat
     6001 aaaaattttt tcaaacaaat gtttttatcg aacatcttga tttttaactt ttttttaata
     6061 tttatttaac atgatagatt tttaatccat cccagccata ttccacgggg aaaaatcaaa
     6121 ttcagtgtta aagaaagtta aagattgtta ataaactcgc aagttaaact tacttttgta
     6181 acggtgaaac gaaatcatat actgttgcta gctcttttct tcct
//
LOCUS       DAOSNB010000153         6089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13067_length_6089_cov_5.888797, whole genome shotgun sequence.
ACCESSION   DAOSNB010000153 DAOSNB010000000
VERSION     DAOSNB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13067_length_6089_cov_5.888797"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1835)
                     /locus_tag="PLG42_07060"
     CDS             complement(177..1835)
                     /locus_tag="PLG42_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31005.1"
                     /translation="MLTSYDHFKVEIKNVKYFAEDGRVCPRTAIISFYDSTGKEISNE
                     LFGVIEKQEIYRMIEEGKDLILDNLYINDFSLSAYRRIHNLDKKSHVRINNLSARNSF
                     FEATTATDFSFALFNEGDISFEGSHFARGQVLFTGCDFGKGNVNFSNTFFRCGDIDFS
                     GSLFDDGDFLFKNSVVKDGKKDFQDIRFGNGEINFSNTEFNSGELLFINTHFGNGHFI
                     FKVARIASGKVDFHYSSFGDCDVSFERAEFGNSKVDFRTVDFGSGKINFNRSIFGNGD
                     VTFEGASCRSGKIQFKRVEMGSGHKNFSLMEMPNAEINFEKTNFGDGILTFNGSQFKL
                     LCLRSCNFDHYVDLRLAKAEILDISDTIVRDIIDLEPYDFDVNIDVINLTGMRLIGKI
                     YIDWHKNRCYDKIMAQEDTTVRQKAEQFRTLKENFSNTGKYSFEDKAYVAFRRLESIA
                     DLKESVSKNKWSALWSYPAYGFKWLVFDKIGLYATSPGRVLVSVVLIWFLFGFIYYII
                     QLSGLGMTMSSVNNPDKLTPFMQSFYHSAITFLQSVMAMFFHRG"
     gene            complement(1886..4765)
                     /locus_tag="PLG42_07065"
     CDS             complement(1886..4765)
                     /locus_tag="PLG42_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOU31006.1"
                     /translation="MEYFLEHLAKHIYKNYSETLEKQCLVFPNRRAGLYFMKYLSDII
                     KKPVWSPAIKTINELFSYLSQLEIAENEILIFELYKTYKEISERLKKKPEPFDEFYFW
                     GEMLLNDFDDVDKYMVDAGKLFSNLSDLKRIDTELGGLTEEQIEIIRQFWVNFNTGKP
                     TGHKEMFLELWEILPELYVKFCNNLRNKGLAYEGMIFREVAEKCISGENLSLPWERFY
                     FIGFNALNRCEKELFKYLKNNGKAKFYWDYDESYIDKSSSHTAGYFIRENLGLFGNDM
                     PDGWNYKTLLSASGTKRKIISTSTDIAQVKLVPELLKELGNLNANEAHHTAIILADEN
                     LLLPLLNSIPDSDVDVNITMGYPLKFSPVYSFLRLLLLLQRNSRKENEEILFEYSDVS
                     GLFQHSFLSDEAKYQSGEILKSLIKEGKRWIPSSKLCRTESLKMIFRKVESPGEIASY
                     LKTILMSFYLAGEDEPGLSIRSKLHNEFIFRSLQVINRLENLTRTAEIEMKTETWLLL
                     LDRILRNITVPFSGEPLSGIQIMGLLETRTLDFKNIIMLSVNEGVLPRASAGSSYIPY
                     TLREAFGLPVIRHQDSIYAYYFYRLLHRAENVTFVYNSNAEGLKTGEMSRFLMQLIYL
                     YENHPDILNTGYKIGVKLLPEGEIERSEKHIKILEKIYFENKEPLSPNAINTWISCRM
                     KFYYRYVCGIKEPERIISADDAATFGEILHQTMKELYSGYLNKTLEKKDIEKLAEKSD
                     IDELVKKLLRAKFHSNTDAVLSGTELIIASILSYHVRLILNMDINVTPFKICGLEKKM
                     SAPFEINYDGNKKEIFIGGIIDRIDEKSGSFRIVDYKTGKVKKSEIESFDSLFEENEE
                     ERGQEWLQVLIYCEIFLNKKDNTKVFPLIYSLRKLTEEEYTGMLKIKNNNNEIIVDDY
                     SKFRNGFKSKLEKTVSSIFDKDEPFRMTENTKICEKCLYRELCNR"
     gene            complement(4749..5765)
                     /locus_tag="PLG42_07070"
     CDS             complement(4749..5765)
                     /locus_tag="PLG42_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246595.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOU31007.1"
                     /translation="MRELIHTEKKNSLFPWGTSRRYNSYGDFLNIKFGGRVQKISIDG
                     GFTCPNRDGTKGTGGCVFCDNRAFNPPYCDPGKSVTQQLKEGIAFFSKRYRKTRGYLV
                     YFQAYSGTYSDISTLEKIYYEALLFPEVKGIIIGTRPDCIDSEKIELLQNIGEKYFVA
                     VEYGIESCRDDTLRRINRGHTFSDSVKAVKMTAEANIHTTAHLIFGLPGETPDEMLEE
                     AEIISELPLNSVKFHQLQIIKGTVAERQYMENPGEFKIFTLDEYVEFIIAFLERLNPG
                     IAIERFTAEVPGEMLAVRPWNNTPSEYITRMIEKRMEAQNTWQGRLYKHKKTERQNNY
                     GIFS"
     gene            complement(5753..>6089)
                     /locus_tag="PLG42_07075"
     CDS             complement(5753..>6089)
                     /locus_tag="PLG42_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOU31008.1"
                     /translation="QEGRIDEGEKEEIEKRIKSAVSSDEIREWFRPGLKIVTEADILT
                     TKGTIRRPDRVIISDDRAIVVDFKFGRERSEYKEQVALYRNLLLEMGYKQVDAYLWYV
                     DQGKVVCVN"
BASE COUNT         1782 a         1262 c         1037 g         2008 t
ORIGIN      
        1 gggaggaaaa cacggcaata agggtgatga agctgccgta actgcaataa agatggcagc
       61 gctgaaaaga gatatgactg aatagtttta cctcagcact tttcttacga aagccaccgt
      121 gaagtaggac atcataaaaa caccgataaa accttcaatt cctgaaaaaa ttctgctcag
      181 cccctgtgga aaaacatcgc cataaccgat tgtaaaaaag ttattgcaga atgataaaat
      241 gattgcatga aaggtgtgag tttatcggga ttattgacag aagacattgt cattccaagt
      301 cctgaaagtt gaatgatata gtagatgaaa ccgaacagaa accagataag taccacgctt
      361 acgagtactc tgccgggaga tgtggcataa agtccaattt tatcaaatac aagccattta
      421 aaaccataag ccgggtaact ccagagggcc gaccatttgt ttttcgatac tgactctttc
      481 agatcagcaa tggattcaag ccggcgaaaa gcaacataag ctttatcttc gaatgaatat
      541 ttacccgtat tactgaaatt ttccttaagt gttctgaact gttcggcttt ctgccggact
      601 gtagtatctt cctgtgccat tattttgtca tagcatctgt ttttgtgcca gtcgatgtat
      661 attttaccga tgagcctcat gccggtaaga ttaataacat caatgtttac atcaaagtcg
      721 tatggttcaa ggtcaataat atctcttact atagtgtcgg aaatgtcaag aatttcagct
      781 tttgcaagtc gaaggtcaac gtaatggtca aagttacatg aacggagaca cagaagcttg
      841 aattgtgaac cgttgaaagt gaggataccg tccccgaaat ttgttttctc aaaattgatt
      901 tcagcatttg gcatttccat caggctgaaa ttcttatgcc ctgaccccat ttcaaccctc
      961 ttgaattgta ttttacctga acggcatgat gctccttcaa aagttacatc gccgtttccg
     1021 aatattgaac ggttaaagtt aattttacct gagccgaaat cgacagtcct gaaatccact
     1081 ttgctgttcc cgaattcagc tctttcgaac gacacatcac aatctccaaa tgatgaatag
     1141 tgaaaatcaa ccttgccact ggctattctt gccaccttaa agatgaaatg cccgtttccg
     1201 aaatgtgtat taataaaaag cagctcacca ctgttaaatt cagtatttga aaagtttatt
     1261 tcaccgtttc cgaatcgtat gtcctgaaaa tctttctttc catcttttac gactgagttt
     1321 ttaaaaagaa aatcaccgtc atcaaacaga gagcctgaaa aatcaatatc accacatctg
     1381 aaaaatgtat tcgaaaagtt tacatttccc ttaccgaaat cgcagccagt aaataatacc
     1441 tgtcctcttg caaaatgtga accttcgaaa ctaatatctc cttcgttgaa taaagcaaaa
     1501 ctgaagtctg ttgccgtagt tgcctcgaaa aatgagttcc tggccgacag gttgtttatc
     1561 ctgacatgtg attttttatc aagattatgt atgcgcctgt aagctgagag ggaaaagtcg
     1621 tttatataaa gattgtcaag tataagatct ttaccttctt caatcatcct atagatttcc
     1681 tgcttctcaa taacaccaaa taactcattg cttatttctt tccctgttga atcataaaaa
     1741 cttattattg cagtacgtgg acaaactctt ccgtcttcgg caaaatactt gacattcttt
     1801 atttcaacct taaaatgatc gtaacttgtt agcatctgtt caggaattaa aaatcaatca
     1861 aaattagtaa aatatcaaaa tagacttacc ggttacaaag ctccctatat aaacattttt
     1921 cgcatatttt cgtgttctca gtcatcctga atggttcatc tttatcaaaa attgaggaaa
     1981 cagttttttc cagttttgat ttaaatccat ttctaaattt tgaatagtca tcaacaatta
     2041 tttcgttatt gttatttttt attttgagca ttccggtata ctcttcttcg gtcaactttc
     2101 tcagagaata tattaaagga aacactttcg tgttatcttt cttatttaaa aatatctcac
     2161 aatatatcag aacctgaagc cattcctgtc cgcgttcttc ttcattctct tcaaagagag
     2221 aatcaaatga ctcaatctct gattttttaa cttttcctgt cttataatca acaatacgga
     2281 acgaacctga tttttcatca attctgtcga ttattccacc gatgaagatt tccttcttat
     2341 tgccatcgta gtttatttca aagggggcag acattttctt ttcaagaccg catattttga
     2401 acggtgtaac atttatgtcc atgttaagaa taagtcttac atgataactc aggatgctgg
     2461 caataattag ttcggtgcct gaaagaacgg catcagtatt tgaatggaat ttcgcccgta
     2521 aaagcttttt aactaattca tcaatatcac ttttttctgc cagtttttca atatcttttt
     2581 tttcaagagt tttatttaaa taacctgaat ataattcttt cattgtttga tgaagtattt
     2641 ccccaaatgt tgctgcatca tcggctgata ttattctttc cggttctttt ataccgcaaa
     2701 catatctgta ataaaatttc atcctgcagg aaatccatgt gttgatagca tttggagaaa
     2761 gaggttcttt gttttcaaaa tatattttct caagaatctt tatgtgcttt tcgctacgtt
     2821 caatttctcc ctctggcagt agtttcacgc caattttata ccctgtattt aaaatatcag
     2881 gatgattctc ataaaggtag ataagctgca ttaaaaacct gctcatctct cctgtcttaa
     2941 gtccttcagc attagaatta tatacaaaag tcacattctc agccctgtga agtaaccggt
     3001 agaaatagta ggcatagatt gaatcctgat gtcttataac tggcagcccg aatgcttcgc
     3061 gcagagtata tggtatgtag gatgagccag cagaggcccg tggaagaact ccctcgttta
     3121 cagacaacat tattatattc ttgaaatcga gtgttctggt ttcgagcaat cccataatct
     3181 gtatcccgga aaggggctca ccagagaatg gaacagttat attccggaga atacggtcga
     3241 gaagcagtaa ccatgtttca gtcttcattt caatctctgc cgtccttgtc aagttttcaa
     3301 gtctgttaat tacctgcaga gatctgaaaa taaactcatt atgaagctta cttctgatac
     3361 ttaaccctgg ttcatcctcc cctgccagat aaaatgacat aagaattgtt ttcagatatg
     3421 aagctatttc tcccggagat tctacttttc taaagatcat ttttagcgat tcggtccgac
     3481 acaattttga agaaggtatc caccgttttc cttctttaat taaagatttg agaatttctc
     3541 cactctggta ttttgcttca tcagacagaa aactatgctg aaacaaacct gaaacgtctg
     3601 aatattcaaa caggatctct tcgttttctt ttctgctgtt tctctgaagc aggagaagca
     3661 atctgaggaa gctgtataca ggactgaatt taagaggata gcccatggta atattgacat
     3721 ccacatcgga gtcgggaata gaattaagga gtggcagtag cagattttca tcagcaagga
     3781 taattgcagt atgatgtgct tcgttggcat ttaaattccc aagctcctta agcaattcag
     3841 gtacaagttt aacctgcgca atatcagttg aggtacttat aatctttctt ttagtcccag
     3901 atgcagatag cagagtttta taattccagc cgtcaggcat atcatttccg aaaagcccca
     3961 ggttttctct tatgaaatat cctgctgtat gactcgatga cttatctatg tatgattcgt
     4021 cataatccca gtaaaacttt gcctttccat tatttttgag atatttaaaa agttcttttt
     4081 cgcatctgtt cagtgcattg aaaccgataa aatagaatct ttcccagggc agagaaaggt
     4141 tttcacctga aatgcatttt tcagctactt ccctgaaaat cattccttca taagcaagac
     4201 ccttattcct caaattattg cagaatttga catatagttc aggaagaatt tcccatagtt
     4261 ccaggaacat ttccttatgc ccggtaggtt ttccagtatt aaaattaacc cagaattgcc
     4321 ggatgatctc tatctgttcc tcagtaagtc cgccaagctc agtatctatt ctctttaaat
     4381 ctgacaggtt tgaaaacagt tttccggcat cgaccatgta tttatcaaca tcgtcaaagt
     4441 cattaaggag catctcgccc cagaaataga attcatcaaa gggttcaggt ttcttcttta
     4501 atctttctga aatctcttta tatgttttat aaagttcgaa tatcagtatc tcattttcag
     4561 caatttccag ctgcgacaaa taactaaata gttcgttaat agtcttgata gcaggcgacc
     4621 agactggttt tttgattatg tctgaaagat atttcataaa ataaaggcct gctcttctgt
     4681 ttggaaaaac cagacattgc ttctccagtg tttcactgta attcttataa atatgttttg
     4741 caagatgctc aagaaaatat tccataatta ttttgtcttt cagttttttt atgtttatac
     4801 aatcgaccct gccatgtatt ttgggcctcc atccgtttct caatcatacg ggtaatatat
     4861 tccgacgggg tattattcca tggtctgaca gcaagcattt ctccaggcac ctcagcagta
     4921 aaccgttcta tggctatccc gggattaagg cgctcaagga atgcaataat aaactcaaca
     4981 tattcatcaa gagtaaaaat cttaaattct ccgggatttt ccatatactg gcgttctgca
     5041 acagtaccct taattatctg aagctgatga aacttaaccg agttgagcgg taattctgaa
     5101 atgatttcag cttcttcaag catttcgtca ggtgtttctc ctggcaatcc gaaaataaga
     5161 tgtgccgttg tatgtatatt agcctctgcc gtcattttta cagcttttac ggaatcgctg
     5221 aatgtgtgtc ccctgttaat cctccgcaat gtatcatccc ggcaggattc aattccatat
     5281 tccacagcga caaaatattt ttctcctatg ttctgcagaa gttcaatttt ttcgctgtcg
     5341 atacaatcgg ggcgtgttcc gatgataatt ccttttactt caggaaataa aagggcttca
     5401 tagtagattt tttcaagtgt gctgatatca gaatatgtac ctgaataggc ctggaaataa
     5461 acaagatagc ctctggtttt gcgatatctt ttactaaaaa atgcaatacc ttcctttagc
     5521 tgttgagtta cactcttacc gggatcgcaa tatggagggt tgaaggccct gttatcacag
     5581 aaaacacatc cgccggtccc ttttgtaccg tcgcggttcg ggcatgtaaa tcctccatca
     5641 atgcttattt tctgaactct tcctccgaat tttatattaa ggaaatctcc ataagaatta
     5701 tatctgcggg acgtacccca gggaaacaga gaatttttct tttcagtgtg aattaattca
     5761 cgcatactac tttaccctgg tcgacatacc acagataagc atcaacctgt ttatatccca
     5821 tctctaaaag aagattacgg tacagagcaa cctgctcttt gtactcactc ctttccctgc
     5881 cgaatttaaa atccactacg attgccctgt catctgaaat aataaccctg tcgggtctgc
     5941 gtatcgtgcc ttttgtggtt agaatatcag cctccgttac aattttgagt ccgggccgga
     6001 accattccct gatttcatca gaggatactg cacttttaat tcgtttctct atctcctcct
     6061 tctctccttc atctatcctt ccttcctgt
//
LOCUS       DAOSNB010000154         6080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13098_length_6080_cov_5.005477, whole genome shotgun sequence.
ACCESSION   DAOSNB010000154 DAOSNB010000000
VERSION     DAOSNB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_6080_cov_5.005477"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLG42_07080"
     CDS             <1..672
                     /locus_tag="PLG42_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31009.1"
                     /translation="KGRCLLPGAYYAITTDRKTVVERYFSSDEKNISNVSRFPSMPDD
                     EGHLILFNKSLEVVDEAYYSEKQHFALLTSVEGVSLEKIMPQAESSSRSNWYSASESS
                     GWGTPGARNSVFTGKQSDDQMITLSSSRLTPDNDGEEDLLVISFSFPGTGNVVSVIIF
                     DENGSEVKRLATNYFIGSEGMLVWDGTASDGSVVRRGIYIIYISSFDDKGKTGKWKKV
                     CSVLR"
     gene            complement(722..1531)
                     /locus_tag="PLG42_07085"
     CDS             complement(722..1531)
                     /locus_tag="PLG42_07085"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOU31010.1"
                     /translation="MLEKIKETIDFLKSRGFNSPDAGIILGTGLGGLTEKITNSIEID
                     YRDIPNFPVSTVKGHEGKLIYGDFGKKKIVAMKGRFHYYEGYGSEQITFPVRVLKYLG
                     IKCLFLSNAAGGLNPDFEIGDIMIITDHINLLPNPLIGQNDDRIGPRFPDMGEAYDSY
                     LINKALMIAQNHGIKVHRGVYLATTGPTFETPAEYRHFRIIGADAVGMSTTPEVITAR
                     HMGLPCFAVSIITDLGVEGKIKYTTHETVIQEAAKTEERMTKLMTDLIASL"
     gene            complement(1494..2594)
                     /gene="lpxK"
                     /locus_tag="PLG42_07090"
     CDS             complement(1494..2594)
                     /gene="lpxK"
                     /locus_tag="PLG42_07090"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOU31011.1"
                     /translation="MAVRKNILLYPISIAYGFITGLRNFLYNSDILKAKEFNIPVICV
                     GNITVGGTGKTPHCEFLINLLKNKFRVAFLSRGYKRKTRGFIIATPSSTTKEIGDEPM
                     QIFMKNPDILVAVDGNRIRGIKNILSQKPDTEVIILDDGFQHRKLKPGLSILLTDYNR
                     PMNHDHLLPFGELRESIKNIYRADIIIISKCPDDITPIQRRIVQREIKKAPYQNLFFT
                     SISYDNPLPVFNADSDSSLLFSQDKSKKYLNVLLVTGIADPEPLAKYLNNKFKEVKHL
                     CFPDHHTYSKKDISRIEEAFRNLPPGEKIILTTEKDAVRLKEMFNIVPDELRAFFYYI
                     PVRINFLNSNEKDFNNLIINYVGKNKRNNRFS"
     gene            complement(2597..4366)
                     /gene="sppA"
                     /locus_tag="PLG42_07095"
     CDS             complement(2597..4366)
                     /gene="sppA"
                     /locus_tag="PLG42_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOU31012.1"
                     /translation="MKNFLIYTLATITGIILSSFIFFLIFIGSLGALIAAGEKPVQLS
                     GKNILVLNAGVPIPDRTNPDPWAGFDILNMSFSPAPGLNDILKNIKKAQHDPAITGIL
                     IENGLLPSGWATATEIRDALLKFRECGKFVLAYSDYVLTQEGYFLSTSADRIYINPES
                     TMEFKGLSGEVMFYKDALEKLGVEVQVVRHGKFKGAVEPYVLNGLSKENREQIEAYVG
                     SIWKYVLGNISAARNIPVDKLNRLADELAAFNPQAAVENGLIDGFLYRDELIDTIKIW
                     SGLKTTDKIGLVSMMKYTKVPDTDKKSFSKNKISVIFAEGTIVTGKGNPTNIGGNYYA
                     DIIREQRKDSTVKAIVLRVNSPGGNAIASEVIWREVALAAASKPLVVSMGNYAASGGY
                     YISAPATRIVASPVTISGSIGVFGLVPNTEKLMKNKLGINTETVNTNKWSDFPSVFRP
                     MSTYEKEVMQKNIENTYNEFTRKVSEGRKIALERVLEIGEGRVWEGVSALNLGLIDEF
                     GGLEKSISVAASLANIKEYSIKEYPEREDPYKRLIAQLTGDVRLKTAIRKFGETARIL
                     EQLNEIQTMTGIQARMPYLIDIH"
     gene            4445..4948
                     /gene="folK"
                     /locus_tag="PLG42_07100"
     CDS             4445..4948
                     /gene="folK"
                     /locus_tag="PLG42_07100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOU31013.1"
                     /translation="MQTAEVVYLGIGSSKGDRPGNLRRATEMIESLIGKIIRSSEVYE
                     TSPWGFESLENFLNMVLIAETFLAPDELLDKIHSIESALGRKRTGNRYSHRVIDIDIL
                     FYGNRVIKTDALTVPHPLLHKRKFVLVPLCSIAPEMVHPVKGKTIRALLDECTDEGMV
                     VLFNQNA"
     gene            complement(4950..5633)
                     /gene="radC"
                     /locus_tag="PLG42_07105"
     CDS             complement(4950..5633)
                     /gene="radC"
                     /locus_tag="PLG42_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOU31014.1"
                     /translation="MSLKITDWAVEDRPREKLYKKGPSALSDAELLGILIGSGTKHRS
                     AVDLGRELLAMAGNNLNNLGKLSIPDIRKIRGIGTARAVAIAAALELGRRRKLAEVPS
                     GIQIKSSKDVFDITEPILADLKHEEFWILFLNRANRVIHQMKLSQGGVSGTVTDVRII
                     MKKALDFLASGIIVCHNHPSGNLTPSDADIKITRKIKEAGELIDIQLLDHLIISEKDY
                     YSFADNGLI"
     gene            complement(5740..5997)
                     /gene="rpsT"
                     /locus_tag="PLG42_07110"
     CDS             complement(5740..5997)
                     /gene="rpsT"
                     /locus_tag="PLG42_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOU31015.1"
                     /translation="MANHKSAEKRARQSLKRKLSNRYYVRTMRTALKRIRTTTSKEEA
                     VAMLPKLTSLIDKMAKKSIIHPNKAANLKSSITKHVNSLTQ"
BASE COUNT         1621 a         1210 c         1270 g         1979 t
ORIGIN      
        1 aagggaagat gtcttctacc tggtgcctat tatgccatca ccaccgacag gaagactgta
       61 gtggagagat atttttcatc ggatgaaaaa aatatttcta atgtttccag atttccttca
      121 atgcctgatg atgaaggtca tttgattctt ttcaataaaa gtctggaggt agttgatgag
      181 gcctattata gtgaaaagca gcattttgcg cttttaactt cagttgaggg ggtttctctt
      241 gaaaaaataa tgccccaggc tgaatcttct tccaggtcga actggtattc ggcatcagag
      301 agttccggat gggggactcc cggagccaga aactctgttt ttaccggaaa acagtcagat
      361 gaccagatga ttactctttc atcttccagg ctaacacccg acaacgatgg ggaagaagat
      421 ctactggtta ttagtttcag tttccccgga actggtaatg tggtcagtgt tattatcttt
      481 gatgaaaacg gcagtgaggt aaagagactt gcaacaaatt attttattgg ctcggaagga
      541 atgcttgttt gggatggtac tgcctctgac ggctcagttg tgagaagggg aatttacatc
      601 atatatattt cgtcgttcga cgataaggga aaaacaggaa agtggaaaaa agtatgttca
      661 gtactcaggt gaaaattcag aacaatttta tctggatgct tatttaagtt ggtatgtaat
      721 tttataatga agcaatcagg tctgtcatta actttgtcat tctttcttct gtttttgcag
      781 cctcctgtat tacagtctca tgagttgtat attttatttt accttcaacg ccgaggtcag
      841 taataatgct tacggcaaag catgggagac ccatatgcct ggcggttata acctcggggg
      901 tggttgacat tccaacagca tcggctccga taattctgaa atgcctgtat tcagcaggtg
      961 tttcgaatgt gggacccgtt gttgcaaggt aaacacccct gtgtactttg atgccatgat
     1021 tttgggctat cataagagct ttgtttatca aatagctgtc gtatgcttct cccatatccg
     1081 ggaaacgcgg gccgattcgg tcgtcatttt gcccgataag aggattggga agcaggttga
     1141 tatggtcagt tataatcatt atgtctccga tttcaaaatc gggattgagc cctccagcag
     1201 cattgctcag aaaaagacac tttattccaa ggtatttgag taccctgacc ggaaaggtaa
     1261 tctgctctga cccgtatccc tcgtaatagt gaaatctgcc tttcatggca acgattttct
     1321 ttttgccgaa gtcaccgtag atgagtttcc cttcatgtcc tttcactgta gaaacaggaa
     1381 agtttggaat gtcgcggtag tcaatttcta tgctgttggt aattttttca gtaagtccac
     1441 caagtccggt tcccagtatt attccggcat cgggcgaatt aaatcctctg cttttaagaa
     1501 aatctattgt ttcttttatt ttttccaaca tagttgatta tcagattatt aaaatctttt
     1561 tcgttgctat ttaaaaagtt aattctaaca ggtatataat aaaaaaatgc tctcagttca
     1621 tcaggaacaa tattaaacat ttctttcaga cgaactgcat ccttttcagt tgtcaggata
     1681 attttttctc cgggagggag atttctgaat gcttcttcaa ttctggaaat atcttttttc
     1741 gaataagtgt ggtgatcagg gaagcagaga tgtttaactt ccttgaactt attattaaga
     1801 tattttgcca gaggttccgg atcggctatt cctgtaacaa gtaaaacgtt cagatatttt
     1861 tttgatttat cctgagagaa caataaagat gagtctgaat cagcattaaa aacgggaaga
     1921 ggattgtcgt aactgataga agtaaagaac agattctgat aaggagcttt ttttatctct
     1981 ctctgaacta ttcttcgttg aataggagtt atatcgtcag ggcacttgct tataattata
     2041 atatcagctc tatatatgtt tttaatactt tctctgagtt ctccgaaagg cagaaggtga
     2101 tcatgattca ttggcctgtt gtaatctgtc agcaatattg aaaggccggg tttaagtttc
     2161 ctgtgctgaa atccgtcatc gagaattata acttctgtat cgggtttctg gctcaggata
     2221 tttttaattc cccttattct gttaccgtca acagcaacaa gaatatcagg atttttcata
     2281 aatatctgca tgggctcatc acctatttct ttggttgttg aagaaggagt ggcgatgata
     2341 aatccccgtg tctttctttt gtaaccgcgg ctgaggaaag caaccctgaa tttgttttta
     2401 agaagattga ttagaaattc gcaatgtggg gtttttcctg ttcctccaac agttatatta
     2461 cccacgcata taacaggaat attaaattct ttagctttga gaatgtcact gttgtacaaa
     2521 aaatttctta acccggttat aaaaccatag gcaatagaaa taggatagag cagtatgttt
     2581 tttcttacgg ccaaagtcag tggatatcta tcagataagg cattcgtgcc tgaattccgg
     2641 tcattgtctg aatttcgttt agttgctcga gaatacgtgc tgtttcacca aactttctta
     2701 ttgcggtttt caacctgaca tctcctgtaa gctgcgcaat gagccttttg taagggtcct
     2761 cacgctccgg atattcttta attgaatatt ccttaatgtt tgcaagtgag gcagcgacac
     2821 tgattgattt ttccagtcct ccgaattcat cgatgaggcc caggttaagc gcacttacac
     2881 cttcccacac tcttccttct cctatttcaa gcacacgttc cagagcgatc tttcgtcctt
     2941 cagagacctt tctggtaaat tcattgtatg tgttttcaat gtttttctgc atcacttctt
     3001 tttcataggt gctcataggc ctgaagaccg aagggaagtc ggaccattta tttgtattga
     3061 ccgtttcagt attgatgccc agtttgtttt tcatcagttt ctcagtgttt ggcacaaggc
     3121 cgaaaacacc aatagaacct gagattgtaa ccgggcttgc cactattctg gtagctggtg
     3181 ctgaaatata atatcctccc gatgccgcat agttgcccat tgagaccact aaaggttttg
     3241 aagcggcggc cagtgctact tcacgccaga tgacttccga tgcaattgca ttaccccccg
     3301 gtgaattcac tctgagaact atagctttta ctgttgaatc tttcctctgt tccctaatta
     3361 tatcggcata ataattgcca ccgatattgg tagggttgcc tttcccggtc actattgttc
     3421 cttcggcaaa aattactgat atcttatttt ttgagaaaga ttttttatca gtgtcaggta
     3481 cttttgtgta tttcatcatt gaaacgaggc ctattttgtc ggttgttttg agccctgacc
     3541 agatctttat tgtgtcaatt agttcatcgc ggtacagaaa gccgtcaata agtccgttct
     3601 ccacagcagc ttgcggattg aatgctgcaa gttcatcagc caatctgtta agtttatcaa
     3661 ccggtatatt tctggcagcc gagatattcc caagaacata tttccaaatt gagcccacat
     3721 aagcttctat ttgctctctg ttttctttac tgaggccgtt cagcacatat ggttcgacag
     3781 cacctttgaa ttttccgtga cgaacaacct gaacctccac ccccagtttt tcgagggcgt
     3841 ctttatagaa cataacctca ccgctgagac ctttaaattc cattgttgat tcaggattaa
     3901 tataaattct gtcggcagat gtcgagagga aatatccttc ctgtgtaaga acgtaatcag
     3961 aataggccag cacaaacttg ccgcattccc tgaattttaa aagagcatcc cttatctcgg
     4021 tggcggttgc ccagcccgaa ggtagcaagc cgttctcaat aagtatacct gtaatggcag
     4081 gatcgtgctg agctttcttt atatttttca ggatatcgtt aagtcccggt gcaggagaaa
     4141 aactcatatt aagtatatca aatccagccc atgggtcggg attggttctg tcgggaattg
     4201 gcactccagc attaagtaca agaatatttt taccgctgag ctgcacaggt ttttcaccgg
     4261 cggcaataag tgcaccgaga ctgccaatga atatcagaaa gaagataaat gagcttaaaa
     4321 taattccggt aattgttgcg agagtataga tcaggaaatt tttcattttg caaaatttta
     4381 tctgaaattt caggcatcta aaaataaata caaataaaac aaaaagataa taattttata
     4441 ctgcatgcaa acggcagaag ttgtatatct cggaattggt tccagcaagg gcgacagacc
     4501 cggtaacctg cgcagggcaa cagaaatgat tgaaagcctt attggaaaga tcattcgctc
     4561 ttcagaagtt tatgagacat ctccatgggg ttttgaaagc ctggagaatt ttctcaacat
     4621 ggtactgatt gcagaaactt ttctggcacc tgatgaatta cttgataaaa tacatagtat
     4681 tgaatcggct ctcggaagaa agagaactgg aaacagatat tctcacaggg tgatagacat
     4741 tgatattttg ttttatggga acagagtgat taagacggat gctctgactg taccgcaccc
     4801 attgcttcat aagagaaaat tcgttctggt gccattatgt tctattgctc cggaaatggt
     4861 acatccggtt aaaggaaaga ccataagggc actccttgat gaatgcactg atgagggaat
     4921 ggtggtgctc tttaatcaaa atgcctgact catattaatc cgttatcggc gaagctataa
     4981 taatcttttt cagatataat gagatgatca agcagctgga tgtctatcag ttcacctgct
     5041 tcctttatct ttctggtaat ctttatgtca gcatcacttg gagtaaggtt tcccgagggg
     5101 tggttatggc agacgattat tcctgaggca aggaagtcga gggctttctt catgattatc
     5161 cttacatcgg taaccgtgcc gctcacgccg ccctggctaa gtttcatctg atgaattacc
     5221 ctgttcgccc ggttgaggaa gagtatccag aactcttcat gtttaaggtc ggcaaggata
     5281 ggttcagtaa tatcaaatac gtcttttgat gactttatct gaattcctga tggcacttct
     5341 gcaagtttcc tccgccgtcc aagttcaagt gcagcagcta ttgccacagc ccttgccgta
     5401 cctatgcccc tgattttcct gatgtcagga atactcagct tgccaaggtt gttgaggtta
     5461 tttcctgcca tggcaagtaa ctcacgtccc agatccacag ccgacctgtg ctttgttccg
     5521 gaaccaatca gaatgccaag tagctcagca tcactcaatg ctgagggacc ttttttgtaa
     5581 agcttttctc tgggtctgtc ctcaacagcc cagtcggtaa ttttcaatga catacagcga
     5641 gacgtttaaa aattttattc cttataaaaa taataatttt aaaataaaaa agccgtcaca
     5701 tttgacggct tttcttaata tttttaatag tcctgatgtt tactgtgtca gactatttac
     5761 atgttttgtt atggaagatt taaggtttgc tgccttgttg gggtggataa tgctcttctt
     5821 tgccatcttg tcgataaggc ttgtcaattt tggcagcatt gctacggctt cttctttgct
     5881 ggttgttgtg cgtatcctct tgagtgccgt gcgcatggta cggacatagt atctgtttga
     5941 cagtttacgt tttaatgact gtcttgctct tttctctgct gacttatggt ttgccatgtt
     6001 aatgttttat atttttcact ttgcattttt gtagtccata ggggaatcga acccctgtta
     6061 ccaggatgaa aacctgacgt
//
LOCUS       DAOSNB010000155         5979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13413_length_5979_cov_3.429271, whole genome shotgun sequence.
ACCESSION   DAOSNB010000155 DAOSNB010000000
VERSION     DAOSNB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13413_length_5979_cov_3.429271"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1601
                     /locus_tag="PLG42_07115"
     CDS             132..1601
                     /locus_tag="PLG42_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOU31016.1"
                     /translation="MLNFKSFLTYGTALAAVVPSLAQKAATIKQSTPNIIIILMDDMG
                     YGDIGRTGANQYETPNLDRLAAQGMQFTWFYCPQAVSSASRAGLLTGCYPNRIGISGA
                     LMPWAEVGINPEETTIAEMLKTKGYYNGIIGKWHLGHHQKFLPLQHGFDEYFGLPYSN
                     DMWPVDYDGVPIRLKDTTSNKMKYPPLPLIEENTKVGEILTLQDQDKLTTLYTERAVK
                     FIEKNKNRPFFLYIAHSMVHVPLGVSDKFRGKSRQGMFGDVMMEVDWSVGEVMKALER
                     NGLEKNTLIIFTSDNGPWLNFGSHAGNTGGLREGKGTSWEGGQRVPCIMKWPGVIPAG
                     TICNKLASSIDILLTLAAITGAPLPDKKIDGVNILPLMRGDKEACPRHEFLYYYQQNA
                     LEAVQIDFWKLILPHSGRTYRGLKPGSDGWPGPTGTEKIESPQLYDLRRDPGEWYDVS
                     QWYPEKVKELMELVERARADLGDDLTKSPGTGRRKPGTL"
     gene            1655..3331
                     /locus_tag="PLG42_07120"
     CDS             1655..3331
                     /locus_tag="PLG42_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOU31017.1"
                     /translation="MKSALKTLNLALAASMALNAFSQQPQKRPNILWISTEDLSPHLG
                     CYGDPVAKTPNIDRLASQGVRYTNVFTTAAISAPCRAGIITGMYQTSIGCMHMRTTSY
                     RRATDNPLEFTAVPPHYVKAFTEYLRAAGYYCTNNVKTDYQFATDPVPASIWDECSRT
                     AHYKNRPDKNQPFFAVFNWTGTHESQNWNLKNVKTDPTRVKVPPYFPDTDTVRLNIAK
                     MYDNIARLDSVVGALLSELEREGEAENTVVFFWGDHGDGLPRAKRWLYDSGLNIPLII
                     RWPGKLKPGIVDDRLISSIDFGPTVLSIAGVTVPAHMQGIPFLGNQTGKPRDAVYAAR
                     DRVDESYDMIRSVRTKNYLYIRNYYPNEPYPIWVAYLSNMPIYREMLRLDADDKLTGA
                     QKAWFAYSRPPEELYDVKADPYQINNLAGDPKFKPVLDEMRSLHEKWTIETGDMGHMN
                     EPEMIEMMWPGGKQPITDMPYFIVNAPEDRSSKNYRTGGTFSSPMTLSFYCPTHGSSI
                     VYTFEENKNPRWLLYTGPLHLKPGTYNIRVKAVRYGYKESEELKGTFVIR"
     gene            3391..4953
                     /locus_tag="PLG42_07125"
     CDS             3391..4953
                     /locus_tag="PLG42_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOU31018.1"
                     /translation="MKSSPHLPLLLSLSSAILSCSSNKDTLQRPNILFIMSDDHAWQA
                     VSAYGGPLKDYAPTPNIDRIAQNGIRFDRCLVTNSISGPSRAVILTGKYSHLNGFLSN
                     EAKEFDGSQQTFPKLLQKAGYTTAIIGKWHLVSNPTGFDHWEILPGQGQYYNPDFITA
                     EGRHREEGYVTEIITEKCIKWLSEVKNSGKPFMLMMHHKAPHREWQPGPNELTLYKDI
                     TFPEPPTLFDDYSGRGRAEREQDMTIAKTMRLQEDLKLYKDPVKGKNVGLNRMTPEQA
                     AAWDSVYNPIIRKFYANPPEGDELVRFKYQRYMQDYLACIAAVDKSVGRILDYLKETG
                     LDKNTIVIYTSDQGFYLGEHGWFDKRFMFEESYRTPLLISWPGVTKSGSVNKNIVSNL
                     DFAETFLDAAGVKIPSDMQGESLVPLLKGKKVPGWRKEHYYHYYEYPAVHSVKRHYGI
                     STERYKLIHFYYDIDEWELFDLQSDPMEMKNVYNDPAYDRVKKQLHKRLEKIMKKYGD
                     SDELALSFLPAK"
     gene            complement(4968..5267)
                     /locus_tag="PLG42_07130"
     CDS             complement(4968..5267)
                     /locus_tag="PLG42_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31019.1"
                     /translation="MHTIYYAGKKPFVTGRLYIHKAECPFLPEERMLLGSFNSVYAAI
                     ESRKFNYGKQEPCPFCCKEQLHFQNREKASTIGKLFVDAFHFAELKVFMPGTYIN"
     gene            5664..5825
                     /locus_tag="PLG42_07135"
     CDS             5664..5825
                     /locus_tag="PLG42_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31020.1"
                     /translation="MKTKNRKPNNELPVFKPEEILSINEMRFVYGGNEDGDGGEDIII
                     IPPPPPKPE"
BASE COUNT         1862 a         1335 c         1256 g         1526 t
ORIGIN      
        1 ctaaccctga gaaaacaaaa gagctttaca aattgctgga cgaatggcgc aaagcaaaca
       61 atgccaggat gatgcttcac aatccaaact ggaaaggcaa atcagaataa acctgataaa
      121 tgtataatat tatgttgaat tttaaatcat tcctgactta tggaacagcc ctggctgcag
      181 ttgttccttc cctggctcag aaagctgcaa ctataaagca atcgacacca aacatcatca
      241 tcattctgat ggatgacatg ggttacggtg atatcggtcg cacaggcgcc aaccagtacg
      301 agacaccaaa cctcgaccgt cttgctgccc aggggatgca gtttacatgg ttctactgcc
      361 cgcaggcagt aagcagtgct tcacgcgccg ggcttctgac aggatgttac ccaaaccgca
      421 taggcatctc cggcgccctg atgccatggg ctgaagttgg cattaatcct gaagaaacaa
      481 ccattgccga aatgcttaaa acaaaaggat attataatgg gattatagga aaatggcatc
      541 tgggtcatca tcaaaagttc cttcctcttc agcatggttt cgacgaatat ttcggactcc
      601 cttattcaaa tgatatgtgg cctgtcgatt atgatggtgt tccgatacgc cttaaggata
      661 ctaccagtaa taagatgaaa tatcctccat taccattgat tgaagagaat acaaaagtcg
      721 gagagatact taccctgcag gatcaggata aactcaccac gttatacaca gaaagagcag
      781 taaaattcat agaaaaaaac aaaaatcggc ctttctttct ctacattgcc cattcgatgg
      841 tgcatgttcc tctgggtgta tctgataagt ttcgcgggaa aagccgtcag ggaatgtttg
      901 gtgatgtaat gatggaagtt gactggtcgg ttggggaagt catgaaagct cttgagcgta
      961 acggtcttga gaaaaacacc ctgattattt tcaccagcga caacgggcca tggctcaact
     1021 ttggcagcca tgccggcaac actggtggct tgcgcgaagg taaaggaaca agctgggagg
     1081 gagggcagcg tgttccctgt ataatgaaat ggccgggagt tatacccgct ggtacaatat
     1141 gtaataagct tgcttcatca atagatattt tgctcactct tgccgctatc accggcgcac
     1201 cgcttcctga taaaaaaatt gatggtgtta acatactgcc tcttatgcgt ggtgataaag
     1261 aggcctgccc gcgtcatgaa tttctctatt attaccagca aaatgcactc gaagcagttc
     1321 agatagattt ctggaaactc attcttccgc acagcggtcg cacttacagg ggtttaaaac
     1381 ccggaagcga cggatggcct ggccctaccg gtacagaaaa gattgaatca cctcaactgt
     1441 acgaccttcg tagagacccg ggtgaatggt acgacgtttc acaatggtac cccgaaaaag
     1501 tgaaggaact tatggaactc gttgagcgtg cccgcgccga cctcggcgac gaccttacta
     1561 agagtcccgg caccggtaga cggaaaccgg gtactttgta ataagttatt ggcaacctgt
     1621 gttcattatg aataaatttt aaatccagaa ttcaatgaaa agtgccctaa aaaccttaaa
     1681 tcttgcactt gcagcttcga tggcgctgaa tgctttcagt caacagcccc agaagcgacc
     1741 aaacatcctt tggatttcaa cagaagattt gagtccacac cttggctgct atggtgaccc
     1801 tgttgctaaa acacctaata ttgaccggct tgcatcacag ggggtgagat atacaaatgt
     1861 gttcacaact gcagctatca gcgccccatg cagagcaggt attataacag ggatgtacca
     1921 gacttcaata ggatgtatgc acatgagaac tacctcatat cgcagggcaa cagataatcc
     1981 attggagttt accgctgttc cccctcatta tgtaaaagca tttactgaat atctgagggc
     2041 tgcaggttat tattgtacta ataatgtcaa aaccgattat cagtttgcaa ccgaccctgt
     2101 tcctgccagt atatgggatg aatgcagccg gacggctcat tacaaaaaca gacccgacaa
     2161 aaaccagcct ttctttgctg ttttcaactg gacaggcact catgaaagcc agaactggaa
     2221 cctgaaaaat gtcaaaacag accctacaag agttaaagtt cctccatact ttcctgatac
     2281 agacactgta aggcttaaca ttgcaaaaat gtacgataac attgcccggc tcgactctgt
     2341 ggttggcgct ctcctgtcag aactggaaag ggaaggggag gcagaaaata cggtggtgtt
     2401 cttctgggga gaccatggcg acggcctccc tcgtgcaaaa agatggttat acgattcagg
     2461 cctcaatatt cctttgatta taagatggcc tggcaaatta aagccaggta ttgtcgacga
     2521 ccgtttgata agctccattg actttggccc gacggtgctt tctatagcag gggtaactgt
     2581 tccggcacac atgcagggga tcccgttcct cggcaaccag acaggtaaac cacgtgatgc
     2641 agtctatgct gcacgcgaca gggttgacga gtcatacgac atgatacgct ccgtacgtac
     2701 aaaaaattat ttgtatatac gtaattatta tccaaacgaa ccgtatccta tatgggttgc
     2761 ttacctcagc aacatgccta tttacaggga gatgcttcga cttgatgccg acgacaaact
     2821 gaccggggca cagaaagcat ggttcgcata ttctcgccct cccgaggagc tctacgacgt
     2881 caaagccgac ccttaccaga taaacaatct ggcgggtgat cctaaattca aacctgtcct
     2941 cgatgaaatg agaagcctcc atgaaaaatg gacaatagag acaggcgata tgggacatat
     3001 gaacgagcct gaaatgattg agatgatgtg gccaggtgga aaacagccta tcacagacat
     3061 gccatatttc atagtgaatg cccctgaaga caggtcttca aaaaactacc gtactggcgg
     3121 tacattctca tcaccaatga cgctttcctt ctattgcccc actcatggct cttcaatagt
     3181 atatacattt gaggaaaaca aaaacccccg ctggctgcta tatacgggcc cgctccacct
     3241 caaacccgga acatacaata tccgtgtaaa ggctgtaagg tacggatata aagaaagcga
     3301 agaactgaaa ggaacgtttg taatacggta aagtaattac aatatctttt aatttatgcg
     3361 aattaataat acttaataga tctctgagtt atgaaatcat caccacacct tccgttatta
     3421 ttaagcctct cgtctgcaat tctttcttgc agcagcaaca aagacaccct gcagcggcct
     3481 aacatccttt tcatcatgtc cgacgaccac gcatggcagg ctgtcagtgc ctatggtggc
     3541 cctttgaagg attatgcccc aacaccgaat atcgaccgta tcgctcaaaa cggaatacgt
     3601 ttcgacagat gccttgtcac aaattctatt tccggtccga gcagagctgt tattctcacc
     3661 ggtaaataca gccacctcaa tgggtttctt tccaacgaag caaaagagtt cgacggctcc
     3721 cagcagactt tcccgaaact cctgcagaaa gcagggtaca ctactgctat aattgggaaa
     3781 tggcaccttg tgtctaatcc tacagggttc gaccactggg aaatactacc cgggcagggc
     3841 caatattaca acccggactt cataacagca gaaggcagac acagggagga ggggtacgta
     3901 actgaaataa ttacagaaaa atgtataaaa tggctctcag aagttaaaaa ctcagggaag
     3961 ccatttatgc ttatgatgca tcacaaagca cctcaccgcg agtggcaacc tggcccaaat
     4021 gaactcacac tttataaaga tatcaccttc cctgaacccc cgaccctttt cgacgactat
     4081 tcaggccgag gaagggcaga gcgtgaacag gatatgacaa tcgcaaaaac aatgcgcttg
     4141 caggaagacc tcaaactata caaagatccg gttaaaggca aaaatgttgg actcaaccgc
     4201 atgacccccg aacaggctgc tgcatgggat tcagtatata acccgattat aagaaagttt
     4261 tatgccaacc cgcctgaggg cgacgaactg gtacggttta aataccagag atatatgcag
     4321 gattatcttg cctgtatagc cgcagtagat aaaagtgtcg gtagaatcct tgattatctg
     4381 aaagaaacag gtctggataa aaatacaatc gttatctata cctccgacca gggcttttat
     4441 ctgggtgaac atggctggtt cgacaaacgc tttatgttcg aggagtctta cagaacacct
     4501 ctcctgatat catggccagg agtaacaaaa tcaggttctg ttaataaaaa tattgtctca
     4561 aatctcgatt ttgcagaaac tttccttgat gctgcaggcg ttaaaatacc ctccgatatg
     4621 cagggcgaaa gcctggtacc tcttcttaag ggtaaaaaag tgccgggatg gagaaaggaa
     4681 cactattacc actattacga atatcctgca gtacatagtg taaaacggca ctacggaata
     4741 agcaccgaaa ggtacaaact gatacatttt tattatgaca tcgacgaatg ggaactcttt
     4801 gatttgcagt ccgatccgat ggagatgaaa aatgtttaca acgacccggc ttacgacaga
     4861 gtgaagaaac agcttcataa acgtctcgag aaaatcatga agaaatatgg cgacagtgac
     4921 gaacttgcac tctcatttct accggcaaaa tgaaaaaaga cctgtgctca gttaatataa
     4981 gttcccggca taaacacctt aagttctgca aaatggaagg catcaacaaa cagttttccg
     5041 attgttgatg ccttttctct attttgaaaa tgcaactgtt ccttgcaaca aaatggacag
     5101 ggctcctgct tgccataatt aaatttgcgg ctctctattg ctgcataaac tgaattgaac
     5161 gacccgagca gcattctttc ctcaggcaga aaagggcact ctgccttgtg aatatacaac
     5221 cggcctgtga caaacggctt ctttccggca taataaattg tatgcatggt taaatttagt
     5281 ttgattcgac attagctcac cagcagttca gggttaagtt tttaccgttt aatatgattt
     5341 acccctgaat tgttactttt ttatatcatt tttttaactg ctaaaattgc tctcctttgc
     5401 tttaattgag aagaagcaac agttaaaaat ttcattgata aatcttttga aatcatctga
     5461 taaagtcact ttgaccagac ctcatttcac ccaataacac ccttaccctg aaagagagtt
     5521 actgtaaggt agattatata acttttttac tcactgataa tcaatatatt attaaatttc
     5581 atgaaaaaat ttttaaaatt taaggtaaca attttgaccc aatcgcaatt aaacggtgtc
     5641 tttaacctta aaaaacagaa gtcatgaaaa caaaaaacag aaaaccaaac aatgaattac
     5701 cggttttcaa accggaagag atactgagca ttaatgagat gaggttcgtc tatggaggaa
     5761 atgaggatgg ggatggggga gaggatataa ttattattcc accaccacct ccaaaaccag
     5821 aatgaactta aaattataca agttaaagca tccctatcgt gaatgctttt ttatttattt
     5881 taataatttt aaccggaaac ttaagtcaat ttcatgtaaa tgataagatt atgtaacatt
     5941 gtttctgtaa aattaaaaaa gccacggctg aaaaaaata
//
LOCUS       DAOSNB010000156         5977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13418_length_5977_cov_4.713610, whole genome shotgun sequence.
ACCESSION   DAOSNB010000156 DAOSNB010000000
VERSION     DAOSNB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13418_length_5977_cov_4.713610"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLG42_07140"
     CDS             <1..312
                     /locus_tag="PLG42_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31021.1"
                     /translation="LLELILSRKLKIHGKLGNPMGRNPSFVGTDSLTEAKEICKILGL
                     KCRNPSFVGTDSLTLIFRMIFNILIYVAILLLLELILSQEEEGQEEEEEKLSQSFFCW
                     N"
     gene            complement(478..966)
                     /gene="cas2"
                     /locus_tag="PLG42_07145"
     CDS             complement(478..966)
                     /gene="cas2"
                     /locus_tag="PLG42_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOU31022.1"
                     /translation="MRRKMPDSPEVSFLKLMERLKKAGLGTRKIVQPESDDAEPLEEL
                     HERIRKILGIFGNLDKKVSKMIYFIMYDIENNKVRNHIAKYLIRKGCTRVQKSIFIAN
                     TERKLYDEIQNTLKKVQDMYDNKDSIFFVPVSVDEIRAMKIIGQSIDFDLVTGSRNTL
                     FF"
     gene            complement(947..2023)
                     /locus_tag="PLG42_07150"
     CDS             complement(947..2023)
                     /locus_tag="PLG42_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type B"
                     /protein_id="HOU31023.1"
                     /translation="MLQIGKNIMLKDDPLTKITVEQLYHIIRKPGTEIETKIRMLKAV
                     KSIDKKKYDILKRDLPYFVCGIFNPPFRKTENFGWINRFILDIDNLTEKGMNVNSLKN
                     KLSTDSRIEMIFVSPGGDGLKLMFCLSEKCYDHGKFSVFYKVFVRSFSGLYGIEQVID
                     PRTSDVTRACFLSYDPEVYYNKVPEPVNMSTFVDFNNPFEVFELQKETKEMEKEFMER
                     EKEINQAAGYSGPDEEILIRIRQTLNPKTRIKNKKIIYVPEELQSTVDKVKEKMSSLS
                     VITDEVIDIHYGKKFRFSVGLRKAEVNLFYGKRGFSVVISPRAGTDNELNTLCGAILK
                     EMFCSEYQKDDTDLKVDRNEKENA"
     gene            complement(2007..3011)
                     /gene="cas1"
                     /locus_tag="PLG42_07155"
     CDS             complement(2007..3011)
                     /gene="cas1"
                     /locus_tag="PLG42_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOU31024.1"
                     /translation="MELVINTFGTSLTRDNENFVVIHKDGKQVIPPAGISSITISRGA
                     QITSDAALLAIENEIEVLFVDNTGNPVGRIWSSKYGSVSTIRKNQIEFTFSPNAVDWI
                     KEIISRKIENQQALLLSLMPEDSSRMAMVKEAINKLEDYRSKVNQLKSDIISDIAPGL
                     RGWEGASSKVYFKTISAFLPEKYCFEGRTKHPATDIVNCLLNYGYGILYGKVESALIK
                     AGIDPYVGIFHRDDYNRPVLAYDVIELFRVWVDYVVISLARQDIITEECYSVRDDNSY
                     WLEALGKRILIQSLNDYFDEIIMIDNLERSRITHINMYAQSLAKMFLNFNPVRNASDR
                     "
     gene            complement(3026..3412)
                     /locus_tag="PLG42_07160"
     CDS             complement(3026..3412)
                     /locus_tag="PLG42_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein"
                     /protein_id="HOU31025.1"
                     /translation="MKEIEHKENKILINLSNHPFTDWDPAQRKESEKYGRIIEIPFPR
                     VDPSASEEEIVRLANETVEKCLIVGKNNELTVHVMGEMTLTYSIVRMLKEKGIKCIAS
                     TSERKALTENGLRLSEFSFVRFREYV"
     gene            complement(3393..4715)
                     /gene="csx2"
                     /locus_tag="PLG42_07165"
     CDS             complement(3393..4715)
                     /gene="csx2"
                     /locus_tag="PLG42_07165"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOU31026.1"
                     /translation="MSRKVLLAFLGTGEYRVCTYKMPSGDTYETNFLLKAVASSVCNN
                     WNENDRIFVFVTGEAKRKNWLNRLNQNGEKIIGLKENLAELKIKAIHEEVEIPEVVSE
                     KNIWDIFEIIFKKLKNQDELYIDITHGFRASPMLLMTLVNYSSFLKKTTIKGIFYGAE
                     QATVEEAGKRITPVWNLNDIALLNEWTSAALEYIKFGDSERIKKLAERSALPGLKQGG
                     QEEKNAALNILNLSKALLKTSRNFTTARGLEICKSETIKEIKNYTEEIRKENLIPPFS
                     PFMDEIESKLNDFEENNVLNFLISVEYCIRHGLVQQGITLLQEGIVTFILKELGYRWC
                     AQTQEERNQVVQNRQDTSNSLNILHEPGKDFKASGSNGDEIYNKIKENKTFSELAKIF
                     SSLSKYRNDINHGGYICPREPENFENELRKLYHKTEKLIKEYNERDRT"
     gene            complement(4782..5933)
                     /gene="csm5"
                     /locus_tag="PLG42_07170"
     CDS             complement(4782..5933)
                     /gene="csm5"
                     /locus_tag="PLG42_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOU31027.1"
                     /translation="METRYFIKATAVTPVHIGAGQEKKMIYGVDYFYDTDENKVIIID
                     QSKAYSMMPPAQKELYINYLAEGKTNMFFNRFENLREEIIKSSAIARINSPSGDPQNN
                     EIIPMISSPGKNGRQVYIPGSSIKGALRSVIYAWLYDKIKRNYKQEEKNYETEFFGKI
                     ENNLMSFIQVSDAPVSGTRIIKTKLYNLRKEGQEIYAGWKNDRFNTSSDFMPAGFLTF
                     LEAIPVGEKFEFSVKINRDRINYLKRQRTVNLPPNLHLIENITLKELFEILNWHSRRY
                     LTAEKDFFNHYSSGEDYSEDIIEEIRRIIQLIPSDNSKCIFHLGFGSGFHGITGDWKF
                     SGDHINTPTSKKEMPKYKSRRMFFRKIKGKTGYSFMPLGFILLEAVAEN"
BASE COUNT         1723 a         1189 c          891 g         2174 t
ORIGIN      
        1 ttgttggaac tgattctctc acggaaactg aaaatacatg ggaagttagg aaatcccatg
       61 ggtcgcaatc cttcttttgt tggaactgat tctctcacag aggcgaaaga aatttgcaaa
      121 attttaggac ttaaatgtcg caatccttct tttgttggaa ctgattctct cacattaatt
      181 ttccgtatga tttttaacat tttaatatat gtcgcaatcc ttcttttgtt ggaactgatt
      241 ctctcacaag aggaagaggg acaagaagag gaagaggaaa aattgtcgca atccttcttt
      301 tgttggaact gattctctca cagtaaccac tgtaatttat tattcttaaa caacttaaat
      361 ttatttttta acctcaattt taactttttc atttcaatag accgcaaaaa tttacaaatt
      421 ttaatgcaaa aaagtgttac taatctaatg tagtattata aaatataaat catttaacta
      481 aaaaaacaaa gtatttcttg agccggtaac cagatcaaaa tcaatactct gccctattat
      541 cttcatcgct cttatttcat caacagatac tggaacgaaa aatatactat ctttattatc
      601 gtacatatcc tgtacttttt tcaaagtatt ctgaatttca tcatataact tcctttcagt
      661 gtttgcaata aaaatcgatt tctgaaccct tgtacatcct ttgcggataa gatattttgc
      721 aatatgattt cttactttat tattttcaat gtcatacata atgaagtata tcattttact
      781 tactttttta tcaagattcc caaaaatgcc cagtatttta cgaatccttt catgtaattc
      841 ttcgagtggt tctgcatcat ctgattcagg ctgaacaatc tttctggtac ccagtccagc
      901 tttttttagc cgttccatca acttcaaaaa tgatacctcc ggtgaatcag gcattttcct
      961 tctcatttct atcaactttt aaatcagtat catctttctg gtattctgaa caaaacattt
     1021 ccttcagaat tgcaccacac aaagtattaa gttcattatc tgttccggct cttggtgata
     1081 tcaccaccga aaaacctctt ttaccgtaaa aaaggttaac ctctgccttt cttaaaccaa
     1141 ccgaaaacct gaatttctta ccatagtgta tatctataac ctcatcggta ataacagaaa
     1201 gcgatgacat tttttctttt actttatcaa ctgtcgactg tagctcttca ggcacatata
     1261 taatcttttt attctttatt cttgttttgg gatttaatgt ttgccttatt ctgattagaa
     1321 tctcttcatc gggtccacta tagcctgctg cctggtttat ctctttttcc ctttccatga
     1381 actccttttc catttctttt gtttctttct gaagctcaaa tacttcaaat ggattattga
     1441 aatcaacaaa agtactcatg ttcacaggct caggaacctt gttataataa acttcagggt
     1501 cgtaacttaa aaaacaggct ctggttacat cactggtacg aggatctatt acctgttcaa
     1561 ttccatataa ccctgaaaat gaccttacaa aaactttata aaaaactgaa aacttcccat
     1621 gatcgtaaca tttttccgaa agacagaaca ttaacttcag cccgtcaccc ccgggggaaa
     1681 caaagatcat ttcaatcctg ctatcagtgg aaagtttatt ttttaatgaa ttaacattca
     1741 tacctttttc tgtcagatta tcaatatcga gtataaatct gtttatccat ccgaaattct
     1801 ccgttttcct gaaaggtgga ttaaaaatac cgcatacaaa ataaggtaag tcccttttta
     1861 aaatatcata tttctttttg tcaatagatt taactgcctt aagcattctt attttagttt
     1921 ctatttcagt accaggttta cgaataatat gataaagctg ttcaaccgtt atctttgtaa
     1981 gcgggtcatc cttcaacatt atatttttac ctatctgaag catttcttac aggattaaag
     2041 ttcagaaaca tttttgcaag tgattgagca tacatattaa tatgtgtaat tcgtgacctt
     2101 tcaaggttat ctatcattat tatttcatcg aaataatcat ttagtgattg gataagtatt
     2161 cttttaccca atgcttcaag ccaataagaa ttatcgtccc ttacactgta acattcttcg
     2221 gtaattatat cctgcctggc aagagatatt acaacatagt caacccagac cctgaaaagt
     2281 tctattacat catatgcaag tactggcctg ttatagtcgt cgcggtgaaa aatacccaca
     2341 taaggatcga tgccagcttt aataagcgcg ctctccacct tcccgtacaa tattccatat
     2401 ccataattta aaagacagtt aactatatcg gttgcagggt gttttgttct gccttcaaaa
     2461 cagtactttt cgggtagaaa agctgaaata gttttgaaat agactttaga tgatgcaccc
     2521 tcccatcctc ttaatccggg cgcaatatca cttataatat cacttttaag ctggtttact
     2581 ttcgaccgat aatcttcaag tttatttata gcttctttaa ccattgccat tctggagcta
     2641 tcttctggca tcagtgaaag aagaagtgcc tgctggtttt ctatttttct ggatattatc
     2701 tccttgatcc agtctactgc attgggtgag aatgtaaact caatctggtt tttccttata
     2761 gtagatacag aaccatattt tgaactccat atcctgccaa caggatttcc cgtgttatca
     2821 acgaacagaa cttctatttc attttcaatg gcaagcaaag cagcatcact tgttatctgg
     2881 gctcctcttg agattgtaat agaactaatt ccagcaggtg gaataacctg tttaccatct
     2941 ttatgaatca caacaaagtt ttcattgtcg cgtgtaagtg aagtaccaaa agtattgata
     3001 accagctcca tagtcaatta ctatactata catattccct gaaacgtaca aacgaaaatt
     3061 cggataatct caatccattt tctgttaacg ccttcctttc tgaagttgaa gctatacatt
     3121 ttattccttt ctcttttaac atccttacaa tgctatatgt gagagtcatt tcacccatga
     3181 catgtacagt cagttcatta ttcttcccga ctataaggca tttttcaaca gtctcatttg
     3241 ccagcctgac aatttcttcc tcactggcag aaggatctac acgagggaac ggtatttcaa
     3301 ttattctccc atatttctcc gattctttcc tctgagcagg atcccagtca gtaaatggat
     3361 ggtttgaaag atttatcaaa attttattct ccttatgttc tatctctttc attgtattct
     3421 ttaatgagtt tttcggtttt atggtaaagc tttcttaatt cattttcaaa attttccggt
     3481 tctctggggc aaatatatcc accatggtta atgtcatttc tatattttga aagtgagctg
     3541 aatatctttg caagttcact aaaagtctta ttctctttta ttttattata tatttcatct
     3601 ccgttacttc cacttgcttt gaaatcttta cctggttcat gtagaatatt cagactgttt
     3661 gaagtatcct gcctgttttg aactacctga tttctttctt cctgggtttg agcacaccat
     3721 ctataaccta actccttcag gataaatgtt acaatacctt cctgaagcaa tgtgataccc
     3781 tgctgtacca gaccgtgccg tatgcagtat tcaaccgaaa taagaaagtt aagcacatta
     3841 ttttcttcaa aatcattgag ttttgattca atttcgtcca taaatgggct gaatggaggg
     3901 attagatttt cctttctgat ttcctctgta taatttttta tttcctttat ggtttccgat
     3961 ttacatattt caagacctct tgcagtggta aaattccggg aagttttaag taatgctttt
     4021 gacagattaa gaatattaag ggcagcattt ttttcttcct ggccaccttg cttaagccct
     4081 ggaagagcac ttctttcagc aagctttttg attctttctg aatcaccaaa cttaatatac
     4141 tctaaagctg cactggtcca ctcattaaga agtgcaatat cgttaaggtt ccagacagga
     4201 gtaattcttt ttccagcctc ttctactgtt gcctgctcgg caccgtaaaa aattcctttg
     4261 attgttgttt tctttagaaa tgaggaataa tttacaagag tcatgaggag cattgggctt
     4321 gctctgaagc cgtgtgtaat atctatgtag agctcatcct ggtttttaag ttttttaaat
     4381 attatttcaa aaatatccca tatatttttt tcagaaacaa cctcaggaat ttcaacctct
     4441 tcatgaatcg ctttaatttt cagttcagcc agattttctt ttaatccaat gattttttca
     4501 ccgttctgat taagacggtt taaccagttt tttctctttg cttcgccagt aacaaataca
     4561 aaaatcctgt cattttcatt ccaattattg cacacagatg atgctaccgc tttaagtaga
     4621 aaatttgttt cgtatgtgtc gcctgaaggc attttgtatg tacaaaccct atattcgcct
     4681 gtacccaaaa atgccagaag aacttttcta ctcatatttt atatgattta aaagctatta
     4741 ataaccgcag ccagcagcca ggtccgttaa tttaatatgg ttcaattttc tgctacagcc
     4801 tcgagcagaa taaagccaag cggcataaaa ctgtaacctg tcttaccttt gatttttcta
     4861 aaaaacattc ttcgtgattt atacttcggc atttcctttt ttgaagtagg cgtattaata
     4921 tggtcacccg aaaatttcca gtctcctgta attccgtgaa atcctgagcc aaatcccaga
     4981 tgaaaaatgc atttactatt atcagatggt atcagttgaa tgatacgtct aatctcctca
     5041 ataatatctt ctgaataatc ttcaccactg ctataatgat taaagaaatc tttttctgcc
     5101 gtcaggtatc ttcgggaatg ccagtttaaa atttcgaaca attccttcag agtgatattc
     5161 tcaattaaat gaagattagg gggtaaatta acggttcttt gtctttttaa ataattgatt
     5221 ctgtctctat ttatttttac tgaaaactca aatttttcac ccacaggtat agcttccaga
     5281 aaggttaaga atcctgcagg catgaaatca gaagaagtat taaaacggtc gtttttccag
     5341 ccggcataaa tttcctgacc ttcttttctt aaattgtata gtttagtttt gataattctt
     5401 gtacctgaca ccggggcatc tgaaacttga ataaatgaca taaggttgtt ttcaattttt
     5461 ccaaaaaatt ctgtttcata atttttttct tcctgcttat agtttctctt aattttatca
     5521 taaagccagg catatattac cgatcgcaat gcacctttaa ttgatgaacc aggtatataa
     5581 acctgcctgc cgtttttgcc cggcgatgat atcataggaa ttatctcgtt attttgcggg
     5641 tctcctgatg gggagtttat tcttgcgatt gctgatgatt taattatctc ttcacggagg
     5701 ttttcgaacc tgttaaaaaa catgttggtt tttccttctg ccaggtaatt aatatacaat
     5761 tctttctggg caggcggcat catgctatag gcttttgact ggtcgataat gattacctta
     5821 ttttcatcag tatcataaaa ataatctact ccgtatatca ttttcttttc ctggcctgca
     5881 ccaatatgaa ccggcgttac agcagttgct ttaataaaat atcttgtttc catttcatta
     5941 tggttttacg ggtaagaata tgcttctgcc gcttctg
//
LOCUS       DAOSNB010000157         5966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13449_length_5966_cov_3.737439, whole genome shotgun sequence.
ACCESSION   DAOSNB010000157 DAOSNB010000000
VERSION     DAOSNB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13449_length_5966_cov_3.737439"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLG42_07175"
     CDS             <1..490
                     /locus_tag="PLG42_07175"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOU31028.1"
                     /translation="IVALTFPVMGIFSWLNRKGIEPSTPKKIGFGMIIAATGFMVVLI
                     ASVNLASPKLLAGTPLPDSDRVSPYWLISSYLILTIAELFLSPMGLSFVSKVAPSRFQ
                     GLMQGGWLLATAVGNKLLFVGTLLWDRVELWQLWLVFIVCCLASAAFIFSILKRLESA
                     SK"
     gene            511..1545
                     /locus_tag="PLG42_07180"
     CDS             511..1545
                     /locus_tag="PLG42_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31029.1"
                     /translation="MKEKLPAGGTKRRNIIKGIFAGIPFLTFFSGSPFRIPAEKKPGS
                     TEGETSKEEPSGQQNILPSGKIAGYNVSRLILGCNLISGYAHARDLRYADTLFKVYHS
                     EEKVIETYRLAEAAGINAIFVVNRNFHLFNKYREKYGSKMLTINQTYLRDDNFLGDID
                     LAVSNGATMLYIQGAESDRYVRDGKTEMLGRAIEYIKKHGKPAGIGAHSIEVIKACEK
                     EGIPADFYVKSFHHDRYWSAHPVENRTEFSIIGSQRSTDHNMIHDNIFDLFPDKTAEF
                     MKEVRKPWIAFKVLAAGAILPQDGFRYAFENGADFICVGMFDFQIAEDAKIAAEILNS
                     LKNRTRNWYS"
     gene            complement(1592..2437)
                     /locus_tag="PLG42_07185"
     CDS             complement(1592..2437)
                     /locus_tag="PLG42_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31030.1"
                     /translation="MRHIYLVIILCTISVMVAKAQPYNFPPLKGYKIVSKYPVYEPDN
                     LWDFIDGAADGYISYGFQNVHVREYVKGKNVIKVEIYRHKDRDNTFGIYASERSPSYR
                     YINLGAQGYVAGGVINFFKDCYYVKLRTFTENEKVLRDLESLAYRVADMLPGDPSMPR
                     TLNEFPQNFKEKNTETYINESVLGHDFLKGAYRADYKQEGITYQVYIFDKNSPEDAFQ
                     TVKSYLALAGLEPDDSAEGKYVFKDGYNGNIFLARKENRIVIITGLEKDQTNIADRIT
                     GEILR"
     gene            complement(2455..3657)
                     /locus_tag="PLG42_07190"
     CDS             complement(2455..3657)
                     /locus_tag="PLG42_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU31031.1"
                     /translation="MRKMKNNINRRKFLSTGITGFTGAMVLSSPFAKGQTSEQEKKIV
                     YRTLGKTGIKVPVISFGVMRADSPALCKAAYDNGINFFDTANVYQGGNNETMLGNFFK
                     NLPRSSFIVETKVKPAGVGRDGLPTAQTTAEDFLGKFKESLSRLQLDYVDILLIHDVS
                     NPELLKHKPLISALTQLKKEKKTRFVGFSTHSNMAVMLQEAANSDFWDVVLTSFNFML
                     NNIDEMNQAIKKAADAGIGIVVMKSLAGGFMDKERKKPVNATLALKWVLSNPNVHTII
                     PGMTSFDHLETNLKVLADPVMTEQEKQELLAMAGEPGLFCVNCNKCLNACPYNLPVPD
                     LMRAYMYAYGYANLRMAYDLLGELGTGVSPCINCTECKVQCTKGFDIRTKIADVSRLV
                     SMPADLIA"
     gene            3826..4512
                     /locus_tag="PLG42_07195"
     CDS             3826..4512
                     /locus_tag="PLG42_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU31032.1"
                     /translation="MNNYLRKLISGGENQRVDFKYCINDSKKIARTLSAFANTEGGIL
                     LLGVRDNGSIAGVDSEEEYYMIDAAAKLYCRPEIPVRIKQHYINGKTVIEVEVKKGTH
                     RPYLAKGDDGRWKAYFRNNDQNLVANRILLKLWKKDERKSGVLIRFGKAENMLMEYLK
                     NNDSISLSSFRKLANIPAYRAEKIIVNMLLCGVLIMKASEKGFRYALNRNPGETDLKT
                     DLKTDLKENR"
     gene            complement(4518..>5966)
                     /locus_tag="PLG42_07200"
     CDS             complement(4518..>5966)
                     /locus_tag="PLG42_07200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU31033.1"
                     /translation="KQIDDKIWMPKSKNIYVIDKEKLKAPAPKINTLLTKIVIGKDSL
                     FMSGTFYKTLGDGKRIPAVNNSANPVPEIKYNFNSVSFYWSVPYYVDEDKNLYSYKLD
                     GFDRSWSKWENIHYKDFTNLPFGKYTFRVKGKTTTDIETEEAVFDFIILKPWYLTTVM
                     IILYAIAFVLLIIGIIKAYTRKLKNENIRLEGIIAERTAVVVKQKEELESSIHYARRI
                     QMALLPSESILAENLKNYFILFKPRDIVSGDFYWMTKKDNRLYIVAADCTGHGVPGAF
                     MSLLGMSFLDEIIGGEKAPRADEVLSELRLHVTESLKQSGGDDEAKDGMDMALLVIDF
                     NRSRVEFSGAYNPCFRVRRLTEKEQKDYHEDNIEMPDGSMTNGVYILETIFASKMPIG
                     ISSKMDEKFVFYDWELEKGVSYYLFSDGYIDQFGGPHGRKFMKKNFKRLILEIQDFPM
                     KRQKEILEERLKEWMGQTPQIDDILVMGIRTD"
BASE COUNT         1738 a         1189 c         1164 g         1875 t
ORIGIN      
        1 tattgttgct ctcaccttcc ctgtgatggg aattttttcg tggcttaaca gaaaaggcat
       61 tgaaccttcc actcctaaaa agattggttt cgggatgatt attgctgcta caggttttat
      121 ggttgttctc attgcatcgg tcaatcttgc ttcacccaaa cttcttgcag gcactcccct
      181 tcccgacagt gacagggtat cgccttactg gcttataagt agttatctta ttcttaccat
      241 tgctgaatta tttctgagtc cgatggggct ttcatttgta tcaaaagttg ctccctcgag
      301 atttcagggt ctcatgcagg gcggatggct tcttgctaca gctgtcggaa acaaacttct
      361 ctttgttggt acgcttctct gggacagggt tgagctatgg caactgtggc tggtatttat
      421 agtatgctgt cttgcttctg ccgcatttat cttttcaatt ctaaagcgcc ttgagagcgc
      481 atcaaaatga aaacctttaa aaacaaaaac atgaaagaga aattgcctgc agggggaaca
      541 aagagacgaa atataattaa aggcatcttt gcaggcattc cttttttaac atttttctcc
      601 ggttcaccgt tcagaatacc ggcagaaaaa aaacctggct ctactgaagg ggagacgtca
      661 aaggaagaac catccggcca gcagaatatt ttaccttccg gaaaaattgc aggttataat
      721 gttagccgac ttattctggg gtgcaactta ataagcggtt acgctcatgc aagggacctc
      781 aggtatgccg atacactttt caaagtctac cattcggaag agaaagttat tgagacatac
      841 cgtcttgctg aagctgcagg aataaacgcc atttttgttg tcaatcgcaa ttttcatctc
      901 ttcaataaat acagagagaa atatggcagt aaaatgctga caattaatca aacatacctg
      961 cgtgatgata atttcctggg cgacatagat ctggcggttt caaacggcgc taccatgctg
     1021 tacatacagg gtgccgagag cgacagatat gtaagggacg gaaaaactga aatgcttggc
     1081 agggcaattg aatatataaa gaaacatggc aaacctgctg gtataggagc gcattcgata
     1141 gaagtaatca aagcatgtga aaaagaaggt attccagctg atttttatgt gaaatcattc
     1201 catcacgaca ggtactggtc tgcccatccg gtggaaaaca ggactgaatt cagtataata
     1261 ggatcacaga gaagcactga ccataacatg atccatgaca acatttttga cttgtttccg
     1321 gataagacag ctgaattcat gaaagaagta agaaagccat ggattgcttt taaagtgctt
     1381 gcggcaggtg ccatattacc tcaggatgga ttcaggtatg cattcgaaaa cggtgcagat
     1441 ttcatctgtg tgggcatgtt cgactttcag atagctgaag acgcgaaaat tgctgctgaa
     1501 atacttaaca gcctgaaaaa tagaaccagg aactggtatt cataacacag ttcccggttt
     1561 tcataatttc ctgattaatt tattcagtat attatctaag aatttcccct gtaatgcggt
     1621 cagcaatatt tgtctggtct ttttcaaggc cggtaatgat tacaattctg ttttccttcc
     1681 tggcaaggaa aatattgcca ttgtatccat ctttaaaaac atattttcct tcggcagaat
     1741 catcaggttc aagcccggca agtgccaggt acgatttaac tgtttgaaat gcatcctctg
     1801 gtgaattctt atcaaaaata tatacctggt atgttattcc ttcctgctta tagtctgccc
     1861 tgtatgctcc tttaaggaaa tcatggccta ggacactttc gttaatgtat gtttcggtat
     1921 ttttctcttt gaagttttga gggaactcat ttaatgtccg gggcatcgac ggatctcctg
     1981 gtaacatatc cgcaactctg tatgccagag attcgaggtc tctcaatact ttttcatttt
     2041 cggtaaaggt tctcagttta acgtaataac aatctttgaa gaagtttatc actcctccag
     2101 ccacataacc ctgagctcca aggttaatat atctgtacga tggagaacgt tccgacgcat
     2161 agattccgaa agtgttgtca cggtctttat gccggtaaat ctcaaccttt ataacatttt
     2221 ttcctttgac atattccctg acgtgcacat tctgaaaacc gtatgaaata taaccatctg
     2281 ctgcaccgtc aataaaatcc cagaggttat caggttcata caccggatat tttgagacga
     2341 ttttatatcc tttcagtggt ggaaaattat atggctgagc ctttgccacc atgactgaaa
     2401 tcgtacacag gataatcacc agatatatat gcctcataat caatatttta catttcaggc
     2461 aataagatct gccggcatac ttacaagtcg tgaaacatca gcaatttttg ttctaatatc
     2521 gaatcctttt gtacactgaa ctttgcattc ggtgcagttt atgcatggtg atacgcctgt
     2581 acccagttca cccagaaggt cataagccat tcgaagattt gcatagccgt atgcatacat
     2641 atatgctctc ataaggtcag gaactggcag gttatatggg catgcattaa ggcatttatt
     2701 gcagtttaca cagaatagtc cgggttcacc tgccatagca agaagttcct gtttctcctg
     2761 ctcggtcatt accgggtcgg caagtacttt aagatttgtt tcaagatggt caaatgatgt
     2821 cataccaggt ataatggtat ggacattggg atttgaaagc acccatttta gagcaagtgt
     2881 agcatttacg ggtttcttcc tttctttgtc cataaaccca ccggccagag acttcattac
     2941 aacaattccg ataccggcat cagcagcttt tttaattgct tgattcattt catcaatatt
     3001 attcaacata aaattaaagg aagttagaac gacatcccag aaatcggaat tagctgcttc
     3061 ctggagcata acagccatat ttgaatgggt actgaatcct acaaaccttg ttttcttttc
     3121 ttttttcagt tgtgtcaatg ctgaaatcaa aggtttatgt ttgagaagtt cgggattgct
     3181 tacatcatga atcaggagaa tatcaacata atcgagttgc aatcttgata aactttcctt
     3241 gaatttcccg aggaaatcct ctgcagttgt ctgagcggtt ggaagtccgt ctctgcccac
     3301 tcctgcaggt tttacttttg tttcaacaat gaaggatgaa cgtggcaggt tcttaaaaaa
     3361 atttccaagc attgtttcgt tattaccacc ctggtaaacg ttggcggtgt caaaaaagtt
     3421 tatgccgtta tcatatgctg ctttgcaaag cgcaggacta tcggctctca tgacaccgaa
     3481 acttattact ggaactttaa tacctgtctt accaagtgtg cggtatacaa tctttttttc
     3541 ttgctctgag gtctgtccct ttgcaaaagg gcttgacaaa accatcgcac cagtaaaacc
     3601 tgttattcca gtgctaagga attttctcct attgatattg tttttcattt tcctcataaa
     3661 taataatttt ggaaggtcaa atatataagt ttttacatta aattcgaatg gagaaaaaca
     3721 ataaatgtca aaatataaca tcttttaaca tcgttatatt ttatggctca tttccaggaa
     3781 ggatttttgt tttttgtgag ttgattttca taaaaataac cgtctatgaa caattacctg
     3841 agaaaattaa tctctggcgg tgaaaatcag agggtcgatt tcaaatattg cataaatgat
     3901 tcaaaaaaaa tagcgagaac tttatcggca tttgccaata ctgaaggtgg tattttactg
     3961 cttggggtta gggataatgg ctcaattgcc ggtgttgatt ctgaagaaga gtattacatg
     4021 atcgacgctg cggccaaact ttattgcagg cctgaaattc ctgtcagaat aaaacaacat
     4081 tacatcaatg gcaaaactgt aattgaggtt gaagtaaaaa agggaacaca tcgtccttac
     4141 ctggccaaag gcgatgatgg aaggtggaaa gcatatttca gaaataatga tcagaatctt
     4201 gttgcaaatc gaatactgct taaattatgg aagaaagatg agagaaaatc gggggtactt
     4261 ataagattcg gtaaagcaga gaatatgctt atggagtatc ttaaaaacaa tgattccatc
     4321 agcctttctt cgttcagaaa gctggcaaat ataccagctt acagggctga aaaaattatt
     4381 gtcaatatgc ttctttgcgg tgtgctgata atgaaggcat cagaaaaagg gttcaggtac
     4441 gcacttaatc gtaaccccgg tgaaacagat ttaaaaacag atttaaaaac agatttaaaa
     4501 gagaatagat aattttttca gtcggttcgg attcccataa ccagaatatc atcaatctga
     4561 ggtgtctgtc ccatccattc cttaagtctc tcttcaagta tttccttctg acgtttcatg
     4621 gggaagtcct gtatttcgag aataagtctt ttgaaattct tcttcatgaa tttacgtcca
     4681 tggggccctc cgaactggtc aatatagcca tctgaaaata aatagtatga gacgcctttc
     4741 tcaagttccc aatcatagaa cacaaacttc tcatccattt ttgaagaaat accgatgggc
     4801 attttgctgg cgaatattgt ttcaaggata tagacgccat tagtcataga gccgtcgggc
     4861 atttcaatat tatcttcgtg ataatccttc tgctcctttt ctgtgagtct tcttactctg
     4921 aagcaaggat tgtatgcccc cgaaaattcg actctcgacc tgttgaaatc tatgaccagc
     4981 agggccatat ccataccgtc ctttgcctca tcatcgcctc ccgactgctt tagagattca
     5041 gttacatgca atctcagctc actaagtacc tcatcagcgc gtggggcttt ctcccctcct
     5101 ataatctcat caagaaacga cattcccagg aggctcataa atgcacctgg cactccgtga
     5161 cctgtacagt cagctgccac tatatataat ctgttgtctt tttttgtcat ccagtagaaa
     5221 tcaccactta caatatctcg cggtttgaaa agaatgaagt aatttttcag gttttctgca
     5281 aggattgact cagaaggaag cagtgccatc tgaattcttc tggcatagtg tatactcgat
     5341 tcaagttcct ccttctgctt tacaacaact gccgttcttt cggcaataat gccctcaagt
     5401 ctgatatttt cgtttttcag tttccgggta taagctttaa taattccgat aatcagaagt
     5461 acaaaggcaa ttgcatacag gattatcatc accgtggtga gataccacgg cttaagaata
     5521 atgaaatcaa aaactgcttc ttccgtttca atatcagtag tcgttttacc ctttaccctg
     5581 aaagtgtatt ttccaaaagg aaggtttgta aaatctttat aatgaatgtt ttcccatttt
     5641 gaccatgatc tgtcgaatcc atcaagtttg tagctgtata agttcttatc ctcatctaca
     5701 taataaggga ctgaccagta aaaagatact gaattgaaat tgtattttat ttcaggtaca
     5761 gggttggcgc tgttattaac tgcgggaata cgtttaccgt caccaagagt tttataaaat
     5821 gtacccgaca taaacagtga atcttttcca ataactattt ttgttagaag ggtgtttatt
     5881 tttggtgcag gtgctttaag tttttcttta tcaattacat aaatattctt tgatttgggc
     5941 atccatatct tgtcatcaat ctgttt
//
LOCUS       DAOSNB010000158         5956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13482_length_5956_cov_4.931537, whole genome shotgun sequence.
ACCESSION   DAOSNB010000158 DAOSNB010000000
VERSION     DAOSNB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13482_length_5956_cov_4.931537"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PLG42_07205"
     CDS             <1..1360
                     /locus_tag="PLG42_07205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU31034.1"
                     /translation="FTGIEALKNASFRDTEGKLWFGTANGVTMLNPVKLPPPGTPPAV
                     YISGMEVNNKPREMKAGLKLSYREKSVSFSYYSISLFDPKSVRYSVMLEGADPDWRPA
                     TSSTSFNSVLPPGHYKFKVKAINSSGYWSDPPAEFSFIVKPPFYQSPLFIISIIILSV
                     IAVIFYVKIRERNLIREKRILEAKVAERTAELVQKNQIIEEKNRDITASIRYAERIQR
                     AMLPREDIFEETFVLFLPKDIVSGDFYWMYDTGDVRLIAAVDCTGHGVPGAFMSIIGH
                     NSLNKVVREYGLIRPSAILDQLNIEVSKSIIQSHEKGITDGMDLALIAYNRKKFTLEF
                     AGAYNPLYLVRNGEVIVYKGDRFPIGMVTIEQKKKFTNVNVDIQPGDMIYMCSDGYAD
                     QFGQAENKKFKVGNIKRLLSEIYNLPVNEQKARLEKEIMDWKGDLPQVDDILFIGMRV
                     VP"
     gene            complement(1357..1935)
                     /locus_tag="PLG42_07210"
     CDS             complement(1357..1935)
                     /locus_tag="PLG42_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOU31035.1"
                     /translation="MRVCVFAASSNRVDERYYEAAGELGRMLAASEMEVVFGGGGIGL
                     MGRLADAVLNNNGKITGVIPRFMQDEGWGHSSVKEMIVTEGMPQRKQLMMQMADAIVA
                     LPGGIGTLEELAEAITLKQLGLFKGPIVILNTLNFYDQFLNFLEEMVRKNFMRKEHGE
                     IWNVVNYPSEVIDSIRNYKGWYDNPRSFAKID"
     gene            complement(1984..2301)
                     /gene="trxA"
                     /locus_tag="PLG42_07215"
     CDS             complement(1984..2301)
                     /gene="trxA"
                     /locus_tag="PLG42_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOU31036.1"
                     /translation="MVLEVTDSNFEEIVLKSEKPVIVDFWAEWCGPCRRIAPFIEEIS
                     KEYEGKVVVAKCNVDNNPKVVKEMNVRNIPAVFYFKNGKEVDRQIGAVAKKIFTDKLE
                     KLL"
     gene            complement(2352..5840)
                     /gene="dnaE"
                     /locus_tag="PLG42_07220"
     CDS             complement(2352..5840)
                     /gene="dnaE"
                     /locus_tag="PLG42_07220"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU31037.1"
                     /translation="MALFSHLHVHTQYSILDGASSIPALVDRAKSLGMDALAITDHGN
                     MFGVKEFHNYAVSKGIKPVLGCEIYIAKGSMEEKTGSEDRSGDHLILLAKNLTGYRNL
                     IKLVSLAWTKGFYYTPRIDKKALEKHRDGLIATSACIAGEIPGEILNGNIEGAEKALK
                     SYIDIFGEDFFLEIQRHETTEPDANIETFPKQQKVIEAFRRLSEKYGVKIIATNDVHF
                     ANKEDADAHDRLICINTAKDLDDPERMRYSRQEYLKSEEEMREIFSDIPEAIENVAEI
                     ISKIELYELDHDPIMPEFKIPPEFSSESEYLRYLTYEGAKERWGVLTEEQKERLEFEL
                     DTIIRMGFPAYFLIVHDFLKAAREMGVAVGPGRGSAAGSAVAYCLRITDIDPIKYGLL
                     FERFLNPDRISLPDIDIDFDEDGREQVLRYVVNKYGHDRVAHIITFGTMGAKMAIRDV
                     ARVQKLPLSEADRLAKMVPERPGITLADAYAEVPELAKEKDSPNKLISQTLQFAEALE
                     GSIRQTGVHACGIIISKEPLDNYIPLSTAKETDLFVTQYEGNHVESVGLLKMDFLGLK
                     TLSIIRDAIENIKKRHGIEIDIENLPLDDKKTFELFSRGDTTGIFQFESDGMKRYLRE
                     LKPNRFEDLIAMNALYRPGPMENIPLYIKRKHGQEKIEYLLPEMEKYLADTYGITVYQ
                     EQVMLLSQELAGFTKGEADSLRKAMGKKKKALMEEMKARFMEGCLKKGYSEQLINKIW
                     SDWESFAHYAFNKSHSTCYALVAFQTGYLKAHYPAEYMAAVLSRNISDLKKITLFIDE
                     ARRMGIEVLGPDINESDVKFTVNKDGNIRFGLGAIKGVGENAVMQVIEEREKNGPYRD
                     IYDFVSRVNLNTLNRKNIEAMALAGAFDCFPEISREQYFAADAKGTTFIEHLIRFGNN
                     IKTIKNSSKNTLFGEDNGFSMKKPEPPSCDEWPKLEKLNREKEVIGFYLSSHPLDDFR
                     LEIETFTNTTLEELRNNMQEFIERDVAVAGMVTETRNGISKNNKPYASFTLQDYTDSF
                     RFVLFDKDCVEFSKYLVNGYFLLVKGKVQRRKYAENELDFRVKSITLLSSVKDELIKS
                     VTIKVKAPDVDNELINQLTKMIKANPGETELRFLFIDPESKISLPMFSRTIKVRLNND
                     FITFLEDHPALDFKVN"
BASE COUNT         1586 a         1319 c         1210 g         1841 t
ORIGIN      
        1 tttcacggga attgaagcat taaagaatgc ctcattcagg gacactgagg ggaagctgtg
       61 gtttggaacg gcaaacggag ttacaatgct taaccctgtg aaattaccac ctcccggcac
      121 acctccggca gtatacatat cgggcatgga agtcaacaat aagcccaggg aaatgaaagc
      181 agggctgaag ctcagttaca gggagaaatc ggtcagtttt tcctattaca gtatcagcct
      241 cttcgatccc aaatcagtca gatacagcgt aatgcttgaa ggggccgacc cggactggcg
      301 gccagcaacc agcagcactt ctttcaattc ggttctgcca cccggacatt ataaattcaa
      361 agtaaaagct ataaacagtt ccggatactg gagcgaccca ccagctgaat tctcttttat
      421 tgtaaaacca cctttctacc aatcgccact atttatcatc tcaataatca tcctttcggt
      481 aatagccgtg attttctatg ttaaaatcag agagcgcaac ctgataaggg agaagagaat
      541 acttgaagcc aaagttgctg aaagaacggc tgaacttgtg cagaaaaatc aaattattga
      601 agaaaagaac cgtgatatta cagcaagtat cagatatgct gaaaggatac agagagccat
      661 gctcccgagg gaagatattt ttgaggagac atttgtactg ttccttccca aggatattgt
      721 aagtggtgat ttctactgga tgtatgatac aggtgatgtg aggcttattg ctgctgtcga
      781 ttgtacgggt catggtgtgc caggtgcttt catgtcaatt atcgggcata actctctcaa
      841 caaggttgta agggaatatg gccttatacg cccgtcagca atactcgacc agctgaatat
      901 tgaggtaagc aagtcaatca tacagagcca tgaaaaaggg attacagatg gcatggacct
      961 ggcactaata gcttacaaca ggaaaaaatt cactcttgag tttgccggtg catataaccc
     1021 gctctatctt gtccgcaatg gggaggttat agtttacaaa ggtgacaggt tcccgattgg
     1081 catggtaaca attgaacaga aaaagaaatt tacgaacgtg aatgttgata tacaaccggg
     1141 tgatatgatc tacatgtgtt ccgatggtta tgctgatcag ttcgggcagg ctgaaaacaa
     1201 gaaattcaag gtgggcaata tcaaaaggct gctttcggaa atatataatc tcccggttaa
     1261 tgaacagaag gcaaggcttg aaaaggaaat aatggactgg aaaggagatt taccccaggt
     1321 ggacgatata ttatttatag gtatgagggt tgtaccttaa tcaatttttg cgaaactacg
     1381 ggggttatca taccatcctt tataatttct tatagaatct atcacttcag acgggtagtt
     1441 tactacattc cagatctccc catgttcttt tctcataaaa tttttccgaa ccatctcctc
     1501 gagaaaattg aggaattgat cgtaaaaatt gagagtgttc aaaatgacta ttggaccttt
     1561 gaatagaccc agttgtttaa gtgtgattgc ttcagcaagt tcttcgagag ttcctatacc
     1621 gccgggaagt gccacaattg catctgccat ttgcatcatc agctgtttgc gttgaggcat
     1681 accttctgta acaatcattt cctttacact gctgtggccc cagccttcat cctgcatgaa
     1741 acggggtata acacctgtta tttttccatt gttgttaagg actgcatctg ccagtcgacc
     1801 cataagtccg atacccccac cgccgaaaac aacctccatt tctgaagcag ccagcattcg
     1861 gccaagctct ccggcagctt cataatacct ctcatctacc ctgttgctcg aagcagcaaa
     1921 aacacaaact ctcatcagtt tttacgatta tctgtagcac attccctgaa ggaggattta
     1981 aagctacaaa agcttttcaa gtttatctgt aaaaattttt tttgccacgg caccgatttg
     2041 acggtcaact tctttgccgt tcttgaagta aaaaactgca gggatgtttc ttacattcat
     2101 ttcttttact actttcggat tattatccac attgcattta gccacaacaa ctttgccttc
     2161 atattctttt gaaatttctt caatgaaagg ggctattctt ctgcatgggc cacaccattc
     2221 agcccagaaa tcaacaataa ctggtttttc tgatttcaga acgatttctt cgaaattgga
     2281 gtctgtaact tctaatacca taattttgtg ttctttaatt gattggactt aagtgagaat
     2341 aacaaatata attaattcac tttaaaatca agtgcaggat ggtcttcaag aaaagtaatg
     2401 aaatcattat taagtcttac tttgattgtt ctggaaaaca ttggcagtga aattttgctt
     2461 tcggggtcaa tgaagaggaa tcttagttcc gtttcaccag gattggcttt tatcatttta
     2521 gtaagctggt taataagttc gttgtccaca tcgggtgcct taaccttaat tgtgacactt
     2581 tttatcagtt catccttcac actcgaaagc agggtaattg attttactct gaagtcgagt
     2641 tcattttcag catattttct tctctgaacc ttacccttta ccagaaggaa atatccgtta
     2701 acaagatatt tactgaattc aacgcaatct ttgtcgaaca aaacgaaccg gaaggagtct
     2761 gtataatcct gcagtgtaaa tgaggcatat ggcttgttgt tcttgctgat gccgttccgt
     2821 gtttcggtaa ccattcctgc aacggccaca tccctttcta tgaactcctg catgttattt
     2881 ctcaattctt caagagtagt gttagtgaaa gtttcaattt ccagtctgaa gtcgtcgagc
     2941 ggatgtgatg aaaggtaaaa gcctataact tctttttccc tgttaagttt ttccagtttt
     3001 ggccattcat cgcaggaggg aggttctggt tttttcatgc tgaacccgtt gtcttcaccg
     3061 aaaagagtgt tcttactgga gttctttatt gttttaatat tgtttccgaa tctgattaag
     3121 tgttcgatga aagttgttcc ttttgcgtca gcagcgaaat attgttcacg tgatatttcc
     3181 ggaaagcagt cgaaagcacc ggcgagagcc attgcttcaa tatttttcct gttgagagta
     3241 ttcagattca cacggcttac gaagtcatag atatctctgt agggaccgtt tttttctctc
     3301 tcttcgatta cctgcataac ggcattttct cctactcctt taatagcacc cagtccgaag
     3361 cgtatgtttc cgtctttgtt tacagtgaat ttcacatcac tttcgttgat atcggggccc
     3421 agaacttcaa tgcccattcg ccgtgcttca tcgatgaaga gggtaatttt cttcaggtcg
     3481 ctgatatttc ggctgagaac agcagccatg tattctgcgg gataatgtgc tttaaggtag
     3541 cctgtctgga atgccaccag ggcatagcag gtggagtgtg acttgttaaa ggcataatgg
     3601 gcgaagcttt cccagtcgct ccatatttta tttattaact gttcgctgta tcccttctta
     3661 aggcatcctt ccatgaagcg cgccttcatc tcttccatga gcgctttttt cttcttaccc
     3721 attgccttgc gcagggagtc ggcttcacct ttcgtgaacc cagccagttc ctgcgaaagc
     3781 agcataacct gctcctggta aacagttatg ccataggtgt cggcaaggta cttttccatc
     3841 tcgggaagaa ggtattcgat cttttcctgc ccgtgtttgc gttttatgta gagtggaata
     3901 ttttccatcg gccccgggcg gtaaagagca ttcatggcaa taagatcctc gaaacggttg
     3961 ggtttcagtt cacggagata ccttttcatt ccgtccgact caaactgaaa tatgcctgtt
     4021 gtatctcccc ggctgaaaag ttcaaaggtc ttcttgtcgt cgagaggaag gttttcaata
     4081 tctatttcaa taccatgcct tttctttata ttctcgatgg catcccttat tatagagagt
     4141 gttttgagcc cgagaaagtc catcttcagc agtcccaccg attcgacatg gttaccttcg
     4201 tactgtgtaa cgaaaagatc tgtttccttt gcagtactta gggggatgta gttatcgagg
     4261 ggttccttac ttattattat tccgcaggca tgtactccgg tctgccttat tgagccttca
     4321 agtgcttcag caaactgcag ggtctgggat atcagtttat tgggggagtc tttttctttt
     4381 gccagttcag gcacttcggc ataagcgtcg gcaagggtta ttccaggtcg ctccggcacc
     4441 atctttgcca gtctgtcagc ttctgacagg ggcaatttct gaacccgcgc cacatctctg
     4501 atagccatct tagctcccat ggtgccaaag gtgatgatat gtgccaccct gtcgtggcca
     4561 tatttattta ccacatacct cagcacctgt tctctgccgt cctcatcaaa gtcaatatca
     4621 atatcgggca atgagattct gtcgggattg aggaaccgct cgaacagaag tccatacttg
     4681 atgggatcta tgtcggttat acgcaggcag taagctactg ctgagccagc tgcagaacct
     4741 ctgcccggcc ccaccgccac acccatttcc ctggcagcct tcagaaaatc atgtacaata
     4801 agaaaatatg cagggaagcc catcctgata atcgtgtcga gctcaaactc aagcctctct
     4861 ttctgctcct ctgtcaatac tccccatctt tcttttgcac cttcataggt aagatacctg
     4921 agatactcac tctccgatga aaactccggt ggaatcttga attcaggcat gataggatca
     4981 tgatcgagct cgtaaagctc aatctttgat attatctctg ccacattttc aattgcttca
     5041 ggaatatcac tgaagatctc cctcatttct tcctcacttt tcagatactc ctgccttgag
     5101 tatctcatcc tctccggatc atcaagatcc tttgcagtat ttatgcatat taacctgtcg
     5161 tgtgcatcag catcttcctt gttagcaaaa tgcacatcat tggtggcaat aatttttact
     5221 ccatactttt ccgacaacct ccggaaggct tcaataacct tttgctgttt cggaaaagtt
     5281 tcaatgtttg cgtcaggctc ggtagtttcg tgacgttgta tttcaagaaa gaagtcttct
     5341 ccgaatatgt caatataact tttcagcgcc ttttcagcac cttcaatgtt tccattgagt
     5401 atctcaccgg gtatttctcc tgcaatgcat gcagaggttg cgataagacc atctctgtgt
     5461 ttttcgaggg cttttttatc aatccttgga gtataataaa aaccttttgt ccaggcaagt
     5521 gaaactagtt ttatcagatt gcggtaaccg gtaagatttt ttgccagcag tatcagatga
     5581 tcacccgatc tgtcttcact gccggttttt tcttccatgc ttcctttagc aatataaatc
     5641 tcacatccaa gcaccggctt tattcctttg cttacggcat aattatgaaa ctccttgaca
     5701 ccaaacatgt taccatgatc agttattgcc agagcatcca ttccgagtga tttagccctg
     5761 tcaacaagtg ccggtataga agaggcacca tcgagaatgg aatactgcgt atgtacgtgt
     5821 aaatgtgaaa ataaagccat tcagaagaaa atgttacata attcagccgg ctttacaaaa
     5881 ataggtaaaa aatcaggtaa ctttgtttga acctttgcat atttaatttt actttacaat
     5941 ctggcatatc ataaaa
//
LOCUS       DAOSNB010000159         5910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_13624_length_5910_cov_4.329804, whole genome shotgun sequence.
ACCESSION   DAOSNB010000159 DAOSNB010000000
VERSION     DAOSNB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13624_length_5910_cov_4.329804"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PLG42_07225"
     CDS             complement(<1..1362)
                     /locus_tag="PLG42_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOU31038.1"
                     /translation="MNIPSRYDPRKTEDRWYSYWMQHGFFRSVPDDREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDVLIRRARMLGKNACWVPGTDHASIATEAKVVAKLKSEGIEK
                     RDLTREEFLKHAWEWTHKHGGIILEQLKKLGASCDWERTLFTMDEKRYRSVIKAFVQL
                     YNKGLIYRGVRMVNWDPEARTAVSDEEVIYTEEQSKLYYVKYRIAGSDDFITVATTRP
                     ETILGDTAVCANPRDKRYKHLAGKRVIVPMAEREVPFIFDDYVDMEFGTGCLKITPAH
                     DINDYEIGLRHNLPVIDIFNDDGTLSEKAGKFIGQDRFRARELAEEELRRTGHLVKVE
                     KYTTKIGRSERTNAVIEPKLSVQWFLKMKEISVPALNAVLTGEVMLHPSKFKNLYRHW
                     MENVRDWCISRQLWWGHRIPAWYYNDNEFVVAETEEEALELARSKTGNRHLTLKDLRQ
                     DEDV"
     gene            1571..2770
                     /locus_tag="PLG42_07230"
     CDS             1571..2770
                     /locus_tag="PLG42_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOU31039.1"
                     /translation="MPSISQKGREMPPSPIRKLVPYAEQAKRKGIKVYHLNIGQPDIP
                     TPDVALNAIRNLNLRILEYTHSAGNESYRRKLAGYYNSIGIHIDYTNILVTTGGSEAV
                     LFALMTCVNPGEEVITPEPFYANYNGFATAAGIKIVPVTSYIKDDFALPPMSEIEKKI
                     TAKTKGIIICNPNNPTGYLYTKEELYILRDIIKKHDLFLFCDEVYREFCYDGAEHFSS
                     MKLEGIEQNVVMLDSVSKRYSECGVRIGAIVTRNKEVLDTALKFAQARLCPPGLGQVV
                     AEASLDTPPEYFAEVKHEYTNRRNFLVEALNKIPGVYCPKPKGAFYTVVKLPVDDAER
                     FAQWLLEEFEYKNQTVMVAPASGFYSTPGMGKNEVRIAYVLNTADLKNAVEVLAEALK
                     VYPGRES"
     gene            complement(2773..3852)
                     /locus_tag="PLG42_07235"
     CDS             complement(2773..3852)
                     /locus_tag="PLG42_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOU31040.1"
                     /translation="MRPEVYMKFLKGKKVAVVANQSSLAGDKHVVDKLIAEGIDIRLI
                     FAPEHGFRELADAGSKIQNGKDPQTGIPIVSLYGKIYKPSPEHLSGIDVVVYDIQDVG
                     ARFYTYISTMHYVMESCAENGVTFVVFDRPNPNGFYVDGNLPDTAYRSFVCMHPVPVV
                     YGMTPGEYAMMINGEGWLAGGIQCDLKIVKCKNYTHNTYYELPVRPSPNLPNQTAVYL
                     YPSLCFFEGTVISCGRGTDFPFQAFGHPLLPDRGFSFTPHSVPGATNPPLLGQKCYGT
                     DLRNAIKEGLVPSKELNLNWLLTAYREFPDKGKFFNNYFDVLAGGPELREQIIKGMNA
                     GEIRQSWREDIEKFMKVRRKYLLYK"
     gene            4107..5351
                     /locus_tag="PLG42_07240"
     CDS             4107..5351
                     /locus_tag="PLG42_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU31041.1"
                     /translation="MNLPYFIAQRLIKGRREGASFSKPINVIAIAAIAIGIAVMILAV
                     AILTGFKQQITDKVVGFGSHIQIVNFDSNISYETAPVSTNLSFIPKIKDMPGIKHVEV
                     FATKAGIIKTDEDIQGVVLKGIGSDFDWSYFKTCIVDGSVFIVTDTATTNSVIISKKI
                     ASMLKLKTGDSFAMHFVQDPPRMRKFTISGIYETSLEEFDKIYIFCDIKHIQRLNGWK
                     NDEVSGFEIFIDDFNRLDIMTQAVRDVIGYRISENEPKVKVTNIRMKYPQIFDWLNFQ
                     DTNVIVIIVLMLVVSGFNMISGLIILILEKTNMIGILKALGSEDGTIMKVFLYQALYL
                     IVKGLFWGNIIGIGLASLQLWTGLISLDPSSYYLKTVPINLEAVHILLLNLGTMAIIV
                     LVLLIPAQLVSRITPVKAIRFD"
     gene            complement(5410..>5910)
                     /locus_tag="PLG42_07245"
     CDS             complement(5410..>5910)
                     /locus_tag="PLG42_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOU31042.1"
                     /translation="RYSETINWDWRLLASLIYQESRFKSNAVSWAGAHGLMQVMPETG
                     QRFGVDVTSSPENNLRGGVLYLQHIQKYFEDKIPDEGERIKFVLAAYNAGIGNVLDAM
                     RLAEKYGRNPLLWDDNVAYFLLKKSDPEYYNDPVVKHGYCRGSEPVNFVAEILARYSE
                     YRNFIP"
BASE COUNT         1746 a         1282 c         1105 g         1777 t
ORIGIN      
        1 tacatcctca tcttgccgca aatcttttaa tgtaaggtgc ctgtttcctg tcttactgcg
       61 ggcaagctca agagcctcct cttcagtttc tgccacaaca aattcattat cattgtaata
      121 ccatgcagga atgcggtgac cccaccagag ctggcgactt atgcaccagt cgcgaacatt
      181 ctccatccag tgacggtaaa gatttttgaa ctttgaagga tgaagcatca cttcacccgt
      241 cagtacagca ttaagtgcag gaactgaaat ctccttcatt ttaagaaacc attgaaccga
      301 aagctttggc tcaattaccg catttgttct ttccgacctg cctatcttgg ttgtatattt
      361 ttccactttt acaagatgtc ccgttctcct aagttcttcc tctgccagct cccttgccct
      421 gaaacgatcc tgccctataa actttcctgc tttttcactc agagtgccgt catcattgaa
      481 aatatcaatc acaggcaggt tatgccgcaa tccaatctca tagtcattga tgtcatgtgc
      541 cggggttatc ttaagacagc cggttccgaa ttccatgtca acataatcgt caaaaataaa
      601 tggcacctcc ctttctgcca ttggaacaat aaccctcttg cctgcaagat gtttatacct
      661 tttatcgcga ggattggcgc atactgctgt gtctcccagt attgtttcgg gtcttgtggt
      721 ggcaacggta atgaagtcat ccgaacctgc tatacggtat tttacataat aaagcttcga
      781 ttgctcttct gtataaataa cttcttcatc ggaaacggca gttctggctt cggggtccca
      841 gttgaccatt cttacacctc tgtatatcag acccttgtta tatagttgaa caaacgcttt
      901 tattaccgaa cggtatctct tttcgtccat tgtaaaaaga gttctttccc aatcgcatga
      961 ggcacccagc ttcttaagct gctcgagaat tattcctcca tgtttatgtg tccattccca
     1021 tgcatgttta agaaactctt cccttgtgag atcccttttt tcaattcctt cactctttaa
     1081 ttttgccacc acttttgctt cagtggctat cgaagcatgg tcggtacccg gaacccagca
     1141 tgcattcttt ccgagcatcc ttgcccttcg gatcagaaca tcctgaatag tattattgag
     1201 catatgtccc atatgcaata cccctgtaac attgggcggg ggaatcacaa tggtataagg
     1261 ctccctatca tccggtacac ttctgaaaaa tccatgctgc atccagtaac tgtaccacct
     1321 gtcttcagtc ttgcgtggat cgtatctcga aggaatattc atcatctgaa aaatgatatt
     1381 tgcagtaatt acaaagctgc aaaattataa aaaaacagtg atattacttg acagaaagca
     1441 tttcgaccta attaaatatg caaactgtta taatctaagt cttaatgata tctatcaatt
     1501 tatgttttaa ataatctttt acttatttca aattctttac atttgcacac tgactataat
     1561 taaatttatt atgccatcaa tatctcagaa aggcagggaa atgcctccat cacctataag
     1621 gaagcttgta ccctatgccg agcaggcaaa acggaaaggg ataaaagttt atcacctcaa
     1681 tatcggacaa ccggatattc ccactcccga tgttgctctt aatgccatca ggaatctgaa
     1741 cctcagaatt ctggaatata cccattcagc agggaatgaa tcgtaccgcc gaaaacttgc
     1801 aggatattac aactcaatag gcatacatat tgattatact aatattttag ttacaacagg
     1861 aggttctgag gctgtattat ttgcgctcat gacatgtgtt aacccgggag aagaagttat
     1921 tacccccgag ccattttatg ccaattataa tggattcgca actgctgcag gaattaagat
     1981 cgtgcctgtt acttcataca taaaagacga ctttgcactg ccaccaatga gcgagattga
     2041 gaaaaaaatc actgcgaaaa caaaaggtat aataatatgc aatccgaata atccaactgg
     2101 atatctctat actaaagaag aactttacat tttgcgggat ataattaaaa aacatgatct
     2161 ttttcttttc tgcgatgaag tttacaggga attttgctac gacggggctg aacatttttc
     2221 gtcgatgaaa cttgaaggca ttgagcaaaa tgttgttatg ctcgactcag tttcaaagcg
     2281 ttacagtgag tgcggtgtaa gaatcggggc aatagttaca agaaacaaag aagtacttga
     2341 tactgcactt aaatttgctc aggcaagact ttgtccgcca ggtcttgggc aggtggttgc
     2401 agaagcatct ctcgacaccc cgccggaata ctttgccgaa gttaaacacg agtatacgaa
     2461 cagaagaaat ttccttgtgg aagctctaaa taaaataccc ggcgtatatt gtcccaaacc
     2521 aaaaggagct ttctacactg ttgtaaaact gcctgttgat gatgccgaac gttttgcaca
     2581 atggcttctt gaagagtttg aatataaaaa ccagacagtt atggttgctc ctgcctcggg
     2641 cttttattca actccgggca tgggaaaaaa tgaagtcaga attgcttatg tgctgaatac
     2701 cgccgattta aaaaatgctg ttgaagtact tgccgaagca ttgaaagttt atccgggcag
     2761 ggaaagctaa aattatttat agagaagata ttttctcctt actttcataa atttctcaat
     2821 gtcttctctc cacgattgcc gtatttcacc ggcattcata ccttttataa tctgttctct
     2881 tagctcagga ccaccggcaa gtacatcgaa ataattgtta aagaattttc ccttatctgg
     2941 aaattcccgg tatgctgtca acaaccaatt gagattgagt tccttagatg gtacaaggcc
     3001 ttcttttatt gcatttcgta agtctgttcc gtaacatttt tgccctaaca aagggggatt
     3061 tgtagctccc ggaacactgt gcggggtgaa actgaagcct ctgtcgggca gtaaaggatg
     3121 tccgaatgcc tgaaaaggga aatctgttcc tctaccgcat gaaatcacag ttccttcaaa
     3181 aaaacagagt gaagggtaaa gatatacagc tgtttgattg ggcaaattag gtgaaggcct
     3241 tacaggaagt tcataatagg tgttgtgtgt atagttttta cattttacaa ttttcaggtc
     3301 gcattgtatg cctcctgcta accatccctc accgttaatc atcatggcat attcacccgg
     3361 ggtcatacca taaacaacag gcaccggatg catgcagaca aacgacctgt aagcagtatc
     3421 aggcagattt ccatcaacat aaaaaccatt cggatttggc ctgtcaaaaa ccacaaatgt
     3481 cacaccattt tcggcacagc tttccataac ataatgcatt gttgaaatat aggtatagaa
     3541 acgtgccccc acatcctgaa tgtcgtaaac aaccacatct attccgctaa ggtgctccgg
     3601 agatggttta taaatcttac cataaaggct cacaattggt attccggttt gtggatcttt
     3661 tccattctgt atcttgctac ctgcatcagc tagctctctg aaaccatgtt caggagcaaa
     3721 aatgagcctg atgtcgatcc cttcagcaat aagcttgtcc acaacatgct tatctcccgc
     3781 caacgatgac tgattagcca ctaccgcaac cttcttccct ttcagaaatt tcatgtacac
     3841 ctctggcctc atatctcctg ttatcacctc acgactgttt ttcgcgcagg caatcgatga
     3901 aaggcagaga aatattaaaa ctaatctctt aactatcata aagaaattat tttttaaata
     3961 tcttatcatt tccatttttc cggccaggtc actaccatgt tgaaagagtg tctcactaca
     4021 accaatggca tatgaagata agtgtttttt aaaagcaatc atcttgttga tgcaaattgt
     4081 taaatttgaa gtcttatctg ggaaaaatga atttgcctta ttttattgca caaaggctca
     4141 tcaaaggcag acgggaagga gcatcgttct caaagcccat caatgtaatt gccattgctg
     4201 caatagccat aggtattgct gtgatgattc ttgcagtagc catcctgaca ggtttcaagc
     4261 agcagattac tgataaggtt gtaggatttg gttcacatat ccagatagtc aattttgatt
     4321 ccaatatttc gtacgaaacg gcaccggtta gcacaaacct ctcttttata ccaaaaataa
     4381 aagacatgcc gggtataaag catgttgaag tatttgccac caaagccggt attataaaaa
     4441 ccgatgaaga tatacaggga gtggttctga aaggcatcgg atccgatttc gactggagtt
     4501 atttcaaaac atgtattgtc gacggttcag tattcatcgt taccgataca gccacaacaa
     4561 acagcgtaat tatttcaaaa aaaattgctt cgatgctgaa gctgaaaacc ggcgattcct
     4621 tcgccatgca ttttgtacag gatccaccga gaatgaggaa attcacaatt tcaggaatat
     4681 acgaaaccag tcttgaagaa ttcgataaaa tctacatctt ctgcgacatt aaacatatac
     4741 agaggctcaa cggatggaaa aatgacgagg tcagcggatt cgaaatcttt attgatgatt
     4801 tcaacaggct cgatataatg acccaggcgg taagagatgt aataggttac agaatcagtg
     4861 agaatgaacc caaagtcaaa gttaccaata tcaggatgaa atacccacag attttcgact
     4921 ggctcaactt tcaggataca aatgtgatag ttataatcgt tctgatgctt gtagtatcgg
     4981 gcttcaacat gatttcgggt cttattatcc ttatacttga aaaaacaaat atgataggta
     5041 ttcttaaagc actcgggtcg gaagatggca caataatgaa agtatttctt tatcaggctt
     5101 tatatcttat cgttaagggt cttttctggg ggaatattat tggaattggg ctggcatctc
     5161 ttcagctgtg gacaggttta atatctctcg atccctcttc atactactta aaaacagttc
     5221 caattaacct ggaagctgtt catattcttc ttttgaatct cggtacaatg gcaataattg
     5281 tacttgtcct gcttattccc gcacaacttg taagtagaat aacccctgtc aaggcaatta
     5341 gatttgatta gctgaactgg aaatatttca gcaggttttc cgataaaata acagaactag
     5401 ctctggaaat cagggaatga agttacggta ttcagagtat cttgcaagta tttcagctac
     5461 aaaattgaca ggctcagagc ctctgcaata accgtgtttt accaccgggt cgttataata
     5521 ttccgggtct gattttttta acaagaaata ggcgacattg tcgtcccaca gtaatgggtt
     5581 tctaccatat ttttctgcaa gcctcatagc atcaagaaca ttacctattc cggcattata
     5641 ggcagcaagt acgaatttaa ttctttcccc ttcatcaggt attttatctt caaaatattt
     5701 ctgaatgtgt tgcagataaa gcaccccgcc tcttaaatta ttctcgggtg atgaagttac
     5761 atctacacca aatctttgac cggtttcagg cataacctgc ataagtccgt gagcaccagc
     5821 ccacgagact gcatttgatt taaatctcga ttcctgatat attaacgaag caagaagtct
     5881 ccagtcccag tttattgtct cgctatacct
//
LOCUS       DAOSNB010000160         5746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14143_length_5746_cov_3.274117, whole genome shotgun sequence.
ACCESSION   DAOSNB010000160 DAOSNB010000000
VERSION     DAOSNB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14143_length_5746_cov_3.274117"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLG42_07250"
     CDS             complement(<1..914)
                     /locus_tag="PLG42_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HOU31043.1"
                     /translation="MREKYKLRIGLFGIGLDTYWPQFKGLKERLEAYLEEVCGKLKNF
                     NAYIVSLGLIDNPVSALEAGHKFRREDVDIIFLYVTTYALSSTVLPVVRRAKVPVIIL
                     NLAPEKAIDYQIFNNLNNRTAMTGEWLANVSACPVPEIANVFMRAGIRFHQITGVLHG
                     DEEVWKEVSEWVEAANVANILAHNRLGLMGHYYGGMLDVYTDLTLQLTSFGGHIEMLE
                     VDELSCLRRKISEQEIADRLELFYMEFDIDDGCAASDLRQAAITSVALEKMIEKHDLG
                     SLAYFYSGTGIQENEDSISTIILANSLLT"
     gene            1446..2027
                     /locus_tag="PLG42_07255"
     CDS             1446..2027
                     /locus_tag="PLG42_07255"
                     /inference="COORDINATES: protein motif:HMM:NF025431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HOU31044.1"
                     /translation="MISETLNFLATELNKFLNQKMGPTTDPRLLVGNIIKAFEGESGK
                     DPVNKIFLSLINIEEDKVAKISENYTKTETAVIYKNPPLFVNLYILIAANLGNYSDSL
                     KAINFIMQFFQSQNCFTPVTHPGLDSRISQISADLFSLNFEQINHIWSTLGGKYLPSV
                     MYKLRQLKIEDEEAAAAEGRLIETIITETRAGR"
     gene            2027..3223
                     /locus_tag="PLG42_07260"
     CDS             2027..3223
                     /locus_tag="PLG42_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31045.1"
                     /translation="MNVRYGILFSVAILNDYFSSGKWKEISFLPLPETEKLIGSGGFR
                     IIKVHNQLLVVTRVDKDMKPLRLPDFFTGFSFFLQPQTPNYINFSNLPFRESRIKSLH
                     FHNLHNNVSGGVNYLSSAIENYSPVITYKPGEFCLGSDRSVYESIKINNNIQAPDDTD
                     TGSKEYWVKHGDTQLVSERDTIAVDADESFEIKKTGPVFNFKAAVKRTVHNVNVYACN
                     FSSGTYSNEVYHDIVRFDSPHDMVQVDMRSLSSGLYRIRVENEVNFVYMVSSGIDSDS
                     PFFLEIFNLPPSDPRSLVMPDGTLKNIKYTIHFASRRVFWRYRTRTTVIDNIKDSAGE
                     FKFKPDGPRFFISETPIPFSDTPRKTFSADSGNLVIASPLPNPMADRLLNKRDEIFTT
                     EISVNS"
     gene            3244..5178
                     /locus_tag="PLG42_07265"
     CDS             3244..5178
                     /locus_tag="PLG42_07265"
                     /inference="COORDINATES: protein motif:HMM:NF037565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HOU31046.1"
                     /translation="MATVYKTPGVFIEEISKFPPSVAQVETAIPAFIGYTEKAEDLAP
                     DDLIKKPRRIGSLVEFSYYYGNGPALKVSEVAIDVSNNFKSAKITSDYYMYDSLRLFF
                     ANGGGDCFIVSIGKYPAAVTAGDFTAGIDTLLKEDDPTILLFPDAVTLGTSDLAAVQT
                     HALNHCGDESRLDRVTVFDLKEDDPKGATFRSNIGTSFLSYGIAYTPWLKTNLPKKVT
                     FPDIKDVIKKNGASVTLKGLTGDPAIQTQIDELEKAYADKTKIISGTDTLASPSANLR
                     DKYNSVVATYNAGKTTGNLQAVLTYVLSIIWKTDDYLKTGNAAELTNTALKNSVTANI
                     STFSTEAKKIIGLDKDLKAKFGAGYTTQAKAADFTDGIWGAGFVAGTAASGLLTAATD
                     EENLELALNALNNIFDTLSRAYLSLIVNAAASNARDADTALSLAFPLYKNILKGVSTY
                     SASIPPSGAIAGIYAMVDRTRGVWKAPANVSLANVIEPTVTFTKSELDALNVDVNAGK
                     SINAIRTFFGKGTLVYGARTLAGNDNEWRYVPVRRLFIMVEESVKKATEQFVFEPNDA
                     NTWVKVQAMIENFLHIIWRQGALQGAKPEHAFYVAVGLGKTMNPIDILEGRMIIEIGM
                     AAVRPAEFIIIRFSHKMAES"
     gene            5229..5657
                     /locus_tag="PLG42_07270"
     CDS             5229..5657
                     /locus_tag="PLG42_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOU31047.1"
                     /translation="MANYPLPKFHFMVEWGGNKIGFTEVTGLDMQVEAIEYREGSSKT
                     YSKVKMPGLHKYSNITLKRGTFEGDTDFYTWINTINLNTVERRDIVIKLLNEKHEPVV
                     TWRAVNAFPLKVQASDLKADGNEVAIESIEIAHEGLSIVK"
BASE COUNT         1699 a         1196 c         1257 g         1594 t
ORIGIN      
        1 gtgagcaatg aattggcaag tatgattgta cttattgaat cttcattttc ctgtattccc
       61 gtgccgctgt agaaatatgc cagtgaaccc agatcatgtt tttcaatcat tttttccagg
      121 gcaactgatg ttattgcagc ctgtctgagg tcagatgcgg cacaaccgtc atcaatatcg
      181 aattccatgt aaaacagttc aagacggtcg gcgatttcct gttcggaaat ttttcttctg
      241 agacatgaga gttcatcgac ttcaagcatt tcaatgtgac cgccgaagga ggtcagctgg
      301 agggtaaggt ccgtgtaaac atcgagcatt ccgccataat aatgacccat taaaccaagc
      361 cggttatgtg ccagtatatt ggcaacattt gctgcctcaa cccattcact aacctctttc
      421 catacctcct cgtcaccatg aagaacgccg gttatctggt gaaatctgat acctgctctc
      481 atgaagacat tcgctatttc gggaaccgga caggcagaga catttgcaag ccattccccg
      541 gtcattgcgg tccggttatt caaattatta aagatttgat aatctatggc tttctccgga
      601 gcaagattaa gaataatcac aggtaccttg gctctccgga ctaccgggag taccgtggag
      661 gaaagagcat aggtggtaac atataagaat attatatcaa catcctctct tctgaattta
      721 tgtcctgcct ccagggcgct gaccggatta tcgatcagtc cgagactgac aatataagca
      781 ttgaagtttt tcagttttcc gcagacttct tccagatatg cttcgaggcg ttccttcaat
      841 cctttgaact ggggccagta ggtatcaagt ccgatcccga aaaggccgat tcttagtttg
      901 tatttctctc tcatggttaa ttacagggtt ttaatgttca gtttttcacg gagtttcaaa
      961 gtttaggggt tcaggagtta cagagtattg ctctcactcc tcatggcaat ttacaaatta
     1021 ttggcgttag tgcggtaaaa accaattttc tgattatttt atcatttatg atcagggtgt
     1081 tacaaataaa tgcagaatta attttgcaga gcgtcacttg tcgggaaata ctgtcagctg
     1141 cgttaaggaa ttatagtccg cccggagaat ttttatttga cttggggttt cgtatttcgt
     1201 aactttaaca atagaataaa aaaccttctt ccaaagtacc agatttcaga aattccaggg
     1261 ataagcgttg aattaaacct aatacagttc gccatgaaat tgaaaatctg ctacttcggt
     1321 tgcctgaaca gtctgttata gcagaacctg tttttttaaa aaacaactga acttttttta
     1381 cccggtaaat gttattaata aggcgagttt tctgaattgg atctggtttg ttaaatactt
     1441 cccgtatgat tagtgaaacg ctgaacttcc ttgccaccga actcaacaag ttcctgaatc
     1501 agaaaatggg tcccacaacc gatcccaggc tcctggttgg taatataata aaagcatttg
     1561 aaggagagtc aggtaaagat cctgttaata agatcttcct ttcattgatc aatattgagg
     1621 aagacaaagt tgcaaagatc agcgaaaatt acacaaagac agaaacggcg gttatttaca
     1681 agaacccgcc attgtttgtc aatctttata tccttatagc tgccaatctg ggaaattaca
     1741 gtgacagtct gaaagctata aatttcatta tgcagttttt tcagtcacaa aactgtttta
     1801 cgccggttac acaccccggg ctcgatagca ggatatcaca gataagcgcc gatctgttct
     1861 cacttaattt tgaacagatc aaccatatct ggagcacact gggtggcaag taccttcctt
     1921 ccgtaatgta taagctgaga caactgaaga tagaggatga agaagctgct gccgcagaag
     1981 gcagactgat tgaaacgata ataactgaaa caagggcagg acgctgatga atgtaagata
     2041 cggaatatta ttttcggtcg caattcttaa cgattatttc agttcgggta aatggaagga
     2101 gatctctttt ttaccgctgc cggaaacgga aaaacttatc ggttccggag gcttcaggat
     2161 aattaaggtt cataatcaat tgcttgtggt tacaagagtg gataaagata tgaaaccact
     2221 caggttacct gattttttta ccggattttc attttttctt cagccgcaga ctccgaatta
     2281 cataaacttc tcaaatctcc cgttccggga atcccgtata aagagcctgc attttcacaa
     2341 tctgcataat aatgtctcag gcggtgtgaa ctacctttca tccgcaatag agaactactc
     2401 cccggttatt acttataaac cgggagaatt ttgcctgggt tccgacagga gtgtatatga
     2461 atcaattaaa ataaataata acatccaggc tcctgatgat accgataccg gttcaaagga
     2521 atactgggtt aaacatggtg atactcagct tgtaagtgaa agagatacta ttgctgtcga
     2581 tgcagatgaa agttttgaga tcaaaaaaac cggaccggta tttaatttta aggctgcagt
     2641 caaaaggaca gttcataatg tgaatgtata tgcttgtaat ttttcctcag gtacttacag
     2701 caatgaggtt taccacgata ttgtccgctt cgacagcccc catgacatgg tgcaggtaga
     2761 tatgcgctcc ctgtcctcag gattatacag gatcagggtg gaaaatgaag tcaattttgt
     2821 ttatatggtt tcctcgggca ttgattcgga ttcacctttt tttcttgaga tatttaatct
     2881 gcctccatct gatccgcggt cacttgtcat gcccgacggt acattgaaga acataaaata
     2941 taccattcat tttgccagca ggagggtctt ctggaggtac aggaccagaa cgacagttat
     3001 agataatatt aaggactctg caggtgagtt taaattcaaa cctgacggac cgagattttt
     3061 tatttcggag acgcccatcc ctttcagcga tactccaagg aagaccttca gcgctgattc
     3121 aggtaacctg gttatagcct cgccattgcc taatcctatg gccgacaggc ttttaaataa
     3181 gcgtgatgaa attttcacca cagaaatatc cgttaattcc taaaaaattt aaatccgaaa
     3241 tttatggcaa ctgtttacaa aacacctggt gtttttatag aagagatatc gaagtttcct
     3301 ccttcggtag ctcaggtaga aactgcaatt cctgccttta tcggttatac ggaaaaggca
     3361 gaggatcttg caccggatga tctcatcaaa aaaccgagac gcatcggatc gctggttgaa
     3421 ttttcatatt attatgggaa cggccccgct ttaaaggtga gcgaggttgc catagatgta
     3481 agcaataatt tcaaatcagc aaagatcact tccgattatt atatgtatga cagtctgagg
     3541 ctgttctttg caaacggggg cggcgattgt ttcatagtaa gtatagggaa atacccggca
     3601 gcagttacgg caggcgattt tacagcgggc atagatacac ttttaaagga ggatgacccg
     3661 acaattctgc tgtttcctga tgctgtgaca cttggaactt cagatctggc tgccgttcag
     3721 acacatgctc ttaaccattg cggcgacgaa agcaggctcg accgtgtcac agtatttgat
     3781 ctgaaagagg atgacccgaa aggagcaacc ttccggtcaa acattggtac cagcttcctt
     3841 agctatggga tagcctatac tccctggtta aagaccaatc tgcccaaaaa ggtcactttc
     3901 cccgacataa aggatgtgat aaagaaaaac ggtgcatctg ttacccttaa aggattgaca
     3961 ggtgatccgg ctattcagac ccagatagat gaactggaga aggcctatgc cgataaaaca
     4021 aaaatcatct cgggaactga tactcttgca agtccgtcag ccaatctcag ggataaatac
     4081 aattcagtgg ttgccactta taatgcaggc aagaccaccg gaaatctgca ggcagtactg
     4141 acttatgtgc tgtcgattat ttggaaaaca gatgattatc ttaaaacagg caatgcagca
     4201 gagctgacaa atacagctct taaaaattca gttactgcta atatatcaac gttttcaact
     4261 gaagcaaaga aaataatcgg cctcgacaag gaccttaagg ctaaatttgg ggcaggttat
     4321 accacgcagg cgaaagctgc cgattttaca gatggaatat ggggagcagg ctttgttgca
     4381 ggaaccgcag cttctggctt gctgaccgct gcaacggatg aagaaaacct ggagttggcc
     4441 ctgaatgccc tgaataacat tttcgatacg ctgagcaggg catacctctc gctcatcgtc
     4501 aatgcagcag cttccaatgc cagagatgcc gataccgctt tatcacttgc attcccgctg
     4561 tataaaaata tccttaaggg ggtaagcact tactcagcct caatacctcc gagcggagcc
     4621 attgccggaa tatatgcaat ggtcgaccgt acgcgcgggg tgtggaaagc tcctgccaat
     4681 gtgagcctgg caaatgtgat tgagcctacg gtaactttta ccaaatcgga gcttgatgca
     4741 ctgaatgttg atgtgaatgc cggcaaatca ataaatgcca taaggacttt cttcggcaag
     4801 ggaacacttg tttatggtgc ccgtacactt gcaggtaatg ataacgaatg gcgttatgtg
     4861 ccggtccgca gattgtttat catggtcgag gaatcggtca aaaaggcaac cgaacaattt
     4921 gtattcgagc ctaatgatgc caacacatgg gtgaaagtac aggctatgat cgaaaacttt
     4981 cttcatataa tatggcgtca gggagcctta cagggcgcaa aacccgaaca tgctttttat
     5041 gtggcagtcg gattgggtaa gacaatgaat ccgattgata tcctggaagg aaggatgata
     5101 atagaaatag gcatggccgc tgttcgtccg gctgaattta ttatcatacg tttctcccat
     5161 aaaatggccg agtcctagtt atttgtatat cagagtaaaa taattttcaa atattaaatt
     5221 ataaaataat ggcaaattat cctcttccga aattccattt catggtagaa tggggtggca
     5281 acaagatcgg atttaccgaa gtaacagggc tcgacatgca ggttgaagcg atagaatacc
     5341 gtgaaggttc aagcaagaca tattccaagg tcaagatgcc cggcttacac aagtacagta
     5401 acattactct taagcggggc acttttgaag gagatactga tttctatacc tggatcaaca
     5461 ccataaatct caatactgtg gaacgcaggg atatagtgat caaactgctt aatgaaaaac
     5521 acgaaccggt agttacctgg agggcagtca atgccttccc gctgaaagtt caggccagtg
     5581 accttaaagc cgatggcaac gaggtggcaa tcgaaagcat agaaatcgct cacgaaggcc
     5641 tcagtatagt gaaatagtgg ctttcgcata aagatcctac aggtaaaaac tgcaaactat
     5701 ggctgacagc aaattacctt cggcaggcgg ttattatcct cctctg
//
LOCUS       DAOSNB010000161         5719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14233_length_5719_cov_4.429555, whole genome shotgun sequence.
ACCESSION   DAOSNB010000161 DAOSNB010000000
VERSION     DAOSNB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14233_length_5719_cov_4.429555"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..1633)
                     /locus_tag="PLG42_07275"
     CDS             complement(314..1633)
                     /locus_tag="PLG42_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOU31048.1"
                     /translation="MKQFKILISLIILSASIMNLYCQDWPQYRGPGRNGISNEKGILR
                     SWPKTGPEVLWSVDVGIGFGGPVVKDGKVYLLDRDDKVGDKLRCFDLASGKELWSFSY
                     DAPGSVQFPGSRSVPAIDGNRIYTCGPYGHLYCIDVNTNKPLWNKNIWTDFGGGQIPR
                     WAITQCPLVHGDLLIIASQAPQAGVVAYDKLTGNLKWKTPSLGPAGYVSPVIVKIGNE
                     NHVVMISASAGRGESATPSVVAGIDPRSGKVLWEYKGWSCRIPVPCALDAGEGRILIT
                     GAYEAGSAMIKVQKKSDGSYEVTELYKNPDFGAHTQAPILYNGYFYAQYSTNERKDGL
                     VCMSMNGQVKWKTGREPLFDKGGMILADGLLLCTDGMTKLYLIEPDSTKFKPVAETVI
                     LNEGIPATNEDTAARYGSAQNWAALALAGGKLLVRDQTKLYCIKVSR"
     gene            complement(1853..2641)
                     /locus_tag="PLG42_07280"
     CDS             complement(1853..2641)
                     /locus_tag="PLG42_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31049.1"
                     /translation="MKGKISYLMFALLAMSSYIITFAQKTDSTLIAGHFGGAVSLTNN
                     GISLIPNLTLGKPAVIFDMSVGKRKLSFEPQLRFACEGKPWTFIFWWRYKLLENERFR
                     IIIGTHPALAFKTESFTADGNPKKLTIVRRYLAGEFYPSYNLTKSINTGIYYLYAFCL
                     ENDAIKNTNFLALRVNFSHIGLSGNYFLRFNPQIYFLKMDEMKGYYFTSAITLVRKNF
                     PLTVSSMMSLPFNTFIHADKDFVWNVSVVYSFNKEYIERKILSM"
     gene            2960..4000
                     /locus_tag="PLG42_07285"
     CDS             2960..4000
                     /locus_tag="PLG42_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOU31050.1"
                     /translation="MKRGFRSHSLIKLLVVGMISAVFVLYPNIAFFSFERDYLGEAKY
                     AAYLFFFIFRFVYFSLLIWVLLVYNLRKLKTPFLTKRLIYTFFITAAGYLVYIVFSIL
                     LSPKPEWYSGLLLFQFFVMFIFCGLIGHVFHLYEEQHRKEQEIERLKHENLQSRYIAL
                     ANQINPHFFFNSLNGLVSVIRKKNEENAIEYVNKLSDVFRYILQSDRKGLVLLEDELE
                     FVRAFQYMMEVRFASKLVFSINVSDEMKALKLPVLSLLPLIENVVLHNTIDSEHHMEV
                     DIYVNSERELVVSNPVYPKLSPPETNGTGLKNLENRFILLMNKHIRVEKDNIRFTVYL
                     PLKSKNDEDTDS"
     gene            3981..4739
                     /locus_tag="PLG42_07290"
     CDS             3981..4739
                     /locus_tag="PLG42_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOU31051.1"
                     /translation="MRILIVEDETAAYENLLSVIKKIDPEIEIAGCAESVRQTIRWLR
                     ANPIPDLILMDIHLSDGSAFSIFDSINVESPVIFITAYDDYAIEAFKVNSIDYILKPI
                     KEEDLRRALEKFKKLTHHDILKYLAQMTQMVSLPVYKDKLLIPEKDKLIPVDINEISC
                     FYSTEKNTRVFLKNGKSYQWNRSLEQIEEGLNPADFIRANKQYIISRSSVKSITVWFD
                     NRLLITLDTETPERIYVSKNRAAEFKAWMMHGKK"
     gene            4876..>5719
                     /locus_tag="PLG42_07295"
     CDS             4876..>5719
                     /locus_tag="PLG42_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOU31052.1"
                     /translation="MKHLKSYLVISLIIAGVINVCAQDWPQIFGPNRNNISPQKGLLR
                     SWPEKGPQLLWSVDVGPGYGGPVVKDGKVYLLDRVDDAKEIMRCFDLNTGKELWKFEY
                     DAPGKVDHPGSRSVPTADGNYVYSVGHNGDLYCFDVNTHKPVWQKNLMKDFGGTKLPV
                     WAITQSPLIYGDLLIVAYSKDPYAGLVAYNKRNGNIVWQTDAIGNETYASPAIAKIGG
                     EDHIVMVFSSTNTFMHRNIPTSSGKIIGLKPQTGAILWEYNNWTNVIQIPLALDIGDG
                     RIIVV"
BASE COUNT         1694 a         1107 c         1165 g         1753 t
ORIGIN      
        1 acggaatctg agctgtaaag ttaaaataat aatcaaacag ttgacattta tttttaccta
       61 ccaacatcca caacggcatg tgcgggtgtc gtactgatgg cagaatgggc atattttagc
      121 ccagaaccag actatgctga gcaaggcata gattatgtaa atgactgcca gagtaaaaga
      181 taattccttg ataatgatga aaccgagtat aaaaagaatc aacgtcggta cattatccag
      241 taaaataagc cattgagggg tttcttccgt atggcagtta tttatgttat tccgcattat
      301 gtggttgaat gtttcaacgt gaaactttta tgcagtagag ttttgtctgg tcccgcacaa
      361 gcaatttacc gccggcgagg gcaagtgctg cccagttttg tgcgctaccg tatctggctg
      421 ccgtatcttc gttagttgcc ggaatacctt cattaagaat cacggtttca gcaacgggct
      481 tgaattttgt tgaatcaggt tcaataaggt aaagtttggt cattccgtcg gtacagagga
      541 gtagcccgtc ggcgagaatc atgccgccct tgtcaaacag aggttctctg ccggttttcc
      601 atttcacctg cccattcatg ctcatgcaca ccaatccatc ttttctttcg tttgtcgagt
      661 actgggcata gaagtatccg ttataaagga taggagcctg ggtgtgagca ccaaaatctg
      721 gatttttata taattcagtt acttcgtaag aaccgtctga ctttttctgt acttttatca
      781 tagcggagcc tgcctcgtaa gcacctgtta taagtatcct tccttcgccg gcgtcaaggg
      841 cgcatggtac cggtatcctg catgaccacc ccttgtattc ccagagtact ttcccgcttc
      901 gtggatctat tcctgcaaca acacttggag tggccgattc acccctgccg gctgaagcac
      961 ttatcataac aacgtgattt tcgttgccta tttttacaat gacagggctt acatacccag
     1021 ctggccctaa agaaggagtc ttccatttta aattgcctgt gagtttatca taagccacaa
     1081 ctcctgcctg gggagcctgg gatgcgataa taagaaggtc tccatgtacg agcggacact
     1141 gtgtaattgc ccagcgggga atctgaccac caccgaaatc cgtccagatg tttttgttcc
     1201 ataaaggttt gttggtattt acatctatgc agtaaagatg tccataaggc ccgcatgtgt
     1261 atatcctgtt tccatctatt gccggtacac tccgtgaacc ggggaactgc acagatcccg
     1321 gagcatcata actgaaactc cagagctctt ttccgctggc aaggtcgaag cacctaagct
     1381 tgtcgcccac cttatcgtcc ctgtcgagaa ggtatacctt gccgtcctta actacaggcc
     1441 cgccaaagcc gatgccaacg tcaacactcc acaatacttc cggcccggtt ttaggccatg
     1501 agcgtaaaat gcccttttcg ttcgagatgc catttcggcc gggcccacgg tactgtggcc
     1561 agtcctggca atagagattc attatcgaag ctgaaagaat aatcaacgaa atcagaattt
     1621 tgaattgttt catggcataa acttttaatt tttggctatc agatttttat tcttttaaag
     1681 ggcccctgcg ccccgtccgg taaacattac agcagggatg cctctaaatt aatttttaat
     1741 taccaagttc aattttaatg taattaattt ttttactttt tcgatgaatc ttttcatatt
     1801 tcagaacaat ccatgccaac agcatggtac ttttcatgtt aatttttaat atctacatgc
     1861 ttagtatttt tctttcgata tactccttat tgaatgaata taccacactc acgttccata
     1921 caaaatcttt atctgcgtgt ataaacgtat tgaaaggcag actcatcatt gatgacaccg
     1981 ttaaaggaaa attctttctg acaagagtaa tcgccgaagt aaaatagtat cccttcattt
     2041 catccatttt gagaaaatat atttgaggat taaaccttaa gaaataattt ccggaaaggc
     2101 caatatgtga aaaattaacc ctcagagcaa gaaaatttgt attttttatt gcgtcgtttt
     2161 cgagacaaaa tgcatataga taatagatac cggtgttaat tgattttgtc agattatatg
     2221 aaggatagaa ttcgcctgcg aggtagcgcc ttacgatagt cagttttttt gggttaccgt
     2281 ctgctgtaaa agattctgtt ttaaaagcca gtgcgggatg tgtgccgatg attattcgaa
     2341 atctctcatt ttcaagtagt ttataacgcc accagaaaat aaaagtccat ggcttgcctt
     2401 cgcaggcgaa cctgagctga ggctcaaaac tgagttttct tttgcctacc gacatatcaa
     2461 atataactgc aggtttgcca agagtaagat ttggtatcag cgaaattccg ttattggtta
     2521 aacttaccgc tccgccgaaa tgacctgcaa tcagtgtact gtcagtcttc tgagcaaaag
     2581 tgataatata tgacgacatt gccagtaacg caaacattaa atatgaaatt ttgcctttca
     2641 tctttcaaat tttatctgat aagttcaatt tgagcccgca acccaaaact cccattcttt
     2701 cttgaatgtt aaaatgcaaa ataattacat cagaaacaaa gattacaaaa ttcaaacaaa
     2761 taataacttc ccggtattcg ttattatgcc tttctttttc tttttgagac ttcaccggtt
     2821 tatttatctc tcttaaatta tttattttga aggctgaaaa aattattttt taatccactt
     2881 atcggagatt ttataaggaa ttttaattca tgaaaaaaaa ttattgaagt tggttgaaaa
     2941 caatatcccg gagagagaga tgaagagggg ttttaggagt cactctctga taaagcttct
     3001 ggttgtgggc atgatcagtg ctgtttttgt gctttacccc aatattgctt ttttttcatt
     3061 tgagagagac tatcttgggg aggccaaata tgcagcatat ttgtttttct ttattttcag
     3121 gttcgtttac ttttcgcttc tgatatgggt acttcttgtt tacaatctca gaaaattaaa
     3181 aacacctttt ttaaccaaac gtctcattta taccttcttt atcacagcag cgggatatct
     3241 ggtctatatt gtgttttcaa tcctgttgag tcctaagcct gagtggtact caggtctgtt
     3301 gctgtttcag ttttttgtga tgttcatatt ctgtggtctt atagggcatg tttttcatct
     3361 atatgaggag cagcacagaa aagagcagga aattgaaagg ctaaaacacg aaaaccttca
     3421 aagtcgttat attgcacttg ccaatcagat taatccgcac tttttcttta attcactgaa
     3481 cggacttgtt tccgtgatcc gtaaaaaaaa tgaagaaaat gccattgaat atgtaaacaa
     3541 actatccgat gtattcaggt acattcttca aagtgacagg aaaggactgg ttttgctcga
     3601 ggatgaactg gaattcgtaa gggcatttca gtatatgatg gaggtaaggt ttgccagtaa
     3661 actggtattc agtataaatg taagtgatga aatgaaagct ctgaaactgc ctgtactttc
     3721 tctccttcct ctgattgaaa atgttgtttt gcataataca attgacagcg aacaccatat
     3781 ggaagtggat atttacgtta attctgagag agagctggtg gtctcaaatc ctgtatatcc
     3841 aaaactatcg ccacctgaga ctaacggtac gggtcttaaa aaccttgaaa atcgctttat
     3901 tttattgatg aataagcata taagggtaga aaaagataac ataagattta cagtttattt
     3961 acctctgaaa agtaaaaacg atgaggatac tgatagttga agatgagaca gcagcttatg
     4021 aaaacctgct gagtgttatt aaaaaaattg accctgaaat tgaaattgca ggttgtgcag
     4081 aaagtgtcag gcagaccata cgctggctga gagccaaccc aatacctgac ctgatactta
     4141 tggacatcca cctgtcagat ggttctgctt tttctatttt cgacagtata aatgttgaat
     4201 cgccggtgat tttcataact gcctatgacg attatgcaat cgaagctttt aaagtcaaca
     4261 gtatcgacta catactcaaa cccattaaag aagaggattt aagaagggca ctcgagaagt
     4321 ttaaaaagct cactcatcat gatattctta aatatcttgc gcagatgaca caaatggttt
     4381 cgcttccggt gtataaagat aaattattga ttccggaaaa agataaactg attccggttg
     4441 atatcaacga aatatcgtgc ttttattcta ctgaaaagaa taccagggtg ttcctgaaga
     4501 acggtaaatc gtaccagtgg aacagatcac tcgaacagat tgaagaggga ctcaaccctg
     4561 ctgattttat aagagcaaac aagcaatata ttatctcgag aagcagtgtt aagagtatta
     4621 ccgtatggtt tgataaccgt cttcttatca ctcttgatac ggaaacacct gagagaattt
     4681 atgttagtaa aaatagagca gctgaattca aggcatggat gatgcatggg aagaaatagc
     4741 agattattgt ttcgtgatac ttcctgattt ttcactttta aggtaaatct atggttgcgt
     4801 attaatttta ttgcaatatt tgtgttgaag aagttgatat ttaacggttt aataaaaact
     4861 taaaaaaatt tgaaaatgaa acatttaaag agctatctgg taatcagcct gattattgca
     4921 ggtgttatta atgtttgtgc ccaggactgg ccccagattt tcgggccaaa caggaacaac
     4981 atatctcctc agaagggtct tctcagatcc tggcctgaaa aaggacccca gcttttatgg
     5041 agtgttgatg taggtccggg ttatggtggt cctgtagtaa aagacgggaa agtctatttg
     5101 ctcgacaggg tagacgacgc aaaagaaatt atgagatgct tcgatctgaa caccgggaag
     5161 gaattgtgga agttcgagta cgatgccccc ggaaaggttg accaccctgg ctcaagatcg
     5221 gtgcctactg ctgacggtaa ttatgtatat tctgtcggac acaatggtga tctttattgt
     5281 tttgatgtaa acacacataa gcctgtctgg cagaaaaacc ttatgaagga ttttggcggc
     5341 accaaactgc cggtatgggc aataacccag agcccactca tttatgggga tctgcttatt
     5401 gtggcttatt caaaagaccc gtacgcagga ttggttgcat ataataaacg taacggaaac
     5461 attgtctggc agacagatgc aataggcaat gaaacctatg caagtccggc aattgcaaaa
     5521 attggaggcg aagatcatat cgtaatggta ttttcatcaa ccaatacgtt tatgcacaga
     5581 aatataccaa cttcgtcggg taagattatt ggtctgaaac cccagacagg agcaattctc
     5641 tgggagtaca ataactggac gaacgttatt cagattcctc ttgcacttga cattggagac
     5701 gggagaataa ttgttgtag
//
LOCUS       DAOSNB010000162         5687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14347_length_5687_cov_5.302557, whole genome shotgun sequence.
ACCESSION   DAOSNB010000162 DAOSNB010000000
VERSION     DAOSNB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14347_length_5687_cov_5.302557"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLG42_07300"
     CDS             complement(<1..331)
                     /locus_tag="PLG42_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31053.1"
                     /translation="MKEKNNNLIGRIQQYISKTKGPGFYRADDRVYHPFNVIVQKEIS
                     DHVRSWRFIILLVIISLTCLGSMYTAMSSIKETIKPNDPEGSFVFLKLFTSSDGILPS
                     FVVFLGFL"
     gene            complement(657..1307)
                     /locus_tag="PLG42_07305"
                     /pseudo
     CDS             complement(657..1307)
                     /locus_tag="PLG42_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1311..2456)
                     /locus_tag="PLG42_07310"
     CDS             complement(1311..2456)
                     /locus_tag="PLG42_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HOU31054.1"
                     /translation="MSIRIKESLIFILILTATTFNLKADEADSVKLFTPYPRVSVSPG
                     ESLDYTITVINNSSEIKTIDVSVTGIPKSWNYTLKSGTWNVKQISVMPKEKQLISLRI
                     DVPYNVNKGTYRFNFIASGYGTLPIEVIVTQQGSFNSEFTTDQNNLQGTPSSSFTFNA
                     TLRNRTPEKQLYAFSAYAPRGWIVTFRANYQAVTSVNVEPNANQSVTIEVKPPERVEA
                     GKYKIPVTASTGNSSASLELEVVITGTYGIEVSTPTGLLSTKMTAGEEKRIDLIVRNT
                     GTAELTGIALNYSGPVNWSCDFEPKKVDKLAANDTARVVAKLKADKKAIPGDYVAYID
                     ATTPEATSKITYRVTIETPLLWGWVGIAIIAAAIVSVYYLFRKYGRR"
     gene            complement(2716..2907)
                     /locus_tag="PLG42_07315"
     CDS             complement(2716..2907)
                     /locus_tag="PLG42_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOU31055.1"
                     /translation="MKKGTVKFFNESKGFGFIRDEDSLKEYFVHASGLLDVIRDNDEV
                     TFDLEMGKKGLNAVNVKLA"
     gene            complement(3230..3303)
                     /locus_tag="PLG42_07320"
     tRNA            complement(3230..3303)
                     /locus_tag="PLG42_07320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3267..3269),aa:Pro,seq:cgg)
     gene            3432..4622
                     /locus_tag="PLG42_07325"
     CDS             3432..4622
                     /locus_tag="PLG42_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31056.1"
                     /translation="MVHTDRIGAVDVAKGIALLVIVALTDYSPGIINDWFSSTLETPF
                     LNILATLSFGTFFYLYGITIPFYVSKKINEGHSSPEIIRMIFARTLILLVVGMMLSNY
                     DRVNSGLTGFSGSLWLVLLVIAIFLVWNRYPEREDIFFTVSGLRILGLAILVFLVLKF
                     NSGSFENSGSLIPGSWELPGMLGWGFLISALIWLALRNSIAGTLLALLFFLALNIIGA
                     LGLNPYLDPARKYAGIIIDGFIPAIILAGHTTGVLLKRYPESEYPKLSLVILFIAIIF
                     IVSGIFTLKFFPEGTTFNNPAWVLTSTGAASMLFVILLWLTDLRKTPLRLPVTETAGK
                     NFITAYILFFLVSGLISLSKLNILIYKTSDSLLIKTAGSVFLSFVIIFITSSLARVGI
                     RLKF"
     gene            complement(4627..>5687)
                     /locus_tag="PLG42_07330"
     CDS             complement(4627..>5687)
                     /locus_tag="PLG42_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOU31057.1"
                     /translation="ADSYLKGIRGYNIDTLYKAIIKYTFGPGPVSSVGRLGADYYNRL
                     GYIPYDAGIRESVARTLEYAYADFCIWKLAKALGRPQEEIKLYEGRAMNYKNVFDPER
                     KLMRGRNEDGTFQSPFNPFKWGDAFTEGNSWHYTWSVFQDIEGLVQLMGGREAFIAKL
                     DSVFNLPPIFDYSYYRQVIHEIREMQIMNMGNYAHGNQPIQHMIYLYNYAGQPWKAQY
                     HVRDVMDRLYNYTPDGYCGDEDNGQTSAWYVFSALGFYPVTPGTGQYVFGSPLFRKAT
                     IRFENGRVLAILAPKNSKTNVYISKIKLNGKKIKRNYITHLELQKGGKLVFRMSSEPE
                     KKRGTDEKAYPYSMSREK"
BASE COUNT         1549 a         1300 c         1079 g         1759 t
ORIGIN      
        1 caagaaatcc gagaaataca acaaatgatg gcaaaatacc gtcggacgat gtaaatagtt
       61 tcaggaaaac aaatgaacct tcagggtcat tgggctttat cgtctccttg atgctcgaca
      121 tggctgtata catcgagcca aggcatgtaa gtgatataat aaccagcagt attataaacc
      181 tccagcttct aacatgatca gaaatctcct tctgaacaat aacgttaaaa ggatgataga
      241 ccctgtcgtc tgccctatag aaaccgggtc cttttgtttt tgaaatatat tgctgaattc
      301 tgccgataag gttattattc ttttctttca ttcttttttc ctccttcaaa ataccggtaa
      361 taaatctcat ccaggccgta ctcttttcgg ttaagataat acaattgtgc gcctgattct
      421 actatagctt ttgctatttc ccctgtcgcg tcgcgcgtac agtgtattct gaacagatta
      481 ccgtttttgc ttactgcctg tactcctact acagaggcaa ggattctttt tacccactcc
      541 tcctgcgcct tattgtcagc cagctgcaag cctgatgagt cgaagcctat tccggcttca
      601 atcacaaagg gattaccttc aaacagtacc tgcgacaatg tcttcaggtc tccctgggcg
      661 agcaacttcc cgccgacaaa tattccgacc ctgtcacata ttttttgtac ctgatgcagg
      721 tcatgggatg aaagcagcac tgttatacct tcctgcctgc tcaatcttgc aataagctga
      781 agaaactcca ccacccctgc cgggtcgatt ccaagcgtgg gttcatcaag gattattact
      841 tcgggattct ttatcaatac ttctgcaaga cctagtcgtt gcagcatacc ccgcgaatat
      901 ttgcctgttt ttttccccgc agcctctgta agccctacct tttccagcaa aagctccact
      961 ttcatggacg cctccttccc cgacatcctg ttcaggcgtg cggtaaacat aaggttttcc
     1021 catccgctga ggttatagta aaatcccaca tcttccggaa ggtaacctac tttctttttg
     1081 acttcaatgg gatttgtgac aggatctata ccgcacactc ttaccgaacc ggaagtgggc
     1141 tctgtaagcc caagaagcat gagtatggtg gttgattttc ctgcaccgtt aggtcccaga
     1201 agaccgaata cttctccttt acggatagtc agatcaagct ggtcaaccgc cgtaaatgac
     1261 ccgtacttct ttgtgagatt aaccagttca ataatgtttt cgttcacggt ttacctcctt
     1321 ccgtatttcc tgaacaggta atatacgctt acaattgctg ctgctattat tgctattccc
     1381 acccagcccc aaaggagcgg agtctctatt gtaaccctgt aggtaatctt cgatgtagcc
     1441 tcaggtgttg tggcatcgat gtaggcgaca taatcacccg gaattgcctt tttgtcggct
     1501 ttaagcttag caacaaccct ggcagtgtca ttggctgcaa gcttgtctac cttcttcggc
     1561 tcaaagtcac acgaccagtt gacaggccca gaataattta aagcaatacc tgtaagctct
     1621 gcagttccgg tgtttcttac aataaggtcg attctttttt cctctcccgc agtcatcttg
     1681 gtgctcagaa gtccggttgg tgtcgacacc tcaatgccgt aggtacctgt aatgactacc
     1741 tcaagttcca gggaagccga actattacct gttgatgctg ttacaggtat tttatatttg
     1801 cctgcctcaa ctctctcggg tggtttgact tcaattgtaa ctgactggtt tgcattaggc
     1861 tccacattaa ccgaagtcac tgcctgatag tttgccctga atgtaacaat ccacccacgt
     1921 ggtgcatatg ctgaaaatgc ataaagctgt ttttcagggg tacggttgcg aagtgttgca
     1981 ttaaaagtga agctggaact cggtgtgccc tgcaggttat tctggtcagt agtgaattcg
     2041 ctgttaaaac tcccctgctg ggtaactatc acttcaatag gaagagttcc atatcccgaa
     2101 gctatgaaat tgaaacggta tgtgccttta ttaacattgt acggtacatc tattctcagt
     2161 gaaatcagct gtttctcctt tggcatcact gaaatctgct ttacattcca tgtgcctgat
     2221 ttgagggtat aattccagct cttcggaata ccggttactg aaacatcaat agtctttatt
     2281 tcactgctat tattgataac agtgatagtg taatcgagcg attcacccgg tgaaactgat
     2341 accctcgggt acggagtgaa aagctttaca ctgtctgcct catctgcttt taagttaaaa
     2401 gttgttgctg tcagaattaa aataaaaatc agagactctt taatacgtat tgacatatca
     2461 attcctcctt gtaaaatttt ttattttttt cttaaacagg ggcaaaataa gagaatttac
     2521 ttgaaataac agcccccttg catttattgc taataattta gcgacagcaa atttatttta
     2581 attgcggttt ggatttttta aagaaatttt aaagaattgt taaaaaaagc aaaaaaccgc
     2641 atcaaatgag ataattaaaa taaaaagtcc cgcttaaagg cgggactaaa aaatttaaat
     2701 gactataata gttaactaag ctagtttaac atttactgca tttaatcctt ttttacccat
     2761 ttcaagatca aaagtcactt catcgttatc cctgatgaca tccagtaatc ctgaagcatg
     2821 tacaaaatac tccttaagtg aatcttcatc tctgataaaa ccgaatcctt ttgattcatt
     2881 gaaaaattta actgttcctt ttttcatgat aaaataaaat tattattaat gtgcgacaaa
     2941 ggtacgttta ataaatggaa taaaaaatta attctcaata tatttagttt ccggcagaat
     3001 gtttaatacc tgccgggtac cttaacaaac atgtttattt actggagctg tgctttaaag
     3061 aaacaataaa catattcaaa taaaatgcaa aaaaaaatgc aatcactaat tgctgaagtg
     3121 attgcttttt tattttcccg ggtaaaaaga ttccccttct gagcaactga ataccgttct
     3181 gccaggcagg ggaatagtta agttcgtgcc ccgaacaccg gtaaatctgt cggggtgact
     3241 ggactcgaac cagcgacctc cacgtcccga acgtggcgcg ctaaccaact gtgctacacc
     3301 ccgcacttaa cagttcagcg cacaaaagta ataaaaattt ctactcttaa agccgtgcgg
     3361 attatttata tacaaccagt cgtgaactac aagcaattta tttcgtattt ttgggcactt
     3421 tatgccagca aatggtacac accgatcgca taggagcagt tgacgtggca aagggaatag
     3481 ctctgcttgt aattgtagcc cttaccgact attcaccggg aattataaac gattggttca
     3541 gctcaaccct tgaaacacct tttttaaaca tcctcgcaac actttcattc ggaactttct
     3601 tctatctcta tggaataaca attccttttt atgtttcaaa aaaaatcaat gaaggacatt
     3661 caagtccgga aataataaga atgatctttg cccgtacact tatactcctt gttgtgggta
     3721 tgatgctttc aaattacgac agggtcaatt caggccttac cggtttcagc ggctcactgt
     3781 ggctggtact gcttgtaatt gcaatatttc ttgtctggaa cagatacccc gagagggaag
     3841 atattttttt cacggttagc ggattaagaa tcctcgggct ggcaattctt gtttttctgg
     3901 tgctgaagtt caattccggt tcgtttgaaa acagcgggtc actgatacct ggtagctggg
     3961 aacttccggg aatgcttgga tggggatttc tcatatcggc actgatatgg ctggcgctac
     4021 gaaattcaat agcaggaaca ctcttagcct tactattttt cctggcactg aacataatag
     4081 gagcacttgg actcaacccc tatctcgacc ccgcaagaaa atatgctggc ataataatcg
     4141 atgggttcat ccctgcaata attttggccg gacatacaac tggcgtactg cttaaaagat
     4201 accccgaatc ggaataccca aaactatcat tggttattct ttttatcgca attattttta
     4261 ttgtttccgg aatttttacc ctaaaatttt ttcctgaagg aacaactttt aataatcctg
     4321 catgggttct gacctcaaca ggggctgctt caatgctttt cgttatactg ctgtggctca
     4381 ccgacctgcg taaaactccg ctacgtttac cggtaaccga aacagcgggg aaaaacttta
     4441 tcactgccta catactcttc tttctggttt caggtctgat aagtctcagc aagcttaata
     4501 tccttattta caaaacatcc gattcactat tgataaaaac cgccggatca gtttttcttt
     4561 cctttgtgat tatcttcatt acttccagtc tggcaagggt tggtatcaga ttgaaattct
     4621 gacaggctat ttttcgcgac tcatcgaata aggataagcc ttctcatccg tgcctctttt
     4681 cttttcaggt tcagagctca ttctgaaaac cagctttcct cctttctgaa gctccagatg
     4741 ggtaatgtaa tttcttttta tcttcttacc gttaagtttt attttactga tgtacacgtt
     4801 tgtctttgag tttttcggag ccagaatagc aagtacccgt ccgttttcga atctgatggt
     4861 tgctttcctg aaaagtggcg aaccgaacac atactggccg gtaccagggg tgacaggata
     4921 aaagcccagt gctgaaaaga cataccatgc cgaagtctgc ccgttgtctt catcaccaca
     4981 ataaccatcg ggtgtgtaat tgtaaagcct gtccattaca tcccgtacat gatactgggc
     5041 tttccatggc tgacctgcat aattatacag atatatcata tgctgaatgg gctggttacc
     5101 gtgagcatag tttcccatgt tcataatctg catctcgcgt atttcgtgga taacctgcct
     5161 gtagtaagaa taatcaaaaa tcggaggaag gttgaacact gaatcgagtt ttgcaatgaa
     5221 agcttcacgt cctcccatca gctgtacaag tccttcaatg tcctgaaaga cactccatgt
     5281 atagtgccag ctattgcctt cggtaaaggc atcaccccat ttgaatgggt taaacggcga
     5341 ctgaaacgtt ccgtcttcat tccttccccg catgagtttc ctctcagggt caaaaacatt
     5401 tttataattc attgccctac cctcatagag ctttatttct tcctgcggtc tgcccagtgc
     5461 ttttgcaagc ttccagatgc agaaatcagc atacgcatac tcaagtgtac gcgccacact
     5521 ttcccttatc cctgcatcgt acggaatata tccgagcctg ttataatagt cggcaccaag
     5581 cctgcctaca gagctgaccg gacctggtcc gaaagtatat tttataattg ctttataaag
     5641 agtatcaata ttgtatcccc ttattccttt gaggtaggaa tcggcaa
//
LOCUS       DAOSNB010000163         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14395_length_5673_cov_4.617480, whole genome shotgun sequence.
ACCESSION   DAOSNB010000163 DAOSNB010000000
VERSION     DAOSNB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14395_length_5673_cov_4.617480"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLG42_07335"
     CDS             complement(<1..766)
                     /locus_tag="PLG42_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOU31058.1"
                     /translation="MKLLRHFICRKDERFPSKKMIIEFIRNVTTFLYPVLAEMDNPCR
                     SPRKVKGSLCRILSSVRANLAQPPEIIADRFMDALNGIKQQVDADASFIMEGDPAATS
                     LDEVIITYPGFYAILVYRLANKLALLEVPLIPRIMTEYAHSLTGIDIHPKATIDSPFF
                     IDHGTGVVIGETSVIGRRVKLYQGVTIGALSVSKDMAGKKRHPTIEDDVVIYAGSTIL
                     GGETVIGHDSIIGGNVWLTSSVPPYSLVYHENVMKIR"
     gene            complement(801..1577)
                     /gene="trpA"
                     /locus_tag="PLG42_07340"
     CDS             complement(801..1577)
                     /gene="trpA"
                     /locus_tag="PLG42_07340"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOU31059.1"
                     /translation="MNRIDKLFHSGKKNILSVYFTAGYPEVDSTAGIINSLVRAGVDM
                     IEIGIPFSDPLADGPVIQKSSSNALKNGMSLRLLFHQLESIRSSVNIPLLLMGYLNPV
                     LKFGMENFCRSCSVTGIDGIILPDLPPEIYTEQYREIFRKYGLYNILLISPRTEAERI
                     RLIDSITDGFIYMVSSSSVTGVKQGFNDEQKAYFERVAGMNLTHPLMIGFGVSSNETF
                     AEACRYANGAIVGSAFIKMLEKEGYSDENIKKFVKDLKKE"
     gene            complement(1580..2794)
                     /gene="trpB"
                     /locus_tag="PLG42_07345"
     CDS             complement(1580..2794)
                     /gene="trpB"
                     /locus_tag="PLG42_07345"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOU31060.1"
                     /translation="MRYNVDGKGFYGEFGGAFIPEMLYANIRELQENYLRIIESEDFR
                     KEFKYFLKNYAGRPSPLYFARRLSEHYGTQIYLKREDLNHTGAHKINNTIGQILIAKR
                     LGKTRIIAETGAGQHGVATATVCALMGLKCVVYMGKLDVERQAPNVLRMRMLGAEVIP
                     VESGNMTLKDATNEAIRDWISNPADTYYLIGSVVGPHPYPDLVSRLQSVISEELMFQL
                     QEHVGRNYPDFIIACVGGGSNAAGSFYHYLDNHDVKLVAVEAAGKGIGTGETAATIAE
                     GTPGIIHGSRTILMQNDDGQITEPYSISAGLDYPGIGPQLAWLGKTGRVKFEYATDDE
                     ALEAAFRLMRFEGIIPALEPAHALAALGKIKFSENDIVVVILSGRGDKDMASYIKYLE
                     NRSCKTPVNINM"
     gene            complement(2778..3416)
                     /locus_tag="PLG42_07350"
     CDS             complement(2778..3416)
                     /locus_tag="PLG42_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOU31061.1"
                     /translation="MIKVKVCGLKDPENVKKICETGIDFAGFIFSTKSPRYIGKNPDR
                     NIFGSIPDTIGRVGVFVDEEHDKIIETAKSLGIGIIQLHGNEDLSYCGKIKASGLQVI
                     KVFRVGNKIDLNHVNAYSDVCDYFLFDTGGRLYGGSGKKFNWDILNGLSLQKPFFLSG
                     GIGPGDVQELGKIKNDMFFGVDINSRFEISPGIKDFEKVEVFIKKIKYEIQC"
     gene            complement(3450..4235)
                     /gene="trpC"
                     /locus_tag="PLG42_07355"
     CDS             complement(3450..4235)
                     /gene="trpC"
                     /locus_tag="PLG42_07355"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOU31062.1"
                     /translation="MNFLEQIAEMKKQEIAQMKRLISATDYEKRKRIGREIVSLSDAL
                     KNSPTPGIIAEFKRMSPSKGVLNHQAKIADVVKGYEEGGAAGLSILTEKKYFGGSIGD
                     ISMVRLSTGLPILQKDFVIDEIQIYEAKYSGADAILLIASLLETNEVYRLARTARKTG
                     LEVVLEIHSEAELDKVNEYVNIIGVNNRNLLTLEVDTNTSFKLAGKIPEGFVKISESG
                     ISSPEVLKDLIKAGYDGFLIGEAFMSRPDPVKAFKEFVKMVKE"
     gene            complement(4232..5227)
                     /gene="trpD"
                     /locus_tag="PLG42_07360"
     CDS             complement(4232..5227)
                     /gene="trpD"
                     /locus_tag="PLG42_07360"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOU31063.1"
                     /translation="MRKILNYLFEHKTLSRPEAEEVMINIAGGKYSEAEIAAFITVYL
                     MRSITVEELSGFRNALLNLCNRVDLSEFDTIDVCGTGGDNKETFNISTLTAFVLAGAG
                     IKVAKHGNYGVTSACGSSNILEHFGYKFSTDTGKLKRELERAGLCFLHAPLFNPAMKT
                     VAPVRRALKVKTFFNMLGPTVNPSLPRRQLVGVYSLELARLYNYLYQETDVSYAVVHS
                     LDGYDEVSLTAPFRYIFNGREQILAPDELGYEAVQPMELAGGRTVAESADIFTRILEG
                     NGSEAQNNVVLANSQLAIRCCFPEKSPDECRKMAEDSLFNRKALDSFNTLIKMQQ"
     gene            complement(5275..>5673)
                     /locus_tag="PLG42_07365"
     CDS             complement(5275..>5673)
                     /locus_tag="PLG42_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOU31064.1"
                     /translation="GICIELIKRWAAEKSILGICLGHQAICEAFGGKLLNLSTVYHGI
                     SAPVKVKIPDEPLFKNIPPIFMAGRYHSWVVSRKHLPACFRITCEDDNGIIMGISHVE
                     YNVRGLQFHPESVMTEYGMNIIRNWLEIHK"
BASE COUNT         1529 a         1329 c         1066 g         1749 t
ORIGIN      
        1 ctctgatctt catcacattc tcatggtaaa caagcgaata aggaggcaca gaagaggtaa
       61 gccaaacgtt acccccaatt atgctgtcgt gcccgattac tgtttcacct cccagaatag
      121 tgctgcctgc ataaataaca acatcatcct caatggtcgg gtgacgtttt ttacccgcca
      181 tatctttgct aacgctcaga gctccaattg ttactccctg ataaagcttc actcttctgc
      241 cgataacaga tgtttcacct atcacaaccc cggttccgtg gtcaataaaa aagggtgaat
      301 cgatggtggc tttcgggtga atgtctatac ctgtcagtga atgagcatat tcggtcataa
      361 ttctcggtat gagaggcact tcaagcaatg caagcttgtt tgcaagcctg taaacaagga
      421 ttgcataaaa cccgggatat gtaattatca cttcatctaa cgacgttgca gccgggtctc
      481 cttccattat aaagcttgca tcggcatcaa cctgttgttt gataccattc aacgcatcca
      541 taaaccggtc agcgataatt tcaggaggct gagcaagatt tgccctgaca gatgaaagaa
      601 tgcggcagag tgaacctttt acttttcgtg gtgaacggca tgggttatcc atctcggcaa
      661 gaacaggata cagaaatgtt gttacatttc ggatgaattc tattatcatc ttttttgaag
      721 ggaacctttc atcttttctg caaatgaaat gtctgagcag tttcataata aaaattagtt
      781 taacaatctg gaatattgct tcattctttc tttaagtcct tcacaaattt tttgatattc
      841 tcgtcactat atccttcttt ttcaagcatt tttataaaag cgcttccaac aattgcaccg
      901 tttgcatatc tgcacgcctc tgcaaaagtt tcattggatg atacaccgaa tccaatcatg
      961 agaggatggg tcagattcat accagccact ctttcaaaat aggctttctg ctcatcattg
     1021 aaaccctgct taacacctgt gactgatgaa gaagaaacca tataaatgaa tccgtcggtg
     1081 atgctgtcaa tgagtcttat gcgttcggcc tctgtacggg gagatatcaa aagtatatta
     1141 taaagtccgt actttctgaa tatttctcgg tattgttccg tataaatttc cggtggaagg
     1201 tcgggcagaa taattccatc tatgcctgtt acagaacaac tcctgcaaaa attctccatc
     1261 ccaaatttta acactggatt gagatatccc ataagaagca gtggtatatt cacagaactt
     1321 cgtatactct caagttggtg gaataataac ctaaggctca tgccgttttt caatgcatta
     1381 ctgctgcttt tctgtataac cggtccgtcg gcaagggggt ctgaaaaggg gatacctatt
     1441 tcaatcatat ctacacctgc tcgaaccagg ctatttatta tacctgctgt tgagtcaacc
     1501 tcaggatatc ctgctgtaaa atacaccgaa aggatattct tttttcccga atgaaatagt
     1561 ttatctatac ggttcatttt cacatattta tattaaccgg tgttttacac gatctatttt
     1621 caagatattt aatataggat gccatatctt tatcgcctct tccagaaagg attacaacta
     1681 ctatatcgtt ttcactgaat ttaattttcc ccagtgctgc cagggcatgg gcaggttcga
     1741 gtgccgggat gattccttca aatctcataa gcctgaatgc ggcctccagg gcttcatcgt
     1801 cggtggcata ttcaaatttg actctgccgg ttttgcccag ccatgccagc tgtggtccta
     1861 tgcccggata atcgagtcct gctgatatgg aataaggttc ggttatctgt ccgtcatcat
     1921 tttgcatgag tatagtcctg ctgccatgaa ttatacccgg tgtgccctca gcaattgttg
     1981 cagcagtctc gcctgttccg attcctttcc ctgcggcctc cacagcaaca agttttacat
     2041 catgattatc caggtaatga taaaaagagc ccgcagcgtt gctgcctcct cctacacatg
     2101 caataataaa gtcgggatag ttcctcccga cgtgttcctg tagctgaaac ataagctctt
     2161 cacttattac cgattggagt cttgaaacaa ggtcggggta cggatgcggg ccaaccactg
     2221 atccaataag ataataagtg tcggcgggat tgctgatcca gtcacgtata gcctcattag
     2281 tggcatcttt tagtgtcata ttgccacttt cgacaggaat tacttccgct ccgagcattc
     2341 tcatcctcaa tacgttggga gcctgcctct ccacgtcgag cttccccata taaaccacac
     2401 acttaagtcc catgagtgca cacactgtag cagttgcaac cccatgttgc cctgcaccgg
     2461 tttcggcaat aatccttgtt ttacccagac gctttgctat cagtatctga ccaatagtgt
     2521 tattaatctt gtgagcacca gtatggttca ggtcttcacg cttaagatat atctgtgtgc
     2581 cataatgttc cgacaacctc ctggcaaaat aaagaggtga aggcctccct gcatagtttt
     2641 taaggaaata cttgaattcc tttctaaaat cttcactttc aattatcctc agataatttt
     2701 cctgcaattc cctgatattt gcataaagca tctccgggat aaaggctccg ccgaattcac
     2761 cgtaaaaacc tttcccgtca acattgtatc tcatatttga tttttttaat aaatacttcc
     2821 actttttcaa aatcttttat tcccggggag atttcaaacc ggctattaat gtcgacacca
     2881 aagaacatgt cgtttttaat ttttcccagt tcctgaacat caccgggacc tatgcctccg
     2941 ctcaggaaaa aaggtttttg aaggctgagg ccgttgagaa tatcccagtt gaactttttt
     3001 ccacttccac catagagtct gcccccagtg tcaaaaagga aataatcgca cacatcactg
     3061 tatgcattta catgatttaa atcaatctta tttccaactc tgaaaacttt tataacctgc
     3121 agacctgatg cttttatttt cccacaataa ctaagatctt cgttgccatg aagctggata
     3181 attccaatac ctaaagattt agctgtctca ataattttat catgttcctc atccacaaaa
     3241 acccctaccc tgcctatggt atccggtatg ctgccgaaaa tatttctgtc agggtttttg
     3301 ccaatatacc ttggagactt cgtggaaaag ataaaacctg caaagtcgat cccggtttcg
     3361 caaatctttt ttacgttttc ggggtccttc agtccacata cttttacttt aatcatctca
     3421 aatccctttc tggacaattc atgtcagttc tattctttta ccattttaac gaattcctta
     3481 aaagctttca ccggatcggg tcttgacatg aatgcttcgc caatcagaaa cccgtcgtac
     3541 ccggctttta taagatcttt cagcacttcg ggtgatgaaa tgccactttc ggaaattttt
     3601 acaaaacctt caggtatttt acctgcaagt ttgaaggatg tatttgtatc aacttcaagt
     3661 gttaaaagat tgcggttatt tacccctata atgtttacat attcattaac cttatcaagt
     3721 tctgcttcgg agtgaatctc aaggaccact tccagacctg tctttcttgc cgtccgggca
     3781 agccggtaca cttcattagt ctccagcaat gaggcaataa gcagtatagc atctgcccct
     3841 gaatatttag cctcataaat ctggatctca tcaattacaa aatctttctg aaggatcgga
     3901 agtcctgtag aaagtcgtac catcgaaata tcacctattg atccaccgaa atactttttc
     3961 tctgtcagga ttgatagtcc tgctgcaccc ccctcttcat aacctttcac aacatcggct
     4021 atttttgcct ggtggttaag aactcctttc gaaggcgaca tccttttaaa ctcggctata
     4081 attcctggtg tcggactatt tttaagggcg tcggaaagag aaacaatctc tctgccgatt
     4141 cttttgcgct tctcataatc agttgcggaa atcagtctct tcatctgagc gatttcctgt
     4201 tttttcattt cagctatctg ttccagaaaa ttcattgttg catctttatt aatgtgttaa
     4261 atgaatcaag tgcttttctg ttgaaaagtg aatcttcagc cattttcctg cactcatcag
     4321 gtgatttttc aggaaagcag catcttattg caagctggga attagccaga accacattat
     4381 tctgagcttc agaaccatta ccttcaagaa ttctggtgaa aatatcagcc gattcagcta
     4441 ctgtcctgcc tccagccagt tccatgggtt gaactgcttc atatcccagt tcatcaggag
     4501 ctaatatttg ttccctacca ttgaagatgt atctgaaagg tgcggtaagt gaaacttcgt
     4561 cgtaaccgtc gaggctgtgg acaactgcat aactgacatc tgtttcctga taaagataat
     4621 tatacaacct ggcaagctcc aggctgtaaa ctccaaccag ttggcgtctg ggcaatgacg
     4681 ggttgaccgt tggccccagc atgttgaaga aagtcttaac cttaagagct ctgcgcaccg
     4741 gagcaacagt tttcattgcg gggttgaaca agggtgcatg cagaaaacaa aggccagccc
     4801 gctcaagctc ccgctttagc ttgccggtat cggtcgaaaa cttatatcca aaatgttcaa
     4861 ggatatttga tgatccgcat gccgaagtca ctccgtaatt accatgtttt gcaaccttta
     4921 ttccggctcc agcaagaacg aaagcggtaa gtgttgatat attaaatgtt tccttgttat
     4981 caccgcctgt gccgcataca tcaatggtat caaactctga aagatcaacc ctgttgcaca
     5041 gattaagcag ggcattgcgg aagcccgaca gctcttcaac agtgatgctg cgcatcagat
     5101 aaacagtaat gaatgcagca atttcagctt cggaatattt gccgcctgct atgttaatca
     5161 taacctcttc agcctccggg cggctgagag ttttgtgctc aaagagatag tttaatatct
     5221 tcctcataaa tgtatcgtat aaattttcaa tatgtttaac aaattcataa tctgtcattt
     5281 atgtatctca agccagttcc tgattatatt cattccgtat tcagtcatta ccgattcggg
     5341 atggaactga agccccctga cattatattc gacatgactt atgcccatta tgatgccgtt
     5401 gtcatcctcg caggttattc tgaagcaggc aggcagatgt tttcgtgaga ctacccatga
     5461 atggtatctt ccggccataa aaattggtgg tatatttttg aaaagaggtt catcaggaat
     5521 cttcaccttt accggtgccg agatgccgtg gtacacagtg ctaaggttga gcagttttcc
     5581 tccaaaagcc tcacaaatag cctgatgtcc gaggcatatc ccgagtatac ttttttcagc
     5641 agcccacctt ttaatcagct caatacagat acc
//
LOCUS       DAOSNB010000164         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14396_length_5673_cov_3.652189, whole genome shotgun sequence.
ACCESSION   DAOSNB010000164 DAOSNB010000000
VERSION     DAOSNB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14396_length_5673_cov_3.652189"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <248..1348
                     /locus_tag="PLG42_07370"
     CDS             <248..1348
                     /locus_tag="PLG42_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU31065.1"
                     /translation="AQASKFLLDFGQFDSLSALEGGLFVDDEIQLSEKLAVTAGLRFS
                     FFSHHGPYEQLVKNSADLVTDTIHYPWNESLAFYKNLEPRAVVNYMIGENSSVKASYM
                     RIAQYVHLATSAAVTLPTDVWIPSTSGIKPQIGNQISLGYFGNILNYNYEFSSELFYK
                     RMNNKLEFLRGIIFNSIFGTFEDNLVRGFAQSYGIELFMRKKSGRFNGWISYTLSKTE
                     QKFKEINNNGFYPAKYDRRHDIAMMLNFEINKKWNCSGVFIYASGNAFTIPVGRYIIQ
                     GNIVNQYGKVNEFRMPPYHRLDLSLNRKIIVRQKYMSELNISVYNIYNRVNPYFLYFQ
                     ATGELETYSLNIKALQVSFSPIIPSLSWNFSF"
     gene            1354..2223
                     /locus_tag="PLG42_07375"
     CDS             1354..2223
                     /locus_tag="PLG42_07375"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOU31066.1"
                     /translation="MEPFIRYAILFFIIQVFISCNKVPDYSIQDPDDLIVIDGWIENG
                     QYAKVLLTKNAPYFSLLDSTSLRELVLTRATVSLSDGERQEYLILRRNKDYFPPFIFE
                     GNEIKGEPGKTYTITASYGGKTAIGKTSIPPPVSLDTIYFKMKENSDSLGSLWIEFTD
                     PPDTRNYYRILSKIVGRDRRYYSAMIMGLSDESFSGKKFGFSIFRGQDSYISVNKNSY
                     FSLGDTVNLKFCTIDKAHYEFWNSFQDEILNSGNPFASSLTVVKTNIEGDGLGIWGGY
                     GVSFYSVVIKNAP"
     gene            complement(2458..3837)
                     /locus_tag="PLG42_07380"
     CDS             complement(2458..3837)
                     /locus_tag="PLG42_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31067.1"
                     /translation="MKNLFKSVLIALLVYSMLSCSSDKEKTESFTTRSVEENKATIES
                     AGIDLIGVLNRMRSTQTVEAIGNMIQLTGSGKSKGIGDFTGTGLYSVFRSIHGCAART
                     LQVNDVFESIKGCADEDPESIQEFWDRNVGTYTWNSSASDWDISPGGDKFIIKFPSSG
                     NSKMNNATFTIYNYEGVIVENPFDEEYTGDFPASLQADLKIGSQTLLRFVFGASYNAD
                     GIPNAIACDLDFEGFKFEIDVANDTRVISASYKFMEGKAVIMKLSAVVNGLFTADNLE
                     LNTVHHSESHSYVCDFVYNPETKEYVEVYCEYTNEWEKIEFEEIAHSANAEFQLMNLA
                     VRGDIDIKKLVDKIRIIEDQYDTEQIEEEEYFNSISEEINKYLNLRLVNINNNEILAK
                     VEAYVEHESDWGYEDFYVSFRLTFGEESPIDMETYFGEGFDDFIKSLNDLLEDICNDY
                     GIDYDPIDY"
     gene            complement(3919..>5673)
                     /locus_tag="PLG42_07385"
     CDS             complement(3919..>5673)
                     /locus_tag="PLG42_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOU31068.1"
                     /translation="GGDEIIARGICWSLSTNPTTTNNKTNSGTGTGLFIDHIGPLTPG
                     TTYYVRAYATNSAGTAYGNEEVFVTCDYIPPVAFNSNLAYGVITDIEGNFYKTIKIGN
                     QEWMAENLRTTKYNNGESIPVITDNDTWQSLSEGALCWLNDNICYKNTYGGIYNWYAA
                     NNNLCPVGWHLPTNEEWSVMNRALGGNEIAGGKLKEQGFSHWSEPNTGATNESGFSAL
                     PTGGFRNGALFVTGEFASFWTSTEYPDNQAFYRSLQHQYSIEGYSWGYKSNGANVRCL
                     KGPAPPKPSVTTLWARNIMSDKAEIVSVISSVLPVNSRGVCWNTSPAPEVLDNKTTND
                     GGTGSFTSLLTGLESNTTYYVRAYSINDDGITYGNEITFSTVEDLPIGGLAAYWPFNG
                     NANDESGNNNNGTVYGATLVADRFGNPEKAYYFNGISNKIYIGNSLIVQGHITVACWI
                     KSTDIGNQIISSGSRNSYRIGKTKDGVDAAVYLTDSSDGYGLDVHAVTDCSDWTHVAI
                     VYNGVSLDLYVNGQLMDSAYGPGNVFCNQPVYLNFGTYQWLSTYYGFYQGYLDDVYMF
                     NRALSNSEIEALYHEGGW"
BASE COUNT         1664 a         1134 c         1035 g         1830 t
ORIGIN      
        1 gcgggatctt ttcaatggga tcataaaatt cggaataatt cccggattaa taccagtttg
       61 tcattaacca actatgcctt cgatttgtcc ggatcgcagg atgaatattc ttttaaaatg
      121 ttttcttctg taagggatta taatttaaag agtgtacttg atattcagac gggaaaaaat
      181 aagttcagcg ccggttttga atttaccgaa catttttttc ttccaaacaa aataaatnnn
      241 nnnnnnngcg caggcgagta aattcctcct ggattttgga caattcgatt ctctttctgc
      301 attggaagga ggattattcg ttgatgatga aatccagcta tcagagaagc tggctgtaac
      361 tgccggtttg agattttctt ttttcagtca tcatggacct tatgaacaac ttgtaaaaaa
      421 ttctgcggat ttggtgacag atacgatcca ttatccctgg aatgagtctc tggctttcta
      481 taaaaatctg gaaccccgtg ctgttgtgaa ctacatgatc ggggaaaatt cctctgttaa
      541 agcctcatac atgcgaattg cacaatatgt gcatctggca acttccgcgg cagtaaccct
      601 cccgacggat gtctggatac ccagtacctc cgggataaaa ccacagatcg ggaatcagat
      661 aagcttaggt tatttcggga atattttgaa ttataattat gagttttctt cagaattgtt
      721 ttataaaaga atgaataata aactggagtt tttacgcggc ataatcttta actctatttt
      781 tggcactttc gaagataatc tggtgagagg atttgcccag tcttacggta tagagctgtt
      841 tatgaggaaa aaaagcggaa gatttaatgg ctggataagt tatactttgt ctaaaacaga
      901 acagaagttc aaagaaatca ataacaatgg tttctatcct gcaaaatacg acaggagaca
      961 tgacattgcc atgatgctga actttgaaat caacaagaaa tggaattgtt ccggagtatt
     1021 tatatatgct tccggtaatg cctttactat tcctgtaggc agatatatta ttcagggaaa
     1081 cattgtaaat caatacggaa aggtaaatga attcagaatg cctccgtatc acagactgga
     1141 cctctcctta aaccgtaaaa tcatagtaag acaaaaatat atgtcagaat tgaatatatc
     1201 agtttataat atttacaaca gggtaaatcc atactttttg tattttcaag caacgggcga
     1261 attggaaacc tattcgctta atatcaaagc cttacaagta agcttttccc cgataatacc
     1321 atcattaagc tggaacttta gcttttaggc gatatggaac catttataag atatgcaata
     1381 ttgtttttta ttattcaggt gtttatttcc tgcaataaag tgcctgatta ttctattcag
     1441 gatcctgatg acctgattgt tatagatggc tggatcgaga acgggcaata tgcaaaagta
     1501 cttcttacaa aaaatgcacc ctatttttcc ttactggatt caacatcact cagagaactc
     1561 gtactgacca gagcaacggt aagcctttct gacggggaaa gacaggagta cctgatacta
     1621 cgcagaaata aggactattt tccgccattt attttcgaag gaaatgaaat aaaaggtgaa
     1681 ccgggaaaga cctatacgat tacagcaagt tatggtggaa aaacagcaat aggaaaaaca
     1741 agcattcctc ctcctgtcag tctggataca atttatttca agatgaagga aaattcggat
     1801 tcccttggat cactctggat tgaatttacc gatcctccgg acacacggaa ttattacagg
     1861 attctttcaa agatcgtggg cagggaccgc cggtattatt ctgccatgat tatgggatta
     1921 agtgatgaat cgttcagtgg gaaaaaattt ggattctcaa ttttcagggg tcaggattcg
     1981 tatatttcag taaacaagaa tagttatttt agtctcggag atactgtaaa cctgaaattc
     2041 tgtacaatag ataaagctca ttatgaattc tggaactctt ttcaggatga aattcttaat
     2101 tccggtaatc cttttgcctc ttcacttact gtggttaaaa caaatataga gggagatggc
     2161 cttggcatat ggggaggata tggagtcagc ttttattcgg tggtaataaa aaatgccccg
     2221 tagccggggt tttcattttc gagattttta aaatcaggaa acaatggagc ctgcccattg
     2281 cttcctgata agtttatatg ctgcttacgg cattgataag ttacatttgt actttattga
     2341 atttttattc tgcttgagga ttcaacagga aacgactcaa taaatttgat tggatccatg
     2401 taaaatgttt tcctgtgaca ggatcaattt atttatcatt tcctgtattc atatttttta
     2461 ataatctatt ggatcataat caataccata atcattacat atatcttcca gaagatcatt
     2521 gaggctcttg ataaaatcat caaagccttc tccaaaatat gtttccatat caattggtga
     2581 ttcttcccca aaagtaagac ggaagctcac ataaaaatct tcatatcccc agtcagattc
     2641 atgctcaaca taagcttcaa cctttgccag aatctcgtta ttatttatat ttacgagcct
     2701 caggttcaga tatttgttta tttcttcaga tatgctgttg aaatattctt cttcttcaat
     2761 ttgttcggtg tcatactgat cctcaataat cctgattttg tcaaccagtt tttttatatc
     2821 aatatcacca cggacagcca gattcattaa ctggaactca gcatttgcag aatgtgcaat
     2881 ctcttcaaat tcaattttct cccattcatt ggtgtattca caataaacct caacatattc
     2941 ctttgtttcc ggattataga caaagtcaca tacataagaa tgggattcag aatgatgaac
     3001 agtattgagt tcaaggttat cagcggtaaa aagtccattg acaacagcgg ataatttcat
     3061 tattactgct ttcccttcca tgaacttgta agacgcagat attaccctgg tatcgtttgc
     3121 cacatcaatt tcgaatttaa atccttcaaa atccagatcg catgcaattg catttggtat
     3181 tccgtctgcg ttatagcttg caccgaatac aaaccggagc agtgtctggc ttccgatttt
     3241 cagatcggct tgtaatgaag caggaaaatc tcctgtatat tcttcatcaa atggattttc
     3301 cacgattaca ccttcataat tgtatattgt aaacgtggca ttattcattt ttgaatttcc
     3361 ggaagaaggg aactttataa tgaatttatc cccacccgga cttatatccc agtcgctggc
     3421 agaggaattc catgtatagg ttcccacgtt tctgtcccag aattcctgta tactttccgg
     3481 gtcttcgtca gcacagcctt ttatggactc gaatacatcg tttacctgca gggttcgtgc
     3541 ggcacatcca tgtatcgagc gaaatacgga atagagacct gttccggtaa aatccccgat
     3601 tccttttgat ttgcctgatc ctgttagttg tatcatatta ccgatagctt caactgtttg
     3661 agttgatctc atacggttaa gaaccccgat caggtcaatt ccggcagact caatagtagc
     3721 tttgttttct tcaacacttc ttgtggtgaa tgattctgtc ttttccttat cactgctgca
     3781 tgataacata gaataaacaa gcaatgcaat aaggactgat ttaaaaagat tcttcatttt
     3841 gataattgtt tgggatttgt attgcatagt actataattg ttttgcctga ctctgtttcc
     3901 ttttttcttc agcatcattt accagccacc ttcatgatac aatgcctcaa tttctgaatt
     3961 tgaaagtgcc ctgttgaaca tatacacatc atcaagatat ccctgataaa aaccgtaata
     4021 tgtagataac cactgatatg tgccaaaatt cagataaact ggctgattac aaaaaacatt
     4081 tccgggtcca taggctgagt ccattaattg cccgttaaca tataggtcaa ggctgactcc
     4141 attatacaca atagccacat gcgtccagtc actgcaatcg gtaaccgcat ggacatcaag
     4201 tccataacca tcggagctat cagtcagata gacagccgca tcaaccccat cctttgtttt
     4261 tcctattctg taggaatttc tgcttcccga tgaaattatc tgattaccaa tatcggtact
     4321 tttaatccag catgcaacgg taatatgccc ctgaactatc agtgaatttc caatatatat
     4381 tttgttgctg atgccattaa aatagtatgc tttctcagga tttccgaatc tgtctgcgac
     4441 aagggtggcg ccgtatacag tgccattatt attattaccc gattcatcat ttgcattgcc
     4501 gttaaaaggc cagtaagccg caagaccccc gattggcaaa tcttctacag tgctgaaggt
     4561 tatttcgttt ccgtaagtaa taccatcatc attaatcgag taagccctga cataatatgt
     4621 ggtattggat tccagtccgg ttagcagact tgtgaatgat cccgtgccgc catcgtttgt
     4681 tgttttgtta tccagtacct ccggagccgg tgaagtgttc cagcaaactc ctcttgaatt
     4741 aacaggcaaa acactgctga tgactgaaac tatttctgct ttatcagaca tgatatttct
     4801 tgcccaaagt gtggttacag agggttttgg aggagcaggc cctttaaggc atcttacatt
     4861 ggcgccattt gacttataac cccagctata accctctatg ctatattggt gttgcaggct
     4921 tctgtaaaat gcctgattgt caggatattc agttgaggtc caaaagctgg caaactcacc
     4981 tgtaacaaat aaagctccat tacgaaatcc tccggttggc aaagctgaaa atccactttc
     5041 attagtagca cctgtatttg gttcgctcca gtgactaaat ccttgttcct taagttttcc
     5101 gcccgcaatt tcatttcctc caagtgctct attcatcact gaccattctt catttgttgg
     5161 aaggtgccaa ccaaccggac atagattatt attggcagca taccagttat agataccacc
     5221 atatgtattc ttataacaga tattatcatt taaccaacaa agagcacctt ctgacaggct
     5281 ttgccacgtg tcattatctg ttataacagg tattgattca ccattgttat attttgtagt
     5341 ccttaaattt tcagccatcc attcctgatt accaatcttt atggttttat aaaagttacc
     5401 ttcaatatct gttatcactc cgtaagcgag gtttgaatta aaagcaacag gaggtatgta
     5461 atcacaggtt acaaatactt cttcattacc ataggctgtc cctgcactat tagttgcata
     5521 tgcacgtaca taatatgttg ttcctggcgt taatggacca atgtgatcaa taaacaaacc
     5581 ggttccggta ccactgtttg tcttgttatt tgttgttgtt ggattagttg acaaactcca
     5641 gcagatgcca cgggcaataa tttcatctcc tcc
//
LOCUS       DAOSNB010000165         5620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14561_length_5620_cov_4.246541, whole genome shotgun sequence.
ACCESSION   DAOSNB010000165 DAOSNB010000000
VERSION     DAOSNB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14561_length_5620_cov_4.246541"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /gene="argS"
                     /locus_tag="PLG42_07390"
     CDS             <1..1212
                     /gene="argS"
                     /locus_tag="PLG42_07390"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOU31069.1"
                     /translation="LVGKYYVLFENEYKKQVEALMKQGVDEETARQTAPIIIEAREML
                     VKWEAGDTKTVEIWKMMNSWVYQGFDETYRKLGVDFDKIYYESDTYKDGKELVLKALD
                     NKIFYKKEDGSIWADLTADGLDHKVLLRSDGTAVYITQDIGTAVVRHNDYKFDKCIYV
                     VGNEQNYHFQVLKAILKKMGFAWADGLIHYSYGMVELPEGKMKSREGTVVDADDLIEE
                     MINTARDVSLELGKLDGYSEEEKNEIFRKVGLGALKYFILKIDPKKNMLFNPSESIDF
                     DGNTGPFIQYTYARIRSVMRKAIQNKIDPDPELLQSAQPNAKEIELIKLIKKFSETVT
                     ESAVTYNPANIANYCYELAKEFNQYYHDYTILGEKDPVTRNFRLMLSDRIAFVLSEAM
                     GLLGIEMPERM"
     gene            1235..2554
                     /gene="ffh"
                     /locus_tag="PLG42_07395"
     CDS             1235..2554
                     /gene="ffh"
                     /locus_tag="PLG42_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOU31070.1"
                     /translation="MFENLTERLERSFKILKGQGRITEINVAETLKDVRRALLDADVN
                     FKIAKQFTDTVKQKALGQQVLTAVNPSQMMIKIVHDELAELMGSEKSDINLKANPAII
                     LIAGLQGSGKTTFSGKLAQWVKNKHNKKPLLVACDIYRPAAIEQLKIIGNQIQTPVYT
                     GQPGQNPVSIAIESVKEAKLKGYDVLILDTAGRLAIDEEMMAEIASIRDAIKPAEILF
                     VVDSMTGQDAVNTAREFNERLDFTGVVLTKLDGDTRGGAALSIKAEVNKPIKFVSTGE
                     KLDSIDIFYPKRMADRILGMGDIVTLVEKAQEQYNIEEARKLQKKLARDQFDFNDFMT
                     QIQQIKKMGNVKDLIGMIPGVGKAIKDLDIDDKAFKRIEAIIQSMTPEERSNPHIING
                     SRRKRIAAGSGTSVPEVNKLLRQFDEMRKMMKTIAKGGNPAKLINRR"
     gene            2566..3435
                     /locus_tag="PLG42_07400"
     CDS             2566..3435
                     /locus_tag="PLG42_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOU31071.1"
                     /translation="MQIIDGKKIAAEIKREIAAEVDKRKSRGLKIPHLAAILVGNDPS
                     SEIYVSNKIKDCKEVGFNSTLIRFDSNVQENELIDNIKKLNSDYEVDGFIVQLPLPDT
                     ISENKIIESIDPSKDVDGFHPVNVGKMVLGIPSFLPATPFGIIELIKRTGIKTAGKHC
                     VVVGRSNIVGRPVSILLSLKGIDATVTVVHSRTENIKDIILQADILIAALGSPGFVKA
                     DMVKEGVVIIDVGTTRVVAPETKSGWKLVGDVDFENVAPKCSYITPVPGGVGPMTRVS
                     LLRNTLLAASLKD"
     gene            complement(3441..4355)
                     /locus_tag="PLG42_07405"
     CDS             complement(3441..4355)
                     /locus_tag="PLG42_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31072.1"
                     /translation="MGIFPDSLYNLSGLNSEYDDYNLNLYSVSGTHPVIFSSNRGSKG
                     GQFDFVQGVISFDFDKETGNFVLSSDITSDIYYATIINKANTALNDFGPYSYFSSSDG
                     YEYMIYASETADNGLDLFYVKNLPRFGNNAPNVTGPFPITVINTSFNDSYLTFDFNGD
                     SIYFCSDRDGNYDIYVLKRNAGIVTDLFFSGAPELAVKVDSINSSFDDMAPYIYMNIM
                     VFASNRPGGLGGFDLYYSVYKNGKWSSPFNLGPSVNSSHNEFRPVIIYNKDFSNQAIL
                     FSSDRPGGSGRYDLYFTGFNLPVSRVIY"
     gene            4802..>5620
                     /locus_tag="PLG42_07410"
     CDS             4802..>5620
                     /locus_tag="PLG42_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU31073.1"
                     /translation="MEEERHWFSQEEDEVQKIIQRFERMKRNNENYFFDVIEFETIID
                     YYLERNNQARAFEAASIASKQHPNSVPILLRNAKVLLDRGRIAESLLILKKLETIEPG
                     NHEIFLVKGTALGMMGDIQGARKMFDLALAKDLDDVENILFSITSILQNLNYYKEVIP
                     YLERLIELEPYYPEHLYDMAYAYEKIDDFENSIKYYTRFLDEEPFSDSAWYNLGMIYN
                     KTERHYEALEAYDYALAINPQNDFALFNKGVVLAYLGKYEEAIHAYQEYLQIEPE"
BASE COUNT         1960 a          953 c         1142 g         1565 t
ORIGIN      
        1 cttgttggaa aatactacgt gttgtttgaa aatgaatata aaaagcaggt agaagctttg
       61 atgaaacagg gggttgacga agaaacagca aggcaaacag ctccaataat cattgaagcc
      121 agagagatgc ttgtaaaatg ggaagccggc gacacaaaaa ccgttgagat atggaaaatg
      181 atgaattcat gggtctatca ggggtttgat gagacatata gaaaattggg tgtcgacttt
      241 gataaaattt attatgaatc agatacttat aaagatggaa aagaacttgt tctgaaagct
      301 cttgataata aaatatttta caaaaaagaa gatggctcca tatgggctga ccttactgcc
      361 gacggactcg atcataaagt acttctcagg tcagacggaa cggcagttta tataacccag
      421 gatataggta ctgctgttgt aagacataat gactacaaat ttgataagtg catttatgtt
      481 gttggtaatg agcagaatta tcatttccag gtcttgaaag ctatattaaa gaaaatgggt
      541 tttgcatggg ccgatggctt aatacattat tcctatggca tggttgagct tccggaaggg
      601 aagatgaaat cacgcgaagg gactgttgtt gatgcagacg acctgattga agaaatgata
      661 aatacagcac gggatgtgtc gctggaactg ggtaaactgg atggttattc tgaagaagaa
      721 aagaatgaaa ttttcaggaa agttggactt ggagctttaa aatacttcat ccttaaaatt
      781 gacccaaaga aaaatatgct tttcaaccct tccgaatcaa ttgatttcga cggtaacaca
      841 ggtccattta ttcagtatac ttatgccaga attagatcgg tgatgcgaaa ggcaatacag
      901 aacaaaatag atcctgaccc cgaacttttg caatcagcac aaccaaatgc aaaagagata
      961 gaacttatta aactgattaa aaaattttca gaaaccgtca cagaatcagc agtgacttat
     1021 aatccggcta atattgcaaa ttactgttat gagctggcga aggaatttaa tcagtattat
     1081 cacgattata caattctggg ggagaaagat cctgtaacaa gaaacttcag actcatgttg
     1141 tccgacagaa tcgcttttgt tctttcggaa gcaatggggc ttctgggtat tgaaatgcct
     1201 gaaagaatgt agtacctttt ataatgaata taaaatgttt gagaatctga cagaacggct
     1261 tgagagatca tttaaaatcc ttaagggtca ggggagaata acagaaataa atgttgctga
     1321 gacccttaag gatgtaaggc gtgcattgct agatgcagat gtcaatttta aaattgcaaa
     1381 acagtttact gacacagtta agcagaaagc tcttggccaa caggttctga ccgccgtcaa
     1441 tccgtcgcag atgatgatta aaatcgtcca cgatgaactg gctgaactga tggggtcaga
     1501 aaaatctgac attaacctta aagcaaatcc tgcaattatt cttattgccg gtttacaggg
     1561 ttctggcaaa acaacattca gcgggaaact tgcccagtgg gttaaaaata aacataacaa
     1621 aaaacctttg cttgttgcct gtgacattta tcgtccggca gctatagagc agctaaaaat
     1681 aataggaaat cagattcaga cacctgttta tacaggacaa cctggccaaa accccgtatc
     1741 gatagccatc gaatcagtaa aggaggcaaa acttaaaggt tatgacgtac tgatactcga
     1801 tactgcagga agacttgcaa ttgacgaaga gatgatggct gaaatagcat cgatacgcga
     1861 tgcaatcaaa cctgctgaaa tattgttcgt ggtcgattca atgacaggcc aggatgctgt
     1921 gaacacagcc agggaattta atgaacgcct cgattttaca ggcgtggtac taacaaaact
     1981 ggatggcgac acaagaggtg gtgcagccct gtcaataaag gccgaagtca ataaaccgat
     2041 aaaattcgtc agcacaggag aaaagctcga ttctattgac atattttatc cgaaaagaat
     2101 ggctgacaga attctgggca tgggagacat tgtgacactg gttgaaaaag cccaggaaca
     2161 atacaatata gaggaggccc gtaaactaca gaagaagcta gccagagacc agtttgattt
     2221 taacgatttc atgactcaga ttcagcaaat taagaaaatg ggtaatgtca aggatctgat
     2281 tggcatgata ccgggtgtgg gaaaagcaat taaagatctc gacatagatg ataaagcttt
     2341 caaaagaatt gaagccatta tacagagcat gaccccggag gaaagaagca atccccatat
     2401 tattaacgga agccgacgca aaagaatagc tgcaggaagt ggtacttcag tacctgaagt
     2461 gaataaactt ttaagacaat ttgatgagat gcgaaaaatg atgaaaacca tagccaaagg
     2521 cggtaatccg gcaaaactta ttaataggag ataaactcat ccataatgca gataattgac
     2581 ggtaaaaaaa ttgcagctga aataaaaagg gaaatagcag ctgaagtgga taaaagaaaa
     2641 tcaagaggtc ttaaaattcc acatcttgcg gctattctcg ttggaaatga cccttcaagt
     2701 gaaatttacg tctcaaacaa gataaaagac tgtaaagagg taggattcaa ttcaactctt
     2761 ataagattcg actcaaatgt tcaggagaat gagttgattg ataatataaa aaaactcaac
     2821 agcgattatg aggttgatgg ttttattgta caacttcctc ttcctgacac tatttcagaa
     2881 aataagataa ttgaatccat agatccctca aaggatgtag atggttttca tccggttaat
     2941 gttggaaaaa tggttctggg tatcccctca ttcctccctg ctactccttt cgggataatt
     3001 gaactgataa aaagaacagg aataaaaact gcaggaaaac attgcgttgt agtcggaaga
     3061 agtaatattg ttggccggcc agtaagtatt ctactttctc tgaaagggat agatgccacc
     3121 gttactgtag tacatagtag aacagaaaac attaaagata ttattttaca ggccgacata
     3181 cttatagcag cactaggatc ccccggattc gttaaagcag atatggtaaa agagggcgtt
     3241 gtaattattg atgtcggaac caccagagta gtagcacctg aaacaaaatc gggctggaaa
     3301 cttgttggtg atgttgattt tgaaaatgtt gctccaaagt gctcatatat tactccagtt
     3361 ccaggtggag ttggtcctat gactagagta tcacttttaa gaaataccct tctggctgca
     3421 agtctgaaag attaaagaaa tcaataaata acccttgata cgggtaggtt gaaacctgtg
     3481 aaataaaggt catatcttcc gcttccaccc ggtctgtcgg atgaaaataa tatcgcctga
     3541 ttagaaaaat ctttattata aattattact ggtctgaatt cattgtgtga tgaattaaca
     3601 gatggcccaa gattgaaagg agaactccat ttaccgtttt tataaactga ataatataaa
     3661 tcaaacccgc ctaaccctcc cggtcggttc gatgcaaaaa ccatgatatt catataaata
     3721 tatggagcca tgtcgtcaaa actgctgttg atactgtcaa cttttactgc cagttcggga
     3781 gccccagaaa aaaatagatc ggtaacaatc ccggcgtttc ttttcaaaac atatatatcg
     3841 taattcccgt ctctgtcaga acaaaaataa attgagtcgc cgttaaaatc aaaagttaaa
     3901 taactgtcgt tgaatgaagt gtttattacc gtgatcggga aaggccctgt aacatttggt
     3961 gcattattcc caaaacgtgg caggtttttg acataaaaca gatcaaggcc gttgtctgct
     4021 gtttcagatg cataaatcat atattcatag ccgtcactcg aactgaaata gctgtaaggc
     4081 ccaaaatcat tgagagctgt attagcttta tttatgatag tcgcataata tatatcggac
     4141 gtaatatcgc tgctaagcac aaaattacct gtctctttat cgaaatcaaa cgaaattacc
     4201 ccctgaacaa aatcaaattg ccctcccttg cttcccctgt tgcttgaaaa aataaccgga
     4261 tgtgtacccg aaactgaata cagatttagg ttgtaatcat catattccga attcagccct
     4321 gagaggttat atagcgaatc aggaaaaatt cccatgggaa actttactgg attatccttt
     4381 tttttgcatc cactgctata aaccatcaag aaaaatgaaa aagcagtgaa aatgaataaa
     4441 taattaggtt tataaaagat tttcatatta aaaaatatgt ttcaacaaaa ttaagatttt
     4501 attttcttat cgtgagagaa accccctcaa ataattgaag aaccgggttt ggcccggccc
     4561 ttctgcatag aatttaggtt aattctaggg tatattgggt atgagggttt ccaaatatat
     4621 agatgatttt tattaaccaa cggttgcgtt gtgttattaa taaatagttt tttgtttctt
     4681 tttgtatttt attaacagat tttaatgttt attaaagtgt taaaatgttt ataaatgata
     4741 tttgcttaac aatttgacga aaaaaataat attaactttg aaaagtttta ctgaagatta
     4801 aatggaagaa gaaagacact ggttttcaca ggaggaagat gaagtccaga aaatcattca
     4861 gagatttgaa aggatgaaaa gaaataatga aaattatttc tttgatgtta ttgaatttga
     4921 gacaattatt gattattatc ttgaacggaa caatcaggca agggcatttg aagcagcttc
     4981 aatagcaagt aagcaacatc ctaattcagt acctattctt ttaagaaatg caaaagtatt
     5041 gcttgatagg ggaagaatag ctgaatccct gctcattctt aaaaaacttg agactataga
     5101 acctgggaat catgaaattt ttcttgtaaa aggcactgct cttggtatga tgggcgacat
     5161 tcagggtgca agaaaaatgt ttgaccttgc ccttgcaaaa gatttggatg atgtggagaa
     5221 tatacttttt tcaataacct caatactgca aaaccttaat tactataagg aagtaatacc
     5281 ttatcttgaa cgacttattg aacttgaacc ttactacccc gagcatctgt atgacatggc
     5341 atatgcctac gaaaaaatcg atgattttga aaacagtatt aaatattaca ccagatttct
     5401 cgatgaagaa cccttctcag atagtgcctg gtataacctt ggaatgatct ataataaaac
     5461 ggaacgtcat tacgaagccc tcgaagcata cgattatgca cttgctataa atcctcaaaa
     5521 tgatttcgca ctatttaata agggtgtagt cctggcttat cttggcaaat atgaagaggc
     5581 aattcatgca tatcaggaat accttcagat tgaacctgaa
//
LOCUS       DAOSNB010000166         5592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14638_length_5592_cov_4.953224, whole genome shotgun sequence.
ACCESSION   DAOSNB010000166 DAOSNB010000000
VERSION     DAOSNB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14638_length_5592_cov_4.953224"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1580)
                     /gene="nadB"
                     /locus_tag="PLG42_07415"
     CDS             complement(3..1580)
                     /gene="nadB"
                     /locus_tag="PLG42_07415"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOU31074.1"
                     /translation="MKKRTDFLVIGSGIAGLMFALKASRYGKVAIVTKSSIEESNTKY
                     AQGGIAAVLSETDDFEKHVRDTLIAGDGFCNEEVVRMVVREAPERIKDMIEYGVKFDK
                     KEDGSFDLAKEGGHSEHRILHHRDNTGEAIQKALVARVKANPNIEVYENHFAIDILTQ
                     HQLGEEVTRERPDIKCFGAYVADLVNPRVITFLSRITVMATGGVGNVYLTTTNPEIAT
                     GDGIAMVYRAKGVIENMEFIQFHPTSLYDPGRRPSFLITEALRGYGAVLRNMQGEKFM
                     HRYDSRGSLAPRDIVARAIDNEMKIWGDDHVWLDCTHLDPAGLKKNFPNIYRHCLERG
                     IDITRDMIPVVPAAHYCCGGIKVDIHGQSTIDRLYAIGECSCTGLHGANRLASNSLIE
                     GAVYAHRAAEHAGHRVGSIDFEEKIPDWDYKGTTHLEEMVLITQSTRELQSIMSNYVG
                     IVRSDLRLERALRRLEIIYQETESLYKKSLISKKICELRNLINVGYLIIKMAKNRRES
                     IGLHYSIDYPRHSPSEF"
     gene            1792..3342
                     /locus_tag="PLG42_07420"
     CDS             1792..3342
                     /locus_tag="PLG42_07420"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOU31075.1"
                     /translation="MALEDLFKKLVSHCKEYGYVFPSSEIYDGLSAVYDYGQYGVELK
                     NNIRKYWWDSMVLLNRNIVGIDAAIFMHPTTWKASGHVDAFNDPLIDNRDSKKRYRAD
                     TLIEEHIEKIEEKVQKEIDKARAKFGDAFDEAKFRSTNQRVLSLQAKIDEIHARYSRA
                     MKENNLAELRQIIIDNEIVCPISGTRNWTEVRQFNLMFSTEMGSTADGSIKVYLRPET
                     AQGIFVNFLNVQKSGRMKVPFGIAQVGKAFRNEIVARQFLFRMREFEQMEMQYFVTPG
                     TELEWYEYWKEKRMKWHLALGFGKENYRFHDHEKLAHYANAACDIEYKFPFGFKEVEG
                     IHSRTDFDLKQHQIYSGKKMQYFDPDKNESYIPYVIETSIGLDRMFLHIISAAYHEEE
                     IEKEDGTKDMRVVLRLPSCLAPVKLAVLPLIRKDGLPEIAEKIVEDLKFDFNCQYDDK
                     DSIGRRYRRQDAIGTPYCITVDHQTLEDQTVTIRYRDSMEQERVPVAGLREIIAEKVS
                     IRSLLKQL"
     gene            3344..4609
                     /locus_tag="PLG42_07425"
     CDS             3344..4609
                     /locus_tag="PLG42_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU31076.1"
                     /translation="MNRVLIITYYWPPGAGAGVQRWLKFSKYLPLHGWEPVILTVDPA
                     FAQYPSLDESLLHDIPENIKIVRTPARNYFRFAGRDRSQIPSAGFASEGRKSFLSYLM
                     RFARGNFFIPDPRRGWNPYAFREACKLIEKENITRVITSSPPHSTQLIGLKLKKKYPH
                     IKWIADLRDPWTDIYYYKEFFHTYPARLLDRHYEKSVLRKADKIITVGDRLKIMLASK
                     VSTAEAKTYVVTNGYDDEDFRNLKPSEPEIFTISYTGTLSPAYPLKGFAEAVKKLTDS
                     GRKIRVRFVGTISSTQKKTIYENVPEEFLDFIPYVSHTEAVKHMISSSALLLIIPDHP
                     GNRCILTGKLFEYIASGKPVLCLGPADGDAALVLEMTGAGITTGYKETNTIARIISDF
                     MEKRFIPSKEKTEIFSRYNLTRKIAEIIK"
     gene            4676..>5592
                     /locus_tag="PLG42_07430"
     CDS             4676..>5592
                     /locus_tag="PLG42_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOU31077.1"
                     /translation="MGIVFRQSVKGTIFVYTGLILGFITTGILFPRIYSPEQIGLLKI
                     IVAYSSLISMVGTLGINGAIIRLFPWFRNDEKKHYGFLSLVLLVGFAGFLLNTSLLLI
                     FKPALLSSALQKSPLVAKYFNYLIILVFFQIFFTILDNYYTALYNSVHGTFLREVFQR
                     VLIIIFIGLFFFDILNFDQFVILYVAALCLPTLYITLTLIKENQFSLSTDFKHIDRQM
                     AFSIASVSLFSILNGFSLLVIQNIDVIMINSMVGLDFAGIYSICFFFGVVISMPSRSL
                     SRIANVLSADAWKNNDLVTIREIYYKSCL"
BASE COUNT         1688 a         1236 c         1034 g         1634 t
ORIGIN      
        1 ttttagaatt cagatggtga gtgccgcgga taatcaattg aataatgaag gcctatactt
       61 tctcttctgt tttttgccat ttttattatt agatacccga cattaataag gtttctcagt
      121 tcgcatatct tttttgatat aagcgatttt ttgtacaggc tttctgtttc ctgatatata
      181 atctcaagtc ttctcaatgc tctttcgagc cgaagatctg atcttacaat tcccacataa
      241 ttgctcatta tactctggag ttctctggtg ctctgtgtaa tcagaaccat ttcttccaga
      301 tgtgttgttc ccttgtaatc ccagtcgggt attttttcct caaaatcaat acttccaact
      361 ctatggcctg catgctcagc agcacgatgt gcatagactg ctccttcaat aagtgaattg
      421 gaggcaagcc ggtttgctcc atgaagccct gtacatgagc attctccaat tgcataaagc
      481 ctgtcaatag tgctctgccc gtggatatca actttaatgc cgccgcaaca gtaatgtgct
      541 gcaggaacta caggaatcat atcccttgtg atgtctattc ccctttcaag gcaatgcctg
      601 taaatattgg gaaagttttt tttcagacct gccgggtcga gatgggtgca atcgagccat
      661 acatgatcat caccccatat tttcatttca ttatctattg cccttgccac tatgtccctc
      721 ggagccaggg aacccctgct gtcgtatcgg tgcatgaatt tctcaccctg catatttcta
      781 agaactgctc cgtaacctct caaagcttca gtaataagaa aagagggtct tctgccaggg
      841 tcataaaggg atgtgggatg aaactggata aactccatat tttcaataac acctttggct
      901 ctgtaaacca tcgcaatacc gtctcctgta gcaatctcag gatttgtggt agtaaggtaa
      961 acatttccga ccccaccggt agccatcaca gtaatccttg aaagaaaagt tattacccta
     1021 ggatttacaa gatccgcaac ataagctcca aaacacttta tatcaggcct ctctcttgta
     1081 acctcttcac caagctggtg ttgtgttaag atgtcaattg caaagtgatt ttcatacact
     1141 tcaatattcg gattggcttt cacccttgcc acaagtgcct tctgaattgc ttcacctgta
     1201 ttatccctgt gatgaaggat tctgtgttct gaatgccctc cttccttggc cagatcgaac
     1261 gaaccatctt ccttcttatc aaatttaact ccgtactcaa tcatgtcttt aatcctttca
     1321 ggagcctccc taaccaccat ccgcacaacc tcttcattgc aaaatccgtc acccgctata
     1381 agcgtatctc gtacatgctt ttcaaaatca tcagtttcac tcagtacagc ggctatacca
     1441 ccctgtgcat attttgtgtt cgactcttca atggaagact ttgtaacaat agcaacctta
     1501 ccatatctgg aagctttgag agcaaacatt aaaccggcaa ttcctgatcc aataactaga
     1561 aaatccgttc tcttcttcat gatacctggt ttggaaacgt aaagatataa atagatttta
     1621 tcagctctcc caaaaaattt gataaatctt ctatcaacct gctatcccta tgaaaaactt
     1681 tttccttatt ataattttag aacacgctaa attgctgttt caaacctgat ttgaaaaatt
     1741 aagattttga attacttttg ccttcttgtt taataattat tacagattat tatggctctt
     1801 gaggatctct ttaagaaact tgtatcacat tgcaaggaat atggttatgt atttccttca
     1861 agtgaaattt atgatggctt aagtgcagtt tacgactacg gtcaatatgg tgtggaacta
     1921 aaaaacaaca taagaaaata ctggtgggac agtatggtat tgctgaacag aaatattgta
     1981 ggcatagatg ctgctatctt tatgcatccc actacctgga aagcttccgg acatgttgat
     2041 gcattcaacg atcctcttat cgacaacagg gattcaaaaa agagataccg ggcagatacc
     2101 ctcattgaag aacatattga aaagatagaa gaaaaagtac agaaagaaat agataaagcc
     2161 cgtgcaaaat tcggtgatgc tttcgatgag gcgaaattca gaagtacaaa tcaacgtgta
     2221 ctgtccttac aggcaaagat tgatgagatt cacgcgcgtt actcacgggc aatgaaagaa
     2281 aacaatcttg cagaattacg ccagataatc atcgacaatg aaatagtttg ccctatttct
     2341 ggaacaagga actggacaga agtcagacag ttcaatctga tgttctccac tgaaatgggg
     2401 tcaactgccg atggttcaat aaaagtatac ctccgaccgg agacagcaca gggaatcttt
     2461 gtgaatttcc ttaatgttca gaagagcggc cgaatgaaag tgcccttcgg aattgcacag
     2521 gtggggaaag cttttcgtaa tgagatagtt gctcgtcagt ttctgttccg tatgcgggaa
     2581 tttgagcaga tggagatgca gtatttcgtt actccaggca ccgagcttga atggtacgaa
     2641 tactggaagg aaaaacgtat gaaatggcac ctcgcacttg gctttggtaa agaaaattac
     2701 cgttttcacg accatgaaaa gctggctcac tatgcaaacg ctgcttgcga cattgaatac
     2761 aaatttcctt tcggcttcaa agaagttgaa ggcatacact ccagaaccga ttttgacctg
     2821 aaacagcacc agatatacag tggtaagaaa atgcaatatt tcgaccctga taaaaacgaa
     2881 tcttatatcc cttatgtcat cgaaacatcc atcggtctcg acagaatgtt tctacacata
     2941 atatccgctg catatcatga ggaagagatt gaaaaagaag atggcacaaa agatatgcgg
     3001 gttgtactcc ggctcccatc ctgccttgca cctgttaaac ttgctgtttt gcctcttatc
     3061 agaaaagacg gactccctga aattgcagaa aaaattgttg aagaccttaa gtttgacttt
     3121 aattgtcagt acgacgacaa agattcaata ggaagaagat accgtaggca ggatgcaatt
     3181 ggaacaccct attgtattac tgttgaccat cagactctgg aagaccagac agtaacaatc
     3241 agataccgtg attcaatgga acaggaaagg gttcctgtgg cagggctcag ggaaataatt
     3301 gcagaaaaag ttagcatcag aagcctgctt aaacagctat aagatgaacc gcgttcttat
     3361 aattacctac tactggcccc caggtgcagg cgccggggta cagagatggc ttaaattttc
     3421 caaatacctg cccctgcacg gatgggaacc tgttatactg acagtcgacc cagcatttgc
     3481 acaatatcct tctttggatg aatcactgct acatgatatc cctgaaaaca ttaaaatagt
     3541 acgaaccccg gcacgcaatt atttcagatt tgcaggcagg gacaggtcac agatcccctc
     3601 ggcaggattt gccagcgaag gacgtaaaag ctttctatca tatttaatga gattcgcaag
     3661 aggcaacttt ttcattcccg atccacgccg cggatggaac ccttatgcct tcagggaagc
     3721 atgtaaactt atagaaaaag aaaatataac cagagtcatc accagcagcc cccctcactc
     3781 cacacagctt attggactta aactcaaaaa aaaatatcct catataaaat ggattgccga
     3841 cctgcgtgac ccatggacag atatttatta ctacaaggaa ttctttcata cttatccggc
     3901 cagacttctc gacaggcact acgaaaaaag cgtactcaga aaagccgata aaataattac
     3961 agtaggcgac aggcttaaaa ttatgcttgc ttcaaaagta agtactgcag aagcaaaaac
     4021 atatgtggtc accaacggtt atgacgatga agattttagg aacctgaaac catctgaacc
     4081 ggaaatattt accatttcat atactggcac tctttcgcct gcctaccctc ttaaagggtt
     4141 tgcagaggca gtgaaaaaac tcacagattc aggcagaaag attcgcgtca gatttgtagg
     4201 aacaatctca tcaacgcaga aaaaaaccat atacgaaaat gttcctgaag aatttttaga
     4261 tttcattccc tatgttagcc atactgaagc tgtcaaacac atgatctcct catctgccct
     4321 tttattgata atacccgacc accctggaaa caggtgcatt cttacaggaa aacttttcga
     4381 gtatatcgcc tcaggtaaac ctgtactctg cctgggccct gccgacggcg acgcagctct
     4441 ggtactggaa atgacaggcg ctggaattac aaccggatac aaagaaacaa atacaatcgc
     4501 ccgcattatt tcagatttta tggagaaaag attcatacct tcaaaagaaa aaacagaaat
     4561 cttttcacgg tataacctca cccgtaaaat cgcagaaata ataaaataaa ctaccctttt
     4621 ctgagttaat tgcctccttt ttgtaaattt gctcataacc taaaattact taccgttggg
     4681 aattgtattc agacaatccg taaaaggtac aatatttgtt tacaccgggc tcatcctcgg
     4741 atttattaca accggaattc tttttcctcg aatctattca cccgaacaga tcggattgct
     4801 aaaaattata gttgcctatt cctcgctgat ttccatggtt ggaacattgg gcatcaatgg
     4861 tgcaataatc aggcttttcc cctggttcag aaatgatgaa aaaaaacatt atggatttct
     4921 ttccctggta ctgctggtag gctttgcagg attcctgctt aacacctccc ttcttttgat
     4981 ttttaaacct gcacttctaa gttcagccct tcagaaatca ccactcgtag caaaatattt
     5041 caactacctc attatactgg ttttcttcca gatattcttc acaattctcg acaactatta
     5101 tactgccctt tataacagcg tgcatggaac atttcttcgt gaagtttttc agcgagtgct
     5161 gataataata tttatcgggt tattcttttt tgacattctt aactttgatc agttcgtaat
     5221 tctttatgtt gcagcactct gccttcctac actttatata actcttaccc tgataaaaga
     5281 aaaccagttt tcactgtcca ccgactttaa acatatcgac cggcaaatgg ctttttcaat
     5341 agcttctgtg tcattattca gtatattgaa tggattctcg ctacttgtta ttcagaatat
     5401 cgacgtaatt atgataaaca gcatggttgg gcttgacttt gccggtatct actcaatatg
     5461 cttctttttc ggagttgtaa taagcatgcc ttcaagatcc ctcagcagaa tagcaaacgt
     5521 cctttcggct gatgcatgga aaaacaatga tctggtaaca ataagggaga tatactataa
     5581 aagctgtctg aa
//
LOCUS       DAOSNB010000167         5521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14855_length_5521_cov_4.711306, whole genome shotgun sequence.
ACCESSION   DAOSNB010000167 DAOSNB010000000
VERSION     DAOSNB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14855_length_5521_cov_4.711306"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="PLG42_07435"
     CDS             complement(<1..1426)
                     /locus_tag="PLG42_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HOU31078.1"
                     /translation="MRVEIMDTTLRDGEQMQGVSYSPLEKLHIATLLLKEVKVDRIEI
                     ASARVSKGEFEAACKISEWAGKNGFLDRVEVLGFVDGDVSLEWIRKAGLKVINLLCKG
                     SLRHLEKQLRKTPEQHVSDIKYVIRKASETGVDVNIYFEDWSNGMIHSQDYVHFMMES
                     LKEEPVKRFMLPDTLGILNPEQTYQFCSVMTEKYPDVHFDFHAHNDYDFAVANVFSAV
                     KAGIKGIHTTVNGLGERAGNVPLSSVVAVLHDHLGYKTGIDESQITKISQVVESYSGI
                     RIAVNQPVIGENVFTQTSGIHADGDSKANLYFNNLLPERFGRKRKYALGKLSGKATIK
                     KNLEEFGLNLSPESIERVTQRVIELGDKKECVTTEDLPFIISDVLGAEKIPYRVFIKN
                     YSLALSYGMRPVASLLVEIDGQVYEETSSGDGQYDAFMKALRKIYDRLGKELPVLTDY
                     KVSIPPGGKTDALVETVISWNFKGM"
     gene            complement(1423..2022)
                     /gene="leuD"
                     /locus_tag="PLG42_07440"
     CDS             complement(1423..2022)
                     /gene="leuD"
                     /locus_tag="PLG42_07440"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOU31079.1"
                     /translation="MPGVKFTVLESRCVPLPVENIDTDQIIPARYLTTTSRKGLGKHL
                     FHDWRYDKNGEPVKEFVLNNPVYSGSILVAGKNFGCGSSREHAVWALYDYGFRAVISS
                     FFADIFKNNALNNGLLPVVIPEENLKNLFMLIQQHPDMKVIIDLGHQLLEIPKTGKAI
                     RFDINPWKKECLLNGMDDIDYLLTMKEKIEEFEKKHIIP"
     gene            complement(2039..3433)
                     /gene="leuC"
                     /locus_tag="PLG42_07445"
     CDS             complement(2039..3433)
                     /gene="leuC"
                     /locus_tag="PLG42_07445"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOU31080.1"
                     /translation="MVKKTLFDKIWDAHLVQSIDGGPDVLYIDMHYIHEVTSPQAFNG
                     LRERGIGVFRPERTLATADHNIPTKDQHLPIKDQTSRKQVDTLAENCRAFGIEFFGMQ
                     HPNNGIVHIIGPELGYTRPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEMVLASQCIL
                     QYRPRRMRITVNGKTGKAVTAKDIILYIISRLSSGGATGYFVEYAGEAIENLSMEGRM
                     TICNMSIEMGARGGLIAPDDVTFRYLEDIEKSGGRKITTAEREFWKTLHTDTGARFDR
                     EVKFDAADIRPMITYGTNPGIAMPVDGTIPEPGNDENIDSYLKSLSYMGFKPGMKLEG
                     HKIDYVFLGSCTNGRIEDFRAFASVIKGRKKSPEVTALIVPGSKRVEKQIYDEGLDSI
                     FTEAGSEIRQPGCSSCLAMNEDKIPAGKYAVSTSNRNFEGRQGPGSRTILASPLTAAA
                     CAITGKITNPEEFL"
     gene            complement(3437..4930)
                     /locus_tag="PLG42_07450"
     CDS             complement(3437..4930)
                     /locus_tag="PLG42_07450"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOU31081.1"
                     /translation="MEEKVIVFDTTLRDGEQVPGCQLNTIEKIQVAQALDELGVDVIE
                     AGFPISSPGDFNSVVEISKVVTRPVICALTRAVKKDIEVAAEALQYAQRKRIHTGIGV
                     SPYHIEYKIRTTPDEIIRRAYDAVKYARKFVDDVEFYAEDAGRADNEYLAKVIEAVIE
                     AGATVVNIPDTTGYCLPEEYGEKIRYLKNNVPNIDKVIISTHCHNDLGMATANTIMGV
                     LNGARQVEVTMNGVGERAGNTSLEEVVMILKCHKHLNLYTDVNTQLIYKTSRLVSTLM
                     NMPVQPNKAIVGRNAFAHSSGIHQDGVLKKRENYEIINPVEVGVRESSIILTARSGRA
                     ALNHKLQQLGYKFEKEELDEIYYRFLKLADKKKVINDEDIKILTGEVFQGEKPLKLEV
                     LQVTCGKSTIPVATVGLKINNEVHTSTATGNGPIDAAFTAVKQLINKTVHLEEFLIQA
                     ITRGSDDIGKVHIQIEHEGKIYYGFSANTDIVTASVEAFVDAIAKIK"
     gene            complement(4942..>5521)
                     /locus_tag="PLG42_07455"
     CDS             complement(4942..>5521)
                     /locus_tag="PLG42_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOU31082.1"
                     /translation="RRNFLAGAGINSSYSVYQDYTGRATERVLALGIAIGSGYLFPTT
                     FEKEVYSDLTGERGVLMGALAGIMHAQYQVLRENGHSPSEAFNETVEELTQSLIRLVD
                     ERGMDWMFANCSATAQRGALDWMPKFRDAVLPLFKELYERVKTGKETERVLQALSNPN
                     YRDQLNAELNQLGSSEMWRAGAAVRKLRPGNK"
BASE COUNT         1292 a         1316 c         1160 g         1753 t
ORIGIN      
        1 ccataccttt aaaattccat gaaataacag tttccacaag ggcatctgtt tttccgccgg
       61 gaggtatcga cactttgtag tcggtaagaa caggcagttc tttgccgagg cggtcgtaaa
      121 tctttctcag agctttcatg aatgcatcat actggccgtc gcctgaagag gtttcttcat
      181 atacctggcc gtcaatctca accagtaggc ttgccacagg tctcatgcca taagataggg
      241 caagggagta gttttttatg aaaacgcggt aaggtatctt ttcagcacca aggacgtcgg
      301 agataataaa cggcagatct tccgttgtga cgcactcttt tttgtcgccc agctcaatta
      361 ctctttgtgt aaccctttca atggactcag gagagagatt gagtccgaat tcctcaaggt
      421 tcttttttat tgtggctttc cccgaaagct taccgagggc gtattttctt tttcttccaa
      481 aacgttcggg aagcaggttg ttgaaataca ggtttgcttt gctatcgcca tcggcatgga
      541 ttccagaagt ctgagtaaag acgttttctc cgataaccgg ctggtttacg gcaatcctta
      601 tcccgctgta ggattcgaca acctggctta tttttgttat ctgcgactca tcaatgccgg
      661 tcttgtatcc gagatggtcg tgtaacactg ccacaacgct cgataatgga acatttccag
      721 cacgttcacc cagtccgtta actgttgtat ggatgccttt tatgcctgcc tttactgctg
      781 aaaatacatt tgccacggca aagtcataat cgttgtgtgc atgaaaatca aaatgtacat
      841 ccggatattt ttctgtcatc accgagcaga actgatatgt ctgctcaggg tttagaatgc
      901 caagagtatc aggcagcatg aatcttttga caggttcttc tttgagcgat tccatcataa
      961 aatgaacata atcctgtgaa tgtatcatcc cgttcgacca gtcttcgaag tagatgttaa
     1021 catcaacgcc tgtttcactg gctttacgga taacatattt tatatcagac acatgctgtt
     1081 caggggtttt tcttaattgt ttttccagat ggcgcagtga gcctttgcaa agcaggttga
     1141 ttactttgag gcctgccttt ctgatccatt caagcgaaac gtcaccatcg acaaaaccaa
     1201 gtacttcaac cctgtcgaga aacccgttct ttccggccca ctcggagatt ttgcatgcag
     1261 cttcgaattc tcctttcgaa acccgtgctg aggcaatctc aatgcggtcg acctttactt
     1321 ctttcagaag aagtgtagct atatgcagct tctccagcgg agaatatgat acaccctgca
     1381 tctgttcacc atcgcggagg gtcgtatcca ttatttctac tctcatggga taatatgttt
     1441 tttttcaaat tcttctattt tttctttcat agtaagcaga taatcaatat cgtccattcc
     1501 attaagcagg cattcctttt tccatggatt gatatcgaac cttattgcct tacctgtttt
     1561 tggtatttca agcaactggt gcccgagatc tattattacc ttcatatcag gatgctgctg
     1621 tatcagcata aagagattct tcagattttc ttcaggaatc actacgggca gtagtccgtt
     1681 attcagggca ttgtttttaa agatatctgc aaaaaagcta gatatcaccg ccctgaatcc
     1741 gtaatcgtaa agagcccata cggcgtgttc cctgctcgag ccgcaaccga agtttttgcc
     1801 tgctacaagt attgaacctg aataaacagg attattcagt acaaactctt tcaccggttc
     1861 cccgttttta tcatatctcc agtcgtgaaa aagatgtttt ccgagtccct tgcgggatgt
     1921 tgttgtaaga tatcttgcag gaattatctg gtcagtgtcg atattttcga caggcaatgg
     1981 tacacatctc gactcgagta ctgtgaattt tacccctggc atataattaa atatttagtt
     2041 ataagaactc ttctggattt gtaatttttc cggttattgc acatgctgct gcagtaagag
     2101 ggcttgcaag gattgtccgg gatcctggtc cctgccggcc ttcgaagttt ctgtttgacg
     2161 tagaaacggc atacttccct gcagggattt tgtcttcatt catggcaagg cacgatgaac
     2221 atcccggctg gcggatctcg gaaccggctt cagtgaatat actgtcgagg ccttcatcat
     2281 atatttgttt ttccactctc tttgagcccg gtactatcag agcagtgact tccggagatt
     2341 tcttccttcc ttttattacc gatgcaaatg ctctgaagtc ctcgatacgt ccgttggtac
     2401 agcttcccag aaagacgtag tcaattttat gtccttcaag tttcatacct ggtttgaagc
     2461 ccatataact cagtgacttc aagtatgaat caatgttttc atcattaccg ggttccggaa
     2521 tagttccgtc taccggcatt gctattccag gattggtgcc ataagtaatc atgggcctga
     2581 tatcagctgc atcaaatttc acctccctgt caaacctggc acctgtatcg gtatgcaatg
     2641 ttttccagaa ttctctttca gctgttgtaa tttttcttcc accagatttt tctatatctt
     2701 cgagatatct gaatgtcaca tcatcggggg caataagtcc accgcgggca cccatttcta
     2761 tgctcatatt gcatatcgtc attctccctt ccatgctcag gttttcgatt gcttcaccgg
     2821 cgtattctac aaaataaccc gttgcgcctc ccgaagaaag ccgcgatatg atatagagta
     2881 taatatcctt tgctgtaacg gcttttcctg tttttccgtt tacggttatt ctcatccggc
     2941 gggggcggta ttgaagaatg cactgggaag caagtaccat ctcaacttcc gaagtgccaa
     3001 taccgaaggc tatcgcacca aaggcaccat gggtagaggt atggctatca ccgcatacta
     3061 ttgtcatccc cgggcgggta tatccgagct ccggtccgat aatatgcact ataccgttgt
     3121 tcggatgctg catgccgaaa aactcgatac caaaagcacg acagttttct gccagcgtat
     3181 cgacctgctt ccttgaggtc tgatccttta tcggtagatg ctgatccttt gtgggtatgt
     3241 tgtgatcggc ggtggcaaga gttctttcag gtctgaaaac accaatccct ctttccctca
     3301 aaccattgaa tgcctgagga ctcgtgactt catgaatata atgcatatcg atatagagaa
     3361 cgtctggtcc tccgtcaatc gactgcacaa gatgtgcatc ccatattttg tcaaaaagag
     3421 tcttttttac cactgcttat ttaatttttg caattgcatc aacgaatgcc tcaactgaag
     3481 cagtaactat atcggtgtta gctgagaatc cgtaatatat tttaccttca tgttcaatct
     3541 gtatatgcac ctttccgata tcatcgcttc cccgtgtgat agcctgaatg agaaactcct
     3601 cgagatgcac agttttgtta atgagttgct tgacagcggt gaaagcagca tcaatagggc
     3661 cattgcctgt tgctgttgaa gtgtgaactt cgttgtttat cttcagtcct actgtggcaa
     3721 ccggtatggt cgatttaccg catgtaacct gcaatacctc gagtttcaga ggcttttctc
     3781 cctggaatac ttcacctgtg aggatcttta tatcctcatc gttgataact tttttcttgt
     3841 cggcaagttt caggaagcgg taataaattt cgtcgagttc ttctttttcg aatttatatc
     3901 cgagttgctg gagcttatgg ttaagtgctg ccctgccgct gcgggctgta agaatgatcg
     3961 atgattccct tactccaact tctaccggat ttatgatctc gtagttttct cttttcttca
     4021 gaacgccgtc ctgatgtatg cccgatgaat gggcaaaagc atttctgccc actattgcct
     4081 tgtttggctg aacgggcatg ttcataagtg ttgagaccag gcggctggtt ttgtaaatca
     4141 gctgtgtgtt gacatctgtg taaaggttga ggtgtttatg acattttagt atcataacca
     4201 cttcttccag ggaagtgtta cctgctcttt cacccacacc attcattgtc acctccacct
     4261 ggcgggcacc gttgagtact cccatgattg tgttggcagt tgccatgcca aggtcgttgt
     4321 ggcagtgtgt tgaaataatc actttatcaa tgtttggtac attgtttttc aggtatctga
     4381 tcttttcccc gtactcttca ggcaggcaat agcctgtggt atcaggtatg ttaacaacag
     4441 tggcacctgc ttctattact gcttcgataa cttttgcaag atattcgtta tcggctcttc
     4501 ctgcatcttc agcatagaat tcgacgtcat caacaaattt tcgggcatac tttacggcat
     4561 cgtatgcacg ccgtatgatt tcatcaggtg ttgtacgtat cttatattca atatggtacg
     4621 gcgagactcc aatgcctgtg tgtatcctct tgcgttgtgc gtactgtaat gcttcagctg
     4681 ccacttcgat gtctttttta actgccctgg tcagagcaca tattacaggg cgtgtgacaa
     4741 ccttggaaat ttcaaccact gaattgaagt cgcccggact tgaaatcggg aatccggctt
     4801 caattacgtc cacgccgagt tcgtctagag cctgagccac ctgaatcttt tcaatggtat
     4861 tcagctggca tcctggcacc tgctcgccgt ctctcagcgt ggtatcgaat acaattactt
     4921 tttcttccat tactatttta cttatttatt accaggtctt aattttctta ctgctgcacc
     4981 agccctccac atttcggagc ttccaagctg gtttagttct gcgttgagct ggtccctgta
     5041 gttggggttg cttagagcct gcagtactct ttctgtttct ttgcctgtct taactctttc
     5101 gtacagttct ttgaagagtg gtaatacggc atccctgaat tttggcatcc agtcgagagc
     5161 cccgcgctgg gctgttgcac tacagttggc aaacatccaa tccatccccc tctcatcaac
     5221 cagccttatc aaactctgcg tcagctcttc aaccgtctca ttaaaggctt cggaaggtga
     5281 gtggccattt tccctcaaaa cctggtactg tgcgtgcatg atgcctgcaa gggctcccat
     5341 aagtacacca cgctcgcctg tcaggtcgct gtacacctct ttttcaaaag tggtggggaa
     5401 gaggtagcca gagcctattg caattccaag tgcaagaacc ctttccgttg ccctgccggt
     5461 atagtcctga tagactgaat aactcgaatt gatacctgct cctgccagga aatttcggcg
     5521 t
//
LOCUS       DAOSNB010000168         5518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14865_length_5518_cov_4.802856, whole genome shotgun sequence.
ACCESSION   DAOSNB010000168 DAOSNB010000000
VERSION     DAOSNB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14865_length_5518_cov_4.802856"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..3195
                     /locus_tag="PLG42_07460"
     CDS             67..3195
                     /locus_tag="PLG42_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU31083.1"
                     /translation="MSEEILKALMELFALIVKQDTGLFIEERAYVHDFLSKQLSRESV
                     SEYLKLFDDLAGPVSIKVKDPEKESLAQHVTVRDSVKILGICKKINRNLNQEQKVVVI
                     LRLYELLNVNRQFTPQRMNIINTVSEVFKIQPDEVNAIEQFVRNEEPEKLENNKILVL
                     RTGDEKPKIHRKVLAGYHDTIIVFLRIPSVDLYFLKYNSPDQLYLNGLPIESGKIYSF
                     AKGSSIKSQFGQPVYYSDISASFLSEEVVHHINFTVENLTYRFSDGTTAVSNVSFSLE
                     GGNLIGILGSSGTGKTSLLNLMSGILKPTSGKIKINGIDINEDYKSIEGVLGYVPQDD
                     LLIEDLTVFENLYYAACQCFGGKSKEEIRKITDQMLINLGLWEKRNLKVGSPLKNIIS
                     GGQRKRLNIALELIREPSVLFLDEPTSGLSSHDSENIMDLLRDLTLKGKLIITVIHQP
                     SSEIFKLFDKIIILDQGGYMAYFGNPVEAVIYFKTLDAQINSSIGECPTCGNVNPEII
                     FNIIEAKVVDEYGRYTEQRKVKPQEWAAIFNEKRPILKIQEADEKPHSNLDRPSAFKQ
                     FLIYLVRDLKSKIANRQYILITLLEAPILGFILSFIIRYIPDPSSDVYIFFENENIPI
                     YIFMGIIVAVFLGLTISAEEIFRDRKILKRERFLSLNRHSYLLSKIAILIIISSIQVF
                     LFLIIANPILELRGLFGKYFIALFTTAFFANLIGLNISASFNSAITIYIVIPLIIIPM
                     MVLSGAMFPFDKLNRKISSVGKVPVIAELMPTRWTYEALMVSQFKDNKYSRFSESKYG
                     PTIYDFKKNISIANYYSVRLIPSLINALEKTDSIYVKTTSNTLNTGKLPLINDELKTS
                     GKLALIKNELMKIQKKFPNLKSFNYINDLTPEKYNKNLYEKTLEHLRYLSDNFNRAGN
                     KLSDSWDKFYIKNRDEIRRLENQYSNLKLQEIVTKFYERDKNKILEYKNSFIQNYDSV
                     YMDPDYEGFLSFRTHFFAPSKYFMGKMYDTFDFNIMLVLCCSIILYVTLYYELLARLV
                     NYIDRLKLKKVI"
     gene            3282..4178
                     /locus_tag="PLG42_07465"
     CDS             3282..4178
                     /locus_tag="PLG42_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31084.1"
                     /translation="MSFFTLAFLLAGCNSSTRKNTQFLFPDEDSIPLSQAEQLSDEAI
                     DDITRNISSPVEIANILQRMNVPFSNSYLASSIDASRITTSFDRALKLGILGADLGYL
                     NMYEKTGSSIDILSSIKKLADGINVGQFFDFETLKRLSLNRSNLDSLLFLSLESYNQI
                     DRFLREKGRGQLSALMVTGVWIEGQYLATQVLKQYPDTLLRSRVGEQKVILNDIIMLL
                     APYCNVSKEYTDLCRDMQELKEKYRNVKVTFTVGEPQMVEKDGALVVVQTDQSNVEMT
                     DEQLEAIINTTEKIRNRLISNE"
     gene            4171..4614
                     /locus_tag="PLG42_07470"
     CDS             4171..4614
                     /locus_tag="PLG42_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31085.1"
                     /translation="MSDMKKLVAFIMIIMLVPVVASGQASDPLVGKCVMNAGPNATYL
                     KDFRIQLGKASSAQDFRYKQVFALSKNMKYRFTLCNSENSQGELIMKLVDDTGRTILT
                     SFDPKTGKTFSSIEFVCNKTGTYKIMFDFKDFQQGLGVGVVSLVK"
     gene            complement(4645..>5518)
                     /locus_tag="PLG42_07475"
     CDS             complement(4645..>5518)
                     /locus_tag="PLG42_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31086.1"
                     /translation="DHRSMPLYFILRNLTGPGQIEIIISLAFILLISYLLVHYNTILF
                     FIPERTIVPSVLYAIFTGFFINYQTLHPVIPASIFMLIALRRIIDAYHVNGTAFSFFD
                     ASLLIGVGSLFYANLIWFGIIPLIGVIILKGYKLKEMMLSIAGIPVTLLITAGIYYLF
                     SPGISGLIENLRFNLFSEVDSYNFSIVELAGIATGGITLLAGIIFVFKRIKNMKIKSR
                     KIFILLLWWCLISVALYFISPAVSVEISYLAAIPSVYYISYYFIFSKSRIIPLAIFIA
                     FIVCASVIQALRYF"
BASE COUNT         1924 a         1034 c          989 g         1571 t
ORIGIN      
        1 ctgcaacaag ataagcccgg ccaagacttc tggctctgtc tttaaaattt agcacattta
       61 aaataaatga gtgaggagat tcttaaagcc ctgatggaac tctttgccct gatagtaaag
      121 caggacacag ggctttttat tgaagaaaga gcatatgttc acgattttct ttcaaaacag
      181 ctatcaagag aatcagtatc tgaatattta aagttattcg acgaccttgc cgggccagtt
      241 tctattaaag ttaaagatcc tgagaaagaa tccttagctc aacacgttac agtaagagat
      301 tcagttaaaa ttctgggaat atgtaaaaaa atcaaccgta atcttaatca ggaacagaaa
      361 gtagtagtga ttttaaggct gtatgagctc ctgaacgtaa acaggcagtt tacgccacaa
      421 cgaatgaata taataaatac agtatcagaa gtatttaaaa tacagccaga tgaagttaac
      481 gcaattgagc aattcgtcag aaatgaggag cctgaaaaac tggaaaataa caagatactg
      541 gttctgagaa ctggagatga gaaaccaaaa atccacagga aagttcttgc cggataccat
      601 gatacaataa ttgttttcct gaggatcccg agcgttgatc tatactttct aaaatataat
      661 tcacccgatc agttatacct taacgggctt ccaatagaat cggggaaaat atattctttc
      721 gcaaaaggca gttcaattaa aagccagttc ggtcagcctg tttattatag tgatattagt
      781 gcatcattcc tttcagagga agttgttcac catataaatt ttaccgtcga aaatctaacc
      841 tatagattca gtgatggtac cacagcagtg agtaatgtaa gcttttctct tgaaggcgga
      901 aatcttatcg gaattctcgg atcaagcgga acaggtaaaa cttccctgct aaatctgatg
      961 agtggtatac ttaaacccac atcaggcaag ataaaaatca acggtattga tattaacgaa
     1021 gattataaat caattgaagg tgttctgggc tatgtccctc aggatgactt gctgattgaa
     1081 gaccttacag tttttgaaaa cctttattat gcagcctgtc agtgctttgg agggaaaagc
     1141 aaggaagaga tcagaaaaat cactgatcag atgctcatca accttggcct gtgggaaaaa
     1201 agaaatctga aagtgggctc tcctttaaag aacataataa gcggagggca gagaaaacgg
     1261 ctaaacatag cacttgaact cataagagaa ccatctgtac tttttctcga tgaaccaacc
     1321 tctggccttt cttcacacga ttcagaaaat ataatggacc ttctgcgcga cctcacactg
     1381 aaaggcaagc taattattac cgtaattcat caaccatcgt ccgaaatatt taaactgttc
     1441 gataaaatca tcattcttga ccagggtggc tacatggctt atttcggaaa cccggttgaa
     1501 gctgttattt acttcaaaac ccttgatgcc caaataaatt catcaatagg tgaatgtccg
     1561 acctgcggaa acgtaaaccc tgaaattatc tttaatatca tcgaagcaaa ggttgtagat
     1621 gagtatggcc ggtataccga acaaagaaaa gtcaaaccac aggaatgggc agctattttt
     1681 aatgagaaac gaccaatttt aaaaattcag gaagcggacg aaaaaccaca cagcaacctt
     1741 gacagacctt ctgcatttaa acagtttctg atttatctgg tacgagatct gaaaagcaaa
     1801 atagcaaaca ggcaatacat tctaattaca cttctggaag ctccgatact tggttttatt
     1861 ctttcattta taatccgata catccccgat ccttcatcag atgtctacat ttttttcgaa
     1921 aacgaaaaca tacctattta tatatttatg gggattatcg ttgctgtgtt tctgggacta
     1981 acaataagtg ccgaagaaat tttccgtgac agaaaaattt tgaaaaggga aagattcctc
     2041 agccttaacc gtcatagcta ccttttgtca aaaattgcca tactgatcat tatttcatca
     2101 atacaggttt ttctctttct gataatcgca aatcctattc ttgagctcag aggactattc
     2161 ggaaaatatt ttattgcact ttttacaact gctttttttg caaatctgat tgggctgaat
     2221 atttctgcaa gctttaactc agccatcacg atatatatag ttattcctct gataataata
     2281 ccaatgatgg ttctcagcgg agcaatgttc ccattcgaca aactgaacag gaaaattagc
     2341 agtgtaggaa aagttcccgt tatagcagaa ttaatgccta cccgatggac atacgaagct
     2401 ttgatggtca gccagtttaa agacaataaa tatagccggt tcagcgaaag taaatacggc
     2461 ccaacaattt atgatttcaa gaaaaatatc agtattgcca attattattc cgtcagactt
     2521 attccttctc tgatcaatgc tctcgaaaaa acagattcaa tatatgtcaa aacaacatcg
     2581 aataccttaa acactggtaa actaccactg attaacgacg aactgaaaac ttcaggtaaa
     2641 ctggctctga taaagaacga actgatgaaa attcagaaaa aattcccaaa ccttaaatcc
     2701 tttaattata taaatgactt aactcctgag aaatacaata agaacctgta cgaaaaaacg
     2761 ctcgaacatc tcagatacct gagtgataat ttcaatagag ccggtaataa actatcagat
     2821 tcatgggata agttttacat taaaaacaga gatgaaatac gcaggctcga aaaccaatac
     2881 agcaatctga aactccagga gatagtaacc aaattttatg aaagggataa aaacaaaata
     2941 ctggaatata agaactcctt cattcaaaat tatgattccg tttatatgga tcctgattac
     3001 gaaggatttc tgtcattcag aacgcatttt tttgcaccct caaaatattt tatgggtaaa
     3061 atgtacgata cattcgattt taatattatg ctggttcttt gctgttcaat tattctgtac
     3121 gtgacgcttt actatgaact tcttgccaga ttggttaatt acattgaccg acttaaacta
     3181 aaaaaagtca tataataaac tctgtttata tttttttatt tatatttttg tacttaacta
     3241 atttacaatt attaaaaata atgaccggca ggttactgtt aatgtctttt ttcactctgg
     3301 catttttact ggccgggtgt aactcgtcga caagaaaaaa tactcagttt ttatttcctg
     3361 acgaagattc tatacccctg tcgcaagctg agcaactgag tgatgaagca atagatgaca
     3421 taactagaaa catttcatct ccggttgaaa tagcaaatat cctgcagcgg atgaatgtgc
     3481 ctttttcaaa cagttatctg gcatcctcaa tcgatgcatc cagaataaca acaagtttcg
     3541 acagggcatt aaagttgggt atcctcggag cagatctcgg ataccttaat atgtatgaaa
     3601 aaacaggcag ttccattgat attctgtctt caattaaaaa acttgccgat ggtattaatg
     3661 tcggtcaatt ttttgatttt gaaactctta aaagactttc actgaacagg tcaaatctcg
     3721 attcgcttct tttcctgtcg cttgaatcct ataatcagat agacagattc ctccgtgaga
     3781 aaggaagagg ccagctttca gcacttatgg tcaccggagt ttggatcgaa ggtcaatacc
     3841 tggcaactca ggtgctcaaa caatatcccg atacccttct caggagcagg gtcggagaac
     3901 aaaaggttat cctcaatgat attataatgc tccttgcacc atactgtaac gtaagcaaag
     3961 agtacactga tctatgcagg gatatgcagg aactcaagga aaaatacagg aacgttaaag
     4021 ttacttttac agtaggtgag cctcaaatgg ttgaaaaaga cggagcactg gttgttgttc
     4081 aaaccgacca gagtaatgta gagatgactg atgaacaact tgaagcaatt attaacacta
     4141 cagaaaaaat ccgaaacagg ttaatttcaa atgagtgata tgaaaaagct tgttgctttt
     4201 ataatgataa taatgcttgt gcctgttgtc gcatcaggcc aggcaagcga tccgcttgtg
     4261 ggaaaatgtg taatgaacgc aggtccaaat gccacttatc tgaaagactt tcggatccag
     4321 cttggtaaag catcatctgc ccaggatttc agatataaac aggtatttgc cctgagcaag
     4381 aacatgaaat accgctttac cctctgcaat tcggaaaact cgcagggaga acttataatg
     4441 aaactcgttg acgacacagg gagaaccatt ttaacttcat ttgaccctaa aaccggaaaa
     4501 accttctctt caatcgaatt tgtatgcaac aaaaccggta catataaaat tatgttcgat
     4561 tttaaagatt ttcagcaggg acttggggta ggagtcgttt cacttgttaa atagtctcca
     4621 gctacagcaa ctattatttt atcactaaaa atatcgcaaa gcctgaatta ctgaagcaca
     4681 cacgataaat gcaataaata ttgcaagagg tattattctg ctcttgctga aaataaaata
     4741 gtatgaaata taatagactg aaggaatagc tgcaagatat gaaatctcaa ccgaaactgc
     4801 cggtgaaata aaatataatg caacagatat taaacaccac cataatagaa gtataaatat
     4861 ttttcttgac tttattttca tattttttat ccgcttaaaa acaaaaataa tcccggcaag
     4921 caaggtaatt cctcctgttg caattcctgc aagttccaca attgaaaaat tatatgaatc
     4981 aacttcactg aaaagattaa atcttaaatt ttcaatcaat ccgctaatgc cgggggaaaa
     5041 cagatagtaa attcctgcag ttatcagtaa agttaccggt ataccagcta tagaaagcat
     5101 catttctttc agtttataac ctttaagaat tataaccccg atcaagggaa taatgccgaa
     5161 ccatatcaga tttgcataaa aaagacttcc gactcctata agcaaggaag catcaaaaaa
     5221 actgaaagcc gttccgttta catgatatgc atcaataatt cttctcaatg cgatcagcat
     5281 aaaaatcgaa gcaggtataa ccggatgcag cgtctgataa ttaatgaaga atcctgtaaa
     5341 aatggcataa agcaccgaag ggacaatcgt tctttcagga ataaaaaaca gaatcgtgtt
     5401 gtaatgaaca agcagatagg aaattagaag aataaatgcc aaacttatta taatttctat
     5461 ttgaccaggg ccagttaaat tcctgagaat aaaatagaga ggcatggatc tgtgatcg
//
LOCUS       DAOSNB010000169         5504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14918_length_5504_cov_4.273628, whole genome shotgun sequence.
ACCESSION   DAOSNB010000169 DAOSNB010000000
VERSION     DAOSNB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14918_length_5504_cov_4.273628"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLG42_07480"
     CDS             <1..761
                     /locus_tag="PLG42_07480"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31087.1"
                     /translation="INWIHRRTGDSDIYFIVNSSDKPCEINVSFRVSGMEPEKWDPVN
                     GETGPAGYFVTGNRTVVPLSLEGKQSTFIVFRNKTERNERVIEEPPATLMAVLSGPWE
                     VTFPAGLGAPGKITLDTLYSLSLHNDDGVKYFSGTATYRTTFKVPGRWLKNNPLLLLD
                     PGKVCDIAEVSVNGSPYEVIWTRPYAANITGLLRKGINTIEIKVTNQWTNRLAGDAKL
                     PPDKKVLKSQIFVRPGIINESGLIGPVRIIRISE"
     gene            773..2077
                     /locus_tag="PLG42_07485"
     CDS             773..2077
                     /locus_tag="PLG42_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOU31088.1"
                     /translation="MKTHKLITVIFLTICFLPGIFSQQNAPPKLVAGIPVNYDEALVG
                     SYTLPELLVTLNGKKVRNTKMWYNVRRPEIVRMFEELEYGKTPGRPADMSFNVFDKGT
                     PALDGKAIRKQVTVYFTGDTSKYKMDILIYLPAGASGPVPLFLNLGFMPNNTITDDPG
                     IKPGYMWSRDGKRIPAKAGSFGRMNVDLFLSNGIGFASINYADIEPDFADGYKYGIRG
                     YYLKPGQKYPADDEWGAIAAWSWGLSRAMDYFETDPQIDSKKVILFGVSRLGKTVLWT
                     GARDTRFGMVIASCSGEGGAALSRREYGENIKHMTDSTRYFYQFCGNRRKFADDPSSC
                     PIDAHMLVALVAPGPLLLQTGETDYWSDPKGEFLAAVASEPVWKLLGKKGLGTDVMPP
                     AGKSILNDLGYYMHAGGHGAMPDDYQIFIKFIRKHFFGEKIE"
     gene            2319..4706
                     /locus_tag="PLG42_07490"
     CDS             2319..4706
                     /locus_tag="PLG42_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOU31089.1"
                     /translation="MKKAPFDDKLNDKLLKILEEERASRKAIQESETKFRTLFESAID
                     AIFIMDDDKFIDCNQSTLKIFGCSSHPEILGLPPYEFSPPLQPDGRDSREKALEMINR
                     AFNGENLRFYWKHAKKDGTAFDAEVTLNRFMVNDKYYLLAIVRDIDEITAIQEKLHLS
                     EERYRLISEVAYDYMFSAIVKDDNTFDLDWVAGAFEKITGYTFEEFKAIGGWQSTVHP
                     EDMETAKHNFELLKRNRKVISELRRIKKNGDIVWVKFYAHPVFDEANKKLIRIYGAVQ
                     DITERKNAEFALKQLEENFHRSIAESPLGIRIVTPEGVTVYANKSFLDIFGYRNIEEY
                     DLTPSIEKYTTASYSQHVLRKEKRRIGEDVLDYEISIRRKDGEIRDLKVIRKEILWNG
                     KKHFQVIYQDITEQNNLTRELIAAKEKAEESNRLKTAFLQNVSHEIRTPMNSIMGFME
                     LLKNPNMLREERESFIRMIQESGERLLRTINDIIEISKIETGIVDVRVTTEDLYSIIQ
                     YHYNFFRHQAEEKGLAFLLSFPDDLKDCKIKVDRFKLDGILINLISNAIKFTKKGFVE
                     LGCYQEGDNMIFFVRDSGTGIPEEKQKAVFEPFVQANMDISRPYEGSGLGLSITRAYV
                     KMLKGILWLESKENVGSTFYFSIPVYSETKITEITTEPASAEKEVHEPGSSMKILIAE
                     DDDASYLFLKTLLDRLGIKEIRAKNGIEVLEIMKNHEDISIILMDLKMPLMDGFEATR
                     KIREFNTTIPIIAQTALAFPSDSERALNEGCNDYIAKPIDRNELLALIKKYSAQR"
     gene            4815..5381
                     /locus_tag="PLG42_07495"
     CDS             4815..5381
                     /locus_tag="PLG42_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOU31090.1"
                     /translation="MFDDLYLYLVKKYKFTQKLVTALLLAVLSIFPGKAFSQNIYDTL
                     HKKPERFVIVSDDNYPPFIFRDEDGNLTGLLIDEWNLWQKKTGTLVEWLATDWNKALE
                     IMRSGGADVIETVFYTEERAMWLAYSRPYVQIPVPVYFHRNLSGISDISTLKGFTIGA
                     KAGDAVIEYIRKNNFQLDKKWQTCKANA"
BASE COUNT         1837 a         1040 c         1170 g         1457 t
ORIGIN      
        1 aaatcaactg gatccaccgc aggacaggag attcagatat ttacttcatt gtaaattcgt
       61 cagacaaacc gtgtgaaatt aatgtaagct ttcgcgtttc ggggatggaa cctgagaaat
      121 gggatcctgt taacggagaa acaggtccag caggttattt tgtaactggc aaccgtactg
      181 ttgtcccgct tagccttgaa gggaaacagt caacattcat cgttttcagg aataaaaccg
      241 aaagaaatga aagagttatt gaggagcctc ctgccacact catggctgtg ctttcagggc
      301 catgggaagt aactttccct gcagggttgg gtgcacccgg taaaattacg ctcgatactc
      361 tctactcact gtcgctccat aatgatgatg gcgtaaaata tttctcaggt acagccactt
      421 accggactac tttcaaagtt ccaggtagat ggctcaaaaa caatcctctc ctgctgctcg
      481 acccgggtaa agtatgcgac attgcggaag tatcggtaaa cggctctcct tatgaggtaa
      541 tatggacacg gccatatgct gcaaatatca caggccttct cagaaaaggg ataaatacca
      601 tagaaataaa agtaaccaac cagtggacta acaggctggc tggtgacgca aaacttcctc
      661 ctgacaaaaa agtactcaaa tcgcagatat ttgtcaggcc gggaattatt aacgaatctg
      721 gtcttattgg acctgtaaga atcataagaa tttcagaata aaatcttcag acatgaaaac
      781 tcataaatta attactgtta tttttttaac aatctgtttt ttacctggaa ttttttccca
      841 gcagaatgcc ccaccaaaac tggttgcagg cataccggta aattatgatg aggcgctggt
      901 tggctcatac actctgcctg agcttcttgt aaccctgaat ggcaaaaagg tcaggaatac
      961 caagatgtgg tacaatgtgc gccggcccga aattgtaagg atgtttgaag agcttgaata
     1021 cggtaaaact ccaggcagac ctgcagacat gtcatttaat gtgttcgaca aaggaacacc
     1081 tgctcttgac ggtaaagcca tcagaaaaca ggtaacggta tattttaccg gcgacacatc
     1141 aaaatataaa atggacatac tcatttatct gccggctgga gcttcagggc ctgttccgct
     1201 tttccttaac ctcggtttta tgcccaacaa tacaataact gatgatccag gtataaaacc
     1261 aggttacatg tggagcaggg atggcaaaag gatacctgca aaagccggca gtttcggaag
     1321 aatgaatgtt gacctgtttc tatcgaacgg tataggcttt gccagcataa attatgctga
     1381 tatagagccc gatttcgccg atggctacaa atacggcatc aggggttatt acctgaagcc
     1441 agggcagaaa tatcctgccg atgatgaatg gggagccata gccgcatggt catgggggct
     1501 cagcagggca atggattatt tcgaaacgga tcctcagata gattcaaaga aggttattct
     1561 gtttggggtt tcgaggctcg gaaaaacagt tttatggaca ggagcgcgcg acacccgctt
     1621 cggcatggtt atagccagct gttcgggcga aggaggagca gcattatcgc gacgtgaata
     1681 tggggagaac ataaaacaca tgaccgattc cacaaggtat ttttatcagt tctgtggcaa
     1741 ccgcaggaaa tttgccgacg acccgtcgtc atgcccgatt gatgcacata tgctagtggc
     1801 actcgtagca cccgggcccc tccttcttca gacaggcgaa acagattact ggtccgaccc
     1861 gaaaggcgaa ttccttgctg ctgtggcatc tgaacccgtc tggaaactgc tgggaaaaaa
     1921 aggtctgggt acagatgtta tgccccctgc aggtaaatca atacttaacg acctgggcta
     1981 ttatatgcac gcaggaggcc atggggctat gcctgacgat tatcaaattt ttataaaatt
     2041 tatcagaaaa cactttttcg gggaaaaaat agagtgatta ttaaatactc tatcaatgtt
     2101 ttcagaatta tgttgtcata atcaatatgt tataagaatg attatttaga tgaaaataaa
     2161 tttacatgat tttaactatt taattgcaaa cctttacctt tgttgcggat taaggtaaat
     2221 tctgagtttg atttttgttt ttacccttta accggggaaa gggtaattaa ttatatgctt
     2281 cagaattaac caaaagcagt ggtttaactt ttaggtcaat gaaaaaggcg ccttttgatg
     2341 ataaattgaa cgataagctc ttaaaaatcc tggaagagga gcgggcttca aggaaagcaa
     2401 ttcaggagag tgaaacaaaa ttcaggacac tttttgaatc agccatcgat gccatcttca
     2461 taatggatga cgacaagttc atcgactgca atcagtcaac cttgaaaatt tttggatgct
     2521 caagccatcc agaaatactt ggtcttccac cctatgaatt ttccccacca ttgcaacccg
     2581 acggaaggga ttcccgtgaa aaagcgctgg aaatgattaa cagagcattt aatggtgaaa
     2641 atctcaggtt ttactggaaa catgcaaaaa aagacggtac tgcttttgat gcagaagtaa
     2701 ccctcaacag gtttatggtc aatgataaat attaccttct cgcaatagtc agagatattg
     2761 atgaaataac agctatacag gaaaaactcc acctgagtga ggagagatat cgactgattt
     2821 cggaagtagc ttatgattat atgttctctg caatagttaa agatgacaat acttttgacc
     2881 ttgattgggt ggctggggcg tttgaaaaaa ttaccggtta cactttcgaa gaatttaaag
     2941 caattggtgg gtggcagtca acagtacatc cagaggatat ggaaactgca aaacataatt
     3001 ttgaactgct taagagaaac cgaaaagtaa tttcggagtt acgaagaatt aagaaaaatg
     3061 gtgatattgt atgggttaaa ttttatgcac atcccgtttt tgatgaggca aataaaaaac
     3121 ttatcagaat atacggagct gtacaggata taactgaaag aaaaaatgct gagtttgccc
     3181 tcaaacaact tgaagaaaac tttcaccgct caatagctga atcacccctc ggcatcagaa
     3241 tagtaacacc tgaaggagta acagtttatg ccaacaaatc gtttctcgat attttcggtt
     3301 acaggaatat tgaagaatat gatcttaccc catcaattga aaaatatacc accgcgagtt
     3361 attctcagca cgtgttaagg aaagaaaaaa gaagaattgg tgaggacgtt cttgactacg
     3421 aaattagtat aagaagaaaa gatggggaaa taagagattt aaaagtaata agaaaagaaa
     3481 tactctggaa cggtaaaaag cattttcagg ttatttacca ggacatcact gaacagaaca
     3541 accttacccg ggaacttatt gcagcaaaag aaaaagcaga agaaagcaac aggcttaaaa
     3601 ctgcattcct gcagaatgtg tcacatgaga taagaactcc gatgaactct atcatgggat
     3661 ttatggaact tttgaaaaac ccaaacatgc ttcgtgaaga gagggaaagc ttcataagaa
     3721 tgattcagga aagtggtgag cgacttctca gaactatcaa tgatatcata gaaatctcca
     3781 aaattgaaac agggatagtt gacgtaaggg ttacaactga agatttgtat agcatcattc
     3841 agtatcacta taattttttc agacatcagg cagaagaaaa aggccttgct ttccttctct
     3901 cgttcccgga tgacctgaaa gattgtaaaa taaaagttga cagatttaaa ctcgacggaa
     3961 ttcttattaa cctcataagc aatgccataa aatttacaaa aaaaggattt gttgaactgg
     4021 gctgctatca ggaaggtgat aatatgatat tctttgtcag ggattctggc actggaattc
     4081 cggaagaaaa acagaaagct gtttttgaac catttgttca ggcaaatatg gatatctcaa
     4141 gaccatacga aggttcagga ctgggtctgt caataacacg tgcgtatgta aagatgttga
     4201 aaggaatatt atggcttgaa tcgaaggaaa atgtgggtag cacattctat ttctcaatac
     4261 cagtttattc tgaaacaaaa attacagaga taacaaccga accagcctca gcagaaaaag
     4321 aagtgcacga accaggatct tccatgaaaa tattaatcgc tgaggatgat gatgcaagtt
     4381 accttttcct gaaaacactt cttgacagat tgggaattaa agaaatacgg gcaaaaaacg
     4441 gaatagaagt tctcgaaata atgaaaaacc atgaggatat ttcaattata ctgatggatc
     4501 ttaagatgcc cctgatggat ggatttgaag caaccagaaa aataagagaa tttaacacta
     4561 ccattcccat aattgcccaa acagcgcttg cattcccatc cgacagcgaa agagcactta
     4621 atgaaggatg taatgattat attgcaaaac caatcgacag aaacgaactt cttgccttga
     4681 ttaagaaata ttctgctcag cgttaagcac ccgttattat cttacaattt ataatattat
     4741 cccaaaattt ccattttcaa cttttggaaa tattgaaaat caactatttt tgcactgtat
     4801 ttactgccca ggaaatgttt gatgatcttt acctgtattt agtaaagaaa tacaaattta
     4861 ctcaaaagct tgtaactgct ttattgctgg cagttctgtc aatatttccg ggcaaagctt
     4921 tttcacaaaa catatacgat acactacata aaaaacctga aagatttgta attgtatcgg
     4981 acgataacta tccaccattt attttcaggg atgaagacgg aaacctgacc ggtttgctca
     5041 ttgatgaatg gaacctgtgg cagaaaaaaa ccggtacact tgtagagtgg ctggcaaccg
     5101 actggaataa ggcactcgaa ataatgcgtt cgggtggcgc cgacgtaatt gaaacagtat
     5161 tctatactga agaaagagcc atgtggctgg catattccag accgtatgtt caaattcccg
     5221 ttccggtata ctttcatcgt aacctgagcg gaatatcaga tatctcaacg ctcaaaggtt
     5281 ttaccatcgg tgccaaggca ggagacgctg ttattgaata tatcagaaaa aacaactttc
     5341 aactggataa aaaatggcag acttgcaaag ccaatgcgta acgtttatgg cgcaagaaga
     5401 gtgcgtgtta attggagcgt tggcgaagta aaaaaagcac tgagtaacgc tcacaatcaa
     5461 gggttaagaa atggctaaag caaactacga ccacattata ccaa
//
LOCUS       DAOSNB010000170         5494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14954_length_5494_cov_4.670712, whole genome shotgun sequence.
ACCESSION   DAOSNB010000170 DAOSNB010000000
VERSION     DAOSNB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14954_length_5494_cov_4.670712"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2424
                     /gene="bamA"
                     /locus_tag="PLG42_07500"
     CDS             91..2424
                     /gene="bamA"
                     /locus_tag="PLG42_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOU31091.1"
                     /translation="MLISISGLKTGQAITIPGEEISSAAKKLWNQGLFSDVRISIMEM
                     RNDTVFLDIFLQERPRLSSLKITGLSRNETQDIREKIKLPAGSQITAFILNNTEKIIK
                     DHFVEKGFHNTTVNFIQKDDPDKPNSVILTIEVNKNEKVKIGEIQFVGNQAFDDRKLK
                     RQMKGTKEKNWNFFRSSKYIEDKFAEDKEKLYTFYNDHGYKDFTILSDSVYRISSDRL
                     GVLIKVDEGRQYFIRNITWVGNSIYPTRLLDQVLNIEKGSVYNKSLLNKRLKIDEDAV
                     SSLYLDNGYLFSNLTPVEVKVEGDSVDIEVRIFEGDQAYLNDVVITGNTRTNEHVARR
                     ELYTLPGDLFSKEKILRSVRQLGVLGHFDPEKINPQPIPDPMNGTVDLLYPLEERAND
                     QFEISGGWGAGMLVGTVGVRFNNFAMKNFFNLSEWRPYPSGDGQSLSIRAQSNGRIYQ
                     SYNISFVEPWLGGKSPNTFSVSFYRSVMTNGRKRNEDGRQSMIIDGASIGLGKRLEWP
                     DDYFSLYGELNFQRYDLNNYNVFRFLFENGTSNLFSVTARLMRFSTAPNLIYPRSGSS
                     FSLQLQVTPPYSLINGRDYKGATDQVKYRWIEFHKWVFKADYYFPITRNDKLVLNTKF
                     AFGYLGFYNKDIGPSPFENFYLGGDGMTGYSFYGREVIALRGYTNGSLTPSDPVKGSP
                     AGNVYSKITFELRYPITLNQQATIYALTFLEAGKAWYELKEYNPFRMHRSAGVGLRAN
                     LPMFGLLGIDWGYGFDAVPGNPDANKSQFHFVIGQQF"
     gene            2451..2966
                     /locus_tag="PLG42_07505"
     CDS             2451..2966
                     /locus_tag="PLG42_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU31092.1"
                     /translation="MKKIILLTAAIVLASAFATAQKYAFVDTDYIRKNIPAFNTAQEQ
                     LDKLSKQWEQEIADGYAEVEKMYKAYQNEAVLLSQEMKTKREEAIINREKEVKELQNK
                     YFGIEGELFKKREELIKPIQDEILKAIKEIAVEGSYAVIFDTAAGGNVLYANPKYDIS
                     DQVLEKLGYRK"
     gene            3078..3599
                     /locus_tag="PLG42_07510"
     CDS             3078..3599
                     /locus_tag="PLG42_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOU31093.1"
                     /translation="MKKITAIILTGVIFLITAQLTTGQTLKFGHINTDELIRAMPEYD
                     TALAKLNRLQKELTNTLELMQVELNNKIEAYNKESKLLSDLVRQTKEQEIADMNRRIQ
                     DFQLQAQSQLQEEQANLTRPIFEKVDKAIRDVGKEGGFIYIFDVANRQLLYFDETKSV
                     NVTAQVKAKLGLK"
     gene            3693..4499
                     /gene="murI"
                     /locus_tag="PLG42_07515"
     CDS             3693..4499
                     /gene="murI"
                     /locus_tag="PLG42_07515"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOU31094.1"
                     /translation="MDLKRQPIGIFDSGVGGLTVAHAIKQLMPGESLVYFGDTAHLPY
                     GDKSAELICQYSEKIVEFLLGQNAKMILIACNSASASAYDYLQKKFGKDTIILDVISP
                     VIEYVSSRNFKKIGVIGTKRTIASGVYEKMIALKSPETKVVSLATPLFVPMIEEGFIF
                     DDISNQIIRAYLSNQLFEGIEALILGCTHYPIIRNQISKFFDFRVEIIDSSRVVSSVV
                     RDILQKNNIQNDSRLVNDRFLVSDYTEYFRKIARLFFEKEINLEKTDIWS"
     gene            4667..>5494
                     /locus_tag="PLG42_07520"
     CDS             4667..>5494
                     /locus_tag="PLG42_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOU31095.1"
                     /translation="MGRKPQFIEKDEVVIRFSGDSGDGMQLTGTLFSDTAALYGNDLA
                     TFPDYPAEIRAPQGTVGGVSGFTVHLGHSEVNTPGDYADVLVAMNPAAIKANAQWLKE
                     GSTIIYDSDSFTESNIRKAGYITDPIKEENLQDYNVIAAPITSMVKEALKNFGLDPKS
                     VLRTKNMFALGMVYWMFDRKLSYTEDYCDKKFKNRPEIAEANKIALRAGYYYAETIEA
                     LTPVRINPAPIEKGKYRNINGNTATAWGFIAAAEKAGLKLFVGSYPITPATGILEELA
                     "
BASE COUNT         1824 a          991 c         1207 g         1472 t
ORIGIN      
        1 aggaaagagc tgagatatat gattacaggg agattgatga atacataatc ggaggaatta
       61 caatttcagg tgtaaaatat ctggattcca atgctaataa gtatttccgg gctaaagaca
      121 ggacaggcaa ttacaatacc aggggaagaa atatcctcag cagctaaaaa actctggaac
      181 cagggcctct tttctgacgt caggatatca attatggaaa tgagaaatga cacagttttt
      241 ctcgatattt ttcttcagga acggccaagg ttgtcaagtc ttaaaattac agggctttca
      301 agaaatgaaa cgcaggacat cagggagaag ataaagctac ctgccggctc acagataact
      361 gcattcatat taaataatac tgaaaagatt ataaaagacc attttgttga aaaaggtttt
      421 cacaatacta cagtaaattt tatacagaag gatgatcctg acaaacccaa cagtgtgatt
      481 cttacaattg aagtaaataa gaacgagaaa gtcaaaattg gtgagattca gtttgtggga
      541 aatcaggcct ttgacgaccg caaactgaaa aggcagatga agggtacaaa agaaaagaac
      601 tggaactttt tcaggtcctc aaagtatata gaagacaagt ttgcggaaga caaggaaaaa
      661 ctctatacct tttataatga tcacggatat aaggacttta cgattctttc tgattcagta
      721 tacaggatat catccgaccg tttgggtgtg ttaataaaag tagatgaagg ccgacaatat
      781 tttataagga atataacatg ggtaggcaac agtatttatc ctacaaggtt gctcgaccag
      841 gttctaaata ttgaaaaggg ttcggtatac aataagagtc ttcttaacaa acggctcaag
      901 attgacgaag atgcagtaag ctcactctat ctcgacaatg gttatctctt ttccaatctt
      961 actccagtgg aggttaaggt tgaaggtgat tcggttgaca ttgaagtaag aatatttgaa
     1021 ggtgaccagg catatctgaa tgatgtggta atcactggca atacgaggac caatgagcat
     1081 gtggcaagga gggagcttta cacattgccc ggggatcttt tcagtaagga gaaaatatta
     1141 cgttctgtac gacagcttgg tgtgctgggc cattttgacc cggaaaaaat aaacccccag
     1201 cccatacctg atcccatgaa tggaaccgtt gaccttctct acccccttga ggagagggca
     1261 aacgaccagt ttgaaatatc cggtggctgg ggcgcaggaa tgttagttgg gacggtggga
     1321 gtaaggttca ataattttgc catgaagaac ttctttaacc tgagtgagtg gagaccttat
     1381 ccttcgggcg atgggcagtc gcttagtata agagcacagt cgaacggacg tatttaccag
     1441 tcgtataaca tttctttcgt tgagccatgg cttggaggta aaagtcccaa tacattttca
     1501 gtctcattct acaggtcggt aatgacaaac ggcagaaaga gaaatgaaga cggacggcaa
     1561 tcgatgatta tcgacggggc ttcaataggc ctcgggaaaa gacttgaatg gcccgacgac
     1621 tatttctcac tttatggtga gcttaacttt cagcggtatg acctgaacaa ctataatgtg
     1681 ttccgcttcc ttttcgaaaa cggcacttcc aatttgttca gtgttacagc acgactgatg
     1741 agattctcaa cagctccgaa ccttatctat ccgagaagcg gttcgagttt ctcactgcaa
     1801 cttcaggtaa caccgccata ttctctgatt aacggaagag attataaagg tgcaactgac
     1861 caggtaaaat acagatggat cgaattccat aaatgggtat tcaaagccga ttattatttt
     1921 ccgattacaa gaaatgacaa acttgtactt aatacaaaat ttgcattcgg ataccttggc
     1981 ttctataata aagacattgg accttcacct tttgagaatt tctatctggg tggcgacggt
     2041 atgacggggt acagcttcta cggacgtgag gtgattgcac tacgtggtta cacaaatggt
     2101 tcccttaccc caagtgatcc agtgaaaggt tctccagcag gaaatgttta ttccaaaatt
     2161 acatttgagc tcaggtatcc tattacgctt aatcagcagg caactattta tgcccttaca
     2221 ttccttgagg caggtaaagc atggtatgaa ctgaaagaat ataatccttt caggatgcac
     2281 aggtcggccg gagtgggatt gagagcaaac ctgcccatgt tcggactgtt gggcattgac
     2341 tggggatatg gatttgatgc cgtgcccgga aatcccgatg caaataaatc gcaattccat
     2401 ttcgtgattg gccagcaatt ctaattgtta aacctaaaaa acagaaaatt atgaaaaaga
     2461 taattctatt aaccgcagca atagttctgg cttcggcttt tgccactgca cagaaatatg
     2521 catttgtcga taccgattac atcagaaaaa atatccctgc attcaatact gcccaggaac
     2581 aacttgataa actgtcaaag cagtgggaac aggagatagc tgatggttat gcagaagttg
     2641 aaaaaatgta taaggcatac cagaacgaag cagtattgct atcacaggaa atgaaaacca
     2701 aacgtgaaga agcaataatt aacagggaga aggaagtaaa agaactgcag aataaatatt
     2761 tcggcataga gggtgaactt ttcaaaaaac gtgaagagct gattaaaccc attcaggacg
     2821 aaatccttaa agcaatcaag gagattgcag ttgaaggaag ctatgcagta atattcgata
     2881 cagcagcagg tggaaatgtg ctatatgcaa accccaagta cgatatcagc gatcaggtgc
     2941 ttgaaaaact cggatacaga aaataattaa ttttcagaca gttttaacaa aacattaaga
     3001 aaataataag ctttggaatc aatagatttt agttaaataa ttatttttgt gaagatttaa
     3061 ttaataaact gtaagtaatg aaaaagatca cagcaattat tttgacggga gttattttcc
     3121 tgattacagc tcagctgact acaggccaga cattgaagtt cggacatatt aatactgacg
     3181 aacttataag agccatgcct gaatatgata ctgcacttgc aaaactaaac aggttgcaga
     3241 aagaactgac caacacattg gaactgatgc aggttgagct taataataaa attgaagctt
     3301 acaataagga aagcaaactt ttatctgacc ttgtgcgcca gacgaaggaa caggaaattg
     3361 ctgacatgaa cagacgtatt caggattttc agcttcaggc ccaaagtcag ctacaggaag
     3421 aacaggcaaa cctgacaagg cctattttcg aaaaagtcga taaagcaatc agagacgttg
     3481 ggaaagaagg cggttttatc tatatttttg atgttgccaa caggcaattg ctctatttcg
     3541 acgagacgaa aagcgttaat gtaacggcac aggtaaaagc caagctggga cttaaatgaa
     3601 gtagaggctg atataatcat agcatccttc aggattaact tcccgaagga tgttttttta
     3661 ttaatatcac gaaaagcata ttttctttct tgatggatct taaacgacaa ccaataggaa
     3721 tctttgattc aggagttgga ggactgacgg ttgctcatgc aataaaacaa ctgatgccgg
     3781 gcgaaagtct tgtctatttt ggcgatactg cacacctgcc atacggtgat aaatcggctg
     3841 aactcatctg ccagtattct gagaaaatag tcgaatttct tcttggccag aatgcaaaga
     3901 tgattcttat tgcatgtaac tctgcttctg cttcggcgta tgactatctg cagaaaaagt
     3961 ttggaaagga cacaataatt ctggatgtca tatctcctgt gatagaatat gtcagcagcc
     4021 ggaatttcaa aaaaataggt gttataggta caaaaaggac aattgcttcg ggtgtttatg
     4081 aaaaaatgat tgccctaaaa tcccctgaaa cgaaagtggt atcactcgca acacctttat
     4141 ttgttccaat gatagaggag ggatttattt ttgatgatat aagcaatcag attataaggg
     4201 cttatctttc caatcaatta tttgaaggta tcgaggccct gatacttgga tgcacacact
     4261 atccgataat acgtaatcag ataagtaaat tttttgactt tcgcgttgaa ataatcgatt
     4321 cttcaagggt tgtttcatca gtggtgaggg atatacttca gaaaaacaac atccagaatg
     4381 attcccgcct ggtaaatgac aggtttctgg tttcagatta tacagaatat ttcaggaaaa
     4441 ttgccagact attttttgag aaggaaatca atctggaaaa aactgacatc tggtcatgat
     4501 ggttctaatt ataccttaaa aaagtatgac caaagtcata cagtaaatat gatatttatc
     4561 tgaactttcg attattaatt caattaattt tgcattgaaa aaacaaaaga tactgttaaa
     4621 ttgttaacag tttatattta ctgataaaat ttatagaggt aagcagatgg gcaggaagcc
     4681 tcagtttatt gaaaaggatg aagttgttat aaggttttcg ggagattccg gcgacgggat
     4741 gcagcttacg gggacacttt tctcggacac agcagcgctc tatggaaatg atctggcaac
     4801 ttttccggat tatccagccg aaataagggc tccgcagggc actgtgggag gagtgtcagg
     4861 gtttacggtt catctgggtc attccgaagt taatactccc ggagattatg ccgatgtact
     4921 tgtggcaatg aacccggcag ccataaaagc caacgcgcag tggctcaaag aagggagcac
     4981 tataatttac gacagtgata gttttactga aagcaatatc aggaaagcag gatacattac
     5041 tgatcctata aaagaagaaa atctccagga ttataacgtt atagcagctc caatcacctc
     5101 gatggtgaaa gaggctttga aaaattttgg actcgaccca aagtcggtcc tgagaaccaa
     5161 gaatatgttt gctctgggga tggtttactg gatgttcgac aggaagcttt cgtataccga
     5221 ggattattgt gacaaaaaat tcaaaaatcg ccctgaaatt gctgaagcta acaaaatagc
     5281 cctgagggca ggatattatt atgctgagac gattgaggca ctcacaccag taagaataaa
     5341 ccctgccccc attgaaaaag gcaaatacag aaatatcaac ggtaacacag ctacggcgtg
     5401 gggtttcatt gccgctgccg aaaaagcagg attaaaactc tttgtcgggt cgtatccaat
     5461 tacgcctgca acaggcattc tggaagagct tgca
//
LOCUS       DAOSNB010000171         5493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_14961_length_5493_cov_3.354542, whole genome shotgun sequence.
ACCESSION   DAOSNB010000171 DAOSNB010000000
VERSION     DAOSNB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_5493_cov_3.354542"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PLG42_07525"
     CDS             <1..1842
                     /locus_tag="PLG42_07525"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31096.1"
                     /translation="DYRLTIAELILDNFTKKWTEWAHKNGKLTRNQAHGSPGNILDLY
                     AAADIPETEGTEIFRMKFATSAANVTGRKLASAEAATWLDEHFSSTLLDIKKAVDKFF
                     VAGVNHIVYHGTCFSPPDEPWPGFHFYAAVELKPSNPIWTDFHALNEYVTKVQSFMQS
                     GRPDNDLLLYFPASDTYASYNKMMLEHFDGTGQRFRDTWFRKAAEKMTSRGYAYDFIS
                     DQQLMKCTTAGKEILTEGNNLYKAVIVPQCKYIPVETFNKLCNLAEEGAVVIFYGNLP
                     ENVSGFAGLEVNRDIFNYLKEQLKFSETNREGVRVAVSGTGKLFQGDSLENLLVSAGI
                     RRESVTDQGLQFFRRKNSEGTIYFINNPSDKYFEGNITLETPAKSAGIFNPMTGEKGK
                     VKVTGSKDGKIKLYLQLKPFESLIIKAFKTAVQVPDYLFYEKSGDPVVINGKWNLRFI
                     EGGPVLPASVSLNEPVLWTALEGEEYKDFSGSAVYSTTFTALAFKSKFLKLNLEKVCE
                     SACITLNGEYIGTLLGPDFSVVIPAEKLKKTNNIEIRVTNLAANRIAYMDRKGIEWKK
                     FYNTNFPSRLPQNRKEGLFDASAWQPRPSGLQGEVCLIPLKPVTNSR"
     gene            1944..4196
                     /locus_tag="PLG42_07530"
     CDS             1944..4196
                     /locus_tag="PLG42_07530"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOU31097.1"
                     /translation="MKRSRKTLFFIFIALFAFSNFLKAQDDEILIKKINSAVTFDGRV
                     DDSEWNYLTQLQLVMHRPNFGNEPSEQSEVYIGYDERYLWIGARLFMKDPSKIFCTSK
                     RRDEMLYGMDAFGVLLDTYRDNENALAFFTAPSGLRTDFTISNDAAEMRGGGGGFGGG
                     VMNYSWNTFWDVKTSRDDKGWYAEMRIPFSSLRFKNINNLTRMGLSIVRNIGTNNEVD
                     TYPAIEPKYGMMAIYKPSLSKTIVLDGLKPAQPVYLSPYVIGGFTRDYSLNEENTEYL
                     KKDKPNFNAGFDIKYSIKSNFTIDLTANTDFAQVEADEQQVNLTRYSLFFPEKRMFFQ
                     ERSSLFNFSLGGMADNLFYSRNIGIAHGEMIRILGGARLTGHIGKWEMGLLDMQTEKF
                     EDVPSENFGVLRVRRQVFNPNSYIGSMMTSRIDLNGNKNIAYGLDGIFKVKGEDYLNL
                     KWAQTWDSQTGNSLNSINSSFIYANWERRSERGFAYNFGYTYSGLDFNPRAGFMRRGG
                     LQGYNGQLLYGWFPGEKSKIFSMAATIRGEYYTRIEDGKLENSIIEPGFRINTKKGFR
                     YDISMEYRTEGVQDDFEIADNIIISSGEYSFINFQGRFGTPSSRIFSVMGSIDAGQYY
                     DGYRLTIRPNINFNLSSSFRLMAGYQYNAIRFPDRSEHKKLDIHLINANLMYMLSTKL
                     SATLMVQYVNTSDDFIANFRLRYNPREGNDLYLVINDYQGFDRYGEIPHKPPFFNRTI
                     VVKYTHTFIL"
     gene            4396..>5493
                     /locus_tag="PLG42_07535"
     CDS             4396..>5493
                     /locus_tag="PLG42_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOU31098.1"
                     /translation="MKRISNSRRQFLRKATIASAAVTLAPRLLSCEADEPLKNKLPRW
                     KGFNLLDFFSANPPRNPQANSTTEDDLKWMRDWGFDFVRIPMAYPRYLKFDRTRDISK
                     DEICNYDPVVLEEVDRLIYLCHKYNLHVSLNLHRAPGYCINAGFHEPFNLWKDSDAQE
                     AFAAHWGMWAKRYKNISPEKLSFDLLNEPAYIEDMNDQFARKVALPGTTYRKVAEMAV
                     NAIRKESPERLIIADGNNVGNNVTPELFDLNIAQSCRGYYPHYVSHYQASWVWKDPSQ
                     APVPVWPGVIDGKEFGRKNLEELYKPWIDAVNKGIGVHCGECGCYNKTPHNVFLAWFE
                     DVLSILTGAGIGYALWNFRGDFGILDSRRSDV"
BASE COUNT         1719 a         1081 c         1200 g         1493 t
ORIGIN      
        1 gattaccgcc ttacaatcgc agaacttata ctcgacaatt ttactaaaaa atggactgaa
       61 tgggctcaca aaaatggtaa actcacccgc aaccaggcac acggttctcc gggaaatatt
      121 ctcgatcttt acgccgcagc cgatatacct gaaaccgagg gtacggaaat attcaggatg
      181 aaatttgcaa cctcggctgc taatgtaaca ggcaggaagt tagcttctgc cgaagctgcc
      241 acatggctcg atgaacactt ctcatccaca cttctggata taaaaaaagc agttgataag
      301 ttttttgttg caggcgtaaa ccatatcgtt taccatggta cctgcttctc cccgccagat
      361 gaaccctggc cgggtttcca tttttatgca gccgttgaac tgaagccatc aaatccgatc
      421 tggaccgatt ttcatgcact taatgaatat gtgacaaaag ttcagtcgtt tatgcaaagc
      481 ggcagacccg ataacgacct tcttctctat tttcctgcat ctgatactta tgcatcatat
      541 aataaaatga tgcttgagca ttttgacggc acaggacaaa gattccgcga tacatggttc
      601 agaaaagctg ccgaaaaaat gacaagcaga ggatatgcat acgacttcat ctccgaccag
      661 cagctgatga aatgcacaac agcaggaaaa gagatactca ccgaagggaa taacctgtat
      721 aaagcagtga ttgtacctca gtgcaaatat atcccggtgg aaacattcaa taaactgtgc
      781 aaccttgcag aagaaggagc agtggtaatc ttttatggca atctgcctga aaatgtttca
      841 ggatttgcag ggcttgaagt taaccgcgat atattcaatt atctgaaaga acagcttaaa
      901 ttctctgaaa cgaatcgtga aggagtcaga gtcgctgttt caggtacagg taaattgttt
      961 cagggagatt cactcgaaaa ccttctggtg agcgccggaa taagaagaga atcagtcacc
     1021 gatcagggcc tgcagttttt caggcgcaaa aacagtgaag gaactatcta cttcatcaat
     1081 aatccttctg ataaatattt tgaaggaaac atcacacttg aaactcctgc aaaatctgcc
     1141 ggtatattca atcctatgac aggtgaaaaa gggaaagtca aagtaaccgg gtcaaaagac
     1201 gggaaaataa aattatatct tcagcttaag ccttttgaat cgttaataat aaaggccttc
     1261 aaaactgcag tgcaggtccc tgattacctg ttttatgaaa agtcagggga tccggttgtt
     1321 attaacggta agtggaatct gagatttatt gaaggaggtc ccgtgcttcc ggcttctgtt
     1381 tccttaaatg agccggtgct atggactgct ttagagggtg aagaatataa agatttttca
     1441 ggctcagcag tttatagcac aactttcact gcacttgcct ttaaatcgaa atttctgaag
     1501 ctcaatcttg agaaagtatg cgagagcgca tgtataactc ttaacggaga atatattggc
     1561 actcttcttg ggcctgattt cagcgtggta attcctgctg aaaagctgaa gaagaccaat
     1621 aatattgaaa taagggttac gaaccttgct gccaacagga tcgcttatat ggacaggaaa
     1681 ggaattgaat ggaaaaagtt ttataataca aacttccctt caagacttcc gcaaaacagg
     1741 aaagaggggc tttttgatgc atctgcatgg caaccccgcc cctcaggatt acagggagag
     1801 gtttgtttaa tacccttaaa gccagtcaca aatagcagat gagttaatta ttaaagtttt
     1861 ttaacagaat caatcctgta attttcataa aattgcagga attttttatt gaatttgttt
     1921 gtctaaaaag caaaataatt taaatgaaaa gaagccgtaa aactttgttt tttatattta
     1981 ttgcactgtt tgctttttct aactttttga aagctcagga cgatgaaatt ttaataaaga
     2041 agattaattc tgctgtcact tttgatggtc gtgttgatga tagtgagtgg aattatctta
     2101 cgcaattgca actggttatg cacaggccca attttggcaa tgaaccttcg gagcagagtg
     2161 aagtatacat tggttatgat gagcgttacc tgtggattgg agcaaggcta tttatgaagg
     2221 atccttctaa aattttctgt acttcgaaac gaagggatga gatgctttac ggaatggatg
     2281 cattcggggt tcttttagat acttacaggg ataatgaaaa cgccttagcc ttttttactg
     2341 cgccgtcggg tctaagaacc gattttacta tttccaatga tgctgcagag atgagaggtg
     2401 gtggcggcgg cttcggaggt ggtgttatga actacagctg gaatacattc tgggatgtta
     2461 aaaccagcag agacgataaa ggctggtatg cagagatgag aatccccttc tcgagcctca
     2521 ggtttaaaaa cataaataac ctgacacgga tgggtctttc aattgtcaga aacataggaa
     2581 ccaataatga agttgatact taccctgcaa tagaaccaaa atatggcatg atggccatct
     2641 ataaaccttc tctgtcaaag acaatagttc tggatggtct taaaccggct caaccggtgt
     2701 atctttcacc ttacgtaata ggtggattta caagagatta ttcattgaat gaagaaaata
     2761 ctgaatattt aaaaaaagac aaaccaaact ttaacgcagg gtttgacatt aaatacagta
     2821 taaaaagcaa ttttaccatt gacctgactg caaacaccga ctttgctcag gttgaagccg
     2881 acgaacagca agtaaatctt acacgctaca gtcttttctt tccggaaaaa agaatgttct
     2941 tccaggaacg ttcaagcctt tttaacttca gtcttggagg aatggccgat aacctgtttt
     3001 acagccgaaa cattggaatt gcccatggtg aaatgatcag aattttaggt ggtgcaaggc
     3061 tgaccgggca tataggcaaa tgggaaatgg gactacttga tatgcaaaca gaaaaatttg
     3121 aagatgtacc atccgaaaat ttcggagtac tgagggtcag acgacaggta ttcaatccta
     3181 actcatacat cgggtcaatg atgacttcgc ggatagatct gaatggaaat aaaaatatcg
     3241 catacggact cgacggtata tttaaggtga aaggggagga ttaccttaat ctaaaatggg
     3301 cacagacatg ggacagccag acaggcaaca gccttaactc gataaattcc tcattcatat
     3361 atgccaactg ggaaaggcgt tcggaaaggg gatttgcata taattttggt tatacatatt
     3421 caggtctcga ctttaatcca cgtgccggat tcatgagaag aggaggtctt cagggttata
     3481 acggccaatt actctacgga tggttccccg gtgaaaaatc aaaaatattc agtatggctg
     3541 caacaataag gggagaatac tatacccgta ttgaagacgg aaagctcgaa aacagcatca
     3601 ttgaacctgg tttcagaatc aataccaaaa agggattccg ttatgatatc agcatggagt
     3661 acagaacaga aggggtccag gatgactttg aaattgccga taacataata atttcaagcg
     3721 gagaatattc cttcattaat ttccagggca ggttcggtac accttcctca aggatatttt
     3781 cggttatggg aagtatagat gccggtcagt actatgacgg ttacagactg acaattcggc
     3841 ccaatatcaa cttcaacctc tcttcaagct tcagacttat ggcaggatac cagtacaatg
     3901 cgatcagatt cccggataga agcgagcata aaaaactgga catccatctt atcaatgcca
     3961 acctgatgta catgctcagt accaaattgt cagcaacatt aatggttcag tatgtcaata
     4021 ccagcgatga ttttatagcc aacttcaggc tgagatataa cccaagggaa ggtaatgacc
     4081 tgtaccttgt tatcaacgac tatcagggct tcgaccgcta tggggaaata ccgcataaac
     4141 cacctttctt taaccgcaca atagtcgtta aatatacgca tacatttatt ctttgatttt
     4201 tgatggcaca cagatgacac tgatttgaca gattttcaca gatataatcc gtgattatcc
     4261 gtttaatcag tgctatcctt gtgccatttt taaaattcat atcatttcta ctggttgctg
     4321 gaggtataga aaaaataaat atatcggttt acacaataaa aattagaatt acttttgggt
     4381 gtaattcatt aaatcatgaa aaggatttct aactctcgtc gccagttttt aaggaaagca
     4441 actatcgcat cagctgctgt aacacttgcc ccccggctct tgtcatgtga ggcagatgag
     4501 cctttgaaaa acaagctgcc ccgctggaaa ggatttaacc tgcttgattt cttttcggca
     4561 aacccgccga ggaatccgca ggcaaacagt accacggagg atgaccttaa atggatgagg
     4621 gactggggtt ttgattttgt caggataccg atggcctatc cgcggtatct gaagttcgac
     4681 cgcacccgcg acatttcaaa ggatgaaatc tgtaattatg accccgtcgt actcgaagaa
     4741 gtcgacaggt tgatctattt atgccataaa tacaacctcc atgtaagtct taaccttcac
     4801 agggcacctg gttattgtat aaatgccggt tttcatgaac cctttaatct ttggaaagac
     4861 agcgatgcac aggaagcttt tgctgcacac tggggaatgt gggcaaagcg gtataaaaat
     4921 atttcacccg aaaaactgag cttcgacctt ttgaatgaac cggcttatat tgaagatatg
     4981 aacgaccagt ttgctcgtaa agtcgcacta cccggcacta catacaggaa ggttgctgag
     5041 atggctgtta atgcaattcg aaaagaaagc ccagaaagat tgattattgc cgatggcaat
     5101 aatgtaggga ataacgtaac tcccgaattg ttcgacctga atattgcaca gagctgcagg
     5161 gggtactatc cgcattatgt ttcgcactac caggcatcgt gggtctggaa agacccatca
     5221 caggcaccag tgcccgtatg gcccggcgta attgacggga aagaattcgg ccgcaaaaac
     5281 cttgaagagc tctataaacc atggatagat gctgtaaata agggtatagg agtacattgc
     5341 ggtgagtgtg gatgctataa taaaacaccc cataatgttt ttcttgcctg gttcgaagat
     5401 gtgctgagta tcctcaccgg ggcaggaata ggatacgctc tctggaactt taggggagat
     5461 ttcggaatac tcgactcacg caggagcgat gtt
//
LOCUS       DAOSNB010000172         5469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15038_length_5469_cov_3.883450, whole genome shotgun sequence.
ACCESSION   DAOSNB010000172 DAOSNB010000000
VERSION     DAOSNB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_5469_cov_3.883450"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLG42_07540"
     CDS             <1..706
                     /locus_tag="PLG42_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31099.1"
                     /translation="MLMRRLKNWKIPFGDAVINKLLSHYQLKSAQDLYYQIALEKINL
                     LDIKDLLTKPEVKDKAEKDNVETDSSTVIEDRLSQLYTDYLVIDEKIEGLDYKLAKCC
                     NPVFGDRIFGFVTVSDGIKIHRTSCPNAQYMLLRYPYRYVSARWTKTSDMPDFRASVK
                     VTGIAEVGIINRIADVITENRIVLRSFNYKMNEGMFEGMLQIMVPNNDVLQSIIKKIL
                     SIKGVLKAVRTESGEW"
     gene            complement(722..3661)
                     /gene="dnaE"
                     /locus_tag="PLG42_07545"
     CDS             complement(722..3661)
                     /gene="dnaE"
                     /locus_tag="PLG42_07545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOU31100.1"
                     /translation="MFLNCHSYYSLRYGTMSVEQLVSKAAEYRAEAIALTDINNSTGV
                     LEFVKLCREKNIKPLAGIEFRNGNEFLYTGIAMNNEGFRELNEFLTSHNINGMPIEFP
                     APRFNNACIIYSVGKIPGRKLYENEYIGIRPKEVNRLISFPPALKRSKMLIINPVTFA
                     ASDDYQIHKSLRAIDNNILLSHLQPSQCAPNDEIFRTKDEIINIFKSFPELIETTEKL
                     ISNSDFSPDLESNKNKKVYSASRYDDKLLLEKLAWDGMLYRYGNNNEEAKRRIKHELE
                     IIDKLGFSAYFLITWDIVRYSMSRGFYHVGRGSGANSIVAYCLKITNVDPIDLNLYFE
                     RFINPRRSSPPDFDIDYSWKERDEVLDYIFKRYGYRHTALIGTINTFRGKSIVRELGK
                     IYGLPKEEIDNLIDNPCADINRNGIADKIFETGLKIADFPNIRSIHAGGVIISEEPVT
                     YYTALDFPPKGFQTTQWDMYTAEEIGFEKLDILSQRGIGHIRESAEIILKNRNIKVDI
                     YDIDKFKQDPLVKEFLRKGETKGCFYIESPAMRGLLKKLKCKDYKTLVAASSVIRPGV
                     ARSGMMREYIYRFHNPGKFEYLHPVMKEQLEETFGVMIYQEDVIKICHHFAGLDLADA
                     DILRRAMSGKYRSKQEMKRIMNKFFSNCREKGYNEEITKEVWRQIESFAGYSFSKAHS
                     ASYAVESLQSLYLKAHFPLEFMVAVINNFGGFYSAWVYVHEAKRYGGKINLPCVNKST
                     YKTTIYGKDIYLGFIHIQNLEEKIARLIEEERQTGGPFNSLNDFINRVNPGLEQLILL
                     IRAGAFRFTGKPKASLLWEAHMLMNKSKPEPTKPLFFSPARMYSLPELEYDKLSDAYD
                     EIELIGFPVSVSWFDMLETSFRGEIFAHELNKYEGKKVKMVGQLVTVKYIKTVKNEWM
                     NFGCFIDAEGNFFDTVHFPQSLKNYPFKGSGIYLIAGKVVVDFGFPSIEVEKMAKLPV
                     KKDPRSV"
     gene            complement(3732..4985)
                     /gene="dinB"
                     /locus_tag="PLG42_07550"
     CDS             complement(3732..4985)
                     /gene="dinB"
                     /locus_tag="PLG42_07550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOU31101.1"
                     /translation="MIAGNTERAVLHLDLDTFFVSVERLRNSRLNGKPVIVGSLSDRG
                     VVASCSYEARQYGVSSAMPMKLAMNLCPDAIVIRGDMELYSKYSNIVTEIIAERAPLY
                     EKASIDEHYIDLTGMDRFFGCSRWAHELRRYIIKETGLPVSMGLSVNKTVSKIAADEA
                     KPNGEKEVAKDIVIPFLDPLSIKKIPMIGQKTYQILRAMGISTIYTLRNIPPEMLERL
                     MGKNGIEIWKRANGIDPTPVISYSEQKSISTEHTFEKDTTDVKVLNQTLVSMVEKIAF
                     EMRSQEKLTSCVTVKIRYSNFDTYTIQKRIPYTAFDHILISAAKELFERLWQRRMLIR
                     LIGVRFSHLVRGVQQLDMFDDTPEKVNLYMAMDRLRKRFGRYAVQRASGVMTVQEREE
                     KALKRLENALNEHKMMEERLKRYWW"
     gene            5089..>5469
                     /locus_tag="PLG42_07555"
     CDS             5089..>5469
                     /locus_tag="PLG42_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOU31102.1"
                     /translation="MYFASNIKILRKRKGRTQDEVACALNLKRSTLSGYENGIAQPDI
                     ETLISFSSYFNVSIDTLLRVDISKLSDSQLGELERGYDAYIRGSNLRVLATTVDRSNK
                     ENIELVNEKAKAGYTTGYADPEYIG"
BASE COUNT         1454 a         1082 c         1153 g         1780 t
ORIGIN      
        1 gatgcttatg cgccgcctta agaactggaa aatacctttc ggagacgcag tgataaataa
       61 actcctttcg cactaccagt taaaatcggc acaggacctt tattatcaaa ttgctcttga
      121 gaaaattaat ctactggata taaaagattt actaacaaaa cctgaagtaa aagataaggc
      181 agagaaagat aatgttgaaa cagatagcag caccgttatt gaagaccgtt tatcgcaatt
      241 atatacagat taccttgtta ttgatgaaaa aatagaagga cttgattata agcttgcgaa
      301 atgttgcaat ccagtttttg gtgacaggat ttttgggttt gttacagttt cagatggcat
      361 aaaaatccac agaacttcat gccctaatgc ccagtatatg ttattgagat atccatatag
      421 atacgtatca gccaggtgga ccaaaacaag cgatatgcct gatttcaggg catcggtaaa
      481 agttacaggc attgcagaag ttgggattat aaacaggatt gctgatgtaa ttactgaaaa
      541 cagaattgta ttgcgaagtt ttaattataa aatgaatgaa ggaatgtttg aaggtatgct
      601 tcagataatg gtgcctaata atgatgtatt acaaagtatt ataaaaaaga ttttgtcaat
      661 taaaggagtt ctgaaggctg taaggactga gagcggagag tggtaaaaac attattaaaa
      721 atcaaaccga acgtggatct ttttttaccg gcagttttgc cattttctca acttctatgg
      781 aggggaaacc aaaatccaca acaaccttgc ctgctattag atatatgcca cttcctttga
      841 aaggataatt tttcagtgat tgtggaaaat gcactgtgtc aaaaaagttt ccttctgcat
      901 caataaagca tccgaagttc atccattcgt tttttactgt tttaatgtat tttacggtaa
      961 caagttgtcc taccattttt acttttttcc cttcatattt atttaattca tgcgcaaata
     1021 tttctccccg gaatgatgtt tccagcatat cgaaccagga aacagatacg gggaatccta
     1081 taagttcgat ttcgtcgtaa gcatcggaaa gcttgtcata ttcaagttcg gggagtgaat
     1141 acatccttgc aggagagaag aagagcggct tagtaggttc cggcttactc ttattcatca
     1201 gcatgtgtgc ttcccacaga agtgaggctt tcggttttcc tgtaaacctg aaggcgccgg
     1261 cccgtataag aagaataagc tgttcaagac cggggttaac acggttaata aagtcgttca
     1321 gactgttaaa tggaccgccc gtttgtctct cttcttcgat cagtctggca attttttctt
     1381 caaggttctg tatatgaata aaacccagat atatatcctt tccataaata gttgtcttat
     1441 aggtactttt gtttacacag ggcagattaa ttttacctcc gtatcttttt gcttcatgga
     1501 cataaaccca ggcagagtaa aaccctccga aattattaat aacagctacc ataaattcaa
     1561 gcgggaaatg agctttcagg taaaggctct ggaggctctc aacggcgtat gatgcagagt
     1621 gtgccttgga aaaggaatat ccggcaaatg actctatctg gcgccagacc tctttggtaa
     1681 tttcttcatt gtaacctttt tccctgcagt ttgagaaaaa cttgttcatt attctcttca
     1741 tttcctgttt cgaccggtat tttccgctca ttgcgcgcct gagaatgtct gcatcggcca
     1801 gatccagacc tgcgaaatgg tggcatattt taattacatc ttcctgataa atcattacac
     1861 cgaatgtctc ttccagttgt tctttcataa caggatggag atattcaaac ttacccgggt
     1921 tatgaaaacg gtaaatatat tctctcatca tgccagagcg tgcaacacca ggtcttatca
     1981 cagagctggc agcaacaaga gttttgtaat ctttacattt tagttttttg agaagtcccc
     2041 gcattgccgg actttcaata taaaagcatc ctttggtttc accttttctt aggaattctt
     2101 tcacaagggg gtcttgtttg aatttatcga tgtcgtatat atccacttta atgtttctgt
     2161 ttttaaggat tatttcggcg ctttcgcgga tatgcccaat acctctctgg cttagtatgt
     2221 cgagcttttc aaagcctatc tcttcagcag tgtacatgtc ccactgtgtg gtttgaaagc
     2281 ctttgggcgg aaaatcaagt gctgtataat aggtaaccgg ctcttcagag attataacgc
     2341 ctccggcatg aatgcttctg atgttgggga agtcggcaat tttaagtcct gtttcgaaaa
     2401 tcttatcggc tattccgttg cggttaatat cggcacaggg gttatctatc aggttatcaa
     2461 tttcttcctt gggtaggcca tatattttcc ccagttctct tacaatagat ttacccctga
     2521 aagtatttat tgtgcctatg agtgcggtat ggcggtatcc gtatcttttg aaaatgtagt
     2581 cgagcacttc atctctttct ttccatgagt aatcaatatc gaagtcgggc gggctgcttc
     2641 tgcgggggtt gatgaaccgt tcaaagtaaa ggttcaggtc gatagggtca acgttggtga
     2701 ttttcagaca ataagcaact attgaattgg caccgctacc tctgccgacg tggtaaaagc
     2761 ctctcgacat tgagtaccgt acgatatccc aggtaatcag gaaataggca gaaaagccga
     2821 gtttgtcgat tatttcaagc tcgtgtttaa ttctccgctt tgcttcttcg ttattgttgc
     2881 cgtacctgta aagcatgccg tcccatgcaa gtttctcaag tagcagtttg tcgtcgtaac
     2941 ggcttgctga gtacactttt ttatttttgt tgctttcaag gtcgggtgag aaatcagagt
     3001 tactgatcag tttttcagta gtttcaatca gttcggggaa ggatttgaag atgtttatta
     3061 tttcatcctt tgtgcggaat atttcatcgt tgggggcgca ttgtgagggc tggagatggc
     3121 tcaggagtat gttattgtcg atggccctca ggcttttgtg gatctggtaa tcatcacttg
     3181 cagcaaaagt tacaggattg attattaaca ttttcgagcg tttaagggca ggggggaagc
     3241 tgataaggcg gtttacttct tttggccgta tgcctatgta ctcattttcg taaagtttgc
     3301 gtcccggaat tttaccgact gaataaatta tgcaggcatt attgaatcgt ggcgcaggga
     3361 attcgattgg catgccattg atattatggc tggtcaggaa ttcattcagt tcccggaaac
     3421 cttcattatt cattgcgatg ccagtgtaca ggaactcgtt tccgtttctg aactcaatgc
     3481 cagcaagagg ctttatgttt ttttctctgc agagtttaac gaattcaagt acgcctgtgg
     3541 agttgtttat atcggtaagg gcaattgcct ctgcacggta ttcagcagct ttgcttacaa
     3601 gttgttcgac agacattgtt ccgtagcgca ggctgtaata tgaatggcaa ttcagaaaca
     3661 ttgtttttgt cttttgttac ttagccagca tacagcatac aaggtactgt gctgtttttt
     3721 gtgtttattt ttcaccacca gtaccttttc agtctttcct ccatcatttt atgctcgtta
     3781 agggcatttt caagccgttt taaggctttt tcctctcttt cctgtactgt cataacccct
     3841 gatgctcttt gaacggcata tcgcccgaaa cgctttctaa gcctgtccat tgccatgtac
     3901 agatttactt tttcaggggt atcgtcgaac atgtcgagtt gttgaacgcc ccttacaagg
     3961 tggctgaacc gtaccccgat aagtctgatg agcatacggc gctgccagag tctttcaaaa
     4021 agttcttttg cagcgcttat caggatatgg tcgaatgcgg tgtaggggat acgtttctga
     4081 attgtataag tatcgaagtt ggaatatctg atttttactg ttacacacga agtgagtttt
     4141 tcctggcttc tcatctcaaa tgctattttt tcgaccatgc ttacaagagt ctggttgagc
     4201 actttcacgt cggtagtatc tttttcgaat gtatgttctg tgcttattga tttctgttct
     4261 gaatagctta tgacaggggt ggggtctatt ccgtttgccc ttttccagat ttcaataccg
     4321 ttttttccca tcaatctttc gagcatttcg ggagggatat ttctcagtgt gtatattgtt
     4381 gatattccca tagctctcag tatctggtat gttttctgtc ctatcatagg tatttttttg
     4441 attgagagcg gatcgaggaa agggataact atgtcttttg ccacttcttt ttcgccgttt
     4501 ggtttagctt catcggcagc tatttttgaa acggttttgt tgacagaaag tcccattgat
     4561 acaggcaggc ctgtttcttt tattatatat ctgcgaagtt catgagccca ccggctgcat
     4621 ccgaaaaacc tgtccattcc ggtaagatct atataatgtt catcaatcga agctttttca
     4681 taaagaggcg ccctttcggc aattatttcc gtaactatat ttgagtactt actgtaaagc
     4741 tccatatcgc cgcgtatgac tatcgcgtcg gggcaaagat tcatagcaag cttcattggc
     4801 atggctgagc tcacaccata ttgtcttgct tcataactgc agcttgccac aactccacgg
     4861 tctgacaggc ttccgactat caccggtttg ccgttcagcc tgctgttcct caacctctcc
     4921 accgaaacaa aaaaggtgtc gaggtcaagg tgcaaaaccg ctctctctgt attgccagca
     4981 atcatggcat tcatttttta aaaaattatt tggttatatc aaaaatctat tttaattttg
     5041 attaaaaatt aatcaaaatt ctgactataa tataatcaaa tttttgatat gtacttcgcc
     5101 agcaacataa aaattttaag aaagaggaag gggaggaccc aggatgaagt tgcctgtgcc
     5161 cttaacctta agcgctcaac actgagcggg tatgagaatg gcattgccca gcctgatatt
     5221 gaaacactga tttcattttc aagctatttc aatgtttcaa tagacacact tctccgagtt
     5281 gatatttcga agctttccga cagtcagctc ggtgagcttg aacggggtta cgatgcctat
     5341 atcaggggaa gcaatctcag agtgcttgcc acaactgtcg acagaagcaa taaggaaaac
     5401 attgaactgg taaacgaaaa agcgaaggct ggctatacaa caggttatgc agatcctgaa
     5461 tatatagga
//
LOCUS       DAOSNB010000173         5450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15094_length_5450_cov_5.066914, whole genome shotgun sequence.
ACCESSION   DAOSNB010000173 DAOSNB010000000
VERSION     DAOSNB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15094_length_5450_cov_5.066914"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PLG42_07560"
     CDS             complement(<1..1217)
                     /locus_tag="PLG42_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOU31103.1"
                     /translation="MRKRDFIKRAGILGMGAIMSNKIFIEMIDKYADVPAGELARNED
                     FWLEVRKGYRLNPDIINLENGYYNIIPEQTLERFIRHVRDVNYQGSYYMRTVQFDNKK
                     AMAAKLAAIAGCQADELIITRNTTESLDIIISGYPWKEGDEAVMAEQDYPTMLTMFRQ
                     VANRYGVVNKVVSVPHDPGSDDEIVNLYANAITDKTRLLMICHMINITGHILPVRKIC
                     DMAHSRGVEVMVDGAHTFGHIRFTIPELGCDYFGSSLHKWMSVPLGAGLLWVKREHIS
                     KIWPLMAPVEDKPDDISRLNHTGTHPVHTDLTVEDAIEFYLMLGPERKEARLRYLQHY
                     WSDKVRVLPHIEMYTPSDPARSCGIATVGVRGMKPAELADILFKKYRIYTAPIDGAGV
                     HGCRITPNVFTTT"
     gene            complement(1375..2568)
                     /locus_tag="PLG42_07565"
     CDS             complement(1375..2568)
                     /locus_tag="PLG42_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31104.1"
                     /translation="MAKLAMLGAGFIGDFYTHSLHGLRSRDRVVIVGDRDESRAKVFA
                     IKHGIPRWATSLKEAVNDPEAELVVVGLPNNLHLEAVKLAAEAGKGVLCTKPLGRNAA
                     EAYEMLEAVEKAGVFHGYLEDLVYTPKTLKGLEAVRQGSLGQIIWVRSRETHPGPHSD
                     WFWTKEISGGGVLLDMGCHCIEIARNYIGKDIRPVEVISTADTLVKPIDAEDNSLGWV
                     RYENGAIGQFEVSWSFRGGMDLRDEVMGTEGTIWVNNFLRTGIEIFTAGGKKGYVAEK
                     AESEKGWIFPVGDEVTELGYNHMFADMFRAFENKKEPAETFYDGYIVNEIMDACYRSA
                     KSKKWEPVNLKVWRGQEKAKPIAEIREYDRDHIFVKEEILPDGTVKIILKEKATGKIV
                     ERIKK"
     gene            2770..4368
                     /locus_tag="PLG42_07570"
     CDS             2770..4368
                     /locus_tag="PLG42_07570"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOU31105.1"
                     /translation="MSSTEKFSASINEGYLCKGESIVLGTAMLNGEVVQGAHVRIPLK
                     TMNRHGLIAGATGTGKTKTLQVIAEQLSEKGVPVLLMDVKGDLSGIAMPGEEKPFITE
                     RHSKLGIPWSPQGYPVELLTLSQQDGVRLRATVSEFGPVLLSRILNLNDTQAGVMAVI
                     FKYCDDNGLALLDLKDLKQVIQFMTGEGKDEVEKAYGRISTSTTGIILRKILELEQQG
                     AELFFGELSFDIRDLLRRDKNGYGYINIVRVTDIQDRPKLFSTFMLSLLAEIYNTMPE
                     IGDAEKPELVMFIDEAHLIFNQASKELLDQIENIVKLIRSKGIGVFFVTQNPTDIPQD
                     VLAQLGLKIQHALRAFTAADRKAIKLTAENYPVSEFYKTDEVIMSLGIGEALVTALNE
                     KGIPTPLAAVMLRAPRSRMDILTPSEISAINRSSELVRKYFQVIDRESAYEILGRKVA
                     MLEKERADEEARRAWEKQREAEWKAQRQREVDWSRGAGRTSRQYSGRRTYSSRKRSTV
                     PPAVKVLTSATFIRGVFGILKKMI"
     gene            complement(4403..5374)
                     /locus_tag="PLG42_07575"
     CDS             complement(4403..5374)
                     /locus_tag="PLG42_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOU31106.1"
                     /translation="MTHYKELGLVNSRELFKKAMAGGYAIPAFNFNNLEQLQAIISAC
                     VETKSPVILQVSKGARKYANQTLLQYLAKGAVEYAKELGYAIPIVLHLDHGDSFELCK
                     SCIETGFSSVMIDGSHLPYEENVKLTRQVVDFAHQYDVTVEGELGVLAGIEDEVSSEH
                     THYTRPEEVEDFVSRTGVDSLAISIGTSHGAYKFKLKPGETVPPLRFDILEEVEKRLP
                     GFPIVLHGASSVPQEIVDEINRYGGKLENTAGVPEEQLRRAASSAVCKINIDSDGRLA
                     VTAAIRKVFYEKPSEFDPRNYLGPAREKLKELYKHKIINVLGSAGKA"
BASE COUNT         1505 a         1282 c         1127 g         1536 t
ORIGIN      
        1 tcagttgtcg taaacacatt tggtgttatg cggcagccat gaactccggc tccatctatt
       61 ggggctgtat atattctata ctttttgaaa aggatatcag ccagttcagc cggtttcatg
      121 cccctgactc cgacagtcgc aataccgcac gaacgagcag ggtcagaagg tgtgtacatt
      181 tcaatatgtg gcaaaaccct tactttgtcg ctccagtaat gctgaaggta cctcagccgg
      241 gcctccttac gctccggtcc aagcataaga taaaattcta tagcgtcctc aacagtcagg
      301 tcggtgtgaa caggatgggt gccagtatgg ttcagccttg aaatatcatc cggcttgtct
      361 tcaacaggcg ccatcagggg ccatattttg ctgatatgct cccttttcac ccagagtagt
      421 cctgctccaa gcggcacact catccacttg tgcaggctgc tgccgaaata gtcacatccg
      481 agctcaggga tcgtaaacct tatgtgcccg aaagtgtgtg caccatcaac catcacctca
      541 acaccccggc tgtgtgccat atcgcatatc tttcttacag gcagtatatg accggtaatg
      601 ttgatcatat ggcatatcat caatagcctt gttttatcgg ttatagcatt tgcataaaga
      661 ttcacaattt catcatccga ccccgggtca tgcggtacag agacaacttt attaaccact
      721 ccatatcggt tagcaacctg cctgaacatg gtaagcattg tgggataatc ctgctcggcc
      781 attacggctt catcgccttc tttccatgga taaccactaa taatgatatc aagcgattcg
      841 gtagtgttac gtgttatgat aagttcatca gcctgacagc cggctatcgc agcaagcttt
      901 gccgccatcg ccttcttgtt gtcgaactga acagtccgca tataatatga gccctggtaa
      961 tttacatccc gaacatgcct tataaacctt tcaagagtct gctctggtat tatattgtaa
     1021 tatccgtttt caaggttgat gatatcagga ttaagccggt aacctttccg aacctcaagc
     1081 cagaaatctt cattgcgtgc cagttcaccg gcaggcacat cagcatattt atcaatcatt
     1141 tcaataaaaa ttttgttcga cataatagca cccattccca gaatacccgc tctttttatg
     1201 aaatcccttt tgcgcatgaa tttatttctc aataaaaaaa atttattcct atatgttctt
     1261 ttgggcaaat cttaatttac tttatggaaa agggatgtta cttactacaa agaaccctga
     1321 ggagttcgac aaagaagtct gtaataaatt atttttctgc cagtttcgat tcgcttattt
     1381 ttttattcgc tccacaattt tgcccgttgc cttttcctta aggataatct ttaccgtacc
     1441 gtcgggcaat atctcttcct tgacaaaaat atgatctcta tcgtattctc ttatttcagc
     1501 gattggtttg gccttttcct gtcccctcca cactttcaga tttacaggct cccatttttt
     1561 cgattttgcc gagcggtagc aagcatccat aatttcgttc actatatatc cgtcgtaaaa
     1621 agtctccgca ggctcttttt tattctcaaa agccctgaac atatcagcaa acatgtggtt
     1681 atagccgagt tctgtcactt catcgcctac cgggaatatc catccttttt ccgattctgc
     1741 tttttcagca acatatcctt ttttgcctcc tgccgtgaag atttcgattc ctgtgcgcag
     1801 gaagttattc acccatattg ttccctcggt tcccataacc tcatcgcgaa ggtccatccc
     1861 gccccggaag ctccagctta cttcaaactg tccgatggca ccgttttcgt aacgtaccca
     1921 gccgagtgag ttatcttctg cgtctatcgg ttttacaaga gtatcggctg tactgataac
     1981 ctctacaggc ctgatatcct tgccgatgta attgcgtgct atctcgatgc agtggcatcc
     2041 catgtcgaga agtacgccac ctcctgagat ctctttggtc cagaaccagt cgctgtgtgg
     2101 gcccgggtgt gtctctcgag agcgcaccca gataatttgc ccgagtgagc cctgcctcac
     2161 agcttccagc cctttcagag tcttaggagt gtaaacaagg tcttcaaggt aaccatgaaa
     2221 gacccctgcc ttttcaactg cttcaagcat ttcataagct tctgctgcgt tgcgtcccag
     2281 aggcttggta cacagcacac ccttaccagc ctcagcagca agctttactg cctccaggtg
     2341 aagattgttt ggcagaccaa ccacaacaag ctcagcctcc ggatcattta ccgcttcttt
     2401 aagtgaagtg gcccatcgcg gaatgccatg cttaatcgca aaaacttttg ccctcgactc
     2461 atcacggtcg cctacaatca caacacggtc gcgactgcgt aagccatgaa gggaatgggt
     2521 ataaaaatcg ccgataaaac cggcaccaag cattgcaagt tttgccataa ctttcttttt
     2581 taacagtttc tttaattatc aaaattaaaa aattggcata aaaaatcctt aacggaacaa
     2641 ttaaatcaat aagtaacgca taaggcttaa tgtaattaaa taagtatctt attacgctaa
     2701 aaactaaaaa tagttttctg attttaatta taactggtaa cttgcgtacg gaataaaaaa
     2761 tcatttccta tgagttctac agaaaaattt tcagcatcaa taaatgaggg gtatttatgt
     2821 aaaggtgaaa gcatagttct tggaacagct atgctgaacg gcgaagtggt tcagggggca
     2881 catgtcagga tcccccttaa aaccatgaac cgtcatggtc tgattgccgg agctaccggc
     2941 acaggaaaga ctaaaaccct tcaggtgatt gccgaacagt tatcggaaaa aggtgttcct
     3001 gtacttctaa tggatgtaaa gggtgatctt agcggaatag caatgcccgg agaagaaaaa
     3061 ccattcataa ctgaacgtca ctccaaatta ggcattccat ggtcacctca gggctatcct
     3121 gtagagcttc ttaccctttc gcagcaggat ggcgtgcggt taagggcaac cgtatcagaa
     3181 ttcggaccgg tgctgttatc acgaatactc aaccttaacg acacccaggc aggtgtgatg
     3241 gctgtcatat tcaaatactg cgacgataac ggactggcac tactcgacct caaagatctg
     3301 aagcaagtta ttcagttcat gacaggcgaa ggtaaggatg aagttgaaaa agcttacgga
     3361 agaatttcga cttctacaac agggataata ctaaggaaga tacttgaact tgagcaacag
     3421 ggtgcagagc tgtttttcgg agagctttca ttcgatataa gagatctgtt gcgccgtgac
     3481 aaaaatggct acgggtatat taacatagtg agagtaacag atatacagga cagacccaag
     3541 ctcttctcca cttttatgct gtcgctcctt gctgaaattt ataatacaat gcctgaaata
     3601 ggtgatgctg aaaaacctga acttgtaatg ttcattgatg aagctcatct gatattcaat
     3661 caggcaagca aagaattact tgaccagatt gaaaacatag taaaactgat aaggtcgaaa
     3721 ggcataggtg tctttttcgt aacgcagaac ccgactgata taccccagga tgttcttgcc
     3781 cagctcgggc ttaagataca gcatgcattg agggcattta cagctgccga ccgtaaggct
     3841 ataaaactca cggcagaaaa ttatcctgta tcagaatttt ataaaaccga cgaagtaata
     3901 atgtctcttg gtattggtga agcactcgtt actgctttga atgaaaaagg tattcccaca
     3961 ccgcttgcag ctgttatgct tagagctccg cgcagcagga tggatattct gactccttca
     4021 gagatatcag caatcaacag gtcatctgaa cttgtaagaa aatattttca ggtgattgac
     4081 cgtgaaagcg cctacgaaat tcttggaagg aaagtcgcga tgcttgagaa ggagcgtgcc
     4141 gatgaggagg caaggagagc atgggaaaag cagagggagg cagagtggaa ggctcagaga
     4201 cagagagagg ttgattggtc gcgcggagct ggccggactt caagacagta ttctggcaga
     4261 agaacctatt cttcaaggaa aagaagcacg gttccgccag cagtgaaagt tctcaccagc
     4321 gctacattta tacgcggtgt atttggaata ctgaaaaaaa tgatttagaa tcttccccct
     4381 tcatgatgaa ggttgagagg gatcacgctt ttcctgcgct acccagaacg tttattatct
     4441 tatgcttgta gagttccttc agtttttcac gtgccggacc aaggtaattt ctcggatcaa
     4501 attctgatgg cttctcgtaa aaaactttac gtattgctgc agtaacagca aggcgtccgt
     4561 cagagtcgat gtttatcttg catactgccg atgatgctgc cctgcgcaac tgttcctcgg
     4621 gcactcctgc agtattctcc agtttgcctc cgtaacggtt aatctcatca actatttcct
     4681 gaggaaccga agatgcaccg tgcaaaacaa tcggaaaacc gggtagcctt ttctcaactt
     4741 cttcaagaat gtcaaaacgc aaaggaggaa ccgtctcccc gggctttaac ttgaacttgt
     4801 aagctccatg tgaagtacca attgatattg ccagcgaatc aacaccggta cggcttacaa
     4861 aatcttccac ctcctccggg cgtgtataat gcgtgtgttc tgacgacacc tcatcttcta
     4921 tacctgccag cacacccagt tcgccctcaa ctgtaacatc atactggtgg gcaaaatcga
     4981 caacctgcct ggtaagttta acgttctctt cataaggcaa atgcgaaccg tcaatcatta
     5041 ctgaagaaaa acctgtctca atacacgatt tgcacagttc aaaagagtca ccatggtcga
     5101 gatgaagaac tatagggatt gcatatccaa gttccttagc atactctact gcacccttgg
     5161 caagatactg cagaagcgtc tgattggcat atttcctcgc tcccttcgat acctgaagga
     5221 taacgggtga tttcgtttcc acacaggcag atataatagc ctgaagctgt tcgagattgt
     5281 tgaaattaaa cgccggaatg gcatatcctc ctgccattgc ctttttaaat agttctcttg
     5341 aattaaccag tcctaattcc ttataatgtg tcatggtact ttattgtttt aaaaacggag
     5401 tgcaagttac ctatttagtt tattgcttca aagtttttaa cgatttttat
//
LOCUS       DAOSNB010000174         5431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15164_length_5431_cov_4.143601, whole genome shotgun sequence.
ACCESSION   DAOSNB010000174 DAOSNB010000000
VERSION     DAOSNB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15164_length_5431_cov_4.143601"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..563)
                     /locus_tag="PLG42_07580"
     CDS             complement(123..563)
                     /locus_tag="PLG42_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU31107.1"
                     /translation="MSSKAKILVVEDDADLVAAIVKMLEVKGYEVACAYDPQEGWEKL
                     KIHVPDLIILDVMFGAKGESKGFDFAQKVRLDRQYSHIPILMITAINAQKPYFNFSPE
                     TDAEFLPVDSFIEKPVRSEELFLKVEELLQQKVSKWHKLPEKNS"
     gene            complement(752..1225)
                     /locus_tag="PLG42_07585"
     CDS             complement(752..1225)
                     /locus_tag="PLG42_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOU31108.1"
                     /translation="MVKNKKTLVLGIGNTILSDDGIGPKIIRDLSLVFDDNNISFKEA
                     CCGGIEIAELISGYDCVVFIDAIHTGEGKPGDVYFFDISDFRETIHLSNVHDLSFITA
                     IELAKSLGMDLPASIIIVGVEILTDSEFGEELSPPLKERYETILEEVKTFVKGLV"
     gene            complement(1608..3188)
                     /locus_tag="PLG42_07590"
     CDS             complement(1608..3188)
                     /locus_tag="PLG42_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31109.1"
                     /translation="MDDKLDNSVNFHKHSSLRCCCGNTDMGHQGISRRKFVQMTGAGT
                     LGIITIQRLTWDMLANERPVDEYLPARQPLVVKPVLTYEVPERREKTSWRAWGGIQTE
                     NDASAEIARINSELKKLKSDADFPVDFLPVSAVRSRQDLAGITDIDRADLILIYAAGG
                     GMNIFAELNSRGKDIIFFCRHRSGPVYLWYEIISPRYLRQHTDELKVKGVDDSDVVID
                     SQDELLWRMRALCGLKNTRGSRILAIGGFGAWSQPLETTMKMVKEHYNFEIRDISYEN
                     LGNLIKEAREDRALVKQAKSSSDRYLADTGIKLETERKYVENCFVLGEVFRRLMEREG
                     CRAITVNNCMGTIMPIADTTACLTLSLLNDAGFLAFCESDFVVIPAGILLANISGKPS
                     FLNDPTYPHDWLITLAHCTAPRRMNGITLEPARIMTHFESDFGAAPKVEMKKGQVVTN
                     VIPDFEFRKYIGLPGKIVENPFMDICRSQIEVSFSCESEKVAKNMRGFHWLTVYGDYL
                     KETGYALKKIGIEFEILT"
     gene            3489..4799
                     /locus_tag="PLG42_07595"
     CDS             3489..4799
                     /locus_tag="PLG42_07595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU31110.1"
                     /translation="MALFEIKMPKLGESITQATIIRWFVKEGDYVKEDDVLLDIATDK
                     VDSEIPSPVAGTVKKIFYQENAIVQVGEVIAIIDTEANSSGKNKNIDEKNSGLQQTEK
                     NQLEEKNLNTSDEIIKPDKKEASARFYSPLAKLIARRENISAEELEKIPGSGINGRVQ
                     KKDILAYLEKRKVASATSASVVNDHTPVKSEALKYSMPVYQEDEIIEMDRIRKITAEH
                     MIRSKQTSAHVTNILEADVTAIVNWRNKIKDEFEKREGIKLTYLPLIIEAVIKSLKEF
                     PMLNSSVDGNRIILKKRINIGIAVALPDSNLIVPVIKNADYLNITGLAKEINRLAENA
                     RKNDLLPDDVNDGTFTVTNFGTFRNTIGTPIINQPQVAILGIGTVEKKPSVLETPDGD
                     VIAIRHKMFLSLTYDHRIINGAYAGSYLRRLADYLETFDTERTI"
     gene            4828..>5431
                     /locus_tag="PLG42_07600"
     CDS             4828..>5431
                     /locus_tag="PLG42_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOU31111.1"
                     /translation="MSANKKYHIKTTDKSVLERWYRLMTLGRALDERAPNYLKQSIGW
                     SYHAPYAGHDGIQLAIGQVFEKNKDFLFPYYRDMLTNLSAGLTATEIILNGISKATDP
                     ASGGRHMSNHFAKPEWNIQNVSSCTGNHALHAVGVARAIRKYRADGVAICSMGESAVS
                     EGYVYEAINGASREQLPVIFVIQDNGYGISVPKSDQTANRK"
BASE COUNT         1725 a         1112 c          963 g         1631 t
ORIGIN      
        1 gtaatgtaaa gatattgctt aggtgaagaa ttgccagaga atattcgctg aatatagtac
       61 ggaattatca gattattaat aaaatattta tcttcaagca ggtaatataa ccagaattaa
      121 aatcaactat ttttttcagg caatttatgc cacttactaa ctttctgctg cagaagttct
      181 tcgactttaa gaaacaattc ctcagatctg accggcttct caataaagga atccaccgga
      241 agaaactcag catctgtttc gggtgaaaag ttaaaataag gcttctgagc attaattgcg
      301 gtgatcatca gaatgggaat gtgcgagtat tgcctgtcaa gccttacttt ctgtgcaaaa
      361 tcaaagcctt ttgattcgcc tttagctcca aacattacat caagaatgat caggtcaggc
      421 acatgaatct tcagtttttc ccatccttcc tgaggatcat atgcacatgc aacttcataa
      481 ccttttactt ccagcatttt aactattgca gctacaaggt ctgcatcgtc ctcgacaacc
      541 agaatttttg cttttgaact cattatttat tccctccctg attattaatc cgtattgatg
      601 aaaaattttt aacttattct aaataagatc agtctaaaaa ccatatcttt tatctgtaat
      661 aaaattataa aaatattacc gaacaataga tataaggctt taaaattttt acagatcttc
      721 ctttttcacc gatactatgt tgttctcaag attacaccaa tccttttaca aaggttttaa
      781 cctcttcaag tattgtttca tatctttctt ttaatggtgg tgagagctct tcaccaaatt
      841 ctgaatctgt aagtatttca accccgacaa taattatact cgctggaagg tccattccga
      901 gagatttagc aagttcaatg gcagtaataa aacttaaatc atggacattg gatagatgta
      961 ttgtctccct gaagtcagaa atatcaaaaa aataaacatc tccaggtttg ccttcccctg
     1021 tatggattgc atcaataaaa accacacagt catatcctga aatcagttct gctatttcaa
     1081 tacctccaca gcaggcttcc ttaaaactaa tattattgtc atcaaacacc aaagaaagat
     1141 cgcggataat ttttgggccg attccatcat ccgaaagtat tgtatttcca ataccaagaa
     1201 ccagagtttt tttattcttt accatttaca atacattaaa ctgaatttca attctaacta
     1261 ttccggctga tacatcatct tcattctcaa ttctgtccca tgcaatccag tttttttctt
     1321 tctaccgcca ctttaacgtc tgaaggtctc atttaataaa gatgttcatc catttaaata
     1381 gtgttatgca aataacggca aatttaattt attaaaagtt ccttttaaat ctgaattatt
     1441 catttattca tagcatgatt ttttggatat atatcaatga aagaaaataa aatcgcactt
     1501 aaattaaata ctggtttcag actgcagggg gacgacaggt ttgttaaaat gtaatataaa
     1561 aatttcttaa ataatgtttt gtagctatac aatctgtata taatatttca agtcagaatt
     1621 tcaaactcta tccctatttt ctttaaggca tatccggttt cttttaaata atcaccatat
     1681 acagtcaacc aatgaaaacc tcgcatattt tttgccacct tttcgctctc acacgaaaag
     1741 ctcacttcaa tctgtgaacg gcaaatgtcc ataaacggat tttcaacaat cttgccggga
     1801 agaccgatgt attttctgaa ttcaaaatca ggtatgacat ttgtcacaac ctggcctttt
     1861 ttcatttcga ccttaggagc ggctccgaaa tctgactcaa aatgggtcat tattcgtgca
     1921 ggctcaagcg ttattccatt cattctgcgc ggagcagtgc agtgtgcaag agtaatgagc
     1981 caatcatgtg gatatgtagg atcattcaga aatgatggtt tgcctgatat gttagccagc
     2041 aggatgccag caggaataac tacaaaatcc gattcacaga atgccaggaa tccggcatcg
     2101 ttgagaagac tgagtgtaag acaggctgtt gtatcagcaa tcggcataat tgttcccatg
     2161 caattattaa cagttattgc cctgcatcct tccctttcca taagcctgcg aaaaacttcc
     2221 ccgagcacga aacaattctc cacatacttt ctttcagttt caagcttaat cccggtatct
     2281 gccagatatc tgtcactact gctttttgcc tgcttaacga gtgccctgtc ttcccttgct
     2341 tctttaatca gattcccgag attttcataa gatatatccc ttatttcaaa gttgtaatgc
     2401 tcctttacca ttttcattgt tgtttcgagc ggctgcgacc atgctccgaa acctcctata
     2461 gctagaattc ttgaacctct tgtgtttttc agcccacata gtgccctcat ccgccacaag
     2521 agctcatcct ggctgtcaat aacaacatca ctgtcatcaa cccctttaac tttcaattca
     2581 tctgtatgct ggcgaaggta acgcggactt attatctcat accagagata aaccggacca
     2641 gacctgtggc ggcagaagaa aataatatct ttccccctgc tgtttaattc tgcaaaaata
     2701 ttcatgccgc caccagcagc atatataaga atcagatctg ccctgtctat gtcagttatt
     2761 ccggccagat cctgccggct ccgtacagca gagacaggaa gaaaatcaac cggaaaatca
     2821 gcatcagatt ttagcttttt aagctcgctg ttaattcttg ctatttcagc cgaggcgtca
     2881 ttttcggtct gtattccacc ccatgctctc cagcttgttt tctccctcct ttcaggcact
     2941 tcataggtga gaacaggttt gacaaccaga ggttgccttg ctggaagata ttcatctact
     3001 ggcctttcat tggccagcat atcccatgta agcctctgaa ttgttattat accaagagtg
     3061 ccggcacctg tcatctgcac aaactttcgc ctgctgatgc cctgatgccc catgtcagtg
     3121 tttccgcaac agcatcttaa agagctgtgc ttatgaaaat taactgaatt gtccaattta
     3181 tcatccatat ccttataaat aagatgctgt tatttttaca ttattagact cctgattctg
     3241 acaataattt aactaaaaat aataaaaata ttccttttga atatgaggta ttttttattt
     3301 cctgtctgat cccctctgtc atagcttaaa ttaacaaaaa caaagaaaat tctgtttgaa
     3361 tcattcataa aaaggttatc aataataatt gccataataa aatctcaccg ataatagtct
     3421 gaaattaaac aacctgcctt ctcgattgtt ataatattaa aattacgtcg aatattattt
     3481 aaaataatat ggctctgttt gaaataaaaa tgcctaaact gggagagagt ataacccagg
     3541 caactattat caggtggttc gtcaaagaag gtgactatgt taaagaggat gatgttctgc
     3601 ttgatatcgc aactgataaa gtagattctg aaattccgtc gcctgtagct ggaactgtaa
     3661 aaaagatatt ctaccaggaa aatgccattg ttcaggttgg ggaagttatt gcaattatag
     3721 atactgaagc aaactcttca ggaaaaaata aaaacataga tgaaaaaaat agcgggttgc
     3781 agcaaactga aaaaaaccag ctggaggaga aaaatttaaa tacctctgat gaaataatta
     3841 aaccagataa gaaagaagcc tcagcaagat tttactctcc gcttgcaaag ctcattgcca
     3901 gaagggaaaa tatttccgca gaagaactgg agaaaatacc gggaagcggg ataaatggga
     3961 gggttcagaa gaaagatatc cttgcttacc tggaaaaaag gaaagtcgct tcagcaactt
     4021 cggcatccgt tgttaatgac catactcctg ttaagagcga ggcattgaaa tattctatgc
     4081 ctgtctatca ggaagatgaa ataattgaaa tggaccgtat acggaagatt acggccgagc
     4141 acatgatcag gtcaaaacag acttcagctc atgttactaa tattcttgag gctgatgtaa
     4201 ctgcaattgt taactggaga aataaaatca aagatgaatt tgagaaaagg gaaggtataa
     4261 agcttactta tcttccttta ataatagagg cagtaataaa atccctcaag gaatttccga
     4321 tgctgaattc ttcggttgac ggaaacagga taattcttaa gaaaagaata aatataggga
     4381 tagcagttgc attgcctgat tcaaatctga ttgtccctgt aataaaaaat gctgattacc
     4441 tcaatataac aggacttgct aaagaaataa accgacttgc agaaaacgcc cgtaagaatg
     4501 accttcttcc ggatgatgtg aacgacggaa catttactgt tacaaatttc ggaactttcc
     4561 gcaatacaat aggtactcct ataataaatc agccccaggt tgccattctt ggaatcggaa
     4621 cggttgagaa aaagccgtct gttttagaaa ctcccgacgg agatgttatt gcgatacgcc
     4681 ataaaatgtt tctttccctt acatatgacc atagaattat taatggggca tatgcaggct
     4741 catatctgag aagactggcc gattatcttg aaacttttga tacagaaaga actatctgaa
     4801 ggtgttatta aattaaaatt tacaattatg tcagcaaaca aaaaatatca catcaagacg
     4861 accgataaat cagtacttga aaggtggtac agactgatga ctcttggcag agctcttgac
     4921 gagagggcac caaattatct gaagcagtct atcggctggt catatcatgc accctatgcg
     4981 ggacatgatg gtattcagct tgccataggg caggtttttg agaaaaataa agatttcctt
     5041 ttcccgtact acagggatat gcttactaac ctgtcggcag ggctgacagc aaccgagatt
     5101 attcttaacg ggatttcaaa ggcaaccgac cctgccagtg gcggacggca catgtcaaac
     5161 cactttgcaa aacctgaatg gaatattcag aatgtttcat catgtacagg caaccatgca
     5221 cttcatgctg ttggtgttgc acgcgcaata aggaaatatc gcgccgacgg tgtcgcaata
     5281 tgttcaatgg gagaatcggc tgtatcggaa ggctacgtct atgaagcgat aaatggggca
     5341 tcgcgtgaac agcttcccgt catcttcgta atacaggata acggatacgg aatttccgta
     5401 ccaaaaagcg accagactgc caaccgtaaa g
//
LOCUS       DAOSNB010000175         5431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15165_length_5431_cov_3.677269, whole genome shotgun sequence.
ACCESSION   DAOSNB010000175 DAOSNB010000000
VERSION     DAOSNB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_5431_cov_3.677269"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLG42_07605"
     CDS             complement(<1..376)
                     /locus_tag="PLG42_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31112.1"
                     /translation="MKGTLKYNLSFLAAIILIPSTLLHGQSLQQASPEVLLGEFCKTS
                     PWEELYLVTDRHDYIAGEDIWLKIFTIDRGTGLVVDRSKIAYVELLNPWNRPVIQLRF
                     GLNHGTGEGNIFLPDTLSPGNYT"
     gene            complement(391..1482)
                     /locus_tag="PLG42_07610"
     CDS             complement(391..1482)
                     /locus_tag="PLG42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOU31113.1"
                     /translation="MIFLLLLVPASGCIEEFIPDVEETKEMIVVEGLVTDQNRTNRVK
                     ISKSLPIGRALARKPVRAAKVTIIDDLGFVTTLKETDPGIYSTDSTSFRGVVGRKYAL
                     RIESSGFIYETDYIEMKPVPPIDSLYWEKILITQSDNPAEIEEGCKIYLDTHDPNRKC
                     FYFRWEYEETWEFWLPYQVPNNRCWVTEKSDRIMIKNASIYNQPKVTKFPVTFITNET
                     DRLKVRYSILVKQYSLNADEYKFWEKIQNVSENVGSLYDVIPMAIKGNIRCTNKPSET
                     VLGYFSVSAVTEKRIFIKDWFKGIPNFYWYCPTDTIFGRLPEEGRNVTWWVIEDGSSY
                     RPPYWVTTIHRECADCTTRGTNIKPSFWP"
     gene            complement(1512..4244)
                     /locus_tag="PLG42_07615"
     CDS             complement(1512..4244)
                     /locus_tag="PLG42_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU31114.1"
                     /translation="MRFRNLILIAFFLIFYNASAQQNYFVDWNYEGQSFYDFILKAES
                     KFPVKFFFREEWLKGITLSRYENKRYLNEILDTLLNGQGLFHYSDESGNIILTKYFAI
                     KPLIEEKAAGESYIPGLEYEEEDETKGAGGNIVIDLGNPADRDKPGIVTISGYITDQH
                     TREPVAGATVQIPKLSAGDISNSYGFYSLSVPRGSYSMKFSFIGMKEKTVDVNVYGSG
                     ELNVEMRSVLIPLKEALITAERNVTLQRFEVGLEKIDVNIFRLLPTAMGESDIMKSVL
                     LLPGVHSVGEGSAGFNVRGGSADQNLILLYGAPVYNSSHFFGFFSAVNPDIIRDVALF
                     KGGIPARYGGRLASVLDITSRDGNRREFAGNAGISPVTTHFVLEGPIKKDTIFYIIAG
                     RTTYSNWLLGMLQNRALRNSKASFHDINVRLAYDINRNNKIDLSSYYSYDSFQFNGDT
                     TYQYQNNIIALRWRHFFTSRLFTSLTLNNSFYGYNVSSTRVSQEAFKLSHKINSTGLK
                     ADFNWYTGRNEFNFGAEITEYNVRPGDYMPVGDSSLVIPNTVQSQRALEPSLWFEDKY
                     TLTDYLSVNAGIRLTSFLAAGPQTIYLYNKGYEKGPSSVKDTLFYGKNSIYKVYGGPE
                     IRLSVNIKTDTKSSVKLNYNHTKQYLHMLSNTTSISPTDIWKLSDYHLRPQTADQVAA
                     GYYRLLHKNRIEASAEIYYKKIRDMADFKGGTEIIMNEFIEWDLINVEGKAYGIELML
                     KKTEGRVRYNIGYTYSRVLVRSTSSISEEIINDGKWFPANFDRPHDLILTFNYLFSRR
                     YSFSATYNYSTGRPVTYPVATYTIGDIVIMQYSDRNKYRLPDYSRLDISLKVSGNLKA
                     KKIANPHWIFSVYNVLGRQNVYSVFFRNDKNMVKGYKLSVFGRPIPTVSFNFDF"
     gene            complement(4258..4971)
                     /locus_tag="PLG42_07620"
     CDS             complement(4258..4971)
                     /locus_tag="PLG42_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31115.1"
                     /translation="MRYSVATHITILSLLFTFISFLPVCGQEALIFNNIKPSNADSQL
                     LLNGRIWYNLYSRAFGHQFFATTEFLTGDVTLEGKQYKDLNLKYDILNDELLLSVPYK
                     PVLIMNREMVDSFSLNYNNRIYRIVNMDADSGSAIRGYVNVLYNGPTALYVKYMKEIQ
                     PLAVDGKYDRIIEKHKIFIKKDTAIVRISGRADLMKQLQDKKREIREFIRKNNYQVGR
                     IDPYTFIPVLEFYDSVKRK"
     gene            complement(4975..>5431)
                     /locus_tag="PLG42_07625"
     CDS             complement(4975..>5431)
                     /locus_tag="PLG42_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU31116.1"
                     /translation="NDPVQPEGQPRIRYGNIRTEQEFEDFNLKLEVNIPQGSNSGIYL
                     RGMYEVQVMDSYKRPLDSHNMGALYSRITPTVSAEKPAGEWQTLDITLCDRHLTVILN
                     GVKIIDNQPVYGPTGGAIKSDVFSPGPIYLQGDHGKVMYRNIVLTPIIK"
BASE COUNT         1433 a         1135 c         1100 g         1763 t
ORIGIN      
        1 gggtgtagtt gcccggactg agagtatcag ggagaaagat gttaccttct cccgtaccat
       61 gattgagccc gaaacgcaac tgtatcacgg gacggttcca ggggttaagc agttcaacat
      121 aagctatctt gcttctatcc acaaccagac cggtccccct gtcgatggta aatattttta
      181 accatatatc ctcaccggct atataatcat gccggtcggt aactaaatat aattcctccc
      241 acggtgaagt tttacagaat tccccaagga gaacttccgg gctggcttgc tgtaatgatt
      301 gaccatgcag aagagtggag ggaatcagaa tgattgcagc taagaatgac agattatatt
      361 ttaaagtccc tttcatttct tcattcagct ttatggccag aaagatggtt taatgttcgt
      421 tccacgcgta gtgcagtcgg cacattccct gtgtatggtt gtaacccagt atggtggcct
      481 gtaagagctg ccatcttcaa taacccacca tgtgacattc cttccttctt caggcagcct
      541 gccgaaaatg gtatcagttg gacagtacca gtaaaagtta gggatccctt taaaccagtc
      601 tttgataaaa atcctttttt cagtgactgc tgaaacgctg aaatatccaa gaacagtttc
      661 agagggtttg ttggtgcacc ttatattacc ttttattgcc attggaataa catcgtacag
      721 gcttcctacg ttttccgata cattctgaat tttctcccag aatttgtatt catcagcatt
      781 tagcgagtac tgcttgacaa ggatactgta tctaactttt aacctgtcgg tttcgtttgt
      841 tatgaaagtc actggaaatt ttgtaacctt cggctggttg tatatagacg cgttttttat
      901 cattatcctg tccgatttct cagttaccca gcatcggtta tttggaacct ggtaaggtaa
      961 ccagaattcc catgtttctt catattccca tctgaaatag aaacacttcc tgttgggatc
     1021 gtgtgtatcg agatatattt tgcatccttc ctcaatttca gcaggattat ctgattgtgt
     1081 tattaatatt ttttcccagt aaagggaatc aatagggggg acaggcttca tctcaatata
     1141 atctgtttcg tagataaatc ctgaagattc aattcggaga gcatacttcc ttcctacgac
     1201 accccgaaaa ctggtactgt cggtagaata aatgcccggg tctgtctctt ttagtgtagt
     1261 cacaaaaccc aggtcatcta ttatagtaac ttttgctgct ctgacaggtt ttctcgcaag
     1321 agcccttcct attggaagtg attttgatat tttaaccctg ttcgtccggt tctggtcagt
     1381 taccagtcct tccacaacaa tcatctcttt tgtctcttca acatcaggaa taaattcttc
     1441 tatacatccc gaagccggaa caagcaataa gagaaaaatc aatataaatt tcacccagtt
     1501 gttcatggta cctaaaaatc aaagttaaag gagacggttg gtataggtct cccgaatact
     1561 gacaacttgt agcctttcac catatttttg tcattcctga agaagactga gtaaacattc
     1621 tgtcttccaa gaacattgta caccgaaaag atccagtgtg gattggctat tttttttgcc
     1681 ttcaggttgc cgctcacttt aagcgaaata tcaagtctcg aataatctgg caaccggtac
     1741 ttgtttctgt cggaatactg cataattact atatcaccta tcgtatatgt tgctacagga
     1801 taggttacgg gtcttcctgt gctgtagtta tatgttgctg agaaactgta tcttcttgaa
     1861 aaaagataat taaaagtaag tatcaggtcg tgcggcctgt cgaaatttgc cgggaaccat
     1921 ttgccgtcgt ttatgatttc ctcacttatt gagcttgtgc ttcgtaccag tactcttgaa
     1981 tatgtatatc caatattata acgtactctg ccttcggtct ttttaagcat cagttctatt
     2041 ccataagcct taccttcaac atttatcaga tcccattcta tgaattcatt cataatgatt
     2101 tcggtacctc ctttgaaatc cgccatgtct ctgattttct tgtaatatat ttctgcagaa
     2161 gcttcgattc tgtttttatg cagtaatctg taatatccag ctgcaacctg gtctgctgtc
     2221 tgtggtctga gatgataatc actaagcttc catatatcag ttggagaaat ggatgttgtg
     2281 ttcgacagca tatgaaggta ttgttttgtg tgattgtagt tcagttttac agagctctta
     2341 gtatcggttt ttatgtttac tgacagtctg atctcgggcc ctccgtatac tttatatata
     2401 ctgtttttgc cataaaaaag tgtgtcttta actgatgatg gtcctttttc atatccctta
     2461 ttgtaaaggt atattgtctg gggacctgca gccagaaatg atgtaagtct tatgcccgca
     2521 tttaccgaaa ggtagtcagt taaagtatat ttatcttcaa accacaggga tggttcgaga
     2581 gctctctggc tctgtacggt gtttggtatt actagtgaag aatcaccaac gggcatataa
     2641 tctccagggc gcacattgta ttctgttatt tctgccccaa aattgaattc gtttcttcca
     2701 gtataccagt taaagtcagc cttaagacct gtgctgttaa ttttgtgcga tagtttaaac
     2761 gcttcctgtg aaacacgagt gcttgaaaca ttatacccgt aaaagctgtt attaagtgta
     2821 agtgatgtaa ataacctgct ggtgaaaaaa tgcctccagc ggagagcaat gatattattc
     2881 tgatactggt atgttgtatc gccgttgaac tggaaagaat cgtaactgta atacgatgag
     2941 aggtcgatct tgttgttacg gtttatatca taagcaagtc tgacattgat atcgtggaac
     3001 gatgctttgc tgtttcgcag tgcgcggttc tggagcatcc cgaggagcca gtttgaatat
     3061 gttgttcgtc cggcaatgat gtaaaagatg gtgtcttttt ttattggtcc ttcaagcaca
     3121 aaatgcgttg ttacgggact gatacctgcg tttccggcaa attctctcct gttgccatcc
     3181 cgggaagtga tgtcaagaac tgatgcaagt cttccaccgt agcgtgcagg gataccgcct
     3241 ttgaagaggg ctacatctct tattatatcg ggattaacag ccgagaaaaa accgaagaaa
     3301 tgtgatgaat tgtaaaccgg ggcaccgtag agcaatataa gattttgatc ggcagagcca
     3361 ccgcgtacgt taaatccagc cgaaccttca cccactgagt gcactcccgg caggagaaga
     3421 acactcttca tgatgtccga ttcgcccatg gctgtcggga gaagcctgaa gatatttacg
     3481 tcaatctttt caagaccaac ctcaaaacgc tgcaaggtca catttctttc ggcagttatc
     3541 aaagcttctt tcagaggaat gagtacactt ctcatttcaa cgttaagctc acctgaacca
     3601 tatacgttga catccacagt cttttctttc attcctatga atgagaactt catagaatag
     3661 ctgccccggg gcacactgag cgagtagaag ccataagaat ttgaaatgtc gccggcagat
     3721 agtttaggaa tctgtacagt ggcaccggca acgggttccc ttgtatgctg gtcagtaata
     3781 taccccgaaa ttgttacaat gccgggtttg tctctgtcgg caggattacc taagtcaatt
     3841 actatgtttc ctccagcacc tttagtctca tcctcttctt catattccaa tcctggtata
     3901 tatgactcac ctgcagcttt ctcttctatc agaggtttta tcgcaaaata tttcgtcagg
     3961 ataatattgc ccgattcgtc ggaatagtga aataatccct gaccgttgag aagtgtatca
     4021 aggatttcgt taaggtatct tttgttctcg taccggctca gcgttatacc tttgagccat
     4081 tcctccctga aaaagaactt aaccgggaat tttgattcag ccttaaggat gaaatcgtaa
     4141 aatgattgcc cctcataatt ccaatccaca aaataattct gctgtgcaga tgcgttgtaa
     4201 aaaatcagaa agaaggcaat caatattaag tttctgaatc tcatccctgt aatgttctca
     4261 ttttcttttg actgaatcgt aaaattcgag aacgggaata aaagtataag gatcaattct
     4321 cccgacctga taattgtttt ttcttataaa ttctctgatt tcacgttttt tatcctgaag
     4381 ctgtttcata agatctgctc tccccgaaat acgaactatc gcagtatcct tcttaataaa
     4441 aattttgtgt ttctcaatga ttctgtcata tttaccatct actgcaagtg gctggatttc
     4501 cttcatgtac ttaacataaa gagctgttgg tccgttatat agtacgttga catatcccct
     4561 tattgcgctg cctgaatcgg catccatgtt tacaattctg tagattctgt tattgtaatt
     4621 gaggctaaag ctatcgacca tttccctgtt cattattaaa acaggtttgt aagggacaga
     4681 cagcagaagt tcatcgttta atatgtcgta tttcaggttg agatctttgt attgttttcc
     4741 ttcaagggta acgtcccctg tgaggaattc ggttgttgcg aaaaattgat ggccgaatgc
     4801 cctgctataa agattatacc atatccggcc gttgagcagc agctgggaat cggcatttga
     4861 aggtttaata ttgttaaata ttaaagcttc ctgcccgcaa acaggcagaa agctgatgaa
     4921 agtaaacagt aatgaaagta ttgtaatatg ggttgcaaca gaatatctca cgtcctattt
     4981 tattatagga gtgagtacaa tgttccggta cattactttc ccgtggtcgc cctgcaggta
     5041 tatcggtccg ggtgagaaca catccgattt tattgccccg cctgtgggtc cgtaaacagg
     5101 ctgattatca ataattttta ctccgtttag aattactgtt aaatgtctgt cgcacagtgt
     5161 tatatcgagt gtctgccatt caccggcagg tttttctgca ctgacggtgg gtgttattct
     5221 gctgtaaagt gctcccatat tatgtgagtc gagaggcctt ttgtatgaat ccatgacctg
     5281 gacttcatac attccacgca ggtatatacc gctattgctt ccctgaggta tattgacttc
     5341 cagtttcagg ttgaaatctt cgaattcctg ttcggtccgt atgtttccgt atctgattct
     5401 tggctggcct tctggctgaa cagggtcatt a
//
LOCUS       DAOSNB010000176         5410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15239_length_5410_cov_5.328105, whole genome shotgun sequence.
ACCESSION   DAOSNB010000176 DAOSNB010000000
VERSION     DAOSNB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15239_length_5410_cov_5.328105"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /gene="ftsH"
                     /locus_tag="PLG42_07630"
     CDS             complement(<1..2004)
                     /gene="ftsH"
                     /locus_tag="PLG42_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOU31117.1"
                     /translation="MPEERRENSNQGKQGDKNQKPRFNSNWIFAIIALSILFFQIFYG
                     GKTVEKINTGKLRELLTSREIEKIIVVNKDQAEIYLNEKALESDKHSKVAKNTSATGL
                     SGPRPHYIYNIGDLSRFETFLIDTQKASGYTDDEIIYPEYINRKNYLADILGWMLPFL
                     FLLVLWIFIMKKVTGGAGGGSGGIFSVGKSRPQIFDKDTSVKINFSDVAGLEEAKTEV
                     MEIVDFLKNPKKYTQLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGSEFV
                     EMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAIGRARGRNPNFGANDERESTLNQLL
                     TEMDGFGTNMGVIILAATNRADILDRALLRAGRFDRQIHVELPDLKEREAIFKVHMRP
                     LKLEKDLDVSFLARQTPGFSGADIANVCNEAALIAARKNKTVIEKQDFLDAIDRIIGG
                     LEKKNKIISYEEKKTIAYHEAGHATVSWLLEHASPLVKVTIIPRGKALGAAWYLPEER
                     QLTTREQILDEMAYAMGGRAAEQLVFGKISTGALSDLEKVTKQAYAMVAYFGMSEKIG
                     NISFYDSTGQADFNFTKPYSEKTAELIDKEVKKIVDEAYQRALNVLEKNKKGLVKLAE
                     LLLEKEVIYSEDLEKIFGKRKAEVVKPAGETVSGEKEKKDSEIT"
     gene            complement(1979..2389)
                     /gene="rsfS"
                     /locus_tag="PLG42_07635"
     CDS             complement(1979..2389)
                     /gene="rsfS"
                     /locus_tag="PLG42_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOU31118.1"
                     /translation="MKKKPDGTELLIKSVVKGIFEKQGKDVIKIDLRTFENRITDYFI
                     ICHGNSVTQVDAITSSVEEMVRKETGEKPSHIEGLENCFWVLLDYIDVVVHIFQEEYR
                     NFYSLESLWADAEIEKLEDIVKVKKNYAGRKKRK"
     gene            2533..3270
                     /locus_tag="PLG42_07640"
     CDS             2533..3270
                     /locus_tag="PLG42_07640"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOU31119.1"
                     /translation="MIIGSRKVYFEKLSSTNTHAAAMLRKGRVLEGTIVYTGFQTAGK
                     GQAGNRWESEKDKNLLLSVILYPTTIRADRQFLISKVISLGVSDFLRTYVYDVTIKWP
                     NDIYVKNEKIAGILIENSIIRNEIDHSIAGIGININQQHFPRHLPNPTSLKILTGKDY
                     DLEQCLNELAAKLDARYKLLLYNRIGRIEEDYKNSLYRFGQFTEFSDNRGVFEGKIIS
                     VTDTGRIQIEDRRGRIYEYGFNEVDFL"
     gene            complement(3267..3683)
                     /locus_tag="PLG42_07645"
     CDS             complement(3267..3683)
                     /locus_tag="PLG42_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31120.1"
                     /translation="MIYSNERTVKVSCGKQDFYNFVTDIRNFGRFIPESLRQGWNASE
                     EKCSFSLSGMGEMKIGISEKIPEEKVVFSGTALSKISFIIETIITGGAEVNSCDVQVT
                     LRAEMDRLTGIIAKPQLDAVFGAIVNEMENFSGWND"
     gene            complement(3688..4320)
                     /gene="pyrE"
                     /locus_tag="PLG42_07650"
     CDS             complement(3688..4320)
                     /gene="pyrE"
                     /locus_tag="PLG42_07650"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOU31121.1"
                     /translation="MENVSKKIAEYLLQIKAIKLQPSNPFTWASGWKSPIYCDNRKTL
                     SFPEVRTFICDSFIQAVKSYYPDVEVIAGVATGAIAHGALVAQKMGLPFIYVRSEAKS
                     HGLGNQIEGYFEKGQKVVVIEDLVSTGGTSLGAVRALREAGCEVLGMIAIFTYGFQKA
                     AEGFLSENCELHTLSDYNTLIDCALETGYIGQADVETLREWRKDPSKWGV"
     gene            4321..4938
                     /locus_tag="PLG42_07655"
     CDS             4321..4938
                     /locus_tag="PLG42_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU31122.1"
                     /translation="MTTYKVHFENRYILLSREPDRLQKYSLFHKYSTTSELYKLIATF
                     QEDKSIHSVNIYGPDITHIWKLFRIYFSEVQAAGGLVKHTSGRYLFIRKRGKWDLPKG
                     HVDKNETPEECALREVQEECGITGCTIIKPLEPSYHTYTIEGISYLKKTSWFLMDYSG
                     EMIKKPEEREHITEVAWLLPEEIPDIKKDLWLSLTDLIYKHVLTD"
     gene            complement(4928..>5410)
                     /locus_tag="PLG42_07660"
     CDS             complement(4928..>5410)
                     /locus_tag="PLG42_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31123.1"
                     /translation="CNLALILKQNATALAPGTFPPPFYLPDSSGRMVTAGDFQGKFLL
                     LVFIKETDYASLSELALLKSWISNFKDNVILAAVITGKNYRDMLSYFRKRGYDWLFLD
                     GTVNPFLADLYDLRIYPSFMLLDREGRIAVRYCPSPSENLEGFLKSILEKESRKIISR
                     "
BASE COUNT         1533 a         1272 c          991 g         1614 t
ORIGIN      
        1 agtaatttct gaatctttct tttccttttc ccctgaaacg gtttcgccgg caggctttac
       61 cacttcagct tttcttttac cgaatatttt ctcaaggtcc tcgctgtaga ttacttcttt
      121 ttccaggagc agttcggcga gtttaacaag tcccttctta ttcttttcca gaacattgag
      181 tgctcgttga taggcttcat ccacaatctt ctttacttcc ttgtctatca gttctgccgt
      241 tttttcgctg taaggtttgg tgaaattgaa gtcggcctgt cctgtagagt cgtagaagct
      301 tatattgcct attttttcac tcataccaaa gtaagccacc attgcataag cctgtttggt
      361 aactttttcg aggtcgctga gtgctccagt tgatattttc ccgaacacca gttgttctgc
      421 tgctcttccg cccatagcat atgccatctc gtcgagtatc tgttcgcgtg ttgtcagctg
      481 gcgctcttcg ggcagatacc atgctgctcc gagtgctttc ccacgtggaa ttatggtcac
      541 ctttacaagc gggcttgcat gctcaagcaa ccagctcaca gttgcatgtc cggcttcgtg
      601 gtatgcaatg gtcttttttt cttcgtatga tattatcttg tttttcttct caaggccacc
      661 tataattctg tcaattgcat caagaaagtc ctgtttttct attacagttt tgttcttcct
      721 tgcagctatc agggctgctt cgttgcatac gtttgcaata tcggctcctg aaaaacctgg
      781 tgtctggcgg gcaaggaagg atacatcaag atctttttca agctttaatg gacgcatgtg
      841 taccttaaag attgcttccc tttccttgag gtcgggaagc tcaacatgta tctgcctgtc
      901 gaacctgccg gcacgcagaa gtgccctgtc gagaatatcg gcacggttgg ttgctgccag
      961 aataatcact cccatatttg ttccgaagcc atccatctct gtaagcaact gatttaatgt
     1021 gctctccctt tcatcatttg cgccgaaatt aggattacgc cctcttgccc ttcctatggc
     1081 atctatctcg tcgatgaaga caatacatgg tgccttctct tttgcctgtt tgaacagatc
     1141 ccttacacgt gaggcaccta ccccaacaaa catttcaaca aactccgaac ccgagattga
     1201 aaagaaaggc acattggctt cacccgctac agcctttgca agcagtgtct tacccgttcc
     1261 cggaggacca atgagaagtg cacccttggg tatcttaccc cccagctggg tgtacttctt
     1321 cggattcttc agaaaatcaa ctatctccat cacctcggtc tttgcctcct caagacctgc
     1381 aacatcgctg aaattaatct ttacactcgt gtctttgtca aatatctgcg gcctcgattt
     1441 tccaacactg aaaattcctc ccgaacctcc tccagcacct cctgtaacct tcttcataat
     1501 aaaaatccag agaacaagca ggaacaaaaa aggaagcatc caaccaagta tgtcggccag
     1561 ataattttta cggttaatat attcgggata aattatctca tcatcggtat aacccgacgc
     1621 tttttgagta tcaatcagaa atgtttcaaa ccggctcagg tcgccaatat tgtaaatata
     1681 atgaggtctt gggccagata aacctgtagc ggaggtgttt ttggcaactt ttgagtgttt
     1741 atccgactcc agcgcctttt cattcaggta aatctctgcc tggtctttat tgacaactat
     1801 aatcttctca atttcacgac tggtaagtaa ttccctgagt ttaccggtat tgattttctc
     1861 aacggtttta cctccgtaaa atatctgaaa aaacaaaatg gataatgcaa tgatggcaaa
     1921 aatccagttc gaattgaacc tgggtttctg gtttttgtca ccctgctttc cctggttact
     1981 attttctctt ctttcttccg gcataattct tttttacctt aacaatatct tcaagttttt
     2041 caatttctgc gtcagcccat aacgattcca ggctgtaaaa atttctgtac tcttcctgaa
     2101 aaatatgtac cacaacatcg atataatcaa gaagcaccca gaaacaattc tccagtcctt
     2161 ctatatgaga cggcttttca cctgtctctt ttctaaccat ttcctcaaca gaactcgtaa
     2221 tagcatctac ctgagtaaca gaattcccgt ggcaaattat aaagtaatcc gtgatcctgt
     2281 tttcaaaagt tcttaagtct attttaatta catccttacc ctgcttctca aaaataccct
     2341 tcacaacact ttttataagc aattctgttc cgtcaggctt ttttttcatt aaaaaattct
     2401 ctttattttg caaatgcaaa agtacaaaca attatcagta tttttgtaaa agcctgccaa
     2461 tttttgcaag attaataccg tattgtatta aattaactgt aaaaacatta acttttacaa
     2521 acaaatatta ttatgataat cggttccaga aaagtctact ttgaaaaatt atcatcaacc
     2581 aatactcatg cagcagccat gctgagaaaa ggcagggtac tggaaggaac tatagtatat
     2641 acaggtttcc agactgcagg taaagggcag gccggaaaca gatgggaaag tgaaaaggac
     2701 aagaatctgc tgctgagtgt tattctctac cccaccacaa tcagggccga cagacagttt
     2761 ctgatttcaa aagttatttc actgggtgtg agtgattttc tgaggacata tgtgtatgac
     2821 gttacaataa aatggcctaa cgatatctat gtaaagaatg aaaaaattgc tggaattctt
     2881 attgaaaatt ccattatcag aaatgaaatt gaccattcca ttgcaggcat agggattaac
     2941 atcaatcagc agcattttcc ccgccatctg cccaacccaa cttcactgaa aatcctaacc
     3001 ggaaaggatt acgaccttga gcagtgcctc aacgaactgg cagccaagct cgatgcccgc
     3061 tacaaactgc ttctttataa tcgcatagga agaatagaag aagattacaa aaattcgctc
     3121 taccgcttcg gccagttcac agaattcagt gataacaggg gtgttttcga aggaaagatc
     3181 atttcggtaa ccgatactgg tagaatccag atagaggaca gaagaggaag aatttatgaa
     3241 tacggtttca atgaggtaga ctttctttaa tcattccatc ccgaaaaatt ttccatctca
     3301 tttactatcg caccgaatac agcatcgagc tgtggcttcg caataatgcc tgtaagccta
     3361 tccatttctg ctcttaacgt aacctgcaca tcacaggaat ttacttctgc accaccggtt
     3421 attatggttt cgataatgaa gcttatcttc gaaagagccg taccactgaa aacaaccttc
     3481 tcctcaggaa ttttttcact gataccgatt ttcatttcac ccatgcctga aagagaaaat
     3541 gagcattttt cttccgaagc attccatccc tgccggagag actccggaat aaacctgcca
     3601 aaatttctta tatcggtcac gaagttgtaa aagtcctgtt tcccacaaga tacttttact
     3661 gttctttcgt ttgaatatat cattgcctta cacgccccat tttgatggat cttttctcca
     3721 ctccctgagg gtttccacat cagcctgtcc gatatatccc gtttcgaggg cacagtcgat
     3781 aagtgtatta taatcgctta aggtatgaag ttcacaattc tccgaaagaa acccctccgc
     3841 agctttctga aatccgtaag taaatattgc tatcatacca agcacctcac atcctgcttc
     3901 cctcagcgca cggacagctc cgaggcttgt cccgccggtg gacaccagat cttctattac
     3961 aaccactttc tgaccctttt caaaataacc ctctatctga ttgcccagcc catggctttt
     4021 ggcttcagac ctcacataaa taaaaggaag ccccatcttc tgagcaacaa gcgccccatg
     4081 ggcaattgcc cctgtggcta ctcctgcaat aacctcaaca tcaggataat agcttttaac
     4141 tgcctgaata aacgagtcac aaatgaaggt tcggacctcg ggaaacgaca gggttttcct
     4201 gttatcgcaa tatataggtg atttccaccc cgaagcccat gtaaaaggat ttgatggttg
     4261 taattttatt gctttaattt gcaataaata ttccgcgatt ttttttgaaa cgttttccat
     4321 atgacgactt ataaggttca ttttgaaaac aggtacattt tactgagccg ggaacccgac
     4381 cggttacaaa agtacagctt attccataaa tacagcacaa cttcggaact ttataagctc
     4441 atagccacat ttcaggaaga caagagcatc cactcagtaa atatttacgg acccgacata
     4501 acccatatct ggaaactttt ccggatttat ttttctgaag tgcaagctgc aggaggactt
     4561 gttaagcata cttctggaag atacctattc atcagaaagc gaggtaaatg ggatttaccc
     4621 aaaggacatg tagataaaaa cgaaaccccc gaagagtgtg cactgcgtga agtgcaagag
     4681 gaatgcggta ttacaggatg taccatcata aagccgctcg agccaagtta tcacacctat
     4741 accattgaag gtatttctta cctgaaaaaa acgagctggt ttttaatgga ttacagcggg
     4801 gagatgataa agaaaccgga agaaagagaa catatcacag aggttgcctg gctattgcct
     4861 gaagagattc cggatataaa aaaagatctc tggctctcac ttacagacct gatttacaaa
     4921 cacgtactta ccgactaatt attttccggc tctccttttc aagaatactt ttcaaaaatc
     4981 cttccagatt ctctgaaggt gaagggcaat atcttactgc aatcctgcct tctctgtcga
     5041 gaagcataaa ggacggataa attctcagat cgtataaatc ggccagaaac ggattaacgg
     5101 ttccatccag aaagagccag tcataacccc tttttctgaa gtatgataac atgtctctgt
     5161 aatttttacc cgtaatgaca gcagcaagga taacattgtc cttaaaatta ctgatccacg
     5221 actttaacag ggcaagttca ctcagtgaag catagtcggt ctctttgata aagacaagca
     5281 gtaaaaactt tccctgaaaa tccccggcag ttaccatcct gcctgaacta tcgggcaagt
     5341 aaaaaggtgg cggaaaggtg ccaggagcaa gtgctgttgc attttgcttc agaataagag
     5401 ccaggttaca
//
LOCUS       DAOSNB010000177         5375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15355_length_5375_cov_4.089474, whole genome shotgun sequence.
ACCESSION   DAOSNB010000177 DAOSNB010000000
VERSION     DAOSNB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15355_length_5375_cov_4.089474"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLG42_07665"
     CDS             complement(<1..1120)
                     /locus_tag="PLG42_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31124.1"
                     /translation="MHASGQEPKEMQDTLRKDALNVYMEASDFIKKEIPFINYVRDLK
                     DADVYIISTNQRTGAGGTEYTYFFTGQKRFTGINDTVTFASSPDDTQDVIRMKQVNAL
                     KMGLIRYVGKTPLAKYINVSFSMPLTESVSTDKWNNWVFRASVNGYLTGEKSYQSKYI
                     RGSLSASRVTNDWKINLSGSYYYSVSTYDISGYKYKSENNSKNLSGMLVKSLSDHWSA
                     GGFISTGSSSYSNEKFNAGIMPAIEYDIFPYSQSTRRQLRLLYRIGFNPVIYYDTTIY
                     DKTREYLWSHSFAAAYEVVQKWGSIDMNLNYSNYLHDWSKNNLSFSSYLNIRITKGLS
                     FNIGGGASLIHDQLGLVKGGATTEEILLRRRELETQYEF"
     assembly_gap    1230..1329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1434..2945)
                     /locus_tag="PLG42_07670"
     CDS             complement(1434..2945)
                     /locus_tag="PLG42_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU31125.1"
                     /translation="MDKTIKINLAGVLFIIDEKAYVALRDYLQSIDMRLKNVPGGNEI
                     IDDIEARIAEIFQSGKSESDIINTDDVNNVINIIGRPEEFEQEGDTERKNAYRIYRRR
                     LYRDINNPVLGGVCSGIGAYLNIDPVWIRLIFIIFTFAYAAGILIYVILWIAFPVAKT
                     EIQKRELYGENYYSKQTGSGSPETAKITGALNSFFLALGRIFRIVAWFFLAIAGAVMV
                     VTGFAMFVAFSLVFYLRYPVSFFHEGAGTGIFSISDFLELATSPAITPWIMVLISFVV
                     ILPLLALIYWGLKMIFRFKANELWLSISALVVWVISFTALLMILVSQGIDFAEEGRTS
                     EQIFPQHTPDTIYIKARNNIAEMKFDKVLTIPGVNYSLFLVKDGSILFSKPEIELYNS
                     EEGKFFISVEKTMFGSNKREAVKKAEALIFNYSFSGDTLYVDQYYEIPPGFKWSGSFI
                     ETGVYLPENTVIWIDKDTEILFGDEYCEVPDLGGKYWQWDGEKLISLLRGGTN"
     gene            complement(2962..3294)
                     /locus_tag="PLG42_07675"
     CDS             complement(2962..3294)
                     /locus_tag="PLG42_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOU31126.1"
                     /translation="MDIENTKAQMRKGILEYCILLVLSGSSCYASDIIRELKQVKVIV
                     VEGTLYPLLTRLKNAGLLSYRWEESQQGPPRKYYELTDEGKKYLKELDTSWMELVESV
                     NLIRKNMK"
     gene            complement(3316..3918)
                     /locus_tag="PLG42_07680"
     CDS             complement(3316..3918)
                     /locus_tag="PLG42_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOU31127.1"
                     /translation="MKITLSVNLGGYSFNIDEDAYEELSRYLKSLESSFASEKSASEI
                     MSDIEDRIAELFREKISGYKQVITIEDVREVISILGRPEDFAFEGEGTGSSRDDSSRY
                     SGRSHRMYRDPDNRILGGVCAGIGAYWRIEPWIIRIIFLALMFAGGFGLLIYIILWIV
                     LPEARTIAQKIEMRGEPVNIQSIKEAVKREFEQVRRRMNI"
     gene            4128..5126
                     /locus_tag="PLG42_07685"
     CDS             4128..5126
                     /locus_tag="PLG42_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31128.1"
                     /translation="MGKKYNWGILAPGKMSAKFTNALKILSNAELYAVGSRAVERARK
                     FAEEHGFRKYYGSYEELIADPEVEIIYIASPHSFHHEHTLMCLRNGKHVICEKAFALN
                     SHQVDEMISEARKRNLFLMEALWPPFQPFYKKAQEILLSGILGDIIHLHGWFSFQPPY
                     NPDDRKFNPALGAGSLLDIGIYPVIDALTFIGVPDEIKAFALFAPTGSEESLTAIFRY
                     NKGKMADIYSSFRTNEGIGCKLLCENGNMIVSRGRDMNQRVVLELHGKAPEEYIFRPE
                     ALGYHWEAEEVMRCLDNGLTESPVVPHSFSIDLIRTLDRIRKEAGVVFPGEQLTNS"
     gene            5134..>5375
                     /locus_tag="PLG42_07690"
     CDS             5134..>5375
                     /locus_tag="PLG42_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31129.1"
                     /translation="MTPPDNAKTYDPDLFYRKKFLLSIIIPATFVFLMWFVKITEILF
                     EADFSFLGIYPLSLKGLPGILFSPFIHGDFKHLYSNT"
BASE COUNT         1576 a         1110 c          871 g         1718 t
ORIGIN      
        1 aaaactcata ttgcgtttca agctcccttc gccttaaaag gatctcttcg gtggtagccc
       61 ctcctttaac aagtcccagc tggtcgtgaa taagcgatgc acctcctcca atattaaagg
      121 aaagaccctt cgttatcctg atattgagat aactggaaaa agaaagattg ttttttgacc
      181 agtcgtgaag gtaattgctg taattcaaat tcatatcaat agatccccat ttctggacaa
      241 cctcatatgc cgcagcaaat gaatgactcc agagatattc tctggtttta tcgtatatgg
      301 ttgtatcata atatattacc ggattaaatc caatccggta taacagtctt agttgtctcc
      361 ttgttgattg agaatacggg aaaatgtcat attcaattgc aggcataata cctgcattaa
      421 atttctcgtt tgaatagctt gatgatcctg tacttataaa tcctcctgcc gaccagtggt
      481 ctgaaaggct tttaaccagc atcccgctta aattcttcga attgttctca cttttgtatt
      541 tatatcctga aatgtcgtaa gtacttacac tatagtaata actgccactg aggttgattt
      601 tccagtcgtt tgtaaccctg ctggctgaaa ggcttcccct gatatatttt gactggtaag
      661 atttttctcc tgtaagatat ccgttcaccg atgccctgaa aacccagttg ttccatttat
      721 cggtcgaaac agactctgtc aaaggcattg aaaaagatac attaatatat tttgctagcg
      781 gagttttacc gacatacctt attagaccca tctttaaagc atttacctgc ttcatcctta
      841 tcacatcctg cgtgtcatcg ggtgatgagg caaatgtaac agtatcgtta attcctgtaa
      901 accttttctg gccggtgaaa aagtaggtgt attccgttcc tcctgcaccg gttcgttgat
      961 tggtagaaat tatgtagaca tctgcatctt taagatctct gacataattt atgaaaggta
     1021 tctctttttt gatgaagtcg gatgcttcca tgtaaacatt aagagcatct tttcttaaag
     1081 tatcctgcat ttctttaggt tcctgccctg atgcatgcaa aaccagcaag aaaaacaata
     1141 aaatagtaaa agccctgttt gataacattg aattatagta aaatattaat aatggctaat
     1201 ttacgcataa tttttattat acaactaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnna tagtaaaata ttaataatgg ctaatttacg cataattttt attatacaac
     1381 taaagattta aataaataat cgcatatttc ctctttcaca aaaaattcta ctgctagtta
     1441 gttcctcctc ttaagagtga aatcagtttt tctccatccc attgccagta ctttcctcca
     1501 aggtccggca cctcacaata ttcatcacca aatagaatct ctgtgtcttt atcaatccat
     1561 attacagtgt tttcaggaag atagactcca gtttcaataa acgagcccga ccatttaaac
     1621 cctggaggta tttcataata ctgatcaaca tacagggtat cacccgaaaa gctataattg
     1681 aaaatcaagg cttcagcttt ctttactgct tcacgtttgt tgcttccaaa cattgttttt
     1741 tcaacagata tgaaaaactt accttcttcc gaattgtaca actcaatttc cggtttagaa
     1801 aatagtatac tgccatcttt aaccagaaaa agggaatagt ttactcccgg aattgtaagg
     1861 actttgtcaa atttcatttc agcaatatta ttcctggctt taatataaat tgtatcgggc
     1921 gtatgttgtg gaaatatctg ctccgatgtt cttccttctt cggcaaagtc aattccctgt
     1981 gacactaaaa tcatcaacaa agctgtaaaa cttattaccc atactaccag tgctgatatg
     2041 cttaaccaca attcatttgc cttgaacctg aaaatcattt tcaaacccca gtaaattaac
     2101 gctagcagag gaagaatcac aacaaatgaa atcaaaacca ttatccatgg agtgattgca
     2161 ggactggttg ctaattccag aaaatcagaa atactgaata taccagttcc tgctccttca
     2221 tggaaaaacg aaacaggata tctcagataa aacaccagac tgaaagccac aaacatggca
     2281 aagccggtaa caaccataac agcccctgca attgcaagaa aaaaccaggc aactatcctg
     2341 aaaattcttc ccagtgcaag aaagaatgaa ttaagtgccc ccgttatttt tgcagtttca
     2401 ggtgaaccgc ttccagtttg ttttgagtaa taattttcgc cgtaaagttc tctcttttga
     2461 atttcagttt ttgcgactgg aaaggctatc cataaaataa catatatcag tatacctgca
     2521 gcataagcaa atgtaaaaat tatgaaaata agtctaatcc ataccggatc aatattcaga
     2581 tatgcgccta ttccgctaca gacacctcct aaaaccgggt tgttaatatc cctgtacagc
     2641 cttctgcggt agattctgta agcattcttt ctttcagtat ctccctcctg ttcaaattct
     2701 tctggccttc ctatgatgtt tatgacattg ttgacatcat ctgtgtttat aatatctgat
     2761 tcacttttac ccgactgaaa tatttcagca attctggctt caatatcatc aatgatttca
     2821 ttgccgccgg gaacattttt caatctcatg tctattgact gcaggtagtc gcgaagcgcc
     2881 acataagcct tttcatctat aataaaaagc acccctgcaa gatttatttt tattgttttg
     2941 tccattttgt acttgtttaa attatttcat attttttctt ataagattca ctgattctac
     3001 aagttccatc catgaggtat caagttcttt caggtatttt ttaccttcat cagtaagttc
     3061 gtagtatttc cgcggaggac cctgctgtga ttcttcccat cgataactta acaatccggc
     3121 atttttcagt cgtgtaagca acggataaag tgtcccttca acaactataa ccttaacctg
     3181 tttgagttcc ctgataatat cgcttgcgta acatgaactt ccagaaagta caagaagaat
     3241 gcagtattca agtattcctt tacgcatttg agctttagta ttctcaatat ccataatgtt
     3301 atattttaag tattttcaaa tattcattct gcgacgcacc tgttcaaatt ccctttttac
     3361 agcttccttt atgctttgta tgtttacagg ctctcctctc atttctattt tctgggcaat
     3421 tgttctggct tcgggtagta caatccagag aataatgtag attaacaatc cgaaccctcc
     3481 tgcaaacatc agagcaagaa atattattct tattatccag ggttcaattc tccagtatgc
     3541 accaattcct gcgcaaacac ctcccagaat tcggttgtca ggatcacggt acattcgatg
     3601 cgatcgtcct gaataccgcg acgagtcatc tctgctgctt cctgttcctt caccctcaaa
     3661 ggcaaaatcc tccggccttc ccagaataga aataacttcc cttacatctt caatagttat
     3721 tacctgcttg taacccgata tcttttccct gaaaagctca gctatcctgt cctcaatatc
     3781 actcattatt tcagaagcgc ttttttcaga agcaaaacta ctttcaagac tcttgagata
     3841 acggctcagt tcttcatagg catcttcatc aatattgaat gaatagcctc ccagattaac
     3901 ggacaatgta attttcattt tcttttattt aatatttatt ttttatatta cctgtcattg
     3961 caaagatata taaaaaatat actattatgc tatacctagt actaaaattt taaaaaattt
     4021 attttttcac tgctaataaa ttatttacgt actatctctc cgttgtgttt gctaaggcaa
     4081 acgtactcag ttaatttttt tatatttatc ggctaaaatc aatcactatg gggaaaaaat
     4141 ataattgggg tatccttgct cccggaaaga tgtcggcaaa attcaccaat gcattaaaaa
     4201 ttttaagtaa tgccgaactt tacgctgtgg gttcccgagc cgttgaacgt gcaagaaaat
     4261 ttgctgaaga acacggattc aggaaatatt acggaagtta tgaagaactt attgcagacc
     4321 ctgaagtcga gataatctat attgcatctc cacattcatt tcatcatgag cataccctta
     4381 tgtgccttcg caacgggaaa catgtaatat gtgaaaaggc tttcgcactt aactcccatc
     4441 aggtagatga aatgatttca gaagccagga agcggaatct atttctgatg gaagccctct
     4501 ggccaccctt ccagccattt tataaaaaag ctcaagagat attgctgtca ggaattctgg
     4561 gtgatatcat tcatctacac ggctggtttt cttttcagcc tccctataac ccggatgaca
     4621 gaaagttcaa tccggcactc ggagcaggtt cgcttcttga tataggtata taccctgtta
     4681 ttgatgcgct gaccttcata ggtgttcctg atgaaataaa agctttcgcc ctcttcgcac
     4741 ctactggttc tgaagaatca ctcactgcaa ttttccgtta caacaaaggg aaaatggcag
     4801 atatttacag ttctttcaga accaatgaag gtatcggatg caaactgctg tgcgaaaatg
     4861 gtaacatgat tgtttcaagg ggaagagata tgaaccagag agtggttctt gaactgcatg
     4921 gtaaagctcc tgaagaatat attttcagac cagaggcact gggttatcac tgggaagccg
     4981 aagaagttat gcgctgtctt gataatggct tgacagagag tccggtcgtt cctcattcat
     5041 tcagcattga tcttatcaga acccttgacc gtataagaaa agaagcagga gttgtctttc
     5101 cgggagaaca actcacaaat tcataaacat gggatgacac cacccgataa tgcaaaaact
     5161 tatgatcctg atttattcta ccggaagaaa tttcttctca gtataattat tccggcaact
     5221 tttgttttcc ttatgtggtt tgtgaaaata actgaaattc tgtttgaagc agatttttct
     5281 tttcttggaa tttatcctct gagcttaaaa ggtctgccgg gaatattgtt ttctcctttc
     5341 attcatggtg attttaaaca tctgtacagt aatac
//
LOCUS       DAOSNB010000178         5356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15418_length_5356_cov_4.169591, whole genome shotgun sequence.
ACCESSION   DAOSNB010000178 DAOSNB010000000
VERSION     DAOSNB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15418_length_5356_cov_4.169591"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PLG42_07695"
     CDS             <1..1121
                     /locus_tag="PLG42_07695"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOU31130.1"
                     /translation="GLLERIGLNDSIFTHKPTGKEQYRIITDIPDHTVGINMVIDALI
                     DHEHGVIKSIDEITAVGHRVVQGGEKFQKSVLIDEDVKKAIEIYCELAPLHNPANLKG
                     ILSVEKLLPHVPQVAVFDTSFHQTMPDYAYMYAIPYEYYEKYRIRKYGYHGTSHKFVA
                     SKAAGILGRDIKELKIITCHLGNGSSMTAIKNGISIDTSMGFTPVDGLIMGTRTGDID
                     AGVLLYLADKEHLNLAGINNLINKKSGVLGISGISSDMRDLEQAAADGNPRAILALKM
                     FAYRVKKYIGAYTAAMNGLDLLIFTGGIGENDFNMRKLICTGMEYIGIVFNEEVNEGA
                     RGKDLILSKPESRVTVMSITTDEEFVIASDTKSIVEKL"
     gene            1145..2053
                     /locus_tag="PLG42_07700"
     CDS             1145..2053
                     /locus_tag="PLG42_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOU31131.1"
                     /translation="MLKKLDEIRTIIANEPVKKLVLAAANDHDSLSAVLRAAKDGIVI
                     PILVGDKEAIQNIASSYGYDISDLRIVHEPDTEKAVEIAVKMASSKQAEILMKGKVGT
                     STLLKSVLNREWGLRTGKLLSHFALFEVETYHKLIAVTDVAMNIAPNLQEKIAIINNS
                     IDCLHKLGIALPKVAVLGAVEMVNENMSATLDAALLSKMNQRDQIKGCIIDGPLAFDN
                     AVSVESARHKGIKSEVAGDTDLLLMPDIEVGNVLYKSLVFFAKAKVASVILGAAVPIV
                     LTSRSDSEQAKYDSIMLATAVSRKQQ"
     gene            2050..2964
                     /locus_tag="PLG42_07705"
     CDS             2050..2964
                     /locus_tag="PLG42_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOU31132.1"
                     /translation="MIRTLDEMVDVVLSFRKKHRLAVAWAHDNNTIEAVGNAVINGLA
                     EAVLIGHKKEIIRTCEAESIDYSIFTITESKNEITASQEAVSMVKTGEADIVMKGLVS
                     TDKFLKAVMDKENGIMKPGAVLSYVCAVQIPSYHKLLFITDPAVIPFPDLDQKIAMAE
                     YAIEMANTFGIEKPRVALIGASEKISRHSENSIHYAVMCKMAERGQIKNCIMDGPLDI
                     FLACDRKSQELKQVDTPICGDADILLFPSLESCNPFYKGLMLFAGGELAGLIRGTSKP
                     VVLMSRSEKKKSKYYCIALACLLAERNI"
     gene            2986..4061
                     /gene="buk"
                     /locus_tag="PLG42_07710"
                     /pseudo
     CDS             2986..4061
                     /gene="buk"
                     /locus_tag="PLG42_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
     gene            complement(4094..4789)
                     /locus_tag="PLG42_07715"
     CDS             complement(4094..4789)
                     /locus_tag="PLG42_07715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOU31133.1"
                     /translation="MNIIITGGSSGIGLETAKVLSENKEINIIVTGRNETALKKVSDY
                     SPYHNIIPLITDFTTLASNSESILKFVRKYFDKIDILINNAGYLYARKFDETDEKEAR
                     LMMEINFFVPAMLIRLFLPLFHRGSHIVNISSMGGYQGSQKFPGLSYYSASKAAIACL
                     TECLATELIDREIYVNCIAPGSVQTEMFDRAFPGQKANLSAEEMGKFLAWFAINGHRY
                     FNGKIIPVAMVSP"
     gene            4863..>5356
                     /locus_tag="PLG42_07720"
     CDS             4863..>5356
                     /locus_tag="PLG42_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31134.1"
                     /translation="MKRGRKVVAGILLTLFFSLYTADKLIAQDKNNERPPFKERLFWG
                     GSFSLQLGTLTDIEIAPVVGVWLLPRLAIAGGPNYRFYKFLGEQTDIYGIQSYLQVVL
                     LRDIDKLIPIGSHTSIVLQVDNELLSLESSFWKGSSYIPGESKRFLVNTPLIGGGLSQ
                     QIGR"
BASE COUNT         1701 a          949 c         1170 g         1536 t
ORIGIN      
        1 aaggccttct ggaaagaata ggactaaacg attcaatctt tactcataaa cccaccggca
       61 aagagcaata cagaattatt actgatattc ctgaccatac tgtcggaata aatatggtga
      121 ttgatgcact cattgatcat gagcatggag tgattaaaag cattgatgaa attacggcag
      181 tagggcaccg ggtggtacag ggcggcgaaa aatttcagaa aagcgtttta attgacgaag
      241 atgttaaaaa agctatagag atatattgtg aacttgctcc gctccataac ccggcaaatc
      301 tgaaaggtat cctttcagtt gaaaaactgt tacctcatgt tccccaggtt gcagtgtttg
      361 atacatcctt ccatcaaacc atgccagatt atgcttatat gtatgctatt ccgtatgagt
      421 actatgagaa atacagaata agaaaatacg gatatcatgg aaccagtcac aaatttgttg
      481 ccagtaaagc ggccggtatt ctcggcaggg atataaaaga gcttaaaatc attacctgcc
      541 accttggaaa tggctcatca atgaccgcca taaaaaatgg aatctcaata gatacctcta
      601 tgggtttcac cccggttgac ggactgatta tgggaacccg tacaggagat atcgatgcag
      661 gtgtgcttct gtatctagct gataaagaac atcttaatct tgcaggtatt aacaatctga
      721 taaataaaaa aagcggcgtt ctcggaattt caggaatatc ttcagatatg agagatctgg
      781 agcaagctgc agcagacggt aatccgaggg caatactggc tttaaaaatg tttgcctaca
      841 gagttaaaaa gtatattggt gcttatactg cagcaatgaa tggccttgac cttttaatat
      901 ttaccggggg aatcggtgag aatgatttca atatgagaaa gttgatctgc accggtatgg
      961 aatatattgg aatagtgttc aatgaagaag taaatgaagg agcgagaggg aaagacctga
     1021 tattgtcaaa acctgaatct agagttaccg tgatgtcaat aactacagat gaagaatttg
     1081 taatagcatc tgatacaaaa agtattgtgg aaaaattata aaagccttta gtaattatca
     1141 ggtcatgttg aaaaagcttg atgaaattag aacaataatt gcaaatgagc ctgttaaaaa
     1201 actggttcta gcagcagcca atgatcatga ttcacttagt gcagttttac gagctgcaaa
     1261 agatggtatc gtaattccga ttcttgtggg cgacaaggaa gctatacaaa atatagcatc
     1321 atcatatgga tatgatatat ctgacctgcg aatagttcat gaaccagaca ccgaaaaggc
     1381 agttgagata gctgtaaaaa tggcaagcag caaacaggct gaaatactta tgaaagggaa
     1441 ggtcggtaca tcaacgctgc tgaaaagtgt tctcaacagg gaatggggac tacgtacagg
     1501 caaattgctc tcacattttg ccctgtttga agttgagaca taccacaaac tgattgcagt
     1561 tactgatgtg gcaatgaaca tagcaccaaa tcttcaggaa aaaattgcaa ttattaataa
     1621 ttcgattgat tgtctgcaca aactgggtat agcacttcca aaagtggcag ttctgggagc
     1681 tgtagaaatg gtcaatgaaa atatgtcagc cacactcgat gctgctttgc tttcaaaaat
     1741 gaaccagaga gaccagataa aaggctgtat tattgatggt ccactggctt ttgataatgc
     1801 agtaagtgtt gaaagtgcgc gtcataaggg tattaaaagc gaggtggcag gcgatactga
     1861 ccttttgctt atgcctgata ttgaggtggg taatgtccta tataaatcac tggtattctt
     1921 cgccaaagca aaagtggctt cagtaatatt aggagctgct gttcctattg tgcttacatc
     1981 acgttccgat tcggagcagg ccaaatacga cagtataatg cttgcaaccg cagttagcag
     2041 gaagcaacaa tgataagaac cctcgatgaa atggtcgatg tggtgctttc ttttcgaaaa
     2101 aagcaccggc tggctgttgc ctgggcacat gataataata ccattgaggc tgtgggcaat
     2161 gctgtaatta acggacttgc agaagcagtt ctgataggtc acaaaaaaga aattattaga
     2221 acatgcgaag ctgaatcaat cgactattca atattcacga ttactgaatc gaaaaacgaa
     2281 atcaccgctt cacaggaagc agtttccatg gttaaaacag gagaagccga catagtcatg
     2341 aaaggattgg tcagcaccga taaattcctt aaggctgtga tggacaagga aaacggaatt
     2401 atgaaacctg gtgcagttct ttcatatgta tgtgctgtac agattccatc gtaccataag
     2461 cttcttttta ttaccgaccc tgcagtaatc ccttttccag accttgacca gaaaattgcc
     2521 atggcagaat atgccattga aatggcaaat actttcggaa tagaaaaacc cagggtggca
     2581 ttgatcggag catcagagaa aatctccagg cactcggaaa attctattca ttacgcagtt
     2641 atgtgtaaaa tggctgaacg aggtcagatt aaaaactgca ttatggatgg tccgctggat
     2701 atttttctcg cctgtgacag gaaaagccag gagttgaaac aggtcgatac tcctatttgt
     2761 ggtgatgctg atattcttct ttttccttcg cttgaatcat gcaatccgtt ttataaagga
     2821 cttatgcttt ttgcgggagg tgaactggcc ggtcttataa gaggcacatc aaaacctgtt
     2881 gtattaatgt cgagaagtga aaaaaagaag tcaaaatact attgcatagc ccttgcttgc
     2941 ctgctggctg aaagaaatat ataaacaaat agttcagcta ttggaatgaa aaaatcactg
     3001 attattgcaa ttaatccagg ttctacttcc actaaaattg ctgtatttga agaggaaaac
     3061 cttctttttg aaaaaacaat cagacattca acagaagagc ttcaaagatt ttccaggata
     3121 actgaccagt tccattttcg taaagatctg attatgaaag aactggaaca ttataaaatt
     3181 gatttttcaa gggttgcagc agttgtagga aggggtgggc ttgtaaagcc tattgaatcg
     3241 ggagtttaca gggtgaatga gagaatgaaa aaagatctga gagaaagtcc aatgggagag
     3301 catgccagca atctaggagc tcttattgcg gatgatatag cttctgaatt acaagatgtt
     3361 cctgcattta ttgttgatcc tgttgttgta gatgaaatgg aacctgttgc cagactatca
     3421 ggtcatcctg aaattaaacg gctctcgatt ttccatgcgc tcaaccagaa ggcggtcgca
     3481 aggctttatg cagcctctaa gggaaaagag tatgaaaagt tgaatcttat agttgttcac
     3541 atgggaggtg gaataagtgt aggagctcac aagcaggggc gggtagttga tgtcaacaat
     3601 gcactaggtg gagatggacc tttctctccg gaacggtcag gaggactccc ttcaaaacaa
     3661 ttggtcgatc tctgcttcag tggtaaatat acttacgagc agattaattc aatgcttatc
     3721 ggcaaaggcg ggatggtagc atacctcgga acaaatgatt tcagagatat ctccaggagg
     3781 gctgaagaag gagatgagtc agcattgctg gttatagatg cagctgcgta ccagatttca
     3841 aaggaatagg cagtatggct gcagttctcg aaggaaaagt tgattcaata attcttacgg
     3901 gtggaatggc ttatcaaaaa tcattcaatg aaaaaataat atcccgtgta agctttattg
     3961 ctgaggttgt aatatatcct ggtgaagatg aaatgaaagc acttgctttt aatgtgattc
     4021 ttgctttacg gggtcaacta cagataaagg aatacttctg ataaaccaag ataacatcgt
     4081 gtttttaaaa tatttatggt gataccattg caaccggtat tattttgccg ttgaaatatc
     4141 tgtggccgtt gatagcaaac catgccagga atttgcccat ttcttcagcg cttaaatttg
     4201 ccttttgacc tggaaaagct ctgtcaaaca tttctgtctg aacactcccc ggtgcaatac
     4261 agttaacata aatttccctg tctatcaact ctgttgcaag gcattcagtt aaacatgcta
     4321 ttgctgcttt ggaggcacta taatatgaga ggcccggaaa tttctgacta ccctggtacc
     4381 cacccatact tgagatattt actatatgag aacctctgtg aaacaaagga agaaaaagac
     4441 gtatcagcat tgcggggaca aaaaaattta tctccatcat taatctggcc tctttttcat
     4501 ctgtttcatc aaattttctg gcatacaaat atccggcatt attaataagt atatcgattt
     4561 tgtcaaaata ttttcgtaca aattttaata ttgattctga attacttgcg agagttgtga
     4621 aatctgttat tagagggata atattgtggt aaggcgaata atccgaaact tttttaagag
     4681 ccgtttcatt tctacctgtt acaattatgt tgatttcttt gttttcggat aaaactttcg
     4741 ctgtttctaa gccaattccg ctagagcctc cggtaataat gatgttcata tttaaagttt
     4801 ctttactgga taaaagagtt ttttcgttta tttgtatgta tcaaatatac aacttttaag
     4861 aaatgaagag aggcaggaaa gtcgtcgctg gaattttgct tacattattt tttagtctat
     4921 atactgctga taaactgatt gctcaggata aaaataatga aagacctccc tttaaggaga
     4981 ggttattctg gggtggaagc ttttcccttc agctggggac tcttacagat attgagatag
     5041 cccccgtggt cggagtctgg cttttaccac gtctggcaat tgctggagga ccaaattaca
     5101 gattttacaa atttctcgga gagcaaactg atatatacgg aattcaatct tatttacagg
     5161 tagtgctcct gcgagacatt gataaattaa ttccaatcgg atctcatacc agcattgtgc
     5221 ttcaagtcga taatgagctc ctttcccttg aatcgagttt ctggaaagga tcctcttata
     5281 tcccgggtga atcaaaaaga tttcttgtga acacacctct tataggtggc ggactcagtc
     5341 agcagattgg aagaag
//
LOCUS       DAOSNB010000179         5321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15560_length_5321_cov_5.090771, whole genome shotgun sequence.
ACCESSION   DAOSNB010000179 DAOSNB010000000
VERSION     DAOSNB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15560_length_5321_cov_5.090771"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLG42_07725"
     CDS             <1..760
                     /locus_tag="PLG42_07725"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU31135.1"
                     /translation="TIISFNKDVDSGSYQQFASVPRPGHADFTSRIKYSGFADPRGGG
                     HFSGRITWGLVVAGVIAKKILKDSKVIATLIEAGGSKDIERAVEEAMNAGDSIGGLIE
                     CRVENPPAGLGEPFFYSAESAISQILFSIPAVKGVEFGAGFKAASMKGSEHNDPFTDS
                     TGKTLTNNAGGINGGITNGNEILLRVAVKPTSSIGKEQKTFNFSAGKMTTLKVEGRHD
                     ACIALRVPVIVEAAVAVALADLLLLDRGIFSRKD"
     gene            complement(757..2097)
                     /gene="tilS"
                     /locus_tag="PLG42_07730"
     CDS             complement(757..2097)
                     /gene="tilS"
                     /locus_tag="PLG42_07730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOU31136.1"
                     /translation="MLEDFLNYIEKNSLAEKSERILLAVSGGIDSMVMADLFSKTGYF
                     TGIAHCNFRLRGTDSDNDELFVKEYAARLNKPFFSIGFNTAEYAREKGISTEMAAREL
                     RYRWFEEIRCKYGYNKIALAHNRNDNVETILLNLCRGTGIKGLTGMKPLNGNLIRPLL
                     FASRSQIQNYASENSIEYRLDITNDEVKFIRNKFRHQIIPLFREINPSFDDTVNEMAE
                     RFSEIREILENHIRTVKQNVVIKENGETVLSLQKLLEYGPQKTILFELFQPYGLLASQ
                     LDTLIKVINGQTGSLIFTETHRIIKNRDEIIITKLPVPQSKDFFISNIEDFYRLPFIE
                     SAELKQVTSDFRIEHDPHVATVDASLLEFPLKIRSWKAGDRFMPFGMESFRKLSDYFT
                     DRKFSLPDKEKALVMESAGKIVWLLGERIDNRFRITSSTRNALIIRLRKNFRVF"
     gene            complement(2146..4092)
                     /locus_tag="PLG42_07735"
     CDS             complement(2146..4092)
                     /locus_tag="PLG42_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31137.1"
                     /translation="MCLREIRKKCDTIRPFLQVLLILLTSVVYPQAETGRDTTLLKIK
                     SGSFLRINNDRFFIRNDSVIVLPSFFVNVNRTKNEKTIAFYDSLKSKASRTRLTRVLY
                     DLVVVSPDTVNPEKINQRSDESFIKYSGYRVRSITIKQLDVFGGNIYNPAVSGANRIE
                     KFINSTHINTNEKVLRRYLLFSEGDTISPLTITDNERILRQLPYINDARIIVAPVSDN
                     EADVIIITKDVYSLGADFTYRGINKGSVWIYDKNLIGTGQELKIEIPYSATTKDSPGI
                     GINYNITNILKSFIDLNLNFYKGLGKKSFGFDIDRPLLSSETKYAFGISVNNTYTTHD
                     LDTLPVPEPLKYNFQDYWLMRSFMIDRRTVSRLIAGIRYVNNNVFQKPDIQPDTYYSL
                     QRYRLYLASLAFSMQKYYKTSLLYSFGRTEDIPYGTLINITSGIEDNEFKKRVYFGSN
                     ASLGFSVSSLGYFHLSAGAGTFFNDNKTEQGVVSYQLKFFSNLIPIGKQMIRNFVHLR
                     YTGGFDRYRDEYLTTLKEDGFSCFANDSLRGTRRFNAGIESVIFNPINLYGFRFAFFT
                     FVDLYAMGGLNFNSRNSLMLSGLGVGLRIRNDNLVFRTFQIRIGYFPYLPEYSRVNYI
                     MLSGEQLLRPKNFEPGRPSLIQYR"
     gene            complement(4092..4802)
                     /locus_tag="PLG42_07740"
     CDS             complement(4092..4802)
                     /locus_tag="PLG42_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31138.1"
                     /translation="MPEAEDKKNNIVNTLKSKSVLKQKVYDITFSTFTLLKEILKDLT
                     KEINLDIAGIDQRVKLEFTDRGQFDAQIKVAGDILLFSMHSNIFQFDRDHPVWRTPYV
                     QKDKFNAYAGIIYIYNFLADSFKYNRLDDLGYLIARIFINHEKQYFVEGKRQMGMFYT
                     NFGNEEINRESLQRIILTAIQYSLEFDLLVPPYDTVKIATVGQAEAKIQHSKIITGKR
                     VGFQFNSDDVLVANTNKP"
     gene            complement(4914..>5321)
                     /locus_tag="PLG42_07745"
     CDS             complement(4914..>5321)
                     /locus_tag="PLG42_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOU31139.1"
                     /translation="VPVRIAVGPRDVKNNTAEIARRDTLEKETIPADRIKEHVVRLLE
                     DIQKNIYRKALQFREENTYYPDSWEEFKNIIEDKGGFVMAHWDGTPETEARIKEETKA
                     TIRCIPIDSKEEKGKCIYSGKPSGRRVLFAVAY"
BASE COUNT         1498 a         1068 c         1083 g         1672 t
ORIGIN      
        1 tactataatt tctttcaata aggatgttga ctccggctcg taccagcagt ttgcttcggt
       61 tccacgtccg ggtcatgctg attttacatc cagaattaaa tactcaggat ttgcggatcc
      121 gagaggcgga ggccatttct cgggcagaat cacatggggt cttgttgttg ccggagttat
      181 cgctaaaaag attctaaaag attcaaaagt gattgccaca ctaattgaag caggcggcag
      241 taaagatatt gaaagagctg tcgaagaagc aatgaatgct ggcgacagca taggcggact
      301 gatagagtgc agggtcgaaa atccgccagc aggactgggt gaaccattct tctattctgc
      361 tgaatcggct ataagtcaga tactattttc aatccctgca gttaagggag tagaattcgg
      421 agcaggcttc aaagctgcct caatgaaagg ctcggaacat aacgaccctt ttaccgatag
      481 caccggcaaa acacttacaa acaacgccgg gggaattaac ggcggcataa caaacggtaa
      541 tgaaatatta ctgagggtgg ctgttaaacc tacatcatca attggaaagg aacaaaaaac
      601 atttaatttt tcagcaggca aaatgacaac cctgaaggtg gaaggccggc acgatgcctg
      661 cattgcactg agagtgcctg tgatagtgga agcagctgta gctgttgccc ttgctgacct
      721 cttactgctc gacaggggta tttttagccg aaaagattaa aaaactctga agtttttcct
      781 gagtctgatt atcagggcat tcctggtgga agatgtaatc ctgaaacggt tgtcaattct
      841 ttctcccagc agccatacta tctttcctgc cgactccata accagagctt tttctttatc
      901 aggaagagaa aactttcggt cggtgaaata atcgcttagc ttcctgaacg attccatacc
      961 gaaaggcata aacctgtcac cagccttcca gcttcttatc tttaaaggga attctaacaa
     1021 tgaagcatca acagttgcaa catgtggatc atgttcaatt ctgaaatcgg aagtcacctg
     1081 cttcagttca gcactttcta taaaaggtaa cctgtaaaaa tcttctatat tactgataaa
     1141 gaaatcttta ctttgcggta ccggcagttt tgtaattata atttcatcac ggtttttgat
     1201 tattctatgt gtttcagtaa atatcaaaga gcctgtctgt ccgttaatta cttttatcag
     1261 ggtgtccagc tgactcgcca gcagaccata aggttgaaaa agttcaaaga ggatagtttt
     1321 ctgagggccg tattccagaa gtttttgtaa gcttaaaact gtttctccgt tttccttaat
     1381 gacaacattc tgtttaactg ttctgatatg attttcgaga atttccctta tctccgagaa
     1441 acgctcagcc atttcattta cagtatcgtc gaatgatggg ttaatttccc tgaacagcgg
     1501 aataatctga tgcctgaatt tatttcttat gaattttacc tcatcgttag ttatgtcgag
     1561 gcggtattca attgagtttt cagaagcata gttctgaatt tgcgaacgtg aggcaaacag
     1621 aaggggacgt ataagatttc cgttcaaagg cttcattcct gtaagtcctt tgattccggt
     1681 tcccctgcat aggtttaaaa gtattgtttc aacattatcg tttctgttat gagccagggc
     1741 tattttattg tagccgtatt tacatctaat ctcctcaaac catctgtatc tgagctccct
     1801 tgctgccatc tctgttgata ttcctttttc cctggcatat tcagctgtat taaaaccaat
     1861 actgaaaaat ggcttattca accgggcagc atattccttt acaaacagct catcattatc
     1921 tgagtctgtt cctctcagcc tgaaattgca gtgtgctata ccggtgaaat acccggtctt
     1981 tgaaaataga tctgccatca ccattgagtc gattccaccg cttacggcaa gaagtatcct
     2041 ttcggatttt tccgcaagtg agtttttctc tatataatta agaaaatctt ccaacatctg
     2101 gctgaaagta aaactgtgaa aataataatt ttctgtatag gaaaattacc tgtattgaat
     2161 tagtgaaggc cgtccgggct caaaattttt tggtctcagc agctgttctc cggaaagcat
     2221 tatataattt actctggaat attccggcag ataaggaaaa tatccaatgc gtatctggaa
     2281 cgttctgaaa accagattgt catttcttat cctcagacct actcccaatc cgctaagcat
     2341 caggctgttt ctggaattaa aatttaaccc tcccatagca tacaaatcga cgaaggtgaa
     2401 aaatgcaaat cggaaaccgt ataaattaat cggattaaat attactgatt caattcctgc
     2461 attgaaccgt ctggttccac ggagggagtc attcgcaaag cacgaaaatc catcttcctt
     2521 cagggtggtg agatactcat ccctgtaacg gtcgaagcca ccggtgtatc tcaggtgaac
     2581 gaaatttctg atcatctgtt taccgatcgg aatgaggttt gaaaaaaact tcagctggta
     2641 tgaaactact ccctgttcag ttttattatc attgaaaaag gttcctgcac cagctgaaag
     2701 atggaaataa cccagactgc tgactgaaaa tccaagagaa gcattggaac caaaatagac
     2761 acgttttttg aattcattat cttcaatacc ggaggtgata tttatgagtg ttccataggg
     2821 gatatcctct gttcgaccga aactgtaaag gaggctggtt ttataatatt tctgcattga
     2881 aaatgccagg gatgcaaggt atagcctgta acgctgcagt gaataatatg tgtcgggttg
     2941 tatatcaggt ttctggaata cattattatt gacatagcgt attcctgcaa taagtctgga
     3001 aacagtgcgg cggtcaatca taaaggaacg catcagccag taatcctgaa aattgtattt
     3061 taagggttcg ggaacaggta gagtatcaag atcgtgtgtt gtataagtgt tatttactga
     3121 aattccgaaa gcatattttg tttcagaact taaaagaggc cggtcaatgt cgaatccgaa
     3181 tgattttttg ccaagtcctt tgtaaaaatt gaggttaaga tctataaaag atttcagaat
     3241 gttggtaatg ttatagttta ttccgattcc cggagaatct tttgttgttg ccgaataagg
     3301 tatttcaatt ttaagttcct gccctgttcc aataagattc ttatcataaa tccaaacgga
     3361 acctttgtta attccccggt acgtgaaatc agcaccaaga gaatatacat ccttggtaat
     3421 aataataaca tcagcctcat tgtcagagac aggtgccaca attattctgg catcgttaat
     3481 gtaagggagc tggcggagaa tacgctcatt atcggtgatt gtcagagggg agatggtatc
     3541 cccttcactg aaaagcaggt atcttctaag aaccttttcg tttgtgttta tatgggtgct
     3601 gttgataaat ttttcaatcc ggtttgcacc tgaaacagcc ggattgtaga tatttccacc
     3661 gaaaacgtcc agctgtttaa tggttatgct cctcaccctg tagcccgagt actttatgaa
     3721 actctcatca cttctctggt ttattttttc gggatttaca gtatcgggtg atactacaac
     3781 cagatcgtac agaaccctgg ttaatctggt tcgtgaggct tttgacttca gggagtcgta
     3841 gaaagcaatt gtcttttcgt tttttgtcct gttcacatta acgaagaagg aaggcaggac
     3901 aattacggaa tcatttctga taaaaaacct gtcgttattt atgcgcagaa atgaaccgct
     3961 cttgattttt aataaagtgg tatcacggcc agtttctgcc tgtggataaa caaccgatgt
     4021 taacaatatc agcagaacct gcaggaatgg cctgatagta tcacactttt ttcttatctc
     4081 ccgaaggcac atcagggttt gttggtattg gctacaagta catcgtcaga attgaactgg
     4141 aagcccacac gttttcctgt aataattttg gaatgctgaa ttttagcttc ggcctgacca
     4201 acagttgcga tttttactgt gtcgtaaggc ggcaccagca gatcaaattc aagagaatac
     4261 tgtatagcag taagaattat cctttgcagt gattccctgt ttatctcttc attgccgaag
     4321 ttcgtgtaaa acatacccat ctgacgcttt ccttcaacaa aatattgctt ttcgtggttt
     4381 atgaaaatcc tggcaataag gtatccaagg tcgtcgaggc ggttatattt gaaactgtca
     4441 gcaagaaagt tataaatata tataatgcct gcataggcat taaacttgtc tttctggacg
     4501 tatggtgttc tccatactgg atgatccctg tcgaactgga aaatgtttga atgcatgctg
     4561 aacagaagta tatcgcccgc aaccttaatc tgtgcatcaa actgccccct gtcggtgaat
     4621 tcaagtttta cccgctggtc aattcctgct atatcgaggt taatttcctt tgtgaggtcc
     4681 ttcagtattt ccttcaggag ggtaaaagta ctgaatgtaa tgtcatagac tttttgctta
     4741 agcaccgact tgctttttaa tgtattgaca atgttgtttt ttttatcttc agcttcaggc
     4801 attgttaaga gttttataac acaatgaaca actaaaatag gaaattaatc cgatatataa
     4861 aatcggttgg gtattctttg tacgtttgaa aaaaaacgta taaccacgcc ggctcagtat
     4921 gctacggcga aaagaacacg cctgccagaa ggtttacctg aatatatgca ttttcctttt
     4981 tcttccttgc tgtcaatagg aatgcatctg atggttgctt tggtttcttc ctttattctg
     5041 gcttcagttt cgggagttcc gtcccagtga gccataacaa agcctccttt atcttcaatt
     5101 atgtttttaa attcttccca tgaatcggga tagtatgtat tttcttccct gaactgaagg
     5161 gcttttctgt aaatgttttt ctgtatatct tcaagcagcc gcaccacgtg ttcttttatt
     5221 ctgtcggccg ggattgtctc tttttcaaga gtgtctcttc tggcaatctc agcagtattg
     5281 ttcttgacat ctctcggacc aacagctatt ctcacaggta c
//
LOCUS       DAOSNB010000180         5244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15866_length_5244_cov_5.213336, whole genome shotgun sequence.
ACCESSION   DAOSNB010000180 DAOSNB010000000
VERSION     DAOSNB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15866_length_5244_cov_5.213336"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLG42_07750"
     CDS             <1..444
                     /locus_tag="PLG42_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOU31140.1"
                     /translation="EIREQNGKVILNHPFKGHDLEKIDISKVDFIEAYNARLTEEENK
                     KAVELAMKYNKQVVAGSDAHLYGEVGNAYTVFDNFNSFIPLRHHYIPSKHIYKTISQY
                     IKSFKERNFRIFFSATAIQLKHSGKIALGGLKKFFRFLNNSKTAA"
     gene            453..1493
                     /locus_tag="PLG42_07755"
     CDS             453..1493
                     /locus_tag="PLG42_07755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU31141.1"
                     /translation="MEEIRPGSMLILTDGNIDQASARVRAISYIPLFESEGFKVVHIP
                     RVPCRPASFFQKYFSFPVTKRLLWLKRIFALLTGKWNIIYIQRLLISEWLMRIVIRKS
                     FVIFDFDDAIYLPEKPGRRSHKTGIMINYADKVIVSTEYLNDFVGRYGKKAIVIPSPV
                     ETDRIFPMQSDHTREIPVIGWIGSYWTTGYIKIIEPALQKLAEKTHFTFLTVGSDPAY
                     KIAGINHVVKPWSLENECSLVNEMDIGIMPLPDDEFTRAKGGYKLYLYMAAGIPCVAS
                     PVGINSSIIRHGENGFLASSENEWIEALSKLLSDIELRKKMGSKGREDALNFYDRKVC
                     FSMIMNLVNQTK"
     gene            1490..2491
                     /locus_tag="PLG42_07760"
     CDS             1490..2491
                     /locus_tag="PLG42_07760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU31142.1"
                     /translation="MNFENNLKGADKYQISEPKCRICGNSKGNKIFTAKERQFGTLEE
                     FNYFTCSSCGCIQILEFPKNPGKYYPADYYAYDQPVFPTKLTGIIAYLKKSLLRYYIG
                     YFNIPGFILSLVYKHPFPWIRKKEINFNSRILDVGCGSGRKLLSLYRSGFRNLTGIDP
                     YIEKDIIYPEGVIIRKMELSDMQGEFDFIMLHHSFEHMPDPKNVLYHINRLLAPEGSV
                     LIRIPVADSASCKKYGEYWSGLDAPRHFFIHTRKSIEILAENTGFFIDGLSYDSTRDQ
                     FVTSEKYLRGLPKNSPDSIFSRSELKKFDREAEKLNKLMEGDWACFYLKKKTDKKYE"
     gene            2484..3716
                     /locus_tag="PLG42_07765"
     CDS             2484..3716
                     /locus_tag="PLG42_07765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU31143.1"
                     /translation="MSKTLILFTDRYPFGKAETFLENEIKYFASAFTRVVIFPLQRGD
                     DDYVRNIPENALLEQPVFKRIKKSPELIFKGIFNTSCLKKFFIEGFKSGALHSPSRFR
                     IWITHLLMIRAILSYIKKNNLIAFFNEADVLFFNWGLRWSQVIPFLPENLRTRIAVRF
                     HGSDLYEHLNSDYIPWRREQIKRIDSVITVSEAGKNYLSTKYAIPKENIFLSRIGTPD
                     FGLNPYEKTETVRIVSCSNIVPVKRVELIALALKYLKLKVEWIHFGEGPLKQKVTELA
                     MQLPGNISWQFPGEINHEKLMNFYKTVSVDLFLNVSSSEGVPISVMEALSFGIPVIAT
                     NAGGTSEIVTEPGGVIIPVEFEPSFLAEKIENIVNAENYLQMRQAARKTWEEKCREDK
                     LYPLLIEFLLNNPAENKK"
     gene            3795..>5244
                     /locus_tag="PLG42_07770"
     CDS             3795..>5244
                     /locus_tag="PLG42_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31144.1"
                     /translation="MQNSINIKATLPHAVAIILFAVVSLVYFYPVIEGKVLVTNDGTV
                     AYNSASEIRNFREKYGQEPLWTNSMFGGMPAYLISVKYYGNLMRYVDQFLKFMKLPVA
                     PVFLTMTGFYILLLFFKVDYRLAIAGAIAYGFSSYFIIILGAGHNTKAFAIAYMAPVI
                     GSVYYSYKKDLIKGSLLLAFFLTLQILSNHPQILYYTLLCILVFIIAEFITAIRTKEV
                     IPFLKKSLILIIPVLLSAGMNFASLYTTYEYGKYSIRGKSDLIIPGQREVKGLNKDYA
                     TQWSYGIDETLTMLIPNFKGGANRPFDTGSATAKALRLNNASQYAANFHRYWGSQPWT
                     DGPVYVGAIVVFLFIMGLILIKGPIKWWLAIATLLSVMLSWGKNFMPLTDLFFYYFPG
                     YNKFRAVTMILVIAEFCMPLLAILTLKEIFEKRISQKDFLKALKISLGITGGLTLLFL
                     LFPGLAGSFLSPGERESQLPGWLTEALKKDRIE"
BASE COUNT         1710 a         1005 c          998 g         1531 t
ORIGIN      
        1 gaaatcaggg aacagaacgg gaaggtaatt ctgaaccatc cttttaaagg gcacgatctt
       61 gagaaaatag acatttctaa agttgatttt atagaggctt acaacgcacg gctcacagaa
      121 gaggaaaaca aaaaagctgt cgaactcgca atgaaataca acaaacaggt agttgcagga
      181 agtgatgccc atctttacgg ggaagtaggc aatgcctata cagtgtttga taattttaac
      241 agtttcatac cattacggca ccattatatc ccatcaaaac atatatataa aacaattagc
      301 cagtacatta aatcattcaa agaaagaaat ttcaggatat ttttttcggc aacagcaatt
      361 cagttgaaac attcaggtaa aatagctctg ggagggctga agaagttttt tcgattcctc
      421 aataactcta aaactgctgc atgaataaaa aaatggaaga aataagaccg ggcagcatgc
      481 tgattcttac cgacggcaat atcgaccagg cgagtgcaag agtcagggct atttcataca
      541 tccccctttt tgaatccgaa gggttcaaag tggttcatat tccccgtgtt ccctgcaggc
      601 ctgcttcctt ttttcagaaa tatttttcat ttcctgtaac taaacgatta ttatggctta
      661 aaagaatttt tgccctactc accggcaaat ggaatattat ctatatacag agacttttaa
      721 tcagtgaatg gttaatgcgt atagtaatcc ggaaatcgtt tgtgattttc gattttgacg
      781 atgctatata tcttcctgaa aagccgggcc gcagaagtca taaaacaggc ataatgatta
      841 actatgccga taaagtcatt gtaagtactg aatatctgaa tgactttgtg ggcaggtatg
      901 gcaaaaaggc aatcgtcatc ccttcaccgg ttgaaacaga cagaattttc cccatgcaaa
      961 gtgaccatac aagggaaata ccagtaatag ggtggatagg atcatactgg acaacaggat
     1021 acattaaaat aatcgaacct gccttacaaa aactcgcaga aaagactcat tttacttttc
     1081 ttaccgttgg atcagatcct gcatataaaa ttgccggtat taaccatgta gttaaaccat
     1141 ggtcgctgga aaatgagtgt tccctggtga atgaaatgga tattggtata atgcctctac
     1201 cggatgatga gtttacacgt gcaaagggag gatataaact ttatctttat atggctgcag
     1261 gcattccttg cgtggcaagt ccggtaggaa tcaacagttc aatcatcagg catggagaaa
     1321 acggttttct tgcctcatcg gaaaacgaat ggattgaggc cctcagcaaa cttctgtccg
     1381 acattgaact gagaaaaaaa atgggaagca aaggcaggga agacgcttta aacttttacg
     1441 acagaaaagt atgcttttca atgattatga accttgtcaa ccaaacaaaa tgaattttga
     1501 aaataatctg aaaggcgcag ataaatatca aatatctgaa ccaaagtgca gaatttgcgg
     1561 aaacagtaag gggaataaaa tcttcaccgc taaagaaagg caattcggca ctctggaaga
     1621 attcaactac ttcacctgct catcctgtgg ttgtattcag atacttgagt ttccgaaaaa
     1681 tcccggaaaa tattatcctg ctgactatta tgcttacgac cagccagttt ttccaacaaa
     1741 acttacaggc attatagcat atctaaaaaa aagcctcctt cgatactaca ttggatattt
     1801 caatattcca ggattcatac tctcccttgt atataaacac ccttttccgt ggatcagaaa
     1861 aaaagaaatt aactttaact ccagaatact tgatgttggg tgtggatcag gcagaaaact
     1921 tctttcactt tacagaagcg gattcagaaa tttaacaggg atcgatccct atattgaaaa
     1981 agatatcatt tatccagaag gagtaataat cagaaaaatg gaattatctg atatgcaggg
     2041 ggaatttgat ttcataatgc ttcatcattc ttttgaacac atgcctgacc caaaaaatgt
     2101 tctctatcat ataaacagac tgcttgctcc ggagggtagt gtattaatac gcattcctgt
     2161 tgctgattca gcttcatgca agaaatacgg agaatactgg tcgggactcg atgcgccccg
     2221 tcatttcttc atacatacca gaaaaagtat agaaatcctc gctgaaaata ccggattctt
     2281 tatcgatggc ctgtcatatg actccacccg tgaccagttt gttacaagcg aaaaatatct
     2341 cagaggcctg cctaaaaata gcccggattc aattttcagc aggtcagaac taaaaaaatt
     2401 cgaccgggaa gccgaaaaat taaacaaatt aatggaaggc gactgggcat gtttttatct
     2461 gaagaaaaaa acagataaaa aatatgagta aaactctgat attatttacc gacagatatc
     2521 cgttcgggaa agccgaaacc tttctggaaa acgaaatcaa gtatttcgca agcgctttta
     2581 ccagggtggt tatttttccg cttcagcgtg gcgatgatga ttatgtgagg aatatccctg
     2641 aaaacgcact tctagaacaa cctgttttta aaagaataaa aaagtcgcct gaacttattt
     2701 tcaaaggtat tttcaatact tcttgcctga aaaaattttt tatagaaggc tttaaatccg
     2761 gtgcattgca ctctccttcc agattcagaa tctggataac acatcttctg atgattcgtg
     2821 caattctttc atatattaag aaaaataatc ttatagcttt ttttaatgaa gccgatgtgc
     2881 ttttctttaa ctggggcctt agatggagcc aggtgatccc atttcttcct gaaaacctta
     2941 gaacccgcat agcagtacga tttcacggct ctgaccttta tgaacacctg aattcagact
     3001 atatcccatg gcgaagagaa caaataaaaa gaattgattc ggttattaca gtatccgagg
     3061 caggaaagaa ttatctttca acaaaatatg ccattccaaa agagaatatt tttctttcaa
     3121 gaattggtac tcccgacttt ggattaaatc cttatgaaaa aactgaaaca gtaagaattg
     3181 tttcatgttc aaacatagta ccggttaaaa gggtggaact tatcgcactg gcattgaaat
     3241 atcttaaatt aaaggttgaa tggatacatt tcggagaggg gccattaaaa caaaaagtta
     3301 cagagcttgc catgcagctt cccggaaata tatcctggca gttcccggga gaaataaacc
     3361 atgaaaaact catgaacttc tacaaaacag tatctgttga cctttttctg aatgtcagct
     3421 cttccgaagg cgttcccatt tcggttatgg aagccctttc ctttggtatt ccagttattg
     3481 ccacaaatgc aggtggtact tctgaaatag ttactgaacc tggaggagtg ataattcctg
     3541 tagaatttga accttcattt cttgcagaaa aaattgaaaa tattgttaat gctgaaaatt
     3601 acctgcagat gagacaggca gcaagaaaaa cctgggaaga aaagtgcagg gaagacaaac
     3661 tttatccttt gcttattgaa ttccttttaa acaaccctgc tgaaaataaa aaatagctta
     3721 aaacttcaat cgtattattt aataataagt acttttgtca gttaacctta agaattaatt
     3781 tcaaagtttc cacgatgcag aactcaatta atattaaagc cactttgcca catgcagtag
     3841 caataatttt atttgctgtt gtatcgcttg tttacttcta cccagttatt gaaggcaagg
     3901 tacttgttac aaacgacggt actgtcgcat acaattctgc aagtgaaata cgtaatttca
     3961 gagaaaaata cggtcaggag cccctgtgga caaattcaat gttcggtggc atgcccgcat
     4021 atttgatttc agtaaaatat tatggaaatc ttatgcggta tgttgaccag tttctgaaat
     4081 tcatgaaact acccgtggct ccagtcttcc ttactatgac cggcttttat atacttcttc
     4141 tgttttttaa agttgattac agacttgcca ttgccggtgc cattgcctac ggattttcat
     4201 cctatttcat aattatccta ggagcaggac acaatacaaa ggcttttgca attgcatata
     4261 tggctccagt aataggaagc gtatattata gttacaaaaa agaccttata aagggatcct
     4321 tgcttctggc attttttcta accctccaga tattatcaaa tcatccccag atactgtatt
     4381 atacactgct ttgcattctg gtattcatta ttgcagagtt tatcactgct atacggacaa
     4441 aggaagttat tcctttcctg aaaaaatctc taattctgat tataccagtt ctgttgtcgg
     4501 caggaatgaa ttttgcatcg ctttatacta catacgaata cggtaaatat tcaattagag
     4561 ggaaatcgga ccttataatc cctggacaga gggaagtgaa aggtctgaac aaagattatg
     4621 caacccagtg gagttatggg attgacgaaa cacttacaat gctcattccg aactttaaag
     4681 gaggtgcaaa cagaccattc gatacaggtt ctgccactgc aaaggcatta aggctgaaca
     4741 atgcatcgca atatgctgcc aattttcaca gatactgggg ctctcaacca tggaccgatg
     4801 gccctgtata tgtgggtgca atagttgtgt ttctttttat aatgggtctt atacttatta
     4861 agggaccaat aaaatggtgg ctggccattg caactctttt gtctgttatg ctatcctggg
     4921 gcaaaaactt catgccactt acagatctat tcttttatta tttccccggt tacaataagt
     4981 tcagagctgt aactatgatt ttagttatag cagaattctg catgccgctg cttgccatat
     5041 taacacttaa agaaatattt gaaaaaagaa tttcacaaaa agatttttta aaagcattga
     5101 aaatatccct gggcatcaca ggagggctaa cattactgtt cctgttattc ccgggtcttg
     5161 cgggttcttt cctttccccc ggagagcgtg aaagtcagtt accaggatgg ctcacagagg
     5221 ctctgaaaaa agacagaatt gaac
//
LOCUS       DAOSNB010000181         5237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15890_length_5237_cov_3.471633, whole genome shotgun sequence.
ACCESSION   DAOSNB010000181 DAOSNB010000000
VERSION     DAOSNB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15890_length_5237_cov_3.471633"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLG42_07775"
     CDS             complement(<1..252)
                     /locus_tag="PLG42_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU31145.1"
                     /translation="MIKYIAIKNFKAFKSLKLKPSNLNLCFGMNGMGKSSLLQSLLLL
                     RQSFQKGLLDTKGLLLNDKDLISIGTGKDAYYQSAGRGEH"
     gene            complement(245..1381)
                     /locus_tag="PLG42_07780"
     CDS             complement(245..1381)
                     /locus_tag="PLG42_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOU31146.1"
                     /translation="MVITKNYDNAKDLVEDELLYYGPVAEISKPFDPKKIDIQTKQMI
                     LEAIFRRLRNGEINMLTGFQRKSDLWDETKQSRLIESILIRIPLPAFYFDGTNDNDWL
                     VVDGLQRLSTFKNFVINKILTLQNLEYLTQFNGFKFDNLPRDLQRRIEEHEITVYIIN
                     PGTPPEVKYNIFKRINTGGLILEPAEIRHALNQGKAADYIKKLAEIQEFTSATTNSIP
                     TERMLDRDFVTRFIAFYLHPVSEYKGNLEEYLNSAVSELKDLSEARLQTITNDFISSM
                     KAARAIFGNRAFRKIYKKSDKRKPLNKALFEVWSVLLAKLSDTERNKLITNKDFVIEQ
                     FMESLNSSDKKLDKAISTGTADKQNVINRFSIIETVIRNSLAHD"
     gene            complement(1610..2632)
                     /locus_tag="PLG42_07785"
     CDS             complement(1610..2632)
                     /locus_tag="PLG42_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31147.1"
                     /translation="MEKKISLTNTKSEILNAYNELLKRIQDSKQESPKAEQEKKVKEA
                     TVEDAAKLTDEEIIGAISSLKLSLNSKLDRIEDDLTTERQKLAKIREAILIEDQRLKD
                     LYQINAGADSLAVILAVQKEKKEEFERETTQRKSELEEEIRQEKLIRDKETKLWEENR
                     KEAEETLKKQRVREEEEFQYNLKLTRKKDKDEYEQKKFQLEKELKEKLEAFDSGIKLR
                     EQSVAAAEQELEGLRSKAESFPAELEKAVQVAVKDVTSRLEKDYKFEKQLLLKDHEGE
                     VKLKNQQIESLIAKINDLNIQLKQAYSKAENAENNSKEITLKAIQASGQIKIIEKEET
                     RRKVEE"
     gene            2954..4639
                     /locus_tag="PLG42_07790"
     CDS             2954..4639
                     /locus_tag="PLG42_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31148.1"
                     /translation="MKIKSGYIIIFIFIYLFLPVERISSQEIPHPVANTGIYQFLDEL
                     ANDQVVQLSSVVKPYSRLFIARCLRDADEKRDRLSPRQQRELDFYLRDFGKEDSSRNK
                     LFRSSFSSNFEKVSSAREDLFSRLIKVIRGNKRWDLFYYSDSLFSLTINPIIGGEFFT
                     NSSGTATYWRNGIEARAYVNRWGFYASLRDNHEKPLLGKPSFLTQREGGHLKGSSDWS
                     EMQGGITYSWKWGNAGFVKDNIEWGNNYNGANIFGGRNPSFIQLRLQLRPAKWFTFNY
                     FHGWLNSMVVDSANSYWMSNSYGTNYREVYHRKFLTANMLTVTPFKYLNISAGNSIVY
                     NNTSVNPAYLMPLFFYKSVDHSVSSGIDNMNSQMFFDISSRQIRHLHLYATLFVDELA
                     ISRISKKDAWNFLSWKSGFRLNNFPFSDTYFTTEFTYSYPLTYQHYVPTTTFESNNYN
                     LGHYLKDNSREWFISAGYRPVGNLDIQLFFSDAVRGPDYTELAAPRVGNPPLESVEWR
                     NTSAGLKAVYQLINDLYAQFSFSYSNISGNKNWSPEYLQGKKLTVNFGITFGF"
     gene            4844..>5237
                     /locus_tag="PLG42_07795"
     CDS             4844..>5237
                     /locus_tag="PLG42_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31149.1"
                     /translation="MKRNKFRVITLFTDIVILAVSFMIAVLFRPAGISSYVPSHGVFF
                     IILAVIWLTVSIVNGKMHRGKIINFTSLFNRVIGSNLISVSLLALIMYIIRDYSYSRA
                     VFLGTTVIATIFELLAGSVYIAYKKANIR"
BASE COUNT         1428 a         1109 c          937 g         1763 t
ORIGIN      
        1 atgttcacct ctacctgctg attgataata agcatcttta ccagtgccaa tagaaattag
       61 atctttgtcg ttaagtaata atcctttagt atcaagtagg cctttttgaa atgattggcg
      121 caataacaac aatgattgaa gaagtgatga ttttcccata ccattcatac caaaacataa
      181 gtttaggttt gaaggtttta atttcaggct tttaaaagcc ttgaaatttt taatagctat
      241 gtatttaatc atgggctaat gaatttctta tgactgtttc aattatcgaa aaacgattaa
      301 taacattttg tttgtcagca gtaccggtac ttattgcttt atccaatttt ttatctgaag
      361 aatttagtga ctccataaac tgttcaataa caaaatcttt gttcgttatt agtttatttc
      421 tttctgtatc ggacaattta gccaaaagta cagaccacac ttcaaaaaga gctttgttta
      481 atggcttgcg tttatcggat ttcttatata tttttctgaa agcacgatta ccaaaaatag
      541 cccttgctgc tttcattgat gaaataaaat cattggttat agtttgtaac ctggcttctg
      601 ataagtcttt caactcactt acagcgctat taaggtactc ctccaaattg cctttatatt
      661 cagaaactgg gtgtaaataa aaggctatga atcgggtaac aaaatcccgg tcaagcattc
      721 tttcggtagg tattgaattt gttgtagctg aagtgaattc ttgaatttct gcaagttttt
      781 taatataatc tgcagcctta ccctggttca aagcatgtct tatttctgct ggttccaata
      841 tcagacctcc tgtattaatt cttttaaaga tattatattt tacctctggc ggtgtacctg
      901 gatttattat ataaacagtt atctcatgtt cctcaatcct tcgttgcagg tctcttggaa
      961 gattatcaaa tttaaaacca ttgaattgtg tgagatattc cagattttgg agcgttaaaa
     1021 ttttattaat tacgaagttt ttaaatgtac ttaatcgctg gaggccgtct acaacaagcc
     1081 aatcgttatc attagtacca tcaaaataaa atgccggtaa agggattctg ataagtatcg
     1141 attcaattaa gcggctttgt tttgtttcat cccaaaggtc tgatttacgt tgaaaaccag
     1201 taagcatatt tatttcacca tttcttaaac gacggaaaat ggcttcaaga atcatttgct
     1261 ttgtttgaat atcaatcttc tttggatcaa aaggtttcga tatttcagca actggaccat
     1321 agtacagtaa ctcatcttct accagatcct tggcgttatc gtaatttttt gttattacca
     1381 tcttattccc aataatattt acaacattct tttgttaaca cacatgactg cctcggatat
     1441 catggcttcc tagtactcgc tcgctaatca attcatttta tcgctataga atctcaacct
     1501 ataaacagtt gcttttgtta tcgatttttg acggaaagag cctggtttct ccggattttt
     1561 cggtacattc cactggtcgt ggattataaa agagttatga cctcacacct tactcctcaa
     1621 ctttcctccg tgtttcttcc ttttctatta tttttatctg tcccgatgcc tgaatagctt
     1681 taagtgtgat ctccttggaa ttattctctg cattctcagc tttggaatag gcctgtttaa
     1741 gttgaatatt aaggtcattg atctttgcaa tcagcgattc gatctgttga ttctttaact
     1801 ttacctctcc ttcgtggtct ttaagaagaa gttgtttttc gaatttgtaa tccttctcaa
     1861 gccgggaagt tacgtccttt actgcaacct ggactgcctt ctcaagttca gccgggaagc
     1921 tttctgcttt tgatctcaat ccttccagtt cttgttcagc agcagccaca ctttgttccc
     1981 tgagcttaat cccgctatca aaggcttcca atttttcttt cagctctttt tcaagctgga
     2041 acttcttctg ctcatattca tccttatctt ttttcctcgt aagcttaaga ttatactgga
     2101 attcctcttc ttcacgaacc cgttgctttt tgagggtttc ctctgcttct tttctgtttt
     2161 cttcccataa cttcgtttct ttgtcgcgaa tcagtttctc ctgcctgatc tcttcttcca
     2221 gctcgctttt tctttgtgtc gtttcacgtt caaattcctc cttcttttct ttctggactg
     2281 caagaattac agcaagtgaa tcggctcccg catttatctg ataaagatcc ttcagccttt
     2341 gatcctcaat tagtattgct tctctgatct tagccagttt ctgacgttca gtggtgagat
     2401 cgtcttcaat cctgtcaagc ttgctgttca atgaaagttt caatgaactt atagctccaa
     2461 tgatctcttc atctgtcagt tttgcagcat cctccaccgt agcttctttc actttctttt
     2521 cctgttctgc tttcggactt tcctgtttcg aatcctggat tctctttaac aattcattat
     2581 aagcattcag tatttctgat tttgtgttcg ttaaacttat tttcttttcc atggtttagg
     2641 gtttttaaat ttggtaactt ctttttattc attcttttaa ataatcatgg cccggaattt
     2701 cagaaaaata tgactgtaat attacaaaga taaatatatt caaaccttcc acaaaaaaac
     2761 cagatacata tatagttttg atattctgat atttaacaag ctatccggaa cagatctgcc
     2821 gctaccggga actaaaaaca tcattatctt tttacccgct gatgtcatgt tttccttatc
     2881 agacaaatgc agaccgcagg aatgaaggat ttcctgcaat aaatcctata tttgtttttc
     2941 aatacgattc ctgatgaaga tcaaatccgg atatatcatc attttcattt ttatttacct
     3001 ttttctgcct gttgagcgga tatcatccca ggaaattccc catccggttg ccaatactgg
     3061 catttatcag ttccttgatg aactggcaaa cgaccaggta gtccaactca gctccgttgt
     3121 aaaaccatac tccaggctgt tcatagcccg atgcctgcgg gatgcagacg aaaaacgtga
     3181 ccggctttcc cccaggcagc aaagagaact tgatttttac ctgagggatt tcgggaagga
     3241 agacagttca aggaataagc ttttcagatc atctttcagc agtaacttcg aaaaagtgag
     3301 ttctgcaagg gaagatcttt tttcccgtct gataaaagta atacgaggca ataaaagatg
     3361 ggatcttttt tattattctg attctctctt ctcacttaca ataaatccta ttattggcgg
     3421 agaatttttt accaactcct ccggaacggc aacatactgg agaaacggaa tcgaggcaag
     3481 ggcatacgtc aaccggtggg ggttttatgc ttccctcagg gacaatcatg aaaaacctct
     3541 cctggggaag ccttcattcc tgacacagag agaaggcgga caccttaagg gaagcagcga
     3601 ctggagcgaa atgcagggcg gaatcactta ttcatggaaa tggggaaatg caggatttgt
     3661 gaaagacaac attgaatggg gaaataatta taacggtgcg aatatcttcg gcggaaggaa
     3721 tccttcattc atacagctga ggcttcagct cagacctgca aaatggttca cgttcaacta
     3781 tttccacgga tggctcaatt caatggtggt ggacagcgca aactcctact ggatgtcaaa
     3841 cagttacggc acaaattacc gggaagtgta tcacaggaaa tttcttacag caaacatgtt
     3901 aactgtcact ccgtttaaat acctgaacat ctccgcggga aacagcatag tatacaacaa
     3961 cacatcggta aacccggcat accttatgcc gctgttcttc tacaagtctg tggaccattc
     4021 agtatcatca ggtattgaca acatgaattc ccagatgttt tttgatataa gttccaggca
     4081 gatcagacat cttcacctgt acgcgactct ctttgttgat gaacttgcaa taagccgtat
     4141 atccaaaaag gatgcatgga acttcctgag ctggaaaagc ggattccggc tgaataattt
     4201 tcctttcagc gatacatatt tcactacgga attcacttat tcctacccgc ttacctatca
     4261 gcattatgtg ccgaccacta catttgaatc aaacaattac aacctcgggc actatcttaa
     4321 ggataattca agggaatggt tcatatctgc cggataccgg ccggtgggca atctggatat
     4381 tcagctgttt ttctcagatg cagtcagagg accggattat accgaacttg ctgcccccag
     4441 ggttggcaac cctccccttg aatcagttga atggaggaac acatctgccg gactgaaagc
     4501 tgtttatcag ctgatcaacg atctatatgc gcaattttcg ttttcttaca gtaatatttc
     4561 cgggaacaag aactggagtc cggagtattt acagggaaag aaattaacag ttaattttgg
     4621 aatcacattc ggattctagc aaggccggat atacatatat atcagaaatc ctgtgtgtta
     4681 atccgtatct atttaaaact ggtctgataa aaaatctgcc ggaaaacatc acagcaacag
     4741 ctgttcttca agaccgttga aaaattccct gaatccccgc tcgtttttta gctgaaaaaa
     4801 cttatatttg gcacgtcctt tatcagggac ctactgtcaa attgtgaaaa gaaataaatt
     4861 cagggtaatc acgctgttta ccgatattgt tattcttgca gtatcgttta tgatcgcggt
     4921 ccttttcagg cccgccggga tcagttccta tgtcccttcc catggtgttt ttttcataat
     4981 cctggccgtg atctggctga ctgtctccat agtcaacggc aaaatgcata ggggtaagat
     5041 tattaatttt accagcctct tcaacagggt aatagggagc aacctgatat ccgtgtccct
     5101 tctcgccctg ataatgtata tcataaggga ttattcctat tcacgtgccg tgttcctggg
     5161 aaccacagta atagcaacca tcttcgaact tttggcggga tctgtgtata tagcctataa
     5221 aaaggccaat atccgcg
//
LOCUS       DAOSNB010000182         5216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_15994_length_5216_cov_4.837241, whole genome shotgun sequence.
ACCESSION   DAOSNB010000182 DAOSNB010000000
VERSION     DAOSNB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15994_length_5216_cov_4.837241"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLG42_07800"
     CDS             complement(<1..1060)
                     /locus_tag="PLG42_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU31150.1"
                     /translation="MKYNNPRRSIYLPIIIAVSVSLGIIAGLLLPGNKSGILSTSIRP
                     KYDKIPTLLNIINSSYVDTVNIPELEESAISTVLKNLDPHSIYIPAKDMKRANEPLRG
                     NFEGIGITFNLLTDTILVISTIPGGPSEKAGLMAGDKIIYVNDSLVAGVGITEDGAMG
                     MLRGPRGTVVKVKVLRKGSAGLIPFEITRDKIPLHTVDVAYMINNSTGYIKINNFAVN
                     THNEFTEALKDLKNQGLRSLILDLRGNAGGVMDPAIKIANQFLKKGQLIVYTQGRAFP
                     RSEARATGNGLFEEGELVVLIDEYTASASEILAGAIQDNDRGTIIGRRSFGKGLVQEP
                     ITFSDGSGLRLTVARYYTP"
     gene            complement(1047..1517)
                     /locus_tag="PLG42_07805"
     CDS             complement(1047..1517)
                     /locus_tag="PLG42_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOU31151.1"
                     /translation="MKKPDILKPYGDKQPVFDKRYLEMALIWAKNSYCKRRQVGALIV
                     KDKMIISDGYNGTPAGFENVCEDENGLTKPYVLHAEANAITKVAKSNNSSENATLYVT
                     SSPCMECSKLIIQSGIKRVVYCDIYHNTDGLHLLKRAGIEIVYVDLEKDIYEIQ"
     gene            1770..2351
                     /locus_tag="PLG42_07810"
     CDS             1770..2351
                     /locus_tag="PLG42_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOU31152.1"
                     /translation="MKKFFVLILLSAVYISVNAQGTFKDPRDGNVYKTVTIQGMVWMA
                     ENLRYKAPGGAYYFDNNPNTIQQYGMLYDWKTAMKACPDGWRLPTGDEFRALITNNEV
                     KEAMKARPNESNAYSIQLAGMQDHEGNLTEIDESGYYWTSTEYDENNAEYFSYLTVFN
                     APVADISRKEDIGDMHGTEKTNKYSVRCVKIQK"
     gene            2414..3133
                     /locus_tag="PLG42_07815"
     CDS             2414..3133
                     /locus_tag="PLG42_07815"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HOU31153.1"
                     /translation="MASSLADRIIAFYRNLDIRETLPPGISIMNPYRKDSRVMKVVEE
                     FYRKFYSDNNPRNIILGINPGRFGAGITGITFTDTIRLWEKCGIIMEGTESRELSSEF
                     VYEMIDRFGGVNRFFSHFYLSAVSPLGFTLTAKEGKEINFNYYDNPDLLRAVYDFIVE
                     NIERQLDFGIKNDVCFCLGTGKNYRFLKEINEKYGFFNKIIPLEHPRFIMQYRRKVKD
                     FYITRYIGAFSETINKKRFPF"
     gene            3222..3563
                     /locus_tag="PLG42_07820"
     CDS             3222..3563
                     /locus_tag="PLG42_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU31154.1"
                     /translation="MASPKKERYSNEDILIARFAKALSHPARVSILKHLASLDTCCFN
                     EISKVIPLADSTVSQHLTELQKAGLIQGSIEAPRIKYCINWKNWQLLRKVLSDFADMK
                     ISKYEKEFSEL"
     gene            3560..3976
                     /locus_tag="PLG42_07825"
     CDS             3560..3976
                     /locus_tag="PLG42_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOU31155.1"
                     /translation="MIKNDSLKGFFAHNVLNYTPAECYRMCKEGATIVDVREGYMTSF
                     KMFDVDNIIYIPFSRFTELYNSLPCDVTLIFADSAGLKSRECVLYMVKNGYTNVANMA
                     GGMVEWERDGLPVKVDKNFRLSGSCMCQIKAREKGK"
     gene            3997..>5216
                     /locus_tag="PLG42_07830"
     CDS             3997..>5216
                     /locus_tag="PLG42_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOU31156.1"
                     /translation="MIRHRIVSLLPYLAVLLLAFFITGLIFSKAQNTVNKKERTTQRS
                     VCPPFNLYDEDGNLIDPVHGINADKPYSPKQTCGKCHDYDKITQGFHFQQGRGEELPE
                     WMAERYQWVSFPGNYGGTWCSPAPLYRHLSPKKNSTARTIDMTSFSFITAGCGECHPG
                     GGSAEYDREGNRYDRFMVEKGYKPGAENDLDGDYFQARWSETGVLEADCMICHLPGYN
                     NDERKNQLKLLNFRWAPVAGSGLGTVTGSVDKNEPVKVTYDISKFDAEGKLSPHIVRE
                     PRNEACLFCHAQPGWKKRGANYDERTDVHLRAGLKCVDCHPAGSRAKDSRINEREMHQ
                     FGKGDDPGGHVRDDLDNTVLDCNYCHTNGNMGAPVAKHTWLPQLHLDHLACQTCHVPE
                     RIVKAAQVQASDVF"
BASE COUNT         1556 a          937 c         1160 g         1563 t
ORIGIN      
        1 tgggtgtgta gtatcttgca actgtaagcc gcaggcctga gccgtcagaa aaagttatag
       61 gttcctgcac cagtcctttg ccgaaagagc ggcgtccgat aatagttcct ctgtcgttat
      121 cctgtattgc accggcgaga atttcactgg cagaggcagt atattcatct atcagtacaa
      181 ccagttctcc ttcttcgaag agtccattac cggtagctct ggcttcgctt ctagggaatg
      241 ctcgtccctg tgtataaact atcagctgtc cctttttaag aaactggttg gctattttta
      301 ttgccgggtc cattacacct ccggcattac ccctgaggtc cagaatcaga cttcttaagc
      361 cctggttttt aagatcttta agggcctcgg taaattcatt gtgtgtattt acagcaaagt
      421 tgttgatttt gatgtagcct gtgctattat ttatcatgta ggctacatca acagtatgca
      481 atggaatttt gtcgcgtgtg atttcaaatg gaatcagtcc ggcggagcct ttgcgtaaaa
      541 ctttgacttt taccactgta ccccttgggc ccctgagcat tcccatggct ccatcttctg
      601 ttattccaac gcctgctaca agggaatcat tcacatatat tattttgtca cctgccatca
      661 gtccagcttt ttcagacggt ccaccaggaa tggtacttat tacaagaata gtgtcggtga
      721 gcaggttgaa tgtaatgcct atgccttcaa agttgcctcg aagcggttca tttgcacgct
      781 tcatgtcttt tgcagggatg taaattgaat gaggatcaag attcttgagt acagtactga
      841 tagcagattc ctccagctct ggaatattta ccgtatcgac atagcttgaa ttgattatgt
      901 tgagtaatgt tggaattttg tcatacttcg gacggatact ggtggagaga attccgcttt
      961 tattcccggg aaggagcaac ccggcaataa ttcccagtga aacagaaaca gcaattataa
     1021 tgggaagata tatgctgcgt cttggattat tgtatttcat aaatatcttt ttccagatca
     1081 acgtaaacaa tttctatacc tgctctttta agcaggtgga gtccatcggt attatgatag
     1141 atgtcgcagt aaacgactct ttttatcccc gactgaataa ttagctttga gcactccatg
     1201 cagggtgagc tggttacata aagggttgca ttttcgcttg aattatttga ttttgcaacc
     1261 ttcgttattg catttgcttc ggcatggaga acataaggtt ttgtcagtcc gttttcatct
     1321 tcacacacat tttcaaaacc agccggtgtg ccattatagc cgtcggaaat aatcattttg
     1381 tctttaacta tcagcgcccc tacctggcgt cttttgcagt atgagttttt tgcccagata
     1441 agagccattt cgagataacg tttgtcgaaa actggctgct tgtcgccata aggtttgaga
     1501 atatctggct ttttcatccg gaaaaacagg ttttcaaaaa cgtacaaaat taagaatttt
     1561 tctgcaggtt aggattttat gatgaatgat tgccggagat gtatataaac tgttataagg
     1621 cagggtgtta attttttttt gctggcattt gataatattt tgaaatttta aatgtttatt
     1681 attcatgtta acagtatatt tgcatttcct taagccgtta aagatggtat gaattttgag
     1741 ggaaaaatga gttaaaaata attttctata tgaaaaaatt ttttgtttta atattattat
     1801 cagccgtgta tatttccgtc aatgcccagg gtacttttaa ggatccccgc gatgggaatg
     1861 tttacaaaac tgttaccata cagggaatgg tgtggatggc tgaaaacctg aggtataaag
     1921 ctcccggagg ggcctattat tttgataata accctaatac gatacagcaa tacgggatgc
     1981 tttatgactg gaaaacagct atgaaggcct gccctgatgg ctggaggctt ccaacgggtg
     2041 atgaattcag agcacttatt acaaataatg aagttaagga ggcaatgaaa gcacgtccta
     2101 atgaatcaaa tgcttacagc attcagcttg caggtatgca ggatcatgaa ggtaatctta
     2161 cagaaataga cgaaagcggg tattattgga catcaactga atatgacgaa aacaatgctg
     2221 aatatttcag ttatcttacg gtattcaatg ctcccgtggc cgatatatcg aggaaagaag
     2281 atatcggaga tatgcacggc acggaaaaga caaacaagta ttcagtacgc tgtgtgaaga
     2341 ttcaaaaata aatggcatta atcatgtttt taagtcagga ttcatatatt tatggatcct
     2401 gattttttat tttatggcat catcactggc agacaggata atagcatttt acaggaatct
     2461 tgatattaga gaaactcttc cacctggtat atcaataatg aacccttaca ggaaggattc
     2521 ccgggttatg aaagtggtgg aagagtttta caggaagttt tacagtgata ataatccgag
     2581 aaatattatt cttggtatca atccaggaag gttcggtgcc ggaataaccg gaattacatt
     2641 tactgatacg ataaggctat gggaaaagtg tggaattatt atggagggga ctgagtcaag
     2701 ggaactctca tctgagtttg tgtatgaaat gattgataga tttggcggtg taaatagatt
     2761 tttttcccat ttctatttga gtgctgtgag tccgctcggg tttaccttga ccgctaaaga
     2821 aggtaaggaa attaatttca actactatga taaccctgat cttttaaggg cggtttatga
     2881 ttttattgta gaaaatattg aacgtcagct tgattttggt ataaaaaatg atgtgtgttt
     2941 ttgtctgggc accggcaaaa attacaggtt tcttaaagaa atcaatgaaa aatatggttt
     3001 ttttaataag attattcccc ttgaacatcc gaggtttatt atgcaataca gaaggaaagt
     3061 taaagacttc tatataacca gatacattgg tgcattttca gagacaataa ataaaaaaag
     3121 gtttccattt taacattttt tacgaataaa aagtttgtgt tgttgatttc attttcgtat
     3181 ttttacgaaa tacaaagtat aatcaactat tttttaagca gatggcatca ccaaagaagg
     3241 aaaggtacag caatgaagat attcttattg caagattcgc aaaagcactt tctcacccgg
     3301 cgagagtctc tattctgaaa catcttgcat cgcttgacac ttgttgcttt aatgaaatat
     3361 caaaagtgat accattggct gattcaactg tttcacaaca tctgacagaa cttcagaaag
     3421 caggtcttat acagggaagc attgaggcgc ccagaattaa atactgtatt aactggaaaa
     3481 actggcaatt gcttcgtaag gtgttaagtg actttgcaga tatgaagata tcaaaatatg
     3541 aaaaagaatt ttcggaatta tgattaaaaa tgactcgtta aaaggctttt ttgcccataa
     3601 tgttttgaac tacacccctg ctgaatgtta cagaatgtgt aaagagggtg caacaattgt
     3661 ggacgtaaga gaaggttaca tgacaagttt taaaatgttt gatgtggata acattattta
     3721 cataccattc agcagattta cagagttata taacagtttg ccatgtgatg tgacattgat
     3781 atttgctgac tctgcgggac tcaaaagcag ggaatgtgta ctgtatatgg ttaaaaacgg
     3841 atataccaat gttgcaaata tggcaggcgg aatggttgaa tgggaaaggg atgggttacc
     3901 tgttaaggtt gataaaaatt tcaggctttc gggttcatgt atgtgtcaga ttaaagccag
     3961 ggaaaaaggt aaatagcaaa ataaatctta aaaactatga tccgacatag gattgtttct
     4021 cttttgccat atttagcagt tcttctactg gcatttttta ttactggttt aatattcagc
     4081 aaagctcaaa acactgtcaa taagaaagaa aggacaaccc agagaagtgt ctgtccacct
     4141 tttaatctgt atgatgagga tggcaacctt attgatccag ttcacgggat aaatgctgac
     4201 aagccttatt cgcccaaaca aacatgcggc aaatgtcatg attatgacaa aattacgcag
     4261 ggattccact tccagcaggg taggggtgaa gagttgcctg aatggatggc agagcgttat
     4321 cagtgggttt cctttcccgg gaactatggt ggcacatggt gttcaccggc acctctgtac
     4381 aggcatcttt caccaaaaaa gaattctaca gcccgtacga tagacatgac ttcattcagc
     4441 tttattaccg caggatgcgg ggaatgtcac ccgggtggcg gttcggctga atacgacagg
     4501 gaaggcaacc ggtacgacag gtttatggtt gaaaaaggat acaaacctgg cgcagagaat
     4561 gatctcgacg gagattattt tcaggctcgc tggagtgaga caggggttct tgaggccgac
     4621 tgtatgatat gccacctgcc gggatataac aacgatgaaa gaaaaaacca attgaaactt
     4681 cttaacttca ggtgggctcc ggttgcaggc tctggacttg gcacagtgac gggttcagtt
     4741 gataaaaatg agcctgtgaa agtaacatat gatatttcaa agtttgatgc tgaagggaaa
     4801 ttgtcaccgc atattgtaag ggaaccaaga aatgaagcat gtcttttttg ccatgcacag
     4861 ccgggatgga aaaaaagggg agctaactat gatgaaagaa cagatgttca tctgagagcc
     4921 ggccttaaat gcgtcgactg tcatcccgcc ggctcaaggg caaaagattc ccggataaat
     4981 gaaagggaaa tgcaccagtt tggcaaggga gatgatcccg gcggacatgt aagggatgat
     5041 cttgacaaca ctgtacttga ctgtaattat tgtcacacca acggcaatat gggggctccg
     5101 gttgccaaac acacctggct accccagctg cacctcgacc atctggcctg ccagacatgc
     5161 catgtacccg aaagaatcgt taaggccgcc caggtgcagg caagcgatgt atttaa
//
LOCUS       DAOSNB010000183         5204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16042_length_5204_cov_3.617401, whole genome shotgun sequence.
ACCESSION   DAOSNB010000183 DAOSNB010000000
VERSION     DAOSNB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16042_length_5204_cov_3.617401"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PLG42_07835"
     CDS             complement(<1..759)
                     /locus_tag="PLG42_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOU31157.1"
                     /translation="MTERAEFYEEKTTEEITGAEALLRCLIEEGVDTIFGYPGGAIMP
                     VYDKLLDYKEKIRHILTRHEQGAAHAAQAYAMVTGKPGVAFATSGPGATNLITGIANA
                     YLDSVPVVFITAQVVSSLIGTDAFQETDIIGISMPVTKWNCQIKRAEEIPEMVAKAFY
                     IATTGRPGPVLIDITKDAQTGKLPFKYKKCRFIRSYNPRKPLKKKEIESAAILINHAE
                     KPLILAGHGVLISKAENELREFAEKTGIPVAVTLL"
     gene            complement(817..2646)
                     /gene="ilvD"
                     /locus_tag="PLG42_07840"
     CDS             complement(817..2646)
                     /gene="ilvD"
                     /locus_tag="PLG42_07840"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOU31158.1"
                     /translation="MKKNLRSSVTINGRTMAGARSLWRANGMKDEHFGKPVFAIVNSF
                     TQFVPGHVHLHNVGQFIKEHIEKRGFFAAEFNTIAIDDGIAMGHNGMLYSLPSRELIA
                     DSIEYMMNAHMADAMICITNCDKIIPGMLMAAMRLNVPFVIVSGGPMEAGTFGDTHLD
                     LVDAMVAAADDRVSDADLLMIEKSACPTCGSCSGMFTANSMNCLSEAIGLALPGNGTV
                     LATHKNRRILFERAADTIVNITLRYYYDGDESVLPRSIATREAFLNAMALDIAMGGST
                     NTVLHLLAIAYEAGVNITMKDIDELSGKVPNICKVAPSSKYHLQDVNRAGGILSIMGE
                     LWRNNLIDSTVKRIDYPSLGDAISEWDIMSDRVIPLALDFWRSAPSGEKRPGEGFFSS
                     AIYRELDKDRANGCIRDFEHAYSRDGGLAVLYGNIAKNGCVVKTAGVDPALLTFRGPA
                     RIFESQEDAVDGILGGKVAAGDVVVIRYEGPKGGSGMQEMLYPTSYLKSKGLAKKCAL
                     ITDGRFSGGTAGLSIGHISPEAAAGGEIALLREGDIIEINIPERSINVQLDDEEIMKR
                     RKEEEQKGDAAWKPASRDRKIPASLRAYAAHVTSADKGAVRMI"
     gene            complement(2920..4785)
                     /locus_tag="PLG42_07845"
     CDS             complement(2920..4785)
                     /locus_tag="PLG42_07845"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOU31159.1"
                     /translation="MLLLTASVPAVREVSVNSPDNSIRFNLSVKNGSPLFSATLNNSP
                     LITPSPIVMSVDGIKVTDGIKIASVKRFSETDTYPVYGLHSTAINRCNGARIALTHTN
                     SGLKYYMDIKVFDSGFGFRFVVPGKDDEERIPGEATEFNVPSGSQVWYHNLRMHYEGE
                     YSRKLIDTVPAGEWAAPPVTFKLPDGRGYGSITEANLSGYAGMALQTSRNKGFTIRLG
                     HEHPVSYPYELRYTREDIERVSKSASVRGTITTPWRVVLAGKELNTLVNSDVITSLCP
                     PPDKKYFPEGLFTPWIRPGRAVWRYLDGGGDNSLRNMKEFSRLAGEMGFEYNILEGFW
                     SRWPDDSVKALVDYSRNMGVGIIVWKHSKELRDPEERQQFLSRLVNLGIAGIKVDFFD
                     HEAKEVIDFYESLFRECAALKLVLILHGANKPTGLQRTWPNVMIYEAVRGMEASRLVD
                     RATHETTLPFTRMLAGPADYSVCHFGERRRNTTWVHQVATAAIYAAPVITYAANPFNL
                     VSNPCAEMIKSIPPVWDQTIVLAPSEIGEIAVYAQRKGSTWFLSVINGAEPKTVKIPL
                     SFLENITYNTLCLKDSQKDTAQVIIEKSKASKNDIISVNLGTGGGYMCRFSPQQP"
     gene            complement(5008..>5204)
                     /locus_tag="PLG42_07850"
     CDS             complement(5008..>5204)
                     /locus_tag="PLG42_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOU31160.1"
                     /translation="SKNDFVHKMKVCLKELRESHNCLKIINKGIISEKDELVARLITE
                     SNELISIFVKSINTALKKSS"
BASE COUNT         1374 a         1185 c         1066 g         1579 t
ORIGIN      
        1 aagaagggtt acggcaacgg gaatacctgt tttttcggca aattccctga gctcattttc
       61 tgcttttgat attaataccc cgtggcctgc aagaatcagg ggcttttcgg catgattgat
      121 taaaattgcg gcagattcta tttctttctt tttcaggggt ttacgaggat tgtaacttct
      181 tatgaatctg cactttttgt atttgaatgg cagtttgcct gtctgtgcat cttttgtaat
      241 gtcaataagc acaggtcccg gccttcccgt ggttgcaata taaaaagctt ttgccaccat
      301 ttccggaatc tcttctgccc gttttatctg gcagttccat tttgttacag gcatcgagat
      361 tcctattatg tcggtttcct ggaaagcgtc ggtacctata aggctggaaa ccacctgggc
      421 tgtaataaat actacgggta ccgaatcgag gtaggcattg gcaatgcctg ttatcaggtt
      481 tgttgctcct gggcctgatg tggcaaaggc tacaccgggc tttcctgtaa ccattgcata
      541 tgcctgtgcg gcatgagcag caccctgttc atgacgggtt agaatatgtc tgattttttc
      601 tttgtaatcc agaagtttat catacacagg cataattgct cctccgggat agccgaaaat
      661 cgtatcaaca ccttcttcaa tcaaacaccg gagcagcgct tctgcacctg taatctcttc
      721 agtggttttt tcttcgtaaa attcagctct ttcagtcatg ctttctgctt tgtcgtaatt
      781 aaacataata ttgacaatta aaaaattagt tctgaatcat atcattctta cggctccttt
      841 atctgccgat gtgacgtgtg ccgcataggc tctcagcgat gccggtattt ttctgtctct
      901 cgatgcaggt ttccaggctg catcaccctt ctgttcctct tccttcctcc gtttcataat
      961 ttcctcatca tcaagctgga catttataga tctttccggt atatttattt caattatatc
     1021 tccctcgcgc agcaatgcta tttcaccacc tgcagcagct tcaggcgaga tatgtccgat
     1081 tgataatcct gccgttcctc ccgaaaacct cccgtcggta atgagtgcac atttttttgc
     1141 cagccctttt gatttcagat atgaggtcgg ataaagcatc tcctgcattc ccgatccgcc
     1201 tttaggccct tcatagcgga ttacaacaac atcaccggca gcaactttcc ctccgaggat
     1261 gccatctact gcatcttcct gcgactcgaa tattcttgcc gggcctctga aagtgagaag
     1321 ggcagggtcg acgcctgctg tttttaccac gcaaccattt tttgcaatat taccatacaa
     1381 taccgcaagc cctccgtcgc ggctgtaagc gtgctcaaaa tctcttatgc agccattggc
     1441 tctgtcttta tccagctctc tgtatattgc cgaactgaaa aatccttcac cgggcctttt
     1501 ttctcccgag ggagcacttc tccagaaatc gagtgcaaga ggaataaccc tgtcgctcat
     1561 gatgtcccat tccgaaatgg catcgccaag ggaaggataa tcaattctct ttacagtact
     1621 gtcaataaga ttgtttctcc agagttcacc cataattgag agtatccccc cagcacggtt
     1681 aacatcctga aggtggtatt tcgagctcgg ggcaactttg cagatattgg gcaccttacc
     1741 cgatagttca tcaatatctt tcatggtaat gtttactcct gcttcatagg ctattgccag
     1801 aagatgaagg acggtgtttg ttgaaccacc catggctatg tcgagtgcca tggcattcag
     1861 aaaggcttca cgtgtagcga tggagcgggg gaggacagat tcatcaccgt cgtaataata
     1921 tctgagagtt atgtttacaa tagtatcggc agctctctca aaaagaatcc gccggttttt
     1981 gtgtgttgcc agcaccgttc cgttacccgg cagagccaga ccgatagctt ctgacaggca
     2041 gttcattgag ttggcggtga acattcccga gcacgacccg caggtagggc aggccgattt
     2101 ttcaatcatc agaaggtcgg catcacttac tctgtcgtcg gctgcagcca ccatggcatc
     2161 aacaaggtca aggtgcgtat caccaaatgt gccggcttcc attggtcccc ccgatacaat
     2221 tacaaaaggt acattcaatc gcatggcagc cataagcatg cccggtataa tcttgtcaca
     2281 gtttgtaatg caaatcatgg catcggccat atgtgcattc atcatatact ctatgctgtc
     2341 ggctataagt tccctcgacg gcagggaata aagcattccg ttatgtccca tggcaattcc
     2401 gtcgtcaata gcaatcgtgt taaattctgc agcaaaaaat ccacgttttt caatgtgctc
     2461 cttaataaat tgtccaacat tgtgaagatg aacatgaccc gggacgaatt gcgtgaaaga
     2521 attcacaatg gcaaaaacag gctttccaaa gtgctcatct ttcatcccgt tggcacgcca
     2581 caggcttctc gcaccggcca ttgtccggcc gttaattgtg actgaacttc ttagattctt
     2641 tttcataaaa atcttcttaa aaaaagagct ttccgggatt gacccggaaa gctctgtaaa
     2701 tttgtatatt acatactatt tccgggtcat cttcggaaaa gaagaagaat aataattatg
     2761 gaaatagtaa gtaataaaaa tctattcatt tttctcaaaa ttgattgaca aaggtattaa
     2821 ttaattttaa aattcaaaca aaactttgaa attttattaa aaaaatttat taactcctcc
     2881 atcataatat ataatataag gtataagctc tggttcctgt cagggttgtt gcggtgaaaa
     2941 cctgcacata tacccgccac ctgttccgag attaactgat atgatatcat tcttcgatgc
     3001 cttacttttt tcgattataa cctgagcagt atctttctga gaatctttca gacacagggt
     3061 attataagtg atattttcaa gaaatgagag gggaattttc acggtttttg gttctgcacc
     3121 attaataacc gatagaaacc atgtacttcc ttttctctgt gcataaactg caatctcacc
     3181 aatttctgaa ggtgccagta caattgtctg atcccatacc ggaggaatac ttttaatcat
     3241 ttctgcacac gggttcgaaa cgaggttgaa cgggttggca gcgtatgtaa taacgggtgc
     3301 ggcatagatg gctgcagtgg caacctgatg aacccacgtg gtgtttctcc ttctttcacc
     3361 aaaatggcat actgaataat ctgcaggtcc agctagcatt cgtgtaaaag gtaaagtggt
     3421 ttcgtgggtt gccctgtcaa ccagacgact ggcttccata cccctaactg cttcgtaaat
     3481 catcacattt ggccatgtac gttgcaaccc tgttggtttg tttgctccat ggagtatcag
     3541 aacaagttta agagcggcac attccctgaa gagtgattcg taaaagtcta tcacctcttt
     3601 tgcctcatga tcgaaaaaat caacttttat gcctgcaata cccaggttga ccagtctgct
     3661 taggaactgc tgtctttctt ctggatcgcg caactctttt gaatgtttcc atacgattat
     3721 gcctactccc atattacgtg aataatcaac aagagctttt actgagtcgt ctggccacct
     3781 gctccagaac ccttcgagaa tattgtattc gaaacccatc tcaccagcaa gtcttgagaa
     3841 ttctttcatg tttctcagcg agttgtcgcc acccccgtcc agataacgcc atacagccct
     3901 tccaggtctg atccagggtg tgaacaagcc ttccgggaaa tattttttat caggtggtgg
     3961 acaaaggctg gtgatgacat ccgaattgac aagtgtgttt aattctttcc ctgcaagaac
     4021 aaccctccat ggtgtggtta ttgtgcctct cacagaggca gatttcgata ccctttcaat
     4081 atcttccctg gtgtacctga gttcataggg gtaagataca ggatgctcat gccccagtct
     4141 tattgtgaat cctttgtttc tggatgtctg taacgccatt ccggcgtagc cgctgaggtt
     4201 tgcttcggtt attgagccat agcctctgcc atcgggaagt ttgaaggtaa ccggaggagc
     4261 agcccactca ccggcaggaa cggtatcgat gagttttcgc gagtactctc cttcgtaatg
     4321 cattcgaaga ttatggtacc agacctgact ccctgatgga acattgaatt ccgtagcttc
     4381 gccgggtatg cgttcctcat catcttttcc cggaacaaca aacctgaatc caaagccact
     4441 gtcaaatact tttatatcca tataatactt cagacccgaa tttgtatgtg tcagggcaat
     4501 ccttgccccg ttgcacctgt ttatagcagt tgaatgaagg ccataaacag gataggtgtc
     4561 agtttcgcta aatcgcttca cggaagctat ctttatgcca tcagtcactt ttatcccgtc
     4621 aacagacatg acaattggag aaggagttat cagcggcgag ttgtttaaag tcgctgaaaa
     4681 gagaggcgag ccatttttca ccgataagtt aaaccttata ctattgtcag ggctgttaac
     4741 actaacttcc cggactgccg gaacagatgc agtcagaaga agcaataaaa ttgatttggg
     4801 ttgaattaag aacatgggaa cagttaaata gttgaacagt tgaattaaga acatttgaac
     4861 agttgaatta agaacattag aacatttgaa catttgaatt aagaacatta gaacatttga
     4921 acatttgaac attagaacat ttgaacattt gaattaagaa cattagaaca gtcgaacatt
     4981 ttaacagttg aattaacaat caggaagtta tgaagatttt ttaagggcag tgtttatgct
     5041 ttttacaaag atagagatta attcgttgct ttctgtaatt aagcgtgcaa cgagttcatc
     5101 tttttcagaa ataatgcctt tattaatgat tttaaggcag ttatgagact ctctcagctc
     5161 tttgagacaa actttcattt tatgaacgaa gtcattcttt gatt
//
LOCUS       DAOSNB010000184         5185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16134_length_5185_cov_4.231189, whole genome shotgun sequence.
ACCESSION   DAOSNB010000184 DAOSNB010000000
VERSION     DAOSNB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16134_length_5185_cov_4.231189"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLG42_07855"
     CDS             <1..215
                     /locus_tag="PLG42_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31161.1"
                     /translation="YKNPIFEGKRIVHGHRPKQLEYTKKLVEENSHVLPIDTGCVYGR
                     EMNYGYLTALDVNSMKLISIENIDWE"
     gene            356..1999
                     /locus_tag="PLG42_07860"
     CDS             356..1999
                     /locus_tag="PLG42_07860"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOU31162.1"
                     /translation="MKRLVFIVFSVIFTESFLFPQQPVFTRQDTLRGSITPEREWWDL
                     VYYHLNINVNPADSTISGTNTVVYRVLKSHTVMQIDLQPPLRLISATQDGRRLKIEQE
                     GNAHFIHLEKKQEAGQINSIVLEYGGRPRIAPNPPWQGGITWKYDRRGNPFIASSCQG
                     DGASLWWPCKDHMYDEPDSMLISVTVPANLVDVSNGRLRSVTENKKNRTKTWNWFVSN
                     PINNYGVNINIGDYVHFSEKFNGEKGVLDCDYWVLRDNLEKAKEHFKQVQLMLKAFEY
                     WFGPYPFYEDGYKLVEAPYLGMEHQSSVTYGNGYQNGYLGRDMSGTGWGDSWDFIIVH
                     ESGHEWFANSITYKDIADMWVHESFINYSESLFVEYYHGKEAGAEYVRGTRRSIRNDR
                     PIVGIYNVNYEGSGDMYSKGGNMLHTLRQVIGDDDKWREILRGLNSEFWHQTVDGSQI
                     EEYISKKAGMNLKPFFDQYLRDVRIPVFEYYVKDKSLTFRWNNCVQGFNMPLKIYVNK
                     KPLIINPSARWTTITLEEPASEIKVDRDYYVAVLDMMGN"
     gene            complement(2016..2498)
                     /locus_tag="PLG42_07865"
     CDS             complement(2016..2498)
                     /locus_tag="PLG42_07865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU31163.1"
                     /translation="MNLTFRTIPEKDDVKRIEEIVSSTGFFHDFETEVAVELIKERLQ
                     KGEESGYYFVFAESEGETVGYTCYGPDPVTKSTFLLFWIVTHKNFMGKGIGSRLLEET
                     HSQVKKMGGTMIVAETSGRELYAPTRAFYSKSGYIPEAIIKDYYDRGDDKYIYVKRLA
                     "
     gene            2677..4344
                     /gene="asnB"
                     /locus_tag="PLG42_07870"
     CDS             2677..4344
                     /gene="asnB"
                     /locus_tag="PLG42_07870"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HOU31164.1"
                     /translation="MCGIIGVFDLKQSPEELRPQVLTMAKKIRHRGPDWSGIYSSDRV
                     ILAHERLAIVDPISGAQPIKSSDGKLVLAINGEIYNHNELRKNLKQDYNFLTRSDCEV
                     ILALYREKGVNFLNELNGIFAFALYDSEKDVYFIARDHIGIVPLYKGWDRSGNFYVAS
                     ELKALEGVCNKIEIFQPGHYYLSSEGVEKRWYNPEWKSYEAVKNNTSDLHRLRKALED
                     AVHRQLMSDVPYGVLLSGGLDSSVISALARKFAAMRVETQDLQEAWWPRLHSFSVGLK
                     GSPDLDAARKVAEHIGTVHHEVIFTIQEGLDAISDVIYHIETYDVTTVRASTPMYLLA
                     RAIKSMGVKMVLTGEGADEVFGGYLYFHKAPDARAFHEETVRKIFKLHLYDCLRANKS
                     LAAWGVEGRVPFLDREFLDVAMTLNPEDKMVKNGRLEKWILRKTFEDILPESVVWRQK
                     EQFSDGVGYNWIDTLKEIANRKVTDAQMRTAAERFPVNPPLTKEEYLYRSIFSQHFPS
                     DYAAATVPSVPSVACSTPEALAWDASFRNLNEPSGRAVKTVHQRGFN"
     gene            complement(4438..4959)
                     /locus_tag="PLG42_07875"
     CDS             complement(4438..4959)
                     /locus_tag="PLG42_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31165.1"
                     /translation="MKNKVIGILAFAVIAAFIMPSAVNAQAKANFSGNWVFNASKSDQ
                     PPGGQGGGQGQRGGFGGGDFVAKQDANTLTVERTRTTQDGQTVTTTMKYNLDGKESVN
                     AGMGGRGESKSVATWSADGKTLTIVTTRTFNDRTMTTKEVWTLTTPTQLTITTTTQTP
                     NGEVTMKRVYDKK"
BASE COUNT         1505 a         1010 c         1185 g         1485 t
ORIGIN      
        1 catacaaaaa tccgatattt gaagggaaga gaatcgttca cggacatcgt ccgaaacaac
       61 ttgaatatac taaaaaatta gttgaagaga attcgcatgt gttaccaatt gacacgggat
      121 gtgtttacgg aagggagatg aattatggtt atcttactgc tctggatgtg aattcaatga
      181 aacttatttc aattgaaaat attgattggg aatagtattt ccaccaggat gactttattc
      241 tcaggtagtt ataatcgcaa gagcatattt ttaaaattat aagcatgaat tttaaaatcg
      301 aaagattact tttaaacttt gaaatattag aggttaagtt ctaaaaagag atttcatgaa
      361 aaggcttgtt tttattgttt tttcagtgat ttttacagaa tcatttttat tccctcagca
      421 acctgtattt accaggcagg atactttgag gggctcaata acccctgaaa gggaatggtg
      481 ggatctggtt tattaccatc tgaatattaa cgtaaatcct gccgacagca caatttctgg
      541 cacaaacact gtggtataca gggtgcttaa gagtcataca gtgatgcaga tagaccttca
      601 gcctccctta aggctcattt cagccacaca agacggccgg aggcttaaaa tagagcagga
      661 gggaaatgct cactttattc atcttgagaa gaagcaggaa gccggacaga ttaattccat
      721 agtgcttgag tacggtggca ggccaagaat tgcccccaac ccgccttggc agggtggtat
      781 tacctggaaa tatgacagaa ggggtaatcc cttcatagcc tcttcatgcc agggcgacgg
      841 cgccagcctg tggtggccat gcaaagatca tatgtacgat gagccagata gtatgctcat
      901 aagcgttact gttcctgcaa accttgtgga tgtttcaaac ggccggctga ggagtgttac
      961 agaaaacaaa aagaacagga caaaaacatg gaactggttc gtttcaaacc cgataaataa
     1021 ttatggtgtg aatataaata tcggtgatta cgtgcacttt tcggaaaaat tcaatggaga
     1081 aaaaggagtg ctcgattgtg attactgggt tctgagagat aacctggaga aggcaaagga
     1141 gcatttcaaa caggtacaac taatgcttaa agcttttgag tactggttcg gcccatatcc
     1201 tttctacgaa gatggttata aactggttga ggcgccatac ctgggcatgg agcatcagag
     1261 ctcagttact tacggaaacg gataccagaa tggatacctg gggcgagata tgagtggcac
     1321 cgggtggggc gactcatggg attttataat tgtgcacgaa tcgggtcatg aatggtttgc
     1381 aaacagcatc acctacaaag atattgctga catgtgggtt catgaaagct ttataaatta
     1441 ttccgaaagc ctctttgtgg agtattacca cggtaaagaa gccggagctg aatatgtcag
     1501 aggcacgaga agaagcatac gtaacgacag gcctattgtt ggaatttata atgtcaacta
     1561 cgaaggttca ggcgatatgt actctaaagg tggcaatatg ctccatactt tgaggcaggt
     1621 catcggcgac gatgataaat ggagggagat actcagaggg ctgaacagtg agttctggca
     1681 ccagactgtc gatggttcac agatagaaga atatatcagc aaaaaagccg gaatgaatct
     1741 gaaacctttt tttgaccagt atctgaggga cgtcagaatt ccggtttttg agtattatgt
     1801 aaaagataaa agtctgacat tcagatggaa caactgtgtt cagggtttta atatgccttt
     1861 gaaaatatat gtaaataaga agcctttgat tatcaatcct tcagcacgat ggacaaccat
     1921 aacacttgaa gagccagctt ctgaaataaa agttgacagg gactattatg tagcagtact
     1981 tgatatgatg ggcaattgat ttatgatttg aggaatcagg caagtcgttt tacataaatg
     2041 tatttatcat cacccctatc ataataatct tttataatcg cttcggggat atatcctgat
     2101 ttgctgtaaa aggctctggt gggagcatac agctcacgtc cggatgtctc tgctacaatc
     2161 attgtaccac ccattttctt tacctgtgaa tgggtctcct caagaagcct gctgccgatt
     2221 cctttcccca tgaagttttt atgggttaca atccagaaaa gcagaaatgt ggattttgtc
     2281 acagggtcgg gtccgtaaca tgtatatccc actgtttcgc cttctgactc tgcaaaaaca
     2341 aaatagtagc ccgattcttc tcctttttgc agtcgttcct taattaattc gacagcaact
     2401 tctgtttcaa aatcatggaa aaaacctgta gagctcacaa tttcttcaat gcgctttaca
     2461 tcatcttttt ccggtatggt tcgaaatgtg agattcatta ctaaatgatt aaccctcaaa
     2521 aatatgaata tattttgatg gtggaaagaa gatttataaa aataaaaaat ttattttttt
     2581 aagtaaaatg tttaaaatat aaaattaatc aatatatttg cttgaaaaat tttattaaaa
     2641 gaatttaata agtataaatc attaaacgta ttttctatgt gtggtataat aggagtgttt
     2701 gacttgaagc aaagccctga agagctcagg cctcaggtgc ttacaatggc aaagaagata
     2761 aggcacaggg gacccgactg gtcgggtatc tacagcagtg acagggttat tcttgcccat
     2821 gaaaggcttg ccatagtaga ccctatttca ggtgcccagc ccataaagag cagcgatgga
     2881 aaactagtcc ttgcaattaa tggcgagata tacaatcata acgaattaag aaaaaacctg
     2941 aaacaggatt acaatttctt aaccaggtct gattgtgagg tgattcttgc cctctaccgt
     3001 gaaaaaggtg tcaattttct taatgagctt aacgggatct ttgcgtttgc attatacgac
     3061 agtgaaaaag atgtctattt tattgcccgg gatcatattg gaatagttcc attatacaaa
     3121 ggatgggaca ggtcagggaa tttttacgtt gcttcagaac tcaaagccct tgaaggagta
     3181 tgtaataaaa ttgaaatttt tcagccgggt cattattacc tgagcagtga aggagtagaa
     3241 aagaggtggt ataatccgga atggaaatcg tatgaagcgg tgaagaacaa cacatctgat
     3301 cttcatcgtc tgaggaaggc tctcgaagat gctgtacacc gtcagctgat gtcggacgtt
     3361 ccctatggcg ttctgctttc gggagggctc gattcttcgg taatttctgc tcttgcaaga
     3421 aagtttgcag caatgagggt cgagactcag gatcttcagg aagcgtggtg gccaaggctc
     3481 cattcctttt cggtcggtct caagggttcg cccgaccttg atgcagctag aaaagtggca
     3541 gaacatatcg ggactgtcca tcatgaagtg atatttacaa tacaggaagg tctggatgcg
     3601 atttcggatg tgatttatca tattgaaaca tacgatgtta caactgtcag ggcctccact
     3661 ccaatgtatc tccttgcccg cgccataaaa tcaatgggtg tgaaaatggt tctgacaggt
     3721 gagggtgccg acgaagtttt cgggggctat ctttattttc ataaagcgcc cgacgccaga
     3781 gcatttcatg aagagacagt aaggaaaata tttaaacttc atctttatga ttgcctgagg
     3841 gcaaataaat cactcgctgc gtggggtgtt gagggacgtg ttccgtttct cgacagggaa
     3901 tttctcgacg tggcgatgac gcttaatccc gaggacaaaa tggtgaaaaa cgggcggctt
     3961 gaaaagtgga ttttaaggaa aacctttgag gatattctgc ctgagagtgt tgtatggcgt
     4021 cagaaagagc agttttctga tggtgtagga tacaactgga ttgacactct taaggagata
     4081 gccaaccgga aagtaacgga tgcacagatg cggactgctg cagaaagatt tcctgttaat
     4141 cctccgctta caaaggagga gtatctttac aggtctattt tcagccagca ttttccttca
     4201 gattatgctg ctgccacagt tccatcagta ccttcggttg cctgcagcac acccgaagcc
     4261 cttgcatggg atgcatcatt ccgcaacctt aatgaacctt caggcagggc agtaaaaaca
     4321 gtacatcaga gaggatttaa ctaagaggca agcatgcctg aagaaaaaaa agaggcttac
     4381 tgcaggcctc ttttttgttt atccgttgaa aaaataagaa tgagtttatg actttaatta
     4441 tttcttgtca taaaccctct tcatagtaac ctctccattc ggtgtctgtg tggtagtggt
     4501 gattgtaagc tgggttgggg ttgtcagcgt ccacacctct ttggttgtca tggttctgtc
     4561 gttgaaagtg cgtgttgtaa cgatggtaag tgttttaccg tcagccgacc atgttgccac
     4621 tgatttgctt tcacccctgc cacccatgcc ggcatttacg ctctctttac cgtcgaggtt
     4681 gtatttcatg gtggtggtta ctgtctgtcc atcctgggta gttcttgtcc tttcgacagt
     4741 aagtgtgtta gcgtcctgtt tggcaacaaa atcgccgcca ccgaaaccac ctctctgtcc
     4801 ctgtccacca ccctgtccac caggaggttg atcactcttt gaagcattga atacccagtt
     4861 gcccgagaaa tttgcttttg cctgtgcgtt tactgctgaa ggcattatga atgccgcaat
     4921 aactgcaaat gccagaatac cgattacttt gttcttcata gttttttgtt ttggttgtta
     4981 atttataaat agacaattaa ccaaagttat ttattcccat tatacaatgt tccgtattaa
     5041 caaattttaa tattttgttc tggttgtatt tatgtactca cccccctggg aggtaggacc
     5101 ccccaacccc cctggcaggg gggcaccccc caacccccct gagagggggg caccccccaa
     5161 cccctctggc aggggggcac ccccc
//
LOCUS       DAOSNB010000185         5162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16222_length_5162_cov_5.030938, whole genome shotgun sequence.
ACCESSION   DAOSNB010000185 DAOSNB010000000
VERSION     DAOSNB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16222_length_5162_cov_5.030938"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..266
                     /locus_tag="PLG42_07880"
     CDS             36..266
                     /locus_tag="PLG42_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31166.1"
                     /translation="MKKKLVFWMFIMFVAWSFTSCEDLFQKCKLCKTVTYENGTVISE
                     TDEVEYCGADLVKKEATPPITVGNLTTKVECR"
     gene            complement(263..2158)
                     /locus_tag="PLG42_07885"
     CDS             complement(263..2158)
                     /locus_tag="PLG42_07885"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOU31167.1"
                     /translation="MGGKRSLLKKLILLLFVCMLPVVIFSQTGFDNPTRALYIFDLAR
                     YIDFGPGFKDSSSFKIGVLLGDYELIDEMMKLAKTRTRIQDKPVRIAGFKNLESLTHC
                     QVLYVNKNAGFDLKKIKEKIAGQQTMLITEGYEFRESMINFIVVEGKPRFDINEEYIK
                     NEGMSVPESLLLTAIKTKEDWENLYELASKEIEVQKETIRQQLETIEAQRIEIMRQKN
                     LLDSLDREITIKEKELKEKQKILNTQLSQISRQQGEISAQRQLIIQQQKEVQTQKDTL
                     AAQKAKIDTQLALINQQLSKIEEQEGKIKIQLQTLEKQRLVIYFVIVALILVGVLAYF
                     IFRAYKIKKDANIALEEKNRLIMAQKDEIERQRDIAAAQRDQIAYQKKHITDSIMYAK
                     RIQAALIPSLELFSDKLEHFVLYKPLAIVSGDFYWVSTKDNLQVIIAADCTGHGVPGA
                     FMSMLGVALLNEIVNEKNIFMPDQILNRLREEVIKSLKQVPDEESVKDGMDAAVCTID
                     FNNNVLYYSGANCPLYLIRGTELIHYRADKMPVAIHYKMQPFTLNTIELQKGDAFYIF
                     SDGYADQFGGPAQKKFMTGKLKETLLEIASKPMLEQGEILDRIFEEWKGDNPQVDDVT
                     LIGVRYN"
     gene            2549..2971
                     /locus_tag="PLG42_07890"
     CDS             2549..2971
                     /locus_tag="PLG42_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOU31168.1"
                     /translation="MSLIYNNLELKGKNIIIVEDDIPSVKYYKTILENSGAELKVFEN
                     GKDFTDYLNNTEEHIDIVLLDFLIPIINGIDCVRVLRKTNRNSPVLMITAYYSESTRS
                     EALIAGCNEYILKPIYPEKLLCLLEKYLKPEHSYSVSR"
     gene            complement(2983..>5162)
                     /locus_tag="PLG42_07895"
     CDS             complement(2983..>5162)
                     /locus_tag="PLG42_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU31169.1"
                     /translation="TAIIVIRSSANPPLDENNPENPFNLLYFLNREQYGSRPLLKGPY
                     FNAPVLNYHDGKPKYSVVDGKYKVTSHELEREYDKRFITFFPRMWSDQSDHQQVYEEW
                     SRMKGIPIQVTDSDGGKKVIRKPRFIENLRFMFSYQLDFMYFRYFMWNFSGRQNDTQS
                     YGGAANGNWITGINFLDEPRVGSIKEMPEEMKKDPSRNVYYMLPFILGLIGLIYHFGK
                     DYRDWWIIMLLFIMTGIAIVIYLNQYPNQPRERDYAYVGSFYFFAVWIGLGVLAVFEW
                     LSKIMNEKIASSISGVVCFAAVPLIMGTQNWDDHDRSGRYLARDVASNYLNSCAPGAI
                     IFTNGDNDTFPLWYAQEVEGVRTDVRVCNLMLLNTDWYIDQMKHKTYESDPLPISLPS
                     YKYYDGVNNQVFIVEKTKDPVNIKTIIDWVMSENEATKIRISPTEKLDIIPSRTIRIP
                     VDTAAVIASGTVKKEDADKIVPYIDIKLKGNSIMKSQLIILDILANNNWKRPIYFVTG
                     YHNDAMGLEEYFQLEGLAYRLVPIRSHNNSWLDYGRVDAEILYNNMMNKFVWRGAKEE
                     NVYLDYNHKRTLIVVKARLNYARLAKELASQGQKEKALQVLNYCMETFPANKVPYDMY
                     IPDIIEAYYLAGDSNKAIELTKNMSDYYFKRLDYYLKQTVDIMASADYEIQSAIQFVS
                     RAADAASKNGQEEFAKEINDRLNIYYGNYIKILQSDLSDLKIK"
BASE COUNT         1428 a          994 c          992 g         1748 t
ORIGIN      
        1 tttaactttg tataataatt ttctggaggg ttgacatgaa aaagaaactg gtattctgga
       61 tgtttataat gtttgtagcg tggtcgttta cctcgtgtga ggaccttttt cagaagtgta
      121 agttatgtaa gaccgtaacc tacgaaaacg ggactgtcat aagtgaaacg gatgaggtag
      181 agtattgcgg tgctgatctg gttaaaaagg aagccacacc accaataacg gttggtaatc
      241 ttacaacgaa ggttgagtgt cgttaattgt accttacacc tattaaagta acgtcgtcaa
      301 cctggggatt atctcctttc cattcttcga atattctgtc aaggatttct ccctgctcca
      361 gcataggttt tgaggctatt tccaacaggg tctctttcag tttgcctgtc ataaatttct
      421 tctgcgcagg gccaccgaac tggtcagcat atccatcgga aaatatatag aaagcatcac
      481 ctttttgcag ctctattgta ttgagggtga agggttgcat tttataatgt atggcaacag
      541 gcattttatc agctctgtaa tgtatcagct cagtgcccct gataagatag agcggacaat
      601 tggctccgga atagtagaga acattattat taaaatcgat tgtgcataca gctgcatcca
      661 ttccatcttt caccgactcc tcgtcgggta cctgcttcag tgattttatg acttcttccc
      721 tgagtctatt gaggatctgg tcgggcatga agatgttttt ctcatttact atttcgttga
      781 gcagtgctac tccaagcata ctcataaatg caccaggcac tccatgacca gtacaatctg
      841 cagcgataat aacctggagg ttgtctttgg tgcttaccca gtagaagtca ccgctcacta
      901 ttgcaagagg cttatataat acaaaatgtt ccagtttatc actgaaaagt tcaagtgatg
      961 ggataagagc agcctgtatg cgttttgcat acatgatgct gtcggttata tgttttttct
     1021 gataggcaat ctggtcacgc tgggctgcag caatgtcacg ctgtctttca atttcatctt
     1081 tctgtgccat tatcaaccgg ttcttctcct caagggcgat gtttgcatct ttctttattt
     1141 tgtaagccct gaagataaag taagcaagaa ctccaaccag tatcagagca acaattacga
     1201 aataaattac cagccgttgt ttctcaagag tctggagctg tatttttatt tttccttcct
     1261 gctcctctat tttgcttagc tgctggttta tcaatgccag ctgtgtatct atcttagcct
     1321 tctgggctgc caatgtatcc ttctgagtct gaacttcttt ttgctgttgg atgattaatt
     1381 gacgttgtgc agaaatttct ccctgttgcc tcgagatctg gctaagctgt gtattaagaa
     1441 tcttttgttt ttccttaagt tctttttctt taattgtgat ttcacggtcg agactgtcaa
     1501 gaagattttt ctgtctcata atttcaattc tctgtgcctc tatggtttca agttgctgcc
     1561 ggatggtttc tttctggact tcgatttctt ttgaggcgag ttcatagaga ttctcccagt
     1621 cttcttttgt tttgatagca gttagcaata agctttctgg aactgacatt ccttcatttt
     1681 tgatatattc ttcatttatg tcgaatctgg gcttgccctc aaccacaatg aaattaatca
     1741 ttgattctct gaattcatag ccttctgtta ttagcattgt ctgttgcccg gcaatctttt
     1801 ccttgatttt tttaaggtca aagcccgcat ttttatttac gtaaagcacc tggcaatggg
     1861 taaggctctc aaggtttttg aaaccagcta ttcttacggg tttatcctga attcttgttc
     1921 ttgttttagc taatttcatc atttcgtcaa tcagttcata gtcaccaagg agtacgccga
     1981 ttttgaatga tgaggaatct ttgaatccgg gcccaaaatc aatatatctt gccagatcaa
     2041 aaatatagag ggctctggta gggttatcaa atcccgtctg cgaaaaaatg acaaccggca
     2101 acatgcaaac aaaaagcagc aggattaatt tctttaaaag agatcttttt ccacccatgg
     2161 taggagatat attttttacg aaattgctaa atattattca gttatgcaag tgcaggaaga
     2221 ttattgagga taaataatta ttctgcctga cctgctttta ttaaaataaa aggataattt
     2281 aaactttgac cgtttcagga ataacggatg atgttcttgc aaagaattaa gcataaatat
     2341 tagataatta ataagatagg gcaaaaatgg gcaatcatag acactttaaa actctactat
     2401 cgaaacgata gttaagtaac aatttgataa taagttgttt ataagaaatt aaaaccaaat
     2461 tgttgataaa ttgttaatat ttgacacttt catcgatttt cagcatagat tgttatcgta
     2521 attttattct gaaaatttct ttcaaagaat gagtttgata tacaacaacc tggaattaaa
     2581 aggtaaaaac attataattg ttgaagatga cattccttcc gtcaaatatt acaaaacgat
     2641 acttgaaaat tccggtgccg aattaaaggt ttttgaaaac ggaaaagatt tcacagatta
     2701 tttgaataat acagaagaac acatagatat tgtgctgctc gattttctta taccaataat
     2761 caatggaatc gattgtgtaa gggttttaag aaaaactaac cggaattcac cggttttaat
     2821 gattacggcc tattattctg agagtacaag aagtgaagct ttgattgccg ggtgcaatga
     2881 atatatactg aaacctatat atccagagaa gctattatgc cttcttgaaa aatatctaaa
     2941 accggagcac tcttattctg tatcccgtta attacttttg attcatttta ttttcaggtc
     3001 tgacagatct gactgtaaaa tttttatata gtttccgtag tatatattca gccggtcgtt
     3061 tatttccttt gcaaactctt cctggccgtt cttgcttgct gcatctgctg ctcgtgaaac
     3121 aaattgtatt gccgactgta tttcataatc agcagaagcc ataatgtcga ccgtctgctt
     3181 cagataataa tcgagacgtt tgaagtagta atctgacata ttttttgtca gttcaatggc
     3241 tttattgcta tctcctgcca ggtaatatgc ttctataatg tccgggatgt acatatcata
     3301 tggtactttg tttgcaggga atgtttccat gcagtaatta aggacctgaa gtgccttttc
     3361 tttttggccc tgggaagcaa gttcttttgc cagtcgtgca taattaagcc gggctttgac
     3421 aacaatcaat gttcgtttat gattatagtc gagatataca ttttcttctt ttgcccctct
     3481 ccatacaaat ttattcatca tattgttgta aaggatttct gcatcaactc tgccgtagtc
     3541 gagccagctg ttattgtgcg accttattgg taccagccgg taggcaagac cttcgagctg
     3601 gaagtattcc tcaaggccca tggcatcgtt atggtatcct gtaacgaaat agatgggtct
     3661 tttccagtta ttattggcaa gaatatcgag gatgatgagc tggctcttca ttatggaatt
     3721 tcctttcaat ttgatatcta tataaggaac tattttatcg gcgtcctctt tttttactgt
     3781 acctgaagca attactgcag cagtatctac aggaattctg attgtacggg aaggaattat
     3841 atcaagtttt tctgtcgggg atatccgtat cttggttgcc tcgttttcac tcattaccca
     3901 gtcgattata gttttgatgt taaccgggtc tttggttttt tctactataa aaacctggtt
     3961 gttaacgccg tcgtaatatt tgtatgatgg aagtgaaata ggaagaggat cagattcata
     4021 agttttatgt ttcatctggt cgatatacca atcggtattc aaaagcatca gattacatac
     4081 ccttacatca gttctgactc cttccacttc ctgggcatac cagagtggga atgtgtcgtt
     4141 gtcaccattt gtaaaaatta tggcaccagg agcacatgaa ttgaggtaat tggaagccac
     4201 atcccttgca agatatcgtc ccgacctgtc gtggtcgtcc cagttttgtg ttcccattat
     4261 aagaggtaca gctgcaaaac agacaacacc tgatattgag cttgcaattt tttcgttcat
     4321 gattttcgaa agccattcaa aaactgcaag gacccccagc ccaatccata cggcaaagaa
     4381 atagaatgag cccacgtacg catagtccct ttctctcggc tggttcggat actgattcag
     4441 gtaaatcact attgcaatac ctgtcattat aaacagcagc ataattatcc accagtccct
     4501 gtaatctttt ccgaaatgat atatcagacc aatgagaccc agaataaagg ggagcatata
     4561 gtaaacattt cttgaaggat cttttttcat ttcttcaggc atttctttta tacttcccac
     4621 gcgtggctcg tcgagaaaat taattcccgt tatccagttt ccgtttgctg cacctccata
     4681 actttgtgta tcattctgcc ggcctgagaa attccacatg aaatatctga agtacataaa
     4741 atcgagctgg tacgaaaaca taaaacgaag gttttcaata aatctgggtt ttcgtattac
     4801 tttcttccca ccatcagaat cagtaacctg gatcggtata cctttcattc ttgaccattc
     4861 ctcataaacc tgctggtgat cgctctggtc gctccacata cgcgggaaaa aggttataaa
     4921 tcttttgtca tattcccttt caagctcatg acttgtaact ttatatttcc catctacaac
     4981 actgtatttt ggttttccat cgtggtaatt caatactggc gcattgaagt aggggccttt
     5041 aagtaatggc cttgagccat attgttcacg gttaagaaaa tagagaaggt taaaaggatt
     5101 ttcaggattg ttttcgtcga gtggaggatt ggctgaagaa cgtataacaa ttattgctgt
     5161 gg
//
LOCUS       DAOSNB010000186         5147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16294_length_5147_cov_4.485271, whole genome shotgun sequence.
ACCESSION   DAOSNB010000186 DAOSNB010000000
VERSION     DAOSNB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16294_length_5147_cov_4.485271"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLG42_07900"
     CDS             complement(<1..406)
                     /locus_tag="PLG42_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31170.1"
                     /translation="MKEKVIAVFDIGKTNKKLLLFDTQLNVLKETEEKLVATTDDDGF
                     ECDDIELIEKWVKNSLGEVIQSEKYDLLAVNFATYGATLAYIDSHGRRLTPIYNYLKP
                     VDESIPEELYRKYGGKEEFCRRTASPALGMLNS"
     gene            complement(425..1693)
                     /locus_tag="PLG42_07905"
     CDS             complement(425..1693)
                     /locus_tag="PLG42_07905"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOU31171.1"
                     /translation="MKKVDIERAYKAAKEQYAALGVDTDKVIEQMSNIPISLHCWQTD
                     DVGGFEKPGAELGGGGIQVTGNFPGKARTIQEMRQDLEKVFSLLPGKLRLSLHAIYGE
                     FGGKLVDRDQIETKHFQGWIDWAKKKGIGLDFNCTCFSHPYADDGYTLSSRNEKIRKF
                     WVEHVKRCRKIAADMGKQLGIPAVHNIWIPDGSKDTPVDRSTLRKILKKSLDEIFSVK
                     YPKKYLKDSVESKLFGIGSESMVVGSHDFYLGYAVKNNIMITLDNGHFHPTEQVGDKI
                     SSILLYVDEILLHLTRGVRWDSDHVLTLNDELILIAQEIVRAGALNRVNIGLDFFDAS
                     LNRIGAYVVGTRSAQIAFMYALLEPLKMLNEFEIKGKNFERLAYLELMKTKPFGAVYD
                     YYCMKSGKPAGEDYIQEIQKYEKDVLLKRK"
     gene            complement(1762..2076)
                     /gene="rhaM"
                     /locus_tag="PLG42_07910"
     CDS             complement(1762..2076)
                     /gene="rhaM"
                     /locus_tag="PLG42_07910"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOU31172.1"
                     /translation="MKRFGFKMKLLPGCREEYKRRHDAIWPELVKLLKDNGICNYSIF
                     FDEETNTLFAYQEQTGESSSQDLGNTEIVQKWWKYMADIMETNPDYSPVSVPLEQVFY
                     ME"
     gene            complement(2088..3116)
                     /gene="rhaT"
                     /locus_tag="PLG42_07915"
     CDS             complement(2088..3116)
                     /gene="rhaT"
                     /locus_tag="PLG42_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOU31173.1"
                     /translation="MNPIAGIFLIMLGSIGAASFYVPFKKVKSWAWESYWISQGLFAW
                     IIIPWIFAVIFIPRGELMPIIREAPSSAKLMAAFFGLLWGFGGLTFGLSIRYLGIALG
                     QSIALGLCAAFGTLIPPIVAGQNLFATTSGILTVIGVAITVAGIAIIGYAGSLKSAEM
                     SEEEKKAAVKEFALKKGILIAIFSGLMSACFNFGFEAGKPIEKIALEHGTNPLFQKNP
                     TLIFILLGGFITNLFYCGFLNIKNKTWKDYINVAPSVFLNNLFFTFLAGLLWFLQFHF
                     FGMGSSKLPEGMAVFGWSILMALNIAISNIWGILLKEWEGVRRKTIVVLVTGIVILIL
                     STFIVKLG"
     gene            3261..>5147
                     /locus_tag="PLG42_07920"
     CDS             3261..>5147
                     /locus_tag="PLG42_07920"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOU31174.1"
                     /translation="MKKIILSLVFSAFIINSLSAQELSVYGLTCEHKVNPLGVDTQKP
                     RFSWKISSTGKNVMQTAYFIQVSTDPKFSPGKIVWQTGLVQSDESVLVPYGGSRLEPC
                     RRYFWRVRIKDNSGKESKWSETAFFETGLMSPSGWKAKWIEKEGDTLRYSPSPHFRKE
                     FTITKPVAKATVYVTSHGFYELHLNGSKVGDQVLTPGWTSYGKRLQYQVYDVTNQIIK
                     GKNAVGAVLGDGWYRGTLAWSNNWAIYGKTLGLLMQLRIVHTDGTETMIITDESWKAN
                     QDGAIRMNDIYNGEIYDARKRLKGWNLPGYDESNWVAVKVANYGYDNIIATEGVPVRK
                     IQEIKPVKILRTPKGSLVVDMGQNMVGWVRLKVKGERGTVVVLRHAEVLDKYGEFYTA
                     NLRSAKCQLTYTLSGEGEEVYEPRFTFMGFRYVEVNGFPGELTTDNITGIVVHSDMPV
                     TGSFECSNPLVNQLQHNIQWGQKGNFVDVPTDCPQRDERLGWTGDAQVFCRTAAFNMD
                     VAAFFTKWLKDVAADQKPGGEVPDVIPDVLNPQGAKTAAPSAAWGDVAVITPWTMYLV
                     YGDKQLLETQYPSMKAWVEYIRNKAGDSYIWKGGSKYGDWLFYHPPVNRHTEFDGHTE
                     HDFIA"
BASE COUNT         1537 a         1055 c         1026 g         1529 t
ORIGIN      
        1 ctgaattgag catacctagt gctggagagg cggttctgcg gcaaaattct tcttttcctc
       61 catactttct gtatagttct tctggtattg attcatcaac aggtttcaaa tagttataaa
      121 taggtgtcag acgcctgcca tggctatcga tataagcaag tgttgcaccg taagtagcaa
      181 aatttacagc cagtaaatca tatttttcag attgaataac ttcgccgagt gaatttttaa
      241 cccatttttc aataagttca atatcatcac actcaaatcc gtcatcatca gttgtggcaa
      301 ctaatttttc ttcagtttcc tttaaaacat taagttgtgt gtcaaaaaga agcagttttt
      361 tattggtctt acctatatca aaaacggcaa taactttttc tttcattccc tgattttttt
      421 aatcctattt cctcttcagc agaacatctt tttcgtattt ctgaatttcc tgaatgtaat
      481 cttctccggc tggttttccg cttttcatac aataataatc atatactgca ccaaaaggtt
      541 tagttttcat aagctcaaga taagcaagcc tttcgaagtt tttacctttt atttcgaact
      601 catttaacat tttcagcggt tcaagcaatg catacataaa tgcgatctga gcagatcgtg
      661 taccaaccac atatgctcca atgcgattca ggcttgcatc gaagaaatcg agtccgatat
      721 ttaccctgtt cagagctccg gcacgtacaa tctcctgtgc aataagtata agttcatcat
      781 taagggtgag aacatggtca ctatcccatc ttacacccct ggtaaggtga agcagtatct
      841 cgtctacata aagcaatatt gaagaaattt tgtcgcccac ctgttctgtc ggatggaaat
      901 gtccattatc gagcgttatc attatgttgt tcttaacagc atagcccaga tagaaatcgt
      961 gtgatccgac aaccattgat tcagagccga ttccgaagag tttgctttca acagaatcct
     1021 tcagatattt tttcggatat tttactgaaa atatttcatc gagtgatttc ttaagaattt
     1081 tacgaagtgt gctccggtct accggagtat ctttcgaacc atcaggtatc cagatgttat
     1141 gaactgccgg tattccaagc tgtttaccca tgtcggcagc aattttccgg catcttttaa
     1201 catgttctac ccagaatttt cttattttct catttctgct cgaaagtgta taaccatcgt
     1261 cagcgtaagg gtgcgaaaaa catgtacagt taaaatcgag tcctatccct ttctttttgg
     1321 cccagtctat ccagccttga aaatgttttg tttcaatctg gtccctgtca accagttttc
     1381 cgccgaattc tccataaatt gcatgcaggc taagccttaa tttgccagga agcaatgaaa
     1441 acactttttc gaggtcctga cgcatctcct gaatagttct ggctttaccc ggaaaattac
     1501 cggttacctg gatacctcct ccaccaagct ctgcacccgg tttttcaaaa ccacccacat
     1561 cgtctgtttg ccagcagtgt agacttatgg gaatattgct catttgttca ataaccttat
     1621 cggtatcaac tcccagtgca gcatactgct cttttgctgc tttataggct ctttcgatat
     1681 caactttttt catcttttat aatattattt atttgttgta ttatcggcgt tctgttaaaa
     1741 atttgttctt tatttcttta ctcattccat ataaaagacc tgctcgagag gaacagaaac
     1801 aggtgagtaa tcgggattag tctccataat gtctgccata tacttccacc atttctgcac
     1861 tatttcggta ttacccagat cctgtgaaga actttcgccc gtctgctcct gatatgcaaa
     1921 aagggtattt gtttcttcgt caaagaatat agaataatta catataccat tgtctttcaa
     1981 tagctttact agctcaggcc atatagcatc gtggcggcgt ttgtactcct ccctgcaacc
     2041 ggggagtagt ttcattttaa agccaaacct tttcatattt ttttcattta tcctagttta
     2101 actataaatg ttgaaagtat cagaattaca attccagtaa caagaacaac aattgttttt
     2161 cttcgtacgc cttcccattc cttcagcagg ataccccaga tattgcttat agcaatatta
     2221 agggccatca gtatactcca tccgaagaca gccattcctt caggtagttt gcttgaaccc
     2281 ataccaaaga aatggaactg caggaaccag agaagtccgg caagaaaagt gaaaaacagg
     2341 ttattaagaa atactgacgg ggcaacgtta atgtaatctt tccaggtttt attttttata
     2401 ttgaggaagc cgcaataaaa aagattggtg atgaagccac cgagtaatat aaatataagt
     2461 gtcgggtttt tctgaaagag aggattggtg ccatgttcaa gagctatttt ttcaataggt
     2521 tttccggctt cgaacccgaa attgaaacat gcactcatca atccagaaaa aattgctatc
     2581 agaattcctt tcttaagagc aaattctttt acagctgctt ttttttcttc ctccgacatt
     2641 tcagcacttt ttagcgagcc ggcataaccg attattgcaa tacctgcaac tgtaattgca
     2701 actccaataa cagttagtat accactggtt gtggcaaaca gattctgacc tgcaactata
     2761 gggggaatca gtgtcccgaa tgcagcacag aggccaagtg caatgctctg tccaagagca
     2821 ataccaagat accttatact gagtccgaat gttagacccc caaaacccca taagagacca
     2881 aagaaagcag ccatcaattt ggcagatgat ggagcttctc tgattatagg catcagttca
     2941 ccacggggta taaaaataac agcaaatatc catggtatta ttatccatgc gaagagaccc
     3001 tgggaaatcc agtacgattc ccacgcccat gatttaacct ttttgaaagg cacatagaag
     3061 ctggctgcac ctatgctacc aagcattatc agaaaaatac ctgctattgg attcatttat
     3121 aatcttttat tttaacaatg gtttattttt acgaaactaa aaataactaa tttatttttt
     3181 atctcaagca ttaagttttt tttacatttg cttatagtaa agcctgaaaa aaaaattgaa
     3241 ctctgaaaaa cttatcagct atgaaaaaaa taattctttc attagttttt tctgcattca
     3301 ttattaattc gttatcagct caggagttat cagtttatgg actgacatgt gaacacaaag
     3361 ttaatccact tggagttgat acacaaaaac caagattttc atggaaaatt tcttccacag
     3421 gtaagaatgt gatgcagacg gcatatttca ttcaggtatc aaccgatcca aaattttccc
     3481 cgggaaaaat tgtatggcaa actggactgg ttcaatcaga tgaatccgta ttggtacctt
     3541 atggagggtc cagacttgaa ccgtgcagaa ggtatttctg gagagtcagg attaaggaca
     3601 attcagggaa ggagtcaaaa tggagtgaga ctgcattttt tgaaacaggt ttaatgtcgc
     3661 cttccgggtg gaaagcaaaa tggattgaaa aggaaggaga tacgcttcgc tattcacctt
     3721 cacctcattt caggaaagaa tttacaatca caaagcctgt tgcaaaagcg acagtttatg
     3781 ttacatccca cggtttttat gaattgcatc tcaatggcag taaagtcggt gaccaggtgc
     3841 tcacccccgg ctggacgtcg tatggaaaac gtcttcagta tcaggtttat gatgtaacca
     3901 accagataat aaagggcaaa aatgctgttg gagcagtttt aggcgatggt tggtaccgcg
     3961 gtacgctggc atggagcaat aactgggcta tttatgggaa aacactcggt ttactgatgc
     4021 aactcagaat agtccatacc gacggcactg aaaccatgat tattactgat gaatcatgga
     4081 aagcaaacca ggatggcgca ataagaatga atgatatata taatggtgag atttatgatg
     4141 ctaggaaaag actaaaagga tggaatcttc ctggctatga tgaaagcaac tgggtagcgg
     4201 tgaaggtggc aaattacggt tatgataaca ttatagcaac cgaaggtgtt ccggtaagaa
     4261 agattcagga gataaaacca gtaaaaatac tacgcacacc aaaaggtagt ctggttgttg
     4321 atatgggtca gaatatggta ggatgggtca ggttgaaggt taaaggagaa cggggcacgg
     4381 tagtcgtatt aaggcatgca gaagtgctcg ataaatatgg agaattctat accgcaaacc
     4441 ttagaagtgc aaaatgccag ctcacatata ctctttcagg agaaggcgaa gaggtttatg
     4501 agccgagatt cacttttatg ggattcaggt atgttgaagt aaacggtttt ccgggtgaac
     4561 taaccacaga taacataacc gggatagtcg tgcattccga catgcctgtc acgggaagtt
     4621 tcgaatgttc aaatccactg gtcaatcagt tacagcacaa tattcagtgg ggacaaaaag
     4681 gcaattttgt agatgtcccg accgattgtc cgcaaaggga tgagcgtctg ggctggacgg
     4741 gtgatgcaca ggtcttctgc cgtacagctg catttaatat ggacgtagct gcattcttca
     4801 ccaagtggct gaaggatgtg gcagccgacc agaaaccggg cggagaggtg ccggatgtca
     4861 tacctgatgt acttaatcca cagggagcta aaacagcagc accttcggcc gcatggggcg
     4921 acgtggcagt aatcacaccc tggacaatgt atctggttta tggtgataaa caactgctcg
     4981 aaacccaata tccaagcatg aaagcatggg ttgagtatat tcgcaacaaa gccggcgaca
     5041 gctatatctg gaaaggaggc agcaaatacg gcgactggct tttttatcat ccacctgtaa
     5101 acaggcatac cgaatttgat ggacataccg aacacgactt catcgcc
//
LOCUS       DAOSNB010000187         5126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16404_length_5126_cov_4.211990, whole genome shotgun sequence.
ACCESSION   DAOSNB010000187 DAOSNB010000000
VERSION     DAOSNB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16404_length_5126_cov_4.211990"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1777)
                     /gene="ggt"
                     /locus_tag="PLG42_07925"
     CDS             complement(89..1777)
                     /gene="ggt"
                     /locus_tag="PLG42_07925"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOU31175.1"
                     /translation="MRKAGFTLFLILIPYVFMGQDRVTGRGFATRSEVIAQNGMAATS
                     HPLATQVALDILKKGGNAFDAAIAANAVLGLMEPTGCGIGGDLFAILWSAERGKLYGL
                     NASGRSPRSLKLEYFKENNIEKIPSYGPLPVSVPGCVDGWFEIHDMFGKLPMADILQP
                     AIKYAREGFPVTEIIAWQMKRNLEILKDYPNIKEVYMPDGRAPEKGEIFKNPQLASTL
                     EKIVKGGRNEFYRGSIARTIDAFMKKQGGFLSYDDLARHHSEWVEPVSTTYRGYEVWE
                     LPPNGQGIVALQMLNILEGYDIASMGFGSAEYMHLFTEAKKLAFEDRARYYADPAFSK
                     IPISRLISKKYAAERRKLIDFNKAAKVYQPGNLEPDNTIYLTVADRYGNMISLIQSNY
                     RGMGSGMCPTGLGFILQDRGEMFTLEEGHPNCYAPGKRPFHTIIPAFITKEGKPYISF
                     GVMGGDMQPQGHVQIIVNLIDFKMNLQEAGDAPRMYHTRSSEPTGEQMIDGGILNLES
                     GFRWEEIQKLLQKGHTIQWNLGGFGGYQAIMWDEKNKVYYGASESRKDGQAAGY"
     gene            2005..4056
                     /locus_tag="PLG42_07930"
     CDS             2005..4056
                     /locus_tag="PLG42_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU31176.1"
                     /translation="MRKINILIMLTALMLLASCTGRQTKTEEGEEFNYFVDQFEDMRV
                     FRYRLPGFESLSLRQKELIYYLSEAALCGRDILWDQNFKYNLLIRKTNEAIIKSWQGD
                     KETPEYKSFITWAKRVFFANGIHHHYSADKMLPSFSKEFYTSLVKGSDQSLLPVGNGK
                     TADDLLAVILPVIFDPDLYPRKIERGEGKDIIAESAVNFYEGVTAREVESFYNGKINP
                     ADPRPVAIGLNSKLVKKNGRIVEEVYKSGGLYGEAIDRIIYWLEKAKNVAENEIQKKE
                     LEVLIQYYRTGDLKTWDDYNVLWARNTEPVVDYVNGFIEVYEDPMGMKATWEAIVDYK
                     DPEATRRTETITANAQWFEDNSPVQPQYKKEKVTGVAAKVINIAMLGGDCYPASPIGI
                     NLPNSDWIRKEVGSKSVTLANISEAYDVMSARSGMLDEFAASKEEIERVRKYASIADA
                     LHTDLHECVGHASGKLAEGTDPNALKEHQSALEEARADLFALYYMMDNKLIELGLLPE
                     KEAAMAEYDSYIRNGMLTQLVRIRPGKQIEQAHMRARAMIARWAYENGKDENVIELFT
                     RDGKTFVKINDYEKLRNLFGSLLREVQRIKSEGDYEAGKTLIESYGVKVDPVLHNEML
                     ARYEKLKLAPYTGFLNPRLVPVYNDKGEITDVKVEYEQDYLSQMIYYGTNYSFLPAIL
                     N"
     gene            4095..>5126
                     /locus_tag="PLG42_07935"
     CDS             4095..>5126
                     /locus_tag="PLG42_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOU31177.1"
                     /translation="MKEIKLLASAIILAAASSTIAQVPEAVLWKKYELTFPSTVTYDN
                     PVQDIKTFQVTFTSPTGRVKTINGFWDGGSTWKARFMPDETGDWKWETTCSDTKNAGL
                     HNVKGEFRCIPNNGKSDLDIHGRIIRQPGLFYLTHADGTPFLYIGCTAWNGALKSTDS
                     EWDQYLKHRAENQYSVIQLVTTQWRGCDKNSLGQVAFEGSARIRINPEFFRHLDKKID
                     RVNDYGLVAAPVILWALQTGAGRELSPGYYLPEDQAILLAKYIVARYGANHVIWLLGG
                     DGIYTGIYEQRWKTIGRAVFDGTHQGIVAQHPCGRSWIGEIYRDEEWLDIVGYQSSHS
                     NDKGTVEWIT"
BASE COUNT         1540 a         1026 c         1133 g         1427 t
ORIGIN      
        1 tgtgcttcac agcaaaaacg gtttttaaat atgagcctac cgactttggc ttccactcaa
       61 attctggctc caatcatact tcttatatct aataaccagc cgcctgcccg tctttcctcg
      121 attcggaagc cccgtagtag accttgttct tttcgtccca cattatagcc tgataacctc
      181 caaaaccacc taaattccac tgaatggtgt gacctttctg cagtagcttt tgaatctctt
      241 cccacctgaa cccgctctct aaattaagaa ttcccccgtc aatcatttgc tcacctgtag
      301 gttccgacga acgggtgtga tacatcctgg gcgcatcgcc tgcctcctga agattcatct
      361 tgaaatcaat cagatttaca ataatctgta catgaccctg tggctgcatg tcgccaccca
      421 tcacaccgaa gcttatgtaa ggcttgcctt cttttgtgat gaaagctggt ataattgtat
      481 ggaacggcct tttcccgggc gcataacagt tgggatgacc ctcctcaagt gtaaacatct
      541 caccacggtc ctgaaggata aaaccgagtc cggtcggaca catacccgat cccatgcccc
      601 tgtaattgct ctgtataagc gatatcatat tgccatatct gtcagcaacg gtcagatata
      661 ttgtgttatc cggctcgagg ttgcccggct gataaacttt ggcagcttta ttgaagtcaa
      721 taagctttct tctttcagca gcatattttt ttgagatgag ccgactgatg ggtatctttg
      781 aaaaggcagg atcggcataa tagcgcgcac ggtcttcaaa agccagtttc ttggcttcgg
      841 taaagaggtg catatattca gcagagccaa accccataga agcaatgtcg tagccttcaa
      901 gtatattgag catttgcagg gcaacaatcc cctgaccatt aggaggtaat tcccatactt
      961 cgtaacccct gtaagtggta cttacaggtt caacccattc cgaatgatgt cgtgcaagat
     1021 cgtcatatga aagaaaacct ccctgttttt tcatgaatgc gtcaatcgtg cgggcgatgc
     1081 ttcccctgta aaattcattt cttcctccct ttacaatttt ttcaagtgtg cttgccagct
     1141 gagggttttt gaaaatctct cctttttctg gtgctcttcc gtcaggcata taaacctctt
     1201 taatattcgg ataatccttc aaaatttcca gattgcgctt catctgccat gcgataattt
     1261 ctgtcaccgg aaacccttct ctggcatatt ttatggcagg ctgcaggatg tcagccattg
     1321 gcaattttcc gaacatatcg tggatttcaa accagccgtc gacacatcca ggtacggaaa
     1381 ccggtaaagg tccgtaagat ggaatttttt caatattatt ttctttgaaa tattcaagct
     1441 tcagcgacct tggtgagcgt ccactggcat ttagtccgta aagcttacct ctttccgcac
     1501 tccagagaat ggcaaaaagg tctcctccta tgccgcaacc tgtgggctcc atcaacccca
     1561 gaacagcatt ggcggcaatt gcagcatcaa aagcatttcc gccttttttc aggatatcta
     1621 aagcaacctg agttgcgagc ggatgactcg tagcagccat accattctgt gcaatcactt
     1681 ccgaacgcgt tgcaaatcct cgtccggtca ctctgtcctg acccataaaa acatatggta
     1741 ttaaaatgag aaataatgta aaacctgctt ttctcacatc aaaatcattt gaagacaaaa
     1801 ttaagaatat aacataatat ggtgtttaaa ctctttgtgg aaaattgaca ggatttatat
     1861 tttttacgat tgatttttgt tgattatttt ttgaagggca tcagaaagat tttctgcttt
     1921 ttgctttcaa ttctcatttg gaaatagtgg tttttaaagg ctttttaata cttttggact
     1981 cattaattca aaatatttgt aatcatgaga aaaataaaca tattaattat gcttacagct
     2041 ttaatgcttc tggcttcatg cacaggcagg caaacaaaaa ccgaagaggg agaggaattc
     2101 aactattttg ttgaccaatt tgaggatatg agggtattca gataccgtct tccaggtttt
     2161 gaatcccttt ctctacgtca gaaagaactc atttactacc tcagtgaagc tgccctgtgc
     2221 ggaagggata tcctgtggga ccagaacttt aaatataatc ttctgataag aaagacaaac
     2281 gaagcaataa ttaaaagctg gcagggagat aaggagacac ctgagtataa atcgtttata
     2341 acatgggcaa aaagagtatt ctttgctaac ggcatacatc accattattc tgcagataag
     2401 atgctgccat cattttcaaa ggaattttat acttcacttg taaaaggtag cgaccagtct
     2461 cttcttccag ttggcaacgg aaaaactgct gatgatcttc tggcagttat tttgccggtg
     2521 atatttgatc cggacctcta tccgcgtaaa atagaaaggg gagaaggtaa agatattatt
     2581 gcagagtcgg cagtaaattt ttatgaaggg gttactgcaa gggaagtaga atctttttac
     2641 aatggaaaaa taaaccctgc cgatccaagg cctgttgcta tagggctgaa ctcaaaactc
     2701 gttaagaaaa acggcaggat agttgaagag gtttataaat ccggtggttt atacggggag
     2761 gctatcgaca ggataattta ctggctggaa aaagcaaaaa atgtcgcaga aaacgaaata
     2821 cagaaaaaag aacttgaagt gcttattcaa tattacagga caggggatct taagacctgg
     2881 gatgactata acgttttgtg ggctagaaat actgaacctg ttgtggatta cgtaaatggt
     2941 tttattgaag tatatgaaga cccgatgggt atgaaagcca catgggaagc tattgttgat
     3001 tataaagacc ctgaagcaac caggaggact gaaacgataa cagccaatgc gcagtggttt
     3061 gaggataaca gtccggttca accgcagtac aaaaaagaga aagttaccgg ggttgctgct
     3121 aaagttatca atatcgcaat gcttggagga gattgctatc ctgcttcacc tattggaata
     3181 aatctgccca attctgactg gataagaaaa gaagtgggtt caaagtcggt cactctggca
     3241 aacatttcgg aagcctatga tgtgatgtcg gcaagatcgg gtatgctgga tgagtttgct
     3301 gcaagtaaag aggagataga acgtgtaagg aaatatgcat ctattgcaga tgccctgcac
     3361 accgaccttc acgagtgtgt gggtcatgca agcggtaaac ttgctgaagg aaccgaccca
     3421 aatgccctca aagagcacca gtcggctcta gaagaagcac gtgcagatct ttttgccctg
     3481 tattacatga tggataataa acttattgaa cttggacttt tacctgaaaa agaggctgca
     3541 atggcagaat atgacagcta tatccgtaat ggaatgctta cacaacttgt cagaataaga
     3601 ccaggaaaac aaatcgaaca ggcacacatg agggcaagag caatgattgc ccgctgggca
     3661 tatgaaaatg gcaaagacga aaatgtgatt gaactattta cccgtgatgg caagactttt
     3721 gtgaaaataa acgattatga gaaactgaga aacctttttg gctcactgct gcgcgaggtg
     3781 caaaggataa aatcggaagg cgattacgaa gcagggaaaa cactaatcga gagttatgga
     3841 gtaaaagttg atcctgtact tcataatgaa atgcttgctc gttatgaaaa actgaagctg
     3901 gcaccctaca caggtttcct taatccgcga cttgtccctg tgtataacga caagggtgag
     3961 atcacggatg taaaggttga atatgaacag gattatttgt cgcagatgat ttattacggt
     4021 acaaattatt ctttcctgcc agcaatactt aattaattca ggtgaaatta gtgtgaataa
     4081 aaatcattct ttaaatgaaa gaaataaaac ttttagcttc agcaataatc cttgcggctg
     4141 cttcctcaac gatagcacag gtgcctgaag cagttttatg gaaaaagtat gagctgactt
     4201 tcccttcgac ggtaacttat gataatccgg tgcaggatat aaaaactttc caggtgacat
     4261 ttacctcacc tacaggaaga gtaaagacta ttaatggttt ctgggatggt gggagcacat
     4321 ggaaggcacg cttcatgccc gatgaaacag gtgattggaa atgggaaaca acatgctccg
     4381 atacaaagaa tgcaggatta cataatgtaa aaggggagtt ccgatgcata cccaataacg
     4441 gtaaatctga tcttgatatt catggcagaa taatccgtca acctggttta ttctatttga
     4501 cccatgctga tggtactccg tttctttata taggttgtac tgcctggaac ggagcactta
     4561 aatcgaccga tagtgaatgg gatcaatatc tgaagcatag ggctgaaaat caatatagtg
     4621 taatacagct tgttactacc caatggcgtg gatgtgacaa aaacagtctg ggtcaggtgg
     4681 catttgaggg tagtgccaga attcgcatta atccagaatt tttcaggcac ctcgataaaa
     4741 agattgacag ggtcaatgat tatggtcttg ttgcagcgcc agttattttg tgggcattgc
     4801 agactggagc aggaagggaa ctgagcccgg gttactacct gcctgaagat caggctatcc
     4861 tgctggcaaa gtatattgtt gcacgttacg gagcaaatca tgttatatgg ttacttgggg
     4921 gtgatggtat ttatacgggt atttatgagc aacgatggaa aacaatagga cgggcagtct
     4981 ttgatggaac tcatcagggg atagtagcac agcacccatg cggaagatca tggattggtg
     5041 agatttacag ggatgaagaa tggcttgata tagtgggtta tcaaagcagc catagcaacg
     5101 ataaaggaac agtggaatgg ataaca
//
LOCUS       DAOSNB010000188         5085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16588_length_5085_cov_4.632406, whole genome shotgun sequence.
ACCESSION   DAOSNB010000188 DAOSNB010000000
VERSION     DAOSNB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16588_length_5085_cov_4.632406"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2887
                     /locus_tag="PLG42_07940"
     CDS             <1..2887
                     /locus_tag="PLG42_07940"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU31178.1"
                     /translation="MLGRIATLFAGGDTSKVAVTMNALSAICSGFTILFLFWTITHLV
                     RKALCNDGIPASNQIFPVLASGVTGALAYTFSDTFWFSAVEGELYAMSSLITALVFWA
                     MLKWEDEADEPSAGRWIVLIAYLMGLGMGIHRLNLLVLPVLVFIYYFRKYEVTVKGLL
                     KTFVIAVALLWFVVFVFIPGVPRVGSWFELLFVNVFGLPYNSGLLFYVAFLIALLVIL
                     VRYSLKKQNILLNYIVTILTVILIGYSSYAMIIIRSSAKPPMNQNDPSNIFSFIYYIN
                     MEQYGSSPKVYGNYYNAPVTRVREVIAGYNKINGRYEPYYRPKYGYHEKFETIFPRMY
                     SSEPDHEAAYKYWGKIKGRRYTVESSSGKTTLVCPTFVENLRFFFRYQVGFMYFRYFM
                     WNFAGRQNDIQGNGNPLYGNWISGIKFIDNARLGDQDKIPEELRKNPGRNTYYCLPLL
                     LGLAGMLWQYKRDKYRFWVLFGFFFMTGLAIIVYLNQYPNQPRERDYAYSGSFYAFSV
                     WIGAGFMFFYEFIKKLVPEKPAAILTFVLLLAAVPALMASQNWDDHDRSGRYTARAIG
                     ANYLKSVAPNAILFTYGDNDSFPLWYAQDVEGTRTDVRVANLSYIQAGWYIEMMRQKA
                     WDSDPLPFSLSQDKYIEGKRTQLPVEDRVGRPVPLKQVVNFAGLDDRQAKLDITGRGD
                     FLNYLPASKFIIDVDTAFVLANGTVKKYFADSIVSPMIWEYTETDAFKGDLAIMDILA
                     TNNWVRPIYFSTTVPSSQYKGLEKFFVQEGLAYRVVPVRTGKPLPGEFGMIDTHVMYD
                     NLMNKFQWGNADAPGVYLDENNRRMFSNFKRMFGSLGIRLIETGDTLRALEVAHRGLS
                     LVPEEKIPHDYFAVTLAEVLIRGREKEEGLRLINKILDYARSYLQYIVRIPKEKRFGL
                     EYPIGVNMQAMIDIYRMYNRLGMLEEAKGLENELGIYYQELYPATSQ"
     gene            2892..3509
                     /locus_tag="PLG42_07945"
     CDS             2892..3509
                     /locus_tag="PLG42_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU31179.1"
                     /translation="MAVVYRLPWVAKWLYPQAMSTVKFSEQVLCLTFDDGPDPGSTPY
                     IMDILNRNAIKACFFCTGENAEKFPELVSMLVKEGHVSGNHTYSHLDGIGVTTKRYLD
                     DILRAVPYTSSVIFRPPFGRMKPGQYRALAGCYKIVLWDLLAADFAENVSGKKIIEEL
                     KVKIRSGSIIALHDKPISRKKNFLEEFIKMMKDSGYTFVLPDFNM"
     gene            3566..4837
                     /gene="purD"
                     /locus_tag="PLG42_07950"
     CDS             3566..4837
                     /gene="purD"
                     /locus_tag="PLG42_07950"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOU31180.1"
                     /translation="MKILILGSGGREHAIAWKLAQSSKVEEIFIAPGNAGTSMCGTNI
                     NISPENFSEVKEAVLSTGVGMVVVGPEAPLVGGIHDFFLQDDELKNIPVIGPVGAAAK
                     LEGSKDFAKGFMKKYGIPTAPYRTFDINNVGEARSFLESLKPPYVLKADGLAAGKGVV
                     ILNTVNEALSELDAILTGKFGEAGKKVVIEQYLRGIELSVFIVTDGKNYRILPEAKDY
                     KRAGEGDTGPNTGGMGAVSPVPFATEHFMYRVEKRIIKPTIEGLANEGIRYKGFIFFG
                     LMNVNGEPYVIEYNVRLGDPEAEVILPRISSDFYDLLEGVVNGNLNRRKIQIDSRYVA
                     TVVLVSGGYPGKYEKGKVIYGLDLVSDCIVFHSGTKIENGNIVTNGGRVLAITAYGLT
                     LYEALQNAYRNASLISWEGMYYRTEIGFDLL"
     gene            4839..>5085
                     /locus_tag="PLG42_07955"
     CDS             4839..>5085
                     /locus_tag="PLG42_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31181.1"
                     /translation="MLKAIKGVTPGVILLNVFILLALWFSAFLFQGQQSVFSDHRSMP
                     LYFILRKLTGHGQIEIIISLAFILLISYLLVHYNTILF"
BASE COUNT         1483 a          878 c         1196 g         1528 t
ORIGIN      
        1 tatgcttggg cgtattgcta cgttgtttgc aggaggagat acatcaaaag tggcagttac
       61 aatgaatgcc ctttcagcga tatgcagtgg atttacgatt ttgttcctct tctggactat
      121 aactcatctt gttagaaagg ctctttgtaa tgatggtatt cctgcttcaa atcagatttt
      181 tccggtcctt gcaagtggtg taacaggagc tctggcatat actttttctg acactttctg
      241 gttttcagct gtcgaaggtg agttatatgc aatgtcgtct cttataaccg ctcttgtatt
      301 ctgggcgatg ctaaaatggg aagatgaagc tgatgaaccc tctgctggac ggtggatcgt
      361 tctgatagca tacctgatgg gactgggcat gggtattcac cggcttaatc tgcttgtgct
      421 tcctgttctg gtgttcattt attatttcag gaaatatgaa gtgactgtaa aagggttact
      481 taaaacattt gttatagcag tagctcttct atggtttgta gttttcgttt ttattcctgg
      541 agttccaagg gtgggaagct ggtttgaact tctatttgtt aatgtattcg gtctgcctta
      601 taattcagga cttttatttt atgtggcatt tctcattgca cttttagtaa tacttgtaag
      661 atattctcta aagaagcaaa acattctcct taactatatt gttaccatat tgacggttat
      721 tcttataggc tattcatcat atgccatgat aattataagg tcatcggcaa agccgcctat
      781 gaaccagaat gacccgtcaa acattttttc gtttatctat tatataaaca tggagcagta
      841 tggcagttca ccaaaagttt atggtaatta ttacaatgct ccggtaacga gagtcaggga
      901 agttattgct ggatacaaca agataaacgg aagatatgaa ccttattaca ggccgaaata
      961 cggataccat gagaaattcg aaacaatttt ccctcgcatg tacagttcag aacctgacca
     1021 tgaagctgca tacaaatact gggggaaaat aaagggcagg cgttatactg ttgaatcttc
     1081 atcaggtaaa acgactcttg tttgtcctac ttttgtggag aatctgagat tttttttcag
     1141 gtaccaggta gggtttatgt atttcaggta ttttatgtgg aattttgcgg gcaggcagaa
     1201 tgatattcaa gggaatggaa atcctcttta cggcaactgg ataagcggga taaaatttat
     1261 agataacgca aggcttggcg atcaggataa aataccggag gaattaagga aaaatccagg
     1321 gcggaacaca tattactgtt tgcctctgct cctgggcctg gcaggaatgc tctggcaata
     1381 caaaagggac aagtacaggt tctgggtgct ctttggcttc tttttcatga cagggctggc
     1441 aattattgta tatctgaatc aatatccaaa tcaacctcgc gaaagggatt atgcctattc
     1501 aggttcgttt tatgcatttt ctgtctggat tggagcaggc tttatgtttt tttatgagtt
     1561 tattaaaaag cttgttcctg agaaacctgc agctattctc acatttgtat tgttgctggc
     1621 tgcagttccg gctcttatgg catcacagaa ctgggatgat catgacaggt cgggccgcta
     1681 cacagcacgg gctataggtg caaactatct taagtctgtt gcaccgaatg caattctgtt
     1741 tacttatgga gataatgatt catttcctct ctggtatgct caggacgttg aaggaaccag
     1801 aacagatgta agggtagcaa atctgagtta cattcaggct gggtggtata ttgaaatgat
     1861 gaggcagaag gcatgggatt ctgatcctct gcccttctca ctcagtcagg ataaatatat
     1921 tgaaggcaag agaactcagt tgccagttga agaccgtgtt gggaggcctg tgccgcttaa
     1981 acaggtggtg aattttgccg gacttgacga ccggcaggca aaattagata ttacaggaag
     2041 aggagatttt ctcaattacc tgccggcaag taaatttata attgatgttg acactgcgtt
     2101 tgtacttgca aacggaacag taaagaaata ttttgccgac agcatagtat caccgatgat
     2161 ttgggagtat accgaaacag atgcatttaa gggggacctt gcaattatgg atatactcgc
     2221 gacgaacaac tgggttcgcc caatctattt ttcaaccaca gtaccttcct ctcagtacaa
     2281 ggggcttgag aagttttttg tgcaggaggg gcttgcctac agggtggtgc ctgtacgaac
     2341 ggggaagcca ctacctggag agttcggaat gattgacaca catgttatgt acgacaacct
     2401 gatgaataaa tttcaatggg gcaatgcaga tgcacccggt gtttaccttg atgagaacaa
     2461 cagaaggatg ttcagcaatt tcaaaaggat gttcggctcc cttggcatca ggcttattga
     2521 gacaggagac acgctcagag ctctcgaagt tgctcacagg ggactctctc tggtgcctga
     2581 agagaaaatt ccgcatgact atttcgcggt tacacttgca gaggttttaa taagagggcg
     2641 tgaaaaggaa gaaggtttaa ggctaataaa taaaatactg gattatgcac gtagttacct
     2701 tcaatatatt gtaagaatac ctaaggaaaa gagatttgga cttgagtatc ctataggggt
     2761 aaatatgcag gcaatgattg acatttacag aatgtataac cgtttgggaa tgctggaaga
     2821 ggcaaaggga cttgagaatg agcttggaat ttattatcag gagctttatc cggcaacatc
     2881 acaatgaaaa tatggcggtg gtttacagat tgccatgggt tgcgaaatgg ctttatcccc
     2941 aagcaatgtc gaccgttaag tttagcgagc aggttttatg cctgactttt gacgacggac
     3001 ccgacccagg ctcaactcct tatataatgg atattcttaa caggaatgct ataaaagctt
     3061 gtttcttctg cacaggagaa aacgctgaaa aattccctga gctagtttca atgctggtta
     3121 aggagggaca tgtatcaggg aatcatactt acagtcattt agatgggatt ggagtaacaa
     3181 caaagaggta tcttgatgac attcttagag cggttcctta cacctccagt gttattttca
     3241 gacctccttt tggccgcatg aaaccagggc aatacagagc tctggcaggt tgttataaaa
     3301 tagttttatg ggatcttctg gcagcagact ttgcagaaaa tgtttcgggc aaaaagatta
     3361 ttgaggaact gaaagttaaa ataagatccg ggtcaataat agcactgcat gataaaccta
     3421 tcagccgaaa gaagaatttt cttgaagaat ttataaagat gatgaaggat tctggatata
     3481 cttttgtatt acctgacttt aatatgtaaa ctttgaattt tatatccgtc agaaatacta
     3541 tttttgaaga aatttgtaag aagaaatgaa gatacttatt ctgggatcag gaggacgaga
     3601 gcatgctatt gcatggaaac ttgctcagag cagcaaagtg gaagagattt ttattgctcc
     3661 cggcaatgca ggtacttcaa tgtgtggaac gaacataaac atttcgccag aaaatttttc
     3721 tgaggtaaag gaagcggtcc tttcgacagg ggttggcatg gtagtagtag gacctgaggc
     3781 acctcttgta ggaggtatac atgatttttt ccttcaggat gatgagctta aaaatatacc
     3841 tgtgataggt cctgttggtg ctgctgcaaa acttgagggg agcaaagatt ttgcaaaggg
     3901 atttatgaaa aaatatggca taccaacagc tccataccgc acatttgaca ttaataatgt
     3961 aggagaggcc cgttcatttc tcgaatctct caagccaccc tatgtgctta aggctgatgg
     4021 acttgctgca gggaaggggg ttgtaattct gaatactgtt aacgaagcac tttcagagct
     4081 ggatgcaata ctaacaggaa aatttggaga ggcaggaaag aaagttgtaa tagagcaata
     4141 tctgagggga atagagttat cagtttttat tgtaacagat ggtaagaatt accgtatact
     4201 tcctgaggca aaggattaca agagggcagg agagggtgat acgggtccga atactggagg
     4261 aatgggtgca gtttcaccgg tgccttttgc tactgaacat tttatgtaca gagttgaaaa
     4321 aaggattatt aaacccacca ttgaaggcct tgcaaatgag ggtatcagat acaaaggatt
     4381 cattttcttt ggcttaatga atgttaatgg agaaccttat gtaattgaat ataatgtacg
     4441 cctgggtgat cctgaagctg aagttatact tccgagaatc agttccgact tttatgacct
     4501 tcttgaagga gtagtaaatg gtaatcttaa tcgccggaaa atacagatag acagcaggta
     4561 tgttgcaaca gtagtgttgg tgtcaggagg atatccggga aaatacgaga aaggaaaggt
     4621 aatttacggg ctcgatcttg tttcagattg tattgtattt cattcgggaa cgaaaataga
     4681 aaacggtaac attgtaacga acggcggaag agtgctggct ataacagcct atggtttaac
     4741 attatatgaa gcactacaga atgcttacag aaatgcctca cttatttcct gggaaggaat
     4801 gtattaccgt actgaaatag gttttgatct gttatgagat gcttaaagca attaaaggtg
     4861 taacacccgg cgtaattctt ttaaatgttt ttattctgct ggcactctgg ttcagtgctt
     4921 ttctttttca agggcaacaa tcggtttttt ccgatcacag atccatgcct ctctatttca
     4981 ttctcaggaa gttaactggc catggtcaaa tagaaataat aataagtttg gcctttattc
     5041 ttctaatttc ctatctgctt gttcattaca acacgattct atttt
//
LOCUS       DAOSNB010000189         5058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16689_length_5058_cov_3.902459, whole genome shotgun sequence.
ACCESSION   DAOSNB010000189 DAOSNB010000000
VERSION     DAOSNB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16689_length_5058_cov_3.902459"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="pfkA"
                     /locus_tag="PLG42_07960"
     CDS             complement(<1..908)
                     /gene="pfkA"
                     /locus_tag="PLG42_07960"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOU31182.1"
                     /translation="MGKIKKIGVLTSGGDAPGMNAAIRAVVRTAIYHGLEVMGIRQGY
                     AGLINADFIPLVSSSVSDIIQRGGTILKTARCEEFRTPEGRQKAYENLKAAQIDGVVV
                     IGGDGSFTGARLFNEEYDIPFVGVPGTIDNDIYGTDYTIGYDTALNTVVQAVDKIRDT
                     ASAHDRMFFIEVMGAEAGFIALFSGIATGAEAIIIPEVKGEERDLKKILFDGNKRKKS
                     SNLIIVAEGDEEGGAFAWAEKFKNEYPDINIRVTVLGHLQRGGSPTAIDRVNASRLGS
                     AAVEALLDDQKSVMVGLQNDEIVLVP"
     gene            complement(974..1855)
                     /locus_tag="PLG42_07965"
     CDS             complement(974..1855)
                     /locus_tag="PLG42_07965"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOU31183.1"
                     /translation="MVIEIDENSGFCFGVRNAVETAEKALMAGEKIFSLGQIVHNDIE
                     VERLSKMGLKTIDYDEFRRLRDCKVLIRAHGEPPETYRIAKENGITIIDATCPIVKRL
                     QQKIRQTWEEIKIKGGQVVIYGKPGHAEVLGLIGQTDNNAILTDGEINLDRIDFRKEI
                     HLFSQTTMNPEAYEALAEKMRKKIKEYGAPDPGKLLTVHRTICTQVSHRQPHLESFAR
                     SHDIIIFVSGKESSNGKMLFSICKNVNPESYFVSSPGDIKKEWFIGKQKAGICGATST
                     PGWLIRKVSEVISSFNI"
     gene            complement(1836..2540)
                     /gene="cmk"
                     /locus_tag="PLG42_07970"
     CDS             complement(1836..2540)
                     /gene="cmk"
                     /locus_tag="PLG42_07970"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOU31184.1"
                     /translation="MKKKLIIAIDGYSSCGKSTFAKAIAKELNYIYIDSGAMYRAVTL
                     YCLRKGFITKNGIDTEGIKNSLKDIHITFVYNPDKGEYETWLNSENVENEIRSIEVSN
                     FVSRISQIPEVRTRMVELQRQIGVFKGIVMDGRDIGTVVFPDADIKIFMTASVDTRAQ
                     RRFDELQAKGQPVDYREIKKNIIERDVADENREISPLRKAKDAIILDNTNMTVEEQME
                     WIRGIIQEKLHGDRDR"
     gene            complement(2726..4381)
                     /locus_tag="PLG42_07975"
     CDS             complement(2726..4381)
                     /locus_tag="PLG42_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31185.1"
                     /translation="MKDIFFLILFACLFSIAYGQSRTGSEDVVIINGKGKIRVSTGEK
                     GELIINISPKDSKRLQSKGVVRYSDFGARGDGKTDDIDAIAAAHAFANLYGLKVKADD
                     KATYYIGGKNRTAIIQTDTDFGNAAFIIDDSEVQNRNANIFLVSSTLKPFKPEGITSL
                     KKNQPKVDIRLPGPCLIAVTDSNVRRYIRFGPNQNNGSPQTDIFIVDAEGNVDMNAPI
                     IWDFDHVTAITALPIDEITLTLRGGRFTTIANSAESKYNYYSRGIAIRRSHVVVDGLQ
                     HFITGEGDHGAPYGGFINIGDCAFVTVQNCVFTGHKVYETIGSAGVSVSMGTYDISLN
                     SAINVSFINCTQTNDIKDSRFWGLMGSNYCKNLILDNCIFSRFDAHMGVANATIRNST
                     LGHQGINAIGTGTFLVENSTIYGRNLINLRSDYGSTWQGEFIIRNCTFVPAGGRPVTA
                     SLIGGSYTGQHDFGYVCYMPERIIIENLKIDDSNHPDNYPGPAIFANFNPKMTDDTYQ
                     EKFPYVITREVILRNVTTASGKPLRISDNQYMFRNVKVVTENL"
     gene            complement(4443..>5058)
                     /locus_tag="PLG42_07980"
     CDS             complement(4443..>5058)
                     /locus_tag="PLG42_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31186.1"
                     /translation="LWSKIFDTVFSVGVCLKPVLSHLEKYSSFGIALDIGAAWHSKNN
                     LTSAGIVVKNAGFQVTTYTGETHEKLPFEIIAGISHRLAHGPFRFSLTLRHLEKFDIT
                     NSYGSDNTGTYEKAVFSEKLLRHVIAGAEFIPHKNFYFSLGYNYQRRKELQVESKVST
                     VGFSWGFGFNTSFLSFGFGRATYHLAGASNHFTLSLRPGRLIKR"
BASE COUNT         1318 a         1147 c          977 g         1616 t
ORIGIN      
        1 aagggcacaa ggactatttc gtcgttctgc aaaccaacca tcacactttt ttgatcatcg
       61 agcagggctt cgacagctgc gcttccgagc ctgctggcat taaccctgtc gatggcagtg
      121 ggtgagcccc cgcgctgaag gtgtccaaga acagttactc ttatattaat atcagggtat
      181 tcgtttttaa atttttcagc ccaggcaaaa gcacctccct cttcatcgcc ttcggcaaca
      241 attatcagat tgctcgattt ctttctttta tttccatcga ataatatttt cttaaggtcg
      301 cgttcttccc ctttaacttc cggaattatg attgcttcag cacctgttgc tattccgctg
      361 aaaagagcga taaagcctgc ctcagcgccc atcacctcta taaaaaacat tctgtcgtgt
      421 gcactggctg tatcccttat tttatcaact gcctggacaa cagtatttaa agcagtgtca
      481 tagccaatcg tgtaatcagt tccgtaaatg tcgttatcaa ttgttccagg cacacctacg
      541 aaggggatat catattcttc attaaatagc cttgcaccag taaaggagcc gtctccacct
      601 atcaccacca caccatctat ttgtgctgct ttcagattct cataggcttt ctgtcttcct
      661 tccggcgttc ggaattcttc acaccttgcc gttttcagta tcgtgccacc tctctgtata
      721 atatcgctta cactgctcga tacaagcgga atgaaatccg cgttaatcag gcctgcatag
      781 ccctgcctga tgcccataac ttcaagccca tgatatattg cagttcttac aaccgctctt
      841 attgcagcat tcatacctgg agcatctcca cccgatgtta aaactcctat cttctttatt
      901 tttcccatct ttaattgatt ataattcagc ccgcaaaggt agtgtttttc tgcttagatt
      961 gttaattggg agattaaata ttaaatgaag aaattacttc acttactttt ctgataagcc
     1021 atcctggagt ggatgttgct ccgcatattc ctgctttctg ttttccgatg aaccattctt
     1081 ttttgatatc tccaggtgac gaaacgaaat atgattcggg attaacattt ttacagattg
     1141 aaaatagcat tttaccgtta gagctttctt tgccgcttac aaatattatt atgtcgtgtg
     1201 aacgcgcaaa tgattccaga tgtggctggc gatgtgagac ctgagtgcag attgtccggt
     1261 gcacggtcag cagtttcccg gggtcgggtg cgccatactc tttgattttc ttcctcattt
     1321 tttcagccag agcctcgtat gcttctggat tcatggttgt ctgggaaaaa agatgtatct
     1381 cctttctgaa atctatcctg tcgaggttta tctctccatc agttagtata gcattattgt
     1441 cggtttgtcc tataaggcca agcacttcgg catgccctgg cttgccgtag ataacgactt
     1501 gtccaccctt tatttttatt tcttcccatg tttgtctgat cttttgctga agtcttttca
     1561 caatggggca ggtagcatca ataatggtga tgccgttttc ctttgctatt ctgtaagttt
     1621 cgggcggttc tccgtgggct cttatcagca ccttgcaatc tcgaagtctc cggaactcat
     1681 cataatcaat agttttaaga cccattttgc tgagccgctc aacttctata tcattatgca
     1741 caatctgtcc gagggaaaaa attttttcgc cagccattaa tgccttttct gctgtctcaa
     1801 cagcatttct tacaccgaaa cagaatcctg aattttcatc tatctctatc accatgcaat
     1861 ttctcctgaa ttatacctct gatccattcc atctgttctt caacggtcat atttgtatta
     1921 tcaagtatga tggcatcttt tgcttttcgc agtggactga tttcacggtt ttcatcagcg
     1981 acatcccgtt caatgatatt ctttttgatt tccctgtagt cgacaggctg tccttttgcc
     2041 tgcagctcgt caaagcgcct ctgcgccctt gtatcaaccg atgctgtcat gaatattttt
     2101 atatcggcat ccggaaaaac aacagttcct atatcacgac cgtccatcac tataccctta
     2161 aaaacaccaa tctgcctctg aagttcaacc atccttgtcc ttacttcggg tatctggctt
     2221 atcctgctta cgaagtttga cacctcaata cttctgattt cattttcaac attttcagaa
     2281 tttaaccatg tttcatattc acctttatca gggttgtaca cgaaggttat gtgaatatcc
     2341 ttaagtgagt tttttattcc ttcggtgtcg ataccgtttt tcgtaataaa ccctttcctc
     2401 aggcagtaga gagtgacagc cctgtacatg gctccgctgt cgatgtatat gtaattcaat
     2461 tctttagcta tagcctttgc aaatgtgctt ttaccgcatg aagagtaacc atcaattgct
     2521 atgataagtt tctttttcat tttaactata aaaataccgg atctggattt tatctcttta
     2581 tagaattaag tataccatat ccgggatgtt tttaattttt ttggtaaatt taaccgaaga
     2641 tataaaaaac ctgaaaagaa attattgaca gatgtcagaa ttcattttgt attgttcata
     2701 acagatactg gttgctctgg gttagctata aattttcagt caccaccttt acattcctga
     2761 acatatactg attgtcgctt attcgcaggg gtttgccact ggctgtggta acattcctga
     2821 gaatcacttc cctggttatt acataaggaa atttttcctg gtaggtatca tctgtcattt
     2881 tcggattgaa gtttgcaaat atagccggac ccggataatt atcaggatga ttggaatcgt
     2941 ctattttaag attctcgata attatccttt caggcatata acagacatat ccgaagtcgt
     3001 gttgtccggt atacgagcct ccaatcaggc tggcagttac aggtcttcct ccggctggca
     3061 cgaaggtgca attgcgtatg atgaactcgc cctgccatgt gcttccgtaa tcggaacgga
     3121 gattgattaa attcctgcca tatatggtgg agttctcaac taaaaaggta cccgtaccta
     3181 tggcatttat accctgatgc cccagtgttg aattgcggat agtggcattg gcaaccccca
     3241 tatgggcatc gaaacgcgaa aaaatacagt tgtcaagaat gagattctta cagtaattgg
     3301 agcccattaa accccagaat ctgctgtctt tgatatcatt tgtctgggtg cagtttataa
     3361 atgacacatt aattgcactg ttaagtgaaa tgtcgtatgt ccccattgag acactgacac
     3421 ctgccgaacc aattgtttca tagactttat gtccggtaaa gacacaattt tgtactgtaa
     3481 caaaggcgca gtcgcctata ttgataaacc caccgtacgg tgcgccatga tcgccttcgc
     3541 cggtaataaa gtgctgcagg ccgtcaacaa caacatgtga acgccttatt gctataccac
     3601 ggctgtaata attgtatttc gattctgcac tgttggctat ggtggtgaaa cggccacccc
     3661 ttaaggtcag ggtaatctca tcaataggaa gtgcagttat agctgtaaca tggtcgaaat
     3721 cccatattat tggggcattc atatccacat taccctccgc gtcaacaata aaaatatccg
     3781 tttgagggga gccgttgttt tggttgggac caaaccgtat atagcgcctg acgtttgaat
     3841 cggtgacggc aataaggcat ggcccgggca gccggatatc aaccttcggc tgattctttt
     3901 ttagggaagt gataccctca ggcttaaatg gtttcagagt ggaactgacc agaaatatat
     3961 ttgcattgcg gttctgtact tcagagtcgt caattataaa ggcagcattg ccaaaatcag
     4021 tgtctgtctg tataatagca gtacggtttt tgcctccgat ataataggtc gctttatcgt
     4081 cagcttttac tttcaaccca tacagatttg caaacgcatg agcagccgca atagcatcga
     4141 tatcatcggt tttgccgtcg cctctggcac cgaaatcgct gtatcgaaca acccccttcg
     4201 attgtaaccg ttttgaatct tttggtgata tgtttataat caactctcct ttttcaccag
     4261 tacttacccg aattttacct ttcccgttta ttatcacaac atcttcagat ccggtccttg
     4321 attgtccata ggctatgcta aacagacagg caaacaaaat gagaaaaaag atatctttca
     4381 tagaactcta tttttttaat taccggaaat caaaaataac tgattgcatc aaataattaa
     4441 tatcatcttt tgatgagtcg gccgggccgg agtgaaaggg taaaatgatt tgaggcaccg
     4501 gcaaggtgat atgttgccct cccgaagcca aagctcagaa atgaggtatt gaagccaaaa
     4561 ccccatgaaa aacctactgt tgaaactttc gattcgacct gaagttcttt tcttcgctgg
     4621 taattgtatc ccagagaaaa ataaaagttt ttgtgcggta taaactcggc cccggcaatg
     4681 acatgcctta atagcttttc tgaaaaaact gctttttcat aagtgccggt gttatcgctc
     4741 ccgtatgaat ttgtaatatc aaatttttcg aggtgccgga gtgtgagaga aaacctgaag
     4801 ggaccgtggg caagtcggtg cgagatccct gcaattattt caaacggaag tttttcgtgt
     4861 gtttcacctg tataagtagt gacctggaag ccagcatttt ttacaacaat ccctgccgag
     4921 gtaagattgt tcttcgaatg ccatgcggca cctatatcaa gggcaatacc aaaagaagaa
     4981 tatttttcaa ggtgggacag aaccggcttc aaacagacac ctactgagaa aacggtatcg
     5041 aatatcttcg accataga
//
LOCUS       DAOSNB010000190         5048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16735_length_5048_cov_4.420188, whole genome shotgun sequence.
ACCESSION   DAOSNB010000190 DAOSNB010000000
VERSION     DAOSNB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16735_length_5048_cov_4.420188"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="PLG42_07985"
     CDS             complement(<1..1847)
                     /locus_tag="PLG42_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU31187.1"
                     /translation="MNKIHTFTTIGLLISSLTLYSQANKKTEQVRDTLSPVTGEKVIP
                     LSEIVVSSFRVNHAVKELPAPLAVVGSYNFRKFSSLTLSNVLASEPGISMGSDGIWST
                     KAVIRGMNENRLVTLVDGNRIETATDLTASLSMIDINDIERVEVIKGAQSSLYGTGAM
                     GGIINIITKDGYFGNRPYVSGNITSGYASANNFFTGHGEVNTGSSRWYLRLSGTYGNA
                     DDIRTPEGILPNSQFAFNNLSAKIGFKVFRNHILRVQFQNYHATDVGIPGGEAFPGPA
                     EAKYTDISRQLISVDYEIKDITRTLTQLKINYFMQYIQRDVEMIPNTVTVTPLPSGGS
                     QRTTPELVVPIGNHLINGTQLQSTWKFGEKNMLIAGIDAWARKVTTERTKYIKTEVLD
                     ASGTVVKTNNIVRGETPIPVSTFGSAGIFLQDEHQYNERLRIIAGGRFDGIIVKNEKG
                     YDVDYLIVNGVRNDTPPNQRITFEKGQTGNISWSANAGILYRIINDADLSVNMARAFR
                     APSLEERFKYIDLGNFVRLGDPELLPERGLSSDLGIRVWKPDFNLQADVFINRISNMI
                     VETPGQFVYTINTGPAEGTTDTLPALVNANVRKAILYGFDFGLQYNFHS"
     gene            complement(1849..2094)
                     /locus_tag="PLG42_07990"
     CDS             complement(1849..2094)
                     /locus_tag="PLG42_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31188.1"
                     /translation="MEKRIGTAIILVENRKSVQRLNEILSNHSDIIIGRQGLNRSTGQ
                     SIISLVLEGTTDRISSLTGHLGRLKGIQVKSVLLKNK"
     gene            2359..3336
                     /locus_tag="PLG42_07995"
     CDS             2359..3336
                     /locus_tag="PLG42_07995"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU31189.1"
                     /translation="MVKKYLSLILLFSLVLFLLCECRSNVTRKDSSGVYTIATLKGPS
                     SMGMIKLIDSLNSGGPYNVKATILDEPMQVRKMMIDKTADFAILPTTMAAIIYNKGFG
                     YKLVAVPVWGTLYLVGSDSTVKRWDDLRGRRVHVMARGMTPDELFRYLLRKNGIDPEK
                     EITLDYSFPTHVDLANAVGAGRTELAVLSEPLASMVMKRNGSVRRIFSLNEEWAKFEG
                     IPIAETAFMAKEEMLKNNRDITEKLLSSYEASTNWVNLYPDSAATLIVKYGILPDYES
                     AFNAIPLSNLKFARAKDVKKEVNEYLSVFYRMNPDIVGGKVPDENFIYQ"
     gene            3314..4069
                     /locus_tag="PLG42_08000"
     CDS             3314..4069
                     /locus_tag="PLG42_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU31190.1"
                     /translation="MKISFTSRQYVSLVSVIIILVTWKFFSLYFNSEFVLPSPEKTLL
                     TTLKLFVDDRFLVTVGTTILRSLTGFIVSFFLGLLAGIAAGVNPYVDAFLRPVLVTIR
                     SVPIIAIILLALIWLTPGTVPVFIAMLTMFPFICTNVSDGIRSVDRDLVQMAKFYRVE
                     NARIVKELYIPAIMPFIISGASSAMGIGWRAIITGEVLSHPRYGIGTLMQNAQTFLNV
                     DVVIAWTIIAVVISYGFEKVIRWSERKLIIWRA"
     gene            4072..4782
                     /locus_tag="PLG42_08005"
     CDS             4072..4782
                     /locus_tag="PLG42_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOU31191.1"
                     /translation="MTLEVKGLHKRYGEVEIFRDFSISFTEGMITCILGPSGCGKTTL
                     LNILGKIIPPDGGTLAGFDGKIVSYIFQEPRLLPWKSVRGNIEFVLDRNLLPEERKKI
                     TDRFISLVELEDFADYYPAKLSGGMRQRVSIARAFAYPSDIILMDEPLKGLDIKLKLN
                     LIQTFSRIWQEDRRTVIFVTHDVDEALLLGNEIVVFSRAPVEIVTQKQIGVPIEERSL
                     NSDYLKNLEKELLRALGG"
     gene            4901..4972
                     /locus_tag="PLG42_08010"
     tRNA            4901..4972
                     /locus_tag="PLG42_08010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4934..4936,aa:Glu,seq:ctc)
BASE COUNT         1368 a          984 c         1135 g         1561 t
ORIGIN      
        1 tttgaatgga aattgtactg taaaccaaag tcgaagccat aaagtattgc ctttcttaca
       61 ttggcattga caagggcagg aagtgtatcg gttgtacctt cagcaggacc tgtattaatt
      121 gtatagacga attgcccggg cgtctcaaca atcatgtttg atatacggtt tatgaaaaca
      181 tctgcctgga ggttgaaatc gggtttccat acccttatac cgagatctga agaaagtcct
      241 ctttcaggca aaagttctgg atctcccagt ctgacaaaat tcccaaggtc aatatattta
      301 aaccgctctt caagcgatgg tgctctgaac gccctggcca tattgactga aagatcggca
      361 tcattaataa ttctgtagag gattccggca tttgcactcc atgagatgtt gcctgtttgt
      421 cccttttcga aagttattct ctggttgggt ggggtgtcgt tgcgtactcc gttaacaatc
      481 aaatagtcaa cgtcataacc cttttcattc tttacaatta tgccgtcgaa acgacctcct
      541 gcgataattc tcaaccgttc gttatactga tgttcgtcct gaaggaaaat acctgcgctt
      601 ccaaaagttg aaacagggat tggggtctct ccccttacaa tattgttggt tttaacaact
      661 gttccactgg catccaatac ttctgttttt atatattttg tccgttcggt ggtaacttta
      721 cgtgcccatg catcaattcc tgcaatgagc atattttttt caccaaattt ccatgtactc
      781 tgaagctggg ttccgtttat aagatggttc ccgattggca cgacgagttc cggggttgtt
      841 ctctgggaac cacctgatgg aagaggcgtt acggtaacag tgttgggtat catttcaaca
      901 tcgcgctgaa tgtattgcat gaaataattt atcttcagct gggtaagagt acgggtgata
      961 tcttttatct cataatctac tgaaatcagc tgccggctga tatcggtata ttttgcttct
     1021 gctggtccag ggaaagcttc accccccgga atacccacat cagttgcatg atagttctga
     1081 aactggaccc tgaggatatg gttacggaat accttaaaac cgatttttgc agacagatta
     1141 ttaaaagcga actggctgtt tggcagaata ccttcaggag tgcgtatatc atcagcatta
     1201 ccgtatgtcc cgctcagccg gagataccat ctcgaagaac cggtattcac ttcaccatgg
     1261 ccggtaaaga agttattggc agaggcatat cccgaagtga tattgcccga tacataaggt
     1321 ctgtttccaa aatagccgtc tttagttata atgtttataa ttccgcccat ggcacctgta
     1381 ccataaagtg acgactgtgc accttttatt acttcaacgc gctctatatc gtttatgtct
     1441 atcatgctga gtgaggctgt caggtcggtg gctgtttcaa tcctgttacc gtcgaccagg
     1501 gtaacaaggc gattctcatt catgccacgt atgactgctt ttgttgacca tatgccatcg
     1561 ctccccattg aaataccggg ttcggaggca agaacattcg aaagtgtcag tgaagaaaac
     1621 ttcctgaaat tgtaagagcc aacaacagcc agaggtgccg gtaattcttt tactgcatgg
     1681 ttaaccctaa aactggacac cacgatttca cttaagggaa ttactttttc acctgtaacg
     1741 gggcttagag tatcccgcac ctgttcagtc tttttgtttg cctgactata aagagtgagg
     1801 gaagaaatca ataaaccaat tgttgtaaaa gtatggattt tgttcatatc acttattttt
     1861 aaggagtacc gattttacct ggataccctt cagccggccc agatggccag taagggaact
     1921 gatccggtcg gtagttcctt caagaacaag agagatgata ctttggcctg tactccggtt
     1981 aagtccttgc cgtcctatta taatgtcgga atggtttgaa aggatttcgt tgagtcgctg
     2041 aacgcttttg cggttttcta ccagaattat tgctgttcct attctctttt ccatcagaat
     2101 tacctccgga tcagattttt gttttgtttg aacctgtagc tccttgagcg gaataaatcc
     2161 cgcttcgcgg attagtaaca ggtgttaact tttgcgatgc aaatatagaa ttatttttta
     2221 tttgctaaac ctttttcaga tttattataa caataaattt tggcggtgtt acaggcatgg
     2281 actgattgta aaagtaactt ttgaagttga attaagtatc aaaaaataaa tagctttgct
     2341 ctcggaattt taagtagtat ggtaaaaaaa tatctttctt taatactgtt gttttcttta
     2401 gttctatttc ttctgtgcga gtgcaggagt aatgtaaccc gtaaagattc atcaggagtt
     2461 tatactattg ccactcttaa aggtccatcg tcaatgggaa tgataaagtt gattgacagc
     2521 ttgaattctg gggggccata taacgtgaaa gcaactatcc ttgatgaacc catgcaggta
     2581 cggaaaatga tgattgataa aactgctgat tttgccatcc tgcctactac catggccgca
     2641 attatttata ataaaggttt tggttacaaa cttgttgccg ttcctgtatg gggtacgctc
     2701 tatcttgttg gaagcgacag cacggttaaa agatgggacg acctccgtgg cagaagggtt
     2761 catgtcatgg cacgcggtat gactccagat gaacttttca gatatctcct cagaaaaaat
     2821 ggcattgatc ctgaaaaaga gataacactc gattatagct ttccgacaca tgttgacctc
     2881 gcaaatgctg ttggtgccgg caggactgag ctggcggtcc tttccgaacc acttgcaagc
     2941 atggttatga aaagaaacgg atctgtaaga agaatttttt cgctcaatga ggagtgggct
     3001 aaatttgaag gaattccaat tgctgaaaca gcctttatgg caaaggagga gatgctcaaa
     3061 aacaacaggg atattacaga aaagcttctg tcgtcgtatg aggcttcaac aaactgggta
     3121 aacctttatc ccgacagtgc agcaactttg attgtcaaat atggcattct gcccgattac
     3181 gagtcggcat tcaatgcgat tccgctttca aatcttaaat ttgcaagggc aaaagatgta
     3241 aaaaaagaag ttaacgaata tcttagtgtg ttttaccgga tgaatccgga tatagttgga
     3301 ggtaaagtac cggatgaaaa tttcatttac cagtaggcaa tatgtgagtc ttgtttcggt
     3361 aatcattatt ctggttacct ggaagttttt ttcactttat ttcaattcag aatttgttct
     3421 tccttcaccg gaaaaaacgc tgttaaccac tttgaagctc tttgttgacg acaggttttt
     3481 agtgacagta ggcactacca ttcttcgcag tcttacggga ttcattgttt ctttctttct
     3541 gggtttgctg gctggtatag ctgccggtgt caatccttac gttgatgcat ttctcagacc
     3601 tgtgctggtt accatcaggt cggtaccgat tattgcaatt atccttcttg cccttatttg
     3661 gctgacacct ggaacagttc cggtatttat agcaatgctt accatgtttc cgtttatctg
     3721 tacaaacgtt tctgatggta tcagaagcgt tgaccgggac ctggtgcaga tggcaaagtt
     3781 ttatagagtt gagaacgcca gaattgtgaa ggaactttac atacctgcaa taatgccgtt
     3841 tattatcagc ggagcctcga gtgctatggg aataggctgg agggcaataa taactggcga
     3901 ggtgcttagt catcccagat atggcatcgg cacgcttatg caaaatgcac agacttttct
     3961 taatgttgat gttgtgattg cctggactat cattgcagtg gttatcagct atggttttga
     4021 gaaggttatc cgctggagtg agagaaaact tattatctgg agggcataag tatgaccctt
     4081 gaggtaaaag gtctgcataa aagatatggt gaagttgaaa ttttcaggga tttcagcatc
     4141 agttttacgg agggtatgat aacatgtatc cttggtcctt cgggatgtgg caagacaacc
     4201 ttgctgaata ttcttggtaa aataatccca ccggatggcg ggacacttgc aggttttgac
     4261 gggaagattg tttcatatat ttttcaggag cctcgtttac ttccctggaa gagtgtgagg
     4321 ggaaacattg aatttgttct tgacagaaac ctattaccgg aagagaggaa aaagataacc
     4381 gacaggttta tcagtctggt tgagcttgag gattttgcag attattaccc ggcgaaactg
     4441 agcggaggta tgcgtcagag ggtttcaata gcaagagctt ttgcctatcc ttcagacatt
     4501 atcttaatgg atgaaccgct taaagggttg gatatcaagc ttaagttgaa tctgattcaa
     4561 accttttcaa ggatatggca ggaagacagg cggactgtta tttttgtaac acacgatgta
     4621 gatgaggctt tgctgctggg caatgaaata gttgttttca gcagggcgcc tgttgagatt
     4681 gttacccaga agcaaatagg agtacctatt gaagagcgct cactgaattc tgattacctg
     4741 aaaaacctgg agaaagagtt actcagggca cttggaggct gagggctgga ttaaaaagta
     4801 cacacattcc atttattgga ttattaatta aaatatttat ttttgcagcc tgttgaaaag
     4861 gccggcaata gattgaaaaa attcgcaacc ggtcagtaac ggcccattcg tctagtggtt
     4921 aggacagcag attctcagtc tgtaaacgac ggttcgattc cgtcatgggc tactaggaat
     4981 cagtgctttt ctttctcagt cagatattca attgcaaact tcagagttgt atcaagtttt
     5041 tcccagat
//
LOCUS       DAOSNB010000191         5042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16757_length_5042_cov_4.786645, whole genome shotgun sequence.
ACCESSION   DAOSNB010000191 DAOSNB010000000
VERSION     DAOSNB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16757_length_5042_cov_4.786645"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..669)
                     /gene="rpsD"
                     /locus_tag="PLG42_08015"
     CDS             complement(61..669)
                     /gene="rpsD"
                     /locus_tag="PLG42_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU31192.1"
                     /translation="MARYTGPKSRVARKFGDPIYGPDKHLDRKNFPPGQHGINKKRKK
                     TSEYGVQLREKQKAKYTYGVLERQFRNAFEKASRTKGVTGEVLLQILESRLDNVVYRL
                     GVAPSRASARQLVSHRHITVNGEVVSIPSYQLKAGDIIGVREKSKSLEVIVESLTTRK
                     SSKLSWLEWDDAQMAGKFMNIPVRADIPEDIKEQLIVELYSK"
     gene            complement(713..1102)
                     /gene="rpsK"
                     /locus_tag="PLG42_08020"
     CDS             complement(713..1102)
                     /gene="rpsK"
                     /locus_tag="PLG42_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU31193.1"
                     /translation="MAKKTGALKKRIVKVEPAGQAHVHSSFNNIIITLTNNSGQVISW
                     ASAGKMGFKGSKKNTPYAAQMASEECGKVAYDLGLRKVKVFVKGPGSGRESAIRAIAN
                     SGIEVTEIIDVTPLPHNGCRAPGRRRV"
     gene            complement(1110..1487)
                     /gene="rpsM"
                     /locus_tag="PLG42_08025"
     CDS             complement(1110..1487)
                     /gene="rpsM"
                     /locus_tag="PLG42_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU31194.1"
                     /translation="MARIVGVDLPRHKRGEVGLTYIYGVGRSTAQKVLDEAGVSRDAK
                     VEEWTDEQINSIRRVLNENYKLEGELRSETQLNIKRLMDIGCYRGVRHRLGLPVRGQS
                     TKNNARTRKGRKKTVANKKKATK"
     gene            complement(1526..1642)
                     /gene="ykgO"
                     /locus_tag="PLG42_08030"
     CDS             complement(1526..1642)
                     /gene="ykgO"
                     /locus_tag="PLG42_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOU31195.1"
                     /translation="MKVRASIRKRSADCKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(1657..1875)
                     /gene="infA"
                     /locus_tag="PLG42_08035"
     CDS             complement(1657..1875)
                     /gene="infA"
                     /locus_tag="PLG42_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU31196.1"
                     /translation="MAKQPSIEQDGTIIEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVKVEMSPYDLTKGRITFRYK"
     gene            complement(1880..2656)
                     /gene="map"
                     /locus_tag="PLG42_08040"
     CDS             complement(1880..2656)
                     /gene="map"
                     /locus_tag="PLG42_08040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU31197.1"
                     /translation="MLYLKTDEEIELLRESNLLVSKTLAEIARHIQPGITTLYLDEIA
                     ETFIRDNSAVPAFKGYNGFPKTLCTSVNDEVVHGIPSGYYLKEGDIISVDCGVILNGW
                     YGDGAYTFAVGEIKEEVKKLIEFTKASLHEGVKEAVAGNRVGDISNAVQSKAESGGFS
                     VVRELVGHGLGKRLHEPPEVPNFGRKGSGPKMSRGLVICIEPMINLGRKETVQSRDGW
                     TIRTADGKPSAHFEYAVAVRKEKADVLTTFDFVEEVINKM"
     gene            complement(2679..4010)
                     /gene="secY"
                     /locus_tag="PLG42_08045"
     CDS             complement(2679..4010)
                     /gene="secY"
                     /locus_tag="PLG42_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU31198.1"
                     /translation="MRLIQTIRNILKIEDLRARLLYTLGIILLYRLGKYITLPGIDPA
                     QLEGLRNQTSSGLMGLLDMFSGGAFSQASVFALGIMPYISASIVIQLLGIIFPYYQKL
                     QKEGESGRRKMNQHTRYLTVLILILQAPTYLVNLHAQLPESAFILKGTFFQISSVIIL
                     TAGTIFVMWLGEKITDKGIGNGISLIIMIGIIARMPANFIFESGTRLNGAGGAIGLIV
                     EIVILFVVVLGTIMLVQGTRRVPVQYARRIIGNKQYGGVRQYIPLKVNAAGVMPIIFA
                     QAIMMLPIIIAGFSTDSSSGFVVAFSNMYGFWYNLVTAILIIAFTYFYTAITINPVQM
                     AEDMKKNGGFIPGIKPGRKTVEFFDMIMSRITLPGSIFLAIIAILPSVAVKAGVTPQF
                     AQFYGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRVKGRSGAVTSI"
     gene            complement(4015..4461)
                     /gene="rplO"
                     /locus_tag="PLG42_08050"
     CDS             complement(4015..4461)
                     /gene="rplO"
                     /locus_tag="PLG42_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU31199.1"
                     /translation="MDLSNLKPAKGSVKKNRRLGRGQGSGRGGTSTRGHKGAKQRSGY
                     KKKIGFEGGQMPLQRRVPKYGFKNINRKEYKGINLGVLQNLADKKNLDKIDPEVMISA
                     GLISKNSLVKILGKGTLERKLEVHAHAFSKSAVTAIEAKNGAVVKI"
     gene            complement(4488..4667)
                     /gene="rpmD"
                     /locus_tag="PLG42_08055"
     CDS             complement(4488..4667)
                     /gene="rpmD"
                     /locus_tag="PLG42_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU31200.1"
                     /translation="MAKIRITQVKSENRKPERQKRTLLALGIRKLNHSVEHEATPQIL
                     GMVVKVKHLVRVENI"
     gene            complement(4681..5042)
                     /locus_tag="PLG42_08060"
                     /pseudo
     CDS             complement(4681..5042)
                     /locus_tag="PLG42_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
BASE COUNT         1282 a         1213 c          923 g         1624 t
ORIGIN      
        1 cattattact ttatcgggct tctggaatga taatatggcc atagaattta tcttagatta
       61 ttattttgaa tacaattcga ctatcagttg ttcctttata tcttccggaa tatcagcacg
      121 tacaggtata ttcatgaatt tgccggccat ttgtgcatca tcccattcaa gccatgaaag
      181 ctttgatgat tttcttgttg taagcgattc aacaattact tcaagtgatt ttgatttctc
      241 gcggacgcca attatatccc ctgccttaag ctggtatgac ggtatactaa caacctctcc
      301 gttcacagta atgtgccggt gtgacacaag ctgacgtgcg gatgccctcg aaggggcaac
      361 tccaagcctg tatactacat tatcaagcct tgattcaaga atctggagaa gaacctctcc
      421 tgtaacgccc ttggttctgg atgctttctc aaatgcatta cggaactggc gctcaagaac
      481 accgtaggta tacttggctt tttgtttctc cctcagctga acgccgtatt cggaagtttt
      541 ctttctcttt ttgtttattc catgctgccc cggaggaaaa ttctttctgt ccaggtgttt
      601 gtctggaccg tagattggat ccccgaattt tcttgcgact ctcgattttg ggccggtata
      661 tcttgccatt acactaatta tttatatcag acaaaaaatc tattctaatt cattatactc
      721 ttcttcttcc tggagctctg caaccgttat gggggagtgg tgtaacatct attatttcag
      781 tcacctctat tccggaattg gctattgcac ggatagctga ctcccttccg gaaccagggc
      841 ctttaacaaa tacttttact tttcttaatc caaggtcata agctacttta ccacattctt
      901 cagatgccat ctgtgctgca tagggggtat tctttttgga acccttaaaa cccatctttc
      961 cggctgatgc ccacgaaata acctgtccgg aattatttgt cagcgttatg attatgttat
     1021 tgaaagatga atgaacatga gcctgtccgg ctggctcaac tttaactatc ctcttcttta
     1081 atgctccggt cttttttgcc atagtctgtt tatttagtag cttttttctt gttagcaaca
     1141 gttttctttc ttcctttcct tgttctggcg ttattctttg tactctgtcc tctcaccggg
     1201 agtccaagtc tgtgccgtac accacggtaa cagccaatat ccataagtct tttaatattg
     1261 agctgtgtct cggaacggag ttcgccctcc agtttgtagt tttcgttaag gacgcgacgg
     1321 attgagttaa tctgctcgtc agtccactcc tcaactttag catcacggct cacgccagcc
     1381 tcatcaagta ccttctgggc tgtgcttctg cctactccat atatataggt cagacccact
     1441 tcgcctcttt tatgtcttgg taaatctacc ccaacaatac gtgccatata ctagttttct
     1501 taaataggtt gcaaaattaa tcaaattatc cctgtctctg tttgaacttg ggatttttct
     1561 tgttaatcac gtaaagacgt ccttttcttc tgactatctt acaatcagca ctacgctttc
     1621 ttatagatgc tcttactttc attaccgtaa tatttcttat ttatatctga atgttattct
     1681 tcctttagta agatcgtatg gtgacatttc cactttcact ttatcaccgg gcagtatttt
     1741 tatgtaatgc atccgcattt tgcccgagat atgagcagta ataacatgcc cgttctcgag
     1801 ctctaccctg aacattgcat tcgacagagc ttcgatgatt gtaccgtcct gctcaattga
     1861 tggttgttta gccatactat tacattttat ttataacttc ttcaacgaaa tcaaatgtcg
     1921 ttaatacgtc tgccttctct tttcttactg caactgcata ttcaaaatgt gcagaaggtt
     1981 ttccatcagc ggttcttatt gtccagccgt ctctcgactg taccgtctcc ttccttccga
     2041 ggttgatcat gggttcgata cagatgacca atcccctgga catcttcgga cccgatcctt
     2101 ttcttccgaa attaggaact tccggaggtt cgtgaagtct ttttccaaga ccatgaccaa
     2161 caagttccct tacaactgaa aatcctccac tttcggcttt tgactgtaca gcatttgaga
     2221 tatcaccaac cctgttgcct gcaacagcct ctttcacacc ttcatgcagt gaagcttttg
     2281 taaactcaat aagcttttta acctcctctt ttatctctcc gacagcgaat gtataagccc
     2341 catcgccata ccatccgtta agaatcactc cgcaatcgac agaaataata tcaccctctt
     2401 tcagataata ccctgaaggg ataccatgaa ccacttcatc attgaccgat gtacagagag
     2461 ttttcgggaa accattataa cccttgaacg ccggaaccgc tgaattatcc ctgataaatg
     2521 tttctgcgat ctcatcaaga taaagagttg taatccccgg ttgtatgtgt ctggcaatct
     2581 cagctaatgt tttagacacc agtagattgc tttctctcaa caactctatc tcttcatcag
     2641 tctttaaata caacatcaaa gaacgtaata ctttatatct aaatcgacgt aacagcacct
     2701 gatcttcctt taacccggcc tgatttcatc aatccatcat aatgacgcat aagcagatga
     2761 ctttcgatct gttgcaaagt atcaaggacc actccaacaa gtatcagaag agaagtccca
     2821 ccataaaact gagcgaactg gggtgtcacg ccggctttta cagcaactga tggaagtatt
     2881 gctataattg caagaaatat tgatccgggt agcgtgatcc tcgacattat catatcaaaa
     2941 aattcaacag tcttcctgcc cggcttaata ccgggtatga aacccccatt ctttttcata
     3001 tcctcagcca tctggaccgg attgatcgtg atagccgtat agaaatatgt aaaagcaatt
     3061 atcagtatcg ctgttacaag attataccag aatccgtaca tatttgagaa agcgactaca
     3121 aaacctgaac ttgagtcagt ggagaatccg gcaattatta ttggcagcat cattattgcc
     3181 tgggcaaata ttattggcat tactcctgct gcatttactt tcaaaggtat atactggcgc
     3241 acaccgccat attgtttatt gccgattatc cttctcgcat actgtactgg cacacggcgg
     3301 gtgccctgaa caagcatgat ggtgccgagt acgacaacaa acaggatcac tatttcaaca
     3361 ataaggccta ttgcaccacc ggcgccattg agccttgtgc ccgattcaaa gatgaaattc
     3421 gctggcattc tggctatgat ccctatcata atgattagcg atatcccgtt tcctatcccc
     3481 ttatctgtta tcttttcccc cagccacatg acaaatattg ttcctgcagt aaggattatc
     3541 actgaggaaa tctggaagaa ggtccctttc aggataaaag cagattcagg aagctgggca
     3601 tgcaggttga caagatatgt cggagcctga agaattagaa taagaacagt aagatacctt
     3661 gtatgctgat tcattttacg tctcccgctc tctccttctt tctgcagctt ctgatagtac
     3721 ggaaaaataa ttccgagaag ctgtatcacg atagaagcag atatataggg cataataccc
     3781 agagcaaaca ctgaagcctg tgaaaaagct ccacccgaga acatgtcaag cagacccatc
     3841 aagcctgaag aagtctggtt acgcaggccc tccagctgtg ccggatctat gccgggcaga
     3901 gtgatatatt tccctaacct gtataacaaa ataattccca gtgtatagag caatctcgca
     3961 cgaagatctt ctatcttaag gatatttctt attgtctgaa tcagtctcat gaaattagat
     4021 ttttacaacg gctccgtttt tggcttcaat ggctgtaact gctgatttac tgaatgcatg
     4081 agcatgaact tccagttttc tttcaagtgt tcctttcccg agtattttaa caagtgaatt
     4141 ttttgatatc agccctgctg agatcatcac ttcgggatca attttatcaa gattcttttt
     4201 gtcagcgagg ttttgtagca cacccaggtt gatgcctttg tattccttgc ggttaatatt
     4261 cttaaaaccg tatttgggaa cgcgtctctg aaggggcatc tgacctcctt caaaaccgat
     4321 cttcttttta tatccggaac gctgtttggc tcctttatgc cctctcgtgg aggtaccacc
     4381 cctgcctgaa ccctgtccgc gaccaagacg cctgtttttt ttcaccgatc ctttagcagg
     4441 ctttaagttg ctcaaatcca tgatcttatt tatatatcgt taatacctta aatattctct
     4501 acccttacca ggtgttttac cttgacgacc attcccaata tctgcggagt ggcttcatgc
     4561 tcaacactat ggttcagttt tctaatacca agagccagca aagtcctttt ttgtctttcg
     4621 ggcttccggt tttcgctttt cacctgggtg attctgatct ttgccatctg tgaaatagat
     4681 ttaaccatta aaaactcttt ccatattaat ccctctgtgt tcggcgaccg aatatggatc
     4741 gcgtatctgg agcaatgctg caaaagtagc ttttaccaga ttgtgggggt tcgaggagcc
     4801 ctttgattta gcgagaacgt tcttgacgcc tacgctttcg agcacggcac gcatggcacc
     4861 tcctgccttt actcctgtgc cttctgaagc tggcttgata aacacctttg caccaccaaa
     4921 tttagcttcc tgctgatgag ggatagtacc gtttataaca ggaaccttaa taaggttttt
     4981 cttggcatct tcaatacctt ggctatcgca gttgtaactt cactggcttt accaagacca
     5041 tg
//
LOCUS       DAOSNB010000192         5036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16783_length_5036_cov_3.920498, whole genome shotgun sequence.
ACCESSION   DAOSNB010000192 DAOSNB010000000
VERSION     DAOSNB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16783_length_5036_cov_3.920498"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLG42_08065"
     CDS             <1..1221
                     /locus_tag="PLG42_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOU31201.1"
                     /translation="LLEVEEINADLLKRIEKDNPASLPDEKTIILNYERDTLYTSDEE
                     GAIKIDEDVLDRLWRLGRITYRQEPYDVVGLLYAESANRFAVIAAATDAAGLLRLKNL
                     RLILTAVCVTSLFLFAVAGWFYSGRALKPISDVVKRVENISITSLHLRVDEGNGKDEL
                     GRLAKTFNKMLERLETAFMVQKEFIANASHELRTPLSSLNAQIEVLLMKDRSTEEYKK
                     ELVSILEDIRSLTELANKLLLIARTASVNPADLTARVRIDDVLWQVQEEMGKYKSDYT
                     ININLDESLTDSDQIVVTGDEFLLKTAFNNIIDNACKYSPDKKADLFLSLTGKMVCVE
                     IKDRGMGISSEELNKIFEPFYRGSNVKTVPGHGIGLSLVNQIVKSHNGKLEIFSVPGE
                     GTTVKVFLPVAENQ"
     gene            1566..2669
                     /locus_tag="PLG42_08070"
     CDS             1566..2669
                     /locus_tag="PLG42_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOU31202.1"
                     /translation="MGKSVKKNIHKIAVIGAGHGGLAMAGHLAIMGHQVNLYNRSEER
                     LWGVKSSGTIEVTGEIHGFGKIYLATTNMKEAIEGVELIMVVVPAYAHKWLAEQMAPH
                     LVDGQIVVLHPGRTFGAIEFKQVLQRMKVKADVIIAEAQTFIYASRATGPSQVHIFRI
                     KHSIPVASIRAHLIPYVIDKLRQFYPQFVPGDNVFKTSFENIGSVFHPALCVLNAGWI
                     EHDVDFQFYHEGATISVTKVLERIDMERVKVAEALGIRAISARQWLYMAYSVEGNTLY
                     EAMKKNVGYRGILAPKSLKVRYVEEDIPCSLVPIASVGKMLKVDTPAINSVIYLGSAL
                     NECDYWSNGRTVENLGIAGMSLRDLRLLAIGEK"
     gene            2682..4310
                     /locus_tag="PLG42_08075"
     CDS             2682..4310
                     /locus_tag="PLG42_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31203.1"
                     /translation="MKTIIGAAIGSCVHVSGLYHFLKLAEAEGYKAESLGPAVPVSRL
                     IKIIGEKKPDIVALSYRLTPETAAEIFSDIKVALKNTGLEGIRIIFGGTPPVAAEAVK
                     SGLFERVFDGTEPVEELKRYLRGGGVSDEKILFPDNLVERIEQKYPYPLIRHHFGRPT
                     IEETVEGAALIAEWGVLDVLSIGPDQNAQEHFFHPEEMDHSQDGAGGVPLRKPEDLEA
                     IYKATRRGNFPLVRCYAGTRDLLKWAEMSVKTINNAWGAIPLTWYSMLDGRSKRPIPE
                     AIRENQEVMRWYAQRGIPVEVNESHQWSLRDAHDTLAVAMAFLAAYNAKKAGVKYYVS
                     QYMFNTPPGTSAAMDIAKMLAKNEMIEELSDNEFKVFRQVRAGLAHLSPEPSIAMGQL
                     AASAVISLALKPHILHVVGYSEGDHAIKADELIQSCNIIHGVLRNVLYGMPEMTNDRK
                     VQERKNELKAEAKVLLDELWRRGEGKSEDPWTDASNIYDAIKTGLLDTPHFQGIPYLC
                     GKIITRIIDGACYAVDRDTGEKLTEKERIMELNH"
     gene            4318..>5036
                     /locus_tag="PLG42_08080"
     CDS             4318..>5036
                     /locus_tag="PLG42_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31204.1"
                     /translation="MANYIILVLCLIVFLAYIFDITSKYTRIPGVILLILLGIVIQIL
                     TDITGLKIPNLKPFLPVIGTIGLILIVLEASLDLKLERKKSRLILNSVISAATLFLVF
                     TAIMTFIIRKFMHYSLHDSLLNTIPFGVISSAIAISAAINLPADKREFIVYESSISDI
                     IGILVFDFVLINNGNVIKGIFTNFILGGILTLIIAIIITAALAYLLYKSKYHVNYVII
                     LTAVICVYVMAKLVHLPALFL"
BASE COUNT         1545 a          868 c         1170 g         1453 t
ORIGIN      
        1 ctgcttgaag tggaggaaat taatgccgat cttttgaaga gaattgagaa agataacccg
       61 gcaagtcttc ccgatgagaa aaccataatt cttaattatg agagagatac tttgtatacc
      121 tctgatgaag aaggtgcaat aaaaattgat gaagatgtcc tcgacaggct ctggaggctt
      181 ggaagaatta cctaccgcca ggagccttac gatgtagttg gtttgctgta tgctgaaagc
      241 gcaaacaggt ttgcggttat tgctgcagca actgatgctg caggattgct gaggttgaaa
      301 aacctgagac ttattctgac agcagtttgt gtaacgagtc tatttttatt tgctgtggca
      361 ggatggtttt attcaggccg ggctctcaaa ccaatttcag atgtggtaaa aagggttgaa
      421 aatatttcaa ttaccagcct tcatctcagg gtcgatgaag gcaatgggaa ggacgaactg
      481 ggaaggcttg caaagacatt caacaaaatg ctcgaaagac ttgaaacggc atttatggtt
      541 cagaaggagt ttatagcaaa tgcatcacat gaattgagaa cacctctctc atcactaaat
      601 gctcagattg aggtacttct gatgaaggac cgttcaaccg aagaatacaa aaaagagctc
      661 gtttcaattc ttgaggatat caggtcgctt acggaacttg ccaacaaact attgcttata
      721 gcaaggactg cttcagtaaa tcctgctgac ctcacagcaa gggtgaggat tgatgatgtg
      781 ctttggcagg tacaggagga gatggggaaa tacaaaagtg attatacaat aaatatcaat
      841 cttgatgagt ctctaactga ttcggatcag attgttgtaa ccggtgatga gtttctctta
      901 aaaacggcat tcaataatat aattgataat gcatgcaagt attcacctga taaaaaggca
      961 gatttatttc tcagcctgac aggaaagatg gtatgcgttg aaattaaaga cagaggaatg
     1021 ggaatatctt ctgaagaact gaataaaatc tttgaacctt tctacagggg tagcaatgtt
     1081 aaaactgttc cggggcatgg cataggattg tctcttgtaa atcagatagt taaaagtcat
     1141 aatggaaaac tcgaaatttt ttcagtaccc ggagaaggta ccactgttaa ggtttttctg
     1201 ccagtggcgg aaaaccagta aatagcggag tagataagta gtaagtttgc ttagaatttc
     1261 taaagtaatt aaattctgtc gcatactgtt aaatattttt ttacagcgtt ttgggtattt
     1321 aatgaatttt taatttcatt ttaaagttta tttaatatag atttccgata ttggctgtgg
     1381 caacttttcg gatcctgttc tgtaataatg ttctttctgg cctttttact ttgtttttca
     1441 gtaagagccc gtcacacgac gggctcttgc ttcctaatta aaaataatat aaactcgtta
     1501 ttatacatta tatttcacta aatttgaatt tattagagct acaggagttg ataataaata
     1561 atgtcatggg aaaatcggtt aaaaagaaca ttcataagat tgcagttatt ggtgccgggc
     1621 atggtggact tgcaatggca ggtcatctgg caataatggg gcatcaggta aatctataca
     1681 atcgcagtga ggaaagactc tggggtgtaa agtcatcagg tacaatagag gtgacaggtg
     1741 agattcatgg tttcggtaaa atctatctgg caaccacaaa tatgaaagaa gctattgaag
     1801 gggttgaact tattatggtt gtcgtaccgg catatgctca taaatggctg gcagaacaga
     1861 tggctccaca tcttgttgat ggacagattg ttgttcttca tcccggaaga acctttggag
     1921 ctattgaatt caagcaggtt ctgcaaagaa tgaaagtgaa agcagatgtg attattgccg
     1981 aagcacagac ttttatttat gcttcccggg cgacaggtcc atcccaggtt catattttca
     2041 gaatcaagca ctcaatacct gttgcttcca tcagggcaca tcttatcccc tatgtaatag
     2101 ataagttaag gcagttttat cctcaatttg ttccgggtga caatgttttc aaaaccagct
     2161 ttgagaatat tggtagtgtt tttcacccgg cactgtgtgt acttaatgca ggttggatag
     2221 aacacgatgt tgattttcag ttttaccatg agggagcaac tatatcagtt acaaaagtgc
     2281 tggaaaggat tgacatggag cgtgtcaagg ttgctgaggc tctggggata agggcaattt
     2341 ctgcccggca atggctttat atggcataca gtgtggaagg gaatactctt tatgaagcta
     2401 tgaaaaaaaa tgtgggatac agaggtattc tcgctcccaa atcattgaaa gtgagatatg
     2461 tcgaagaaga cataccatgc agccttgtac ctattgcatc agttggtaaa atgctgaaag
     2521 ttgatactcc tgcaataaat tcagtgatat acctcggttc tgcactgaat gagtgtgact
     2581 attggtcgaa tggcaggaca gtcgaaaacc taggcatagc agggatgagt ttaagggacc
     2641 tgagacttct tgcaattggt gaaaaataaa ttttcgggaa gatgaaaaca attattggtg
     2701 ctgccatcgg cagttgtgtg catgtaagcg gtctttatca ttttttgaaa ctggcagagg
     2761 cagaaggtta taaagctgaa tccctcggac cggcagttcc ggtgtcgcgg cttattaaga
     2821 taataggtga gaagaaacct gatattgttg cattaagtta ccggctgaca cctgagacgg
     2881 cggcagaaat tttttctgat attaaggttg cgcttaaaaa tacagggctg gaagggataa
     2941 gaataatatt cggaggcact cctcctgttg ctgcagaagc ggttaaatca gggcttttcg
     3001 aaagggtttt tgacggaacg gaaccggttg aggagttaaa aagatacctg cggggcggag
     3061 gtgtttctga tgagaaaatc ctgtttcccg ataatcttgt ggaaagaata gaacaaaaat
     3121 atccttatcc tctgatacga caccattttg gaaggccgac tattgaagaa acggttgaag
     3181 gtgcagcact gattgctgag tggggggtgc tggatgtttt atcaatagga cctgaccaga
     3241 atgcccagga gcatttcttt catcctgaag agatggatca tagccaggac ggtgcaggtg
     3301 gtgtgccgtt aagaaagccg gaagatctgg aagctatata caaagcgacc cggaggggca
     3361 actttcccct tgtcagatgt tatgctggca cgcgcgatct gcttaagtgg gctgagatga
     3421 gcgtaaaaac aataaacaat gcatggggag ctattccact tacatggtat agtatgctcg
     3481 acggacgttc aaaacgcccg atacctgagg caataagaga gaatcaggaa gtgatgcgat
     3541 ggtatgccca aagagggata cctgttgaag taaatgagtc gcaccagtgg agtctacgcg
     3601 atgcacatga tacgcttgca gtggcaatgg ctttcctggc tgcttataat gccaaaaaag
     3661 ctggcgtaaa atattatgtg tctcaatata tgtttaatac tcctccaggc accagcgctg
     3721 ctatggatat tgcaaaaatg ctggcaaaaa atgaaatgat tgaagaactg agtgataatg
     3781 agttcaaagt attccgtcag gtaagagccg gccttgctca tctgtcaccc gaaccatcaa
     3841 tcgcaatggg tcaactggca gcatcagcag tgataagtct tgcattaaag cctcatatac
     3901 ttcatgttgt tggatacagc gaaggtgatc atgcaattaa ggccgatgaa cttattcaga
     3961 gttgtaatat tattcatggt gttctaagaa acgtactcta cggaatgcca gagatgacaa
     4021 acgacagaaa agttcaggaa agaaaaaatg aacttaaagc tgaggcaaaa gtgctcctcg
     4081 atgaactatg gcgcaggggt gagggtaaat cagaagatcc ttggaccgac gccagcaata
     4141 tttatgacgc aataaaaacg ggattgcttg atacaccaca tttccagggc atcccttatc
     4201 tgtgcgggaa aataataacc aggataatag acggcgcctg ttatgcagtt gacagggata
     4261 caggagaaaa gcttactgaa aaggaaagaa taatggaact taaccattaa ataaaaaatg
     4321 gcaaactata taattcttgt tctatgttta attgtttttc tggcttatat atttgacata
     4381 accagtaaat ataccaggat acccggagtc atactactga tcttactggg catagttatt
     4441 caaatactaa ctgatataac cggcctgaag attcccaatc taaaaccatt tttacctgtt
     4501 attggcacaa tcgggctgat tcttattgtc cttgaagcaa gccttgatct gaagctggag
     4561 aggaagaaat caagattgat attaaattct gtgatttcag cggctaccct ttttctggtt
     4621 tttacagcaa taatgacatt tattattcgt aaattcatgc attacagttt gcacgattcc
     4681 cttcttaaca ccataccatt tggagtgatt agcagtgcaa ttgccatttc ggccgctata
     4741 aaccttccgg cagataaaag ggagtttatc gtatatgaaa gttcaatctc agatataatt
     4801 ggtattctgg tgtttgattt tgtattgatt aataatggca atgtaattaa agggattttt
     4861 acaaatttta tacttggagg catactaact ctgataattg caattattat tacagccgct
     4921 cttgcatatc ttttgtataa atcaaagtat catgtgaatt atgtgattat tctgacagca
     4981 gtcatctgcg tatatgttat ggcaaagctg gtacatctgc ctgctctttt tctgat
//
LOCUS       DAOSNB010000193         4986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_16992_length_4986_cov_4.419793, whole genome shotgun sequence.
ACCESSION   DAOSNB010000193 DAOSNB010000000
VERSION     DAOSNB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16992_length_4986_cov_4.419793"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLG42_08085"
     CDS             <1..761
                     /locus_tag="PLG42_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31205.1"
                     /translation="IVRHWAGLKGEVVFDNTRKRLINIYYGTRFKIFGEYYKEVSEKL
                     SDMIVLGADFRHYLKIHRELVWANRFAASTSYGPTKLIYYMGGVDNWMGYLFSKIPMF
                     DKTIPVNPNYKYGFQALATNMRGFSQNIRNGNSFALINSEIRWPFIRYFAGHPLKSNF
                     LNSLQIVGFGDIGTAWSGKSPWSGENSYDNEIITNGPVTVILDTNRDPIVAGFGAGLR
                     AQLLGYFVRADWAWGIENGYVLPMVFYLSFSLDF"
     gene            892..1059
                     /locus_tag="PLG42_08090"
     CDS             892..1059
                     /locus_tag="PLG42_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOU31206.1"
                     /translation="MAYKISEDCTACGTCIDECPVGAISEGEIYKIDPDLCTDCGACA
                     DVCPVEAIHPA"
     gene            complement(1112..2140)
                     /locus_tag="PLG42_08095"
     CDS             complement(1112..2140)
                     /locus_tag="PLG42_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31207.1"
                     /translation="MKRYNGLIFLLIAAVMLLSACRRNQYRANISGIKVEVNIKRLEQ
                     DLFSVDPVKIPEMTGMLTEKYGNFLKYFSYVINIGEPGDSAWLDGLIRFCTDKLNNEV
                     YAKTMEVFPELNWLEKDLSTAFRYYRYYFPSKPVPAVYSCITGFNNSIITGDSTLGIG
                     LDRYLGKECKFYPQLGIYNYQAAKMTPSNIVTDCMYGWAASEWDYEVMGYSPDDVLSS
                     MIHEGKLLYFVKCMLPGLPDERIFGFTKEQMKFCIDNEGSMWQYLIENNLLFSADSFT
                     KKKLMGEAPFTSYFSKESPGRAAVWIGFRIVESYMRHNRNITLEELMSDKDTRAILEK
                     ARYSPKAV"
     gene            2267..2962
                     /locus_tag="PLG42_08100"
     CDS             2267..2962
                     /locus_tag="PLG42_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOU31208.1"
                     /translation="MNLLRKSSLRTYSAILVSLFTINAGVVTAQNIRFGIFADPVIGW
                     FSSDTRETKNEGARAGFNFGFTFNKYFANNYSFSTGLNILTAGGRLVSSDTIIMEFTN
                     FDTEVAAGKPVVYKIQYLNIPVGLKFETNQIGYVTFFADIGLDPKIAIGGKVDIPSAN
                     IKGERAMDEIRHFNMGYHILGGIDYSLGGTTAIVLGLGFEHNFLDVTKENNIQPRDRI
                     SHNLLKFRVGINF"
     gene            2974..4626
                     /locus_tag="PLG42_08105"
     CDS             2974..4626
                     /locus_tag="PLG42_08105"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOU31209.1"
                     /translation="MKITIAQLNYHVGNFERNSFLIKQAIKKASDEGADLVIFSELCI
                     TGYPPLDLLDHADFVKKCDETVKEIASCCRGITAIVGSPIINTRPEGKKLFNAALVLS
                     EGGIIFEARKALLPTYDIFDEYRYFEPERNFGIFEFKGKKIAITICEDLWDEQPFPNE
                     FEKTRLYTVSPAEELAKFNPELLINLSASPFSYTRTGAKRKIFTGKASKYNIPVIMVN
                     QTGANTELIFDGGSMVISKNGRIIRQLPFFEEAVYTFDYEDLLSVTDEADPPLPDQIE
                     LIYKALITGIKDYFGKSGFSKAVLGLSGGIDSATVLCLAVEALGAENVKALILPSRYT
                     PARSLDDAVKMAQNAGVSYEVISIEKPFMAFEEVLKPIFRGLPPDVTEENIQARIRAV
                     LLMAYSNKFGHILLNTSNKSEAAVGYGTLYGDMAGGLSVIGDVYKTDIYRLAKYINRN
                     SEVIPESILTKPPSAELRPDQLDTDSLPEYPVLDGILYRYIEKQMPVSSIIAEGFDAE
                     TVLKTIRMINSNEYKRYQAPPILRISQKAFGYGRRMPIVAKY"
     gene            complement(4652..>4986)
                     /locus_tag="PLG42_08110"
     CDS             complement(4652..>4986)
                     /locus_tag="PLG42_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HOU31210.1"
                     /translation="EPVGYGCTDISDRERRIAVLPVGYADGLDRRLGNGRGRFYINGH
                     FVPVVGNICMDSCMADVTGIDASEGDEVEIFGEHISVEDLARIIDTIPYEILTSVPPR
                     VKRVFYRE"
BASE COUNT         1522 a         1043 c         1023 g         1398 t
ORIGIN      
        1 atattgtaag gcactgggca ggccttaagg gtgaagtggt atttgataat acccgcaaga
       61 gactcataaa catttattac ggaacaagat ttaaaatttt cggagagtac tacaaagaag
      121 tgtctgaaaa actgtcggat atgattgtcc tgggagctga tttcaggcat tatcttaaga
      181 tacaccgtga gttggtatgg gcaaacaggt ttgccgccag cacatcgtac ggacctacaa
      241 aacttatcta ctacatggga ggcgtggaca actggatggg atacctcttc agcaaaatac
      301 caatgtttga caaaacaata ccagtaaatc ctaattacaa atatggtttt caggcacttg
      361 ccacaaacat gagggggttc agccagaata ttcgaaacgg aaacagtttt gcactgatta
      421 acagcgaaat acgctggcca tttataaggt attttgctgg ccatccgcta aagtcaaact
      481 tcctcaacag cctgcagata gtaggtttcg gcgacattgg gactgcatgg tcgggaaaat
      541 ctccgtggtc aggagaaaat tcgtacgata atgaaataat tactaacggg cctgttacag
      601 ttatccttga taccaaccgt gatcccattg tggcagggtt cggggcaggg ctaagggcac
      661 agctgctggg ttatttcgta cgtgccgact gggcgtgggg aattgaaaac ggatatgtgt
      721 tgccaatggt cttctatctt tccttcagct tggatttcta agtgtaataa aaaagctgag
      781 agattattaa aattttactg ccataaattc aattaataat atttttcatt tcagatttca
      841 aaaaaatagg ttatttttgg cgtctgctta tattaaccaa aaataaaagt catggcttac
      901 aaaatcagtg aagattgcac agcatgcgga acctgtattg atgaatgtcc tgtaggtgca
      961 atatcggaag gggaaattta taaaattgac cccgatttgt gtaccgattg cggtgcatgt
     1021 gccgatgtat gcccggtaga ggctattcat cctgcataag tctgcaatca agaaataaga
     1081 gggtctctaa tagggaccct tttttatttt atcacaccgc tttaggactg taacgtgctt
     1141 tttcgagtat tgcccttgta tctttatcgc tcataagctc ctcaagggtt atgttcctgt
     1201 tgtgcctcat atacgattcc acaatccgga agcctatcca tacagcagca cggcccggcg
     1261 actctttcga aaaataactc gtaaaaggtg cctcacccat cagttttttc ttagtaaacg
     1321 aatcggcact gaaaagcagg ttgttctcaa taagatactg ccacatgctt ccttcattat
     1381 caatacagaa tttcatctgc tcttttgtaa aaccgaaaat cctttcatct ggcagtccag
     1441 gcagcatgca tttcacaaaa tagagcaact ttccttcgtg aatcatagag gagagaacat
     1501 catcggggct gtatcccata acttcataat cccattccga ggctgcccat ccatacatac
     1561 agtcggttac aatatttgaa ggtgtcattt ttgcagcctg ataattgtag attccaagct
     1621 ggggataaaa cttacactct ttaccaaggt acctgtcgag cccgatacca agtgttgaat
     1681 cgccagtaat tatactgttg ttaaaaccgg taatgcatga ataaactgca ggaacaggct
     1741 tcgatggaaa atagtaccta taatacctga atgctgttga taaatctttt tcgagccaat
     1801 taagctcagg aaatacttcc atggtttttg cataaacctc attgtttagc ttgtcggtac
     1861 agaaccttat caacccatcg agccatgctg aatctcccgg ttcgccaatg tttattacat
     1921 aactgaaata ctttaaaaaa ttaccatact tttcagtcag cataccagtc atttctggta
     1981 ttttaacagg gtcgaccgaa aagagatcct gctcaagtct ttttatattt acttcgactt
     2041 taatcccgga aatatttgcc ctgtactgat ttctcctgca ggcagaaagt aacatcactg
     2101 ctgcaataag taaaaatatc agaccgttgt atcgtttcat aacctgcaaa tgtattgcgc
     2161 gaaattattc actttttgac agattgccaa gatgaaccag caaaacagta atttctgcca
     2221 ttatttgtta tttttacagg cgtttaatca attatttaat gagtttatga atctactaag
     2281 aaaatcttca ctcagaactt attcagcaat tctggtatca ctcttcacca ttaatgcagg
     2341 tgtggtcacg gcacagaaca tccggttcgg catttttgct gatcctgtta ttggctggtt
     2401 ctcatcagat acccgtgaaa caaaaaatga aggagcaaga gcaggattca attttggctt
     2461 caccttcaac aaatacttcg caaataacta ttctttttca acaggtttaa atattcttac
     2521 tgcgggtggc agattggtaa gcagcgacac aattataatg gaattcacaa attttgatac
     2581 tgaagtagct gcagggaaac ctgttgtgta taaaattcaa tatcttaata ttcctgtagg
     2641 gctaaaattc gaaacaaatc aaataggtta tgtcaccttc tttgctgaca taggacttga
     2701 tcctaaaatt gccatcggcg gaaaggtcga tattccatcg gcaaatatta agggtgagag
     2761 ggcaatggat gaaataaggc atttcaacat gggttaccat atactcgggg gaatagatta
     2821 ttctctcggc ggcacaacag ctattgtgct cgggcttgga ttcgaacaca actttcttga
     2881 tgtaacaaaa gaaaataata tacagccccg cgacagaata tcacataatc tgcttaaatt
     2941 cagggtggga attaactttt gaaaacagaa aatatgaaaa tcactatagc ccagcttaat
     3001 taccatgtgg gaaattttga gaggaacagc tttcttatta agcaagctat aaagaaagca
     3061 tcggacgaag gagcagatct tgtaatattt tcagaattat gcataacagg ttatcctcct
     3121 ctcgaccttc tcgaccatgc tgattttgta aagaagtgtg atgaaacagt taaagagata
     3181 gcatcatgct gcaggggtat tacagccata gttggttctc ccattattaa cacccggccc
     3241 gaaggcaaaa agctttttaa tgcagctctt gtactgagtg aggggggaat cattttcgaa
     3301 gcccgcaaag cattgcttcc gacatatgat atctttgatg aatacaggta ttttgaacct
     3361 gaaaggaatt ttgggatatt tgagttcaaa ggcaaaaaaa ttgccataac catttgcgag
     3421 gacctctggg acgaacagcc tttcccgaat gagttcgaaa aaacgaggct ttacacagtc
     3481 tcacctgccg aagagctggc aaaattcaat ccagagcttc tcataaacct ttctgcctca
     3541 cctttttcat acaccagaac aggagcaaag cggaaaattt ttaccggaaa agcatcaaag
     3601 tacaacattc cggtgataat ggtgaatcag accggggcaa acaccgaact gatctttgat
     3661 ggaggctcga tggtaatatc aaaaaacgga aggataatca ggcaacttcc tttttttgaa
     3721 gaggctgttt atacattcga ttacgaagac ttactttctg tcactgatga agctgaccct
     3781 cccttgccgg atcagattga actgatatac aaggctttga taacaggtat aaaagattat
     3841 ttcggtaagt caggtttctc aaaagcagta cttgggttgt cgggaggtat agattctgca
     3901 acggttttgt gccttgcagt tgaagccctc ggagcagaaa acgtaaaggc actcatcctg
     3961 ccttcgagat atactcccgc ccggtcgctt gatgatgcgg taaaaatggc acaaaatgca
     4021 ggtgtgagtt atgaagtcat cagcatagaa aaacctttca tggcttttga ggaagttctc
     4081 aaaccgatct tcagaggttt gcctcctgat gtgaccgaag aaaacattca ggcgcggata
     4141 agagctgttt tacttatggc ttattcgaac aaattcgggc atatacttct caatacatca
     4201 aataaaagtg aagcagcagt ggggtatggc actctttatg gtgacatggc gggaggactc
     4261 tcggtaatag gcgatgttta caaaactgat atttacaggc tggcaaaata tatcaaccgc
     4321 aactcggagg ttattcctga aagtattctc accaaacctc cttcagccga gctgaggcca
     4381 gaccagctcg atactgactc ccttcctgaa tacccggtac ttgatggaat actctaccgt
     4441 tacatagaga agcagatgcc agtctcgtca atcattgctg aaggattcga cgcagaaacg
     4501 gtactgaaga caataaggat gataaattca aacgaataca aacgttatca ggcacctccc
     4561 atactccgta tctcacaaaa ggctttcggt tacggcagac gaatgccgat agtagcaaaa
     4621 tattaaaagg acttccagca tataccgtgt ctcactcacg gtaaaagact ctcttgaccc
     4681 gaggcgggac ggaagttaat atttcatacg gaatagtatc tattatacgt gccaaatctt
     4741 caacgctaat atgttcgcca aaaatttcca cctcatcacc ttcagaagca tctatgcctg
     4801 ttacatctgc catacacgag tccatacaga tattacctac aacaggcacg aaatgaccat
     4861 tgatataaaa tcttcccctg ccgtttccga gtctgcggtc gaggccgtcg gcataaccta
     4921 ccggcagaac agctatgcgc ctctccctgt ctgaaatatc tgtacaaccg taacctacgg
     4981 gttcgc
//
LOCUS       DAOSNB010000194         4966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17065_length_4966_cov_5.896762, whole genome shotgun sequence.
ACCESSION   DAOSNB010000194 DAOSNB010000000
VERSION     DAOSNB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17065_length_4966_cov_5.896762"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PLG42_08115"
     CDS             <1..1023
                     /locus_tag="PLG42_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU31211.1"
                     /translation="HLRNWKETPERYRALEYILNELAAKEIGNLIISGDVFDRDFNNY
                     TDFNELCSRFSKIRITIFPGNHDAEISKKYFPAQNLEVVNEATLKQFGNISILVLPYH
                     PGKLMDEVLTEFFYNNPVPERWLLTGHGDYCTTAKDFNPYEPGVYMPLSGSAINKFNP
                     FYVILGHIHKPSETGRVIYPGSPCPLDINETGKRRYIIFNTSDNSIEKLSVKTDVIYF
                     NETLMTFPSENEFHDICDSIDRMIKNWKLDDNEKGLVKLRLKIKGYTSDLSRLKESII
                     SHLEKKDIRLYDNDGPDLKEVNVVKEADDDRIILLKKTIEKISQLKIEADRVKILEKI
                     QEKIFT"
     gene            1030..3660
                     /locus_tag="PLG42_08120"
     CDS             1030..3660
                     /locus_tag="PLG42_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31212.1"
                     /translation="MNIRIAKISAENCGPVKKINIAPGNLTIFYGRNEDGKSFLVEFI
                     IRCLFKNKKPWGDLRQAGGGKITLTGIKDKPVDFIPDKGVKLEDYFERDSRGLPLSLL
                     NLLVVKEGETGIGNNKEELTKDIVKTFLSSRRLLDEIDGKIPATFKEAKIDTDIDIDK
                     RGGRDYYNKLEEIKKIDNIIYSLNNQLAHWLIRDLSLQENQLKCKKELLNRARKFKAY
                     TLSTKIKAVEKQLEKIPRETISELKELYNEYRKKQEKYELLKQELADIQKETELIPDL
                     ENKKELLLKAKRYQAYQLGSEIKNIEEKLRSLPETELNKLENNISIYRNKKNELESCK
                     KSINQASEKSADYHWLKTAKENYHRFLSTTGPNKKVSRAFPVAAGLTFITGIVTLLLN
                     QKIPGLIILIISALLTLLYFIFLKKSYSSLRHSEELKQIESEFKKRFGEELENITKLE
                     SIFNEEEKYFNQLEVYNTQLIRLSTETDSYFKLIQEMFAGFGIDNPPEEKWEEFISVI
                     RQNREDLTSKYDLLNRKLASLDTDETEYETKDPGVKFDRNELEEIKEHLTRLYELKKQ
                     ENKKIHERNELNNSLKVTGKVVTEKFRSFTEEEIAEEEWTSKIKLLENQYNNLLSYYE
                     SLKGQLTGLGVSESDFEKEDPGIEYSPEEMEKIEKKMEEISEKLKNEEERFATLKGEI
                     ISITGAETSAKWTDLVDMLYRKKQEIKDEVEGIKASIIAGKLLHLTIQELQLEEDQKL
                     EEALNSKEVTSVLQNLTGHYNALSFDDKKIIISDGYNNYYLKDLSTGALEQVMIALRI
                     GILKRLLKNDNAFLILDDAFQHSDYEKRHILVKTVCELARNGWQIIYFTMDDHILNLF
                     RKEKNGDYIEIDLHTHKLHN"
     gene            complement(3673..>4966)
                     /locus_tag="PLG42_08125"
     CDS             complement(3673..>4966)
                     /locus_tag="PLG42_08125"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOU31213.1"
                     /translation="GIWFTLGQFYEYGDKYSGGLGTYTANHIPIAIYSPQAGKTYFTY
                     GGTTKADERHLLIMVSYYDHMSKTVPRPVIVYDKNGVNDPHDNAAISIDEKGYIWVFV
                     SGRSRTRPGLIFRSQNPYDISAFEKIREGEMTYPQPWWVKGEGFLYLFTKYTRGRELY
                     WAKSKDGINWEPEQKLAGMGGHYQVTNMLGNKLVSVFNYHPGGDVDKRTNLYAVQTTD
                     MGKTWTTIDGRIIEPPLTEVHNEALVWDYESEKKLVYINDLNFDRDGNPVILAIISRD
                     FRPGPSGDPREWFIIHWKDNRWNFRKVCESTHNYDMGSLYTEGEEWKIIGPTEPGPYR
                     YGTGGEIAMWVSRDEGSTWIKVRNVTEGSLLNHSYVRRPVNAQKDFYALWADGNPDTL
                     SESHLYFTDIRGKKVWELPYTMRENREKPLPVKFGKTP"
BASE COUNT         1756 a          919 c          880 g         1411 t
ORIGIN      
        1 catctcagaa actggaaaga gactcctgaa agatataggg ctcttgaata tattttgaat
       61 gaacttgctg caaaagaaat tggtaatctt attatttccg gtgacgtatt tgacagagat
      121 tttaataact atacagattt taatgaacta tgctccagat tcagtaaaat aagaataact
      181 attttccccg gtaaccacga tgcagaaatc agtaaaaaat attttcctgc tcaaaacctg
      241 gaagtagtta atgaagcaac attaaaacaa ttcgggaata tttcaatatt ggttcttcca
      301 taccatccag ggaaactgat ggatgaagtt cttacagagt tcttttacaa caaccccgtt
      361 cctgaaagat ggttactgac cggtcatggt gactattgca caacagcgaa agattttaac
      421 ccatacgagc ctggtgtata tatgcctctt tctggttcag ctattaataa atttaatccc
      481 ttttatgtca ttcttggaca tattcataag ccctcagaaa ctggcagagt tatttacccc
      541 ggctctccct gcccactcga tataaatgag acgggcaaaa gacgttatat tatttttaat
      601 acatccgaca acagtattga aaaattatcc gtaaaaactg acgtcattta ttttaacgaa
      661 acccttatga cattcccatc agaaaatgag tttcatgata tctgcgactc aattgacaga
      721 atgattaaaa actggaaact tgatgacaat gaaaaaggtc ttgttaaatt aagactcaaa
      781 ataaaaggtt atacgtcaga tcttagcagg ctaaaagaat ccataataag tcatcttgag
      841 aaaaaagata taaggcttta tgataatgat ggaccggatc tgaaagaggt taatgtggtt
      901 aaagaggctg acgatgatag aataatcctc ctgaaaaaaa caattgagaa aatctctcaa
      961 ttgaagatcg aagccgacag agtaaaaatc ttagaaaaaa ttcaggaaaa aattttcact
     1021 tagaaaaaaa tgaacatacg catagcaaaa atttctgctg aaaactgcgg gccggtaaaa
     1081 aagatcaaca ttgcgcccgg taatcttaca atattctacg gcagaaatga ggacggcaaa
     1141 tcgttccttg ttgaattcat aatccgttgc ctttttaaaa acaaaaaacc atggggtgat
     1201 ttgcgccagg ctggtggtgg caaaattact ttaacaggaa ttaaagataa acccgtagat
     1261 tttatacccg ataaaggagt aaaacttgag gattattttg aacgggattc ccgtggttta
     1321 cctctttcac tgcttaacct tcttgttgta aaggaaggtg aaaccggaat tggcaataac
     1381 aaagaagaat taactaaaga tatagtaaaa acatttcttt cttcccgcag attacttgat
     1441 gaaattgatg gaaaaatacc ggcaactttc aaggaggcta aaattgatac agatatcgac
     1501 atcgacaaaa gaggaggaag agattattat aataaactcg aagaaattaa gaaaatagat
     1561 aacataatct attcgctcaa taatcagtta gcccactggc tgataagaga cctgtcgcta
     1621 caggaaaatc agctgaaatg taaaaaagaa cttcttaaca gggcacgtaa attcaaagca
     1681 tacactttat ccaccaaaat taaggcagtt gaaaaacaac ttgaaaagat tccccgtgag
     1741 acaatttccg aattaaagga actatataac gaatacagaa aaaaacagga aaaatatgaa
     1801 ttattaaaac aagagctggc tgatattcaa aaagaaaccg aacttattcc cgatctcgaa
     1861 aataaaaaag aactgctttt gaaagcaaaa agataccagg cttaccagct tgggtcagaa
     1921 ataaaaaata ttgaagagaa gctgagatcc cttcctgaaa ctgaattaaa caaactagaa
     1981 aataacattt ctatttatcg caataaaaag aatgaattag aaagctgtaa aaagtcaata
     2041 aaccaggcat ctgaaaaatc agcagattac cactggctga aaacagcaaa agaaaattac
     2101 cacaggtttc tctcaacaac aggtcccaac aaaaaagtat caagggcttt cccggttgca
     2161 gcaggattaa cttttataac aggtattgtt actttattat tgaatcaaaa aattcccggt
     2221 ctaattattc ttataatcag tgccttactt acccttttat attttatttt tttaaaaaaa
     2281 tcatattcat ctttaagaca ttccgaagaa cttaaacaaa tagaatctga attcaaaaaa
     2341 cgatttggag aggaacttga aaacattaca aagcttgaat ctatttttaa cgaagaagaa
     2401 aaatatttta accagcttga agtttataac actcagctga tccgtcttag tactgaaaca
     2461 gattcttatt ttaaattaat acaggagatg tttgcaggat ttggaattga taaccctcct
     2521 gaggaaaaat gggaagaatt tatttcggta atcagacaaa atagagaaga ccttacttcc
     2581 aaatatgacc ttcttaaccg taaactggca tcacttgata ctgacgaaac ggaatatgaa
     2641 actaaggatc cgggagtaaa atttgacaga aatgaacttg aagagataaa ggaacatcta
     2701 acaaggcttt atgagcttaa aaaacaggaa aataaaaaga ttcatgaacg gaatgaatta
     2761 aataatagcc taaaagttac tggtaaagtt gttactgaaa aattcaggag ttttacagag
     2821 gaagagatag ctgaagaaga atggacctca aaaattaagt tgcttgaaaa tcaatacaat
     2881 aatttattaa gttactacga gagccttaaa ggccagctca ccggattggg agtttctgaa
     2941 tcagattttg aaaaagagga tccgggcatt gagtattctc ctgaggaaat ggagaagatt
     3001 gaaaaaaaga tggaggagat tagcgaaaag ttgaaaaatg aagaggaaag atttgctact
     3061 ttaaaaggtg aaatcatatc tatcacaggt gctgaaacaa gtgctaaatg gactgacctc
     3121 gttgacatgc tgtacagaaa aaagcaggag ataaaagatg aagtagaggg aatcaaagcc
     3181 agtattattg ccggtaaact gcttcactta acaattcagg aactgcagct ggaagaagac
     3241 cagaaacttg aagaggcact taattccaag gaagttacat ctgtattaca aaatctgacc
     3301 ggacattata atgctctttc atttgatgac aaaaagataa taatctctga tggatacaat
     3361 aactattacc tgaaagatct gagcactgga gctctcgaac aggtaatgat agccctgcga
     3421 attggaatac tgaaacgact gcttaaaaat gataatgcct ttctgattct tgatgatgca
     3481 ttccagcatt cagattatga aaaaagacat atactggtta aaactgtatg tgaacttgcc
     3541 cgaaacggtt ggcagataat ctattttaca atggacgacc atattcttaa tcttttccgt
     3601 aaagaaaaaa atggcgatta catagaaatt gatttacaca ctcataaact tcataattaa
     3661 ttgaaaaatt cttcatggag ttttaccaaa ctttacaggc aacggctttt ctctattttc
     3721 tctcattgta tatggtaatt cccaaacctt ttttcctctt atatcagtaa aatagaggtg
     3781 agattcggaa agtgtatccg gattcccgtc ggcccataaa gcataaaagt ctttttgcgc
     3841 attcacaggc ctccttacat atgaatgatt tagtaaactg ccttctgtta cgtttcgaac
     3901 ctttatccag gttgagcctt catccctgct gacccacatt gcaatttccc ctcccgttcc
     3961 gtaacgatag ggaccaggct ccgttggccc aattattttc cattcttccc cttcggtgta
     4021 cagggacccc atatcataat tatgtgttga ttcacaaact tttctgaaat tccatctgtt
     4081 atctttccaa tgaattataa accattccct gggatcacct gaaggacccg ggcggaaatc
     4141 gcggctgata atagcaagta taaccggatt tccatctctg tcaaaattaa ggtcattaat
     4201 ataaaccagt tttttctcag attcataatc ccataccaat gcttcattat gaacttcagt
     4261 aagaggtggc tcaattatcc tgccatcaat tgttgtccag gtttttccca tatcagttgt
     4321 ctgtactgca taaagatttg ttcgtttatc tacatctccc cccggatggt aattgaatac
     4381 cgagaccagt ttgttcccta acatatttgt cacctggtaa tgtccaccca taccggcaag
     4441 tttctgttcg ggctcccagt ttatgccatc tttgcttttt gcccagtaga gttctctccc
     4501 gcgggtatat tttgtaaaca gataaagaaa tccttccccc tttacccacc atggctgggg
     4561 atatgtcatt tcaccctccc tgattttttc aaaagcgctt atatcatatg gattttgact
     4621 tctgaaaatt agcccgggcc ttgtccggct cctcccactg acaaaaaccc atatgtagcc
     4681 tttttcatca atagatatag ccgcattatc gtgtgggtca ttcacaccgt ttttatcgta
     4741 aacaattaca ggcctgggaa cagttttact catgtggtca taatatgaca ccataatcaa
     4801 aaggtgtctt tcatcagctt ttgtagttcc cccataggta aagtaagttt ttcctgcctg
     4861 aggagaataa attgcaatcg gaatatgatt tgcagtataa gtcccgagac cacctgaata
     4921 tttatcacca tactcataaa actgcccgag agtgaaccat atccct
//
LOCUS       DAOSNB010000195         4957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17112_length_4957_cov_5.578539, whole genome shotgun sequence.
ACCESSION   DAOSNB010000195 DAOSNB010000000
VERSION     DAOSNB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17112_length_4957_cov_5.578539"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLG42_08130"
     CDS             <1..237
                     /locus_tag="PLG42_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOU31214.1"
                     /translation="INITGGETMVFFGDGADKCSHFFTHERQIYISNITASASFMHIP
                     AMEALKERRFENTAYFEPFYLKDFITTKPKRKVI"
     gene            264..1043
                     /locus_tag="PLG42_08135"
     CDS             264..1043
                     /locus_tag="PLG42_08135"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOU31215.1"
                     /translation="MKSNVLILFVLMLTVFSLSGQDSPYKAGEQVTYIIHYGPINGGM
                     ATMTLTKEEYEGKEVLHSYLLGYTTGVADAVFKVRDIYESYFSPETGLPYKSVRNIQE
                     GRYRKYNVVIFDHETRSDSTILLSDLTGKHVTQKGIHDILSCFYYFRKNYLAKNYPFK
                     KGEAITINTWFADEFYPIILRFKGVEEVRTRMGKIKCYKFNPVTEVGRLFKTNEDVSI
                     WFSADKNCLPVKIRFDIFVGAFTAEINSFENLIEPLQFINR"
     gene            1070..1636
                     /gene="efp"
                     /locus_tag="PLG42_08140"
     CDS             1070..1636
                     /gene="efp"
                     /locus_tag="PLG42_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOU31216.1"
                     /translation="MATTADFRNGLVIEFNNDLYTIVQFQHVKPGKGPAFVRTKLKNI
                     RTGRVIDNTFTAGVKINVTRVERRPYQYLYKDEDYYYFMHSETFEQVHVPEHMIEQHE
                     FLKEGQNVEIVVHADTENILSVELPPFVVLEVTYTEPGLKGDTATNAMKPATVETGAT
                     VMVPLFINTGDKIKIDTRDGSYVERVKI"
     gene            1645..2877
                     /locus_tag="PLG42_08145"
     CDS             1645..2877
                     /locus_tag="PLG42_08145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOU31217.1"
                     /translation="MEDSSASKERLKISKFKLNALLDITLSINANLPVSELLAKYESI
                     LRNNLGIGKIMIFKYEEKWECILNAGFTEEYEKSIDVEKQLLPISETAIVTNTPGFEG
                     VDIIIPVFNNLVPLAYVFIGDIEEEGEGMSPVLKHLNFIQTISSIIIVAIENIRLFKE
                     SLRQAALKKELELASKMQMMLIPQEGKLPSKGKIKVCGFYFPHYEVGGDYYDCVSLSE
                     NQTGLCIADVSGKGISAAILMSNFQASLRALFTSQVDLTELIKKLNGIVVENAAGEKF
                     ITFFVARFNHDTGILEYINAAHNPPVFYDTVTGEIRHLEACCVGIGMLDEIPSVTRRE
                     IKIINHSKIVCYTDGLSELKDDKGKDIGTSMIIKHITNARTVKENIQEMLKELGIPEN
                     NPGLFDDVSIIAADLFCS"
     gene            complement(2905..4197)
                     /gene="tyrS"
                     /locus_tag="PLG42_08150"
     CDS             complement(2905..4197)
                     /gene="tyrS"
                     /locus_tag="PLG42_08150"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOU31218.1"
                     /translation="MNFVEELRWRGMIHDIMPGTEELLSREKVTGYIGFDPTADSLHI
                     GHMVQVMLLVHFQRAGHTPIALIGGATGMIGDPSGKSEERVLLDEDTLRRNQECIKKQ
                     LEKFLDFTSERPNRAIMVNNYDWMKDYSFLGFIRDIGKHITVNYMMSKDSVKKRLSPD
                     SKEGMSFTEFSYQLVQGTDFLHLYKTYNCKLQMGGSDQWGNIVTGTELIRRKTGGEAY
                     ALTCPLITKSDGTKFGKTEAGNVWLDREKTSPYQFYQFWLNVSDEDAARYIKIFTTLD
                     RETIENLISEHNQKPEERLLQKRLAEEVTVMVHSREDYNSAVEASQILFGRGTTESLK
                     KMDEKTFLTVFEGVPVFEVNKNLFCEGISLLELCTVHSQVFSSKGELRRLAQGGGVSI
                     NKEKVNDVEMKINTEFLLNGKYLLVQKGRKNYYIIRAV"
     gene            complement(4234..4770)
                     /locus_tag="PLG42_08155"
     CDS             complement(4234..4770)
                     /locus_tag="PLG42_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOU31219.1"
                     /translation="MNFIDILILIILGIAAIKGFIDGFIKELASLAALILGIWVAIRF
                     SSFTAAKLYDYFDMTGKYTGIIAFILTFLIVVIAIHFIGLLIDKLVKAVALGFINRLL
                     GLVFGVFKSTLILSVIFVILNTIDEHRPFLPKEKINQSMLYNPISDLAPALFPIIGEG
                     AFSKSFDRFKKKPDEVVM"
BASE COUNT         1580 a          852 c          991 g         1534 t
ORIGIN      
        1 ataaatataa caggtggcga aaccatggtg tttttcggag atggcgctga taaatgttcg
       61 cattttttta cacatgaacg gcaaatttat atcagtaata ttactgcttc ggcatcattt
      121 atgcatatac cggcaatgga agctttaaaa gagagaagat ttgagaatac agcttatttt
      181 gaaccatttt atttaaaaga ttttattacc acaaaaccca aaagaaaagt tatataaaac
      241 agcaagagag atagttaaag gaaatgaaga gtaatgtatt gatattattt gtcttaatgc
      301 tgacagtttt ttctctctca ggccaggatt ctccttacaa agcaggggaa caggttacat
      361 atattataca ttatggacct ataaatggtg gaatggcaac aatgaccctg actaaagagg
      421 aatacgaggg taaagaagta ttgcattcat atttattagg ttatacgacc ggtgtggctg
      481 atgctgtttt taaagtaaga gatatctatg agagctattt ttctcccgaa actggtttgc
      541 cttataagtc agtaaggaat attcaggaag gaaggtatag aaaatacaat gtggtgattt
      601 ttgaccacga aacaagatct gattcaacaa ttcttttgag tgatttaacc gggaaacatg
      661 taacccaaaa agggattcac gatatactat cctgttttta ttatttcaga aagaactatc
      721 tggcaaagaa ttaccctttc aaaaagggtg aggctattac tataaacaca tggtttgcag
      781 acgaatttta cccaataatt ctgagattta aaggagtgga ggaagtaaga acaagaatgg
      841 ggaagatcaa atgctacaaa ttcaatccgg taaccgaagt gggcagattg tttaagacaa
      901 atgaagatgt ttcaatctgg ttttctgctg ataaaaattg tttacctgtt aaaatacgat
      961 ttgatatatt tgtaggtgca tttacagcag aaataaatag ttttgaaaat cttattgaac
     1021 cattgcaatt cattaacaga taataaaata taaaagaaga gaagttttta tggctactac
     1081 agcggatttc agaaatggtc ttgtaataga gtttaacaac gacctttaca ctattgtaca
     1141 gtttcagcat gtaaaaccgg gaaaaggacc tgcttttgtt aggacgaagc taaaaaatat
     1201 aagaaccggc agggtgattg ataatacttt tactgcaggt gtgaagatta atgtaaccag
     1261 agtcgagcgt cgtccttacc agtatctgta taaagatgaa gactattact atttcatgca
     1321 ttctgaaaca tttgagcagg tgcatgtccc ggaacatatg attgagcagc acgaatttct
     1381 gaaagaagga cagaatgtgg aaattgtggt acatgctgat acagaaaaca tattatcagt
     1441 tgaacttcct ccttttgtag tactggaggt tacctatacg gaacccgggc tgaagggaga
     1501 tacagccacc aatgcaatga agccggcaac agttgagaca ggtgccacag taatggttcc
     1561 attattcatt aatacaggcg acaaaataaa aattgataca cgcgacggtt catacgtcga
     1621 aagagttaaa atctgatcct tcaaatggaa gacagcagtg cttcgaaaga gaggcttaaa
     1681 atctcgaagt tcaagctaaa tgcattactc gatataacat tgtcgattaa tgcaaatcta
     1741 cctgtttcag agctgctggc caagtatgaa tctatcctca gaaataatct gggtataggt
     1801 aaaattatga tttttaaata tgaagagaaa tgggaatgta ttcttaatgc cggttttaca
     1861 gaggaatatg aaaaatccat agacgttgaa aaacagcttt taccgataag tgaaactgct
     1921 attgtgacca atacccctgg atttgaaggg gttgatataa ttattccggt ttttaataat
     1981 cttgttcctc ttgcatatgt ttttattggc gatatagagg aagagggaga aggcatgagt
     2041 ccggttctca agcacctgaa ctttattcag acgatttcaa gtattataat tgttgctata
     2101 gaaaatatca ggctttttaa ggagagtctt cgtcaggcag cgttgaagaa ggagcttgaa
     2161 ctggcatcaa aaatgcaaat gatgcttatt cctcaagaag gtaagcttcc atcaaaaggt
     2221 aagataaaag tatgcggatt ttattttcca cattatgaag taggaggaga ttattacgat
     2281 tgtgtgagcc tgtcggagaa tcaaacgggc ctttgtatag ctgatgtttc gggcaaggga
     2341 atctctgctg ctatattaat gtcaaatttt caggcaagtt taagggcact ttttaccagc
     2401 caggttgacc tgacagagct tattaaaaag cttaatggca tagtagttga aaatgctgca
     2461 ggggagaaat ttataacttt ctttgttgcc agatttaacc atgacacggg aatacttgaa
     2521 tatatcaatg ctgcacataa tccacctgta ttctatgata cggttacggg tgaaataagg
     2581 catcttgaag cttgctgtgt tggcattgga atgcttgatg aaataccttc tgtaaccagg
     2641 cgggaaataa aaataattaa tcattcaaaa attgtctgtt atactgatgg actttctgaa
     2701 ctaaaagatg ataaggggaa agatattgga acttcgatga taatcaaaca tattacaaat
     2761 gcccgaaccg tcaaggaaaa cattcaggaa atgcttaaag agcttggtat tcctgagaat
     2821 aatcctggtc tcttcgatga tgtttcaata atagctgctg accttttttg ctcttgagat
     2881 aaaaaagaag tttactgaaa tccttcagac agcacgaata atataatagt tttttctgcc
     2941 cttctgcaca aggagatatt tgccgttaag tagaaattct gtatttattt tcatttctac
     3001 atcattaact ttttctttgt taatgcttac acctcctccc tgtgccagcc ggcgcagctc
     3061 accctttgaa gagaaaacct gcgaatggac agtgcataat tccagtaggg atatcccttc
     3121 gcagaataga tttttattta cttcaaagac tggtacacct tcaaatactg taaggaaggt
     3181 cttttcgtcc atttttttca gcgattcggt tgttcccctg ccaaagagaa tttgcgatgc
     3241 ctcaacagca gagttgtagt cttcgcgcga atgtaccatc acagtaacct cttcagccag
     3301 tcgtttctgt agcagtcgtt cctcgggctt ctggttatgt tcggaaataa ggttttcaat
     3361 agtttccctg tcgagagtgg tgaagatctt aatatatctg gcagcatcct cgtccgaaac
     3421 attgagccag aactggtaga actggtatgg agatgttttt tccctgtcga gccatacatt
     3481 tccggcttcg gttttaccga attttgtgcc gtctgatttt gttataagag gacaggtcag
     3541 agcatatgct tcgccgcctg tttttcttct tataagttca gtgcctgtaa caatgtttcc
     3601 ccactggtct gatcccccca tctgaagttt gcagttatat gttttgtaca ggtgtagaaa
     3661 atctgtcccc tgaacgagct ggtatgagaa ttcggtaaaa gacatccctt cctttgaatc
     3721 agggctaagt cgttttttta ccgagtcttt tgacatcatg taattgacag taatgtgttt
     3781 tccgatgtct cttatgaaac caagaaagga ataatccttc atccagtcgt agttatttac
     3841 cattattgcc ctgttgggtc tttcggatgt gaaatcaaga aatttttcga gctgtttttt
     3901 tatacattcc tggttcctgc gaagggtatc ttcatcgagc agaacccgtt cttctgattt
     3961 tcctgatggg tctcctatca ttccagtagc gcctcctata agggcaatag gtgtatgacc
     4021 ggccctctgg aaatgtacca ggagcataac ctgaaccata tgaccaatat gaagagaatc
     4081 agcagtagga tcaaacccga tgtatcctgt aactttttcc ctgctgagga gttcctctgt
     4141 tcctggcatg atgtcgtgaa tcatgcctct ccatctcagt tcttcaacga aattcataaa
     4201 tctaagtttc gaaccgcaaa tatagtaaat cagttacatt actacctcat caggtttctt
     4261 tttaaagcgg tcaaaactct tcgaaaaggc accttcgcca attattggaa agagtgcagg
     4321 tgctaaatca gaaatagggt tgtagagcat tgactggttt attttttctt tcggcagaaa
     4381 cggacgatgt tcgtcaatgg tattaagaat tacaaatatc acactcagta tgagtgttga
     4441 tttgaaaacg ccgaaaacta atcccagcag tctgtttata aagcccagtg caacagcttt
     4501 aacaagtttg tctattaaca aacctataaa atgaatagct ataactacaa ttaaaaaagt
     4561 gagtataaag gctattattc ctgtatattt tcccgtcatg tcgaaataat cgtagagttt
     4621 tgctgctgtg aaacttgaaa atcttattgc aacccatatt cctagaatta atgcagcaag
     4681 agaagcaagt tccttaataa aaccatcaat gaatcctttt attgccgcaa tgcctaatat
     4741 tattaagatc agtatgtcga taaaattcat agcttattgt tcaaaaaggt gaaagtataa
     4801 aagattgcaa gattttcaaa actcttgaaa aaagaaatat ttcataataa ataatggtct
     4861 gactgaattt cttacataag gattcatatt gatttttatt tatttgatta tgtaacattg
     4921 tttctgtaaa attaaaaaag ccacggctga aaaaaat
//
LOCUS       DAOSNB010000196         4930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17234_length_4930_cov_4.761641, whole genome shotgun sequence.
ACCESSION   DAOSNB010000196 DAOSNB010000000
VERSION     DAOSNB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17234_length_4930_cov_4.761641"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..675)
                     /gene="coaBC"
                     /locus_tag="PLG42_08160"
                     /pseudo
     CDS             complement(1..675)
                     /gene="coaBC"
                     /locus_tag="PLG42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860700.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            complement(682..1020)
                     /locus_tag="PLG42_08165"
     CDS             complement(682..1020)
                     /locus_tag="PLG42_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOU31220.1"
                     /translation="MDYKKINAPTTTTTRNIEKYLDATGNIYEAVVIIGKRANQIAVE
                     MKQELNKKLEEFSSFTDNLEEVFENREQIEISKFYEKLPKPTLIAMQEFEEGKIYYRN
                     PEKASKTKSE"
     gene            complement(1041..1841)
                     /gene="bamD"
                     /locus_tag="PLG42_08170"
     CDS             complement(1041..1841)
                     /gene="bamD"
                     /locus_tag="PLG42_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOU31221.1"
                     /translation="MRFRFLIYLIISTVLVSCGEYEKLLKSTDYELKLTKLKEYYEQG
                     KYAKASELINQVIPVYRATEEAEKLTWINAQCYLNMKDYLMAGVEFKNYADMYAYGPH
                     IEEAVFMEAMCKYYLSPRPELDQQYTREALEGFQIFIGRFPYSTKVPDAQRYIDELNE
                     KLARKAYISAKLYYDMKQYKAAVVSLENCINKYPGSKYREEMMFLKLNSLYLYAENSV
                     PSKQIERYQSTLDDYFSFIEEYPKTIHYKEVATIFQNVNKKLKTKIAD"
     gene            2163..>3343
                     /locus_tag="PLG42_08175"
     CDS             2163..>3343
                     /locus_tag="PLG42_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOU31222.1"
                     /translation="MDIFEKIQKNKGALGQYSDVGDGYFMFPKLEGEIAPRMKFRGKE
                     VLTWSLNNYLGLANNPEVRKADAEYAAQYGLAYPMGARMMSGQTAVHEKFEQMAAEFV
                     HKESAYLLNYGYQGMVSIIDALVDRHDVIVYDSESHACIMDGLRLHFGKRFVYPHNDI
                     DSCEKQLQRATKIVEQTGGGILVITEGVFGMAGDLGKLDKIAALKKKYNFRFLVDDAH
                     GFGTMGPTGAGTGEHFGVQDEIDVYFATFAKSMAGIGAFVASTREVITYLRFNMRSQI
                     YAKALPIAMALGAIKRLELIKAHPEYREKLWTIVRALQKGLREAGLDLGRTESPVTPV
                     FMKCPLTQVSQLIYDLRENYNIFCSVVIYPVVPRDVVMLRLIPTAAHTLEDVEYTVNA
                     F"
     assembly_gap    3344..3443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3536..>4930
                     /locus_tag="PLG42_08180"
     CDS             3536..>4930
                     /locus_tag="PLG42_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOU31223.1"
                     /translation="MKWSAILGSSILGRNSVFKEIFTPYNSEQPQDNESKIIRTGCPA
                     HNCGGRCLLKVHVKDGIITRIETDDRPGDDMSDPQLRACIRGRSYRKRQYHPDRLKYP
                     LKRTGKRGEGKFERITWDEAYEIMYNEISRVIKQYGNSAIFIPYGTGSYTNLNGRWPA
                     VRLMNLLGGSLGYYNSYSWACTNIATPYVYGTLNTGNQRQDWLNSRYIIMWGWNPAEM
                     KDGTNSEYFIRRARENGARTVCIDPRLSMSAIALADEWIPIRPGTDAAMMSAMAYVII
                     TEKLTDNDFIRRCCSGYDSTQMPAGCENEESYKDYILGTRDGIPKTPEWAEKITAVPA
                     ATIARIAREYATIKPAVLYQGYGMQRRAYGEQTVRAGAALSAITGNIGIPGGWASGLG
                     SQAGGEPFWSVFPSGENPVKARIPVYLWTEAIIRGKEMGPADGVRGVAKLDNNIKLIW
                     CIASNILINQHSNIN"
BASE COUNT         1373 a         1011 c         1078 g         1368 t
ORIGIN      
        1 gcggctgcat cggccagggc aatgcccatt tttccggtag aataattgct aatataccgt
       61 accggatcga tgggttccct ggtgggaccc gcatttacca gtatccgttt accggcgagg
      121 ggtttaagtt ttttgtgtga agaatttttc aatctccctt acaatttctt cgggttcaac
      181 catccggcct ttaccgtaaa gtccgcttgc aagttctccg gtttcagggt cgagtattat
      241 attgccaaac gaacgcagaa tttctatgtt tctggctgtt gccggatgtt tgtacatttc
      301 tgaatccatt gaaggggcaa caaatacagg gcatcttgca gaaaggtatg tggtaagaag
      361 gagattgtct gcgatgccac ctgccatttt tgctattgta tttgctgttg caggagcaat
      421 aataaaagca tcggcccatt gccctaagtc aacatgactg ttccagtttc cgttttcagg
      481 atcaaaaaaa ccggaaaaga ccgggttacc tgaaagcgtg gcaagggtaa gcggagtgat
      541 aaattcctta gcatggtcag tcataaccac gcgtacctgc gcacctgctt taaccagcag
      601 ccttatcaga gaagctgttt tgtaagcggc aatcccgccg gttacaccta ccagtatttt
      661 tctgccggcg agcattcagc tctattcact ctttgttttt gatgcctttt caggattcct
      721 gtaatatatc ttcccttctt caaattcctg cattgctata agcgtaggtt tgggaagctt
      781 ttcataaaac ttcgatattt caatctgttc cctgttttca aaaacttctt caagattatc
      841 ggtaaacgac gagaactctt cgagtttctt attaagttcc tgcttcatct ccaccgcaat
      901 ctggttagct ctcttgccaa taattacaac tgcctcgtaa atattgccgg tggcatcaag
      961 atacttctca atattacgtg ttgtagttgt tgtgggagca ttaatcttct tgtagtccat
     1021 atcctgttgt taaattttat tcagtcagca atttttgttt taagtttttt attaacattc
     1081 tggaatattg ttgcaacttc tttataatga attgtttttg gatattcctc aataaaggag
     1141 aaatagtcgt cgagtgtact ctggtaacgc tcaatctgtt ttgaaggaac actgttttct
     1201 gcatacaggt aaagtgaatt cagcttcaga aacatcattt cctccctgta ttttgagccg
     1261 ggatatttgt ttatacaatt ctcgagtgag actactgcag ccttgtactg cttcatgtcg
     1321 taatagagtt tggcacttat ataagccttt cttgcaagtt tttcgttaag ttcatcgata
     1381 tacctctggg catccggcac tttggtgcta tatggaaatc tcccgataaa gatctgaaag
     1441 ccttcgagtg cttcacgtgt atattgctgg tcgagttccg gcctgggtga gagataatat
     1501 ttgcacattg cctccataaa cacagcttct tcaatatgag gaccatacgc gtacatgtca
     1561 gcataattct tgaactccac tcctgccata agataatcct tcatgttcag ataacactgg
     1621 gcgttaatcc atgtgagttt ctcggcctct tcagttgcac ggtaaacagg gatcacctgg
     1681 ttaattaact ccgaagcctt cgcatactta ccctgctcgt aatactcctt gagctttgta
     1741 agcttcaact cataatcagt acttttaaga agcttttcat actctccgca ggagacaagc
     1801 acagtactaa taataagata tatcagaaac cggaatctca tatctaaaaa taatgtgcaa
     1861 agatactgaa tattttttta atatgacaaa tcaacgtaaa tgccagttta ttattatggc
     1921 tgttcataat atattcagtt attgattcat ttattcatac aaacgttcat ctctaacgaa
     1981 gtcttgtgag agcatattct ctctgcaata attattgatg tataaaaggc tgtatttatg
     2041 attattatgt ttcattcccg tgttaatgtc aataaattag gtataaaatc gtagaatgta
     2101 atgagttaat atctttattt ttgccttagc tttacggatt acaaaattaa catcaaaaca
     2161 ttatggatat tttcgaaaag atacagaaga acaaaggcgc attaggacaa tattccgacg
     2221 ttggcgacgg gtactttatg tttccaaaac ttgaaggtga aattgctccg aggatgaaat
     2281 tccggggcaa agaagtcctc acatggagtc ttaataatta cctgggtctg gccaacaacc
     2341 ctgaagtcag gaaggcagat gctgaatatg ctgcacaata tggactagca tatcctatgg
     2401 gtgcaaggat gatgtcgggc cagacagctg ttcacgagaa atttgagcag atggcagctg
     2461 aatttgttca taaggaatca gcttacctgc ttaattacgg gtatcaggga atggtctcca
     2521 taattgatgc gctggtcgac aggcacgatg taatagtata tgattcagaa tcgcatgcat
     2581 gcataatgga cggattgagg ctccatttcg gaaaaagatt tgtttacccc cataacgata
     2641 ttgacagttg cgaaaagcaa cttcagcgtg ctacaaagat tgtggagcag accggcggtg
     2701 gtatacttgt tataaccgaa ggcgttttcg gtatggcagg tgaccttgga aaactcgata
     2761 aaatagctgc cctcaagaaa aaatacaact tccgctttct tgttgatgat gctcatggtt
     2821 ttggtactat ggggccaact ggtgcgggca ccggagaaca tttcggtgtg caggatgaga
     2881 ttgacgtata tttcgcaaca tttgcaaaat ctatggctgg aataggcgcc tttgttgcca
     2941 gtaccaggga agtgatcaca tatctgaggt tcaacatgcg ctcccagata tatgccaaag
     3001 cccttcctat tgctatggct ctgggtgcaa taaagaggct tgaactcatc aaggcacatc
     3061 cggaatacag ggaaaaactc tggactattg tgcgtgcact tcagaaagga ctcagggaag
     3121 caggactcga cctcggacgt accgaatcac ctgttacacc ggtcttcatg aaatgccccc
     3181 ttacccaggt ttcacagctg atttacgacc tgagagaaaa ctataacata ttctgttcag
     3241 tggtaattta tccggtggtt ccacgcgatg tagtgatgct caggcttatt cctacagcag
     3301 cccatactct tgaagatgtc gaatacacgg taaacgcttt cagnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnagttttg cctggaaaag gcagaaggaa gctgttaatt
     3481 tcagataaga ataatgggtt cagatggtat cttttatacc aacaggagat cattcatgaa
     3541 atggtcagca atccttggct cttcaattct tgggcgcaac agtgtgttta aggaaatatt
     3601 caccccatat aactctgaac agccgcagga taatgaatca aaaataatcc gtaccggatg
     3661 ccctgcccac aactgcggag gacgttgcct gctgaaggtt cacgtcaaag acggcataat
     3721 aacccgtatc gaaaccgacg accggccagg tgatgatatg tctgatcctc aattacgggc
     3781 atgtataagg ggacgttcat accgtaaaag gcagtatcac ccggatcgtc tgaaatatcc
     3841 tctcaaaaga acagggaaaa gaggtgaagg taagttcgaa agaataacat gggatgaggc
     3901 atacgaaata atgtataatg agatttcacg cgtcataaaa caatatggca attctgcaat
     3961 ttttatccct tatggaacag gctcatatac aaatctgaac ggtcgctggc ctgcggtaag
     4021 actgatgaat ctccttgggg gtagtctcgg ttattacaac agttacagct gggcatgcac
     4081 aaatattgca actccttacg tttacggcac actcaacacc ggcaatcagc ggcaggactg
     4141 gctcaattcc agatatataa tcatgtgggg ctggaatcct gccgagatga aagacggaac
     4201 caacagtgag tatttcatcc gacgggcaag ggagaacgga gccaggactg tttgcattga
     4261 tccgcgactg agcatgagtg ccatagcact ggccgatgaa tggataccaa tacgtccggg
     4321 caccgatgca gcaatgatga gtgctatggc ttatgtaata atcactgaaa aacttactga
     4381 caatgatttt ataaggaggt gctgttctgg ctacgacagc acccagatgc ctgcaggctg
     4441 cgaaaatgaa gagtcgtaca aagattacat cctcggcacc cgtgacggga ttcccaagac
     4501 gccggaatgg gcagaaaaga taacagctgt tccagcagcc acaattgcaa gaattgcaag
     4561 ggagtatgcc acaattaagc cggcagtgct ttatcagggt tatggcatgc agagaagagc
     4621 ttatggtgag cagactgtaa gggcaggcgc tgctctttca gcaataaccg gcaacatcgg
     4681 gatacccgga ggatgggcaa gcggacttgg cagccaggcc ggtggagaac ctttctggag
     4741 tgtattcccg tcaggagaaa atcctgtaaa ggcaagaata ccggtttatc tctggacaga
     4801 agctattatc agaggcaaag agatgggacc cgccgatggt gttagaggtg ttgccaaact
     4861 cgataacaac attaaactta tatggtgcat tgcttcaaat attctcatca accagcatag
     4921 caacattaac
//
LOCUS       DAOSNB010000197         4834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17628_length_4834_cov_5.376229, whole genome shotgun sequence.
ACCESSION   DAOSNB010000197 DAOSNB010000000
VERSION     DAOSNB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17628_length_4834_cov_5.376229"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="dxr"
                     /locus_tag="PLG42_08185"
     CDS             <1..748
                     /gene="dxr"
                     /locus_tag="PLG42_08185"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOU31224.1"
                     /translation="SKTGSKIVPIDSEHSAIFQCLEGENKKSVEKIVLTASGGPFLGY
                     SYEQLQNVTAAEALKHPNWDMGDKITIDSATLMNKGLEAIEAKWLFNLPPEKIEVVIH
                     PQSVIHSLVYFYDGSVKAQMGVPDMRIPILYALSYPERLQSDLPRLDLYKYENLTFKE
                     PDRKIFRNLPLAFEALKTGGNMPCILNAANEVAVHGFLEGKTGFLQMPDVVEHAMSKT
                     PFVADITLEILEASDYESRERAKDYLNQKF"
     gene            753..2078
                     /gene="rseP"
                     /locus_tag="PLG42_08190"
     CDS             753..2078
                     /gene="rseP"
                     /locus_tag="PLG42_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOU31225.1"
                     /translation="MTILIKILQFVLSLSILVIIHELGHFIFAKFFKTRVEKFYLFFN
                     PWFSIFKFKKGETEYGLGWLPLGGYVKISGMIDESLDREQLKQPPQPWEFRAKPAWQR
                     LLIMTGGVLFNFLLAFFIYVSILYAWGETYLPTSNVKYGIVTDSVGYSIGLRNGDKIL
                     SVDNQYIDNFYQISKEIILNNRSTIQVEREGKVINIQIPKEYIRKAIKGGGHIDARIP
                     FSPFIVSGFGKTSPAKEAGVMVGDEIIGADSIKFSFFDEFQNYLLKNKDRIITLNILR
                     NGENINIPVKPTPAGRLEVARTYENIFEYKTIKYSFIESIPAGIKLGITTIGDYLKQF
                     KLIFSKEAKGYESLGGFITIGRIFPEVWNWHAFWNLTAFLSIILAIMNILPIPALDGG
                     HVLFLTYEVVTGRKPSDKFLEYAQITGMIILFALLIYANGNDIIKLIRK"
     gene            2144..2320
                     /gene="tatA"
                     /locus_tag="PLG42_08195"
     CDS             2144..2320
                     /gene="tatA"
                     /locus_tag="PLG42_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOU31226.1"
                     /translation="MGKIGPLEIVLILLIVVLLFGGRKIPELMKGLGQGLKEFKKASK
                     YDPEEEKKEQVNKD"
     gene            complement(2428..3519)
                     /gene="mqnC"
                     /locus_tag="PLG42_08200"
     CDS             complement(2428..3519)
                     /gene="mqnC"
                     /locus_tag="PLG42_08200"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOU31227.1"
                     /translation="MQNLESLYRKALVLEPLTFEESVMLYNEAPLSMLVFIADEIRKQ
                     LHPGNRVGWIIDRNINITNICFSQCLFCNFCRRKDSADAYVTTMDEYRQKINELFSLG
                     GDQILLQGGMNPDLDLSFYKNLFISLKKEFPSLKLHALGPPEIVYLSKKEKMTYAEVL
                     KELIDSGLDSLPGAGAEILSERVRRIVSPAKAKVVEWLDVMREAHRLNLPTSSTMMAG
                     HIETREERIMHLIHLRQVQSEKPEGTFGFITFVPWPFQDKNTRLAKEMGIKNNINATE
                     YIRLVAISRIVLNNIPNIQASLLTVGKETAMLSLHAGANDLGSVMIEENVVSAAGNTN
                     RFNAADIQEIIREAGFVPVRRNQKYEPLE"
     gene            3647..>4834
                     /locus_tag="PLG42_08205"
     CDS             3647..>4834
                     /locus_tag="PLG42_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOU31228.1"
                     /translation="MKIQIRAVSQISPDQSVICIVGKDEIPSHIVLNRSEKEYALRRL
                     SENDEYVFVNSYYKCTYFVKANTELPHFMMLEEIRKRASSMKQLIRANNHTEIVITSE
                     GLSEDVSEAFAEGFILSIYSFRKYKTKEEEKEKGRYPQKILLHNIKNKDKVLWLSALC
                     EAVYMVRDLINEPPNFLNAPALAREIKKIANSAGFTVEVLNKGKIEALKMGGLLAVNR
                     GSIDPPVFCILEWEPEGKVNNRPLVLVGKGVIYDTGGLNIKTGENMSQMKADMAGAAT
                     VAGIMYVMAKCNIPLHVIGLVPATDNRPGGNAYLQGDIITMFNGTTVEIGNTDAEGRL
                     ILADALSYAGKYKPELIIDIATLTGSAAMTFGNKAIAVMGNADEKYFKLLEEAGNMVY
                     ERVA"
BASE COUNT         1515 a          886 c         1010 g         1423 t
ORIGIN      
        1 atcaaaaacg ggaagcaaga tagtaccaat agattctgaa cattcggcta ttttccagtg
       61 ccttgagggt gaaaataaaa agtcggttga aaagatagtt ttgactgctt cgggaggtcc
      121 ctttcttggc tattcctatg aacagcttca gaatgttaca gcagctgaag cactgaaaca
      181 cccaaactgg gatatggggg ataaaattac aattgattca gccacattaa tgaataaagg
      241 acttgaggct attgaagcaa aatggctttt taacctgccc cctgagaaga ttgaggttgt
      301 tatacatcct cagtctgtaa ttcattcact tgtttatttc tatgatggtt cggtgaaagc
      361 ccagatgggg gtgcctgaca tgagaatccc aatactttat gcactcagct atccggaacg
      421 cctgcagtcg gaccttcccc gccttgacct ttacaaatat gaaaacctta cttttaagga
      481 acccgatagg aaaattttcc gtaacctgcc gcttgctttt gaagctctga aaacaggcgg
      541 aaacatgcca tgcatactca atgctgccaa tgaagtggca gtacacgggt tcctcgaagg
      601 aaaaacaggc ttccttcaga tgcccgatgt ggttgaacat gcaatgtcaa aaactccttt
      661 tgtggcagat ataaccctgg aaatactcga ggcttcagat tatgaaagca gggagagagc
      721 aaaagactat ttaaaccaaa aattttagaa aaatgacaat cttaattaag atactgcaat
      781 ttgttttaag tctgtcgatt ttagtaatta tccacgaatt agggcatttt atatttgcaa
      841 aattcttcaa gacacgtgtc gagaaattct atctgttctt taatccatgg ttttcaattt
      901 ttaaattcaa aaaaggagaa acggaatacg ggctgggctg gctgccactt gggggttatg
      961 ttaaaatttc agggatgatc gacgaatcgc tcgaccgtga gcaattgaaa caaccacctc
     1021 aaccatggga attcagagca aaaccagcct ggcagagatt gttgattatg acaggcggag
     1081 tacttttcaa tttcctgctg gcatttttta tatacgtatc tatactttac gcctggggcg
     1141 aaacttacct gccaacttca aacgtcaaat acggcatagt taccgatagc gttggttatt
     1201 caattggtct gcgtaacgga gataaaatcc tttctgtaga taatcagtat attgataatt
     1261 tttatcagat atccaaagag attattctca ataatagaag tacaatacag gttgagagag
     1321 aaggcaaagt aataaatatt cagattccca aagaatatat cagaaaagct attaaaggcg
     1381 gagggcatat tgatgccaga attcctttca gcccttttat agttagcgga tttggcaaaa
     1441 catcacctgc aaaggaggct ggtgttatgg tgggagatga aattataggt gctgatagta
     1501 taaaattctc attctttgat gaattccaga actaccttct taagaataag gaccgtatta
     1561 ttacacttaa catccttcgt aatggtgaga atatcaatat ccctgttaaa cctacacctg
     1621 ccggcaggct tgaagttgca agaacttacg aaaatatttt tgagtataaa accataaaat
     1681 atagttttat tgaatcaatc ccggccggaa ttaaactggg tattacaacc ataggagatt
     1741 acctcaagca gtttaaactc atattttcaa aagaagcaaa aggttatgag tcacttggag
     1801 gatttattac aatcggaaga atatttcctg aagtatggaa ctggcatgca ttctggaatc
     1861 ttactgcttt cctgtcgatt atccttgcta taatgaatat attacccatc ccggcactcg
     1921 atggcggtca tgtattgttt cttacatacg aagttgtgac aggaagaaag cccagtgata
     1981 agtttcttga atatgctcag ataactggaa tgataatact ttttgcattg ctgatatacg
     2041 ccaacggaaa cgatattata aaacttatca gaaaatagtg tgaataatag tttgttttta
     2101 ataaagtaat tgtaactttg taaggactta aaattttaca gaaatgggaa agattggacc
     2161 tttggagata gttttaatac ttcttattgt ggtacttctt ttcggaggcc ggaagattcc
     2221 tgaactgatg aaaggacttg gacagggtct gaaagagttc aagaaggctt caaagtatga
     2281 cccggaagaa gaaaagaaag aacaggttaa caaggattaa ttttccaggc aagatatacc
     2341 tgtagtaaac cgtaaaggtt aaggataagt ttttgagcaa tagaacattc ctgatgttct
     2401 atttttcttt tatctggcaa acaaaactta ttctagaggt tcatatttct gattccgcct
     2461 cacagggaca aaacctgctt cccttattat ttcctgaata tctgctgcat tgaacctgtt
     2521 agtatttcct gcggcactta caacgttttc ttctatcata acagagccaa ggtcgttggc
     2581 acctgcatga agcgataaca tagctgtctc tttgccaacg gtaagaagcg atgcctgaat
     2641 attcggtata ttgttcagaa caattctgct gatagccaca agcctgatat attctgttgc
     2701 attgatattg ttctttatgc ccatttcttt tgcaaggcgt gtgttcttat cctgaaacgg
     2761 ccatggaacg aatgtaatga aaccgaaggt gccctcaggt ttttctgact gcacctgacg
     2821 caggtgaatg agatgcataa tcctttcctc acgcgtctca atgtgtcctg ccatcattgt
     2881 tgaggaagtg ggtaaattaa gcctgtgtgc ttccctcatc acatcgagcc attccaccac
     2941 ttttgctttt gctggtgaaa caattcttct gactctctcc gatagaattt cggcacctgc
     3001 cccgggcaaa ctgtcaagcc ctgaatcaat gagctccttc aatacttcag cataggtcat
     3061 tttttctttc ttcgaaaggt aaactatttc cggcggacca agagcatgaa gtttgagtga
     3121 gggaaactcc tttttcagtg aaatgaaaag attcttataa aaagataaat cgagatcagg
     3181 attcatgccc ccctgcagca atatttgatc cccgccaaga ctgaataatt cgttgatttt
     3241 ctgtctgtat tcatccattg ttgtaacgta cgcatcggcc gaatctttcc tgcgacagaa
     3301 gttgcagaaa aggcattgag aaaagcatat gttggtaatg ttgatattcc ggtctattat
     3361 ccagcctacc ctgttgccag gatgaagctg tttcctgatt tcatcagcaa taaaaaccag
     3421 cattgaaaga ggcgcctcat tgtaaagcat aacactctct tcaaaggtca gcggctcaag
     3481 tacaagagct ttcctgtata acgattcaag attttgcact tcagtgaatt tcttattctt
     3541 tcaggttaaa gatttaaaat atggtctgta atgcaaaggt atatctttca gatttagcct
     3601 gtaaattttt tgattaactt tacgctctct ttcagaagtt taatttatga agattcaaat
     3661 aagagctgtt tctcaaatat ctcccgatca atcggttata tgtattgttg gtaaggacga
     3721 aataccttct catattgttc ttaaccggag cgaaaaagaa tatgcactca ggaggctcag
     3781 tgaaaatgat gaatatgtct ttgttaattc ttattacaag tgtacatatt ttgtaaaggc
     3841 gaatacagaa cttccgcact ttatgatgct ggaagagatc aggaaaagag cctcatcaat
     3901 gaagcagctt ataagggcga ataatcacac ggaaattgtt ataacttcag aaggattatc
     3961 agaagatgtc agtgaagctt tcgctgaagg attcatattg agtatttatt cattccgaaa
     4021 atacaagacg aaagaagaag aaaaggaaaa aggtagatat ccgcaaaaaa tattgcttca
     4081 taacataaag aataaagata aagttctgtg gttaagtgct ttatgtgagg cggtatatat
     4141 ggtgagggat cttatcaacg agcctccaaa ttttcttaat gcacctgcac ttgcaaggga
     4201 gataaagaag attgctaatt cagctggttt tacggtagaa gtgctaaata aaggtaagat
     4261 agaagcactt aaaatgggag gattactggc tgttaatcgt ggaagtattg accctccggt
     4321 attctgcatt cttgaatggg aacctgaagg caaagtaaat aaccggccgc tggttcttgt
     4381 tggcaaaggt gtcatttacg acacaggagg gctgaatatt aaaaccggtg aaaacatgtc
     4441 gcaaatgaaa gccgacatgg caggagctgc aacagttgca ggtattatgt atgtaatggc
     4501 aaagtgcaat attccgcttc atgtaattgg acttgtacct gccactgata accggccggg
     4561 aggtaatgcc tacctgcagg gcgatatcat aacaatgttc aatggtacaa cggttgaaat
     4621 cggaaatacc gatgctgaag ggaggcttat tcttgccgat gccttaagtt atgcaggaaa
     4681 atacaaacct gaactgataa ttgatattgc cacacttaca ggttctgctg ccatgacttt
     4741 cggaaacaag gcaatagctg taatgggaaa tgcagatgag aaatatttta aacttctgga
     4801 agaagctgga aatatggtat acgaaagggt ggca
//
LOCUS       DAOSNB010000198         4833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17635_length_4833_cov_5.699247, whole genome shotgun sequence.
ACCESSION   DAOSNB010000198 DAOSNB010000000
VERSION     DAOSNB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17635_length_4833_cov_5.699247"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLG42_08210"
     CDS             complement(<1..569)
                     /locus_tag="PLG42_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU31229.1"
                     /translation="MKRFLGFVKKEFIHIFRDYRTLIILFGIPAVQILVFGFVVSTDI
                     KDAGVAILDLSRDETTQKLTDKILSSGFFRKTGELLNYNDIDRIFKKGNTKAVIIFEK
                     DFGSRLFREGAASVNIIADGSEPNVATLVSSYISAIISDFNREMNKTLLSSSPVVQPE
                     VRMYFNPSLKSHFMFVPGVITLIMILICAL"
     gene            complement(566..1327)
                     /locus_tag="PLG42_08215"
                     /pseudo
     CDS             complement(566..1327)
                     /locus_tag="PLG42_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1387..2295)
                     /locus_tag="PLG42_08220"
     CDS             complement(1387..2295)
                     /locus_tag="PLG42_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU31230.1"
                     /translation="MKTVIEAEEISRNFGKVKALDNVTFSVNEGEIFGFIGPDGAGKT
                     TLFRIVTTLLLPDSGTMKVLGFDSIKDFRELRKHIGYMPGRFSLYPDLSVEENLNFYA
                     TVFGTTVNENYSLIEAIYKHLEPFRKRLARDLSGGMKQKLALSCALIHRPRLLVLDEP
                     TTGVDAVSRVEFWEILQGMKEHNITVLVSTPYMDEAMRCNRVALIQQGQILTIDTPDN
                     IKSGFRGKLFKIKAPGKFKLINAVRDHHNTLSAYPFGDSVHATFFNDTVPEDFVNYLT
                     MKGIENVQITESEAHIEDRFLELMNI"
     gene            complement(2319..3200)
                     /locus_tag="PLG42_08225"
     CDS             complement(2319..3200)
                     /locus_tag="PLG42_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU31231.1"
                     /translation="MNRIISLILPAALLIAGCSNNKSEADAYGNFEVTEVIVSAETNG
                     RILQCDIREGMEIEKGALIALIDTTLFHLQKAEIDAGIAGITTRISSINAQNAILEQQ
                     IENLQVNIKRIENMLKDDAATKKQYDDLTGQVEVLRKQITANNTQKASVQAEVAVYNA
                     KKATLEEQISRCMVRSPLKGTILQKYCETGEMAAAGKPLAKIADLSVMKLKVYVSGAQ
                     LDKIKTGQECTVRIDDGKKGYKTLSGKVTHISEKAEFTPKIIQTKEERVALVYAVTIE
                     VQNDGTIKSGMPGEAIF"
     gene            complement(3212..4510)
                     /locus_tag="PLG42_08230"
     CDS             complement(3212..4510)
                     /locus_tag="PLG42_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU31232.1"
                     /translation="MIQRICLTVIVSFVLILKGYPQKIITLSDCYDAAMKKSSLAGEQ
                     ELHTSIWQIKDKNLSKNWLPSLYAGASFIYNSEVIDLGEKFSTVPIPGLADAITPLPH
                     EQYRITVDITQTIYDGGATKAARELENTNRKVNEKQTETDLYKLRSLVNNYYFSILML
                     DRQKESFEGFREVIEKRIASMESALKNEIIVPSDIDIMKAEKIKIEQQLKEIEIKKTS
                     LLKILSDLTGIAVEPADQLAVPELPGETDIELMRPELQYFDIRKEQLQAGIKTIATRR
                     MPKAYGFATLGYGNPPGNNFLKDEFAPYYIIGAGIKWNIFDWSRVKNEKEILTLEQNI
                     IEKRKTDLEENLRRMLEAKKAEILSLESLLESDRELIELRKKISSAAISRYENGVITA
                     AEMLGELNAERQAAVTYELHKVSLAMAKAEYFNISGQEIK"
     gene            complement(4551..>4833)
                     /locus_tag="PLG42_08235"
     CDS             complement(4551..>4833)
                     /locus_tag="PLG42_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31233.1"
                     /translation="IINELNRKPERIQRLIRNIDFKLFYKTLSEQHGKEFEKYDITPD
                     SLLQIMTSIAAISIFPFIGKNFSKSLWRISTKISTNISKKEKNLHPILF"
BASE COUNT         1139 a         1002 c         1037 g         1655 t
ORIGIN      
        1 agggcacata tgagtatcat gataagtgtg attacaccgg gaacgaacat gaagtggctt
       61 ttcagggagg gattgaaata catgcgtact tcgggctgga ccacagggga tgagctgaga
      121 agtgttttat tcatttcacg gttgaagtcg gaaataattg ctgagatata acttgaaaca
      181 agagttgcca cgttaggctc agagccatca gcaataatgt ttacagaagc ggcgccttcc
      241 ctgaaaagcc tgcttccgaa atctttttca aatatgatta ctgcttttgt attgcctttt
      301 ttgaatatcc tgtctatgtc attataattg agaagttcgc cggtttttct gaagaagcct
      361 gaagagagga ttttatctgt aagtttctgc gttgtttcgt cccgggaaag gtcaagtatt
      421 gccacaccgg catcttttat atcagtactg acgacaaatc caaaaaccag aatctgtaca
      481 gcaggtatac cgaaaagtat tatcagggtg cggtaatccc tgaagatatg gatgaattcc
      541 ttttttacaa agccaaggaa gcgtttcatt tgtttttgtt taaaatatta attgaacttt
      601 tatcgggccg ggcaatgctg ataaatactt cttcaatgct ctccgcatta aattgttttt
      661 tgagattttc tggagtgtcg agtgccacaa ttcgtccttc gctcattatt gaaacgcggt
      721 cgcagtattc tgcttcgtcc atgtaatgtg tagtaacgaa gacggttatt ccactatcgg
      781 ctgtttcata aatcatttcc cagaactggc gccttgtgat ggggtctaca ccgccggtag
      841 gttcatcgag aaaaactatt tccggttggt gcagaactgc cactgcaaac gcgagtttct
      901 gtcggaagcc aaggggaagg ccggatatca gccggtcctt atactcagtg aatctgagtt
      961 tttccagaag ggcttctgtt ctttctttta tcaattcgtc actcattccg taaatgccgc
     1021 cgtacagctg tatgttttct tttacggtaa ggtcttcgta caaagaaaat ttctggcaca
     1081 tatagcctat atgtttcttt atcatctcag attctttcca cgcatcgaag ccggcaaccc
     1141 tgacttcacc cgaagtgggt tgaagcagac ctgaaaggat tctgatagcg gtagttttac
     1201 ctgcaccatt agctcccaga aagccaaata tctcacctct gtaaacctca aaagtcagat
     1261 tgtcattggc aacgaagctg ccgaattttt taacaagatt tctgactgta ataactgttt
     1321 cacctctttc catggtctct tctccttttg aggaaagata accagttacc ggtttactgt
     1381 tgtctttcat atgttcataa gttcaagaaa gcgatcttcg atatgtgctt cgctttcagt
     1441 tatttgcaca ttttctattc ctttcattgt caggtaattg acaaaatctt caggaacagt
     1501 atcgttaaaa aatgttgcat ggactgaatc gccgaagggg tatgcagaaa gggtgttgtg
     1561 gtgatctctc acagcgttaa taagtttgaa tttgccggga gcttttattt tgaacagttt
     1621 gcccctgaag ccactcttta tgttatccgg cgtgtcgata gtgagtatct gtccctgctg
     1681 tattaatgcc accctgttgc atctcattgc ttcgtccatg taaggtgttg atacgagaac
     1741 cgttatattg tgttctttca taccctgaag gatctcccag aattctaccc ttgatacagc
     1801 gtcaacacct gttgtgggtt cgtcgagtac aagcagacgg ggccggtgga ttaatgcgca
     1861 cgaaagtgcc agtttctgtt tcatgcctcc cgacaggtcc cttgccagcc ttttcctgaa
     1921 aggctccaga tgtttatata tggcttcaat caggctgtag ttttcgttaa cggtggtgcc
     1981 gaaaacagta gcataaaaat taaggttttc ctcaaccgac agatcaggat aaaggctgaa
     2041 acgtccgggc atataaccaa tatgtttgcg caactccctg aaatctttta tagaatcgaa
     2101 tccaagcact ttcatggtgc cgctgtcggg caggagcagg gtggttacaa tgcggaagag
     2161 agttgtcttg cctgccccgt cgggaccaat aaaaccgaat atctcaccct cgttaacaga
     2221 gaacgttaca ttgtcgagag ctttaacctt cccgaagttc ctggaaattt cttctgcttc
     2281 aattacggtt ttcatggttc agtaaaaaag cctgtgtatt aaaaaatagc ttcacctggc
     2341 atacccgatt ttatcgttcc gtcgttctgt acctcaatag ttacagcata aacaagtgct
     2401 actctttctt cttttgtctg gattattttg ggagtaaatt ctgctttttc cgaaatatgg
     2461 gttactttcc cgcttagagt tttataacct tttttcccgt catctattct tacagtacat
     2521 tcctgtccgg tttttatttt atccagctga gctccgctca catacacttt aagtttcatc
     2581 accgacaggt cggctatttt tgccagaggc ttgcctgctg cagccatttc accagtttcg
     2641 caatattttt gcaggatagt tcctttcaga gggctgcgca ccatgcatcg tgatatctgc
     2701 tcttcaaggg tggctttttt tgcattgtac actgccactt ctgcctgtac agaagccttc
     2761 tgggtattat ttgcggttat ctgtttcctg agtacctcca cctgtccggt gaggtcgtcg
     2821 tattgttttt ttgtggcagc atcatccttc aacatgtttt caattctttt aatatttacc
     2881 tgcaggttct ctatttgctg ttccagaatg gcattctgag cgttaatgga actgatgcgg
     2941 gttgttatgc ctgcaatgcc tgcgtcgatt tcagctttct gaaggtggaa aagtgtggtg
     3001 tcgataaggg ctatgagagc tcccttttct atttccatac cttcacgtat gtcgcattga
     3061 agtattcgtc cgttggtttc tgcagagact ataacttcag tcacttcgaa gtttccgtat
     3121 gcatcggctt cgcttttgtt gttactgcaa cctgctataa gcagtgctgc tggaagaatg
     3181 agagagatta ttctgttcat gattttccag tttattttat ttcctgtccg cttatgttaa
     3241 aatattctgc ttttgccatt gcaaggctta ctttatgcag ttcgtatgtt acagcagcct
     3301 gtcgttcggc gttgagttca cccagcatct cggcagcggt tataactccg ttttcatacc
     3361 gggatatagc tgctgacgaa attttctttc tcagttcaat aagttccctg tctgattcca
     3421 gcagggattc gagcgacaga atctctgcct tttttgcttc gagcatcctg cgcagattct
     3481 cttccaggtc ggtttttctt ttttcaataa tgttttgctc gagagtaagg atttcctttt
     3541 cgttcttcac ccggctccaa tcaaagatat tccattttat tccggcacct ataatataat
     3601 atggtgcaaa ttcatcttta aggaagttat ttccgggagg atttccgtag cccagtgttg
     3661 caaagccgta ggcttttggc attcttcgtg ttgcgatcgt ttttattcct gcctgcagct
     3721 gttcttttct gatgtcgaaa tactgcagtt ccggacgcat caattcgata tctgtttctc
     3781 cgggcagttc aggaacagca agctggtccg caggttcaac agctataccg gtgaggtctg
     3841 acaggatttt aagaagagat gtttttttga tttcgatttc ttttagttgc tgttctatct
     3901 tgattttctc agctttcata atatctatat cagagggcac gattatttcg ttttttagtg
     3961 ctgattccat tgaagcaata cgtttttcta tcacttccct gaaaccttca aacgactctt
     4021 tctgccggtc gagcatcagt atggagaagt agtaattatt aaccagtgag cgtagtttgt
     4081 aaagatctgt ttcagtttgt ttttcattta ctttgcggtt tgtgttctca agttctcttg
     4141 ctgctttggt ggctcctccg tcgtatattg tctgggtaat gtcgacggtt atccggtact
     4201 gttcgtgagg caggggtgtg atggcatcag caagtccggg tataggaaca gtggaaaatt
     4261 tttcaccaag gtctataact tcggagttat atatgaatga agcacctgca tagagtgatg
     4321 gcagccagtt ctttgaaagg tttttgtctt ttatctgcca tattgaagta tggagttcct
     4381 gttctccggc aagtgagctt ttcttcattg cggcgtcgta acaatcggac agggtgatga
     4441 ttttctgcgg gtatcccttc agaattaata caaatgagac tattactgtc aggcaaatcc
     4501 tctgtatcat ggtttttatt ttttggtttt ccttctgttt tttaaggcgc ttaaaacaaa
     4561 atcgggtgca aattcttttc tttcttcgag atattcgtcg aaatttttgt tgaaattctc
     4621 cataatgact tcgaaaaatt ttttccgata aaggggaata tgcttattgc tgcaattgat
     4681 gtcattatct gtagcaggga gtcgggggtg atatcatatt tttcgaattc ctttccgtgc
     4741 tgttcagaga gtgttttgta gaaaagttta aagtcgatgt ttcttattaa acgttgaatt
     4801 ctttcgggtt tacggttaag ttcgttaatt ata
//
LOCUS       DAOSNB010000199         4797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17787_length_4797_cov_4.036693, whole genome shotgun sequence.
ACCESSION   DAOSNB010000199 DAOSNB010000000
VERSION     DAOSNB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17787_length_4797_cov_4.036693"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1378
                     /locus_tag="PLG42_08240"
     CDS             341..1378
                     /locus_tag="PLG42_08240"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOU31234.1"
                     /translation="MSKKKRPQKIGILTAGGDCPGINAAIRGVGKTAIVKYGMEVIGL
                     CDGFLGLINKEYITLTESSLSGILTLGGTILGTSREKPFKNSKGKKDISKPALMVEHY
                     EQLGLDCLVCIGGNGTQKTAHLLSQEGLNVIGIPKTIDNDVWGTDITFGFDSAVNIAT
                     EAIDRLHSTANSHKRIMVIELMGHNAGWLALYSGVAGGGDVILIPEIQYNENIIKDYL
                     LKRSRENKPYSIVVVAEGIKIPSKEKSAGNYIARRINQITGLDTRVTVLGYIQRGGTP
                     SPMDRILASKFGATAANMIVDRDYGKMVSQKNGDITSVPLAEVAGKTRLVERNNTLLV
                     RAKEMGTCFGI"
     gene            1416..1901
                     /locus_tag="PLG42_08245"
     CDS             1416..1901
                     /locus_tag="PLG42_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOU31235.1"
                     /translation="MKRIIFMRHGKAENQETGLSDFERSLTTKGKNLSHQMTVKLKEK
                     ISNPGTLITSPAFRALETALIFAMEYDMKPEEIIIKSEIYYRFNEKSLLTVLDSAAKN
                     SDSVVLFGHDPSFTNIANYLSGNSVGEIPKSGIVCLSFNVKEWDEIKAGSGVPELILR
                     P"
     gene            1911..3947
                     /gene="ppk1"
                     /locus_tag="PLG42_08250"
     CDS             1911..3947
                     /gene="ppk1"
                     /locus_tag="PLG42_08250"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOU31236.1"
                     /translation="MAKDYIPKEISWLSFNERVLQEAENKEVPLLERLKFLGIYSNNL
                     DEYFRVRVATLKRLTYYNNAREILGYDPRKTLKKIQKIVLSQNQVFEKIYSDIIKELA
                     KNKIHIINEQQLDKAQSDFVAEFFHSEVRTRLMPFMIEPESNLPNLTDDAIYLAILLR
                     KKKSNKRKYALLEIPSDVLPRFIVLPDRDNEKYLIFLDDVIRFGLKEVFVVFDFDEIT
                     AFTIKLTKDAEIEIADDYIASYIDKLSRSLQQRKKGTPVRFIYDREMPADLLKTITKK
                     LKLGPDDVIIPGNRYHNFKDFMNFPNLGRKKLYYEPLVPIQHKDITPGKSILSAIQKK
                     DIMLFFPYHPFDHFIDLLREASIDPYVTSIQITLYRLARNSSVINALMNAVRNGKSVT
                     TVVELQARFDEEANILWGNKLMNEGVKVIYGVPGLKVHAKLCLITRVKDEVTRKYAAI
                     GTGNFNEDTARIYTDHLLLTCEKKITNEVQKVFNFFHANYIKENYYHLVLSPFHLRNK
                     MILLIENEIKNAKARKKAYIWLKLNNITDREIIDKLYEASQAGVEIRMIIRGMFSIVA
                     GVKGLSENIQAIGIVDRFLEHTRFMIFCNDGDEEIYISSADLMTRNLDHRIEVTCPVF
                     DENIKKHLKKVFEIQWKDNTKARIIDPALSNKFVTNDKKKINSQTEIYNILKEE"
     gene            3981..>4797
                     /locus_tag="PLG42_08255"
     CDS             3981..>4797
                     /locus_tag="PLG42_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOU31237.1"
                     /translation="MKFILRYILLQAIVMGLHLPAITQDVKDFEYYRRLNEQEKRLPE
                     YKDSDDDLRLKLQQLEIINNSRRKHRAQPLELDILASRVANKICREAAENEYVSHWNL
                     KGEKPYHRYAFAGGHDHVTENAYGEWDSKGFTVTPELIAKLMKNGHESFMAERAPNDG
                     HKRNIIDKAHNYAGIGFYITKNHFRYYEEFINRYLEYISVPTALSTGETGTIVIDTKG
                     RSYLYFLICYREPFPVPLRPGQKPRKGSYEDFSKEVAFKVPAWELAGYRKGNIY"
BASE COUNT         1650 a          971 c          986 g         1190 t
ORIGIN      
        1 gctgcaagga aatacaaagg ggggtctgtt tatactacaa ggaaatacaa atgtaggtac
       61 ctatcaatta ataaaataca ctggtagaat agtctctcca caacacaggg tgcattttca
      121 aacatgaatt ttatccctgc ttattttatt ttcaaattta attttaagtc atgtgaattt
      181 catagttgtt attgccgctc tcttttgtat atttgaattt aataagttat tgtctgagag
      241 taaaacggta atgtaataaa cctggttttt acaaaagaat tcagcaaatt ttgctaaaga
      301 aaataaactt aagcattaat taacagaata agaatctgaa atgagcaaga aaaagagacc
      361 tcagaaaata ggcatactca ctgcaggagg cgattgtccg ggaataaatg cagccatcag
      421 gggtgtgggc aaaacagcca ttgttaaata cgggatggaa gttattggac tgtgcgatgg
      481 cttcctcggg cttatcaaca aggaatatat tactcttacc gaaagcagcc tatccggtat
      541 cctcacactc ggcggaacta ttcttgggac ttcgagggag aagccattca aaaacagcaa
      601 gggtaaaaaa gatatcagca aaccagccct gatggttgaa cattatgagc agctgggtct
      661 cgactgcctt gtgtgtatag gtggtaacgg cacacagaag actgcacatc tgctctcgca
      721 ggaaggattg aatgtgatag gcatacccaa gaccatcgac aacgacgtat ggggtaccga
      781 catcactttc ggtttcgact cagcagttaa tatagctacc gaagctattg acaggcttca
      841 ctccacagcc aattcgcata aaaggataat ggtgatagaa cttatggggc acaatgcagg
      901 atggcttgca ctctattcag gagtggctgg gggtggcgat gtcatcctca tccctgaaat
      961 acaatataac gaaaacatta ttaaagatta tttgctgaaa aggtctcgcg aaaacaaacc
     1021 ctattcaatt gttgttgtag ctgaaggaat aaaaattcca tcaaaggaaa aatcggccgg
     1081 gaactatata gccagaagaa tcaaccagat tacaggcctc gacacgaggg ttaccgtact
     1141 cggatatata cagaggggcg gcacaccttc gcctatggac agaatactgg cgtcaaagtt
     1201 tggggctacc gcagccaata tgattgtcga ccgagactat ggcaaaatgg tgtcacagaa
     1261 aaacggcgac ataacttcag tgcccctcgc agaagtggca ggaaaaacaa ggctggtaga
     1321 acgaaataat actctacttg tcagagcaaa agaaatggga acatgttttg gtatttaaaa
     1381 actgtataaa actagctaaa actaacctca taaaaatgaa aagaataatt ttcatgcggc
     1441 acggaaaggc agagaatcag gaaactggac tctccgactt tgaaaggtct ctcacaacaa
     1501 aagggaagaa tctttcacac cagatgactg ttaaacttaa agagaaaata agcaatccgg
     1561 gcacccttat caccagtccg gctttcagag ccctcgaaac agccctcatc ttcgccatgg
     1621 aatacgacat gaaaccagaa gagataatta ttaagagtga aatttattac aggtttaacg
     1681 aaaagtcact gctgacggta cttgattctg ccgcaaaaaa cagcgactca gtggttcttt
     1741 tcggtcacga cccctctttt accaacattg ccaactacct gagtggaaat agtgtcgggg
     1801 aaatccccaa aagtggaatt gtctgtctct ccttcaatgt taaggagtgg gatgaaataa
     1861 aagccggctc aggtgttccc gaattaatac tacggcctta acaaccagtc atggcaaaag
     1921 actacatacc aaaggaaata agctggctct ctttcaacga gagagtactt caggaggctg
     1981 aaaacaaaga agttccttta ctcgaacgtc ttaagtttct cggcatctat tccaataacc
     2041 tcgatgaata tttcagggta agagtcgcta ccctgaaaag acttacttat tacaataacg
     2101 ccagggaaat tctcggttac gaccccagaa agactcttaa aaaaatccag aagatagttc
     2161 tgtcgcagaa tcaggttttc gagaaaatat attccgacat tataaaagaa ctcgcaaaga
     2221 acaaaattca tataattaac gaacagcaac tcgataaagc ccagtcggat tttgttgctg
     2281 aattcttcca cagtgaggtc agaacccggc tgatgccctt tatgattgaa cccgaatcaa
     2341 atctgcccaa cctcaccgat gatgcaatct atcttgcaat tctgctgcgg aagaaaaaaa
     2401 gcaataaaag gaaatacgcg ctactcgaga taccatcaga tgtacttccc cgcttcattg
     2461 ttctacctga cagagacaat gaaaaatacc tgatattttt agatgatgta atcaggtttg
     2521 gacttaaaga agtattcgtt gttttcgatt ttgacgagat aactgctttt accataaaac
     2581 tgaccaagga tgcagaaata gaaattgctg atgattacat agccagctac atcgataaac
     2641 tgtcgcgcag ccttcagcaa agaaaaaaag gtactccggt aaggtttatc tacgaccggg
     2701 agatgccggc agacttacta aaaaccatta caaagaagct gaaactcgga cccgacgatg
     2761 ttatcatccc gggtaaccgc taccacaact ttaaagactt catgaatttc cccaaccttg
     2821 gtcgtaaaaa gttgtattat gaacctcttg ttcccataca gcacaaagat ataactccgg
     2881 gtaaaagcat cctgtcagct atacagaaaa aagacatcat gctctttttc ccgtaccatc
     2941 ctttcgacca cttcattgac cttctcaggg aagcatccat cgacccttat gtaacatcaa
     3001 tacagataac tctataccgc ctggcccgca actcaagtgt aataaatgcc cttatgaatg
     3061 ccgtaagaaa cggtaaaagt gttaccactg tcgttgaact ccaggcacgg ttcgacgagg
     3121 aagctaacat tctctgggga aataagctga tgaacgaagg agtcaaggta atctacggcg
     3181 tacccggact caaagtgcat gcgaaacttt gccttatcac aagagtaaaa gacgaagtca
     3241 caagaaaata tgcagcgata ggcacaggaa atttcaacga agacactgca cgtatctata
     3301 ccgatcacct gctactgaca tgcgaaaaaa agataacaaa tgaagttcag aaagtattta
     3361 atttctttca tgccaattat ataaaagaaa attactacca cctggtttta tcaccttttc
     3421 atctgcgcaa caaaatgatt cttcttatcg agaacgaaat aaaaaatgca aaagccagga
     3481 aaaaagctta catatggctg aaactaaata atataaccga cagggaaata attgacaagc
     3541 tttacgaggc aagccaggct ggagtagaaa tacgcatgat tataaggggc atgttctcaa
     3601 ttgtagcagg ggtaaaagga ctgagtgaaa acatccaggc aataggtatt gtcgacagat
     3661 ttctggaaca tacaagattc atgattttct gcaacgatgg tgatgaagag atttacatat
     3721 catcggcaga tcttatgacc aggaatctcg atcaccgtat tgaagttaca tgtccggtgt
     3781 tcgacgaaaa cataaaaaaa catcttaaaa aagtctttga gattcagtgg aaagacaata
     3841 ccaaagcaag gataattgat cctgcgctga gcaacaaatt tgtcaccaac gacaaaaaga
     3901 aaataaactc gcaaacggaa atctacaaca tactgaagga ggaatgattt tttaaataat
     3961 tattccggga aatttttctg atgaaattta ttttacgtta catactgctt caggcgattg
     4021 ttatgggact gcatctgcct gctataacac aggatgtaaa ggattttgaa tattacagaa
     4081 gactgaacga acaggaaaaa aggcttcctg aatataagga cagtgatgat gacctcaggc
     4141 ttaaacttca gcaactcgag ataattaata acagcagaag aaaacacagg gcgcagcctc
     4201 ttgagctcga cattcttgcc tcacgagttg caaataagat ttgccgtgaa gcagctgaaa
     4261 atgaatatgt ctcacactgg aatcttaaag gtgaaaagcc ttatcaccgt tatgcttttg
     4321 ctggaggtca cgaccacgtt acggaaaatg cctatggtga atgggattca aaaggattta
     4381 ctgtaacacc tgaactgatt gcaaaactca tgaaaaacgg ccatgaaagt tttatggctg
     4441 aaagagcccc caacgacgga cataagcgca acataatcga taaagctcat aactatgcag
     4501 ggattgggtt ttacatcaca aagaaccatt ttagatatta cgaggaattt ataaacagat
     4561 accttgaata cataagtgtt ccgacagcat tatcaacagg agaaacagga actatagtaa
     4621 ttgacacaaa agggaggtcg tacctttatt ttctgatatg ttaccgtgag ccctttccgg
     4681 tgcctctccg tcccggccag aaacccagaa agggaagtta tgaagatttt tcgaaggaag
     4741 ttgcgtttaa ggtccctgca tgggaacttg ccggctacag gaagggaaat atttatt
//
LOCUS       DAOSNB010000200         4781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_17857_length_4781_cov_4.302793, whole genome shotgun sequence.
ACCESSION   DAOSNB010000200 DAOSNB010000000
VERSION     DAOSNB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17857_length_4781_cov_4.302793"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLG42_08260"
     CDS             complement(<1..182)
                     /locus_tag="PLG42_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOU31238.1"
                     /translation="MKNHRNLSRKSGGLTRRTFLGRSAAAAAAFTIVPRHVLGGKGYT
                     APSDMVNVAGIGVGSQG"
     gene            complement(747..4229)
                     /locus_tag="PLG42_08265"
     CDS             complement(747..4229)
                     /locus_tag="PLG42_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOU31239.1"
                     /translation="MISLAFAISVATNSQVISKTEPSKKVALVIGNGNYMLGTLANPE
                     NDAKLIADVLKKLEFDVMFYTNLDWRSMKKSLDDFRAKLKGADVALFYYAGHGVQVGG
                     YNFLIPVDANPQSESQVEYDCIPADRILSDMTDSGAKIKIMILDACRNNPFEKRWSNP
                     SSSRGLAVMRASSRSNNTFIAYSTAPGSTALDGSGKNSPYTTALAEAMVIPDIKINDM
                     FTIVAKNVSQRTGGEQNPWKSDSMVEDFYFNPQSMISPAEGLDYFYDARDKNNYQILK
                     TNSQVWTKRNLNYVSFNNGDSIPVISDPEQLRLLNMPVILWSPQFRNAGKLYNYYTVT
                     DVRNICPAGWHIPTSEEWKILANETGLKLKSLDGDKLNMETEVMNQPQVRNVIKPSGV
                     SALPSRLREEIMEFVGQGSDRGFFASDGYVITISADSVKIDSLLDMSDARSIRCVKGR
                     SPIAATDSSSDILATSAVVYGRVNPNLAYTFVSFEYGLTKDYGQSVDAEEISVTGSVP
                     SVVTAKISGLTPGSVYHYRVRAENNAGTSYGEDRTFTTLVPPFASTQPATSISSSEAT
                     LNALVNANNAVTEVSFEYGLTPEYGNIVTATGNPFTGNEKVLVNCTIKGLSENTVYHY
                     RVKAVSKAGTTFGNDMTLTTLKLPVVKAEVPAAVKSYSAVLRGTINSSNLPTDISFEY
                     GTGEDYDRSIKGSETPMQSNKDEPISISLDSLMPGTEYHYRIKAYNIAGTSFSNDMTF
                     TTPEVVTDVEGNSYVTLIIGDQIWMAENLRTTKFADGTVIPEVKGKNAWKNRMKAAYC
                     WYDNDTSYRKVTGALYNWYTVLATNMLCPKGWHVPTRSEWDILLNSMGGYEKAGSKLK
                     ERGTAHWQDPNRDATDESGFTALPGGVRNIGGLFSYYGIKGQWWTSDDTVKNLAWYQA
                     MDFNNTAVNRFFTVKSEGMSVRCVKYKIPEVKSQPPDSVINPPPVVEEPVYFVDPRDD
                     RQYQIVKIGKQVWMAENLVATKFNDGTEIPEVVDKIEWTRLVTPAYCWYDNHEAYSKY
                     GALYNWQAVSSGKLCPVGWHVPTYEEWEELFNTLGGKAIAGGTLKESGTAHWDKPNMS
                     SGYISRFAALPGGYRNEYGDFSDIGKKGFWWSYTRNKKPAGWYQVMYNSNTSVVTRNF
                     QDKPVGFSVRCVKD"
BASE COUNT         1374 a         1130 c          830 g         1447 t
ORIGIN      
        1 ccctgactgc cgactcctat gcctgccacg ttaaccatgt cgctaggggc tgtataaccc
       61 tttcctccaa gcacatggcg cggtacaatg gtgaaagcag ctgctgctgc agccgacctg
      121 cccagaaagg ttcgcctcgt caaacctccg gactttcgtg aaagattacg gtgattcttc
      181 ataaacagtt agttttaaga ttaccagtcg aactcctgat taatatccat aaaattatgc
      241 aaaagaaata tcgcacataa aaaattaagg aaaattaacg tttcggaaac aatttgagag
      301 tcttgcaatg gatatgttct gggtttcaac taatatcagg aaaaaataga ggttgttctt
      361 gaaaattatt taaactgtta tattgaagag ttgtgaagag gaactgaatg aaaaggaaat
      421 gttattttca taaattaaat attaatgttt ttccttaatt cgtatatttt aataaccggt
      481 tattaaagtc aaagttaaag aattctgaca aggaaaagat aactaatcgg gtttgtttaa
      541 gaatcacatt ctactcctgc gaagttcaca acaaatggcc tccaccgtaa aacatttcct
      601 gatatgaaaa aaataatatt ctcctgtact tataaaatga aatctttccc atccacttac
      661 tgccataaaa cctgcaaacc cttcaacctg taaaccagct aaccagtaaa cccgttaacc
      721 cgtaaaccag ttaaccctca aattttctaa tccttaacac accttacaga aaaccctacc
      781 ggcttgtctt gaaaattcct tgttacaact gaagtatttg aattatacat tacctggtac
      841 catcctgcag gttttttgtt tcttgtataa ctccaccaaa atcccttctt tccgatatca
      901 gaaaaatctc cgtattcgtt acggtagcca ccaggcaggg ctgcaaaccg gcttatataa
      961 ccagaagaca tgtttggttt gtcccagtga gctgtgccag actctttcag agtacctcct
     1021 gcaatcgcct tcccgcccag agtattaaag agctcctccc actcctcata cgtcggaacg
     1081 tgccatccta ccggacaaag cttacccgaa ctcacagcct gccagttgta aagtgctcca
     1141 tatttgctgt aagcctcgtg attgtcgtac cagcagtagg ccggagtcac aagccttgtc
     1201 cattcaatct tatcaacaac ttcaggaatt tcagttccat cattgaactt tgttgctaca
     1261 agattctctg ccatccatac ctgttttcct atttttacta tctgatattg cctgtcatcc
     1321 ctcggatcaa caaagtaaac aggttcctca accacaggag gaggattaat gacagaatca
     1381 ggaggctggc tcttaacctc cggtatttta tacttaacac atcttaccga cattccctct
     1441 gatttgactg taaaaaatct gtttactgcc gtgttattga agtccattgc ctgataccag
     1501 gctagattct ttacagtatc atcacttgtc caccactgac cttttattcc gtaatacgaa
     1561 aacaatcctc cgatatttct aacaccaccc ggaagtgcgg tgaaaccgct ctcatctgtt
     1621 gcatccctgt tgggatcctg ccagtgagct gtgcctcttt ccttgagctt tgaccctgct
     1681 ttttcatatc ctcccatgct attcagcagg atatcccatt cacttcgagt aggcacatgc
     1741 cagcctttcg ggcataacat gttagttgcc aatactgtat accagttata gagggctcct
     1801 gtaacttttc tgtacgacgt atcattatca taccagcaat atgctgcctt catcctgttt
     1861 ttccatgcat tcttaccctt cacttcagga attacagtgc catctgcaaa ttttgtcgtg
     1921 cgcaggtttt ctgccatcca tatttgatct ccaatgataa gcgtgacgta gctattcccc
     1981 tcaacatcag tgacaacctc tggtgtggtg aaggtcatat cattactgaa tgatgtaccg
     2041 gcaatattat atgcttttat cctgtaatga tactcagtac ccggcataag gctgtcgaga
     2101 ctgatgctta ttggctcatc tttattgctt tgcatgggag tttccgaccc tttaattgac
     2161 ctgtcataat cttcgccggt tccatactcg aaagaaatat ctgtaggcag gttgcttgaa
     2221 ttaattgtcc cgcgcagtac tgcagaataa gattttacag ccgcaggaac ttctgccttt
     2281 accacaggca gttttagagt tgtgagtgtc atgtcatttc caaaagttgt acctgccttg
     2341 ctcacagctt ttactctgta atgatataca gtattttccg aaagtccttt tattgtacaa
     2401 tttaccagca ccttttcatt tcctgtaaaa ggattgccgg ttgcagtaac aatattgcca
     2461 tattcaggtg tgaggccata ttcaaagcta acttctgtaa cagcgttatt tgcatttacc
     2521 agtgcattaa gtgttgcctc cgaagagctt attgatgtgg caggttgagt ggaggcaaag
     2581 ggtggaacaa gcgtggtaaa tgttctgtct tccccgtatg atgtgccggc attgttttct
     2641 gccctgaccc tgtaatgata tacgcttcca ggtgtaagtc ccgaaatttt tgcagtaacc
     2701 actgagggca cagaacctgt aacactaatc tcctcagcat caactgattg accataatct
     2761 ttcgttaatc cgtactcaaa agaaacaaaa gtatatgcaa ggtttggatt tactctgcca
     2821 tacactacag cgctagtagc cagaatatct gaagaagaat cagttgctgc aatcggactc
     2881 ctccccttta cgcatctaat ggaacgggca tccgacatgt caagcagact gtcgatttta
     2941 actgaatcgg cactgatggt tattacatag ccatcagaag caaaaaaacc tctgtcggac
     3001 ccctgaccaa caaattccat aatctcttct ctcagacgtg aaggtaatgc tgacactcct
     3061 gatggcttga taacatttct cacctgaggc tgattcatga cctcagtttc catattcagt
     3121 ttatccccgt ctaaagattt caatttcaga ccggtttcat tcgccaatat tttccattcc
     3181 tctgatgtcg gaatatgcca gccggcaggg catatattcc tcacatctgt tacggtgtaa
     3241 taattatata attttcccgc attccggaat tgaggagacc acagaattac aggcatgtta
     3301 agaagcctca attgctcagg gtcagatatt acagggattg agtctccgtt attaaaactt
     3361 acatagttca gatttctttt tgtccatacc tggctgtttg tttttaaaat ctgataattg
     3421 tttttatcgc gggcatcgta aaaataatct aacccttctg ccggagaaat catgctctgt
     3481 ggattgaaat aaaaatcttc aaccattgaa tctgatttcc atggattttg ttctcctcct
     3541 gttctctgtg acacattttt tgccacaatt gtaaacatgt catttatttt gatatcaggg
     3601 ataaccatag cttcggcaag tgctgtggta taggggctgt tttttccgct cccatcaagg
     3661 gcagtgctgc cgggcgcagt ggaataagca atgaatgtat tgttcgacct tgacgatgcc
     3721 cgcatcacag ccagacctct tgaagatgat gggttgctcc atctcttttc aaaaggatta
     3781 tttctgcagg catcaagaat cattattttg atctttgcac ctgaatcagt catatcactt
     3841 aatatcctgt cggcaggaat acaatcatat tctacctgtg actccgactg ggggttggca
     3901 tcaacaggta ttaaaaaatt atatcccccc acctgaacac catgacctgc gtaatagaac
     3961 agtgcaacgt ctgcaccttt aagtttcgcc ctgaagtcat cgagtgattt cttcatcgat
     4021 ctccagtcga ggtttgtata aaacattaca tcaaactcga gcttcttaag aacatcggca
     4081 ataagctttg catcattttc cggatttgca agtgtgccaa gcatgtagtt gccattccct
     4141 attaccagtg caaccttttt cgaaggttct gtcttactga ttacctgaga attagttgca
     4201 acagaaattg caaatgccaa tgaaatcaat aagatgattt taaaattcat aagttgccgc
     4261 agtaagtaat caaacttact aaatatttta ttatcagaca aaaaattaaa acaaaattta
     4321 ggattctgtt ctgctaaata ataataaata aaaaaggcac tgaaacacga acgaaaaaca
     4381 aataaccagt taacccgtca accagctaac catccaacca gctaaccagt taacccgtca
     4441 accagctaac catctaaccc gctaaccagt taaccagcta accagttaac cagttaaccc
     4501 gctaaccagc taaccagcta accagttaac ccgctaacca tctaacccgt taaccagtta
     4561 accagttaac catctaacca gttaaccagt taacccgcta accccaaaaa ccccgtaacc
     4621 ttataaaact ttctccgtat gttttctatt tttatcacat aaaaatttgc ctgaccatga
     4681 caataaaaat tctttctttt tcatttttgt ttgccatcac ctctctttgc acagcacaga
     4741 gcatgaaaga tgagtacaaa attcccataa gcgaaatttt c
//
LOCUS       DAOSNB010000201         4749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18006_length_4749_cov_3.989561, whole genome shotgun sequence.
ACCESSION   DAOSNB010000201 DAOSNB010000000
VERSION     DAOSNB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18006_length_4749_cov_3.989561"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1059)
                     /locus_tag="PLG42_08270"
     CDS             complement(67..1059)
                     /locus_tag="PLG42_08270"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOU31240.1"
                     /translation="MAILAFQKPDKVIMLEANDKFGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGYAITLVKIEGVDHEFSTIKGVIEDVTEILLNLKQIRFKRKTEDIESEKVVI
                     KVTGRDTFKAGDLNSVLAHFEVLNPDLVICRMEPSVKLNVELSINKGRGYVPAEENEP
                     ANPEFGVIAIDSIFTPIRNVKYTVENYRVEQKTDYEKLIIEIETDGSIHPKDALKEAA
                     KILIYHFMLFSDDKITLDSGEKMASEEFDEEILKMRQLLKTKLVDLDLSVRALNCLKA
                     AEVETLGDLVKFNKNDLLKFRNFGKKSLTELDELLESLNLSFGMDVSKYKLDKD"
     gene            complement(1067..1675)
                     /gene="rpsD"
                     /locus_tag="PLG42_08275"
     CDS             complement(1067..1675)
                     /gene="rpsD"
                     /locus_tag="PLG42_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOU31241.1"
                     /translation="MARYIGPRTKVARKFGEPIFGPDKYLERKNYPPGQHGQNKKRRK
                     VSEYGLQLREKVKVKYLYGLLERQFKNAFERASRSKGVTGEVLLQILESRLDNTVYRL
                     GIAPSRAAARQLVSHRHITVNGKVVNIPSYQLKPGDIVGVREKSKSLEVIVNSLSDRR
                     YQKVPWLEWDENLMAGKFMSVPQRADIPENINEQLIVELYSK"
     gene            complement(1728..2117)
                     /gene="rpsK"
                     /locus_tag="PLG42_08280"
     CDS             complement(1728..2117)
                     /gene="rpsK"
                     /locus_tag="PLG42_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOU31242.1"
                     /translation="MAKKTGALKRKNVRIEPAGQAHIHSSFNNIIVTITNNSGQVISW
                     SSAGKMGFRGSKKNTPYAAQMAAEDCSKEAYDLGVRRVKVFVKGPGAGRESAIRAIAN
                     AGIEITEIVDVTPLPHNGCRPPGRRRV"
     gene            complement(2127..2504)
                     /gene="rpsM"
                     /locus_tag="PLG42_08285"
     CDS             complement(2127..2504)
                     /gene="rpsM"
                     /locus_tag="PLG42_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOU31243.1"
                     /translation="MARIVGVDLPRNKRGEIALTYIYGVGRSTAQKVLDQAGVDRNIK
                     VKDWTDEQVNAIRKILSENYKLEGELRSEMQMNIKRLIDINCYRGIRHRLGLPVRGQR
                     TKNNARTRKGRKKTVANKKKATK"
     gene            complement(2539..2655)
                     /gene="ykgO"
                     /locus_tag="PLG42_08290"
     CDS             complement(2539..2655)
                     /gene="ykgO"
                     /locus_tag="PLG42_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOU31244.1"
                     /translation="MKVRTSVKKRSADCKIVRRKGRLYVINKKNPRFKQRQG"
     gene            complement(2661..2879)
                     /gene="infA"
                     /locus_tag="PLG42_08295"
     CDS             complement(2661..2879)
                     /gene="infA"
                     /locus_tag="PLG42_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOU31245.1"
                     /translation="MAKQPPIEQDGTIVEALSNAMFRVELENGHVITAHISGKMRMHY
                     IKILPGDKVRVEISPYDLTKGRITFRYK"
     gene            complement(2884..3660)
                     /gene="map"
                     /locus_tag="PLG42_08300"
     CDS             complement(2884..3660)
                     /gene="map"
                     /locus_tag="PLG42_08300"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOU31246.1"
                     /translation="MLICRTKEEIELLRESNLLVSRTLAEVAKHIAPGVTTKSLDRIA
                     ETFILDHGAVPAFKGYNGFPSTLCTSVNEEVVHGIPSDYILKEGDIISIDCGVILNGY
                     YGDSAFTFAVGEVSSEIRKLLEYTRASLEDGAKEAVAGNRIGDISFAVQTRAESGGFS
                     VVRELVGHGLGTKLHEPPEVPNFGKRGTGPKLLKGVVICIEPMINMGVKETLQKSDGW
                     TIVTADGKPSAHFEYAVAVDTGKADVLTTFKYIDEVLNKK"
     gene            complement(3679..>4749)
                     /gene="secY"
                     /locus_tag="PLG42_08305"
     CDS             complement(3679..>4749)
                     /gene="secY"
                     /locus_tag="PLG42_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU31247.1"
                     /translation="IVVQLLGIIFPYFQKLQKEGESGRRKLNQYTRYLTVLILVLQAP
                     TYLYNLQARLPASAFIISGTFFLVSSVIILTAGTIFVMWLGEKITDKGIGNGISLLIM
                     IGIVARLPANFIFEAGARMNGAGGAIALVVEMVFLFVVILGTILLVQGTRRVPVQYAR
                     RIIGNKQYGGVRQYIPLKVNAAGVMPIIFAQAIMMLPIIIAGYSNSSSGFVVAFSNMY
                     GFWYNLVTGLLIILFTYFYTAITINPVQMAEDMKKNGGFIPGIKPGRKTVEFIDQIIS
                     RITLPGSIFLAIIAILPAIAVKAGVSPQFAQFYGGTSLLILVGVILDTLQQIESHLLM
                     RHYDGLMKSGRVKGRTGAVAAY"
BASE COUNT         1181 a         1102 c          923 g         1543 t
ORIGIN      
        1 atagccttcc tgtgagaact tgtccttccc aaatggttaa ttactctctg atgtcgcatg
       61 gcttaattaa tctttatcaa gtttatattt actcacatcc ataccgaacg agagattcag
      121 tgattcgaga agttcatcca gttcggtaag agattttttc ccgaagtttc tgaatttcaa
      181 caggtcattt ttgttgaatt tcacaaggtc gcccagtgtt tcaacttcgg ctgctttcag
      241 acagttgaga gctctgaccg aaaggtcgag gtcaacaagt tttgtcttga gcagctggcg
      301 catcttaagg atctcctcat caaactcctc acttgccatc ttctcacccg aatcgagggt
      361 aatcttatca tccgagaaaa gcataaaatg gtaaatcaga atcttggctg cttctttcaa
      421 agcatctttc ggatgaatgg agccatctgt ctctatttca atgataagtt tctcataatc
      481 agttttctgt tcaacacggt agttttcaac cgtgtatttt acatttctga ttggcgtgaa
      541 tattgagtct attgcaatta ccccgaactc agggttggca ggttcattct cttcagcagg
      601 cacataccct cttcccttgt taattgacag ttcaacatta agctttaccg agggttccat
      661 tctgcatatt acaaggtcgg gattgagtac ttcgaaatgt gcaagcactg aattaaggtc
      721 gcctgctttg aaggtatccc ttcctgtaac ttttattaca accttttccg actcgatatc
      781 ttctgttttt cttttgaatc tgatctgttt aaggttaagt agtatttctg ttacatcttc
      841 aatgacgcct tttattgtcg agaattcatg gtcgactcct tcgattttca ccagtgttat
      901 agcataaccc tcgagggaag aaagaaggat tctccgcagg gcatttccga ttgtaatgcc
      961 atatcccggc tcgagcggac gaaattcgaa tttgccgaat ttgtcgttcg cttcaagcat
     1021 tatgacttta tcaggtttct ggaatgctaa gatggccatg gttattttat ttggaatata
     1081 gttcaacaat taattgttca ttaatatttt ctggaatatc ggcgcgctgc ggaaccgaca
     1141 taaattttcc ggccataagg ttttcatccc attccagcca gggtactttc tggtatctgc
     1201 ggtcagatag ggagtttact ataacctcaa gtgattttga cttttcgcgg acgccaacaa
     1261 tatcaccagg tttaagctgg tatgatggaa tatttacgac ttttccgtta acagtgatat
     1321 ggcggtgcga aacaagctgg cgtgctgcag cccttgaagg tgcaataccc agacggtaaa
     1381 cagtgttgtc aagacgtgat tccagaatct gcaacagtac ttcaccggtt acgcctttgc
     1441 ttctggaagc acgctcaaat gcattcttga actgtctttc aagcaatccg taaagatatt
     1501 tgacctttac tttttccctt aactgaagac cgtattccga tacctttctt cttttcttgt
     1561 tctgaccatg ctggcctgga ggataatttt tcctttccag gtatttgtca ggtccaaaaa
     1621 taggttctcc gaattttctc gcaacttttg ttcttggtcc tatatatcta gccatttatt
     1681 ttattatatt aaaattcaac ttaaacaatt gttaatcttt tagttaatta aactcttctt
     1741 cttcctggag ggcggcatcc gttatgtgga agtggtgtaa catcaacgat ttccgttatt
     1801 tcgatgccgg cattagctat tgcccttata gccgattcac gtccggcacc gggtcctttc
     1861 acaaatactt ttactcttct cactccaagg tcataagcct ccttgctgca gtcctcagca
     1921 gccatctgtg ctgcataagg agtatttttc tttgaacctc tgaaacccat tttcccggct
     1981 gatgaccatg aaattacctg acccgagtta ttggttatgg ttacaataat attgttaaat
     2041 gatgaatgaa tgtgagcctg tccggctggt tctatccgta catttttcct ctttaatgct
     2101 cctgtcttct tcgccatatt caaatcttat ttagtagcct ttttcttgtt tgcaacagtt
     2161 tttttccttc ccttacgggt acgggcattg tttttggtcc tctgtccacg taccggaagg
     2221 ccaaggcgat gacgtatgcc tctgtaacag ttaatgtcaa tcagcctctt gatgttcatc
     2281 tgcatctccg aacgaagctc accttcaagc ttgtaattct cgctcagtat ttttctgata
     2341 gcattaacct gttcatcagt ccaatcctta accttgatgt tacggtcaac acctgcctga
     2401 tcaagcactt tctgagcggt gctccgtcca acaccataga tatatgtgag ggctatttca
     2461 cccctcttgt ttcttggtaa atctacccca actatacgtg ccataactct tttttaatag
     2521 tttgcaaagt tagtcatttt atccctgtct ttgtttaaat ctgggatttt tcttattaat
     2581 aacgtacaaa cgtcctttcc gcctgactat cttgcagtcg gcgctgcgtt tttttactga
     2641 tgttcttact ttcatatatc ctatttatat ctgaatgtta ttcttccttt agtcaggtcg
     2701 tagggtgata tttcaaccct gaccttatcg cccggtaaaa ttttaatgta atgcattctc
     2761 attttaccgg atatatgtgc tgttatcacg tgtccatttt cgagttcaac cctgaacatg
     2821 gcatttgaca gggcctcaac aatggttccg tcctgttcta tcgggggttg cttggccata
     2881 ggtttatttt ttgtttaaca cttcgtcgat gtatttaaat gttgttagca catctgcttt
     2941 tccggtgtct actgccacgg catactcaaa atgtgccgaa ggtttaccgt ctgctgtaac
     3001 gatagtccat ccatcgctct tctgcagtgt ttctttaact cccatgttta tcatgggttc
     3061 gatgcatatt accacacctt tcagcagctt tggccctgtt ccccttttgc cgaaattggg
     3121 cacttcgggg ggttcatgaa gcttcgttcc caggccatga ccaaccagtt ccctcaccac
     3181 tgagaatcct ccgctctctg ctctggtctg tacagcaaac gaaatatctc ctattcgatt
     3241 tcctgccacg gcttcctttg ctccatcctc aagagaagcc cgggtatatt cgagaagttt
     3301 tctgatttcg ctgcttacct cacccacagc aaaagtaaaa gcactgtcgc cataataacc
     3361 gttcagaatt actccacagt cgatcgagat gatatctcct tctttcagga tgtaatctga
     3421 cggtatgcca tgtaccacct cttcgttaac tgaagtgcac agtgttgacg ggaatccgtt
     3481 atagcctttg aaagcaggga cagctccatg atcaaggata aatgtctctg ctattctgtc
     3541 gaggcttttt gttgtgactc ccggggcgat gtgctttgca acttcagcaa gtgtacgcga
     3601 caccagcagg ttgctttccc tcagcagttc gatctcttct tttgttctgc aaatcaacat
     3661 caaagaacgt taagcagatt aatatgctgc aacagctcct gttctgcctt tcaccctgcc
     3721 cgacttcatc agaccgtcat aatgcctcat aagcaaatga ctctcaattt gctgcagggt
     3781 gtcaagtatc acaccaacaa gaataagtag tgatgtgcca ccgtaaaact gcgcaaactg
     3841 aggagataca cctgctttaa cagctattgc cggcagtatg gctataatag caagaaaaat
     3901 ggaaccgggt agtgtgattc tcgatataat ctggtcaata aactcaaccg tctttctgcc
     3961 cggtttgatg ccgggtataa accctccgtt tttcttcata tcttcagcca tctgaaccgg
     4021 attgatagtg atagctgtgt aaaagtatgt gaaaaggatt ataagcaatc ctgtcacaag
     4081 gttgtaccag aatccataca tatttgaaaa tgccacaaca aagcccgagc ttgaatttga
     4141 atatcctgca ataataatcg gaagcatcat aattgcctgg gcaaagatta ttggcataac
     4201 gcctgcggca tttactttaa gaggtatata ttgtcttaca ccgccatact gtttgttgcc
     4261 aattatgcgg cgtgcatact gaacgggcac ccggcgggta ccctgcacaa gcagtattgt
     4321 gccgaggatc acgacaaaga ggaaaaccat ttccacaaca agggcaattg caccacctgc
     4381 accattcatt cttgcacctg cttcaaatat gaagttcgca ggcagtcgtg caacgatgcc
     4441 tatcattata agaagcgaaa tgccgttacc gatacccttg tcagtaattt tttccccgag
     4501 ccacattacg aatatagttc ccgctgtaag aattatcacc gatgaaacca gaaagaaggt
     4561 tcctgatatg ataaatgctg atgcgggaag ccttgcctga aggttataaa ggtatgtggg
     4621 tgcctgaagt acaagtataa gaactgtaag atatcttgtg tactgattca gttttcttct
     4681 gccgctttcg ccctctttct ggagtttctg gaaataagga aaaattattc cgagaagctg
     4741 cacaacgat
//
LOCUS       DAOSNB010000202         4729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18106_length_4729_cov_3.773000, whole genome shotgun sequence.
ACCESSION   DAOSNB010000202 DAOSNB010000000
VERSION     DAOSNB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18106_length_4729_cov_3.773000"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2805
                     /locus_tag="PLG42_08310"
     CDS             <1..2805
                     /locus_tag="PLG42_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU31248.1"
                     /translation="TDIYRARQTVTERLYQVSEQLPADAGKPVLAPQSSLLGEMMIIA
                     VTSDSLNPMDLRTFAGWSITPRLLSIPGVAQVHVIGGDEKEYQILADPLKMNYYNVSI
                     DELLEACRNINENSPGGFINQHGNKYIVRGIGRTSDPGDIAAGVIKTVNNQPVKIGNV
                     AEVTIGSSPKIGTASYNGHNAVLITVTRQPHANSVKLAGEIIRVTDEIQKNAGNKIVI
                     HTGVYNQAAFIKTAINNVLKALTEGGIFVILILFIFLFNFRTTFISVLAIPFSLLVSV
                     LTLRLLGYTINTMSLGGMAIAIGSLVDDAIIDVENSWKQLRLNALKHGTEKEHPLRVI
                     LRASVEMRSSIVNATAVIIVTFLPLFLLEGFEGRMLKPLGIAFIVSLFASMVIAITVT
                     PVLCSYLLTNEKRLHKLARGAWVERNLTSAYKKVLEPALKRPGIIIASAATLLAVSVI
                     MLVTSGSSFLPPFNEGALTVNIGLMPGTSIEESDKIGRMAEEILLGIPEVKAVSRKTG
                     RAELAEHSFGENFSELDVPFELRKRSRADFFADVRSRLAKLPGASVEVGQPITHRIDN
                     MLSGSKANIAIKIFGDDLNVLYNLAQEIKHEISDIKGIADLTVEQQVETPQLKIKPRR
                     ELLAMYGIPAGKFTSFIRCMLGGETISEVYEEEKRFPMVVRYNDETRASIEGIKSSLI
                     DTHDGRKIPLTMVAEIESSSGPYSINRENVQRRIVISVNVSGSDQGTVAKNIRNRIEK
                     NITLPVSYRIEYSGQFENAARAARRLLLASVAALIAVFMILYNEFRKKSLALIILLNL
                     PLALIGGFFAVKLSSGVVSIPAIIGFITLTGIATRNGILLVSRYTHLKEEGVPIKERI
                     IRGSADRLNPILMTALTAAFALIPLALGGDKAGNEIQSPMAVVILGGLLSSTLLNLFV
                     VPAVYSVAEKNSSQENAKSE"
     gene            2873..3997
                     /locus_tag="PLG42_08315"
     CDS             2873..3997
                     /locus_tag="PLG42_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU31249.1"
                     /translation="MNSFLREVAENNPEIIAYRKMLEARKAEARTNLYPSGPEASFGY
                     MPGSGSTAGTKKTWSVSQKFDFPAKYLAMKNISRNTISLAEQEFELGKLNILLEAEIT
                     AYDYHFRKRLLALYEKRSNDCSVLLEAWKKKLEEGETTINEYNRIALELSAVRLRKSN
                     IEAEIKSLEQKLLYLAGGKINFPEAIEYPEDLTIEPDSLIKIKLLKHPAFLIPVREYE
                     LSFSEMSLGRSSGLPELMAGYSSEILPSETYTGPVIGISIPLWASSGKLKSARARVYQ
                     AEASMNSTIEKLKSEIKREYAVMESLKLAINDLQGILKSSGGTAGPEKALLEGEITLT
                     EYFSLLGIIYDTEEKLIETENAYCRSLAVLNDHQLQEMFK"
     gene            complement(4149..>4729)
                     /locus_tag="PLG42_08320"
     CDS             complement(4149..>4729)
                     /locus_tag="PLG42_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU31250.1"
                     /translation="LENATKMAEDHAKLGVKYCIQPWIVEEARTTIASYQKMAADWNE
                     IGRILKGYGIMFGYHNHNFEFAEVEGKIPYFDVMLAELDPNYVTLELDVFWVSKAGHN
                     PVDIINKYPGRFELFHLKDMFTKQEPFFTTVGVTDFAPVGEGVIDFKSIIATKETAGM
                     KYMIVEQDLTADGDCFGAIQKSITNLTTKILV"
BASE COUNT         1411 a         1026 c         1054 g         1238 t
ORIGIN      
        1 actgatattt acagggcaag gcagactgtc accgagaggc tttaccaggt gtcggaacag
       61 ctgcctgcag atgccggcaa accggttctg gcaccgcaat cgtcactgct cggtgaaatg
      121 atgataattg cagttacatc cgactccctg aaccccatgg acctccggac ctttgccgga
      181 tggagcatca cacccagact cctgtccatt cccggcgtgg cacaggtgca tgttatagga
      241 ggcgatgaaa aagaatatca gattcttgct gatcccctga aaatgaacta ttacaacgta
      301 tcaatagatg aactgctgga agcatgcaga aacataaatg aaaacagtcc gggaggattc
      361 ataaaccagc acggaaataa atatatcgtc agaggcatag gcagaaccag tgatcccggt
      421 gacattgccg caggtgtcat taaaacggtt aacaatcagc ctgttaaaat cggcaatgtg
      481 gctgaggtca ccataggctc ttcaccaaaa ataggtacgg cttcatacaa tggacacaat
      541 gcagtactta ttactgttac acggcaacca catgcaaact cagtaaaact tgccggtgaa
      601 ataatcagag taacagatga aatacaaaaa aatgcaggga acaagattgt aattcacaca
      661 ggggtttaca accaggctgc ttttataaaa actgctatta acaatgtatt gaaggcactt
      721 accgaaggag gtatttttgt tatcctgatt ctcttcattt ttcttttcaa cttccgcaca
      781 acctttatct cggttcttgc aatccctttt tcattgcttg tgtcggtcct cacactgagg
      841 ctgctgggct ataccatcaa cacaatgagt ctgggaggta tggcaatagc catcggttca
      901 cttgtggatg atgccattat tgatgttgag aattcatgga aacagctgag gttaaacgca
      961 ctgaagcacg gtacagaaaa agagcatcca cttagagtaa tactcagggc atcggttgag
     1021 atgaggtcat ctattgtaaa tgccacagcg gtaataattg taacatttct tcctcttttt
     1081 cttctggaag gcttcgaagg gagaatgctc aagccgcttg gaattgcatt tattgtttca
     1141 ctttttgcct caatggttat agccattact gtcactccgg ttctttgcag ctatctgttg
     1201 actaacgaaa agagattgca taagcttgcc cgaggtgcat gggtagaaag aaatctcacc
     1261 tcagcctata aaaaagtgct tgagcctgca ctgaagagac ccggaattat catagcctct
     1321 gcagcaacac ttcttgctgt gtcagtgata atgcttgtca cctccggaag ctcttttctt
     1381 ccgcccttca atgaaggagc acttacggta aatatcggac ttatgccagg cacttccatc
     1441 gaggaatcgg ataaaattgg acgaatggca gaagaaatat tactgggaat tcccgaagta
     1501 aaagcagtat cgcgcaaaac aggaagggca gaacttgcag aacactcttt cggagagaat
     1561 ttttctgagc ttgatgttcc ctttgagctg cgaaaaagga gcagggccga cttctttgct
     1621 gatgttcgca gcagacttgc aaaactacca ggtgccagcg tggaggttgg acagccgata
     1681 acacatcgta tcgataacat gctttcgggt tcaaaggcaa acattgccat caaaattttc
     1741 ggagatgatc taaatgttct atacaacctt gcacaggaaa taaagcatga aatatctgat
     1801 ataaaaggca ttgcagacct aacggttgaa cagcaggttg aaacacccca gctgaagatc
     1861 aaaccaaggc gcgagttact tgcaatgtat ggtatacctg caggaaagtt tacatctttt
     1921 atcagatgta tgctgggagg tgaaacgatt tcggaggtat atgaggagga gaagcgtttc
     1981 ccgatggttg tgagatacaa cgacgaaaca agagcatcta ttgaaggcat aaagagctca
     2041 ctcatcgaca cacatgatgg aaggaagata cctcttacta tggttgcaga aattgaatca
     2101 tcatcgggtc cgtatagcat caacagggaa aatgtgcaac gcagaatagt catctcggta
     2161 aatgtttcgg gtagtgacca gggaacagta gcaaagaaca tccgtaacag gattgaaaag
     2221 aatatcacac tgcctgtaag ctaccgcatt gagtacagcg ggcagtttga aaacgcagcc
     2281 cgtgctgcac gaaggcttct tctggcttca gttgcagcat tgattgctgt ttttatgata
     2341 ctttacaatg aattcagaaa aaaatcactg gcgctgataa tacttctcaa cctgcccctc
     2401 gcattgatag gaggtttctt cgctgtaaaa ctctcttcag gcgttgtgag cattcctgcc
     2461 atcattggtt tcataaccct cactggcatt gcaacccgca acgggatact gcttgtttca
     2521 agatacaccc acctgaaaga agaaggtgta cctattaagg agaggattat aagaggttct
     2581 gctgaccgtc ttaaccctat actcatgaca gccctgacag cagcctttgc cctcatcccc
     2641 cttgctctgg gaggtgataa agccggcaac gaaattcaaa gccctatggc tgttgtcata
     2701 ctgggcggac tcctgagttc aacactgcta aatctatttg ttgtgcctgc agtctattct
     2761 gttgccgaaa aaaatagttc acaagaaaat gctaaatcag aataatatgt cggttaaaac
     2821 tataacgaaa tttttagtta tattaatcag cattccgtta gccggtcagg atttgaattc
     2881 ctttctcaga gaggttgctg agaacaatcc tgagataata gcataccgta aaatgcttga
     2941 agcccgcaag gctgaagccc ggacaaatct atatccttcc ggacctgaag cttcatttgg
     3001 ctatatgcct ggaagcggtt cgactgccgg aacaaagaag acctggtcgg taagccagaa
     3061 atttgatttt cctgcaaaat atctggcaat gaaaaatata agccggaata ccatatctct
     3121 tgccgaacag gaattcgaac tcggaaaact taatatactg cttgaagcag aaataactgc
     3181 atatgattat catttcagga aaagacttct ggcactctac gagaaacgca gtaatgattg
     3241 ttcagtttta ctggaagcat ggaagaaaaa acttgaagag ggtgagacta ccataaacga
     3301 atataacaga attgcccttg agctgtcggc agtcagattg agaaagagca atattgaggc
     3361 tgagataaag tcgcttgagc aaaaactttt atacctcgcc ggtggcaaaa ttaactttcc
     3421 tgaagcaatc gaatatcctg aagatctaac catcgagcct gattctctga taaagattaa
     3481 gctgttaaaa catcctgcct ttctcattcc tgtcagggag tatgagctca gcttcagtga
     3541 gatgtcgctc ggacgctcat ccggccttcc cgaattgatg gccgggtatt cctcagaaat
     3601 tttgccatcg gaaacatata ccggtccggt tataggcatt tcaatacctc tatgggcaag
     3661 ttcgggtaag ctgaaaagtg caagggcaag ggtttatcag gctgaggctt cgatgaactc
     3721 aacaattgaa aaacttaagt cggagataaa aagggaatat gcagtaatgg aatcccttaa
     3781 actggcaata aacgatttac agggcatcct taaatcttcg ggcggtactg caggaccaga
     3841 aaaagcactg cttgaaggtg agattactct gacggagtat ttttcgctgc tgggtataat
     3901 ttatgacact gaagagaagc tgatagaaac agaaaatgcc tactgtagat cgcttgcagt
     3961 gctgaacgac caccagctgc aggaaatgtt caaataattt ttaccggatt tttgaaataa
     4021 acagcacacc gaccacacgg attgaattac tccgggcatt tgccggtgaa atccatcatg
     4081 tccgtatcat cagtgtgcta atagagaacc ggttgtttga atgaaccggg tcgtaaaccg
     4141 aaattattct aaaccagaat ctttgtggtc aggtttgtaa tactcttttg aatagcacca
     4201 aagcagtcgc cgtcagcagt cagatcctgc tcaacaatca tgtatttcat tcctgctgtc
     4261 tcctttgtgg caatgattga cttaaaatca attacgcctt caccaaccgg tgcaaagtcg
     4321 gttacaccaa cggtggtaaa gaaaggctcc tgtttggtga acatatcttt cagatggaac
     4381 agttcgaatc ggcctggata cttattaata atgtcaaccg ggttatgccc tgcctttgaa
     4441 acccagaata catcaagttc cagggtaaca taatttggat cgagttcggc aagcatcaca
     4501 tcgaaatagg gaattttgcc ttccacttcg gcaaactcaa aattgtggtt gtgataaccg
     4561 aacattatcc cgtacccctt cagaatcctg ccaatttcgt tccagtcggc agccatcttc
     4621 tgatagctgg caattgtagt ccgggcttct tcaacaatcc acggctggat gcagtatttc
     4681 accccgagtt tggcatggtc ttctgccatc tttgtggcat tctcaagtg
//
LOCUS       DAOSNB010000203         4700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18247_length_4700_cov_4.265016, whole genome shotgun sequence.
ACCESSION   DAOSNB010000203 DAOSNB010000000
VERSION     DAOSNB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_4700_cov_4.265016"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3095)
                     /locus_tag="PLG42_08325"
     CDS             complement(<1..3095)
                     /locus_tag="PLG42_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU31251.1"
                     /translation="MKKYLLFAVAIIMFSGCMKYNSYEGVPFTEKEPRDWENQELTGL
                     NNEKPHATLISYPDEASALAGVKENSPNYICLDGIWKFHLSKTPEERPYWFFKNDFDT
                     RGWDDIEVPSNWQVMGYDDPPIYSNIPYPFPANPPKIPHDMNPVGSYKRTFKIPSAWK
                     DKEIFLHFGAVSSFFNVWVNEQFVGFGKDSKTPVEFNITPYIKKGRNTLAVEVYRWSD
                     GSYLEDQDFWRLSGIQRSVYLHARPVVYINDFFAIAGLDSTYSAGKLNLDVVLASRPE
                     LSSTYNVVASLFDGERKIYEETLKTTLSEGKSFVTFKNDFPGIKKWSAETPNLYSLVI
                     SLKDENGSNIESVSTKIGFRTVEIKNSQLLVNGVPVYLKGVNLHEHHDVKGHVIDRET
                     ILKDIRLMKSYNINAVRTSHYPQQELWYEMCDRYGLYLIDEANIESHGMGYNKDVTLA
                     DRPEWYAAHIDRIIRMVERDKNHPSVIIWSMGNEAGDGHNFLNAYKWIKERDKTRPVQ
                     YERAEKITNAPERHTDIWCPMYARIEYLENYAKDEKNDRPLIMCEYAHAMGNSVGNLQ
                     DYWDVIEKYPKLQGGFIWDWVDQGLVKTSETGEKFWAYGGDFGPEGITSDGNFCINGL
                     VWPDRTPHPSLHEVKKVYQYIGFEPEDITKGLVKIKNKYDFTDLSEFNFEWEIAADGA
                     VVLSGSFRIPTLPPHRETVVSLPVSQLKPLEATEYFLNIRASRSAPWGIIPEGHVYAA
                     EQIALPVSLPATATLPRDPLSVLQTEEKNSKLFVRGENLEVAFDLGKGIMESLKYKGR
                     EHLLKGPQPDFWRPPTDNDYGYDMDRKLGIWKKAGEGAVVKKADISQPEMDKVVIAFR
                     YDINFAEGVKIADYSSVYTIYSTGDIIVNNRFVKAGKNIPEIPRMGMQMQLPESFSNL
                     KWFGRGPHENYADRKTSAFVNLYESTVADQYVPYIRPQENGYKTDTRWLTLTDDAGNG
                     LLIQGMPLICFAALHNIHDDFESPGKLSAYRKDAKTANRHTTDVKPRDLVNLNVDLAQ
                     MG"
     gene            complement(3231..4535)
                     /locus_tag="PLG42_08330"
     CDS             complement(3231..4535)
                     /locus_tag="PLG42_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOU31252.1"
                     /translation="MAMKQFDEILREKVKEVFSNYNADHLAEKGWQSFVARRRKKFPG
                     VLFIMPLWAKAASLAILISVGTLLTYKIVNRQNEAPITGIRSEEVEKQKESIAPETSV
                     EAKTEVAEKLSASGDKSYAEKIIDRQVSVNSVITVGINGEIVSKPAEFAHNEIFKIAD
                     NEPIAGLPDTGKIQKPDARTESPLNEPDSVLMAEALKNYTETGVSEEEKKGKSHKNKS
                     SVIAGVSGMMAVIDNALATTPGVSAGFYYEYQFTRRIAIRPGVAIAMHSSPVENRTGR
                     TNLNYAAPLYDGTTGTTDSYEASLRLVALELPVNMVFNVIAKEKSSLFLSAGASTLIC
                     LSQKFTGTFVNSYTKTVTNSVTGEMMTYSNYSTVEVEKTERAFSHTDYFGLANISAGY
                     SLPFGRGSLLIEPYVQMPLSGITSLDVKIYYSGISLKLKFGK"
     gene            complement(4516..>4700)
                     /locus_tag="PLG42_08335"
     CDS             complement(4516..>4700)
                     /locus_tag="PLG42_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOU31253.1"
                     /translation="EHYRIIFNLYEIEGYSHDEIAGMLGISASTSRANLARAKKMLRE
                     LYIKNHISVRHGNEAV"
BASE COUNT         1253 a         1066 c          905 g         1476 t
ORIGIN      
        1 cccatctgtg caagatcaac attcaggttt acaagatccc ggggtttcac atcggttgtg
       61 tgcctgtttg ccgtttttgc atctttcctg taagccgata gcttgcccgg tgattcaaaa
      121 tcgtcgtgga tattatgaag tgccgcaaag catataagcg gcattccctg gatcagtaaa
      181 ccgttgccgg catcatcagt gagggtaagc cagcgggtat ctgttttgta gccattctcc
      241 tgaggccgta tataaggcac atactgatcg gctaccgtgc tttcatatag attaacaaat
      301 gccgaagtct tccggtcagc atagttttca tgagggccac gaccgaacca tttcagattt
      361 gaaaacgact caggcaactg catctgcatt cccatcctcg gaatctccgg aatatttttc
      421 ccggccttca caaaccgatt atttactatt atgtcgcctg tgctataaat tgtataaaca
      481 gaagaataat cagcaatttt tactccttcg gcaaagttga tatcatatct gaatgcaata
      541 accactttat ccatttcagg ctgtgaaata tctgcctttt taacaacggc tccctcccct
      601 gctttcttcc agatgccaag cttccggtcc atgtcatatc cgtaatcatt atctgttggc
      661 ggtctccaga aatcgggttg aggacctttc agcaaatgct ccctgccttt atatttgagc
      721 gattccataa ttcctttgcc aagatcaaat gcaacctcaa ggttttcgcc tcttacgaaa
      781 agctttgaat tcttttcttc ggtctgaaga acagataacg ggtcacgtgg aagggttgca
      841 gttgcaggca aagacacagg aagagcaatc tgttcagcag catacacatg tccttcggga
      901 attatgcccc atggtgctga tcttgatgct ctaatattca gaaaatattc agtagcctct
      961 aaaggcttta gctgtgatac cggcagtgag acgacagttt cacggtgggg cggaagagtc
     1021 ggaatccgga aactgcctga aagaacaacg gctccatcgg cagctatctc ccattcaaag
     1081 ttgaattccg acaggtcagt aaaatcatat ttgtttttga tctttaccag tccttttgta
     1141 atatcttctg gttcgaatcc aatatattga tacacttttt taacctcatg gagcgaagga
     1201 tgaggcgttc tgtcgggcca aaccagcccg ttaatgcaga aattgccatc acttgtaata
     1261 ccttccgggc caaagtctcc tccgtaagcc cagaattttt caccggtttc gctggtcttt
     1321 acaagaccct gatcgaccca gtcccagata aatccaccct gcaatttggg gtatttctcg
     1381 ataacatccc agtaatcctg aaggttacca acactattac ccatagcatg ggcatattca
     1441 cacattatca atggcctgtc gttcttttca tcttttgcat aattttcaag gtactcaatc
     1501 cgggcataca taggacacca gatatcggta tgcctctcag gagcatttgt tattttttca
     1561 gcccgctcgt attgaacagg cctcgtttta tctctttcct ttatccactt atacgcattc
     1621 aggaaattat gcccgtcacc tgcttcgttt cccatcgacc agattattac cgatggatga
     1681 ttcttgtctc gctccaccat ccgtataatt ctgtcaatat gagctgcata ccactccggc
     1741 ctgtcagcca gtgtaacatc cttgttatag cccattccat gcgactcaat attggcttca
     1801 tcaatgaggt aaaggccata cctgtcgcac atctcgtacc acagctcctg ctgcggatag
     1861 tgagatgtcc tgacggcatt aatgttatag ctcttcatca gccttatatc cttaagtatt
     1921 gtttccctgt caataacatg acccttcaca tcatgatgct cgtggagatt aacccctttc
     1981 aaataaacag gtactccgtt aaccagcagc tgcgaatttt ttatttcaac tgttctgaaa
     2041 ccaatctttg tgcttacgct ttcaatgttg gatccgtttt catccttaag gcttattacc
     2101 agtgaataga ggttgggcgt ctcagcagac catttcttga ttccgggaaa atcatttttg
     2161 aaggttacga aagatttccc ttcagaaaga gtggttttta atgtctcttc ataaatttta
     2221 cgctctccgt cgaaaagtga tgctacaaca ttgtatgttg agcttaattc aggcctgcta
     2281 gccagaacca catcgaggtt gagttttcct gcagaataag ttgaatcaag acccgctatt
     2341 gcaaaaaagt catttatata aacaacagga cgggcatgca ggtaaacact tcgctggata
     2401 ccgcttaacc gccagaaatc ctgatcttcg agatagcttc cgtcactcca gcgataaacc
     2461 tcaacggcaa gtgtgtttct tcctttttta atatatggag tgatattgaa ttcaacaggt
     2521 gttttactgt ctttaccaaa gcccacaaac tgttcattta cccagacatt gaaaaacgaa
     2581 ctcactgcgc caaagtgcag gaagatctcc ttatccttcc atgccgaagg tattttgaag
     2641 gttctcttgt aagaacccac agggttcata tcatggggta ttttaggcgg attggctggg
     2701 aaaggatatg gaatattcga atagattgga ggatcgtcat atcccattac ctgccagttt
     2761 gaaggaactt cgatgtcatc ccagccacgg gtatcaaaat cattcttaaa aaaccagtat
     2821 ggcctctctt ccggagtttt tgaaagatga aatttccata tcccgtcaag gcatatataa
     2881 ttgggggaat tttcttttac acctgccaaa gcagaggctt catcaggata actgatgaga
     2941 gttgcatggg gcttttcatt atttaaacca gtcagttcct ggttttccca gtcacgaggt
     3001 tctttttcgg tgaagggaac gccctcataa ctattgtatt tcatacaacc tgaaaacatt
     3061 attattgcga cagcaaacaa caaatatttt ttcattgata ttcttttttg ttaaaaaatt
     3121 aaatgtcaat atgttgtaaa tataacaaat ttatcatttt taccggtaca tccgtacagg
     3181 attcctgtat aaattttaat tggttgacaa accgggtgat gaaacgattg ctatttcccg
     3241 aatttcagtt ttaacgatat gccgctgtaa tagattttta catcgaggct ggtaataccg
     3301 gaaagcggca tctgaacgta aggttctatt aaaagactgc ctctaccaaa aggcagggag
     3361 tatccggccg aaatattggc aagaccgaaa taatcagtat ggctgaaagc tctttcggtt
     3421 ttctcgactt caacagttga gtagtttgaa tatgtcatca tttcccctgt aacagagtta
     3481 gtaacagttt ttgtatagga gttcacaaat gtgcctgtaa atttctgact aagacatatc
     3541 agggtcgaag cacctgctga caggaacaga gaagactttt cttttgctat aacattgaat
     3601 accatattaa cgggcaactc aagggcaaca agcctgagac ttgcctcata ggagtcggtg
     3661 gtacctgttg ttccgtcgta gagtggagcg gcataattta gatttgttct gcctgttctg
     3721 ttctcgacag gagaggaatg catagctatt gccactccag gtcttatggc aatcctgcgt
     3781 gtgaactgat actcataata aaagcctgcc gatactccgg gggttgtagc taatgcatta
     3841 tctataactg ccatcatacc cgacactccg gcaattaccg acgatttatt tttatgagat
     3901 tttccctttt tttcttcttc cgaaacacct gtttccgtat aattttttaa tgcttcagcc
     3961 ataaggacac tatcaggctc attcaacggt gactccgttc ttgcatccgg cttctgaatt
     4021 ttgccggtat cgggaagtcc cgcaataggc tcattatcag caattttaaa tatctcatta
     4081 tgagcgaatt cagcgggttt cgaaactatt tcaccattta ttcctacagt tatcactgaa
     4141 ttaacagaga cctgtctgtc tataattttt tcagcataac ttttatctcc agaagctgat
     4201 aatttttcag caacctcagt tttggcttct acacttgttt caggcgcaat tgattctttt
     4261 tgtttttcaa cttcttcact tcggattccg gttataggtg cttcgttttg cctgttaaca
     4321 attttgtatg tcaataaagt accgactgat attaaaattg ccaatgaagc tgcttttgcc
     4381 cataaaggca tgatgaaaag aacgccggga aatttcttcc tgcgacgggc aacaaatgat
     4441 tgccaccctt tttcggcaag atgatcagcg ttgtaattgc tgaacacctc cttgaccttt
     4501 tcccttaata tttcatcaaa ctgcttcatt gccatgtctg actgaaatat gatttttaat
     4561 gtaaagttcc cgtagcatct tctttgctct tgcaagattt gctcttgagg ttgaagccga
     4621 tattcccagc atccctgcta tttcatcatg agaatatcct tcaatttcat agagattaaa
     4681 tattattctg taatgttccg
//
LOCUS       DAOSNB010000204         4649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18510_length_4649_cov_3.717240, whole genome shotgun sequence.
ACCESSION   DAOSNB010000204 DAOSNB010000000
VERSION     DAOSNB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18510_length_4649_cov_3.717240"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1132
                     /locus_tag="PLG42_08340"
     CDS             89..1132
                     /locus_tag="PLG42_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU31254.1"
                     /translation="MKNEKEITIYDIAAELGLSPSTVSRALKDHPGISKETKQQIKET
                     ARRMGYRFNKFASNLRLKRTNTIGVVVPKLNSYFMATAITGIERIASKYNYNLIIANS
                     LENEKKEAEGVATLYNSRVDGLIVSLAYNTSKIKHFDIFLKKGIPIVFFDRVFECKGC
                     VRVIIDNFKAGYEAVSHLICQGCKRIIHVGGNLKRNVYNDRLLGYRKALEDNGINYDE
                     SLVIINDLTERAGEEVVRKILRMKKPPDAVFTANDTTAVSIILNLQKEGISVPGDIAV
                     VGFNNEPISKVIHPNLTTVNYPAYEVGEMAANVLIDRLNSSRDVFPSTIVLEHNLIIR
                     ESSLKNGTIGKKG"
     gene            1405..3573
                     /locus_tag="PLG42_08345"
     CDS             1405..3573
                     /locus_tag="PLG42_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOU31255.1"
                     /translation="MLQKILPVAISLFLSTALPACPETGYRLWLRYDKISDPVLLQNY
                     KSSITGYVFVGNSPTITVTRNELKIALKGLLDCEIPELASPEKIKKKPFILAGTPSDS
                     KIIASLIPLNDLAKTDNEGFIIKTVRFHKANIIIITGNTDMGVLYGTFHFLRLLQTHS
                     NITSLNILDTPKIKIRILNHWDNLNRTVERGYAGLSLWDWNTLPDSLTERYTDYARAN
                     ASVGINGTVLTNVNANALVLTPEYLKKVAALANVFRPYGIKVYLTARFSAPVEIGGLK
                     TADPLDPEVASWWKRKVAEIYSLIPDFGGFTVKANSEGQPGPQNYNRSHADGANMLAR
                     ALAPYGGIVMWRAFVYDDRVPEDRAKQAYNEFVPLDGKFMNNVLIQVKNGPVDFQPRE
                     PFHPLFGAMPHTNVMPEFQITQEYLGASVHLVYLAPLFEECLKSDTYARGKESYVASI
                     IDGTLFNNTLTAMAGVSNIGNVENWTGHPFGQANWYAFGRLAWNPYENSETIAEEWIK
                     MTFSNDQKCVEAIKKIMMNSRENAVNYMTPLGLHHIMYAGHHYGPGPWVSRGRQDWTS
                     VYYHRADTLGIGFDRTTSGSNAVSQYFSPVKEIFNNPETCPENLLLWFHHLPWDYKMK
                     SGRTLWEELCFKYYEGVDAVRQMRKEWQELKYCVDNERFEQVSELMAKQERDAKIWRD
                     GCVLYFQTFSRRPIPEGLEKPEHPLEYYINHKYTDIPGIR"
     gene            3686..>4649
                     /locus_tag="PLG42_08350"
     CDS             3686..>4649
                     /locus_tag="PLG42_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU31256.1"
                     /translation="MLLLGIDAGSSSVKASVLDGETGECLASAFMPETEMEIIAEKPG
                     WAEQDPSMWWDNLKLAVGKCTAVIGKKADKIGAIGISYQMHGLVVVDRELKPLRRSII
                     WCDSRAVSYGNKAFEELGKEFCLSHLLNSPGNFTAAKLAWVKENEPHLFGKIYKIMLP
                     GDYLALKLTGELKTSFTGLSEGIFWDFERNEINSELLKFFGFDRSLFPEAVPSFSIQG
                     YLKKDVAAELNLPAGIPVAYRAGDQPNNALSLNVLSPGEVAATAGTSGVIYGVTDQKK
                     YDPYSRVNTFLHVNHTLQSRRLGVLLCINGTGILNSWIKKNSGNG"
BASE COUNT         1517 a          973 c          955 g         1204 t
ORIGIN      
        1 aagcaaccga ttgcattttt attatgcttt tgttttctta aaatgatttt ttgtattaat
       61 ttgcagtatt atatataatg ttttatagat gaaaaatgag aaggagataa ctatatatga
      121 cattgctgca gagttaggat tatctccgtc gacagttagc agagcattaa aagaccaccc
      181 gggaatcagt aaggaaacaa agcagcagat taaagaaacg gcccggcgga tgggttaccg
      241 gttcaataaa tttgccagca atctgaggct aaaaagaaca aatactattg gggttgttgt
      301 cccaaaacta aacagttatt ttatggcgac agcaataacc ggaatagaaa ggattgcaag
      361 caagtataat tataatctga taattgcaaa ttctcttgaa aatgaaaaaa aggaggctga
      421 aggagtagca acactttata atagtcgggt tgatggtctg attgtatcgc tggcatacaa
      481 cacttcgaaa ataaaacact ttgacatatt tctaaaaaaa ggtattccca tagttttttt
      541 cgacagggta tttgaatgca aaggctgcgt cagagtaatc attgacaatt ttaaagcagg
      601 ttatgaagcg gtttctcatc tgatttgtca gggttgcaaa aggataatac atgtgggtgg
      661 taatctgaaa agaaacgttt ataatgacag gttgctcgga tatcgcaaag cgctggaaga
      721 caatggtatt aattatgatg agtcgcttgt aataattaat gatctcacag agagggcagg
      781 tgaggaggtg gtgagaaaga ttctgcgtat gaaaaaacca cccgatgctg tatttacggc
      841 aaatgataca acggctgttt cgataatcct taatcttcaa aaagaaggta tttcagttcc
      901 aggcgacatt gccgttgttg gatttaataa tgaaccgata agcaaagtga ttcatcctaa
      961 tctgacaaca gttaattatc ctgcatatga agttggagaa atggcagcga atgtattgat
     1021 tgacagactt aactcttcga gagatgtttt tccttcgacc attgttctgg aacataatct
     1081 cattataagg gaatcgtcat tgaaaaacgg aacgataggg aagaagggct gataatagtt
     1141 gccagaatgc gcaaattgaa ttgaaacgag caaaagggtt ttttatcaaa tgataaaaag
     1201 ttctaaataa taaatttttt taaatagtac cgattgccaa ttaataaaca tttttaaata
     1261 ataataaata tcaaatagta aagtttttta aatagtaaat gttatcaaat agtaaaaagt
     1321 atcaattgat aaaaatttct ttttgataaa attttttaaa taataaaaat tctaaatatt
     1381 gaaattaata ctttatatat gagtatgtta caaaaaatat taccagtcgc catatctctt
     1441 ttcttatcaa ctgctttacc agcctgcccc gaaaccggct accggctctg gcttagatac
     1501 gacaaaatat ctgacccggt actgcttcaa aactataaat catccattac cggctacgta
     1561 ttcgtgggta acagccccac gattaccgtc accagaaatg aactcaaaat agcactcaaa
     1621 ggcctgctcg actgcgagat ccccgaactt gcatcacccg aaaaaattaa aaagaaaccc
     1681 tttatactcg ccggtacacc ctccgattca aaaatcatcg cttccctgat ccctctcaac
     1741 gacctcgcga aaacagacaa cgagggcttt ataataaaaa ccgtcagatt ccataaggca
     1801 aatattatta tcataaccgg aaataccgac atgggagtac tctacggcac ctttcacttc
     1861 ctccgccttc tccagactca ctccaacatc acttcactta atatcctcga cacccctaaa
     1921 ataaaaatac gcattcttaa ccactgggat aacctcaacc gtaccgtcga aagaggttat
     1981 gccggccttt ctctatggga ctggaatacc ctgcccgata gcctgacaga gagatacacc
     2041 gattacgccc gcgccaacgc ttctgtagga ataaatggta cagtacttac aaatgtcaac
     2101 gctaacgccc tcgtactaac acctgaatac cttaaaaaag tggctgccct ggcaaatgtt
     2161 ttcagacctt atggcataaa agtctacctc accgcacgct tcagcgcacc tgtcgaaatt
     2221 ggcggactta aaacagccga cccgctcgac ccggaagttg ccagctggtg gaagagaaaa
     2281 gtcgcagaga tctacagcct tattcccgat ttcggaggtt ttacagtaaa agcaaattcc
     2341 gaaggacaac ccggccccca gaactacaac cgctctcatg ccgacggcgc aaatatgctc
     2401 gccagagctc ttgcccctta cggcggtatt gttatgtgga gagcatttgt atatgacgac
     2461 agagtgcctg aagaccgcgc aaaacaggca tataacgagt tcgtcccact cgacggaaaa
     2521 ttcatgaata acgtattaat tcaggtgaaa aacggaccgg tcgatttcca gcctcgagaa
     2581 ccattccacc cactcttcgg cgcaatgccc cataccaacg tgatgccaga attccagata
     2641 acacaggaat acctcggagc ttcagtacat cttgtatacc ttgcacccct cttcgaagaa
     2701 tgccttaaat cagatactta cgccagagga aaagagtcgt acgttgcttc aatcatcgat
     2761 ggaacccttt ttaacaacac acttacagct atggccggcg tttccaatat cggaaatgtc
     2821 gaaaactgga caggccaccc cttcggccag gcaaactggt acgccttcgg acggctcgca
     2881 tggaacccat acgaaaattc ggaaacaatt gccgaagaat ggattaagat gacattttcc
     2941 aacgatcaga aatgtgttga ggcaataaag aaaattatga tgaactcgcg cgaaaacgca
     3001 gttaattata tgacaccact tggactgcac catataatgt acgcaggaca ccattacgga
     3061 ccgggcccgt gggtcagcag gggccggcag gactggacct cagtatacta ccaccgcgcc
     3121 gatacactgg gaataggatt cgacagaaca acttcaggaa gcaatgcagt gtcacaatat
     3181 ttcagcccgg taaaagaaat ctttaacaac cctgaaacat gccccgaaaa cttactcctt
     3241 tggttccatc accttccatg ggattataaa atgaaatcag gcagaacatt atgggaagaa
     3301 ctctgtttta aatattacga aggagtggat gcagtaagac agatgagaaa agagtggcag
     3361 gagttgaaat attgcgtcga taatgaaaga tttgaacagg taagtgagct tatggccaaa
     3421 caggaacgcg acgcaaaaat ctggagagac ggttgtgtgt tgtatttcca gactttttca
     3481 cgcagaccaa ttcctgaagg actcgaaaaa ccagaacatc cgcttgagta ttatattaac
     3541 cacaagtata ccgatatacc gggcatcagg taaataccaa aaacaaaacc aattattaaa
     3601 gataatctgt aaaaaattga ccaaacaagt tatatatttg aggagagttt cagatttcat
     3661 tctgatattt taaagttgta ttgccatgtt attattaggt atagatgccg gaagttcatc
     3721 ggttaaagca tctgttctcg acggggaaac aggagagtgc cttgcgtcag cttttatgcc
     3781 tgaaacagaa atggagataa tagctgaaaa gccgggatgg gcagaacagg atccttctat
     3841 gtggtgggac aaccttaaac ttgcagtggg gaaatgcact gctgtgattg gtaaaaaagc
     3901 cgataaaata ggagcaatag gaatatcgta ccagatgcac ggactggtgg ttgttgacag
     3961 agagcttaaa cctctccgcc gttcgataat atggtgtgat agccgggcag tcagctatgg
     4021 taacaaggca ttcgaagaac tcggaaaaga attttgcctt tcacatcttc ttaattctcc
     4081 cggaaacttt accgctgcca aacttgcatg ggttaaagaa aacgaaccac atctatttgg
     4141 gaaaatatat aaaatcatgc ttccgggcga ttatctggca ctgaagctca ccggcgaact
     4201 taaaacatca tttacaggcc tttcggaagg tattttctgg gacttcgaaa gaaatgaaat
     4261 taacagtgaa ctgctgaaat tttttggttt cgacagatcc ttatttcctg aagctgttcc
     4321 ctctttttcc attcagggct accttaaaaa ggatgtcgct gctgaactga acctcccggc
     4381 aggaatacct gttgcctaca gagccggaga ccagcccaac aatgctctct ctcttaatgt
     4441 cctttccccc ggcgaagttg ccgctactgc gggtacatcc ggggtgatat atggtgtcac
     4501 cgaccagaaa aaatacgatc cttattcaag agttaatacc ttcttgcatg taaaccatac
     4561 acttcagtca agaagactgg gtgtactact ttgtattaac ggaactggta tactcaactc
     4621 atggataaag aagaattcgg gtaacgggt
//
LOCUS       DAOSNB010000205         4624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18655_length_4624_cov_4.928868, whole genome shotgun sequence.
ACCESSION   DAOSNB010000205 DAOSNB010000000
VERSION     DAOSNB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18655_length_4624_cov_4.928868"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLG42_08355"
     CDS             <1..267
                     /locus_tag="PLG42_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HOU31257.1"
                     /translation="TEKKIVAGISTKESWETLTGKWNSVANSVAGKIAGLKPVNTDLD
                     DYLTNKALSGIFLKVEQEEYKIRKDVSARVTPLLQRVFGSLDKK"
     gene            295..1422
                     /locus_tag="PLG42_08360"
     CDS             295..1422
                     /locus_tag="PLG42_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31258.1"
                     /translation="MFERLLFNYEDLEIRISDYANEDILYLLNHTVIGSEGGMRYSLR
                     NIEPKIEGYGDKIRFVSLYKKKQLIGCIGACYRLTGVGRLKAYCSFMRYLAFQSSYQT
                     GMRADGEKRLRDKAEKDGSFKQKILDLFSKPHILNINEVGEKDKHIMYAFVEGTNERS
                     KNLISQAGYELARSFQTVAFSRFSPERHPAATLLSEEEKPVMTKLLTDYYRDYSLFTT
                     EFAFYNNKYYVLKEDGEIKAGVCVIPSEYRVYNIPGVWGWVIMKVLPHVPVYKKLFNP
                     DKFRFLVFEAIYCKEGEEKKLESLFESVCAIEGYNTALTWADDRSQLFEMLKTKVDTG
                     ALGRMLNAKPGLICYRFINFNDEEKDLFYETPAYISAYDFS"
     gene            complement(1445..2350)
                     /locus_tag="PLG42_08365"
     CDS             complement(1445..2350)
                     /locus_tag="PLG42_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOU31259.1"
                     /translation="MKWLKNNNTLIIINPRAGRKRIIEVSERIKKFSNLFDYAVFYDV
                     NEFRNFIRENINNYEIFVAAGGDGTVNSLATELAGNDKTLAVLPVGSGNGFAREMGFR
                     KSIRKLIKDIEYNKTFYTDVLYVNGIPCINVFGLGIDSYVAHDFSNSEKRGFWSYVVA
                     LLKIISSIKPVKVKVDIDGKQIEEEVFMFSVTNTRQFGNHAIITPAAAPNDGKLNVVI
                     ARPFPKIFLPLFAAKLMTGTLKESKYVSYFETDNPVTIETSETRFHIDGEPVKIDSPI
                     TVTIRKNALKVLVTRRNKWMKKKQL"
     gene            complement(2405..3478)
                     /locus_tag="PLG42_08370"
     CDS             complement(2405..3478)
                     /locus_tag="PLG42_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU31260.1"
                     /translation="MDFSLLKKLLPGLIPVFIFIIVDELWGTEAGLYTAVAFGTGEFI
                     FYYLRNKKVDFFIIFDILLLVLLGVVSIVLDNKIFFKLKPALIETILMAIIAFSLWGP
                     KNLILAMSKRYTGELTLPSAAEKAMRANLKIMLTITFFHVLLILYSAKYMSDAAWAFI
                     SGGLFYIIFGLWFAGIFLINTYRKRKYRDEEWFPVVDPDGKITGTAPRSVCHNGKSKL
                     LHPVVHLHLLNREGKIFLQKRSPAKDIQPGKWDTSTGGHVAAGENIKDALKREVLEEI
                     GLKNFNAHFLGKYVWESEIEKELVFSFLCITDEIPKIDSPEITEGRFWSKAEIEKSLG
                     KGIFTPNFEHEYRLLKEKSVIKT"
     gene            complement(3556..4464)
                     /locus_tag="PLG42_08375"
     CDS             complement(3556..4464)
                     /locus_tag="PLG42_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31261.1"
                     /translation="MKNIFYSILVLLTISLLLTEGCRHKKPEEKTPKRTFSTYRSGTG
                     NEAIRAKKEIFYGLLTPTEITTIFNRLGVPHDNSVLNPVSNADLYTSNAKAAINLGIY
                     GVDFGYVKIFGIGQQMIDYILTVREMSNKLGIPEKFLIEPVRKLESGMSDPDSVMALV
                     NKSYYDIENHLRKDGRESTAGLMLMGGWIEAMYITTQLLCDPENPDKEVVEKIAQQKY
                     TLTSLIGFMRNYYDDPVVVYYTKKLIYLRRFYDTFDIYFKKGDLEIDTTRKVLLASGS
                     ETNVTLETLLKIRDYVNKLRTEIVTP"
BASE COUNT         1343 a          864 c          953 g         1464 t
ORIGIN      
        1 accgagaaaa aaatagtggc agggatatca acaaaagaat cgtgggaaac acttaccgga
       61 aaatggaata gtgttgcaaa ttctgttgca ggtaaaatcg caggtttgaa accggtaaat
      121 accgacctgg atgattatct gacaaacaag gcactttcag ggatatttct gaaggttgaa
      181 caggaagaat acaagataag aaaagatgtt tcagcacgcg taactccact tttgcaaaga
      241 gtttttggca gtctggacaa gaaataaaaa ataaaggaaa gtacttcagg tacaatgttt
      301 gagagattat tgttcaatta tgaggacctt gaaataagga tctccgatta tgctaatgaa
      361 gacatcctct atcttcttaa tcatacagtt attggctcgg agggaggaat gaggtattcc
      421 ctccgtaata ttgagcccaa aattgaaggt tacggtgata agataagatt cgtttcgctt
      481 tacaagaaaa aacagcttat aggatgtatt ggtgcctgct accgtctgac aggagtggga
      541 aggctaaagg cttattgttc atttatgagg tacctggctt ttcagagttc atatcaaacc
      601 ggcatgagag cagacgggga gaaaagactt cgtgataaag ctgaaaaaga tgggtctttc
      661 aagcaaaaaa ttctcgacct gtttagcaag cctcatattc tcaacattaa tgaggtaggt
      721 gaaaaagaca agcatattat gtatgctttt gttgaaggaa caaatgaaag gtcaaaaaac
      781 ctgataagcc aggcaggata tgaacttgca aggtcgtttc agactgtagc cttcagccgt
      841 ttttcacccg agcgtcatcc ggcagcaacc ctgctcagcg aggaagagaa gcctgttatg
      901 accaaacttc ttactgatta ttacagggat tattcactct ttaccactga atttgctttt
      961 tataacaaca agtattatgt attgaaagaa gacggtgaga ttaaagctgg agtgtgtgtt
     1021 atcccctcag aataccgtgt gtataatatt ccgggtgttt ggggatgggt aataatgaaa
     1081 gtattacccc acgtaccggt atataaaaaa ctgtttaatc cggataaatt tcggtttctg
     1141 gtatttgaag caatatattg caaggaaggt gaggagaaga aacttgagtc actttttgaa
     1201 tcggtttgtg ccattgaagg gtataacact gctctcacat gggctgacga cagaagccag
     1261 cttttcgaaa tgctgaagac gaaagttgat accggagctc tgggcaggat gcttaatgca
     1321 aaacccggac tgatctgtta ccgttttata aatttcaatg atgaggagaa agacctcttt
     1381 tacgaaactc ctgcatatat ctcggcatat gacttttctt agggatgagt cacaggtgat
     1441 gctgtcataa ctgtttcttt ttcatccatt tattgcggcg ggtaaccagt acctttaaag
     1501 cattttttct tatcgtaact gtaataggac tatcaatttt tactggttca ccatcgatat
     1561 gaaatctggt ttcggaagtt tctattgtga ccggattatc agtttcgaaa tagcttacgt
     1621 attttgattc tttcagtgtt cccgtcatca attttgcggc aaacagaggc agaaaaatct
     1681 tcgggaaagg cctggcaata accacattca atttaccgtc atttggagca gcagcaggtg
     1741 ttattattgc atggttgccg aactgccgtg tgttggttac cgaaaacata aatacctctt
     1801 cttcaatttg tttcccatca atgtcaactt tgaccttaac aggtttaatt gaacttataa
     1861 ttttaagcag ggcaacaaca tagctccaga agcctctttt ttctgagttg gaaaaatcat
     1921 gggccacata actgtctatt cccagcccga aaacgtttat gcaggggatt ccgttaacat
     1981 aaagtacatc ggtgtaaaat gttttattgt attcaatgtc tttaataagc tttctgatac
     2041 tttttctgaa gcccatttct ctggcaaatc cgtttccaga accaaccgga agcactgcaa
     2101 gtgttttgtc attgccggca agctcggtgg caagactgtt tacagtaccg tctcctcctg
     2161 ctgctacgaa aatttcataa ttattaatat tttcccttat gaaattccgg aattcgttca
     2221 catcataaaa aacagcataa tcaaaaaggt tgctgaattt tttaatcctt tcagaaactt
     2281 caataattct tttccgccct gccctgggat taataattat gagagtattg ttatttttca
     2341 gccatttcat ctgttgaccc aaaaacaggt taaatgtact taaagattac cgaataattc
     2401 attttcatgt tttaataacg gatttttctt tcagaaggcg gtactcatgc tcgaaatttg
     2461 gagtgaatat tcctttaccc aggctttttt ctatttcagc ctttgaccag aatcttcctt
     2521 cagttatttc aggagaatct attttgggga tctcatccgt aatacagagg aatgaaaaca
     2581 caagttcttt ttcaatttct gattcccaga catatttccc cagaaaatgt gcgttgaaat
     2641 ttttcaggcc gatttcttct agaacttctc ttttaagcgc atcttttatg ttttcacctg
     2701 ctgcaacatg tcctcctgtt gatgtatccc acttgccggg ttgaatatct tttgccggag
     2761 atcttttctg caggaaaatt ttaccttccc tgttcagaag atgcaggtgc acaaccggat
     2821 ggagcagttt gcttttcccg ttgtggcaaa cactgcgcgg tgctgtacct gttatcttac
     2881 catcagggtc aacaacaggg aaccattctt catctctgta ttttcttttt ctgtatgtat
     2941 ttataagaaa aattcccgcg aaccagagtc cgaaaataat ataaaagagt cctccactga
     3001 taaaagccca ggcagcatcc gacatgtatt ttgcagagta caaaatcagc agtacgtgaa
     3061 aaaatgtaat ggttagcatt atcttgaggt tcgccctcat tgctttctca gctgccgatg
     3121 ggagggtaag ttctccggta tatctcttcg acattgcaag gataaggttt ttcggccccc
     3181 acagcgaaaa ggcaattata gccataagaa tggtttcaat aagtgcaggt ttgagtttga
     3241 agaagatttt attatcaagt actattgaaa caactccgag taaaaccaga agaagaatat
     3301 caaagattat gaagaaatct acctttttgt ttctcaggta atagaatata aactctcctg
     3361 ttccgaaagc aacggctgta tataatcccg cttcagttcc ccataattca tcaacaataa
     3421 tgaaaatgaa aacaggaata agacccggta ataatttttt cagaagagag aaatccataa
     3481 taaaaattga cgggcaaaat tataaaatat ctccggagca ggaaatgttg gaaatattat
     3541 aattgaggtt aaagctcagg gggttacaat ttcagttcgc agtttgttca cgtaatctct
     3601 tattttcaaa agggtttcaa gggttacatt tgtttcacta cctgaagcga gcagcacttt
     3661 gcgtgttgtg tctatttcga ggtctccttt tttgaagtat atatcgaagg tatcgtaaaa
     3721 tcttctcagg tagatgagtt ttttggtata ataaaccacc acaggatcgt cgtagtagtt
     3781 gcgcataaat ccaatgagtg aggtgagagt gtatttttgc tgggcaattt tttctaccac
     3841 ttccttatcc gggttttcag gatcgcaaag cagctgggtg gttatgtaca ttgcttctat
     3901 ccatcctccc atcagcatga ggccggcagt gctttcacgt ccatctttgc ggaggtggtt
     3961 ttcgatatca tagtatgatt tattgacaag tgccatcacc gaatcggggt ctgacattcc
     4021 gctttcaagc ttccgtacgg gttcgataag gaatttttcc ggtatgccaa gcttgttgct
     4081 catttctctt accgtcagga tataatctat catctgctgg cctattccga aaattttgac
     4141 atatccaaag tcgacgccgt atattccaag gttaattgca gctttggcgt tgctggtgta
     4201 aaggtcggcg tttgaaacag gattgagaac tgaattgtcg tgaggtacac ccaggcggtt
     4261 gaatattgtg gtaatttcag tgggagtgag gaggccatag aaaatctcct tttttgctct
     4321 tattgcctca ttgccggttc cgcttctata tgttgaaaaa gttcgtttcg gagttttttc
     4381 ttcaggcttt ttatgcctgc atccttcagt tagcagtaag ctgattgtaa gaagaaccag
     4441 gatggaataa aaaatatttt tcattcaagg tattttttga aatctaattt atgaaaaatt
     4501 tataaaacaa ttctgtctcc cagttttttg gaaatgattg aaagtgtttt ctgcaggtaa
     4561 tcataccttt tctgaagaga gagggtcttt tgtatgtcgc cgttctttga ggcttcattt
     4621 atct
//
LOCUS       DAOSNB010000206         4618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_18692_length_4618_cov_4.216086, whole genome shotgun sequence.
ACCESSION   DAOSNB010000206 DAOSNB010000000
VERSION     DAOSNB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18692_length_4618_cov_4.216086"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLG42_08380"
     CDS             complement(<1..241)
                     /locus_tag="PLG42_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOU31262.1"
                     /translation="MRLIQTIKNIFKIEDLRARLLYTLSIIMIYRLGKYVTLPGVDPA
                     QLENLKSQTSSGIMGLLDMFSGGAFSQASIFALGIM"
     gene            complement(246..692)
                     /gene="rplO"
                     /locus_tag="PLG42_08385"
     CDS             complement(246..692)
                     /gene="rplO"
                     /locus_tag="PLG42_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOU31263.1"
                     /translation="MDLSNLKPAKGSVKARKRIGRGQGSGKGGTSTRGHKGAQQRSGY
                     ERKLGFEGGQMPLQRRVPKYGFTNRNRVEYKAINIGTLQNLAEKLNADKIDIPTLLSA
                     GLISKNDLVKILAKGELNRKLEVHAHAFSKKAIEAIEAKNGTVVKI"
     gene            complement(716..898)
                     /gene="rpmD"
                     /locus_tag="PLG42_08390"
     CDS             complement(716..898)
                     /gene="rpmD"
                     /locus_tag="PLG42_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOU31264.1"
                     /translation="MAKIKITQIRSKNRKPERQKRILAALGIHRLHQSVVHEATPQIL
                     GMVEKIKHLVKTEEVE"
     gene            complement(910..1425)
                     /gene="rpsE"
                     /locus_tag="PLG42_08395"
     CDS             complement(910..1425)
                     /gene="rpsE"
                     /locus_tag="PLG42_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOU31265.1"
                     /translation="MANGIRKVKTTDIELKDRLVSINRVTKVTKGGRTFSFSAIVVVG
                     NEDGIVGYGLGKASEVTAAIAKGIEDAKKNLVRIPVIKGTIPHEQSAKFGGAKVFLKP
                     ASEGTGVKAGGAMRAVLESVGIRNVLAKSKGSSNPHNLVKATIKALLQLRDAYTIAEH
                     RGIPIEKVFNG"
     gene            complement(1433..1792)
                     /gene="rplR"
                     /locus_tag="PLG42_08400"
     CDS             complement(1433..1792)
                     /gene="rplR"
                     /locus_tag="PLG42_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOU31266.1"
                     /translation="MALTKLERRTRIRKRIRKKITGTPERPRLSVFRSNRHIYAQLID
                     DTRGVTLVAACSREKEVADKKGIKKIEQASLVGKLIASKCLSKGIEKVVFDRGGYKYH
                     GRVKSLAEAAREGGLKF"
     gene            complement(1820..2374)
                     /gene="rplF"
                     /locus_tag="PLG42_08405"
     CDS             complement(1820..2374)
                     /gene="rplF"
                     /locus_tag="PLG42_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOU31267.1"
                     /translation="MSRIGKLPINLPKGVTVSVDENNVVHVQGPLGKLQQAVDPDIKV
                     EVTDNQVRVIRPSDSKRHKSLHGLYRALIANMVEGVSKGYKKELEVVGVGYRAEAKGQ
                     NLELSLGYSHDIIFVMPPEVKVETKTEKRSNPIITLTSHDKQLIGQVAAKIRSLRPPE
                     PFKGKGIRFVGEYIRRKAGKSASS"
     gene            complement(2392..2790)
                     /gene="rpsH"
                     /locus_tag="PLG42_08410"
     CDS             complement(2392..2790)
                     /gene="rpsH"
                     /locus_tag="PLG42_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOU31268.1"
                     /translation="MTDPISDFLTRIRNAIMAGHKVVEAPSSKMKIEIARILLEKGYI
                     RAYKVVEGEGPYDVLKIALKYHPRTKMPAIKGIERVSTPGLRKYTNVKDMPRVLNGLG
                     IAIISTSRGLMTDKEAKKENIGGEVICYVY"
     gene            complement(2823..3092)
                     /gene="rpsN"
                     /locus_tag="PLG42_08415"
     CDS             complement(2823..3092)
                     /gene="rpsN"
                     /locus_tag="PLG42_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOU31269.1"
                     /translation="MAKESMKAREVKRAKLAQKYAAKRAQLKAAGDYVGLSRLPRNSN
                     PIRLRNRCKITGRPRGYIRQFGLSRITFREMASKGLIPGVRKASW"
     gene            complement(3099..3650)
                     /gene="rplE"
                     /locus_tag="PLG42_08420"
     CDS             complement(3099..3650)
                     /gene="rplE"
                     /locus_tag="PLG42_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOU31270.1"
                     /translation="MYIPELKKKYKEEIVPALMKEFNYKSVMQVPRLEKIVISQGVGA
                     AIADKKILEAGLKEITDIAGQKAVATYSRKDISNFKLRKNMPIGIMVTLRQDRMYEFL
                     QRLICVALPRIRDFKGINPVLDGRGNYTLGITEQIIFPEIDLDKVVKIMGLQITFVTS
                     ATTDEEALALLKHFGLPFKNLKN"
     gene            complement(3650..3973)
                     /gene="rplX"
                     /locus_tag="PLG42_08425"
     CDS             complement(3650..3973)
                     /gene="rplX"
                     /locus_tag="PLG42_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOU31271.1"
                     /translation="MQKKLHIKKGDTVIVISGESKGQKGRVLEVDRKKMRAIVEGVNM
                     VHKHTKPNAKAPQGGIIKKEAPIHISNLMLIDPTSGKPTRIGRRLNEKNKLVRYSKKS
                     GEEIR"
     gene            complement(3995..4360)
                     /gene="rplN"
                     /locus_tag="PLG42_08430"
     CDS             complement(3995..4360)
                     /gene="rplN"
                     /locus_tag="PLG42_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOU31272.1"
                     /translation="MIQQESRLNVADNTGAKEVLCIRVLGGTKKRYATVGDQIIVTVK
                     SALPSGEAKKGMVSKAVVVRTKKEIRRPDGSYIRFDDNAVVLLTNQDEVRGTRIFGPV
                     ARELRDKYMKIVSLAPEVL"
     gene            complement(4363..4617)
                     /gene="rpsQ"
                     /locus_tag="PLG42_08435"
     CDS             complement(4363..4617)
                     /gene="rpsQ"
                     /locus_tag="PLG42_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOU31273.1"
                     /translation="MERKLRKERTGVVVSNKMDKSIVVAIKRRVKHPVYGKYVTRTKK
                     VMAHDENNECNIGDVVRIAETRPLSKNKNWRLVEIIEKAR"
BASE COUNT         1046 a         1137 c          845 g         1590 t
ORIGIN      
        1 gcataatacc gagggcaaaa atcgaagcct gggaaaaggc gcctcccgaa aacatatcga
       61 gaaggcccat aatacctgac gaagtctggc ttttcagatt ttccagctgg gcagggtcaa
      121 cacccggcag tgtcacatat ttacccagtc tgtaaatcat tattatacta agagtatata
      181 gcaaccgggc cctcaggtcc tctatcttga aaatattctt tatggtctga atcagcctca
      241 tctggttaga tttttactac tgtaccattt tttgcctcta tggcttcaat agcctttttg
      301 ctgaatgcat gggcatgaac ttcgagtttt ctgttaagtt caccctttgc aagtatctta
      361 acaagatcat tcttagaaat gagcccggcc gataaaagag tcggtatatc tatcttatca
      421 gcattaagct tttcagcgag gttttgaagt gtaccaatgt tgatggcttt atattcaaca
      481 cggtttctgt ttgtgaatcc gtatttgggt acccttctct gcagtggcat ctgcccgcct
      541 tcaaaaccaa gttttctttc ataaccggat ctctgctggg ctcctttatg acctctggtt
      601 gaggtgcctc ctttacctga accctgtccg cgtccgattc ttttcctcgc ctttactgag
      661 ccttttgccg gttttaaatt actaagatcc ataccctgtt tgttaaaaat taattttatt
      721 caacttcttc tgttttgacg agatgtttta ttttctcaac cattcctaga atctgaggag
      781 tagcctcatg caccacgctc tggtgtagtc tgtgtattcc aagtgcagca agaatccttt
      841 tctgtctctc aggttttctg ttcttgctcc ttatctgggt tattttgatc tttgccatat
      901 gcagattttc tatccgttaa acactttttc gatggggatt cccctgtgtt cagctattgt
      961 ataggcatcc ctgagctgca gaagtgcttt aatggtagct ttaacaaggt tgtgcgggtt
     1021 tgatgagccc ttcgattttg cgagcacatt tctaatcccg acactttcga gaacagcacg
     1081 catagcacct cctgccttta ctccggtacc ttctgatgca ggcttcagaa aaacctttgc
     1141 tcccccgaat tttgcactct gttcatgcgg tatggtgccc tttattacag gaatcctgac
     1201 aaggttcttc ttggcatctt caattccttt tgctattgcg gctgtcacct cactggcttt
     1261 tccaagcccg taacccacta taccatcttc attgccaaca accactatag ccgaaaaact
     1321 gaatgttcgt ccgcctttag tgactttggt cactctgttg atacttacaa gcctgtcctt
     1381 aagctcgata tccgtggttt ttacttttcg tattccgttt gccataattt cattagaatt
     1441 taagtccgcc ttctcttgca gcttctgcca gtgatttaac tcttccgtga tatttatagc
     1501 ctcctctgtc gaaaactacc ttttcgattc cctttgaaag gcattttgat gcaatcagtt
     1561 ttcctaccag tgaagcctgt tcaattttct taataccctt tttgtcagcc acctcttttt
     1621 cgcgtgaaca ggcggcaaca agtgtcacac ccctggtatc atcaattagc tgggcgtaaa
     1681 tgtgcctgtt gctgcggaaa acagacagtc tcgggcgttc tggtgtcccg gttatctttt
     1741 tccttatcct tttccttatt cttgtccttc tttccagttt tgttaaagcc ataattttaa
     1801 aaattatctg ataatgaaat taagagctgg ccgatttacc agctttcctt ctgatgtatt
     1861 ccccaacaaa ccgtataccc ttgcccttga aaggttcagg tggacgaaga gatcttattt
     1921 tggctgcaac ctgaccaatg agctgcttgt cgtggctcgt cagtgtgata atcgggttgc
     1981 ttctcttttc ggtttttgtc tccaccttaa cctccggtgg cataacgaaa atgatatcgt
     2041 gggaatatcc aaggctcagt tcaagattct gccctttggc ttcagccctg tagcctactc
     2101 caacaacttc aagctctttc ttatatcctt tcgaaacccc ttccaccata tttgcaatca
     2161 gggcacgata gagcccgtga agagatttgt gcctcttcga atcggatggt ctgatgactc
     2221 tgacctgatt atccgtcact tcaactttga tgtctgggtc aacagcctgc tgcagttttc
     2281 caagagggcc ctgcacatga acaacattgt tttcatcaac acttacagtc acccctttgg
     2341 gcagattaat cggtaatttt cctatgcgtg acatctgttt atcctcctct gttaataaac
     2401 atagcaaatc acctcaccgc caatattttc ttttttggct tccttatcgg tcataagacc
     2461 tcttgaggtg gaaataattg caattccaag gccattaagg actcttggca tatcttttac
     2521 attggtatat tttctcaaac ccggtgtact aactctttca atacctttta ttgcaggcat
     2581 tttggttcgc gggtgatact tgagcgcaat tttcagaaca tcatatggtc cttcaccttc
     2641 aacaacctta taggcgcgga tataaccttt ttcaagcagg atccgtgcta tttctatctt
     2701 catctttgat gaaggtgctt ccactacctt gtgtcccgcc attatagcgt ttcttattct
     2761 tgtcaaaaaa tctgaaatgg gatcagtcat catatatcaa attattttca cacaattata
     2821 atttaccagc tggcttttct cactcccggg ataaggcctt ttgatgccat ttccctgaag
     2881 gttattctgc tcagtccaaa ctgtcgtata tatcctcttg gccttccggt gattttgcag
     2941 cggtttctca ggcgaatagg atttgagttt ctgggaagac ggctcaatcc tacataatcg
     3001 cctgctgcct taagctgtgc tctttttgca gcatatttct gagccagttt tgcgcgcttc
     3061 acctcgcggg ccttcatcga ctcttttgcc atatattatc agtttttaag gtttttaaac
     3121 ggtaaaccaa aatgcttaag taacgcaaga gcttcttcat cggtggttgc tgatgttaca
     3181 aaagttatct gaagacccat aatcttcaca accttgtcaa ggtcaatttc cgggaagatt
     3241 atctgctcgg taattcccag tgtatagtta ccgcggccgt caagcaccgg attgattccc
     3301 ttgaaatccc tgatacgcgg gagtgcaaca cagatgagcc tctgaaggaa ctcatacatc
     3361 ctgtcctgcc ggagagtcac cataatgcct atgggcatgt tcttcctcag cttgaagttg
     3421 gatatatctt ttctcgaata agttgccaca gccttctgcc ctgcaatatc ggttatctcc
     3481 ttcaaacctg cttcaagaat cttcttatcg gcaatggctg ctccgacacc ctggctaata
     3541 acaatctttt cgagccttgg tacctgcatc actgacttgt agttgaattc cttcatcagt
     3601 gcaggaacaa tctcttcctt atatttcttt tttaattcag gtatatacat tatctgatct
     3661 cctcccctga ttttttagaa taacgaacaa gtttgttttt ctcattaagc ctccggccga
     3721 tccttgtggg ttttccgctt gtaggatcga taagcataag gttcgaaatg tgaatcgggg
     3781 cttctttctt gattatgccg ccctgaggtg cttttgcatt gggttttgta tgcttgtgca
     3841 ccatattcac accctcaaca atagctctca ttttcttccg gtcaacctca agaacgcggc
     3901 ctttttgtcc tttcgactcg cccgaaatca caattactgt atctcctttt ttaatatgta
     3961 gcttcttctg cataattctt ttattaatat tctgtcataa aacttccgga gcaagggata
     4021 caattttcat gtatttatcg cgcaattctc ttgcaaccgg tccgaagata cgtgttcccc
     4081 ttacctcatc ctgattggta agaagcacaa cggcattatc atcgaatctg atataggagc
     4141 cgtcgggccg gcgtatctcc ttctttgttc ttaccaccac agctttgctg accattccct
     4201 ttttggcttc acctgaaggt aatgcgctct ttaccgtaac aataatctga tctcctacgg
     4261 ttgcatagcg ttttttggta cctcccaata ccctgatgca caatacttcc ttggctccgg
     4321 tattgtcggc aacgttaagt ctcgattcct gctgtatcat agttacctgg ctttttcaat
     4381 tatttcaacc aatctccagt ttttattttt gctgagaggc cttgtctctg caattcttac
     4441 aacatctcca atgttgcatt cattattctc gtcgtgagcc ataactttct ttgttctggt
     4501 aacatatttt ccataaacgg ggtgtttaac ccttcttttt atggccacga caatagattt
     4561 gtccatctta ttgcttacga caacgcctgt tctttctttt cttaatttcc tttccatg
//
LOCUS       DAOSNB010000207         4543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19145_length_4543_cov_4.518939, whole genome shotgun sequence.
ACCESSION   DAOSNB010000207 DAOSNB010000000
VERSION     DAOSNB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19145_length_4543_cov_4.518939"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLG42_08440"
     CDS             <1..871
                     /locus_tag="PLG42_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOU31274.1"
                     /translation="QRVNLESSEIRHLVYLLEENLKPENIYYRYDFFYDNCSTRIRDI
                     FEKIFSDRLVYPVEEKKDMPTFRDKINEYQRYYPWLKAGVDLLLGLPADRKASFRDLM
                     FLPEYLQRNLSASMIKRGDRSEPLLQDTETIIEFEPINVKNPFYSSPLFVFGLILIII
                     ILISTRFRNLTYIKYFDILIFTIFSILSLFLLFFNFITEHAETKMNINFIWINPFIIV
                     CLICLSLNKQCTIWFRLVFWLAVLYIIGVIIFASNIYGTFIPAALILALRSSVYSDFK
                     WNPFGLKNSEIKR"
     gene            990..1985
                     /locus_tag="PLG42_08445"
     CDS             990..1985
                     /locus_tag="PLG42_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOU31275.1"
                     /translation="MEQSVDIKLLNEKIEKESAFVDIIRMEIGKVIIGQRHLIDSLLI
                     GLLANGHILLEGVPGLAKTLAIRSLASIIDAKFSRIQFTPDLLPADLIGTMIYSQKKE
                     EFLVKKGPIFANFILADEINRSPAKVQSALLEAMQERQVTIGETTYKLEEPFLVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVLIGYPSMEEERNIVRENIAKEFPKPNTVLKTSDII
                     RARDVVREVYMDEKIENYILNIVFATREPARFNLQKFSNMISYGASPRASIYLALASK
                     AYAFIRRRGYVIPEDVRAICADVMRHRIGLTYEAEAENINQDHIITEILNTIEVP"
     gene            1997..2422
                     /locus_tag="PLG42_08450"
     CDS             1997..2422
                     /locus_tag="PLG42_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31276.1"
                     /translation="MKSMKAIYLLLLLLLLNSYESRSQNLIGYKIRDIQKYMKENQKS
                     MVFQNLTFNNTFRYLKYVDRAQTQTLFFFLSADSVCRSVRLVCDKELRSQKIAELNSK
                     YKPVGENKWEEEKDGQKYIIDLKDDEWTFNISINIKPVR"
     gene            2434..3297
                     /locus_tag="PLG42_08455"
     CDS             2434..3297
                     /locus_tag="PLG42_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOU31277.1"
                     /translation="MEAAELLKKVRRIEIKTRGLSRHIFAGQYHSAFKGRGIAFSEVR
                     EYQYGDDIRNIDWNVTARFNHPYVKVYEEERELTVMLLIDVSSSGDFGTIKAFKKDVM
                     TEIAAVLSFSAIFNNDKIGVIFFSGKVEKFIRPQKGRKHILRIIRELLDFKPESNLTN
                     LNEPLRYLTNAIKKRCTAFILSDFIAPDFEESLRIAANKHDVVALKIYDPAETSMPDI
                     GLVRIQDPETGYDMWIDTSSGRMRNEYSRWWKNHSENLENVFRRCGVDSAWISTSEDY
                     VIPLVRLFRRR"
     gene            3300..4280
                     /locus_tag="PLG42_08460"
     CDS             3300..4280
                     /locus_tag="PLG42_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31278.1"
                     /translation="MKRIYYILAFLIFNLIQVSEGQEVQISAGFDTSAIFIGDQIKYT
                     VTINKPLDYLLTIPVFKDTLVKNIEILKGPVIDSTSVDNRFVRIRHEYLVTSFDSGFY
                     QVPPVYAEMRTESGIKRYYSDYSWLKVMRVNITPPDTASAIFDIVDPGKVPLTADEII
                     PWIVAILAAGALIWLLLYLWKRLKKPETTIVDNKPKEPAHVIAFRELEKLKEEKLWQK
                     GEIKLYYSRLTEIIRQYLLNRFTVPALEMTTVETLDALRNKGIEEDETFRKLRTILTG
                     GDLVKFARYQPEPTENELHFDYAWDFVKSTMIVSPPPEEEVKAENVKEGI"
     gene            4277..>4543
                     /locus_tag="PLG42_08465"
     CDS             4277..>4543
                     /locus_tag="PLG42_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOU31279.1"
                     /translation="MIRGIVFDSPGFLYLLALLPLMIFLYVIRYNKSSAALRVSSAEA
                     FNNSGKTFRHILRHVLFGMRVAATGLLIIVLARPQATNSYQDVST"
BASE COUNT         1519 a          774 c          905 g         1345 t
ORIGIN      
        1 tcagagggta aatcttgaaa gctcagaaat caggcacttg gtgtatcttc ttgaggaaaa
       61 tctaaaacct gaaaatattt attacaggta cgattttttt tatgataact gctctacgag
      121 gatacgcgac atatttgaaa aaatattttc tgacagactt gtctatcctg ttgaagaaaa
      181 gaaagatatg cccaccttca gagataagat aaatgagtat caacgttatt atccatggtt
      241 aaaggctggt gtggatctgc ttctgggatt gcctgctgac aggaaagctt cattccgcga
      301 cttgatgttt ttaccagaat accttcaacg caacctctca gcttcaatga ttaaaagagg
      361 cgacagaagc gagcccctgc ttcaggatac agaaacaatc atagaatttg aaccaatcaa
      421 tgtaaaaaac ccattttatt ccagtcctct ctttgtgttt gggttaattt taattatcat
      481 aatacttatt tctacaaggt tcagaaattt aacttatatt aaatattttg atattctgat
      541 atttacaatt ttttcaattt tatccttgtt ccttctgttc tttaatttta ttactgaaca
      601 tgccgaaaca aaaatgaata taaatttcat atggattaat ccttttatta tcgtttgtct
      661 tatatgcctg tcgctcaata aacaatgtac aatttggttc aggctggtat tctggcttgc
      721 tgttttatac attataggtg taattatctt tgcatcaaat atttatggta cttttattcc
      781 ggcagcactt attttagcct taagaagttc tgtttattca gattttaaat ggaatccgtt
      841 cggattaaaa aattcagaaa taaagagata aagccattta acaatatttt aacatacctt
      901 aacatcaact ttcagaaata gagggttttt ttgcaaacta taattaacag acaaaaaata
      961 tatttgatta ttgactaaac caaaattata tggaacaatc agttgatata aaactgctta
     1021 atgaaaaaat tgagaaggaa agcgcatttg ttgacataat caggatggaa atcggtaaag
     1081 taatcattgg tcagcgacat cttatcgaca gtttgcttat agggctcctt gccaacggac
     1141 atattctgct ggagggtgtt cccggtctgg caaaaactct cgccataagg tctctggcat
     1201 caattataga tgcaaaattc agccgtatcc aattcacccc tgatcttctt cctgccgatt
     1261 tgatcggaac catgatttat agtcagaaaa aagaagaatt tttagtaaag aaaggaccta
     1321 tatttgccaa tttcattctg gctgatgaga taaacaggtc gccagccaag gttcagagtg
     1381 cacttctgga agcaatgcag gaaagacagg ttacaatcgg agaaacaaca tataagcttg
     1441 aggaaccatt tctggttctt gccacacaaa accctataga acaggaagga acttatcctc
     1501 ttcctgaagc tcaggtagac agattcatgc ttaaggtatt aataggctat ccttcaatgg
     1561 aagaggaaag aaacattgtc cgggagaaca ttgcaaaaga gttccccaaa cccaatacag
     1621 ttctgaaaac ttcagatatt ataagagccc gcgacgttgt gcgcgaagtc tatatggatg
     1681 aaaaaattga aaactatatt cttaacatag tcttcgccac acgtgaacct gccaggttta
     1741 atctacagaa attctcaaac atgatttcat acggagcttc ccctcgtgcg tcaatatatc
     1801 tggcacttgc atcaaaagcc tatgctttta tcaggagaag gggttatgtt atacccgagg
     1861 atgtcagagc aatatgtgct gacgttatga ggcatcgcat cggccttacc tatgaagctg
     1921 aagcagagaa tataaatcag gatcatataa taactgaaat acttaataca attgaagttc
     1981 cataaaaaat ttttacatga agtcgatgaa agccatttat ttattgttgc ttctcttgct
     2041 gttaaattca tatgagagca gaagtcagaa cctgatcgga tataaaatca gggatataca
     2101 gaaatacatg aaagaaaacc agaaatcaat ggtttttcaa aacttaacat ttaataatac
     2161 attcagatat cttaaatatg tcgacagggc acagactcag acgctttttt tctttttgtc
     2221 ggctgactct gtttgcagga gtgtacggct tgtttgtgac aaagagctca ggagccagaa
     2281 aatagctgag cttaattcaa agtataaacc tgtcggtgaa aataaatggg aagaagaaaa
     2341 agatggtcag aaatatatta ttgacctcaa ggatgatgaa tggactttta acataagtat
     2401 caatattaaa ccggtaagat agtacttgca aaggtggaag ctgctgaact attaaagaaa
     2461 gtaagaagga ttgaaattaa aacgagagga ctgagccggc atatatttgc cggtcagtat
     2521 catagtgcat ttaagggcag gggtattgcc tttagcgagg tgcgtgaata ccagtatgga
     2581 gatgatataa gaaatataga ctggaatgtt acggccagat ttaaccatcc gtatgttaaa
     2641 gtatatgagg aagaaagaga actaaccgta atgcttctta ttgacgttag cagttcagga
     2701 gatttcggaa caattaaagc atttaagaag gatgtaatga ctgaaattgc agctgttctt
     2761 tctttctcag caatttttaa taacgataaa attggagtaa tattcttttc gggaaaagtt
     2821 gagaaattta tcagacccca gaaaggcaga aaacatatac tcaggataat aagggaattg
     2881 cttgatttta aacctgaaag taatctgaca aatcttaatg agccattaag gtatctgaca
     2941 aacgcaataa aaaaacgttg tacagcattc attttgtcag actttattgc acctgatttt
     3001 gaagagtctc tgaggattgc ggcaaataaa cacgatgttg tggcactgaa aatttatgat
     3061 ccggcagaaa cttcaatgcc agatattggg cttgtaagga tacaggatcc tgagacaggt
     3121 tacgatatgt ggattgacac ttcatcgggc aggatgcgga atgagtacag cagatggtgg
     3181 aaaaaccatt ccgaaaacct ggaaaatgta ttccgtagat gcggagtgga ctcagcatgg
     3241 atatcaacat ctgaagatta cgtcattccg ctcgtcagat tgttcagaag gagatagaga
     3301 tgaaaagaat ctattacata ctggcttttt taatctttaa tttaatacag gtttccgaag
     3361 gacaggaagt ccagattagt gctggttttg atacatcagc aatttttata ggtgatcaga
     3421 taaaatacac ggttactatc aataagcctc ttgattattt attgacaata cctgttttta
     3481 aagatacact ggttaagaat atcgaaatac tgaaaggacc ggttattgat tcaacatccg
     3541 ttgataacag atttgtaaga atcaggcacg aatatctcgt tacatctttc gattcaggct
     3601 tctatcaggt tccgccggta tatgcagaaa tgagaacaga gtcgggtata aaacgttatt
     3661 attcagacta ttcatggctt aaggttatga gggttaacat tactccaccc gatactgcat
     3721 cggcaatatt tgacatagtt gaccccggaa aagttccatt aactgctgat gagattatac
     3781 catggatagt ggctattctg gcagccggag ctctgatatg gcttctgtta tatttatgga
     3841 aacgcctcaa aaaacctgaa acaactattg ttgataataa accaaaagaa cctgcacatg
     3901 taattgcatt cagagaactt gaaaaattga aagaggaaaa actgtggcag aaaggtgaga
     3961 taaaactcta ttattcgaga cttacagaaa taataagaca ataccttttg aacaggttca
     4021 ctgtccctgc acttgaaatg actacagttg aaacactgga tgctttacgg aataaaggaa
     4081 ttgaagaaga cgaaactttc aggaaattac gaacaatact aacaggtggg gaccttgtaa
     4141 aatttgccag ataccagcct gagccaactg aaaatgagtt acattttgac tacgcatggg
     4201 attttgtgaa atcgacaatg atagtttccc ctccaccgga agaggaggtg aaagctgaaa
     4261 atgtgaagga gggtatatga ttagaggtat tgtatttgat tcgccagggt tcctttattt
     4321 gctggcacta ttaccactga tgattttcct gtacgtcata aggtacaata aatcatcagc
     4381 tgcattgcgt gtttcatcag ctgaagcttt taataattca ggcaagacct tcaggcatat
     4441 tttaaggcat gtcctcttcg gaatgagagt tgctgcaact gggttgctga ttattgtact
     4501 tgccagacct caggcaacca acagctatca ggatgtatcg act
//
LOCUS       DAOSNB010000208         4539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19165_length_4539_cov_4.028323, whole genome shotgun sequence.
ACCESSION   DAOSNB010000208 DAOSNB010000000
VERSION     DAOSNB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19165_length_4539_cov_4.028323"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLG42_08470"
     CDS             <1..483
                     /locus_tag="PLG42_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOU31280.1"
                     /translation="YRAAQGELNVFLVVLSGTAGALAGALVNYYLALWLGRPLVYRFA
                     NSAIGKMLLLSEEKIRKAETFFIKNGRSSTFIGRLVPAVRQLISIPAGLAKMDMKVFI
                     IYTVLGAGIWNIILAVIGYFLYDVREEIFPYIGHILIALGLAFLIYLIIKSVRKKNNN
                     "
     gene            complement(574..1395)
                     /locus_tag="PLG42_08475"
     CDS             complement(574..1395)
                     /locus_tag="PLG42_08475"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU31281.1"
                     /translation="MTNTGYTIDVKKAIQNSNNRLLKSLPGFAINIIRRIICEKELND
                     SLNRSSHLTGIPFIINVLEGWNVKVIAKGEENIPQQGRFIFVSNHPVGGIDAMAFFSV
                     IYRHFAEVVSPANELLNYIPNLRPLFLSLDVFGRISREAAKQLDELYASDKQVMIFPA
                     GEVSRRSKGKISDIAWQKSFITKAIQHKRDIIPVHISGRNSNLFYNIANLRKMLGIKM
                     YIETILLPREMMRQRNSTVTLTIGKVIPWQSLSAEKTHEEWAQEIKRIVYSLPDA"
     gene            complement(1429..2277)
                     /locus_tag="PLG42_08480"
     CDS             complement(1429..2277)
                     /locus_tag="PLG42_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU31282.1"
                     /translation="MRLTVLILFLGFLFLNVNAQKKEVIYLWPGKVPGEQKEKLPPVI
                     SSPGADNVIRYEEVTNPLIEVYLPEASANKGAGVVVCPGGAYRILAYNKEGTEIAEWL
                     NRLGYAAFVLQYRVPDKREGALQDAQRAIRIVRANASRWNIDPGKVGIMGFSAGGSLS
                     ARSATQFNRRTYTAVDKTDSLSCRPAFALLIYPAYLDQGPGNTLTPELTLTPETPPFF
                     IFQTADDTYGNSAIVMTGALRMAKIPVELHVLPSGGHGYGLRPGKEAAETWPLLAEKW
                     LKKITE"
     gene            complement(2298..2651)
                     /locus_tag="PLG42_08485"
     CDS             complement(2298..2651)
                     /locus_tag="PLG42_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31283.1"
                     /translation="MKTSVNFSSALIFFMLLILLFTGCARSEDVGACLSGHTYGFFGG
                     LWHGFIAPFDFIGMLFNDNITMYAQNNNGALYALGFLLGSGGWGFFGSRGARKIRYTR
                     VRTKNDMFDDAEIVK"
     gene            complement(2671..3096)
                     /locus_tag="PLG42_08490"
     CDS             complement(2671..3096)
                     /locus_tag="PLG42_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31284.1"
                     /translation="MRDILQKLNYRNQGRIAILNAEKKFLRMIRSGRPELKIDTEIDP
                     RFPYEFMIIFVRFLAEVDEIAPKAIHNLVSDGVLWFAYPNRKSSARIASDLDNAHGWE
                     ILLERGFDRVRQVIIDENWSAIRFRNIRYIKSPKYQLHS"
     regulatory      3233..3328
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3382..>4539
                     /locus_tag="PLG42_08495"
     CDS             3382..>4539
                     /locus_tag="PLG42_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOU31285.1"
                     /translation="MKARFILSVAFLVIFPLFVSGQALSVHGKVTDENGAPLPGAGIT
                     VLNTYSGTYSGEDGSYTLKLPARGRYSLRFSFTGYETAVYEIDLQGDETLDVQMKPAT
                     FLTDEVLVTATRAGYRTPVTYSVVTGEELKKLNLGHELPFMLALTPSLVETSEAGTGI
                     GYTAFRIRGTDPSRINVTVDGIPLNDAESQQVFWVDLPDLASSVENIQVQRGVGTSAN
                     GAGAFGASVNIMTKTPSNEPFAGIGISAGSFNTFRKTLEAGTGLLPGNIALQIRYSDI
                     KSDGYIRRTGCDNRSAVVTGVWKSGSSLLKANVILGEEHTGISWWGVPSDKLSTDRRY
                     NPAGEYTDDNGKTKYYDNETDNYWQNHYRLLFSTNITDNLNFNAALHYTYGK"
BASE COUNT         1248 a         1024 c          984 g         1283 t
ORIGIN      
        1 taccgtgctg cacagggtga acttaacgta tttctggtag tcctctccgg tacagcaggt
       61 gcccttgccg gagcactcgt taactattat ctggcactgt ggcttggacg acctctggtt
      121 tatcgctttg caaactcagc catcggaaaa atgcttcttc tgtcggaaga aaaaatcaga
      181 aaagccgaaa cattttttat caaaaacggc aggtcgtcaa ctttcatagg ccggctcgtt
      241 cctgcagtac gacagcttat ctccatccct gccggacttg caaaaatgga tatgaaagtc
      301 tttattatct ataccgttct cggagccgga atatggaata taatccttgc agtaatcggc
      361 tattttcttt atgatgtcag ggaagaaatc ttcccctata tcggacatat acttattgcc
      421 ctaggtcttg catttcttat atatttgata atcaaatctg taagaaagaa aaataataat
      481 tagttattca ctataaaacc tgtagcgttt cattggcaga caggaagaaa tctgaataaa
      541 gggaagagga ggggagtata aaattcaagc actctatgca tcgggaagtg aatagacaat
      601 tcttttaatt tcctgtgccc attcctcgtg ggtcttttct gccgacaggg attgccaggg
      661 tataactttc ccgatggtaa gcgtgaccgt tgaatttcgc tgacgcatca tttcacgggg
      721 gagcaggata gtctcgatgt acatttttat acctaacatc ttgcgaagat tggcaatgtt
      781 gtagaaaaga tttgaatttc taccactgat atgtaccggg attatatctc ttttatgctg
      841 tatggctttg gtaataaacg atttttgcca tgcgatatct gagatttttc ccttgctccg
      901 gcgtgatact tctcctgcag gaaatatcat aacctgttta tctgatgcat ataattcatc
      961 aagctgcttt gctgcttccc gggaaatgcg tccaaacaca tcgagactga gaaaaagggg
     1021 tcggaggttg gggatatagt taagaagctc gtttgcagga gatacgactt cagcgaaatg
     1081 cctgtatatt acactgaaga atgccatggc atcaatacct ccaacaggat ggtttgatac
     1141 aaaaatgaat ctgccctgct ggggaatgtt ttcctcgccc ttcgcaatta cttttacatt
     1201 ccagccttcc agcacattta ttataaaagg tataccagtg agatggctgc ttctgttaag
     1261 cgagtcattg agttctttct cgcagattat acgtctgatt atatttattg cgaaacctgg
     1321 aagtgacttg agcagccggt tattgctgtt ttgaatggct tttttaacgt caatggtata
     1381 tccggtgttc gtcacaggtt aaatatcagc attttatgtc actgaaacct attcggtgat
     1441 tttcttcagc catttttctg cgagcagggg ccatgtttct gcagcttctt tccccggtcg
     1501 taatccatat ccatgaccac ctgaaggcag cacatgaagc tcaacaggta tctttgccat
     1561 gcgtagtgcg ccagtcatta caatggcact gttgccgtag gtgtcgtcgg cggtctggaa
     1621 tataaagaaa ggcggtgttt cgggtgtcag ggtcagttcc ggggtgagag tgttacccgg
     1681 tccctggtcg agataagccg gatagatgag aagggcaaaa gcaggcctgc atgagagtga
     1741 atcggttttg tcaactgctg tgtatgttcg cctgttgaat tgtgtagcac ttcttgcact
     1801 caggcttccg ccagccgaga aacccattat gcccaccttc ccgggatcaa tgttccacct
     1861 tgaggcatta gctcttacaa tgcggatagc cctctgtgca tcttgaaggg caccctccct
     1921 tttgtcaggc accctgtact gcagcacaaa ggctgcatac ccgagcctgt taagccattc
     1981 cgctatctcc gtcccctcct tgttgtatgc cagtatgcgg tatgcccctc ccggacaaac
     2041 aacaacacct gcacctttgt ttgcagaagc ctctggcaga taaacctcaa taagcggatt
     2101 tgtaacttct tcgtacctta taacattgtc tgcaccaggt gaagaaatta ccggcggaag
     2161 tttttccttt tgctcacccg gtactttacc cggccataaa tatattacct ccttcttctg
     2221 ggcattaaca ttaagaaata aaaatccaag aaaaagaatc aatacagtaa gtctcatatg
     2281 aaaattattt gtaaggttta tttaactatt tcagcatcat caaacatatc attttttgta
     2341 cgaacccttg tatatcttat tttcctggca ccgcggctcc cgaagaatcc ccatccgccg
     2401 ctgccgagaa gaaaccccag agcgtaaaga gctccgttgt tgttctgagc atacatggta
     2461 atattatcat tgaaaagcat gcctataaaa tcaaacggag caatgaaccc gtgccataaa
     2521 ccgccgaaaa aaccataagt gtgacccgaa aggcatgctc ccacatcttc actcctggca
     2581 cagcctgtaa aaagcaaaat cagaagcata aaaaatataa gtgctgagga aaaatttact
     2641 gaagttttca ttggtgacaa agtgttgatt ttaactatgt aactgatatt ttggcgattt
     2701 aatgtatctg atatttctga agcgtattgc cgaccagttt tcatctatta ttacctgacg
     2761 aaccctgtcg aagcccctct caagcaatat ttcccatccg tgagcattgt cgaggtcaga
     2821 ggcaatcctg gctgagctct ttctgttcgg atatgcaaac cagagaacgc cgtccgatac
     2881 caggttatga attgcctttg gcgctatttc atcaacttca gccaggaacc tcacaaagat
     2941 aatcataaac tcataaggga agcgtggatc gatttcagta tcaattttta attcgggcct
     3001 gccgctgcgt atcatcctca gaaatttctt ttcagcattc aaaatggcaa ttcgcccctg
     3061 attcctgtag tttaattttt gcagaatatc cctcatcccc gctgtgttaa aatttttaac
     3121 ctgaaacaga tataacaatt tcccggtcaa atcaggaagc taagttagtt aaataattca
     3181 aagcaaaaat tgaatggttt aaaagaaaat aaaaaatatt aatatctttg ttctgtcttt
     3241 aatggggtgc cttaatacta cgggctgaga tcataccctt gaacctgatc cgggtaatgc
     3301 cggcgtaggg aaaaaagagt atcacatatc cagcgctgtt tcacctcatt ggttaaaaaa
     3361 acaatttttg actgatgaat tatgaaagcg agatttattt tatcagtggc cttcctggtc
     3421 atttttccgt tatttgtgtc aggtcaggca ttatctgttc acggtaaggt gacagatgaa
     3481 aacggagcac cattgcccgg tgccgggata acagttctta atacttattc aggaacttat
     3541 tccggcgaag acgggagtta cactcttaaa ctgcctgcca gaggcaggta ttctctgagg
     3601 ttttcattta caggatatga gactgcagtg tatgagattg acctgcaggg ggatgaaaca
     3661 ctggatgtgc agatgaaacc tgccaccttt ctcaccgatg aggtgctggt aactgccaca
     3721 agggcaggct accgcactcc ggtaacttat tcggttgtga cgggtgagga gttgaaaaag
     3781 ctcaatctgg ggcatgaact tccgttcatg cttgccctta caccatcgct tgttgagaca
     3841 tccgaagccg gaacaggcat tggctatacg gctttcagaa taagaggtac cgatcctagc
     3901 cgcatcaacg taactgtcga cggaatacct cttaatgatg ccgagtcgca gcaggtcttc
     3961 tgggtcgacc tgcccgacct tgcctcttcg gtagaaaata ttcaggtgca gcgcggtgtt
     4021 gggacttcag ccaacggtgc cggagctttc ggtgcttctg taaatataat gacgaaaact
     4081 ccttcaaatg aaccgtttgc tggaatagga atctctgcag gctctttcaa tacatttcgc
     4141 aagactcttg aagccggaac gggccttcta ccgggcaata ttgcccttca gataagatac
     4201 tccgacatca aaagcgacgg ctacatacgc cgcacaggat gcgataaccg ttctgctgtc
     4261 gttactggag tctggaaatc aggcagctca ctgctaaagg caaatgtcat actcggtgaa
     4321 gaacacaccg gcataagctg gtggggagtg ccttccgaca aactgagcac cgaccgcaga
     4381 tataaccccg caggagaata taccgacgac aacggcaaaa cgaagtacta tgataacgaa
     4441 actgataact actggcagaa tcattacagg ttactcttca gtacaaacat tactgataac
     4501 ctgaatttta atgctgccct gcattataca tacggcaaa
//
LOCUS       DAOSNB010000209         4530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19216_length_4530_cov_3.655196, whole genome shotgun sequence.
ACCESSION   DAOSNB010000209 DAOSNB010000000
VERSION     DAOSNB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19216_length_4530_cov_3.655196"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PLG42_08500"
     CDS             <1..1051
                     /locus_tag="PLG42_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31286.1"
                     /translation="PDWPVAADLSLNESIELGWPLEVALFFTGDIKQQASTFAEWLES
                     RNPDIARILLFHRDKPATPDDMAKEVITILRKKNPDIRIATGTNANFAQLNRNRPGDT
                     GNDYITYSIHPQEHASDDTTLIENLKGQEYSVRSAKNFAGRKGVVVSPVTLKKRFNPG
                     ISFIELPWEGEGVPPQIDLRQMTLFGACWTAGSLKYLCEADVDSITYFEAVGERGIMQ
                     GEKEPEWPEFFPSTKGMIFPVFHLFRYVLLHNDYNILKSISSDPLCADCFSMASDGEA
                     RLVMVNYTDSEQNVKLEGLSGMFRMRSLNQTTLKEAVSDCRWTGIKDEKIYNSEKIIT
                     LQPYSLNFIEGWLKG"
     gene            complement(1079..1843)
                     /locus_tag="PLG42_08505"
     CDS             complement(1079..1843)
                     /locus_tag="PLG42_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU31287.1"
                     /translation="MKSNLLKINILLIIIMIASCTEKPVTDPMTLYSEVDLNAAPNAL
                     TKKEAKKGWQLLFDGKTTSGWRGYNMKEFPDCWAIEDGALTMNTTGGAESQDIITTRK
                     YKNFVLSLEYKLTPAANSGIMYQVEEDTIYKFPYETGPEFQVIDSDGWPEKLEEWQVN
                     GANYAMYPPRVKPYKRVGEWNHAMLIVNGNKVTQILNGEIVVEYEKYSDEWNKLRNSG
                     KWNDYPDWGKFDEGYIALQNHGTKVWYRNIKLKELD"
     gene            2031..3452
                     /locus_tag="PLG42_08510"
     CDS             2031..3452
                     /locus_tag="PLG42_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOU31288.1"
                     /translation="MKKSFILFAVALFFAVTGIVTVSGQVKLPSIFGDNMVMQQQTDA
                     AIWGNASPNSNVTIKTSWNGRSYTAKAGPDGRWKTRVSTPAAGGPYTITISDGKPLVI
                     KNVMIGEVWVCSGQSNMEMPMKGYRNQPVLGANEYIATSSNNSIRLITVPHATSLTPL
                     DDFKGQWKLCEPENVAEFSATAYFFGLMLNKALNVPVGLICTSWGGTRIEPWMSEEGL
                     KPFEWVKLPDKTAKVEKLSQQTPTVLFNAMVNPIVGYGIRGAIWYQGEANRKEPKEYQ
                     KLMPGLALDWRRVWGIGEFPMYYVQIAPYDYGEQGFNSAFLREAQLKAATAIPNFGMA
                     CVMDTGEKDCIHPANKKAAGDRLAYLALAKTYGKKGFACEGPVLKEMRVEGSLVYLTF
                     DNAPNGLTSFGKELSCFEVAGANRRFFPAKAFITNAGITLFSPLVPQPVAVRYAFKDF
                     IVGDLFNTEGLPASSFRTDDWEQ"
     gene            3511..>4530
                     /locus_tag="PLG42_08515"
     CDS             3511..>4530
                     /locus_tag="PLG42_08515"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HOU31289.1"
                     /translation="MNEAKQITFHLIFLVWSIALVAQPPLQYVNPLIGTAPATTESAR
                     RHGSGTEDKGQTFPAVGRPFGMTQWTPETRTTETKCMAPYYYNDKYLTGFRGSHWMSG
                     SCTQEYGSATIMPFTSSKPDTITVNPVSTLNHKSETVSPAYYRINLSTFGITAEITGT
                     TRAGMMRFTFPGKPEGFILIRMNSDEKEGKVWIDREKNEIIGYNPVHRIYQGWGKPAG
                     FSGWFVIKTDKPFQEVSYLKNEQQLVISVGAEKTILVKAGTSFTGAEAARNNMIKEIP
                     GWDFDAVRRESENVWNQTLSKIIVRNGPDDDKIKFYTALYHCYLLPRIASDADGSYIC
                     FSSGGK"
BASE COUNT         1370 a         1015 c          968 g         1177 t
ORIGIN      
        1 acccgactgg cctgtggcag ccgacctttc gctgaatgaa tcaattgagc ttggatggcc
       61 actggaagtt gcattgtttt ttacaggcga tataaaacaa caggctagta cgtttgcaga
      121 gtggcttgaa tcgagaaatc ctgatattgc aagaatatta ctgtttcacc gtgataagcc
      181 tgcgacacct gatgatatgg caaaggaagt aataaccatt ttgcgtaaaa agaaccctga
      241 catcagaatt gccacaggta ccaatgcaaa ctttgcccag ctaaaccgca ataggccggg
      301 tgacaccgga aacgactata tcacatattc aattcatccc caggaacatg cttcagacga
      361 taccactctt attgaaaatc taaaaggcca ggaatatagt gtcagaagtg caaaaaattt
      421 tgcaggcaga aaaggtgtgg tggtgtcacc agttacttta aaaaaaagat ttaatcctgg
      481 aatttcattt atcgaacttc catgggaagg agaaggtgtt ccaccacaga ttgacctcag
      541 gcagatgacg ctatttggag cctgctggac tgccgggagc cttaaatatc tgtgtgaagc
      601 agatgttgac agcatcacat attttgaagc cgttggagaa agaggaatca tgcagggaga
      661 gaaagaacct gaatggccgg agtttttccc ctcaacaaaa ggaatgattt ttccagtatt
      721 tcatctcttc cgctacgttc tactgcacaa cgattacaac atattgaaaa gtataagttc
      781 tgaccctctc tgtgccgact gtttttcaat ggcaagtgat ggtgaagcaa gactggtaat
      841 ggtaaattat accgattcag aacagaacgt taaacttgaa ggattatcgg ggatgttccg
      901 catgagatct cttaatcaga caactttaaa agaagctgtc agcgactgcc gctggacagg
      961 gataaaagat gaaaaaattt acaattctga aaaaataata acccttcaac cgtactccct
     1021 aaattttatt gaaggatggc tgaagggctg ataggatgac gtcaggttat tttacacctc
     1081 aatcaagttc cttgagcttg atatttcgat accacacctt cgttccatga ttctgtaacg
     1141 caatatagcc ctcatcgaat ttcccccaat cgggataatc attccattta ccactgttac
     1201 gaagcttgtt ccattcatca gagtactttt cgtactctac aacaatttct ccattcagta
     1261 tctgagtcac tttattacca tttactatca gcattgcatg attccattct ccaacacgct
     1321 tatatggttt tacacgggga ggatacatgg cgtaattggc gccgtttacc tgccactcct
     1381 caagcttttc tggccagccg tcactgtcaa ttacctgaaa ttcaggaccg gtctcataag
     1441 gaaatttata aatagtatcc tcctctacct gatacattat cccactgttt gcagccggag
     1501 taagtttgta ttccagactc aacacaaaat ttttatactt gcgcgttgtt ataatatcct
     1561 gactttcggc acctcctgtt gtgttcattg taagtgcccc gtcctcaatt gcccagcaat
     1621 ccgggaactc tttcatatta tatccgcgcc agccactggt tgtttttccg tcaaacaaca
     1681 attgccatcc tttctttgcc tctttctttg ttaaagcatt gggagcagca ttcaggtcaa
     1741 cttcactgta aagcgtcata ggatccgtta caggcttctc ggtgcaggaa gcaatcataa
     1801 taataatcaa taaaatgtta attttcaata aattgctttt cataatttta aaatttgaat
     1861 tatacatttt tattcattca gatattttca taaaattact tcaaattttt ctttcattgc
     1921 aaaaaagttt ttatgttaaa ctgtaagcaa aaaaatctgc taaattcaca tgcgatttac
     1981 atctgagaaa catacaataa acaattaacg acatttaata atttccagcc atgaaaaaat
     2041 catttattct attcgctgtt gccctgtttt ttgctgtaac cggtatagtt accgttagcg
     2101 gacaggtcaa gcttccttcc atctttggag acaatatggt tatgcagcaa cagactgatg
     2161 ctgcgatatg gggaaatgca agtcccaact caaacgttac gattaaaact tcgtggaatg
     2221 gcaggagtta taccgccaaa gccggacccg acggcaggtg gaaaaccaga gtgtccaccc
     2281 cggcagcagg aggaccttat acaataacaa taagcgatgg caaaccactg gttatcaaaa
     2341 atgttatgat aggagaagta tgggtttgct ccggacaatc gaacatggag atgccgatga
     2401 agggttacag aaaccagccg gtgcttggtg caaacgagta tattgcaact tcatccaata
     2461 actctatcag gcttattaca gtcccccatg caacgagcct gacgccactt gatgatttca
     2521 agggccagtg gaaactatgt gaacctgaaa atgttgctga attcagcgct actgcttact
     2581 tcttcggatt gatgctcaac aaagcgctga acgtacctgt cggacttatc tgtacaagct
     2641 ggggcggtac gcgcattgag ccatggatga gcgaggaagg actcaaacca ttcgaatggg
     2701 taaaactgcc tgacaaaaca gcaaaagttg aaaaactctc ccagcaaaca ccaacggtac
     2761 tctttaatgc aatggttaat cccattgtgg gttacggcat ccgtggtgca atatggtatc
     2821 agggtgaggc aaaccgcaag gagcctaaag agtaccagaa actgatgccc ggtctggctc
     2881 tcgactggag gagggtatgg ggaataggag aattcccgat gtactatgtc cagattgcac
     2941 catatgatta tggggaacag ggattcaatt cagcttttct tcgtgaggca cagcttaaag
     3001 ctgccactgc aataccaaat ttcggaatgg catgcgtgat ggacaccggt gaaaaggatt
     3061 gtatacatcc tgcaaacaaa aaggcggccg gcgacagact ggcatatctc gctctggcaa
     3121 agacatacgg caagaaagga tttgcctgcg aaggccctgt gttgaaagag atgagggttg
     3181 aaggcagtct ggtttacctt actttcgaca atgctccaaa cggactgact tctttcggga
     3241 aagaattgtc atgttttgag gtggcaggag caaacagaag gtttttccct gcaaaagcat
     3301 ttataaccaa cgccggaatt accctgtttt cacccctggt gccccagccc gttgccgtca
     3361 gatatgcctt caaggatttt attgtaggtg atctgttcaa taccgagggt ctgccggcat
     3421 cctcattcag aactgatgac tgggaacaat aagaaagtca ttcaatagta attctgcagt
     3481 aattcaatag taattcagtt gaaattaaca atgaatgaag cgaagcaaat aacattccat
     3541 cttattttcc ttgtttggtc tatagctctg gttgcacagc ctcccctgca gtatgtcaat
     3601 cccctcatcg gcacagcccc tgccacaacc gaaagtgcgc gtcgtcatgg ctcaggaaca
     3661 gaagacaaag gtcagacctt ccccgctgtg ggacgaccct tcggcatgac acaatggaca
     3721 cccgaaacac gaaccaccga aacgaaatgc atggctccat actactataa cgataaatat
     3781 ttaacaggct tccgtggaag ccactggatg agcggcagct gtacacagga atatggctct
     3841 gccaccataa tgcccttcac ttcatcaaaa ccagatacca taactgtaaa ccctgtttca
     3901 acattaaatc ataaatccga aacagtctcc ccggcttatt accggataaa cctcagcaca
     3961 ttcggaataa ctgctgaaat aaccggcacc acaagagcag gaatgatgcg ttttaccttc
     4021 ccgggcaaac cggaaggatt tatactcata cggatgaaca gcgacgagaa agaaggcaag
     4081 gtgtggatag acagggaaaa aaatgagata ataggctata acccggttca cagaatctat
     4141 cagggatggg ggaaacctgc aggtttcagc ggctggttcg tcatcaaaac cgacaagcct
     4201 tttcaggaag tctcatacct gaaaaatgaa cagcagcttg taattagtgt cggtgctgaa
     4261 aagacaatac tggttaaggc aggcacatct tttacaggcg ctgaagccgc ccgtaacaac
     4321 atgataaaag agataccggg ctgggacttc gacgcagtgc gcagggaaag cgaaaacgtc
     4381 tggaaccaga cactaagtaa aataatagta agaaacgggc ccgacgacga caaaataaaa
     4441 ttctataccg cattgtacca ttgttacctc ctcccgcgca tcgccagcga cgctgacggc
     4501 agttatatct gcttttcttc gggaggtaaa
//
LOCUS       DAOSNB010000210         4497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19381_length_4497_cov_4.494147, whole genome shotgun sequence.
ACCESSION   DAOSNB010000210 DAOSNB010000000
VERSION     DAOSNB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19381_length_4497_cov_4.494147"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLG42_08520"
     CDS             complement(<1..783)
                     /locus_tag="PLG42_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31290.1"
                     /translation="MRKNYSFVLLFIILFITTCRKDNDKFWYSSSVSFKITRHLLEGR
                     KINCLATSNDGTLFVGSENDLYIIRGNNQEKFDLGSPVMDIAVDKSSVLWIGTGGKGL
                     CRLENRKFTWYNKENSGFPRDYIAEVEIDKNGNIWFSSCAHMLGGIGIFNGNKFEFLT
                     PENSPLNQNVISDMVAGEDGAVYIATSGKVGKSNIYRIYNKEWDCLGNENGTFYWIFS
                     FTVSPAGIIYVIEDFSLSSAFETNKVYSFENGNWHLLNKNENP"
     gene            complement(1066..2328)
                     /locus_tag="PLG42_08525"
     CDS             complement(1066..2328)
                     /locus_tag="PLG42_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOU31291.1"
                     /translation="MLSSSKREEVVFYFIDMNSFGWQKVYEYSKGAGGCRNDIIPDNE
                     IHTLFRLIENAGGVPYRIIMENDVAGSENCLIIVGNGIRDLLGISPEDFKEGIYRSIV
                     EKIVSLDNNIPVEIIEARKELFSGKIENFRVELQVKMPGGGKKRIFEFATPVVDSETG
                     KVKEICGIFFDPEKWNSFNVELTKKYDNHEFENFKKTFLRNISHEIRTPLNAIIGFSS
                     ILCEPECPQEKREEYAEIILKSADTLLETVNDIIEASQIESGTVKIKKEPANLNSIIR
                     SVYDRLKKKAGDKGINISYRVPLDDEDVNIMTDQEKVKRVLQNMGDNAVKFTDGGEVE
                     IGYVISGGMVEFYVYDTGKGISEEYYNYIFTSFFQADNGPSRLHGGTGLGLYISKGYV
                     ELLGGKIWFFSEPGEGTTFFFSIPFERV"
     gene            2511..3104
                     /locus_tag="PLG42_08530"
     CDS             2511..3104
                     /locus_tag="PLG42_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU31292.1"
                     /translation="MKEYSDSEIIECLRNRQGYVVSYLMERYLPMIRLMVVQFGGTNE
                     DARDIFQEGLMIMLEKIDDRNFVLTCKFKTLFYCVCENLWKMVLAKRKSAENFFHRKT
                     EEESEKDPGEKIDDELYQRIFRESFDSLDELSKEILKLYWQEKSPAEIAEILGYTYGY
                     VKKKKSEAQAELTKKLKQHPDYRKIMKLEKLTGTPVV"
     gene            3152..4180
                     /locus_tag="PLG42_08535"
     CDS             3152..4180
                     /locus_tag="PLG42_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31293.1"
                     /translation="MRLRTEHNRINDNLAKSGNNGQSWFDEDSVLSSHDQALLRLTGE
                     YLTAMSDIEEVKNDPDYLVASSLAVEMIREYKEKPVWSRDNEKFIRESFEKAEEEKSI
                     IQEISEIKAEAEKKGVFETAEEWVNDWNRRKVAGRTETDKIREIENFVTGSLNNEISE
                     PEVRYNNRSKKPVVKILRYVAIPAAAAIGMFIMLRIFTAPFNTEKIYSEIYQPLPAIT
                     GITRGGAATIRDNFSEGIVKYRKGDYYGAALSFSEAMKTDSSSFAVKFYQGITQIALG
                     NYSSAVNLLENITGSETEYGKDACWYLGLAYLKLGEKEKALKYLTPLSESRGFYRDKA
                     RKIVRRLR"
     gene            complement(4138..>4497)
                     /locus_tag="PLG42_08540"
     CDS             complement(4138..>4497)
                     /locus_tag="PLG42_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOU31294.1"
                     /translation="VISLARSFLLAGASSVVRTKWDINDETSAKIMSSFYKYLSKGKT
                     KDKALRQAKLDYLATMPPVYSNPYYWGAYELLGDNSSLATNKRIYLLVTGSIIILCIP
                     VFVIYLRRRTIFRALSR"
BASE COUNT         1448 a          832 c          831 g         1386 t
ORIGIN      
        1 aggattttcg tttttgttca gtaaatgcca gttaccattt tcaaaggaat atactttatt
       61 tgtttcaaaa gccgaggata gtgaaaaatc ttcaataaca tatattatac ctgccggact
      121 tactgtaaat gagaaaatcc agtaaaatgt accgttttca ttgccgagac aatcccattc
      181 cttgttgtaa attctgtata tgtttgattt gccaaccttt cctgaagttg caatatatac
      241 agctccatct tctccggcaa ccatatctga gataacattc tggttcaggg gtgagttttc
      301 gggggtaagg aattcgaact tgtttccgtt gaaaatgcca atgcctccaa gcatatgtgc
      361 acaactggag aaccagatat taccattctt atcaatttca acctcagcaa tatagtcacg
      421 cgggaatccg gaattttctt tgttgtacca ggtaaatttc ctgttttcta atctgcataa
      481 accttttcct ccagtgccaa tccacaacac tgaactctta tccactgcta tatccattac
      541 tgggcttccg aggtcaaatt tttcctggtt atttcccctg ataatataaa gatcattttc
      601 agaaccaacg aaaagagttc cgtcgttaga agttgccaga cagttgattt tcctgccctc
      661 aagcagatgg cgtgtaattt tgaagctaac cgatgatgaa taccaaaatt tatcgttatc
      721 ttttctacag gtagttatga acaatataat gaataatagc acaaatgaat aattctttct
      781 catgaataag aggttaatgc attaaaagcc atataaaaat aacgccaaag tattgcaaaa
      841 attataccat taatcagagt atttaagaaa taaggtagca taatgaaaat ttcaattatg
      901 gatcacctat tttagttagt atgggatgag ttatgtgaag agcatttaac tttccaattt
      961 taaaaaagga acagggacta tcagtcctgc agacatagac attgacgaaa tacccttgta
     1021 agaacctggc tttttgccgg attatatttt taactatttc tttttttaaa ctctttcaaa
     1081 aggaatgctg aagaaaaacg tagttccctc accaggttca gaaaagaacc agatcttacc
     1141 cccaagaagt tcaacataac cttttgagat gtagagacca agaccagtac ctccatgcag
     1201 gcgcgacggc ccattgtcgg cctgaaagaa actggtgaaa atataattgt aatactcttc
     1261 tgatatacct ttgcccgtgt cgtatacata gaattctacc atgccacccg atattacata
     1321 gccaatttcc acttctcccc catctgtaaa cttcactgca ttatctccca tattctgaag
     1381 aacccgcttt accttttcct ggtctgtcat tatattcaca tcttcatcat ccagaggaac
     1441 tctatatgaa atatttatac ccttatcacc agcttttttc ttcagtctgt cataaacact
     1501 tctgataatt gaattcagat tagcaggctc cttttttatc ttcacggttc ctgattcaat
     1561 ttgtgatgct tctatgatat cattaactgt ttcaagcaga gtatcggcac tctttaatat
     1621 aatttctgca tattcttccc tcttttcttg agggcattcg ggttcacata aaatggagct
     1681 aaatccaatt attgcattta acggagtcct tatttcgtga gatatatttc tcaggaaagt
     1741 ttttttgaaa ttttcaaatt catgattatc atattttttg gttaattcaa cattaaatga
     1801 attccacttt tcaggatcaa aaaatatccc gcatatctct tttactttgc ctgtttctga
     1861 atcgacgact ggtgttgcaa attcaaaaat tctttttttc ccgcctccag gcatttttac
     1921 ctgcaattca accctgaaat tttctatttt gcccgaaaac agttcttttc tggcttcaat
     1981 tatttctacg ggaatattat tgtcgaggga aactattttt tcaacgatgc ttctgtaaat
     2041 cccttctttg aaatcttccg gtgaaatgcc cagcaggtcc ctgattccat ttccgactat
     2101 aattaagcaa ttttcagacc cggcaacatc attttccatt attatcctgt aaggaacccc
     2161 tcctgcattt tcaattagcc tgaaaagtgt atgaatttcg ttatcaggaa taatatcgtt
     2221 cctgcatcct ccagctcctt tagagtattc gtaaaccttc tgccatccaa atgaattcat
     2281 atcaataaaa taaaatacta cttcttccct tttcgaagaa gaaagcataa taaagtatca
     2341 tctgttttat caatatacct tatagtcttt ttcagttttc actgtttaat tgcgattgac
     2401 ccaaaattgt tacctgtttt ttaaaaaaat ttttatatct gttgataact ttttatttat
     2461 ttataaattt gtcagtgaat tacgggtgtg cgaataaaga gttttaacag gtgaaagaat
     2521 attctgacag cgaaataatt gagtgtctgc gcaaccggca gggttatgtt gtcagttacc
     2581 tgatggaaag gtacctgcca atgataaggc ttatggttgt gcaatttggt ggcacaaatg
     2641 aagatgcgcg cgacattttc caggaaggcc tgatgataat gcttgagaag attgacgaca
     2701 gaaactttgt tctgacctgc aagttcaaaa ccctttttta ttgtgtctgt gaaaacctct
     2761 ggaaaatggt tctggcaaaa agaaaatcag ccgaaaactt tttccacagg aaaactgagg
     2821 aagaatcaga gaaagatccc ggggaaaaga tagacgatga actataccag aggatattca
     2881 gggaatcatt cgattcgctc gatgaattga gcaaagagat acttaaatta tactggcagg
     2941 aaaaatcacc tgccgaaata gcagaaattc ttggctatac atatggatat gtaaagaaaa
     3001 agaaaagtga ggcacaggct gaactgacaa agaaattgaa acagcaccct gattacagaa
     3061 aaataatgaa gctcgaaaaa ctgacaggta ctcctgttgt ttaaataatc aaacattgac
     3121 ttctgattaa ggttaaagac aaaaaatgaa tatgagactg agaacagaac acaacagaat
     3181 aaatgataac ctggcaaaat ccggtaataa cggacagtca tggtttgacg aagattctgt
     3241 tctcagttct catgatcagg cacttttaag gctgacaggt gaatatctga ctgccatgtc
     3301 tgatatcgaa gaagtgaaaa atgacccgga ttatttagta gcgagttctc tggcggttga
     3361 gatgattcgt gaatataagg agaagccagt atggagccgt gacaatgaga aatttataag
     3421 ggaatcgttt gaaaaagcag aggaagaaaa aagtataatt caggaaataa gcgaaattaa
     3481 agctgaagct gaaaagaaag gtgtttttga aactgcggaa gaatgggtca atgactggaa
     3541 cagaagaaaa gttgcaggta gaacagaaac ggataagatt agagaaatag agaattttgt
     3601 aacaggttcg ttgaataatg aaataagtga accagaagtt agatataata acagaagtaa
     3661 aaaaccggta gttaaaatat taagatatgt tgctattcct gcagcagcag caataggtat
     3721 gtttattatg ctcagaattt tcacggcacc ttttaatact gaaaaaatat actctgaaat
     3781 ataccagcca ttgcctgcaa ttacaggtat cacaaggggc ggtgctgcaa ctatcaggga
     3841 taatttcagc gagggcatag taaagtacag gaaaggagac tattacggtg cagcgctatc
     3901 gttttccgaa gcaatgaaaa ccgatagtag ttcatttgca gtaaaatttt accagggcat
     3961 tacacagatt gcacttggta attattcttc tgctgttaat ttacttgaaa atatcactgg
     4021 cagcgagaca gaatacggga aggatgcctg ctggtatctg ggactggctt atcttaaact
     4081 tggagaaaaa gaaaaagctt tgaaatatct cacgccattg tctgaatccc ggggttttta
     4141 tcgggataaa gcccgaaaaa tagtacgtcg cctcaggtaa ataacaaaaa ccggaataca
     4201 taatataatt atgctacctg taaccagcag gtagattcgt ttatttgttg caagagaact
     4261 gttgtctccc agcagttcgt atgctcccca gtaataggga ttggaatata ccggtggcat
     4321 ggttgccagg taatcaagtt tggcttgcct gagtgccttg tcttttgttt tgccctttga
     4381 aagatactta taaaacgatg acattatttt agcgcttgtt tcatcattaa tatcccattt
     4441 tgttcttaca acagaagatg caccggcaag gagaaaactg cgggcaaggc ttatcac
//
LOCUS       DAOSNB010000211         4489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19441_length_4489_cov_2.935949, whole genome shotgun sequence.
ACCESSION   DAOSNB010000211 DAOSNB010000000
VERSION     DAOSNB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19441_length_4489_cov_2.935949"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PLG42_08545"
     CDS             <1..284
                     /locus_tag="PLG42_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU31295.1"
                     /translation="LYGKQGNVENVHLANEKHDYGYSKRIVMYEFMAKHLGLDINTVK
                     DRTGKIDESKVTIETYDKLLVFGKNGKMPGNALKNPDDVWKTLLSLQKK"
     gene            312..>1429
                     /locus_tag="PLG42_08550"
     CDS             312..>1429
                     /locus_tag="PLG42_08550"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOU31296.1"
                     /translation="MIFFRNIIILLVILSTKGLLFTATSQNPRSEGYMGIWYSTGERT
                     QYGYKLSGGFATFGSRHLPTAIYSPEAQKTFFVYGGTKTPDQSHLLIMVSYFDHITRK
                     VPKPVIVYDKEGVRNPFDNASLSIDNNGYIWIFVSGWLRTRPGLVFKSAMPYSTDRFE
                     RVMIKEMSFPQPWWTTEKGFLLTFTKMREGLEINFCRSSDGYNWSEDKTITSMGGNGH
                     VSAFYNNKLYVAFNYHPGGDPDKRTNLYLIYTDDYGDTWKTIDGNEVAVPLKNVNNNA
                     LIRDFQKEGKLTYIYDINFDLKGDPVLLICTGGAPQPGPSEIPREWVVVKRENNRWAF
                     YRICETDNNFDAAALYTIGKKWIVVGPSEPGPQKYAAGG"
     assembly_gap    1430..1529
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1530..1846
                     /locus_tag="PLG42_08555"
     CDS             <1530..1846
                     /locus_tag="PLG42_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31297.1"
                     /translation="WIVVGPSEPGPQKYAAGGEMALWESTDEGATWHKAGLITSGSKF
                     NNSYARRPLNANNEFYTWWADGNAYEFSESRIYFTNSRFNRVYMLPFRMSRDYERPVK
                     VR"
     gene            complement(1850..3229)
                     /locus_tag="PLG42_08560"
     CDS             complement(1850..3229)
                     /locus_tag="PLG42_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU31298.1"
                     /translation="MNTKVLSAAPLMKKLILYIFLICFCITSNAQSYNSLNRSEETTI
                     RIKEYIDTLRIIDTHEHLLNPDFLRKTNILDFFLLMNHYYYDDLLTAGMPEEFFNKLY
                     NGNMPPEEKWKLIEPYWNRSLNTSFALVAKVAARNLFNVNEINANTVGILTQRIKSAY
                     NNKNWFETILKDSCRIEYLVQDDDALALKVDWIKYVKRFTPWLNVRTKFGIDSIAMEQ
                     VEPIFTLEDFVKSLRNEFEKGLKNGMVGIKVISAYSRNLYFEKTDIAAARKVFKTLVS
                     GDEDLQIPWEKAKPLQDYMFHQLMEMAKQHGVPVIFHTGLQAGQGNDIRNSNPVLLAN
                     LFHEYPDVRFVLFHGSYPYGGELATLAKTYSNVYIDMNWTYAISPSYAKRYLSEWLET
                     IPASKIMAFGGDYNCVENIYGVYQIAKQIITEVLTEKVSNNQLSENEARQVAKMILHD
                     NAVEFYGLP"
     gene            complement(3337..3825)
                     /gene="pelA"
                     /locus_tag="PLG42_08565"
     CDS             complement(3337..3825)
                     /gene="pelA"
                     /locus_tag="PLG42_08565"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOU31299.1"
                     /translation="MLKTQIIVNGKPTVWCAQHDENTLKPAGARSYELPSFSGAESAG
                     IVLLLMEIPTPSKKIIRAIEGAVEWLDKHRIKNVRWEYFTNAEGLRDRRIVNDPQAGD
                     MWARFYDLETAEPFFCDRDGIKKKSLDEIGYERRNGYSWYTDAPLEVFKKYSLWKEKF
                     RN"
     gene            complement(3819..>4489)
                     /locus_tag="PLG42_08570"
     CDS             complement(3819..>4489)
                     /locus_tag="PLG42_08570"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOU31300.1"
                     /translation="NKQMETSSLKRLYYPLLIKTGSKASFDMIAETYKQSTGDDKEAA
                     FKALITSSDFETIYLLLDILEANPPVANAKEAVNAILKLIASSGKTGEVQTVFLRTVM
                     PFAKDIQQQKNIIGMLGKAGTFQGLHVVATYLEKPELKEAFNKGLLFILDSQYKNGGW
                     PMFWPLRKGYYSHITFNDNATVNILRLLKDINENKQNLQNILQTISSFQKPGKHSTKA
                     LTAY"
BASE COUNT         1292 a          848 c          901 g         1348 t
ORIGIN      
        1 ctctctacgg aaaacagggt aacgtcgaaa atgtccatct ggcaaatgaa aaacacgact
       61 acggatattc aaaacgaatc gtcatgtatg aatttatggc aaaacacctt ggacttgaca
      121 tcaatacagt aaaggacagg acaggaaaaa tagacgagtc gaaggttact atcgaaactt
      181 atgataaact actggtcttc ggaaaaaacg gaaaaatgcc cggaaatgcc ctgaaaaatc
      241 ctgatgatgt ctggaaaaca ttattatctt tacagaagaa gtaacagatg ttaaactaac
      301 cggagcactc catgatattt ttccggaata ttattattct tctggttatt ttatctacga
      361 aaggtttact ttttactgct acgtcacaga atcccaggtc ggaaggatac atgggcatct
      421 ggtacagtac gggagagagg acgcaatacg ggtataaact ttcgggtggt tttgcaacat
      481 tcggatcgcg tcatttgcca actgcaattt attctcctga agcacaaaag actttctttg
      541 tttacggggg tacaaagact ccagaccaga gccatcttct gattatggtt tcttattttg
      601 accacattac aagaaaagtc ccgaaacctg taattgttta cgataaggaa ggagtgagaa
      661 atcctttcga taatgcctca ttatcaattg ataataatgg atacatatgg attttcgtaa
      721 gcggttggtt gaggacgcgg cctggattgg tttttaagag tgccatgcct tattcaaccg
      781 acaggtttga aagggttatg ataaaggaga tgtccttccc tcagccatgg tggacaacag
      841 agaaaggatt tctgcttact ttcactaaaa tgagagaggg actcgaaatt aatttctgca
      901 gaagcagcga cggatacaac tggagtgaag ataaaacaat tacttctatg ggcggcaacg
      961 gacatgtgtc ggctttctat aataataagc tatatgtggc attcaattat catcccggag
     1021 gagatccgga caaaagaacc aatttatacc tgatctatac agatgattac ggtgacacat
     1081 ggaaaacaat tgacggtaat gaggttgctg ttccccttaa aaatgtgaat aataatgcat
     1141 taatcaggga ttttcagaaa gaaggtaaac taacttatat atacgacata aattttgacc
     1201 ttaaaggtga tcctgttctt ctaatctgca cagggggagc gcctcaaccc gggccatcag
     1261 aaataccgag ggagtgggtt gttgtaaagc gcgagaacaa ccgctgggca ttttatagaa
     1321 tatgtgaaac agacaataac ttcgatgcag ctgctttata tacaattgga aaaaaatgga
     1381 ttgtcgtagg cccatctgaa ccaggccctc aaaaatatgc tgcaggaggn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atggattgtc gtaggcccat ctgaaccagg
     1561 ccctcaaaaa tatgctgcag gaggggaaat ggctctgtgg gaaagtactg atgaaggagc
     1621 cacatggcat aaggcagggt taataacatc aggaagtaaa ttcaataatt catacgcaag
     1681 gagaccattg aatgcaaata atgagtttta tacctggtgg gccgatggaa atgcctatga
     1741 attttctgaa tcgagaattt atttcacaaa cagtagattt aacagggtat atatgctccc
     1801 cttcagaatg agcagggact atgagagacc ggtaaaagtt cgctgagaat caggggaggc
     1861 cgtaaaattc cacagcattg tcgtgaagta tcatttttgc aacctgtctg gcttcatttt
     1921 ctgataattg attgttagaa actttctcgg taagtacctc tgttataatt tgtttggcaa
     1981 tctggtacac accatatata ttttccacac agttataatc tcctccgaaa gccataattt
     2041 tacttgcagg gatagtttcg agccattcgc tcagatatct ttttgcgtag gagggtgaga
     2101 tggcataagt ccagttcata tcaatataaa cgttgctgta ggtttttgcc agggttgcaa
     2161 gttctccgcc ataaggataa cttccatgaa acaaaacaaa tctgacatca ggatattcat
     2221 ggaacaggtt ggccagcaga acaggatttg aatttcttat atcattaccc tgccctgcct
     2281 gaagtccggt atgaaaaata acaggtacac catgctgctt tgccatctcc atcagctggt
     2341 gaaacatgta atcctgcaaa ggctttgctt tttcccatgg aatctgcaga tcttcatcgc
     2401 ctgatacaag tgttttaaaa acttttcttg cagctgcaat atctgtcttt tcgaaataga
     2461 ggtttctact gtatgctgat attaccttaa tcccaaccat gccgtttttt aagccttttt
     2521 caaactcatt tctgagggac tttacgaaat cttccagagt gaaaattggc tcaacctgct
     2581 ccatagcaat ggaatcaatg ccgaattttg ttctgacatt gagccaggga gtgaatcttt
     2641 taacatattt tatccaatcc actttcagcg caagggcatc atcatcctga acaaggtatt
     2701 caattctgca tgagtctttt aaaattgttt caaaccagtt tttgttgttg taagctgatt
     2761 ttatccgttg tgtcagtatc ccgacagtat tggcatttat ttcattgaca ttaaatagat
     2821 tacgggctgc aacttttgcc accagagcga atgacgtatt tagcgacctg ttccagtatg
     2881 gttcaataag cttccatttt tcttcaggtg gcatattccc gttatagagt ttattaaaga
     2941 attcttctgg catccctgca gtaagcaggt catcataata ataatgattc atcaaaagga
     3001 aaaaatcaag tatgttggtc ttcctcagga agtcggggtt gagcagatgc tcatgagtgt
     3061 caataattct taatgtatca atgtattctt taattcttat tgtagtttct tctgacctgt
     3121 ttaaagagtt atacgattgt gcattactcg taatgcagaa acagataaga aagatatata
     3181 gtattaactt tttcattagc ggagctgcgg atagaacttt ggtgttcatt cacagcttgc
     3241 taaattgaca aaatttccgc aaccgatatt cctgatttaa cctatcttaa cgtaatttaa
     3301 cgttaaataa ctccgaagtt aattctgcag taaatttcag tttctaaatt tttctttcca
     3361 gagactgtat tttttaaaaa cctccagcgg cgcatcagtg taccagctat agccattgcg
     3421 gcgttcataa ccgatctcat cgagactctt tttctttatt ccgtcgcggt cgcagaaaaa
     3481 tggctctgct gtttcgagat catagaacct tgcccacata tcgccagcct gagggtcatt
     3541 aacaatacgc ctatccctga gtccttcggc atttgtgaaa tattcccatc taacattttt
     3601 tatcctgtgt ttatctaacc attctacagc accttcgata gctctgataa ttttcttcga
     3661 aggagtggga atttccatga gcagtaaaac tattccggcc gactcagctc cgctgaaaga
     3721 aggcagctca tacgaccttg caccagctgg tttgagagta ttctcgtcat gctgggcaca
     3781 ccatactgtc ggcttaccat tcacaatgat ctgggttttc agtatgcagt caatgccttt
     3841 gttgaatgct ttcctggttt ttgaaaggag cttattgtct gtaaaatatt ctgaagattt
     3901 tgtttgtttt cattgatatc ttttaacagt cttaaaatat tcacagtcgc attgtcatta
     3961 aatgtgatgt gtgaataata tccttttctt aacggccaga acataggcca gcctccgttt
     4021 ttatactgtg aatcaagtat aaagagcagg cctttattga aagcttcctt caattccggt
     4081 ttctcaagat aggtggcaac aacatgcaat ccctggaatg tgccggcttt tcccagcata
     4141 ccaataatgt ttttctgttg ttggatgtct tttgcaaaag gcattacggt gcgcaggaat
     4201 acagtttgca cctctcctgt tttgccggaa gatgcaatta atttaaggat ggcgttaaca
     4261 gcttctttag cattggcaac agggggatta gcttccaaaa tatccaataa cagatagatg
     4321 gtttcgaaat cagacgaagt gataagcgct ttaaatgcag cctctttgtc gtctcctgtg
     4381 ctttgtttgt aggtttcggc aatcatgtcg aatgaagctt tggatcctgt tttgattaac
     4441 aaaggatagt acaaacgttt taatgaagat gtttccattt gtttgttga
//
LOCUS       DAOSNB010000212         4481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19479_length_4481_cov_4.592634, whole genome shotgun sequence.
ACCESSION   DAOSNB010000212 DAOSNB010000000
VERSION     DAOSNB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19479_length_4481_cov_4.592634"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLG42_08575"
     CDS             complement(<1..189)
                     /locus_tag="PLG42_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31301.1"
                     /translation="MKKTALLLLLIFIVPLQLISQTRFNGLDMNMGNLFRLSDAKTRS
                     ISPENFTGEKGKGGMADPA"
     gene            424..936
                     /locus_tag="PLG42_08580"
                     /pseudo
     CDS             424..936
                     /locus_tag="PLG42_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(964..1953)
                     /locus_tag="PLG42_08585"
     CDS             complement(964..1953)
                     /locus_tag="PLG42_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOU31302.1"
                     /translation="MSKRFENKTLLYILGVLLLILLITFLVKIPREKSTLETTLVRFD
                     TAQVARIIIKPKSSEGEEFQFIKENGVWKVTQGKISSRPRPSAVSNILSDLLDIRPSG
                     LVAVDRSKWSEYDVTDSLGIRVRAENKKGKALADVVIGKFSYKPVQNPYGGYGGGSID
                     GVSYVRLYNENKVYAVDGFLTFSFTGGFDDYRDKTLVHATRSDITKISFTFPADTGFV
                     LEKKDNKWMAAGIPADSTRTSDYLSDICYLDGEKFADDFKPASSPVYKADIEGNNLLS
                     ITIKCYRQEDGKLIINSSLNPDSYFETNPDGVFGRIFKPLGYFTPSGSQQQAK"
     gene            complement(1946..3460)
                     /locus_tag="PLG42_08590"
     CDS             complement(1946..3460)
                     /locus_tag="PLG42_08590"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOU31303.1"
                     /translation="MGKTGKISTTIGLVALIVIIINILAHIYSFRLDLTENKEYTLSR
                     ATRNILRNLDRPVTITAYFSKDLPPHVINVSTGLKDMLVEYNNRSKGMVVYRFVNPNE
                     KEELEQEAIKNGIQPVMINVREKDQVKQQKAYMGAVVSMGDQKEIIPFFQPGAAMEYA
                     LSSAIKKLSVTEKPSVAFIQGHGEPALNEIAQAYSELSVLYNVEPYTINDTTPIPTKY
                     KTVAIVRPKDTIPAKDLAKLDEFLGRGGHLLLAFDRVEGDFSVPMGRPVSYGLEEWLQ
                     NKGIAVSESFIIDTKCGYVTLQQQQGNFIMTTQIQFPYLPVISKFADNPVTKGLESVS
                     LQFASPINFAGDSTKKFSPIAFSSERSGTLKAPLYFDVQRQWTQADFPVSNLVVAGIL
                     EGKLAGDADSKMIIISDGDFAVNGPQRGIQLPEDNVSLLVNAIDWLSDETGLIDLRTK
                     EVTSRPLKEIPDGRRAFLKWFNFLSPVIIILAYGLIRWQMNRNKRIKRMEVSYE"
     gene            complement(3488..4213)
                     /locus_tag="PLG42_08595"
     CDS             complement(3488..4213)
                     /locus_tag="PLG42_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOU31304.1"
                     /translation="MEKIWIITKRELNAYFDSLIAFIMLVLFLGFSGFFTWISGNDIF
                     LSGQASLRPFFSIAYWTLFFFIPALTMRMLAEENKTGTIEMLLTKPVTDRQVILGKYI
                     ATLILISVALALTFPYVITVAKLGNIDAGAVICGYLALLFISAAYTSIGLFTSSITNN
                     QIVAFLTALFIGLFFHILFQIISGGMKGLAGQVFNTLSMTVHFDSLSRGVLDLKDIVY
                     FCSIIFFGLFLTEVSLAKRNAFN"
     gene            complement(4224..>4481)
                     /locus_tag="PLG42_08600"
     CDS             complement(4224..>4481)
                     /locus_tag="PLG42_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOU31305.1"
                     /translation="QGADILRVKIEDGNPDTIFKALKTLDSVTMVEFADRQFNRFNVH
                     CKSEQVRREIFTLCVKNNWVLTELTPFETKLEDIFRELTMN"
BASE COUNT         1235 a          877 c         1002 g         1367 t
ORIGIN      
        1 ggcagggtcg gccataccgc ctttaccttt ttcaccggtg aaattctcag gacttataga
       61 gcgtgttttt gcatcagaaa gcctgaaaag gttacccatg ttcatgtcaa gaccgttgaa
      121 gcgcgtttgc gaaataagct gaagcggcac aataaaaatc agtaatagta ataatgctgt
      181 ctttttcata caggaaaatt tagagttaca ttataaaatt aggaaatttt gcaggttaag
      241 agtatgcata tgtatttttt ttaaaaacaa taatttttta ataacatgtc ggaagtggca
      301 atcaatttaa gagatgaacc tgtcagattt ataatttatt aagtggatga agtcaatttt
      361 taatagtgtg tggaaattat gttttgtttt tagtatctta aacgttttga taattaaata
      421 aaaatgaaga aaaacaaaaa atcagaaaca atcagccgca ggagctttat atcgtcatca
      481 gctgtggctg ctgcgggatt tactatcgtc ccgtcgcatg ttatttccgg tctgggttat
      541 acacctccca gcgacaagct taacattgcc ggtgtgggta ttggagggat gggtaggacc
      601 aatcttgcca atgttgctaa gactgagaac atcgtagcat tgtgtgatgt agattggaat
      661 aatgatgtac ttaaggtatt cagtacatat ccgaaagcga agcaatataa ggattaccgt
      721 gtcatgcttg acaggcagaa ggatattgat gctgtgatta ttgccacgcc cgaccacact
      781 catgcagtaa taagtatgga ggcaatgagg cggggcaagc atgtctttac tcagaagccg
      841 ctcacgcata ccgtttatga ggcaagagcc cttgcgaagg ctgccaggga atacaaggtt
      901 gcaacgcaga tgggtaacca gggtcaggca actgatggtc agagccagct ggagcctatg
      961 agactatttt gcctgctgct gagaacctga tggggtaaag tatcccagcg gcttgaaaat
     1021 gcggccaaaa accccatcgg ggtttgtttc aaaataggaa tcaggattaa ggcttgagtt
     1081 aataatcagc tttccgtctt cctgcctgta acattttatt gttatgctga gcagattatt
     1141 accttctata tctgctttgt aaacaggtga tgaagcaggt ttaaaatcat ctgcaaattt
     1201 ctcaccgtcg agataacata tatcgctgag ataatcggaa gtcctggttg aatctgccgg
     1261 aatcccggcc gccatccatt tgttatcttt tttctcaagc acaaatccgg tgtcggcagg
     1321 gaatgtgaaa cttatttttg taatgtcgct tcttgttgca tgaacgaggg ttttgtccct
     1381 gtagtcgtca aaaccacctg tgaatgaaaa agttaaaaag ccgtcgacag cataaacttt
     1441 attttcatta taaagccgaa cataagatac tccgtcgata cttccgcctc cataacctcc
     1501 ataaggattc tgaaccggtt tgtatgaaaa ttttccaatt accacatcgg caagggcttt
     1561 accttttttg ttttcagccc ttaccctgat gccgaggctg tcagtaacat catattcaga
     1621 ccatttcgac ctgtcgacgg caacaagtcc ggaaggtctt atgtcgagca ggtcagaaag
     1681 aatatttgaa acagctgagg gacgtggacg tgagcttatt tttccctgag ttactttcca
     1741 taccccgttt tctttgataa actggaattc ttcaccttcc gaactctttg gctttatgat
     1801 aattctggcg acctgagccg tatcgaatct tacaagagtt gtttcaaggg ttgatttttc
     1861 acgcggtatc ttaacaagaa atgtaattaa cagaatgaga agaagtacac ctaggatata
     1921 aagaagtgtt ttgttttcaa atcttttact catagcttac ctccattctt ttaatacgtt
     1981 tattacggtt catctgccat cttatcaggc catatgctaa gattattatt acaggcgaaa
     2041 ggaaattgaa ccatttaagg aaggcccgtc ttccatcagg tatttcttta agaggtctgg
     2101 atgtaacttc ttttgttctc aggtcgatga gtccggtctc gtccgaaagc caatcgatag
     2161 cattaaccag caggctaacg ttatcttcgg gcagttgtat gcccctttga ggaccgttta
     2221 ctgcaaaatc gccgtcggaa atgattatca tttttgaatc ggcatctccg gcaagttttc
     2281 cttcaagaat tcctgccaca acaaggttgg aaaccggaaa atcggcctgt gtccattgcc
     2341 gttgtacgtc aaagtaaagc ggagctttta aagtacccga acgttcggaa gaaaatgcga
     2401 tgggcgagaa tttctttgtg gaatcacctg caaaatttat agggctggca aactgaaggc
     2461 taacagattc gaggccttta gtaaccgggt tatcggcgaa ttttgatatc actggcagat
     2521 atgggaattg aatctgggtg gtcatgatga aattaccctg ctgttgctgg agggtcacat
     2581 agccgcattt tgtgtctata ataaatgatt ctgaaacagc tattccctta ttctgtagcc
     2641 attcttccag accgtagctc acaggtcttc ccatgggtac tgagaagtct ccttccacac
     2701 ggtcgaaagc cagcaacaga tggccacccc tgccaagaaa ttcatcgagt tttgcaagat
     2761 cctttgctgg aattgtgtct ttaggcctga ctattgccac tgttttatat tttgttggga
     2821 taggtgttgt gtcgttgata gtatatggtt caacattata cagaacactg agttcgctgt
     2881 aagcctgggc aatttcattc agggcaggct cgccatgtcc ctgaataaat gcgacggatg
     2941 gtttttcggt tacggaaagt tttttgattg ccgatgaaag agcgtactcc atggcagcac
     3001 ctggctggaa aaagggaatg atctccttct ggtcacccat tgacactact gctcccatgt
     3061 atgccttttg ctgttttacc tggtcttttt cccttacatt tatcataaca ggctggatac
     3121 cgttctttat ggcttcctgt tcgagttctt ccttttcatt tgggttgacg aaacggtaaa
     3181 caaccatccc ttttgacctg ttgttgtatt caaccagcat atctttaaga cctgttgaca
     3241 cattaattac gtggggtgga aggtcttttg agaagtatgc agttatggta acaggcctgt
     3301 cgagatttct gagaatattt ctggttgccc tgctgagtgt atattctttg ttttctgtaa
     3361 ggtcgagcct gaatgaataa atatgggcaa gtatgttaat tattatcaca atcaatgcta
     3421 cgagtccgat agttgttgat atttttcctg ttttacccat aacgtgaagt attttacgtt
     3481 taaggaatta gttgaatgca ttgcgttttg caagggatac ttcggtaagg aaaagtccga
     3541 agaaaattat cgagcagaaa tagacaatat ctttgagatc gagcactcct cttgaaagac
     3601 tgtcgaaatg aacggtcatg ctgagggtgt tgaaaacctg accggccaac cctttcattc
     3661 cacctgatat tatctgaaag aggatatgga aaaagagtcc gatgaatagt gctgttagga
     3721 atgctacaat ctggttattg gtaatgctgc ttgtgaagag tccgatactt gtatatgcgg
     3781 cgcttatgaa tagcagtgcg agatatccgc atataactgc tcctgcgtca atgttaccaa
     3841 gttttgctac tgtgataaca taggggaacg taagtgccag agcaacagat atcaggatca
     3901 gagttgcaat atatttcccc agaattacct gacggtcggt aacaggtttt gtaagcagca
     3961 tttctattgt tccggtcttg ttttcttcag ccagcattct cattgtgaga gccggtatga
     4021 aaaagaaaag ggtccagtat gctattgaga agaaaggtct aaggcttgcc tggcctgaaa
     4081 gaaaaatatc gttacccgat atccatgtaa agaatccgct gaaaccgaga aaaaggacaa
     4141 gcataataaa ggctatcagc gaatcgaaat atgcattcag ttcacgtttg gtgattatcc
     4201 agattttttc catagctaag attttagttc attgtaagtt ctctgaagat atcttcgagt
     4261 tttgtttcga acggggtcag ttctgtaaga acccagttgt ttttcacgca gagagtgaag
     4321 atttctctcc gcacctgttc cgatttgcaa tgaacattga agcggttgaa ctggcggtcg
     4381 gcaaactcga ccattgtcac tgaatcaagg gttttaagtg ctttgaatat tgtatcaggg
     4441 tttccgtctt caattttcac ccgcaggatg tcggctccct g
//
LOCUS       DAOSNB010000213         4465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19566_length_4465_cov_3.781859, whole genome shotgun sequence.
ACCESSION   DAOSNB010000213 DAOSNB010000000
VERSION     DAOSNB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19566_length_4465_cov_3.781859"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLG42_08605"
     CDS             complement(<1..306)
                     /locus_tag="PLG42_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOU31306.1"
                     /translation="MELKLKRPLAFIDLETTGINILRDRIVEISVLKVSPGGKEEWFS
                     TRVNPGMPIPAEATAVHGISDEDVAGSPGFSEIAKNLADFLEGCDLAGYNANRFDIPL
                     "
     gene            complement(308..1438)
                     /gene="dnaN"
                     /locus_tag="PLG42_08610"
     CDS             complement(308..1438)
                     /gene="dnaN"
                     /locus_tag="PLG42_08610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOU31307.1"
                     /translation="MKFVVSSSKLLGHLQSISRVISSKNTLPILDNFLFNLSDNELEI
                     TASDLETTLITRLKIENAEGKGMIAVPARLLLETLKEFSDQPLTFDINLDTLAVTIIS
                     ENGKFNAVGQNGIDFPVLPAIKKEKKFSYTMGSDVLLAGINKTIFATADDELRPVMGG
                     ILVEASPEKVTFVASDAHKLVRYQRTDAKSDAPCSFILPKKPAGLMKNILPKEEGQVL
                     IEFDDKNAFFTLTDYRIVCRLVEGNYPNYNSVIPRNNPRKLIVDRAGFYNTLKRVSVF
                     SNQASNLVRLQLTGNQVIVSAQDIDFSISAVERIKCQYEGDEMEIGFKAAFLLEILAN
                     LSSPEVIIELADPTRAGLFLPSETENESEDLLMLLMPMMINA"
     gene            complement(1617..2603)
                     /locus_tag="PLG42_08615"
     CDS             complement(1617..2603)
                     /locus_tag="PLG42_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31308.1"
                     /translation="MKKIGNLSVYFAIILVCAVALYLLFRQRFPFGKGNTDFAVEEKT
                     EVTRIVFYERNMKLVLLKNNEDEWVIGNNLPARDEAVRFLIKVLKEMKVKSPVTSETF
                     SEEIVKKKIEPVKIHVYEKRRLKKSFFVYHTDSNTYGNMMKMRPSSKPYVMFVPGYET
                     DIGMYFTANNLFWQPFIVFNFMPSEIEKVTLQNYADTSASFRILCQDKDISLLTAEGI
                     AASCCDTTRVRRYVSYFTYIPFERWATELTGEKKEEIKSSVPLYRIELKTREGKNVNL
                     TIWQKFKTLDDGKEIIDNDRVWGQTGDIDEIFVMRYFDIDPILKKISYFCGE"
     gene            complement(2600..4327)
                     /gene="gldG"
                     /locus_tag="PLG42_08620"
     CDS             complement(2600..4327)
                     /gene="gldG"
                     /locus_tag="PLG42_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOU31309.1"
                     /translation="MEEKKLKNIFKNDVRLKSWADFLIISIAALLIAAASGFIRLRID
                     LTEDRRYSLSEQTRKILGGLKEDIYIQVFLEGEMPVLFKKLRRSVRELLDEFRIASHR
                     RIDYEFINPAEGSDPAAREKFQEELIAKGISPVNVMMKDREGGSSQRLIYPGMIINYN
                     NAEVPVNFLKNNPALSPEVNLLHSAEGLEYELIQTIRTLTADTVYKIAFIEGHGEVPE
                     IEVADLVLNLEKYFTVDRGTPGGKPGVLDNYSAVVVAGPMKNIDEKDLFVIDQYIMKG
                     GRVLWLYEEVDVNTDSLVYGETVALYKPLGLQEILFKYGVRVNPVLVQDLECMLIPIK
                     VITGGTQQQYVPMPWVYYPLLVPSPSHPVTRNLNRVIGKYVSYIDTVGRDPEIKKEIL
                     LTTSQRTRTVSPPVYITLKDVDRMPDESQFNKSHLPVAVLLSGKFKSAFRNRIVPGLT
                     DKGDYKIITESKPAKMIVIADRDIIRNEVRRTASGEMPLPLGQDRYTMQTFGNRDFLI
                     NCVNYLTDDIGLMELRSRELKLRLLDKKLINEKRVLIQAVNTCGPILLIIAGGILYTL
                     IRRKMYVRK"
BASE COUNT         1231 a         1013 c          837 g         1384 t
ORIGIN      
        1 caatggtata tcaaacctgt tagcgttata gccagcaagg tcgcagcctt caagaaaatc
       61 tgcaagattt tttgctatct ccgaaaaccc tggtgaaccg gcaacatctt catcgctaat
      121 accatggaca gcggtagctt cagctggtat aggcatacca gggttaaccc ttgtcgaaaa
      181 ccattcctct tttccgccag gagatacttt gagcacagaa atttctacta tcctgtctct
      241 taaaatattt atgccggtgg tttcgaggtc aataaaagcc agaggccttt tgagcttcag
      301 ttccatctca tgcgtttatc atcatgggca tcagaagcat cagcaggtcc tccgattcat
      361 tctctgtctc ggatggcagg aagagccctg cacgtgtcgg atcggctaac tctataataa
      421 cttctggcga cgaaaggttt gccagaatct ccagcaggaa agctgccttg aagccaatct
      481 ccatttcatc tccctcgtac tggcatttta tccgttcaac agctgatatt gaaaaatcaa
      541 tgtcctgtgc agaaacaata acctgattac cagtaagttg aagcctgacc agattgcttg
      601 cctggtttga gaaaacggag acacgtttca gtgtgttgta aaatcctgcc ctgtcaacta
      661 taagctttcg aggattattt ctgggaatta ctgaattgta attgggataa ttaccttcaa
      721 caaggcggca aactatcctg taatcggtga gtgtaaaaaa tgcatttttg tcgtcaaact
      781 ctattaacac ctgtccttct tctttcggaa ggatattctt catcaatcca gcaggtttct
      841 tgggcagaat gaatgagcag ggggcatcag actttgcatc ggtacgctga tacctcacca
      901 gtttatgtgc gtcggatgca acaaatgtta ctttctccgg tgatgcttcc acaagaattc
      961 cgcccatcac aggtctcagc tcatcatcgg ctgtagcaaa gattgttttg tttatgcctg
     1021 caagcaatac atcggaaccc atcgtatagg aaaacttctt ttctttttta attgcaggca
     1081 gaaccgggaa gtcaattccg ttctgaccga ctgcattgaa tttaccgttt tccgagataa
     1141 tcgttactgc aagtgtgtcg aggttgatgt caaaagtaag gggctggtcg ctgaactcct
     1201 ttaaagtctc aagtaaaagc ctggcaggca cagctatcat tcctttacct tccgcattct
     1261 cgattttcaa ccgtgttata agtgttgtct caagatcgga cgctgttatc tcaagctcat
     1321 tgtcactcag attaaaaaga aaattatcaa gaattggcaa tgtgtttttt gagcttatta
     1381 ctctgcttat cgactgcaga tgtccgagaa gtttgctgct tgatactaca aatttcatat
     1441 tattgctttt aacgtttaac aatattttat ataaaattta tcatttcacc tttcctcctt
     1501 ccctccgggc gaaagaagga taaccggtca attcaataat aatggcactg aatatcaatg
     1561 cagtgtatta ttttctccgg tctatcaata ttacaaaaaa acataataac atcaaatcat
     1621 tcgccgcaga aatatgaaat cttcttcaaa atcgggtcta tatcaaaata ccgcattaca
     1681 aatatttcat caatatcgcc ggtctgtccc cataccctgt cgttatcaat gatctctttg
     1741 ccatcatcta gagttttaaa tttctgccat atagtcagat taacattttt tccttccctc
     1801 gttttaagtt cgattctata gagaggtact gaagatttaa tttcctcttt cttttcacct
     1861 gtcaattccg ttgcccatct ctcaaaagga atatatgtaa agtaagagac atatctcctt
     1921 acccttgtcg tgtcacagca tgatgcagct attccttcag ccgtcagcaa tgatatatct
     1981 ttatcctggc ataatatcct gaatgatgct gaagtatcgg cataattctg cagagtaact
     2041 ttctcaattt cagaaggcat aaagttgaaa acgataaaag gctgccagaa aagattgttt
     2101 gccgtgaaat acatacctat atctgtttca tagccaggta cgaacatcac ataaggtttc
     2161 gacgacggcc tcattttcat catattgcca taggtattgg aatcggtatg gtagacaaaa
     2221 aaacttttct tcagccttct tttctcatag acatgaattt ttacaggctc aattttcttt
     2281 ttgacaattt cttcagaaaa agtttcagat gttacaggtg attttacctt catctctttc
     2341 agaaccttta taagaaatcg gaccgcctcg tctctcgcag gaagattatt accaataacc
     2401 cattcatctt cattgttttt taaaagcaca agcttcatat ttctttcata gaaaacgatc
     2461 ctggtaactt ccgtcttctc ttccacggca aagtctgtgt taccttttcc gaaaggaaat
     2521 ctttgcctga aaagcaggta taatgccact gcgcaaacaa gaataatggc aaaatatact
     2581 gacagattac ctatcttctt cattttctga cgtacatttt ccttcggatc agggtataaa
     2641 gaattcctcc tgcaatgata agtaaaattg gtccacaggt attgacagcc tgtataagaa
     2701 cccttttttc attaatgagt tttttatcga gaagcctgag tttcagctcc cttgagcgta
     2761 gttccatcag acctatatcg tcggtgagat aattaacaca attaataagg aaatccctgt
     2821 taccgaaagt ctgcattgta tacctgtcct gcccgagtgg cagtggcatt tcacctgaag
     2881 ctgttcttct tacctcatta cgtataatat cacggtcggc aataacgatc attttagcag
     2941 gtttgctctc ggtgattatt ttataatcgc ccttatctgt caggccgggc acaattctgt
     3001 ttctgaaagc tgacttaaac ttccctgata gaagcactgc cacaggaaga tgcgacttgt
     3061 taaactggct ttcatcaggc attctgtcga catctttcag ggttatgtat acaggaggac
     3121 tcacagttct agtacgctgg cttgttgtga gcagaatttc cttttttatc tcaggatctc
     3181 ttcccactgt atcaatataa cttacatatt ttccgataac tctgttaagg ttacgggtaa
     3241 cagggtggga aggtgaaggt acgagaagcg gataatagac ccatggcatg ggcacatact
     3301 gctgctgagt tccaccggta ataaccttta tggggatgag catacattca aggtcctgta
     3361 caagtacagg gtttaccctg acgccgtact tgaaaagtat ctcctgcaag ccaagcggct
     3421 tatacagagc aacggtttca ccgtagacaa gactatcggt attaacatct acttcttcat
     3481 aaagccacag gactctccct cctttcatta tatactggtc tatcacaaac aggtctttct
     3541 cgtcaatatt tttcatggga cctgcaacca ccaccgctga ataattgtcg agcacaccgg
     3601 gttttcctcc tggtgttccc ctgtcaactg tgaaatattt ttccagatta agcacgaggt
     3661 cggcaacttc aatctcgggt acttcaccat ggccctcaat aaatgcaatt ttatagacag
     3721 tgtcggctgt aagtgtccga atggtctgta taagttcata ttcaagtcct tcagctgaat
     3781 gaagcaggtt aacctcaggt gatagagcgg ggttgttctt aagaaagtta accggaactt
     3841 cagcgttgtt gtagttaatt atcatgcccg gataaatgag tctctggctc gaacctccct
     3901 ccctgtcttt catcataaca tttaccggtg agataccctt tgctatcagt tcttcctgaa
     3961 atttctccct tgcagccgga tcgcttcctt ctgcaggatt aatgaattcg tagtcaatcc
     4021 ggcggtgtga tgctattctg aactcatcca gcaattccct taccgacctt cgcagtttct
     4081 tgaaaagaac aggcatctct ccttcaagga aaacctgtat gtagatgtct tccttaagtc
     4141 ctcccagtat ctttctggtc tgttccgaaa gcgaatagcg tctgtcttcg gtaaggtcaa
     4201 tacggaggcg aataaaaccc gacgcagcgg caatcagcaa agctgcaatg ctgattataa
     4261 gaaaatcagc ccagctctta agccgtacat catttttaaa aatatttttc agcttcttct
     4321 cttccatttt cttgaaagta atgaaagcct tgatgcttca ttgaatataa ctgctccagt
     4381 cgcgaaatat accagatccc ggaagtcgat aacacctctg ctcatcgacc tgtaatgttc
     4441 attaacccca agaagggata caaat
//
LOCUS       DAOSNB010000214         4408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19882_length_4408_cov_4.568344, whole genome shotgun sequence.
ACCESSION   DAOSNB010000214 DAOSNB010000000
VERSION     DAOSNB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19882_length_4408_cov_4.568344"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLG42_08625"
     CDS             <1..1597
                     /locus_tag="PLG42_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31310.1"
                     /translation="TGTPMTCFDISGNRIIAGTNRGILTIDLKTRKPEGGINSKIPFT
                     EITVVRVIDGTPWLGSVKGAMKLRKDGRFDYYSSERWLPSDTIVDITKGPDNSVLILT
                     TAGLSKICFEEITLEDKALFYEKQVRQRHIRHGLNATLALARKGDLSSGSLEDSDNDG
                     LWTGLYLASQTFRYSVTRSAEALQNVRESLEAMERLYTINPVPGFPARSFERIGYPVH
                     DPERWLPSPEEGWMWKSTTSSDEAIVHMFAFGVVAELIEEPFIRSKAVKLIDTLMSHI
                     VKNNFYLIDIDGKPTRWGRWNPEYVNALHPSVGDRKLNSSNIISMLQTAYHFTGKKKY
                     HDAAFYLMNEHGYLENLMRPMKEIGRAPADADETSRMLSDGWNHSDDEMYYCGYWGLY
                     RYAFNDTLKKKFRQAIIDHWETEKPEKEALWNIMTFMTGKKDCGLDDAVWYLKEYPLD
                     LITWTIRNSHRNDIEKIPDNFRNQTINEVLPPDELPVTRHNSNRFTLDGGNGGLSEMS
                     AGDIWLLPYWMGRYLGVIGSKKN"
     gene            1625..3178
                     /locus_tag="PLG42_08630"
     CDS             1625..3178
                     /locus_tag="PLG42_08630"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOU31311.1"
                     /translation="MKKIMKFIGKLLLVAVIVLIVSIYSNLKDRHRGYSADLKIVSAE
                     EMPLMAGFSAVTITPDVPDRWTDADGDAAYRPRKGDTFTDGNGNGRFDPVWIAGFGQA
                     RAANGIHDDLWARTMVISNGKTNLAIVALDAIGFMNNHVIEIRKKLQKDLKLDYIVIT
                     STHTHEGPDLLGLWGKSPLHSGVNKKYLEYVNERIIKSVVFALQNIRPARLEVSEDPE
                     GAAHLVKDTREPQVFDSGLRIIRAVDTETGLTLGTLIAWGNHPETLWGKNLLITSDFP
                     HYLREYVEKGIYRGDSLIIKGTGGICLYVNGAVGGLMTTHPDLAVKDPFTGEEFKTPS
                     FEKADAQGKQLALLALRAMENPDTRIEKAGISIIAKTVYLPVDNKLFRLGAALGILNR
                     EFAGWMKMRSEVSVFKIGPIQFATLPGEVYPEIINGGVEAPPERDFLVDPVEVPPVRD
                     MMKEKHKFILGLANDEVGYIIPRSQWDTRKPFAYGKEKAQYGEINSLGPETAPLLHSA
                     LKNIVEAIQ"
     gene            complement(3347..>4408)
                     /locus_tag="PLG42_08635"
     CDS             complement(3347..>4408)
                     /locus_tag="PLG42_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31312.1"
                     /translation="IYNDWRVMLEKEKSIDAVVIGTPDHNHAPIAAAFMKEKKHVYCE
                     KPLAKTIYECRRLAEIAKETGVVTQMGNQGHAAEGSRRTVEWIQSGVIGLVREVHLST
                     NRPIGFWPQGDIKRPAGTTPPKNLNYDVWLGPAPQKPYHPDTVHFYWRGLWDYGTGAM
                     GDMGAHIFDAPVWALNLGYPTRIHATSTPFNDEYLPLAEMVIYEFPARGYMPPVKVMW
                     VDGGLRPPRPDTLEPGRAVRDAVYYGDKGIIMHGSHGAMPELVPADPSFKGPEPWLPR
                     TGNIFEDWIDAIKNNRKSCNDFSIAAKLTEIMLLTNIAVKMQRRNITLEYDAANMKIT
                     NLPEANELFHYEYRKGWSL"
BASE COUNT         1248 a         1033 c         1052 g         1075 t
ORIGIN      
        1 cacaggcact cccatgacat gtttcgatat cagtggaaac cggataattg caggcacaaa
       61 ccgtggaatt ctgacaattg atctgaaaac cagaaagccc gaaggcggga ttaacagtaa
      121 aatacccttt actgaaatta ctgttgtgcg tgtgattgac ggcaccccct ggttgggctc
      181 tgtaaaaggt gcaatgaaac ttagaaagga cggtaggttc gattattatt catccgaaag
      241 gtggcttcct tccgatacga ttgttgacat aacaaaaggt cctgacaatt ctgttctcat
      301 acttacaact gccggattgt caaaaatatg cttcgaagag ataacacttg aggataaagc
      361 cctcttttat gaaaaacagg tacggcagcg acatatacgg cacggcctta acgccaccct
      421 tgcccttgca cgtaaaggtg acctcagttc gggctcactg gaagattctg acaacgatgg
      481 cctctggaca ggcctttacc tcgcctccca gacattccgt tattcagtta cccgctctgc
      541 agaagcactg caaaacgtga gagagtcgct tgaagccatg gaaagactat ataccatcaa
      601 tccggtgcct ggcttccctg cacgctcttt cgaaagaatc ggctaccctg tgcacgaccc
      661 cgaacggtgg cttccatcac cggaagaggg ctggatgtgg aaatcaacca caagcagcga
      721 cgaagcaata gtccatatgt ttgccttcgg cgtggtggca gaactgattg aagaaccatt
      781 tatcaggagt aaggccgtaa aacttattga cactttaatg tcgcacattg taaaaaataa
      841 tttttatctg atcgacatcg acggcaaacc tacacgctgg gggcgctgga accctgaata
      901 cgtcaacgca ctccatccct cagtgggcga ccgcaagctc aattcatcga atatcatctc
      961 aatgctgcag acggcttacc atttcaccgg aaagaaaaag taccacgatg cagcctttta
     1021 tctcatgaat gagcatggct accttgaaaa cctcatgcgt cctatgaaag aaataggacg
     1081 cgctccggct gatgccgacg aaacaagccg catgctctcc gacggctgga accattccga
     1141 cgacgaaatg tactactgcg gctactgggg actttatcgc tacgccttca acgatacact
     1201 aaagaaaaag ttcaggcagg caataattga ccactgggaa acagaaaaac ccgaaaaaga
     1261 agcactgtgg aatataatga catttatgac cggcaaaaag gactgcggcc tcgatgatgc
     1321 agtctggtac ctgaaagaat acccgctcga ccttataaca tggacaatca gaaacagcca
     1381 cagaaatgat attgaaaaaa tacctgacaa cttcaggaac cagacaataa atgaagtttt
     1441 accacccgat gaacttcctg ttacacgaca caactcaaac agatttacac tcgacggggg
     1501 caatggcgga ttgtcggaaa tgagcgcagg cgacatctgg ctgctaccct actggatggg
     1561 aagatatctg ggggtaatag gaagcaagaa aaattgaaaa atatacctat aaaacaatat
     1621 cctcatgaaa aaaatcatga aattcattgg caaactgttg ttagtagcag ttattgttct
     1681 gatagtaagc atttattcca accttaaaga caggcacagg ggttacagtg ccgaccttaa
     1741 aatagtttct gctgaggaaa tgcctcttat ggcaggcttc tcggctgtca caataacacc
     1801 cgatgtcccc gaccgctgga ccgatgccga cggtgatgcg gcctacagac ctcgcaaagg
     1861 tgacaccttt accgacggaa acggcaatgg ccggttcgac cccgtatgga ttgcaggttt
     1921 tggtcaggca agagcggcaa acggaataca cgacgaccta tgggcaagaa caatggttat
     1981 cagcaatggt aaaacaaacc tcgccattgt ggcgctggat gctatcggat ttatgaacaa
     2041 ccatgtaata gagataagga aaaagcttca aaaagactta aaactcgatt atattgtaat
     2101 cacatcaacc catactcatg agggacccga ccttcttggc ctatggggta aatcaccact
     2161 gcacagcgga gtaaataaaa aatatcttga atatgtaaat gaacgtataa taaaatctgt
     2221 cgtttttgcc ctgcagaaca taagacctgc acgtcttgaa gtatctgaag atcctgaagg
     2281 agcggctcat ctggtaaaag acacccggga gccgcaggta tttgactccg gactgagaat
     2341 aataagggcc gttgacactg aaaccggttt aacacttggt acactgatag catggggcaa
     2401 ccatcccgag acactatggg gtaaaaacct tcttataact tccgatttcc ctcactatct
     2461 gagagaatat gtcgaaaaag gcatttacag aggtgacagc ctcatcatca aaggaaccgg
     2521 aggtatttgc ctgtatgtga acggagcagt gggagggctt atgacaactc atcccgatct
     2581 tgccgtaaaa gatccgttta caggtgaaga attcaaaaca ccatcattcg aaaaagctga
     2641 tgctcaggga aagcaacttg cattactggc tctcagggca atggaaaatc ctgacaccag
     2701 gattgaaaaa gcaggtatct caataattgc aaaaactgtt tacctgcctg ttgataataa
     2761 gcttttcagg ctgggagctg ctctcggtat tcttaacagg gagtttgcag gatggatgaa
     2821 aatgcgttca gaggtctctg tcttcaaaat aggacctatt cagttcgcca cgcttcccgg
     2881 tgaagtctat ccggaaataa ttaacggagg cgttgaagca ccaccggaaa gagatttcct
     2941 ggtcgatcct gtagaagtgc ctcctgtcag ggatatgatg aaagagaaac acaaattcat
     3001 tcttggactg gctaacgatg aggtgggtta tattataccc aggagtcagt gggacacccg
     3061 taaacctttt gcatacggaa aagagaaggc acagtatggt gaaatcaact ctctggggcc
     3121 agaaacagca cccctgctcc actctgccct gaagaatata gttgaagcga ttcaatgata
     3181 aaataaaagg gctgtctcaa aagcctcttt tttgttttca cccctgaatc ccctgagagg
     3241 ggacttatta caatacaatc tgtaatattt tagtttttat gtttttatta aacttaccta
     3301 ttatacatct tttgagacag cccccagagg gtgaagtaat aaatctctaa aggctccacc
     3361 cttttctgta ctcgtagtgg aacagttcgt ttgcctcggg aagattggtt atcttcatat
     3421 ttgcggcatc atattcgagg gtgatatttc tcctctgcat cttcaccgcg atgtttgtaa
     3481 gcagcattat ttcagtcagt tttgctgcga tggagaagtc gttgcacgac tttctgttat
     3541 tcttgatagc atcgatccag tcttcgaaaa tattgccggt tcgaggcagc catggttcgg
     3601 gtcctttgaa tgatgggtcg gcgggcacga gttcaggcat tgctccgtgg cttccgtgca
     3661 tgattatacc tttgtcgccg tagtacacag catctcttac tgcgcggcca ggctcgagag
     3721 tgtcggggcg tggaggcctc agtccgccgt caacccacat aaccttgacc ggaggcatat
     3781 agccgcgggc ggggaactcg tagatgacca tctcggcgag cgggaggtac tcatcgttga
     3841 acggcgtgct ggtggcatgt atcctggtag gatagccaag gttgagtgcc cagacaggtg
     3901 catcgaagat atgagccccc atgtcgccca tggcgcctgt gccataatcc catagtccgc
     3961 gccagtagaa atgcacggtg tcggggtggt agggtttctg gggtgccgga ccaagccata
     4021 catcgtagtt aagatttttg ggcggggtgg ttccagcagg ccttttgata tcaccctgcg
     4081 gccagaagcc tatagggcgg ttggtcgaaa gatgcacctc cctcacaaga cctatcacac
     4141 ccgattgtat ccattcaact gtgcggcgtg agccttcggc agcatgaccc tggttaccca
     4201 tctgtgtgac aacacctgtc tcctttgcta tctcggcaag acggcggcat tcatagatgg
     4261 tctttgcaag aggcttctcg caatatacgt gtttcttctc cttcataaag gctgcggcga
     4321 taggcgcatg gttgtggtcg ggtgtgccaa taacgacagc atcaatggat ttttcttttt
     4381 cgagcatcac gcgccagtcg ttatagat
//
LOCUS       DAOSNB010000215         4404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19907_length_4404_cov_4.014256, whole genome shotgun sequence.
ACCESSION   DAOSNB010000215 DAOSNB010000000
VERSION     DAOSNB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19907_length_4404_cov_4.014256"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2256
                     /gene="thrA"
                     /locus_tag="PLG42_08640"
     CDS             <1..2256
                     /gene="thrA"
                     /locus_tag="PLG42_08640"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOU31313.1"
                     /translation="ERHLETARLLLPVENHPRVMAEIMSLCNELSDIIKGAVLLSEVT
                     PRTKDLVLSFGERLSAYLLSEVLRPYIPDTLYTDAREIIRTDSDFGKAKVDFKITENL
                     VRKYFSEHKGLKVITGFIGSDPNGQTTTLGRSGSDYTATIVAYSVDAESVEIWSDADG
                     VMTADPHFVPEARSIEKLSYSEAMELSHFGARILFPLSLHPAMLKQIPVRVKNTFNPG
                     HPGTLISRETCKDNGIVKGITSLRNISIVNVEGSGMVGVAGISARLFTALSQNRISVI
                     LISQASSEHSICIAVKQEDAPGACAILREAFSAELASGLISSVNNEDGLAIVAVVGEN
                     MRHVPGVAAKVFNPLGRNGINVKAISQGSSELNISFVISESDLRKGLRILHQALFSRE
                     VRRLHIYMAGTGAIGSRLMEMIHHQQDSLLSQKIELKICGLINTRKMLLSEDGIDPGM
                     WKTYFNNAGVRAKLSEFVGGLLSHNFENSIFIDATASDEPVVYYEKLLSSNVSIVAAN
                     KRANTGRQSEYNSLKINARKRNVSFNYETNVGAGLPVINVIRNLQAGGDEIIKIEAVL
                     SGTVNWLLSEYDGKITFSELVRLAREKGITEPYPGDDLLGLDVARKCLILARECGLQL
                     EPEEVDVEPVLPAPVPSKGTVEELLREIEKQEHLIFSRHIRTTEEGKKLRYVALIENG
                     EAKVRITKTAENHPFFSLRGSENCLIFTTKYYSQYPLVVKGPGAGVDVTSAGLLADIV
                     RIAETVRLQGN"
     gene            2284..3240
                     /locus_tag="PLG42_08645"
     CDS             2284..3240
                     /locus_tag="PLG42_08645"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOU31314.1"
                     /translation="MIVHKSVTAFAPATVANISCGFDIMGFALEGEGDKVTVEVDDSP
                     GREVISITGKYAGLVPQEPGKNTAGVAINALLKAAGKENISVKITLEKNLPPGSGMGS
                     SASSAAAAVYALNRLFGNPFRTIDLIPFAMEGERAACGTAHADNVAPSLLGGFVLIRS
                     YEPLDIIQLKTPIDLYCVVIYPHIEVKTSDARRVLRRDFPLNVVTRQSANTAAFTAGL
                     LTGDYSLLSRSMNDLLAEPRRTMLIPGFAAAKKSALDAGAIGCGISGSGPSVFAFSSD
                     KNRADKIADAMQKAFREAGLNSDRIISSLNAPGAKVINEVDF"
     gene            3255..>4404
                     /gene="thrC"
                     /locus_tag="PLG42_08650"
     CDS             3255..>4404
                     /gene="thrC"
                     /locus_tag="PLG42_08650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOU31315.1"
                     /translation="MLYYNIDKPSRKVSLVEAVMRSASEERALYMPEKIPSLDSSFFE
                     HFSEMTLQETAYRVTEPVFGEDIPAEELRNIIERALNFDIPLVKLFPDIYVLELFHGP
                     TFAFKDVGARFLAGMLDYFAGSINNEIKVLVATSGDTGGAVANAFAGKKNIKVVILYP
                     SGRVSFIQEQQLTKAGENILALEIKGDFDDCQKLVKQAFADSKLNEEIVLTSANSINF
                     ARLFPQSVYYFYSFAQLKKNDYPVVFSVPSGNFGNLTAGLIAKRMGLPAVKFIASTNI
                     NRSVPLYLESGKFVPHHSFPTISNAMDVGNPSNFRRILDLYSNDIEKIRADISGFWFT
                     DEKTRQAMKELYTRYSYIADPHTAVAYLGLKEYMKNNSCTGIFLATAHP"
BASE COUNT         1290 a          932 c         1046 g         1136 t
ORIGIN      
        1 gagaggcacc ttgaaactgc ccgtttactg ctgccggtag aaaaccatcc gcgggtgatg
       61 gctgagataa tgtcactctg caacgaactg tcagatatta tcaaaggtgc tgtactgctg
      121 tcggaagtga caccccgtac aaaagacctt gtactcagct tcggcgaacg cctttcagca
      181 tacctgctct ctgaagtact tcgtccttat atccctgaca ctctttatac cgatgcacgg
      241 gaaattatca gaaccgattc ggacttcgga aaagcaaagg ttgacttcaa aataacagaa
      301 aatcttgtaa gaaagtattt ttctgaacat aaaggactta aagtaataac cggttttatc
      361 ggctccgacc cgaacggaca aaccaccacc cttggcagga gtggctcaga ttacactgcc
      421 accatagtgg catattcagt tgatgctgaa tcggttgaga tatggtccga tgccgacggt
      481 gtaatgacag ccgatcctca ctttgttcct gaggcgagaa gtattgaaaa actttcctat
      541 tctgaagcga tggaattgtc acacttcgga gcaagaatcc tgtttccgct atcgcttcac
      601 cctgccatgc ttaaacagat acctgtgagg gttaaaaata ctttcaaccc cgggcatccc
      661 ggaacactta tcagcaggga gacctgcaaa gacaacggta ttgtaaaggg aataacatcc
      721 ctcagaaaca taagcatagt taatgttgaa ggaagcggga tggttggagt tgccggaata
      781 tctgcaaggt tattcaccgc attgtcgcag aacaggataa gcgttatcct tatcagtcag
      841 gcatcctcgg aacattcgat atgtattgcc gtaaagcagg aagatgcccc tggtgcctgt
      901 gccattctca gggaagcctt ctcggccgaa cttgcatctg gactgatatc atctgtaaac
      961 aatgaagatg ggcttgcaat tgtggcggtg gttggtgaaa acatgcggca tgttccgggt
     1021 gtggcggcaa aggtgttcaa tcccctcggg cgtaatggca taaatgtcaa agccatctca
     1081 caggggagct cagagttgaa tatatcattt gtcatcagtg aaagtgacct ccgtaaaggc
     1141 ctccgtattc tgcatcaggc actcttcagc cgtgaagtgc gccgccttca tatatatatg
     1201 gccggaaccg gcgcaattgg tagccggctg atggagatga tccaccacca gcaggatagc
     1261 ctcctttcac aaaagattga actgaagata tgtggactta taaatacaag gaagatgctc
     1321 ctaagcgaag atggaataga ccccggaatg tggaaaacct attttaataa cgccggcgtc
     1381 agggcaaaat taagtgagtt tgtcggggga ttactgtcgc ataattttga gaacagtata
     1441 tttattgatg ctacggcaag tgatgagcca gtggtttatt atgaaaaatt gctttcatcg
     1501 aacgtttcga ttgttgcagc aaataaacgc gccaataccg gaaggcagag cgaatataac
     1561 agtttaaaaa taaatgcccg gaaacgtaat gtctcgttca actatgaaac caatgtaggc
     1621 gccgggctgc cggttattaa tgtcatccgt aacctgcaag caggcggtga tgaaataatt
     1681 aaaatcgaag cagtgctatc cggcactgtc aactggcttc tttcggaata tgacggaaag
     1741 ataaccttca gcgaacttgt acgcctggca agagaaaaag gtatcactga accttatccg
     1801 ggcgatgacc ttctgggact tgatgtggca aggaaatgcc ttattcttgc cagggaatgt
     1861 ggacttcagc ttgagcctga agaggtagat gtagaacccg ttcttccggc acctgtacct
     1921 tcaaaaggaa ctgtggaaga gctactcaga gagattgaaa aacaggaaca tctgatattc
     1981 agtcgacata ttcgcaccac tgaagaagga aagaaattgc ggtatgttgc tttgattgaa
     2041 aacggtgaag caaaagtcag aataaccaaa acggcagaaa atcatccctt cttttcactc
     2101 agggggagtg agaactgcct gatttttaca acaaaatatt acagccagta tccgcttgtg
     2161 gtgaaaggac cgggtgcagg tgttgacgtt acttcagcag gtctgctggc agatatagtt
     2221 agaatagcag aaacagtaag attacagggt aattaaacag ggtaattaaa aaagtttaat
     2281 gaaatgatag tacataaatc tgttacagcc tttgcacctg ccaccgtggc aaacatctcg
     2341 tgcgggtttg atattatggg ttttgctctt gaaggcgaag gcgacaaggt tacggttgaa
     2401 gttgatgatt caccaggaag agaggtaatt tccattactg gaaaatatgc tggacttgtg
     2461 cctcaggaac caggtaagaa cactgccggg gtggcaataa atgcattgct taaggctgcc
     2521 ggaaaagaga atataagtgt taaaattaca cttgaaaaga accttcctcc tggaagtggg
     2581 atgggaagca gtgcttcgag tgctgcagca gcagtgtatg ctctcaacag gctcttcggg
     2641 aatcccttca gaacgattga cctgattcct tttgccatgg aaggagagag ggctgcctgc
     2701 ggaacagccc atgccgataa tgtagccccc tcacttctgg gaggcttcgt tctcatccgc
     2761 agctatgaac ctctcgatat cattcaactt aaaacaccta tagaccttta ctgtgttgtc
     2821 atctatcctc atatagaagt aaaaacttct gatgcaaggc gtgtgcttcg gcgcgacttt
     2881 cctttaaatg ttgtgacacg gcaaagtgca aatacagcag catttacagc tggtttacta
     2941 acaggagatt acagccttct gagtcgctcg atgaatgacc ttctggcaga accaagaagg
     3001 acaatgctga tacccggttt tgctgctgct aaaaagtcgg cccttgatgc cggtgccata
     3061 ggctgcggta tctcaggctc tggaccttca gtattcgctt tctcatccga caaaaaccgg
     3121 gcagataaaa ttgctgatgc aatgcaaaaa gcattccgtg aagccggact gaactctgac
     3181 agaataattt cttcactaaa tgcaccgggt gcaaaggtta ttaatgaagt agatttttaa
     3241 tatgcagata aacaatgctt tactataaca tagataaacc ttcccggaaa gtttcactcg
     3301 ttgaagctgt aatgagaagt gcctcggaag aaagagccct ctatatgccg gagaagatac
     3361 cttcactgga cagttcattt tttgaacatt tcagtgaaat gactcttcaa gaaaccgcat
     3421 acagggtgac tgaaccggtg tttggcgaag atataccggc tgaagagctt agaaatatca
     3481 ttgaaagggc cttgaatttt gacataccgc ttgtaaaact ctttccggat atttatgtgc
     3541 tggaactgtt tcatggtcca acctttgcat tcaaggatgt gggagcaagg tttcttgcag
     3601 gaatgcttga ctattttgca ggaagcataa acaatgaaat aaaagttctg gtagccactt
     3661 cgggcgatac gggtggggcg gttgctaatg ctttcgcagg aaaaaagaac atcaaggtgg
     3721 taatactata tccttcaggc agagttagtt ttattcagga acagcaactg acaaaagccg
     3781 gagagaatat tcttgccctc gaaattaaag gtgatttcga cgattgccag aagcttgtaa
     3841 aacaggcctt tgccgacagt aaactgaatg aggaaattgt attgacttcg gcaaattcga
     3901 taaattttgc cagactcttc ccacagtcgg tatattattt ttactctttc gcacagttaa
     3961 agaagaatga ctatcctgtt gtattcagcg ttccgagcgg taacttcgga aaccttacgg
     4021 cagggcttat tgcaaagcgc atgggtctgc cggccgtaaa gttcatcgcg tcaacaaata
     4081 ttaacaggtc agttccgcta tatcttgaat cgggaaaatt tgtaccgcat cattccttcc
     4141 ctacaatatc aaacgcgatg gatgttggga atccgagtaa cttcagacgc atcctcgatc
     4201 tgtatagcaa cgatattgag aagattagag ctgacatatc cggtttctgg tttaccgatg
     4261 agaagacacg tcaggcgatg aaggaacttt ataccagata ttcatatatt gctgatccgc
     4321 atacggcagt ggcatatctc ggactgaaag aatacatgaa aaacaattcc tgcaccggca
     4381 ttttccttgc tactgctcat ccgg
//
LOCUS       DAOSNB010000216         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_19923_length_4400_cov_4.461910, whole genome shotgun sequence.
ACCESSION   DAOSNB010000216 DAOSNB010000000
VERSION     DAOSNB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19923_length_4400_cov_4.461910"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLG42_08655"
     CDS             complement(<1..168)
                     /locus_tag="PLG42_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU31316.1"
                     /translation="MSIYSSSVKRPVTTILVFVAMIVIGLYSLVQLPVDLYPEIELPY
                     ITVLTTYPGTSA"
     gene            complement(190..1227)
                     /locus_tag="PLG42_08660"
     CDS             complement(190..1227)
                     /locus_tag="PLG42_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU31317.1"
                     /translation="MKRFGTLIAVVISGVILVSCTSTPGETTGESKTAAPVKVTPLTR
                     TKIAKTIDYSATIQAFEEVNVAPSSPGRIEKIYVEAGDRVNKGDNLFLMDRTQLYQLK
                     LQLSSLEKDLWRLDTLLKAGSAKQQQYDQLKTQYEVTKTNVEFLEQNTLLKAPFQGIV
                     TGKYYEDGEMYSGAPSMSGKPAVVTLMQINPLKVIVSLSEQYYPLIKKGMEAVITADV
                     YKNEKFTGRVYRIAPTINAVTRSFNVEIEVPNRNEMLKPGMFVRVSLNLGEVETFVVP
                     ASTVLMQEGTNRRYVMIAGDGVARRVDVVIGKRFDDNLEIISETLKEGDLLITEGQSR
                     LSDGDKIEVVQ"
     gene            complement(1263..2603)
                     /locus_tag="PLG42_08665"
     CDS             complement(1263..2603)
                     /locus_tag="PLG42_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOU31318.1"
                     /translation="MKTVKYFFALICALLPSLALLSQSSSTMELSLTLKDAQDYAVKK
                     NKMVAAARMDVIASKDALWETISNGLPQINATGSFTDNLKLVTTLLPGEFFGQPGVKI
                     PVRFGSQFTSGATFQASTLIFNAPYLIGVETAKLAQKLSEHNLTRTELDTKETVSMTY
                     FLILVSERSLQIVERNLANLNETLKATQAMLAAGMAEQTDVDQMMANVKSVENTKSQL
                     ERTIELNYNMLRFQLGVPADTKIVLKETLETIESQINVEALLSKEFDYRQNVNYILSE
                     DQEKLSLLALKSQKAAILPTLSGFYNYGVNGMGDKISDLRWFKNSMTGLQLSFPIFAS
                     GHRYAGIRKAQINLEKARTTKEMVTEQLLIQEKQLRYNLINANMQYKTQKENVELSKR
                     VYESMENKYRHGMASSIELTQANSLYLQAENNCISALMSLLQTKIALDKLLNNL"
     gene            complement(2618..3223)
                     /locus_tag="PLG42_08670"
     CDS             complement(2618..3223)
                     /locus_tag="PLG42_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU31319.1"
                     /translation="MDNRTKILEGAAELFRRYGIRSVTMDMIASHVGVSKRTIYETFS
                     DKNELLAGVLSLMASKQKNLIEKILSESGNIIAAIFRLMEVHIEHIQSMSPGFFDDMK
                     KFHQELAGRKDLKCEIPDYRNNRQAIERGVKEKLFRKDINIDIVNRTLYGMMFSVMNN
                     DLYPFEQFSRRDVLKNTIVIYLKGIATPEGLEIIQKLEKNL"
     gene            complement(3351..>4400)
                     /locus_tag="PLG42_08675"
     CDS             complement(3351..>4400)
                     /locus_tag="PLG42_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU31320.1"
                     /translation="AESPGGTGPFTFTWHRWDVAAKDFSIFIKTDANVSSSSLTGLDE
                     GGYRVRITDGGGYDTSLVAWVHLDRPYAEAKLQNFTCDYVALSGKAAIDTFYYYDPVN
                     GLPVRLKNAVSFLWSSTPESAIPYPSLELNPVTYSPPLEDVVYKLQVADSFQCTSESS
                     FPYTSIHVKADFEVSPLQGEAPLEVTFTDKSIRALHYEWKFGDDSVSYLKNPPPHIYY
                     KPGEYYATLIVTSEHLCIDSIRSEKITVDPSELSIPNVFTPDGDGINDYFIVKGKSLR
                     SLSIQIYSRSGMMVYSFEGEGERLSEWQGWDGCINRSSIKAAPGIYFYIVRARGWDDI
                     IYNGKEYRGFFYLYR"
BASE COUNT         1130 a          973 c          903 g         1394 t
ORIGIN      
        1 tgcacttgta cccggataag tggtaagtac agtgatatat ggcaattcaa tttctggata
       61 aagatccaca ggaagctgta caagagagta cagacctatt actatcattg ccacaaaaac
      121 caggatagtt gtaaccggcc tttttaccga tgaactgtat atgctcattt tgtttcttct
      181 ttaatttaat tattgaacaa cttcaatttt atcgccgtct gaaagtctcg actggccttc
      241 agtaatcagc aaatcacctt ctttaagtgt ttcggatatt atttcaagat tgtcatcaaa
      301 tctttttccg ataaccacat ccactcttct tgcaacgccg tcacctgcaa tcattacata
      361 ccttctgttg gtaccctcct gcatcaatac tgtagaggca ggtaccacaa atgtctcaac
      421 ctcaccgagg ttcagtgata ctctgacaaa catgccgggt ttgagcattt cgtttctgtt
      481 ggggacttca atctcgacat tgaatgacct cgttacagcg tttatggtag gagcaatcct
      541 gtaaaccctg ccggtgaatt tttcattctt atacacatct gcagtgataa cagcttccat
      601 tcctttttta ataagcggat agtactgttc tgaaaggctt actataactt taaggggatt
      661 aatctgcatg agtgtcacaa cagcaggttt accgctcatt gatggggctc cggagtacat
      721 ctcgccatct tcataatatt tcccagtgac gatcccctgg aagggtgctt taagcagagt
      781 attctgttca agaaattcga cattggtttt tgtcacctcg tactgggttt taagctggtc
      841 gtactgctgc tgttttgcgc tgcccgcctt cagcaaagtg tcgagtctcc agagatcttt
      901 ctcaagactg ctgagctgga gcttcaactg ataaagctgg gtacggtcca ttaggaaaag
      961 attgtcgccc ttattcaccc tgtcgcctgc ttccacatat atcttttcaa ttcttccggg
     1021 tgaagaaggt gctacattaa cttcctcgaa agcctgtatg gtagcagaat aatctatcgt
     1081 ctttgcaatt tttgttctgg taagcggtgt tacctttact ggtgctgctg ttttgctttc
     1141 tcccgttgtt tctccgggag ttgaggtaca tgatactaat atcactcctg aaatcactac
     1201 agcgattaat gttccaaaac gtttcattat attacggttt aaaggttata caaaatatta
     1261 ttttacaagt tgttaagaag tttatcaagt gctattttgg tttgtaaaag gctcataaga
     1321 gccgagatgc aattattttc ggcctgcagg taaaggctat ttgcctgggt tagttcaata
     1381 cttgaagcca tgccatgcct gtatttgttt tccatacttt cataaaccct tttcgaaagc
     1441 tcaacattct ctttctgagt tttatactgc atgttggcat tgataaggtt atatctgagc
     1501 tgtttttcct ggataaggag ctgctctgtc accatctctt ttgttgtgcg ggctttctca
     1561 agatttatct gcgccttcct gattcccgca tatctgtgtc cgcttgcaaa tatcgggaac
     1621 gagagttgaa gacctgtcat cgagtttttg aaccatctca ggtcacttat tttatctccc
     1681 attccgttaa caccataatt atagaagcct gaaagtgtag gaagaatcgc tgctttctgg
     1741 cttttaagag ccagaagcga aagtttctcc tgatcttctg aaagtatata gttcacattc
     1801 tgcctgtaat caaactcctt cgacagaagt gcttcgacat taatctgtga ttcaatcgtt
     1861 tctagagtct ccttaagtac tatttttgta tcggcaggaa ctccaagctg gaaacggagc
     1921 atgttatagt ttagttctat tgttcgttca agctggcttt ttgtgttttc aacactcttc
     1981 acatttgcca tcatctgatc cacgtctgtc tgctctgcca tacccgcggc aagcattgcc
     2041 tgcgtggctt tgagtgtctc attgagattt gccagattac gctcaacaat ctgcagagag
     2101 cgttccgaaa caagtatcag aaaatatgtc atgctaactg tttccttggt atcaagctct
     2161 gttcgagtca ggttatgttc gctaagtttc tgggcaagtt tggcggtttc tacaccaatc
     2221 agatatggtg cgttaaaaat aagggtagaa gcctgaaaag tggctcctga ggtgaactgc
     2281 gaaccgaacc tcacaggtat cttcacgcca ggctgcccga aaaactcgcc cggcaacagt
     2341 gtggtcacca gtttaagatt gtcggtaaat gaacctgtag catttatctg cggaagacca
     2401 ttagaaatgg tttcccacaa agcatccttc gaagcaatta catccatcct ggcagcagca
     2461 accatcttat tctttttaac agcataatcc tgtgcatcct tgagtgtcag actcaactcc
     2521 atcgtgctgc tgctctggga taaaagtgcc agagacggca ataaagcgca aatcaatgca
     2581 aaaaaatatt tcacggtttt cataatgtta tagagtttta aagatttttt tcaagttttt
     2641 gaattatttc caatccttca ggcgttgcta tacctttcag gtatattacg atagtattct
     2701 tcagtacatc tcgcctgctg aactgttcaa agggataaag gtcattgttc ataacactga
     2761 acatcatccc gtaaagtgtc ctgttcacaa tatctatatt aatgtctttt ctgaagagtt
     2821 tctctttaac tcccctttca attgcctgcc tgttgtttct gtagtcgggt atttcacact
     2881 tgaggtcttt tctgccggca agttcctgat ggaacttctt catgtcgtca aagaaccccg
     2941 gactcattga ctggatatgt tcgatgtgaa cttccatcag tctgaaaatg gctgcaataa
     3001 tatttcccga ctcactgagt attttttcaa tgaggttctt ctgtttgctc gccatcaaac
     3061 tcagaactcc ggcaagaagt tcattcttgt ccgaaaaggt ttcataaatt gttcttttcg
     3121 atactccgac atgtgaggcg atcatatcca tggttactga tcttattccg tacctgcgga
     3181 acaattcagc tgccccttcg agtatttttg tcctgttgtc catagcttta aaaaactccg
     3241 caaaaatgag aaaacttttt tagtttaaaa agttttttct gttaataaac agttaaaatc
     3301 agtaaaaggt tcaaaattac cggtgaatgg cgaagaaata tttggtggtt ttacctgtag
     3361 aggtagaaga aacctctgta ttctttgccg ttatagatga tatcgtccca gcctctggct
     3421 cgtacgatgt agaaatatat ccccggtgca gccttgattg aggagcggtt gatacagccg
     3481 tcccatccct gccattcgga aagccgttca ccttcgccct cgaaactgta aaccatcatt
     3541 ccgctgcggc tgtatatctg aatgctgaga cttctgagtg attttccctt cacaatgaaa
     3601 tagtcgttta taccgtcgcc gtcgggtgtg aacacattgg gtatggaaag ttcagacggg
     3661 tcgactgtta ttttttcact tctgattgaa tcgatgcaaa ggtgttcact tgtcacaata
     3721 agagttgcat aatattctcc gggtttgtaa tagatatgcg gaggcgggtt tttaagatat
     3781 gatactgagt cgtcgccgaa tttccactca taatgcaagg ctcttattga cttatcggta
     3841 aaagttactt ccagcggtgc ctcgccctgc agagggctca cttcaaaatc ggcttttaca
     3901 tgtattgagg tatagggaaa tgatgattcg gaagtgcact gaaagctgtc ggcaacctgg
     3961 agtttataaa ctacatcctc gagaggcggg ctgtatgtta caggattaag ttcaagcgac
     4021 gggtaaggta tggcagactc gggagtggaa gaccacagaa acgagactgc gtttttcagc
     4081 ctcacaggca aaccgtttac agggtcataa taataaaagg tatctatggc agctttaccc
     4141 gacagtgcca cgtagtcgca ggtgaagttc tgaagttttg cctcggcata cggcctgtcg
     4201 agatgaaccc atgccacaag cgatgtgtcg tatcctccac cgtcagttat ccttactctg
     4261 taaccacctt catccagacc ggtcagagat gaacttgaaa catttgcatc tgtcttaatg
     4321 aatatgctga aatctttagc cgccacatcc caccggtgcc atgtaaaagt gaacgggccc
     4381 gtccctcccg gactttcagc
//
LOCUS       DAOSNB010000217         4337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20308_length_4337_cov_5.385567, whole genome shotgun sequence.
ACCESSION   DAOSNB010000217 DAOSNB010000000
VERSION     DAOSNB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20308_length_4337_cov_5.385567"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLG42_08680"
     CDS             <1..196
                     /locus_tag="PLG42_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31321.1"
                     /translation="LTKDESEQAIYQSVVAALNLFYKSGLAPAEVIDLIPVKPIGEHE
                     EQIAEFYRTKLLGLFEKIKP"
     gene            complement(204..617)
                     /locus_tag="PLG42_08685"
     CDS             complement(204..617)
                     /locus_tag="PLG42_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31322.1"
                     /translation="MKTEKIKLPENHRRSLTSALMTVEQMLFDLEASLKYQQKGCCYE
                     IENDLADDVIKNDLEIIREARSRLCKLSEKYDSGKYIQNLSKIVNARKTRIWEVLMDM
                     KPRKQKGFGEFPKELAKEYERDLDELLSVTEKIIY"
     gene            complement(670..2388)
                     /locus_tag="PLG42_08690"
     CDS             complement(670..2388)
                     /locus_tag="PLG42_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOU31323.1"
                     /translation="MSKSDIINSDIEILREIARRLNKTGIINSLDELSGKISLKLFYL
                     VVVGLFKRGKSSVINALIGEELAPVAVTPVTSVITFFQYAPETRAEVIFRGGRRYTIN
                     PADVTYYISEENNPKNKKEVDCVRIFTKSPLLENLILVDTPGLGSLFSHNTATTIEFL
                     PRIDAALFVMSADLPISKADEELLKQIKTSVPYVLFVLNKSDLLGTDDLKKMISYNIK
                     MLKEIFGEDSAPELIAVSARKFFSNNDNYEPDHGNIKALREKISSRILIGKDEILTSR
                     SINLLLTYSNHLQTLLKVKSDTLQMPLNELEKKLEAMQNSIDYLASGKVDFDAVVKSR
                     IEQLINKVTEVTENKRRDLEAYVNAILINNSTGTWKKIKESDADEFARKLSNEIIKEY
                     EELKTSLEESVKEEFSNILLQYSTLSQSFIKEIIRQMNEVLGVNIEGIIAAFDLDVYT
                     SFYFKTETNYSVPSIRKNIFYKILPEYFVRKMVLKQIYYNSIELINPNAGKMRSDIDY
                     KISESYRKFKYHFDQKLFDLLQSLKKMIEESIENKLSINENIEFSIKIINAEFDKIEA
                     IKKHYT"
     regulatory      complement(2476..2539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2638..3540
                     /locus_tag="PLG42_08695"
     CDS             2638..3540
                     /locus_tag="PLG42_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOU31324.1"
                     /translation="MLYYFDVLNYYTWVSDKITDQIPLINVWIVIFAIVVLLCFLTGE
                     ITGNYSQTDKLWSLMPIIYGWVTVACFPFSARLWLMAIMVTIWGARLTYNFYRKGGYS
                     IVPWKGEEDYRWKYLRELPVLKGRIKFALFNLFFISFYQHFLILLFSSPFLVVAKSGK
                     ADISFADFVIAAFMLLFIVIETIADNQQYSFQRIKRGLEINTNKYIISLDKGFLTEGL
                     WRYARHPNYAAEQAIWICFYFFSVVATGQWINWSLSGAILLVLLFQGSTLMTEKISSS
                     KYPDYENYKKNVPAFIPYLFKSSK"
     gene            complement(3578..3793)
                     /locus_tag="PLG42_08700"
     CDS             complement(3578..3793)
                     /locus_tag="PLG42_08700"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HOU31325.1"
                     /translation="MEIISESSITCPVCGHQATETMPTDSCQFFYECKKCHTVLKPKP
                     SDCCVYCSYGTVPCPPIQEEKNKNTGI"
BASE COUNT         1250 a          721 c          826 g         1540 t
ORIGIN      
        1 gctgacgaag gatgaaagtg aacaggcaat ataccagagt gttgttgcag cgctgaacct
       61 gttttacaaa tcaggtctcg cacctgcgga agtaattgac ctgataccag tcaaacctat
      121 cggtgagcat gaagaacaga ttgcagagtt ctacagaaca aaacttctgg gattatttga
      181 aaagataaaa ccctgaaagg gcatcagtaa attatttttt cggtaacaga aagaagttca
      241 tccagatctc tttcatattc tttggcgagt tcttttggaa actcgccaaa gcccttctgt
      301 tttctgggtt tcatatccat caggacttcc catattcgag tttttctggc atttactatt
      361 ttactcaggt tttgaatata ttttcctgag tcatattttt ctgataattt acatagacgg
      421 ctgcgggctt cgcggataat ttcaaggtcg tttttgataa catcatcagc caggtcattc
      481 tcaatctcgt aacaacaacc tttttgctgg tattttaatg atgcttccag gtcgaaaagc
      541 atctgttcaa cggtcatcag cgctgatgta agagaacgac gatggttttc ggggagtttt
      601 attttttctg ttttcataat atttcagtta ataatagttc aaaaaattgc agccatgata
      661 tgaaaaaagt tatgtgtaat gtttcttaat agcttctatt ttatcgaatt cagcatttat
      721 aattttaatt gagaattcta tattttcgtt tatagataat ttattttcta tactttcttc
      781 aatcattttt ttcagacttt gaagcaagtc aaaaagcttc tggtcgaaat gatatttgaa
      841 ttttctatat gattcgctga ttttgtaatc aatatcactg cgcatcttcc ctgcattggg
      901 attgatcagc tctatggagt tatagtaaat ttgtttaagg accatcttcc tgacaaaata
      961 ttcaggcaga attttataga atatattttt tcttatagaa gggaccgagt aatttgtttc
     1021 tgttttgaaa taaaatgatg tatatacgtc cagatcgaat gcggcaataa tcccttcaat
     1081 attaactcca agcacctcat tcatctgtcg tataatttcc ttaataaacg actgtgataa
     1141 tgtactgtac tgaaggagaa tattgctgaa ttcttcttta accgattctt caagactggt
     1201 tttcagttct tcatattctt taattatttc atttgataat tttctggcaa actcatcagc
     1261 gtctgattcc tttattttct tccaagttcc ggtgctatta ttaataagta ttgcattcac
     1321 atatgcttcg aggtctcttc ttttgttttc tgtaacttct gtcactttat ttataagttg
     1381 ttctatacga cttttaacaa ctgcatcaaa atcaactttg cctgaagcca ggtaatcaat
     1441 tgaattctgc atcgcctcca gttttttctc aagttcatta agaggcatct gaagggtgtc
     1501 tgactttact ttcagcaagg tttgcagatg gttggaatag gttagcagca ggtttatact
     1561 tcgtgaagta agaatttcat cttttccgat aaggattctg ctgctgattt tttctctaag
     1621 cgctttgatg tttccatgat ctggttcata attatcattg ttactgaaaa attttcttgc
     1681 agaaacagct ataagttctg gagccgaatc ttctccgaaa atttccttaa gcattttaat
     1741 attataactg atcatttttt tgagatcatc agttccaaga aggtctgatt tattaagaac
     1801 aaagagtaca taaggtacag aagttttaat ttgctttaga agttcttcat cggccttgct
     1861 gattggaagg tcggcactca taacaaagag cgctgcatca attctgggaa gaaattcgat
     1921 tgtggtggca gtgttatggc tgaaaagtga acccagtcct ggagtgtcga ccagaatcaa
     1981 attttcaaga aggggggatt ttgtaaaaat tctgacacaa tcaacctctt ttttattttt
     2041 tgggttattt tcttctgata tgtaataggt tacatctgcc ggattaatgg tgtaccttct
     2101 tcctcctctg aaaataactt cagccctggt ttcaggtgca tattggaaga atgtgattac
     2161 tgaggttaca ggggttactg ccacaggtgc aagttcttct ccgattaatg catttattac
     2221 ggatgatttg cctctcttga ataaccctac aacaaccaga tagaataatt tcaaagagat
     2281 ttttcctgag agttcatcga gggaatttat aatacctgtc ttatttaatc ttcttgcaat
     2341 ttctcgtaga atctcaatgt cggaatttat tatatctgat tttgacatgg ctttatgact
     2401 gaaaaatgaa ataactcctg ttttaaataa accatttaca aattatctta cataagattt
     2461 aaaatgacct aatgtaaatg tgcaggagtt atcagtcaat ttatgacggt taagggcgaa
     2521 ctccatcgcc gctgcaaaga taattttttt aagtgttctt aaaaattgtc agaaaaggaa
     2581 atattcattt gattagattt gctttttaat tacctgttaa attaaatctg gataagtatg
     2641 ctatattatt ttgacgtgct gaattattat acatgggttt ctgataaaat aacggatcag
     2701 attcctctta taaatgtgtg gatagtaatt tttgcaatag ttgttctatt gtgttttctt
     2761 actggagaaa taactggaaa ttacagtcag accgacaaac tctggagtct gatgccgatt
     2821 atttacgggt gggtaacagt tgcatgtttt ccgttttcgg caaggctttg gctaatggcg
     2881 ataatggtaa cgatatgggg ggcaagacta acatataact tttaccgtaa aggcggttat
     2941 agtattgttc catggaaagg cgaggaagat tacaggtgga aatatctcag ggaattacca
     3001 gttttaaaag gccgaataaa gttcgcgtta ttcaatttgt ttttcatatc attttatcag
     3061 cattttctga tattgctatt ttcttcacct tttttagtgg tagctaaaag tggaaaggca
     3121 gacatctcat ttgctgactt tgttatagca gcctttatgt tgttatttat tgttattgaa
     3181 acaattgccg acaatcagca atacagtttt cagagaatta agagaggtct ggaaataaat
     3241 acaaacaaat atattatttc acttgataaa ggttttttaa cagaagggtt gtggcgttat
     3301 gcacgtcatc ccaattatgc cgcagagcag gcgatatgga tttgttttta cttttttagc
     3361 gttgttgcaa ctggccaatg gattaactgg tcactttccg gtgccattct tcttgtacta
     3421 ttatttcagg gaagtacctt gatgacagaa aaaattagca gttcaaaata tcctgattat
     3481 gaaaattata aaaaaaatgt tcctgcattt attccgtatc tctttaaaag ttctaaatga
     3541 aaattattaa tgttggtttg attttctaac acatacttta gattccagta tttttgttct
     3601 tttcttcctg tatcggaggg cagggcacag tcccataaga gcagtaaacg cagcagtcgc
     3661 tcggtttggg ttttagcact gtatggcatt ttttgcattc ataaaagaac tgacatgaat
     3721 cggtgggcat ggtttcagtt gcctggtgcc cgcacacagg acaggtgatt gaagattcgg
     3781 aaataatttc catttggtgc attttaaagt ttcggcttgg cagcaataaa tcttcaattt
     3841 aagcgctttt acaagctctt cttcatgaaa atatccgaaa tgcaagtaat attctttacc
     3901 gtccttatca aataataact gggtggggat tgcttctatg ttgtattgtt gtgcaaaggg
     3961 agcccctgtt attcctgttt aaatggccga ctttgaaagg aagaccaaca ctgctgccga
     4021 attaaggaaa ttcttttcaa attgttgatg tcgggtatat ctcgtaaaat gtttatctct
     4081 ggtgtgcgtc agaaggactt gcatcaatta cccttggaca ggtagataaa cctatggttt
     4141 atgaagtcct gaaacttaaa tcgtctcggc agttagtttt tcgcaaactg tgggttatac
     4201 tgaacaatag gtaagggtaa gatcccggtg atcagctaag tttaaaagaa gaggttaaat
     4261 aaataaccgc tgattacggc ggtcaggaat attgttgtaa ccattgcagc aactagtttt
     4321 ggtttaaaga tccctgc
//
LOCUS       DAOSNB010000218         4331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20348_length_4331_cov_4.350094, whole genome shotgun sequence.
ACCESSION   DAOSNB010000218 DAOSNB010000000
VERSION     DAOSNB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20348_length_4331_cov_4.350094"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PLG42_08705"
     CDS             complement(<1..992)
                     /locus_tag="PLG42_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU31326.1"
                     /translation="MNKINRRNFILTSVTGAAGIFFYNPARANPVRETGENLLTRVAL
                     TAGDNRADMTFRALKPFAKQIKQAIGNRRVVLKPNNVSTSIQLSATHADTLEGILEFL
                     KSIGKLNNVVIAESAADGPAMDGFSNFNYLRLANKYPVKFIDLDTAGFETLYVFDERD
                     FKPNPVRISKVLLDPGSYIVSVARMKTHDRVVVTLSLKNIVVGAPVKDPGFTFGKNRK
                     EGTRSDKSIVHGGGYRGIHYNLYDLASRLHPHLAVIDGYEGMEGNGPTGGTPVDHRVC
                     VVSTDWLAADRVAVELMGVDFNNVAYLNYCAQAGLGVADLGKIEIIGESISNHI"
     gene            1217..2113
                     /locus_tag="PLG42_08710"
     CDS             1217..2113
                     /locus_tag="PLG42_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOU31327.1"
                     /translation="MIYARKFNFSETQRFIRVFFVLFLCVPLSMMAQLKIYVNTDLEG
                     ISGVFKFDQTREKDTPLNIQACEYFMGDVAAVVRGLIDGGATEVIVLDGHGTQAVIPH
                     LMEPGAKYITGKPRPGAGNLSLLDSSFSAMVMIGFHAMMGTPDGVLNHTQSSRSENRY
                     WYNGIESGELAQNALIAGYYGVPVIMVTGDEATCREARKFFGENIVTVATKKGLGRES
                     AVLYPFEETRKALYEGAKNAISKIPLCKPYRIKEPIQVKMQFLDLDPKLPKPELVTRE
                     GIAKNGKHILDFQILAEYRNRE"
     gene            2163..2771
                     /locus_tag="PLG42_08715"
     CDS             2163..2771
                     /locus_tag="PLG42_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOU31328.1"
                     /translation="MNKRTFIKTTLVGIAAIFVPRRSKALEYYPRPSDKKWAVLYSTW
                     CGSARDAGVWISEGMDGIANVFDVRENPDLSRYDYVIVGGAIRAGRVSQEMQDYLSRN
                     REILKTRLRGYFAVCGNMMQPVTPEVTARLIDKHLAVICGVTGIPSKVFLGRITFGLM
                     EPDVREQMQKMPGIKEYDNLKRDECMAFGKEVLAATSKHKPT"
     gene            complement(2885..4234)
                     /locus_tag="PLG42_08720"
     CDS             complement(2885..4234)
                     /locus_tag="PLG42_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU31329.1"
                     /translation="MRNIVIIFMFVFFAGCISNRETDYYSGKQVEVLKEVQNRSIDSS
                     TIRSIIAPFKPDRIITVGGAGADMKGFTSEAIQTAIDAIRVEGGRGTVILLQGEFEVI
                     APVRLYSNITLKGSGEKTILKKCKGYSSPFAVDADYGELQVTVQDASGFKPGMGVAIF
                     DEDQRYGWDLTTAKITAIKGDTLFIDEFLVRDYRSDKKGTVSNACSVISAVEAENIKI
                     ADLVVDGSAGSNDMIDGCRAGGIYLHKVRNAVIENVTVKNFNSDGISWQITEHVTVRK
                     CEVYGCTNSGLHPGTGSPYTVIEGNRSHDNGGYGLFVCWRVRNGIVRDNIFYRNGRNG
                     ISTGHKDTDMLFSGNHIYENGSDGISLRGETDLNAPHRSVFRNNIIENNGTREKGYGI
                     GIYSKAEDVVIENNVIRNTGNNKQIAAIYLSANSLPVKVENNEFSGHSAGDIISDQK"
BASE COUNT         1303 a          931 c          811 g         1286 t
ORIGIN      
        1 tttatgtggt tacttatgct ttcacctatt atttctattt tcccgaggtc agcaactcca
       61 aggccagcct gtgcacaata gttcaggtac gccacattgt taaaatcgac acccatcagc
      121 tccacggcaa ccctgtcggc agcaagccag tcggtactga caacacaaac cctgtggtca
      181 accggggtcc cgcctgttgg accattaccc tccattcctt cataaccatc aataactgcc
      241 aggtgaggat gaaggcgtga agcaagatca taaagattat aatggatacc cctgtaacct
      301 ccaccatgta caattgattt atctgacctt gttccttctt ttctgttttt cccgaaagtg
      361 aatcccggat ctttgacagg agctcccaca acaatgtttt taagcgaaag agtaactacc
      421 acacgatcat gtgttttcat tctggctacc gatacaatgt aagaaccagg atccagcaat
      481 acttttgaga tccttacagg gttaggtttg aaatccctct catcaaaaac atataaagtt
      541 tcaaaccctg cagtatcaag atcgataaat tttacaggat atttattagc cagcctcaga
      601 tagttgaaat tactgaaacc gtccatagca ggaccatcag cagctgactc tgctataaca
      661 acattgttaa gcttcccaat tgacttcagg aattcaagta taccctcaag tgtatcagca
      721 tgtgttgcac tcaactggat ggaagtagat acattatttg gtttaagaac cacacggcga
      781 ttccctattg cctgctttat ctgctttgca aatggtttaa gtgcccggaa agtcatatca
      841 gccctgttat cacccgcagt aagagcaacc cgtgtaagga ggttttctcc agtttctctg
      901 accggattgg ctctggccgg attgtaaaag aaaatcccgg ctgctccggt aactgatgta
      961 agaataaaat tgcgcctgtt gattttattc ataataatct tttccgattt agtaaaatta
     1021 caaaaaagat ttaccattaa ttttttatct cctgttttaa aaaataatta gcaggaaaaa
     1081 tttatcattt cttatgtcag gcagaaaagc cacatgatta gttatttata actctattcc
     1141 aaatctggat ttatgcaatt acaataagaa taaaactttt gtatttttat aacaattaca
     1201 aaaaagaagt gtataaatga tatatgcaag aaaatttaac ttttctgaaa cacaaaggtt
     1261 tattagagtg ttttttgttc tgtttctgtg tgttccattg tccatgatgg cacaactcaa
     1321 gatttacgtc aatacagatc ttgagggtat aagtggggtt tttaagtttg atcagacacg
     1381 cgagaaggat actccactga atattcaggc atgtgaatat tttatgggag atgttgcagc
     1441 tgttgtacgc ggtcttatag atggtggcgc aaccgaagtt attgttcttg atggtcacgg
     1501 tacccaggca gtgatcccac atcttatgga acccggagca aaatatatca caggaaaacc
     1561 acggcctggt gccggcaacc tgtcgctact tgacagttcc ttttctgcga tggtaatgat
     1621 tggttttcat gcaatgatgg gcacccccga cggagtgctg aatcacacgc aaagttcacg
     1681 ttctgaaaac agatactggt ataatggtat tgaatcaggc gaactggcac aaaatgccct
     1741 tatagcaggt tactacggcg ttcctgttat aatggtaacc ggcgatgaag caacatgccg
     1801 ggaagcaaga aaattcttcg gagaaaatat tgtcacagtt gcaacaaaaa aaggcctggg
     1861 aagagaatca gctgttcttt atccctttga agaaactaga aaagccctct atgaaggagc
     1921 aaaaaatgca ataagtaaga ttcctttatg caaaccatat cggattaaag aacctattca
     1981 ggtcaaaatg caatttctgg acctcgaccc aaaactgccc aaacctgaac ttgtaacccg
     2041 tgaaggcatt gcaaaaaacg gaaagcatat acttgatttt cagatattag ctgaatacag
     2101 aaaccgggaa tgaaaagcat ggaggaaata taaaatgtta agtataaata aactgttaaa
     2161 atatgaataa gagaactttt atcaagacaa cgttggtggg aattgcggca atttttgttc
     2221 cccgcagaag caaagccctt gaatattacc cccgaccttc tgataagaaa tgggcagttc
     2281 tttacagtac ctggtgcggt tcagcacgtg acgcaggtgt atggatttcg gagggaatgg
     2341 atggcatcgc aaatgtattt gatgtccgtg aaaatccgga tctttccagg tatgattatg
     2401 ttatagttgg tggagctata agagcaggca gggtttcaca ggaaatgcag gactacctgt
     2461 ccagaaaccg ggaaattcta aagacaaggc ttagaggata tttcgcagta tgcggaaaca
     2521 tgatgcagcc agttaccccc gaagtcactg caaggctaat agacaaacac cttgctgtaa
     2581 tatgcggtgt aacaggaatc ccctcaaaag tcttcctcgg ccgaatcaca ttcggtctta
     2641 tggaacctga tgtgcgggaa cagatgcaga aaatgccagg tataaaggaa tacgacaacc
     2701 ttaagagaga tgaatgcatg gccttcggaa aagaagttct ggctgcaacc agcaaacata
     2761 agccgactta attaaacatc catgtcaata tcaatctttt tttctttttt ttcggatatt
     2821 ccgaaattta agatgaaaaa tataatatcg ctcgccggca gaaatcaatt aaatatttta
     2881 ctgattattt ctgatcacta atgatatctc ctgcagaatg gcctgaaaat tcattgtttt
     2941 ccacttttac gggaaggctg ttggcagaaa gataaatagc ggcaatttgt ttattattac
     3001 ccgtgttgcg tataacatta ttctctatta caacatcttc tgctttgctg taaataccaa
     3061 taccgtagcc tttttcccgt gttccgttat tctcaattat attgtttctg aatacacttc
     3121 tgtgtggcgc attaaggtcc gtttcaccgc gaaggcttat tccgtcagac ccgttttcat
     3181 atatgtgatt gcctgaaaaa agcatgtctg tatccttatg tccggttgaa ataccattcc
     3241 tgccgtttct ataaaatatg ttgtccctta ctataccgtt tcttacccgc cagcatacaa
     3301 aaagtccgta acctccgtta tcatgactcc tgtttccttc gataaccgta taaggggaac
     3361 ctgttccggg atggagaccg ctgttggtac atccatatac ctcacatttc cttacagtta
     3421 catgctcggt aatctgccag ctgataccgt cgctattgaa atttttcact gtcacatttt
     3481 cgataacagc attacggact ttatgcagat atatgccacc cgcacggcat ccatctatca
     3541 tatcattact gcctgcactt ccgtcaacaa ccaggtctgc aatctttata ttttcagcct
     3601 caacagctga tatcaccgaa catgcattcg atacagtacc ttttttgtca gaacggtaat
     3661 ctcttactag aaattcatca ataaaaagtg tatctccctt tatagctgta attttagcag
     3721 tagtaagatc ccaaccgtac ctctggtctt catcaaatat tgctacaccc attcctggtt
     3781 taaatcctga agcatcctgt acagttacct gaagttcccc ataatcagca tccacagcaa
     3841 aaggagagct gtagccttta cattttttca gaatggtctt ttcacccgaa ccctttaatg
     3901 ttatattgct gtagagccgc acgggagcaa taacttcaaa ctcgccttgt aatagtatga
     3961 ctgttcccct gccaccttct actcttattg catcaatagc tgtctgaatt gcctcggaag
     4021 taaatccttt catatcagca ccagcaccac ccactgtaat aatccggtca ggcttaaaag
     4081 gagctattat acttcttatt gtagatgaat ctatgcttct gttctgaact tctttaagta
     4141 cttcaacctg tttccctgaa taataatcag tttcacggtt gcttatgcag ccagcaaaaa
     4201 acacaaacat gaaaataata actatgttcc tcataatcaa atcttttaat ttgttaacaa
     4261 tctgtaatat ataaaaacag atacttaaaa aacctgtttt aatcctgttg aatgtcgtta
     4321 cattcgggcc t
//
LOCUS       DAOSNB010000219         4315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20445_length_4315_cov_3.909859, whole genome shotgun sequence.
ACCESSION   DAOSNB010000219 DAOSNB010000000
VERSION     DAOSNB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20445_length_4315_cov_3.909859"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="PLG42_08725"
     CDS             <1..2179
                     /locus_tag="PLG42_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU31330.1"
                     /translation="RSKSFITKSGVSMLGISDAAESIGFRTRGYRLTWEQLRDDVPLP
                     CIVHWNQRHFVVVYKIKKHRAQGTGHRTRSAEKKSEVRQFSPFSPGRRAGDEVFVADP
                     AAGLLKYNEKDFCKCWFSTKTDGVDEGTALLLEPTPEFYKQEDEEKSKLSFLYLLNYI
                     RPYTKYIFQLMLGMLTASIISLIFPFLTQSLVDTGIGNSNISFVVMVLVAQLILTLSQ
                     TANGLLRNWISLHVTSRVSISLISDFLIKLMKLPISFFDVKLVGDIMQRIGDHNRIRT
                     FLTDSLISIIFAAITLVMYTIIMATYNLGILGIFMLGSTMYIGWVVLFLKRRRELDYK
                     RFQQSAANQSNIVQLVTGMQEIKLNSCEKQKRWEWERIQIKLFKVSLKSMMLNQNQQL
                     GAVLINQTKDIFISFLSAKAVIEGQMTLGMMMAIQYIIGQLNAPIQQFIGFTQAAQDA
                     RISLERLGEIHNREDEEKPEDDKIREIPADKDIEIKNLLFQYEGPHSEKVLNNVSLTI
                     PAKKITAIVGISGSGKTTLLKLMLGFYEPVKGEISLNGTPLKKFSQAEWRKRCGVVMQ
                     EGYIFADSISGNIGLADDMPDKEKTDRAAKTANIKEYIDSLPLGYNTRIGNDGQGLST
                     GQKQRMLIARAIYKEPDFIFFDEATNALDATNERTIMENLGTFFKGKTVVIVAHRLST
                     VKNADQIVVLEKGEIAETGTHKELVEKKGAYYNLVKDQLELGG"
     gene            2310..3605
                     /locus_tag="PLG42_08730"
     CDS             2310..3605
                     /locus_tag="PLG42_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU31331.1"
                     /translation="MPEIDKHEIRSPELQEVMSEIPGRFLRWGLFLFFGIILAILGVT
                     WFINYPDIVTAPVTITTYNSPAPLVAKSGGKIEKLLVDNGEDLTTDQSVAIIENTAVY
                     EDLLTLVLFLDTLDHNPDWQNKVAKYIPPENLSIGEIQTSYSRFVTYFFQFREYLKQS
                     YILSKLKLLEQQIRNQQEYTAELLTQERLSEEDLKLVFRSYERDSVLYYSSLHSVTLS
                     EYERSKQVLIQKKSAHSSLRASIKNNESSALKMKESRLDLLVQLDKELHQYILDLDDS
                     YKMLTLAIDQWKEKYLVKSPVKGRITFTSYWNINQVIKAGEILATVIPDDPSRIIVRA
                     NVPVSGLGKVKTGQEANIKLSGFPYMEYGVLKGKISILSLVPAGDYYIAEIDLADGLK
                     STYGIKLDFINDMTGTADIITENSRLFFRLIKPLRSILN"
     gene            3911..4141
                     /locus_tag="PLG42_08735"
     CDS             3911..4141
                     /locus_tag="PLG42_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31332.1"
                     /translation="MKKLKKLQINHEKLMKNEELLILRGGYQGGNTYCLCNCTLIPLA
                     WWKCYSSVFEMEYDIDIKCGRDGGCSCSDYLC"
BASE COUNT         1387 a          814 c          968 g         1146 t
ORIGIN      
        1 ccgttccaaa tctttcatta caaagtctgg tgtttcgatg ctcggtatca gcgatgctgc
       61 cgaaagtatc ggctttcgta cccgcggcta ccgtcttacc tgggaacaac tccgcgatga
      121 cgtacctctg ccatgtatag tccactggaa ccagagacac ttcgtagtgg tatataaaat
      181 caagaagcac agagctcagg gcacagggca caggacaagg agcgcagaaa aaaaatccga
      241 ggtacggcag ttttctccct tctcccctgg gagaagagcc ggggatgagg tttttgttgc
      301 agatcccgcc gccggtcttc ttaaatataa cgaaaaagat ttctgcaaat gctggtttag
      361 cacaaaaacc gacggtgttg atgagggaac cgctttactg ctcgaaccga cacctgaatt
      421 ttataaacag gaggacgaag aaaaaagcaa actgagtttt ttatatctcc tgaactatat
      481 aagaccttat acaaaatata tcttccagct tatgctggga atgcttacag ccagcattat
      541 aagccttatt tttccttttc tcacccagtc gcttgtcgat accggcatag gaaacagtaa
      601 tatttcattt gttgtaatgg tgcttgtcgc ccagcttatt ctcactctga gccagactgc
      661 caacggacta ctgcgaaact ggataagcct ccacgtcaca agcagggtaa gcatatcgct
      721 aatctcagat ttccttataa agctaatgaa actcccgata tcattctttg atgtgaaact
      781 tgttggcgat ataatgcaga gaattggcga tcataaccgg atcaggactt ttctcaccga
      841 ttctcttata agcatcattt tcgctgctat tacgcttgtt atgtatacaa taataatggc
      901 aacatataac ctgggcatcc tgggtatttt tatgctcggc agtacgatgt acatcgggtg
      961 ggtggtgctt tttcttaaac gtagaagaga gctcgactat aaacgatttc agcaaagcgc
     1021 agccaaccag agcaatatcg ttcagcttgt aactggtatg caggagataa agcttaacag
     1081 ctgtgaaaag cagaaaagat gggaatggga acggatacag ataaagcttt ttaaggttag
     1141 cctgaaaagc atgatgctta accagaacca gcaactggga gccgtactga taaaccagac
     1201 caaggatatc tttatctctt tcctttcagc caaagctgta attgaaggtc agatgacact
     1261 tggcatgatg atggcaatcc agtatattat cggacagctc aatgcaccga tccagcagtt
     1321 cataggtttt acccaggctg ctcaggatgc gaggataagt ctcgaacgcc tgggagagat
     1381 acataacagg gaggatgagg aaaaacccga agatgataaa ataagagaaa tccctgcaga
     1441 taaggatatt gaaataaaaa acctgttatt tcagtacgaa ggaccgcatt ccgaaaaagt
     1501 acttaacaac gtttcattaa ctattccggc gaaaaagatc acggcaatag tcggaataag
     1561 tggcagcggc aaaacaacac tattaaagtt gatgcttggt ttctatgagc ctgtaaaagg
     1621 agagatatca ttgaacggta ctccacttaa aaagttcagc caggcagagt ggagaaaaag
     1681 atgcggtgtt gtgatgcagg aagggtatat tttcgccgat tcaatatccg gtaatattgg
     1741 tcttgctgat gatatgcccg ataaggaaaa gacagacagg gcggcaaaga ctgcaaatat
     1801 aaaggagtat atcgacagcc tgcccctggg ttacaatacc aggatcggga acgacggaca
     1861 gggcctgagt acaggacaga aacaaagaat gcttatcgcc agggctattt acaaagaacc
     1921 tgattttatt tttttcgatg aagcgacgaa tgctcttgat gcgacaaacg aaaggacgat
     1981 catggaaaac cttggtacat tctttaaagg gaaaacagtt gtcatagtag cacacaggct
     2041 cagtacggta aagaatgccg atcagatcgt tgttcttgaa aagggtgaaa tagccgaaac
     2101 cggaacacat aaggaactcg ttgaaaagaa aggagcatat tataacctgg tgaaggatca
     2161 gctggaactg ggtggctgag ggcggggagc aaggggcaaa gagcagggag caagggtctt
     2221 taggctaaga gttaggaaga tttgccctta gctctcagcc cccagctctc agtcttcaac
     2281 atttgaaata aaatagagta ttaaccataa tgcccgaaat agacaaacac gaaatacgaa
     2341 gccctgagct tcaggaggtg atgtccgaaa ttcccgggcg attcctgaga tggggattgt
     2401 ttctcttttt tggtattatc ctggcgattc tgggtgtgac atggtttatc aactatccgg
     2461 atattgttac ggcacctgta actattacaa catataactc ccctgcaccg cttgttgcaa
     2521 aatcgggagg taaaattgaa aaactgctgg ttgacaatgg ggaagatctt acaacggatc
     2581 agtctgtggc aataattgaa aatactgctg tttatgagga tttacttact cttgttttat
     2641 ttcttgacac acttgaccat aatccggact ggcagaataa agttgcgaaa tatatacctc
     2701 ctgaaaatct ttcaataggc gaaattcaga caagctattc ccggtttgta acctacttct
     2761 ttcagtttag agaatatctg aagcagtctt acattctttc aaaactaaag ttacttgaac
     2821 aacagataag aaatcaacag gaatacactg ccgagcttct tacacaggag aggctgtcgg
     2881 aagaagatct caaacttgtt ttccggagtt atgagcgtga ttctgtatta tattacagca
     2941 gccttcattc ggtaacgctt agcgaatatg aaaggtcgaa gcaggtgttg atacagaaaa
     3001 aatctgcaca cagttctctc agagcttcca taaaaaataa tgaatcatcg gctttgaaga
     3061 tgaaggaatc aagacttgat cttcttgtac agcttgacaa agaactgcat cagtatattc
     3121 tcgaccttga tgactcctat aagatgctta cgctggcaat agatcagtgg aaggagaaat
     3181 atctggtcaa aagtcccgta aaaggaagga taacctttac aagttactgg aacattaacc
     3241 aggtgataaa agctggagaa atactggcaa ccgtaatacc agatgatccg agcaggataa
     3301 ttgtcagggc aaacgtaccg gtatcaggcc tcgggaaagt aaaaaccggc caggaggcga
     3361 atatcaagct ttcgggattt ccttacatgg aatatggagt tttaaaaggg aagatcagca
     3421 tcttatctct tgtacctgcg ggggattatt acattgcgga gattgacctg gcagacggcc
     3481 tgaaatcgac atacggaatt aaactcgact ttataaatga tatgacagga acggcagata
     3541 taattacaga aaacagcaga ttatttttca gacttataaa accgctcagg agtatattga
     3601 actgaattaa tggttttaca gtttacaggt cacctggtgt atgtctatat gtggcagtaa
     3661 gccggtgcat gaaaaaaatt aaaaaatgga ttggaagaag aaagccgtaa aaacaaagta
     3721 ataaatctga aagagaaggg cagaggtttt cccctgagct cttagcttac agccctctgc
     3781 cccgaccttt cccaaacaga aaggaggtgt acacatgcga agataattct tttccgtgac
     3841 taaggtttcg gcctgtgaaa tgataaacca ggaaaactta actgaatttg taactatcaa
     3901 attgattatc atgaaaaaat taaagaaatt gcagataaat catgaaaagt taatgaaaaa
     3961 tgaggaatta ttgattttaa gaggaggata tcagggaggc aatacctatt gcctttgcaa
     4021 ttgtacttta attccgttag cttggtggaa atgctattct tcagttttcg aaatggaata
     4081 cgatatagat ataaaatgtg gcagagatgg aggttgcagt tgtagtgatt atttatgtta
     4141 attgagttct gaggtctcta aaatgataat acaaatgctt atttgatgga aaatcatcaa
     4201 ataagcatta aaaattcact ctggtatttg atctttaaat tttcacttgt ctcaatttat
     4261 gatgaaaaag ctatctgttc aatttttctt attctttatt gccggctggt gttgc
//
LOCUS       DAOSNB010000220         4308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20487_length_4308_cov_4.073595, whole genome shotgun sequence.
ACCESSION   DAOSNB010000220 DAOSNB010000000
VERSION     DAOSNB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20487_length_4308_cov_4.073595"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..334
                     /locus_tag="PLG42_08740"
     CDS             77..334
                     /locus_tag="PLG42_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31333.1"
                     /translation="MGAFMNMYINSRCEILYCWFYLDFLNINIINENELYNLYIELKN
                     LKIDTSKIYIAAENIPPFEKACDYAIFGSSLVPFEYRNKPK"
     gene            complement(479..1663)
                     /locus_tag="PLG42_08745"
     CDS             complement(479..1663)
                     /locus_tag="PLG42_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31334.1"
                     /translation="MKEKKKIRAGILGSGYAARFHFEALKRVTCADVEVVGAYSPTQS
                     RLEEFTNKRGITSYSSPAELIDDSDVVHVCTPPATHEQLAIEILAAGKHVIVEKPLTG
                     YFGDGSENFNGDTFSRREGLEQTMASLRRMLDAEKKSKGRIMYAENWVYAPAIQKERE
                     IIEKSGAQILRMLGEESHSGSHSPVYGIWKFSGGGSLIGKGCHPLAGAVYLKSVEGRS
                     RTGKPIRPVAVSARTHAVTRMPGFLNRGHLRTGYRDIEDYGIMHVVFEDGTFADIVAS
                     ELALGGVINRLEVFADNHRTLCSMTPNNAMQTYTPDEKYFKDIYIVEKTETKQGWSWV
                     SPDESWFNGYQHEMEAFYNTAAYGAPLESNSTLAADVIATIYAAYVSAAEKGSEVEVV
                     LL"
     gene            complement(1660..3771)
                     /locus_tag="PLG42_08750"
     CDS             complement(1660..3771)
                     /locus_tag="PLG42_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOU31335.1"
                     /translation="MKNYIIYFLVMTIFASCTGDSGKKLNYPVTRQDNIVDTIFGTPV
                     PDPYRWLEDDMSEETAAWVAEQNRVTFGYLEKIPYREKLKEKLTAMWNYERYSEPFIE
                     GEYTYFYKNDGLQNQSVLYRQKAGGEPEVFLDPNTFSSDGTVSLGELGFSKDGSLAAY
                     SISEGGSDWRKIIVKNALTGEIIGDTLKDIKFSGIAWQGKEGFYYSSYDRPKGSELSA
                     MTDHHKLYFHKVGTKQKEDKVVFGSDIKRRYVSASVTEDGRFLIVYASVSTTGNELYI
                     KDLTKPGSKFTTVVDNFDNDHAVIDNAGDKLYILTNLNAPNRKVVIADAKNPKPENWV
                     DFIPETENVLDAAAGAGYIFAHYMIDAISKVKQYDRQGNLVREIELPGVGAASGFGGK
                     ENYKEVYYTFTNYVTPPTIFKFDPATGKSEIYKKPAVAFDGSNYVSEQVFYNSKDGTR
                     IPMIITYRKGLKLNGKNPAMLYGYGGFNISITPSFSVPVAVWLELGGVYAVPNIRGGG
                     EYGEKWHLAGTKMNKQNVFDDFIAAAEYLIANKYTSPEYLSIRGGSNGGLLVGAVMTQ
                     RPELFRVALPAVGVLDMLRYHKFTAGAGWAYDYGTADESREMFEYLLKYSPVHNVREG
                     VEYPATLITTADHDDRVVPAHSFKFAAHLQAKYKGNNPVLIRIETRAGHGAGTPVSKS
                     IEQYADIWAFTLWNMGIKSIE"
     gene            complement(3860..>4308)
                     /locus_tag="PLG42_08755"
     CDS             complement(3860..>4308)
                     /locus_tag="PLG42_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOU31336.1"
                     /translation="TYNIYRKPVKGGEEVALTATKPGEHVDGCEYSPDGKYIYYNGSA
                     SGTMQIWRMKPDGSQKEQLTFDEYNDWFPHISPDGKWIVFLSYPVDIPPNSHPSYKKV
                     MLRIMPASGGAPKVIAWLYGGQGTINVPSWSPDSKSIAFVSNSGIK"
BASE COUNT         1169 a         1049 c          826 g         1264 t
ORIGIN      
        1 tcaaaaatat gctgaagaat tgttctcgaa tgtcatttaa aaaaatatac ctgatgaaaa
       61 attgaaaaaa aattctatgg gagcatttat gaatatgtac attaattcgc gttgtgaaat
      121 cttatattgc tggttttatc ttgacttttt gaatataaac atcatcaatg aaaatgagct
      181 ttacaattta tatattgaat taaaaaatct taaaattgat accagtaaaa tctacattgc
      241 cgctgaaaat atacctccat ttgaaaaagc ctgtgattat gccatttttg gaagctctct
      301 tgttccattt gagtatcgaa ataaacccaa ataaaagctt cctccatatc ttgctatttt
      361 ctcctgcagg aacaacatta cataaatggt tgaaatcaaa ttcattacca aaatacagtt
      421 gcttctagga ggcactgtag gaacaccagt tatccgagct ccggctgaac aaaatgtctt
      481 ataaaagcac aacctctact tcactgcctt tctcagccgc cgaaacataa gcagcataaa
      541 tagttgcaat aacatctgcc gcgagagtgc tgttgctttc aagaggagcc ccatatgcgg
      601 ctgtattata aaatgcctcc atctcatgct ggtaaccgtt gaaccacgac tcatccgggc
      661 tcacccacga ccagccctgc ttcgtctctg ttttttcgac aatataaatg tccttgaaat
      721 atttttcatc aggcgtgtat gtctgcatgg cattgttggg tgtcatactg cataatgtac
      781 ggtggttgtc ggcaaacacc tccagcctgt tgataacacc ccccagagcc agttcgctgg
      841 caacgatatc ggcaaatgtc ccgtcctcaa acacaacatg cattatgcca taatcctcaa
      901 tatcacggta tcctgtacgc agatgacctc tgttaagaaa tccaggcata cgtgttactg
      961 catgtgtccg tgctgatact gcaacgggcc ttatcggttt tccggtgcgg ctgcgaccct
     1021 ccacactctt caggtaaacg gcgccggcaa gcggatggca ccccttacca atcagtgatc
     1081 cgccacccga aaatttccat atgccgtaaa ccggtgagtg agaacccgaa tgcgactcct
     1141 ccccaagcat cctcagtatc tgtgcacccg acttttcaat tatctccctc tctttctgaa
     1201 tggctggtgc atatacccag ttttcggcat acataatcct tcctttgctc ttcttttcgg
     1261 catcaagcat cctgcgaagg cttgccatgg tctgttcaag tccttcccgc ctcgaaaaag
     1321 tatcaccgtt gaaattctca ctgccatcac cgaaataacc cgtaagaggt ttctccacaa
     1381 tcacatgttt tcctgcagca aggatttcaa ttgcaagctg ctcatgcgtt gcgggaggcg
     1441 tgcatacatg cacaacatca gaatcatcaa ttaattcagc aggagaggaa taagatgtta
     1501 tgcccctctt atttgtaaat tcttccagcc tgctttgggt gggcgaatag gccccaacaa
     1561 cctcaacatc agcacaggtt acccttttca gtgcctcaaa atgaaaacgg gcagcataac
     1621 ccgaaccgag tatacctgcc cttattttct tcttttcttt cattcgatag attttattcc
     1681 catgttccag agtgtgaatg cccatatatc ggcatactgc tcgatgcttt tgctcaccgg
     1741 agtgccggca ccatgtccgg cccttgtttc aatacgtata agtacagggt tattcccttt
     1801 gtattttgcc tgcagatggg cagcaaactt gaacgagtga gccggcacaa ccctgtcgtc
     1861 gtgatcagct gttgtgatga gtgtcgcagg atattcaacg ccttccctta cattatgaac
     1921 aggagaatat ttcagaagat actcaaacat ctccctgctc tcatcggctg tcccgtaatc
     1981 gtatgcccaa cctgcaccgg cagtgaactt atggtatcgc agcatgtcga gtactcccac
     2041 tgccggaagt gcaaccctga acaattcagg cctctgggtc attacagcac caacaagaag
     2101 ccctccgttt gaccccccac gaattgaaag gtattcgggt gaagtgtatt tattggcaat
     2161 aaggtactca gcagccgcaa taaaatcatc gaaaacgttt tgtttattca tcttcgttcc
     2221 ggcaagatgc catttctcac cgtattcgcc accaccccta atgttgggta cagcataaac
     2281 acctccaagt tcgagccaca cagccaccgg cacactgaac gaaggagtaa tacttatatt
     2341 aaaaccgcca taaccataaa gcatagcagg attctttcca ttcaatttca aacccttcct
     2401 gtaagttata atcatcggta ttctggtccc atcctttgaa ttgtaaaaaa cctgctccga
     2461 aacataattc gagccgtcga atgcaacagc cggtttcttg tatatctcgc tcttgcccgt
     2521 ggctggatca aatttaaaaa tggtgggcgg tgtaacatag ttggtaaaag tgtagtatac
     2581 ctctttataa ttttcctttc ctccaaaccc tgaagccgct ccaacgcccg gtaattcaat
     2641 ctcccttaca agattcccct gcctgtcgta ttgcttaact tttgatattg catcaatcat
     2701 gtaatgggca aaaatgtaac ctgcgccagc tgctgcatcc agaacatttt ctgtctcagg
     2761 aataaaatca acccagtttt caggtttcgg gttcttagca tcagcaatca ccactttccg
     2821 gttaggtgca ttcaggttgg taagtatata taatttgtct cctgcattat caatcactgc
     2881 atggtcgtta tcgaagttat caacaacagt ggtgaattta ctgcctggtt ttgtgagatc
     2941 tttaatatac agttcattcc cggttgtgga cacagatgca tacacaatca gaaatctacc
     3001 atcttctgta acagaagcgc ttacgtatct cctttttata tccgatccga aaacaacttt
     3061 gtcttccttc tgttttgtcc caaccttatg gaaatagagc ttatggtgat cggtcattgc
     3121 cgagagttcg cttcctttgg ggcggtcgta gcttgaataa tagaatccct ccttcccctg
     3181 ccatgcaatg cccgagaact ttatatcctt aagggtgtcg ccaattattt cacctgtaag
     3241 agcatttttg actatgattt tcctccagtc ggatccgccc tccgatattg aatatgcagc
     3301 aaggctgcca tcctttgaaa agcccagttc gccaagcgaa acagttccat cggatgaaaa
     3361 ggtattggga tcgagaaaaa cctctggttc accacccgct ttctggcggt acagtaccga
     3421 ttggttctga agaccatcat ttttataaaa atatgtgtat tctccttcaa tgaagggttc
     3481 agagtacctc tcgtaattcc acattgccgt gagtttttcc ttcagttttt cgcggtaagg
     3541 aattttttcg agatacccga atgtaaccct gttctgttct gcgacccaag ctgctgtttc
     3601 ttccgacatg tcatcctcaa gccagcggta cggatcaggc acaggagtgc caaagattgt
     3661 gtcaacaata ttatcctgac gcgttactgg gtaatttagt ttctttccgg aatcacctgt
     3721 gcaagaagca aatattgtca taaccaggaa gtatataata tagtttttca taggataaag
     3781 gttatagggt aatgattcat tattttatca gacacttact aacaaaagac aaatataata
     3841 cttcaaaaca agagtcagat tatttaatac ccgaattgct tacaaaagca atactcttac
     3901 tgtcgggtga ccaggaagga acgtttatcg tgccctgtcc gccatagagc catgctatca
     3961 ctttcggtgc accgcccgat gctggcataa tcctgagcat caccttcttg tatgaaggat
     4021 gggaattggg aggaatgtcg accggatatg acaggaaaac tatccacttt ccatcaggtg
     4081 agatatgagg aaaccagtcg ttatactcat caaatgtaag ctgttccttt tgtgaaccat
     4141 ctggtttcat acgccaaatc tgcattgttc ctgaagcact tccgttgtag taaatgtatt
     4201 ttccatcggg tgagtattca caaccgtcaa catgttctcc aggtttggtg gctgtaagtg
     4261 caacctcttc acctccttta acaggttttc tgtaaatgtt ataggtgc
//
LOCUS       DAOSNB010000221         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20620_length_4284_cov_4.386380, whole genome shotgun sequence.
ACCESSION   DAOSNB010000221 DAOSNB010000000
VERSION     DAOSNB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20620_length_4284_cov_4.386380"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLG42_08760"
     CDS             <1..781
                     /locus_tag="PLG42_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOU31337.1"
                     /translation="KREGVEVIIALDVSNSMLAEYIQPNRLERAKQALSRLIETLEND
                     RIGLIVFAGDAYIQLPITTDYVSAKMFLSTINPSLVPKQGTAIGAAIRLGMRSFSPGQ
                     DKSRAMIIITDGENHEDDPVEAAKEAAEAGIVIHTIGIGSPEGVPIMMNVGGRREYLK
                     DHEGNTVITKLNENILKEIAINANGKYVRATTTNLGIDQIFSEIRKMKKQELEGKIFT
                     EYNDQFQFFAGASLILLLFDFLIMERKNRKLSGLKLFKLKI"
     gene            789..1532
                     /locus_tag="PLG42_08765"
     CDS             789..1532
                     /locus_tag="PLG42_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOU31338.1"
                     /translation="MTEKNKYYLRVFLAALFLQSFVISSVSGQGEKRYLRQGNSKYFD
                     GNYSESEILYRKAIEENRSSAAGNFNLGDALYRQNKFDDASKQFILSADMSADRIKKA
                     ESFFNLGNSLLKSNKIEESIEAYKNSLRLNPTSLEAKYNLAYAQDLLKKMQQQQQQQQ
                     KEQNQNKEQDQKEGKSDQQQQQQQNQDEKKQNQQEQQQQQMQQQKQGISKEDAERILS
                     ALENDEKKVQEKVKQANALRERVRTLKNW"
     gene            1536..3350
                     /locus_tag="PLG42_08770"
     CDS             1536..3350
                     /locus_tag="PLG42_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOU31339.1"
                     /translation="MRRLIIKYLFILIVPSVAVAQEIVIKTEYPRVVSVGEQFSISWT
                     INAGGGEFSAPPFTGFYKLMGPQTSYSSSMQIINGRISQETSYTYTYYLQAIEEGKFV
                     IPPAIIRVKGKEYRSDSIYIEVVGTPGARQMQPSASRKQATEPAAVEPSGELFVRLIP
                     NRTELYVGEPLTVSLKLFSRVDLAGLEEVKYPDFSGFVKEELETPQLTSLQRENVNGV
                     IYGTGIIQQFLLFPQITGEITIDPVQVTALVQQRIGEPDPFFGDFFSRFANVPRVLAT
                     LPLKITVKPLPENKPADFSGIVGKINISASLNKDTVKVNDALNFKITVSGTGNLRLAA
                     KPVIKLSPDIEIYDPKITDNLRNNTSGITGQRTYEYVLIPRHHGDFVIPSVTYSYFNP
                     ATKQFETATTKEFRFHALKTDEQSGEVTVFGGVTREDVKYLGKDIRFIKSLPGIIIIP
                     ERLLLSNRMFTWYYVLALLIFATVLILRREHVRRNADIARVRNRRAGKVAKKRLSVAY
                     KCLKSGMKEKFYEELLKALWGYMSDKLGIPLSELTRTKAVEKLKERNVDDQIIEKISS
                     IIDRCEYARFAPVSAPSEAEELYEGAAQFIRYIENKLD"
     gene            3358..4122
                     /locus_tag="PLG42_08775"
     CDS             3358..4122
                     /locus_tag="PLG42_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOU31340.1"
                     /translation="MKKIILLIILALFNFNSEIGAAVFPEDDFRKGIELYTSGKYQEA
                     LDVWLPIYKSGFRTPNLCYNIGNAYFKINDVPHAILFYERAYLLKPADEDIRYNLQVA
                     RTYVVDRFTEIPELFFVTWYNFISLILSSDKWAYISIISFVMSLIFMLVYLLTSVYRY
                     KVAGFWLAIFMLIISLASLSFSIRNKNLVFNSDKAIIVSPQVNGKSAPDSSGTDLFLI
                     HEGTKVTVTDALGEWYEIKLPDGNKGWVPANSLEKI"
BASE COUNT         1457 a          747 c          917 g         1163 t
ORIGIN      
        1 aaaaagagag ggtgttgaag tgataatagc actcgatgtc agtaattcaa tgcttgctga
       61 atatatacaa cccaacaggc tggagagggc taagcaggct ctttcacggc ttattgaaac
      121 tcttgaaaat gacaggattg gccttattgt ttttgccggc gatgcctata tacaattacc
      181 tataacaacc gattatgtat cagcaaaaat gtttctttca actataaatc cttccctggt
      241 acccaaacag ggtacagcaa taggtgcagc aatacgtctt ggaatgaggt ccttttctcc
      301 cgggcaggat aaaagtcgtg cgatgattat aattaccgat ggcgaaaacc atgaggatga
      361 tccggttgaa gcagcaaaag aagcagccga agcaggaata gttatccata ccatcggaat
      421 aggttcacca gaaggagttc ctataatgat gaatgtaggt gggagaaggg agtatctgaa
      481 agaccatgaa ggcaacactg ttatcaccaa actcaatgaa aacattctta aagagatagc
      541 tattaatgca aacggtaaat atgtaagggc aacaacaact aatctgggta tcgaccagat
      601 attttccgag ataagaaaaa tgaaaaaaca ggaacttgaa ggcaaaatat ttacagagta
      661 caacgaccag ttccagtttt ttgctggagc ttcactgatt ctgcttttat ttgactttct
      721 aattatggaa aggaaaaaca ggaaactatc aggtcttaaa cttttcaaac tgaaaatatg
      781 aaaaggttat gactgaaaaa aataaatatt atttacgggt atttttagca gcgctatttc
      841 tgcagtcgtt tgtcatttct tcagtttcag ggcagggtga aaagaggtac ctgcgtcagg
      901 gaaattcgaa gtattttgat ggaaattatt ctgaatctga aatactttac aggaaggcga
      961 tagaagagaa tcgctcttct gctgcaggaa attttaatct tggtgacgca ctttaccggc
     1021 agaataaatt cgatgatgca tcgaaacagt tcatcttgtc agctgatatg agtgccgaca
     1081 ggattaagaa ggcagaaagc ttctttaatc tgggtaattc tttactgaaa agcaataaaa
     1141 tcgaagagag tattgaagca tataaaaatt cactgcgtct taatccaaca agtcttgaag
     1201 cgaaatataa tctggcatat gcccaggatt tgctgaaaaa aatgcagcaa caacagcagc
     1261 aacaacaaaa ggagcaaaat caaaataagg aacaggatca gaaagaaggt aagagcgatc
     1321 agcaacagca acaacagcaa aatcaggacg aaaaaaaaca gaatcagcag gagcagcaac
     1381 aacagcagat gcagcagcag aaacagggaa tatcaaagga agatgcggaa cgaattttga
     1441 gtgctcttga gaatgatgag aaaaaggtgc aggaaaaagt taaacaggcc aatgcattga
     1501 gggaaagagt gaggacattg aaaaactggt agtttatgag gcggttaata atcaaatatc
     1561 tgtttattct aatagtgcca tcagtagctg tggcacagga gatagttatc aaaaccgaat
     1621 acccgagagt ggttagtgtt ggtgaacaat tttcaattag ctggacaata aacgctggag
     1681 gtggtgagtt cagtgcacca ccatttacag gtttttacaa actgatgggg ccccagactt
     1741 catattcttc gagtatgcag ataataaacg gaagaatatc acaggagact tcatacacct
     1801 atacatatta tttacaggct attgaagaag gtaagttcgt tatacctcca gctattataa
     1861 gggttaaagg aaaagaatac aggtcggatt caatatatat tgaagtagta ggtacaccgg
     1921 gggcgcgtca gatgcaacct tctgcatccc gaaaacaagc aacagagccc gctgctgttg
     1981 aaccatcagg cgaattgttt gtaagattga tcccgaaccg caccgaatta tatgttggag
     2041 agcctttgac agtgtcgttg aaactttttt caagggtaga ccttgcaggt ctggaagaag
     2101 ttaaatatcc tgatttcagc gggttcgtga aagaagaact cgaaacgcca cagcttactt
     2161 cactgcagcg tgaaaacgtt aatggggtaa tatacggaac aggaattatt caacagtttc
     2221 ttttattccc ccagataaca ggagaaataa ctattgaccc ggttcaggta actgcactgg
     2281 ttcagcaaag aataggcgaa ccagacccgt tttttggaga cttcttttcc cggtttgcaa
     2341 atgtacccag agtgctggca acattaccat tgaaaataac tgtgaaaccc ctgcctgaaa
     2401 ataaacctgc cgatttttca ggaattgtcg gaaaaataaa catatcggca tcacttaata
     2461 aagatacagt caaggttaat gatgctctca attttaaaat aactgtatca ggaaccggta
     2521 atctaaggct tgctgcaaaa ccggttatta aactatctcc agatattgaa atttatgatc
     2581 ctaaaatcac cgataacctt aggaacaata cctccggaat tactggacag aggacttatg
     2641 aatacgttct catcccacgg caccatggtg actttgtaat accatccgtg acatactcat
     2701 atttcaaccc ggcaacaaaa caatttgaaa cggcaacaac aaaagaattc agatttcatg
     2761 ctttgaaaac cgatgaacag tccggagagg ttactgtttt cggaggagta acaagagaag
     2821 atgtaaaata tcttggaaaa gatattaggt ttataaaatc actgccgggc attattatta
     2881 ttccagaaag acttcttttg tcaaaccgca tgttcacgtg gtattatgtc cttgcacttt
     2941 tgatttttgc aactgtcctt atactcagac gtgagcatgt aaggcgtaat gctgatattg
     3001 caagagtaag aaacagaaga gccgggaaag ttgcaaagaa acgtttgtcg gtagcctata
     3061 aatgccttaa atcgggcatg aaggaaaagt tctatgagga acttctaaag gcattatggg
     3121 gctacatgag tgacaaactg ggaatacctc tttcagaact tacacgcaca aaagctgttg
     3181 agaaacttaa agaaagaaat gttgatgatc agataattga aaagatttct tcaattattg
     3241 acaggtgtga atatgcaagg tttgcaccgg tttctgctcc ttcagaagca gaagaactgt
     3301 atgagggagc tgcacagttt atcagatata ttgaaaataa acttgattaa tgtgaagatg
     3361 aaaaagatta ttctgctgat aattctggca ctatttaact tcaattctga aatcggagcg
     3421 gctgtttttc ctgaagatga cttcaggaag ggcatagaat tatatacttc cggcaaatac
     3481 caggaagctc tggatgtatg gctgccgata tataaatccg gattcagaac gcccaatctc
     3541 tgctataata tcggtaatgc atatttcaag ataaatgatg tgccacatgc aatactcttt
     3601 tatgaaaggg cataccttct taagccggcc gatgaagata taagatataa cttgcaggta
     3661 gccagaactt atgtagttga cagatttacc gagatacctg aattgttttt cgttacatgg
     3721 tataatttca tttcattgat actctcatct gataaatggg cttatattag catcatcagc
     3781 tttgttatga gtctgatttt tatgttggtc tatttgttaa cttcagtata caggtacaag
     3841 gttgcaggtt tctggctggc aatttttatg ttgataataa gccttgcatc gctcagcttt
     3901 tctataagaa acaagaacct tgtttttaat agtgacaaag caataattgt cagccctcag
     3961 gttaatggga aaagtgcccc tgatagcagt gggacagatc tttttcttat tcatgaaggg
     4021 acaaaagtta cagttactga tgctctcgga gaatggtatg aaattaaact acccgacgga
     4081 aataaaggat gggttcctgc aaatagtctg gagaaaattt aatgatacat gcaacttatt
     4141 ttgtaacttt catgtcaatt aattacaaaa tctgacagaa taatgaaaag gtatctcatt
     4201 tttgcagtga ttttactgct tgctttgagt ggttgcaaaa agctcgaaaa ggaaccctat
     4261 gggccgacag acataagaat aaga
//
LOCUS       DAOSNB010000222         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20774_length_4263_cov_3.786359, whole genome shotgun sequence.
ACCESSION   DAOSNB010000222 DAOSNB010000000
VERSION     DAOSNB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20774_length_4263_cov_3.786359"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="PLG42_08780"
     CDS             <1..1435
                     /locus_tag="PLG42_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOU31341.1"
                     /translation="TNRIGGRITGSDAYDNAVKWCAEQFKKWGMEVILDEVGTLPVGF
                     NRGPWFGRMLSEDGMILHFATPSYTAGTKGVQRGHVLMEPKTRAEFERMKGALKGAWV
                     LITGTNNGWPIDWSATGDSIRAKIIEENEEIERKNREIAAENRKKPESEQQPLIPLKD
                     EPALFYKEMREAGILGIIQSSAVPIRALYDRKNIEKMSFDNLPTVPDIKLDEHQYKII
                     EQMVKERRYFQLEFDIRNHFRPGPVKYYNVIGVIRGTKYPDEYVIMGGHLDSFDVATG
                     GVDDGSGVTPAMEAARLIMKAGGKPKRTILVTLWAAEEFGLWGSKSWVTRNKDKLGRI
                     SNYFNRDGGPTVANSVTVPEAMYEDFLKVCEPLKDINPKFPFTVNKRTTPPGPRPVTA
                     GGSDHAYFAMNGVPTIAFGTADPEGYNFNYGEIWHTERDTYDKSIAEYQNHTAIVTAV
                     VVYGLANLDHLLSREGLYSDEAEARKQ"
     gene            1538..2218
                     /locus_tag="PLG42_08785"
     CDS             1538..2218
                     /locus_tag="PLG42_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31342.1"
                     /translation="MRIIKLTFIKLQASFIAILFSFGLKAQQVDTLKDVPSLLLPRFT
                     TGIVKLKSGVVNKGIMNYDLVGQQMIFLQRKQTLVLREPQLVDTIFLANRIFVPFEKG
                     FYELLAQEGNTALFRQHRAHFEIPGAQIGYGLTSKTVAPQYYQQIYGPTGAIDLTVPE
                     GSKVVDDSQYWVRINGEMNNFRNRKQFLKIFPGKQKELEKFISQNKIDFAKIEDVKKL
                     FLYTLSLK"
     gene            2338..3363
                     /locus_tag="PLG42_08790"
     CDS             2338..3363
                     /locus_tag="PLG42_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HOU31343.1"
                     /translation="MKTEFTAVICLLLMTALSFSCKKDDKNSPGGATGPTATTVDATN
                     VGQGWATLNGTINPNNKITYVTFEYDTTTAYKLKAEADPDTISDNKVTNVKAHLTGLL
                     PGTKYYFRVVAVNEGGSSNGGEKTFTTTNPRPIVINFNQDITYGSVVDIEGNTYRTVV
                     IGTMEWMAENLRTTKLNDGTPISFVPDASGWNSSTFTYPGYCWYNNDSVSYGALYNWY
                     SVNTGKLCPAGWHVPSDDEWSATTDFLGGKDVAGGKLKEKGLSHWFSPNAGATNESGF
                     TALPGGYRYPIGSFNGIGRFGYWWTSTSFSEGEAYYRSMSNSYPNIDRSSSSKKSGMS
                     IRCVKDN"
     gene            complement(3370..>3549)
                     /locus_tag="PLG42_08795"
     CDS             complement(3370..>3549)
                     /locus_tag="PLG42_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31344.1"
                     /translation="VLTKYNVSYVLTWRHPYSRRFATSIKGPFPEDFLNFFKWQKSLF
                     LSDIKDENIYGKKKR"
     assembly_gap    3550..3559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3560..>4263)
                     /locus_tag="PLG42_08800"
     CDS             complement(<3560..>4263)
                     /locus_tag="PLG42_08800"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31345.1"
                     /translation="FELGHLELGDERSIDSVLFDHIAEQIKEHYRRGGVITISWHAKN
                     PLTIQDLASASRRQGGTAWDVSSKEVVSSILPGGRNHELFNTWLERLASFFMKLKDDD
                     GTPIPFIFRPYHEHSGSFFWWGTGLCSDEEYSALWRYTVSFLRDKKKIHNILYAYNTD
                     RVTSLEQYMKGYPGDDVIDMLSLDMYDRGPQFGPELDNALGFVTSEAARRKKLSALSE
                     TGPRRGMTDWWSSVLMP"
BASE COUNT         1311 a          821 c          987 g         1134 t
ORIGIN      
        1 tacaaacagg attggaggaa ggattaccgg ttcagatgcg tacgacaatg cagtgaagtg
       61 gtgtgctgag caatttaaaa aatggggcat ggaagtgata cttgacgagg ttggtacctt
      121 gcctgttggg ttcaaccgtg gaccctggtt tggaaggatg ctatctgaag atgggatgat
      181 acttcatttt gcaacaccct catatactgc aggtacaaaa ggtgtgcagc gagggcatgt
      241 gcttatggaa cctaaaacaa gggctgagtt tgaaaggatg aagggtgcac ttaaaggagc
      301 ctgggtgcta atcacaggaa ccaataacgg gtggcctatc gactggtcgg cgacaggcga
      361 ttcaataagg gcaaagatta ttgaggagaa tgaagagata gagaggaaga atcgtgagat
      421 tgcagccgaa aaccgcaaaa agcctgaaag tgagcagcaa cccctcatcc cgcttaagga
      481 tgagcctgca cttttttata aagagatgcg tgaagccgga attctaggaa taatacaatc
      541 gtcggctgta cctataagag cactgtacga caggaaaaac atcgaaaaaa tgtcatttga
      601 caatttgcca actgtgcccg atataaaact tgacgaacac cagtacaaga ttatcgagca
      661 gatggttaag gagagaaggt attttcagct cgaatttgat atacgcaacc atttcaggcc
      721 gggtccggta aagtattata atgtaatagg agttatccgg ggcacaaaat accctgatga
      781 atatgttatt atgggtgggc atctcgattc atttgatgtt gcgacaggag gggtggacga
      841 tggttcgggt gtaacacctg caatggaagc agcacgcctt ataatgaagg caggtggcaa
      901 accgaaaaga actattcttg ttactttgtg ggctgctgag gagttcggac tctggggctc
      961 aaagagctgg gtaacaagaa acaaggataa actgggcagg atatcaaatt attttaaccg
     1021 cgatggaggt ccaaccgttg ccaacagtgt aacggttccc gaagcaatgt atgaagactt
     1081 cttaaaggtg tgtgaacctt tgaaagatat taatccgaaa ttcccgttca ctgtgaataa
     1141 aagaactact cctccaggtc ccaggccggt tactgcagga ggctccgacc atgcatattt
     1201 cgcaatgaac ggagttccca caatagcctt tggcacagcc gaccccgagg gttataattt
     1261 taactacgga gaaatatggc acactgaaag agacacttac gataaaagta tagctgaata
     1321 tcaaaatcat acggcaattg tgacggcagt tgtagtttat ggccttgcaa atctcgacca
     1381 cctgctctca agagaaggtc tctacagcga tgaggccgaa gccagaaaac aataaatatg
     1441 aaaaaagcca taaatattat taaaaacaat gaatggcagg atttttgcaa taattaaacc
     1501 gccattctgt ttattcttag ataacccggt taagattatg aggataatta aactgacatt
     1561 tataaaacta caggcttctt ttattgccat attattcagc tttggactta aagcccagca
     1621 ggtggatact cttaaagatg ttccaagtct tcttctgccc cgttttacaa cgggaattgt
     1681 caagcttaag tcgggagtgg tcaataaagg cattatgaat tatgatcttg tgggacaaca
     1741 aatgattttt cttcagcgaa aacagactct ggttctgaga gagccccagc ttgtcgatac
     1801 aatatttctt gccaacagaa tttttgtgcc gtttgagaag ggtttttacg agttgcttgc
     1861 tcaggaaggg aatacggctc tttttcgtca gcacagggca cattttgaga tacctggtgc
     1921 acagataggt tatggtctga catcaaaaac agtagctccg cagtattatc agcagatata
     1981 cggtccaacc ggtgcaatag atctcaccgt gccggaagga tcaaaggtcg ttgacgatag
     2041 ccagtactgg gtcagaatta acggggagat gaataatttc aggaatagaa aacagtttct
     2101 taaaatcttc cctggaaaac aaaaggaact tgaaaaattt ataagccaga ataaaattga
     2161 ttttgcaaag attgaagatg taaaaaagtt gtttttgtac actttaagcc tgaagtgagc
     2221 tgagtataga tccatagata ctctcaggca ggcttataat tttgcagaaa attgaatttc
     2281 atgttgcttt aaatatatat atgatgaatt taaactgatt atgcgatggg ggaaaaaatg
     2341 aaaacagaat ttacagcagt tatttgtctg ttattgatga cagctctaag tttttcatgc
     2401 aagaaggatg ataaaaattc accgggtggt gcgacaggac ccactgcaac aacagtagat
     2461 gcaaccaatg tagggcaggg atgggctaca ctcaacggaa ccattaaccc taacaataag
     2521 attacgtatg ttacttttga atatgatacc acaacagctt ataagctgaa agctgaagct
     2581 gatccggata ccatatccga taataaagtt actaatgtaa aagcccacct gaccggactt
     2641 ttacccggga caaaatatta tttcagggtt gttgcagtga atgaaggggg ttcatcaaac
     2701 ggaggtgaaa agacatttac aactaccaat ccacgaccaa ttgtaataaa ctttaaccag
     2761 gatataacct atggttctgt agttgatatt gaaggtaata cctaccgcac agttgttatc
     2821 ggaacaatgg agtggatggc tgaaaacctt cgtacgacga aactgaatga tggaacacct
     2881 atatcatttg taccagatgc ttctggatgg aactcttcaa catttactta tccgggatat
     2941 tgctggtaca ataatgattc tgtctcatat ggagcccttt acaactggta ttcagtaaat
     3001 acaggcaaat tgtgtcctgc cggatggcat gtgccttcgg atgacgaatg gagtgccacg
     3061 acagattttc tgggagggaa agatgttgca ggcgggaaac tcaaggaaaa gggtttgagt
     3121 cactggtttt cccccaatgc aggtgctacg aatgaatcgg gcttcacagc cctgcccggg
     3181 gggtatcgtt atcccatcgg ttctttcaac ggtataggcc ggtttggtta ctggtggact
     3241 tcaacttcat tttccgaagg tgaagcatat tacagaagta tgtcaaacag ttatccgaat
     3301 atcgatagaa gtagcagcag taaaaagagt ggaatgtcaa ttcgttgcgt aaaagacaat
     3361 taataggaat tatcttttct ttttcccgta aatattttca tcttttatat cgctcagaaa
     3421 gagcgatttt tgccatttga agaaattaag aaaatcttcg gggaagggtc ctttaattga
     3481 tgttgcaaag cgccggctgt agggatggcg ccatgtgagc acataactta cattatattt
     3541 ggtgagaacn nnnnnnnnng gcatcagaac cgacgaccac cagtcggtca tccctcgccg
     3601 gggacctgtt tcactcaggg ctgaaagctt tttccttctt gcagcttcgg atgtaacaaa
     3661 tccgagtgca ttatcaagtt ctggtccgaa ttgaggacca cgatcataca tgtcgagcga
     3721 cagcatatcg ataacatcat ctccagggta acctttcata tattgttcaa ggcttgtaac
     3781 cctgtcggtg ttgtatgcat acagaatatt gtggattttc tttttatcgc gcagaaaact
     3841 tacagtatat ctccacaggg cagagtattc ctcatcggag cacaaaccgg tgccccacca
     3901 gaaaaatgaa ccggaatgtt catgatatgg cctgaaaata aaaggtatcg gagtgccatc
     3961 gtcatcttta agtttcatga aaaatgaagc aagtcgctca agccaggtat taaaaagttc
     4021 gtgatttctg ccacctggca gtattgatga aaccacttct tttgagctca cgtcccacgc
     4081 tgttccgccc tgacgccttg aggcagaggc cagatcctga attgtaagtg ggttttttgc
     4141 atgccagctg atagtaataa cgccacccct cctgtaatgc tcttttatct gttctgcaat
     4201 atgatcgaag agaaccgaat caatacttct ttcatcaccc agttctaaat gtccaagctc
     4261 aaa
//
LOCUS       DAOSNB010000223         4261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20788_length_4261_cov_4.635521, whole genome shotgun sequence.
ACCESSION   DAOSNB010000223 DAOSNB010000000
VERSION     DAOSNB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20788_length_4261_cov_4.635521"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLG42_08805"
     CDS             complement(<1..227)
                     /locus_tag="PLG42_08805"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOU31346.1"
                     /translation="MNTFGHIFRVSLSGESHGKAIGVTIDGCPPGIPVQPDDFLPDLS
                     RRRSGKKGTTPRREEDLPDILSGIYNGHTTGS"
     gene            complement(234..1493)
                     /gene="aroA"
                     /locus_tag="PLG42_08810"
     CDS             complement(234..1493)
                     /gene="aroA"
                     /locus_tag="PLG42_08810"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOU31347.1"
                     /translation="MKVEASVKEVRGRLRAPSSKSAVQRYIAGALLAEGLSTISVNSM
                     CDDSLAALSVATELGAEATTCGDSIKIKGGFNPKTNKINCGESGLATRMFIPIAAIHN
                     REITVTGRGSLMKRPVKMVEEPLRQLGVQISSKDGYLPVKIKGPLTGGEVHADGSISS
                     QFITGLLMALPVAQKNSCVIVENLVSRPYIDLTIKILQEFDIEIVNDSYRVFSIKGGQ
                     KYTPGTFTAEGDWSGAAFLLVMGAIGGKVEVTGLRTDSVQADKAILEPLMMAGASVSV
                     EAGRIKVEKKELNSFEFDISDCPDLAPPLAVLAAACNGKSVLRGAHRLVAKESNRAET
                     ITAALNAIGANIKTSGDIIEIYGNKTLSGGESSAYNDHRIAMALATASAISTSPVRIE
                     GMEAINKSYPGFIDDFRKLGGKIKIVK"
     gene            complement(1493..2530)
                     /gene="aroB"
                     /locus_tag="PLG42_08815"
     CDS             complement(1493..2530)
                     /gene="aroB"
                     /locus_tag="PLG42_08815"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOU31348.1"
                     /translation="MNRITINLNNSKSEIYIGLNWNEFSRFIPVQNTVIITDDNIKNI
                     YGSHFPQLPVFSVKPGEESKTLQVVTSLTERLLECGIDRSGFLVGIGGGVVCDLTGFL
                     ASIYMRGIRFGFVSTSLLSQVDASVGGKNAVNLGKVKNVLGCFRQPEFVICDPQMLNT
                     LPEEEYLSGLAELIKMGLIMDYNLFSLAEQNRGRLLNRDIELLEYMISVSVELKASVV
                     REDEKEVSGKRMILNFGHTFGHVIETMAEMKHGFAVAAGMVIASDISVQAGLLEPAAR
                     DRIVNLLKELKLIKEYNVPDAVFEEMLLKDKKKQGDNISFVLLESPGKAVISKINTRE
                     LMEFYKVLKRS"
     gene            2651..3301
                     /gene="nth"
                     /locus_tag="PLG42_08820"
     CDS             2651..3301
                     /gene="nth"
                     /locus_tag="PLG42_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOU31349.1"
                     /translation="MTTAERFRKVIEYFSRSNPVPETELRYRDPFGLLVAVMLSAQCT
                     DRRVNMITPVLLKAFPDPASLAAATQEQVVSLIKSCSYPNSKAKNLIKMANVLIEKFG
                     GKVPEEPELLQQLPGVGRKTANVVASVLFKKPVIAVDTHVFRVSVRLGLAVNAKTPLD
                     VEHQLEEHIPPEMRHKAHHWLILHGRYICRARKPLCEKCGLTSICKYYKDKNKASR"
     gene            3366..>4261
                     /locus_tag="PLG42_08825"
     CDS             3366..>4261
                     /locus_tag="PLG42_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOU31350.1"
                     /translation="MKRKFSEIGNIIAFNLRKFLPFRKNYQGEIALRVFHAAPQIMII
                     SNLKTGEIIDVNEAFVKNLDYKKNEVIGKNFDDLKLFSEMEESNKYIKLLPRFRKITD
                     YPVVLNTKKGEKKHYLFSAETIKLGNDYFLLTTYNPDPYSGKTSLQERADEILDDIFD
                     TVSSYLLRISILKDDRFCIKDINRKAEDVEGIVRSEVLGKPVEETALVKRPKLLELLH
                     HIRITGEPLKLAVSPAGDRSEGFYIGFLLSTGDIIVTWEPPENMQKLNERSIAEPLPG
                     MSKVMSFEIDTQGKITFASENSL"
BASE COUNT         1218 a          896 c          892 g         1255 t
ORIGIN      
        1 gagccagttg tgtgtccgtt atatattcca ctcagtatat cgggcaaatc ttcctcacgc
       61 cggggggtag ttcccttctt cccgctcctt cttctgctca ggtcgggcag gaaatcgtca
      121 ggttgaacgg gtattccggg cgggcatccg tcaatagtga cgccaatagc ttttccatgt
      181 gattcgccag agagggaaac cctgaaaata tgtccgaatg tattcataat ctgttatttg
      241 acaattttaa ttttgccgcc cagttttctg aagtcatcaa taaatccggg gtatgattta
      301 tttatagctt ccattccttc aattcgaaca ggtgatgttg aaatggctga agcggttgca
      361 agtgccatgg ctatgcgatg gtcgttgtaa gctgaggatt ctcctcccga tagagtttta
      421 tttccatata tttcgatgat gtcgccactg gttttgatat ttgcgcctat tgcgttgagt
      481 gcagccgtta ttgtttctgc tctgttactc tcttttgcaa caagtcggtg ggcgccacgc
      541 agaacacttt taccgttaca tgcagcagcc agtacagcca gcggcggtgc aagatcgggg
      601 caatcactga tatcgaattc aaaagaattt aactctttct tttctacttt tatcctgccg
      661 gcttcaacag aaacagatgc gcctgccatc atcaaaggct caaggattgc tttatcagcc
      721 tgaacggaat cagttctgag acctgtcact tccactttcc ctcctattgc tcccatcaca
      781 agcaggaagg ctgcaccgct ccagtcgccc tcagctgtaa acgtaccagg agtatatttc
      841 tgtccacctt taatgctgaa gactctgtaa ctgtcgttaa ctatctctat atcaaattct
      901 tgtaatattt tgatagtcaa atcaatatat ggcctgctaa caaggttttc aactataacg
      961 cagctatttt tctgagcgac cggcagagcc ataagcaaac ctgtgatgaa ttgtgagctt
     1021 atcgagccat cggcatgcac ttctccgcct gtaagaggtc ctttaatttt aactggaagg
     1081 tatccgtcct ttgatgagat ctggactccc agttgtcgaa ggggttcttc gaccattttt
     1141 acgggtcgtt tcataagtga gccgcgtcct gtgacggtaa tttctctatt gtgtattgca
     1201 gctatgggga taaacatccg ggtggcgagg cctgattcac cgcagttgat tttgttggtc
     1261 ttagggttga agcctccttt tattttgata gaatctccgc aggttgtggc ttcagcgcca
     1321 agttctgttg caacagagag tgctgcaagt gaatcgtcgc acatggaatt gactgatata
     1381 gtcgataaac cctctgcgag cagagcaccg gctatatatc tctgtaccgc actcttcgac
     1441 gatggagctc tcaaacgacc cctgacctcc ttaacagatg cttctacttt cattaacttc
     1501 tcttcagcac tttgtaaaat tccattaatt ctctggtatt gattttactt attacagctt
     1561 tccccggcga ttcaagcaga acgaaactaa tattatcgcc ctgtttcttt ttgtctttaa
     1621 gaagcatttc ttcaaagact gcatccggga cattgtattc ttttataagc ttcagttctt
     1681 tcagcagatt tacaattcta tcgcgtgcgg caggttcaag taagcctgcc tgaaccgaaa
     1741 tatctgatgc tatgaccatc cctgctgcaa cggcaaagcc gtgtttcatt tccgccatag
     1801 tctcaattac gtgtccgaat gtatgaccaa agttaaggat cattcttttg ccggaaactt
     1861 ctttttcgtc ttcccttaca acagaggctt tcagttcaac cgaaacagat atcatgtact
     1921 cgagcaattc aatgtctcta tttagaagtc gccctctatt ctgttctgca agagaaaaaa
     1981 ggttgtaatc cattatcagt cccattttta taagttctgc cagacccgag aggtattctt
     2041 cttcaggcaa cgtattaagc atttgcgggt cgcagataac aaattcgggc tgacggaaac
     2101 aacccagcac atttttgacc tttccaaggt ttacagcgtt ttttccacca acacttgcat
     2161 caacctgcga caaaagtgat gttgacacaa atccaaacct gatacctctc atataaatcg
     2221 aagcaaggaa tccggttaaa tcgcaaacaa cgcctccccc tattcccaca agaaaccccg
     2281 atctatcgat accacattca agaagtcttt cggtaaggct ggtaacaacc tgaagagttt
     2341 tgctctcttc tccgggtttt acagaaaaga caggaagctg gggaaaatga cttccgtaaa
     2401 tatttttgat attatcgtcg gtaatgataa cagtattttg caccgggatg aaacgggaaa
     2461 actcattcca gttcagtccg atatatattt ctgatttaga attattcaga tttatcgtta
     2521 tcctgttcaa agtctttaaa attatttttt aaatataaga aatccttaat ctttttcaga
     2581 tgcagaaaaa agaatattca aaaataaaaa gtaaaatatt actttaatac gatttttcat
     2641 gcatgatatt atgacgacag ctgaaaggtt cagaaaggtt attgagtatt tcagcaggag
     2701 taatcctgtg cccgagaccg aactcaggta cagggatccg ttcgggttgc ttgtggcggt
     2761 gatgctttcg gctcaatgta ccgacaggag ggtaaacatg attacgccgg tattgcttaa
     2821 agcttttcct gatccagcat ctctggcagc agctacacag gaacaggtcg tcagtctgat
     2881 aaaatcatgt tcttatccca acagcaaggc aaagaacctg ataaaaatgg ccaatgtgct
     2941 aattgaaaaa ttcggtggga aggttcctga agaacctgag ttgctgcaac aattaccggg
     3001 tgtgggaagg aaaactgcaa atgtggtggc gtctgtactc ttcaaaaagc ctgttattgc
     3061 tgttgacaca catgtattca gagtgtcggt tcgtctggga cttgctgtta atgcaaagac
     3121 accgctcgat gtggaacatc agctcgaaga acatatccct cctgaaatga ggcacaaagc
     3181 ccaccactgg ctgatattgc acggaagata tatctgcagg gcgcgaaagc cattatgtga
     3241 gaaatgcggg ttaacctcaa tatgcaaata ttataaagac aaaaataagg catcgcgata
     3301 gccatattaa aacatattga ttatttttga ttatcatttc taaagtggga gttgggaatt
     3361 ttaagatgaa aaggaagttc tcggaaatcg gaaatataat tgctttcaat cttcggaagt
     3421 tcttaccatt caggaagaat tatcagggtg aaattgcttt aagggttttt catgcagccc
     3481 ctcagataat gattatcagt aacctgaaga ccggagaaat aattgacgta aacgaagctt
     3541 ttgtaaagaa cctggattac aaaaagaatg aagtaatcgg aaagaatttc gatgacctga
     3601 agctcttttc agaaatggaa gagagcaata agtacataaa gcttcttccc cggtttcgca
     3661 aaataacaga ttatcctgtt gtactgaata caaagaaagg tgaaaagaaa cattatctgt
     3721 tctctgctga aacaattaaa cttggtaacg attattttct gcttaccaca tacaatccgg
     3781 atccatattc aggaaaaaca tctttacagg aaagagctga tgagatcctc gatgatattt
     3841 tcgatacagt ttcgagttat cttctgcgta taagtatttt gaaggatgat agattctgca
     3901 taaaggatat aaacagaaaa gcggaagatg ttgaaggtat tgtaaggtct gaagttctgg
     3961 gtaaacctgt agaggagaca gcgctggtga aaaggccgaa gcttcttgaa ttgcttcatc
     4021 atataagaat aaccggtgaa ccactcaaac ttgctgtttc gcctgcagga gacagatctg
     4081 aaggctttta cataggtttc ctgctttcaa caggagacat cattgtaaca tgggaaccac
     4141 ctgaaaacat gcaaaaactg aatgagaggt ccattgcaga acctttaccg ggcatgtcga
     4201 aagtgatgag ttttgaaatt gatactcagg gaaagataac tttcgccagt gaaaatagtc
     4261 t
//
LOCUS       DAOSNB010000224         4243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20907_length_4243_cov_4.831901, whole genome shotgun sequence.
ACCESSION   DAOSNB010000224 DAOSNB010000000
VERSION     DAOSNB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20907_length_4243_cov_4.831901"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2301
                     /locus_tag="PLG42_08830"
     CDS             <1..2301
                     /locus_tag="PLG42_08830"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31351.1"
                     /translation="QVKPPLTSGNEKVQLFEKHKMMQQNSPYKDLVWQYLGPTNISGR
                     CTDVEAISPRGKNYTIWIASASGGVWKSINEGTTFIPVFDAMPTASIGDIAIDPNDPN
                     VVWVGTGEANIFRSSNAGCGVFKTTDGGKTWSNMGLENTFTIGRIRINPKNSNIVYVA
                     ATGHEWTPNPERGLFKTTDGGKTWDKILYIDQNTGVFDLLLDPRDPDIIYCTTWERIR
                     LKWNDPRTYETTKNNGIWKSTDGGRNWKKINEGLPAPNKMGRIGIDLCLTKPDVLYAL
                     VDNYEVAYKAQPGELDSYGRPRADVIKGATVYRSDNAGGSWKQVSGLTPDQKTYMERH
                     SGTYGWVFGQIRVDPLDENTVYTLGINLNQSTDGGKTFRPLRGPHVDHHGLWIDPENP
                     NYLLDVQDGGLTISYDKGATWKYPIEPLPLAQFYNVAYDMSTPFRVFGSIQDHHSFYA
                     PVDLSRGRDRVPAQEFKNTLGAEGSMHAINPVDNNTIYASSFYGRLQRGKIDTYPRET
                     KSLLPNTYPDETPLRGEWNAPTLISPHNPNIIYHGMQYVLMSKDGGDTWEIISPDLSY
                     NDPKKRGDINYQTISVIDESPLRFGLIYAGTDDGRIWRTKDEGKTWTEIRNGAVPQKF
                     VSRIVASKFNIGTVYMTQTGRRDDDFQVYIWKSTDFGTTWKDISGNIPLGPVNVIRED
                     PVNPDILYVGTDCAVYVTRDGGNRWDVLGTLPYVYVHDLIIHPRDNMIVIATHGRGMW
                     VMDANPVNEKDKRARQREATEPAEDM"
     gene            complement(2307..3758)
                     /locus_tag="PLG42_08835"
     CDS             complement(2307..3758)
                     /locus_tag="PLG42_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOU31352.1"
                     /translation="MKKVLRLFLWLVAVLLIILIVKALFFKSLQANFGEVTTVGFGTE
                     SLLNLSEAVKYPTVSYAPGLPVDTSAFQGYIGFIRRKYPLVDSLLHLEVFNKFSLLYR
                     WDGRNASLLPVILMAHYDVVPPGDTAGWEKGPFSGLNDSTFIWGRGTLDDKAAMISIL
                     EAVERLLSEGFVPERTIYLSFGHDEEISGEKGARAIAAYLEEKGVKAEYVLDEGMAVT
                     VGMVPMIKEPVALVGTSEKGYLSVRLTAEMSGGHSSTPEKESALTVIAKAVGALAAKQ
                     MKPRLAGPVNDFIRYIGPEMPVFARVIFANKWLFKPLLLSIYSGSASGNALVRTTTAP
                     TIIQAGIKDNVIPTRAEAVVNFRILPGESSADVLEHIRKVIDDSRVIIEPLKDFINEP
                     APFSPTDAAGFQVIFKTLRQVYPEAYVAPTLTLSATDSRHFSVITDNIYRFAPIRVTA
                     EDMARVHGLNERTKIEDYLRGISFYYMLIKNSQ"
BASE COUNT         1312 a         1060 c          885 g          986 t
ORIGIN      
        1 caggtaaaac cacccctgac cagcggaaac gaaaaagtac agctgtttga aaaacacaaa
       61 atgatgcagc aaaactcacc atacaaagac cttgtgtggc aatacctcgg accaacaaac
      121 atcagcggac gctgcaccga cgtcgaagca atatccccac gaggaaaaaa ctatacaatc
      181 tggatagcct ccgcttcagg cggagtatgg aaatccataa atgagggtac aacctttata
      241 cccgtcttcg acgccatgcc aacagcctca ataggtgata tcgccatcga tccaaacgac
      301 cccaacgttg tatgggtcgg caccggcgag gcaaacatct tcagaagctc aaacgccgga
      361 tgcggtgtat ttaaaactac cgacggcggt aaaacatggt caaatatggg actagaaaac
      421 accttcacca taggcagaat acgtatcaac cccaaaaact caaatattgt ttacgtagca
      481 gctaccggcc acgaatggac tcctaaccct gaaagaggcc tcttcaaaac taccgacggc
      541 ggtaaaacat gggataaaat cctgtatatc gaccagaata ccggagtatt cgacctcctt
      601 ctcgacccaa gagaccccga cataatatat tgtaccacat gggaacgtat ccgccttaaa
      661 tggaacgacc caagaacata cgaaacgaca aaaaacaacg gcatctggaa atctacagac
      721 ggcggcagaa actggaaaaa aataaatgaa ggcctgccag cacccaacaa aatgggaaga
      781 ataggcatcg acctatgtct tacaaaaccc gatgtacttt acgcccttgt cgacaattac
      841 gaagtagcat acaaagccca gcccggcgaa ctcgattcat acggcagacc aagagccgac
      901 gtaatcaaag gcgcaaccgt ttaccggagc gacaatgccg gagggtcgtg gaaacaggtg
      961 agcggcctta cacccgacca gaaaacatat atggaacgcc attcaggcac atacggctgg
     1021 gtattcggac aaattcgtgt tgaccctctt gacgaaaaca cagtctatac cctcggaata
     1081 aatcttaatc aatcgaccga cggaggtaaa actttccggc cattgcgtgg acctcacgtc
     1141 gaccaccatg gactatggat agaccctgaa aacccaaact acctgcttga cgttcaggat
     1201 ggtggactga ctatttcgta cgataaagga gctacatgga aataccctat cgaacccctc
     1261 ccgcttgctc aattctataa cgtagcatac gacatgagca caccattcag agtattcggc
     1321 tcaatacagg accaccacag cttctatgca cctgtcgacc tttcaagagg aagagacaga
     1381 gtgcctgcac aggaattcaa aaacaccctt ggagccgaag gaagtatgca cgcaataaac
     1441 cccgtagata acaataccat ttatgcaagt tccttctacg gcagactgca acgggggaaa
     1501 atagatactt acccacgcga aacaaaaagc cttcttccaa atacttatcc cgatgaaaca
     1561 cctttaagag gggaatggaa cgctccaact ttgatctctc ctcataaccc aaatatcatt
     1621 tatcacggaa tgcagtacgt tttgatgtct aaagacggcg gtgatacctg ggagataatc
     1681 agccccgacc tgtcgtacaa cgaccccaaa aaacggggtg acataaatta ccagacaatc
     1741 tcagtaatcg atgaatcacc tctcagattc ggacttatct atgcaggtac cgacgacggc
     1801 agaatatgga gaacaaagga cgaaggcaaa acctggacag aaatacgcaa cggagcagta
     1861 cctcagaaat ttgtttcccg tattgttgct tcaaaattta acatcggaac tgtttatatg
     1921 acacaaacag gcagaaggga tgatgatttt caggtctata tctggaaatc aaccgatttt
     1981 ggaactacat ggaaagatat atccggcaat attcctctcg ggcctgtaaa cgtcataagg
     2041 gaagaccccg taaaccctga cattctttat gttggaaccg actgtgccgt ttacgtcacc
     2101 agggatggcg gtaacaggtg ggatgtactg ggaacactac catatgttta tgttcacgac
     2161 ctgataatcc atccccgcga taacatgatt gtcattgcta cacatggccg cgggatgtgg
     2221 gtaatggatg ccaaccctgt caacgaaaaa gataaaaggg ctcgacagcg ggaggcaact
     2281 gagcctgcag aagacatgta ataaaactat tgtgagtttt tgatcagcat gtaatagaaa
     2341 ctgatcccgc gaaggtaatc ttcaattttt gtccgctcat tcaacccgtg tacccgggcc
     2401 atgtcttcag cggtaactct gatgggtgca aaacggtaaa tgttatctgt gataaccgaa
     2461 aagtggcggc tatcggtggc actaagtgta agggttggtg caacataagc ctcaggataa
     2521 acctgcctga gggttttgaa aatcacctgg aaacccgctg catctgtcgg tgaaaaaggc
     2581 gcaggctcgt tgataaaatc tttcagcggc tcaataatta cacgtgagtc atcaataact
     2641 tttcttatat gctcaagaac atctgctgaa ctctcacctg gcaaaatcct gaaattcaca
     2701 actgcttctg cccttgttgg aataacattg tcttttattc cggcctgaat aatagttgga
     2761 gctgttgtgg ttcttacaag tgcatttcct gaagcactcc cggaatatat actcagaagc
     2821 agaggtttga aaagccattt gtttgcaaaa atcactcttg caaaaaccgg catctcaggg
     2881 ccaatatacc ggatgaaatc atttaccggt ccggcaagac ggggtttcat ctgttttgca
     2941 gccagagcac caacagcctt tgcaataaca gtaagggctg attctttctc gggtgttgag
     3001 gagtggccgc cagacatctc tgcagtcagt cttacagata gatagccttt ttccgaagtg
     3061 cccaccagag ccacaggctc cttaatcatc ggcaccatcc cgacagtcac cgccataccc
     3121 tcatcaagca catattcagc cttaacgcct ttctcctcca gatatgctgc aatagccctg
     3181 gctcctttct caccgctgat ctcttcatcg tgaccaaaag aaagatatat agtcctctca
     3241 ggaacaaatc cctctgagag aagtctttcc acagcttcaa ggattgatat cattgcagcc
     3301 ttatcatcga gtgtgccacg tccccagatg aaagtgctat cgttcagccc cgaaaaagga
     3361 cctttctccc accctgcagt atctcctggt ggaacaacat cataatgagc cataagaata
     3421 accggcaaaa gtgaagcatt cctgccgtcc cagcggtaaa gaagactgaa tttattgaag
     3481 acctccaggt gaagaagtga atcaaccagt ggatattttc ttctgataaa tccaatgtaa
     3541 ccctgaaaag ccgatgtatc taccggtaaa cccggcgcat aggaaacagt aggatattta
     3601 accgcttccg aaaggtttaa taaactttct gtaccaaacc caacagttgt aacctcaccg
     3661 aagtttgcct gaagtgattt gaaaaacaga gctttaacta ttaaaatgat gagcagcacc
     3721 gctactaacc agagaaaaag acgcaggact tttttcatat aagattattt aaaaacaata
     3781 caaatatcaa tataattttt ggggatttga attcgataat aattcgatag caatttaatg
     3841 gtaattcgat agtaattcaa tcgttccaga tacgcttcgc tgtcgggatc tccgtggaat
     3901 ttatcccgca aagcgtgact tcgaaactcg attgttcccg atacgtttgg ctatcaaaat
     3961 tttagcggag tttattcccc aaagaattat ctttattaat actcatccct cattcctcat
     4021 tcctcatccc tcattcctca tccctcaact atagccattc cgctttgtag gatttagttt
     4081 cacattttgt gattcacccc ccggcaccat gagagggtgc gaccccccgg caccatgaga
     4141 gggggcgacc ccgatcttct gtgagggagc gacccccaga ccccttaaga gggggctaga
     4201 ttaccactga tccccaaaat atcaggggct gtctaaaaag atg
//
LOCUS       DAOSNB010000225         4242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20918_length_4242_cov_3.938858, whole genome shotgun sequence.
ACCESSION   DAOSNB010000225 DAOSNB010000000
VERSION     DAOSNB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20918_length_4242_cov_3.938858"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLG42_08840"
     CDS             <1..449
                     /locus_tag="PLG42_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOU31353.1"
                     /translation="DYLFATSQLSQTTLRSILGQSELDELLSHREKINRELASIIDTQ
                     TEPWGIKVSNVEVKHIDLPQEMQRAMAKQAEAERERRAKVIHAEGEFQASQKLSEAAA
                     IISQNPVAVQLRYLQTVTEVAVDKNSTIIFPIPIELFKNFMADKGK"
     gene            461..3217
                     /locus_tag="PLG42_08845"
     CDS             461..3217
                     /locus_tag="PLG42_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU31354.1"
                     /translation="MRKISVCCFLLISFLFSRAQQTEIIYLSGIDKDITLQWDFFCTA
                     GRNSGYWTKIPVPSCWELQGFGTYNYGRDKQFADEKGLYRHKFTLPASVRGKRVFIVF
                     EGSMTDTEVKINGKPAGPVHQGAFYRFKYDITALVKSDSENLLETTVSKMSANKSVNQ
                     AEREADYWIFGGIFRPVYLEIVPDEFIEHVAIDARADGNFSASVRLNKNSAPKSIYAK
                     IETLDGNPVTELQEVNAPKGVTEVVIKGRVDNPGLWSPEFPNLYRVIISLKEGDKIRH
                     QVIQRFGFRTVEVRQNDGIYVNGQKIKFRGVCRHSSWPSSGKTTSKEISLLDVNLMKD
                     MNINAVRMSHYPPDQHFLDVCDSLGLFVIDELAGWQAFYDTEVGKKLVKEMVERDVNH
                     PCIILWSNGNEGGFNPELREEYAKYDPQKRLVIEPWHTLNGMNTKHYIPYNYGTATFF
                     NGRDIFFPTEFLHGLFDGGLGAGLEDYWNLMYSNPLSAGGFLWVFADEGVVRTDLNDS
                     IDTKGNLAPDGIVGPYREKEGSYYAIRDIWSPVWIEKKIITPQFDGRLKIENRFHYTS
                     LSQCSFSYYLVKLSDSFYPSEAYRISRTASPPSLLPGETGWLNLNLPDNWRDFDVIYL
                     NATDPHGRLINTWSWNISSPAEIASRIVKPEGAKAEGKDEGERVIIASGDVRVSINKS
                     TGLIEEVRKDQEIIPFTNGPRFTGLNPSFRELKHYSSGDNYVVELIYSNRPECHVKYT
                     MMPGGWLKLEWDYKPTGTADLFGITFSFPEEQVTGAILEANGPYHVWKNRLRGTLFGI
                     YDKKYNNTVTGESWDYPEFKGYYSNFYQVKVLTKNLPFTVVSATEDLYLHLFTPQKPR
                     HAAGYVTPPFPEGDISFLNGISAIGTKFLSPEQLGPQSQKNIYLLNRNTEPLAGCVYI
                     KFGE"
     gene            3481..>4242
                     /locus_tag="PLG42_08850"
     CDS             3481..>4242
                     /locus_tag="PLG42_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU31355.1"
                     /translation="MKTSVFFVSLALMLSVISCNTSDKKAGTAARVDVSEFLGQWTLD
                     IEGGTVGWLEVRQEEGYLDADLLWIAGSVTPVANVFLAANQYLVVTRTNNLIRKRDEA
                     GQPLRTHVVTNWLEVRKDGAEKLTGYLLTPRRNGLGVDSTSFTGKKLPPVPPAPDLSK
                     VKYGEPIVLFNGKDLTGWRLIEENTTNGFKVVDGVLVNDPVQPEGQPRIRYGNIRTEQ
                     EFEDFNLKLEVNVPEGSNSGIYLRGMYEVQVMDSYK"
BASE COUNT         1237 a          923 c         1041 g         1041 t
ORIGIN      
        1 aggattacct ttttgcaaca tcacagctat cacaaacaac tttgcgaagc atcctcggtc
       61 agtcggagct tgatgagtta ctttcgcacc gcgagaagat aaaccgggag ctggcgtcaa
      121 tcattgatac ccagactgaa ccatggggga taaaagtatc taatgtggag gtaaagcata
      181 ttgacctgcc gcaggagatg cagcgggcga tggcaaagca ggcagaagct gagcgagaac
      241 gccgcgcaaa ggtcattcat gccgaagggg aatttcaggc aagccagaaa ctgtcggagg
      301 ctgctgcaat aataagccag aatccggttg cagtgcagct tcgatacctg caaaccgtaa
      361 cggaagtggc agtcgataag aactcaacca tcatctttcc tatcccgata gaacttttta
      421 agaattttat ggcagataag gggaaataac ctgaatcgga atgagaaaaa tatcagtttg
      481 ctgttttctg ctgatctcat ttcttttttc ccgcgcacag cagacagaaa ttatctacct
      541 ctcaggcatt gacaaggaca tcaccttgca gtgggatttt ttctgcaccg ccggccgtaa
      601 cagcggttac tggactaaaa tccctgttcc atcgtgctgg gaactccagg ggttcggtac
      661 ctacaactat ggacgcgaca aacaatttgc cgatgaaaaa ggactgtacc gacataagtt
      721 caccctgccg gcgtcagtgc gtggaaaacg tgttttcatc gtcttcgaag gctccatgac
      781 cgacactgag gttaagatta acggcaaacc tgccgggccg gttcaccagg gggccttcta
      841 caggttcaaa tacgatataa cggctctggt caaatcagac tccgaaaacc tcctcgagac
      901 aacagtaagc aaaatgtcgg caaacaaatc ggttaaccag gccgaacgcg aagccgatta
      961 ctggatcttt ggcggaatat tccggcctgt ttacctggaa atagtacccg atgaattcat
     1021 tgaacatgtt gccattgacg cccgtgccga cggcaacttc tctgcatcag taagactgaa
     1081 taaaaactct gctcccaaaa gtatttatgc aaagattgaa acccttgatg gcaatcctgt
     1141 tactgaatta caggaagtca atgctccgaa aggcgtaact gaagttgtaa taaaaggcag
     1201 ggttgacaat cccgggctgt ggagtccaga attcccaaat ctttacaggg tgattataag
     1261 cctgaaagaa ggtgataaaa tcagacatca ggtaatacag agattcggat tcagaacggt
     1321 ggaagtcagg cagaacgatg gcatttatgt aaatggacag aagataaagt tcaggggtgt
     1381 gtgccgtcac agttcgtggc cttcatcggg aaagaccaca agcaaggaga tcagtcttct
     1441 cgatgttaat ctgatgaagg acatgaacat caatgccgtg cgcatgagcc attacccacc
     1501 cgaccagcac ttccttgatg tgtgcgattc gctgggtctg tttgtgattg acgaacttgc
     1561 gggatggcag gctttctatg atacagaggt gggcaaaaaa ctggtgaagg agatggttga
     1621 aagggatgta aaccaccctt gtataatttt atggagtaac ggaaatgaag gaggctttaa
     1681 tcctgagcta agggaggaat atgcgaagta tgatccgcag aagcggctgg tgattgagcc
     1741 gtggcacacg ctcaacggga tgaacacaaa gcattacata ccatacaatt atggcactgc
     1801 aacatttttc aacggcaggg acattttctt ccccaccgag tttctgcacg ggctgtttga
     1861 tggtggactg ggagcaggtc tggaagacta ctggaacctg atgtattcaa acccgctatc
     1921 agccggaggc tttctgtggg tctttgccga tgagggtgtc gtccgcacgg atttgaacga
     1981 ctcaatcgac accaaaggta acctggcacc cgatggaatt gttggcccat accgggaaaa
     2041 ggaaggcagc tactatgcta taagagatat atggtcgcct gtatggattg aaaagaagat
     2101 tatcactcct cagtttgacg gaaggctgaa aatcgaaaac aggtttcatt acacgtcact
     2161 gtcacagtgc agtttcagtt attaccttgt aaagctttct gattcgtttt atccatcaga
     2221 agcttacagg atctcaagaa cggcttcccc tccatctctg ctgcccgggg aaacaggctg
     2281 gctcaatctt aaccttcccg acaactggcg tgactttgat gtgatatatc tgaatgcaac
     2341 cgatccccac ggaaggctaa tcaacacatg gtcgtggaat atctcttcac cggctgaaat
     2401 tgcttcacgt atcgttaaac cggagggagc taaagcagaa ggaaaggatg aaggagaaag
     2461 agtcataatt gcttccggag atgtaagggt gtcaataaac aagtccacag gtcttattga
     2521 agaggtaaga aaagatcagg aaataattcc cttcaccaac gggccgcggt ttacagggct
     2581 aaatccttcg ttcagggaac taaagcatta ttcttccggt gataattatg ttgtggagtt
     2641 aatttacagc aacaggcctg aatgtcatgt aaaatacacc atgatgccgg gagggtggct
     2701 gaagcttgaa tgggattaca aaccaacagg gacggccgat cttttcggta ttaccttttc
     2761 atttcctgag gaacaggtta caggagcgat actggaagca aacggaccct atcatgtatg
     2821 gaagaaccgg ttgagaggaa cactttttgg tatatacgat aaaaaataca acaacacggt
     2881 aacgggtgaa tcgtgggatt atcctgagtt taaaggatat tattccaact tttaccaggt
     2941 gaaagtcctt acaaagaatc ttcctttcac tgtggtttca gccactgaag atctttacct
     3001 gcatcttttt acacctcaga agcccaggca tgcggcagga tatgttacgc ctccatttcc
     3061 tgaaggggat atttcattcc ttaatggtat cagtgcgatt ggaacaaaat tcctgtcacc
     3121 tgaacagctg ggtccacaga gtcagaagaa tatttacctg ctcaacagga atacggagcc
     3181 gctggcagga tgtgtgtaca ttaaatttgg ggaatgaaat ttgaagggca cagggcgcac
     3241 gaaacgaagc ggagtcccgc atagcgggag agcacactaa gcgaaggata catgtattcc
     3301 ctgcccgaat gacataatca ggtgggggta atcggtaatc tgtaatcggt gatagtatac
     3361 actgttaaaa aaccttaatc tggtcaaatt attatgatat tattgacaaa aagaatattt
     3421 cgtttgattt caatttctta tttttaaagt tctgaatcta acctgaaaaa attctttgaa
     3481 atgaaaacat ctgtattttt tgtttcgctg gcattaatgc tatcggttat ttcctgcaat
     3541 acttcagata aaaaggctgg cactgctgcc agagttgatg tttctgaatt tctcgggcag
     3601 tggacactcg acattgaagg tggcactgtg ggctggcttg aagtcaggca ggaagaaggt
     3661 taccttgatg ccgacctcct ctggatagcc ggcagtgtga caccggttgc gaatgtcttt
     3721 cttgccgcca accaatacct tgtggtaaca cgcacaaaca acctgatacg caagcgtgat
     3781 gaagcaggtc agccactgag aacacacgtc gtaaccaact ggcttgaagt acggaaagac
     3841 ggggcagaaa aactaaccgg ttatcttctc accccccgcc gcaatggtct gggtgttgat
     3901 tcgacatcct ttaccggtaa aaaacttcct cctgtgcctc ctgcacccga cctttcaaaa
     3961 gtaaaatacg gagagcccat cgtcctgttc aatggcaaag acctgaccgg ctggcggctt
     4021 atcgaggaaa atactaccaa tggttttaag gtggtcgatg gtgtactggt taatgaccct
     4081 gtccagccag aaggccagcc aagaataagg tacggaaaca tacgcaccga acaggaattc
     4141 gaggatttca acctgaaact tgaagtcaat gtgcctgaag gcagcaacag cggtatatac
     4201 ctgcgtggaa tgtatgaagt ccaggtcatg gattcataca aa
//
LOCUS       DAOSNB010000226         4240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20935_length_4240_cov_4.956511, whole genome shotgun sequence.
ACCESSION   DAOSNB010000226 DAOSNB010000000
VERSION     DAOSNB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20935_length_4240_cov_4.956511"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLG42_08855"
     CDS             complement(<1..796)
                     /locus_tag="PLG42_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU31356.1"
                     /translation="MLVYTIKELCRTCYTCVRECPAKAIRIAGGQAEVIADRCIACGN
                     CTKVCSQGAKVFLKTTDRVETLLRKNDRTAAIVAPSFPAEFSEISDHRILVGMIRALG
                     FSIVAEVSFGADLVAQRFRKLIEEGNKYYITSDCPSIVNYVRYYHPSLVDRLAPIVSP
                     MVAASKVIRRKYGSDLKVVFIGPCIAKKAENSEIDEAITFVELRELFEQKGINQENTM
                     PSDFDPPEGGRGAIFPVTRGLLQTADIDDDAVNGNIIAAEGRIDFQS"
     gene            complement(818..2539)
                     /locus_tag="PLG42_08860"
     CDS             complement(818..2539)
                     /locus_tag="PLG42_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU31357.1"
                     /translation="MLFSITVNNRKITAEKGETILSALNRNGINIPTLCRMKDFTPTG
                     ACRLCVVEVEGRENLVTACSEPVEEWMNIRTHSPRVIKARKTIVELLLSKHPDECLFC
                     INNLDCELQRLAAELNIRERRIKGRKIKPGIDQSGHSIVKELSKCVLCGRCVRVCEEL
                     VTVSTLDFINRGQKTRVGTSMNQDLNFTSCINCGQCVNVCPTGALHVKQNITEVQEYL
                     SNHDYLKVVQYSPMVPLAVAEELGIKAPKDIDRILNGILKKIGFNKVFLTGTGTDIMI
                     EALAEEILKRTDATDQSPLFVSACPAWVKYAEQSLHDELENLSKLKPPQQITGSLIKT
                     ILSKQLGIKPQQIYSVSVTPCTAMKFEARRDSMMTKGICDVDSVLTVRELVKLIRLYG
                     IDVSGTEGEAADEPLNVRSALSVLAEVSGGITESIVRKIFFRLTGKDADRKMFRNIRS
                     GGSFREAEFAPGGRKIAVAVVDGLSGLPRLYQSIASGRKYHIVEVMVCPGGCVNGAGL
                     QVCMSKEELKNRVKQIYQTEESDILQIETFSRHLADFRGKYLDKDELLKDKKILHTYF
                     SKRDVLL"
     gene            complement(2548..>4240)
                     /locus_tag="PLG42_08865"
     CDS             complement(2548..>4240)
                     /locus_tag="PLG42_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOU31358.1"
                     /translation="PLNFEPFICIQLPGKNKLFFRNITEEKVEPLLDGVFHNDIPEED
                     IVGQKGSKDYGMWPGIPFIDEITFFAGQKRIVLGNCGCYDPESIEEYIARGGYRTFIK
                     TIRNYTHSEVCDIIERSGLRGRSGGGYLTGAKWKSALNTSASSRYLICNAKESDPGAF
                     VATTILESDPHRLIEGIAIAAYAIDATTAIIYLKAGTGIARKRITKALEQAREYGITG
                     HNIFSSGFNLDIQIRTDPGAFVCGEETALIASLEGKRGMPRLKPPYPANYGLAGKPTV
                     INNVETLMNVALIMENGPGWFRSIGTEKSKGTKIFSVSGKGRYTGVVEVEMGTTIRYI
                     VEKIADGIRDGGQFKAVEIGGASGYFIDEASLDTAIDYEVMKEKGIGMGAGGFVIIDT
                     STCIVDFVKYYMEFIHNESCGKCIPCREGTARMFEILESVIRKPEREDSFTTLERFKG
                     VMQLESIASVMKDASLCGLGQTAPNPFLSALEKFRDEFEEHIFDRKCRANVCRGLRTF
                     VIDVEKCTGCTVCATRCPQNAIYGTRLQPFFIVEEKCSGCGICYEVCKFGAVIIK"
BASE COUNT         1072 a         1042 c          816 g         1310 t
ORIGIN      
        1 cactctgaaa gtcaattctt ccttcggctg caataatatt cccatttaca gcatcatcgt
       61 cgatatcggc agtttgaaga agtccccttg ttacagggaa aatagctcct cgtccacctt
      121 caggtggatc aaaatctgaa ggcatggtat tctcctgatt aatacctttc tgttcaaaca
      181 gttccctgag ctcgacaaaa gttattgctt catcaatctc tgaattctca gctttcttcg
      241 ctatacatgg accaataaat actaccttta aatcactgcc atattttctt ctgattactt
      301 ttgaggcggc aaccataggt gaaacgattg gggcgagccg gtcaacaagt gatgggtggt
      361 aatatcttac gtagttaaca atggatggac agtcggatgt tatataatat ttgtttcctt
      421 cttcaataag ttttctgaaa cgttgggcca caaggtcggc tccgaatgac acttctgcaa
      481 caatgctgaa accgagtgcc ctgatcatcc caacaagtat tctgtgatcg gatatttcag
      541 aaaactcagc agggaagctt ggagccacta ttgctgctgt cctgtcgttt ttcctaagaa
      601 gtgtttctac cctgtcggta gtcttaagaa acacctttgc tccctggctg cacactttgg
      661 tacagttgcc gcaagctata cacctgtcgg caataacttc ggcctgtcct cccgctatcc
      721 tgatagcttt tgcagggcac tccctgacac aggtgtaaca tgtacggcag agttctttta
      781 tggtataaac aagcatgatc ttttctttag taaaacatta caacagaaca tctcttttac
      841 tgaaataagt gtgcagaatt tttttatcct tcaatagttc atctttatca agatattttc
      901 cacggaaatc tgccagatgt ctggaaaacg tctcaatctg aaggatgtcg gactcttcag
      961 tctggtaaat ctgttttacg cggtttttaa gctcttcttt cgacatgcac acctgcagtc
     1021 cggccccatt cacacagcca cccgggcaaa ccattacctc aacaatgtga tacttcctgc
     1081 ccgatgcaat tgactggtaa agacgtggca ggcctgagag tccatctaca actgcaactg
     1141 caattttcct gccgccggga gcaaattctg cttccctgaa agagccaccg cttctgatgt
     1201 tacggaacat cttcctatcg gcatccttgc cagtgagtct gaagaatatt ttcctgacta
     1261 tgctctcggt aattcctccg gaaacctccg caagaaccga cagagcgctc cggacgttga
     1321 gcggttcgtc agcagcttcg ccttcagtac ctgaaacatc tattccgtac agtctgatca
     1381 gtttcaccaa ttctctgaca gtcaaaacgg aatcaacatc acaaattccc tttgtcatca
     1441 ttgaatcgcg gcgtgcttcg aacttcatcg ccgtacacgg cgtaaccgac acggaatata
     1501 tctgttgtgg tttgatgccc aattgttttg aaagaattgt ctttataagg gatccggtaa
     1561 tctgttgggg gggcttaagt tttgaaaggt tttccagttc atcatgcaat gactgttccg
     1621 catattttac ccaggccggg caggctgaaa cgaacagagg gctttggtca gtcgcatctg
     1681 ttcttttaag tatttcttct gccagcgctt ctatcattat gtcggttccg gttcctgtaa
     1741 gaaagacctt gttgaatcct atctttttca gtatcccatt aagtatcctg tcaatatctt
     1801 ttggtgcctt gatgccgagt tcttcagcaa cagccaaagg aaccattggt gagtattgca
     1861 ctactttcag ataatcatgg ttgctgaggt attcctgaac ctcagtaata ttctgtttca
     1921 catggagggc tccggtagga cacacattca cgcactgtcc gcagtttatg caactcgtaa
     1981 aattaagatc ctgattcatt gatgtcccga cacgtgtttt ctgtcctctg tttataaagt
     2041 cgagtgtgga caccgtgact aattcttcac aaaccctgac gcaccggccg cacagaacac
     2101 atttcgaaag ctctttcact atggagtgac ctgactggtc aatgccgggt ttaatcttcc
     2161 tgccctttat tctcctttct cttatattaa gctcagcagc aagtcgctga agttcacagt
     2221 caagattatt tatacagaag aggcattcat cgggatgttt tgagagaagc agttccacta
     2281 ttgtctttcg ggctttaatc acccgcgggg aatgtgttct gatgttcatc cattcttcta
     2341 caggttcgga acaggctgta accaggtttt ccctgccttc tacctccacc acacatagcc
     2401 tgcatgcgcc tgtaggagta aaatccttca tcctgcagag ggtgggaatg tttataccgt
     2461 ttctgttaag agccgaaaga attgtctcgc ccttttctgc tgttatcttc ctgttattta
     2521 ccgttattga gaacaacata tcccttctta ttttattatc actgctccga atttgcaaac
     2581 ttcataacag atgccacagc cagagcattt ttcctcaact ataaagaatg gctgcagcct
     2641 ggttccgtaa atagcattct gaggacatct ggtggcacaa accgtacagc cagtgcattt
     2701 ttcaacatca attacaaatg ttcttaatcc cctgcataca tttgccctgc actttctgtc
     2761 gaagatatgt tcttcgaatt catctcggaa tttttcaagg gcgctgagga acgggttagg
     2821 tgctgtctga ccgagcccgc acagtgaagc atctttcatc accgatgcaa tagattcaag
     2881 ctgcattaca cccttgaagc gttcgagtgt ggtaaatgaa tcttctcttt caggctttct
     2941 tatgacactc tcaagaattt caaacattct ggctgtgcct tccctgcaag gtatacattt
     3001 accgcaactt tcattatgaa taaattccat gtaatactta acaaaatcca caatgcatgt
     3061 tgaggtatct attatgacaa atcctcctgc tcccatgcca atacctttct ccttcatcac
     3121 ttcataatct atcgctgtat cgagcgaggc ttcatcaata aaataccccg aggcacctcc
     3181 tatttcaaca gccttgaatt gaccaccatc cctgattcca tcagcaattt tttctacaat
     3241 atatctaatt gtcgtaccca tttcaacctc aactactcca gtatatcttc ccttgcccga
     3301 tacagagaag atttttgttc ccttgctttt ttcagtgcca atgcttctga accagccagg
     3361 tccattttcc attataagag ctacattcat cagagtttca acattattta tgactgttgg
     3421 tttacctgcg agtccatagt tagcagggta cgggggtttc aacctcggca tgcctctttt
     3481 cccttcaagg ctggcaataa gagcggtttc ttcaccgcaa acaaaggcac ccggatcagt
     3541 tcgtatctgg atatcaaggt taaagccact tgaaaaaatg ttatgacccg ttattccgta
     3601 ttctcttgcc tgttcaagag ctttcgtaat acgtttacgc gcaatgcctg tgcccgcttt
     3661 gagatagatg attgctgtgg tagcatcgat ggcatatgct gcaatagcta taccttctat
     3721 cagtcggtga ggatcgcttt cgagaattgt cgttgcgaca aaagccccgg ggtcactctc
     3781 ttttgcattg cagataagat atctggaaga agcactggta ttgagagcgc ttttccattt
     3841 tgcacctgtc agatagcctc cgccactgcg acctcttaaa ccgctcctct caattatatc
     3901 acaaacttct gaatgagtat aattcctgat ggttttaatg aatgtgcggt aaccgcccct
     3961 cgcaatatat tcctcaatac tttcagggtc gtagcagcca cagttgccca gaactattct
     4021 tttctgccct gcaaaaaagg taatctcatc aataaacggt attcctggcc acatcccgta
     4081 atcttttgac cccttctgtc caacaatatc ttcttcagga atgtcattgt gaaaaactcc
     4141 atcgagcagt ggttcaactt tttcttctgt tatgttcctg aaaaatagtt tattctttcc
     4201 cggaagctgg atacagataa aaggttcgaa atttaaaggt
//
LOCUS       DAOSNB010000227         4233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_20985_length_4233_cov_4.196506, whole genome shotgun sequence.
ACCESSION   DAOSNB010000227 DAOSNB010000000
VERSION     DAOSNB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20985_length_4233_cov_4.196506"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1040)
                     /locus_tag="PLG42_08870"
     CDS             complement(174..1040)
                     /locus_tag="PLG42_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOU31359.1"
                     /translation="MEEQIKEWSEKIVPWLLSHGIKIVLIAAAAWILNRILARIIDRA
                     VRIAVVRDETMTLEAERQREDTLIRIFTGAMSIVIIIIAVLMILKEAGLDIGPMLAGA
                     GIVGLAVGFGGQYLIRDIITGLFIILENQYRIGDVININDKSGVVEDISLRKTTLRDI
                     NGTVHHIPHGEIKIVSNQSKDFARVNIDIGIAYNSNLEKVIRVVNQTGEELARDPYFK
                     ELIINPPKFLRVNEFADSAIIIKILGDTKPLKQWEVAGELRKRLKIAFDREGIEIPFP
                     QRVIHNISEKKE"
     gene            1170..2063
                     /locus_tag="PLG42_08875"
     CDS             1170..2063
                     /locus_tag="PLG42_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31360.1"
                     /translation="MAGDSISSRSCLSVLLLLTFYVSAVSQEPGISQQNEKSGDNEPI
                     HSFYGGVTYGNNMIYLGTNLSEDKPFYSGTLIYGYKNKVFLSASASHLTAFDPLVSFS
                     SVAGTFTHNLNSWFDVSGGVSGFLVNKNLTDTLFSNFVYGNISLGLDWRILYTNISAG
                     GVLSESGSFYLNIKNSRYFETPSFMKGKAYFSFEPYVNLMFGKLTKTTTSEGTFIGVS
                     APFKPSRQSGQHSSGTSVITTTYFSVIEADFGLPVTFNAGNFSLEVEPGYVLPAYNTS
                     SSDVQSPEGFTLLVTLFYRIF"
     gene            2163..2597
                     /locus_tag="PLG42_08880"
     CDS             2163..2597
                     /locus_tag="PLG42_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31361.1"
                     /translation="MKTRIFMTVMALIISGTIMAQNTQQNRAQTKTQTQVQTQESVKT
                     QTQTQAGTMTQTRNQQMGGVQTRSEVRNQEQLSKGEKQARAQERKELKKQQKELKKQQ
                     KEQNREEYREQEETATMNKGARSATPQRNAVKTARSSGTGRK"
     gene            2762..3802
                     /locus_tag="PLG42_08885"
     CDS             2762..3802
                     /locus_tag="PLG42_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31362.1"
                     /translation="MKTGNIEIPYNSRGIILVTNFLILFSFAGYSQTDEITSISDTTG
                     KEGDKSRSILYTGGGYGSNMIYLGSSISGSKPYKYYFLSCNITKSLSISASGFHISDL
                     SPIFSYYNFSLNYSRVFNSWFDISSGVCRYQVNSSLPDTFMTSFNYGDFTAGFDWTIL
                     YTQLSAGVFLTGKPQVYFQLKNSRYFNILSFSGGKTSISVNPYVSALLGNLITAETYS
                     GEYVVTTTHEYNNPAAPSAVTGKTSPGYGSGGGYGSGYGGGTGQSTSSGTTTTTTTTV
                     TTTTSVPYTETFYKKNFGLIDAEIGLPVSFNTERFIFEAELNYVLPAYNYPGFRFKDG
                     VVFMISGIIKIF"
BASE COUNT         1253 a          820 c          838 g         1322 t
ORIGIN      
        1 tgcccacaat gtttcttctg aaaggctcga aatatttttc aagatcagtc ataatgaatc
       61 tttttaaatc agccggatac ctgcaatttt atattagggc gaaaaaatgt ttttaagtca
      121 aattgaaaaa gggttttatt gcgttcgtaa tttattcaaa gaaaggtgga tttttactct
      181 tttttctcag atatattatg aattaccctt tgcgggaagg ggatctctat tccttccctg
      241 tcgaaagcta ttttcagccg tttgcggagt tctcctgcca cttcccattg tttcaggggc
      301 tttgtgtctc ccaggatttt tatgattatt gccgaatcgg caaattcatt gactctcaga
      361 aatttgggcg gattgataat cagttcctta aagtatggat ctcttgcaag ttcttcgcct
      421 gtctggttaa ctacccgtat tactttttca agatttgagt tgtaagcaat gccaatgtcg
      481 atatttactc ttgcaaagtc tttcgactgg tttgaaacaa tctttatttc cccatggggt
      541 atgtggtgga ctgtgccgtt aatgtcgcgc agtgttgttt ttctgaggct tatatcttca
      601 acaacaccgc ttttgtcgtt gatatttatt acatcaccaa tgcggtactg attttcgagg
      661 atgataaaga gacctgtaat tatgtcgcgt atcaggtact gacctccgaa tccaacagcc
      721 agacccacaa tacctgcgcc ggcaagcatg ggtccgatat ccagtccagc ttccttcaat
      781 atcatcagca cagcaattat tattattact atgctcatgg ctccggtgaa aatacgaatc
      841 agggtgtctt ctctctgtct ttctgcctct agggtcatag tctcatcgcg aaccactgca
      901 attctaacag ccctgtcaat tattctggca agaatccggt tgagtatcca tgctgctgca
      961 gcaatcagaa ctatttttat tccgtgcgaa agtaaccagg gaacaatttt ttccgaccat
     1021 tcttttattt gttcttccat attatctgaa taaacagttt taaacaacca tccgggtgcc
     1081 tcaaaggtat aaaacatata agaaaatttc atgatttctt catctggtta ccaatagttt
     1141 tgttaaagtt gaactaaaat cttttaaata tggcaggtga tagtatttca tcaaggtctt
     1201 gtctgtcagt actattgtta ctgacatttt acgtttcagc tgtatctcag gaaccaggaa
     1261 tttcacagca aaacgaaaag tctggcgata atgaacccat acattctttt tacggaggag
     1321 tgacatatgg taataacatg atttatctgg gaactaatct gtcggaggat aaaccatttt
     1381 attcgggtac tctgatatat ggttataaaa ataaagtttt tctttcggcc tcggcaagcc
     1441 atttgacggc atttgatccg ctggtttctt tttcatcggt tgcgggaact tttacacata
     1501 atttgaattc ctggtttgat gtttcaggcg gagtatccgg gtttcttgta aacaagaatc
     1561 tgaccgacac attattcagc aattttgtat atggtaatat ttcattaggg cttgattgga
     1621 ggattctata tacaaatatt tcagccggag gagtgttatc tgaatcgggc agtttttatt
     1681 taaatatcaa aaactcgagg tatttcgaaa ctccttcatt tatgaaaggc aaggcatatt
     1741 tttcatttga gccctatgtt aaccttatgt ttgggaagct aacaaaaact actacatcag
     1801 aaggaacctt cataggagta tcggctcctt tcaaaccttc caggcaatcg ggtcagcatt
     1861 cgtcaggcac ttctgttata actacaacgt atttcagtgt aattgaagca gattttgggc
     1921 ttcctgtaac atttaatgcc ggcaattttt ccctggaagt tgaacccgga tatgtattgc
     1981 cagcttataa caccagcagc agcgatgtcc agagtcctga aggttttacc ttacttgtaa
     2041 ctctttttta taggatattt tagaattatc aggtacctgt aaaaaatctt ttaatttttc
     2101 atcatatctt cattttcaga tacttagatt tgatatgaaa ttaaccttaa aattttagaa
     2161 cgatgaaaac aagaatcttc atgacagtaa tggctcttat aatcagcgga acaattatgg
     2221 cccagaacac acagcaaaac agagcccaga caaagacaca gactcaggtt cagactcagg
     2281 agtctgtaaa gacccagact cagacgcaag caggaacaat gacccaaacc cgtaaccagc
     2341 agatgggagg tgttcagaca cggtctgaag taagaaatca ggaacagtta tcaaagggtg
     2401 agaagcaggc aagagcccag gagaggaaag aattgaagaa gcagcaaaag gaactgaaga
     2461 agcagcagaa ggagcaaaac cgtgaagagt acagagagca ggaagagacc gctacgatga
     2521 acaagggtgc caggagtgca actcctcaga ggaatgcggt taaaactgca agaagttcag
     2581 gtacaggccg gaaatagttc agaattttta aaattcaaaa aagccctgat ttcaaatcga
     2641 ttcagggctt ttttatttag caggtggttc acatggtgct gaattcattt catcaaatct
     2701 tcatttttaa atatttaaat tcggaataat tggatcctgc tggactattt gatattatat
     2761 catgaaaaca ggaaatattg aaataccata taatagtagg ggtattatat tagtgactaa
     2821 ctttctgatt ttgttttctt ttgctggata ttctcaaact gatgaaataa cttcaatatc
     2881 cgatactaca ggaaaggagg gggacaagtc cagaagcatt ttgtatacag gtggaggtta
     2941 tggaagcaac atgatctatc tgggatcctc aatctccgga agcaaacctt ataaatatta
     3001 ttttctttca tgcaatatta caaagtcgct gtcaatttca gcatcaggtt ttcatatttc
     3061 ggatttaagt ccaatttttt cgtattataa tttttccttg aattacagtc gtgtttttaa
     3121 ttcatggttt gacatttcat caggagtttg caggtatcag gttaacagtt ccctgcccga
     3181 tactttcatg acaagtttca attacgggga ttttactgca gggtttgact ggacaattct
     3241 ttatacccag ctatcggccg gagttttttt aacaggtaag ccccaagtat atttccagct
     3301 taaaaactcg agatatttta atattttaag tttctccgga gggaaaacat caatttctgt
     3361 taacccttat gtatcagcac ttttagggaa tcttatcact gctgagacat attcagggga
     3421 atatgtggtt actactactc atgaatataa caatccggct gcaccgtctg cagtaacagg
     3481 caaaacttct ccgggttatg gttccggagg tggatatggt tcgggttatg gcggtggcac
     3541 agggcaatca accagcagcg gcacaacaac taccactaca actacagtaa ctactacaac
     3601 ttcggtgccc tatactgaaa cattctacaa aaaaaatttc ggacttattg atgctgagat
     3661 cggccttccg gtttctttta atactgaaag attcattttc gaggcagaat taaattatgt
     3721 attacctgcc tacaattacc cgggatttcg tttcaaagac ggagttgtat ttatgataag
     3781 cgggataatt aagatcttct gaacgatacc aaccccctcc ctacttcgca aagagggggc
     3841 ttacaaccaa atatatcgtc ctgaaaaaag ttgacagaga aatgatatat tttccagttt
     3901 ctttatattc aatgaccccc tttcccgttt cccccaaggg ggaaaagctt aacaaattga
     3961 tacatagaat tctgtcttta attttttccc ccgtggggag aaatacgtag gggggtcagt
     4021 ttttatacaa ggaaatacaa aggcgggtca gtctcacatg aactaccgga atacatgtat
     4081 ttcggagtcc cgctttgcgg gataaattcc acgaagatcc caataacgaa gcgtatcgga
     4141 aacaattgaa ttactattga attactatta aattacaatt aagcccccct gccagggggg
     4201 ttggggggtc aacaatacta tgcccctgcc agg
//
LOCUS       DAOSNB010000228         4230 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21006_length_4230_cov_3.763832, whole genome shotgun sequence.
ACCESSION   DAOSNB010000228 DAOSNB010000000
VERSION     DAOSNB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21006_length_4230_cov_3.763832"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="PLG42_08890"
     CDS             <1..1447
                     /locus_tag="PLG42_08890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU31363.1"
                     /translation="IFAAGKAIKYTSTDNYCMSCHIHPHAEQTWRLSTHYNNKSGTIV
                     HCVECHLAPEGKGYLFTKAKHGLKDLYGYWFKDSASINWEAKRLVENAKNFVYEKSCL
                     ECHQNLFPVTLSKEGDDAHLFYTLSENPPRCINCHLTVGHFDKNFRHAKDTTFGVIKA
                     ADALIYKEPAKVEKFEDFTETIPGTNVSFRMKAIPGGKYMMGSPDDEPLRDNDEGPVR
                     EVTVSPFWIGEVEVTWDEYLAFFQATSSQGRTEGQVVTTGKVDAISGPTPPWGAPDQG
                     WGKGSRPAITMTWYAANVYCKWLSKVTGKKYRLPTEAEWEYACRGGTSTPYFFEGDPK
                     KFTSEGFLKKIFKPDTSVISSYVIYKVNSNFRTAEPSAVRPNPFGLRNMLGNVAEFCL
                     DYYSPDTYRTDTVRVNPRGPEWGEEHVIRGGSYKSDARDVRCASRDFTKTKAWLVTDP
                     QMPKSIWWYSDCIDVGFRVVCEPDSSILNKR"
     gene            1458..2795
                     /locus_tag="PLG42_08895"
     CDS             1458..2795
                     /locus_tag="PLG42_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31364.1"
                     /translation="MKDIGRRKFIGRAALAGIGMAAAASISGGCKRKEILPVEGLPPV
                     LKAAPKGRKLKAGLVGAGGRGTGAAINFISAGPDLEIVAVADVFQDKVDAFCKRFESF
                     GLPIPPENRFTGFDGYKKLMAIDDIDVVILATPPKFRPEHFLEAVTMKKHVFMEKPVA
                     VDSNGIRTILATAKKAEALGLNVVTGTQRRHQEDYIATYKKVAEGAIGRIVAAKAWWN
                     QDHVWFRLREPGWNDMEYMIRNWNNFIWLSGDHILDTHVHNIDVVNWFTGKHPVSAIG
                     YGGRHRRVTGDQYDFFSIDFDYGNGVFSHSMCRQIDGCANGTGELVVGTEGYTNCQNT
                     IWNLDGKVIWKFEYPKDENGNSTNSVAVSPYIQEHMHLVWAIRTGNYVNEAEQTAIST
                     LTAIMGRTAAYTGKMISWDEILKMDMNLGPSKIEFGPVDMKFEPPVPGLPVQI"
     gene            2829..4142
                     /locus_tag="PLG42_08900"
     CDS             2829..4142
                     /locus_tag="PLG42_08900"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31365.1"
                     /translation="MSMKSSRRKFLKKAASAVTGTLVFPYIIPSSSLGLGGETPPSEK
                     ILMGVIGTGSQGMSNMRSFMRLKDQVRFVALCDVDAKRLEAAKAVVDKVNRSNDCRIY
                     GDFREFLEKEKLDAVLIAIPDHWHGIIYTAAANKKLDVYGEKPICRTINDGKVIVQAV
                     KKNGIIWQTGSWQRSEANFHRGAELVINGRIGKVKYIEVGLPDGVRGIGTPPVQEVPE
                     GLDWEMWLGPALKVPYRGVCHWNWRWILDYSGGQLTDWAGHHVDIANWGTGLEYTGPV
                     EIEGTGFYPYDGIYNAPVEYDITCKFANGIIMRVANASRLPKGMGTAWYGDLGWIHVD
                     RGNRISASDPKILEETIGENEIHLYKSTDHFQNFIDCVRTRKEAVAPIEPAYRAISTA
                     LLGEIAMTTGQKIYWDPEKEEIIGNPTASRLLGRPYRKPWTLPEV"
BASE COUNT         1297 a          830 c         1079 g         1024 t
ORIGIN      
        1 gatttttgct gcaggcaaag caataaaata tacttcaacc gataattatt gcatgtcgtg
       61 tcacatacat ccgcatgctg aacagacatg gagactatcg acacattaca ataacaaatc
      121 cgggactatt gttcattgtg ttgaatgtca tctcgctcct gaaggcaaag gttatctgtt
      181 tacaaaagcg aagcatggat taaaagacct gtacggttac tggttcaaag acagtgcatc
      241 tataaactgg gaggcaaaga gacttgtaga gaatgctaaa aattttgtat atgaaaaatc
      301 gtgtctcgaa tgtcatcaga atcttttccc tgttacacta tcaaaagaag gggatgatgc
      361 tcacctgttt tatactctca gtgaaaatcc tccccggtgt ataaactgtc accttacagt
      421 agggcatttt gacaaaaatt tcaggcatgc aaaggataca acctttggag ttataaaggc
      481 cgctgacgca ctaatttaca aagaaccggc gaaggtcgaa aaatttgagg atttcactga
      541 aaccataccg ggtaccaatg tttctttcag aatgaaagca ataccgggag ggaaatatat
      601 gatgggtagc cccgacgatg aaccgctgag agacaatgat gaaggaccgg tgagagaagt
      661 tactgtgtcg ccattctgga ttggagaggt ggaggtgaca tgggatgagt atctggcatt
      721 tttccaggct acctcatcac agggcaggac agaagggcag gtggtaacca cagggaaggt
      781 agatgcaatt tcgggaccca caccgccatg gggtgctcct gaccaggggt gggggaaggg
      841 ctcacggcct gcaataacta tgacatggta tgcagcaaat gtttactgca agtggctttc
      901 aaaagtaacg ggtaagaagt acaggctgcc taccgaagct gaatgggaat atgcatgcag
      961 gggcggcacc tcaactccat atttcttcga aggcgacccg aaaaagttta cttcggaagg
     1021 attcctgaag aagatcttca agcccgatac gtctgtaatc tcttcgtatg taatatataa
     1081 agtcaacagt aatttcagaa ctgcagaacc ttcggctgtc agacctaatc cgttcgggtt
     1141 aagaaatatg ctgggtaatg tggctgagtt ctgtctcgat tattattcac cggatacata
     1201 ccggaccgat acagtaagag ttaacccgag aggtccggaa tggggcgagg aacacgtcat
     1261 aagaggtggt tcatacaaga gtgatgcacg ggatgtaagg tgtgcttcgc gcgactttac
     1321 aaaaacaaaa gcatggctgg taaccgaccc gcagatgcct aaaagcatct ggtggtattc
     1381 cgattgtatt gatgtgggat tcagagtagt atgtgaacct gacagttcaa tattaaataa
     1441 gagataataa attaaaaatg aaagatatag gacgaagaaa atttatcgga agagctgctc
     1501 tggcaggaat agggatggca gcagcagcat ctatttctgg cggttgcaaa agaaaggaaa
     1561 tattacctgt ggaagggctt ccgcccgtac tgaaagctgc acctaaaggc aggaaactta
     1621 aagcgggtct ggtaggtgcc ggaggaagag gcacaggtgc agccattaat ttcataagtg
     1681 cagggcccga cctcgaaatc gttgcagttg cagatgtgtt tcaggataag gtggatgcct
     1741 tctgcaaaag attcgaatcg ttcggtctgc caataccccc tgaaaaccgt tttacaggct
     1801 tcgacggtta caaaaagctt atggctattg acgacattga cgtagtcatt cttgccactc
     1861 cgcccaagtt caggccggaa catttcctcg aagccgtaac gatgaagaag cacgttttca
     1921 tggagaaacc ggttgcggtt gattcgaatg gaataagaac tattcttgcc acagcaaaaa
     1981 aggctgaagc cctgggactt aatgtcgtaa ccggaaccca gcgacgccac caggaagact
     2041 atattgcgac atacaaaaag gttgctgagg gagcaatcgg acgtattgtt gctgcaaaag
     2101 catggtggaa ccaggatcat gtttggttca ggctccggga accgggctgg aatgacatgg
     2161 aatatatgat acgaaactgg aataacttca tctggctttc aggcgaccat atactcgata
     2221 ctcatgtcca caatattgat gtagtaaact ggttcaccgg aaaacaccct gtaagtgcaa
     2281 taggctatgg cggcagacat agaagagtaa caggtgatca gtacgatttt ttcagtatag
     2341 attttgatta cggaaacgga gtcttttcac atagcatgtg ccggcagatc gacggttgtg
     2401 caaacggaac cggcgaactg gtagtgggta ctgaaggcta tacaaattgc cagaatacga
     2461 tctggaacct cgacggaaaa gtaatctgga agtttgagta tccgaaagac gaaaacggaa
     2521 actcaacaaa ctcggtggct gtttcgcctt atattcagga acatatgcat ctggtatggg
     2581 caatccgtac cggtaattat gtcaacgagg ctgaacaaac tgcaatttca actctcactg
     2641 ctattatggg cagaacggca gcatataccg gcaagatgat ctcctgggat gaaatactga
     2701 aaatggatat gaatctcgga ccatcaaaga ttgaattcgg tcctgtcgat atgaaatttg
     2761 aaccacctgt gcccggtttg ccggtgcaaa tctgaaaatg attatattgg atacaaaatt
     2821 taaaccaaat gtcaatgaaa agctcgagaa gaaagtttct gaaaaaagct gcttcggcag
     2881 ttacaggaac tttagtgttt ccgtatatta ttccttcatc atcacttggg cttggaggag
     2941 agactccgcc aagtgaaaaa attcttatgg gggtaatagg tactggttcg cagggtatga
     3001 gcaacatgag gagttttatg aggctgaaag atcaggtacg ctttgttgcc ctctgtgatg
     3061 ttgacgcaaa acgtctggaa gcagcaaagg ctgttgttga taaggtgaat cgcagcaatg
     3121 actgccgtat atacggtgat ttcagggagt ttctggaaaa ggaaaaactt gatgcagttc
     3181 tgattgctat acctgatcac tggcatggaa taatttatac agcggcagct aacaagaaat
     3241 tggatgttta cggagagaaa ccaatttgtc gcaccatcaa tgatgggaag gtgattgtgc
     3301 aggcagttaa gaaaaacgga attatttggc agacaggaag ctggcagagg tcggaggcaa
     3361 actttcaccg tggtgcggaa cttgtaatca acggacgaat tggtaaagtc aaatatattg
     3421 aggtgggatt gcccgatggt gtaagaggga taggcacacc tccagttcag gaggttcccg
     3481 aaggtctcga ctgggagatg tggctgggcc cggcactgaa ggttccttac cgcggagtat
     3541 gccactggaa ctggcggtgg attctcgatt actctggagg gcagcttact gactgggcgg
     3601 ggcaccatgt cgacattgct aactggggca ccggacttga atacacaggc cctgttgaaa
     3661 tcgaagggac aggcttttat ccttatgatg ggatttataa cgctcctgtc gaatatgaca
     3721 ttacatgcaa gtttgcaaat ggaataataa tgagggtagc caatgcatca cggcttccta
     3781 aagggatggg aaccgcctgg tacggcgatc ttggctggat acatgttgac aggggcaata
     3841 gaataagcgc atccgacccc aaaatacttg aggaaacaat cggcgagaat gagattcatc
     3901 tttataaaag cactgatcat tttcagaatt ttatcgactg cgtacgtaca cgcaaagaag
     3961 cagtagcacc tatagaacca gcttaccggg ctatatctac agccctgctc ggtgaaattg
     4021 caatgacaac aggacagaaa atatactggg atcccgaaaa ggaagagatc ataggaaatc
     4081 ccactgccag ccgactgctc ggaaggcctt accgtaaacc ctggacatta ccagaagtat
     4141 aaaacgatga gatatgaaaa ggatattaaa tatattattg attatcagca taattctgtt
     4201 aactcttaca gaatgtaaaa aaagcccgca
//
LOCUS       DAOSNB010000229         4210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21135_length_4210_cov_3.639230, whole genome shotgun sequence.
ACCESSION   DAOSNB010000229 DAOSNB010000000
VERSION     DAOSNB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21135_length_4210_cov_3.639230"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLG42_08905"
     CDS             complement(<1..543)
                     /locus_tag="PLG42_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU31366.1"
                     /translation="MKKFAFILSASILTTSIINSQQIKTPEQFLGYEPGTQFTYHHKA
                     VEYFKYVADSSPLAEYWSYGESYEGRPLGVCIVSSEENLKNLDELRKNNLVKTGLHKG
                     EYTGKQVPFIWLAYNVHGNEAAGMETAMKVLYTLVSGDYPGVKDWLNACVIIIDPCQN
                     PDGREMYTARYRSSQNLSPNP"
     gene            719..2290
                     /locus_tag="PLG42_08910"
     CDS             719..2290
                     /locus_tag="PLG42_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HOU31367.1"
                     /translation="MNFRNIVILSFLVFSILPSSVLNSQDQAHNPVIWADVPDMSMVR
                     VGDTYYMTSTTMHMSPGVPIMKSRDLVNWEIVNYAYDILDNVDALNLENGKNAYGRGS
                     WASCIRYHDGTFYVSTFSQTTNKTYIYLTKNIEKGPWKVHSFSPALHDHTLFFDDDGR
                     VYMIYGNRRLTLVELNADLSGIKAGGVNQVIIEDSSIPSGSGSGLGEGSQLFKINDKF
                     YLFNITWPKGSMRTVVVHRADRITGPWEGKMVLQDKGVAQGGLIDTPDGRWFAYLFRD
                     YGSVGRIPYLVPVKWENDWPVLGIDGKVPDILELPASRGLIPGIVAPDEFGRRKGEPL
                     LPLVWQWNHNPDNSLWSVTERKGWLRLRTGRVDTLFLMARNTLTQRTFGPVCSGSTCL
                     DVSGMKEGDFAGLALLQRKFGQVGVKYENGAKYVVMVNAQSGKPVEIERVPFIGKKVY
                     LKTECDFRNLADVAWFYYSTDGKTWKSIGNQLKMEYTMPHFMGYRFGLFNYATRSPGG
                     YADFDWFRVSKEIIQ"
     gene            2312..3661
                     /locus_tag="PLG42_08915"
     CDS             2312..3661
                     /locus_tag="PLG42_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOU31368.1"
                     /translation="MKKFILISFIFYVLTSGLFIGLYAQNPIIRNQFTADPTARVFNG
                     KVYLFPSHDIPAPERFPRKDWFCMEDYHVFSSSNLTEWTDNGVIVSQYNVNWVDTNSF
                     SMWAPDCVERNGKYYFYFPANVNAKYGKGFGIGVAVADKPEGPYVPQPEPIKGVRGID
                     PCVLIDEDGQAYLYYSLNRIFVAKLKENMLELDSEPVVIANLPAKGLIEGPFVFVRNG
                     IYYLTYPHVENKTERLEYAIADSPMGPFKVAGVIMDESPTGCWTNHHSIVEYNGQWYL
                     FYHHNDYSPKFDKNRSARIDSLFFNADGTIRKVVPTLRGVGLTSARDRIEIDRYSQIS
                     DKGVSLAFIDTLNTFKGWKIRLGMQNAWVKYNSVDFGNKKLKSVNVRVFSLSGSVLQI
                     KVDSADGLLLGEVKIPSGVEWTTVRSRIKNSPKGVHHLVVILKGDNPVEVDWVQFGD"
BASE COUNT         1203 a          736 c         1020 g         1251 t
ORIGIN      
        1 gggattggga gagaggttct gcgacgatct gtaacgggca gtgtacatct cgcgaccgtc
       61 gggattctgg caggggtcaa taattataac acatgcattg agccaatcct tcacacctgg
      121 ataatcgcct gaaacaagtg tatataaaac tttcattgcc gtttccattc cggcagcttc
      181 attgccatga acattgtatg ccagccatat aaaaggcacc tgtttgcctg tgtattctcc
      241 tttatgaagg ccagttttta caagattgtt tttccgcagt tcatcaaggt ttttaaggtt
      301 ctcttctgat gatactatgc atacccccag tggacgaccc tcataacttt caccgtaact
      361 ccagtattcg gcaagtggag aagaatctgc cacatattta aaatattcaa ctgctttatg
      421 atgataggtg aattgcgtac caggttcata gccgagaaac tgctcaggag ttttaatctg
      481 ctgggaattt attatggaag tggtaaggat tgaagctgaa agaatgaaag caaatttttt
      541 catggttatg aaatttgaga accaaatata ttgtttttta atgtcacctg gccaattgta
      601 cttcaggatt atggtttaac aggcagcact tatttcaagg ataaaattcg tgttaaacgg
      661 tattgttcgg gtgctattaa atctgatact gattaaattt acaggaaaat actttaacat
      721 gaatttcaga aatatagtta ttctttcgtt ccttgtattc agcatcctgc catcatctgt
      781 tctcaacagc caggatcagg cacataaccc ggtaatatgg gcggatgtcc cggatatgtc
      841 aatggtcagg gtgggagata cgtactatat gacaagcacc accatgcata tgagtcctgg
      901 tgtaccaata atgaaatcac gggatcttgt caactgggag atagtgaatt atgcctacga
      961 tattctcgat aacgtcgatg ccctgaatct tgagaatggt aaaaatgctt acggaagagg
     1021 ttcgtgggca agctgcataa gatatcatga tggcactttc tatgtttcaa ccttctctca
     1081 gacgaccaat aaaacatata tatatttaac taagaatata gagaaagggc catggaaggt
     1141 acattcattc agtccggccc ttcatgacca tacgttattc ttcgatgacg acggtcgcgt
     1201 ttacatgatt tatggtaaca ggaggttgac gcttgttgag cttaatgctg atctatcagg
     1261 cataaaggcg ggcggagtga atcaggttat tattgaggat tcgagtatcc catcaggttc
     1321 tggaagcggg ttgggggagg gttctcagct atttaagata aatgacaagt tttatttgtt
     1381 taatataaca tggccaaagg gaagtatgcg cactgttgtg gttcacagag cagacaggat
     1441 aactggtcca tgggaaggca agatggtatt gcaggacaag ggagtggcgc agggcggact
     1501 gattgacaca cccgatggga gatggtttgc atatcttttc agggattatg gctctgtggg
     1561 gcgcatacct tatcttgtgc cggttaaatg ggagaacgat tggcctgtgt tgggaataga
     1621 tggtaaagtg cctgatattt tagagcttcc tgcaagcagg ggattgatac ctggcattgt
     1681 ggcaccggat gagttcggca ggcgcaaagg tgaaccactg ttacctcttg tatggcagtg
     1741 gaaccataac ccggataaca gcctgtggtc ggttactgag aggaagggat ggctgagact
     1801 tagaacaggc agggtagata cactttttct gatggcaaga aacactctca cccagaggac
     1861 attcgggcca gtgtgctcag gctcaacctg ccttgacgtg tcaggaatga aagagggtga
     1921 ttttgcagga ctggcacttc tgcagcgcaa gttcggacag gtaggggtga aatatgagaa
     1981 tggcgcaaaa tatgtcgtga tggtaaatgc tcagtcgggt aaaccggttg agattgaaag
     2041 agttccattt atagggaaaa aggtctatct gaaaacagaa tgcgatttca gaaacctggc
     2101 cgatgtagcg tggttttatt acagcaccga tggtaagacc tggaaatcca ttggaaatca
     2161 gttgaagatg gaatacacca tgcctcattt catgggttac aggttcgggt tattcaatta
     2221 tgccaccagg agtccgggtg ggtatgccga ttttgactgg ttcagggtga gtaaggaaat
     2281 aattcagtag ttaattctaa ttcagcttga tatgaaaaag tttatactta tctcatttat
     2341 cttttatgtg ctcacttctg ggctatttat tggattatat gcacaaaatc cgataataag
     2401 aaaccagttt actgccgacc ctactgcaag ggtttttaac ggaaaagttt atttgtttcc
     2461 atctcatgat attcctgcac ctgaacgttt tccgcgcaag gattggttct gtatggaaga
     2521 ctatcatgta ttttcctctt caaacctgac agaatggact gataacgggg ttattgtcag
     2581 tcagtacaat gtaaactggg ttgatactaa ctcgttcagc atgtgggctc ctgattgtgt
     2641 ggagagaaac ggtaaatatt atttctattt tccggccaat gtcaatgcaa agtatggaaa
     2701 aggtttcggc attggagttg cggttgcaga caaacctgaa ggtccatatg ttcctcaacc
     2761 tgaacctatt aaaggtgttc gaggaatcga tccctgtgta ttgattgacg aagatggaca
     2821 ggcatatctc tattattcat tgaacagaat ttttgtggca aagcttaagg agaatatgct
     2881 tgaactcgat tcagaaccgg tcgtaatagc taatctgccg gctaagggtt tgattgaagg
     2941 accatttgtt tttgtaagaa acggcatata ttatcttaca tatcctcatg ttgaaaacaa
     3001 gacagaaagg ctggagtatg ccatagcaga tagtccgatg gggcctttta aagttgctgg
     3061 tgtgataatg gacgaatctc ccacaggttg ctggacaaat catcattcaa tagttgagta
     3121 taacgggcag tggtatcttt tttatcatca taatgattat tcgccgaagt tcgacaagaa
     3181 caggtctgca agaattgatt ccctcttttt caatgctgat ggcacgatca ggaaagtggt
     3241 gccaacattg agaggtgtgg ggttgacatc ggcgcgtgac aggatagaga ttgacaggta
     3301 cagtcagatc agtgacaaag gtgtttccct ggcatttatt gatacactaa acactttcaa
     3361 gggttggaaa atcagattag ggatgcagaa tgcgtgggtg aaatacaatt cggtggattt
     3421 cgggaataaa aagctgaaat cagttaatgt aagggttttt tctctgtcag gttcggttct
     3481 tcagataaag gttgacagtg cagatggcct gcttctggga gaggtaaaga taccatcggg
     3541 cgtggagtgg acaacagtga ggagcagaat aaagaattct ccaaaaggag tacatcatct
     3601 tgtggttata ttaaaaggag acaatccggt tgaagtagac tgggttcagt ttggtgattg
     3661 attccataga tctatcagat gataatttaa ggggttttca aatcagggtt agaatacttt
     3721 attacagcag gcaggcatta atactaagag aaaaatgaat cccggcaggc tgctgcgggg
     3781 aaggggggaa ctattaagca gactgccggg atgatcgggg aaactatctt ttttattctg
     3841 tctacaacct aaatcttaat tctttttaaa atttttattt catctgtaaa aaagattaat
     3901 actatttttt attctttttt ccttaatatt atatccaaaa atgccatata aagttaaaaa
     3961 tataaattgc aaataaacat catcaataat aagtatcggg tatgtgattt tttgatgatt
     4021 ttttagtgag aattgattag ttggtattta cttttttttt gctgttccga agaatcaggt
     4081 actctcgatt cgcaggatgt cgcttcgctt cgtgcggtct gagcattgag ctctgagccc
     4141 tgtgctgggc tttaacccca aaccccctga gagggggctt gaatatcttt catttctaaa
     4201 taaatagctc
//
LOCUS       DAOSNB010000230         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21228_length_4197_cov_4.266296, whole genome shotgun sequence.
ACCESSION   DAOSNB010000230 DAOSNB010000000
VERSION     DAOSNB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21228_length_4197_cov_4.266296"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLG42_08920"
     CDS             <1..618
                     /locus_tag="PLG42_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOU31369.1"
                     /translation="RRELEQSLKNLRTDHFDLYQLHAVTTMSDVNTILGKGGALEAFL
                     QAKSEGLIRFIGMSVHSEEAGMALMEAYDFDTIMYPLHISSWHAGTFGPQLLDMAHKK
                     GMGIISLKSMAKGPWPQGADRSKYPKCWYEPFTSEEDIRMGLRFALSHPITDLMTSGQ
                     GELFKIALGLRDKLTPLSPSEVEAIKLKGAAMEPLFRYPSVESVK"
     gene            1052..2140
                     /locus_tag="PLG42_08925"
     CDS             1052..2140
                     /locus_tag="PLG42_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31370.1"
                     /translation="MRIFFLIFVLLHALIHLLGFVKGFGIREVKELTLPITKPMGLLW
                     LSATVLLVAYAISYLTHSRHAWLIGFIAAAISEILIIMFWKDARLGTLPNVIILLVSF
                     VSYGQYSSQNLVRQETEAMISQCSRISKGKIISENDIGQLPEPVKKWLNNSGVTGKSF
                     ISVAKSIQQAEMKMKPGQKKWMKASAVQYSFIDVPAFIWIADVKVNPFLSFAGRDRFI
                     DGKGEMLIKLNSLINIVYEQGEKLDEGALQRYLGEMIWFPSLALSPFITWQHIDDNTV
                     IATMSFNGTTGSGTFYFDSNGDFVKFSALRYMGNKPDSKRYEWVILVDGYKTFEGIKV
                     PSKMTATWKLEEGDWTWLKLEIIEIKYN"
     gene            complement(2372..2584)
                     /locus_tag="PLG42_08930"
     CDS             complement(2372..2584)
                     /locus_tag="PLG42_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31371.1"
                     /translation="MPCGKPAGIRCIHLTDDYRCALYGSELRPKVCIDFRPDPDFCGS
                     SREEAMKIMENLEMGRSGQPPDPMKG"
     gene            2846..3076
                     /locus_tag="PLG42_08935"
     CDS             2846..3076
                     /locus_tag="PLG42_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31372.1"
                     /translation="MKTQFLPAVFTIILMVFLIDYLSGQSAGQLATGKKFINARVTEV
                     VRVKTGIGQENMGALSPPEAEANVPMSFTFSD"
     gene            3456..>4197
                     /locus_tag="PLG42_08940"
     CDS             3456..>4197
                     /locus_tag="PLG42_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOU31373.1"
                     /translation="MKKRLIIVANRLPVKVTQIDPFTLQESEGGLATGLNSLDTNYEK
                     HWIGWPGVYAKNRKERSIILEALKKKNIHPVFLSRQQVELFYEGYCNGTLWPLFHYFY
                     VYTVYNEKYYSVYQEVNRLFLEKVLEVAREGDILWIHDYHLLILPGMIREKWKNASIG
                     FFLHIPFPSYELFRFLPERKEILRGLLGADLVGFHTYDYMRHFQSTVYRILGYEFKLN
                     EMIIDSRMVRINSFPMGIKFEKFYHSSEN"
BASE COUNT         1308 a          762 c          924 g         1203 t
ORIGIN      
        1 cgcagggaac tggagcagtc gctgaagaat cttcgtaccg accatttcga tctttaccag
       61 ttgcatgcag taacaaccat gtcggatgta aataccatac tcggcaaggg tggtgcgctg
      121 gaggcgttcc tgcaggcaaa gagcgagggg cttataaggt tcatcggtat gtcggttcat
      181 tcggaagagg ccggtatggc gctcatggag gcatatgatt ttgacacaat tatgtatcca
      241 ctgcatattt cgagctggca tgcaggcact tttggtccgc agctgctcga catggcgcat
      301 aagaagggta tggggatcat cagcctgaag tcgatggcga agggtccatg gccacaggga
      361 gccgacaggt caaaatatcc gaagtgctgg tacgaaccat ttaccagtga agaagacatc
      421 aggatgggtc tacgttttgc tctctcccac cccatcaccg acctcatgac ctcaggacag
      481 ggtgagctct ttaaaattgc tctggggctg agagataagc ttacaccgtt atcaccttca
      541 gaggtggaag ccattaaact gaaaggtgct gccatggagc cgctgttcag gtacccgtcg
      601 gtggaatcgg taaagtaaat gaggcatgag gggtgaggaa tgaggaatga ggggtgagga
      661 atgaggaatg aggggtgagg aatgagggat gaggagaaat taagtttgat ctttgtggtt
      721 tataatataa attcaacgaa aacatcctat taaattacaa taatttacaa ccaatcgtaa
      781 tgatttaatg accactgaat gacaactgaa tgacgcgccg agctaagcga ggcgcaaatg
      841 accataaaga tggcacgcgg attaacatca gtatcataaa agctcctctt tgccatagga
      901 gcctattctt cagtaaagcg aacagaatag aatataacct ttttaattac ttttacctgt
      961 tattgaagtt ttattcaggt gtttatctgg cattgccaac cgacagttta tgaagcacat
     1021 acaaaaccgt aaatagacaa tccatccgac aatgagaatt ttctttctga tttttgtttt
     1081 actgcatgca cttatccatc ttctcggatt tgtgaaagga tttggcataa gggaagtgaa
     1141 agaactgaca cttccaataa caaaacccat gggcctgttg tggctgtcgg caactgttct
     1201 gctggtggct tatgccatat catacctgac acatagcagg catgcctggc tgatcggatt
     1261 tattgccgca gccatttcag agatactaat aattatgttc tggaaagatg ccaggctcgg
     1321 aacattgccc aatgttatca ttttacttgt gtcttttgtt tcatatggtc aatacagttc
     1381 tcagaatctt gtcaggcagg aaacagaagc catgattagt cagtgcagca gaatttctaa
     1441 agggaagatt atctcagaaa acgatatagg ccagttaccg gaaccggtta aaaaatggct
     1501 taataattca ggggtaacag gcaaatcctt tataagtgtg gcaaagtcca ttcaacaagc
     1561 tgaaatgaag atgaaaccag gtcagaaaaa atggatgaag gcatcagccg ttcagtattc
     1621 ttttattgat gtacctgctt ttatctggat agctgatgtg aaagtgaatc cattcctgag
     1681 ttttgcagga agagacagat ttatagatgg caaaggggaa atgttgatta agttgaattc
     1741 tctgatcaat atagtatatg aacagggaga gaaacttgat gaaggagctt tacagcgtta
     1801 tctgggcgaa atgatatggt tcccttcact tgcactcagt ccttttatca cctggcagca
     1861 tattgatgac aatactgtta ttgcaactat gagtttcaac ggaacaacag gaagcggcac
     1921 tttttatttt gatagcaatg gagattttgt aaagttttcg gcattgagat acatgggtaa
     1981 caaaccggat tcaaaacgat acgaatgggt gatactggtt gacggatata agactttcga
     2041 aggcataaag gtaccttcaa aaatgaccgc tacatggaag cttgaagagg gggactggac
     2101 gtggcttaaa ctggagatta ttgagattaa gtataactag attgtaaatc ttataaatac
     2161 cggataattt tgttaataat agaataactt gcctgcacag agattttcgg ttgttatttc
     2221 cagtctaaat aacaaagaaa ataggcaaat gaaataaatc gaatttttct ttatgaaacg
     2281 acccgctttc atatttccat atccggctta cgacagtcag acatgcccca tggataatgc
     2341 caaatgacat taaattggag ttacgggtaa atcaaccttt catagggtcc ggaggttgcc
     2401 cgctccttcc catttccaaa ttctccatta tcttcatcgc ctcctccctg ctacttccgc
     2461 agaaatccgg atcgggccta aaatcaatac atacttttgg tcgaagttca cttccataca
     2521 gtgcgcagcg gtaatcgtca gtgagatgta tgcatcttat acccgcaggt ttgccacagg
     2581 gcatacctgg aatgggcgat gatattgaaa tcgcaataca gcaggctcca caataggggc
     2641 gacactccac ttacagaaag agattaattc caaatattac aaaaatactt aattcagtgg
     2701 atcttcattt agtcagagga atgaggagtg agagatgagg gtgagctgag cagtattatc
     2761 gaacagaaag tttatattaa ttaattaagt aaattataat attttaaact cataaacatt
     2821 ttatatcaga ctaaaacttc ccgaaatgaa aactcagttc ttgccggccg tttttactat
     2881 aatcctgatg gtctttttaa tagattattt atcaggtcaa tctgcaggtc agctggcaac
     2941 agggaaaaaa ttcattaacg cccgcgtgac agaggttgta agggttaaaa caggtatcgg
     3001 tcaggaaaat atgggtgccc tcagtccgcc cgaagctgag gcaaatgttc ctatgagctt
     3061 cacattcagc gattaaggac agaatcaatt aaactcgaga gttcaagtat aacgcgatgt
     3121 aaaaagaaca gttacaataa agactctgtt ttggtatttt tagggagtca aaatataact
     3181 ttcaagttgg taataggcga ttctttttac caaacatgat aaaaatgaag aagaaaaaat
     3241 acgttttgaa tcattgtaaa tttgtatttc agtgaatcat tatacctccg gtttttacta
     3301 ataatccaat aaaaaattca gttgatcaag caaaacttct taaatttgta ttgcaataaa
     3361 aaagtgctga tttcaaattc cataaaaata tacaaaatag tatagtttat tttgagttaa
     3421 gtgtatatca tttttttcta gattgatttt attaaatgaa aaagcgcctt ataattgttg
     3481 caaaccggct tcctgtaaaa gttacgcaga tcgatccttt tacattacag gaatctgaag
     3541 gaggtttggc cactggtcta aactctcttg atactaatta tgagaagcac tggataggat
     3601 ggcctggtgt gtatgccaag aacaggaaag agcgttcaat tatcttagaa gcactgaaga
     3661 agaaaaacat tcatccagta tttctgagcc ggcagcaggt ggaacttttc tatgaaggat
     3721 attgcaacgg cactctctgg cccttgttcc attattttta tgtctacact gtttacaatg
     3781 aaaaatatta cagcgtatat caggaggtta acagactttt tctcgaaaaa gtgctcgagg
     3841 tagccagaga aggtgatatt ttgtggatac atgattatca tctattgatt cttccgggta
     3901 tgatacggga aaaatggaaa aatgcttcaa ttggtttttt cctgcacatt ccattcccgt
     3961 catacgaact tttcaggttt ctgccagaac gaaaggagat cctccgcggg ctattagggg
     4021 ctgatctggt gggttttcac acctatgatt acatgagaca tttccagagt actgtttacc
     4081 gtatccttgg gtatgaattc aaactaaatg aaatgatcat tgatagccgt atggtgagga
     4141 taaactcttt tccgatggga ataaaatttg agaagttcta ccattcttcg gaaaatg
//
LOCUS       DAOSNB010000231         4187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21310_length_4187_cov_3.887948, whole genome shotgun sequence.
ACCESSION   DAOSNB010000231 DAOSNB010000000
VERSION     DAOSNB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21310_length_4187_cov_3.887948"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="PLG42_08945"
     CDS             complement(<1..126)
                     /locus_tag="PLG42_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOU31374.1"
                     /translation="MDFEKISATYFVGIGGIGMSALARYFLSKGCIVAGYDRTRSP"
     gene            complement(126..1238)
                     /gene="murG"
                     /locus_tag="PLG42_08950"
     CDS             complement(126..1238)
                     /gene="murG"
                     /locus_tag="PLG42_08950"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOU31375.1"
                     /translation="MNRQKNKRVIISGGGTGGHVFPAIAIANALKKLEPSLEILFVGA
                     LGRLEMELVPKAGYNIVGLPVAGINRKNPLKNISVLWKFIKSLRTAGTILRDFKPDVV
                     VGVGGYASGPVLKKALSMKIPVLIQEQNSYAGITNRLFAKKASVICVAYDGMEKYFPA
                     SRIIKTGNPLRQSLGDIEGLKDEALRYFNIKSDRPVILVLGGSLGAGSINKCLAENIE
                     KIRESGLYWIWQTGRNYFEDINALVSVTQSGNISVYPFIERMDLAYSAADIIISRAGA
                     GTISELCLVGKPVILVPSPNVAEDHQTRNAMALAEKEAAVIVRDSEVNQKLIDEVLFL
                     ADDIGKRNKLSQNIRKLAMADADLRIAEEVIKLMDR"
     gene            complement(1219..2439)
                     /locus_tag="PLG42_08955"
     CDS             complement(1219..2439)
                     /locus_tag="PLG42_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOU31376.1"
                     /translation="MSKGWVANTFKGDRVIWALVALFMFYSLLAVYSSSAIVAYSRFG
                     GNTTYFLKTQLIMLIFGLVIIIITHHLPVRIYSSLAGVFLIFSVALLVLTLIFGVRIN
                     EATRWIVIPGTGFRLQSSDVAKVALVIYLARTLAHYQHELENFGTVSKYLMLPVAIVC
                     SLILTENLSTAVMIFGISILILYIGRVPVRFLLAYVGIAIVTLLLAAFLISVIWPHNN
                     RVEVWKNRIENYFSSKDDNTDEDYQINQAKIAIATGGLFGKAPGKSTQRNMLPQSNSD
                     FIFAIIIEEYGLLFGAVPIILAYMILLFRGIAIAKKCETAFPAFLVMGLTLMIVVQAL
                     INMLVATGLLPVTGQTLPMISWGRTSVLVMSFSIGAILSVSRVVYARIRNEELTEEEK
                     FEEGDEKIYEPAKK"
     gene            complement(2446..3780)
                     /gene="murD"
                     /locus_tag="PLG42_08960"
     CDS             complement(2446..3780)
                     /gene="murD"
                     /locus_tag="PLG42_08960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOU31377.1"
                     /translation="MKRIVILGGGESGTGSAVLARKHGFDVFVSDKGRLKTEYRDILK
                     KYNIRFEEGAHSEDIIFTADEVIKSPGIHDDMPLVMELRKKGIPVISEIEFAGRFTDA
                     MKICITGSNGKTTTTTLIWHILKKAGINAALAGNVGNSFAMAVASGGYDCYVIELSSF
                     QLDGMFDFKADIAVLLNITPDHLDRYGHDMSRYVDSKFRIIQNQTENDHLIYWADDPV
                     IIRELEKRQPPVKLLPYSATKSEGMAGYIENDEIIIHLKHKTRLMTIHDLALKGRHNT
                     LNSLAAAIAGKIMNIRKEVIRESLADFQGIEHRLEPVITVCGINFINDSKATNVNSTW
                     YALECMQTRVVWIAGGIDKGNDYSDLFEVVSKKVKAIVCLGKDNRKIIEAFKDKVPTI
                     VETTSMEEAVRSAYYLASKGDTVLLSPACASFDLFENYEDRGRKFKAAVRNL"
     gene            complement(3786..>4187)
                     /locus_tag="PLG42_08965"
     CDS             complement(3786..>4187)
                     /locus_tag="PLG42_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOU31378.1"
                     /translation="PGSEELVIFASAFIGATVGFLWYNSYPAQVFMGDTGSLALGGII
                     AVFAIVIRKELLIPVLCGIFLAENLSVVIQVSWFKYTRKKYGEGRRIFLMSPLHHHYQ
                     KKGYPEPKIVTRFWIVAIILAVLSIVTLKIR"
BASE COUNT         1164 a          978 c          785 g         1260 t
ORIGIN      
        1 agggcttctg gttctgtcat agccagcaac aatgcagcct tttgacagaa aatatcttgc
       61 cagggcactc attcctattc ctcctatgcc cacaaagtac gttgcagaaa ttttctcaaa
      121 atcaatcatc tgtccatcag tttaataacc tcttctgcaa ttctcaggtc ggcatcagcc
      181 attgccagtt ttcggatatt ttgcgacagt ttgtttcttt tcccaatatc atctgcaagg
      241 aaaagaactt catctatcag tttctggtta acctcactgt ctctcacaat aactgctgct
      301 tctttttcgg ccagagccat agcatttctt gtctgatgat cttccgcaac atttggtgag
      361 ggtaccagaa ttaccggttt accgaccaga cagagttccg aaatagtccc cgcacctgca
      421 cgcgaaataa ttatgtctgc agcagaatat gcaaggtcca ttctttcaat aaaaggatat
      481 acagaaatat ttcctgattg tgtcacactc accagagcat taatgtcctc gaaataattc
      541 ctgcctgttt gccatatcca gtataaacct gactccctga ttttttcaat attttcggcc
      601 aggcatttat ttatgcttcc cgctccgagt gatcctccca gtaccagaat aacaggtctg
      661 tcacttttta tattgaaata tctcaaggct tcgtctttca atccctcaat atcaccgagc
      721 gactgcctta gaggatttcc tgtctttatt attctggaag ccggaaaata tttttccata
      781 ccgtcataag ccacacatat tacagatgcc ttttttgcaa agagccggtt ggtgatgcct
      841 gcataactgt tctgttcctg gattaaaacc ggtattttca tcgacagagc tttctttaga
      901 acaggtccgc ttgcatatcc gccaactcct accacaacat ctggtttaaa atctcttaga
      961 atagtgcctg ctgtcctcag gcttttaata aatttccata agacagaaat atttttaagc
     1021 ggattttttc tgtttattcc agcaacagga agtccgacaa tattataacc ggctttggga
     1081 accagttcca tttcgaggcg tccaagagcg ccgacaaaaa gaatttccaa cgatggttcg
     1141 agttttttca gggcatttgc aatagctatt gccgggaaga catgcccccc ggttcctcct
     1201 ccgctgataa tcaccctttt atttttttgc cggttcatat attttttcgt ctccttcttc
     1261 aaatttttcc tcttcagtca gttcctcatt ccttatccgc gcataaacca ccctgctcac
     1321 gctcaaaata gccccaatgg aaaaactcat gacaagtacc gaagtacgtc cccagcttat
     1381 cataggtagt gtctgtccgg tgacaggaag aagtccggta gctacaagca tattgataag
     1441 agcctgtaca acaatcatta atgtaagtcc catcacaaga aaagcaggaa aagctgtttc
     1501 gcacttttta gcgatagcta ttcctctgaa aagcaatatc atataagcca gaatgatggg
     1561 aactgcaccg aaaagaaggc catattcttc aataataatt gcaaaaatga aatcagaatt
     1621 cgattgcggt agcatgtttc tctgggtgct cttaccagga gcttttccga atagtccacc
     1681 cgttgctata gctatttttg cctggtttat ctggtaatcc tcatccgtat tatcatcttt
     1741 tgatgaaaaa tagttttcaa ttctattttt ccagacctca actctgttat tatgaggcca
     1801 tattactgaa atcagaaaag cagcaagcag cagagtgaca attgcgatac cgacgtaagc
     1861 aagcaggaat ctgacaggaa ctctacctat ataaagaata agtatactaa ttccaaaaat
     1921 cattactgct gttgagagat tctcggtgag aataaggcta catactattg caaccggcag
     1981 catgaggtat tttgagactg ttccgaagtt ttcaagctca tgctggtaat gtgccagtgt
     2041 tctggcaagg taaattacaa gtgcaacttt tgccacatca gacgattgca gcctgaagcc
     2101 ggtgccaggt attacaatcc agcgagtagc ttcatttatt ctaacaccga aaataagtgt
     2161 gagaaccaga agcgcaactg agaaaatcag aaaaacaccg gcaagagaag aatatattct
     2221 aaccggcaag tgatgggtga taataattat aaccaggccg aatatcagca ttataagctg
     2281 ggtcttaaga aaatatgtag tattacctcc aaacctggag taagcaacta ttgctgaaga
     2341 gctgtatacg gccagcaatg aataaaacat aaagagggct accagggccc agattaccct
     2401 gtcgccttta aaagtattgg caacccatcc cttactcatt gtcttttaca gatttctaac
     2461 agcggctttg aatttgcgcc ctctgtcctc ataattttca aacaggtcaa aacttgcaca
     2521 tgccggagaa agaagaacag tatctccctt tgaagcaaga taatatgccg accttaccgc
     2581 ctcttccata gaggtggttt caactatggt aggcaccttg tctttaaaag cttcaatgat
     2641 ttttctgtta tcctttccga ggcagacaat tgcctttacc ttcttactga ccacttcaaa
     2701 aaggtcggaa taatcattgc ctttgtctat tcctccggca atccagacca ctctggtctg
     2761 catacattca agggcatacc aggttgagtt aacgttggta gcctttgagt cgtttataaa
     2821 gtttataccg caaacagtaa ttaccggttc gagcctgtgc tcaataccct ggaagtcggc
     2881 aaggctctcc ctgatcactt ccttacgtat attcattatt ttgcctgcaa tggctgccgc
     2941 cagtgagttc aatgtattat gacggccttt taacgcaaga tcgtgaattg tcataagtct
     3001 ggttttatgt tttaggtgga taattatttc atcgttttcg atatatcctg ccattccttc
     3061 gctctttgtg gcagaataag gaaggagttt taccggaggc tggcgttttt caagttccct
     3121 gataataacc ggatcatcgg cccagtagat taaatggtcg ttctcagtct ggttctggat
     3181 tatccgaaac tttgaatcaa catacctgct catgtcatgc ccgtagcggt caagatgatc
     3241 aggtgtgata ttaagcagta ctgctatgtc ggctttgaaa tcgaacatac catcaagctg
     3301 gaaactgctg agttcaataa catagcaatc ataacctcct gaggcaactg ccattgcaaa
     3361 actgttccca acattcccgg caagtgctgc attaatacca gcctttttca gaatgtgcca
     3421 tataagggtt gtcgtggtgg tttttccgtt actgcctgta atgcatattt tcatcgcatc
     3481 agtgaatctg cccgcgaact caatttcaga aatgacgggt ataccttttt tcctgagttc
     3541 catcaccaga ggcatgtcgt cgtgtattcc cgggctttta ataacctcat cagcagtgaa
     3601 tattatatct tcactgtgag cgccttcctc aaacctgata ttatattttt taagtatgtc
     3661 tctgtactca gtttttagtc ttcctttgtc agaaacaaaa acatcaaatc cgtgttttcg
     3721 tgcaagcacg gccgaaccgg ttccgctttc tcctcctcca agtattacaa ttcttttcac
     3781 tgttgctatc ttatctttaa tgttactata ctcaatacag ccagtattat tgccacaatc
     3841 cagaatcgtg tgactatttt cggttcagga taacccttct tctggtaatg gtggtgcagc
     3901 ggcgacatta gaaaaattct ccggccctca ccgtatttct ttctggtata tttgaaccac
     3961 gacacctgta taactactga aagattttcg gcgaggaaaa taccgcagag caccggaata
     4021 agaagttcct ttctgataac tattgcaaac actgctataa tgcctcccag ggcaaggcta
     4081 cctgtatcgc ccataaacac ctgagcggga taagaattgt accaaagaaa tcctacagta
     4141 gcacctatga aagcacttgc gaagatcacc agttcctcag aaccggg
//
LOCUS       DAOSNB010000232         4163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21443_length_4163_cov_4.099075, whole genome shotgun sequence.
ACCESSION   DAOSNB010000232 DAOSNB010000000
VERSION     DAOSNB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21443_length_4163_cov_4.099075"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLG42_08970"
     CDS             <1..259
                     /locus_tag="PLG42_08970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOU31379.1"
                     /translation="ALIKLTKYIMLFILPAFLTALLLNGFVLKGIGILWAVVKILGIV
                     LLLTLIRNTNPRLKIKQAVSFFLVWMNLLAILAIVLAYFGY"
     gene            271..705
                     /gene="nuoB"
                     /locus_tag="PLG42_08975"
     CDS             271..705
                     /gene="nuoB"
                     /locus_tag="PLG42_08975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOU31380.1"
                     /translation="MGFKSFCFKKSLWVFHFGGASCNNCDIEILDCLTPRYDLERFGI
                     LLVGSIRHADVLLVNGSINMRDRDRLIEIYNQAPKPILVVAIGACGCTGGIFAEGNTF
                     AGPVDKIIPVDAYIPGCPPKPEAIIAGLVKLVNSSNPFKRDK"
     gene            751..1194
                     /locus_tag="PLG42_08980"
     CDS             751..1194
                     /locus_tag="PLG42_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOU31381.1"
                     /translation="MTTEEIITGLKEKFGPEITDTIRRNEKRYVVSINPGALLKVADY
                     LYREAGCRFIIATALHTKRGFEIHYHFSKDATGLVLNIHVVLSHEKPQIESLSNMFNA
                     SNWIEREMHELFGIDFLNHPNQEKLISEGNWAEGVYPYRKDFKKN"
     gene            1196..2392
                     /locus_tag="PLG42_08985"
     CDS             1196..2392
                     /locus_tag="PLG42_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU31382.1"
                     /translation="MSKKFLIPIGPYHPLQEEPELFKLYCEGEIVKDIKWETGYNHRG
                     IEKLSESKTYDQVFFLVERICGICSTSHPFAFANAVEEICRIEVPDRAKYIRSIIGEL
                     ERIHSHLLWVGLAGHFLGYNTVFMWAWKYREPVLDLFEMITGNRNSYAMFKVGGVRRD
                     IEAAKIPAIRKVLAEVKKKTDLLTGAVMDDPVIHARTKGVGILTKEDILDYGAVGPTA
                     RASGVAIDVRKDDPYAAYPLVNWKVITAEEGDVFAKAKVRLLEMYESISIIEQCLDGL
                     LKEKEGDIAVKVKNIPEGMGTGRAEAPRGEVFHCVFSDGSNSPARHKIRAPSYVNIAT
                     FKKSCIGQSISDATISLAAVDPCYCCTERMAVAYDISTGKRIMNGAEILKASAEKTRE
                     IMSKLK"
     gene            2397..>4163
                     /locus_tag="PLG42_08990"
     CDS             2397..>4163
                     /locus_tag="PLG42_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOU31383.1"
                     /translation="MNEYFSIIVLPVVAGILLFLVPDKIKTAKGIFSLLISAFTFWLA
                     FKIYNFDEAKLDLKALLPASLIPASLSPGISSFFAFRCDNLAKLIILFVSLFTFLILL
                     YSLVYVRGGRIKNYYEYFMLTLGCSYGAVLSDNLLIFLTFWGILGITLYKLIQGNNEE
                     SSATAKKTLILIGASDSIMIIGIAILWKMTGFINMSEIQVPTGTALSCTAFLALLIGS
                     FTKAGAFPFHTWVPDYAKDAPASSSAYLPASLDKLLGIYFLARIVTGLFIPGEWMTLL
                     LLTLGVATIITAVMMALIQHNFKQLLGYHAVSQVGYMILGLSLGSFIGIAAGLFHMVN
                     HALYKSGLFLSAGAVEHRTGKNDLEDVGGLSKAMPLTFFAALICAMSISGIPPLNGFA
                     SKWMIYQAIIDFGGGDGLPQKLWVLWLVLAVLGSALTLASFIKFIGGIFLGRRKPEFE
                     SVKEVPAPMWIPFTVIALLCIIFGVFATRLVVPHLLMPVTGEFEFSGFWNSGFVSLLV
                     LVSIVAGALIYLATGIKKFRTSDSFIGGEKFYEQTAYPAPEFYKTLLEFRFLSSIYRK
                     AQEKWFDIYDLSKKAVLWCSHVL"
BASE COUNT         1178 a          894 c          965 g         1126 t
ORIGIN      
        1 tgctctgata aaactgacga aatacataat gctgttcatt cttccggctt ttctgacagc
       61 cctgctactg aacggatttg tactaaaagg aataggtatc ttatgggctg tagtgaaaat
      121 ccttggaatt gtgctcctgc ttactcttat caggaacaca aatccacgac tgaaaattaa
      181 gcaggcagta agtttcttcc tcgtatggat gaacctgctt gctattctgg caatagttct
      241 tgcatatttt ggatattaaa aaacagatac gtgggattca aaagtttttg ttttaaaaag
      301 tcactctggg tgttccattt cggcggtgca tcgtgtaaca actgcgacat agagatactc
      361 gactgtctta caccgagata tgatctggaa cgtttcggca ttctgctcgt tggcagtatc
      421 agacatgccg atgtgctgct ggtcaacggt tcaattaata tgcgcgacag ggaccgtttg
      481 attgaaatct ataaccaggc ccccaaacca atccttgtgg tggcaatagg tgcatgcgga
      541 tgcacagggg gtatatttgc cgaaggtaat acgtttgccg gacctgtcga caaaataata
      601 cctgtcgacg cctatatacc cggctgccct ccgaaacctg aagcaattat tgcaggcctg
      661 gtgaaattgg taaacagtag caatccgttt aaacgtgata aataacggtt agaataaact
      721 gcatgaattg aataaccaga aacaacataa atgaccactg aagaaataat aacaggatta
      781 aaagaaaaat ttggcccgga gataacggac acaattagac gtaatgaaaa acggtatgtg
      841 gtatcgataa acccgggtgc cttgctgaag gtggctgatt atctctacag ggaggctggg
      901 tgcaggttta ttattgcaac tgcacttcat acaaaaagag gctttgaaat tcattaccat
      961 ttcagcaaag atgctaccgg attggtacta aacatccacg ttgttctgag ccacgaaaag
     1021 ccgcagattg aatcgttatc aaacatgttt aacgcatcca actggattga aagggagatg
     1081 catgaactgt tcgggatcga ttttctgaat catcccaatc aggaaaaact gatatcagaa
     1141 ggtaactggg ctgagggagt ttatccttac agaaaagatt ttaaaaagaa ctgaaatgag
     1201 caagaaattt ctcataccaa ttggtcctta tcaccccctt caggaagagc cagagctttt
     1261 caaactttac tgtgagggag agatagtaaa ggatataaaa tgggaaacag gttataacca
     1321 caggggtatt gaaaaactct cagaatcaaa aacatacgac caggtctttt tccttgttga
     1381 aagaatatgc ggcatctgtt ccacgtcgca tccttttgcc tttgcaaatg ctgtggagga
     1441 aatatgcagg atagaagtgc ccgaccgtgc aaagtatata aggagcatca tcggggaact
     1501 ggagcgcatt cacagtcacc tgttgtgggt ggggctggca ggccatttcc tcgggtacaa
     1561 cacggttttt atgtgggcat ggaaataccg cgaaccggta ctcgacctct ttgagatgat
     1621 tacaggtaac cgcaacagtt atgcaatgtt caaggttgga ggtgtaagaa gggacattga
     1681 ggctgcaaag atacctgcca ttcgcaaggt tctggcggag gtaaaaaaga agaccgacct
     1741 gctgaccggc gcggtaatgg atgatcctgt gatacatgcc agaacgaaag gtgtgggaat
     1801 actaacaaaa gaagacattc tcgattatgg tgcggttggt cctactgcaa gggcatcggg
     1861 cgtagcaata gatgtgagga aagatgaccc ttacgccgct tatccacttg tgaactggaa
     1921 agtcattact gccgaggaag gggatgtgtt tgcaaaggca aaagtgaggc tgctcgaaat
     1981 gtatgaatca atatcaatca tcgagcagtg cctcgacggc ctgcttaagg aaaaagaagg
     2041 tgatatagct gtgaaagtaa aaaacattcc tgaaggaatg ggcaccgggc gtgcagaagc
     2101 accacgcgga gaagtattcc actgcgtctt ctccgatggt tcaaattcac cggcacgcca
     2161 caaaataagg gcaccaagct atgtcaacat tgccaccttc aagaaatcat gcatcggtca
     2221 gagcatttcc gatgccacaa tatcgcttgc cgctgttgat ccatgctatt gctgtaccga
     2281 acgcatggcg gttgcgtacg acatcagcac tggtaaaaga attatgaacg gggctgagat
     2341 actgaaagcc tcagcagaaa aaacccgtga gattatgtca aaactaaaat gagagtatga
     2401 acgaatactt ttcaataata gttctcccgg tcgttgcagg aattcttctc ttcctggttc
     2461 ccgacaaaat aaaaacggca aagggaattt tctcactgct aatcagtgcc tttaccttct
     2521 ggctggcatt caaaatttac aactttgatg aggcaaaact ggacctcaaa gctttattgc
     2581 cggcttcact gattccagcc tcactatcac ccggcatcag ctcattcttt gccttcagat
     2641 gcgacaacct ggcaaaactt atcattctgt ttgtcagcct ctttacattt ctgatcctgc
     2701 tctattcgct ggtttatgtc agaggcggga ggattaaaaa ctattatgaa tatttcatgc
     2761 tcacgctggg ctgctcatat ggtgccgtac tttctgacaa tctgcttatc ttcttaactt
     2821 tctggggaat actgggtatt accctttaca aacttataca gggcaacaat gaggagagct
     2881 cggcaacagc aaagaagaca ttaatactga ttggagcgtc ggacagtata atgattattg
     2941 gtatagcgat tctctggaag atgactggct ttatcaacat gagtgaaata caggttccga
     3001 caggaacggc gctgagctgc actgcatttc tcgcccttct tatcggcagt tttacaaaag
     3061 ccggtgcctt tcccttccac acatgggtgc ccgactatgc taaagatgct cctgcatcat
     3121 catcagcata cctccctgcc tcactcgata aactccttgg aatatatttt ctcgcaagaa
     3181 tagtaaccgg actcttcata ccgggtgaat ggatgactct tctgctgctt acactaggag
     3241 ttgcaactat tatcacggca gtgatgatgg cactgattca acacaacttt aaacaactac
     3301 ttggctatca tgctgtttcg caggtaggat acatgattct tggattaagt ctgggctcat
     3361 ttatcgggat tgcagcagga ttgttccata tggtaaacca tgccctttat aaaagtggac
     3421 tcttcctctc agccggtgcc gttgagcatc gcaccggtaa aaacgatctg gaggacgtag
     3481 gagggctctc aaaggcaatg ccattgacgt tttttgctgc tcttatatgc gccatgtcaa
     3541 tctcgggtat tcccccactt aatggtttcg catccaaatg gatgatttac caggctatca
     3601 tcgactttgg cggcggagat ggccttccac agaaactctg ggtactatgg ctggttcttg
     3661 cagtactggg ttcggcactt acccttgcaa gtttcataaa attcattgga ggaatattcc
     3721 ttggaaggcg gaaacctgaa tttgagagtg taaaggaagt acctgccccg atgtggatac
     3781 ctttcacagt tatagcactt ctctgtatca ttttcggtgt ttttgccacc aggctcgtgg
     3841 ttcctcatct tcttatgccg gttacaggtg agtttgagtt ttcaggcttc tggaactccg
     3901 ggttcgtatc gctgctcgtt ctggtttcaa ttgttgcagg agcacttatt tatcttgcca
     3961 ccggcataaa gaaattcaga acatccgaca gctttatcgg aggcgaaaag ttttacgaac
     4021 agactgccta tcccgcacct gaattttaca aaacgctgct ggaattcaga ttcctttctt
     4081 caatttacag aaaggcacag gagaaatggt tcgacatata cgatctgtcg aagaaagctg
     4141 tgctgtggtg cagtcatgtg tta
//
LOCUS       DAOSNB010000233         4160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21466_length_4160_cov_4.286724, whole genome shotgun sequence.
ACCESSION   DAOSNB010000233 DAOSNB010000000
VERSION     DAOSNB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21466_length_4160_cov_4.286724"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..556
                     /locus_tag="PLG42_08995"
     CDS             65..556
                     /locus_tag="PLG42_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31384.1"
                     /translation="MSKKMKNIFKVVQLSRVLFFLVFIFGGITDAGAQFIKASDIFPS
                     EVKKQNAGRLNIYHEPQIDTLLSRYLLSNKLLGGGIEGYRIQIYRSAVRNAREEANKI
                     KADFMISFPDIPSYVEYEKPAYFIVRVGDFRTKMEGTKALYMIRKKYPNAYMIPCIIN
                     IPD"
     gene            571..2472
                     /locus_tag="PLG42_09000"
     CDS             571..2472
                     /locus_tag="PLG42_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOU31385.1"
                     /translation="MSEMIKHECGIALLRLRKKAEYYFDKYGTWTYGLNKMYLMMEKQ
                     HNRGQDGAGIAGIKLKSKPGVRYIFSEKSDKPDSITRIFQNIYENIYSVAPELQGKKY
                     NPEFIADEVPFLCDLYLGHLRYGTYGTYGLEHLHPVCRQNNWRSKNLLIAGNFNLTNV
                     PELFNHLVELGQTPPGFSDTVTILESIGYRLDEEHERLYNIYREQGYSKKEVARLIEE
                     NLDVAAILRKSGKNWDGGYVMAGLIGHGDAFVVRDPYGIRPAYYYVDDEVAVIASERP
                     VIQTVFNTDPESVKEVPPAHALIIKAGNEVRIEPVAEPVPPRKCSFERIYFSRGTDKS
                     IYCERKKLGELLTMPVLEAVGYDLENTVFSYIPNTAEAAFYGLLKGIEDYLNNLKVQQ
                     LLTERDELTEEKLREIISVRPRVEKITVKDIMLRTFITRDKSRDELVEHIYDITYGTV
                     RKGVDNLVVIDDSIVRGTTLRKSIIRILDRLGPKRIVIVSSSPQIRYPDCYGIDMAKL
                     GDFIAFRAAITLLKETGRASMIDDLYNRAKEEIKKPVNEVRNLVKEIYAPFTAEEISE
                     RISGLLKEPEINADITVVYQSVEGLHKACPDHMGDWYFTGDYPTPGGNRVAVQSYINF
                     IEGIDRRAY"
     gene            complement(2466..3428)
                     /locus_tag="PLG42_09005"
     CDS             complement(2466..3428)
                     /locus_tag="PLG42_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOU31386.1"
                     /translation="MKPVVDPVPREQIIAELSRIKLLRKTNNGNNEVYVFTYHDSPVL
                     MHEVGRIREITFRYAGGGTGKEIDIDEFDTDPENPQQQLIVWDPENKEIIGGYRFFFF
                     KEPCDKCDLSKLASSAYFNFSEKFKTKYLPHLMELGRSFVHPDYQSSNRGRKSLYALD
                     NLWDGLGAIIIDNPRIQYLFGKVTMYPHFDKKARSMILYFLKHHFPDKENLVTVKEPA
                     DIDLLEDEMKQLFTGKNYRENYKILSQEVRKLNETIPPLINAYMNLSPTMKTFGTFIN
                     HKFGNVEETGIMITLKDIYVEKINRHLASYNKDYEIKWFSRHNQ"
     gene            complement(3497..>4160)
                     /locus_tag="PLG42_09010"
     CDS             complement(3497..>4160)
                     /locus_tag="PLG42_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOU31387.1"
                     /translation="HAALNYLKVSYTVSGREHIPTRGRFIFVSNHPLGGLDGLVFIYE
                     LSKYFDNIKFPVNDILLNIKNFEDIFLPINKHGAQAREAARAIEEAYASDCQILYFPA
                     GLCSRKRKGVIKDLTWYKHFIKKAVTYKRDVVPAYFSGKNSNFFYNLANLRTFLGIKS
                     NIEMLYLADEMFRQKDKKIHLVFGPKIPWETFDKSKTPEEWAEWVKSKTYELEPLTRQ
                     EK"
BASE COUNT         1249 a          726 c          919 g         1266 t
ORIGIN      
        1 aatggttcat tttatattgt agcaagatcc tctgagattc tatctcccat ctaaaacttc
       61 gttgatgagc aaaaagatga aaaacatttt taaagtagtg cagcttagta gagtgttgtt
      121 tttcctggta tttatttttg gcggtatcac ggatgcaggt gcccagttca ttaaggcttc
      181 tgacatcttt ccttctgagg taaaaaagca gaatgcagga cgactgaata tttatcatga
      241 accacagatt gatacccttc tgagtcgcta tcttttatcc aataagcttc ttggtggcgg
      301 aatagagggg tatcgtattc agatttacag aagcgctgta agaaatgcaa gggaagaggc
      361 aaataagatt aaagcagatt tcatgataag ttttcctgat attccatcat atgttgaata
      421 tgaaaaacca gcttatttta tagtgagggt aggtgacttc cggacgaaaa tggaagggac
      481 aaaagccctc tatatgatca ggaaaaaata tcccaacgca tacatgattc catgcattat
      541 taacattccg gattagtaca ggatcagaat atgagtgaga tgatcaagca tgaatgcggt
      601 attgcgctac tgaggttacg caaaaaagca gaatattatt ttgataagta tggtacatgg
      661 acctacggcc ttaataaaat gtacctgatg atggaaaagc agcacaaccg gggacaggat
      721 ggtgccggaa ttgcaggaat aaaactgaaa tcaaagcccg gagtcaggta tatcttcagt
      781 gaaaagtcgg ataaacccga ttcaattacg agaatttttc agaatattta cgaaaatata
      841 tacagtgtag cacctgaact gcaggggaaa aaatataatc cagaatttat tgcagatgag
      901 gtgcctttcc tttgtgacct ttatctcggg catctcaggt atggcacata cggcacttac
      961 gggcttgaac atcttcatcc agtatgccgc cagaataact ggcgttcaaa aaatctgtta
     1021 atagcaggaa atttcaacct cacaaatgtt cctgaattat tcaatcatct tgtggaactt
     1081 gggcagactc cgccgggctt ttctgatacc gtcacaattc ttgaaagcat aggatacaga
     1141 ctcgatgagg aacatgaacg tctatataat atttacagag aacagggata ttcaaaaaag
     1201 gaagtagcaa ggctgattga ggaaaatctt gacgttgctg caattctcag aaagtcgggc
     1261 aaaaattggg atggcggtta tgtaatggca ggtttaattg gtcatggaga tgcgtttgta
     1321 gtaagagatc cttacggtat acgaccggca tattactatg ttgatgatga agttgcagta
     1381 atagcatcag aaaggcctgt tattcagaca gtatttaata ctgaccctga aagtgtaaaa
     1441 gaagttccac cagcgcatgc tcttattata aaggcaggaa acgaggtaag gattgaacca
     1501 gttgcagaac ctgttccgcc aagaaaatgc tctttcgaga gaatatattt ttcgagaggt
     1561 actgataaat ctatctattg tgaaagaaaa aaactcggtg aacttctgac aatgcctgtg
     1621 cttgaggctg tgggttatga tctggaaaac actgtctttt catatatacc gaacacagcc
     1681 gaagctgctt tctacggatt gctgaaagga attgaggatt accttaacaa tttaaaagtt
     1741 cagcaattat taactgaaag ggatgagctt acagaagaaa agctccgtga aattatttcg
     1801 gtaaggccaa gggtcgaaaa aatcactgtt aaggatataa tgctcaggac attcatcaca
     1861 agggataaaa gcagggatga gcttgttgaa catatttatg acatcacata tggaactgtc
     1921 agaaagggag ttgacaatct tgtggtcatt gatgattcaa tagtgagggg tacaactctg
     1981 agaaaaagta ttatacgtat tctcgacagg ttaggaccga agaggatagt tatagtctct
     2041 tcttcaccgc agataagata ccctgattgt tatggaattg atatggcaaa acttggcgat
     2101 tttatagctt tcagggcagc aattaccctt cttaaggaaa ctggcagggc ttcaatgata
     2161 gatgatcttt ataaccgggc taaagaagaa ataaaaaagc ctgttaatga ggttagaaac
     2221 cttgttaagg agatatatgc tccttttaca gcggaggaga tatctgaaag aatttcggga
     2281 ttgttgaagg aacctgagat caatgctgat atcacagtgg tatatcagtc ggtagaaggt
     2341 ctgcataaag cttgccccga tcatatgggt gactggtatt tcactggaga ttatcccaca
     2401 ccgggaggaa acagagttgc agttcagtcc tatataaatt tcattgaagg gatagacaga
     2461 agagcttact gattgtggcg tgaaaaccac tttatctcat agtctttatt gtaggatgca
     2521 aggtggcggt taattttttc cacgtaaata tctttcaggg ttatcatgat gcctgtctct
     2581 tcaacattgc cgaatttatg gttaatgaac gtcccgaatg ttttcattgt tggtgagagg
     2641 ttcatataag cattaataag cggaggaatt gtttcattga gctttcttac ttcctgagaa
     2701 aggattttat agttttccct gtaatttttt ccggtaaaca gttgtttcat ttcatcttca
     2761 agcaggtcaa tatctgcagg ctcttttacc gtaacaaggt tttctttgtc agggaaatga
     2821 tgcttcagga agtagagtat catacttcgt gccttcttgt caaagtgggg gtacatggta
     2881 actttaccaa aaaggtactg tatacgaggg ttatcaatga taattgctcc aaggccatcc
     2941 cacaaattat cgagagcata aaggcttttt ctgcctctgt tgcttgactg gtagtcgggg
     3001 tgcacgaagg aacgtccgag ttccattaaa tgtggaaggt attttgtttt gaatttttct
     3061 gagaaattga aatatgccga tgacgccagt tttgaaaggt cgcatttatc acacggttct
     3121 ttgaagaaga agaatctgta tccaccaata atttccttat tttcaggatc ccacactatc
     3181 agttgctgtt gtggattttc agggtctgtg tcgaattcat ctatgtcaat ttccttacct
     3241 gttcctcctc ctgcgtaacg aaatgttatt tcacgtatcc ttccaacctc atgcatgaga
     3301 acaggcgaat cgtggtaagt gaaaacatac acttcattat tcccgttatt tgtctttctc
     3361 agcagtttaa ttctggaaag ttcagcaata atctgctcac gtggaacggg atctacaacc
     3421 ggtttcattt ccttgtccat agtaagaaaa agaaaacaaa tttaaatttt ttgagaaatt
     3481 aacccaaggt ggtatcttat ttttcctgcc ttgtcagggg ctcaagttca taagtctttg
     3541 atttgaccca ttctgcccat tcttcaggtg ttttagattt gtcgaaggtt tcccatggta
     3601 ttttcggacc gaagactaaa tgtatcttct tgtctttttg cctgaacatt tcatcggcaa
     3661 gatagagcat ttcaatgttt gactttattc ctaaaaatgt tctcagattg gcaaggttat
     3721 agaagaaatt ggagtttttt cctgagaaat aagcaggtac aacatccctt ttataggtta
     3781 cagctttttt gatgaagtgc ttataccagg taaggtcttt tataacgcct tttcttttgc
     3841 gtgaacatag tccggctgga aaataaagta tctggcagtc ggaggcatat gcttcttcta
     3901 ttgctcttgc tgcttctctg gcctgtgccc catgcttgtt tatgggaaga aaaatgtctt
     3961 cgaagttttt gatgttgagg agaatatcgt ttacggggaa ttttatgttg tcgaaatatt
     4021 tcgacagttc ataaataaaa accagcccgt cgagacctcc gagtgggtgg ttagaaacga
     4081 aaataaatct gcctcgggtg gggatatgct cccggcccga aactgtatag cttactttga
     4141 ggtaattaag tgcagcatgg
//
LOCUS       DAOSNB010000234         4140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21630_length_4140_cov_4.743452, whole genome shotgun sequence.
ACCESSION   DAOSNB010000234 DAOSNB010000000
VERSION     DAOSNB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21630_length_4140_cov_4.743452"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /gene="atpC"
                     /locus_tag="PLG42_09015"
     CDS             <1..341
                     /gene="atpC"
                     /locus_tag="PLG42_09015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOU31388.1"
                     /translation="SSIYAGRDHRGCNRKRRKTSGTVKITNKMKIEIITPDDKIYEGD
                     IKSIRVPGSKGSFQVLKDHAPIVSTLDPGMIYIVDNAGNEIKYKISGGVIEVKMNKII
                     LLAESVLETS"
     gene            343..1761
                     /locus_tag="PLG42_09020"
     CDS             343..1761
                     /locus_tag="PLG42_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOU31389.1"
                     /translation="MNSSEFRKFAHELVDWMASYLENVESYPVKSNVKPGEIFSKLPD
                     TPPEKPESFENFFKDFINIIMPGITHWQSPNFFAYFPANTSPPSILAEMIISTLGVQC
                     MIWETSPAATELEQKMMNWLRDMIGLPENFEGVIQDTASTSTLAAILTAREKTTEFRS
                     NDEGLFNSEKLRIYCSEQAHSSVDKAVKIAGLGIKNLVKIGTRDDYSLDPEKLRIQIK
                     EDLKNKLIPCCVVATLGTTGVTAIDPLKEIGEICSEYRLWLHVDAALAGTALILPEYR
                     WMIDGKEYIDSFVFNPHKWMFTNFDCSAFFVKDASTLIRTFEILPEYLKTRTRGKVND
                     YRDWGVPLGRRFRALKLWCVIRMYGVEGLREMVRNHISIARRLAEMIKNEPDFELLAP
                     VTLNTVCFRYKPSGANENELNVLNEKLNHTLNDSGKMYLTHTKINGKYTLRMVTAQTN
                     VTMKHVMNSWEFILETARSMKA"
     gene            complement(1774..>4140)
                     /locus_tag="PLG42_09025"
     CDS             complement(1774..>4140)
                     /locus_tag="PLG42_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31390.1"
                     /translation="TILSESGRGRTPSFTSPLKAIAVEKGKMYSYDKMLLGSYRNYVP
                     DQYRYGYQVASWANEKYGHQLLRKSIEFTGRYPLSIFPVNISMSQSALLTKGKLHRET
                     FTRMAEKWRNEINDLQNSGTGIKILNSQRGKEYINYHSPVQIGTDSIVAFKTTMYKPP
                     AIVLINTSNGKEKRIHTPGYVNPYFLSGASGRVVWVEIHQGLRWENQQFSVVKILDVR
                     QGKTRQLTHRTRYMAAGISPDGRFVAAVENSISNSNSVVILDAFNGEIISRIQVPAGI
                     YPQRPQWSASGKEITFISLSDKGEGIILLDVEKQEWKLLKSPARDDLQSSFLKNDSLF
                     FVSSASGIDNIWILTSDGREVRVTDSRYGMYDLNISGENIICTEYSVNGKNICQLELG
                     RAGKIKIFDEKKYTGKSNLLSVSTEEGGTDNSKYIPVPYRKWLHPFRFHSWMPFYADI
                     DALKSDPSDLRPGFTLLSQNNLSTVISSLGYEYSNGNHILHSRLTLKGWLPVFESSVD
                     YGGAPDIIKSSDNIGDPSVINPALKVNNSLSLPLTFESGNYTSFLWTSFSLNYNNRYI
                     YLKEKGIYDYGQSEFTGRIYFSNYSHSGMRDIFPRWAQVADYSYTFFPADNEIYGPMS
                     TLRTALYFPGLLRNHGLRLRYESDFQEPVKFVLYNRASWPRGYDNIISQDLKFFSADY
                     VFPLLYPDLNIPGVVYIKRFRAGFFYDYAIGTNNTYIKESGNTFTKGKEYFRSFGTEF
                     ISDFYILRIPFMVSGGVQASWKDINEKPSLKIILNLDIYGMMIGRYKL"
BASE COUNT         1336 a          861 c          754 g         1189 t
ORIGIN      
        1 gaagcagcat ttatgctggt agggaccatc gaggatgcaa tcgaaaaagg agaaaaactt
       61 ctggcacagt caagataacc aacaaaatga agatcgaaat tataaccccg gatgataaaa
      121 tctacgaggg agatataaag tctataagag tgccaggcag caagggctca ttccaggtac
      181 tcaaagatca cgcaccaatt gtttctaccc tcgatcctgg tatgatatat attgtcgaca
      241 acgcaggtaa tgaaatcaaa tacaaaatat ccggaggcgt tattgaagtc aaaatgaata
      301 aaataatcct tcttgccgag tcagtactcg aaacatctta aaatgaattc aagcgaattc
      361 aggaagtttg cccatgaact agtggactgg atggcatcat accttgaaaa tgttgaaagc
      421 tatcctgtaa agtcaaatgt aaagcccggc gaaatattca gcaaacttcc cgatacacct
      481 cccgaaaaac cagagtcttt tgaaaatttc ttcaaagatt tcatcaatat tattatgcca
      541 ggcattaccc actggcagag ccctaacttt tttgcttact tccctgcaaa taccagcccc
      601 ccatcgatac ttgcagagat gattatttcc acacttggag tccagtgcat gatatgggaa
      661 acatcccctg ctgctacaga actggaacag aaaatgatga actggcttcg cgatatgata
      721 gggctacctg aaaattttga aggtgtgatt caggatactg cctcaacatc aacacttgct
      781 gcaattctga ccgccagaga aaaaacaacc gaattcaggt caaacgacga aggactcttt
      841 aattcagaaa aactaaggat atactgttcc gaacaggcac attcttcagt tgacaaagcc
      901 gtaaaaatag ccggccttgg aattaaaaat ctcgtaaaaa tcggaacaag agatgattac
      961 tctctcgatc ctgaaaaatt aagaattcag attaaagaag accttaaaaa caaattgatt
     1021 ccatgttgcg ttgttgccac tcttggcaca acaggtgtta cagcaattga tcctctcaaa
     1081 gaaataggag aaatatgctc tgagtataga ctgtggctgc atgttgatgc tgctcttgca
     1141 ggaacagcat tgattctgcc tgaatacaga tggatgattg atggcaaaga atacatcgac
     1201 agctttgtct tcaatcctca taagtggatg tttaccaact tcgactgcag tgcttttttt
     1261 gtcaaagatg cctcaacact aattcgtact tttgaaatac tccccgaata cctgaaaacc
     1321 cgaaccaggg gaaaagtaaa cgactaccgc gactggggcg taccgctggg aagaagattc
     1381 agagccctga aattatggtg tgttattaga atgtacggtg tggaagggct tcgggaaatg
     1441 gtcagaaatc atatctcaat tgcaaggaga ctggctgaga tgattaaaaa cgaacccgat
     1501 tttgaactcc ttgcacctgt cactctaaat actgtgtgct tcagatataa accctctgga
     1561 gcgaatgaaa atgagctgaa tgtattaaat gaaaaactta accatactct caacgattca
     1621 gggaaaatgt atcttaccca tacaaaaata aacgggaaat acacactgag aatggtgaca
     1681 gcccagacta acgtaacaat gaaacatgta atgaactcct gggaatttat ccttgaaacg
     1741 gcaaggtcaa tgaaagctta aacattacag tcattataac ttatatctcc caatcatcat
     1801 tccatatata tccaggttaa gtattatttt aagcgaaggt ttctcattaa tatctttcca
     1861 tgacgcctgg acaccccctg aaaccataaa aggtatcctt aagatgtaga aatcagaaat
     1921 aaattcggtc ccgaatgatc tgaaatactc ttttcctttt gtaaaagtgt ttcctgattc
     1981 ttttatgtat gtattatttg ttccaatagc ataatcatag aaaaaaccag ccctgaacct
     2041 cttgatgtaa accacaccag gaatattaag atcagggtat aaaagcggaa atacataatc
     2101 tgccgaaaag aatttcaagt cctgcgaaat aatattatca taacctcttg gccatgatgc
     2161 tctgttataa agtacaaact taacaggttc ctgaaaatcc gattcatatc tgagccttaa
     2221 accatgattc cgcaataacc ccggaaaata taaagcagta cgtaaggttg acattggacc
     2281 gtaaatttca ttgtcggcag gaaaaaaagt ataagaataa tctgccacct gtgcccagcg
     2341 aggaaaaata tcccgcattc ctgaatgaga gtaatttgag aaataaattc tgccggtaaa
     2401 ctcactctga ccgtaatcgt aaatgccttt ttctttcaag tatatatacc tgttgttata
     2461 attcaacgaa aaagaagtcc agagaaaact tgtgtaattg cctgattcaa atgtaagtgg
     2521 gagagaaaga gagttattta ctttcagggc aggatttata accgatggat ctcctatatt
     2581 atcagatgat ttaataatat cgggagcacc tccataatca accgacgatt caaaaacagg
     2641 aagccagccc ttcaaagtta acctggaatg taatatatga ttcccgtttg aatactcata
     2701 acccagagat gaaatcactg tactaagatt attttgtgaa agcagagtga atccaggtct
     2761 taaatcggaa ggatcactct tgagtgcatc aatatcagca taaaatggca tccagctgtg
     2821 aaacctgaaa ggatgcagcc attttctgta aggcactggt atatatttac tattatcagt
     2881 gcctccttct tccgttgaaa ctgaaagtaa attacttttt cccgtgtact ttttctcatc
     2941 aaaaatttta atttttcctg ccctgcctaa ttcaagctga caaatatttt tgccgttaac
     3001 cgaatattct gtacagatta tattttcccc cgatatatta aggtcataca taccatatct
     3061 ggaatcagtt actcgcactt ccctgccatc ggatgtcaaa atccagatat tatcgatacc
     3121 tgatgcagag cttacaaaga acagggaatc attcttaaga aacgaagact gtaggtcatc
     3181 tctcgcaggg ctcttaagca gtttccactc ttgcttttca acatctagta aaataattcc
     3241 ctccccttta tcagagagag aaatgaaagt tatttctttt cctgaagctg accattgcgg
     3301 acgttgagga taaatgccag ctggaacctg aattctgctt attatctcac cattaaatgc
     3361 atctaaaatg acaacactat tcgaatttga aatgctgttt tctactgcag caacaaatct
     3421 tccgtcgggt gaaatgcctg cagccatata ccttgttctg tgagtcaact gtctggtttt
     3481 tccctgcctg acatccagga tctttactac cgaaaattgc tggttctccc accttagccc
     3541 ctggtgaatc tccacccata caactctacc tgatgcgcca gatagaaaat aaggattcac
     3601 atacccgggt gtatgaattc tcttttcttt accgttagat gtgttaatca gtacaattgc
     3661 aggtggctta tacatggttg ttttgaatgc cacaatacta tcagtaccaa tctgaacggg
     3721 ggaatgataa tttatgtatt ctttcccccg ttgtgaattt aatattttaa taccagtacc
     3781 tgaattctgt aaatcattta tttcatttct ccatttctca gccattctgg taaatgtctc
     3841 tctgtgcagt ttgccctttg ttagtaaggc actttgagac atactaatat ttacaggaaa
     3901 tatagataat ggatatctcc cggtaaattc aattgatttt ctcagcagct gatgaccata
     3961 tttttcattc gcccatgatg caacctgata tccatatctg tattggtcag gaacatagtt
     4021 tctatatgaa cccagaagca ttttatcata actgtacatt ttaccctttt ctaccgctat
     4081 agccttcagg ggactggtga aagatggtgt tcgccccctc cctgattctg acaatatggt
//
LOCUS       DAOSNB010000235         4124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21726_length_4124_cov_3.584665, whole genome shotgun sequence.
ACCESSION   DAOSNB010000235 DAOSNB010000000
VERSION     DAOSNB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21726_length_4124_cov_3.584665"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..2416
                     /locus_tag="PLG42_09030"
     CDS             281..2416
                     /locus_tag="PLG42_09030"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HOU31391.1"
                     /translation="MKTLKKTTNRAISNKKSACISKKIRRCMMSSVLAILMLLWHHNS
                     FAQVPQGFNYQAIARDAGGNPIANTAMPVKITIQSEQTGGTVFWEELHSSVSTNNFGM
                     LTVVVGQGVKQSGTAATFADIDWSVTPKYIKTEIYYGGWKEMGVSPLWSVPYAHMSKS
                     SEQWQSSGSNLYRTTGNVGIGTNSPAGKLSIQPPSNWPDEQPLFEVKNKLGTPVLAVY
                     NNGVKILVEDTDGKGTKGGFAIGGFDPTKAGEETVNLMIVTPDSIRLNIDNTPTKATK
                     GGFAIGGFDASKSLSPREFMYVTPKASANGEYNTFIGYQAGFSNTSGTNNVFLGYQSG
                     YRNTTGSSNAFIGTLSGYNVTTGKRNTYMGHKAGYSNLSGSNGYSNVFIGDSSGYSNY
                     TGYRNVFIGSGVGPSNTSGYNNVFIGYEAGYANWGGYSNTFIGTEAGRNATLGYDNVY
                     IGYRTGYAGHGQYNVMIGHEAGLNNNGGSLNTFIGKSAGRENTDGDYNTLIGLEAGKN
                     ISTGNYNVAIGSFAGYNIQGGSGNIFIGNNAGYLETNPSNQLHIGSFPLIYGEFNSMV
                     VIMGKSNPNSNTFYVNGSAGGTSGWISASDMRMKTNIMPITDPLKKVCALRGVEFEWI
                     DKEKYDSKKHIGFIGQEVYDVLPEVVDNTNDFYSVQYGVITSLLVEAVKEQQKIIEAS
                     KLENQKLKSELEELRQKVASIEALLTEIK"
     gene            2461..>4124
                     /locus_tag="PLG42_09035"
     CDS             2461..>4124
                     /locus_tag="PLG42_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31392.1"
                     /translation="MIYRFITIFFVIFSALTQGQTIDSLSYSINPRYSFFSPGEEAEL
                     LLHIPQNHIYSAISVNVNFRGDTICEWNGKPGKNIIRLKFVTDTSWKSGKAEAKIHVQ
                     GQMQKMYYCSCDIIFLKHKPNEVKIDRLTGGLIVNGLPFFPFGFYTYSPVYPTLPEEE
                     VVKGFNLISPYQRLLPGSLGERKKYMDRCTQLGMKVNYNLASVAGGGGVDSGTNGMSD
                     EEKRQVLINEVKTFMDHPALLSWYIADEPDGHRIPPAYLEEIYKTVKETDPWHPVTIV
                     FMTPFTKAKDYAGATDIVMADPYPVPVYPVTMTGNATRMLKKEFEGRKPVWIVPQAFG
                     GGEHWLREPTIQEIRSMTWQAIAEGATGIQYFIRQGLNAFPKSTATWNECGRMAMEVN
                     AIAPWLLSDEVSIPATSSSSDISVASRMYKGLLIVIAVNKTSSPRPVQISINININTH
                     ARVLFENRTVQVTSGIINDYIQSYGSQVYKININPQKERIIPFRNNLIVDPGFEDLSS
                     PGIPSACYARPGSDRGATYFTDPRVVFEGNYSLRLVTPTENGSVTL"
BASE COUNT         1375 a          796 c          877 g         1076 t
ORIGIN      
        1 aagcggcaat ggcaatcgac aggtatttgc gcaaagaatt ctaattcagc acacttattt
       61 taatttcgtt gaacaggtaa aaaactgtaa ttctacataa ccccccttag tgtctttact
      121 tcaatattac aataggtgaa gtattggtaa aattttattt ttattgtaag tttgatataa
      181 taaagtaact tcatccatta agatacttta ttttcaaatg gtttttatac aaacctttat
      241 catattattt tgaatatcaa tatattaaaa atattatgat atgaaaactt taaagaaaac
      301 aacaaatcgt gcaatttcaa ataagaaaag tgcatgtatc agtaaaaaaa tccggagatg
      361 tatgatgtca tctgtgttgg caatattgat gttattatgg catcataact cttttgctca
      421 agtaccacag ggatttaact accaggctat tgcaagggat gcaggaggta atccgattgc
      481 taatactgca atgccagtga aaatcaccat tcagagcgaa caaacaggtg gaacagtttt
      541 ctgggaggaa cttcattctt cagtatctac aaacaatttt ggcatgttaa ccgtggttgt
      601 agggcaggga gttaaacagt caggtacagc agcaacattt gctgatattg actggagtgt
      661 aacacccaaa tacataaaaa ctgaaattta ttatggtggc tggaaagaga tgggtgtaag
      721 tccattatgg tcggtgccct atgctcatat gtcaaaaagt tctgaacagt ggcaaagtag
      781 cggttcaaac ctttacagaa caactggcaa tgtggggata ggtaccaatt caccagcagg
      841 gaaattatca attcaaccac cttcaaactg gccagatgaa caaccattgt ttgaggttaa
      901 aaataaattg ggtaccccgg ttcttgctgt atacaacaac ggagtaaaaa ttctggttga
      961 agacacagac ggaaagggga caaaaggagg atttgccata ggaggatttg atcctacaaa
     1021 agccggagag gaaacagtaa atctgatgat cgtgactccc gacagtatca ggcttaatat
     1081 tgataacacc ccaaccaaag caacaaaggg tggcttcgca attggcggtt tcgatgcttc
     1141 aaaatcatta agtccaagag aatttatgta tgtaacaccc aaagcatcag ccaacggtga
     1201 atataatact tttattggct atcaggctgg cttcagcaat acttctggaa ccaataatgt
     1261 tttcctcggc taccagtctg gatatagaaa tacaacaggt tcaagtaatg catttattgg
     1321 aaccctgtcg ggttataacg taacgaccgg caaaagaaat acatacatgg gtcataaagc
     1381 aggatattca aatttaagtg gatcaaatgg ttacagcaat gtgtttattg gcgacagttc
     1441 aggttattca aactatacgg gatatagaaa tgtcttcatt ggttcaggtg tcggaccaag
     1501 caacacaagc gggtataata atgtattcat cggttacgaa gccggctatg caaactgggg
     1561 aggatattca aatacattta tcggaactga agcaggccgt aatgctacac tcggttacga
     1621 caatgtatat atcggttaca gaacaggata tgcaggccat ggacaatata acgtaatgat
     1681 aggacatgag gcaggcttga acaataacgg cggaagtctg aacaccttta taggaaaatc
     1741 tgcaggaaga gaaaacaccg acggagatta caatactctt ataggattgg aagcaggcaa
     1801 aaatatttct acaggcaatt ataatgttgc tattggaagt tttgcaggat acaacattca
     1861 gggaggaagc ggaaatatat ttattggcaa taacgctggt tatttagaaa caaatccaag
     1921 caatcaactg catataggct catttccttt gatctacgga gagtttaatt cgatggttgt
     1981 aataatgggg aaaagtaatc ctaactcaaa tactttctac gtaaacggat cagcaggcgg
     2041 aacttcaggc tggatttcag cgagcgatat gagaatgaaa actaacatca tgccgattac
     2101 cgatccacta aaaaaagtat gcgcactgcg gggtgtcgaa tttgaatgga tagacaagga
     2161 gaaatatgac agcaagaaac atatcggatt tatcggacag gaggtatatg atgttttacc
     2221 ggaagttgtt gataacacaa atgacttcta ttcggtgcag tacggagtga taacctcact
     2281 gcttgttgaa gcagtaaaag aacaacagaa aataatcgaa gcctcaaagc tggaaaatca
     2341 gaagcttaaa tcagaacttg aggaactcag acagaaagtt gcttcaattg aagctttgct
     2401 gacggaaata aagtagatat ctcaggaaag ttattatttt taaaccggtg aactgttatt
     2461 atgatttacc ggtttataac tatatttttc gttatattca gtgcccttac gcagggacaa
     2521 acaattgatt cactaagtta cagtatcaat ccccgataca gttttttttc accgggagaa
     2581 gaagctgaac tgcttctcca tataccacag aaccatattt actctgcaat aagcgtaaat
     2641 gtcaacttca ggggcgacac tatatgtgaa tggaatggaa aaccaggaaa aaacataata
     2701 aggcttaagt tcgtcactga tacatcatgg aaatcaggaa aagctgaggc aaaaatccat
     2761 gtccagggac agatgcaaaa aatgtattat tgttcctgtg acattatttt cctcaaacac
     2821 aaaccaaacg aagtaaaaat tgacaggcta acaggcggtc tgattgtaaa cggtttacct
     2881 ttctttccgt tcggatttta cacctattcg cctgtttacc ccaccctgcc tgaggaagag
     2941 gttgtaaaag gcttcaacct tatttctcca tatcagcgac ttctacctgg atcccttggc
     3001 gaaaggaaaa aatatatgga ccgctgtaca cagcttggaa tgaaagtaaa ttataacctt
     3061 gcatcggtag ccggaggagg cggggttgat tcaggaacaa acggaatgag tgatgaagaa
     3121 aaacgtcagg tcctgataaa tgaagtgaag acctttatgg atcacccagc gcttctttca
     3181 tggtatattg ctgatgaacc cgacggacac agaatccctc ctgcctatct tgaagaaata
     3241 tacaaaactg tcaaagagac agacccatgg catccggtta caattgtctt tatgaccccg
     3301 tttacaaaag caaaagacta tgccggagca acagatatag tgatggccga cccgtatcct
     3361 gtgcctgttt acccggttac gatgacaggc aatgccacac gcatgctgaa gaaagaattc
     3421 gaaggaagaa agcctgtttg gatagtgcct caggcattcg gcggaggaga acactggtta
     3481 cgcgagccaa ccatacagga aatcaggtcg atgacgtggc aggccatagc tgaaggtgca
     3541 acaggaatcc agtactttat ccgacaggga ctgaacgcct tcccgaaatc aactgccaca
     3601 tggaacgagt gcggtcgaat ggcaatggag gttaatgcaa tcgcaccctg gctcctttcg
     3661 gatgaagtaa gcattcccgc aacttcatcc tcttcagaca taagtgtagc atcacgaatg
     3721 tacaaaggcc tgctgattgt aattgccgta aacaaaacct cctccccccg ccctgttcag
     3781 atatcaataa acataaatat caatacacat gcaagggttc tctttgaaaa ccgtaccgtt
     3841 caggtaacct caggtataat aaatgattac attcaatcgt atggttcaca ggtatataaa
     3901 atcaatatca atcctcaaaa agagagaata attcctttca gaaacaacct catcgtcgac
     3961 cctggatttg aagacctttc aagtccgggc attccttcag catgctatgc ccggccgggg
     4021 tcagaccgag gtgccacata ttttactgat ccgcgtgtcg tctttgaggg aaactattca
     4081 ctgagactgg ttactccaac cgaaaacggg agtgtaacac tcag
//
LOCUS       DAOSNB010000236         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_21958_length_4092_cov_6.604162, whole genome shotgun sequence.
ACCESSION   DAOSNB010000236 DAOSNB010000000
VERSION     DAOSNB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21958_length_4092_cov_6.604162"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1080
                     /locus_tag="PLG42_09040"
     CDS             400..1080
                     /locus_tag="PLG42_09040"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31393.1"
                     /translation="MKNPKFLIAVVVLLFEINLLNLQAQEQEVQNQKFNVNVDLYSNY
                     IWRGSKLGTGPAVQPSVKFSSGGLTIGVWGSFDADGYTEADPYFSYSFPFGLSLGMTD
                     YYLPDLPLFETSAASGSHALEMNGGFTKGGFSLSANIILNEAGGIASAGGDKYFQIGY
                     AFKYVNIFAGAGDGWHTSDGEFNLCNIGIGSSKVIRITDSFSVPLTGQIIFNPDKEKL
                     FLVAGFSF"
     gene            1115..1468
                     /locus_tag="PLG42_09045"
     CDS             1115..1468
                     /locus_tag="PLG42_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOU31394.1"
                     /translation="MKKVEVVIRKSKFDEVKDAMRKVGIEFITFWDVRGVGKATEERV
                     YRGIVYDTSTIERIMIIFFCREKYLEPAVNAILESAKTGEIGDGKIFISDIVDAYRIR
                     TGERGDKAMYLEGEE"
     gene            1489..2763
                     /locus_tag="PLG42_09050"
     CDS             1489..2763
                     /locus_tag="PLG42_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOU31395.1"
                     /translation="MIHTILQTDILSSLGGELENYAFSINTIWVLITASLVFFMQAGF
                     ALVEAGFTRSKNSTNILFKNLMDFVIGTIIFWAIGFGIMFGSKNGFWGGIDLFTRNTY
                     RTDMPDMAFLIFQTVFAATTATIVSGAMAERTKFNAYLIYSAIITLVIYPVSGHWAWG
                     GGWLSRMSVPFHDFAGSSVVHMVGGMAAMMGALTLGPRIGKYDKNGKAKAIPGHSISL
                     ATLGVFILWIGWFGFNPGSQLAAAGKVNASAISLIFVTTNISAAGGAVATMLYVWLKN
                     GKPTLSMTLNGALAGLVAVTAGCDSVSPGGALIIGLMAGVIVVLSVEFFDKVIKIDDP
                     VGAISVHGICGAYGTLMVGFFSTSQGLLYGNGGALLKSQLIGVASIASWAIFTSFVLF
                     RLLKLLNGLRVGKRIEEEGLDVYEHGETAYNN"
     gene            complement(2853..>4092)
                     /locus_tag="PLG42_09055"
     CDS             complement(2853..>4092)
                     /locus_tag="PLG42_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOU31396.1"
                     /translation="KSSIEEHIGEINYFRVYSGKVTENLDMINSNNSTKERLSQLYVC
                     AGKNRTRVPELNVGDIGAVVKMKNTKMDHTLNVPGNDWRYEGVIFPEPKFRTAIKAQS
                     ESDDEKLGEALNKIHLEDPTIKVEYSKELKQIIVHGQGEYHLNIMKWHLDNIYRIPTN
                     FNPPRIPYRETITKAAQADYRHKKQSGGAGQFGEVHMIIEPYVEGNPDLTMLKIGGKE
                     LKISVRGKDEIDLEWGGKLVYLNCIVGGSIDARFMPAILKGIMEKMEVGPLTGSYARD
                     IKVYVYDGKMHPVDSNEISFRLAGRNAFSQAFKNAAPKILEPVYDVEIMVPSDRMGDV
                     ISDLQGRRGMVLGMASEKRFEVIKARVPLAEMNKYSTALSSLTGGRAMYTMKFSEYAQ
                     VPGEVQEAVIKAYHEEEEEE"
BASE COUNT         1159 a          768 c          901 g         1264 t
ORIGIN      
        1 acgagtgagg gatctcctcc tttcggcaga gaataaaaga aagatgtgta taaagagtag
       61 ctcaaagtat agtggatgat tagaaataag gaaagccttt caataacctg gtattgaagc
      121 aaagccgtct caaaatgtta tttagatggt attttttccg gttatcaaca attttaaact
      181 tttagaatgc gcgtgggctt ctttttatgt caaccctaaa aaaatagggg gtataataga
      241 aaaaaacata aaaaagtatc tataaccccc ctaaaaaatg gggtataaat gaaaaaaaga
      301 ataaaaaaat taaatatacc cccataaaaa gaggtggtat attaaaatat ttatattttt
      361 gcatcgatta ttttattatt aacacttaaa aaattagtta tgaaaaatcc gaagttttta
      421 atcgcagtag tggtgttgtt gtttgaaatt aaccttttaa atctccaggc tcaggaacag
      481 gaagtacaga atcagaaatt taatgtaaat gtcgatctgt actcaaatta tatatggagg
      541 ggttcaaaac ttggaacagg tcctgcagtt caaccttcgg tgaaattctc gagcggggga
      601 ttaacaattg gtgtgtgggg ctcctttgat gcagacggat acactgaagc agatccatac
      661 ttttcatact cctttccttt tggccttagt cttgggatga ctgattatta tctccccgat
      721 ctgcctttgt tcgagacttc tgcagcttca ggaagtcatg ccctggaaat gaatggaggt
      781 tttacaaaag gaggcttttc attaagcgca aatattattt tgaatgaagc cggaggcata
      841 gcatcagccg ggggcgataa gtattttcag atcgggtacg cctttaaata tgttaacatt
      901 tttgccggag ctggtgacgg atggcacaca agcgacgggg aattcaattt gtgcaacatt
      961 ggtattggtt cttcaaaagt gatacggatc acagatagtt tttcagttcc gctgaccggg
     1021 cagatcattt ttaatccgga taaagagaaa ctctttttgg ttgccggttt ctcattctga
     1081 aaaaggtcaa atttaaaaaa tacagaaaat gaaaatgaag aaggttgaag tcgtaataag
     1141 aaaatcgaag ttcgacgaag tcaaagatgc catgcgtaag gtcggaattg aatttataac
     1201 attctgggac gtaagaggcg ttggtaaagc tacagaagaa cgtgtttata gagggattgt
     1261 atatgataca agtacaattg aaagaataat gataattttt ttctgtcggg aaaagtatct
     1321 tgagcctgct gtaaatgcaa ttcttgaatc cgcaaaaaca ggagagatcg gagatggaaa
     1381 gatattcatc tcagatattg ttgatgctta caggataaga acaggggaaa gaggagataa
     1441 ggcaatgtac cttgaaggag aagaatagta attaaaaatc taactaaaat gatacacaca
     1501 atattacaaa cagatatttt aagttcactt ggtggagaac ttgaaaatta cgcgttttca
     1561 attaatacta tctgggttct tataactgca tctctcgtat tctttatgca ggccggattt
     1621 gcacttgttg aagcaggttt tacccgttct aaaaattcta caaacatcct tttcaaaaac
     1681 ctgatggatt ttgttatcgg gaccataata ttctgggcaa taggtttcgg gataatgttt
     1741 gggagcaaaa atggtttctg gggagggata gatctgttta caagaaacac atacaggact
     1801 gatatgcctg acatggcttt ccttatcttc caaaccgtct ttgctgctac aacagcaacg
     1861 attgtttcag gtgctatggc tgagaggacc aagttcaatg cttatcttat ttacagtgca
     1921 attataacgc tcgtgattta tccggtatcc gggcattggg catggggagg tggatggctg
     1981 agccgtatgt ctgttccatt ccacgatttt gcaggttcat cagttgtcca tatggttgga
     2041 gggatggcag ccatgatggg cgctctgact ctgggaccac ggataggcaa atatgataaa
     2101 aacggaaaag ctaaagcaat cccgggtcat agtatatctc tggcaaccct cggggtattc
     2161 atcttatgga taggctggtt cggattcaat cctggttcac agcttgctgc tgcgggaaaa
     2221 gtaaatgctt cagctatatc gctgatattt gttacgacta acatatcagc ggcaggtggt
     2281 gcagttgcaa caatgttata tgtctggctg aagaacggga aacccacact tagtatgact
     2341 cttaacggtg ccctggccgg actggtagct gtaactgcag gatgtgacag cgtttcccct
     2401 ggtggagcgt tgataatcgg cttgatggca ggggtaattg ttgtcctttc cgttgaattt
     2461 tttgacaaag taattaagat agatgatccg gttggcgcaa tatcggtaca tggtatttgc
     2521 ggggcctatg gtacacttat ggttggattc ttttcaacaa gtcagggcct gttgtatgga
     2581 aatggcggcg ctttactgaa aagtcagctt attggggttg catcgatagc ctcatgggca
     2641 atctttacat catttgtact gttccgctta cttaagcttc ttaatggatt gagagtaggt
     2701 aaaagaattg aagaggaagg tctggatgta tatgaacacg gagaaaccgc atataataat
     2761 tagagaagta ttgatttgtg agctggtttg ggtttagttt aataaaaaag ggggtcgatt
     2821 aaagccccct tttttttatc tccgatattc tgttattctt cctcttcttc ttcgtggtat
     2881 gcttttataa cagcttcctg tacttctccc ggtacctgtg catactcaga gaatttcatt
     2941 gtatacattg cccggccgcc tgtaagtgaa ctgagtgcgg tggagtattt attcatctca
     3001 gcaagaggaa ctcttgcctt aataacctca aatcttttct cgcttgccat cccaagaacc
     3061 attccccggc ggccctgaag gtcgcttatt acatctccca ttctgtcgga aggtaccatt
     3121 atttcaacat cataaaccgg ctcaagtatt ttgggtgctg cattcttgaa agcctggctg
     3181 aaagcatttc ttccggcgag cctgaaagag atctcatttg aatcaaccgg atgcatcttg
     3241 ccatcataga catatacctt tatatccctt gcataagatc ctgttaacgg acccacttcc
     3301 attttttcca taatgccttt taggatcgca ggcatgaaac gtgcatcaat agatccacct
     3361 acaatgcagt tcagataaac gagtttgcct ccccattcga gatcgatctc atcctttccc
     3421 ctgactgata tctttaactc tttaccgcct atcttaagca tcgtaaggtc aggattaccc
     3481 tcaacataag gttcgattat catatgaacc tcaccaaact gaccggctcc gcctgattgt
     3541 ttcttgtgcc ggtaatcggc ctgggcagct tttgttattg tttcacggta ggggatcctc
     3601 ggaggattga agttggtcgg gatcctgtaa atattgtcaa gatgccactt cataatattg
     3661 aggtggtatt caccctgacc gtggacaatt atctgcttga gttctttgga atattcgact
     3721 ttgattgtag gatcttcaag atgaatctta tttaaggctt ctccaagttt ctcatcatca
     3781 ctttcactct gggcttttat tgctgtacgg aatttgggtt caggaaatat tacaccttca
     3841 tatctccagt cattcccggg tacattcaga gtatgatcca tcttggtgtt cttcatcttg
     3901 acaacagcac cgatatcccc gacattaagt tcagggaccc tcgtcctgtt ctttccggca
     3961 cagacatata gctgcgagag cctttctttt gtactgttgt tgctgttgat catatcaagg
     4021 ttttcagtga ctttgcctga ataaaccctg aaatagttga tctctcctat gtgttcttca
     4081 attgaagact tg
//
LOCUS       DAOSNB010000237         4073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22095_length_4073_cov_5.003733, whole genome shotgun sequence.
ACCESSION   DAOSNB010000237 DAOSNB010000000
VERSION     DAOSNB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22095_length_4073_cov_5.003733"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLG42_09060"
     CDS             <1..190
                     /locus_tag="PLG42_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31397.1"
                     /translation="NTSLTTLLTIIAIFIFGGAVIRGFVFALMIGIFVGTYSSIFVAS
                     ALMYDTSKKMKTAQPEQK"
     gene            322..483
                     /locus_tag="PLG42_09065"
                     /pseudo
     CDS             322..483
                     /locus_tag="PLG42_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            807..1466
                     /locus_tag="PLG42_09070"
     CDS             807..1466
                     /locus_tag="PLG42_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU31398.1"
                     /translation="MIKATGIRKSFGNLRVLKGIDLEVKKGEIVTIVGASGAGKTTLL
                     QILGTLDRPDEGQIFYNGQDVGKLNGKQLAAFRNKNIGFVFQFHHLLPEFTALENICI
                     PAFIAGTGRQEAMKKAMQLLDFLNLSERAEHKPSELSGGEQQRVAVARALINNPSVIL
                     ADEPSGNLDTENKKELHNLFSRLRDTFGQTFVIVTHDTQLASLSDRIIQLKDGIIVNE
                     S"
     gene            1456..2250
                     /locus_tag="PLG42_09075"
     CDS             1456..2250
                     /locus_tag="PLG42_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOU31399.1"
                     /translation="MNREKRYKGITHGEMREFLEEKYLQYNTPEFIENDPVSIPHMFS
                     GKHDREVSGFLTATIAWGRRDLILRSAHRLMELMGNSPYEFIINASENDLKRFSRFVH
                     RTFNGDDCICFLRSLMNIYRKYDSMEDIIVEGMRQDDSLVTGIASLRREFFSIPHSCH
                     AEKHFADIEGGAAGKRLFMFLRWMVRDDGRGVDFGIWKQIRPSQLYIPLDFHTGNTAR
                     KLGLLRRKQNDFTAVRELTSVLRTFDPDDPVKYDFALFGLGINERF"
     gene            2237..2995
                     /locus_tag="PLG42_09080"
     CDS             2237..2995
                     /locus_tag="PLG42_09080"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOU31400.1"
                     /translation="MKDFEFVCNKKHLQDMANDYFSFKKFTIHQDRCAFKVGTDGVLL
                     GALAEIHGNGWLLDIGTGTGLIAIMAAQRTNCRIIAIEPDYNSYIQACENVSACPWSD
                     RISVEKYTFSEYFSICDRKFDFIVTNPPYFRNSLLSPDPAKSGARHACSLSYEQLAGG
                     CARLLAEEGSLHIILPYAEGTLFVATAAEKGLFCSRIIKVKAVPSGPVIRLIMKFEKS
                     KKPLREGFLTIETGRRHEYTPEYIEATRDFYLNF"
     gene            3118..>4073
                     /locus_tag="PLG42_09085"
     CDS             3118..>4073
                     /locus_tag="PLG42_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOU31401.1"
                     /translation="MTDFQKYVDTPLMKQYYSIKAKHPDALLLFRVGDFYETFGEDAV
                     KASEILGITLTRRANGAASFVELAGFPYHALDTYLPRLVRAGQRVAICEQLEDPKLAK
                     KIVKRGITELVTPGVSLNDNVLHHKENNFLAAIHIDRRAAGVALLDISTGEFLVAEGT
                     FEYVDQILNNFQPKEVLYEKSKKDLFEENFARKFYTYKLDDWIFTHEAAYDRLIKQFD
                     TASLKGFGVENLAYGIIAAGAILYYLDITQHNQLAHINSISRIESDRHVWLDRFTIRN
                     LELFQPVFEGARTLIDVLDRTISPMGARLMKRWLSLPLKEMQP"
BASE COUNT         1271 a          737 c          916 g         1149 t
ORIGIN      
        1 aaatacctct ttgacgactc ttttaactat cattgcaatt ttcattttcg gaggtgcagt
       61 tatccgtgga tttgtttttg cccttatgat tggaatattc gttggtacat attcttcaat
      121 ttttgtggct tctgcactga tgtatgatac atcaaagaaa atgaagacag ctcagcctga
      181 gcaaaaatga aaaatgagtt atttgttttg taaagcaggg agtcttctcc cttgctttta
      241 cttttttata atgattactt gttcatggat gtcagcctgt cgaatttaat tattaatttt
      301 gcaatggtaa attgatgaaa aatgggtgta ctattattca taagcgggca ggaaatattt
      361 attataatgg tggttgtcct gttgcttttc ggagccgaca agattcctga aatagcaaaa
      421 ggaataggaa aaggaatgag ggatttcaga aaagcaactg aagaaattaa aaaagagata
      481 gaagagagta caagagaggt aagaagtgaa atatcagaaa ttcaggaatc ggtacaaact
      541 gacattaatg atatcagcga aaatatctca agaaatatag aagagtcgac cgaggcaata
      601 agaagtgatc ttaacgaagc atctgattcg cttaacaggg aagttgaaga tcttaacagg
      661 gaagttgatg acctgaattc aaatatgagc tgaaatttat accttccaca aatattcgat
      721 tattcttctt gcattagtat ttcatttttt caggcgggca atttatttca attcaaaatg
      781 ttatttttgt ctttgccaca attgatatga ttaaggctac aggtataagg aaatcattcg
      841 ggaatctcag ggttttgaaa ggaatagacc ttgaggttaa aaagggggaa attgtcacga
      901 ttgtaggggc aagcggggca ggaaagacca cattgttaca gatccttggc acactcgacc
      961 ggcccgatga aggtcagatt ttttacaacg gtcaggatgt tggaaaactt aatggaaaac
     1021 aactggctgc attcagaaac aaaaacatcg gctttgtttt tcagtttcat catctgttac
     1081 ctgaatttac agcactcgaa aatatttgta ttcctgcatt cattgctggc acgggaaggc
     1141 aggaagctat gaagaaagcc atgcagcttc ttgattttct gaatctttct gaaagagcag
     1201 agcacaagcc ttcagaactt agcggaggcg agcaacaaag agtggctgta gccagggcac
     1261 ttattaataa cccgtcggtt attcttgccg atgaaccatc cgggaacctc gacacagaaa
     1321 acaaaaagga actccataac ctcttttccc gtttgcgcga cacttttggt cagactttcg
     1381 tgattgtaac acacgacacc cagcttgctt cattgtccga cagaatcatc cagttaaaag
     1441 acggaattat tgtcaatgaa tcgtgaaaaa cgttataaag gaattacaca tggcgaaatg
     1501 cgtgaattcc ttgaagaaaa atatcttcag tataatactc cggagtttat tgaaaatgat
     1561 cctgtttcca ttccacacat gttttccggg aaacacgaca gagaagtatc aggatttctg
     1621 acggcaacaa tagcatgggg aaggcgggat ctgatattaa ggtcggcaca ccggcttatg
     1681 gaattaatgg gaaattctcc atacgaattt attattaatg catcagaaaa tgacctgaaa
     1741 agattttcac gatttgtcca cagaaccttt aatggagacg actgcatatg tttcctcagg
     1801 tcattgatga atatttacag aaagtatgac agtatggaag atattatagt tgagggtatg
     1861 aggcaggatg attcccttgt aactggtatt gcgtcgctgc gaagggagtt tttttccata
     1921 cctcatagct gtcatgctga aaaacatttt gcagatattg aaggaggagc agcagggaaa
     1981 agactattta tgttcctgcg gtggatggta agagatgacg gcaggggcgt agatttcgga
     2041 atatggaaac agataagacc ttcccagctt tacatacctc tggattttca tacggggaac
     2101 acggccagaa agcttggcct tctccggagg aaacagaacg acttcaccgc agtaagggaa
     2161 cttacatctg tgctgcgaac tttcgaccct gacgaccctg ttaagtacga ctttgctctg
     2221 ttcggtctgg gaataaatga aagattttga atttgtttgc aataaaaagc acctgcagga
     2281 tatggcaaat gattatttca gcttcaaaaa gtttacaatt catcaggaca ggtgtgcatt
     2341 taaagttggg accgacggag tattacttgg agctcttgca gaaatacatg gcaatggatg
     2401 gcttcttgat ataggaacag gaacggggct gattgctata atggctgctc agagaacaaa
     2461 ttgcaggatt attgccatag agcccgatta caactcctat attcaggctt gtgaaaatgt
     2521 atctgcctgt ccatggtcgg acagaatatc agttgagaaa tatacattca gtgagtattt
     2581 ttcaatttgt gacagaaagt ttgattttat tgtaaccaat cctccatatt tcaggaactc
     2641 cctgctgagt cccgatcctg caaaatcagg tgccaggcat gcatgctcac tttcgtacga
     2701 gcagcttgcc gggggttgcg caagactgct ggctgaagag gggagcctac atataatact
     2761 gccctacgcc gaaggcactc ttttcgttgc aactgcagca gaaaaagggc tcttttgcag
     2821 tagaattata aaagttaagg cagttccttc tggtccagtc atacgcctga ttatgaaatt
     2881 tgaaaaatca aaaaaaccac tcagggaagg ttttttaaca attgagaccg gcagaaggca
     2941 tgaatataca ccagaatata tcgaagccac aagggatttt tacctgaatt tctgaaaaga
     3001 aatagcagag gccgggtgag cggccagttc aatttatatt agcaatattc ctgtcgtaat
     3061 atatttataa atggaaggca gattgtatct ttgttaataa agatactgag cagtacgatg
     3121 actgattttc agaaatatgt ggatactcct ctgatgaagc agtattacag tatcaaggcc
     3181 aagcatcccg atgctttact gctcttcagg gtgggcgatt tttacgaaac ctttggtgaa
     3241 gatgccgtta aagcatcgga gatactgggt ataacactta cccgcagggc aaacggtgca
     3301 gcttcatttg tggagctggc agggtttccg tatcatgctc tcgacactta tttaccaagg
     3361 ctggtgagag caggacaacg tgttgcaata tgcgaacaac ttgaggatcc caaactggca
     3421 aagaaaatag taaaacgtgg tattactgaa ttggttactc ccggcgtgag ccttaatgat
     3481 aatgtgctac atcataagga aaacaatttc ctggcagcaa ttcatatcga cagaagggca
     3541 gcaggtgtgg cattgcttga tatttcaacc ggtgaatttc tggtggctga aggtactttt
     3601 gaatatgtcg atcaaatact taacaacttt cagcctaaag aggttttata tgaaaagagt
     3661 aaaaaagatc ttttcgagga aaattttgca cggaaattct atacctataa actcgacgac
     3721 tggattttta ctcatgaagc agcatatgac aggctgataa aacagtttga tactgcatca
     3781 ttaaaaggat ttggggtaga aaatcttgct tatggtataa ttgcagcagg agccatactc
     3841 tattatctcg acataaccca gcataatcaa ctggcgcata taaatagcat atcaaggata
     3901 gagagcgaca ggcatgtatg gctcgaccgt tttaccataa ggaatctcga actgttccag
     3961 cctgtttttg agggagcaag aacacttatt gatgttctcg atcgtacaat atctcctatg
     4021 ggagcccggt taatgaaacg atggctctca ctgccactga aggagatgca acc
//
LOCUS       DAOSNB010000238         4069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22123_length_4069_cov_4.411310, whole genome shotgun sequence.
ACCESSION   DAOSNB010000238 DAOSNB010000000
VERSION     DAOSNB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22123_length_4069_cov_4.411310"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..1722
                     /locus_tag="PLG42_09090"
     CDS             265..1722
                     /locus_tag="PLG42_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOU31402.1"
                     /translation="MASIKDLNPKEVWKYFHDITQIPHPSKNEKKLVEYIVKFGKEHN
                     LETIVDRAGNVIIRKPATPGMENRKGVILQTHLDMVPQKNSDKVHDFLKDPIETVVDG
                     EWVRANGTTLGSDNGIGVAAVLAILSSKSLAHGPLEALFTVDEETGMTGANGLEPGLL
                     KGEILLNLDSEIEGEFCVGCAGGVNVNAKKNYDEEKSPSGMVAYKVMVKGLKGGHSGV
                     DIALGRANSIKLLFRFLMQAEADFNIRIAMAEGGDLRNAIPREAWAIVLVPELKKYEF
                     EAFVRNYESVYRAEFAETEPDLSFNCENVAVPEKVISKDEQYKIIRAVYVCPNGVIRM
                     SQSMKGLVETSNNLAIVRCKEGKFEAYNLTRSSVDTAKESTAWRVAAAFQLIDASVVL
                     EGSYPGWKPNMKSPILKTMVNVYKSKFGEEPEVKAIHAGLECGIIGGVYPGLDMISLG
                     PTIKFPHSPDEKVHIPSVNKFFDFLCEILGEIPAK"
     gene            complement(1774..2307)
                     /locus_tag="PLG42_09095"
     CDS             complement(1774..2307)
                     /locus_tag="PLG42_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOU31403.1"
                     /translation="MTTGKPKINKLYAISSQNSKYVFSLPLKNVIKIGKRKYEIKLKH
                     DEKFGTYILWKNRKYPVEVVRSRQNKYEILFNDISYTFTIETPFGYKRLKVLSKQKGK
                     KEKEYIKAPMPGKIVDVLVREGSQVLTGEPVAVLEAMKMQNEIQSPVSGTVIKVSARP
                     NTNVVKDDILVEIKVEA"
     gene            complement(2319..3824)
                     /locus_tag="PLG42_09100"
     CDS             complement(2319..3824)
                     /locus_tag="PLG42_09100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOU31404.1"
                     /translation="MKKIKRILIANRGEIAVRIMKTARKMGIKTIAIKTAVEPSAMYL
                     SYADIVYDDTEDESEIPVFLNIDRIIEIAKKTDADAIHPGYGFLAENAYFADKCLDNG
                     IIFIGPSPDSIYKIGNKTIARKIALANKIPMAMGSQDNIANEEEAVKIAEKIGYPVII
                     KAASGGGGRGMRIVRNPEDMEKLFIIASREAEKAFNDPSVFIEKYIENPKHIEFQILG
                     DKKGNIIHLGERECSIQRKHQKLLEEAPSCALDEKLREEMGMIAVKLASAAGYYSAGT
                     VEFLLDSDRNFYFMEMNTRIQVEHPVTEEITGIDLIEQQIRIAAGEELKIKQSDVKIN
                     GWAIECRINAEDVQAGFAPDPGKIEKLSLPDEDYIRVDTGVTEGSVIVPSYDSMIAKL
                     IVKGHDRRDALKKCKLALDKVWIKGVKTTLPFFRMLIRHPKFIDGSFTTSFIDELNQY
                     YYNSEYEEMIAAWLATKLFIEENLSEDSIKLDFSESREINPWLLNKRLSQF"
BASE COUNT         1164 a          820 c          753 g         1332 t
ORIGIN      
        1 acatgcaaaa gaaaatagtt tttcattaat tccaaaagta aagtatttct aaaattctca
       61 agtcattaaa attgttaaga ttttgttaca aataagagtg ataaaaaatt tactcatatt
      121 aaatatatta ttctggctct aatggaaagc cttataaaac aattttatag tattatgatt
      181 ttttgataat ttgtctttcg ggtaattttt atatatttaa ccacacttaa tatttatctt
      241 tgaaaaaact aataatttga tattatggcc agcattaaag atctaaatcc gaaggaggta
      301 tggaaatatt ttcatgacat tacacaaatt ccacatcctt caaagaatga aaagaaactt
      361 gttgaatata tagtcaaatt tggcaaggaa cataacctcg aaacaatagt tgacagagcc
      421 ggtaatgtca taatccgtaa gcctgcaaca ccaggtatgg aaaacaggaa aggagtaata
      481 ctccagaccc atctcgacat ggtgcctcag aaaaacagcg ataaagtaca tgattttctg
      541 aaggacccga ttgaaactgt tgttgacgga gagtgggtga gggcaaatgg aaccacactt
      601 ggctctgaca atggaatagg agttgctgct gttctggcaa tcctttcatc aaaatcgttg
      661 gctcacggac ctcttgaggc actctttact gttgacgaag aaacaggaat gacaggtgct
      721 aacggacttg aaccgggttt gcttaaagga gaaattctgc ttaatctcga ttcggaaatc
      781 gaaggtgaat tttgtgttgg ctgcgctggc ggcgtaaacg tgaacgcaaa aaagaattac
      841 gacgaagaaa agtctccttc aggaatggtt gcatacaaag taatggtcaa aggtcttaag
      901 ggagggcatt cgggagttga tattgcactt ggccgcgcaa attcaattaa gcttctcttc
      961 cggttcctta tgcaggccga ggcagatttt aatatcagaa ttgccatggc agagggaggc
     1021 gacctcagaa acgctatacc acgcgaagca tgggcaattg tactcgttcc tgaacttaag
     1081 aaatacgagt ttgaagcttt cgtaaggaat tacgaatcag tttacagggc tgaatttgca
     1141 gaaactgaac cagatctctc atttaactgt gaaaatgttg ctgttcctga aaaagtaatc
     1201 agtaaagatg aacaatataa aataatcaga gctgtttatg tatgcccaaa tggcgtaata
     1261 cgtatgagcc agtcgatgaa aggtcttgtc gagacctcaa ataaccttgc aatagtccgt
     1321 tgcaaggagg ggaagttcga agcatataac cttacaagaa gttctgttga tactgccaaa
     1381 gaatcaacag catggcgtgt ggcagctgca tttcagctaa ttgatgccag tgtagtactc
     1441 gagggctcat atccgggatg gaaacctaac atgaaatcac cgatcctgaa aacaatggtt
     1501 aatgtatata agtcaaaatt cggtgaagag ccagaagtaa aagcaattca tgctggactc
     1561 gaatgtggaa ttataggtgg agtgtatccg ggccttgata tgatttcgct ggggccaaca
     1621 ataaaatttc ctcactcacc tgatgaaaaa gtacatatcc catcagttaa taaattcttt
     1681 gattttctgt gcgaaatact cggggaaata cctgctaaat aagaatataa agagaataaa
     1741 tctgataatt gtaaaagtgg aagcctcaat tcttcaggct tctactttta tttcaaccag
     1801 tatatcatct ttaacaacat tggtatttgg acgggcactt actttgatta cagtgccact
     1861 tactggcgac tgaatttcgt tttgcatctt cattgcctcc agaactgcta caggctctcc
     1921 ggtgagcacc tggctaccct cccttaccag cacatcaaca atcttgcccg gcataggagc
     1981 ttttatgtac tctttctctt ttttaccctt ctgcttgctc agtaccttaa gtcgtttata
     2041 gccaaatgga gtttcaattg taaatgtgta actgatgtca ttaaacagaa tctcgtattt
     2101 attctgcctc gaccttacaa cttctacagg atattttctg tttttccata atatgtatgt
     2161 gccgaacttt tcatcatgct tcagttttat ttcatattta cgcttgccta tttttatgac
     2221 gttttttagg ggaagcgaaa aaacatactt gctgttctgt gatgaaatgg cgtaaagttt
     2281 gttgattttc ggtttaccgg ttgtcataat cttcaaaatt agaactgact tagcctttta
     2341 ttaagaagcc agggatttat ttcccggctt tcactgaaat caagttttat actgtcttca
     2401 ctcagatttt cttctataaa caatttggta gcaagccatg ctgctatcat ttcctcatat
     2461 tcactattat agtaatattg gttcagttca tctataaagg aagttgtgaa actgccatct
     2521 atgaatttcg gatgccttat cagcattctg aagaagggaa gtgttgtctt gacccctttt
     2581 atccagactt tatcaagagc aagtttacat tttttgagcg catccctgcg gtcatgcccc
     2641 ttaacaataa gctttgcaat cattgagtca taacttggta caattaccga accttctgta
     2701 actccggtat cgactctaat ataatcttcg tcgggcagac ttaatttttc tattttaccg
     2761 ggatccgggg caaaacctgc ctgaacatct tcagcattta tcctgcattc tatggcccag
     2821 ccattgattt taacgtcgct ctgtttaatt ttcaactctt cgccagccgc aatccttatc
     2881 tgctgctcta taaggtcaat tcctgtaatt tcttctgtta ccggatgttc aacctgtatt
     2941 ctggtattca tctccatgaa atagaaattc ctgtctgaat cgagcagaaa ttcaactgtg
     3001 cctgccgagt aatacccggc agcacttgca agctttactg cgatcatccc catttcttcc
     3061 ctgagttttt cgtctaatgc acatgatggg gcttcctcaa gaagcttctg atgctttcgt
     3121 tgaatggaac actccctttc acctagatgg ataatatttc ctttcttatc gccgagtatc
     3181 tgaaattcaa tatgtttagg attttctata tacttttcga tgaagacaga agggtcgttg
     3241 aatgcttttt cagcttcccg gctcgctatg atgaacaatt tttccatgtc ctcggggttc
     3301 ctcacaattc tcattcccct tccgccacct cctgatgctg ctttaataat aaccggataa
     3361 cctatttttt cggcaatttt tacagcttct tcctcattgg ctatattatc ctggctgccc
     3421 atagccatcg gtattttgtt ggcaagagct attttacgag caattgtctt atttccaatc
     3481 ttatatatcg aatcaggaga agggcctata aatattatgc cgttatccag acatttatct
     3541 gcgaagtatg cattctctgc aagaaatccg taacctggat gaatggcatc tgcatctgtc
     3601 ttctttgcta tttcaattat tctatcaatg ttgagaaaaa caggtatctc cgattcatct
     3661 tcagtatcat cataaacaat atctgcatat gagaggtaca tagccgaagg ttcgacagca
     3721 gttttaattg caattgtttt gatgcccatt ttcctcgcag tcttcattat cctgaccgct
     3781 atctcgcccc tgtttgctat aagaattctc tttattttct tcatatttta acttaatctt
     3841 tttgttatac ggagagcata gcttccagtc gatatgttta ttgtttataa cttaaaaaac
     3901 aattatatat ttcagccatt ggttccatct gacctttgtc tcttacctat ctccttttgc
     3961 ctttataatg gcatattgcc atgttttctg gagggcacct taacccattt attccttaga
     4021 gcttcgaatg ctgatattat cttttttctt gtcacagccg ggtcaatta
//
LOCUS       DAOSNB010000239         4067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22136_length_4067_cov_3.531904, whole genome shotgun sequence.
ACCESSION   DAOSNB010000239 DAOSNB010000000
VERSION     DAOSNB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22136_length_4067_cov_3.531904"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PLG42_09105"
     CDS             complement(<1..939)
                     /locus_tag="PLG42_09105"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU31405.1"
                     /translation="MDYYFIYRKEWDKKLEGLLESHNIFAPVPNEFSLDYEVLTPSLI
                     PLVSYNKPKPASPLKVFFTPIKENVTSPGKDEKPIIIMGVPNCDVEALGILDEIYLDE
                     SFEDLFYRKRRQKTLIISTDCFDVQEHCHCTSYGVKPWSEQRADLAVALIKDQIILRV
                     ISDKGKTFVTETIQASPLNDKGLISSIENRHREIEDLLNRTNKELPDYKETGRLVKSS
                     SKAIWVKHSSTCVSCGACTTICPTCTCFLLIDKPGFHKVKQMDACQYPGFEKTAGGED
                     PLKELHERFRNRYMCKYVWKPEKFKHIACTGCGRCIE"
     gene            1127..1807
                     /locus_tag="PLG42_09110"
     CDS             1127..1807
                     /locus_tag="PLG42_09110"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU31406.1"
                     /translation="MKKFTKTLIKNSLRRIFLYRACLNRLKMMGMEKVFSYTLAMEAG
                     VSPDQVRKDFSEFNIKGNKRGGYNIDELLEKMENIFYKNREHNIVLVGMGNLGLALSK
                     YSRFLQRNMNIVATFDIDPFKQKARTEIPVYGMDRLKEIIQRFKVKVAIIAVPEISAQ
                     QVCDDLVKAGVKGIVNFAPVLLKTPPNVIVNNVNLCDELESVIYYVHHQLKIEGLKNI
                     ELLYGDMK"
     gene            1807..>4067
                     /gene="hypF"
                     /locus_tag="PLG42_09115"
     CDS             1807..>4067
                     /gene="hypF"
                     /locus_tag="PLG42_09115"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOU31407.1"
                     /translation="MVKGLVQGVGFRPFIYRLANSLNLKGMVDNRTDGVLIIVEGPEE
                     KITEFQEKIVKLAPQASQIKSIEVKHRELSGFADFRIAPSINGGARITEISPDIAVCS
                     ECLSDMVNDPLRIDYPFVNCTNCGPRFTIIESLPYDRQTTSMKYFSMCSKCESEYHNI
                     IDRRFHAQPVACNRCGPQYLYEGPEGRIENISDILLTVADRIHRGETVAVKGQGGYHL
                     VCNALDAAAVVQLRQRKHRDAKPFAVMFRDTAALLDFCHANNRELEELTSWRRPVVIL
                     RQKKELPPEVNSNLGTIGAILPYMPFHYLLFRHLNTPAIVFTSGNISEEPVIKDDDTA
                     RKILLPVAGALLSYNREIVNRADDSVIRIAADKPRIIRRARGYVPSPVDLDFDAEGVI
                     ALGAEQKNCFCIGKGEQAIMSQHIGDLKNMPAYDFFRESINRFRAMFLFEPQIIACDL
                     HPDYLSSVYAGELQKEYNIPVIKVQHHHAHIASCLAENHLDEKVIGISFDGTGYGTDG
                     NIWGGEFLVADIREFVRYTHFDYIPLPGGDKVADEPWRTAFSYLYKYFGNSIDYERIP
                     AFRSIGVKKMEVVREMIDKNINTPLSSAAGRLFDAVSALLGLCTVSSFDSEAPMRLES
                     AIKETTDDYFPFEMAETLSFATAIKAILEESEKNELSYISAKFHNTVARASLEVAKKI
                     RTEMSINKVVLSGGVFQNKYLLEKTSELLSEAGFDVFTNMLVPANDGGIALGQLAVAS
                     KFKLYVPESSSKS"
BASE COUNT         1261 a          817 c          866 g         1123 t
ORIGIN      
        1 ttcaatgcag cggccacagc ctgtacaggc gatgtgtttg aacttttcag gtttccagac
       61 atacttgcac atatatctgt ttctgaaacg ctcgtgcagt tctttcagcg ggtcttcacc
      121 tccggcagtc ttttcaaatc ccggatactg gcatgcatcc atctgtttta ccttgtggaa
      181 gccgggttta tcaatcagca gaaaacaggt gcaggttgga catatcgttg tacaggctcc
      241 gcacgaaaca caggtggatg agtgctttac ccatattgct tttgaagaag attttacaag
      301 tcgacctgtt tctttatagt cgggaagctc tttgtttgtg cggttgagga gatcttcaat
      361 ctctctgtga cggttttcta tgctggaaat cagacctttg tcgttaagag gagaagcctg
      421 aatggtttca gtaacaaatg ttttgccctt atctgatata accctcagta taatctggtc
      481 cttaatcaac gcaacagcaa ggtcggccct ctgttcactc catggtttga ctccgtaaga
      541 ggtgcaatga cagtgctcct gtacatcaaa gcagtcggta cttattatca gagtcttctg
      601 cctgcgtttt ctgtaaaata aatcttcaaa actttcatca aggtagattt catctagaat
      661 gccaagcgct tccacatcac agttaggtac acccattatt attatgggtt tttcatcttt
      721 ccccggagaa gttacatttt ccttaatagg tgtgaaaaag actttaagcg gagaggccgg
      781 ttttggtttg ttatatgaaa caagagggat caacgaaggc gtgagcactt cataatccag
      841 actgaattca tttggtaccg gggcaaaaat attatgggat tcaagcagtc cttccagctt
      901 tttatcccac tcttttctgt aaatgaagta ataatccatt tatgtgattt agttcacata
      961 taaaagtaaa aaatatgaaa atacattcga ataattttaa taaaaaaatt atgtgaaaaa
     1021 aatcacaaaa aatataaata tctaaaaatc aataaaaaat aaattattgt aaattaaaaa
     1081 aattttttta acttgcatca ggaataattt aatagacata cttaatgtga aaaaattcac
     1141 aaaaacactg ataaagaaca gtctcagaag gatatttcta tacagggcat gtctcaacag
     1201 attaaagatg atggggatgg aaaaggtctt ttcttatacc ctcgctatgg aagcaggagt
     1261 gtcgcccgac caggtaagaa aagacttctc tgaattcaat attaaaggaa acaaacgagg
     1321 aggatataat atagatgaac tcctcgaaaa gatggaaaac attttttata aaaacaggga
     1381 gcataatatt gtgctggtag gaatgggtaa tctcggtctg gcactttcga aatatagcag
     1441 gtttcttcaa agaaatatga atatcgttgc aaccttcgat atcgacccat tcaaacagaa
     1501 agcccgtaca gaaatacccg tctacggaat ggacaggctt aaagaaataa tacaacgctt
     1561 taaggtaaag gttgccataa tcgccgttcc cgaaatatca gcacagcagg tgtgcgacga
     1621 ccttgtaaaa gcgggagtaa agggaatagt aaactttgct ccagtcctgc ttaagacacc
     1681 tcccaatgta attgtaaata acgttaacct gtgcgatgaa cttgaaagcg ttatctatta
     1741 cgttcaccac cagctgaaga ttgaaggact caaaaacatc gaactattat acggggatat
     1801 gaaataatgg ttaagggact cgtacagggt gtcgggttca gaccttttat ctaccgcctt
     1861 gcaaattccc ttaaccttaa gggaatggtt gataacagga ctgatggagt actgattatt
     1921 gttgaaggtc ctgaagaaaa gattacagaa ttccaggaaa aaatagtaaa acttgcacct
     1981 caggcttctc agattaaatc catcgaagtt aagcatagag aactttcagg ctttgctgat
     2041 ttccggatag ccccaagcat caacggtggc gcccggataa cagaaataag tcctgatatt
     2101 gcagtttgct ccgaatgcct ttcggatatg gtcaatgacc ccttacgcat agattatccc
     2161 tttgtaaact gtaccaactg cggtccgagg ttcacaatta ttgagagtct gccttacgac
     2221 aggcagacca cttcaatgaa gtatttcagc atgtgttcaa agtgtgagtc ggaataccac
     2281 aatatcatcg acaggaggtt tcatgcccag cctgttgcgt gcaatcgttg tgggccgcaa
     2341 tatctatatg agggtcctga gggaagaata gaaaacatca gtgatattct cttaacagtt
     2401 gcagatagga tacacagggg cgaaactgtc gcagtgaagg gacagggagg ataccatctt
     2461 gtttgtaatg cccttgacgc tgcagcagta gtacaactga ggcaacgcaa acaccgcgac
     2521 gcgaaacctt ttgcagtgat gttcagggac acggcagcac ttctggattt ctgccatgcc
     2581 aataatagag aacttgagga actaacatcg tggcgcaggc ctgtggtcat tctgcggcag
     2641 aaaaaagaat taccacccga ggtcaacagt aaccttggca caataggtgc tatacttcca
     2701 tatatgccgt ttcattatct gctgttcaga catcttaata ctccggcaat agtctttaca
     2761 agtggaaata tctcagaaga acctgtgata aaggatgatg atactgcccg gaagatcctt
     2821 ttgcccgttg ccggcgcact cctttcgtac aacagggaaa ttgtaaaccg tgccgatgat
     2881 tctgtgatac gtattgcagc tgacaaaccc cggatcatca ggcgcgcaag gggatacgtt
     2941 ccatcacctg ttgaccttga tttcgatgcc gaaggcgtca ttgcactggg cgcagagcaa
     3001 aaaaactgct tctgcatcgg gaaaggagag caggcaataa tgagccagca tatcggcgac
     3061 ctgaaaaaca tgcctgctta tgattttttc agggaatcaa taaatagatt ccgtgctatg
     3121 ttcctgtttg aaccacagat tatcgcatgc gaccttcatc ccgattacct ttcgtcagtg
     3181 tacgccggag aacttcaaaa agaatacaat attcctgtaa taaaagttca gcaccatcat
     3241 gcacatattg cctcatgcct tgcagaaaat catctcgatg agaaagttat aggcatcagc
     3301 ttcgatggaa caggctacgg aaccgatgga aatatttggg gtggagagtt tcttgtggct
     3361 gacatcagag aatttgtcag gtacactcac tttgactata tacccctgcc gggaggagat
     3421 aaagtggcag atgagccatg gcgaacagcc ttttcatatc tttataaata ttttggcaat
     3481 agtattgatt acgaaagaat tcctgccttc aggtcaatcg gagtaaaaaa aatggaagtt
     3541 gtaagggaaa tgatagataa gaatattaat acgcctctta gctcagctgc tggccggctc
     3601 tttgatgctg tttctgcatt gctcggactc tgcacggtct cttccttcga ctctgaagct
     3661 ccaatgaggc tggaatcagc cataaaagaa acaacagatg attattttcc ttttgaaatg
     3721 gccgaaacct taagtttcgc cactgcaata aaagcaatac ttgaagaatc tgaaaaaaat
     3781 gaattatcgt atatttcggc aaaatttcac aatacagttg caagagcttc acttgaggtg
     3841 gcaaaaaaga taagaacaga aatgtcaatt aataaggtag tgctttccgg aggtgttttt
     3901 cagaataaat atctcctcga aaaaacttca gaactcctct cagaagcagg attcgatgtc
     3961 tttacaaata tgctcgttcc tgccaatgat ggtggtatag ctcttggaca gcttgcagtt
     4021 gcatcaaaat ttaaattata tgtgcctgag tcttccagca aaagtta
//
LOCUS       DAOSNB010000240         4052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22246_length_4052_cov_4.152614, whole genome shotgun sequence.
ACCESSION   DAOSNB010000240 DAOSNB010000000
VERSION     DAOSNB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22246_length_4052_cov_4.152614"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLG42_09120"
     CDS             complement(<1..183)
                     /locus_tag="PLG42_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOU31408.1"
                     /translation="MKLIDTHTHIYLPEFDSDRDNVVERAVCKGVKIMLMPNIDVCSL
                     KPMIEASKRYKGICLPM"
     gene            complement(274..450)
                     /locus_tag="PLG42_09125"
     CDS             complement(274..450)
                     /locus_tag="PLG42_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31409.1"
                     /translation="MSCTADEVDKRLKTIMKTIHDTCVKFGRKDNDCINYVDGANIAG
                     FVKVVDAMIAQGLV"
     gene            complement(458..889)
                     /locus_tag="PLG42_09130"
                     /pseudo
     CDS             complement(458..889)
                     /locus_tag="PLG42_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245618.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
     gene            complement(1051..2178)
                     /gene="dprA"
                     /locus_tag="PLG42_09135"
     CDS             complement(1051..2178)
                     /gene="dprA"
                     /locus_tag="PLG42_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOU31410.1"
                     /translation="MSFPCAMSEVPLKYKIALSLIPRIGDINARKLVSYIGSVEGVFT
                     EPYRSLIKIPGIGANLARYISERSYLSIAEKEAEYVEKNGIKTFFYLDNDYPYRLRQC
                     EDSPVLFYFKGNCDLNAQRILSVVGTRNATSRGKEICEKIITGLAFDNPELVIVSGLA
                     YGIDITAHKSAMANNLPTIGVLGHGFKTTYPSVHKPVANAMLKNGGLLTDFRSDELPE
                     RNNFIKRNRIIAGISDATLIVESAITGGALITADIANSYNRDVFAIPGRIDDIWSAGC
                     NNLIKRNKAALVESHEDIEYFLNWLPAKTKQPVQRILFTELTEDEKRIFELINKEEEI
                     NIDQICRETGMPIHKLSNILLQMEFNGLIKSYPGNIYKIVY"
     gene            complement(2271..3608)
                     /gene="gdhA"
                     /locus_tag="PLG42_09140"
     CDS             complement(2271..3608)
                     /gene="gdhA"
                     /locus_tag="PLG42_09140"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOU31411.1"
                     /translation="MNVEKIMSELTKKHPGEVEYLQAVREVLESIEDVYNENPQFESA
                     RIIERLVEPDRIIAFKVPWVDDEGDVHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGAKGGSDFNPKGRSNAEIMRFCQSFMLELWRHIGPEIDV
                     PAGDIGVGGREIGYLYGMYKKLTTEHTGVLTGKGINWGGSLIRPEATGFGVVYFTSEM
                     LATRGESLKGKTVAVSGFGNVAWGAVTKVNELGGKVVTLSGPDGYIYDPEGVSGKKID
                     YMLELRASNQDIVKPYASEFEGVEFHEGKRPWEVKVDIALPCATQNELDARDAESLVK
                     NGCICVCEGANMPCTPEAVEIFQKNGLLFAPGKAANAGGVATSGLEMSQNSMKLSWGR
                     EEVDSRLREIMHNIHEQCVKYGKKPDGYIDYVKGANVAGFLKVAHAMLDMGVV"
     gene            complement(3695..>4052)
                     /locus_tag="PLG42_09145"
     CDS             complement(3695..>4052)
                     /locus_tag="PLG42_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31412.1"
                     /translation="TIILTFLYFCYLIYPMVLNYQYIYYSDEDDKIVFRYFFAGIIGG
                     RRNSIEIHKSQYAGYRREKKLFGLIQSVILFQYVKGGIAKYPPVYISLLNSEERAKVL
                     YSLYKNAPASATEIKE"
BASE COUNT         1146 a          857 c          722 g         1327 t
ORIGIN      
        1 catcggaagg caaattcctt tatatctctt cgatgcttca atcattggtt taagggagca
       61 gacatcaata tttggcatca acataatctt aacgccttta catacagccc tttctacaac
      121 attatcgcga tcggaatcga attcaggcag gtaaatatgt gtatgtgtat caataagttt
      181 cattttttct gaaagcaagc agcaaagata taaaaaaaga ggccctttaa agagcctctt
      241 tataaaatct ataaattgat tcaacaagct ggatcagaca agtccctggg ctatcattgc
      301 atcaactact ttgacaaaac ctgctatatt ggctccatca acatagttta tgcagtcgtt
      361 gtcttttctc ccgaatttaa cacaagtatc gtgtatagtt ttcattattg ttttcagtct
      421 tttgtcaact tcatcggctg tacatgacat tctctgggag ttctgtcata aagctctggc
      481 agaatctcat tacttcatta tttgattttc cttctgggtt gaagtcggag ccaccttttg
      541 cacctcccac agggagtgtg gtgaggctat tcttaaatac ctgttcaaaa gccagaaact
      601 tcattatact gagtgtgact gatggatgaa atcttaagcc tcctttatag ggacctatgg
      661 cactgttcat ttctacgcga aatcctctgt ttatctgtat attaccttcg tcgtcgagcc
      721 atggtactct gaaaattact attctttcgg gttctgttat cctttcaagt atcttgaatt
      781 gcctgtaatg aagattctct taaataaaag gcattttaga cttaaccacc tcctgtactg
      841 cctgatgaag ttcgctttca ccagggttct tctttttgat ttcattcatg aatctttcaa
      901 cttcctgata tatattaccg atttttaaga gtttgaaaat ttaactttct taataagatt
      961 aatttttagg gggtagaaat aataaaaatt taatattaca ataaaattat agaaaaaaat
     1021 taaaaatttt ttgattagga agatgaattg ttaataaacg attttataaa tatttccggg
     1081 gtagctttta ataagtccgt taaattccat ttgtaagagg atatttgaaa gtttatgtat
     1141 tggcatgcct gtttctctgc aaatctggtc gatatttatt tcctcttctt tattgatcaa
     1201 ttcaaagatt cttttttcat cttcggtaag ttctgtaaaa agaatgcgct ggaccggttg
     1261 ttttgttttt gccgggagcc agttgagaaa gtattcgata tcttcatggc tttctaccag
     1321 tgcagcttta tttctcttta tcagattatt gcatcctgct gaccatatgt catcaatacg
     1381 tccgggaatg gcaaaaacat cacggttata tgaatttgca atatcggctg ttataagtgc
     1441 tcctccagta attgccgatt ctacaataag tgtggcatcg gatatacctg caataatccg
     1501 gtttcttttt atgaagttgt ttctttcagg aagttcatca gaacggaaat ctgtcagcaa
     1561 tccgccattc tttagcatag catttgccac aggcttatga actgaggggt atgtagtttt
     1621 aaatccgtga cccaatacac caatagtggg gaggttattt gccatagcac ttttatgcgc
     1681 ggtaatgtca ataccgtagg caagtccact tactattact aattccgggt tatcaaaagc
     1741 gaggcccgta atgatttttt cgcatatttc cttacccctt gaagttgcat tgcgcgtacc
     1801 aaccacactg agtattcttt gtgcgttgag atcgcagtta cctttaaaat aaaaaagaac
     1861 cggagagtct tcacattgcc tgagccggta aggatagtca ttgtcgaggt agaaaaatgt
     1921 ctttatacca tttttttcta catattctgc ttctttctcc gcaatgctga gataagaacg
     1981 ttcactaata tatcttgcaa gattggctcc tataccaggt atttttatca gtgaacggta
     2041 tggttctgtg aaaacacctt ctacactgcc gatgtatgaa accagtttac gggcattgat
     2101 atctccgatg cgcggaataa gactcaatgc aattttatac ttgagaggca cttctgacat
     2161 agcacaagga aatgacatgc aagaacaaat atattaaaaa taaacagact cacaaaactt
     2221 gtgagtctgt ttatataaga catttaattt cagatattta tatgttgttt ttaaacaaca
     2281 cccatatcga gcattgcatg tgcgacttta aggaaacctg ccacatttgc tcccttcacg
     2341 taatcgatat aaccgtcggg ttttttcccg tacttaacac actgttcatg aatattatgc
     2401 attatttcac gcagtctact gtcaacttct tctcttcccc agctgagttt catagagttt
     2461 tgtgacattt cgagtccaga tgttgccacc ccgcctgcat ttgctgcttt accgggagcg
     2521 aacagaagac cgtttttctg gaatatctca actgcttcgg gggtgcaggg catatttgct
     2581 ccttcacaaa cgcagatgca tccgtttttt acaaggcttt ctgcatctct ggcatccagt
     2641 tcattctgtg ttgcacaggg cagggctata tcaaccttta cttcccatgg acgtttcccc
     2701 tcatgaaatt caacaccttc aaattcactg gcataaggtt taactatatc ctggttcgaa
     2761 gccctcagtt cgagcatata gtcaattttt ttgcccgaaa ctccctccgg gtcgtaaata
     2821 taaccatctg gccccgatag agtgactact tttcctccca gttcgttcac ctttgtaaca
     2881 gcaccccatg caacatttcc aaaaccggaa acagctactg ttttgccttt taatgactcg
     2941 ccccttgtag caagcatttc gcttgtaaaa tataccacac cgaatccggt tgcctcaggt
     3001 cgtattaaag aaccacccca gttgatccct ttgcccgtaa gcacgccagt gtgttcagtg
     3061 gtaagctttt tatacattcc atacaggtat ccgatttcgc gtccacccac accaatatcg
     3121 ccagcaggga catcaatttc cggcccgatg tgtctccaca gttcgagcat aaacgactgg
     3181 cagaaacgca taatttcagc atttgacctt cccttgggat tgaagtcaga tcctcccttg
     3241 gcacctccca taggaagcgt ggtgagtgag tttttgaata tctgctcaaa cccaagaaat
     3301 ttaagaattg aaagattgac actcgggtga aatctcaaac cacccttgta gggtcctatg
     3361 gcattattaa attgcactct gtaacccagg ttcacatgaa catctccttc atcatcaacc
     3421 catggaactt taaaagcaat aatacggtcg ggttccacaa gccgctcgat aatccttgcc
     3481 gattcaaact gggggttctc attgtacaca tcctcaattg attcgagaac ttcccttaca
     3541 gcctgaaggt attcaacctc accgggatgc ttttttgtga gctcactcat tattttttcg
     3601 acattcataa tatagagtat taagttattt caaaaataat tgcctgtgcc aaaggttgat
     3661 aaggttttga aaattaaaaa tgattttact catactattc ctttatttcg gttgcacttg
     3721 caggagcatt tttataaagt gagtaaagga cttttgctct ttcttcggaa ttaagaaggc
     3781 tgatgtaaac aggagggtac tttgcaatgc cccctttgac atattgaaaa agtattacac
     3841 tctgaatcag accaaaaagc tttttttcac gcctgtaacc tgcatattgt gatttgtgaa
     3901 tttcaattga atttcgtctt ccaccaataa ttcctgcaaa gaaatacctg aaaacaattt
     3961 tgtcatcttc atccgaataa taaatatact ggtaattaag aaccatcgga tagatcaaat
     4021 aacagaagta cagaaaggta aggattatag tc
//
LOCUS       DAOSNB010000241         4025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22475_length_4025_cov_4.655416, whole genome shotgun sequence.
ACCESSION   DAOSNB010000241 DAOSNB010000000
VERSION     DAOSNB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22475_length_4025_cov_4.655416"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /gene="sufD"
                     /locus_tag="PLG42_09150"
     CDS             complement(<1..1289)
                     /gene="sufD"
                     /locus_tag="PLG42_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOU31413.1"
                     /translation="MKPRNELTEKFYRLYRENLPAIESVSASLLNRSREEAIEKFMQS
                     GIPTRKTEAYRYTPLETYFGYEYHNYIPASTDFERVRRFKCDVEDLDCYSIILHNGYF
                     PESGVKHITLPGNIWLGSLNEAAEKFKSVVENHLGKYSGREDDGLIHLNTAMSPGGLF
                     VHIPAGAIVEKPVQIINLVDSEQDIFNQKRNLIIVEPGSSITLIVCDHTISLNRFLTN
                     TVTEVYVGENSHFNIIRVQNEHNNSFKITSTFIYQEKNSVVSSNNITLHGGMVRNNTY
                     HYLAGEGAQCSSYGLYLADKMQHVDNYVNVEHLAPGCTSNQLFKGVLDEMARGVFNGR
                     IYVEKGAQGTSAYQKNNSIILTDDARMYTRPHLEIFADDVKCSHGATVGQLDEDAMFY
                     LQSRGINKNEAKLMLMAAFADEVIQKINIGPLRQRIIG"
     gene            complement(1289..2050)
                     /gene="sufC"
                     /locus_tag="PLG42_09155"
     CDS             complement(1289..2050)
                     /gene="sufC"
                     /locus_tag="PLG42_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOU31414.1"
                     /translation="MLVIKNLRATVGGREILKGISLEVKEGEIHAIMGPNGSGKSTFA
                     AVIAGRDYATVTEGTVTYLGRNLLEMSPDERALEGIFLGFQYPVEIPGVSMTNFLKTA
                     VNEQRKHRGLEPLSASDFLKMMREKKELVGLDSKLDNRSVNEGFSGGEKKKNEIFQMA
                     MLEPRLAILDETDSGLDIDALKVVANGVNKLRRPDNAFIVITHYQRLLDYIIPDVVHV
                     LYDGRIVMTSGRELAIELEQKGYEWIKREVEKSGI"
     gene            complement(2068..3519)
                     /gene="sufB"
                     /locus_tag="PLG42_09160"
     CDS             complement(2068..3519)
                     /gene="sufB"
                     /locus_tag="PLG42_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOU31415.1"
                     /translation="MPEGKKKKNIIDEVTGSEYKYGFYTDIEAEKVPPGLNEDIIRLI
                     SEKKNEPEWLLEFRLKSYRHWLTMEMPEWANLKIPEINYQDIVYYSAPAVRKKKSSAE
                     IDPELKKTFDRLGIPLDEQKILSGVAVDAVMDSVSVKTTFRETLAELGIIFCSFSEAV
                     REYPDLVKKYLGTVVPYSDNYFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAAK
                     TGQFERTLIVADDESYVSYLEGCTAPIRDENQLHAAVVEIIAEKDAEVKYSTVQNWYP
                     GDHEGRGGIFNFVTKRGMCRGENSKISWTQVETGSAITWKYPSCVLKGDNSTGEFYSV
                     AVTNHFQQADTGTKMIHIGKNTRSTIVSKGISAGRSQNSYRGLVKVLRNARNARNYSR
                     CDSLLLGDKCGAHTFPYIEVENPTAIVEHEATTSKVGEDQIFYCNQRGISTEDAVSLI
                     VNGYTREIINKLPMEFAVEAQKLLQITLEGSIG"
     gene            3843..>4025
                     /locus_tag="PLG42_09165"
     CDS             3843..>4025
                     /locus_tag="PLG42_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31416.1"
                     /translation="MLIIPISLFASSPGEVPSIGPIRVEFIIFGMILAGVALFHKQTF
                     WVAVTGLTVLLIFKFIF"
BASE COUNT         1071 a          890 c          741 g         1323 t
ORIGIN      
        1 ccgataattc tttgcctgag cggtccgatg tttattttct ggatgacctc atccgcaaag
       61 gcagccataa gcatgagttt tgcctcgttt ttatttattc ccctcgattg caggtaaaac
      121 atggcgtctt catccagctg tcccacagtg gcaccatggc tgcacttaac atcatcagca
      181 aatatctcta aatgcggccg tgtgtacatt ctggcatcat ctgtcaaaat aatggaattg
      241 tttttctggt aagccgatgt accctgtgct cccttctcaa cgtaaattct tccgttgaaa
      301 acccctctag ccatttcatc cagtacacct ttaaagagct gattgcttgt acagcccggg
      361 gcaaggtgct caacattaac atagttgtcg acatgctgca ttttatcagc aaggtatagt
      421 ccgtaagatg aacattgagc tccttcacca gcaagataat gataagtatt gtttcttacc
      481 attccaccgt gaagggttat gttatttgaa cttacaactg aattcttttc ctgataaata
      541 aatgtgctcg ttattttgaa ggaattatta tgttcattct gtacccttat tatattgaaa
      601 tgtgaatttt cgcctacgta tacttcagtt actgtattgg taaggaatct attgagtgag
      661 atggtatggt cgcacacaat aagggttata gatgaacccg gctccactat aatcagattt
      721 cttttctgat tgaaaatatc ctgttcagaa tcaacaagat ttattatctg aaccggtttt
      781 tcaacaatag cacctgccgg tatatgtaca aagagacctc cgggtgacat ggcagtattg
      841 agatgtatca gcccgtcatc ttcccttccg ctgtatttcc cgaggtggtt ctccacaaca
      901 gatttgaatt tttcagcagc ttcattaagg ctgccaagcc agatattgcc aggtaaagta
      961 atgtgtttca ccccggattc aggaaaatat ccgttatgaa gaattatgct atagcagtca
     1021 aggtcttcaa cgtcgcattt aaaccttctg actctttcaa aatcggtcga tgcaggaata
     1081 taattgtgat attcatatcc gaaataagtc tccaatgggg tatacctgta tgcttcagtt
     1141 ttccgtgtag gtataccgga ttgcataaac ttctcaattg cttcttccct tgaacggtta
     1201 agcagagaag ctgaaacaga ttctattgca ggaaggtttt ccctgtaaag cctgtaaaat
     1261 ttttcagtca gttcgttacg gggtttcatt atattccgct tttctcaact tctctcttta
     1321 tccactcgta tcccttttgt tcaagttcaa tagcaagttc cctgcctgat gtcataacta
     1381 tcctgccatc atacaggaca tgcacaacat caggtataat ataatcaagg agtctctggt
     1441 aatgagtaat aacaataaag gcattatcgg gcctgcgaag tttgttaacc ccgttagcaa
     1501 caactttaag ggcatcaata tcaaggcctg aatcggtttc atcgagtatt gccagccggg
     1561 gctcgagcat tgccatctgg aagatttcgt ttttcttttt ctctcctccc gaaaaacctt
     1621 catttaccga acggttgtcg agtttggaat cgagtcctac cagttctttt ttctcacgca
     1681 tcattttaag aaaatcggat gctgataggg gttcaagtcc cctgtgtttt ctctgttcgt
     1741 tcacagctgt cttaagaaag ttagtcatac ttaccccggg gatctcaaca gggtactgga
     1801 atccgaggaa tataccttca agggctcttt cgtcgggaga catttcgaga agatttctcc
     1861 caaggtatgt cactgtgcct tcagttactg tggcataatc tcttccggca ataacagcag
     1921 caaaagtact tttgcctgag ccgttgggcc ccataattgc atgtatctcc ccctccttta
     1981 cctcaaggga gattccctta agtatttccc tgcctccgac tgttgctctt aaatttttta
     2041 ttaccagcat aatatataat gaaaaaatta tcctatactg ccctcaagtg ttatctgcag
     2101 gagtttctga gcctcaactg caaactccat gggcaattta tttattatct ctcttgtgta
     2161 tccgtttaca atcaggctta cggcatcttc ggttgaaatg ccacgttgat tgcaataaaa
     2221 tatctgatcc tctccgacct ttgatgttgt tgcttcatgt tcaacaattg cagtcgggtt
     2281 ttcaacttcg atgtatggga atgtatgtgc cccgcattta tcaccaagaa gaagtgaatc
     2341 gcaccgggaa taattccttg cattgcgggc atttctgaga actttcacaa gacctctgta
     2401 actgttctgg ctacggcctg cagatattcc ttttgacact atggtgcttc tggtgttttt
     2461 cccgatatga atcattttgg taccggtatc agcctgctga aaatgattgg taactgctac
     2521 tgaataaaat tcacctgttg aattgtctcc tttcagcacg caggaaggat atttccatgt
     2581 aatagctgac cctgtttcca cctgggtcca tgatattttt gagttttccc cccgacacat
     2641 tcctcttttg gtaacaaaat tgaatatacc tcccctgccc tcatgatcgc caggatacca
     2701 gttctgaact gttgaatatt ttacttccgc atctttttcg gcaattattt cgaccactgc
     2761 ggcatgaagc tgattttcgt cgcgtatggg tgcagtacat ccttcaagat agctgacata
     2821 actttcatca tcagcaacaa taagagtgcg ttcaaactga ccggtttttg ctgcattgat
     2881 tctgaaatat gtcgagagct ccatcggaca tcttacgccc ttgggaatat agcagaagga
     2941 accatcactg aaaactgcgg aattcagagc agcaaaataa ttatcgctat atgggacaac
     3001 agtgccgaga tattttttta ccagatcggg atattccctt acagcttcac tgaaagaaca
     3061 aaaaataatt cccagttcgg caagtgtctc cctgaaggtt gtcttaaccg atacgctatc
     3121 catcacagca tcaacagcaa caccgctgag aattttctgt tcgtcgagag gtatgccgag
     3181 tctgtcaaaa gtttttttaa gctccgggtc aatttctgct gagcttttct tctttctgac
     3241 tgctggtgct gaataataga caatatcctg ataatttatt tccggaattt tcagatttgc
     3301 ccattcaggc atctccattg tgagccagtg cctgtacgat ttaagcctga attcaagaag
     3361 ccactcaggc tcattctttt tttccgaaat aagccttatg atgtcctcat tcagccctgg
     3421 aggtaccttc tccgcctcaa tatcagtata aaaaccatac ttatactcgc tgccggtaac
     3481 ctcatcaata atatttttct tcttttttcc ttcaggcata tgagcaaatg taattctgtt
     3541 attccggtat tataacaatt ttcgggtatt tttgttaaaa ttgaagccta tagaggcaca
     3601 aagttaataa agataggaga aaccattatc attgcctgaa taaataacct taaacttcaa
     3661 tcatttttta tccattccga aaatatttga actaaatttt aaataaaaac cagaaattat
     3721 tcaattattt atcaaatcat tttaaatttg gggctttaaa tttaatctac tttatatcaa
     3781 tgattaataa tagatttcca aacttttcag gcattttaaa gagattattt cttccactga
     3841 ttttgctcat cattccaata tccctctttg catcatcacc gggagaagtt ccttcgattg
     3901 gccctataag ggtggagttc atcatttttg gtatgatact tgccggtgtg gcactctttc
     3961 acaagcaaac attctgggtt gcagtgaccg gtctcacagt gttgctgatt tttaaattca
     4021 ttttt
//
LOCUS       DAOSNB010000242         4019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22522_length_4019_cov_4.667760, whole genome shotgun sequence.
ACCESSION   DAOSNB010000242 DAOSNB010000000
VERSION     DAOSNB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22522_length_4019_cov_4.667760"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLG42_09170"
     CDS             complement(<1..441)
                     /locus_tag="PLG42_09170"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOU31417.1"
                     /translation="MGKLLWKPGTMIYPLPAVMVSCGASEEEYNIITVSWTGTICTEP
                     PMCYISVRPERHSYGIIKKNMDFVINLTTEKLARATDWCGVNSGALFNKFKETGLTPV
                     RATIVKSPLIEESPVNIECVVREIKELGTHHMFISEVVAIHADEA"
     gene            695..3307
                     /gene="clpB"
                     /locus_tag="PLG42_09175"
     CDS             695..3307
                     /gene="clpB"
                     /locus_tag="PLG42_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOU31418.1"
                     /translation="MNLNNFTIKAQEAVMHAFDIAASMNHQAVECAHIMKGILTEADK
                     IVQFIFGKTGADIRAFSRALDRIIDSFPKVTGGESYLSSRAAEVLRKASGHALKMKDK
                     FVSVEHLLIALLESDDQVARLMKDSGIDMKGLMAAINDLRKGATVESQTADDTYNALN
                     RFAINLNEMAQSGKLDPVIGRDDEIRRVLQILSRRSKNNPLLIGEPGVGKTAIVEGLA
                     HRIVRGDVPEDLKSKVIYALDMGALIAGAKYKGEFEERLKSVINEVVSSNGEVVLFID
                     EIHTLVGAGKSEGAMDAANIMKPALARGELRAIGATTLNEYQKYFEKDKALERRFQVV
                     HVDEPDRADAISILRGLRERYETHHKVIIRDEAIIAAVDLSYRYITDRFLPDKAIDLI
                     DEAASRLRLQMNSLPEELDEIQRTIARLEKEREAVKRDQDKRKEEEISRELAKLMEKR
                     NELMAAWKSEKNLIEQMQEKKEAMEMIRLEAEQAERMGDYGRVAELRYGKLIELEKEI
                     AAIKEQIEERRRSGSLVREDVRAEDIAEIVSKWTGIPVSKMLESEREKLLRLEEELHK
                     RVVGQDEAIAAVADAVRRSRAGLQDEKRPVGSFIFLGTTGVGKTELARALAEFLFNNE
                     NLMTRIDMSEYQERHSVSRLIGAPPGYVGYEEGGQLTEAVRHKPYSVVLLDEIEKAHP
                     DVFNLLLQVLDEGRLTDNKGRTVNFRNVIIIMTSNLGSDIIRERMEQWNNNLPPREEE
                     KLRNEVFALLRKFMRPEFLNRVDEIVMFKPLTINQIKEIVRIQLGNLSKVLESQGIKL
                     EVGDDAVEWLAEHGFDPQLGARPVKRLIQREVVNRLSKEIIAGKVSKDDTVYLSVKDG
                     AIFFSNKKPSRRTT"
     gene            complement(3291..3626)
                     /locus_tag="PLG42_09180"
     CDS             complement(3291..3626)
                     /locus_tag="PLG42_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOU31419.1"
                     /translation="MVTSEKRGNIEIVSFTLNKLDALVADEVREEVSRLFNEPNSRVI
                     INLKGIQYIDSTGFGSLLSILRSARNNYGTLKICSIEPHVNNLFQNLRLNTIFDIYDD
                     VDECIKSFS"
     gene            complement(3639..>4019)
                     /locus_tag="PLG42_09185"
     CDS             complement(3639..>4019)
                     /locus_tag="PLG42_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOU31420.1"
                     /translation="TFFNLNYFFPMSYNYINPEYLLSVAGGDKETVKEIVEMFKAQVR
                     EISEEMKKLLKEADYYNLGLLAHKAKSSVAIMGMENLASMLKTFELQAKEGMETEKYS
                     TYISNYEHDTAEAIVELEKLINSI"
BASE COUNT         1261 a          735 c          923 g         1100 t
ORIGIN      
        1 agcttcatcg gcatgaatag caaccacttc tgaaataaac atatggtgcg tccccagctc
       61 ttttatttcc cttacaacgc attctatgtt tacaggagat tcttcaatca gaggggactt
      121 tactatcgtt gccctgaccg gtgtgagtcc tgtttctttg aacttgttga aaagtgcacc
      181 cgaattcaca ccgcaccagt cggttgcccg ggcaagtttt tcagttgtca ggttaataac
      241 aaaatccatg tttttcttta taataccata tgaatgccgt tcgggcctga ccgaaatata
      301 acacattgga ggttcggtgc aaatcgtgcc tgtccacgac actgttataa tattgtattc
      361 ctcttcactt gcaccacagc ttaccataac tgcaggaaga ggatatatca tggttccagg
      421 tttccataac agctttccca tacatttgaa tttataataa gacgggtaaa tttaattaat
      481 accttgctca caatgagcaa aattaatagt aaggtttcat tcacattcgt taattttcta
      541 cgaatcggtt tccatgtaat ccatatgtca taatgacatg ttaaaaatac cttaatatgt
      601 caaaaattac catgtaaatc aataagttgt atatggcagg gaatttgtaa aataaggtga
      661 gctaaaatat taaaaaagaa aggagtaaaa taatatgaac cttaataatt tcactataaa
      721 agcccaggaa gctgtaatgc acgcattcga catagccgca tcgatgaatc atcaggctgt
      781 ggaatgtgcg catattatga aaggtattct tacggaagcc gataagattg tgcagtttat
      841 tttcggcaag acaggtgcag atatccgtgc tttcagcaga gctctcgatc gcataattga
      901 ttcatttccg aaagttacag ggggtgaatc atatctttct tcgagagctg ctgaggtctt
      961 gcggaaggca tcgggtcatg cactgaagat gaaagacaaa tttgtatcgg ttgagcatct
     1021 gctgattgcg cttctcgaat cggacgacca ggtagccaga cttatgaagg acagcgggat
     1081 agatatgaag ggacttatgg ctgcaattaa cgatttgaga aaaggagcta ctgttgagag
     1141 tcagacggca gatgacactt ataatgcact taacaggttc gcaataaacc tcaatgagat
     1201 ggcacaatcg ggtaaactcg acccggttat cggaagagat gatgagatta gaagggtatt
     1261 acagatattg tcaaggcgtt caaaaaataa tcccttgctt attggtgagc ccggtgtcgg
     1321 aaagactgca atagtggaag gtctggcaca ccgtatcgtg agaggtgatg tgcctgaaga
     1381 ccttaaatcg aaggtaattt atgctctcga tatgggagca ctcattgcag gtgccaagta
     1441 taagggagag tttgaagaga ggcttaaatc ggtaattaat gaagtggttt cttctaatgg
     1501 tgaagtggtt ctttttattg atgaaattca tacacttgtt ggtgccggca aatcggaagg
     1561 agcaatggat gctgccaata ttatgaagcc ggctcttgca agaggagagt taagggctat
     1621 aggtgctaca actcttaatg aatatcagaa gtatttcgaa aaggacaaag ctcttgaacg
     1681 ccgttttcag gtagtacacg ttgatgaacc tgacagggct gacgccattt caattctcag
     1741 aggattgagg gaacggtatg aaactcatca taaagttatt attcgcgatg aagcaataat
     1801 agcagcagtg gatctgtcat atcgatatat taccgaccgt tttctgcctg ataaagccat
     1861 agaccttata gacgaagctg cttcgaggct gaggctgcaa atgaattcat tacctgaaga
     1921 gctcgatgag atacagagga caattgcacg ccttgaaaaa gaacgcgaag cagtgaaacg
     1981 agaccaggac aaaagaaaag aggaagaaat ttcgcgtgag cttgcaaaac tcatggaaaa
     2041 aagaaatgaa ctgatggcag cctggaagtc ggaaaagaat ctgatcgaac agatgcagga
     2101 gaaaaaggag gcaatggaga tgatcagact tgaagcagaa caggctgaaa ggatgggtga
     2161 ttacggccgt gtggcagagc tgaggtatgg gaaactgatc gaactggaga aagagattgc
     2221 tgcaataaaa gaacagattg aggaaagaag aagatctggt tcacttgtgc gtgaagatgt
     2281 acgagcagaa gatattgcag agatagtctc taagtggact ggaatacctg tttcaaaaat
     2341 gctcgagagt gagagggaaa aacttcttcg ccttgaggag gagcttcaca aaagggttgt
     2401 tggccaggat gaggcaatag ctgctgttgc cgatgctgta agaagatcga gggcaggact
     2461 gcaggatgaa aaaagacctg tgggctcatt cattttcctt ggaacaacag gtgtgggcaa
     2521 aaccgaactt gcccgagcac tggccgaatt tctgttcaac aatgagaacc tgatgacgag
     2581 gattgatatg tcagaatatc aggagagaca ttcggtatcg agacttatag gagctccccc
     2641 gggttatgtc ggatatgaag aaggcggaca gctgactgaa gctgtcaggc ataaacctta
     2701 ttcggtcgtc ctgctcgacg aaatagaaaa ggcacatccg gatgtgttta accttctgct
     2761 acaggtgctc gacgagggaa gacttactga taacaaggga agaacagtta acttcagaaa
     2821 tgtaataatt ataatgacct ccaacctcgg ttccgatatt atccgcgaga ggatggaaca
     2881 gtggaacaat aaccttccgc ccagggaaga agagaaacta aggaacgaag tctttgcatt
     2941 gctgagaaaa tttatgcgac ctgaattcct taacagggtt gatgaaatag taatgtttaa
     3001 accattgact attaatcaaa taaaagaaat agtaagaatt cagcttggca acctttctaa
     3061 agtacttgaa tcacagggca ttaagctcga agtgggtgat gatgcagttg aatggcttgc
     3121 tgaacatggg tttgatccgc agctgggtgc ccgaccggtt aaacgactga ttcagagaga
     3181 ggttgtgaat cgattgtcga aagaaataat agcaggaaaa gttagtaagg atgatactgt
     3241 ttatctaagc gtgaaagacg gagcaatctt tttcagcaat aagaaaccat ctaggagaac
     3301 gacttgatgc attcatcaac gtcatcgtaa atatcaaaaa tagtattcag acgcaggttc
     3361 tgaaaaagat tgtttacgtg aggctcaata gaacaaattt ttagtgttcc gtaattattc
     3421 ctggctgatc taagtattga aagcagggaa ccaaagccgg tactatctat atattgtata
     3481 ccctttaggt ttattatcac tctggagttt ggttcgttga ataaccttga tacttcttct
     3541 cttacctcat cagcaaccag tgcatcaagt ttatttagag tgaatgatac aatttcgata
     3601 ttacccctct tttcactggt aaccatggtt ccctgctttc agatactgtt tattaatttt
     3661 tcaagttcaa ctattgcttc ggcagtatcg tgctcataat ttgatatgta ggtgctgtac
     3721 ttttctgttt ccataccctc ttttgcctgc aattcgaaag tcttaagcat actggcaaga
     3781 ttttccatcc ccataattgc taccgacgat tttgctttat gtgccagaag tccaagatta
     3841 taataatcag cttctttaag taatttcttc atttcttctg aaatctcacg aacctgcgcc
     3901 ttaaacatct ccactatttc cttaacagtt tccttatcac cacctgccac tgacaacaga
     3961 tattcagggt taatataatt ataactcatt gggaagaaat aatttaaatt gaaaaaggt
//
LOCUS       DAOSNB010000243         4007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22610_length_4007_cov_4.906883, whole genome shotgun sequence.
ACCESSION   DAOSNB010000243 DAOSNB010000000
VERSION     DAOSNB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22610_length_4007_cov_4.906883"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PLG42_09190"
     CDS             complement(<1..1235)
                     /locus_tag="PLG42_09190"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOU31421.1"
                     /translation="MKRILSALLILALAIPLFSQKKEIARLTVEAGKFQRINTPVCYD
                     LSVLNIADTLSFQLYEDVKGKLIEVPSQVEEGYIPRLWWIMSGITPAGSKREYILYQD
                     IKTKTRPAVNVRLTPDVIRLIKEEKPVLDYAVKIIYPPAGVDTMYKRNGFIHPVYSPS
                     GNVLTRINAPDHYHHFGIWNPWTRVRIRNHVTDFWNLYTHQGTVRFSGVNSLTEGPVF
                     GGFNVRHEHIDFQGKKPEETTFNEVWDVRVWNTEPLSGSKAWLVDFTSYLSVACDTAV
                     VLEAYRYGGGIGFRATAEWNNKNSWVVTSEGKTRIDADGTRARWTDVGGMFEGKGESG
                     IVFLSHPSNREHPEPMRVWPVDQNGRGDVYFEFCPIRHKEWVLEPGNVYRLKYRMLIY
                     DGKVDRATSDRLWNDFAYPP"
     gene            complement(1245..2282)
                     /locus_tag="PLG42_09195"
     CDS             complement(1245..2282)
                     /locus_tag="PLG42_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOU31422.1"
                     /translation="MTDKQWNDLITLIEGKNPGYQPVGFIIDSPWIPGWYGINNIQYY
                     QSDRHWLASNLKAVNLFPDVWFLPGFWSEYGMCTEPSAFGARLKWMEQSLPHAEKVIK
                     NIEDIKDLPVPDVETDGLLPFMINRLRENEEEIKKAGHEIRFAVSRGPLNIASFLMGT
                     TEFMMALAMYPVESEQLIGHITEFICNWLRYQKKCFPSIGGIFILDDLMGFIGEYEFN
                     EYAIPAFRKIFNAFDCRVRFLHNDAQGLITAKHLKDINVNMYNFSFEHSMGEIRELAG
                     EEVVLVGNIPPRDVMAAGTPAQVKEAVKKAANEIKRHNRIIWSVGGGMPQGVKNENIE
                     AFIEGVREYIK"
     gene            complement(2279..>4007)
                     /gene="gcvPB"
                     /locus_tag="PLG42_09200"
     CDS             complement(2279..>4007)
                     /gene="gcvPB"
                     /locus_tag="PLG42_09200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOU31423.1"
                     /translation="ILPSGITTDDIKKRALQAGINFWYPSANIVSISTDEITTLEELR
                     TIAEIFAGAAGKKTPEINELCNCRIIENKFLRKSEFLKESVFNMYHSETAMMRYIKML
                     ERKDFSLTHCMISLGSCTMKLNPATAMFPMSWPEFANIHPFVPDDQAAGYHKLMEELG
                     NALKEITGMKEISFMPNSGAAGEYTGMLVIRQYHISRGEGHRKVALIPASAHGTNPAS
                     AAMAGMKVVVVECDEKGNISVEDLRKKAEENRDNLSCIMITYPSTHGVFEEDVIEIVD
                     IVHRNGGLVYMDGANLNAQIGFTSPGQIGADVCHLNLHKTFAIPHGGGGPGMGPILVN
                     EKLAPFLPAHPIVKTGGKQGITAVSSAPFGSAHILTISHAYIMMMGAEGLTEATRYAI
                     LNANYIASALKDCYKILYTGKNGMVAHEMILDCRRFKAEAGVSEVDIAKRLMDYGFHA
                     PTLSFPVHGTLMVEPTESEPLQELDKFITAMKSIWAEIQEIIEGKADREDNVLHHAPH
                     TEKVATSDNWNHKYTRQKAAFPAKWLSENKFWPSVGRIDDAYGDRNLVCTCEPIDAYR
                     QETFKLSES"
BASE COUNT          933 a          931 c          902 g         1241 t
ORIGIN      
        1 ggtggataag cgaagtcgtt ccataatctg tcggaggttg ccctgtcaac ttttccgtcg
       61 tagatgagca tacggtattt gagccgataa acattgccgg gttcgagaac ccactcttta
      121 tggcggatag ggcagaactc gaaatagaca tcaccccgtc cgttctggtc gacaggccat
      181 actctcatcg gttcggggtg ttccctgttg gaggggtggg agaggaagac tattcccgac
      241 tcgcctttgc cttcgaacat accgcctaca tcggtccatc tcgcccttgt gccgtcggca
      301 tctatccgtg tctttccctc agatgtaaca acccagctgt ttttattgtt ccattctgcc
      361 gtagccctga agcctatgcc tccaccatac ctgtaagctt caagtactac tgccgtatcg
      421 caggcaacag aaagataaga agtaaaatca acaagccagg cttttgatcc ggaaagcggc
      481 tctgtgttcc atacccttac atcccaaact tcgttgaaag ttgtttcctc gggtttttta
      541 ccttgaaaat caatgtgttc atgacgaaca ttgaatcctc cgaaaacagg tccttcggtc
      601 aacgaattga ctccggaaaa cctgactgtg ccctgatgag tatatagatt ccagaaatcc
      661 gttacatgat ttctgattct cacacgggtc cagggattcc atataccaaa atgatgataa
      721 tgatcgggtg cattgattct tgtaagtaca tttcccgaag gagaatagac aggatggatg
      781 aatccgttac gcttgtacat agtatccaca cctgctggcg gatatataat cttcaccgca
      841 tagtcaagga caggtttttc ttcttttatc aggcgaatga catcaggtgt tagtctgaca
      901 ttaacggcag gcctggtttt tgttttaatg tcctgataaa gaatatattc cctttttgac
      961 cctgccggtg taatgccgct cataatccac catagtcttg gtatatagcc ttcctcaacc
     1021 tgtgaaggaa cctcaatgag tttgcctttc acatcctcgt aaagctgaaa ggagagagtg
     1081 tcggcaatgt tcaacactga aaggtcgtag catacaggag tgtttattct ctggaatttt
     1141 cctgcttcaa ccgtaagcct tgcaatctcc tttttctgcg aaaagagagg gattgcaagt
     1201 gccagtatca acaatgcgga taaaatcctt ttcatggttt gaatttattt gatgtattct
     1261 ctgactcctt ctatgaaagc ttcaatattt tcattcttta ctccctgtgg cattcctccg
     1321 cccaccgacc atattatgcg gttatgtctc tttatttcat tggcagcttt tttcacagcc
     1381 tctttaacct gagccggtgt ccctgcagcc atgacatcgc gcggcggaat atttcctaca
     1441 agcaccactt cttcgccggc cagttccctt atttctccca ttgaatgttc gaaggagaaa
     1501 ttgtacatgt taacatttat atctttcagg tgttttgctg taatcaatcc ctgggcgtcg
     1561 ttatggagaa acctcaccct gcagtcgaag gcattgaata ttttcctgaa ggcagggata
     1621 gcatattcgt tgaattcata ctcacctata aagcccatca ggtcgtcgag tataaaaatt
     1681 cccccgatgg atgggaagca ttttttctgg tatctgagcc agttgcatat aaactctgta
     1741 atgtgcccga ttagttgttc tgattcgaca gggtacatgg cgagggccat catgaattcg
     1801 gttgtcccca tcaggaagct tgcaatgttt aatggtccgc gggaaacagc aaaccttatt
     1861 tcgtgacctg ccttttttat ttcctcctcg ttttccctta gtctgtttat catgaaaggc
     1921 agcagaccgt cggtttcgac gtcgggcact ggcaagtcct tgatgtcttc aatgtttttt
     1981 atgacttttt ctgcatgggg caggctctgt tccatccatt taagtcgtgc gccaaaggct
     2041 gatggctcgg tgcacatgcc atattccgac cagaaaccgg gaaggaacca gacgtcgggg
     2101 aacaggttta cggctttcag gttcgaagcc agccagtgtc tgtcggattg gtaatattga
     2161 atattgttga taccatacca ccccgggatc caggggctgt ctattataaa ccccactggc
     2221 tggtagcctg ggtttttacc ttcaatgagg gtaataagat cgttccattg tttatcggtc
     2281 atgactctga tagtttgaat gtttcctgcc ggtaagcatc aataggttcg caggtgcata
     2341 ccaggtttct gtcgccgtat gcatcatcta ttcttcccac tgaaggccag aatttgtttt
     2401 cggatagcca tttagcggga aaagcagctt tctgtctggt atatttatgg ttccagttat
     2461 ctgaagttgc cactttttct gtgtgtgggg cgtggtgaag aacattgtct tctctgtcgg
     2521 ctttgccttc gattatctcc tgtatctcag cccatattga tttcattgca gtgatgaatt
     2581 tgtcgagttc ctgaagcggt tccgattctg ttggttcaac catcagtgta ccgtgtaccg
     2641 ggaatgatag ggtaggggcg tggaagccat agtccataag gcgtttggcg atatccactt
     2701 cggacacacc ggcttcagct ttaaacctgc ggcagtcgag aatcatttca tgggcaacca
     2761 ttccattctt tcctgtatag aggattttat agcaatcttt gagagccgaa gctatatagt
     2821 tggcattcag tatggcatat ctggttgcct cggtgagtcc ttcagctccc atcatcatta
     2881 tgtatgcatg tgatattgtg agtatgtggg cactgccgaa tggagctgat gatacagcag
     2941 ttataccctg tttgccgcct gtttttacaa tggggtgggc tggcaggaac ggagccagtt
     3001 tttcgttcac aagtatcggt cccatgccag gtccgccgcc tccatgaggt atggcaaaag
     3061 ttttgtgaag gttaagatga caaacgtcgg cacctatctg gcccggactg gtaaaaccta
     3121 tctgagcgtt cagatttgca ccatccatgt aaacaagtcc gccgttgcgg tgtacaatgt
     3181 caacaatttc aatcacatct tcctcgaaga ctccgtgtgt tgagggatag gtaatcataa
     3241 tgcagctcag gttatccctg ttctcttctg cctttttgcg gaggtcttca acgctgatat
     3301 tccctttttc atcacactcc accacaacaa ctttcattcc tgccattgct gcacttgccg
     3361 ggtttgtacc gtgggctgat gcggggataa gtgcaacttt acggtgtcct tcacctctgc
     3421 tgatatggta ctggcgtatt accagcatac cggtatattc acctgcagct cctgaattag
     3481 gcataaatga aatctccttc ataccggtta tctctttcag ggcgttgcca agctcttcca
     3541 tcaatttgtg gtagccagca gcctggtcgt ctggaacaaa agggtgaata tttgcaaatt
     3601 caggccagct catcggaaac attgctgttg ccgggttgag tttcatggta catgagccca
     3661 gcgatatcat gcaatgtgtc aatgaaaaat ccttgcgctc cagcattttt atataccgca
     3721 tcattgcagt ttcgctatga tacatgttga atacagattc tttcaggaat tctgattttc
     3781 gcaggaattt attctcaatg atacggcagt tgcatagctc atttatttca ggggttttct
     3841 ttcctgcagc tccggcaaaa atttctgcaa tggttcttag ttcttctaat gttgttattt
     3901 cgtcggtgct tattgacaca atatttgcgg aggggtacca aaaatttatt ccggcctgga
     3961 gtgctctttt ctttatgtcg tctgtagtta ttcctgaagg cagaata
//
LOCUS       DAOSNB010000244         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22691_length_3996_cov_4.484902, whole genome shotgun sequence.
ACCESSION   DAOSNB010000244 DAOSNB010000000
VERSION     DAOSNB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22691_length_3996_cov_4.484902"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PLG42_09205"
     CDS             <1..1544
                     /locus_tag="PLG42_09205"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOU31424.1"
                     /translation="LLTFAGKPDGKTDVLRYEFIIRINDSTDIIFVEALSEVRFNTTS
                     GYVEFDFRNSDSPGKGMKVQNVNIGGNSAKWIHAGNKLKIFAEKSFVAGSVAELKVVY
                     SGIPADGLIISKNKFGARTFFSDHWPDRASYYLPVTDHPSDKAKVDFIIIAPFHYRVV
                     ASGRLIEESNVDSYNRLTHWHEEVPLPVKVMAFGAAPFATCLAGFAGNIPVWSWVFPE
                     NRNEGFNDYSIAVKAVEFYQKLIGPYPYEKLANVQSKTIFGGLENASCIFYSENSVTG
                     KGRAEGLIAHETAHQWFGNSVTEKEWSHIWLSEGFATYLTAVYMEMNYGRERLETEMR
                     QARERVLRYYDKNPAPVIDTSVINLMSLLNANSYQKGSWVLHMLRREVGEENFWKGMR
                     LYFERFRDSIAETNDFMNVMQEVSGRNLKQFFHQWLEVPGQPEIKISKQSSSRGVTDI
                     LIEQKQAYPFSFSIDLLVKTGGQEKIETVRVSERKTVISIKAGDIAEIVPDPHTWLLF
                     REVRN"
     gene            complement(1541..2251)
                     /locus_tag="PLG42_09210"
     CDS             complement(1541..2251)
                     /locus_tag="PLG42_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOU31425.1"
                     /translation="MENSITILAVTAVSLGFFHTLMGPDHYLPFIVLSQAKKWSTKKT
                     MLITFLCGLGHVLSSVVLGIIGIGIGISVNKLVSVESVRGNIAGWLFIAFGLVYTIIS
                     IRNLVRKKTHTHRHFHAGGLEHEHEHNHMKEHVHVHEEEIVSATPWILFLIFVFGPCE
                     PLIPLLMYPAAENNITGAIIVAILFSVVTIATMMSIVLVFKLGLTSINLKPLEKYNHV
                     IAGAVILLSGVAIQFLGL"
     gene            complement(2309..3121)
                     /locus_tag="PLG42_09215"
     CDS             complement(2309..3121)
                     /locus_tag="PLG42_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOU31426.1"
                     /translation="MNFRSKIKALYRRIISGMSANNNPLFIFFYKYFYRPPEGTIEEF
                     TDIFSRYRRKIKVVQVGANDGITNDPIHKFIKRDGWEGVLLEPQKYVFEKFLQPLYRK
                     TQGIITVNAALDRTDGYKPVYKISASNSRWATGLTSFNREVLEKALSSGYIEKQAKKE
                     GLKIEDSAASLITEEKVECISPETLITRYNLDRIDWLQIDTEGYDYEIIKMFNIEKTK
                     PCVIVYENLHLPEEQKRECREMLEKNGYRCRDFGPNSLAVLDPPECLQKFFA"
     gene            3226..>3996
                     /locus_tag="PLG42_09220"
     CDS             3226..>3996
                     /locus_tag="PLG42_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOU31427.1"
                     /translation="MKDSEQQDQSIKKLSLAGLILTLGIVYGDIGTSPLYTFKAIILG
                     GRDNFNDLLVYGGLSAIFWTLTLQTTIKYIIITLRADNNGEGGIFALYALIKKRSVAA
                     AILTMIGGSALIADGVLTPSITVTASVEGLKLIKPGFPVIPVVLIIFSALFFLQQFGT
                     AYLARWFGPVMVIWFSLLALLGFSQLIHTPEILKAVNPEYAYRFITEYPGGFVLMGAV
                     FLCTTGAEALYSDMGLCGRKNIRVSWIFVKTALLLNYFG"
BASE COUNT         1124 a          807 c          874 g         1191 t
ORIGIN      
        1 atttgctgac tttcgcggga aagcctgatg gtaagacaga tgttctgaga tatgaattta
       61 ttatacggat aaacgattcc accgatataa tatttgttga agctttatcc gaagtcagat
      121 ttaatactac ttccggttat gttgaatttg atttcagaaa ttctgactcc ccggggaaag
      181 ggatgaaagt tcagaatgta aatattggag ggaattcggc aaagtggata cacgccggta
      241 acaagcttaa gatttttgct gaaaaatcgt ttgtagcagg ttcggtggca gaactgaagg
      301 ttgtttattc gggcatacct gccgacggac tcataatttc aaagaacaaa tttggtgccc
      361 gcactttttt ttctgaccac tggcccgaca gggcttcata ttatttacct gttacagatc
      421 atccttccga caaggctaaa gtggatttta taataattgc tccttttcat tacagggttg
      481 tagcaagcgg cagactgatt gaggaaagca atgttgacag ttacaacagg cttacccact
      541 ggcacgaaga agttcctttg ccggttaagg ttatggcatt tggtgctgcg ccttttgcaa
      601 cgtgcctggc aggttttgca ggtaacatcc cggtatggag ctgggtcttt cccgaaaacc
      661 gtaatgaggg cttcaacgat tattctattg ccgttaaggc cgtggaattc tatcagaagc
      721 ttatagggcc ttatccttat gaaaagcttg ccaatgtgca gtcgaagacc atttttggag
      781 gacttgaaaa tgcctcatgc attttttaca gtgaaaattc tgtaaccggt aaggggagag
      841 ccgaagggct gatagcgcac gaaacggcac accagtggtt cggaaattcc gttactgaaa
      901 aagagtggag ccatatatgg ctcagtgagg ggtttgcaac ctatctcact gccgtatata
      961 tggaaatgaa ctatggcagg gagcggcttg aaacggaaat gcgccaggca agggagagag
     1021 ttttgaggta ttacgataaa aatcccgctc ccgtcatcga tacttcagtc attaatctga
     1081 tgagcctgct taatgcaaat tcgtaccaga aagggtcatg ggttcttcac atgctgaggc
     1141 gcgaggttgg tgaagaaaat ttctggaaag gcatgagact ttactttgaa agattcagag
     1201 acagcattgc tgaaacaaac gatttcatga atgtgatgca ggaggtttca ggcaggaatc
     1261 ttaagcagtt ttttcaccag tggctcgaag tgccgggtca gcctgaaatt aagatatcga
     1321 aacaaagctc atcgagaggc gttactgata tcttaataga gcagaagcag gcttatcctt
     1381 ttagcttttc aatcgacctg ctggttaaaa ctggcgggca ggaaaagatt gaaactgtaa
     1441 gagtcagcga aaggaaaaca gtaataagca taaaggcagg agacattgca gaaattgttc
     1501 ctgaccctca cacgtggctg ttgttcagag aagtaagaaa ttagagcccc agaaattgta
     1561 ttgctactcc agaaagaaga attactgcac ctgcaataac atgattatat ttctcgagcg
     1621 gcttcaggtt aatactggta aggccaagtt tgaaaaccag tactattgac atcattgttg
     1681 cgattgtcac tactgagaac aggattgcaa caataattgc accggtaatg ttattttcgg
     1741 ctgcagggta catcagaaga ggtataaggg gctcacatgg cccgaagaca aaaatcagaa
     1801 aaagaatcca tggtgtggct gagacaatct cttcttcgtg cacatgtaca tgctccttca
     1861 tgtggttatg ctcgtgctca tgctccagcc caccggcatg aaaatgcctg tgagtatgtg
     1921 tcttctttct caccagattc ctgatactga ttatagtgta aacaagtccg aatgcaatga
     1981 aaagccaccc tgcaatatta cccctcaccg actcgacaga tacaagcttg ttgaccgaga
     2041 tgccgattcc tatcccgata attcccagaa caacagaact aagtacatgc ccgagcccgc
     2101 agagaaaagt aatcaacatt gtctttttcg tactccattt ctttgcctgg ctcagaacaa
     2161 taaacggcag gtaatggtca ggccccatca gagtatgaaa aaacccgagc gatactgcag
     2221 ttacggccag aattgttatt gaattttcca ttaaaaagtt ttatgatttt gcaaattaaa
     2281 tcaaaaaatc agatttacct gacatatatt atgcgaaaaa cttctgaagg cattcgggag
     2341 ggtcgagaac ggcaagtgag ttggggccga aatccctgca tctgtagccg tttttttcaa
     2401 gcatctccct gcactccctt ttctgttctt caggcagatg aagattttca taaactatca
     2461 cacatggttt tgttttctca atgttgaaca ttttaattat ttcataatca tacccttcgg
     2521 tatcaatctg caaccagtca attctgtcga ggttgtatcg ggttatcagg gtttcgggcg
     2581 aaatgcattc caccttctct tcagttatta aactggcagc agagtcttca atcttcaatc
     2641 cctccttttt agcctgtttt tcaatatacc ctgaactgag agctttctca agcacttccc
     2701 tgttgaaact tgttagacct gttgcccatc ttgaattgct tgcagaaatt ttgtatacag
     2761 gcttgtaacc gtcggttcta tcaagagccg cattaactgt tattattccc tgggtttttc
     2821 tgtacagagg ctgaagaaat ttttcaaaaa catatttctg aggttccaga agaacacctt
     2881 cccagccatc tctctttata aatttatgta tgggatcatt cgttattccg tcgttggcgc
     2941 ctacctgtac aactttgatt ttccttctat accgtgaaaa tatatcggta aattcttcta
     3001 ttgtgccttc tggtggacga taaaaatatt tgtagaaaaa tataaagaga ggattattat
     3061 tggcactcat accggaaatt attctccggt aaagtgcttt gatttttgac ctgaaattca
     3121 tgaatatatt gttttattat ggggtgtaaa tttacattta atgtttttaa ttattaaatt
     3181 tgcgtccctt gaaaatcaga tatccggcta tgccggaaga aagttatgaa agattcagaa
     3241 caacaggatc aaagtattaa gaaactttct cttgccggtc tgatactgac tctcggtatt
     3301 gtctacggag atattgggac cagtcccctc tatactttta aagcaattat tctgggaggc
     3361 agggacaact tcaacgatct tcttgtttac gggggacttt cagcaatttt ctggactctt
     3421 acccttcaaa ccacaatcaa atatattata atcactctca gggccgacaa taacggggaa
     3481 ggtggaatat ttgcacttta tgcccttatt aaaaagaggt cggttgcagc agcgattctt
     3541 acaatgatag ggggttcagc cctgattgcc gacggcgtcc tgaccccttc aatcaccgtt
     3601 accgcttcag ttgaaggctt aaaacttata aagcccggat tccccgtaat acctgtagtg
     3661 cttataattt ttagtgcttt gtttttcctg cagcagttcg gaacagctta tcttgcaaga
     3721 tggttcggtc cggttatggt tatctggttt tccctgctgg cacttcttgg attttctcaa
     3781 ttaattcaca cccctgagat tctgaaggca gttaaccctg agtatgctta caggtttatt
     3841 acggaatacc ccggaggctt cgtgctgatg ggagctgtct tcctctgtac aacgggtgca
     3901 gaagcattat attctgatat gggcctgtgt ggaaggaaaa acatcagggt atcatggatt
     3961 tttgtcaaaa cagcattgct tctaaattat ttcggt
//
LOCUS       DAOSNB010000245         3970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_22877_length_3970_cov_4.063602, whole genome shotgun sequence.
ACCESSION   DAOSNB010000245 DAOSNB010000000
VERSION     DAOSNB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22877_length_3970_cov_4.063602"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLG42_09225"
     CDS             <1..335
                     /locus_tag="PLG42_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HOU31428.1"
                     /translation="PPPEPLSYAYCSDTRFFKQLPSYVRNVSLIYHEATFDKEKEDLA
                     ELTGHSTTTDAATVALKAGAGKLLIGHFSSRYKDVDQLVEEARSIFPETYAAEDGKTY
                     LITHNKIS"
     gene            370..1899
                     /gene="guaA"
                     /locus_tag="PLG42_09230"
     CDS             370..1899
                     /gene="guaA"
                     /locus_tag="PLG42_09230"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOU31429.1"
                     /translation="MQEKIIILDFGSQYTQLIARRIRELNVYAEIFPYNRFPEPDRNV
                     KGVILSGSPFSVRDENAPFPNLEGIRGKLPLLGICYGAQYLAHCCGGVVLPSEKREYG
                     RAMFYVKEIEDPLFRGISYGTQVWMSHGDTITRLPADYIITGSTADIDIASFRVSGEP
                     TWGIQFHPEVYHTSEGKKILENFVKEICGCSGDWTPESFIESAIKEIRDKTKGEKIIL
                     GLSGGVDSTVAAVLLNRAAGSNLDCIFVNNGLLRKNEFENVLESYKKLGLRVRSVDAS
                     DIFLKELEGISEPEQKRKIIGRVFIEVFESEAAKIKNVKWLAQGTIYPDVIESASVNG
                     PSATIKSHHNVGGLPEKMKLKVIEPLRLLFKDEVRRVGKALGIPDEILNRHPFPGPGL
                     AIRIIGPVTKEKLRILREADEIFIEGLKKHGLYDKVWQAAVILLPVQSVGVMGDERTY
                     ENAVVLRAVDSTDGMTADFSRLQYDFLGNVASEIINKVKGINRVVYDISSKPPSTIEW
                     E"
     gene            1967..3571
                     /locus_tag="PLG42_09235"
     CDS             1967..3571
                     /locus_tag="PLG42_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOU31430.1"
                     /translation="MKKLIFSALVLVQLIMISLEGASGQVLSENTFKLGRTLSLIDAY
                     YVDTVNINTLTEKAIVAMLMSLDPHSSYISAKDVREMNEPLLGNFEGIGIQFNILHDS
                     IVVVEPIKNGPAEKVGIKAGDRIVKINDENVAGINISTEGVRSRLMGPKGTKVDVTVY
                     RKGQSQLFYFTITRDRIPINSLDAAYMLNPETAYFKFNKFAATTEKEFIEKLTDMPVK
                     SIKNVIIDLRGNPGGYMLAAVALANHFLDGEKLVVFMEGRKTPREEFKSNGKGLLTNC
                     NLVVLTDENSASASEIFAGAIQDWDRGVIMGRRTFGKGLVQNGFYLNDGSQIRLTIAR
                     YFTPTGRSIQSPYNEGYQKYIENFLRRFRNGETLSADSIKFIDSLKCSTLVTRRTVYG
                     GGGIMPDVFVSEDTTYYSEYYAGLIRRSVFNSFALEYSDKNREKIMKTYKDFEDFKKK
                     FTFSDEDIRWFIKMGEEAGVKYNESQFRISHDHILKVLKALVANNYWQTTEYFRIYNE
                     GDRVIEKALMLLANPAEYKKILGYQN"
BASE COUNT         1335 a          669 c          875 g         1091 t
ORIGIN      
        1 tcccacctcc tgaaccgctg tcgtatgcct actgcagcga tacccggttt ttcaaacagc
       61 ttccatcata tgtaagaaat gttagcctca tatatcacga agctacattc gataaggaaa
      121 aggaagatct tgcagagctg acaggccatt caacaactac cgatgctgca actgtagcac
      181 ttaaagcagg agcaggtaaa ctgctaatcg ggcacttttc atcaagatac aaagatgtag
      241 atcagctggt tgaagaagca cgttcaatct ttcctgaaac ttatgcggcc gaagacggta
      301 aaacctattt aataacacat aacaaaatat cctgaaagtc ggttatattt aaattaaatt
      361 ttatagaagg tgcaggagaa aattattata ctcgattttg gttcacaata cactcaacta
      421 atcgcaaggc gaataaggga acttaacgta tatgccgaaa tctttcctta taaccgattc
      481 cctgaacccg atagaaatgt aaaaggtgta atcctttccg gtagcccgtt ttcggtaagg
      541 gatgaaaatg cgcccttccc caaccttgaa ggaataagag gtaaactacc cctgcttggg
      601 atatgctatg gagctcaata tctggcccat tgctgtggtg gagtggttct gccttcagaa
      661 aaaagagaat atgggagagc aatgttttac gtgaaggaaa ttgaagatcc acttttcaga
      721 ggaataagct atggaacaca ggtgtggatg tcgcacggag atacaattac cagattacct
      781 gcagattata ttataactgg ttcaactgct gacatagata tagcctcgtt tagagtatca
      841 ggtgaaccaa catggggcat tcagtttcac cccgaagttt accatacttc cgaaggtaag
      901 aaaatccttg aaaattttgt aaaggaaata tgtggctgca gcggcgactg gacaccggaa
      961 tcgtttattg aatctgccat aaaggaaatc agggataaaa ccaaaggtga aaaaattatt
     1021 ctgggtcttt ccggaggggt tgactcaact gtggcagcag tattgcttaa ccgtgcagct
     1081 ggcagtaatc ttgactgcat ttttgttaat aacggacttc ttcggaaaaa tgagttcgaa
     1141 aatgtgcttg agtcatataa gaaacttggt ctgcgtgtca gaagtgtcga tgcatccgat
     1201 atcttcctga aggagcttga gggtattagc gaaccggaac aaaagcgaaa aataatcgga
     1261 agggtattca tcgaggtatt cgagtcggaa gccgcaaaga taaagaatgt taaatggctg
     1321 gctcagggta ccatctatcc tgatgtgata gaatcagctt cagtaaacgg cccttcagcg
     1381 acaataaaat cgcatcataa tgtgggagga ctgccagaaa aaatgaaact taaagtcatc
     1441 gaacccctgc gcttgctttt caaagacgaa gtaagaaggg tgggtaaagc tctcggtata
     1501 cctgatgaaa ttctgaacag acatcctttc ccgggacctg gcctggcaat cagaataatc
     1561 ggaccagtaa ctaaggaaaa gctgagaatt ctcagggaag ccgacgaaat ttttattgaa
     1621 ggacttaaaa aacatggttt gtatgataaa gtatggcagg ctgccgttat attattacct
     1681 gttcagtcgg tgggggttat gggagacgag agaacttatg aaaatgcggt ggtactgaga
     1741 gccgttgact caactgacgg aatgacagct gacttttcaa ggttgcaata tgatttcctt
     1801 ggcaatgtcg caagtgaaat tattaacaag gtgaagggaa taaacagggt agtttatgat
     1861 ataagctcaa aacctccttc aacaattgag tgggaataaa gagtattcaa taattttgtt
     1921 ctgcgtaagt attatgaatt ttttaattaa aactcaaaaa aataaaatga agaagcttat
     1981 tttcagtgcg ttggttttgg ttcagctgat aatgatatca ttggaaggtg ctagtggtca
     2041 ggtgctttca gagaatacat ttaaactggg aaggacacta tcgctgatag atgcatatta
     2101 tgttgataca gttaatatta atacacttac cgaaaaggct attgtagcaa tgcttatgtc
     2161 gcttgaccct cattcctcat acatttcagc aaaagatgtg agggaaatga atgagccatt
     2221 acttggaaat tttgaaggca tcggcattca atttaatata cttcatgatt ctatcgttgt
     2281 ggtggaacct ataaagaatg gcccagctga aaaagtagga atcaaagcag gtgaccggat
     2341 tgtgaaaata aatgacgaga acgtcgcagg aataaacata agcacagaag gtgttagaag
     2401 cagactgatg gggccgaaag gtacaaaagt ggatgttacg gtttaccgta aggggcagag
     2461 tcaattattt tatttcacaa ttacaagaga cagaataccc ataaacagcc ttgacgcagc
     2521 ttatatgctg aatcctgaga cggcatattt caagtttaac aagtttgcgg caacaactga
     2581 aaaggaattt attgaaaaac ttacagatat gccggttaaa tcaattaaaa atgtaattat
     2641 agacttgagg ggaaatcctg gtgggtatat gcttgcagct gttgcacttg cgaatcattt
     2701 tcttgatgga gaaaaacttg tggtctttat ggaaggcaga aaaacacctc gcgaagaatt
     2761 caaatcaaat ggcaaaggat tgcttaccaa ttgcaatctc gttgtactaa ctgatgaaaa
     2821 ttcagcctct gcaagtgaaa tttttgcagg tgcaattcag gattgggacc gtggtgttat
     2881 tatgggacgc aggacatttg gtaaaggact ggtgcagaat ggcttttatt taaacgacgg
     2941 gtctcagata agactcacca tagcccgata ttttacccca accggaagat ccatacagag
     3001 cccctacaat gagggctatc agaaatatat tgaaaacttt ttaagacgat tcagaaatgg
     3061 cgaaacactt agtgccgata gtataaaatt tatagattca cttaaatgta gcacactcgt
     3121 aacgcgccgc acagtttacg gcggtggcgg cataatgcca gatgtgttcg tttcggagga
     3181 tactacttat tattctgaat attatgccgg acttatcaga agaagtgtat tcaattcatt
     3241 tgcactggaa tattccgata aaaacaggga gaaaataatg aagacgtaca aggattttga
     3301 agactttaaa aagaagttta ctttcagtga tgaagacatt agatggttca ttaaaatggg
     3361 tgaagaagca ggagttaaat acaatgaaag ccagtttcgt atatcacacg accatattct
     3421 gaaagttctt aaagctctgg tagcaaacaa ttactggcag accactgaat atttcagaat
     3481 atataatgaa ggtgacaggg ttatagaaaa agctttaatg ctgcttgcaa accctgcaga
     3541 atataaaaag atacttggtt accagaattg aaattactca atataaatga ttttagcatt
     3601 aagtcattga ataacagata aaaagatagt taaaacagta tgacagcggt atctgaacaa
     3661 attactgtga ttgatttcag ggatatatta ttcaaatcag ggaaaaaagc catacgtcaa
     3721 agaaaatagg ccatttacag catgatttta acatattcgt caaataatta taaatttact
     3781 ggtgagaatt tgcagaattt tagttacttt gaatttttat tatatttgtt actgaaaaag
     3841 taagagatct ttttctaaag gaagattaga tgggctttga tgtagagagc tacctcacgg
     3901 agcagaaaaa cggcgaagtt atcctttatt ataagggtaa cattgattct gaagtcatta
     3961 atcatgtgct
//
LOCUS       DAOSNB010000246         3946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23048_length_3946_cov_4.925726, whole genome shotgun sequence.
ACCESSION   DAOSNB010000246 DAOSNB010000000
VERSION     DAOSNB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23048_length_3946_cov_4.925726"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /gene="rpsB"
                     /locus_tag="PLG42_09240"
     CDS             complement(<1..688)
                     /gene="rpsB"
                     /locus_tag="PLG42_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOU31431.1"
                     /translation="MPRVTFEELLEAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLE
                     KTIEKLEEAASAMKHIARSGKKILFVATKKQAKEIVAERAKKVNMPYVTERWPGGMLT
                     NFPTIRKAVKKMASIDKMLADGTFANLSKREKLQITRQRAKLEKLLGSIADMTRLPSA
                     IFVVDVCKENIAVREAKRLGIPVFAMVDTNSDPSDIDFPIPANDDASKSIAVIVDVLC
                     KAIEEGLNERK"
     gene            complement(806..1192)
                     /gene="rpsI"
                     /locus_tag="PLG42_09245"
     CDS             complement(806..1192)
                     /gene="rpsI"
                     /locus_tag="PLG42_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOU31432.1"
                     /translation="MEVINATGRRKSAVARVYLTPGKGNITVNDRDYKEYFGHEILSS
                     VVTQPLVLLNVAEKYDIKANLDGGGIKGQAEALRLGISRALVKVDPEFRSVLKDNGFL
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            complement(1198..1653)
                     /gene="rplM"
                     /locus_tag="PLG42_09250"
     CDS             complement(1198..1653)
                     /gene="rplM"
                     /locus_tag="PLG42_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOU31433.1"
                     /translation="MNTLSYRTESANKTTVQKEWVLVDAEGQVLGRLASVIASLLRGK
                     HKPYFTPHVDCGDNVVVINAEKVILTGEKWNEKEYVRHTGYPGGQRITKAEDMLRKNP
                     IYLIEHAVKGMLPKNRLGRALFKNMHVYAGSEHKHEAQKPKKIDITNIK"
     gene            1936..2481
                     /locus_tag="PLG42_09255"
     CDS             1936..2481
                     /locus_tag="PLG42_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOU31434.1"
                     /translation="MVSKKSDETYCGKRLMADFYEQDVLTVAPSLLGKILVVKQPSGD
                     VLRYKITETEAYRGHEDRACHASKGKTRRTEVMFRRGGYVYVYFVYGMYWMLNVVTGP
                     ENEPQAALIRGIEGYEGPGRLTRVLGIDGTFYGEDLTSSKRIWIEDNSYKASYITSPR
                     IGINYAGQPWVDMPWRFILTA"
     gene            complement(2488..3510)
                     /locus_tag="PLG42_09260"
     CDS             complement(2488..3510)
                     /locus_tag="PLG42_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31435.1"
                     /translation="MKSNGKVFLDLKNISFIKNNEYFNPIVEGYTLIGSFLRPVITYA
                     PSEKFEISAGMHQQIYSGEPFSIKPQLLLSTRWKVTRNSTLTLGAFDGGNKHRMFDPH
                     FDSERIYTSYTENGASFVTETGKLFSDLWLNWENFIFRGDTVREVFTAGESFSYKCDF
                     IDGFISLNIPVQLKFKHFGGQISNYNGHVLTFLNIAGGARLNFNIGAKAGVPGVEYLH
                     FVYRELTGKGDMGITEGNASWLRLHYSYRSLYIGSYFWFSHNFYAPDGNQIYGSVSDY
                     QPGYIISRRKIWTGAVYLNLFPHELFEFMLGFEGYYDFNLKRMDTAIALHINFDRRIT
                     LFNIKE"
     gene            3707..>3946
                     /locus_tag="PLG42_09265"
     CDS             3707..>3946
                     /locus_tag="PLG42_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOU31436.1"
                     /translation="MDSSYIIFRRWNSITGWLMFLISTTVYLITMEPTVSFWDCGEFI
                     LSAFRLQVGHPPGAPMFIMLGRIATLFAGGDTSKVA"
BASE COUNT         1097 a          786 c          759 g         1304 t
ORIGIN      
        1 gttttctttc attaaggcct tcctcaattg ccttacaaag aacatcgact atgactgcaa
       61 ttgattttga tgcatcgtca tttgcaggta tagggaaatc gatatctgaa gggtctgaat
      121 ttgtgtccac cattgcaaaa acagggattc caagcctctt agcttcacgt actgctatat
      181 tttctttgca gacatcaacc acaaatattg ctgatggcag ccttgtcata tcggcaatac
      241 ttccgagaag tttttccagc tttgcgcgtt ggcgtgtaat ctggagtttt tcacgtttgg
      301 agaggtttgc aaaggtcccg tcggctagca ttttatcgat cgatgccatc tttttgactg
      361 ctttcctgat agtagggaag ttggttagca tacctcccgg ccatctctcg gtaacgtaag
      421 gcatatttac ctttttggct ctttcagcaa cgatttcctt tgcctgtttt tttgtggcca
      481 cgaagagaat ctttttgcct gaacgggcta tatgcttcat tgccgaagca gcttcttcaa
      541 gtttttcaat ggttttttcc aggtcgataa tatggattcc attgcgctcc ataaatatat
      601 aaggagccat agctggattc cactttcttt tcaaatggcc gaagtgtaca ccggcctcca
      661 gtaattcttc aaaagtaact cttggcattt tatggttttt aagtttacat tctgttttat
      721 caaccgcaga gtagttccgc tttgcggaag tcaatgcggt ttagatacta aacttatatc
      781 ttaaataaat tcacagattt ctttcctaac gtttactgaa ctggaatctt ttccttgctt
      841 tgggtcttcc gggttttttt ctttcaactt cccttggatc gcgggtaaga aaaccattat
      901 ctttcagaac tgacctgaat tcaggatcaa ctttaacaag tgcccgggag atgccaagtc
      961 tgagagcttc agcctgacct tttattccgc ctccgtcgag attggcttta atgtcgtatt
     1021 tttctgcaac attcagaaga accagtggct gagtaaccac actggaaaga atttcgtgtc
     1081 cgaaatattc tttataatca cggtcattta ccgtaatatt ccctttaccc ggggttaaat
     1141 agacccttgc gacagcagat ttccttcttc ctgtagcgtt aataacttcc atgcgaatta
     1201 tttaatgttt gttatatcga tttttttggg tttctgtgcc tcatgcttgt gctcacttcc
     1261 agcgtataca tgcatatttt taaacagggc tcttcccagc ctgtttttcg gtagcatacc
     1321 cttaactgca tgctcaatga gataaatcgg atttttcctg agcatgtctt cggcttttgt
     1381 tattctctgt cctccggggt aacctgtatg ccttacatat tctttttcat tccatttttc
     1441 accggtgaga attaccttct cagcattgat gaccactaca ttgtcgccac agtcaacatg
     1501 gggtgtgaaa tatggtttgt gcttcccacg cagcagagaa gcaattaccg atgccagacg
     1561 acccagaacc tgtccttcag catcaacaag tacccattcc ttttggactg tagttttatt
     1621 ggccgattct gttctgtaac ttaaagtatt cactttatat tgtttttatt tgatttactt
     1681 atatttataa aatatttacc cgattaacgg gtctgcaaaa atacaacata ttttcaaaaa
     1741 aaagaactgt atgagtaatt ttatttaatt cttatttaag ccagcaatcc gccatatatc
     1801 tttaattggt taattaatag attattatct gatactttta acttctttta atcattgaat
     1861 cggcataata cataaatacg atttcgatat ttataaaaat taagatatta gcagagaact
     1921 tttcctgaat aggttatggt atcgaagaaa agtgatgaaa catattgcgg taaaagactt
     1981 atggctgatt tttatgagca ggatgtactc accgttgctc cttcacttct agggaaaata
     2041 cttgtcgtga agcagccatc aggggatgtc ttaaggtata agataacaga gactgaagcc
     2101 tacaggggac atgaagacag ggcctgtcat gcctcaaagg gtaaaactcg gcgcacagag
     2161 gttatgttca ggcgtggggg atatgtttat gtttactttg tttatggtat gtactggatg
     2221 ctcaatgttg taaccgggcc tgaaaatgaa ccacaggcag cgttgataag aggaattgaa
     2281 ggctatgaag gacctgggag actgacccgt gtactcggaa ttgatggaac tttctacgga
     2341 gaagacctta catcttcaaa gcggatatgg atagaagaca atagctataa agcttcctat
     2401 attacatcac cacggatcgg tatcaactat gcaggtcagc catgggttga tatgccctgg
     2461 agatttattc tcacagcctg aacttattta ttccttgata ttgaacagag ttattctcct
     2521 gtcgaagttt atatgaagtg caatggcggt gtccatacgt ttgagattga aatcataata
     2581 tccttcgaac cccagcataa attcaaaaag ctcatgtgga aacagattaa gataaacagc
     2641 tccggtccag atttttcttc ttgaaataat atatccaggc tggtaatcag aaacactacc
     2701 ataaatctga ttgccatcgg gagcataaaa attgtgagag aaccagaaat aagagcctat
     2761 ataaagtgac cggtaactgt aatgtaacct cagccatgaa gcatttcctt ccgttatgcc
     2821 catatcgccc tttcctgtaa gttccctgta aacgaaatgg agatattcaa ctccgggaac
     2881 ccctgctttt gcgccaatat tgaaatttaa cctggctcct cctgcgatat ttaaaaaagt
     2941 cagtacatgt ccattgtagt tacttatctg acctccgaaa tgtttgaatt ttaactgaac
     3001 ggggatattt aaacttataa aaccatcaat aaagtcacat ttgtaactga aagattctcc
     3061 cgcagtaaat acctcccgca ctgtatctcc cctgaaaata aaattctccc agtttagcca
     3121 taagtcgctg aagagtttac ctgtctcagt tacaaatgat gcaccatttt cggtatagga
     3181 agtgtaaatt cgttcagaat caaaatgcgg gtcgaacatt ctgtgtttgt tacctccatc
     3241 aaatgctccg agagtaagag tactgttccg ggttactttc catctggtgc tcagaagaag
     3301 ttgtggttta atggaaaaag gttcacccga ataaatttgt tggtgcattc ctgcacttat
     3361 ttcaaacttt tctgaagggg cataagtaat aacaggcctt aaaaaacttc ctatcagagt
     3421 atagccttct acaataggat tgaaatattc gttgttctta ataaaactta tgtttttaag
     3481 atccagaaaa actttaccgt tacttttcac taaagactct atctcctgag ctgttaattt
     3541 tgtcgcgcag cagatcaaaa acaaaaaaga aaaaatctgt ttcatcagtt ttatttgagc
     3601 cattaaaaat aataaattgt attgtttaca ggttaaaatg gcatcataat gaatcacaag
     3661 tgattttttt tgcgttattt gtctggcatt tgtaatcaat tcaatcatgg attcatcata
     3721 catcattttc aggcgatgga atagcattac cgggtggttg atgtttctga tttccactac
     3781 agtatatttg attaccatgg aacccactgt aagtttctgg gattgtggtg agtttattct
     3841 ttcagctttc aggcttcagg tagggcatcc tcccggtgct ccgatgttta ttatgcttgg
     3901 gcgtattgct acgttgtttg caggaggaga tacatcaaaa gtggca
//
LOCUS       DAOSNB010000247         3927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23185_length_3927_cov_3.260072, whole genome shotgun sequence.
ACCESSION   DAOSNB010000247 DAOSNB010000000
VERSION     DAOSNB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23185_length_3927_cov_3.260072"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /gene="clpX"
                     /locus_tag="PLG42_09270"
     CDS             <1..977
                     /gene="clpX"
                     /locus_tag="PLG42_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOU31437.1"
                     /translation="AVYNHYKRLMQKPEKDDVEIEKSNILLVGETGTGKTLLARTIAR
                     MLHVPFTIVDATVLTEAGYVGEDVESILTRLLQNADYDVKAAERGIVFIDEIDKIARK
                     GDNPSITRDVSGEGVQQGLLKLLEGSVVNVPPQGGRKHPEQKMIPVDTTNILFICGGA
                     FEGIEKKIAQRLNTTIVGYRATKDRERIDKDNLLQYIAPQDLRAYGLIPEIIGRLPVL
                     TYLHPLDRKALREILTEPKNAITKQYIKLFKMDNIDLTFTEEVLDYIVDKAIEFRLGA
                     RGLRAICEAIMLDAMFEMPASEDRELVITTDYAKRKLEKIDMKILKAS"
     gene            complement(1027..1890)
                     /locus_tag="PLG42_09275"
     CDS             complement(1027..1890)
                     /locus_tag="PLG42_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31438.1"
                     /translation="MKTKLSSIIVMLSFMFFASCQKDTAPLEESSINTADDDAVVNVL
                     FDDVFNTVDYASVLLDDALGKGETKSEAFVLADSCPVVTVDNLVPNVWPKTITIDYGA
                     GCTGFYGSTRSGKIIITITQRRNVTGAKRTVTFDNYYFNGIKVEGTKEFTNLGPNQNQ
                     NVVIEVKLTGGKLTLPDGKTIERSVNHQREWIAGWLTRNIWDDECLVTGTATGKTIDG
                     KSYSNTITTALHWKRVCEFFVSGVIKIEREGVEPFELDYGDGECDAYATVRRGEYSKQ
                     ITLRHRHRLMP"
     gene            complement(1981..2466)
                     /locus_tag="PLG42_09280"
     CDS             complement(1981..2466)
                     /locus_tag="PLG42_09280"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOU31439.1"
                     /translation="MISIIAAVSEDLGIGKDNKLLWNIPEDLKRFKKLTTGRCVIMGK
                     KTWESLPVKPLPGRKNIVVTDNPGDRFNCDCTAFSVEEALQACKGEEEVFVIGGGSIY
                     RQFMPLADRLYITHVHDKAPADTFFPEIDPSVWEIISEEFHDGIPGYCPSYTYRIYRK
                     K"
     gene            complement(2475..3269)
                     /locus_tag="PLG42_09285"
     CDS             complement(2475..3269)
                     /locus_tag="PLG42_09285"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOU31440.1"
                     /translation="MKQYLDLLDHVLKNGVRKNDRTGTGTISVFGYQMRFDLSKGFPL
                     LTTKKLHFKSIAYELLWFIRGDTNIKFLNDNGVRIWDEWADAEGNLGPIYGYQWRSWS
                     APDGRKIDQLMNVIENIKKNPDSRRHIVSAWNVGDLDRMALPPCHVLFQFYVAEGKLS
                     CQLYQRSCDIFLGVPFNIASYALLTMMVAHVTDLAPGEFIHTLGDAHIYLNHIEQVKL
                     QLTRQPYPLPEVRLNRDVRDIRDFRYEDIELVNYVAHPHIKGEISV"
     gene            complement(3266..>3927)
                     /locus_tag="PLG42_09290"
     CDS             complement(3266..>3927)
                     /locus_tag="PLG42_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HOU31441.1"
                     /translation="FHGAYKPDGLGRTYPNAITREGVKGMEHSKWSKDVSPEHDVTIT
                     FTRMVAGPMDYTPGAMINMERANFQPMFTRPSSQGTRVHQMAMYVVFESPLQMLSDSP
                     SNYEREKECTGFIAKVPVVWDDLKVLQAKVGDYILLARRSGNVWYVGGMTDWNARELE
                     LDLSFLEKGEYIMEVFQDGINADRHAQDYKHLTLEVTPGIKHKIYMAPGGGWVAKISP
                     K"
BASE COUNT         1109 a          932 c          772 g         1114 t
ORIGIN      
        1 tagctgttta caaccactat aaaaggctca tgcaaaagcc tgaaaaagac gatgttgaaa
       61 ttgaaaagtc aaacatcctc cttgtgggcg aaacaggtac gggaaagacc cttcttgcaa
      121 gaaccattgc ccgtatgctt catgtcccat ttacaatagt cgatgctact gtgcttaccg
      181 aagccggata tgtgggcgaa gatgtggaaa gcattctcac acgattgctc cagaatgccg
      241 actatgacgt aaaagccgct gaaagaggca tcgtttttat agacgagata gacaaaattg
      301 ccagaaaagg cgataatccc tccatcacga gggatgtctc aggcgaggga gtccagcagg
      361 gattgctaaa actgctcgaa ggctcggtgg taaatgtacc gcctcagggc ggaagaaaac
      421 accccgaaca aaagatgata ccagttgata ccacaaacat cctttttatc tgcggtggcg
      481 cattcgaagg tatagagaaa aaaattgcac agcgtctcaa cacaacaatt gtgggttacc
      541 gggcaacaaa agacagggaa agaattgaca aagacaacct gttgcaatat attgcacctc
      601 aggaccttag agcgtatggc cttatacccg agataatcgg ccgcctgcct gtgctcactt
      661 accttcaccc gctcgaccgc aaagccctgc gggaaattct tacagaacca aaaaatgcca
      721 tcacaaaaca atatataaaa ttgtttaaga tggataacat tgacctcaca ttcactgaag
      781 aggtactgga ttacatagtt gacaaagcta ttgaattccg gctcggggca agaggactga
      841 gggcaatctg cgaggctata atgctcgatg caatgttcga aatgcctgcc tcagaagaca
      901 gagagcttgt gataacaacc gattatgcaa aacgcaagct cgaaaaaatt gatatgaaaa
      961 tacttaaagc atcataaaga aaggggctga attcacagcc cctttctttt tcttgtcaaa
     1021 tgtattttaa ggcatcagcc tgtgtctgtg tcgtaatgtt atctgcttgc tgtactctcc
     1081 ccttctgact gttgcatatg catcacattc accgtcaccg taatccagct caaaaggttc
     1141 tacaccctcc ctctcaatct ttataacgcc acttacaaag aattcgcaaa ctctcttcca
     1201 gtgaagtgcc gttgtaatag tattcgaata tgacttcccg tcaattgtct tacctgttgc
     1261 agtaccggtc accagacact catcatccca aatatttctt gttaaccacc ccgcaatcca
     1321 ttccctctgg tgattaactg aacgctcaat cgtctttccg tcaggcaggg tcagcttacc
     1381 tcctgtaagc ttcacttcaa taaccacatt ctggttctga ttcgggccaa gattggtaaa
     1441 ctccttcgtt ccttcaacct ttataccatt aaaataatag ttgtcgaatg ttactgttct
     1501 ctttgcccct gtaacattcc tgcgctgtgt aatggtaatg ataattttcc ccgagcgtgt
     1561 gcttccgtaa aatcctgtgc aacctgcacc gtaatcaatt gtgatggttt ttggccaaac
     1621 atttggcaca agattatcaa ccgttactac cggacacgag tctgccagaa caaatgcctc
     1681 cgattttgtt tcaccttttc ccagggcatc atcaagtaga accgaagcat aatcaacagt
     1741 attgaacaca tcatcaaaca gcacatttac cacagcatcg tcatctgctg tattaatgct
     1801 cgactcttcg agcggagccg tatccttctg gcatgatgca aaaaacataa atgaaagcat
     1861 tacaataatt gaagaaagtt ttgttttcat agtaatctcg tttttgatta ataattctga
     1921 tttctcacct ttttagatag aatttattcc ggaaggttta acggagaaat ctgaaaaaag
     1981 ttactttttt ctgtaaattc ggtaagtata ggaaggacaa taaccaggta ttccatcgtg
     2041 aaactcttct gaaataatct cccacaccga tgggtctatt tcggggaaaa acgtatcagc
     2101 cggggcttta tcgtgcacat gggtaatata aagccggtcg gcaagcggca taaactgtct
     2161 gtatatgcta ccgcctccta taacaaacac ctcctcttca cctttgcatg cttgcaaagc
     2221 ctcttctact gaaaaagcag tacagtcgca gttgaatctg tcaccgggat tatcggtaac
     2281 tacaatattt tttctgccgg gaagcggttt cacaggaaga gattcccatg ttttcttccc
     2341 cataataaca cacctgcctg ttgtaagctt tttaaacctt ttgaggtctt caggtatgtt
     2401 ccagagtaat ttattatctt tcccgatacc gagatcttcc gaaacagcag ctattattga
     2461 aatcataaaa ataattaaac tgaaatctct ccttttatat gaggatgggc aacataatta
     2521 acaagttcaa tatcttcata tctgaaatcc cgtatatctc tcacatctct gttaagcctt
     2581 acctcaggga gaggataagg ctggcgtgtc agttgcagct tcacctgctc tatgtggttg
     2641 aggtaaatat gcgcatctcc aagagtatga atgaattcac ctggtgccag atcagtcaca
     2701 tgtgccacca tcattgtaag cagggcatag gatgcaatgt tgaaaggtac gcccagaaat
     2761 atgtcgcagc ttctctgata aagctggcat gaaagtttgc cttcagcaac ataaaactga
     2821 aataacacat ggcatggagg aagagccatc cggtcgaggt cgcctacgtt ccatgcactt
     2881 actatatgcc gtctcgaatc tggatttttc ttaatatttt caataacatt catcagttga
     2941 tcaatctttc tgccgtcggg ggcactccac gacctccact ggtatccata tatgggtccg
     3001 aggtttcctt cagcatcggc ccattcatcc catattctta caccattatc attcaggaat
     3061 ttaatattgg tatctcctct gatgaaccac aatagttcat acgcaataga tttgaaatga
     3121 agtttttttg ttgtaagcaa tgggaaacct ttgcttaaat caaatcgcat ctggtaaccg
     3181 aacacactta ttgtaccggt acctgtgcgg tcgttttttc tgacgccgtt tttcaggaca
     3241 tgatccagaa ggtcgaggta ttgtttcatt tcggagaaat ttttgcaacc catccaccac
     3301 caggagccat ataaatttta tgctttatac ccggtgtcac ttccagggta aggtgtttgt
     3361 aatcctgggc atgcctgtcg gcattaatac cgtcctgaaa tacttccatt atatattccc
     3421 ccttttcaag gaatgaaagg tcaagctcca gctcccgggc gttccagtcg gtcataccac
     3481 ctacatacca tacatttccg ctacgtctgg caaggagaat ataatctccc acctttgcct
     3541 gaagcacttt caggtcgtcc cacacaacag gtacttttgc aatgaaccct gtgcattcct
     3601 tttcacgttc atagtttgac gggctatcgg aaagcatctg cagtgggctt tcgaaaacca
     3661 catacatagc catctggtgc acacgtgttc cctgacttga tggccttgtg aacatgggct
     3721 ggaaatttgc cctttccata ttaatcattg cccccggagt ataatccatt ggtccggcta
     3781 ccatacgcgt gaaggtaatt gtaacatcgt gttcgggtga cacatctttg ctccatttgc
     3841 tgtgttccat acccttaacg ccttcgcgtg tgattgcgtt tggataagta cgtcctaaac
     3901 cgtcgggttt atatgcccca tgaaaat
//
LOCUS       DAOSNB010000248         3921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23225_length_3921_cov_4.121314, whole genome shotgun sequence.
ACCESSION   DAOSNB010000248 DAOSNB010000000
VERSION     DAOSNB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23225_length_3921_cov_4.121314"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLG42_09295"
     CDS             <1..822
                     /locus_tag="PLG42_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31442.1"
                     /translation="MYKVSSEKNYDLLILRNNGNQIFSASGETEWLEKKIEIQEGFNI
                     LEWIYKKDQSVYSGSDCAWIDFIRFPPDAFNKIDLKTGKIISPVADKKLDKEVVSAEI
                     INLGLDTIKQFNLAYTVNNKPPVTEIFKRTINPGDTVTVSFSTLVDMWQSGTYIINVY
                     SFNNNDSYLNNDTASVTIINTPVEKIFDPLNYARVMPNPFSDRFDISIGSKFYEYVNA
                     ELIDITGNTLWKGRFELIPGDNVITITPGDITSGYYTLRIKGKTISVAVRLIKTD"
     gene            874..1452
                     /locus_tag="PLG42_09300"
     CDS             874..1452
                     /locus_tag="PLG42_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOU31443.1"
                     /translation="MNIPDRKQALELFKKYNTSESLYKHALAVEGVMRYMARKAGEDE
                     EKWGVIGLVHDLDYEMYPDQHCKMTEKILRENGWPEEYIRAILSHAWGFCTDVEPVTR
                     LEKTLFAVDELTGLVVTSALVRPSRSVLDMEAKSVKKKWNDKRFAAGVNRQVIEKGAE
                     MLGVSLDELITDCIMGMREVAEEIGLKGNPVS"
     gene            complement(1531..3027)
                     /locus_tag="PLG42_09305"
     CDS             complement(1531..3027)
                     /locus_tag="PLG42_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31444.1"
                     /translation="MKETNKISRRSFIGTTGAVAAGFTILPSSVISGLGHRPPSDKLN
                     IACVGIGGMGNSNLRNVAPTENIVALCDVDWEYSKKVFETYPNAKRYWDWRKMFDEMG
                     KSIDAVIVATADHTHAIIAAHAITLGKHVYVQKPLTHTVYESRLLTKLAAKYKVATQM
                     GNQGASDEGVNLIIEWIQNGEIGEVTKVEAFTDRPIWPQGLNKPAKGEWVPDTLNWDL
                     FIGPAPMRPYHSIYHPWNWRGWWDFGTGALGDMACHILHPVFKALKLKYPTKVQASST
                     LLLSDCAPNAEMVRLIYPERPAPKGSKIKYPQVEVTWYDGGLQPMKPAGWPAGKNMND
                     GGGGVIFHGTKDKLICGCYGRNPWLLSGRVPNVPKTERRIEKATSGGHEMDWVRACKE
                     SPESRVPTKSDFAEAGPFNEMVVMGVLAVRLQGLNKELEWDGEKMEFTNISDNDTLRI
                     CLEDNFTVTDGHPTFNRKYTEPMSAKQFAAEMIKHTYRKGWSLPDMPA"
     gene            3180..3650
                     /locus_tag="PLG42_09310"
     CDS             3180..3650
                     /locus_tag="PLG42_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31445.1"
                     /translation="MEKKSFSLEMNESNLLTKVFKFILGVLCIAIAIYWIIFNLKMSA
                     IDWSSWVSSAFLGLFGLFMIWTSFGYGYNFIEFERDIIRLKNNSFLPVKEIKSTEIEK
                     IIIYPLKFIIRLKSAKSILTRFGISDIERNENIKDELMKYAESNKILLEIKNEM"
     gene            complement(3684..>3921)
                     /locus_tag="PLG42_09315"
     CDS             complement(3684..>3921)
                     /locus_tag="PLG42_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOU31446.1"
                     /translation="AEYGLSQVFIAVNPENLFNYPSMENSVRLIIEDFKKSIPEVNNQ
                     HVRYPGEKALKTRKENLINGIPVNKQIWDTILNL"
BASE COUNT         1174 a          732 c          819 g         1196 t
ORIGIN      
        1 atgtacaaag tgtcatcaga aaagaattac gacctgctaa tcttgaggaa taacgggaat
       61 cagatttttt ctgcttcggg agagacggaa tggctcgaga agaaaataga aattcaggaa
      121 ggattcaata tacttgagtg gatttacaaa aaagatcaat cggtttattc gggttcagac
      181 tgtgcatgga ttgattttat acgctttccg cccgatgcct ttaacaaaat tgatctgaaa
      241 acaggaaaaa ttatttcacc tgtcgcagac aaaaaacttg acaaagaggt tgtatcagct
      301 gaaataatca atcttggtct tgataccata aaacaattca acctggcata tacagttaat
      361 aacaagccgc cggttacaga aatatttaaa agaacaatta accccggtga tactgtaaca
      421 gtttcatttt caacacttgt tgatatgtgg cagagtggta catatataat caatgtttat
      481 tcattcaaca acaacgattc gtatctgaat aatgatacgg cttccgttac catcattaac
      541 actcctgttg aaaagatatt tgatccgcta aattatgcaa gggtaatgcc caatcctttc
      601 agcgaccggt ttgatatttc tatcggttca aaattttatg aatacgttaa tgcggaatta
      661 attgacataa caggcaatac attatggaaa ggaaggtttg aactaattcc gggcgataac
      721 gtaataacaa taactccagg agatataact tcaggttatt atacgttaag aataaaaggc
      781 aaaactattt ccgtggcagt cagattgatt aaaactgatt agtcatatta tttttaaccg
      841 gattttcaat attttcgtta aaaactgatt gatatgaata tacctgacag aaaacaagct
      901 cttgaacttt ttaaaaagta caacacatcg gagagtcttt acaagcatgc tcttgctgtt
      961 gaaggtgtga tgcgttatat ggccaggaaa gcaggcgaag atgaagaaaa atggggagta
     1021 attggtcttg tgcatgacct tgattatgag atgtatccgg atcagcattg caaaatgact
     1081 gaaaagattc taagggagaa tggttggccc gaagaatata taagggcaat attgagtcat
     1141 gcctggggtt tttgtactga tgttgaacct gttacacggt tggaaaaaac cctgtttgcc
     1201 gtagacgagt taacagggct agtagttacc agtgctctgg taaggccatc gagaagtgtt
     1261 ctcgacatgg aagcaaaatc tgtaaagaaa aaatggaatg ataaaaggtt tgcagcaggt
     1321 gtgaacaggc aggtgataga gaaaggtgcg gaaatgcttg gagtaagtct ggatgagtta
     1381 ataactgact gcattatggg tatgcgggag gttgctgaag agataggtct taagggaaat
     1441 ccggtgtcgt aatcctgata ttaagacaca caaaaataaa agcaggtgca tgaaatttca
     1501 gcacctgctt tatttttatg ccataataaa ttatgctggc atatcaggca atgaccatcc
     1561 tttgcggtag gtatgtttaa tcatttcggc tgcaaactgt tttgcagaca tcggttcggt
     1621 gtattttctg ttgaatgttg gatggccgtc tgttacagtg aagttatcct caaggcatat
     1681 tctgagggtg tcattgtcag agatgtttgt gaattccatc ttttcgccat cccattcaag
     1741 ttccttattg agaccctgca gtctaacagc cagtacaccc attaccacca tttcgttgaa
     1801 tgggcctgct tctgcaaagt cggacttggt aggaactctg ctttccgggc tttctttgca
     1861 ggctcttacc cagtccattt cgtggccacc gcttgtggcc ttttcaattc ttctctcagt
     1921 cttaggaaca ttaggtacgc gacccgagag cagccatgga tttctgccgt agcaaccgca
     1981 tatcaattta tcttttgtcc cgtggaagat aactcctcca cctccgtcgt tcatattctt
     2041 gccagctggc cagccggcag gtttcatcgg ctgaagacca ccatcgtacc atgtaacctc
     2101 tacctgagga tattttatct tggaaccttt tggagcgggt ctttcagggt agatcaggcg
     2161 taccatctct gcgttaggag cgcagtcgga aagtaacaat gttgagcttg cctgaacttt
     2221 agtggggtat ttgagtttca gtgctttgaa aaccgggtgc agtatatggc aagccatatc
     2281 gccgagggcg cctgttccaa aatcccacca gcctctccag ttccatgggt ggtagatgct
     2341 gtggtatggg cgcataggag ccggaccgat gaagagatcc cagttgagtg tatcgggcac
     2401 ccattcacct tttgcaggct tgttcagtcc ctgtggccag ataggacggt cggtgaaagc
     2461 ttcaaccttt gtaacttcgc ctatctcacc attctgaatc cattcaataa taaggtttac
     2521 accttcatct gaagctccct ggttgcccat ctgtgttgcc accttatatt tggcagccag
     2581 tttggtaagg agcctcgatt cgtaaactgt gtgcgtaagt ggtttctgaa cgtacacatg
     2641 cttacccagt gtaatagcgt gggcagcaat tattgcatgg gtatgatcgg ctgttgcaac
     2701 tataacggca tcgatcgatt tacccatttc atcgaacatc ttacgccaat cccagtacct
     2761 tttggcattg gggtaagttt caaaaacctt cttcgaatat tcccagtcaa catcgcacag
     2821 tgcaacgatg ttttctgtgg gggcaacatt cctcaggttt gaattaccca taccccctat
     2881 acccacacag gcaatgttaa gcttatcgct gggaggcctg tgtcccagtc cactaataac
     2941 tgaacttggc agaatagtaa acccggctgc tacagcaccg gttgttccaa taaaagacct
     3001 tcttgaaatc ttgtttgttt ctttcataac aggtttattt ttgggtttca gaattaaaac
     3061 ttatttcaac gataaaagta ataatttcac tataaataaa aaattttcac aataaagaat
     3121 atcataaagg atggttaatt ttgcataaaa tcttgttaac cggaagaatc agatctgcca
     3181 tggagaaaaa gtctttttcc ctcgaaatga atgaaagcaa cttacttaca aaagtcttta
     3241 aatttattct gggagttctt tgtatagcaa tagctattta ttggattata tttaatttaa
     3301 aaatgtcagc aattgactgg tcatcatggg tctcgtcagc gtttcttggt cttttcggat
     3361 tgttcatgat ctggacatca tttggttacg gatataactt tattgaattt gaaagggaca
     3421 tcataagatt aaaaaacaac tcatttcttc ccgttaagga aataaaaagc actgaaattg
     3481 agaaaatcat tatttatcct cttaaattca tcataaggct taagtctgct aaaagtatac
     3541 ttacacggtt cgggatttca gatattgaga gaaatgaaaa tatcaaagat gaattaatga
     3601 aatatgcgga gagcaacaaa atattgctag aaataaaaaa tgaaatgtaa atattaatgc
     3661 actaattcaa tttaatatca aaatcagaga tttaaaatag tgtcccatat ctgtttattt
     3721 acagggattc catttattag attttctttt ctggttttca gagctttttc tccagggtaa
     3781 cgaacatgtt gattatttac ttcaggtatg gattttttaa agtcttcaat tattaatctt
     3841 acagagtttt ccattgaagg ataattgaaa aggttctccg ggtttactgc aatgaacacc
     3901 tgtgatagtc cgtattcagc a
//
LOCUS       DAOSNB010000249         3920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23232_length_3920_cov_4.175938, whole genome shotgun sequence.
ACCESSION   DAOSNB010000249 DAOSNB010000000
VERSION     DAOSNB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23232_length_3920_cov_4.175938"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLG42_09320"
     CDS             <1..480
                     /locus_tag="PLG42_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOU31447.1"
                     /translation="IPGVSNPIKLGLAGGSLIVAMFLSKFGHRFSLIPYTTTSANLML
                     RETGIVLFLASVGLRAGEKFIPSLTSGDGFIWMGYGAIITVLPVIFTGILAIRWMKYN
                     YFEACGLLAGSMTDPPALAYANNMAQSEIPAVAYATVYPLVMFLRILVAQLLILLFV"
     gene            complement(542..1525)
                     /locus_tag="PLG42_09325"
     CDS             complement(542..1525)
                     /locus_tag="PLG42_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOU31448.1"
                     /translation="MYFIKIRSCPIIKSIIFASLFEKINYVIFPQPSWRICSASDSFF
                     RTITALANEKASKKIGSLSLNIIRMFLAIFLLSFYNLIARGHFLPVDANLHNWLWLSL
                     SGLVGFTFGDYFLFRAYAEIGSRFSMLIMTTVPLFTAFLGWVFIGEKLRFLHFAGMML
                     TFIGISLAIFSRNGKGEKLTLKFAPTGILLALGGALGQATGLILSKIGMAGTYDPFAA
                     TQIRVIAGGFGFALLATFISHWPEVIEGARNREAVTAVTIGAVFGPFIGVSFSLMAVK
                     YTQAGIASTIMAIVPVLIIPPSVYLNKEKVTLAEVAGAVISVAGVALFFTG"
     gene            complement(1488..2945)
                     /locus_tag="PLG42_09330"
     CDS             complement(1488..2945)
                     /locus_tag="PLG42_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31449.1"
                     /translation="MRNTDLAAFSAVIIVYLVLFLATSHYPFFWDTVQLASKHANWFY
                     QNGFRDLLLPVDFDSGHFPVLGLYLAVCWKIFGRTLFISHLIMLPFILGIIWQTWLLV
                     KKIFSEKWKVLAFVVILSDPTLMAQCTLISPDIFLVFFFLLALNSGIFGKKKILFMVA
                     LLGLSVSSLRGMICIAAIFIADMAIKQNLFNTNSMKTIIGKIIKNLPEIITPYIPSFI
                     FIISFLTWHMVKTGWIGYHDESPWYDSFKPVDISGVIRNVFIIGWRLADFGHIFVWIT
                     GIFCFWNFLRHKIEKTESLNKLITIFLVILICLAVPMLFHKNLAGHRYLLPVYILLAL
                     TVCYCLFEITEKHMIKTVFATLMIAGLLSGNFWVYPDKISQGWDASLAYLPYPPLRKE
                     MINYMKENSIPFSETGSGFPNLAALDYIDLNGSKESFATKNLKTNKYIFWSNIFNDFS
                     DDEIDRLRKEWIIIKENKCMNVKVVLYKNPELSHN"
     gene            complement(2969..>3920)
                     /locus_tag="PLG42_09335"
     CDS             complement(2969..>3920)
                     /locus_tag="PLG42_09335"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOU31450.1"
                     /translation="IWKGGSKYGDWLFYHPPVNRHTEFDGHTEHDFIATAMYAYSTKL
                     LSLAAKELGKENDFKFYNELFNKIKEVFIHEYVTPAGRVGTCSQTSYVLALMFDLLPD
                     NLRQNAVKFLVDDIKSRRNRLSTGFVGTSYLCHVLSNMGYTDVAYDLLLQESYPSWLY
                     PVKMGATTIWERWDGIKPDTTFQDPGMNSFNHYAYGAIGDWMYRVCAGIEAGKPGYKH
                     IIIQPSPTEKLTYAKATLESRYGTIASGWEKSDNNITVRIKVPANTRATIMLPVDMKS
                     KVTEGNRPLDSVKEFTNIRVVNNKVTFEAGSGDYTFVIKI"
BASE COUNT         1229 a          778 c          730 g         1183 t
ORIGIN      
        1 attcctggtg tatctaatcc aataaaactg gggcttgcag gtggttcact cattgtagct
       61 atgtttctta gcaaattcgg acacaggttt tcccttattc cgtatacaac tacaagtgcg
      121 aaccttatgc tacgggaaac aggtatagtt cttttccttg caagtgttgg attaagagct
      181 ggagaaaaat ttatcccttc tcttacttca ggtgatggct ttatctggat gggatacgga
      241 gcaataataa ctgtgcttcc tgtaattttt accggtattt tagcaatcag atggatgaaa
      301 tataattatt ttgaagcgtg tggtttgcta gccggaagca tgaccgaccc accagccctg
      361 gcatatgcaa acaatatggc acagtcagaa atacctgcag tagcctatgc cacagtttac
      421 ccactggtaa tgtttctcag aatccttgtt gcccagttgc ttatacttct gtttgtttaa
      481 taaaaagagg caattatttt aaaagctgga atagtaaagt tcagatatag taatctctgg
      541 atcacccggt aaaaaataat gccactcctg caactgaaat tactgctcct gcaacttcag
      601 ccagtgtaac tttttctttg ttcagataaa ctgacggcgg tataataaga acaggaacaa
      661 tagccattat ggttgatgca attcctgcct gagtatattt tacagccata agtgaaaatg
      721 atactccaat aaatggtccg aacactgcac caattgtgac tgcagtaacg gcttcacggt
      781 ttctggctcc ttcaataact tctggccagt gtgaaataaa tgtcgcaagc agtgcaaagc
      841 caaaccctcc agcaattact cttatttgcg tggcagcgaa aggatcatag gtgcctgcca
      901 ttcctatttt acttaaaatt agacctgttg cctgtcccag tgcaccccca agagcaagta
      961 atataccagt aggggcaaac ttgagtgtaa gtttttctcc ctttccgtta cgactgaaaa
     1021 ttgccagcga aattccgatg aatgtgagca tcataccagc aaagtgcaga aacctcagtt
     1081 tctcccctat aaaaacccat cctaaaaaag ctgtaaaaag aggaacagta gtcatgataa
     1141 gcattgaaaa ccttgaacct atctcagcat acgccctgaa tagaaaataa tcaccgaaag
     1201 taaaacccac cagacctgaa agtgacaacc agagccagtt gtgcagatta gcatcaacgg
     1261 gcagaaagtg tcctctggct ataagattat agaatgatag cagaaatatg gcaagaaaca
     1321 ttctgataat gtttaacgaa agagaaccta tttttttgct tgccttttca tttgccagag
     1381 ctgttattgt ccggaaaaag ctgtcagaag cgctgcaaat tcgccaagat ggctgcggaa
     1441 aaatgacata atttattttt tcaaataagg atgcaaaaat aatactttta attatgggac
     1501 aactccggat ttttataaag tacaactttt acattcatac acttattttc ttttattatt
     1561 atccattctt ttctaagcct gtctatttcg tcatctgaaa aatcattaaa aatatttgac
     1621 cagaatatat atttgtttgt ctttaaattt ttcgttgcaa acgactcttt gctaccgtta
     1681 aggtcaatgt aatccaatgc tgcgagattt gggaaaccac tccctgtttc agaaaacgga
     1741 atagaatttt ctttcatata gtttatcatt tccttacgta ggggtggata tggcagataa
     1801 gccagtgaag catcccaccc ctgggaaatt ttgtcgggat atacccaaaa attaccggaa
     1861 agaagtccgg caatcatcag ggtagcaaaa acagttttaa tcatatgctt ttctgtaatt
     1921 tcaaacaagc agtaacaaac tgttagagcg agcaatatgt aaaccggaag taaataacga
     1981 tgacctgcca aatttttatg gaagagcatc ggaacagcaa ggcagatcag aatgactaaa
     2041 aatatagtta ttaatttatt aagtgattca gtcttttcaa ttttatgtct taagaaattc
     2101 cagaagcaga atatgccggt tatccatacg aaaatgtgcc caaaatctgc tagtctccag
     2161 cctatgataa aaacattgcg tattactccg gaaatatcaa ctggtttaaa tgaatcgtac
     2221 cagggtgatt cgtcatggta acctatccat cctgttttaa ccatgtgcca ggtcaaaaat
     2281 gaaattatga agataaatga gggaatgtag ggtgttatta tttcaggtaa attttttatg
     2341 atttttccaa taatagtttt catactattg gtattaaaca gattctgttt tattgccata
     2401 tcagcaataa atatagcagc tatgcatatc atacccctga ggcttgaaac tgatagccct
     2461 aacagggcaa ccatgaacaa tattttcttt tttccgaata ttccgctatt caaagccaga
     2521 agaaagaaaa atactaaaaa tatatcggga gatatgagtg tgcactgtgc cataagggta
     2581 ggatctgaaa gaatgactac aaacgccaga actttccatt tttctgaaaa tattttcttt
     2641 acaaggagcc atgtttgcca gataataccc agtataaagg gtaacattat taaatggctt
     2701 atgaagagtg ttcttccgaa tattttccag catactgcaa gatagagtcc cagcacagga
     2761 aaatgcccgc tgtcaaaatc aactggaagt aaaagatctc tgaatccgtt ttgatagaac
     2821 cagtttgcat gttttgatgc cagttgcaca gtatcccaga agaaagggta atgtgatgta
     2881 gctaaaaata ataccaggta aacaataata actgctgaaa aagcagctaa atcagtattt
     2941 ctcattaatt aactcctgat accatacttc agatttttat tacaaacgta taatctcctg
     3001 aaccagcttc aaaagtcact ttattattaa ctaccctgat atttgtgaat tctttaacag
     3061 aatccagagg cctgttacct tctgtaactt ttgatttcat atcaacaggc agcattatgg
     3121 tagctctggt atttgcagga acctttatcc ttacagtgat attattatca ctcttttccc
     3181 agccagaagc aattgtcccg tatctgcttt ctaaagttgc cttagcatat gttagctttt
     3241 cagtggggga tggttgaatt ataatatgtt tatacccggg ttttccagct tcaatgccag
     3301 cacacaccct gtacatccaa tcacctattg caccataagc ataatgattg aatgaattca
     3361 ttccgggatc ctggaaagtt gtatccggct taattccatc ccatctttcc caaattgtag
     3421 ttgcgcccat ctttacagga tagagccacg aaggatatga ctcctgcaat aaaaggtcat
     3481 atgccacatc ggtatatccc atgttcgata gaacatggca caggtaggat gttccgacaa
     3541 atccggtcga aagtctgttt ctccggcttt ttatatcatc aaccaggaat ttcacagcat
     3601 tttgcctcag gttgtcaggt aaaaggtcga acatcaatgc caggacataa gaggtttggg
     3661 aacatgtccc gacacggcct gcaggtgtaa catattcatg aatgaaaacc tccttaatct
     3721 tattaaacaa ttcgttgtag aatttaaaat cattttcttt gcccagttct tttgcggcaa
     3781 gagagagcag ttttgttgaa tatgcataca tagccgtggc gatgaagtcg tgttcggtat
     3841 gtccatcaaa ttcggtatgc ctgtttactg gtggatgata aaagagccag tcgccgtatt
     3901 tgctgcctcc tttccagata
//
LOCUS       DAOSNB010000250         3908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23338_length_3908_cov_4.147937, whole genome shotgun sequence.
ACCESSION   DAOSNB010000250 DAOSNB010000000
VERSION     DAOSNB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23338_length_3908_cov_4.147937"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1478)
                     /locus_tag="PLG42_09340"
     CDS             complement(84..1478)
                     /locus_tag="PLG42_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOU31451.1"
                     /translation="MAEKQRTIAREISLKGKGLHTGINVNITFKPAPANHGYKFCRVD
                     LPGKPVIDALAENVTEASRGTTLTQSGVSVSTIEHVLAAFYGMQVDNALIELDGPEAP
                     IMGGSSEKFVEAIKEAGIVELKEERNYFTVKQKITFSDEEHGVDLIVYPDDHFSINVL
                     IDYNSRILGNQYAILDTINDFEKEISKCRTFVFFHELEPLFKAGLIKGGDLDNAIVIM
                     EKEVPQEEIDRIAKLFNRPGINSHKAGILNNTELRYPNEPARHKLLDLIGDLALVGQP
                     IKGKVVATKPGHYANTRLARIIRQEIKKAQSKKDIPVYDPTVPPVYNYEDIKNILPHR
                     YPFLLVDKIIYVDENKVIGIKNVTGNEAFFQGHFPDEPVMPGVLMIEAMAQTGGILVL
                     NEVDDPKKYSTYFLKIDKLKFKHKVVPGDTLVIKMELTEPVRRGIVSMYGQAYVGNTL
                     VIEGEMIAQIIRNK"
     gene            complement(1579..2616)
                     /gene="lpxD"
                     /locus_tag="PLG42_09345"
     CDS             complement(1579..2616)
                     /gene="lpxD"
                     /locus_tag="PLG42_09345"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOU31452.1"
                     /translation="MEFTATVIAQFLKGEIVGNPEVKVSTVARIEEGHEGALSFLANP
                     KYEPYIYTTKSSVVLVNRDFVPAAPVQATLIKVDNAYEAFASLLTMVTQSKPARKGIH
                     PTAVVEPDAKLGENVYVGAFAYIGNGCEVGDGSQIFPHVYIGDRTKVGKECVIYPGVK
                     IYHECVIGNSCIIHAGTVIGSDGFGFAPGADNQYKKIPQIGNVVIEDDVEIGANVAID
                     RATMGSTVIRKGVKIDNLVQIGHNVEVGENTVMAGQTGIAGSTKIGNNCMIGGQVGIA
                     GHLKIADGMRACAQSGIISDIEEKNLTVMGSPAFEHRHYLKSYLLFKKLTEIKAQLDS
                     VRKEIEKLKKE"
     gene            complement(2634..3905)
                     /locus_tag="PLG42_09350"
     CDS             complement(2634..3905)
                     /locus_tag="PLG42_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU31453.1"
                     /translation="MNFSHVDYSTNKRKIINDPVYGFINIPGDFIFDLIQHPWIQRLR
                     NIKQLGLTNYVYPGATHTRFQHCLGALHLMDTALQTLKGKGVFISPEEEEAALVAILL
                     HDAGHGPFSHALEKSIINGITHEDLSLLLMKMLNEEFRDRISLAIDIFTGKYRRKFFH
                     ELITGQTDMDRLDYLRRDSFYTGVIEGSVGSDRIIRMLNVADDQLAIEEKGIYSLEKL
                     LIARRLMYWQVYMHKTVLSSESLLVNMLKRAKEIARKGEQLYATPSLSFFLYNEIGKD
                     DISGKGTFPPAVVAFHFTRLDDSDIFVSARYWNDHSDRVLSDLSSRLIRRNLFAVELQ
                     NEPFPPERIESLQKKAEKMLDIEPEMVNYYVFSGKVSNLAYAPDGSSRVKILLKSGLT
                     AKIEEVSDIFGQSFLSERIVKYFLCYPKECR"
BASE COUNT         1058 a          878 c          700 g         1272 t
ORIGIN      
        1 tatatgcaaa tggttctacg actacgtttt cgccaagttt agcatcagga tgaacaaaag
       61 ccaattttga gatcataatc ttttcatttg tttcttataa tctgagctat catttctccc
      121 tcaattacaa gggtatttcc aacgtatgcc tgtccataca tcgaaactat tccccttctg
      181 acaggctcgg ttaattccat tttgatcacc agagtatctc ctgggactac tttatgcttg
      241 aatttaagtt tatcaatttt cagaaaatat gtagaatatt ttttgggatc gtcaacttcg
      301 ttcagtacca gaataccgcc agtttgagcc atagcttcta tcataagaac tcccggcata
      361 acaggttcat caggaaaatg cccctggaaa aatgcttcat ttcctgtgac attctttata
      421 ccaataacct tgttctcatc aacataaatt atcttgtcaa caagtaaaaa aggatatctg
      481 tggggcagta tattctttat gtcctcgtaa ttatacacag gtggtacggt tgggtcataa
      541 acaggtatat cttttttcga ctgagccttt ttgatttcct gtcttataat tcttgccagc
      601 ctggtattgg cataatgtcc aggttttgtt gccaccactt tgccttttat cggctgccca
      661 acaagtgcaa ggtcacctat caggtcaagc agtttgtgcc tggccggctc atttggatat
      721 cttaactcgg tattattcag aatacctgct ttatggctgt ttatgccagg cctgttaaag
      781 agctttgcaa ttctgtcaat ctcttcctgc ggcacctcct tttccattat cacaattgca
      841 ttatccaggt cgccgccttt tatcaaaccc gccttaaata aaggttcaag ttcgtggaag
      901 aatacaaaag tacggcattt gcttatctct ttttcaaaat catttattgt atcgagaatt
      961 gcatactggt taccaagtat tcttgaatta taatcaatga gaacatttat gctgaaatga
     1021 tcgtcgggat aaacaatcag gtcaacacca tgctcctcgt ctgaaaatgt aatcttctgt
     1081 ttaacagtaa aataatttcg ctcttccttc agttcaacta ttcctgcttc ctttattgct
     1141 tcaacaaatt tttccgatga acctcccata ataggagctt caggtccatc aagttctata
     1201 agggcgttgt caacctgcat accatagaat gctgcgagaa catgttcaat ggttgaaaca
     1261 gatactccgc tttgagttaa tgtggtacct ctcgaggctt ctgtaacatt ttcggcaaga
     1321 gcatctataa caggcttgcc cggaaggtca accctgcaga acttgtatcc atggttagct
     1381 ggcgctggtt taaatgtaat atttacattt attcctgtat gcaatccttt ccccttaagg
     1441 cttatttcac gcgcaatagt tctttgcttt tcagccattt cagtcaaaat atgtaattaa
     1501 aatctgttac aaaggaaata aaaatttcgt gaactattta tgaaaattga cattaatgct
     1561 tatcctcaac aattacggtt attccttttt cagtttttca atctcttttc tcactgaatc
     1621 gagctgggct tttatctctg taagtttctt aaataacagg tatgacttta gataatgacg
     1681 atgttcgaat gcgggggaac ccattacagt aaggttcttc tcctcaatgt cgctgatgat
     1741 acccgattgt gcgcaagctc tcattccatc ggcaattttg agatgtcccg ctatcccaac
     1801 ttgcccccct atcatacaat tgttcccaat ttttgtagac cctgctattc ctgtctgacc
     1861 tgccattact gtattttccc cgacttcaac attatgtcct atctgcacaa gattgtcgat
     1921 cttaacacct tttctgatga cagtcgagcc cattgttgcc ctgtcgattg caacgttagc
     1981 tcctatctcc acatcatctt caataaccac attgcctatc tgaggtatct tcttgtactg
     2041 attgtctgcc ccgggagcga acccaaagcc gtcacttccg ataacagttc cggcatgtat
     2101 gatacatgaa ttacctatta cacactcatg gtaaattttg acaccgggat agattacgca
     2161 ttccttacct acttttgttc tatcgccgat atacacatgt ggaaaaatct gactgccatc
     2221 cccaacttca cacccattac caatataggc aaaggcaccc acgtaaacat tttctcccag
     2281 ttttgcatcc ggttcaacta cagcagttgg atgtatgcct tttctggcag gtttagattg
     2341 tgttaccatt gtcagcagtg atgcaaaagc ctcataagcg ttgtcaactt ttatgagtgt
     2401 tgcctgaaca ggggccgcag gtacaaaatc cctgttgaca agcactactg atgactttgt
     2461 tgtgtaaata taaggttcat atttagggtt tgccagaaac gacagggccc cttcatggcc
     2521 ctcctcaatt ctggcaacag tactaacctt tacttcagga ttcccgacta tttcaccttt
     2581 gagaaactga gctattacag ttgctgtaaa ttccataatt cattcatttt aaattatctg
     2641 cattcttttg gataacaaag aaaatattta actattcttt ctgacagaaa actctggcca
     2701 aagatatcag aaacttcttc gatttttgct gttaatcccg atttcagcag aattttcacc
     2761 ctggaactgc cgtcgggagc ataggcaaga tttgagactt tccctgaaaa tacataataa
     2821 ttaaccattt ccggttctat atcaagcatc ttttcagctt tcttctgtaa actttctatc
     2881 ctttccggag ggaatggttc attctgcagt tctactgcaa aaagatttct gcgtataagc
     2941 ctcgaggaaa gatctgaaag tactctgtcg gaatggtcat tccagtatct tgccgaaaca
     3001 aatatgtcgc tgtcgtccag tcttgtaaaa tgaaaagcca caactgcagg tgggaaagta
     3061 ccttttccgc tgatatcatc cttaccgatt tcattataga ggaagaaact cagagatgga
     3121 gttgcgtaga gctgttcacc tttgcgggca atctcttttg cccttttaag catatttact
     3181 agaagactct cagaagaaag tacagttttg tgcatatata cctgccagta catcaacctg
     3241 cgggcaatta atagtttctc aagagaataa attccttttt cttcaattgc cagctggtca
     3301 tcagccacat tcagcatcct gattatcctg tcggaaccca ccgagccttc tattactcct
     3361 gtataaaagc tgtctctcct caggtaatcg agacgatcca tgtcggtctg tcccgtaata
     3421 agttcatgaa agaactttct tctgtatttt ccagtgaata tatcaatcgc aagcgaaatt
     3481 ctgtccctga attcttcatt aagcattttc atcagaagta atgaaaggtc ctcatgagtt
     3541 atgccgttaa ttattgattt ttcaagggcg tgtgagaagg gaccatggcc agcatcgtgg
     3601 agaaggatag caacaagcgc tgcctcttct tcttcaggac ttatgaaaac acctttacct
     3661 ttcagtgtct ggagtgcagt gtccattaaa tgcagggctc ccaggcaatg ctgaaacctg
     3721 gtgtgagtgg caccgggata tacataattt gtaagaccca gttgcttgat gtttctcaat
     3781 cgctgtatcc agggatgctg gataagatca aagatgaaat caccgggaat attgataaag
     3841 ccataaaccg gatcatttat tattttcctt ttatttgtac tgtaatccac gtgggaaaag
     3901 ttcacagc
//
LOCUS       DAOSNB010000251         3901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23386_length_3901_cov_4.691628, whole genome shotgun sequence.
ACCESSION   DAOSNB010000251 DAOSNB010000000
VERSION     DAOSNB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23386_length_3901_cov_4.691628"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLG42_09355"
     CDS             complement(<1..608)
                     /locus_tag="PLG42_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOU31454.1"
                     /translation="MNPIRSNGARLRQILGLNSYPVGVKFLWADDKIKQKAEKLTGYR
                     YCQALMKARHGEHVILDAEGIACPAAAAAFGFKQLPEGLKTGKGLVGFGIVNQEATGK
                     VMFEGMTTLPMGKLKALYLFPLDTANIEPDIVVIEDETEKLMWIALANLNVKGGKRIE
                     SSTAILQATCVDATLIPYVEQKFNISMGCYGCRDATDIGNNET"
     gene            complement(620..862)
                     /locus_tag="PLG42_09360"
     CDS             complement(620..862)
                     /locus_tag="PLG42_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU31455.1"
                     /translation="MLEIKVLGTGCPNCKRLEDMCREVVAENNLDATIEKVSNIDEFW
                     KYGVMLTPALIVNGKLLVQGKLPIRHTLVHWLMENK"
     gene            complement(874..1200)
                     /locus_tag="PLG42_09365"
     CDS             complement(874..1200)
                     /locus_tag="PLG42_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOU31456.1"
                     /translation="METKKTPMELLKEFAPEFAQHQMNDKALLFENEKYQAVPKKYKL
                     LAGIAVAAALGSDTCVRMWVKQARDAGITTPEIVEAIMVARYMKQAVVNDTVANTLAL
                     ISENKL"
     gene            complement(1220..2563)
                     /locus_tag="PLG42_09370"
     CDS             complement(1220..2563)
                     /locus_tag="PLG42_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOU31457.1"
                     /translation="METKKELKILGWIIIVFAVAFFLPIESTRFNTAVSATFDLVKWY
                     AREHVILCLLPAFFIAGVISVFISQASVIKYLGANARKWVAYTVASVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGFEMGVARTIGAVAFSII
                     IGSIMAIIYRKEEKAKKEEQMNIVPSQEKRPMWQTSLHFFTLVLILVFANWGKPAEGD
                     TTSLWYSIWQYKWWITGFFSLVLGYSLIKILKIKWQWVLGAALVVIASVILSNNLIES
                     QKLRPLVPMLVGIIALSIITIFDKVDEENKAWTLATWDFAKQILPLLATGVVIAGFLL
                     GSTHDGKTIPGIIPNEWVTALVGGNSVFSNFFASIVGAFMYFATLTEVPILQGLIASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVIGTQKTVVYVSLVVIMATITGLIYGSIF"
     gene            complement(2646..3221)
                     /locus_tag="PLG42_09375"
     CDS             complement(2646..3221)
                     /locus_tag="PLG42_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOU31458.1"
                     /translation="MSISSTDTGSPVSDRIEIYYFHFTMRCATCLTIEAKTKEYLEEL
                     YPDHLRAGLITFKALNIEEEVNKSVAERFGITGQALIIVKGKKKWSYLDAMLLGLLFA
                     LAFCPYSGVLYFGMLIPMTVASSSGLYLPVIFAIATGIPVIIFAWIIAYTVSGIGSVY
                     NKLKNFEFWFRRTVAVLFIVTGIYYSIKMYL"
     gene            complement(3310..3549)
                     /locus_tag="PLG42_09380"
     CDS             complement(3310..3549)
                     /locus_tag="PLG42_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOU31459.1"
                     /translation="MNIKILGTGCPKCKTLENVTLEALQELDVNASVEKVEDIMKILS
                     YNVLHTPALVINEKIVISGQVPGVNQVKELILKNQ"
     gene            complement(3618..3893)
                     /locus_tag="PLG42_09385"
     CDS             complement(3618..3893)
                     /locus_tag="PLG42_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOU31460.1"
                     /translation="MAKIFKALSHPVRIYIIKKLIKMDACCYSGDLVEELPIGRSTLS
                     QHLKELKYAGMIQGEINPPYIKYCLNRKNWEKAKAIIEKFLNENQHF"
BASE COUNT         1153 a          828 c          718 g         1202 t
ORIGIN      
        1 gtttcattat taccgatatc cgttgcatcg cggcatccat agcaccccat acttatattg
       61 aacttctgtt ctacatatgg aatcagggtt gcatcaacac aggtcgcctg caaaattgct
      121 gtactgctct caattcgctt ccctccctta acgttcagat tggcaagggc gatccacatt
      181 agtttttcgg tttcatcttc aattactact atgtcaggtt caatatttgc agtgtcgaga
      241 gggaagaggt ataatgcttt aagtttaccc attggaaggg tagtcattcc ttcgaacata
      301 accttgcctg tagcctcttg gtttacgata ccaaaaccga caagcccttt acctgtcttc
      361 aaaccttcgg gcagttgttt aaatccaaag gcggccgcgg cagccggaca ggcaatgcct
      421 tcagcatcaa gaatcacatg ttcaccgtgc cttgccttca taagagcctg gcaataacgg
      481 taacccgtca atttttctgc cttctgctta attttgtcat cagcccataa gaattttact
      541 cctacagggt atgaattcaa accaagtatt tgtcttaaac gggctccatt agatcttatg
      601 ggattcattc ttctgacttt tatttatttt ccatcaacca atggaccaaa gtgtgtctga
      661 tcggtaattt accctgtaca agaagttttc cgttaactat aagggccggt gtaagcatta
      721 ctccgtattt ccagaactca tcgatatttg aaaccttttc gattgtggca tccagattgt
      781 tttcagcaac gacctcccgg cacatatctt caagtctttt gcaattgggg cacccggttc
      841 cgaggacttt aatttccagc atacaaatag gctttataat ttattttccg aaattagagc
      901 aagagtgttt gcaacagtgt catttaccac tgcctgtttc atatatctgg ctaccataat
      961 agcttcaact atttcaggag tggtaattcc agcatccctt gcctgtttta cccacattct
     1021 cacgcaggta tctgaaccca gagcagctgc aactgcaatg cctgccagaa gcttatactt
     1081 tttcggaaca gcctggtatt tttcgttctc gaaaagaaga gctttgtcat tcatttgatg
     1141 ctgggcgaat tcaggagcga attccttcag caattccatg ggtgtctttt ttgtttccat
     1201 aacataatgg tattagatat taaaaaattg aaccatatat caaaccggta attgtagcca
     1261 ttataaccac aagacttaca taaactacag ttttctgggt tcctatgact cctcttatta
     1321 cgagcatatt cggcaatgaa agtgacggcc cggcaagcaa tagtgcaaga gcaggacctt
     1381 ttcccattcc cgatgcaata agtccctgaa gaataggcac ctcggttaaa gtggcgaaat
     1441 acatgaatgc ccctacaata gatgcaaaga agtttgaaaa caccgaatta ccacctacca
     1501 gagcagtaac ccattcgttg ggaataatac caggaatggt tttcccatcg tgagttgaac
     1561 ccagcagaaa tcctgcaata acaactcctg ttgcaagtaa aggaagtatc tgttttgcga
     1621 aatcccatgt tgccagtgtc catgctttgt tttcttcatc aaccttatca aaaatcgtaa
     1681 ttatgcttaa tgcaattatt ccgacaagca ttggtacaag aggcctgagc ttctgacttt
     1741 caatcagatt attcgacagg ataacagatg caataacaac taaggcagca ccaagaaccc
     1801 actgccattt tattttgaga attttaataa gcgaatatcc aagtacaagg ctaaagaagc
     1861 ctgtaatcca ccatttatat tgccagatgg agtaccacaa acttgttgta tcaccttcgg
     1921 caggtttgcc ccagtttgca aaaacaagta tcagaacgag ggtaaagaag tgcagtgatg
     1981 tctgccacat aggccttttc tcctgtgagg gaacaatgtt catctgttcc tctttctttg
     2041 ccttttcttc tttacgataa ataatagcca ttattgagcc gattatgata ctgaatgcaa
     2101 ccgcacctat ggttctggca acccccattt caaagcccag aattcttgca gtaagtatta
     2161 tggcaagaat attgattgca ggacctgagt aaaggaatgc tattgccggt ccaagacctg
     2221 cccctctttt atggatactt gaaaacagag gaagtattgt acatgagcaa acggcaagta
     2281 ttgtacctga tactgaggcc accgtatagg caacccactt cctggcatta gcccccagat
     2341 atttgataac agatgcctgg cttatgaaaa cagaaataac cccggctata aagaatgcag
     2401 gaagaagaca taaaatgaca tgttcccgtg cataccattt gacaagatca aatgtggcag
     2461 aaacagcagt gttaaaacgg gtgctttcaa taggaagaaa gaatgcaaca gcaaacacta
     2521 taataatcca gccaagtatt ttaagttctt tctttgtttc catctttttt attcaaaagt
     2581 ttaatgttta ttaacgggat gatttcagtt tgttattttc caaaatcaaa atgactaata
     2641 aaattttata agtacatttt tattgaataa tatattccgg ttacaataaa caagactgca
     2701 acagttcgcc tgaaccagaa ttcaaaattc ttaagtttat tgtacactga gccaattcct
     2761 gaaactgtat aggcgataat ccatgcgaaa attattactg gaattcctgt ggcaattgca
     2821 aatattacag gaagatatag tcctgaagag cttgcaactg tcataggtat cagcattcca
     2881 aaatagagca caccgctgta agggcagaaa gcaagggcaa aaagcaagcc gagtagcata
     2941 gcatcaaggt aactccattt ctttttgcct tttacaataa taagagcctg acctgtaata
     3001 ccaaatctct ctgcaaccga tttatttact tcttcttcta tattgagggc cttgaaagta
     3061 ataagccctg ccctgagatg atcaggatag agctcttcaa gatattcctt tgttttagcc
     3121 tcgatagtca gacatgtagc acaccgcatt gtaaaatgaa agtagtatat ttcaatcctg
     3181 tcagaaaccg gggagccggt atctgtggat gaaattgaca tggttttggg ctgggaattg
     3241 cacgagccca gaagtatagt caattcaata ataatgaagc tagcaatttt ttatctgctt
     3301 tcatttaaat tattgatttt taagaattaa ttctttaacc tgatttactc cgggcacctg
     3361 cccgcttatt acaatttttt cgtttattac aagagccgga gtgtgcaata cattgtaact
     3421 caaaattttc ataatatctt caactttttc aacagaagca ttgacatcaa gctcctgcag
     3481 agcctcaagt gttacatttt cgagtgtttt acatttaggg catccggttc cgagaatctt
     3541 tatattcata gcttaagtat taaaggattt aacaaattat ttgtcagagt aattattttt
     3601 attcttcaca gtagatctca aaaatgttga ttttcgttaa ggaacttttc aattattgct
     3661 tttgctttct cccagttctt tctgtttaaa cagtacttaa tgtacggagg attaatttct
     3721 ccctgaatca ttcctgcata ttttagttcc ttaagatgct gggataaagt ggacctaccg
     3781 ataggaagct cttcaaccag atctccgctg taacagcagg catccatttt tataagcttc
     3841 ttgattatgt aaatcctcac ggggtggctt aaggccttga atattttagc cagctcgagg
     3901 a
//
LOCUS       DAOSNB010000252         3872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23591_length_3872_cov_4.276919, whole genome shotgun sequence.
ACCESSION   DAOSNB010000252 DAOSNB010000000
VERSION     DAOSNB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23591_length_3872_cov_4.276919"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLG42_09390"
     CDS             complement(<1..1164)
                     /locus_tag="PLG42_09390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOU31461.1"
                     /translation="MNSIRTLLNVIVVSILTFLPGCTVPFDREAKKPAEMVNPLMGTD
                     SDYRLSHGNTYPAVAMPWGMNFWTPQTGRMGNGWIYTYDATKINGIKQTHQPSPWIND
                     YAAFALMAVTGKEAYREEERASWFSHKAEKAKPYYYSVYLADYDVTVEVTPTTRAAIF
                     RFTFPESDSSYIILDAFNMGSGVKIIPGERKITGYCRNNHGGVPENFTNWFVAVFDRD
                     FDSYATWRNDTLRRDSLSAEGEHSGALVRFKTKRGDKINVRVASSFISAEQAMLNLER
                     EIGGKDFEQVKEECRSEWNRQLGKITVEGGTDDQIRTFYSCLYRMLLFPRDLHEINEK
                     NEKVHYSPYNGEVLPGEMFTDNGFWDTFRAQFPFLTLVFPGLNSRIMNGLVNTY"
     gene            complement(1241..2284)
                     /locus_tag="PLG42_09395"
     CDS             complement(1241..2284)
                     /locus_tag="PLG42_09395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOU31462.1"
                     /translation="MRILVIRLSAMGDVALTLPAVKSFVERYPGHELTILTRPAFSTF
                     FRGIPTLRLFTPDLNHYKGLYGIWKIYRDLAGSGRFDMVIDLHDVIRSKILRCFFRIA
                     GTPVFSIDKGRREKRGLVSGRERKLLRHTVERYADVFRRAGFELALSAGKFIDAGSEA
                     RKKVDTLFSTSTLLNIGIAPFARHELKQWPVEYMVSLMRMISGTVSARFWLFGGTDEC
                     ERLAGIEKVTERSVNLCGKLSLEEEIAVMERLDFMIAMDSANMHMAALVGTKAVSIWG
                     GTDPLAGFGAWGQPPEYSIRISPSELECRPCTIYGKGRCKRKDLACLYRLTPEIVFGE
                     IVKLGLLKEKNRI"
     gene            complement(2284..2655)
                     /locus_tag="PLG42_09400"
     CDS             complement(2284..2655)
                     /locus_tag="PLG42_09400"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HOU31463.1"
                     /translation="MKIEVSNGEIIDKLTILMIKLERIKDEEKLANIRKEYEMLREAA
                     ELVISLDDPLVKALYQVNSELWDIEDHIRELERRKDFGDDFINTARSVYFKNDRRAEL
                     KREINIKTSSGLIEEKSYEKY"
     gene            complement(2772..>3872)
                     /gene="priA"
                     /locus_tag="PLG42_09405"
     CDS             complement(2772..>3872)
                     /gene="priA"
                     /locus_tag="PLG42_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOU31464.1"
                     /translation="KAGKYGFAELKERYGKVNLPEIILVNTREAYRKKLMISHFSPQL
                     YEAIEEALKKKEQVLLFRNRRGFSPYLQCAECGWIPSCENCAVKLTYHKEINRLVCHY
                     CGRAKSVPQKCDMCGSSLLETRGFGTEKIEEEIKMLFPGARVARMDHDTTRRPDSFSR
                     IIKDFEAHRIDVLAGTQMISKGLDFENLTVVGIMNADSLLNYPDFRSYERAFQLMAQV
                     SGRAGRRLKRGKVIIQTSDPEHRIMRLVLRNDYRGFYESQLEERKTFNYPPFCRLVKI
                     ILKHRNKKILDEFAGFLADILRKEFGKGVLGPEYPVISKIQLWYLKEILVKINRDRSL
                     QASKDAIRQAIEKTRELKGAATLRIVTDVDPY"
BASE COUNT         1013 a          942 c          710 g         1207 t
ORIGIN      
        1 gtacgtattt accaatccgt tcattatcct gctgttaagc ccgggaaaca caagtgtaag
       61 gaacggaaac tgcgctctga aagtgtccca gaaaccgtta tccgtaaaca tctcaccggg
      121 gagaacctcg ccgttatacg gactataatg gactttttcg tttttctcat ttatctcgtg
      181 aaggtctctt gggaagagta gcattctgta caggcacgaa tagaacgtcc ttatctgatc
      241 gtcagtacca ccttcaacgg taatttttcc cagctgtctg ttccattctg acctgcactc
      301 ttccttaacc tgctcgaaat cctttccgcc aatctctctt tcaagattta acattgcctg
      361 ctcagcactg ataaacgatg atgctacacg cacatttatt ttatctcctc tttttgtttt
      421 gaacctgaca agggcacctg aatgttcacc ttcagccgac aggctatccc tcctgagcgt
      481 atcattgcgc caggttgcgt atgaatcaaa atccctgtca aagactgcaa caaaccagtt
      541 ggtgaaattc tccggaacac caccatgatt gttcctgcag tatccggtga ttttcctttc
      601 accgggaatt atcttcacac ccgaacccat gttgaatgca tcgagtatga tatatgacga
      661 atcactttca gggaatgtaa agcggaaaat tgctgcgcgt gttgttggtg ttacttcaac
      721 tgtaacatca taatcggcaa gataaacgct ataataataa ggttttgcct tttcagcttt
      781 atgggaaaac cacgaagccc tctcctcctc cctgtaagct tcctttcctg taacagccat
      841 cagtgcaaag gcggcataat catttatcca ggggcttggt tgatgggttt gttttattcc
      901 attaattttt gttgcatcgt aggtatatat ccagccgttc cccatacggc ctgtttgagg
      961 tgtccagaaa ttcatacccc acggcattgc aacagcgggg taagtattac catgtgataa
     1021 acggtaatca gagtcggttc ccattaaagg atttaccatc tcagcaggtt tcttagcctc
     1081 tctgtcaaaa ggcaccgtgc aaccaggtaa aaaggtcaga atgcttacaa caatgacatt
     1141 taataaagtt cttattgaat tcatatcatt aattcttttt tcttatttat ctgatttaaa
     1201 caaagttatt aaagaacttt taacattaat actattactt ttatattctg tttttttcct
     1261 taagaagacc gagtttaacg atctccccga acacaatttc aggagtaagg cggtagaggc
     1321 aggcaagatc tttccttttg cacctgcctt ttccataaat tgtacatggt ctgcattcga
     1381 gttcagaagg cgatatgcgt attgagtatt caggtggctg cccccatgca ccgaaaccgg
     1441 caagcggatc agtaccaccc catattgaaa ctgctttagt cccgacaaga gcagccatat
     1501 gcatgtttgc agaatccatg gcaatcataa agtcgagcct ttccataaca gcaatttcct
     1561 cttcgaggga aagcttaccg caaaggttaa cactcctttc cgtgaccttt tcaattcctg
     1621 caagcctttc acactcatca gttccaccga aaagccagaa cctggcacta actgttccgg
     1681 aaatcattct catcagacta accatatatt ccactggcca ttgtttcagc tcatgccttg
     1741 caaaaggagc tataccgata ttcaaaagtg tgctggttga aaatagcgtg tcaacttttt
     1801 ttcttgcttc agaacccgca tcaataaatt tccctgcgga aagagctaac tcaaaacctg
     1861 ccctccggaa tacatcagca tacctctcaa cagtgtgtct caacagtttt ctctcccttc
     1921 ccgacaccag tcctcttttt tcccttctgc ctttgtcaat agaaaaaacc ggagtcccgg
     1981 caatgcgaaa aaaacacctc agaatcttac ttcttataac gtcatgaaga tcaataacca
     2041 tatcaaatcg tccggaacct gccagatctc tgtagatttt ccatatcccg tacaaccctt
     2101 tataatgatt caggtctggg gtgaaaagcc ttagtgtggg tattcccctg aaaaaggtgc
     2161 tgaaagctgg ccgggtaaga attgtgagtt catggccggg atatctttct acaaatgatt
     2221 taacggcagg caatgtcaaa gcaacatcgc ccattgcaga aagacgtatg acaagaatac
     2281 gcatcagtac ttttcgtaag atttctcctc aatcaggccg gaagatgttt ttatgtttat
     2341 ctcccgttta agctcagctc ttctgtcgtt tttgaaatat accgaacgag ccgtgttaat
     2401 gaaatcatcg ccaaaatctt ttctcctctc aagctcccgg atgtgatcct cgatatccca
     2461 gagttcactg ttgacctgat acaaagcctt tacgagtgga tcgtcgaggc tgattactaa
     2521 ctcggctgct tcgcgaagca tttcatattc ttttctgatg ttggctaact tttcctcatc
     2581 cttgatcctt tcgagcttaa tcatcagaat ggttagttta tctattattt ccccgttcga
     2641 tacttcaatt ttcataaatt cttatttctt tcaaaagtag gaatttcaga agaattaagc
     2701 gcactatgat ttctgtgatt tttgccagat aatattcaaa tttttatcca gtcccggagt
     2761 agtagtatag cttaatacgg gtcaacatcc gttacaatcc tgagtgttgc tgcgcctttc
     2821 aattccctgg tcttttcgat agcctgcctg attgcatctt ttgatgcctg tagtgacctg
     2881 tctcgattaa ttttcacaag tatctcctta aggtaccaca attgaatttt tgatattacc
     2941 gggtattccg gtcccaatac gcctttaccg aattcttttc tcagaatatc agcgaggaat
     3001 cctgcaaact catccaggat ttttttattc ctgtgtttga ggattatttt caccagccgg
     3061 caaaaaggag ggtaattgaa tgtttttcgt tcttcaagct gcgactcata gaatcccctg
     3121 tagtcgtttc tgagcaccag tctcataatt ctgtgctccg ggtcggatgt ctgaattatt
     3181 actttacccc ttttaagtcg tcgtcctgcc ctgccactta cctgtgccat gagctgaaat
     3241 gccctttcat atgacctgaa atcaggatag ttcagcagac tgtcggcatt catgatgccg
     3301 accacagtaa ggttctcaaa atcgagtcct tttgatatca tctgggtacc ggcaagaaca
     3361 tctatgcggt gtgcttcgaa atcttttatt attctgctga aagagtcggg tctgcgtgtt
     3421 gtatcatggt ccatcctggc tactcgtgca cctggaaaca acatctttat ctcctcctca
     3481 atcttttccg ttccaaatcc tcttgtctcc agcaatgaag aaccacacat gtcacacttt
     3541 tgcggtacac tctttgcccg tccacagtaa tgacacacca gccggtttat ttctttatga
     3601 tatgtcagtt ttacggcgca attctcacag gaaggaatcc atccacattc agcacactga
     3661 agatatggtg aaaaacccct cctgttgcgg aaaagaagaa cctgctcttt ctttttcagt
     3721 gcctcctcaa ttgcttcata cagctgaggg gaaaaatgtg aaatcattaa cttctttctg
     3781 taagcttcac gcgtatttac aagaataatc tccggtaggt ttacttttcc atagcgctct
     3841 ttcaattcag caaatccata cttccccgct tt
//
LOCUS       DAOSNB010000253         3865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23650_length_3865_cov_4.436483, whole genome shotgun sequence.
ACCESSION   DAOSNB010000253 DAOSNB010000000
VERSION     DAOSNB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23650_length_3865_cov_4.436483"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..421
                     /locus_tag="PLG42_09410"
     CDS             164..421
                     /locus_tag="PLG42_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOU31465.1"
                     /translation="MPESRFEIQICLGSSCFSRGNKNLVKIIQEYLKKNHIDDKVVFK
                     GSRCMGYCSEGPNLVINGKIIKGINAAEAEAILDRELQNIK"
     gene            434..2314
                     /locus_tag="PLG42_09415"
     CDS             434..2314
                     /locus_tag="PLG42_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU31466.1"
                     /translation="MHNSSPVLYINEEKCRNSYACVRVCPVNAIEVKPQRKHPYILPD
                     KCIGCGLCYISCSAKAVEFRDSRQEVKELLASPRKTAALVAPSIASEFDDITDYRKFV
                     SMIRALGFDYVHEDSFGVDLVARKYAELFEKAKGKYYITANCPVIVKLIEKYFPGLVP
                     NLAPIVSPMAATAMVVKDLYGPDVATVFIGPCIEAKDEKFLYSSGKLVDSVLTFIELR
                     QLFEEAGIQERLVPMSEFDPPHGNWGALYPLAPGIIQAAGIRRDMVTSSIITASGKEE
                     IFEAISDFDRYIDYILHHFNLFFCHGCLLGPGMENHTERFRRRALVRKYTQKRVDLLD
                     KKKWEEDMKRWSKLNLTATFRADDRRVPDPPEDKIKEVLNIITGGNNEEYLNCSVCGY
                     KSCRDFAITVAKGLAIPEMCHVYNIRNKQEYIESLRQTNRKLAETKKALKESEEAARK
                     EKEAAQDASDMLHNMLEKLPSGVVIVDSNLKIMHSNQSFINIAGEDAKAIAEIIPGLA
                     GADIKTLLPFNVYNMFTYVLREDQPVLNKDIKYDDRMLNISIFPIRKNKICGAIIRDL
                     YSPEVYSEEVTARISEVIEKNLEMVQKIGFLLGEGAAETEQMLNSIIEFYKKVKTRSE
                     SS"
     gene            2326..3510
                     /locus_tag="PLG42_09420"
     CDS             2326..3510
                     /locus_tag="PLG42_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOU31467.1"
                     /translation="MAKDFFIEINSQQRNHDGQRICGDVFLYKYVQEEDRVIAVLSDG
                     MGHGVKANMLATLATTMALNFTREHKDVNRIAEIIMNTLPVCSQRKISYATFTIIDIE
                     SNGRISILEYDNPPVIILRGSNYIDIDWSTIKLEKGKHAGKSLRKYTFIPKKEDRIII
                     CSDGVTQSGMGSDTYPFGWERENVIKYATGLIKGDHSISADALANKIVTMANKNDNYR
                     PQDDISCAVIYFREPRKLLLCTGPPYEKEKDRELAKKVEEFKGKVIVCGGTTADIIAR
                     ELNRPITDNLVFEDPELPPESFIQGIDLVTEGILTLQKVNEILKTYNNSVRLGKGPAD
                     KIVKLFMESDEIHFIIGTRINIAHQDPNLPVDLEIRRTVVKRIARLLEEKWLKKTTFE
                     YI"
     gene            complement(3482..>3865)
                     /locus_tag="PLG42_09425"
     CDS             complement(3482..>3865)
                     /locus_tag="PLG42_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU31468.1"
                     /translation="TYSVIGCRYRGRWVFVRHRGKHTWEMPAGHVEEGETPEEAACRE
                     LAEETGAVLFDIHCVATYSVEEQGVTGYGKLYFAEIKKMENFSDREEIEEVIFSADMP
                     SALTYPVIQPVLFSQVVRYTQKLSS"
BASE COUNT         1234 a          833 c          851 g          947 t
ORIGIN      
        1 ttattaatta tttaaaattt tattcaaatc tggtatttga tttttaattt aatccttgaa
       61 tgaaaattat taataatttt tcagcaaaca atataagata aaattatgag tgctttaaaa
      121 ttatgattat ctttggttgt attgttcaca aataaacaaa acaatgccgg aaagcaggtt
      181 tgagattcag atttgtctgg gaagcagttg cttttcacgc ggaaacaaga atctggtaaa
      241 aataattcag gaatatctta aaaagaatca tattgatgat aaagtagttt tcaagggttc
      301 aaggtgtatg gggtattgca gtgaagggcc caacctggta ataaacggta aaattataaa
      361 aggcattaat gccgctgagg ccgaagcaat actcgaccgt gaactacaga acattaaata
      421 aaactccatt aaaatgcata actcctcacc ggttttatat attaacgaag aaaaatgcag
      481 gaattcctat gcatgcgtaa gggtttgtcc tgtgaatgcc atcgaggtaa agcctcagag
      541 aaagcatccg tacattcttc cggacaagtg tattggctgc gggttgtgtt atattagttg
      601 ctcggcaaaa gctgttgagt tccgcgattc acgccaggag gtaaaagagc tgctggcttc
      661 accacgcaaa actgcagcgc tggtagcacc cagcattgct tctgaatttg acgatataac
      721 agactaccgt aaattcgtca gcatgatcag ggcactaggc ttcgactatg tgcatgaaga
      781 ttctttcgga gtagatctcg ttgcccgcaa atacgccgag cttttcgaaa aagcgaaagg
      841 gaaatattac ataactgcca actgcccggt aattgtaaaa cttatcgaaa agtattttcc
      901 gggactcgta cccaatctgg cgcctattgt ctcacctatg gcagccacag cgatggtcgt
      961 aaaggatttg tacggaccag atgtggctac cgtatttata ggaccgtgta tagaagccaa
     1021 ggacgagaaa tttctttact catcgggcaa acttgtcgac agtgtcctga catttattga
     1081 actacgccag ctctttgagg aagcgggtat tcaggaaagg ctggtcccga tgtcggagtt
     1141 cgacccgccc catggcaact ggggagcact ctacccgctt gccccgggaa tcatccaggc
     1201 tgccggtatc aggcgcgata tggtgacaag cagcataata accgcatcgg gcaaagaaga
     1261 aatattcgag gcaataagtg atttcgaccg ctacatcgat tatatcctcc accatttcaa
     1321 cctcttcttc tgccatggat gtctcctcgg tccgggtatg gagaaccaca ccgaaaggtt
     1381 caggcgcagg gctcttgtaa gaaaatatac acagaagagg gttgacctgc tcgacaagaa
     1441 aaagtgggag gaagatatga agagatggtc gaaactgaac cttactgcaa ccttcagagc
     1501 cgatgaccgg cgtgtacctg acccaccaga ggataaaata aaggaagtcc tcaatatcat
     1561 cacaggaggt aacaacgaag agtacctcaa ctgcagcgta tgcggatata aatcgtgccg
     1621 cgattttgca ataaccgttg ctaaaggact tgctatcccc gaaatgtgtc atgtgtacaa
     1681 cataagaaat aagcaggaat acattgaatc gctcaggcag acaaacagga aactggctga
     1741 gaccaagaaa gcccttaagg aatcggagga agctgcaaga aaagaaaaag aagcagcaca
     1801 ggatgcatct gacatgcttc acaatatgct cgaaaaactg ccatcgggag tggtaattgt
     1861 cgacagcaac ctgaaaataa tgcactcaaa tcagagcttt ataaatattg ccggagaaga
     1921 tgctaaagcc attgccgaaa ttattcccgg gcttgcagga gcagatatca aaacactgct
     1981 tccgttcaat gtttataata tgtttacata cgtcctgcgc gaagaccagc cagtgctgaa
     2041 caaggatatc aaatacgacg acaggatgct caacatatca attttcccca tcaggaaaaa
     2101 caagatctgt ggagcaataa tacgtgacct ttactcgccc gaggtataca gcgaagaagt
     2161 tactgcccgt atttcggaag taattgagaa aaaccttgag atggtccaaa aaataggttt
     2221 tctgctcggt gaaggtgccg ccgaaacaga acagatgctc aactcaataa ttgaatttta
     2281 caaaaaggtt aaaacaaggt cggaaagcag ctaaaaacaa tttagatggc aaaggatttt
     2341 tttatcgaga taaacagcca gcagcgcaat cacgacggtc aaagaatatg cggtgatgtg
     2401 tttctttaca aatatgtgca ggaagaagac cgtgtaattg ctgtgctctc cgacggtatg
     2461 ggtcacgggg tgaaagccaa catgctggct acccttgcca caaccatggc gctcaacttt
     2521 acacgtgagc acaaggatgt caacaggata gctgagataa taatgaacac actccccgtc
     2581 tgcagccaga ggaagataag ctatgccacc ttcacaataa tagatattga aagcaatggc
     2641 agaatatcca tactcgaata tgataatccg cctgtaataa ttctccgggg cagcaattac
     2701 atagatattg actggtcgac gattaagctt gaaaaaggga aacatgccgg caagtcgctt
     2761 cgaaagtaca ccttcattcc aaaaaaagaa gaccgtatca taatttgttc cgatggcgtc
     2821 acgcagagcg gcatgggaag cgatacctac ccctttggct gggagcggga aaacgtcata
     2881 aaatatgcca caggccttat taaaggtgac cattcaatat cggctgacgc acttgcgaac
     2941 aaaattgtaa caatggctaa caaaaacgac aattacaggc cgcaggatga tattagctgt
     3001 gctgtcatat atttccgcga acctcgcaaa ctgcttcttt gcacaggtcc tccctacgaa
     3061 aaggagaaag acagggaact tgcaaagaag gttgaggagt tcaagggtaa agtgattgta
     3121 tgcgggggaa cgacagctga tattattgca cgcgagttga acagacccat taccgacaat
     3181 cttgtttttg aagaccccga actcccaccc gaaagcttta tacagggcat agacctcgtt
     3241 actgaaggaa ttcttaccct tcagaaagtg aacgaaatac ttaagacata caacaacagt
     3301 gtaagactcg ggaaaggacc tgccgacaaa attgtgaaac tgttcatgga aagcgatgaa
     3361 attcatttta ttataggcac tcgaatcaac atagcacatc aggatcccaa cctgcccgtt
     3421 gatcttgaaa taagaagaac tgttgtgaaa aggattgccc gtctcctgga agaaaaatgg
     3481 cttaagaaga caacttttga gtatatctga caacctggct gaacaggaca ggctgtataa
     3541 caggatatgt aagtgctgat ggcatatcag ctgaaaatat aacctcttca atttcttccc
     3601 tgtcgctgaa gttttccatc ttttttattt cggcaaagta taacttgcca tatcctgtaa
     3661 caccttgttc ttcaaccgaa tatgttgcaa cacaatgaat atcaaataaa actgctcccg
     3721 tctcttcagc aagttccctg caggcagcct cttccggtgt ctcaccctcc tccacatgcc
     3781 ctgccggcat ctcccatgtg tgttttcccc ggtgtctgac aaatacccac ctgcccctgt
     3841 aacggcatcc tattacagaa tacgt
//
LOCUS       DAOSNB010000254         3865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23652_length_3865_cov_3.675328, whole genome shotgun sequence.
ACCESSION   DAOSNB010000254 DAOSNB010000000
VERSION     DAOSNB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23652_length_3865_cov_3.675328"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..515)
                     /gene="pyrF"
                     /locus_tag="PLG42_09430"
                     /pseudo
     CDS             complement(150..515)
                     /gene="pyrF"
                     /locus_tag="PLG42_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
     gene            complement(529..1617)
                     /gene="prfA"
                     /locus_tag="PLG42_09435"
     CDS             complement(529..1617)
                     /gene="prfA"
                     /locus_tag="PLG42_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOU31469.1"
                     /translation="MAGNNIILERLEGVKQKFLEIGEMLTKEEVLADMKKYIKLNKEY
                     KELKPVVDAYQEYKLILDNIESAREMLATEKDEELRGMAKEELEKLEAKLPELEEKIK
                     ILLLPADPEDSKNAIVEIRAGTGGDEASLFAGDLFRMYTKFFESKGWKVEITRFTEGT
                     AGGYKEIVMRVIGEGVYGIMKYESGVHRVQRVPQTETQGRIHTSAASVVVLPEADEVD
                     VEIRNEDIRKDTYCSSGPGGQSVNTTYSAVRLTHIPTGIVVTCQDEKSQIKNYEKALK
                     ELRTRLYNLEYQKYLDEMAKKRKTMVSTGDRSAKIRTYNFPQGRMTDHRINYTVYNLS
                     SILDGNIQEVIDRLQMAENAERLKASNS"
     gene            complement(1749..2915)
                     /locus_tag="PLG42_09440"
     CDS             complement(1749..2915)
                     /locus_tag="PLG42_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOU31470.1"
                     /translation="MDLNSRYNKRGVSSSKEDVHKAISALDKGIFPNTFCKIVPDILG
                     RDPEYCNIMHADGAGTKSSLAYVYWKETGDMSVWKGIAQDAVVMNLDDLLCVGAYNDI
                     LLSSTIGRNKNLIPGEVISAIINGTEEFLEKLRNLGIGIWLTGGETADVGDIVRTIIV
                     DSTVAARMKRSDVVPVNIQPGDVIVGLASFGQTTYEDMYNGGMGSNGLTSARHDVLAK
                     YLSEKYPESYDPSIPEELVYSGMLKLTDTVEGLNVTAGKLLLSPTRTYAPVIKQVIEE
                     MHGEIHGMIHCTGGGQTKVLHFIDNLHIVKDNLFPVPPLFRIIKEQSDTSWEEMYRVF
                     NMGHRFEIYTNEKSAERIIEIASGFRLETRIVGYCGKSEKKKLTIKSEYGTFEY"
     gene            complement(2994..>3865)
                     /locus_tag="PLG42_09445"
     CDS             complement(2994..>3865)
                     /locus_tag="PLG42_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HOU31471.1"
                     /translation="YTMRRMFGRPPQGPGQGQGQTPPVPRVEFSFIGGPQLNIFRAPT
                     DNDIRSVGRATAALDLWNLKGEVTSFSFNKVNDRLVEVKIDMKSSIPTGYSIATNTTY
                     KVYGNGRVDISTVFIPDEANWSLPKLGFIFTLNEGFENVEYYGAGPFENYVDRKTAAF
                     IGRYRTTVDEMFVPYVRTQDCGNRTDVRWFTVTNHAGYGIMFTAPNLMNFSALHYTPL
                     DLDRANHPYELTRRKETILTVDMAGCGLGNGSCGPPPLERYLLKPTKAGFSYSIKPYA
                     PVLGDKTEVAKEY"
BASE COUNT          962 a          921 c          779 g         1203 t
ORIGIN      
        1 acgatagggc attacagcct gaatcaatga aaaaggttgc tccccgtgca gttattgcag
       61 acgatgctgc aggggcgctc aggaaggcaa aggcaatgtc aggcagaaat gatgtaatca
      121 tcgtgacagg ctctctatat ctaattggtg agtgtagagc tattattaat gatattttct
      181 aaaaaagctt tagcatacat gcgtgacgta ttgcctatat ctcccctttt ggcgtcggca
      241 ataacaaaga tgtcaggata gttgtccctt atgtatctga cagtcttttc caatgattcc
      301 cagcctctcg ctccattagc ctcgtaaaaa gcaacattgg gtttatatgc cacggcgtag
      361 ggatgcgttg tatcaattat tctccggttg aattcgaaaa ctggatcaga ttcagaaaaa
      421 accgaacgag gaagtttttc tatttccgga tcgagcccga cgcaaagaaa tgattttttc
      481 tttttaatgt tttcaaaaag ttcaaggctt gtcatcgctg ataaagtatt aagaattact
      541 ggctttcaac ctttcagcat tttcagccat ctgcagtctg tcaatcactt cctgaatgtt
      601 tccgtcgaga atggatgaaa ggttatatac agtatagttt attctgtggt cggtcattct
      661 gccctgcggg aagttatatg ttcttatttt cgccgaacgg tcaccggtag acaccattgt
      721 ctttcttttt ttcgccatct catcgagata tttctgatat tcaagattat agaggcgtgt
      781 tcgcagttcc ttaagggctt tctcatagtt ttttatctgc gacttttcat cctggcatgt
      841 aactacgatt cctgtaggta tatgagtcaa tcttacagct gaataggttg tgtttactga
      901 ctgtccacca gggcccgaag agcagtatgt gtcttttcta atgtcttcgt tcctgatttc
      961 cacatcaacc tcatcagctt cgggcagcac aaccactgaa gctgcagagg tatgaatgcg
     1021 tccctgtgtt tcggtttgtg gcaccctctg aacgcggtgc acacccgact catacttcat
     1081 aatgccataa acgccttcac cgatgactct catcactatc tccttgtatc ctccagcagt
     1141 accttcggta aaccgtgtaa tctcaacttt ccatcctttg ctctcaaaaa acttcgtgta
     1201 cattctgaag aggtcacccg caaatagcga tgcctcatca cccccagtac ccgctcttat
     1261 ctcaactatt gcattcttgc tgtcctcagg atcggcaggc aggagaagga tttttatttt
     1321 ctcctccagc tcaggaagtt ttgcttcaag cttctcaagt tcctccttcg ccattccccg
     1381 taactcctca tccttctctg tggcaagcat ctccctggca ctttcaatat tatcaagtat
     1441 cagtttgtac tcctgatatg catcaacaac cggcttaagc tctttatatt ccttattaag
     1501 cttaatatat ttcttcatat cggccagaac ttcctctttt gtcagcatct cgcctatttc
     1561 aaggaatttc tgtttcactc cttcaagcct ctcaagtatt atgttgttac cggccattgg
     1621 tttcaaaatt ttcaggctgc aaagataata aaaaggcaaa agttaaagct caatgttaaa
     1681 tataataaat agctctccgt tcagagcaac aacaatcagg tttaggcatt aaacatctgg
     1741 ctattagttt agtattcaaa agttccgtat tccgatttaa ttgtgagttt ctttttctcc
     1801 gattttccgc aataccctac tattcttgtt tcaagcctga agcctgaagc tatttcaatt
     1861 atccgttcag cggatttctc gtttgtgtat atttcgaatc tgtgtcccat attgaatact
     1921 ctgtacatct cttcccatga tgtatctgat tgttctttaa ttatcctgaa aagcgggggt
     1981 acaggaaaga gattgtcttt tacaatatga agattgtcaa tgaaatgtaa aactttagtc
     2041 tgaccaccac cggtgcaatg gatcatcccg tgaatttcac cgtgcatttc ttcaataacc
     2101 tgttttataa ccggagcata ggttcgggtt ggcgagagca ggagtttgcc tgccgttaca
     2161 ttaagtcctt caactgtatc tgtaagttta agcatgcctg aatacaccag ttcctccgga
     2221 attgaagggt catagctctc ggggtacttt tctgaaagat attttgcaag cacatcatgc
     2281 cgcgccgagg taagaccgtt gctgcccatc ccaccgttgt acatatcttc gtaagtggtc
     2341 tgaccgaatg acgccagccc cacaattaca tctcccggct gaatgtttac cggcacaaca
     2401 tcgcttctct tcatccgggc agcgacggtt gagtctacga tgatggttct gacaatgtcg
     2461 cccacatcgg ccgtttcccc gccagttaac cagattccta tgccaagatt gcgcaatttt
     2521 tccagaaatt cttcggtacc attaattata gctgatatta cttctcccgg aatcagattt
     2581 ttgtttctgc ctattgtgga agaaagcaaa atatcattat atgctcccac gcacagaagg
     2641 tcgtccaggt tcatcaccac agcgtcctgt gctatgcctt tccacaccga catgtcgccg
     2701 gtttctttcc agtacacata ggcaagcgat gatttcgttc ctgcgccgtc ggcatgcata
     2761 atattgcaat attccggatc tcttcccaga atgtcgggta caattttaca gaaggtgttg
     2821 gggaatatcc ccttatccag agcagagatt gctttgtgga catcttcttt tgatgatgag
     2881 acgcctcttt tgttatagcg tgaattcaaa tccatgtcgg aaatttcata atactaaaat
     2941 aaaaaaagta ttcaatccct catggattga atactttttc gcaaacatta tttttaatac
     3001 tcctttgcta cttcagtttt atcgccaagt acaggcgcat aaggcttaat tgaataactg
     3061 aaccctgctt ttgttggctt aagcaggtat ctttcgagtg gtggcgggcc gcagcttccg
     3121 ttaccaagcc cgcatccggc catgtcgacc gtaagtattg tctctttcct cctggttagc
     3181 tcatacgggt gatttgccct gtcgagatcg agcggggtat aatgcagtgc gctgaagttc
     3241 atcagattgg gtgcagtaaa catgatgccg taaccggcat ggttagtaac agtaaaccat
     3301 ctcacatcgg ttcggttgcc acaatcctgt gtcctaacat aaggcacaaa catctcgtca
     3361 acggttgtcc tgtaccttcc aatgaaagct gcagttttac ggtcaacata gttttcaaac
     3421 ggaccagcac cgtaatactc gacattctca aatccttcat tcagggtaaa gataaacccg
     3481 agtttcggaa gcgaccagtt ggcctcatcg ggaataaata cggtgctgat atccacccgg
     3541 ccgttaccgt aaactttgta ggtcgtattg gttgcaattg aatagcccgt tggaattgat
     3601 gacttcatgt cgattttcac ctcaacaagt ctgtcattga ctttattgaa gctgaacgat
     3661 gtcacttctc ccttcaggtt ccacagatcg agtgctgctg tggctctgcc taccgacctg
     3721 atgtcgttgt cggtgggtgc cctgaaaatg tttaactggg gccctccgat gaatgaaaat
     3781 tcaactcttg gtacaggtgg tgtctgaccc tgtccctgac ccggaccctg gggaggcctt
     3841 ccaaacatgc ggcgcatggt gtatc
//
LOCUS       DAOSNB010000255         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23658_length_3864_cov_4.857968, whole genome shotgun sequence.
ACCESSION   DAOSNB010000255 DAOSNB010000000
VERSION     DAOSNB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23658_length_3864_cov_4.857968"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..2215
                     /locus_tag="PLG42_09450"
     CDS             239..2215
                     /locus_tag="PLG42_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOU31472.1"
                     /translation="MKKRILILLLLILVASPAFSQKRRANKAYEFFRAGEYYEAIDQF
                     KNAYSKTRDRELKDEMIFMVAECYRLVNDPRNAELWYKRAVRSAYSRPEAQYWLADAL
                     KKNGKYQQAIEEFKKYKQLVPSDARADREIRACELADEWLRNPESYRVEELKDLNSRY
                     SDFSPAYGRDDFGVVYFTSSREGAQGKKTHGATGQSFADIFESRLDKKGKWSLPVPVE
                     SLNSEFEDGTPNLTPDYKEIYFTRCEAGKREKKGCVIMHSARKGEGWDDPKNIGLLAD
                     TLVAAHPAISPDGLTLYFVSDIPGGYGGKDIWYVTRDKPSGSWSGPKNAGPDINTPGD
                     EVFPYVRNDGVLFFSSDGHIGMGGLDIFKATPQPDGTWIVQNMRPPINSSADDFGIVF
                     QGNSESGIFSSTRKGRMNDELYYFERPPLRFSVTGLVKDEKTGAPIPAATVQLIASDG
                     ANLQTETGTGGDFKFTLRPNVDYILLASKKNYLNGKERVTTKGQEKSRDFMVTILLSS
                     IERPIELPNIYYDFDRWELRPESMVSLDKLVETLNDNPNVTIELMSHTDSRASDEYNL
                     ELSQKRAQSVVDYLVSKGIDIERLSAKGYGESMPKVVDADLAAQYPFLKQGVTLTEQY
                     INSLANDEQREIAHQINRRTEFRVLRTDYEPKKK"
     gene            2237..2959
                     /locus_tag="PLG42_09455"
     CDS             2237..2959
                     /locus_tag="PLG42_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOU31473.1"
                     /translation="MKKIFFSFLVSFALLSTFAAKPPVKILVVTGGHGYNKETFNEML
                     NSLGSEFTYEVAELPDAFRMFLPENRNKYDVLLFYHMWQTITPEQKEAFVSCIKDGKP
                     VVALHHSICAFDDWEEYWRIIGGKYFHKPTLLDGKEYPACSYIHDLHFNVKVADKKHP
                     VTKGIKDFEIFDETYKGYWVDPSVKVLLTTDEPSSTPVIGWAKTYGKAKIVVLQSGHD
                     TPTFQQAEFRQLLRQALEWVVK"
     gene            complement(3068..>3864)
                     /locus_tag="PLG42_09460"
     CDS             complement(3068..>3864)
                     /locus_tag="PLG42_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31474.1"
                     /translation="GVQVPGVMMITWPDLYYHTSQDVADKCDPTQLKRVCFIGAAAAY
                     TVASAGEETAMKIAADVAGNSQARIGKQLQRAIDQLDKAKSDNFENLYKLTKSYIEAA
                     IINEKATIETVQELAPSSATLKAGIAKLIASVDAAGKATLAIFDSYAGTKALSLGIAS
                     TSFKPTETETRAKTIVPKPTPLVTQNGYRGYSQIISKLDPSIREKYPIKGRFLDAQEL
                     CRLCNGRNSALDIKKLLDAQMKSGEVELQDVLNYIYILKEAGLVTL"
BASE COUNT         1153 a          794 c          845 g         1072 t
ORIGIN      
        1 acaagctcga aagaatagta gataacgaaa aatggccatt gccaaagtac agggagatgc
       61 tctttacacg ttaacagctg aaggaaaatc ttcaagaatc agatttaaca gttaatttta
      121 aaaaagaggc ttttgaaaca gcctcttttt tattataaat ggtcaattta ttattataat
      181 ttaaggcttt ctgatacgtt tgtacaataa acatcactat ttttaccgca gggttattat
      241 gaaaaaacgt atcttaatat tattactgct gattctggtt gcttcacctg cttttagtca
      301 gaagcgaagg gcaaacaagg cttatgagtt tttcagggcg ggagaatatt atgaggcaat
      361 agaccagttt aaaaatgcct attccaaaac aagagacagg gaacttaagg atgaaatgat
      421 ttttatggtt gcggaatgtt accggcttgt gaatgacccg cgtaatgcag aattgtggta
      481 taaaagggca gtgcgatcag catattccag accggaagca caatactggc ttgctgatgc
      541 tttgaaaaag aatgggaaat accagcaagc tatcgaggaa tttaaaaaat acaaacaact
      601 tgttccgtca gatgcaaggg ccgacagaga aatccgtgcc tgtgaacttg ctgatgaatg
      661 gctcaggaat cctgaatcgt acagggttga agagttgaaa gatctcaatt caaggtactc
      721 tgatttcagt ccggcatatg gcagggatga ttttggagtt gtttatttta cttcatcaag
      781 agaaggggca cagggaaaga aaactcatgg tgcaacaggc cagagttttg ctgatatttt
      841 tgaatcacgg cttgataaaa agggaaaatg gagcctgccg gtaccggtgg aatcacttaa
      901 cagtgaattt gaagacggaa ctcctaacct gacacctgac tacaaagaaa tatattttac
      961 cagatgtgag gcaggaaaaa gggaaaagaa aggttgtgta ataatgcact cagccaggaa
     1021 aggtgaaggc tgggacgatc cgaaaaatat cggtcttctt gctgatactc tagtagcagc
     1081 tcatcctgca atatcacccg acggacttac gctctatttt gtttccgata tccccggtgg
     1141 ctatggggga aaagatatat ggtatgtgac acgagacaaa ccatccggaa gctggtcggg
     1201 tccgaaaaat gcaggtcccg atattaatac tcccggagat gaggtctttc catatgtaag
     1261 aaatgacggc gtgcttttct tttcttccga tggtcatata ggtatgggag gtcttgatat
     1321 cttcaaagca acccctcaac ccgacggcac atggatcgtt caaaacatga gacctccaat
     1381 taacagcagt gccgatgatt tcggaatcgt tttccagggc aactccgaga gcggcatctt
     1441 cagctccaca agaaaaggaa gaatgaatga tgagctttat tatttcgaac gcccacctct
     1501 caggttctca gtaaccggat tggtaaagga tgaaaaaaca ggcgcaccaa tacccgctgc
     1561 aactgttcag cttattgcaa gcgacggcgc aaacctccag acagaaaccg gtacaggcgg
     1621 agactttaaa tttacactgc gcccgaatgt ggattatatc ttacttgcat caaagaaaaa
     1681 ttacctgaat ggaaaggaaa gagtgacaac aaaaggacag gaaaagagcc gcgactttat
     1741 ggtaacaatt ttgctttcat ccattgagcg tcctatcgaa ctgccaaaca tctactacga
     1801 tttcgacaga tgggaactga gaccagagtc aatggtttca ctcgataaac ttgttgaaac
     1861 tctaaatgat aatcctaatg taacaattga actgatgtct cataccgact cgagagcaag
     1921 cgatgagtat aaccttgaac tttcacagaa aagagcccag tcggttgttg attatcttgt
     1981 ttcgaaaggc attgacatag aacgactctc agcaaaagga tatggcgaat caatgcctaa
     2041 agtggtagat gccgatctgg ctgcccagta tccgttcctg aaacagggtg ttacacttac
     2101 tgaacaatat atcaacagtc tggctaatga cgaacagcgc gaaatagcac accagattaa
     2161 cagaagaaca gaattcaggg tgcttagaac agattatgag cctaaaaaga aatgagtgtt
     2221 acaaaatatc cttcttatga aaaaaatatt cttttcattt cttgtttcat ttgccctgct
     2281 ttctactttt gctgctaaac cgccagttaa aatacttgtt gttacaggcg gacacggata
     2341 taataaggaa acattcaatg agatgcttaa cagccttgga agcgaattca cttatgaggt
     2401 ggctgaatta cctgatgctt tcaggatgtt ccttccagag aataggaaca aatatgatgt
     2461 gctgcttttt taccatatgt ggcagacaat aactccggaa cagaaagagg cttttgtgtc
     2521 atgcattaaa gatggcaagc cggtagtggc acttcatcac agtatctgcg ccttcgacga
     2581 ctgggaagaa tactggagaa taataggcgg aaaatatttt cacaaaccaa ctctccttga
     2641 cgggaaggaa tatccggcat gttcatatat acatgatctt cattttaatg taaaagtggc
     2701 tgataaaaaa catccagtaa ctaaaggaat aaaggatttt gaaatatttg acgaaactta
     2761 taaaggttac tgggttgacc catcagttaa ggtgcttctc acaaccgatg aaccatcgag
     2821 tactccggtt ataggctggg caaaaacata cggaaaggca aagattgttg tgcttcagtc
     2881 aggtcatgac acccctactt ttcagcaggc tgaattccgg caacttctca gacaggcact
     2941 ggaatgggta gtaaaataaa catttaacac tctcatccct catttctcat ccctcatttc
     3001 tcatcgctca ttcctcattc ctcattcctc attcctcatt cctcatcctt tccccccatg
     3061 ctgcatgtta taatgtaaca agacctgctt ctttaagaat gtagatataa tttaaaacat
     3121 cctgaagctc cacctcacct gacttcatct gggcatcgag cagtttcttt atatccagag
     3181 ctgagttcct tccgttgcac aacctgcata gttcctgtgc atcgagaaag cggcctttaa
     3241 tcgggtattt ttctcttatt gaaggatcga gctttgagat tatctggcta tatcccctgt
     3301 aaccgttttg cgtaacaagg ggagtgggtt ttggaacaat tgtttttgcc ctggtttctg
     3361 tttcggttgg cttgaaggat gttgaagcta ttccgagaga tagtgccttt gtcccggcat
     3421 aactgtcaaa tattgcaaga gtggcttttc ctgcagcatc aactgaagca atgagttttg
     3481 ctatgccagc cttcagtgtt gccgaggaag gcgcaagctc ctgaacggtt tcaatcgtag
     3541 ccttttcgtt gattattgcc gcttcgatgt agctttttgt caatttgtac agattttcaa
     3601 agttgtcact tttagccttg tcgagttgat caattgcccg ttgcagctgt tttccgattc
     3661 ttgcctgact gtttcctgct acatcggcag caatcttcat ggctgtttct tcgccggcac
     3721 tggcgacagt atatgcagca gcagcgccta tgaaacagac tcttttaagc tgtgtggggt
     3781 cgcatttgtc ggcaacatcc tgtgaggtat ggtaatagag gtccggccat gttatcatca
     3841 ttacacccgg aacctgaacc cccc
//
LOCUS       DAOSNB010000256         3843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23819_length_3843_cov_3.097149, whole genome shotgun sequence.
ACCESSION   DAOSNB010000256 DAOSNB010000000
VERSION     DAOSNB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23819_length_3843_cov_3.097149"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLG42_09465"
     CDS             <1..1083
                     /locus_tag="PLG42_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOU31475.1"
                     /translation="QCGDCSTVCPHADEVYRFPRKSMKLLQMGLEQKIETTLEPWLCY
                     YCGQCSEQCPREADPGETMMSLRRWLISKYDFTGISRAFYKSRTVEVLTIIFVAILTG
                     ALLLYYGFTNGNIGIYDGEGAFLPSHFVHRFDLSVGAILLVFLLINAFRMWKMVMVRG
                     TNFPVPWWLYIKKFYQLPWHFFTQKRYSECEKKRGHKIYMPWLIHLGLMLGYVTMLVL
                     VMVFVELLQKGPEIRWEVHVFGYLATIGLLAGTIYFIRGRFKKHYIQYKKSHGTDWVF
                     VILLFMIVITGISQHIFHRTGLIEMANITYVIHLMSAVPWLLRMPFTKWAHLVYRPLA
                     MYFASIRREAIERDTAVKELYPLVIK"
     gene            1092..2831
                     /locus_tag="PLG42_09470"
     CDS             1092..2831
                     /locus_tag="PLG42_09470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOU31476.1"
                     /translation="MENLRIGVYICMCGTNIAKIVDVEAVAKFASEVPEVVLAKSYKY
                     MCSNPGQEMIIQDIKENNLNRIVVAACSPNMHERTFRSALRIAGLNQYLLEMANIREQ
                     CSWVHSDPVEATEKAKALVNAAISRVKLHEELESVSARMCPNVLVIGGGIAGMTAALE
                     LADAGNTVYLVEKENHLGGNLARVDLTAPYLYSARDLLTERITRINNNKNIKVFLSSK
                     VTSLTGYIGNFRAVIQNLVSIQEGKTRDTEVEIGNVIVCTGYKEFDASRIVHYGYGKL
                     PNVITSFEFEKMLRAGRIETKEGKTPKYVAIIHCVGSRSQEFHSYCSRVCCMTALKYA
                     HEIKSAIPDCYVSDIYIDMHAFGKGHEDFYRSSSQAKTLFLMYEKGDRPVIHKAGPKD
                     DCGMLIEVNEKLSGETIEIPADLVILMVGMEAREDAYEVARIVNISQDKEGWFIESHP
                     KLEPVATTTDGVFIAGTCVAPKDIPDTVAQARAAAARILARIAKEKIEIDALYSEVNP
                     NLCSGCRLCNLLCPYSAIEFLEDKKQSHIISALCKGCGVCVAACPSSAIKGRHFTDQQ
                     ILAQVEGLLAEKL"
     gene            2864..>3843
                     /locus_tag="PLG42_09475"
     CDS             2864..>3843
                     /locus_tag="PLG42_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31477.1"
                     /translation="MEEKLVPAHIFFLRWFIRIRWIAVSALIASDFIVPHFLNISVEE
                     DKIYIIAGILFLLNVFHWIVLRRINRDSGANVIGRIKRNIHFQIITDLILLTFVIHYS
                     GGIENPLILFYFFHLIIASSIFSTFLSYLYAAFALILALSLSFLECFQIIPHYPLEGF
                     VNHELYKNVLYVSGAGFVFACTSVMVVWLSHMIINRSIKSEETYVRTNIELQNKDKLK
                     NEYVLRVTHDIKGHVAAVMSCLEVVRSKITGSLNEVQEEFINRAYDRAEFLSEFIRDL
                     LNLTKKRLQRNVEFEDFNLAELINRVVAPVQILISDKGINFNIYVDSSVG"
BASE COUNT         1144 a          698 c          856 g         1145 t
ORIGIN      
        1 cagtgcggag attgctccac tgtctgtcct catgcagatg aggtgtacag gtttcccaga
       61 aaatctatga aactgcttca gatgggcctt gagcagaaaa tagaaactac tctggaacca
      121 tggctttgct actattgcgg gcagtgttct gaacaatgtc cgagggaagc tgatcccggt
      181 gaaacgatga tgagcctcag gagatggctg atctcaaaat atgattttac cggtatttca
      241 agggctttct ataaatcgag aactgttgag gttttaacaa ttattttcgt ggcaatactc
      301 accggagccc ttctgctcta ttacggattt acaaacggta atatcggtat ctacgacggt
      361 gaaggagcat ttcttccaag ccattttgtt cacaggtttg accttagcgt aggtgcaatc
      421 ttgctcgttt ttcttcttat taatgctttc aggatgtgga aaatggttat ggtcagggga
      481 acaaattttc cggtaccatg gtggttgtat attaaaaagt tttaccagct tccgtggcat
      541 tttttcactc agaaacgcta ttcggagtgt gaaaaaaaac gtggtcataa aatctatatg
      601 ccgtggctga ttcacctcgg actgatgctc ggatatgtta caatgcttgt gctggtgatg
      661 gttttcgttg aattgcttca aaagggtccg gaaataagat gggaagtaca tgtctttggt
      721 tatctggcca caataggact attggcggga actatctatt tcatcagagg acgttttaaa
      781 aaacattata ttcagtataa aaaatcacat ggtactgact gggtttttgt gattctcctg
      841 tttatgattg ttataaccgg aatttcacag catatttttc accgtaccgg tttgattgaa
      901 atggccaata taacttatgt tattcacctg atgagtgctg tgccatggct tttaagaatg
      961 ccgtttacaa aatgggctca tcttgtttac cggcctcttg caatgtattt tgcatcaatc
     1021 agacgtgaag caattgaaag ggatacagca gttaaagaat tatatcctct tgtaattaaa
     1081 taacctttac tatggaaaat ctcagaattg gtgtttatat atgcatgtgc gggaccaata
     1141 ttgcaaagat tgttgatgtt gaagctgttg caaaatttgc atcagaagtt cccgaagtgg
     1201 ttctggcaaa aagttataaa tacatgtgtt ccaatccggg tcaggaaatg ataattcagg
     1261 atattaaaga aaataacctg aacaggattg ttgttgcagc ctgcagcccc aatatgcacg
     1321 agcgcacatt ccggagcgca ttgcgtatag cagggctgaa ccagtatctg cttgaaatgg
     1381 caaatatcag ggaacagtgt agctgggttc actccgatcc tgttgaggcc accgaaaaag
     1441 caaaagcact tgttaatgca gccatctccc gtgttaaact tcatgaggaa ctggagagtg
     1501 tatccgcccg gatgtgcccc aatgttcttg ttataggcgg aggaatagca gggatgactg
     1561 ctgcacttga acttgcagat gcaggaaata ccgtttatct ggttgaaaaa gaaaatcacc
     1621 ttggaggcaa ccttgccagg gtcgacctta cagcacctta tctctattcg gcaagagatt
     1681 tgttgactga aagaataaca aggattaaca acaataaaaa cataaaggta ttcttaagtt
     1741 ccaaagtaac aagcctcacc ggatacatcg gaaacttcag agccgtaatt caaaaccttg
     1801 tttctattca ggagggtaaa acaagagata ctgaagttga aatcggtaat gtaattgtat
     1861 gcacgggata caaggaattt gatgcttcac gcattgtaca ctacggatac ggaaaactgc
     1921 caaacgtcat cacttcattt gaatttgaaa aaatgcttcg ggcaggcagg atcgagacaa
     1981 aggaaggaaa aactccgaag tatgtagcaa ttattcactg tgtaggaagc agaagtcagg
     2041 aatttcattc atattgttca agagtatgct gtatgacagc actaaaatat gctcatgaaa
     2101 tcaaatcagc aattcccgac tgctatgttt ctgatatata catagatatg catgcttttg
     2161 gcaaagggca tgaagatttt taccgcagtt cctcacaggc aaaaactctt ttcctgatgt
     2221 acgaaaaggg tgaccgacct gtaattcaca aggcagggcc aaaggatgat tgtgggatgc
     2281 tgattgaagt gaatgaaaaa ctttcgggcg aaacaattga aattcctgca gacctcgtca
     2341 tacttatggt ggggatggaa gcaagggaag atgcctatga agtggcaagg atagtgaata
     2401 taagtcagga taaagaagga tggttcattg aaagccatcc aaagcttgaa cctgttgcta
     2461 ccacaactga tggtgttttt attgccggta catgtgtggc tcctaaggat attccggata
     2521 ctgttgctca ggcaagggca gctgctgcaa gaatccttgc acgaatagcg aaagaaaaaa
     2581 ttgaaattga tgcactctat tcagaagtga atccaaatct gtgttcggga tgtcgcctct
     2641 gcaaccttct ctgtccttac agcgctatag agtttcttga agataaaaaa cagagtcata
     2701 ttatcagtgc tctctgcaag ggatgcggag tgtgtgtagc agcctgcccc tcatcagcaa
     2761 ttaaaggaag acatttcacc gaccagcaga ttctcgctca ggttgaagga ttactggctg
     2821 agaaactgta aattcggaat tattatactg acagtatatt tttatggaag aaaagctggt
     2881 tccggcacat atattcttcc tgagatggtt tatcagaatc aggtggatag ctgtttcagc
     2941 attgattgcc tcagatttta ttgttccaca ttttctgaac atatctgtcg aggaagataa
     3001 aatctatatt atcgccggaa ttcttttttt acttaacgtt tttcactgga ttgttctcag
     3061 gagaataaac agagattcgg gtgccaatgt tataggaagg ataaaaagaa atatccattt
     3121 tcagataata actgatctga tcctgcttac attcgttata cattattcgg ggggaataga
     3181 aaatcccctg atattattct atttttttca tcttattatt gcaagttcaa tattctcgac
     3241 ctttctgagc tacctgtatg ctgcctttgc tttaatactg gctttgtcgc tgagttttct
     3301 ggaatgcttt cagattatcc cccattatcc tctggaggga tttgttaatc acgaacttta
     3361 taaaaatgtt ctgtacgtct caggtgcagg ttttgtattt gcatgtacat ctgttatggt
     3421 tgtatggctc tctcatatga ttattaaccg gtcgataaaa agtgaagaga catacgttag
     3481 aacgaatatt gagctacaga acaaagacaa gcttaaaaac gaatatgttc tgagggttac
     3541 acatgacatt aaaggacatg ttgctgctgt catgagttgt cttgaagttg tcaggtcaaa
     3601 aataacaggg tcgcttaacg aggttcagga agaatttatt aaccgggctt atgatagggc
     3661 ggaatttctt tcggaattca taagagattt actcaacctg acgaaaaaaa gacttcaacg
     3721 taacgttgaa tttgaggatt tcaaccttgc agaacttatc aaccgggtag ttgctcctgt
     3781 tcagattctt atcagcgaca aaggaattaa tttcaatata tatgttgaca gttcggtcgg
     3841 gat
//
LOCUS       DAOSNB010000257         3835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_23865_length_3835_cov_4.504497, whole genome shotgun sequence.
ACCESSION   DAOSNB010000257 DAOSNB010000000
VERSION     DAOSNB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23865_length_3835_cov_4.504497"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLG42_09480"
     CDS             complement(<1..186)
                     /locus_tag="PLG42_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HOU31478.1"
                     /translation="MKKLLTLLIAVVVPGILIAGGLVTNTNQSAAWVRMPSRNASTSI
                     DAVNYNPAGLMKLENGFH"
     gene            complement(377..3514)
                     /locus_tag="PLG42_09485"
     CDS             complement(377..3514)
                     /locus_tag="PLG42_09485"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31479.1"
                     /translation="MKKKLTDNFLFTGLFSLILLLGYSCGPKKAEITGVNDFKSLSAN
                     FKEPPAEYTTAPFFVWNGEITQAEIDNFMDEFYRQGIKQVFVHPRPGLITEYLSDEWF
                     ELFRYTVDRGKELGMKVWIYDENSYPSGFAGGHVPANMPESYNQGQGLRMTRVETLPD
                     TADKFFIILKEENGVFTDITSSYQTEMGKTGKYCLFRKTFHSPSEWYGGFSYVDLLYP
                     GVTQKFIELTMQGYEKIAGEEFGKTIPGTFTDEPHIHTSGGIRYTPDLFDVFLKMWNY
                     DLKTALPSLFEQTGDWKKVRHNYTCTLLQMFIDRWAKPWKEYCDSAGLKFTGHYWEHE
                     WPKMHHGGDNMAMYAWHQVPGIDMLFNQWNDTSSTAQFGNVRSVKELASAANQAGWNR
                     KLSETYGGSGWDLTFTEMKRNGDWEYALGVNLMNQHLTFFTMAGARKYDYPPTFDYHE
                     PWWNNYKLLADHYARLSLALSSGRQINEILVIEPTTTTWMYDSYLKPDPYRDVIGQTF
                     QKFITKLEKQQVEYDLGSENIIKDLGSVKKGKFVVGNCAYKTVVIPPLTENLNLKTFT
                     LLRQFVKEGGKLLLFSSPSLVDGSENEELKEFISSSSNNVVKCESLNDELIKTHLGNR
                     DVTFTSIEGEGLFHHTRVLKDGLVVFLANSSLETSAAGTLTAPGVTAVELNTIDGEIK
                     GYPAEKKDKKVEARFSLAPAGSLLLFFPSQGISIPDVVPENVNTEKVIESEEKVTVTR
                     DEPNALMIDFCDLELEGKTEADMHIYDAADKVFKHYGFASGNPWNHSVQYKKAIVERD
                     TFGSGTGFRVTYKFIIKNLQDKSTLLAVVERPELWKVTLNGTEISPNLDKWWLDRSFK
                     VFNIANIVKEGENRLTLSVTPMKVHAEIEPVYILGDFSVEPARKGWVITSPMKELTTG
                     PWVKQGMPFYSWGVTYSKNYTIESPGGKYILKVTDWKGTVSEVFVNGKKAGIIAFPPY
                     EADITPFISAGKNRIELKVIGSLKNLLGPHHNNPPAGIASPWSWRNVRSYPPGSEYNL
                     KEYGLSGDFNIVNRN"
     gene            complement(3533..>3835)
                     /locus_tag="PLG42_09490"
     CDS             complement(3533..>3835)
                     /locus_tag="PLG42_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan N-sulfatase"
                     /protein_id="HOU31480.1"
                     /translation="LSFGQRGAEELYRIGIDPDCVDNLINNPGYSSAWQKMRETMFEL
                     LSEQQDPRILGKGDIFDRYTYADEKVRDFYNRFMKGELNTKSAGWVDSTDFEIKRK"
BASE COUNT          997 a          830 c          779 g         1229 t
ORIGIN      
        1 gtgaaaaccg ttttcaagtt tcatgagtcc tgcggggttg taatttactg catcgatgga
       61 tgttgaagca tttctcgacg gcatacgtac ccatgcagca ctctggttgg tattggtcac
      121 aagaccaccg gcaataagta taccgggtac caccacagca attaaaagcg taagcagttt
      181 tttcatagat taaattttta gtttatcggt tattttcagt tcaccaacct gttcattata
      241 tccgcaaact tcaataattt caggttgccg gtaattgaac aggcgccgta aatgtagaaa
      301 aatattttgt ttctgcaata gtcgaattat aaaatattaa ttaacagaac attaaaatta
      361 aaataattta tccggtctaa tttctattta caatattgaa atcacctgat aatccgtact
      421 ctttaaggtt gtattcactg cccgggggat atgacctgac gtttctccag ctccatggcg
      481 aggcaatacc tgccggggga ttattatgat gaggcccgag aagattcttc agactgccga
      541 ttaccttcaa ctctattctg ttttttcccg cagatatgaa cggagtaatg tcagcctcat
      601 aaggaggaaa ggcaatgata cctgcttttt taccattgac aaaaacttct gaaacagttc
      661 ctttccaatc agtcaccttt aaaatgtatt tgcctccggg tgattcaatg gtgtaatttt
      721 tcgaataagt tacgccccac gaataaaacg gcataccctg tttaacccaa ggaccagtag
      781 taagttcttt cattggtgat gttatcaccc atcctttcct ggcaggttca acagaaaaat
      841 ctccaagaat atatacaggt tctatctcgg cgtgaacttt cataggtgta acagaaagtg
      901 taagccggtt ttccccctcc ttaacaatat tggctatatt aaagacttta aatgaacggt
      961 cgagccacca tttgtcgagg ttggggctaa tttcggttcc gttcagagta accttccata
     1021 attccggtct ttccaccaca gcaagcagcg ttgacttatc ctgcaggttt tttatgatga
     1081 atttgtatgt aactctgaaa cctgtaccgc tgccgaatgt gtctctttcg acaatggctt
     1141 ttttgtactg cacagaatga ttccacgggt tgcccgatgc aaatccgtaa tgcttgaata
     1201 ctttatcagc tgcatcatat atatgcatat cagcctctgt tttgccttcc agttccagat
     1261 cgcagaaatc aatcatcagt gcatttggtt catctcttgt aactgtcact ttttcttcag
     1321 attcaataac tttttccgta ttcacatttt ctggaacaac atcgggaatt gaaattccct
     1381 gcgatgggaa gaacagcaga agactgcctg caggggccag actgaaacgt gcttcaactt
     1441 ttttatcttt tttctctgca gggtaacctt ttatctctcc gtcaattgta ttgagttcaa
     1501 cagcagtaac tccgggtgct gtaagggttc ccgcggcgct agtttcaagg cttgaatttg
     1561 caaggaaaac cactaaacca tctttcagca cgcgtgtatg atgaaacaat ccttctcctt
     1621 cgatagatgt gaacgtaaca tccctgtttc caaggtgggt ttttatcaat tcgtcgttaa
     1681 gtgattcaca tttaaccaca ttgttggatg acgaacttat aaactctttc agttcttcat
     1741 tttcggagcc atcaacaagc gaaggagatg agaaaagcag tagtttacca ccctctttaa
     1801 caaactgccg gagaagcgta aatgtcttaa gattaagatt ttctgtaagt ggcggtatga
     1861 caacagtttt atatgcacaa ttcccgacga caaattttcc ctttttcacc gatccaaggt
     1921 cttttataat gttttcagaa ccaaggtcat attccacctg ttgtttttcg agttttgtaa
     1981 taaacttctg aaatgtttgt cctattacat cacggtaagg gtcgggtttt agatatgaat
     2041 catacatcca tgtagttgta gtcggttcaa ttacaagtat ctcattgatt tgtctgccgg
     2101 aggagagggc aagggagagt cttgcgtaat ggtcggccag gagtttgtag ttgttccacc
     2161 atggttcatg gtagtcgaat gttggcgggt agtcatattt tcttgcacct gccattgtga
     2221 agaatgtgag gtgctggttc atcaggttca cgcccagggc atactcccag tcgccattgc
     2281 gtttcatctc agtgaatgtg aggtcccagc ctgagccacc gtatgtttcg ctgagctttc
     2341 tgttccaccc tgcctggttg gcagcgcttg caagttcttt cactgagcgt acatttccga
     2401 attgagccgt tgaagaggtg tcgttccact ggttgaagag catatctatt ccaggaacct
     2461 gatgccatgc gtacatcgcc atattgtcac ctccatgatg cattttgggc cattcatgtt
     2521 cccagtagtg acctgtaaat ttcaatcctg cactgtcgca gtattctttc catggctttg
     2581 cccagcggtc gataaacatt tgaagcaaag tgcaggtata attatgcctt accttcttcc
     2641 agtctccagt ttgttcaaag agtgatggca gggctgtttt aaggtcatag ttccacattt
     2701 taaggaatac atcaaaaagg tcgggggtat aacgaatccc gccagatgta tggatatgtg
     2761 gttcatcggt gaaggttcct ggaatagttt tgccaaactc ttctcctgca attttttcgt
     2821 aaccctgcat tgtaagttca ataaatttct gagttacacc cggatagagg aggtcaacat
     2881 atgaaaagcc accgtaccat tccgatgggc tatggaatgt ttttctgaaa agacaatatt
     2941 tgcctgtttt gcccatctct gtttgataag agctggtgat atctgtgaac actccatttt
     3001 cttctttcag aataataaag aatttatcgg cagtgtcggg cagtgtttca acacgggtca
     3061 ttctgagacc ctgtccctgg ttgtacgatt cgggcatatt tgccgggacg tgtcctccgg
     3121 caaaaccgct tggatatgag ttttcatcat atatccacac tttcataccg agttcctttc
     3181 ccctgtcaac cgtatatctg aacaattcaa accattcatc cgagaggtat tctgtaataa
     3241 gtccgggccg gggatgaaca aagacctgct ttatgccctg gcggtagaat tcgtccatga
     3301 aattgtcaat ctctgcttgc gttatctcac cgttccagac aaagaatggg gcggtggtgt
     3361 attctgcagg cggctctttg aaattagcag aaagagattt gaaatcattt acacctgtaa
     3421 tctctgcttt ttttggtcca cacgaataac ccagaagcag aattaaagaa aataatccgg
     3481 tgaataaaaa attatcagtt agttttttct tcataattaa aattttagtt cattattttc
     3541 tttttatttc gaaatcagtt gaatccaccc atccagctga ttttgtattt agttcacctt
     3601 tcatgaatct gttgtagaag tcacgcactt tttcatcggc ataggtatac ctgtcgaaaa
     3661 tatcaccttt ccccagtatt ctaggatctt gttgttcgga aagcagctcg aacattgttt
     3721 ctctcatttt ctgccatgcg gagctgtagc cggggttatt tatcaggttg tcaacgcaat
     3781 cgggatctat tccgattctg tacaattctt ccgctcctct ttgcccgaac gacag
//
LOCUS       DAOSNB010000258         3817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24025_length_3817_cov_3.479001, whole genome shotgun sequence.
ACCESSION   DAOSNB010000258 DAOSNB010000000
VERSION     DAOSNB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24025_length_3817_cov_3.479001"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLG42_09495"
     CDS             <1..938
                     /locus_tag="PLG42_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOU31481.1"
                     /translation="TIMEEGTELLDRLTRYLKGDTDARLPILTSCCPGWVNFFEYYYP
                     DLRDVPSTAKSPQQMFGAIAKTYFADKIGVDRKNLVVVSIMPCLAKKYECQRDEFSVD
                     GNPDVDYSISTRELAAFIRQANIDFNNLPEEEFDLPLGESTGAGVIFGTTGGVIEAAV
                     RTAYELYTGKKLGRIDFTELRGFEGVRSATVDFGELKLNIGIAHGLGNARKLLDDVRS
                     GKSKFHAIEIMACPGGCIGGGGQPLHHGNSEILKARAAAIYQEDRNKPLRKSHENPVI
                     IKLYEEFLGKPNSEKAHKLLHTHYFERKKKIIVKP"
     gene            966..1448
                     /gene="nuoE"
                     /locus_tag="PLG42_09500"
     CDS             966..1448
                     /gene="nuoE"
                     /locus_tag="PLG42_09500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOU31482.1"
                     /translation="MSSIKIELRKEDVEKIKEICASFNNDPQELINVLHKCQEHFGYL
                     PAEVQEVVSDCLNVPVAKIYGVVTFYSFFTMTPKGKYPISICMGTACYVRGAEKVLEE
                     FKKELGIQVGQTTPDGKFSLSSLRCVGACGLAPVVLVGEKTYGRVAPDDVKNILKEYE
                     "
     gene            complement(1582..3366)
                     /locus_tag="PLG42_09505"
     CDS             complement(1582..3366)
                     /locus_tag="PLG42_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOU31483.1"
                     /translation="MLSEKYKWLPTGADEMKALGWDQADVIIFTGDAYVDHPSFGAAI
                     IARVIEAEGMRVAIVPQPNWKDDLRDFKKLGPPVYLFGVTAGNMDSMVNHYTAFRRLR
                     SDDAYTPGGKAGFRPDYPTIVYTSILKSLYPDVPVVIGGIEASMRRLTHYDYWKDSIE
                     PSILISSGADMLVYGMGEQPIIEIVRLLKKGVPFSSLKTVPQTVIKLPADEPLPARKD
                     WKDRFLNSYEKCVADKNAFADNFIVFERESNKVNAARLIEPCGKYNVIVNPPFPPFNE
                     TEASRIYSLPFTYQPHPRYRKKEPIPAYEMIKFSVNIHRGCFGGCSFCTISAHQGKFV
                     INRTEESIMQEIDRISALPSFRGYLSDLGGPSANMYGMKGKNEELCRKCSRPSCIWPS
                     VCDNLNTSHERLLKLYRMVNSRKDIKKAFVGSGVRYDLLFKEWNPQAGKAEKEYLKEL
                     IINHVSGRLKVAPEHTEDHIVGLMRKSPFSLFIKLKKIFDETVKENGLNYELVPYFIS
                     SHPGCTIDDMQRLVNKIEPLGLRPEQVQDFTSTPMTLSTMMYYLGKDPYTGRKIFVER
                     DIEGKRRQKNMFFTKYKGHGLNRKFKKK"
     gene            3517..>3817
                     /locus_tag="PLG42_09510"
     CDS             3517..>3817
                     /locus_tag="PLG42_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31484.1"
                     /translation="MDIRRITDQHKAVCRLVADKKVKNSLEVLNSMISVSGRGQFRDE
                     YENLAMTYRNILQYTIEGVKDPERDKIYNILLRSIINLADRVKQDILSYYSGWYAY"
BASE COUNT         1154 a          800 c          792 g         1071 t
ORIGIN      
        1 ttactattat ggaagaaggt accgaactgc tcgacaggct cacccgctac ctgaagggtg
       61 acacagatgc aaggcttccc atcttaacct catgctgccc gggatgggtg aatttctttg
      121 aatattacta ccccgacctg cgggatgtac cttcaactgc aaaatcaccc caacaaatgt
      181 ttggcgctat tgccaaaacc tattttgcag ataaaatcgg ggttgacagg aagaatctgg
      241 ttgtagtatc aataatgcct tgtcttgcaa agaaatacga gtgccagaga gatgaatttt
      301 cagtcgacgg aaaccctgat gttgattatt caatctcaac aagggaactg gcagccttca
      361 tcaggcaggc aaacattgat ttcaataatc ttccagagga agagtttgac ctgccacttg
      421 gtgaatcaac cggtgcagga gtcatcttcg gcacaacagg cggtgtaatc gaagctgctg
      481 taagaacagc ttatgagctt tacacaggca aaaaattggg caggatcgac ttcaccgaac
      541 ttagaggttt tgaaggtgtc agaagtgcaa cagttgattt cggagagtta aagctcaaca
      601 tcggaattgc tcacggactc ggcaatgcca gaaaattgct cgatgatgta cgctccggaa
      661 aatcaaaatt ccatgcaata gagataatgg catgccccgg tggatgtata ggaggaggag
      721 gacaaccatt acaccatgga aactccgaaa tacttaaggc tagagctgca gctatctacc
      781 aggaagatcg caacaaaccg cttcgtaaat cacacgagaa tccggtcata ataaagctct
      841 atgaagaatt tctgggcaaa ccaaatagcg agaaagcaca taaactgctg catacacact
      901 attttgaaag aaagaagaaa ataattgtta aaccgtaaaa attcttcaat aaaaaataaa
      961 aagccatgtc aagcataaaa atagaattgc gtaaagaaga tgttgaaaag ataaaagaga
     1021 tctgtgcctc tttcaacaac gacccccagg aacttattaa cgttctacac aaatgccagg
     1081 aacatttcgg ctatcttcct gcagaagtac aggaagttgt atccgactgc cttaacgttc
     1141 ctgtggcaaa aatatatgga gtcgtaactt tctattcgtt ctttactatg actccaaagg
     1201 ggaaatatcc aatcagcata tgcatgggca cagcatgcta tgtgcgagga gctgaaaagg
     1261 ttctcgaaga attcaaaaaa gaactcggga tacaggtggg gcaaacaaca cccgacggta
     1321 aattctcact ttcgagtctg agatgtgttg gtgcatgcgg acttgcaccg gttgtgcttg
     1381 ttggggagaa gacatatggc agagtagcac ctgacgatgt aaaaaatatc ctgaaagagt
     1441 acgaataaca gatagtttct tctactgagt ttttccatgg agaatccggt cttaagccgg
     1501 attttctttt tattcactgc ttaaccccct gaaaatagaa tggattcatg tcatataata
     1561 aaattgacat caaggctttc attacttttt tttgaatttt ctgttcaatc catgcccttt
     1621 atattttgta aaaaacatat ttttctgcct ccttttacct tcaatatcgc gttcaacaaa
     1681 tattttcctg cctgtataag ggtctttacc aagataatac atcattgtgg aaagtgtcat
     1741 tggtgtagag gtaaagtcct gcacctgttc gggtctcaga ccaagcggtt ctattttgtt
     1801 tacaagcctt tgcatgtcgt caatggtaca gcccggatga gatgaaataa aatatggcac
     1861 aagctcataa ttgagtccat tttcttttac agtttcatca aaaatctttt tcagtttgat
     1921 gaacaaagag aaagggcttt tcctcatcag tccaacaata tgatcctcag tatgttctgg
     1981 cgccacttta agacgacctg aaacatggtt gattatcaat tctttaagat attctttctc
     2041 tgcttttccg gcctggggat tccattcttt gaaaagaagg tcgtatcgca ccccgctacc
     2101 gacaaatgct tttttgatat cttttctgct gttaaccatc ctgtaaagtt tcagcagtct
     2161 ctcgtgtgat gtattcagat tatcgcatac ggaaggccat atacatgaag ggcgcgaaca
     2221 cttcctgcat aattcctcat ttttaccttt catcccgtac atatttgccg atggtccgcc
     2281 aagatctgac agataacccc tgaatgaagg taatgcagat attctgtcaa tctcctgcat
     2341 tattgattct tctgtcctgt taataacaaa cttaccctga tgggcagata tagtacagaa
     2401 actacagcct ccgaaacatc cgcgatggat atttactgaa aactttatca tctcataagc
     2461 cggtatgggc tctttttttc tgtaccgggg atgtggctga taggtgaatg gcagagaata
     2521 tatcctgctg gcttcagttt cattgaatgg agggaaaggc ggattgacta tcacattata
     2581 ttttccgcaa ggctctataa gacgtgcggc gttaacctta ttcgattccc tttcgaaaac
     2641 tataaaatta tcagcaaatg catttttgtc ggcaacacac ttttcatatg aattcagaaa
     2701 tctgtctttc cagtcttttc ttgcaggcaa aggctcatcg gcaggaagtt taatgaccgt
     2761 ctgtggaacc gttttaagtg acgaaaacgg aacccctttt ttaagcaatc ttacaatttc
     2821 tataattggc tgttcgccca taccataaac aagcatgtcg gcgccgcttg aaatcagaat
     2881 tgaaggttct atgctgtctt tccagtaatc ataatgggtg agccgtctca ttgaggcttc
     2941 aattccccct ataacaacag gaacatctgg atagagactt ttaaggattg atgtataaac
     3001 tattgtaggg taatcggggc ggaatccggc tttgccacct ggtgtatagg catcgtcgga
     3061 gcgaagtctt ctgaaggccg tgtaatgatt caccatcgaa tccatgtttc cggctgttac
     3121 tccgaacaag taaactggtg ggccaagctt tttaaaatca cgcaggtcat ccttccagtt
     3181 gggttgcggt acaatagcca ctctcatacc ttctgcctca ataacccttg caataattgc
     3241 agcaccaaac gatggatggt caacataggc atcgccggtg aaaattatta catcggcctg
     3301 atcccaccca agagccttca tctcatcggc acccgtcggc aaccatttgt acttttcaga
     3361 aagcaaatcg acaaatttat ctttataaca actgcaaaga ttgtaaaaga aaatgaacta
     3421 ttgtcagttt tttggttccg gcaagattgt tgtaataatt tacaatgcac attattaata
     3481 aaatgtgtaa attgttataa aaacggaggg ggtgctatgg atatcagaag aattaccgac
     3541 cagcataaag cggtatgcag gcttgtggcc gacaagaagg tgaagaatag tctggaagta
     3601 ctaaacagca tgatctcagt ttccggaagg ggtcaattcc gggatgagta tgaaaacctc
     3661 gccatgacgt accggaatat tcttcaatac accatcgaag gagtaaaaga tcccgaaagg
     3721 gataaaatct acaatatact tctgaggtca attatcaacc tcgccgaccg tgtaaaacag
     3781 gacattctgt catattattc aggatggtac gcatact
//
LOCUS       DAOSNB010000259         3795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24217_length_3795_cov_4.342781, whole genome shotgun sequence.
ACCESSION   DAOSNB010000259 DAOSNB010000000
VERSION     DAOSNB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24217_length_3795_cov_4.342781"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1350
                     /locus_tag="PLG42_09515"
     CDS             148..1350
                     /locus_tag="PLG42_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOU31485.1"
                     /translation="MLLLLIISEFLTFAVIQIHLKGHSRTRYYLAMLFNTVLSIYLWA
                     LYIELNSFRGLYDDQFHIWLKMSFNGALCAIFIPRIILIGFHFAGMLIKIKEGGHIRK
                     LTDAGFVLWFIIFGVVVYGSAYGRFDVRTEEVTIHKESLHPDLEGFTIALISDLHLAG
                     FYNHQKFLKKQMEVINSYSPDIVVNSGDFVSYGWRESNGMDTILAVAYGRLGNYAVFG
                     NHDMGTYLPAADYTERESNILMIRDFIHKAGYQLLTDNNVIIRKGEASVSIAGITTRG
                     RHGHIKYGNLSKALEGTENADFRILISHDPNHWRKEVAGKTGVDLTLAGHTHGMQIGI
                     FTKKFRWSPSRYFYPEWGGLYHEGGQWLYVNRGLGVLGIPFRIWMPPEITIIRLTGNL
                     QQKAISSF"
     gene            1379..2047
                     /locus_tag="PLG42_09520"
     CDS             1379..2047
                     /locus_tag="PLG42_09520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOU31486.1"
                     /translation="MFEYRIIRSSRRTLGIVVSPEKGVVVRAPYRATVEEIRKFVEEK
                     SEWIERHLTIFGEMKSLSHTGKDGEHIMFRGREYRVSVMQSDIINVTLKDGILEVGSP
                     NPEKISATKAIIELWYRHNAEVILRNMFKDVLFRYSVQNFKPAQLTIRKMKGKWGSCT
                     SKGRITLNSELIKLNDSLVEYVIIHELCHLKHPNHGEGFYSLLGELVPQWQEMRKQLK
                     KYVF"
     gene            complement(2077..3477)
                     /locus_tag="PLG42_09525"
     CDS             complement(2077..3477)
                     /locus_tag="PLG42_09525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOU31487.1"
                     /translation="MLLKFLGAAREVTGSKILLTTDLGKKILIDCGMFQGKGLETDAM
                     NRNLGFEPSELDHVILTHAHIDHSGLIPYIHRLGFEGSVVCTSATRDLCSIMLADSGH
                     IQEQDTHTFNKKRLKKGLPAVTPIYTQEDAVECMKRFIGIPYDLKFKIDENIKVKFTN
                     TGHMLGSGVATLQIIENGQIKRIAFTGDIGRPVNRIIAPPKPFPQTDWLISESTYGNR
                     LHPAQEDAEKELLEIVIDTCVNKGGKLIIPAFSVGRTQEIVYSIHNFYKSGLLPKIEV
                     FVDSPLAVNATEIFRMHPECFNREVLESMEKDPDPFGFNALHYITKEEDSKRLNDYDK
                     PCIIISASGMMEAGRVKHHLANSIQDSKNTVLAVGYCAPQTLGARLLDGAKEVSIHGT
                     VYKVNADIRKIESFSGHADYQEMISFLQCQDPSALEKVFLVHGDYEAQKFFAEKLTEA
                     GFKNISIPDKFQEFEI"
BASE COUNT         1079 a          726 c          862 g         1128 t
ORIGIN      
        1 ggctcatgga agacaatcct gacccgaggt aaattcatag aatatagcgt tttacgtggt
       61 cataaaacca aaattcaggt agtaaggata agatattggt aatttttgcg gtaatttaag
      121 tatttttgcc gggaaatatt tttcctgatg ctccttcttc tgataatttc agaatttctg
      181 acctttgccg tcatacaaat ccatttaaag ggccactcaa gaacaaggta ctatctggca
      241 atgctcttta acacggttct gagcatctat ctatgggcac tttatattga acttaactca
      301 ttcaggggac tttatgacga ccagtttcat atatggctga aaatgagctt taacggagca
      361 ttatgtgcca tatttatacc gaggattatt ttgataggat ttcattttgc cgggatgctc
      421 ataaaaataa aagaaggagg ccatatacga aaactcacag atgcaggctt tgtattatgg
      481 tttataattt ttggagtggt cgtttacggt tcagcttacg gcaggttcga tgtaagaaca
      541 gaggaagtca ctatacataa ggaaagtctt catccagacc ttgaaggatt caccattgca
      601 ctgatatccg accttcatct tgccggattt tataatcatc agaaattcct gaaaaaacag
      661 atggaagtaa ttaactctta ttctcccgac attgtagtta actcgggaga tttcgtgagt
      721 tatggctgga gagaatcaaa tgggatggac accattctgg cagtagcgta tggtagactt
      781 ggaaattatg ctgtttttgg caatcatgac atgggaacct acctgcccgc tgcagactat
      841 accgaaaggg aatctaatat tctgatgatc agagatttta ttcataaagc tggttaccag
      901 ttactcactg acaacaatgt cattatcaga aaaggagagg cttcagtcag tattgccggc
      961 atcacaacca gaggaagaca tggtcatata aaatacggaa atctctcaaa agcacttgaa
     1021 ggaacagaaa atgccgattt cagaatatta atatcacacg atcccaacca ctggaggaaa
     1081 gaagtggcag gtaaaaccgg tgttgacctg acacttgccg gacatacaca cggcatgcag
     1141 attggtattt tcacaaaaaa attcagatgg agtccttcca gatattttta tccggagtgg
     1201 ggtggattat accacgaagg aggtcaatgg ctttatgtaa accggggact tggtgtgctg
     1261 ggcattccct tcaggatatg gatgccgcct gagataacaa taatcagact gaccgggaac
     1321 ctccagcaaa aggccatatc atcattttaa acttatctat taactcctat tttaattcat
     1381 gtttgaatac agaattatac gctcttcacg ccggaccctt ggaatagttg taagccctga
     1441 aaaaggtgtt gttgtaaggg caccttaccg tgcaactgtt gaagaaatca gaaagttcgt
     1501 tgaagaaaag tctgaatgga tagagagaca tcttaccata tttggcgaaa tgaaaagtct
     1561 gagccataca ggcaaagatg gagagcacat aatgttccgg ggcagggagt accgggtttc
     1621 cgttatgcag tcagatatta ttaatgtgac actgaaagat gggattctgg aagtcgggtc
     1681 acccaatcct gagaaaatat cagcaacgaa ggcaattatt gaattatggt acagacacaa
     1741 tgctgaagtc attttgagga atatgttcaa ggatgtactg ttcaggtact ccgtccagaa
     1801 ttttaaacct gctcaattga caatcaggaa gatgaaagga aaatggggaa gctgtaccag
     1861 caaaggcagg ataaccttaa actcagagct tataaaactc aatgattctc tggttgaata
     1921 tgttataata cacgaactat gtcatttaaa gcatccgaat catggagaag gtttctatag
     1981 ccttcttggt gagcttgtcc ctcaatggca ggaaatgaga aagcaattaa aaaaatacgt
     2041 attctaaaac attctaagtt aacagtaata attatctcag atttcgaatt cctggaattt
     2101 atcgggtatt gaaatatttt taaatccggc ttctgtaagt ttttctgcaa agaatttctg
     2161 ggcttcgtag tcgccgtgta caagaaaaac tttttcgagt gcagaaggat cctggcactg
     2221 aaggaaagat atcatttcct ggtaatcggc atggccgctg aatgattcta tcttacggat
     2281 atcggcattt accttgtaaa ctgttccgtg tattgaaacc tcctttgcac catcgaggag
     2341 tcttgcaccg agagtttgag gtgcgcagta acccactgca agaacagtat ttttactgtc
     2401 ctgaatgctg ttagccaggt ggtgttttac gcgtccagct tccatcattc ccgatgctga
     2461 gatgatgata catggtttat catagtcatt cagccttttg gagtcttctt cctttgttat
     2521 gtagtgaagg gcattaaagc cgaatgggtc ggggtccttt tccattgatt cgagtacttc
     2581 acggttgaag cattccgggt gcattctgaa tatttcggta gcgtttactg cgagggggct
     2641 gtcgacaaag acctctattt taggcagcag gccgcttttg taaaagttat gtattgaata
     2701 aacaatctcc tgtgtgcgac ccacgctgaa agctggtatt ataagttttc ctcctttgtt
     2761 gacgcatgta tcgataacaa tctcaagcag ttctttttct gcatcctctt gagccgggtg
     2821 caggcggttg ccgtatgttg attcagagat aagccagtcg gtctggggaa aaggtttggg
     2881 aggtgcgata attctgttta caggccggcc tatgtcgcca gtgaaggcta ttctttttat
     2941 ctgtccgttt tcaataatct ggagggttgc cacaccgctg ccgagcatat ggcctgtgtt
     3001 tgtgaactta accttaatgt tttcatcaat tttgaatttc aggtcgtatg gaatgccgat
     3061 aaatcttttc atacattcta cggcatcttc ctgggtatat ataggggtta cggccggaag
     3121 acccttttta agtctctttt tattaaatgt atgggtatcc tgttcctgta tgtgtcctga
     3181 atcggcaagc attattgagc agaggtcgcg tgtagcgctg gtgcatacca ccgagccttc
     3241 gaagcccagc cggtggatgt atgggatcag tccggagtga tcaatatgtg catgagtaag
     3301 tattacatgg tcgagttcgg aaggttcgaa gccaaggtta cggttcatgg cgtcggtctc
     3361 gaggcctttt ccctgaaaca ttccgcaatc gataaggatt tttttaccaa ggtcggtggt
     3421 taacagaatt ttgctgcctg ttacttcgcg tgcagcacca agaaatttta aaagcatgtc
     3481 gtttgatttt aaagaatcgt taaaggtaaa aattttaatc aattttttcc ctgatataac
     3541 aattctgtat atcctgctgg caataaaatt caatcttttg attttaccca tttttccctg
     3601 cccggcttat accaatcaga catgccccat gggaaaggac acacaggctg ctccttcgaa
     3661 gctaataatt taaaaatgaa taatattcaa gctccttcta agggggttgg gggtagcccc
     3721 ctgccagggg gttgggggta attcttttgc acagggctca gggcacaggg ctcaggtcgt
     3781 acgtattgaa gtaat
//
LOCUS       DAOSNB010000260         3767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24436_length_3767_cov_4.216595, whole genome shotgun sequence.
ACCESSION   DAOSNB010000260 DAOSNB010000000
VERSION     DAOSNB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24436_length_3767_cov_4.216595"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLG42_09530"
     CDS             <1..795
                     /locus_tag="PLG42_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOU31488.1"
                     /translation="NEISNLRYSGISVGWIWGYAYSPSKRNLIAYNHIHHLGWGELCD
                     MGGVYTLGASEGTVVSNNVIHHVYSFDYGGWGLYTDEGSYGIVMEKNLVYLCKNSGFH
                     QHYGKENIIRNNIFAFNIRAQLQATRVEEHRSLSFTNNIIYFDRGTLTSSNWHKFNLF
                     TDYNCYWDIRTKELRFGDRTFAEWQKEGKDLHSVIADPMFVDPVKFDFRFKNLSVARR
                     IKFVPFDYSLAGIYGDEEWKKKAVLDPELIKKFDERVTEMEKQVKK"
     gene            861..1739
                     /locus_tag="PLG42_09535"
     CDS             861..1739
                     /locus_tag="PLG42_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOU31489.1"
                     /translation="MKTLILTILVALFPALVFSQQERKINVIVFGAHPDDCDGKVGGT
                     AIEFAKRGHNVLFVSVTDGSAGHHEYGGAPLANTRKLEAQEAGRRFGVKYLVLDHPDG
                     KLMPTLDVREEIIRLIRQWNADVVIVHRPNDYHPDHRYTSTLVQDAAYMVIVPNIVPD
                     TPPLKKNPVFLYMEDNFKKPQPFTPDIVIDITDSWDQKMYALAAHTSQMFEWLPWTNG
                     TLSQVPAGEKERLEWLKKTRGPGQISPEKRAALEKWYGKERAQKAKIVESFEICEYGR
                     RPSEDEIRQLFPMMVK"
     gene            1841..3415
                     /locus_tag="PLG42_09540"
     CDS             1841..3415
                     /locus_tag="PLG42_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOU31490.1"
                     /translation="MTNLNKAGSYLLTIVRIAIGWQFLYEGIAKIIAGNWTSAPYLAG
                     SKWILAPLFTAMADNQAAVAFVDFINIWGMVLVGVGLMLGLLSRWASAGGALMLLLYF
                     MAYPPIPGYMTGVPTEGSYLWVNRNLIELFVLAIFVFISPASHYGLDRLIKRWKEERA
                     RQPVPEPSGGKEAASQRRELLRDLISVPAIGAFAYALYRKRKWDSFEERLLKVQGIDA
                     NTGATLLTFTYTSLKELKGQIPKGVIKYNGKDGSQSQFELSRIIMGGNLIGGWAHARD
                     LIYVSKLIKTYHTDEKVMQTLALAEKCGVNALLTNPQLGRIFQKYKHEFRSKMKFISD
                     CGVGLDFQKGIAMSLAVGADALYCQGEITDRWTDENWKDPQKRTLEQRVELISKGIEE
                     IRSHGKPAGIGAHKIEAIKKCVEYGIKPDFWVKTCHSHNYWSAKANPEWHDNIFDYDP
                     EETIRYMATLEEPWIAFKVLAAGAIKPEEGLKYAFSNGADFVCLGMYDFQIVEDINIA
                     LDTLSSITERQRPWRG"
BASE COUNT         1182 a          732 c          888 g          965 t
ORIGIN      
        1 aatgaaatat caaatctcag gtattcaggc atttccgtgg ggtggatctg gggctatgct
       61 tacagtccgt cgaagagaaa cctgattgca tataatcaca tacatcacct tggctgggga
      121 gaattatgtg acatgggagg agtatataca cttggtgctt cagaaggaac ggttgtttca
      181 aacaatgtga ttcaccatgt ctattctttc gattatggcg gctggggact ctataccgac
      241 gaaggctctt acggaatcgt tatggaaaag aatttggttt atctatgtaa aaactccggc
      301 ttccaccagc attacggaaa agaaaacata atccgcaaca acatatttgc atttaacata
      361 agagcacagc ttcaggcaac aagggttgaa gaacacaggt cgctgagttt taccaataat
      421 attatatatt tcgacagggg gacacttact tccagtaact ggcataaatt caaccttttt
      481 accgattata actgctactg ggatatacgc acaaaagaat taagatttgg tgacaggaca
      541 tttgcggaat ggcagaagga aggaaaggat ctgcattcag ttattgccga tcccatgttt
      601 gttgacccgg taaagttcga cttcagattt aagaatcttt cggttgcacg cagaataaaa
      661 ttcgttccgt tcgactattc acttgcaggc atttatggtg atgaggaatg gaaaaagaaa
      721 gctgtgcttg accctgaact gataaaaaaa tttgacgaaa gagtgacaga aatggaaaaa
      781 caggtaaaaa aataaatgta aattgtcaaa aaatgatttt taatggataa acctgaacta
      841 acctgaaaaa taggagatct atgaaaacac tcattttaac aattctggtg gcattatttc
      901 ctgcacttgt tttttcccag caggagagga agatcaatgt aattgtcttt ggggctcatc
      961 ccgatgattg tgatggcaag gtaggaggta cagcaattga gtttgcaaaa aggggacata
     1021 atgttctttt tgtatcggtg actgacggca gtgcgggtca ccatgaatat ggaggggcgc
     1081 ctcttgcaaa cacacgaaaa cttgaagcac aggaagccgg tagaaggttc ggagttaagt
     1141 atttggtgct cgaccatccc gatggtaagc tcatgccaac ccttgatgta agggaagaga
     1201 ttatcaggct gattcgccag tggaatgccg atgtagtaat cgttcacaga cccaatgatt
     1261 accaccccga ccaccgctat acttctacac tggtccagga tgcggcatac atggttattg
     1321 ttcctaatat tgttcccgat acaccacccc ttaaaaagaa cccggtcttc ctctatatgg
     1381 aggataattt caaaaagcct cagcccttta ctccggatat tgtgatagat ataaccgatt
     1441 cgtgggacca gaagatgtat gcacttgcag cccatacctc acaaatgttt gagtggcttc
     1501 cctggacaaa tggcacgctt agccaggttc cggcaggtga gaaagagagg cttgaatggc
     1561 tgaagaagac acggggccca gggcagattt cacctgagaa aagagcagca ctcgaaaaat
     1621 ggtacggaaa agaaagagcc cagaaggcga aaatagttga atcattcgag atatgcgaat
     1681 atgggcgccg tccttccgaa gacgaaataa ggcaactctt cccgatgatg gtgaaatagt
     1741 ttgctcataa cggaacagat gccaccattc aacctcccct ttgaggggag tgaaaagaat
     1801 taaattgaca aagtgatttt atgtaacaaa tactttttac atgacaaacc ttaacaaagc
     1861 tggaagttat cttttaacaa ttgtaagaat tgccattggc tggcaattcc tttatgaagg
     1921 tatcgccaaa attattgcag gaaactggac gtcagcacct tatcttgcag gctcaaaatg
     1981 gatacttgcg cctcttttta cagcaatggc tgacaatcag gctgctgttg cctttgttga
     2041 ttttataaac atatggggta tggtgcttgt aggagtcgga cttatgcttg gtcttctgtc
     2101 gagatgggca agtgcaggcg gagcattgat gcttctactc tattttatgg cgtaccctcc
     2161 tatacccgga tatatgaccg gagtacccac tgaaggcagt tatctgtggg taaacagaaa
     2221 ccttattgaa ctatttgtcc tggcaatttt tgttttcatc tctccagctt cacactacgg
     2281 actcgacaga cttataaaga gatggaaaga agagagggcg cgtcagcctg tgcctgaacc
     2341 ttcaggaggt aaagaagctg cctcacaacg tcgcgaactg ctgagggatc ttatatccgt
     2401 tcctgctata ggtgcttttg catatgcatt ataccgcaag aggaaatggg acagctttga
     2461 ggaaagactg cttaaggtac agggcattga tgcaaacacc ggtgcaacac ttcttacctt
     2521 cacttatacc tctttaaagg aacttaaagg tcaaattccg aaaggagtta ttaaatataa
     2581 cggtaaggat ggaagtcagt cgcagttcga actcagccgt atcataatgg gaggtaatct
     2641 tatcgggggc tgggctcatg cacgtgacct tatttatgtg tcgaagctga taaagacata
     2701 ccataccgac gaaaaggtaa tgcagactct tgcactggct gaaaaatgtg gtgtgaacgc
     2761 attgctgaca aacccgcagc tcgggagaat atttcagaaa tataagcatg aattcagaag
     2821 taaaatgaaa tttatctccg actgcggagt aggactcgat tttcagaagg gtattgcaat
     2881 gtcgctggcc gttggtgccg atgctcttta ttgccagggc gagattacag accgctggac
     2941 agatgaaaac tggaaagacc ctcagaagag aacacttgaa cagcgggtgg aactgataag
     3001 caaaggcatc gaagaaatca gaagccacgg caaacctgca ggaataggtg cccacaaaat
     3061 tgaggcaata aagaagtgtg tagagtacgg aattaaaccg gatttctggg taaaaacctg
     3121 ccatagccac aactactggt cggctaaagc aaaccccgaa tggcacgaca atatttttga
     3181 ttatgacccg gaggaaacaa tcaggtatat ggcaactttg gaagagccat ggattgcctt
     3241 caaagtgctg gcagccggag caataaaacc ggaagaaggc cttaagtatg ccttctcaaa
     3301 cggagccgat tttgtgtgcc tcggaatgta cgatttccag atagttgaag atatcaacat
     3361 tgctctcgac actttatcgt caataactga aaggcagaga ccctggaggg ggtaggttgt
     3421 taatctgaga ataataaact aaaagattta atataaaatt tcacgcaaag gccacgaagt
     3481 tcacaaagaa aaaaatcttt gtgttctttg tgctctttgt gtgagacata agaaattttt
     3541 gagataaaag aagaaagaaa aaagaataat tcacacagag gccacaaagg gcacaaagaa
     3601 tataccgaag aattgcagat ggagaaaaat tacattttat gagacgcttg cctttagtcc
     3661 atgttgcttt tttcacctcc ggactattgt cgtgtgccgt gaaaagggac ctgatccgga
     3721 aatatgggaa acatatccgc gtctgcaggg agaaatacgt aaatttc
//
LOCUS       DAOSNB010000261         3762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24477_length_3762_cov_3.266253, whole genome shotgun sequence.
ACCESSION   DAOSNB010000261 DAOSNB010000000
VERSION     DAOSNB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24477_length_3762_cov_3.266253"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2562)
                     /locus_tag="PLG42_09545"
     CDS             complement(<1..2562)
                     /locus_tag="PLG42_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU31491.1"
                     /translation="MKLVVLFLAVGLVIQPAYSAGGIDDQQQIRITGTVTDTETGNPM
                     PGVNIQVKGTTIGAITDADGKYVINVTDRNAILVFSFIGYDTKEVPLAGKVVLDVAMS
                     PTVTDLDEVVVVGYSTQKKANVIGSVTSLPGAQIQSLPTPTVAQALSGRLPGSIIIQM
                     NDEPGQNTTRILVRGRSTLGGSRASLSSATAPLVIIDGVPGRSMNEIDPNDIETLSVL
                     KDASAAIYGAQAANGVILITTKKGSEGAPKLNYQFYQGYMTPTIIPEVCDAAEYATML
                     SEYQVATGKPRTYTDQDIELFRSGADPWQHPNTNWYKELIKNWTGTTRHNLSIDGGFR
                     GMTYYFSAGYKLDDAFYKASSTKYKQFNVRTKLDLPITSWLKVGTELAGFQINRLYPY
                     KSADAIVGQSTRLVPTTWAYWPNGLPGPDIEYGDNPVVTSTFEGGKDDQKTYRVLTTF
                     NGTITVPFVKGLTLNGSYSYDLTNFYRKRFFKPWILYYPDWASAKRDPNTGFITDMNL
                     IPTPRGLSYPQNHEDYSRTITNTILLSVNYTRKFGDHSLTLYGGFEQYTYDYNNLYGY
                     RTYYISDLIQTMDAGADQDKNTTGGRSIYARKSYIGRASYDFRGKYLAEIVFRADGSL
                     KYPPSSRWGYFPGFLLGWRASEEGFWKDNISFINYFKLKASYGSMGMDPGSAFQYMNS
                     FGLSSGMSFGTSATIETAVGPPTVANPNITWEKQTTKNIGFESKWFRDLIHLNFEYFY
                     NIRKDILATRDASVPAFTGLSLPQENIARVDNRGFEVEAGVHKNITPDLRIDLGGNFS
                     YNHNEVVFMDEPAKAVPWQQLTGHPYNAWLMYKAIGIFEDDTYTVNGVANYPRWSG"
     assembly_gap    2727..2736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2883..>3762)
                     /locus_tag="PLG42_09550"
     CDS             complement(2883..>3762)
                     /locus_tag="PLG42_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOU31492.1"
                     /translation="MGSPPDEPFRRSDEGPVREVQVSSFFMGEIEVTWDEYLAFYAQT
                     AAEGRSTDTEGIRYKGASADVDAITGPTPPYGQPDQGWGMGQRPAISMTYHAAETYCK
                     WLSKVTGKTYRLPTEAEWEYACRGGTSTPYFFPGNPEKFQRTGFKAKISKNDTTVINS
                     YVIYRENSGLRTHLPERVKPNPFGLKNMLGNVAEFCSDWYQPDVLAQYPEGLIIDPTG
                     PESGKEHVVRGGSYLDMAGRTRCAARDYTRTEEWLKTDPQMPKSIWWYSDCFYVGFRV
                     VCEFDEKTGNTESGKN"
BASE COUNT          980 a          892 c          771 g         1109 t
ORIGIN      
        1 tcctgaccag cgaggatagt tggcaacacc atttacagta taggtatcat cctcgaagat
       61 tccgatagcc ttatacatta accatgcatt atacgggtgc cctgtgagct gttgccacgg
      121 aactgccttt gcaggctcgt ccataaatac cacttcgtta tggttgtagc tgaagttgcc
      181 tccgagatca attctcaggt cgggtgtgat gtttttgtga acacctgctt caacttcaaa
      241 tcccctgttg tccactcttg caatgttttc ctgaggaagt gacaatccgg tgaatgcagg
      301 cactgaagca tcccttgttg caagaatatc ctttcttatg ttgtagaagt attcaaagtt
      361 caggtgtata aggtctctga accactttga ttcaaacccg atattctttg ttgtctgctt
      421 ctcccatgta atattcggat ttgcaactgt cggaggtcct accgcagttt caattgtagc
      481 acttgtaccg aatgacatac cggaagaaag accgaatgaa ttcatatact ggaatgcact
      541 accaggatcc atacccatac ttccatagga tgctttcaac ttgaaataat tgataaatga
      601 aatattgtct ttccagaacc cttcttcaga tgctctccat ccaagcagaa atcccgggaa
      661 gtaaccccaa cggctcgatg gcggatactt aagtgaacca tctgccctga acacaatctc
      721 agcaagatat tttccgcgga aatcgtaact ggcacggcct atatacgatt tacgtgcata
      781 tatcgatctg ccgcccgttg tattcttgtc ctggtcagca cctgcatcca tagtctgaat
      841 caggtctgaa atatagtatg tccggtaccc gtaaaggttg ttgtaatcgt acgtatactg
      901 ctcaaagcct ccatacagag ttaatgaatg atctccgaat ttcctggtgt agtttacact
      961 tagcagtatt gtgttggtaa ttgtcctcga atagtcttca tggttctgcg ggtacgaaag
     1021 acctctcggc gtcgggataa ggttcatgtc ggtaataaag ccagtgttcg gatcacgttt
     1081 ggctgaagcc cagtcgggat agtaaagtat ccacggtttg aagaacctct tgcggtagaa
     1141 gttggtcagg tcataactgt aactcccgtt aagtgtcaag ccttttacaa aaggtaccgt
     1201 aattgtaccg ttgaatgttg taagcactct gtatgttttc tggtcatcct taccaccttc
     1261 gaatgttgaa gtaaccaccg ggttgtcgcc gtattcgata tcaggtccgg gcagtccgtt
     1321 aggccagtat gcccatgttg taggtacaag acgtgtagat tgtcctacaa tagcatcggc
     1381 actcttgtac ggatagagtc tgttaatctg gaaccctgcc agctctgttc caacctttag
     1441 ccagcttgtt ataggcagat caagttttgt acgcacatta aactgtttgt actttgtcga
     1501 tgatgcttta tagaatgcat catccagttt ataacctgct gagaaatagt aggtcattcc
     1561 cctgaaacca ccgtcaattg aaaggttgtg gcgtgttgtt cccgtccagt tcttaatcag
     1621 ttccttatac cagttggtat tcgggtgctg ccacggatct gcaccagagc ggaagagttc
     1681 tatatcttgg tcggtatagg ttctcggttt tcctgttgct acctgatatt ccgaaagcat
     1741 tgttgcatat tcggctgcat cgcaaacttc aggaataatt gttggtgtca tatacccctg
     1801 atagaactgg tagttaagtt ttggggctcc ttcgcttcct ttctttgtgg taataagaat
     1861 cacaccgtta gcagcctgag caccgtaaat tgctgcagat gcatctttca gaaccgaaag
     1921 tgtctctatg tcgttagggt caatttcatt cattgaacga cccggaacac catcaataat
     1981 tacaagtggt gctgttgcag atgagagtga tgctcttgaa ccacccagag tactacgtcc
     2041 tctgacaaga atacgtgtgg tgttctgacc aggttcatca ttcatctgaa taatgattga
     2101 acccggcaga cgtccggaaa gagcctgtgc aacggtaggc gttggcaatg actgaatctg
     2161 agcaccgggc agcgacgtta ccgacccgat aacgtttgcc tttttctggg tagaataccc
     2221 tacaacaacc acctcatcaa ggtctgttac agttggtgac atggctacat caagtaccac
     2281 tttaccggca agcggtactt ccttggtgtc atatccgatg aaggaaaata ccaggattgc
     2341 attcctgtct gttacattga taacatattt cccatctgca tcggtaatgg caccaattgt
     2401 ggtcccctta acctgaatgt tcactccagg catcgggttg ccggtttcag tgtcagtaac
     2461 cgttccggtt attcttatct gctgctggtc gtcaattcca ccagctgaat aagctggctg
     2521 aattaccaga ccaactgcca gaaaaagaac aaccaacttc ataagcctga agagcttacg
     2581 aaagctattt cctggccctg agggccaaaa aaaacagatt gttttcttca taacgttaca
     2641 tcgttaattg gttaggaaaa taataaagta ttgatattat atgtaatcat aatatcctca
     2701 ttttataaac cccataccca acttttnnnn nnnnnncctc tgaatgcttt cccccgaatt
     2761 aatattgtaa aattaacact tattcaattc ctactttact actaaaccgt ttaaattaat
     2821 tccgaattta atatggataa attaaaaaaa caaatctaat ttaaaattta acatttcatt
     2881 cgctaatttt ttccagattc tgtattgcca gttttttcat cgaattcgca tacaactctg
     2941 aagcccacat agaaacaatc ggaataccac cagatgcttt tgggcatctg cggatcggtc
     3001 ttaagccact cttctgttct tgtataatca cgggctgcac atcttgtcct gcctgccatg
     3061 tcgagataag agccacccct tacaacatgt tccttgcccg attcaggacc agtggggtca
     3121 ataatcaaac cctccggata ctgagcaagt acatcgggct gataccagtc ggaacagaac
     3181 tccgctacat taccaagcat gtttttaagt ccaaaaggat taggctttac cctttcaggc
     3241 agatgggtcc ttaatccact gttctctctg tagatcacat atgaattaat tacagtggta
     3301 tcgtttttgg atatctttgc tttaaatcca gttctctgga atttctcagg atttccgggg
     3361 aagaaatatg gagttgatgt gcctcccctg catgcatatt cccactcagc ctcagtgggc
     3421 aaacgatatg tctttcctgt aacctttgaa agccatttgc agtaagtctc ggctgcatga
     3481 tatgtcattg aaattgcggg ccgctgaccc ataccccacc cctgatcggg ctgaccatac
     3541 ggtggagtgg gacccgtaat agcatcaaca tccgctgaag cccctttata gcggatacct
     3601 tctgtatcgg tactcctgcc ctcagctgcc gtctgcgcat aaaaagcaag gtattcatcc
     3661 catgtaactt caatctcacc cataaaaaac gaactaacct gcacctcccg taccggtcct
     3721 tcatcactgc ggcggaatgg ctcatcaggc ggacttccca tc
//
LOCUS       DAOSNB010000262         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24497_length_3759_cov_4.697354, whole genome shotgun sequence.
ACCESSION   DAOSNB010000262 DAOSNB010000000
VERSION     DAOSNB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24497_length_3759_cov_4.697354"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PLG42_09555"
     CDS             <1..200
                     /locus_tag="PLG42_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOU31493.1"
                     /translation="VSYLLVITDTKNTIVFKTSSWQDSWDGTKDGRPVPSGVYLWYLK
                     ITTPAGKKIQKSGTVTVIRNR"
     gene            278..2551
                     /locus_tag="PLG42_09560"
     CDS             278..2551
                     /locus_tag="PLG42_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOU31494.1"
                     /translation="MSKKDIFDEEDRKLIREEYESLMNNLYRCNKPGDRELIEKAFNV
                     ANEAHWNMRRRSGEPYIIHPLKVAKIVNQEIGLGAKSIATALLHDVVEDTDYTLEDVR
                     REFGDKIASLIDGLTKISGTYNTDISPSLQAENFRKMLLTISDDLRVLLIKIADRLHN
                     MRTLDSLPENKKIKVAGETIYLYAPLAARLGLYAIKTELEDLSFKFRHPKIYEEIQTK
                     LKHNEKKYHALINKFSLPIIEKLNDAGYKYEITGRPKSIYSIWKKMQTKNVPFEEIYD
                     ILAVRIVFEPFPGISEKTQCWNIYSMITDIYLPKPDRLRDWLSRPKPNGYEALHVTVM
                     GPEGKWVEVQIRSTRMDEIAERGYAAHWKYKGDDSQESEFDKWIKRIRVLLENPLEDP
                     AEFLDDFKMNLFSSEIMVFTPKGYLISLPKGATALDFAYEIHTEVGNKAIGAKINYKL
                     MPLNTVLNSGDQVEIITSDKASPEKEWLNFVHTPRAKAAIKNALKAESRDRIQKGMTI
                     LEEKLNEIGLAPTSETLKKIMIHYDVTNKEELYSKIGLGIITLDNLKKIVRKNPSKSI
                     IKYWDLKLIGPKKDKELSGTEAEATTQQVKFDTKNPFLLRENIEGDEQSYEIAKCCNP
                     IPGDEVTGYLSHEGTIIVHKRNCPVAVRLMSSEGNRIIAVKWTMHKLASFLARISISG
                     IDRIGLLKDITNIISDELKANIRNINVSVHDGIFEGSLDLYVHHTRDLNNLIMKISNV
                     KGVEKVRRVENFGEETL"
     gene            2622..3077
                     /locus_tag="PLG42_09565"
     CDS             2622..3077
                     /locus_tag="PLG42_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOU31495.1"
                     /translation="MTIEDTKKVVKQIFTEYLERKRCRKTPERYAILEEIYSRDGHFD
                     IESLYISMKNKKYRVSRATLYNTINLLLDANLVIRHQFGKNISYYEKAFQCSQHDHLI
                     DINSGKVIEFCDPRINEIIRTACEMNNFRPLFHSLYIYGVSESKKQESL"
     gene            complement(3099..>3759)
                     /locus_tag="PLG42_09570"
     CDS             complement(3099..>3759)
                     /locus_tag="PLG42_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31496.1"
                     /translation="RKMKRTIMIFLVVMFLVAGNGYSYSQETSSPSKEYKFTIKTNPL
                     AALGGPFWVVVIPVTGEYKAYFETKVTNKTSFQIGASYIGPSVLLNLDEITKSEDGEE
                     IKGVKTSGYRIQGTYKFFVSRDLNAPEGFYLAPNISYATASVKNKANPDDKINGTKLN
                     INGLIGYQLITSGGFTLDVFTGLGYVSRKYTVSGDFDSEEFKSKSSISVPFGLAFGYA
                     F"
BASE COUNT         1294 a          696 c          753 g         1016 t
ORIGIN      
        1 cggtttcata tctcttagtt atcactgata cgaaaaacac gattgttttt aaaacatcca
       61 gctggcagga ttcatgggac ggaacgaaag acggtaggcc tgttccgtcg ggtgtatatc
      121 tctggtacct gaaaattacg actccagcag gtaaaaagat tcaaaaaagt ggcactgtta
      181 cggtaatcag gaacagataa aggaatgcaa aagccccctc actccttcag taacaacaaa
      241 acgttaattg cactatattt gcatttgatg tttgaatatg agcaagaagg atatttttga
      301 tgaagaggac aggaagctga tcagggagga atatgaatct ctgatgaata atctttacag
      361 gtgcaacaaa ccgggtgaca gggagcttat tgaaaaagcc ttcaacgttg ccaatgaagc
      421 acactggaac atgcgtcgca ggtcgggcga accatatatc attcatcctc ttaaagtagc
      481 aaaaattgtc aatcaagaaa ttggtctggg tgcaaaatcg attgcaaccg ctcttctgca
      541 cgatgtagtt gaagatactg attatactct cgaagatgta agaagagaat ttggcgacaa
      601 gattgcttct ttgattgacg ggttgacaaa aatttcagga acctacaata cagatatttc
      661 tccttcactt caggcagaaa atttcagaaa aatgcttcta accatttctg acgacctgag
      721 ggtgctgctc attaagatag ccgaccggct gcataatatg aggacgcttg attcgctgcc
      781 cgagaacaag aagattaaag tagctggtga gaccatatac ctgtatgcgc cgctggcagc
      841 caggcttgga ttgtatgcaa taaaaacaga gcttgaagac ctgagcttta aattcagaca
      901 tccgaaaata tacgaggaga tacagactaa actaaaacat aatgaaaaaa aatatcacgc
      961 gcttataaac aagttttcat tgcccattat tgaaaaactg aacgacgcag gttataaata
     1021 tgaaattaca ggaaggccaa aatcaattta ctcgatatgg aaaaaaatgc agaccaagaa
     1081 tgttcctttt gaggaaatct atgatatact ggctgtacgg attgtttttg agcctttccc
     1141 gggcatatct gaaaaaactc agtgctggaa tatctactca atgataacag acatttacct
     1201 tcccaaaccc gacaggcttc gcgactggtt aagtcgtcca aaaccaaacg gatatgaagc
     1261 tcttcatgtg acagttatgg ggcctgaagg taaatgggtg gaagtgcaga tacgtagtac
     1321 cagaatggat gaaatagccg aaagaggata tgcagctcac tggaaatata aaggtgatga
     1381 ttcacaggaa agtgaattcg acaaatggat aaagagaata agggtactgc ttgagaatcc
     1441 acttgaagat cctgcagaat ttcttgacga tttcaagatg aatctcttct cttccgagat
     1501 aatggtcttc actccaaaag gttatctcat ttcgcttcct aaaggagcca ccgcgcttga
     1561 tttcgcatat gaaatccata cggaagtcgg taataaggct ataggagcaa aaataaacta
     1621 taagctgatg ccactcaata cagtccttaa cagtggcgac caggttgaaa taattacatc
     1681 tgacaaagca tcccctgaaa aggaatggtt aaattttgtt catacaccaa gggcaaaggc
     1741 agcaattaaa aatgccctga aagctgaatc gagagaccgg atacaaaaag gcatgacaat
     1801 tcttgaggaa aaactaaatg aaataggttt ggctccaact tccgagacac ttaaaaaaat
     1861 catgattcat tatgatgtta caaataaaga agaactatat tcaaaaattg gacttggcat
     1921 aattactctc gacaatctga agaaaattgt gcgtaagaat ccgtcaaaaa gtatcattaa
     1981 atactgggat cttaagctga taggcccaaa aaaagataaa gaattatccg gcacagaagc
     2041 tgaagcaaca actcaacagg ttaaattcga tacaaaaaat cctttcctgc tacgcgagaa
     2101 tattgaaggt gatgagcaat catacgagat agcaaaatgc tgcaatccta ttccgggaga
     2161 tgaagttact ggatacctgt cgcatgaagg aacaataatt gttcacaagc gtaattgccc
     2221 tgtggcggtc agattgatgt cgagtgaggg caacagaatt attgcagtaa aatggacaat
     2281 gcataaactt gcatcatttc ttgcaagaat cagtatttca gggattgaca gaatcgggct
     2341 tctaaaagat ataacaaaca tcatttcaga cgaactcaaa gcaaacatac gcaacataaa
     2401 tgtttcggtt catgatggca tttttgaagg atcgctggat ctttatgtgc atcacaccag
     2461 agatcttaat aatctgataa tgaaaatatc aaacgttaaa ggagttgaaa aggtgaggag
     2521 ggtggaaaat tttggcgaag agacattata gtgaatttca tggttttatg aggaagttct
     2581 taaataagca catggatttt aattatcaat tttggctata aatgaccatt gaagatacaa
     2641 aaaaagttgt aaaacagata ttcactgaat atcttgaaag gaagagatgc agaaaaacgc
     2701 cggaacggta tgccatactg gaagagatat acagtcgtga tggtcatttt gatattgaat
     2761 cgctgtatat ctcaatgaaa aacaagaagt acagggttag cagggcaaca ctttacaata
     2821 caataaatct tctgctcgat gccaaccttg tgataagaca ccagtttggt aaaaacattt
     2881 cctattacga gaaggctttc cagtgcagcc agcacgatca tcttatcgac atcaactcgg
     2941 ggaaggtaat agagttctgt gatcctcgca taaatgaaat aatcagaacg gcctgcgaaa
     3001 tgaacaattt caggccactg tttcactccc tgtacatata cggggtttct gagagtaaaa
     3061 agcaagaatc cctttaaaaa atttcttaaa tactcccgtc agaaagcata accgaaagct
     3121 aaaccaaaag gtaccgaaat actgcttttg cttttaaatt cttcactatc aaagtctccc
     3181 gatactgtgt attttctgga aacataacca agtccggtga aaacatcgag tgtgaaacca
     3241 cctgaagtaa ttaactgata accaattaat ccgttaatat ttaattttgt tccatttatt
     3301 ttatcatctg gatttgcctt atttttaact gatgcggtag cataagagat gttaggtgcc
     3361 aggtaaaatc cttcaggggc attaaggtct ctacttacga aaaacttata ggtgccctga
     3421 atacggtatc cgctagtttt aacaccttta atttcctctc catcttcact ttttgttatt
     3481 tcgtcaagat tgagcaacac acttggtccg atataactgg caccaatctg aaatgacgtt
     3541 ttatttgtga cttttgtttc aaaatatgcc ttatactctc ctgttaccgg gataactacc
     3601 acccaaaaag gtccgccaag cgctgcgagt ggatttgttt taatggtaaa tttatattcc
     3661 tttgaaggag aagatgtttc ctgagaataa ctatatccat ttcctgctac cagaaacatc
     3721 acaactaaaa aaatcattat tgttcttttc atttttcta
//
LOCUS       DAOSNB010000263         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24573_length_3751_cov_3.829004, whole genome shotgun sequence.
ACCESSION   DAOSNB010000263 DAOSNB010000000
VERSION     DAOSNB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24573_length_3751_cov_3.829004"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="rfbC"
                     /locus_tag="PLG42_09575"
     CDS             complement(<1..527)
                     /gene="rfbC"
                     /locus_tag="PLG42_09575"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOU31497.1"
                     /translation="MKITETKFKGLLIIEPQVFTDQRGYFMETFNMEVFRKAGIVFNP
                     VQDNESKSVRGVIRGLHYQLRPFEQAKLIRVVEGKILDVALDLRRESKTFGKWYSIEL
                     DAESKKQLFIPRGFAHGFSVLSETAIVQYKCDNYYNPSYERGILPIDPALGIDWNIEI
                     KEAIISEKDKNLPYL"
     gene            complement(545..1420)
                     /gene="rfbA"
                     /locus_tag="PLG42_09580"
     CDS             complement(545..1420)
                     /gene="rfbA"
                     /locus_tag="PLG42_09580"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOU31498.1"
                     /translation="MKGIILAGGSGTRLYPITNAISKQILPVYDKPMIYYPLSVLMLA
                     GIREILIISTPRDLPVFMNLLGDGSSLGISFSYAEQPHPGGLAQAFIIGEKFIGTDTV
                     CMILGDNIFYGHGFGDILLETAKLQEGACVFGYYVNDPQRYGVIEFNSLLEPLSIEEK
                     PARPKSNYAVTGLYFYDNSVISKAKSLKPSARGELEITDINLMYLQEGKLKLRLLGRG
                     MAWLDTGTFDSLLQASNFIATLEQRQGLKASCIEEIAYKRGFITKEQLIRLAEPYRNS
                     QYGQYLLRIANDDII"
     gene            complement(1465..2781)
                     /locus_tag="PLG42_09585"
     CDS             complement(1465..2781)
                     /locus_tag="PLG42_09585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOU31499.1"
                     /translation="MKIVVAGTGYVGLVTGTCFAETGVTVTCVDIDKEKIKLLQEGKT
                     PLYEPGLEPMMKRNIEKGRLSFTTDLEKSLEGSEAIFIAVGTPPGEDGSADLQHVLKV
                     ASEIGKNIKSYVVVVIKSTVPVGTSEKVRKIIREELEKRGVDIPFDMASNPEFLKEGS
                     AVEDFLRPERIVIGTENERAAEIMSRLYKPFVLNNHPIYYMDIPSAELTKYAANAMLA
                     TRISFINEIANLCELLGADINQVRKGIGSDSRIGNKFLYPGPGYGGSCFPKDVKALIR
                     TAAEKGYEMKVIAAVEKANEYQKNVLFFKIRNHFNNNLKGRTIGIWGLSFKPHTDDIR
                     ESPSIYLIEKLLQEGAIVKAYDPAAMEETKKVLGNRVKYASDQYEAVIKADALVLVTE
                     WPEFRLPDLKKMAETMRGKVIFDGRNIYDPLEVRKAGFTYYSIGRK"
     gene            3079..>3751
                     /locus_tag="PLG42_09590"
     CDS             3079..>3751
                     /locus_tag="PLG42_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOU31500.1"
                     /translation="MTKGKEKTIDLEGIEKNDSIENQEAEPSGKELNEELKEGVETLS
                     AAEPESEKVQLFEKSPVVSEKKEKPERKKAASEAGPKKSTSKERVASGKTRAKAEPAA
                     EAKEKVIEEKVMPGDEFSWEEFESKENRISPKYAEYEAVYDKTLSTIAEDQVVKGTVI
                     QMTNREVVINIGYKSEGVVSLNEFRYNPNLKVGDVVDVYVESTEDKKGQLVLSHKKAR
                     ALNSWE"
BASE COUNT         1100 a          777 c          701 g         1173 t
ORIGIN      
        1 cttaaataag gaagattttt atctttttca cttataatag cctctttaat ttctatattc
       61 cagtcaattc caagagcagg gtcgatgggc aggatgcctc tttcatatga aggattatag
      121 taattgtcac atttatactg aacaatagct gtttcgctga gcactgagaa accatgtgca
      181 aatcctcttg gtatgaaaag ctgttttttg gattctgcgt cgagttctat actgtaccat
      241 tttccaaaag tcttagattc tcttctcaga tccaatgcca catccaggat tttgccctct
      301 accactctta tgagttttgc ttgttcaaat ggccggagct gataatgtaa tccccttatt
      361 actcctctta cagattttga ttcgttatcc tgaactggat taaaaacaat tcctgctttt
      421 ctgaaaacct ccatattgaa agtttccatg aaatatccac gttgatctgt aaagacctga
      481 ggttcaataa ttagtagccc tttaaatttt gtttctgtta ttttcatgga ttctttgtaa
      541 catgttatat tatatcatca tttgctattc gaagcagata ttgtccgtac tgactgttcc
      601 ggtaaggttc ggcgagtctg ataagctgct ctttggtgat aaaacctctt ttataggcta
      661 tctcttcaat gcacgaagct tttagtccct gtctctgttc aagggttgca ataaagttgg
      721 aagcctgaag aaggctgtca aaagttccgg tgtcgagcca tgccatacct ctgccaagaa
      781 gccttagctt taattttcct tcctgaagat acatgaggtt gatatcggtt atttcaagct
      841 cgcctcttgc agaaggcttc agggattttg cttttgaaat aacactattg tcataaaaat
      901 agagacctgt tacagcataa tttgattttg gtctggcagg cttctcctct atacttaagg
      961 gttccagcag actgttaaat tctatcaccc catagcgctg tggatcgttt acataatatc
     1021 cgaaaacaca ggctccttct tgaagttttg cagtttcgag aagaatatca ccgaacccat
     1081 gcccatagaa aatgttatcc cccagaatca tacataccgt atcagttcca ataaattttt
     1141 ctccgataat gaatgcttgc gccagtccgc ctggatgagg ctgttcggca tatgaaaaac
     1201 ttattcccag agaagaaccg tctccaagaa gattcatgaa taccggaaga tccctcgggg
     1261 tggagataat taagatttcc cttattccgg caagcatcag caccgacaga gggtaataga
     1321 tcataggttt atcataaaca ggtaagatct gtttgcttat tgcattggtg atgggataaa
     1381 gcctcgtacc cgacccccct gcaagtataa tacctttcat gattggatga ttttataata
     1441 aatgttcaat gtcaatataa tatttcattt tctccctata ctataatagg taaatccagc
     1501 ttttctgact tctaacgggt cgtagatatt tcttccatcg aatattactt tccctctcat
     1561 cgtttcagcc atttttttta agtcaggaag tctgaattcc ggccattctg taaccagtac
     1621 cagtgcatcg gccttaataa cagcctcgta ctgatcagat gcatatttaa ccctgttgcc
     1681 aagaactttt ttggtttctt ccatggcagc aggatcataa gctttcacaa ttgcaccttc
     1741 ctgaagcagt ttctcaatca ggtaaattga aggagattcc cttatgtcat cggtgtgggg
     1801 cttgaatgat agcccccaga ttcctatcgt tcttccctta agattattgt tgaaatggtt
     1861 tctgatttta aagaaaagaa cattcttctg gtattcattt gccttttcca ccgctgcaat
     1921 gaccttcatc tcgtagccct tttcagcagc agttctgata agtgctttta catctttcgg
     1981 gaagcatgaa cctccataac ctggacccgg gtatagaaac ttattcccaa tccttgagtc
     2041 actgccaata ccttttctca cctgattaat atcggctcca agcagttcac acaagttggc
     2101 tatttcattt ataaaactga ttctggttgc cagcatggca tttgcagcat attttgtgag
     2161 ttcggcagag gggatgtcca tataataaat aggatggtta ttgagaacga aaggtttata
     2221 caacctgctc attatctcgg cagctctttc attttcggtt cctattacaa tcctttcggg
     2281 acgcagaaaa tcctctacgg ctgaaccttc cttcagaaat tcaggattgg atgccatgtc
     2341 aaacggaata tcaactccgc gtttctccag ctcctccctg ataatttttc ttactttctc
     2401 agatgtaccc actggcactg tgctctttat cacaaccaca acatatgatt taatgttttt
     2461 ccctatctct gatgctacct taagtacgtg ttgaaggtcg gcacttccat cttcgccagg
     2521 gggtgtgcct accgcaatga aaatggcctc tgagccttcc agactttttt ccaaatcggt
     2581 ggtgaatgat aaacgtccct tttcaatatt tctcttcatc attggttcca ggcccggctc
     2641 atatagcggt gttttgcctt cctgcaaaag cttaattttt tctttatcaa tatctacaca
     2701 ggttaccgta acacctgttt cagcaaaaca ggttcctgtt accagcccta cataaccggt
     2761 tccggctaca acaattttca taaagtttat tttaaatttt taatcagatt atccgttcct
     2821 gcaaagataa cacatcagat gaattaagaa aatccgccat taacatacaa tcgctttact
     2881 ctttttttca acagtggact tgaaaactaa agagatgttg tgttcaagct ggaaacagcc
     2941 cacagtcata aggtactatt tttttattaa aaaccgtctt gaatttatga tgaatcgaaa
     3001 aaataattta tctttgcaaa cctgtttaaa tcaatataat gtctaactta ttaattatta
     3061 gtttattaaa ttaaatttat gacaaaaggc aaagaaaaaa caatcgatct tgaaggaatt
     3121 gaaaaaaacg actccattga gaatcaggag gcggaaccat caggaaaaga attaaatgaa
     3181 gaattgaaag agggcgttga aactctttct gcagcagaac cggaatctga gaaggtgcaa
     3241 ttatttgaaa agtcacctgt agtttctgaa aaaaaggaaa agcctgaacg taaaaaggca
     3301 gcttctgaag ctggaccaaa aaaaagcact tcaaaagaga gagtggcatc cgggaaaacc
     3361 agagcgaaag ctgagccagc agcggaagcg aaagaaaaag ttatcgaaga aaaagtaatg
     3421 ccgggagatg aattcagctg ggaggaattc gaaagtaaag aaaatcgcat ttcaccgaaa
     3481 tatgctgaat atgaggctgt ttacgacaaa accctttcaa ctattgcaga agaccaggtt
     3541 gttaagggta cggttattca gatgaccaac cgtgaagtcg ttatcaacat aggctacaaa
     3601 tctgaaggtg tggtgagtct gaatgaattc agatacaacc cgaatctaaa ggttggtgat
     3661 gttgttgatg tttatgttga aagtacagaa gacaaaaaag gtcaacttgt tctttcccac
     3721 aagaaggcac gggctttaaa ctcatgggaa a
//
LOCUS       DAOSNB010000264         3751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24577_length_3751_cov_3.612013, whole genome shotgun sequence.
ACCESSION   DAOSNB010000264 DAOSNB010000000
VERSION     DAOSNB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24577_length_3751_cov_3.612013"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLG42_09595"
     CDS             complement(<1..263)
                     /locus_tag="PLG42_09595"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539140.1"
                     /note="catalyzes the formation of
                     3-(imidazol-4-yl)-2-oxopropyl phosphate from
                     D-ethythro-1-(imidazol-4-yl)glycerol 3-phosphate and
                     histidinol from histidinol phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase"
                     /protein_id="HOU31501.1"
                     /translation="MKRTLFIDRDGTIIKEPPDEQVDSLEKLEFVPGVITALAKIATQ
                     TDYELVMVTNQDGLGTELFPEETFWPAHNKMLKILEGEGIRFS"
     gene            complement(260..1291)
                     /gene="hisC"
                     /locus_tag="PLG42_09600"
     CDS             complement(260..1291)
                     /gene="hisC"
                     /locus_tag="PLG42_09600"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOU31502.1"
                     /translation="MPDINKLVRENIKRLVPYSSARDEYNGKPGVFLDANENPYGTLN
                     RYPDPYQQNLKSAVSRLKGINKEYIFIGNGSDEIIDLTLRIFGNPGKDKVMIFPPTYG
                     MYEVSAAINDLEIIKVPLNENFQIDVNKAEQYLDDKNLKIIFICSPNNPTGNCMHSES
                     VEYIISRFEGIVLLDEAYSDFSDKPSFLRKTEKYPNLIVMQTFSKALGLAAIRIGMAF
                     ANPEIITYYNKIKPPYNVSTINQMAAIEKLSEGLNYRSQVKEIIRERERVAAEIRKLP
                     VTIKVFPSDANFLLVKVTDADMVYRKLISNDIIVRNRSSVVENCLRITIGKPYENDLL
                     LEALKNIQV"
     gene            complement(1303..2577)
                     /gene="hisD"
                     /locus_tag="PLG42_09605"
     CDS             complement(1303..2577)
                     /gene="hisD"
                     /locus_tag="PLG42_09605"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOU31503.1"
                     /translation="MKKYYYPARETWEALCERPSAGDDEEIRGIVADIIENVKRKGDS
                     ALEEYSMKFDGIKLTSLKVSDEEIERSAEAVPSSLKKSIDIAKRNIEKFHAALIHSDS
                     SVITSIGIKCWRRCVPIEKVGLYIPGGSAPLFSTVLMLGIPARLAGCREIILCSPPDK
                     EGNINPLILYTARLLDIRNIYRIGGAQAIAAMAFGTETIPGVYKIFGPGNRYVTKAKE
                     IVQEHGVAIDMPAGPSEVLVIAGKEARADYIAADLLSQAEHGPDSQTILVTDSADLID
                     AVESEIQKQLNLLPRKKIAEKAMSNSMAVLLNSLDDCIRFSNVYAPEHLIINTPDCHR
                     LAEKVSSAGSVFIGEFSCESMGDYASGPNHTLPTNGYARSFSGLSVDNFLKQIFFQEV
                     TRDGLATVGPAVIEMAEAEQLKAHSNAVKIRL"
     gene            complement(2577..3434)
                     /gene="hisG"
                     /locus_tag="PLG42_09610"
     CDS             complement(2577..3434)
                     /gene="hisG"
                     /locus_tag="PLG42_09610"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOU31504.1"
                     /translation="MNKLKIAIQKDGRLSDSSKKLLVECGLKFSNGTGMLKSVVTNFP
                     AEILYLRDDDIPQYVEQGVADAGIIGENMVAEKSKDIRIVERLGFARCRLSLAIPREE
                     EYTGPEFFNGKKIATSYPNILSDYLRKNNIKAAIEEISGSVEIAPGIGLADAVCDIVS
                     SGSTLMTNGLKEVVTILTSQAVLVVNKKLSGKKSEILDKLLFRIRAVKNAGENKYILL
                     NAPESAVARIAEILPGMKSPTILPLMEKGWVSLHSVVREDDFWESINMLKEAGAEGIL
                     VIPIEKMIK"
BASE COUNT          983 a          816 c          718 g         1234 t
ORIGIN      
        1 tcactgaacc ggattccttc accttcaaga atcttgagca tcttattgtg tgctggccag
       61 aaagtctctt caggaaaaag ctcagtacca agcccatcct gatttgtcac cataacaagt
      121 tcataatctg tttgtgttgc aatttttgca agagcagtaa ttaccccggg aacaaattca
      181 agtttttcaa gtgagtcgac ctgttcatca ggaggctctt ttatgatggt tccgtcgcgg
      241 tcgatgaaaa gagttctctt catacctgaa tatttttaag tgcttcgagc aacaggtcat
      301 tttcataagg cttgccaatg gttatcctca ggcagttttc aaccaccgac gacctgttcc
      361 ttacaattat atcattactg atgagtttcc tgtaaaccat atcagcatcg gtaactttta
      421 caagaagaaa atttgcatct gaaggaaata cctttattgt aacaggaagc ttcctgattt
      481 ctgcagcaac tctttcccgt tcacggatga tttcctttac ctgactacgg taattcaaac
      541 cttccgatag cttttcaata gctgccatct gattaattgt actcacatta tagggaggtt
      601 taatcttgtt gtaataggta attatttcag ggttggcgaa ggccatgcca attctgattg
      661 cggcaaggcc cagcgccttg ctgaaggtct gcattacaat aagattggga tatttttccg
      721 tttttcttag aaatgagggt ttatcactga agtcagaata tgcctcatcg agaagtacaa
      781 ttccttcaaa cctgcttatg atatactcaa cactttcaga gtgcatgcaa tttcctgtag
      841 gattattcgg agagcagatg aaaataattt taagattttt atcgtccaga tattgttcag
      901 ctttgttaac atctatctga aaattttcat ttagaggcac tttaataatt tccaggtcgt
      961 taatggcagc tgatacctcg tacatcccat aagtaggagg gaagatcatt actttgtctt
     1021 tacccgggtt accgaatatt ctgagtgtaa gatctattat ctcatcgctt ccgttaccaa
     1081 taaaaatata ttccttgttt attcctttaa gcctcgagac agctgatttc aggttttgct
     1141 ggtaaggatc gggataccgg tttagagttc cgtagggatt ttcgttagca tcgaggaaga
     1201 ctcccggctt accgttgtat tcatctcttg ccgaggaata gggtactaac ctttttatat
     1261 tttctcttac cagtttgtta atatcaggca tatatttagt ttttaaagtc tgatttttac
     1321 tgcattactg tgggctttaa gttgttcggc ttcagccatc tctattacag caggaccaac
     1381 agttgccagt ccgtctcttg taacttcctg aaagaaaatt tgttttagaa agttgtccac
     1441 cgacagcccg ctgaaactgc gagcatatcc gtttgtagga agtgtatggt tggggcctga
     1501 ggcatagtcg cccatgcttt cacaactgaa ttctccgatg aagactgaac ctgcagagga
     1561 gactttctca gcgagtctgt ggcagtcggg tgtgttaata atcagatgtt caggtgcata
     1621 tacgttactg aacctgatgc agtcgtcgag tgaatttaac agcactgcca tactgttgct
     1681 catagctttc tctgctatct tctttctggg caggagatta agctgttttt gaatctccga
     1741 ttctacagcg tcaataagat ctgctgagtc ggtgaccagg atagtctggc tgtctggtcc
     1801 atgttctgcc tgggaaagca ggtcggctgc tatataatca gccctggctt cttttcctgc
     1861 aatcaccagc acttcgctcg gaccggcagg catgtcaatt gctacgccat gctcctgtac
     1921 tatctccttg gcttttgtta cataccggtt gcctggtcca aatattttat aaacccctgg
     1981 aatcgtttca gttccgaaag ccattgcagc aattgcctga gctcctccga tcctgtatat
     2041 gttcctgatg tcaagtaatc tggctgtgta aagaatcagg gggttgatat tgccctcttt
     2101 gtcgggaggg gaacagagaa ttatttccct gcagcctgcc agacgggcag gaatacctag
     2161 cataagaacg gtagaaaaga gaggtgcact gccaccaggt atatacaaac ccaccttctc
     2221 aatgggaaca catctcctcc agcatttaat accgatggaa gtaattacgg atgagtcgga
     2281 atgaatcagg gctgcatgga atttttcaat attacgtttt gcaatatcta ttgatttttt
     2341 caaggatgag ggcacagctt cagccgacct ttctatttct tcatcggata cttttagaga
     2401 ggtaagcttt ataccatcaa acttcattga atattcttcc agagcagaat cgcctttgcg
     2461 ctttacattt tcaataatat cagctacaat tccgcgaatc tcttcatcat ctcccgcaga
     2521 aggcctttca cataatgctt cccatgtttc tcttgcagga tagtaatatt ttttcatcac
     2581 ttaatcattt tttctattgg tataacaaga attccttcgg cacctgcttc tttcagcata
     2641 ttaatgcttt cccagaaatc gtcttctcta acaacagaat ggaggctcac ccagcctttt
     2701 tccataagag gaagtattgt aggactcttc atccccggaa gtatttcagc aattctggca
     2761 acagctgact caggcgcatt gagaagtata tatttgtttt ctccagcatt ctttactgcc
     2821 ctgatcctga aaagcaattt gtccagtatc tcagacttct tcccgcttag ctttttgttt
     2881 actacaagca cggcctggct tgtaagtatt gttactactt ctttaagtcc gttggtcatg
     2941 agtgtactac ccgagctgac tatatcacac acagcatcag caagtcctat cccgggagct
     3001 atttcaacac ttccgctaat ctcctctatt gctgctttta tattgttttt tctgagataa
     3061 tcagaaagta tattaggata actggttgct atctttttgc cgttgaaaaa ttccggacca
     3121 gtatactctt cttctctcgg tattgcaaga cttaacctgc accgtgcgaa tcccaacctc
     3181 tcaacaattc ttatatcctt gctcttttcg gcaaccatgt tttctcctat aattcctgca
     3241 tcggcaaccc cctgttcgac atattgagga atgtcgtcgt cgcgcagata aagtatttcg
     3301 gctggaaaat ttgttacaac cgattttagc atacctgttc cattggaaaa tttcaaacca
     3361 cactcaacaa gcagtttctt tgagctgtca ctaagccttc cgtctttctg aatagctatt
     3421 ttaagcttgt tcatatttgt gtattattag ttattataag tcatgttaac cgtataaaat
     3481 caaaaaaacc cgccggaaca tcctcagacg ggtttatgat attttctgca tcagcaacaa
     3541 catcactgcc tcgcctgagg tgagctatga tgatgatgct gatatgtacg aatcctgttc
     3601 atttttataa ttcagaggtc aaaaatatat tttttttaat taaaatgcaa gattagtgaa
     3661 atttatttac ttggtgtgat atattttagt tctgacaaga tgatttttcc cgtaaaaaat
     3721 taaaattgta accgatagaa tcaacagaat c
//
LOCUS       DAOSNB010000265         3710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_24905_length_3710_cov_3.429549, whole genome shotgun sequence.
ACCESSION   DAOSNB010000265 DAOSNB010000000
VERSION     DAOSNB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24905_length_3710_cov_3.429549"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1143)
                     /locus_tag="PLG42_09615"
     CDS             complement(28..1143)
                     /locus_tag="PLG42_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU31505.1"
                     /translation="MNRKVTIRKCDSYNLEEVIRVVADVYENSEGPDLMGKRVLVKPN
                     ILTDSPPEKSICTHPVVVEAVIRYIQSKGAEVVVGDSPSVHIGNFRPVKCGIEDVCRR
                     TGVQWVDFKEKPVEVNLRNGKKIKVASVIRDVDLIISLPKFKNHELAYFTGAIKNTLG
                     VVPGFIKGKQHALYQNRMAFSRFLVDLNEAVLPDYFLMDAIYGMEGPGPANGFPKKIG
                     LLIGSANPLALDIIASTIAGYKPIDIPTNRIGLSRGEWLTSVDEIVYDGPSLDSLVLK
                     DFLKVPVTRMENISLQFLIRRVKFLRKLERRPVFLHDRCTGCLKCVKICPVNAISPHP
                     SKKNHIVLTDKKCIRCYCCSEVCTDNAIEIRRKLFGV"
     gene            complement(1200..2642)
                     /gene="dacB"
                     /locus_tag="PLG42_09620"
     CDS             complement(1200..2642)
                     /gene="dacB"
                     /locus_tag="PLG42_09620"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOU31506.1"
                     /translation="MNKLISTTILFLALITIKSESQTAALERFLSDSLMQHASISFLI
                     KDAVTGDTLAEYDSNRSLTPASVLKLITTAAALELLGPDYRFRTTLEYSGTLLKNSGV
                     LKGDIIIKGGGDPCLASERFTEHYGNLFNDWAAAVRSSGIKKIKGRVITDDSYFDYEP
                     VPDGWVWEDIGNYYGAGVYGLSVFDNTSKIHFRTYEEGSAPEIIMIEPFEAGNEYISN
                     LIASGTTDRGYVFCSPYGNKGWISGTIPPNREDFILKAAITDPPLVAAKILTGKLIKK
                     GIKVSGNPSTYRLKKQIKNQNNTIINETLSPPLNEIIEVLNHESINLYAEHLVKELGK
                     AFRENGSASAGLEVINDFLKKNGIYNPGMVIIDGSGLSPRNLVNASGIVSLLLYMKNS
                     NNYAHFINSLPEAGAEGTLKNVFRGPEFEGRLNAKSGSMAGIRSYAGYLKSVSGKELV
                     FCIIVNNYSGSSGNVTRYIEEILKEAVVNN"
     gene            complement(2639..3670)
                     /gene="rlmN"
                     /locus_tag="PLG42_09625"
     CDS             complement(2639..3670)
                     /gene="rlmN"
                     /locus_tag="PLG42_09625"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOU31507.1"
                     /translation="MKKEPLYGKTINELIAITRRIGLPGYSARQIADWLYKHQISSVE
                     EMTNLSKKARDMLSSEFEYGLSSPAEYLESSDGTKKYLYKVLDDKYIETAFIPEKERA
                     TICVSTQAGCKMGCLFCMTGKQGFQGNLSSNEILNQFRSLPEFSRITNIVYMGMGEPL
                     DNIEEVLKSLEILTSDWGYAWSPTRITVSTVGIVEKMTAFLERSRVHLAVSLHTPFDE
                     ERLMLMPVQRTNPVREVIEIIRNAGITGQRRVSFEYILFKGFNDTPAHIKELSRLLNG
                     IRCRVNLIRFHKIPGVSFESPGIEEVKKFRDMLNKKGIVATIRASRGEDIKAACGLLS
                     TLSRNKTRS"
BASE COUNT         1041 a          833 c          666 g         1170 t
ORIGIN      
        1 gcaagaatag atgcataaag gggaaagcta tacaccaaaa agcttcctcc ttatttctat
       61 tgcattatca gtgcatacct cactgcaaca gtaacatctg atacattttt tgtcggtcaa
      121 cacaatgtga ttcttctttg acggatgcgg ggaaattgca ttcacaggac agattttcac
      181 acatttcaga catccggtgc atctgtcatg caaaaaaacc ggccttcttt caagcttcct
      241 cagaaacttc accctcctta tgagaaactg cagggaaatg ttttccatcc gggtcacagg
      301 tactttcaaa aagtctttta atacaagcga atcaagagaa ggcccatcgt aaacaatttc
      361 atcaactgat gtaagccatt ctcctcttga tagtccaatc cggttggttg gtatatcaat
      421 gggtttatag cccgcaatag ttgaggcaat tatatcaagt gcaagcggat tggctgatcc
      481 aatcaaaaga ccaatctttt tcggaaaacc atttgcaggc cccggacctt ccatcccgta
      541 aatggcatcc ataagaaaat aatccggtag cactgcttca ttcaggtcaa caagaaacct
      601 gctgaatgcc attctgttct gataaagtgc atgctgcttg ccttttataa acccgggaac
      661 aacgcccaga gtgttcttta tagctcctgt gaaataagca agttcatgat tcttaaattt
      721 tggaagagaa attataagat caacatccct tattactgag gcaactttta tcttttttcc
      781 attccggaga tttacctcaa caggcttttc cttgaaatca acccattgca cgccagtgcg
      841 cctgcataca tcttcaattc cgcatttcac aggacgaaaa ttaccaatat gaaccgaagg
      901 tgaatcacca accaccactt cagcaccttt cgattgtata tacctgatta cagcttccac
      961 caccacagga tgagtgcaaa tgcttttctc aggcggagag tcagtcaaaa tgttcggctt
     1021 aacaagaacc ctttttccca taagatcagg tccttcagaa ttttcgtaaa catctgccac
     1081 aaccctgata acttcttcta aattataact atcacacttt cttattgtta ctttcctgtt
     1141 catttaaagt tcgtttataa aaaacagttt ggtttttgca gtcaaaagaa aaaattttat
     1201 taattattca caaccgcctc cttcaatatc tcttcaatat atcttgtgac atttcccgaa
     1261 gaacctgaat aattgttaac tataatacag aaaaccagct cttttcctga aacactttta
     1321 agataaccgg cataacttct tattccagcc atggaaccac tttttgcgtt tagccttcct
     1381 tcaaactccg gacccctgaa aacattcttc aaagttcctt cagcacctgc ttctggcagt
     1441 gaatttataa agtgagcata attattgctg tttttcatat atagaagcaa tgaaacaatg
     1501 ccgcttgcat taacaagatt acggggcgaa agaccgcttc cgtcaataat aaccatgcca
     1561 ggattatata taccgttttt tttcaggaaa tcgtttatca cctcaagacc ggcactggct
     1621 gaaccatttt ctctgaatgc ttttccaagc tcttttacca ggtgttcggc atacagattg
     1681 atactttcgt gattcaatac ttcgattatc tcattcaaag gtggcgaaag agtttcattt
     1741 attattgtat tgttttgatt tttaatttgt tttttaagcc tgtatgtgga aggattaccc
     1801 gaaactttta tacctttttt tataagtttc cccgtaagta tttttgctgc aacaagagga
     1861 ggatctgtta tagctgcttt tagaatgaaa tcttcacggt taggcggaat agtcccagaa
     1921 atccagcctt tgtttccgta tggagaacag aaaacatatc cccggtcggt tgtccctgat
     1981 gcaatgaggt tgctaatgta ttcattacca gcctcaaatg gttcaatcat tataatttca
     2041 ggtgctgaac cttcttcata cgttctgaaa tgtatttttg aagtgttgtc aaataccgac
     2101 aggccatata ctcctgcgcc ataatagttg ccgatatctt cccacaccca gccatcaggt
     2161 accggttcat aatcaaaata tgaatcgtcg gtgataaccc ttccttttat ttttttaatc
     2221 cccgaactcc tgactgctgc cgcccagtca ttaaaaagat ttccataatg ttcagtaaat
     2281 cgttcggatg ccagacaggg atcacctccg ccttttataa ttatatctcc cttaaggacc
     2341 cctgaatttt tcaaaagagt tcctgaatac tcaagtgtgg ttctgaacct gtagtcggga
     2401 ccgagcagtt caagagccgc agcagtggtt atcagcttca aaacagacgc aggagtcagg
     2461 cttctgttac tgtcatattc ggctaaagta tcacccgtta cggcatcttt tattaaaaat
     2521 gaaatggatg catgctgcat cagtgagtcg gaaagaaacc tttccagagc agcagtctgt
     2581 gattctgatt ttatggtgat taacgcaaga aagagtattg ttgttgaaat cagcttgttc
     2641 atgacctggt tttatttctt gaaagggtgg aaagaagtcc gcatgccgcc ttgatatcct
     2701 ctccacgact ggctcttata gtggcaacaa tacctttttt attgagcata tctctgaatt
     2761 tttttacttc ttcaataccg ggactttcga agctgacgcc gggaatttta tgaaacctta
     2821 taaggttaac acggcatcgt attccgttaa ggagtctgga gagctcttta atatgtgccg
     2881 gtgtatcatt aaagcctttg aaaaggatat actcgaatga gacacgtctc tgtcctgtta
     2941 ttcctgcatt tcttataatt tcaatgactt ccctgacagg atttgttctt tgaaccggca
     3001 ttagcataag tctttcctca tcgaatggtg tatgaagact tactgccaga tgaactctgc
     3061 ttctttcaag aaaggcagtc atcttttcta caatgccaac tgtactgaca gtgatgcgcg
     3121 ttgggctcca tgcatacccc cagtcggatg taagtatttc cagacttttc aaaacctctt
     3181 cgatgttatc aagaggttcg cccataccca tatagactat attcgtgatc cttgagaact
     3241 ccggaaggct tctgaactgg ttgagtatct cattcgacga caggttaccc tgaaaaccct
     3301 gttttccagt catacagaaa aggcatccca ttttgcatcc tgcctgggtt gaaacgcata
     3361 tcgttgcacg ctccttttcc ggtataaaag ctgtttctat atatttgtca tccaatactt
     3421 tgtatagata tttctttgtc ccgtcagaac tttcaaggta ttcggcagga gaggagagcc
     3481 cgtattcaaa ttctgaagaa agcatatccc ttgccttctt tgagaggttt gtcatctcct
     3541 cgacagaaga aatttgatgt ttatacagcc agtcagctat ctgacgagcc gaataacctg
     3601 gcaaaccaat acgtcgtgta atggcaatca gctcattaat tgtctttccg tataacggtt
     3661 cttttttcat aagctatata atataatcta tattccctgt gccctgagct
//
LOCUS       DAOSNB010000266         3659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_25368_length_3659_cov_2.657603, whole genome shotgun sequence.
ACCESSION   DAOSNB010000266 DAOSNB010000000
VERSION     DAOSNB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25368_length_3659_cov_2.657603"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PLG42_09630"
     CDS             <1..302
                     /locus_tag="PLG42_09630"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOU31508.1"
                     /translation="CAYSFVDDGILKCAIEKAWEEGKISFRKPLSCHLFPVRVTRYSD
                     FTAINVEEWPICFAGREKGRKEGIALYEFLEGALTRLAGEEVYREICNSAKHIRP"
     gene            461..3568
                     /locus_tag="PLG42_09635"
     CDS             461..3568
                     /locus_tag="PLG42_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOU31509.1"
                     /translation="MKKLSLLIGLLSLSVITLFSQQTRVITGTVTSAVEGEGALPGVS
                     VTVPGTTIGTYTDINGKFTLNVPANARTLVFTFIGMKVKEVDIAGKNSVDVLMEQDVM
                     NLDEIIVTGVAAGTPRKKMAVAVEKVDADKLKEVTAGSASTALQGKVAGLTVVNPSGA
                     PGRSSTILVRGATQISGSQDPLILLDGAIMEGTLADINVDDIESIEVVKGASAAALYG
                     SRAGNGIIAVTTRRGSNLGEGRTEVIFRNEYGLNQLAKKYDLATHHAFVLANDYENYS
                     YTKYAGVTYPAGYHASEGGITGSRYYESDHYMDNPYMTLYDHEGDMFAGNNFYTNYLS
                     VGSNLGKTNFLVSFENNVQSGLLVETTGYKRNSFRLNVDHKISPKLSVSASNLYVRST
                     TQDPGGENAYNGGIFFNILLLEPDADLKFPNPDGQPYLFIPNVWQAEQENPLYNVWKL
                     EDLNIRNRILGTYQLKYNMTSSLNFEAKYAFEYTPATYRSYSPYDTYTRSGGQAVYSQ
                     GYLQISSSTLFSRNAQITANYSKKFGDFNVRAKASYLFEKSTYESTTTTGYDFSLKGV
                     PSFDAISGNKSITSYMNAILAKNYFGILYLDYKDRYIFDGMVRYDGSSLFGADARWNP
                     YYRVSGAYRLSEDLKLPGIQELKLRAAYGTAGQRPGFADQYEVMQISGGNVSKLQLGN
                     SKLKPSLSKEMEFGLNIDFLNRFSFEISRSETNTSDQIITVPLAVQYGGWPSQVQNAG
                     SLNSKVWEASLNTQVVKTKGINYNINIIFDRIRTKITQLDVPPFQTGPQGQEANKCFY
                     IREGETLGAMYGYKFLKSLAEMEEQLQPGDNIDNYTVNSDGYVIVKGTEGTVSEAPIK
                     KLDENGKLWYGKIGDSNPKFKIGMTHTFTYKGFGLYALLEWKNGGDIYNKSAQWLTRD
                     DRHGMMDQYGKPDYLKKTIEYYKVFYDVNDFNDFWVEDGSYLKLREASLSYTFPASLF
                     KNFANNLIKGVKVSVIGKNLLTVTNYTGYDPEVMTTNNVQYFSYDFMGYPNYRSYSAS
                     IEIKF"
BASE COUNT         1133 a          713 c          798 g         1015 t
ORIGIN      
        1 agtgtgccta ttcgttcgtt gatgacggca ttctcaaatg tgcgattgaa aaggcatggg
       61 aggagggaaa gatttcattc agaaaaccac tctcatgtca tcttttccct gtcagggtga
      121 cgcgatacag tgattttaca gcaataaatg tggaagagtg gccgatctgt tttgccggga
      181 gggaaaaggg aagaaaggaa ggtattgccc tttatgagtt tcttgaggga gctctgacca
      241 ggctggcggg agaggaggtt tacagggaaa tttgcaattc ggcgaagcat atccggccct
      301 gagagaggtt aataatatta atctttactg aaaccggcaa tttccggaag gatatcgtgc
      361 cattcttcaa acataaaact tttcaatgta agaatttttc gtttcaacag aatattttgt
      421 atatttgttc caaattagct aaaccttaaa ctaaatcatt atgaagaaat tgtctttgtt
      481 gattggttta ctgtcattaa gtgttatcac acttttttct cagcaaacac gcgttattac
      541 gggtacggtt acctctgcgg ttgaaggcga aggggctcta cccggtgtat ctgtcacggt
      601 gccaggcaca actatcggca cctatacgga tattaatggt aaattcacac tgaatgtacc
      661 tgcaaatgcc agaactcttg tttttacatt tattggtatg aaggtcaagg aagtggatat
      721 tgcaggaaag aattcagtag atgtgttgat ggaacaggac gtcatgaacc tggatgaaat
      781 tattgttaca ggtgtcgcag ccggaacccc acgtaaaaaa atggctgttg cggttgaaaa
      841 agttgatgcc gacaaactga aagaagttac agcaggttct gcttccacag ctcttcaggg
      901 taaggttgcc ggacttacag ttgtaaatcc atcaggtgca cctggccggt catcaacaat
      961 acttgtccgt ggagccacac agatttcagg ttcgcaggat cctctgatat tgcttgacgg
     1021 tgcaattatg gagggaacgc ttgcggacat aaatgttgat gatatagaat caattgaagt
     1081 tgttaaaggt gcctctgctg cagcacttta cggttcccgg gcaggaaacg gaattattgc
     1141 cgtaacaaca aggcgcggat ctaacctggg agaaggaaga accgaagtga tattcagaaa
     1201 cgaatatggg cttaaccaac ttgcaaaaaa atatgatctt gctactcatc atgcatttgt
     1261 cctggcaaat gactacgaaa attattcgta tacaaaatat gccggtgtaa catacccggc
     1321 aggttatcat gccagcgagg gtggaattac cggatcgcgt tattatgaat cagaccacta
     1381 catggataat ccttacatga ccttatacga tcatgaagga gatatgttcg ccgggaataa
     1441 tttctataca aattatcttt cagtaggatc aaatctgggg aaaaccaatt ttctggtttc
     1501 cttcgaaaat aacgtgcagt ccgggttgct tgttgaaaca acaggttaca agagaaacag
     1561 cttcagactg aatgttgacc ataagatttc gccgaaactt tcagtatcag caagtaacct
     1621 ttatgtaaga tcaacaacac aggaccctgg aggtgaaaat gcttataacg gcggaatttt
     1681 cttcaatatt ctgcttcttg aacctgatgc agatcttaaa ttccccaacc ctgacggaca
     1741 gccttatctt ttcattccga atgtatggca ggccgaacag gaaaacccgc tttacaatgt
     1801 ctggaaactt gaggatctga atataagaaa caggatactt gggacgtacc agttaaaata
     1861 caatatgaca agttccctga acttcgaagc aaaatatgct tttgaatata ctcccgctac
     1921 ttaccgtagt tattctcctt atgacactta cacaaggtct ggcggacagg ctgtatattc
     1981 gcagggatat cttcagattt ccagttcaac tcttttctca agaaacgcgc agattactgc
     2041 caattacagc aaaaaatttg gtgacttcaa cgtaagagca aaggcaagct acctttttga
     2101 aaaatccact tacgaaagca ctacaactac cggttatgat ttctcgttaa aaggggtgcc
     2161 atcttttgat gccatttcag gaaacaaatc cattacaagt tatatgaatg ccattcttgc
     2221 caaaaactat ttcggcatac tttatcttga ttacaaagac cgttacatct ttgatggcat
     2281 ggtacggtac gacggctcct cactttttgg tgcagatgca agatggaacc cctattacag
     2341 agtgtcgggt gcttaccgtc tttcagagga tctgaaatta ccgggtatac aggagcttaa
     2401 actcagagca gcctacggaa ctgcaggaca aagaccgggt tttgccgatc agtatgaagt
     2461 aatgcagatt tcaggtggaa atgtttcaaa actgcaactt ggtaactcaa aacttaaacc
     2521 ttctctttca aaagagatgg agttcggtct taacatcgat ttcctgaacc gtttcagttt
     2581 tgaaatatcc aggtctgaaa caaatacttc agatcagatt ataaccgtac cactggcagt
     2641 tcagtacggc ggatggccaa gccaggtaca gaatgccggg tcccttaatt caaaggtgtg
     2701 ggaggcatca ctgaatacac aggttgttaa aacaaaagga attaattata acataaacat
     2761 tattttcgat cgcatccgga caaaaattac tcagctcgat gttcctccat tccagaccgg
     2821 accgcaggga caggaagcaa acaagtgttt ctatatacgt gaaggagaaa ctcttggggc
     2881 tatgtacggt tacaaattcc tgaaaagcct tgctgaaatg gaagaacagc ttcagccagg
     2941 cgacaatatc gacaattata ccgtaaacag cgatggttat gtgatcgtaa aaggcactga
     3001 aggaactgtt tcggaagcac ccatcaaaaa acttgatgaa aacggaaagc tctggtacgg
     3061 aaagatcgga gattcaaatc caaagttcaa gattggaatg acccatacat ttacctataa
     3121 gggattcggt ctttatgctc ttctggaatg gaaaaacgga ggcgatattt acaataaatc
     3181 cgcacagtgg cttacgagag atgacagaca tgggatgatg gatcagtacg gtaaacctga
     3241 ttacctgaaa aaaacaatcg aatattacaa ggtcttttat gatgtaaatg atttcaatga
     3301 tttctgggtt gaggatggaa gttatttaaa gctcagggag gcttcactct cctatacatt
     3361 cccggcttct ctctttaaaa actttgccaa taacctgata aagggagtta aggtttcagt
     3421 aatcggcaaa aacctgttga ctgtaacaaa ttacacaggt tacgatccgg aagttatgac
     3481 taccaataac gttcagtatt tctcatatga ttttatggga tatcccaatt acaggtcata
     3541 ctcagcttca attgaaatta aattctaata cgaaaattat tgtgctatga agacaattaa
     3601 atattttctg atattttctg ttattgcggc tttcatcctt gcaggatgtg aaagcctgg
//
LOCUS       DAOSNB010000267         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_25386_length_3656_cov_3.498195, whole genome shotgun sequence.
ACCESSION   DAOSNB010000267 DAOSNB010000000
VERSION     DAOSNB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25386_length_3656_cov_3.498195"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="PLG42_09640"
     CDS             complement(<1..1737)
                     /locus_tag="PLG42_09640"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOU31510.1"
                     /translation="MIFGGNIIPKGKINILLILLFIPLFNTEAQVIFHRPLSQRLTGY
                     RIDAVLDPLSKVVKGKMEAFWVNNSSDLVPDVRLHMYLNAFSSNRTTFYRERGGSPGS
                     REEDLGWIKLNSIKDRNGIDLKEVAKYISPDDGNPDDRTVLQIILPRPANPGDTVYLI
                     VEFESKLPSNIIRTGYSDDFFFVAQWFPKFGVYEPAGMRYAATGGWNCHQFHGNSEFY
                     SNHSVYDVTITVPENYVVGTGGMTLEEKNNGDGTKTLVTRAEDIVDYAWTAWPGYAVF
                     KDKWDHVDITFLTPPERKNMVKRHLTAIKYALEYLTENVGPFPWPHLTFVDPPSKGSG
                     AGGMEYTTLFTTMSAYGLPEYVLMPEMVTVHEFGHAYFMGILASNEFEEPWLDEGVNS
                     YWEERIMDHYYGNGAGMINHPLLKISDRSVARVSYVMSPSRQAESNAQWSWNYPHGTY
                     SMLSYQKCALWLQTLTGLVGEEAMNEAFREYYRQWAFKHPSGQDFVNVFNTVIPKIYG
                     NRYGSNLNWFFDQTLYGTGICDYKVVNITNRKVRDYEGAYFENDSMEVKKGNYKDDTL
                     WVSAVQLERNGEL"
     gene            complement(1734..2114)
                     /locus_tag="PLG42_09645"
     CDS             complement(1734..2114)
                     /locus_tag="PLG42_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31511.1"
                     /translation="MKKVFSLLVFFIVFSIAGFSQASKQKLEPAGKWQFEAPYAPEGY
                     TSGIMEFVKAEGKYQGFVSFTGSDYKIPLDRVSVKSDSILVSLYVEGADVPIKLKMVD
                     PEKITGVASSPDGDIPLTATRAKQ"
     gene            2498..>3656
                     /locus_tag="PLG42_09650"
     CDS             2498..>3656
                     /locus_tag="PLG42_09650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOU31512.1"
                     /translation="MKKILILGAGTAGTIMANKLRKELPREEWKITIVDQEKVHYYQP
                     GFLFIPFGYYKKADVTKPKQSFIPPGVDIYFRKIEQVIPGENKVRLDNGEVLLYDILI
                     IATGTRVSPEETPGLKGELWHKNIFDFYTIEGAQALANFFKTWEGGDLVIDIADDPIK
                     CPVAPLEFAFYADAFFTERGMRNKVNIFYVTPQSGAFTKPRSSKLLGNMLEDKNIKMV
                     NDFVLSEVDNKRKVITDYSGREVHFDCLVSIPLHIGDPGLNDSGLVDEFGFVRADKYT
                     LQSVDFENIFVIGDASNLPTSKAGSVAHFQSEILLENLICYIEGRPFTAKYDGHSNCY
                     IETGYGKAALIDFNYDTEPLPGFFPFPGIGPFSLLKESRINHYGKLIFRWMY"
BASE COUNT         1056 a          769 c          689 g         1142 t
ORIGIN      
        1 taattctccg ttacgctcaa gctgcactgc tgaaacccag agggtatcat ctttataatt
       61 acccttttta acctccatag aatcattctc aaaatatgct ccttcataat ctctcacttt
      121 tctgttggtt atattgacaa ctttataatc gcagatacct gttccataaa gtgtctggtc
      181 aaagaaccag ttaaggttgc ttccatatct attaccgtat attttgggga taaccgtatt
      241 aaatacgttg acaaaatcct ggcctgaagg gtgtttgaag gcccactgcc tgtaatattc
      301 tctgaaggct tcgttcattg cttcttcacc cacaagtccg gtgagtgttt gaagccatag
      361 agcacacttc tggtatgaga gcatgctgta agttccgtgg gggtagttcc acgaccattg
      421 tgcattgctt tcggcctgcc tcgatggaga catcacgtat gatactctcg ccactgaacg
      481 gtcggatatt ttgagaagag gatggttaat catacctgcc ccgttaccgt aataatggtc
      541 cattatgcgc tcttcccagt aggagttaac cccttcatcg agccatggtt cctcgaactc
      601 gttactggca agaatgccca tgaaataggc atgtccaaac tcatgtacag taaccatttc
      661 gggcataagt acatattcgg gcaatccata tgccgacata gtggtgaaca atgttgtata
      721 ctccattcct ccggcgcctg atccttttga cggcggatct acaaatgtga gatgcggcca
      781 tgggaaagga cccacgtttt cagtcagata ttcaagggca tattttatcg ctgttaaatg
      841 cctcttaacc atattctttc tttcaggtgg cgtcaggaat gttatatcta catgatccca
      901 tttatcttta aatactgcat atcccggcca tgcagtccat gcataatcta ctatatcctc
      961 agctcttgta actaatgttt tggtaccatc tccgttattc ttttcctcaa gcgtcattcc
     1021 tcctgttccc actacgtaat tttcaggcac tgtaatagtt acatcataaa cactgtgatt
     1081 tgaataaaat tcagaatttc catgaaactg gtggcagttc catccgcctg ttgcagcata
     1141 tctcatcccg gcaggttcat agacaccaaa ttttggaaac cattgcgcca caaagaagaa
     1201 atcatcagaa tatccggttc tgataatgtt tgaaggcagc ttcgattcga attctacgat
     1261 aagatatact gtatctcccg ggttagcagg tctgggaaga attatctgca gtactgttct
     1321 gtcatcggga ttaccgtcat cgggacttat gtacttcgcg acttctttca ggtcgatacc
     1381 gtttctatcc ttaattgaat tcagttttat ccatcctaaa tcttcctccc tgctacccgg
     1441 ggaccctcct ctttccctgt aaaaagttgt acggttactg ctgaatgcat tcagatacat
     1501 gtgcagtctt acatctggaa ctaaatctga tgagttgtta acccagaagg cttccatctt
     1561 cccttttacg acctttgata aagggtccag aacggcgtcg attctgtaac ctgtaagcct
     1621 ctggctcagg ggacggtgaa atattacctg tgcctctgta ttaaacagtg gaataaaaag
     1681 gagaattaat aatatgttga tttttccttt cgggatgata ttcccgccga aaatcattgt
     1741 ttggcccttg ttgccgttaa aggtatatct ccgtcgggtg atgatgcaac acctgttatt
     1801 ttttccggat caaccatctt cagtttaata ggaacatcag ctccctcaac ataaaggctt
     1861 accagaatgg aatcactttt cacagaaacc ctgtcgagag gaattttata atcactaccc
     1921 gtaaatgaca caaatccctg gtatttgcct tcagccttta caaattccat tatacccgaa
     1981 gtataaccct caggagcata aggggcttca aactgccatt taccggcagg ttccagtttc
     2041 tgtttggagg cctgtgaaaa tcctgctatc gaaaaaacaa taaaaaagac cagtaatgaa
     2101 aagacttttt tcataagcat ttattttaat attgggttgt ctttcaataa tttcatcatt
     2161 ctgctattat tatgaatctt acatatccta accccatggt gaacaatcta tagcctggga
     2221 ttgttttctt atatatttcg aagttttaat aattgtctaa aaatataaaa ttgatttcaa
     2281 ataaaatcta aatgataaaa aaatttatct gttggtttat ttgtgagtgt tcctatttat
     2341 gtaaaattag gccttcatta aattcaacct tttcctgttt tcatacgtaa agaattaata
     2401 taattatatc tttgcactgt taattaatta acaataagtt gtctaaacac tataaaccat
     2461 aattatctga ataattgcat aatacaaaga ttatccgatg aaaaaaattc taattcttgg
     2521 ggccggtaca gccggaacca taatggcaaa caaattaagg aaagaacttc cccgcgaaga
     2581 gtggaaaatt accattgttg accaggaaaa ggtacattat tatcaacccg gctttttgtt
     2641 tattcccttc ggatattaca aaaaagcaga tgtaacaaag cccaaacaaa gtttcattcc
     2701 accgggtgtt gacatatatt tcagaaaaat agagcaggtt attcctggtg agaataaagt
     2761 aagactggat aatggcgagg tactccttta tgatatactc ataattgcta caggaacaag
     2821 agtctccccc gaagagactc ccgggctgaa aggtgaactc tggcataaaa atatttttga
     2881 tttttacaca attgaagggg cacaggcatt agcaaacttt ttcaagacat gggagggagg
     2941 agacctggtt attgacatag ccgacgatcc gataaaatgc cccgttgcgc cccttgaatt
     3001 tgcattttat gctgatgcct tctttactga aaggggtatg agaaacaagg taaacatttt
     3061 ttatgtgacg cctcaatctg gtgcatttac caagcccagg tcatcaaaac tcctgggaaa
     3121 tatgcttgaa gataaaaata ttaagatggt taacgatttt gtactcagtg aggttgacaa
     3181 taaacgcaaa gtaataacag attattctgg cagggaagtt cattttgact gcctggtatc
     3241 cattccgctt catatcggtg atcctggcct taatgattcc ggacttgttg acgaatttgg
     3301 atttgtcaga gctgataagt ataccctgca atcggtagat tttgaaaata tatttgttat
     3361 tggcgatgca agtaacctgc ctacttccaa ggccgggtcg gttgctcatt ttcagtcaga
     3421 aatacttctt gaaaatctta tatgttatat tgaaggaaga ccctttacag ctaaatatga
     3481 cggccattcc aactgctata ttgaaacagg gtatggcaag gctgctctta tagattttaa
     3541 ctacgacact gaacctcttc cgggcttctt ccctttcccg ggcattggac cattcagttt
     3601 attgaaagaa agcagaataa accactatgg taaactcatc ttcagatgga tgtact
//
LOCUS       DAOSNB010000268         3649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_25452_length_3649_cov_4.108514, whole genome shotgun sequence.
ACCESSION   DAOSNB010000268 DAOSNB010000000
VERSION     DAOSNB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25452_length_3649_cov_4.108514"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLG42_09655"
     CDS             complement(<1..1122)
                     /locus_tag="PLG42_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31513.1"
                     /translation="MKTSLRNLITLMILATSLSGCMDKTIERVTYTVNVPIYMSYSEF
                     RSSFKTSEPKEISQPGKIYFKDNFLFVNELQKGIHVIDNSDPAHPKKVAFYDIIGNVD
                     MAIKGNILYADSYIDLVAIDISDISKPKEVGRLKNIFPEVVPVGDRWYPFVDVDKSKG
                     VIVGWEVKTVTEEVYNNSDWGLKIYRGDLVFAAMAYEGGGNWSSGAGTGGSMARFMLN
                     DDNLYVIAHTWMLKTVDIKMPDKMSVVDSIAVPRTMETLFKLGEKLFVGTTSGMLIYD
                     ISNPVKPVQISVYNHITACDPVVADNKYAYVTLRTGTRCANSQNLLDVIDISSITNPY
                     LVKSYPLFNPHGLGIDGNLLFICDGKAGLKIYDKSDPLAI"
     gene            complement(1255..2493)
                     /locus_tag="PLG42_09660"
     CDS             complement(1255..2493)
                     /locus_tag="PLG42_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOU31514.1"
                     /translation="MRTKALTAAGLLAIFFLLVSCEKQPGEPDLTPAKIELTPAASQV
                     ISSGNEFGVDLFTKVAETEDRNLMLSPLSASTALTMLLNGCSGNTFTQLRSTLKYPSG
                     MQTEDINEAYKSLVEQLLKADPKVTFALANAIFYRLGFNVKAQFINSMKENFKAKIEG
                     LDFNAPSALNTINKWASDNTSGKIPKVLDQISDETVMFIMNALYFKGDWSYKFDKSKT
                     TERQFYPDNLPAVNVPTMNGEIGAKVVYREKFRAIEMPYGRTNFTMIVIVPEGGLKSF
                     NSSFDSDLWKSLTSELDEIEEFGKCIVYFPKFKFSYEKILNDQLAAMGMVDAFIPYVA
                     DLSGISDAEIFVSFVKQNTFVEVDEEGTEAAAVTTIGMELTSMPPQPQQFVIDRSFIF
                     AIRERTTNTLLFIGQVVNPE"
     gene            complement(2593..2877)
                     /locus_tag="PLG42_09665"
     CDS             complement(2593..2877)
                     /locus_tag="PLG42_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31515.1"
                     /translation="MPLPASASGEERDGMHTISSFPQFGEMVEEGERQLKASALKVWV
                     NARRLKKTLTKLSNFFTVIFEPLTNFRRSYNSESNIPSSFFRINGCHRIG"
     gene            complement(2888..>3649)
                     /locus_tag="PLG42_09670"
     CDS             complement(2888..>3649)
                     /locus_tag="PLG42_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31516.1"
                     /translation="CEPLKNDPLLIGYFVGNEPAWDGRESEVVDMILAGPDNEIKKRL
                     KEFLSAGDTPGRRREFVLGAFEKYLQLICSAIKKYDPNHLNLGIRFGGSPSAGLLKTG
                     RIFDVCSINVYEYEPVAQIERVYRYTGRPVLIGEFHIGVPENGLGAGLVQAMNQKERG
                     IAYRYYVEQAAATAAFVGAYWFQWRDQPVLGRFDGENYNIGFVDVTDRPYKELVEAAK
                     ETHKRLYDVHAGKILPFSQRPKASPAGTPRSPWDF"
BASE COUNT          951 a          876 c          700 g         1122 t
ORIGIN      
        1 tattgccagc ggatcagatt tgtcgtatat cttcagaccc gccttaccgt cacaaataaa
       61 cagcaggttg ccgtctattc cgagtccgtg aggattaaaa agtggatatg actttaccag
      121 gtacggattg gttattgatg aaatatcaat aacatcgagc agattttggc tgttggcaca
      181 tcttgtaccg gtacgcaatg tcacatacgc atatttatta tctgccacca ccgggtcaca
      241 ggcagtaata tggttgtaaa ctgaaatctg tacgggttta accggattgc ttatgtcgta
      301 aatcagcata cccgaggtgg tacccacaaa aagtttttca cccagtttga aaagtgtttc
      361 cattgtacgt ggaactgcaa tgctgtcgac cacgctcatt ttatcgggca ttttgatatc
      421 cactgttttg agcatccagg tgtgggcaat cacataaagg ttatcgtcat taagcatgaa
      481 ccttgccatt gagcccccgg ttccagcacc gctgctccag ttaccgcctc cttcatatgc
      541 cattgcggca aatacaagat cccccctgta aatcttcagt ccccaatcgg aattattata
      601 tacctcctca gttactgttt tgacttccca gcctactata accccttttg acttatcaac
      661 atccacaaac ggataccatc tgtcaccaac aggaacaact tcagggaaaa tatttttcag
      721 gcggccaacc tctttgggct tgcttatgtc agagatgtct attgccacaa ggtcgatata
      781 gctgtcggca taaaggatgt tgccttttat tgccatatcc acattaccta tgatatcata
      841 aaaagccacc tttttcggat gtgccggatc agaattgtca ataacatgaa tccctttctg
      901 taattcgttt acaaacagaa aattatcttt aaagtaaatt ttccccggct gactgatttc
      961 ttttggctcg cttgttttga agctgctccg gaactctgaa taagacatat atatcggcac
     1021 attcaccgtg tatgtcactc tttcaatggt cttgtccatg caacccgaca atgaggttgc
     1081 aagaatcatt aatgtaatta agttcctaag tgaagttttc ataatatgtt atttacatgt
     1141 tttccagttt tttgaatatt tttcagatgc ttattttttg tactaaacta taaaacaccc
     1201 actttatgct ttgctctcat ccctcactcc tcactcctca ctcctcaccc ctcattactc
     1261 cggattaacc acctggccta taaacaacag ggtattcgtg gtacgttctc tgattgcaaa
     1321 gatgaacgac ctgtctatca caaactgttg tggctggggc ggcatacttg ttaactccat
     1381 gcctattgtt gtcactgcag ctgcttcggt tccctcttca tcaacttcaa caaatgtgtt
     1441 ctgctttaca aaacttacga atatttcagc atccgatatt cctgacaggt cagctacata
     1501 ggggataaag gcatcaacca tgcccatggc agccagctga tcgttcagga ttttttcgta
     1561 tgaaaatttg aattttggaa aatatactat gcactttccg aactcttcaa tctcatccaa
     1621 ttcggaggta aggctcttcc agaggtcaga atcaaatgaa ctgttaaaag atttaagtcc
     1681 gccttcaggg acaattacta tcatggtgaa atttgttctt ccgtagggca tttctattgc
     1741 cctgaatttt tccctgtaca cgacttttgc ccctatttca ccgttcatgg ttggtacatt
     1801 tacagccggg agattgtcgg gataaaattg ccgctcagtt gttttcgatt tatcgaactt
     1861 gtaactccag tcgcctttaa aatatagagc attcattatg aacatcacag tttcatcaga
     1921 aatttgatca agaactttcg ggattttacc gctggtgtta tcgcttgccc acttgttgat
     1981 tgtattgaga gcagatggcg cattaaaatc aagtccttcg atctttgcct taaagttctc
     2041 tttcatcgaa ttgatgaact gagcttttac gttgaatcca agcctgtaaa atatggcatt
     2101 tgcaagtgca aaagtcacct ttgggtcggc tttcagaagt tgctcaacca gacttttata
     2161 tgcctcatta atatcctctg tttgcattcc ggaaggatat ttaagcgtac tcctcaactg
     2221 ggtaaaagta tttccagagc atccattcag gagcattgta agagccgttg aagcgctcag
     2281 tggcgaaagc attaagtttc tgtcttcggt ttcagcaact tttgtgaaca aatcaacacc
     2341 aaattcattg ccggaagaga taacctgcga agctgcaggt gtcagttcaa tttttgctgg
     2401 cgtaagatcc ggctcaccgg gctgtttctc acaggaaacc aacagaaaaa atatggctaa
     2461 tagacctgct gctgttaatg cctttgttct cataatatct gatttttatg ttttgttaag
     2521 ctttccatta tctatacgaa acaaaccacc ggattgctgc agaaaacgaa aaaaattttt
     2581 ttaaaatggc tgtcacccaa ttctgtgaca gccatttatt cggaaaaacg agcttggaat
     2641 attagactcc gaattataag atctcctgaa gttcgtcagc ggctcaaaga ttactgtgaa
     2701 gaagttcgac agctttgtta aggtcttctt tagccttctg gcattcaccc agacctttaa
     2761 agccgaggcc ttaagttgtc tctccccctc ctcaaccatt tccccaaact gggggaagga
     2821 gcttatagtg tgcattccat ctctctcctc cccagatgcg gaggccggga ggggcattcc
     2881 gggtattcta aaaatcccat gggcttcgtg gtgtgcccgc cggtgacgct ttcgggcgct
     2941 ggctgaatgg cagtattttt cctgcgtgta catcataaag ccttttgtgt gtctcctttg
     3001 cagcctccac gagctcttta taaggccggt cggttacatc cacaaagcct atgttgtagt
     3061 tttcaccgtc gaatcttccc agcacaggct gatcgcgcca ctggaaccag taagcaccaa
     3121 caaatgcagc tgttgcagca gcctgttcaa cataatacct gtaggcaatg cctctctctt
     3181 tctggttcat tgcctgtaca agccctgctc ccagaccatt ttccggtact cctatatgga
     3241 actcgccgat gagaaccggc cttcctgtat aacggtaaac cctctcaatc tgggcaacag
     3301 gctcatattc atacacattt atactgcaaa catcaaaaat acgacccgtc ttcagcagtc
     3361 cggcagaagg agatcctcca aatcttattc cgaggttcag atggttggga tcatattttt
     3421 ttattgccga acagataagc tgtagatatt tttcgaatgc tccaagtaca aactccctgc
     3481 gtcttcctgg tgtatctccg gcagacagaa actcttttaa cctcttcttt atttcgttat
     3541 cgggtcctgc aagtatcatg tcaaccactt cgctttcccg gccatcccat gcaggttcat
     3601 tgccaacaaa atatccaatc agaagcgggt catttttcaa gggttcaca
//
LOCUS       DAOSNB010000269         3639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_25552_length_3639_cov_4.300781, whole genome shotgun sequence.
ACCESSION   DAOSNB010000269 DAOSNB010000000
VERSION     DAOSNB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25552_length_3639_cov_4.300781"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLG42_09675"
     CDS             complement(<1..368)
                     /locus_tag="PLG42_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOU31517.1"
                     /translation="MDCNKKDTVIELKSLTTGYRKAGTLYPLVGPVNISAQSGEFIAI
                     IGRNGIGKSTLLKTIAGLLKPLEGDVIIKRKKITEYRRNELAVTTAYVSTEQIRADSM
                     KVGELVELGRYPYASWTGRLS"
     gene            complement(372..1421)
                     /locus_tag="PLG42_09680"
     CDS             complement(372..1421)
                     /locus_tag="PLG42_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOU31518.1"
                     /translation="MVNTNAKYRLSGRNRTKILLLTIFLLTALLFSLDIILGSVTIKL
                     KDIFDVLLNKSDSEIYSVIVKFRIPKAITALLVGVSLSVSGLQMQTIFRNPMAGPYVL
                     GISSGASLGVAVLILGFSDYFTAKSLELLGNWSLAAASWAGAGIVMVIIMLISIKIKD
                     ILAILILGIMLSGGISSIVSIMQYFSSETMLKSYVIWTMGSLGNVSSEQLEVMALCIA
                     AGLIISIFSLKMLNAILLGETYAETLGVNIKKARILVFVSTSMLAGTVTAFCGPVGFV
                     GIAVPHIVRMIFKTSDHRVLMPSVILAGASLMLVSDIISQLPGSERVLPVNAVTSLIG
                     IPVVIWVILKTRKYA"
     gene            complement(1440..2618)
                     /locus_tag="PLG42_09685"
     CDS             complement(1440..2618)
                     /locus_tag="PLG42_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOU31519.1"
                     /translation="MPENKSSTYHKLIEGIFYVVIIIFVFNSCKNKNALPDKTSEYQP
                     VSVKYARHFDLIPENGYTRLSIINPWQKSSGLKFEYYLLQKGIKPPAGFDTSKIIKVP
                     VEKVVLMSTTYIPMVMAIGAGEAVKGISGVNFVFDTIVRGKIIEGKIVDIGYEENLNK
                     ELIFKLSPDLIIAYGVGSEAAGNMRKLEESGIKILFNADYLEENPLGRAEWIKVFGAL
                     FCREKEADSVFRAVEKEYNEIKSFISERVKNRPNVMLGLPWKDSWFISPGNSYISVII
                     NDAGGNYLWKDSKADYSIPLGIENVFVKASKADFWINTGDATSIKAIVSIDKRLGFIN
                     PVRKGNVYNNNKRVSEGGGNDYWESGTLYPQRILKDIASILHPELFPGYEWYYYRKIE
                     "
     gene            complement(2629..>3639)
                     /locus_tag="PLG42_09690"
     CDS             complement(2629..>3639)
                     /locus_tag="PLG42_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31520.1"
                     /translation="SLIGLTSCSLQDDPPEPFYDNFLSSKGLFVLNEGNFMAGNGSVS
                     FYSTDSMKIYNDIFSNANSRPIGDVPNHMGIIGDRAFIVVNNSGKIEVTSCKTMKSIA
                     VIPGLTSPRQILFINNNKAYVSSLYSTKIIVINLENYTINSYIDIRRTSESMILCEGK
                     AYISCWSGGDELVVVDTSSDTVIDSVKVGHEPESMVIDRNGKLWVLCSGSYTGEFYPE
                     LVVINTVTDSVEKKFQFGSKLMYPCCLKINGTGDTIYYIERSIWRMAVTSDQLPAEPF
                     IRASGRNFYKIGVEPESGRLYATNAIDYQQKGFLLIFNRDGTVADSVKVGIIPGYICF
                     KQ"
BASE COUNT         1113 a          762 c          609 g         1155 t
ORIGIN      
        1 gacaacctgc ctgtccatga agcataggga tatctcccca gttctacaag ctctccaact
       61 ttcatactat cggctctaat ctgttcagtt gaaacatacg ctgttgttac tgcaagttcg
      121 ttgcgcctgt attcggtgat ttttttcctt tttataatta catctccttc aagaggttta
      181 agtaaaccgg ctattgtttt cagtaatgtg ctcttgccaa ttccgtttcg gccgattatc
      241 gcaataaatt ctccactttg agcagatatg tttactggcc caacaagcgg gtacagagta
      301 cctgcttttc tgtaacctgt tgttaaagat ttcagttcga taactgtgtc ttttttgttg
      361 caatccatct atcaggcata ttttctggtt ttcagaataa cccatataac aacaggtatt
      421 cctataagtg aagtgacagc atttacagga agcactcttt cggaaccagg aagctgggaa
      481 attatgtcgc ttacaagcat taaagaggcg cctgcaagta ttaccgaagg cattagaacc
      541 cggtggtcgg atgttttgaa aatcatcctt acaatatgag gtacagctat acctacaaat
      601 ccaacaggac cacaaaaggc ggtaacagtt ccggctaaca tactggtgga aacaaaaacc
      661 aatattcttg cctttttgat atttacaccg agtgtttctg cataggtctc acctaataat
      721 atggcattca gcatttttaa tgaaaatata ctgataataa gtccagctgc aatacataat
      781 gccatcacct ctaattgctc cgacgaaaca tttccaaggc ttcccatagt ccagattaca
      841 tacgatttca acattgtttc actgctgaaa tactgcataa tactgacaat ggatgagatg
      901 cctcccgaca acattatgcc aagtattaat atggcgagaa tgtctttaat ttttatggaa
      961 ataagcataa taattaccat aactattcca gctcctgccc atgaagctgc agcaagcgac
     1021 cagtttccaa gcaactcaag tgattttgcg gtaaaataat ccgaaaaacc aagtatcaga
     1081 actgcaactc ccagactcgc accggaacta ataccaagta cataagggcc tgccattgga
     1141 ttacgaaata ttgtttgcat ctgtaaacca ctaaccgaca aagagacgcc tacaagaaga
     1201 gccgtaatag ccttgggaat tctgaatttt acaataactg aatatatttc agaatcagat
     1261 ttattcagca gaacatcaaa aatatctttc agttttattg tcactgagcc aagaattata
     1321 tcaagtgaaa ataagagtgc tgtcaaaaga aatatcgtta aaagaagaat ttttgttcta
     1381 tttctgccag ataatctata tttcgcgttt gtattgacca taagtatttt ttctgaagct
     1441 cactcaatct ttctgtaata ataccattca taaccgggaa acaattccgg atggagtatc
     1501 gatgcaatat ctttaagtat gcgctgggga taaagtgtgc cgctttccca gtaatcattg
     1561 ccgccacctt ctgaaactct cttattattg ttgtatacat tgccttttct gaccggatta
     1621 ataaacccaa gtcttttgtc aatagaaaca atggctttaa tgctggttgc atctccggta
     1681 ttaatccaga aatcagcttt cgacgctttt acaaatacat tttcaatgcc aagaggaata
     1741 gaataatcag cctttgaatc tttccaaagg taattgcctc cggcatcatt gatgataacg
     1801 gatatatatg aattacctgg tgatatgaac cagctgtcct tccagggaag gccgagcatg
     1861 acattaggcc tgtttttaac cctttcactt atgaatgatt taatttcgtt gtattctttt
     1921 tcaacagccc ggaaaacgga atcggcttct ttttccctgc aaaaaagggc tccgaaaact
     1981 tttatccatt cggctctgcc aagtggattt tcttcaagat aatcagcatt aaaaagtatt
     2041 tttattcctg actcttccag ttttctcatg tttcctgcag cctcgcttcc caccccatag
     2101 gcaataatca ggtcgggtga cagtttgaaa attagctctt tattaagatt ttcttcataa
     2161 ccaatatcaa caatcttacc ttctataatt ttccctctta ctattgtgtc gaatacaaaa
     2221 tttactccgg aaatgccttt aacggcctcc cctgccccta tcgccattac catgggaata
     2281 taggttgttg acataagaac aaccttttct accggaactt ttataatttt tgaagtgtca
     2341 aaacctgcag gaggttttat tcctttttgc aacaaataat attcaaattt taatcctgat
     2401 gatttctgcc aggggttaat tattgatagt ctggtatatc cgttttcagg tataagatca
     2461 aaatgccggg catattttac agaaacaggc tgatattcgg atgttttatc gggtaatgca
     2521 tttttgtttt tgcacgaatt aaaaacaaaa ataattataa cgacataaaa tattccctca
     2581 atcagtttat ggtatgttga tgatttattc tcaggcatta tttcgtcctt attgtttaaa
     2641 acaaatataa cccgggatta taccaacctt aactgaatct gctactgttc cgtctctgtt
     2701 aaaaatcaac agaaaaccct tctgctggta atcaatagca tttgttgcat agagcctgcc
     2761 actttccggt tcaactccta ttttataaaa attacgaccc gaagccctga taaacggctc
     2821 tgcaggtaat tggtcggatg tgacagccat cctccagata gatctttcga tataatagat
     2881 agtatctcct gtaccattta tttttagaca gcagggatac ataagttttg aaccgaattg
     2941 aaatttcttt tcaacactat ctgtaacagt attaattaca acaagttccg gataaaattc
     3001 gccggtataa cttcccgaac aaaggaccca taatttccca ttccggtcaa taaccatgct
     3061 ttcaggctca tgaccaactt ttactgaatc gatcacagta tcactggatg tatcaacaac
     3121 caccagctca tcccctcccg accagcagga aatgtaagcc tttccttcac aaaggatcat
     3181 tgattctgat gttcttctta tatcaatata actattaatt gtataatttt ccagatttat
     3241 aactattatt ttagttgaat aaagactgga aacataggcc ttgttattat taataaaaag
     3301 aatctgtctt ggtgaggtta aacccggaat aactgcaatt gatttcatgg ttttacagga
     3361 ggtcacttca atttttccag aattatttac aacaatgaat gctctgtcac ctataatgcc
     3421 catatgattg ggcacatcac caatgggtct cgaattagca ttactgaaaa tatcattgta
     3481 aatcttcatg gagtctgttg aatagaatga cacagagcca ttacccgcca tgaaatttcc
     3541 ttcgttaaga acaaataacc cttttgaact gagaaaatta tcgtaaaatg gttcaggtgg
     3601 atcatcctgc agggaacacg aagtgagccc gattaagct
//
LOCUS       DAOSNB010000270         3613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_25819_length_3613_cov_4.269533, whole genome shotgun sequence.
ACCESSION   DAOSNB010000270 DAOSNB010000000
VERSION     DAOSNB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25819_length_3613_cov_4.269533"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLG42_09695"
     CDS             <1..730
                     /locus_tag="PLG42_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31521.1"
                     /translation="GYSQEQPWHNSAYNADGSLRTEYMKRLEKILNRADELGMVVILG
                     LFYFGQDERLKDEAAIRNAIDNVLNWLFVRGYKNILIEVNNECNVRYDHDVLKPERVH
                     ELIELVKQKNRGGFRFYAGTSYGGGFIPLPNVVQASDFILIHGNGVSNPDNILAMVNK
                     TRAVAGYTPKPVLFNEDDHYDFDKEWNNFVAAIKGYASWGYFDYRMKDEPFECGYQSV
                     PVDWGINSDRKKAFFNLLKEITGF"
     gene            807..2687
                     /locus_tag="PLG42_09700"
     CDS             807..2687
                     /locus_tag="PLG42_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOU31522.1"
                     /translation="MKKYLSNLMIVLTLILFFTACSKDASAHYSGQVPDHPRILLLKG
                     EENQVKANINTDYYWKIVHDMIIAECNVIISNPPVERILIGRRLLDKSREALKRIFYL
                     SYAYRITGNRAFLDRAEKEMLAVSAFTDWNPSHFLDVAEMTMAVAIGYDWLYESLSEN
                     TRQVVRDAIINKGLKPSLDSKYNSWLRATHNWNQVCNAGMTYGALAIWEYDSTLASDI
                     INRACESILLPMEDYKPDGAYPEGYGYWGYGTSFNVMFISAYEKAFGKKFESPATESF
                     LKTAYYMVNMSGPTGQCFNYSDNGSGTGLHPAMFWLASRLGDYSVLYEEMKFLGQMKR
                     ASDRLLPAMMIWNAGITKTLVKEPARLMWTGGGKNPVALMRTSWSNPDAIFVGLKGGS
                     PRVNHGHMDVGSFVMESEGVRWAMDFGAQDYNSLEQKGVDLWNMSQNSQRWEVFRYNN
                     FVHNTLTINQQLQRVDGKAEIISSSDNPDFMNAVMDLAPVYSGQLTSAKRGVAIADRK
                     YVIVRDEIQPVSDNTTVRWTMLTSASVKITDTGTAELTGNGKRLLLKVTQPSDATIKT
                     WPTDPPHDYDAPNPGTTLAGFEIVVPAGRTTAIEVLLIPGNTDPSSAMGKGELKNWPA
                     VK"
     gene            3109..>3613
                     /locus_tag="PLG42_09705"
     CDS             3109..>3613
                     /locus_tag="PLG42_09705"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOU31523.1"
                     /translation="MKRSIRSDNIYYINIEKNLKNIKPLPLSILGYNIEYILPETNNN
                     SLLSRISNVFVTDSSLFISDGNRILHFNINGRFLKQIGTQGRGPGEYVSVSDFYSDII
                     DNEIYILSPFRMLVYDFEGNFIRSFKIDFPCNQFILSNDTLVFYPFNIPDPESESCEL
                     YFTNKSGL"
BASE COUNT         1147 a          663 c          764 g         1039 t
ORIGIN      
        1 aggatactca caggaacaac catggcataa ctcggcatat aatgccgacg gcagcctgag
       61 aacggaatat atgaaacgcc ttgagaaaat tcttaaccgt gccgacgagc ttggaatggt
      121 tgtaattctg ggactctttt atttcggcca ggatgaacgg ctgaaagatg aggcggcaat
      181 aagaaacgca attgacaatg tgctgaactg gctgtttgtt agaggttaca aaaatatcct
      241 cattgaagta aacaacgaat gtaatgtaag atatgatcac gatgttctca agccggaaag
      301 agttcacgaa cttattgaac ttgtaaaaca gaaaaacaga ggaggattcc gcttttatgc
      361 cggtacaagc tacggaggag gattcatacc tttgcccaat gtggtgcagg catcagattt
      421 tatccttatt catggaaacg gagtctcaaa tcccgacaac atccttgcaa tggtcaacaa
      481 aaccagagca gtggcaggct acaccccaaa acctgtactg ttcaatgaag atgaccatta
      541 cgactttgat aaggagtgga ataactttgt ggcagcaatt aaaggatatg cttcatgggg
      601 ttattttgac tacagaatga aggacgagcc ttttgaatgc ggataccaga gtgtgccggt
      661 tgactggggt ataaactctg acaggaaaaa agcattcttt aatctgctga aggagataac
      721 aggattctaa gggaagttgg aaaagatgga tttcaattaa taaatagata atgttaaaaa
      781 aagtggttat taattatcga cgaattatga agaaatattt atcaaatctg atgatagttc
      841 ttactctgat tttgtttttt actgcatgtt caaaagatgc ttctgcacat tactccggtc
      901 aggtacctga ccatccgaga attcttcttc tgaaaggtga agaaaatcag gttaaggcga
      961 atattaatac agactattac tggaagatag ttcacgatat gattattgct gaatgcaacg
     1021 ttatcatcag caatcctccc gttgagcgga tacttatcgg cagaaggctt ctcgataaat
     1081 cacgtgaagc actcaaacgg attttttatc tgtcatatgc ataccggatt accggcaaca
     1141 gggcatttct tgaccgtgca gaaaaagaga tgcttgccgt atcagctttt actgactgga
     1201 acccttcgca ttttctcgat gtggcagaaa tgaccatggc agttgccata ggctacgact
     1261 ggctgtatga gtcgttatca gaaaatacca ggcaggttgt gcgggatgcc ataataaata
     1321 aaggacttaa accttctctc gattcaaaat ataacagctg gctcagagcc acacataact
     1381 ggaatcaggt atgtaatgcc ggcatgacgt atggtgcact tgcaatatgg gaatacgatt
     1441 cgactcttgc ttcggacata ataaacagag catgtgaatc tatcctcctt cccatggagg
     1501 attataagcc cgatggtgca tatcctgaag gctatggcta ctggggatat ggcacaagct
     1561 ttaatgtgat gtttattagc gcatatgaaa aagccttcgg aaagaaattt gaatcaccag
     1621 ccaccgaaag ttttctgaag actgcctatt atatggtaaa tatgtcgggc cctaccggtc
     1681 aatgtttcaa ctattccgat aacggttcag gcaccgggct gcatcctgcc atgttctggc
     1741 tggcttcacg gcttggcgat tattcggtac tttacgagga aatgaaattc ctcgggcaga
     1801 tgaaaagggc aagcgacaga cttcttcctg cgatgatgat ctggaatgca ggtataacta
     1861 aaacattagt taaagaacct gccaggctta tgtggacagg cggcgggaaa aaccctgtcg
     1921 ccctcatgcg tacatcatgg agcaaccctg atgccatatt tgtcgggttg aaaggaggct
     1981 ccccgcgagt aaaccacggc catatggatg tgggttcttt tgttatggaa tcggaaggag
     2041 tgcgctgggc aatggacttc ggtgcccagg attataattc cctcgaacag aaaggggtcg
     2101 acctgtggaa tatgtcgcag aactcacagc gctgggaagt gttcagatat aacaactttg
     2161 tacataacac cctgacaatt aatcagcaac tccagcgggt tgacggtaaa gcagaaatta
     2221 tatcatcatc agacaaccct gatttcatga atgctgtcat ggatcttgcc cctgtctaca
     2281 gcggacaact gacatcagcg aagagaggag tggcaatcgc agacagaaag tatgtaattg
     2341 taagggatga aattcagcct gtaagtgaca atacaacagt aagatggaca atgcttactt
     2401 cagcatcggt aaaaattaca gacaccggca ctgctgaact tacaggtaac gggaaaaggc
     2461 ttttattaaa agtcacacag ccttctgacg caactattaa aacctggcca accgacccac
     2521 ctcacgacta tgatgcaccc aatccgggta caacactggc aggttttgaa atagttgtcc
     2581 ctgcaggcag aacaacagcc attgaggtgc ttcttatccc tggaaatact gacccttcat
     2641 ctgcaatggg gaaaggcgaa ctaaaaaact ggccggcagt aaaataaggc tgatataccg
     2701 cccttaggat tatttcattt gtatatcaac tatttatatt ttttcaattg tcagactcga
     2761 gaaaatacca ttttgtctaa gaaggctttt atgaaagccc attctttaag cagggtaata
     2821 gtagggtaat attttatggg tgtttatttg aaaaaaattt ttttaaaaat tttttatttt
     2881 tcaggtaaca attttaccct gatctctatt aaacttaata aaaaacaatg aacctgaatt
     2941 gacaggttct gttttattta tttttattaa tttaaaggtg tgaaaatata atattttatg
     3001 taattgatta attataaatt ttttacagtc ttgaaaatta taatgttgtt gataattttc
     3061 ccttttctaa tgtcaggatt attatcatgc acctgtacag atcgttctat gaaaagaagc
     3121 attagatctg ataatatata ttatatcaat atagagaaaa acctgaaaaa tataaaaccc
     3181 ctaccattaa gcatattagg ctataatatt gaatatattc ttccggaaac aaataataat
     3241 tctttattaa gtagaatatc aaatgtcttt gtaactgatt cttctttgtt tataagtgat
     3301 gggaatagaa tacttcattt taacataaac ggtagattcc tgaaacagat aggcacacag
     3361 ggaagggggc cgggagaata tgtatctgtt tcagattttt attcagatat tattgataat
     3421 gagatttata tattgtcacc gttcaggatg ctagtttatg attttgaagg aaattttata
     3481 agaagtttca aaatagattt tccatgcaat caatttattt tgagtaatga tactctggtt
     3541 ttttatccat tcaatatacc cgacccggaa tcagaatcct gtgagttata tttcacaaat
     3601 aaatcagggc ttt
//
LOCUS       DAOSNB010000271         3568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26258_length_3568_cov_3.911472, whole genome shotgun sequence.
ACCESSION   DAOSNB010000271 DAOSNB010000000
VERSION     DAOSNB010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26258_length_3568_cov_3.911472"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..743)
                     /locus_tag="PLG42_09710"
     CDS             complement(108..743)
                     /locus_tag="PLG42_09710"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOU31524.1"
                     /translation="MKQLLIIILTSSIFSALSGQSDPEAVKILDRFSSKALTAPSVSM
                     KFSVISINATENTRDTLDGSLIMAKDQYRLELPNNIIWFNGSATWNYLVAEKEVTITR
                     PDRKDDTFMSRPSSIFTIYKKGYKIRLLEDNLNTYVIDLYPEDLNSDMVRIRLTIGKS
                     LLDLINAEYKRKDGVTIIIAVKEYNLKIVPDPSAFTFNPKNYKGVEIIDMR"
     gene            complement(753..3212)
                     /locus_tag="PLG42_09715"
     CDS             complement(753..3212)
                     /locus_tag="PLG42_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOU31525.1"
                     /translation="MKSSKGEKSNNGESGKKNSTGSSRGKKSGSGEKFRFLFGIVLTG
                     AAAYLLIAFIAFLFWWKTDLSLATDQVISGPELQVKNWSGKLGVWLSEFFITNGFGLG
                     AFFIPLIIAAAGLKLLNFPSLRFWKLLLKFTFATILLSLLAGYVFGMAGGFLGSGPGG
                     AQGYFVVKWLNSFIGKPGTGILLIVLTLLFLVFIMNIHPETISKIIQALFTKYLKKQQ
                     PAGEIKSDTDNEKDLKNEAETEEEEEEEEEAGDIIVKPVYPLNDENQFEEIVPDEHDG
                     GIIRVLGDSELLTVEPVSKDVPLKVTRAKEGEQLSEEEVEKIMENYDPRLDLPRYKFP
                     PVSLLKEYKSQSSIDNREIIENKDNIVKTLADHKIPIKNITATVGPTVTLYEVVPERG
                     VRLARIKSLENDIALNLSALGIRIIAPIPGRGTVGIEVPNKNPEIVTMRSLITSRAFQ
                     ESKFELPLALGRTITNEPYIADLTKMPHLLVAGATGQGKSVGINAIITSLLYKKHPAE
                     VKFVLIDPKRVELPLYAKIERHFLAKLPGEEDAIITDTQKVIYTLNSLCREMDNRLEL
                     LKLAQARYIKEYNEKFISRKLNPNNGHRYMPYIVLIIDEFADLIMTAGREIEGPIGRL
                     AQLARAIGIHLIISTQRPSTNIITGFIKANFPTRIAFRVFSSVDSRTILDATGAEHLV
                     GRGDMLISAGAELERLQCAFIDTPEIEALTEFIGSQQGYSDAMHLPEYTPETEGGEKD
                     IDLNKRDPMFEDAARLVVQTQQGSTSLIQRKLSIGYARAGRIIDQLEAAGIVGPFEGS
                     KAREVLVSDMIALEQILKRLE"
     gene            complement(3302..>3568)
                     /locus_tag="PLG42_09720"
     CDS             complement(3302..>3568)
                     /locus_tag="PLG42_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31526.1"
                     /translation="FPSVPDKVYDIWYTDEIKVKNSNASTPFKQIDGVLMSFYYILGG
                     SEMKFEAEAVYKKTVSDKIFERRPKYKLISKKDMDKIISDIVNL"
BASE COUNT          923 a          760 c          703 g         1182 t
ORIGIN      
        1 ctcaccgaag agaacctgac tgagcatctc cgatttgtgt gtgggcattg ccctgagagg
       61 tacaaatacg ttttcacaaa caaattgttt catattgaaa ttaaatatta gcgcatgtca
      121 attatttcca cgcctttgta attcttcgga ttgaaagtga aagccgatgg gtcggggaca
      181 atcttaagat tatattcctt caccgctatt attattgtta ccccgtcctt tcttttatat
      241 tcagcattaa taagatccag taatgatttt ccgattgtca gccttattct gaccatgtcg
      301 ctgttcaaat cttcaggata aaggtctata acataagtgt ttagattgtc ttcgagaagc
      361 cggatcttat atcctttttt atagattgta aaaatagatg aaggacggct cataaaagtg
      421 tcatctttcc ggtcgggacg ggtaattgta acttcttttt ctgcaacaag atagttccat
      481 gtggctgagc cattaaacca tatgatatta ttgggcagtt cgagccggta ttgatctttt
      541 gccattataa gacttccatc aagtgtgtcc ctagtatttt ctgtagcatt tatgcttatt
      601 acactgaact tcattgacac agatggggct gtcagtgctt ttgatgagaa tctgtcaaga
      661 attttaacag cttccgggtc agactgtcct gaaagtgctg agaatataga tgaggtcagg
      721 attattatca gtaattgttt cattacagtt ttttattcaa gtctcttcaa aatctgttcc
      781 agagctatca tgtcgcttac aagaacttcc cttgccttgc ttccttcgaa tggtcctact
      841 attccggcgg cttcgagctg gtcaattatt cttcccgccc gtgcataacc aatggaaagc
      901 tttctctgaa taagggatgt ggagccctgt tgtgtttgca ctaccagacg tgctgcatct
      961 tcaaacatgg gatctctttt gttaaggtcg atatcttttt ctccaccttc tgtttcgggg
     1021 gtatattccg gaaggtgcat ggcgtcgcta tagccctgtt gcgaaccaat aaattctgta
     1081 agtgcctcaa tttcaggtgt gtctatgaat gcacactgca gcctttcaag ttctgcacct
     1141 gccgatatga gcatatcgcc acgtcctaca agatgctcag cccctgtggc atcgagaatg
     1201 gtgcgggaat ccacagatga aaatactctg aatgcaatcc ttgtcgggaa gttagctttg
     1261 atgaaccctg taataatatt tgttgatgga cgctgagtgc tgataattaa atgaattcct
     1321 attgcccttg caagctgggc aagtctgcca atcggaccct ctatttccct tccggcagtc
     1381 ataataagat ctgcaaactc atcaataata agcactatat agggcatata gcggtgccca
     1441 ttgttagggt tcaactttct cgatataaat ttctcgttgt attccttaat atatcttgcc
     1501 tgtgccagtt tgagcaattc aagtcggtta tccatttccc tgcacaggga attaagggta
     1561 tatataactt tctgtgtatc tgttattatt gcatcctctt caccgggcag ttttgcaagg
     1621 aaatgccgtt cgatcttagc atatagcggc agttccactc tcttaggatc aataagtaca
     1681 aactttactt ccgcaggatg ttttttataa agcagtgatg ttatgatggc atttatccct
     1741 accgatttgc cctgtccggt tgctcctgca acaagaagat gtggcatttt tgtcagatct
     1801 gctatgtagg gctcgtttgt tattgtacgt ccaagggcaa gcggcagttc gaattttgac
     1861 tcctgaaacg cccttgaagt gatgagggat ctcattgtaa caatttcagg gtttttgttt
     1921 ggcacttcga tgcccaccgt gcctctaccc ggaataggtg ctataattct gatccccaat
     1981 gctgaaagat taagggctat gtcgttttca agcgatttta ttcttgcaag cctcacccct
     2041 cgttccggaa cgacctcgta gagagttacc gtaggtccaa cagttgcagt gatatttttt
     2101 atgggaatct tgtgatcggc aagggtttta acaatattat ccttgttttc gattatttcg
     2161 cggttatcaa ttgagctctg gctcttgtat tccttcagaa gtgaaaccgg aggaaatttg
     2221 taccttggca ggtcaagcct cgggtcgtaa ttttccatga ttttttccac ttcctcctca
     2281 ctgagctgtt ctccttcttt tgccctggtt actttcaggg gtacatcttt gctgacaggc
     2341 tcaacggtta atagctctga atctccgagt acacgtataa ttccaccgtc gtgttcatcg
     2401 ggcactattt cttcaaactg attttcatca tttagcggat agaccggctt tacaataata
     2461 tctcctgctt cttcttcttc ttcttcttct tcttctgttt ctgcttcgtt ttttaaatcc
     2521 ttttcgttat cggtatcaga cttaatttca ccagcaggtt gctgtttctt taaatatttt
     2581 gtaaatagag cctgtataat tttacttata gtttcaggat gaatattcat aataaatacc
     2641 aggaacagga gagttagcac aatcaataat attccggtac caggctttcc gatgaatgaa
     2701 ttgagccatt taacaacaaa ataaccctgt gcccctccag ggccgctgcc aagaaaacct
     2761 cctgccatgc cgaaaacata tccggcaagt aaggataaaa gaattgttgc aaatgtgaat
     2821 ttcagaagca gtttccagaa tctcagactc gggaaattca gtagcttaag cccggcggct
     2881 gcaataatca gcggtatgaa aaatgccccc agtccgaaac cgttggtaat aaaaaactcc
     2941 gaaagccata cacccagttt acctgaccag ttcttaacct gtaattctgg accggatatt
     3001 acctgatcag ttgcaagact gaggtctgtt ttccaccaga acaggaatgc aataaaagca
     3061 atgagaaggt aagcagctgc accggttaat acgattccaa aaaggaaacg gaatttttca
     3121 ccacttcctg atttttttcc tctgctgctt ccggtactgt tttttttgcc ggattctccg
     3181 ttattgctct tttctccttt agatgatttc attacctcca ttgatgataa taactctgac
     3241 ctgtcggttt ccagagtcca aaggtaaata ttttaaaatg atttttccgg aaatgaaaaa
     3301 gctaaagatt aacaatatcg gaaataattt tatccatgtc ttttttcgaa atcagcttgt
     3361 attttggcct tctttcgaaa attttatcag aaacggtttt cttatatact gcttcagctt
     3421 cgaatttcat ctcggaaccg cccagaatat agtagaaact cattaaaact ccgtctatct
     3481 gtttgaaggg agtggaagca tttgaatttt ttacttttat ctcgtcggta taccagatgt
     3541 cataaacttt gtcgggtact gaaggaaa
//
LOCUS       DAOSNB010000272         3555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26374_length_3555_cov_3.899143, whole genome shotgun sequence.
ACCESSION   DAOSNB010000272 DAOSNB010000000
VERSION     DAOSNB010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26374_length_3555_cov_3.899143"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="PLG42_09725"
     CDS             complement(<1..173)
                     /locus_tag="PLG42_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU31527.1"
                     /translation="MKYFLVAGEASGDLHGANLIRGILKADPGAEFRCWGGDKMHEAG
                     AQLLMHYRAHAIMG"
     gene            complement(170..472)
                     /locus_tag="PLG42_09730"
     CDS             complement(170..472)
                     /locus_tag="PLG42_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31528.1"
                     /translation="MNKYLKYDKFLTGFIPGIILPFLIALLIFLFTSKNQTLLDYYFE
                     ILRRGFMTHGITICVIPDVILFFVFNRADMLKSARGMLGATIFWVLVMLVNKIVPL"
     gene            complement(469..1257)
                     /gene="surE"
                     /locus_tag="PLG42_09735"
     CDS             complement(469..1257)
                     /gene="surE"
                     /locus_tag="PLG42_09735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOU31529.1"
                     /translation="MVVEQGDRLILITNDDGLYAAGLRTLLEIAEEFGKVVLISTMES
                     MSGMSQALTVKNPIRVKLLEENEKHRIYTCNGTPTDSVKLAINQLTERMPDWLLSGIN
                     HGSNASVSVLYSGTMAAALEGCLYNIQSVGFSLNDFSSGADFTICKKYIRIILNSLTH
                     NRLPEGVCLNVNIPSVPENEIRGIRVCRQAKGSWKEEFEKRKDPMGKTYYWLTGYFVN
                     REPDATDTDEWALANNYVSVVPIQVDMTAHAFINELEKWFNNSK"
     gene            1386..2966
                     /gene="pheT"
                     /locus_tag="PLG42_09740"
     CDS             1386..2966
                     /gene="pheT"
                     /locus_tag="PLG42_09740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU31530.1"
                     /translation="MKISYNWLKEYLQIDLDPHDVAEILTNTGHEIEETEEWYSVEGG
                     LKGIVIGEVLSSQKHPDADRLTVNIVDVGGERPLTIVCGAPNVAAGQKVPVALPGSFI
                     FKGDEKIEIKTSRIRGQISEGMICAEDEIGMGTSHEGIMVLSADAKPGTPAAEYFGVE
                     KDFVFTIGFTPNRIDCASHIGLARDLAAYLAVNRGMDIRLKMPSIESFKPENSRETFE
                     VIIENTRDCPRYTGVNIFDVKVAESPQWLKNRLRAVGLSPINNVVDITNYVLYECGQP
                     LHAFDADMITGQKVIIKNLPDGTKMTTLDNVERVLSAKDLIICNESEGMCIAGVFGGL
                     KSGVTEATRNVFLESAYFNPVSIRKTAKRHSLNTDASFRFERGADPEITVWALKRAAT
                     LIRDIAGGKLLADIRDVYPVKIPNPVITISCDRINRLIGKDLPYDKIKKIISLMDIKI
                     LSENLPEMKIEIPARKVDVQKEADVTEEILRIYGYDNVEVGNHVNSTLSYTEKPDRHK
                     LMNLVSEMLSANGFSEIM"
     gene            3033..>3555
                     /locus_tag="PLG42_09745"
     CDS             3033..>3555
                     /locus_tag="PLG42_09745"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOU31531.1"
                     /translation="MANPLSSDLNAMRQSLLPGGLSTIARNINRQNYDLKLFEFGNCY
                     FFNNTTGVKRVDRYSERMSLDLFMTGNRAKHTWNSPKTPADFFYMKSFTEMVLQRTGL
                     DADSLKQAESEKSYFTESLVYLVNGKPVAETGKISPSYLKKFEIEQDVYYSHIDWDYL
                     LEVIKNNAITYREL"
BASE COUNT         1088 a          704 c          774 g          989 t
ORIGIN      
        1 cccattatgg catgagcgcg atagtgcata agaagttggg caccggcttc atgcatttta
       61 tcacctcccc agcagcggaa ttctgctccg gggtcagcct tcaggatacc cctgataagg
      121 tttgctccat gaagatctcc cgaagcctct cctgctacaa gaaaatattt cataacggga
      181 caatcttgtt tacaagcata accagtaccc agaaaattgt tgcaccgagc attccgcggg
      241 cagacttcag catgtcggcc cggttaaata caaaaaacaa aatcacatcg ggaataacgc
      301 aaatcgttat gccgtgagtc ataaaacctc ttctcagtat ttcaaaataa taatccagaa
      361 gtgtctggtt tttggaagta aaaagaaaaa tgagcagtgc tattaaaaaa ggcaggatta
      421 ttccgggtat gaatcctgtt aaaaatttat cgtatttaag gtatttattc attttgagtt
      481 gttaaaccat ttctcgagtt cgttgatgaa ggcatgagct gtcatatcaa cctgaatggg
      541 gactactgac acatagttat ttgcaagtgc ccattcatcg gtatcagtgg cgtcgggttc
      601 acggttaaca aaatatccgg taagccagta atatgttttg cccatgggat ctttcctttt
      661 ttcgaattct tctttccagc taccttttgc ctggcgacat acacgtatac ctcttatttc
      721 attttcgggt acagaaggta tattaacgtt aaggcagacg ccttcgggga gcctgttgtg
      781 agttaaagaa ttaagtatta tcctgatata tttcttacat atagtaaaat cggcacctga
      841 agagaaatcg ttaagtgaaa aaccgaccga ttggatattg taaaggcagc cttcgagggc
      901 tgccgccatt gtgcctgaat agaggacact taccgaagca tttgaaccat gatttatgcc
      961 tgaaagaagc cagtcgggca tgcgctccgt gagctggttt attgcaagtt ttacactgtc
     1021 ggtgggagta ccgttacagg tgtatatccg gtgtttttca ttttcttcca gcagtttgac
     1081 acgaataggg ttttttaccg tcagtgcctg cgacatgccc gacatacttt ccatggtcga
     1141 aatgagaaca acctttccga attcttcggc tatttcaagc aatgtacgca atcctgcagc
     1201 atacaatcca tcatcgtttg ttatgagaat aagtctatcg ccctgttcaa caaccatatt
     1261 aagctatttg catgcaaata tattcaaatc ctgatgactc acagaataaa cttttaccaa
     1321 atcctttatc tttttttaaa attcttactt ttgcaatctt gtaaaacatt atttcaattt
     1381 ttgagatgaa aatttcatac aactggctaa aagagtattt acagatagac cttgatccac
     1441 acgatgttgc agaaatactt accaatacag ggcacgaaat agaagagact gaggaatggt
     1501 attctgttga aggcggttta aagggaatag tgattggaga agttttgtcg tcgcagaaac
     1561 atcccgatgc cgacagatta actgtgaata tagtggacgt tggcggtgag aggccactta
     1621 ctattgtatg cggtgcacct aatgtggcag cagggcaaaa agtacctgta gcgctgccag
     1681 gttcattcat attcaaaggt gatgaaaaaa ttgagataaa gacatcaaga ataagggggc
     1741 agatttcgga gggaatgata tgtgccgaag atgaaatcgg tatgggcaca tcgcatgaag
     1801 gtattatggt tctttcagct gatgcaaaac cgggaactcc agccgcagaa tatttcggag
     1861 ttgaaaaaga ttttgtcttt actattggtt tcacccccaa ccgtatcgac tgtgcttccc
     1921 atattggact ggcacgtgac cttgctgcat atcttgctgt gaacaggggc atggacatca
     1981 gacttaagat gccttcaatt gaaagtttta aaccggaaaa cagccgggaa actttcgaag
     2041 taataattga aaatactaga gactgtccga gatataccgg tgttaatata tttgacgtaa
     2101 aagtagctga atcgccgcaa tggctgaaaa acaggctcag ggctgtcggg ctaagtccga
     2161 taaacaacgt ggtggacata acaaactatg tgctgtatga atgcgggcag ccgttgcatg
     2221 ctttcgatgc tgatatgata acaggtcaga aagttattat aaaaaacctg cccgatggta
     2281 caaaaatgac tacactcgat aatgttgaac gggtattatc tgctaaagat ctcataatat
     2341 gcaatgaatc tgaaggtatg tgtattgcag gcgttttcgg aggtctgaag tcgggagtta
     2401 cagaagcaac cagaaatgtt tttctcgaaa gtgcatattt caatccggta tccatcagga
     2461 aaactgctaa aaggcatagc ctgaatactg atgcatcttt caggttcgaa agaggtgcag
     2521 atccggagat cacggtatgg gcattgaaaa gagctgccac tcttatcagg gatatagccg
     2581 gtggaaaact acttgcagat atacgcgacg tctatccggt aaaaataccg aaccctgtaa
     2641 taactatatc atgcgacagg ataaaccggc ttattggcaa agacctgcca tacgataaaa
     2701 taaagaaaat tatttccctg atggacatta aaatcctttc tgaaaatctt ccggaaatga
     2761 aaattgagat acctgcccgg aaagttgacg ttcagaagga ggctgatgta actgaagaga
     2821 tactcaggat ttacggatac gacaatgttg aagtcgggaa tcacgttaat tcaacacttt
     2881 cctatactga aaaacccgac aggcacaagc tgatgaacct ggtttccgag atgctctcgg
     2941 ctaatggttt ttcagaaata atgtgaaatt cgcttgtacc tgcgtcatgg ttcgaaaaca
     3001 atcccgattt cgactcaaca aaacttgtta agctggccaa tccgctcagt tccgatctga
     3061 atgccatgag gcaatcatta cttcccgggg gactgagcac catagcaaga aacataaaca
     3121 ggcagaatta tgatttgaaa ctttttgaat ttggtaattg ttactttttt aataatacta
     3181 ccggagtcaa gcgagttgac aggtattctg agagaatgag ccttgacctt tttatgacag
     3241 gtaacagggc aaagcatacc tggaactcac caaaaactcc tgccgacttt ttctatatga
     3301 aatcttttac cgagatggtt ctccagagga caggcctcga tgctgactca cttaaacagg
     3361 cagaaagcga aaaaagttat tttaccgaat cacttgtata tcttgttaat ggcaaacctg
     3421 ttgccgaaac tggtaaaatt tcgccttctt accttaagaa attcgagatt gagcaggacg
     3481 tttattacag tcacattgat tgggattacc tgctggaagt gataaaaaac aatgctataa
     3541 cctacaggga gctgc
//
LOCUS       DAOSNB010000273         3550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26434_length_3550_cov_3.429757, whole genome shotgun sequence.
ACCESSION   DAOSNB010000273 DAOSNB010000000
VERSION     DAOSNB010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26434_length_3550_cov_3.429757"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLG42_09750"
     CDS             <1..454
                     /locus_tag="PLG42_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU31532.1"
                     /translation="WIYGKKVVLDAGDSQTLRPGMIITARKLRDENSLLKRRDMKTVT
                     ARDAVPATSCQVLQGITKAALQTTSFFSAASFQETTKVLSEAAILGKVDRLEGLKENV
                     IVGHLIPAGTGVREYGSIIVGSKEDFEKLAAEREPKMEPAEAKNNKRG"
     gene            457..774
                     /locus_tag="PLG42_09755"
     CDS             457..774
                     /locus_tag="PLG42_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOU31533.1"
                     /translation="MSEQKNPNQISIELNEEVAQGIYSNLAVITHSSSEFVIDFIRLV
                     PGIPKAQVKSRIILTPEHAKRLVAALQDNIAKYEAVYGVIKEVKDSGPIMPISFGGPT
                     AQA"
     gene            948..1343
                     /locus_tag="PLG42_09760"
     CDS             948..1343
                     /locus_tag="PLG42_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31534.1"
                     /translation="MGIKLHRLLAMSLIILPLFFNSECKKQPRCGCGGDVIFTLTDAQ
                     VLVQYNESGTNIMFSNPINPMATYYFCNPSKWIDTIKKMNTKEYLLLSGEVYYECNYM
                     YMSGNYYTYTPPVYQVIVTSVREDNYGKK"
     gene            1444..2226
                     /locus_tag="PLG42_09765"
     CDS             1444..2226
                     /locus_tag="PLG42_09765"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOU31535.1"
                     /translation="MHFYRISFAGAGKVAGALSREFFSKGHKIIQVASAGNKRGPALA
                     ELFGSEWLDSLNFTSPADFIIVAVPDNLLGEVLHKIKCPSGTIVVHTAGSYGLEVFPE
                     NIRYRGVFYPLQTFSEGRKISFNEIPVFLEASDSNVMSGLKELASAITSRIYESVAEH
                     RRMLHLAAVFACNFTNHMLTLAKQICSKTPYNFNVMLPLINETVNKAIEKGPELSQTG
                     PAIRNDTLTIKKHLELLSFSEEMKRIYLEITNSISEFYKTNE"
     gene            2219..2740
                     /locus_tag="PLG42_09770"
     CDS             2219..2740
                     /locus_tag="PLG42_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOU31536.1"
                     /translation="MNNYKEKLAAIKGFVFDIDGVLSLQTIGLDKSGVPNRTVNLRDG
                     YALQLAVKKGYKIGVISGASPKGYLKRLRQLGVRDVYFNSKSKVVHFREFLKKNGLNA
                     SEVVYMGDDIPDCEIMKEAGVPVCPSDADSEIKELAVYISDKKGGEGCVRDIIEQVMR
                     LQNKWMDTDAFSW"
     gene            2724..>3550
                     /locus_tag="PLG42_09775"
     CDS             2724..>3550
                     /locus_tag="PLG42_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOU31537.1"
                     /translation="MPLAGEKMKEFLKLIRFQNLLIVALTMILMRYAIISPILSRVEV
                     SLYSIAGNAKTMILQLPWYDFAVLVLATIFITAAGYVINDYFDVRTDLINKGRVIVGT
                     AIPRKKALMWHNIFNTLGVAGGFYVSWRIGFFWAGIMFLLVTGLFYFYSATYKRQFLL
                     GNLMVAILTAMLPLLVLFYEWIPLRDYYAVNAIRMPDFRMIIWWIGGFSLFAFLTTLT
                     REIIKDIEDFEGDTAYGRNTLPVVLGIRASKLVAITLVILTLIMIYLIWYFFINDLIT
                     "
BASE COUNT         1074 a          660 c          808 g         1008 t
ORIGIN      
        1 ctggatctat ggcaagaagg tggttctgga tgcaggtgat tcccagacgc ttcgtcctgg
       61 catgataatt acagcacgta agctgcgtga cgagaattca ctgctcaaga ggcgtgatat
      121 gaagacggtt acggcaaggg atgccgttcc tgcaacttca tgccaggttc ttcaaggtat
      181 caccaaagct gcccttcaga ctacaagttt cttctccgct gcatcattcc aggagacaac
      241 aaaagtgctc agcgaagcag ccatactcgg taaagttgac aggcttgagg gtctgaaaga
      301 gaacgttatc gtcgggcacc ttattccggc aggaacaggc gtcagagaat acggcagcat
      361 aattgtcggt tcgaaagagg attttgaaaa actggcagcc gaaagagaac ctaaaatgga
      421 acctgctgaa gcaaaaaaca ataaacgagg ataaaaatgt cagaacagaa aaaccctaac
      481 cagatcagta ttgaactgaa cgaggaagtg gcacagggga tctattcgaa cctggcagtt
      541 ataactcatt cctcgtcgga gtttgtgatt gattttatca gacttgtgcc gggcattccg
      601 aaggctcagg tcaaatcaag aatcatcctt acacctgaac acgccaaaag actcgttgca
      661 gcattgcagg ataacattgc taaatatgag gcggtttatg gtgtgataaa agaggtaaaa
      721 gactcaggcc cgataatgcc catcagcttt ggagggccaa ctgcccaggc ataagatatc
      781 agtcatgcaa cagcacagaa gctgtctttg agtttaagtt ccggaactgc ggggagacaa
      841 agaggggaaa aatttaagac agtttctgtg cttgtttttt gtcgtgtatg taataaaatc
      901 ttaattttga agttaaatta aagaacgggg aattgaagtt tactgatatg ggaattaaat
      961 tacacagact tctggcaatg agtctcataa tcctgccgct tttttttaac agcgaatgca
     1021 agaaacaacc gcgttgcggg tgcgggggtg atgttatttt cactcttaca gatgctcagg
     1081 ttctggtgca gtacaacgaa agcggcacaa atatcatgtt cagtaacccc ataaacccga
     1141 tggcaactta ctatttctgc aatccgagca agtggattga tacaattaag aaaatgaata
     1201 caaaagaata tcttcttctg agcggagaag tctattacga atgtaattac atgtacatga
     1261 gcggtaacta ttatacctat actccgcctg tttaccaggt tattgtcacc tcggtgagag
     1321 aggataacta cggcaagaag taatcttatt tccccttttt cataaaattc tctggatatc
     1381 acatctgtaa ttgcatgtaa atatcttttt cctgcttttt tgtatctttt cagaaaaaat
     1441 aaaatgcatt tctacagaat ttcttttgca ggagcaggga aagtggcagg agcattaagc
     1501 agagagttct tctcaaaggg acataaaata atacaggtag catcggcagg aaataagagg
     1561 ggcccagcat tggctgaact ttttggctct gaatggctcg atagcctgaa tttcacttcg
     1621 cctgctgatt ttattattgt ggctgttcct gataatttgc tgggggaagt gctccataaa
     1681 atcaaatgcc cttccggtac aattgtcgtt cacacagccg gtagttacgg cctcgaggtt
     1741 tttccggaaa atatcagata cagaggtgtt ttctatcctt tacagacctt ttctgaagga
     1801 aggaaaataa gttttaatga gattccagtc tttctggaag catctgacag caatgttatg
     1861 tcggggttga aggaacttgc atcggcaatt acttcgagga tttatgaatc cgttgccgaa
     1921 caccgaagga tgctccatct ggcagcggtt ttcgcatgca atttcaccaa ccatatgcta
     1981 acccttgcaa agcagatatg cagcaaaaca ccctacaatt ttaatgttat gttgcctcta
     2041 ataaatgaaa ctgtgaataa agcaattgaa aaaggccctg aactttccca gaccggtcct
     2101 gcaataagaa atgatacgct tactattaaa aagcatctgg aattattatc tttctcggaa
     2161 gaaatgaaga gaatatattt agaaataact aattcaatat cagaatttta taaaacaaat
     2221 gaataattac aaagaaaaac ttgccgcaat aaaagggttt gtttttgata ttgacggtgt
     2281 gctgtcgtta cagacaattg gtttagataa gtcaggagtt ccgaacagga cagtaaacct
     2341 gcgcgatgga tatgctcttc agcttgccgt caaaaagggt tataagatag gtgttatatc
     2401 aggagcaagt ccgaaagggt atttgaaaag acttaggcag ctgggagtca gagatgtata
     2461 ttttaacagc aaatcgaagg tagttcattt cagggaattt ctgaagaaaa acggacttaa
     2521 cgcctccgaa gtggtttaca tgggcgatga tatacccgac tgcgagatta tgaaagaggc
     2581 cggtgtgccc gtatgccctt cggatgccga cagcgaaatc aaggagcttg cagtttatat
     2641 atctgacaaa aaaggcggcg agggatgcgt gagggatatt attgaacagg ttatgaggct
     2701 acagaacaaa tggatggata cagatgcctt tagctggtga aaaaatgaag gaattcttga
     2761 agctcatacg gtttcaaaac ctgctcattg ttgcgcttac aatgatactg atgcgctatg
     2821 caattataag tcccattcta agtagagtag aagtttctct atattcaata gccggaaatg
     2881 caaaaaccat gatactgcaa ctgccatggt atgactttgc tgttctggtt ctggcaacaa
     2941 tatttattac tgcagcaggt tatgtgataa acgattattt tgatgtcaga acagacctta
     3001 tcaataaggg aagggtaatt gtaggtactg cgatccctcg taaaaaggca ctcatgtggc
     3061 ataatatttt taatactctg ggtgttgcgg gagggtttta cgtatcatgg cggatcggat
     3121 ttttctgggc cgggattatg ttcctgcttg ttacaggtct gttttatttt tattctgcaa
     3181 cctacaagcg ccagtttctc ctgggaaatc tgatggtggc tattttaact gccatgttac
     3241 ctctactggt tttgttttac gaatggattc cgctgcgcga ttactatgca gttaatgcca
     3301 taagaatgcc cgattttaga atgataatat ggtggatagg aggtttttcg ctgtttgcat
     3361 tcctgactac cctgaccagg gaaataataa aagacataga agattttgaa ggtgacactg
     3421 catacggcag aaataccctt cctgttgtgc tcggtattcg tgcttcaaaa ttggtagcta
     3481 ttacacttgt aattttgact cttataatga tttacctgat atggtatttc tttataaatg
     3541 acttgataac
//
LOCUS       DAOSNB010000274         3541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26517_length_3541_cov_3.974756, whole genome shotgun sequence.
ACCESSION   DAOSNB010000274 DAOSNB010000000
VERSION     DAOSNB010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26517_length_3541_cov_3.974756"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1256)
                     /locus_tag="PLG42_09780"
     CDS             complement(51..1256)
                     /locus_tag="PLG42_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31538.1"
                     /translation="MKRRNFIKLTGTGSMALATSFYISSGQENRKIKAALIGAGWYGM
                     VISTAAIKAGDVEFIGVCDVDSDHLKTGADEIEKLQGSRPKTFKYYQEMLDMKDLDAV
                     FIGTIPHWHALQFIDACKRGLHIYCEKPLSYDVAEGIAMVNAWKRAGNIVQIGFQRRQ
                     SMAFKKAKELIESGRIGEVRQIVAQIHYNPGQQDTRIQPPPPSLDWEEWCGPAPKLEY
                     RPSIGHKAWRLEKEYGNGHLVDWGIHHIDIIRKIMGENMPSEFYTTGGIFVLKDQITT
                     PDTLTAHMKFNKVPVVWQHRLWGNGDVTPEFKNGIFFYGEKGTIFAEDNKIIIFSAEK
                     DLKREDISLPTPMMQDNHVRNFLDAVRAQNKNLVSCTPEDAFKSTATVQLAMISYYTD
                     SVVRWDQTR"
     gene            1436..2722
                     /locus_tag="PLG42_09785"
     CDS             1436..2722
                     /locus_tag="PLG42_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HOU31539.1"
                     /translation="MVKPAGSVCNLNCAYCYYLEKRKLYPEKKDFRMTEELLEEFTKQ
                     YIEANEVPVVTFTWQGGEPTLMGLEFFKRAIELQKKYAGRKRIENAFQTNGTTLNDDW
                     CKFFKDNNILVGISIDGPEHNHDHYRKTYSGGPTFRRVMKGIELLQKHSVEFNTLSVV
                     NDYNVHFAKETYRFLKDIGSIFMQFLPVVERVAEGIKDDSLKLVSNEYGNRTTVAPWT
                     VNGKDYGKFLITIFDEWVRNDVARYFVQIFDATLANWVGEMPGVCVFAGTCGDALVME
                     HNGDVYSCDHYVYPENFLGNILEKPLIEMARSQEQFDFGMKKRNNLPRQCMQCDVLFA
                     CHGECPKHRIAITEDGEYGLNYLCEGYKMFYRHVTPYMDFMAKELQNKRAPANVMQWI
                     KNRESRVVKPVIPERNDPCPCGSGKKYKNCCGKGLL"
     gene            complement(2739..3209)
                     /locus_tag="PLG42_09790"
     CDS             complement(2739..3209)
                     /locus_tag="PLG42_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31540.1"
                     /translation="MKKLIIILLTLSISGLSFVYGQMAVGYNTDGNTLLLSTSPLKTL
                     WGEFRINTKAYNQASWTYSDRGITQLYLSARVFNAQNVIFYAGGGLGANLLSEDKDKW
                     LSINIPAGIRIQPFTKYPGLLIIGEYNPMFVVEEDVPIIHSVSAGLRILLIKGE"
     gene            complement(3212..>3541)
                     /locus_tag="PLG42_09795"
     CDS             complement(3212..>3541)
                     /locus_tag="PLG42_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31541.1"
                     /translation="VEDFSLSSAFETNKVYSFENGNWHLLNKNENPPLIHFSTRVIAD
                     KRNFCWLASYDNKKHEAVIYVYNGKSWLKSPDNSFPDDIITTIETDDENNIYIGTYKN
                     GIFILNQ"
BASE COUNT         1050 a          675 c          721 g         1095 t
ORIGIN      
        1 gttggtgtcc caaatgcagc tgccttttca tttacacaaa attctggaca ctacctcgtt
       61 tgatcccatc tgacaactga atcagtataa taggaaatca ttgcaagctg gactgttgct
      121 gtagatttga atgcatcttc aggcgtgcag cttacaaggt ttttgttttg ggccctaacc
      181 gcatcaagaa aattcctcac atggttatcc tgcatcatag gtgttgggag tgatatatcc
      241 tctcttttta aatctttttc tgctgaaaaa atgatgattt tattgtcttc agcgaatata
      301 gttccctttt cgccataaaa gaatattccg tttttgaatt ccggtgttac atcaccattt
      361 ccccataggc ggtgttgcca tacaaccggc actttattaa atttcatatg tgcagttagt
      421 gtatcaggag tggtaatctg gtctttcaga acaaatattc cgcctgttgt atagaattcg
      481 gatggcatat tctctcccat tatttttctt attatgtcaa tatgatgtat accccagtca
      541 accaggtgtc catttccata ttccttttct agtctccatg ctttatgacc aatgcttggt
      601 ctgtattcaa gtttcggtgc aggtccgcac cactcttccc agtcgagtga aggaggcgga
      661 ggttgaatcc ttgtatcctg ctgaccagga ttatagtgaa tttgagcaac aatctgcctc
      721 acctctccta tcctacccga ctcaataagt tcttttgcct tcttaaaagc catgctctgc
      781 ctgcgttgaa aacctatctg aactatattt ccagctcttt tccatgcatt aaccatagca
      841 attccttcag ccacatcgta cgacagaggt ttttcgcaat agatatgcag gcctctttta
      901 caagcatcaa taaactgaag tgcatgccag tgtggtattg ttccaataaa tacagcatca
      961 agatctttca tgtcaagcat ttcctgataa tacttgaatg tcttaggtct tgaaccctga
     1021 agcttttcaa tctcatcagc accagttttt agatgatcag aatcaacatc acaaacacct
     1081 ataaattcaa catcccctgc tttaatggct gctgtagaaa taaccattcc gtaccagcca
     1141 gcaccaataa gtgcagcttt aatctttcta ttttcctgtc cggaagaaat atagaatgat
     1201 gttgccagtg ccatagaacc tgtgcctgtt aactttatga aatttcttct tttcatttca
     1261 aaatttttta tacagataaa tttaacaatt atggatgata aaaataggtc acaagtcagc
     1321 aattttgata atttttgcag tttaagcaaa gaatgttatg ccagcagaaa gaagaaaaac
     1381 tgccatactc tttacagatg cacttaaact taaacgacca gctgctttta acgttatggt
     1441 caagccagca ggttcagttt gcaatcttaa ctgtgcatat tgttattatc tagaaaagag
     1501 aaaattatat ccggaaaaaa aagatttccg gatgactgag gaattacttg aagagtttac
     1561 aaagcagtat attgaggcaa atgaagtacc tgtagtaact ttcacatggc agggtggtga
     1621 gccaacgctt atggggcttg aatttttcaa aagggccata gagcttcaaa agaaatacgc
     1681 cggaagaaag cggatagaaa atgcttttca gacaaatggt acaacgttga acgatgactg
     1741 gtgtaagttt ttcaaagaca acaatatact ggtaggtatc tcaatagatg gtcctgaaca
     1801 caatcacgat cattaccgga agacctattc gggagggcct acattcagaa gggttatgaa
     1861 gggcatagag ttacttcaga agcacagtgt tgaatttaac accctgagtg ttgtaaatga
     1921 ttacaatgtt catttcgcaa aggagacata caggttttta aaagatattg gcagtatatt
     1981 catgcagttt ctcccggttg ttgaaagggt agctgaaggt attaaagatg attctcttaa
     2041 gcttgtctcg aacgaatatg ggaaccggac aactgttgca ccctggaccg ttaacgggaa
     2101 agattacgga aaatttctga taacgatttt tgacgagtgg gtgagaaatg acgttgcaag
     2161 gtattttgta cagatttttg atgccaccct ggcaaactgg gtgggtgaaa tgcccggggt
     2221 gtgcgtattt gctggaacat gcggtgatgc gctggtgatg gagcataacg gagatgttta
     2281 ttcatgcgac cattatgtat atcctgagaa ttttcttggt aacatacttg agaagccatt
     2341 gattgagatg gccaggtcgc aggagcaatt tgatttcggg atgaagaaac gtaataatct
     2401 cccccgccag tgtatgcaat gtgatgttct ttttgcatgc catggggaat gtccgaaaca
     2461 caggatagcc ataactgaag acggggagta tggattaaac tatctgtgcg agggatataa
     2521 gatgttttat cgtcatgtta cgccctacat ggatttcatg gcaaaagaac tgcaaaacaa
     2581 aagagcgcct gcaaatgtaa tgcaatggat aaagaaccgg gaaagcaggg ttgtaaagcc
     2641 tgtaattcct gaaagaaatg atccctgccc gtgcggtagc ggaaagaagt ataaaaactg
     2701 ttgtggaaaa ggcttgctat gagaagaacg tatcagtatt attcaccttt tatcagaagt
     2761 attcttaaac cggctgatac cgagtgaatt atcggcacat cttcttccac aacaaacatc
     2821 ggattatatt caccgataat aagaagtcca gggtattttg tgaagggttg aattcttata
     2881 ccagcgggta tatttatact cagccattta tccttgtctt ccgacaggag atttgccccc
     2941 agtccgcctc ctgcatagaa tataacattc tgggcattga acaccctggc tgaaagatac
     3001 agctgagtaa tgcccctgtc gctgtatgtc caggatgcct ggttatatgc ttttgtattg
     3061 attctgaact caccccaaag tgttttcaga gggcttgtcg acaataataa agtatttcca
     3121 tcagtattgt aaccaacagc catttgtccg taaacaaaag atagacctga aattgaaagt
     3181 gtcagcagaa taattattaa ttttttcata atcattgatt taatataaat ataccatttt
     3241 tataagttcc gatgtaaata ttgttttcgt catcagtttc aattgttgtt attatgtcat
     3301 ccggaaagct gttatctggt gatttgagcc aggattttcc gttatagaca taaattacag
     3361 cttcatgttt tttgttgtcg taagaagcca gccagcagaa attcctttta tcggctatta
     3421 cacgtgtaga gaaatggata aggggaggat tttcgttttt gttcagtaaa tgccagttac
     3481 cattttcaaa ggaatatacc ttatttgttt caaaagcaga ggatagtgaa aaatcttcaa
     3541 c
//
LOCUS       DAOSNB010000275         3525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26666_length_3525_cov_5.146686, whole genome shotgun sequence.
ACCESSION   DAOSNB010000275 DAOSNB010000000
VERSION     DAOSNB010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26666_length_3525_cov_5.146686"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..1677)
                     /locus_tag="PLG42_09800"
     CDS             complement(466..1677)
                     /locus_tag="PLG42_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31542.1"
                     /translation="MIFIFTGCGNESKKTEKKSSMVISDTTALYGYNKKFLEKYTGTI
                     ELSSGNSAILIVPAWQGRVMTSTAEGDSGFSFGWINHQLISSGQLQKHINPFGGEERL
                     WLGPEGGQYSIFFPQGSEFTYENWQTPALIDTEPFEVVEKSGTSVLFRKDVSLTNYSG
                     FKFDLRIERRVSIVSPEKIPQLSSINLKGLNCVAYSSENTLINTGQEEWKKESGLLSI
                     WMLGMFTPSPSVVVVIPVKEGDEELIGPKVNDNYFGKISSDRLKVVGNHIFFRADGKS
                     RGKIGIPPLRAAGVMGSYDSENNILNLLFCDVPEGEIDYVNSAWELQENPYSGDAYNS
                     YNDGPLEDGSQMGPFYELETSSPAAKLKPGGSITHSQITVHLTGAKEKLNIVAEKILG
                     VDLETISSIFN"
     gene            1944..>3525
                     /locus_tag="PLG42_09805"
     CDS             1944..>3525
                     /locus_tag="PLG42_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOU31543.1"
                     /translation="MKNLVLILIIPLFFLVGCSGGNGGKNEKNVYAIIPAPLSLSEMP
                     GKFIFREKSTIVLSPLNESTRLSADFLASLVKNATGYELQIKEGSKAPNGSVLMAVDT
                     SVKNPEGYTLTISKGKIVVKAGSPVGMFYAVQTIRQLMPPEVEKPFSGQAVTLSVPCC
                     EIKDEPRFVYRGMHLDVGRHMFPVETIKRYIDLLAMHKFNYFHWHLTEDQGWRIEIKK
                     YPKLTEVGAFRKETIVGHAGKKPQVFDGKPYGGFYTQNEVREIVKYAASKFITVIPEI
                     EMPGHSLAALASYPELSCTGGPFEVGTKWGIFEDVYCAGKEETFKFLEDVLTEVMELF
                     PSKYIHIGGDECPKTRWKKCPLCQKRIKQEGLKDENELQSYFIRRIEKFLVSKGRKLI
                     GWDEILEGGLAPEATVMSWRGVAGGIEAAKQNHDVIMTPTRYFYLDYYQTEPDNEPLA
                     IGGYLPLETVYSFDPRFEELTEEQHKHILGVQGNVWTEYISTPEHLEYMACPRMFAIS
                     EVGWTQASKKDFEDFLARF"
BASE COUNT         1021 a          721 c          786 g          997 t
ORIGIN      
        1 cctttgttgt ttacaggccg gaaagcgagc catgcttgca tgagcgagcg tacggactgt
       61 aaactgacaa gattcagcgt cagctgaatt ttgcctgccg gggcgagggt ttcacaaaag
      121 ggatcaccgg aggtaatccc cgggtgttat taaagggctg aagcaattca gccctttgtt
      181 gtttacaggc cggaaagcga gccatgcttg catgagcgag cgtacggact gtaaactgac
      241 aagattcagc gtcagctgaa ttttgcctgc cggggcgagg gtttcacaaa agggatcacc
      301 ggaggtaatc cccgggtgtt attaaagggc tgaagcaatt cagccctttg ttgtttacag
      361 gcaatttagg gctgtaacaa aggtctcttt ttgttttcca ggctgaatac cggggtgaac
      421 tacattgtcc ttcccgggga gctgggagtg atcaatatca agttttcaat tgaaaatgct
      481 gctgatagtt tccagatcaa ccccaagaat cttttccgcc actatgttaa gcttctcctt
      541 tgctccagta agatgtactg taatctgcga atgggtaata ctgcctccag gtttcaactt
      601 tgcagccggt gatgatgtct cgagttcata aaacggaccc atctgcgagc catcttcaag
      661 cggaccatca ttgtacgagt tgtacgcatc acccgaatac ggattttcct gtaactccca
      721 cgccgaattt acataatcaa tctccccttc aggcacatcg cagaaaagca gattcagtat
      781 gttgttctcc gaatcataac tgcccatcac cccggcagct ctcagcggag gtatccctat
      841 ctttcctctg ctctttccgt cagccctgaa aaatatatga ttaccaacca ctttcaacct
      901 gtcggatgaa atctttccga aataattgtc attgaccttt ggtcctataa gttcttcatc
      961 tccttctttt acaggtatta ccacaacaac agaaggggag ggggtgaaca ttcccagcat
     1021 ccatattgat agtagtccgc tctctttctt ccattcttcc tgtcctgtgt ttatgagcgt
     1081 attttccgaa ctatatgcaa cacaattgag acccttcagg ttgatagaag ataattgagg
     1141 aattttttca ggcgaaacaa ttgatactct cctctctatc ctgagatcaa atttgaatcc
     1201 gctgtagttt gtaaggctaa catctttccg gaataacaca ctggttccag atttctccac
     1261 tacctcgaat ggttctgtgt caatcagcgc gggtgtttgc cagttttcat aagtaaattc
     1321 gctgccctga ggaaaaaata ttgaatactg acctccttcc ggaccaagcc atagccgctc
     1381 ctctccacca aaaggattaa tatgtttctg aagttgacct gaggaaataa gttggtggtt
     1441 aatccagcca aaactgaaac cactgtcacc ttcagccgta gaggtcatta cccgcccctg
     1501 ccaggcagga acaatcaata ttgctgagtt tccggatgag agttctattg ttccagtata
     1561 tttttccaga aactttttat tgtacccata taaggctgta gtgtcgctta taaccatgga
     1621 tgattttttt tcagtttttt tactttcatt accgcaaccg gtaaagataa aaatcataag
     1681 caaaataaaa cctgtaattc tgtaattaga catattttat ataaattatt gatgttcaat
     1741 tacatattga tatataccca taaaaatatt ggaggtaatg aagtttattc tttattcaaa
     1801 attaggaaac ctttatggga aatgaaatta tttgaatatg aacctgcgga aaaaatatca
     1861 tttataattt aagtttactg tatatttgaa gagtcaaacc ggtgtttatt attaatttcg
     1921 tgtaacaatt aaaatttcag cttatgaaga atctggtttt aattttgata attccgttat
     1981 tttttcttgt gggttgtagc ggcggaaacg gaggaaagaa tgagaaaaac gtctatgcaa
     2041 ttattcctgc tccgctgtcg ctcagtgaaa tgcccggaaa gtttattttc agggagaaaa
     2101 gcaccatagt tctttctcct ttgaacgaat caaccaggct ttcggcagat tttcttgctt
     2161 ctctggtgaa gaatgcaaca ggatacgaac tccagataaa ggagggaagc aaagctccga
     2221 atggttcggt attgatggca gttgacactt cggtcaaaaa ccccgaagga tatactctta
     2281 cgataagtaa agggaaaata gtggtaaagg ccgggagtcc agttggcatg ttttatgcag
     2341 ttcagactat caggcagctg atgccacctg aggtggaaaa acccttttcc ggacaggctg
     2401 taactctttc tgtgccgtgt tgtgaaatta aagatgagcc ccgtttcgtg tacaggggta
     2461 tgcatcttga cgtcggacgt catatgtttc ctgtcgaaac gataaaaagg tacattgatt
     2521 tgcttgcaat gcacaagttc aactatttcc actggcacct caccgaagac cagggatgga
     2581 gaattgaaat taagaaatat ccaaagctca ctgaggtagg agcattcaga aaagaaacca
     2641 tagtggggca tgcaggcaaa aagcctcagg tttttgatgg aaagccttac ggaggttttt
     2701 atacacagaa cgaggtgcgg gagatagtca aatatgcagc atcgaaattc ataacagtga
     2761 ttccggaaat tgaaatgccg ggtcactccc ttgcagcact tgcttcatat ccggagcttt
     2821 catgtaccgg aggaccattt gaagtgggta caaaatgggg aatatttgaa gatgtttatt
     2881 gcgcaggtaa agaagaaacc ttcaaattcc tggaggacgt ccttaccgaa gttatggaac
     2941 tttttccgtc caaatatata cacataggtg gtgatgagtg tccaaaaaca cgatggaaga
     3001 agtgtcctct ctgtcagaaa agaattaaac aggaaggact gaaggatgaa aatgaacttc
     3061 agagttactt tatcaggaga atagaaaagt ttctggtatc caaaggcagg aaactcatag
     3121 gctgggacga aatactcgaa ggaggcctgg cccccgaggc aactgtaatg tcgtggagag
     3181 gtgtagcagg tgggatagaa gctgcaaagc agaatcatga tgtgattatg acacccacac
     3241 gttatttcta cctcgattat tatcagactg aacccgataa tgagccgctt gcaataggcg
     3301 ggtacctgcc actcgaaact gtctattcgt ttgatccgag atttgaggaa ctgacggaag
     3361 aacagcataa acatattctt ggagtgcagg gaaatgtatg gactgagtat atttccactc
     3421 ccgagcatct ggaatatatg gcatgtccga gaatgtttgc cataagtgaa gtaggatgga
     3481 cacaggcatc aaagaaagac tttgaggatt tccttgccag attca
//
LOCUS       DAOSNB010000276         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_26755_length_3516_cov_4.629876, whole genome shotgun sequence.
ACCESSION   DAOSNB010000276 DAOSNB010000000
VERSION     DAOSNB010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26755_length_3516_cov_4.629876"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1444)
                     /gene="mnmE"
                     /locus_tag="PLG42_09810"
     CDS             complement(41..1444)
                     /gene="mnmE"
                     /locus_tag="PLG42_09810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOU31544.1"
                     /translation="MRKGETICAPATATGGAIAVVRVSGPKSFEICEKIFRPHNSKTR
                     LRERRGHTVVYGEIVDGEEIIDDVLVTVFRKPHSYTGEDSVEISCHGSQYIIRKVLEL
                     LINNGAVPARPGEFTMRAFMNGKMDLSQAEAVADLVASSSEASHRLAIHQMRGGFSEE
                     IMRLRQELLHFTSLIELELDFGEEDVEFADRSELLKIVRRVKELAGKLASSFRLGNAI
                     KNGVPVVIVGKPNSGKSTLLNALLMEERAIVSDIPGTTRDFIEDTIVIDGIEYRFIDT
                     AGLRETTDAIEILGIKKTLEKIKQARVILLVDEITDSPETINRRADYLRKLAGDDEKR
                     LIILINKTDLYPEKMSRKELGKSIMLRDNESLLFISAKGKIGLDELRAKLGEVIEKEK
                     LSSEDVIISNMRHYEALSKVSESLARVEEGLESGIPEDLIAEDIRQAIHYLGEITGEI
                     TSDEILGNIFKNFCIGK"
     gene            complement(1551..2321)
                     /locus_tag="PLG42_09815"
     CDS             complement(1551..2321)
                     /locus_tag="PLG42_09815"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOU31545.1"
                     /translation="MKKSMLVNLLLLSLLRISAQQPDAGQIMEKSRELTLTGSLKANV
                     SLTITEKNGSTRNRTISMITKSYTDGTEKRLIKFLEPADVKGTGMLIIDYKTAADEMW
                     IYLPALKKTRRIVSSEKGKSFMNSEFSNADMSSPSLSDFKYRHLEGSGQDDVWIIESV
                     PADNEKVAEYGFSRRISYIGKSNYQVKKMEFFDRENKLFKIIEIASVFPGENGKYIVS
                     EMKAVNLSNGRSSVIKLTNITLNSGAEDSLFTLQNLER"
     gene            complement(2254..>3516)
                     /locus_tag="PLG42_09820"
     CDS             complement(2254..>3516)
                     /locus_tag="PLG42_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOU31546.1"
                     /translation="KTDYSAKLRMNFVNHGMDGFFRRLSMLITKHPGKVLVFSVIVIL
                     SVGAGIIFLRTDTNQEHFFPERHPLRRTSEIINKSFGGSQTISVMISGNIKDPRVMKG
                     IDLLTNRIESEKGVGKVFSISKAVREMSKALYDRDEEGYNRIPDSEEAIAQMFELYYM
                     SGDAEDFKQLLNPENDRAHIIIRLSNPSGDVIKRIVNIVEETRKEIPAKITTGGYALI
                     MYDFTESLIKGQVFSLAFALIIVFILLSLVFRSVKGGLTGSIPLAASIIVLFGFMGYS
                     GIAIDPATALLSSVMIGVGVDFTIQYMWRFNIELKKYKDYFKATEKSFESIGQSIVIN
                     AFAVMGGFGALIFSGFTSIKFFGYLVVISIASCLAGSLFVIPAFLIKFRPRFIEKNLS
                     KIKYSEYEKEYAGKSVASVIVKDIGPAA"
BASE COUNT         1022 a          794 c          565 g         1135 t
ORIGIN      
        1 tcttgcactt aaaatcaata gtagtgaagt tttgttctga ttattttcca atacagaagt
       61 tcttaaatat atttccaaga atctcatccg acgtaatctc ccctgtaatc tcaccaaggt
      121 aatgaatagc ctgcctgata tcttctgcta tcagatcttc gggaatacca gattctaaac
      181 cttcttctac ccgggcaaga ctctcactaa cttttgataa agcttcgtaa tgtctcatat
      241 ttgaaataat tacatcttca gaactcaact tctctttttc tatcacttca ccaagttttg
      301 cccgtaattc atcaagccct atctttccct ttgcagaaat gaataacagc gattcattat
      361 cacgaagcat aatggattta ccaagctcct ttctggacat cttttcagga taaaggtcag
      421 ttttatttat aaggatgatc agtctctttt catcatctcc tgccagtttt cttaaataat
      481 cggccctccg gtttatcgtt tcaggagaat ctgtgatctc atccactaaa agaatgactc
      541 ttgcctgttt gattttttca agtgtctttt ttattccaag aatttctatg gcatcagttg
      601 tttccctcaa tcctgcagtg tcaataaacc tgtattcaat accgtcaatc actattgtgt
      661 cttctataaa gtcccttgtt gttccgggaa tatccgaaac tattgctctc tcttccatca
      721 gaagggcatt cagcaaagtg gatttacctg aatttggttt tcctactatt acaacaggta
      781 ccccgttttt tatagcattt cccagcctga aagatgaagc cagtttgccg gcaagttctt
      841 tgacccgcct tacaattttc agcaattcac tcctgtccgc aaattctaca tcctcctctc
      901 cgaaatcaag ctcgagctct atgagtgaag tgaaatgaag cagttcctgc cgcaatcgca
      961 ttatttcctc cgaaaaacct ccacgcatct gatgtatagc cagcctgtgg gatgcttcag
     1021 aagaacttgc aaccagatct gccacagctt cagcctgtga taaatccatc tttccgttca
     1081 tgaatgcacg cattgtaaac tctcccggac gagcaggaac agcaccatta ttaatcaata
     1141 attccagaac cttccggata atatactggg aaccatggca ggaaatctct acagagtctt
     1201 caccggtata tgaatgaggc ttacgaaaaa ctgttacaag cacatcgtca attatctctt
     1261 ctccatcaac gatttcacca taaactactg tatgacctct tcgttccctg agccttgttt
     1321 tgctattatg gggcctgaat attttctcac atatttcaaa actcttcggc ccgcttactc
     1381 ttacaacagc aattgcgccc cccgttgcgg tggcaggcgc acatattgtt tctccttttc
     1441 taatcacttt taatattcat ttgtaatgaa taagtaaaaa taataaatat agaggcaaac
     1501 aacataattt gctgccataa tttaagtgca gttgtgtaaa agtttatcta ttaccgttca
     1561 agattctgaa gcgtgaataa tgaatcttca gctcccgagt tcaaagtgat atttgttaat
     1621 ttaataacag aacttctgcc attcgacaga ttaacagcct tcatttcact aacaatatat
     1681 ttgccatttt ccccgggaaa aacagatgct atctcaataa ttttgaataa tttattttcc
     1741 cggtcaaaaa attccatctt ttttacctga taattgcttt ttcctatata acttatgcga
     1801 cgcgagaaac cgtattcggc aactttttca ttgtctgcag gaacagattc aattatccat
     1861 acatcatcct gccccgagcc ctcaaggtgt cggtatttga agtccgaaag agaaggggaa
     1921 ctcatatcgg catttgaaaa ttctgaattc ataaaactct ttcctttttc ggagctcaca
     1981 attcttctgg tctttttaag ggctggaaga taaatccaca tctcatctgc agccgttttg
     2041 taatcaatta tcaacatacc tgtacccttt acatcagcag gctcaagaaa cttaatcagc
     2101 ctcttttctg ttccgtcagt ataacttttt gtaatcatgg aaatggttcg attacgggtt
     2161 gaaccatttt tttcggtaat agtaagactt acatttgcct taagtgaacc tgtaagtgtg
     2221 agctctctgc ttttttccat tatctggcca gcatcaggct gctgggccga tatccttaac
     2281 aatgacagaa gcaacagatt taccagcata ctctttttca tattcactgt attttatttt
     2341 tgagaggttt ttctcaataa accggggcct gaattttatc agaaatgcag ggatgacaaa
     2401 caatgaaccg gcaagacacg atgcaataga gatcacaaca agataaccaa aaaacttaat
     2461 tgatgtaaat ccggaaaaga taagtgcacc aaagcctccc attactgcaa aagcattaat
     2521 aacaatactc tgacctattg attcaaatga tttctccgtc gctttgaaat aatctttgta
     2581 cttttttaat tcaatgttga acctccacat atactgaatg gtaaagtcaa ctccaacgcc
     2641 aatcataaca gatgagagga gtgcagtggc aggatcaatg gcaattcccg aataccccat
     2701 aaatccaaat agaacaataa tagaagctgc aagtggtatt gagcctgtaa gccctccttt
     2761 aaccgacctg aaaaccagag agagaagtat aaacactata ataagtgcaa atgcaagaga
     2821 gaaaacttgt ccttttataa gggattccgt gaaatcgtac attattaggg catacccacc
     2881 ggtagttatt tttgcaggaa tttctttcct ggtctcttca actatattga caatacgctt
     2941 tattacatca ccagatggat ttgatagcct tattataatg tgagccctgt cgttttcagg
     3001 atttaaaagc tgtttgaaat cctctgcatc cccactcatg taatagagtt cgaacatctg
     3061 agctattgcc tcctccgaat ctggaatcct gttatatcct tcttcatccc tatcatataa
     3121 cgccttactc atttccctta ctgcctttga tatagaaaat accttcccaa ctcctttttc
     3181 tgactctatc ctgttagtaa gaaggtctat tcctttcatt acccgggggt cctttatgtt
     3241 gccagaaatc attacagaga ttgtttgaga accaccgaaa cttttgttta ttatttccga
     3301 cgttcttctt aacggatgtc tttccgggaa aaaatgttcc tggtttgtat ctgtccggag
     3361 gaaaataatt ccagcaccta ctgataagat tactatcact gaaaaaacca gtaccttgcc
     3421 cgggtgtttt gtaataagca ttgaaagacg cctgaaaaaa ccatccattc catgattaac
     3481 aaaattcatc cttagttttg cagaatagtc tgtttt
//
LOCUS       DAOSNB010000277         3471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_27218_length_3471_cov_3.838700, whole genome shotgun sequence.
ACCESSION   DAOSNB010000277 DAOSNB010000000
VERSION     DAOSNB010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27218_length_3471_cov_3.838700"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..846
                     /locus_tag="PLG42_09825"
     CDS             79..846
                     /locus_tag="PLG42_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31547.1"
                     /translation="MKKLTIITLILTLFFTNIKAQTDTAKFKPHLITGIDFILHNGIW
                     GGTVPAQPIESSVNIGGTPFIAPGYGVIFNFRFMKNLSLYFDGNMYTRKTPVAYSGSY
                     ATSNWVAEQTNYSTDLIGPFDEEAYYKVQTTGFRLGLRGYLRHNKPVDPWVGLYWGYY
                     VVNHGVYNKNNSSTWGNAYNYVSGISLLNAGVDFWDKSKTLGLSLFIELGAPANRYYK
                     IENCLHSGWTFTDYGEGEPIFGYYRIGIALISCISKK"
     gene            complement(977..1915)
                     /locus_tag="PLG42_09830"
     CDS             complement(977..1915)
                     /locus_tag="PLG42_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOU31548.1"
                     /translation="MKKLSVLLFSMVSVLVYSVRSQTAEVLLRPAYVDLSSSDAPGAV
                     LVHLSGYSSDDVRYRLYNGSNQYYCWNAETGSFVSSNAYSSGPLITGTPITGTSFWIL
                     YKRGSNNSATATYRDRLGPEYSVNYQSVALPAATVIANSFTLSGKLSEDPAFPLTKKY
                     VILAWSGSALISASHTSLSTGEFLIACPTGITIEKIEARKINNEPAGAITGTWNNEAY
                     AGEIIPGVQTEVGHYLKDNIIIYPVPVHESLIVRNAESFKTIEIYNVAGKMISVCEVQ
                     DHNIVSIDFRHLPPGIYFIKLKATGKGTVVKKLIKS"
     gene            2157..2612
                     /locus_tag="PLG42_09835"
     CDS             2157..2612
                     /locus_tag="PLG42_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOU31549.1"
                     /translation="MEIKSPAFKEGELIPSRHTCDGHDVSPPLEWSGAPAETRSFALI
                     SDDPDAPVGTWVHWVIFNIPANVNKLEENIPKKEVLSNGARQGRNDFGRIGYGGPCPP
                     GGTHRYYFKLYALDRELDLKAGSTKRDLLKAMEGHVLAEAHLMGRYRRS"
     gene            complement(2760..>3471)
                     /locus_tag="PLG42_09840"
     CDS             complement(2760..>3471)
                     /locus_tag="PLG42_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylgalactosaminidase"
                     /protein_id="HOU31550.1"
                     /translation="LYPTHGLGPIAQVMNINRGDRMEYLTSMSTDDFTMYLREKELVE
                     QDPFYEPWRNAKFRGNFNTTLIRTAHGKTIKVQHDVSSSHPYSRIHLVKGTKGMAVKY
                     PRQMIAFGEEWVKDEAMQEIWDKYTPEIIKRVGEMARRVGGHGGMDFIMDWRLIDCLR
                     NGLPLDQDVYDGVTWSCVAPLSEWSVANRSRPIDVPDFTGGSWKTNKPHGINLETGGT
                     TQVLEVKRERESMQLNVR"
BASE COUNT         1058 a          732 c          666 g         1015 t
ORIGIN      
        1 actttcttta ttcatgattt catgaagaag caataaattt taatcaacaa caataatcat
       61 gaaataaaat tcagaactat gaaaaaatta acaatcatca cattaattct aactcttttt
      121 ttcaccaata tcaaagctca aacagatact gctaaattca agcctcattt gataacaggt
      181 attgatttta ttctgcacaa tggaatatgg ggtggaactg ttccagctca accgatagaa
      241 tcatctgtga acattggagg cacacctttt attgctcctg gatacggggt cattttcaat
      301 ttccgtttta tgaaaaacct ttccctttat tttgatggta atatgtatac cagaaaaact
      361 cctgttgcat attcaggaag ttatgccaca agcaattggg tagcagaaca aacaaactac
      421 agtacagatc tgattggacc ttttgacgag gaagcttatt acaaagtaca gacaacaggc
      481 ttcagacttg gattgcgtgg ttatttaagg cacaacaaac ctgttgatcc atgggttgga
      541 ttgtactggg gttattatgt tgttaaccat ggtgtatata ataaaaataa cagctcaacc
      601 tggggcaatg cgtacaatta tgtttcagga atttctctct taaatgctgg tgtagatttc
      661 tgggataaat caaaaacgct tggtctaagt ctattcattg aacttggcgc accagctaac
      721 agatattaca aaattgagaa ttgtttgcat tcgggatgga cttttacaga ttacggcgaa
      781 ggtgagccta tttttggata ttacaggata ggtattgcgt tgatatcttg catctcaaaa
      841 aaataaaatc accgttttca ggcagtaata ataacaaatt gtttacccaa tccaaatagc
      901 tgccaggcta ccaatgcacg aataaataaa attcgtgatt tcgtgtctac taacaaaaga
      961 agcctgatga atctatttaa ctcttaatca gcttctttac aaccgtaccc ttacccgtgg
     1021 ctttcagctt tatgaaatag attccgggtg gcagatgcct gaagtcaata gatacaatgt
     1081 tgtggtcctg cacctcgcaa acagaaatca tttttcctgc aacattataa atttcaatcg
     1141 ttttaaacga ctctgcattt cttacaataa ggctctcatg tacaggtacc ggataaatga
     1201 taatgttatc tttcaggtaa tgacctactt ctgtctgtac accgggtata atttcaccag
     1261 cataggcttc gttattccat gttcctgtta ttgcacctgc cggttcatta tttatttttc
     1321 tggcctcaat cttttcaatt gtaattccgg tgggacacgc aattaagaac tctcctgttg
     1381 aaaggctggt atgtgatgca gaaatgagcg ctgaacctga ccatgcaagt atgacatatt
     1441 tctttgtaag aggaaaagcc ggatcttctg acagttttcc tgaaagagta aagctgtttg
     1501 caattaccgt tgctgcagga agggcgacac tctggtaatt gacagaatac tcaggcccga
     1561 gtcggtcacg gtacgtggca gttgccgagt tgttggaacc tctcttatac aaaatccaga
     1621 aactcgtacc agttattggt gttccagtga taagaggacc cgatgaatag gcatttgacg
     1681 atacaaaact tccggtttca gcgttccagc aataatactg gttgctgccg ttataaagtc
     1741 tgtaccggac atcatctgac gagtaacctg aaagatgtac cagaacagct ccaggcgcat
     1801 cggaagaaga tagatcaaca tacgcagggc gtaataaaac ttcagcagtc tgagacctga
     1861 cggaatatac caaaacagaa accatgctga acaataatac tgataatttc ttcataatca
     1921 atacttttag cacgttatat acagcgagaa aactgtgaat ccaatttcgg caatattttt
     1981 ttcaaatcca acaattgtta gtaaaaatgt gctatcctca gtaataaaaa tattaacaaa
     2041 atgaaataaa aaaactacct tatctggcag aacatgaatg gtatcagatt tttttataat
     2101 tttataagac tctgagcatt tacaattcaa ataacaacat aaatgagcaa caagttatgg
     2161 aaatcaaaag tccggcattc aaagaaggtg agctgatacc ttcgaggcat acatgcgacg
     2221 gtcatgatgt ttcaccgccg cttgaatggt ctggtgcgcc tgcagaaacc aggagttttg
     2281 cactcataag tgatgatccc gacgctccgg tgggaacatg ggttcactgg gttatattca
     2341 atatacctgc aaatgtgaat aagctcgaag agaatattcc aaaaaaggaa gttctcagta
     2401 atggagcacg gcagggtcga aatgattttg gccgaattgg ctatggcgga ccatgcccac
     2461 ctggagggac acaccgttac tattttaagc tctacgcact cgacagagag ctcgacctta
     2521 aagcaggctc cacaaaacgt gacctcctca aagcaatgga aggacacgtt cttgcagaag
     2581 cccatcttat gggcagatat agaaggagtt aatcagtaat cagtaatcag taatcagtaa
     2641 tcagtaagca gtaagcagta agcagtaagc agtaagcaaa aaacaaaggc tccagtactg
     2701 tattattacc ccataaattc cggaaaacat tacagggcat acatgatcct ttagctgaat
     2761 tatcttacat tcaattgcat actttccctc tcccttttaa cttccagcac ctgagtggtt
     2821 ccacctgtct cgagattaat gccatgaggt ttatttgttt tccatgagcc accggtgaaa
     2881 tcaggaacat ctataggtct ggatctgttg gcaaccgacc attcgctcag tggagcaaca
     2941 cagctccatg taacaccgtc ataaacatct tggtcgagcg gcagcccatt cctgaggcag
     3001 tcgataagcc gccagtccat aatgaaatcc atcccaccgt gtccgccaac ccttcttgcc
     3061 atctctccta ccctctttat tatttctggt gtgtatttat cccatatctc ctgcattgct
     3121 tcatctttta cccattcctc tccaaaagca atcatctggc gcggatattt cacagccatc
     3181 cctttcgtcc cttttacaag atgtattctt gaatatgggt gtgacgaaga aacatcgtgc
     3241 tgaactttaa tggtctttcc atgggctgtc cttatcagtg tggtattaaa atttcccctg
     3301 aactttgcat tcctccacgg ttcataaaaa ggatcctgtt caacaagttc tttttcccgg
     3361 agatacattg tgaaatcatc tgtcgacatt gatgtcagat actccatcct gtcgcctctg
     3421 ttgatattca tgacctgtgc tatcggtcca agtccatgag ttggataaag g
//
LOCUS       DAOSNB010000278         3410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_27845_length_3410_cov_3.909091, whole genome shotgun sequence.
ACCESSION   DAOSNB010000278 DAOSNB010000000
VERSION     DAOSNB010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27845_length_3410_cov_3.909091"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="PLG42_09845"
     CDS             complement(<1..1759)
                     /locus_tag="PLG42_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOU31551.1"
                     /translation="MKNSSPFLYVFLFISTITFSQQTGFLSNIYDYLENPAVFEINQT
                     EGHSLVIPYASSTDALAGNLQRSTGYMSLNGTWKFWYSDVPENFPAGFYESTFNDKGW
                     KTITVPSNWEMQGYGDPLFRNVTTPFRPNPPKVPREYNPTGIYRRAFSIPPSWKDKEI
                     FLRMEKTASASFVWINGKEVGYNEGAQEPAEYNITKYVRPGNNTICVLVFKYSDGYYL
                     EDQDYWRLAGIFDNVSVIARQKIHLFDWYGVTDLDGSYRDATLEIEAVIYNYSGSTAD
                     GLTLRAVLYNSNLKEVARFVKENITVAAQGSIKTILTGEVKQPRLWSAEDPYLYHLSF
                     EIIDRNGRVIEASAGRIGFKETEIRNQVFYLNGKPVKLNGINSHMQHPDNGHTMDEET
                     IRKDFTILKQFNINCVRTSHYPPVQRYLELADEYGLYIVDETGDEAHATEYLSSRKEW
                     EAMYRERARKMVLRDRNHPSILFWSAGNESGEGDNICAVIEEGKKYDRTRYWMYGGNA
                     FAHRCEDIIGPRYPNILTLLTRVYNVPPSEDPRPSFLDEYLAVTGNGGGGFDEYWELF
                     NKYSRSMGGAVWDFVSTGIT"
     gene            complement(1927..3288)
                     /locus_tag="PLG42_09850"
     CDS             complement(1927..3288)
                     /locus_tag="PLG42_09850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOU31552.1"
                     /translation="MKKLKLTRRQALTAVSAGAVGSFLKWSFPSFTIVRSEQDKPALL
                     GGAKVRTAPWPSWPIWDREAENSIIEMLRTGRWWRGNGEHVVEFEKKYAELMGAKRCL
                     ATASGTTALMVSLQALGVDAGDEVLVSPFTFIATYNVVFYQKALPVFVDTDPETFLID
                     PSKIESRITERTTAIIPVHIYGLPVDMDRVNEVARKHNLKVIEDACQAWLAEYKGKKV
                     GNFGHTGCFSFQNSKNLPAGEGGAIISDNDELMDLCHSIHNCGRPYGSVKRTSNYPIN
                     GGNNRMQQIQALILMSQMKRILRDFEIRLENAKYLDSKLKEIPGIKTYKLVHESAKSA
                     YHLYPFRFISQEFNNMPRETFIRALNAEGIPCMPGYGPQNKDGLIEQMLTSRGFKRLF
                     PESRLKQWREENVLPGNDQLCREAVIFSQSMLLGTRNDMDDIVNAITKIYKNRQALAE
                     YKG"
BASE COUNT          846 a          754 c          773 g         1037 t
ORIGIN      
        1 gtgttattcc ggtgctgaca aaatcccata cagcaccgcc catgctgcgg gaatatttat
       61 tgaaaagttc ccagtattca tcgaagcctc cgccaccgtt accggtaact gcaaggtatt
      121 catccagaaa tgacgggcgg ggatcctctg aaggaggcac gttataaacc cgtgtaagca
      181 gagtaagaat attcgggtag cgtggaccta taatatcttc gcacctgtga gcaaaggcat
      241 taccaccata catccagtag cgggtcctgt cgtatttctt tccttcctcg ataacagcgc
      301 agatattatc cccctcaccg ctttcgttgc ccgcactcca gaagagaata gacggatggt
      361 ttctgtcgcg cagaaccatt ttccgggccc tctccctgta cattgcctcc cactctttac
      421 gtgacgaaag gtattctgtg gcatgagcct catcgcctgt ctcatcaaca atgtacaggc
      481 catattcatc agcaagttca agatatcgct gaacaggcgg ataatgtgat gtgcgtacgc
      541 agttgatatt gaattgctta aggatagtaa agtcttttct aattgtctcc tcgtccatgg
      601 tatgcccatt atcgggatgc tgcatgtggc tgttgatgcc gttgagcttt accggtttgc
      661 cgttaaggta aaacacctgg ttacggattt ctgtttcttt gaagccgatg cgtccggctg
      721 aagcctcaat tactctccca ttgcggtcaa taatttcgaa tgaaagatga tagagataag
      781 ggtcttcagc cgaccacagg cgtggttgtt ttacctcacc ggtaaggatt gtctttatgc
      841 tgccctgcgc tgcaacagta atgttttctt ttacaaacct tgcgacctct ttaagattgc
      901 tgttgtaaag tacagcccga agtgttaaac catctgccgt gctgcctgaa taattataaa
      961 tgacggcttc gatttccaaa gttgcatccc tgtagctccc atcaaggtca gtaactccat
     1021 accagtcgaa gagatgaatc ttttgacgtg caataactga gacattatca aatattcctg
     1081 ccagtctcca gtaatcctgg tcttcgagat aatatccatc ggaatatttg aaaaccagca
     1141 cgcatattgt attattaccg ggtctgacgt attttgtgat gttatattca gcaggttcct
     1201 gtgctccttc gttatagcca acctcttttc cgttgatcca cacaaaggat gctgaggcag
     1261 ttttctccat cctcaggaaa atttctttgt ctttccatga cggaggtatt gagaatgccc
     1321 ttctgtagat tcctgtggga ttgtattcgc gtggcacttt aggcgggttt ggcctgaatg
     1381 gtgttgtcac gttacggaaa agagggtcgc cataaccctg catctcccag ttggatggta
     1441 cagtgattgt tttccatccc ttatcgttaa aggttgattc gtaaaagcct gccggaaaat
     1501 tctcaggcac atcggaatac cagaatttcc atgttccatt cagcgacata tagccggttg
     1561 atctctgcag gttaccagca agagcgtcgg tggatgaagc gtaaggaatt accagtgaat
     1621 gtccttcagt ctggtttatt tcaaagacag caggattttc gagatagtca taaatgtttg
     1681 agagaaatcc tgtttgctga gaaaatgtta ttgttgaaat aaacaaaaaa acatagagaa
     1741 atggtgatga atttttcatt ggctgatatt tataaaagac cttttataac cattcattca
     1801 actgcaaatg aaacgagttc agatattcat ctctgcatca aacagaggta aggacaagat
     1861 ataattaaaa aatattctcc tctcatccat gaggaaggga ggagaattaa gcggtgttat
     1921 tttcattcag cccttatatt ctgcaagtgc ctggcggttt ttatatattt ttgttattgc
     1981 gttaacaata tcatccatat cgtttcgtgt tccgagcagc atactttgag agaatatgac
     2041 tgcttcgcgg cagagctggt cgtttcctgg caggacgttt tcctcgcgcc actgtttcag
     2101 gcgtgattcc gggaagagtc ttttgaaacc tcttgatgtc agcatctgtt ctatgagtcc
     2161 gtctttattc tgaggtccat aaccaggcat gcagggtatg ccttcggcat tgagtgccct
     2221 tatgaatgtt tctctaggca tgttattgaa ttcctggctt ataaatctga acgggtaaag
     2281 atggtatgct gattttgctg attcatgtac gagtttgtaa gtttttatac caggtatttc
     2341 tttgagtttt gaatcgaggt atttcgcatt ttcaagtcgt atctcaaagt cgcggaggat
     2401 tcgcttcatc tgcgacatca ggatgagtgc ctgtatttgc tgcatgcggt tgttgccacc
     2461 gttgatcggg tagtttgaag ttctcttcac gcttccgtag ggtctgccgc agttgtgtat
     2521 tgaatggcag aggtccatca gttcgtcgtt atcgcttatt attgcgccac cttcgcctgc
     2581 tggcaggttt ttcgagttct ggaagctgaa gcatccggta tggccaaagt tacccacttt
     2641 cttcccttta tattcggcca gccaggcctg gcaagcatcc tctatcactt tgaggttgtg
     2701 cttgcgggct acttcattca ccctgtccat atctaccggg aggccataga tgtgtaccgg
     2761 tatgatggct gtggttcttt cggtaattct cgactcgatt ttagacgggt cgatgaggaa
     2821 agtctcgggg tcggtatcca caaacaccgg gagggctttc tgatagaaga ccacgttata
     2881 agtagcgatg aaggtgaatg gggaaaccag cacttcatcg cctgcgtcga cgccaagtgc
     2941 ctgaagtgac accataagag ctgttgtgcc ggaagctgtt gcgaggcatc tctttgcgcc
     3001 catcaattca gcatatttct tttcgaattc gacaacatgt tctccgtttc cgcgccacca
     3061 cctccctgtc cttagcattt cgataatact gttttctgcc tcacggtccc atatgggcca
     3121 tgatggccat ggagctgttc tcaccttagc ccctcctaga agtgcgggct tatcctgttc
     3181 acttcttaca atggtgaagg aaggaaaaga ccatttcaga aaagaaccga ctgctccggc
     3241 agagactgcc gttaatgcct gacgtcttgt aagtttcagt tttttcatgg ccgggatatt
     3301 taaattataa tctgtcagaa aagcaacaaa ttatgtttaa agttaaagca ggaaataatt
     3361 ttaaacaagt tataaaattt accggaaaat agaaagggat agttaattat
//
LOCUS       DAOSNB010000279         3409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_27869_length_3409_cov_2.445438, whole genome shotgun sequence.
ACCESSION   DAOSNB010000279 DAOSNB010000000
VERSION     DAOSNB010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27869_length_3409_cov_2.445438"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..618)
                     /locus_tag="PLG42_09855"
     CDS             complement(61..618)
                     /locus_tag="PLG42_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOU31553.1"
                     /translation="MEKTHLCPVWVAYTFLLPVRKLQHNPDEILGPYIKPGMKIMDYG
                     CAMGYFSIPLARMTGPQGTVYCVDIQEKMLEKLEKRAVKYKVDKIIKPLLVDRNYHPE
                     EFSEQLDFILLFNVVHEVPDKKQLFYDLSRMMKKDGKILFSEPKGHVRPERFERSVQI
                     AREAGLSVTGEKPVKNGISAVLIRG"
     gene            944..>3409
                     /locus_tag="PLG42_09860"
     CDS             944..>3409
                     /locus_tag="PLG42_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOU31554.1"
                     /translation="MQPLAYLKKFFLNVIILSQAVYSLAQVSDDRPGQEILKMISSAP
                     DDKGSETGALILLKDVRLTVGEKNLKRLEIHLVGKVYKREAVADYAQIPLRYNSFYEE
                     PVLNFARVIYKDGTIKDVRKDGIQIKNLPEMEGLQFTDTRYLSFALTGLEPGTAFEYK
                     VTITDKMPVIENEWSYTQLFSFYLRMLAPPYKPRTDPCLKSVYTLVLPSSRQFNWKSS
                     LPLPDPEMKSLGKTTEYTWTVTDLPAIQMEYGMPPISRIAPVIYLTSLKDWEQVDRWA
                     AKKFLKKIRVNNEIAFRVQNLVAGKRNDLEKVASIFDYIRKSINYLYTDLERGGYEPH
                     YADEVFRSKYGDCKDQTILLISMLKAANIKAWPALINPFPQDEFPDLPNIDFSHLITY
                     LELDGKPLFLDPTAPYCPFPGLTGPDQDRKVFIINGEGGKLLRTPEAKPENNLANFNM
                     EIMFRKGTAIVNFKIDAKGTINEFLKQTFSNTASVSVDDFFKWISRSVSETAVFDTLI
                     MSDLSDPQVPFSAHLIFHIDSIWNAEQGAVGLGSHTELFLSLLMNIDTKNYPNKRYND
                     IVIKYPQIITGREKFILPARDLMCITIPPKDSVVNKWFNFQQGFSHNPTYFTANFSLL
                     LNGNNVDREDYSSYLQSLRTLRQVSGWNVQFIDPLVYIPALTEQENPIHTIAECDKML
                     AEDPNNVYALMASAMAYYKNNQINEAIKRLKEVLGKEPDNKYAHYFLAQCYTAINNRA
                     VARMNLTSALLTDPEFDAARALKATLYFADKNVKDALDEIEEGLRKNPSSIPCLRTKV
                     FILMLSGKSDEAMNDINLLVAADS"
BASE COUNT         1025 a          692 c          712 g          980 t
ORIGIN      
        1 gaacggaagt ttcctaaact ttaaatccag gttcgattcc tggtggggct acaatccata
       61 tcatcccctg ataagtactg ctgatattcc gttctttacg ggcttttctc cggtaacaga
      121 caaaccggct tcccgcgcaa tctgaaccga cctttcaaaa cgctccggtc tgacatgccc
      181 tttcggttcc gaaaacagaa ttttaccatc ttttttcatc atcctggaaa gatcataaaa
      241 gagctgtttc ttatcaggca cctcatgcac gacattaaaa agcaggatga aatcaagttg
      301 ttccgaaaat tcttccggat gataatttct gtctacaagt aatggtttaa ttattttgtc
      361 aaccttgtat ttcactgctc ttttctcaag cttttccagc attttttcct gaatatccac
      421 acagtagacc gtcccttgcg ggccagtcat tctggcaagt gggatgctga aatagcccat
      481 tgcgcaaccg tagtccatta tcttcatccc gggttttatg tatggaccca gtatttcgtc
      541 agggttgtgc tgtaatttac gcaccggcag gagaaaggta taagcaaccc ataccgggca
      601 gaggtgcgtt ttttccatgg aatcgtaatt aaatccaatc aaaaatatag tcaatagttc
      661 cgcatgcaat tgagatttcg gtagaataaa aatttctgcc ggtgaaacac caatatacgc
      721 atctggataa ataaaatttg ctccaaaaag ctgcatgacc gataaaaacg ttaggattgc
      781 cagaggagag gatgattttg aagggaataa tatttgtatg catttgccta taaacagtaa
      841 tatctgtagc caatgttcat tctccagtaa caaattactt agttgcttcc gtatttcatt
      901 ttgggaatat ttttaaattt atgtgatata attgaagttg aatatgcaac cgttagctta
      961 ccttaaaaag tttttcctga atgtaattat tttatcacag gcagtttata gtctggcgca
     1021 ggtttctgat gacagacccg gacaggaaat tctgaaaatg atttcctcag cccccgatga
     1081 taaaggatct gaaacaggag cactgatttt gcttaaggac gtgcgactca ctgttggtga
     1141 aaaaaatctg aaacggctcg aaatacacct cgtaggtaag gtttataaaa gggaagcggt
     1201 tgctgattat gcgcagatac ctctcaggta caattcattc tatgaggagc cggtactcaa
     1261 ttttgccagg gtgatttata aggatggcac tattaaagat gtgaggaagg atggaattca
     1321 gattaagaac ctgccggaaa tggaagggct tcagtttacg gatacccggt atctttcctt
     1381 tgcattaaca ggtcttgaac ctggtacagc attcgaatac aaggtcacta taactgataa
     1441 aatgccggta attgaaaatg agtggtctta tacacaactt ttttctttct atctgaggat
     1501 gctggcgcca ccttataaac ccagaaccga cccttgcctg aaatcagtat atactctcgt
     1561 gcttccttcc tcaagacagt tcaattggaa atcatccttg ccattgcctg atcctgaaat
     1621 gaagagttta ggtaaaacaa cagaatatac ctggacggta accgatctgc ctgcaattca
     1681 aatggagtac ggaatgccac caatcagcag gattgctccg gtaatttatc tgacttcgct
     1741 gaaagactgg gagcaggttg acaggtgggc tgccaagaag tttcttaaaa aaattagagt
     1801 aaataatgaa atagctttca gagtacagaa tttggttgca gggaaaagaa atgacttgga
     1861 aaaggtcgca tccattttcg attacatcag gaagagcatt aactatctgt ataccgatct
     1921 ggaaagaggc ggttatgaac ctcattacgc agatgaagtg ttccgttcaa aatacggaga
     1981 ttgcaaagac cagactattt tgctcatatc gatgctgaaa gcggcgaata ttaaagcatg
     2041 gccggctttg atcaacccgt ttccccagga tgagtttccg gatctgccta atattgattt
     2101 ttcccatttg atcacctatc tcgaacttga cggaaaacct ctttttctgg atccaacagc
     2161 accgtattgt ccctttccgg gattgactgg tccggaccag gacagaaagg tatttatcat
     2221 caatggtgag ggagggaaat tattaagaac ccctgaagca aaacctgaaa ataaccttgc
     2281 caatttcaat atggaaataa tgttccggaa gggaactgcc attgttaatt tcaagattga
     2341 tgccaaagga acgatcaatg aattcctaaa gcagacattc agcaatactg cgagtgtttc
     2401 cgtggatgat ttctttaaat ggatatcccg ttcagtttct gaaaccgctg tatttgatac
     2461 cttgattatg tctgaccttt cggatcctca ggtgccgttc agcgcccatt tgatatttca
     2521 tatcgacagc atctggaatg cagaacaggg tgcggtagga ttaggaagtc atacagaatt
     2581 gtttctgtca ttgttaatga atattgatac gaagaattat ccgaacaaga gatataatga
     2641 catcgttatc aaatatcctc agatcattac cggtcgagaa aaatttattc tgcccgccag
     2701 ggatctcatg tgcataacaa tacccccaaa ggactccgtt gtaaacaaat ggtttaattt
     2761 ccagcaggga ttttctcata acccaactta ttttacagcc aacttcagtc tgctattaaa
     2821 cgggaacaat gttgacaggg aggattacag ttcttatttg cagtcgctga gaacattacg
     2881 tcaggtttcc ggctggaacg tacagtttat tgatcctttg gtatatattc ctgcgcttac
     2941 cgagcaggaa aatccaattc acacaattgc ggaatgcgat aagatgctgg ctgaggatcc
     3001 gaataatgtt tatgctttga tggcaagtgc catggcctat tataagaaca atcagattaa
     3061 tgaagcaata aaaagactta aggaagttct cgggaaggag cctgataata aatacgcgca
     3121 ttatttcctg gcccaatgct atacagccat aaacaaccgg gctgtggcaa ggatgaacct
     3181 caccagtgcg cttcttaccg atccagaatt tgatgcggca cgagctttga aagcaaccct
     3241 ttattttgcc gacaaaaacg ttaaagatgc tcttgatgaa attgaagaag gtttaagaaa
     3301 gaacccatcc agcattccct gtctgagaac caaagtattt attttaatgt tatcagggaa
     3361 aagcgacgaa gccatgaacg atattaacct gctggttgct gccgattct
//
LOCUS       DAOSNB010000280         3384 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_28129_length_3384_cov_4.766597, whole genome shotgun sequence.
ACCESSION   DAOSNB010000280 DAOSNB010000000
VERSION     DAOSNB010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28129_length_3384_cov_4.766597"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..689)
                     /locus_tag="PLG42_09865"
     CDS             complement(303..689)
                     /locus_tag="PLG42_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOU31555.1"
                     /translation="MKTFKKNFVIHAEPSDVYAAITNPYTIELWSGYPAIMKEEPGTE
                     FSLWEGDIEGRNIEFVKNKKIVQEWYFGDQPEQSIVTITIEPVKGNSLVKLEHTNIPD
                     EAFENIKQGWEEFYFGAIQNFFNPNF"
     gene            complement(747..1751)
                     /gene="trxB"
                     /locus_tag="PLG42_09870"
     CDS             complement(747..1751)
                     /gene="trxB"
                     /locus_tag="PLG42_09870"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOU31556.1"
                     /translation="MEIFKPLEVKGSRQEPETEQVEKVRCLILGSGPAGYTAAIYAAR
                     ANLKPVLYTGLEMGGQLTTTTDVENFPGFPEGITGPELMENMKKQAERFGTDIRIGIA
                     TSADFSQRPLKVVIDDRKTILADAVIIATGATAKYLGIEAEKKYAGRGVSACATCDGF
                     FYRGKDVAVVGGGDTACEEATYLAGLCRKVYMIVRRNVLRASKAMQQKVLNTPNIEIL
                     WEHQVKDLFGEEGVEGAILVKKKGTPEEEIVKIKIDGIFLAIGHTPNSGPFSKYIETD
                     ATGYIKTVPGTSKTNIPGVFAAGDVQDPQYRQAVTAAGSGCMAAIDAEHYLAGLESSI
                     "
     assembly_gap    2242..2341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2352..2425)
                     /locus_tag="PLG42_09875"
     tRNA            complement(2352..2425)
                     /locus_tag="PLG42_09875"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2389..2391),aa:Pro,seq:ggg)
     gene            complement(2500..3075)
                     /locus_tag="PLG42_09880"
     CDS             complement(2500..3075)
                     /locus_tag="PLG42_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31557.1"
                     /translation="MRQYLFPAFTVGTVKTKVGKPKNIILNYNTVTEKMVFEQKGQYY
                     DLIGQESVDTIYLQKKKFIPHENFFLEVAFEDKIALGIQHRSNLQAPGKPVGYGGTSE
                     LASANYITGIELSTGYFNLKIPEGYVVRYSPFYWVKKDNEWYKFLSAKQFVNIFPEHS
                     ADLNQFIKKNKIKFDRPDDLIRLVKYCNDTY"
BASE COUNT          923 a          711 c          592 g         1058 t
ORIGIN      
        1 agaaaaatat ctttacctgt cttcattgcc actttaccta cagtaaaatc gggaaacagg
       61 tattgctccg gatattttat gaccacctcc tcaccttcct gtgctttcaa aggaagagag
      121 aataagacaa tcaataagat aagacttgtt attattaatt ttttcattga aaatattttt
      181 ttcatttctt tttgcatctt aaaatctgat tcaaagttaa taaatatatg acaattaatc
      241 atttaaaaag tttaatgaag attagttaaa aatgattaaa ttccaggtga gcggtattct
      301 gttcagaagt taggattaaa gaaattctga attgctccga aatagaattc ttcccatccc
      361 tgttttatat tctcaaaagc ttcgtcgggt atgtttgtat gttcgagctt taccagcgag
      421 tttcccttta ccggttcgat tgttattgta acaattgatt gttcaggctg gtcgccaaag
      481 taccattcct gaactatttt tttatttttt acaaattcaa tgtttctgcc ttcgatatca
      541 ccttcccaca gtgaaaattc agttcctggt tcttccttca taattgccgg ataacccgac
      601 cacaattcaa tagtataggg atttgttatt gctgcataca catcggaggg ctcagcatga
      661 atgacgaaat ttttcttgaa tgttttcatc tggctaagta ttgttccgtt cgtataaaaa
      721 atcttaataa gttttgtttt tatatttcag attgagctct ccagtccagc aagataatgt
      781 tctgcatcta ttgctgccat acagccggag cctgcagcgg tgactgcctg gcggtactga
      841 ggatcctgaa catcccctgc agcaaaaacc ccaggaatat ttgtttttga tgtgccagga
      901 acagttttaa tgtagcctgt agcatctgtt tcaatgtatt tgctgaatgg tccagagttt
      961 ggcgtatgac caattgcaag aaaaataccg tcaattttga ttttcactat ttcttcttcc
     1021 ggtgttcctt ttttcttcac aaggatggct ccttcaacac cctcttcccc gaaaaggtct
     1081 tttacctgat gttcccagag tatttcaata ttaggcgtat taagcacttt ttgctgcatt
     1141 gcctttgaag ccctcagcac atttcttctc actatcatat acacttttct gcaaagacca
     1201 gccaggtatg tcgcttcttc gcaggccgtg tctccacctc ctacaactgc aacatcttta
     1261 ccacgataaa agaatccgtc gcatgtagca caggctgaaa cgcctcgtcc ggcatacttt
     1321 ttctcagctt caatgccaag atattttgca gtagctcctg ttgcaataat aactgcatcc
     1381 gccagaatgg tctttcgatc atcaattaca accttaaggg gccgctgtga aaaatctgct
     1441 gatgttgcaa ttccaattcg tatatcggtg ccgaatcttt cggcctgttt cttcatattt
     1501 tccataagtt caggacctgt aataccctca ggaaatcctg gaaaattttc gacatcggtt
     1561 gttgttgtca actgcccacc catctccagt cctgtataga gcaccggttt caggtttgcc
     1621 ctggcagcat atatagctgc agtataacct gcaggtcctg aaccaagaat aagacatctt
     1681 actttttcaa cctgctcggt ttcaggctcc tgcctgcttc cttttacctc aagcggcttg
     1741 aaaatttcca tatcatttaa tttttgcctg tgcaaagata agtaatggag cgaataataa
     1801 tgtgtgattt aagttacata ttaacaggtt ttgaagtaaa gcccccctct cccatttccc
     1861 tgtccggttg acgccagtca gccgaccaag aaaaagtttt cacatgaacc actgaaatac
     1921 aatgaatttc gaaatcccgc cttgcgggat aaatttcacg gagatcccga aagcataacg
     1981 taccataacc actgaattac aattgaatta caattgaatt acaattgaat tacaattgaa
     2041 ttactactga atgatatata agcccccctg tcaggggggt tggggggtct acaatactat
     2101 gcccctgtca ggggggttgg ggggtcaatt ttttgtcggg gtggccagac ttcccgataa
     2161 atcgggataa actccctgcc gacccctttt ttgtcggggt ggccagactt cccgataaat
     2221 cgggataaac tccctgccga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nccctttttt gtcggggtgg ccagactcga actggcgacc ccttgcaccc catgcaagtg
     2401 cgctagccaa ctgcgccaca ccccgaatag cagtttgcaa aattaatcaa caatttctat
     2461 aatgcaaacc tcctgatacg attcttcaga aatacaatgt cagtaagtat cgttacagta
     2521 tttcacaagc cggattaaat catccggcct gtcgaatttt attttgttct tcttgatgaa
     2581 ctggttcagg tcggctgaat gttcgggaaa gatattaaca aactgcttgg ctgagagaaa
     2641 cttataccat tcgttatctt tttttaccca gtaaaaagga gaatatctta ccacataacc
     2701 ttcgggtatc ttcaaattaa aataacctgt cgaaagctca attcccgtaa tataatttgc
     2761 tgatgcaagt tcagaggtac ccccatagcc tacaggcttt cctggtgcct gaaggttact
     2821 tctgtgctgg ataccaagag caattttatc ttcaaatgca acttcaagaa agaaattttc
     2881 gtggggaata aattttttct tctgcaggta aatagtatcg accgactcct gacctattaa
     2941 atcataatac tggcctttct gttcaaaaac catcttttcg gttaccgtgt tgtaattaag
     3001 aataatgttt ttaggtttgc caacttttgt tttgactgtg cccacagtga aagccggaaa
     3061 gagatactgt ctcatctccg gtggattatt ctcctgagaa gaaactgcag caggaaaaat
     3121 caacccagat atcaaaataa acattactga ccggctgacc tttgaaaaaa atatttccat
     3181 ttctcacatc ggatttaatg taaagttata aatacaagtg ttaacaataa aattatcggt
     3241 taaattttta attctctaat atttcatgtt gtaaatgatt gacttttaca tatttatttc
     3301 gtaattttga taagatgttt aactaaaata aaaacctatg aagctcatgg acattcttgc
     3361 cctcatttcg ggaggcatag gagt
//
LOCUS       DAOSNB010000281         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_28362_length_3362_cov_3.748715, whole genome shotgun sequence.
ACCESSION   DAOSNB010000281 DAOSNB010000000
VERSION     DAOSNB010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28362_length_3362_cov_3.748715"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="lpxB"
                     /locus_tag="PLG42_09885"
     CDS             <1..929
                     /gene="lpxB"
                     /locus_tag="PLG42_09885"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOU31558.1"
                     /translation="RIILRNILLCKKHIIEYQPDVVILIDYPGFNLKIAEFAKSSGFR
                     VFYYISPKLWAWNESRVKKIRRFTDRMFVILPFEVEFYAKHGIEVKYFGNPLVDEIEK
                     WKEKQDELRRDIRKEMELDDRQVIAILPGSREHEIKYILPEMLKVIDHFPEYNFVIAG
                     VSTVSRQIYKEIIGNVPVKILYDSTYNLLSVSVAALVKSGTSTLEAALFDVPQVVCYK
                     GDAISFAIARHLVKVRHISLVNLIMGREVVRELIQFLLTEKNLVESLGEILPGGKERE
                     RMLADYRELKKMLGPAGASERTASEMVACLLK"
     gene            937..2127
                     /locus_tag="PLG42_09890"
     CDS             937..2127
                     /locus_tag="PLG42_09890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOU31559.1"
                     /translation="MRIKKEILSFMLATALILFCPFDLFSEDTIPSKIRIRLFSETET
                     DLLFFSVETGNYNMILFPGYSREIKTGETVVMMEYNGRLAIKISGSRALLADSVFFTS
                     ASGRDLFTLNLNNGSKQNYIYSGDLHCIPDMETILLINICDIEDYVAGVVKAEGGTGK
                     SEEYFKTQAVITRTYTYRNFKRHITDRYNLCDGTHCQVYRGISADSIINSAMHHTAGR
                     VIVDTDSILIISAFHSNCGGETSPSEYVWVISVPYLTSVKDPYCTSSPNAIWEKKVSV
                     REWKNILTANGYTDGTENSSQFTFSQNSRTMHYTTGSFRIPFYVLRNALGLRSAWFSV
                     YDAGDSLILKGRGYGHGVGLCQEGAMVMANKGINYEDIIKFYYPDTRIIDIRSAKMPV
                     EIKK"
     gene            2175..2918
                     /gene="gldF"
                     /locus_tag="PLG42_09895"
     CDS             2175..2918
                     /gene="gldF"
                     /locus_tag="PLG42_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOU31560.1"
                     /translation="MFAIFRKEISGFFSTLTGYLVIIVFMLVNSLFMWILPGEWNILE
                     SGYASLDTLFQLSPWIFLFLVPAITMKMISEEKRLGTIELLYSRPVTEREIVYGKFFA
                     SVALVFLALLPCVIFYVTIYIAGETPGNLDRGGTFGSFLGLFFLASIYASAGIFASSL
                     TSNQVVAFVVSVFICFFLFAGFDSFAYLPGLKKVDEFVSLLGVNEHYRSMSRGVIDLR
                     DLVYFLAGAVIFNEASRLSLLSRKWKRRS"
     gene            2900..>3362
                     /locus_tag="PLG42_09900"
     CDS             2900..>3362
                     /locus_tag="PLG42_09900"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOU31561.1"
                     /translation="MEEKKLKNIFRNDVRLRSWADFILISLAAVIIAAASGFLRVRID
                     LTEDRRYSLSEHTRKILGGLKEDIYIQVYLEGEMPVLFKKLRRSVRELLDEFRIASHR
                     RIDYEFINPSAGSDPAAREKFQEDLIAKGISPVNVMMKDREGGSSQRLIYPG"
BASE COUNT          967 a          626 c          773 g          996 t
ORIGIN      
        1 tccgtataat cctgcgaaac attttacttt gtaaaaaaca tatcatcgaa taccaacccg
       61 atgttgttat cctgatagat taccccggct tcaatttgaa aattgcagaa tttgcaaaat
      121 catcaggctt cagggtgttc tattacattt cgccgaaact atgggcatgg aacgagtcaa
      181 gggtaaagaa gatcaggaga tttaccgatc gcatgttcgt gattttgcca tttgaagtgg
      241 aattctatgc aaaacacgga attgaagtaa aatacttcgg caaccctctt gttgacgaaa
      301 ttgaaaaatg gaaagaaaag caggatgaac tgagaaggga tataaggaaa gagatggagc
      361 tggatgatcg tcaggtgatt gctatcctgc caggctcaag ggaacatgaa ataaaatata
      421 tcctgcccga aatgcttaag gtaattgacc atttcccgga atataatttc gtaattgcag
      481 gggtttcaac tgttagccgg cagatttata aagagattat tggaaatgta cctgtaaaga
      541 ttctgtatga cagcacctac aaccttcttt cagtttcggt tgcagcgctt gttaaatcag
      601 gaacatcaac actcgaggca gctctgtttg atgttccaca ggttgtatgc tataaaggcg
      661 atgctatatc atttgcaata gcaaggcatc tggttaaggt caggcatatt tcccttgtaa
      721 acctgataat gggcagggag gtggtcagag aacttatcca gttcctgctt actgaaaaga
      781 accttgttga atcacttgga gaaatattgc cgggtgggaa agaacgggag aggatgcttg
      841 ccgattacag ggaactgaaa aaaatgctcg gtccggcagg agcttcggag cgtacggctt
      901 ccgaaatggt tgcctgtcta ttaaagtagt cctgacatga gaataaaaaa ggaaatttta
      961 agttttatgc tggccacagc attgatactt ttttgtcctt ttgacctttt ttcggaagat
     1021 accattcctt caaagataag gattcgtctt ttttctgaaa cagaaacaga ccttctgttc
     1081 ttctcggtag aaaccggcaa ttacaatatg atactattcc cgggttattc ccgtgagata
     1141 aaaaccggtg aaacggttgt gatgatggaa tacaacggaa gattagccat taagatatca
     1201 ggcagcagag ctttactggc tgattcggtc ttttttacgt cagcctcagg gagagacttg
     1261 tttacattaa atcttaacaa cggcagcaag cagaattaca tctattcagg tgatcttcat
     1321 tgtattccgg atatggaaac tattttgctg ataaacatct gcgatataga ggattacgtg
     1381 gcaggagtgg taaaagctga aggaggaacg ggaaagagtg aagagtattt caagacacag
     1441 gcggttataa cacgtaccta tacataccgt aattttaaac ggcatattac tgacagatac
     1501 aatctttgtg acggcactca ttgtcaggtt taccggggca tttctgcaga cagtataatt
     1561 aacagtgcta tgcaccatac agcaggcagg gttatagttg ataccgacag tatacttatt
     1621 atctccgctt ttcattccaa ctgcggaggt gaaacttcac cgtcagaata tgtgtgggtt
     1681 atttcagttc catatctcac cagtgtgaaa gatccttatt gtacctcttc ccctaatgct
     1741 atatgggaaa aaaaggtcag tgtgagggaa tggaaaaaca tcctgactgc taacggatat
     1801 actgatggca cagaaaattc ttcacagttt actttcagtc agaactccag gactatgcac
     1861 tatactacag gttcattcag gattccgttt tatgtactga gaaatgctct gggactcagg
     1921 tcggcatggt tctcggttta tgatgctggc gactctttga ttcttaaagg aagaggctac
     1981 gggcatggtg tgggcttgtg ccaggagggt gcaatggtaa tggccaacaa gggaattaat
     2041 tatgaggata ttataaaatt ttactatccc gatacccgaa taatagatat aagaagcgca
     2101 aagatgccgg tcgaaattaa aaagtaatat tcatttaagg taattgacta tttttgcttt
     2161 tcatttgcaa aatcatgttt gcaatcttca gaaaggaaat aagcggcttt ttcagtactc
     2221 tcacaggcta cctggtgata attgtgttta tgcttgtgaa cagcctcttt atgtggatat
     2281 tacccggtga atggaatatc ctggaaagcg gctatgccag cctcgacaca ctttttcagc
     2341 tttccccgtg gattttcctt tttctggttc ctgccattac aatgaaaatg atatcagaag
     2401 agaagaggct tggaactatt gaacttcttt attccagacc tgttacagaa cgtgaaatag
     2461 tgtacggaaa attctttgct tctgtggctc ttgttttcct tgccctcctt ccatgtgtga
     2521 ttttttatgt aacgatatat attgccggcg aaaccccggg caaccttgac aggggaggaa
     2581 cattcggctc atttctcgga ttattctttc ttgcatcaat atatgcttct gcaggtatct
     2641 ttgcttcgtc acttacttca aaccaggttg tggcattcgt ggtatcagtc tttatttgct
     2701 tcttcctgtt tgctggtttt gattcttttg catatttgcc cggactgaaa aaagttgacg
     2761 aatttgtatc ccttcttggg gttaatgaac attacaggtc gatgagcaga ggtgtgattg
     2821 atttacgcga cctggtttat tttcttgcag gggcagttat attcaatgag gcatcacgtc
     2881 tttcattact ttcaagaaaa tggaagagaa gaagctgaaa aatattttca gaaatgatgt
     2941 gcggcttaga agctgggctg attttattct gatcagcctg gcagctgtta ttattgctgc
     3001 tgcttcgggc tttttacgtg tccgcatcga cctcactgaa gacagacgct attccctttc
     3061 ggaacatact agaaagatac tgggagggct gaaggaagat atatatatcc aggtttacct
     3121 cgagggagag atgcccgttt tgttcaagaa actgagaaga tcagtaaggg aattgctaga
     3181 tgagttcaga atagcttcac acaggcggat cgattacgaa tttatcaatc cttcagcggg
     3241 aagcgatcca gctgcaaggg agaaattcca ggaagatctg attgcaaagg gcatctcacc
     3301 ggtaaacgtt atgatgaaag acagggaggg aggttcgagc cagagactca tttatccggg
     3361 ca
//
LOCUS       DAOSNB010000282         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29186_length_3290_cov_4.321484, whole genome shotgun sequence.
ACCESSION   DAOSNB010000282 DAOSNB010000000
VERSION     DAOSNB010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29186_length_3290_cov_4.321484"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PLG42_09905"
     CDS             complement(<1..1016)
                     /locus_tag="PLG42_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU31562.1"
                     /translation="MRFISAQYVITCSGPVLKRPVICTEDDGTIISIKDTGGDLRESE
                     LTEFYNGIIIPGFVNCHCHLELSYLHNKISPSTGLHGFLGELDRTRYNIAGDEEKMAR
                     NADKAMYDEGVVLCADICNSSLTFPLKKESPIRYTNLIEVFGIDHNKAQQRIEEALLL
                     ADIAEKYGLKWNLTPHSVYSVPVPLFRLIKKYSLSNTLTSIHFLESEGEISFLESHSG
                     PLMDVYRNFLPAGMTPLTVKDPISAVLDEVTASGCLILVHNTFIRRQHIQTLRKRENL
                     FYCICPNSNLFIEGKLPPVELLAEENCNVVIGTDSLSSNYELSILSELKTLSMNFPSL
                     SLET"
     gene            complement(1013..3019)
                     /locus_tag="PLG42_09910"
     CDS             complement(1013..3019)
                     /locus_tag="PLG42_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOU31563.1"
                     /translation="MKFSIYIASRYLLAKKSRNAINVISAVSVAGVTVGTMALIIVLS
                     VFNGLEDLINRIFTVFDPDLRITAAEGKVFTPDSSKLLNLSKVPGIEAYSFTLEENSL
                     LKYGDRQYIASLRGVDDNYSKVTGIDSIIVEGEYRLQSVGGRPEAVVGAGVASYLGIN
                     VNFITPLHVYVPKRTGSVNLNPENAFIEKYLFPSGIYQIEHEFDSKYYFVPLDFAREI
                     LEYETEISSIDIRVNKQVGIGPVQKAVKEIFGDAFDIKNRYEQKEIFYKVMRSERLAI
                     FIILTLILIIASFNIIGSLTMLIIEKEKDIIILRSLGADDLLIKRIFLMEGWLISIIG
                     TVAGLILGFIICWTQQRYGLIKLQSETLIVNAYPVVLKLKDFIAVPATVLTIGFFAAW
                     YPVRYLSKKYLKETIHASNLSLRSIIIFIIAITSVLSVSCSKSEENKIKKSEIIPQKE
                     FVNLLTDLYISDGLLSIPLIKEIYQMKDSTLNYIDIIRSHGYTKEKVDLTFKYYFMNN
                     PKKLEKIYDQVIARLSELQSRLESEVSVTPTFNLWNMRPSYSVPSDGVHNKLWFSIPV
                     KDTGTYYFMFDAQVYPDDQSTNLRTKIFFWKPDSTKQGVINYWSPSYYIRDGEKHSYT
                     IKNTLTDSTFTHINGWLLEHDSQPGRWIKHVKITNIRVFKGGVE"
     gene            complement(3016..>3290)
                     /locus_tag="PLG42_09915"
     CDS             complement(3016..>3290)
                     /locus_tag="PLG42_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31564.1"
                     /translation="STEEITDKLKSAGFSNIETTYTYGKPGHISWLISVKYPAMLLGK
                     SLIFAIMLPFYFVIVLPLALILNTIDLNSVHKTGTGLLIKAIKTEK"
BASE COUNT          978 a          613 c          647 g         1052 t
ORIGIN      
        1 atagtctcta aactgaggga aggaaaattc atgctcaggg ttttaagctc cgataaaata
       61 ctaagttcat agtttgacga aagactgtcg gtgccaataa caacattgca attctcttct
      121 gccagaagtt caactggagg gagcttcccc tctataaaaa gattggagtt ggggcagata
      181 cagtaaaaaa ggttttctct ttttctcaat gtctgaatat gttgtcttct tatgaacgtg
      241 ttgtgaacaa gaataagaca acctgatgct gttacttcat cgagcacagc actgatggga
      301 tcttttactg taagaggcgt catgccggca ggaagaaaat ttctatatac atccataagg
      361 ggacccgaat ggctttcgag aaatgaaatc tcaccttctg attcaagaaa atgtattgaa
      421 gtaagagtgt tgctcagaga atattttttt ataagtctga agagaggaac tggaaccgaa
      481 taaacagaat gtggagtaag attccatttc aaaccgtatt tctctgcaat atcagcaaga
      541 agcagcgcct cttcaattct ttgctgtgct ttattgtggt ctattccaaa tacttctata
      601 agatttgtgt atctgattgg gctttctttt ttcaatggga aagtcaatga agaattacat
      661 atatcggcac acagaaccac accttcatcg tacatggctt tatctgcatt tcgcgccatt
      721 ttttcttcat ctccggcaat attgtacctt gtccggtcaa gttcaccaag gaaaccgtgc
      781 aaacctgttg atggtgaaat tttattatgc agataggata attcgagatg gcagtggcag
      841 ttaacaaaac cgggaattat tattccgttg taaaattcgg ttaattcact ttccctcagg
      901 tctccgcctg tatctttaat acttataatt gttccgtcat cttctgtgca tataacagga
      961 cgtttaagga ctggcccgga acatgttatg acatattgtg ccgatataaa tctcattcga
     1021 ctccaccttt aaagaccctt atgttggtaa ttttcacatg ttttatccac ctacctggct
     1081 gggaatcgtg ctcaaggagc cagccgttta tatgtgtgaa agtagaatca gttagagtgt
     1141 tttttattgt gtatgaatgt ttttcaccgt cgcgtatata gtaggaagga gaccagtaat
     1201 taattacccc ctgctttgtg gaatcgggct tccagaaaaa aatttttgtt ctcagattag
     1261 tactctgatc atcaggataa acctgtgcat cgaacataaa gtagtatgtc cctgtatctt
     1321 tgacaggtat gctgaaccat aatttattgt gaaccccatc ggaaggaact gagtatgagg
     1381 gacgcatatt ccacaggttg aaagtggggg tcacactgac ttcactttca agccttgact
     1441 gaagctcgct cagccgggct attacctgat cgtaaatttt ttccagtttt ttggggttat
     1501 tcatgaaata gtacttaaaa gtaaggtcaa ctttctcctt tgtgtaaccg tgcgaccgga
     1561 ttatatcaat ataattgagt gttgagtctt tcatctgata aatttcctta ataagcggaa
     1621 tcgacaacaa accatcagaa atataaaggt cagtaagaag gtttacaaat tctttctgag
     1681 gaattatttc actctttttg attttgtttt cctcagattt tgagcaggat actgacaata
     1741 ctgaagtaat tgctatgatg aaaataatta tacttctgag tgaaaggtta gatgcgtgta
     1801 tcgtttcttt aaggtatttt tttgaaagat accttaccgg gtaccatgct gcgaagaaac
     1861 caatagtaag cactgtggca ggtacagcaa taaaatcttt cagtttcagc actacaggat
     1921 atgcgttaac gataagtgtt tcactctgaa gctttatcag tccgtacctc tgctgtgtcc
     1981 agcaaattat aaatccgagt attaaaccgg caacggttcc gattattgaa atcagccatc
     2041 cctccataag aaaaattctt ttaataagaa gatcgtcagc tccgaggctc ctgagaataa
     2101 taatatcttt ttctttttca ataataagca ttgtcagtga gccgataata ttaaatgagg
     2161 caatgataag tatgagagtc aggataatga aaatagccag tctttctgat cgcattacct
     2221 tataaaatat ttctttctgc tcatatctgt tttttatgtc aaaagcatct ccgaagatct
     2281 ctttcactgc tttctggaca ggtccgatac caacctgttt atttacccgg atatctatag
     2341 agcttatttc ggtttcgtac tcaagaattt cacgggcaaa atcgagaggt acaaaataat
     2401 attttgagtc aaattcatgt tcaatctggt agatgcccga agggaataaa tatttttcga
     2461 tgaaggcatt ttcggggtta aggttaacac tacctgtgcg tttagggaca tagacatgaa
     2521 gaggggtaat aaaattgaca ttaattccca gatagctggc aactcctgct cccacaacgg
     2581 cttcaggccg accaccaaca ctttgcagcc ggtattcacc ctcaacaatt atactgtcga
     2641 tacctgtaac ttttgagtag ttgtcatcta ctcctcttaa tgatgcaata tactgacggt
     2701 cgccgtattt cagaagtgag ttttcttcaa gggtaaatga ataggcttca atgcccggta
     2761 cttttgaaag atttaggagt tttgaagagt caggtgtaaa cactttgcct tctgctgcgg
     2821 tgattctcaa atcgggatcg aaaactgtaa aaatgcggtt tatcaaatcc tcaaggccgt
     2881 tgaatactga aagaacaatt ataagtgcca ttgttccaac ggttacacct gcaactgaaa
     2941 cagctgatat gacattgatg gcatttctcg atttttttgc cagcaaatat cttgatgcta
     3001 tgtaaataga aaacttcact tttcagtttt tatggccttt ataagtaaac ccgttccggt
     3061 tttatgtact gaattcaggt caattgtatt aagaattaaa gctagaggca gaactatcac
     3121 gaaataaaaa ggtaacataa tggcaaaaat aagggatttg ccaagtaaca tagcagggta
     3181 ctttactgat atcagccacg atatatggcc gggttttccg tatgtataag ttgtttcaat
     3241 attactgaaa cctgccgatt taagtttatc tgtaatttct tctgtgctat
//
LOCUS       DAOSNB010000283         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29267_length_3282_cov_3.933065, whole genome shotgun sequence.
ACCESSION   DAOSNB010000283 DAOSNB010000000
VERSION     DAOSNB010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29267_length_3282_cov_3.933065"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="miaB"
                     /locus_tag="PLG42_09920"
     CDS             complement(<1..1103)
                     /gene="miaB"
                     /locus_tag="PLG42_09920"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOU31565.1"
                     /translation="MNIADSEIVASVLNESGYDVTRDINEASLILLNTCSIRENAEQK
                     IWNRLKALKSVRKKNPSVMIGVIGCMAERLKEKLIQNDLPVDIVAGPDSYRSLPDLVT
                     EAEIGQSRINVMLSESETYADISPLRLDSNGVTAFIAIMRGCNNMCTYCVVPFVRGSE
                     RSRDPRSIVKEATELFRKGYREVTLIGQNVNSYLWKDAGETIGFPELLAEVARIDPLL
                     RVRFATSHPKDLSDKLLETISSYDNICKHIHLPVQSGSNRILKLMNRQYTREWYMNRI
                     EAIRKIIPECSVSTDIIAGFCTETEEDHKDTLSLMEWAGFDFAYMFKYNERPGTKAAE
                     SLPDDVPDEIKTKRLNEIINLQNKISAKSKKADV"
     gene            1290..2036
                     /locus_tag="PLG42_09925"
     CDS             1290..2036
                     /locus_tag="PLG42_09925"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOU31566.1"
                     /translation="MSGHNKWSTIKRKKSAQDAKRGKIFTKVIKEITIAAREGGGDPD
                     LNPRLRLAIQNAKGVNMPKENIERAIKKALGAEGSNYIETTFEGYGPCGVAVFVECLT
                     DNNNRTVSSIRATFNKYGGSLGTNGSLSYLFERKGVFTIKPEGINLEELQLQLIDAGA
                     EDIEEEGGRIIVTCAREDFGAINKKLQELGIEAEEAMLKRIPNDTKKLDVESAKKVLK
                     FIEALEDDDDVQNVYHNLEMTDELAAVLEQ"
     gene            2096..3196
                     /locus_tag="PLG42_09930"
     CDS             2096..3196
                     /locus_tag="PLG42_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31567.1"
                     /translation="MQRTLFLLVLLLLSACSKVHDNFLWYRSFGKGEAFFVSAAGDTG
                     LVSGGIIENKPYLVFLNGKHEKVFEYSVDTSGSFTSAIACEEGIVTAGCIEDKLYLGM
                     MDYTGTPVWSSGFSSSFKVGNVILCRTQKDNFLAVASRASDSIPGGNYGIKFISFGKD
                     GSVISNDSIIYYSFYSVNGATIDNSGNIYLAATKLGAGSKKKATLAKYDEAFRKIWEV
                     ELYNNPQFEASSMSVVTDYQGNVYVSGYTEYPEDATTYRNAFAASVNSSGVIRWKKYL
                     EYKNEATSVIFDNNGQLFVLNKGCLILNIIKTDDGSVSGILRTFAACDVSYDKVYGNS
                     ATAWGGNSIVIAGVKNGSFYIVARSSEILSPI"
BASE COUNT         1013 a          585 c          708 g          976 t
ORIGIN      
        1 acatcggctt ttttactttt tgctgagatc ttattctgaa gattgattat ttcgttaagc
       61 ctttttgttt tgatttcatc aggaacatca tcgggaaggg actctgcagc ttttgttccg
      121 gggcgttcat tgtacttgaa catgtaggca aaatcaaaac ctgcccattc cataagtgat
      181 agagtatctt tatggtcttc ttcggtttcg gtgcagaacc cggcaataat atctgttgat
      241 accgaacact cagggataat ttttcttatg gcttctatcc tgttcatgta ccattccctt
      301 gtatattgcc tgttcattag cttgagaatg cggttgctgc cactctgaac ggggaggtgg
      361 atatgtttgc agatattatc gtaagatgag attgtttcca gaagtttgtc tgacaggtct
      421 ttcggatgtg aagtggcaaa tcttaccctc agcaaagggt caatacgagc tacttctgcc
      481 agtaattcag gaaagccaat agtttctcct gcatctttcc ataaatatga atttacgttc
      541 tgtccaataa gtgttacttc cctgtaacct tttctaaaca gttcggtagc ttctttaact
      601 attgagcgag gatctctgct tcgttcagaa cctcttacaa aaggtaccac gcagtaggtg
      661 cacatattat tacatccccg cataattgca atgaaggctg taacaccatt gctgtcgagc
      721 cttaaggggg atatatcggc ataggtttca gattccgaga gcattacatt gattctgctc
      781 tgaccgattt cggcttctgt aacaaggtcg gggagcgaac gataagaatc gggcccggca
      841 acaatgtcta ctggaagatc gttttgtata agtttttctt taaggcgttc agccatacat
      901 ccaattacgc caatcattac agaagggttt ttctttctta ccgatttaag ggcctttagc
      961 cggttccaga ttttttgctc tgcattttca cgtatagaac aagtattaag taaaatcagg
     1021 ctggcttcat tgatatcccg ggtaacatca taaccagatt cgttcaatac tgatgcaaca
     1081 atttcactgt cagcaatatt catctgacag ccgtaagttt caatgtataa cttttttctt
     1141 atcaaatcct tgaattataa ttaaattttt gtgttgcaaa agtatcaaaa aaaagaatct
     1201 atgtaataag cataaagcag catacttatt gttaatatgg tttgaaagaa ataattaatt
     1261 ttgcacataa ataattaaaa catatttgta tgtcaggtca caataaatgg tcaacaatca
     1321 agagaaagaa gagtgctcag gatgcaaaga ggggcaagat attcacgaag gttataaagg
     1381 agataacaat tgcagcaagg gaaggaggag gtgaccctga tctcaatccc aggctgagac
     1441 tggcaataca gaatgccaag ggtgtaaata tgcccaaaga gaatattgag agggcgatca
     1501 aaaaggcact tggagccgag ggttcaaatt acatcgaaac cacttttgaa ggttacgggc
     1561 catgcggtgt agctgtgttt gtggaatgcc tcaccgataa caacaatcgc actgtttcgt
     1621 caatcagggc aacatttaac aaatatggcg gcagtctcgg cacaaacggt tcgctttcat
     1681 atctgtttga aagaaaagga gtttttacga tcaagcctga aggaatcaac cttgaagaac
     1741 ttcagttgca actcattgat gccggggcag aggacataga agaggaaggt ggcagaatta
     1801 tagtgacttg tgcaagggag gactttggtg caataaacaa aaaacttcag gagttgggaa
     1861 ttgaagccga ggaggctatg ctgaagcgga taccgaatga cactaaaaaa ctcgatgtgg
     1921 aatcagcaaa aaaagttctg aaattcattg aagcactcga agatgatgat gatgtccaga
     1981 atgtttatca caacctggaa atgaccgatg aacttgctgc ggtacttgaa caatgattta
     2041 atacggtaat gtaatattgt aaaaattgcg ggaatcataa aaaccccgat atataatgca
     2101 aagaactctt tttttactgg ttttgctcct tttatcagcc tgcagcaaag tccatgataa
     2161 tttcctctgg tacagatcct tcggaaaagg tgaagctttt tttgttagtg cagccggcga
     2221 caccggattg gtaagtggcg gaattattga aaataaacct tacctggttt tcctgaatgg
     2281 aaaacatgaa aaagtattcg aatatagtgt cgacacatcc ggttctttta catctgcaat
     2341 tgcctgtgaa gagggtatag taacagcagg ctgcattgaa gacaagcttt atttagggat
     2401 gatggattat acaggaactc ccgtgtggag ttcaggtttc agttcatcat ttaaagtagg
     2461 gaatgtaata ctctgcagaa cacaaaagga taactttcta gcggttgcct caagagcttc
     2521 tgactcaatt cctggtggaa attacggaat caaatttatt tccttcggaa aagatggctc
     2581 tgtaatatcc aatgacagca ttatttacta ttccttttat tcagtaaatg gagcaaccat
     2641 cgataattca ggaaatattt accttgctgc aacaaaactg ggagcaggaa gcaaaaaaaa
     2701 ggctacactg gcaaagtacg acgaagcttt tagaaaaata tgggaggtgg aactttataa
     2761 taaccctcag ttcgaagcat catccatgtc ggttgtaacc gattatcagg ggaatgttta
     2821 tgtgtcaggg tatactgaat atccggaaga tgcaaccact tacagaaatg cttttgcagc
     2881 atctgttaat tcctcaggcg ttatcaggtg gaagaaatat ctcgaatata aaaatgaggc
     2941 aacttcagtg atatttgaca ataatggaca actatttgta ctaaacaaag gttgccttat
     3001 acttaatatt attaaaacag acgatggttc agtaagtggt attctcagaa catttgcagc
     3061 atgtgatgtt tcatatgata aagtctatgg gaacagtgca acggcatggg gtggaaattc
     3121 aattgtaatt gccggtgtaa aaaatggttc attttatatt gtagcaagat cctctgagat
     3181 tctatctccc atctaacaca gcgttgatga gcaaaaagat gaaaaacatt tttaaagtag
     3241 ggcagcttgg tagagtgttg tttttcctgg tatttatttt tg
//
LOCUS       DAOSNB010000284         3276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29334_length_3276_cov_4.535238, whole genome shotgun sequence.
ACCESSION   DAOSNB010000284 DAOSNB010000000
VERSION     DAOSNB010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29334_length_3276_cov_4.535238"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..658)
                     /locus_tag="PLG42_09935"
     CDS             complement(164..658)
                     /locus_tag="PLG42_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOU31568.1"
                     /translation="MKRAIFLDRDGVINRKGGPYYIYLKEDFVLNEGVTEALKYFMHK
                     GFLLIIITNQGGIAKGVYTEEQMHEVHNFMKELLKKDGIEIAGIYYCPHHPDVSPCEC
                     RKPGTLLLEKAIKDFNIDTASSWMIGDSEIDIQAAEKMGIKGVLIPENGNMMELIVKP
                     GLIT"
     gene            complement(659..1675)
                     /gene="holA"
                     /locus_tag="PLG42_09940"
     CDS             complement(659..1675)
                     /gene="holA"
                     /locus_tag="PLG42_09940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOU31569.1"
                     /translation="MKAESHEEILQDLKNKIYKPVYFLAGDEPYYIDLISDYIEEKVL
                     DESEKAFNQIIMYGDGVTPDQIIDVARRYPMMAAHLVVIVKEAQLIKKIEDLMVYVQN
                     PQKSTILVIDYKYKTLDKRTKFYKTIAETGVYFESNRLRDYEVPAWIENYLKKKGIKA
                     EPDAGRMLTEYLGTDLHKIINELEKLTITLPEDRPVITLSHIEKNIGISKDFNNFELQ
                     KAIGEKNAYKAALIIKYFESNPKDNPVVLTIASLFSYFSKILAYHCLTDKSKNNVAAA
                     LGIHPFFVREYETAAANYNMRKVVEIISLLRTYDMKTKGFGDAGTPPAELLKELVFKI
                     IHLS"
     gene            complement(1678..2448)
                     /locus_tag="PLG42_09945"
     CDS             complement(1678..2448)
                     /locus_tag="PLG42_09945"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOU31570.1"
                     /translation="MKTKDEIVTNWLPRYTGKPLDSFGKYILLTNFSVYIELFARWHN
                     VKIEGKDRNMPSATANGITIINFGMGSPNAATVMDLLSAIKPHAALFLGKCGGLKKKN
                     KLGDFILPIAAIRSDGTSNDYLPQEVPALPAFKIQIVVSSVIARYNLDYWTGTVYTTN
                     RRVWEYDDRFKKYLVKTRAMAIDMETATIFTVGFANEIPVGALLLVSDQPMIAAGIKT
                     EESDKLVTEKYVETHLRIGIDSMKEIKEKKLSVKHLRF"
     gene            2570..3037
                     /locus_tag="PLG42_09950"
     CDS             2570..3037
                     /locus_tag="PLG42_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOU31571.1"
                     /translation="MYLKELNLPPYSFKITGVPGEEKIFDDIRRRYVRLTPEEWVRQN
                     FIKYLIGEGGYPAGLIGVEVTFTMNRLRKRADILVHNREGRPVMIVECKSYDVVLDES
                     VFDQIVTYNMRFRVPYMVVTNGMINYACKIDFENNSWEYLMVIPQYEELIKEQ"
     gene            3034..>3276
                     /locus_tag="PLG42_09955"
     CDS             3034..>3276
                     /locus_tag="PLG42_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOU31572.1"
                     /translation="MTDVTCAIIRDGDAKVLVVQRGEESDHPFKWEFPGGKTLQGESH
                     EDCIIREIREELALDIVICSLLEPVEYDYGFKQIRLI"
BASE COUNT          895 a          630 c          719 g         1032 t
ORIGIN      
        1 tgaaagaaaa ttcatgggta atagcaagga taagtccgga agttattatg accagccgtg
       61 gacccgatac atggaagaaa gtactgaaaa gtcttaataa gaagtacagg atctgggccg
      121 actttcctga gtcgcccgat atgaactgag attcttcagg gaatcaggta atcaaccctg
      181 gttttacaat cagctccatc atatttccgt tttcagggat aagaactcct tttattccca
      241 ttttttctgc agcttgaata tcaatttcac tgtcgcctat catccacgat gatgcagtgt
      301 caatattaaa atcttttatt gctttttcga ggagcagggt gccgggtttg cgacattcgc
      361 agggcgaaac atcgggatga tgaggacaat aatatattcc tgctatctca atgccatcct
      421 ttttcagtag ctctttcatg aaattatgca cctcgtgcat ttgttcctct gtataaacac
      481 ctttagcaat acctccctga ttggttataa taatcagcag aaaacctttg tgcatgaaat
      541 atttaagtgc ttcagtaact ccttcattga gcacaaaatc ttccttgagg taaatgtaat
      601 agggtccgcc tttacggtta atcactccgt cacggtcaag aaatatcgcc cttttcattc
      661 aggaaagatg aattattttg aataccagtt ctttaagcag ttctgcaggc ggcgtaccgg
      721 catcaccgaa accttttgtc ttcatgtcat aggttctcag caggcttatt atctcaacaa
      781 ctttacgcat attgtaattg gcagcagccg tttcatattc gcgtacgaag aaaggatgta
      841 tccctaatgc tgctgcgaca ttatttttcg atttatcggt taggcaatgg taagcgagga
      901 ttttggaaaa atagctgaag agtgatgcga tggtcaggac taccgggtta tcttttggat
      961 tgctttcaaa atactttatg atcaatgccg ctttgtaggc atttttctcg ccaattgctt
     1021 tctgtagttc gaagttgttg aaatccttgc ttatgcctat gtttttttca atatgactga
     1081 gagtgataac aggtctgtct tcaggcaggg taattgtaag tttttcaagt tcatttataa
     1141 ttttatgaag atcggtgcca aggtattcgg taagcatcct tcctgcatca ggctcagcct
     1201 taataccttt tttcttaaga taattttcta tccatgccgg aacttcgtaa tccctcagcc
     1261 tgttcgattc aaaatataca cctgtttctg caatggtttt gtaaaacttt gtccgtttgt
     1321 cgagagtttt gtacttgtaa tcaattacaa ggatagttga tttctgaggg ttctggacgt
     1381 agaccatcag gtcttctatc tttttgatca gctgggcttc ctttacaatc acaaccagat
     1441 gagcagccat catcggataa cggcgggcaa catcaattat ctgatcgggg gtgacgccat
     1501 caccatacat aatgatctga ttgaaagcct tttcactttc gtcgagtact ttttcttcaa
     1561 tataatctga aatcaaatct atataatagg gctcgtcacc tgccaggaaa tagacaggtt
     1621 tgtaaatttt atttttcaga tcctgtaata tctcttcgtg actttcagct ttcataatca
     1681 gaatcttaag tgtttcaccg acagcttttt ttcttttatt tccttcatgg agtcaatgcc
     1741 tatcctcagg tgggtctcaa catacttttc ggttacaagt ttatctgatt cttcggtttt
     1801 aataccggct gctatcatag gctggtccga caccagcaga agggcaccaa caggtatttc
     1861 atttgcaaaa cctactgtaa aaatagttgc cgtttccatg tcaatagcca tagccctggt
     1921 ttttacgaga tattttttga atctgtcgtc atattcccac accctccggt tggtggtgta
     1981 gacggtgcct gtccagtaat caaggttata gcgggcaata acagatgata ccactatctg
     2041 tattttgaat gccggcaggg ctggaacttc ctgcggcagg taatcgtttg aagtaccgtc
     2101 gcttctgatt gcagcaatag gcagaatgaa atcgccgagt ttgttctttt tcttcagtcc
     2161 gccgcatttc ccaagaaaca acgcagcgtg aggttttata gcgctcagga gatccattac
     2221 tgttgcagca ttcggacttc ccataccaaa attgattatt gttatgccgt tggcggtagc
     2281 actcggcatg tttctgtctt taccttcaat tttaacattg tgccatctgg caaacagttc
     2341 aatgtaaaca ctgaaattgg tgagaagaat atatttcccg aaactatcaa ggggtttgcc
     2401 tgtgtagcgg gggagccagt tggttactat ttcatctttg gttttcatgc ctgagttttg
     2461 tgttgttaaa agtcaatata tttgttaaaa taaccgctca gagagggtag tttttaatta
     2521 cgggataaat gtagtgaaaa catcgtggtt gtaaaaataa taaggcaaag tgtatctgaa
     2581 agaactcaat ctgccgccgt acagtttcaa aataaccgga gtgcccggtg aggagaaaat
     2641 atttgatgat atcagaagga gatatgtaag acttacgcct gaagaatggg taaggcagaa
     2701 cttcataaag taccttatcg gggaaggtgg ctatcctgca ggccttattg gggtggaggt
     2761 gacttttaca atgaacaggc tgaggaagag agccgacatc cttgttcata accgtgaagg
     2821 caggcctgtg atgatagttg agtgcaaatc gtacgatgtg gtactcgatg aaagtgtttt
     2881 tgaccagatt gtgacctaca acatgcggtt cagggttccc tatatggttg tgaccaacgg
     2941 tatgataaac tatgcatgta aaattgattt tgagaataat tcatgggaat atctgatggt
     3001 tattcctcaa tatgaagaac tgattaagga acaatgacag atgtaacgtg tgccataata
     3061 agggatgggg atgcaaaagt acttgttgtg cagaggggag aggagagtga ccatccattc
     3121 aagtgggagt ttccaggcgg gaagacactg cagggagagt cgcacgaaga ttgcatcatc
     3181 agagagataa gggaagaact ggcactggat attgttattt gctcactgct tgagccagtt
     3241 gaatatgatt atggtttcaa acagataagg cttatt
//
LOCUS       DAOSNB010000285         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29409_length_3269_cov_3.965775, whole genome shotgun sequence.
ACCESSION   DAOSNB010000285 DAOSNB010000000
VERSION     DAOSNB010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29409_length_3269_cov_3.965775"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLG42_09960"
     CDS             <1..184
                     /locus_tag="PLG42_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31573.1"
                     /translation="LVASPDHPATSPRIATTTSEAFATDRASPSSFRAFASVSSFDAV
                     CEGVPKKVSAAFSPGP"
     gene            complement(341..1060)
                     /locus_tag="PLG42_09965"
     CDS             complement(341..1060)
                     /locus_tag="PLG42_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31574.1"
                     /translation="MKTFKTLFFSGLLLLQAAIIPAQTADQIIEKYLAAIGGRDMLKK
                     VNSVYIESTVDAMGMQMPQKITILNGVGYKQEIDVMGSLLVNCFGASSGWTINPFAGM
                     TSAENMPENQYKEGKDQIYIGAPFSVIKEKGYKLELAGNEDVKGISAAKVKLTSSDGV
                     TTFHYFDPQTGFLIKSLQQGEMEGQPVEITVMYEDYKTVEGLMIPFRTYTSIGSMFEM
                     NNTVTKVEINKPVDPSVFIKP"
     gene            complement(1074..>3269)
                     /locus_tag="PLG42_09970"
     CDS             complement(1074..>3269)
                     /locus_tag="PLG42_09970"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31575.1"
                     /translation="INITDKMKEDGTLEWIPPEGKWRILRIGYSLTGQQNSPASPEAT
                     GLEVDKLNPDHVRNYFTRYLDMYKDATGGLMGDRGLKYIITDSWEAGVANWTDRMIEE
                     FNRRNNYDIIPWLPVLTGKIVENASKSEKFLWDFRNTLEMLVAEHHYDNLTSILKERN
                     MGRYTESHESGRAFIGDGMEVKRSADIPMSATWTPGGLGGSESGGVAVRHETDIRESA
                     SVTHIYGQNIVAAESFTTGRNAWAFDPERLKPTADFMLAAGLNRFVIHTSVHQPVNDK
                     IPGLGLGPFGQWFTRHETWAEQAKPWLDYLSRSSFMLQQGKFVADIIYIYGQGCNLTA
                     LFGNSLPPVPAGYNYDFLNAGAVPSVLSVKDGKIVTPSGMEYKLLVLDESTKYMTLPV
                     LEKIKELVISGAVVAGPKPVDTPSLNDDEAEFQNIVSELWTEEKGVKTSGKGKIYSGY
                     TIAEVLGDMKVEPDFINVADSSGSVMKFVHRKLGNDHIYWVSTSDKTNSVKKVSFRLT
                     GKKPEIWNPVSGEIYPASYKVENGRTEVTLRMSPEDALFIVFRGRTGKKSLELAEPVE
                     TTLATVEGEWKVKFQSGRGAPAEAVFSTLTPWNENEDPGIKYFSGTAEYSINFKAEEQ
                     WLKDDAELWLDLGEVENLAEVLLNGKSLGIVWKKPFRVKLGDALKTGENELKVKVTNL
                     WVNRLIGDQQPGVTKPITYTTQAFYRADSRLLPSGLLGPVNIIKISSNQ"
BASE COUNT          774 a          789 c          682 g         1024 t
ORIGIN      
        1 gctggttgcc tcacccgacc atcctgctac ctcgccgaga atagccacaa ccacatccga
       61 ggcctttgca acagacagag cctcaccgag cagcttccgt gcatttgctt cagtttcttc
      121 tttcgacgct gtctgtgaag gggtgccaaa gaaagtgagt gccgcattca gtcccggacc
      181 ctgatagccg ctgcggcaat cagtgatccg aggtatggga tttcccccag gtgggggaag
      241 gagcttatat tgtgcattca gtctctctcc tctccatctg gggaggccgg gagggcattg
      301 tcaatattcc gaaaatttcc ggcactcccc ttaaacgctt tcagggtttt ataaaaactg
      361 atggatcaac aggtttattt atctccacct ttgtaactgt attgttcatt tcaaacatgg
      421 agcctatgct tgtataggtt ctgaagggaa tcattagtcc ttcaacagtt ttgtaatctt
      481 catacattac tgtaatttcc accggttggc cttccatttc gccctgctga aggctcttaa
      541 taagaaaacc agtctgtggg tcaaaatagt ggaaagtggt aacgccgtca gagcttgtaa
      601 gttttacttt ggcagcactg attcctttaa cgtcctcatt acctgcaagt tcgagtttgt
      661 atcctttttc ctttatcaca gaaaaaggtg cgccgatata aatctgatcc ttaccctctt
      721 tgtactggtt ttctggcata ttttcggccg atgtcatacc agcaaatgga ttaatggtcc
      781 agccactgct ggcaccgaag caattgacaa gcaaggatcc catgacgtca atctcctgtt
      841 tatacccgac accattcaat atcgtaatct tttgaggcat ctgcataccc atagcatcaa
      901 ctgtactttc gatataaacc gaatttactt ttttcaacat atctctgcct ccaattgcag
      961 caagatattt ttcgatgatc tgatcggcgg tttgtgctgg aataattgct gcctgaagca
     1021 aaagaagtcc tgagaaaaac aatgtcttga aagttttcat aataatttat tttttattga
     1081 tttgaagaaa ttttaattat gttcactggt ccaagcaggc cggaaggcag aagtctggag
     1141 tcggccctgt agaaagcctg tgttgtgtat gttattggtt ttgtgacgcc tggctgctgg
     1201 tcgccgataa ggcggttaac ccacaggttt gtcactttta ccttaagttc attttctccg
     1261 gttttaagtg cgtcgcccag ttttaccctg aagggctttt tccagacaat gcccagggat
     1321 ttgccgttca acagtacttc tgcgaggttt tccacttcac ccaggtcgag ccatagttct
     1381 gcatcatctt taagccattg ttcttcagcc ttaaagttta tcgaatactc tgcggttcct
     1441 gagaagtact taatacctgg atcctcattc tcattccatg gtgtcagggt actgaacaca
     1501 gcttctgccg gggctcccct gccagactgg aattttacct tccactcccc ttcaacagtt
     1561 gcaagggtgg tctcaacagg ttcagccagc tcaagggatt ttttcccggt cctgcccctg
     1621 aacactataa acagtgcgtc ttcaggtgac atacgcaggg tgacttctgt gcgaccattt
     1681 tccactttat atgatgcagg atatatttct cctgataccg gattccatat ttcaggtttt
     1741 ttgcctgtta agcggaagga aactttttta accgagttag ttttatcgga tgtgctaacc
     1801 cagtatatat gatcgtttcc aagtttgcgg tgaacaaatt tcattacact gccggagcta
     1861 tcagcaacat ttatgaaatc tggttccact ttcatatcac caagtacttc ggctattgta
     1921 tagccactgt aaattttccc ttttcctgag gttttgactc ctttttcttc tgtccataat
     1981 tcactgacaa tattctggaa ttcagcctca tcatcgttga gtgaaggagt gtcgacaggt
     2041 tttggtccgg caacaacggc accgctgatt acaagttcct ttatcttttc aagaaccggc
     2101 agggtcatat attttgtgct ctcatcgaga acgagcagtt tgtattccat tcccgaaggg
     2161 gtaactattt ttccgtcttt tacagaaaga actgaaggca cggctcccgc attcagaaaa
     2221 tcgtaattat atcctgcagg tacgggtggc aggctgtttc caaagagggc agtaaggttg
     2281 cagccctgac cgtatatata gatgatatca gcaacaaatt taccctgctg gagcatgaat
     2341 gagcttctcg acaggtaatc gagccacggc tttgcctgtt ctgcccatgt ttcgtggcgg
     2401 gtaaaccatt gtccgaatgg ccccagtccg aggccgggta tcttatcatt gaccggttga
     2461 tgtactgaag tatgtatcac aaacctgttt agtccggctg ccagcataaa atcagccgta
     2521 ggctttagtc tctcaggatc aaatgcccat gcgtttcttc ctgtagtaaa cgattcggct
     2581 gctacaatgt tctgtccata aatatgggtt accgatgccg attcccttat atccgtctca
     2641 tggcgtacag caaccccacc tgattcgctc cctccaaggc ctccgggggt ccatgtggca
     2701 ctcatgggaa tatcagcgct tcttttcact tccatgccat cgccgataaa ggcacgaccg
     2761 ctctcgtggg attcggtata ccgccccata tttctttctt taagtattga agttagatta
     2821 tcgtagtgat gttctgctac aagcatttca agcgtgttac ggaaatccca cagaaacttt
     2881 tcgctctttg aagcattttc aactatcttt cctgttaata ccggcagcca cggaatgata
     2941 tcataattgt ttctcctgtt gaattcttca atcattctgt ccgtccagtt tgccacacct
     3001 gcctcccagc tgtcagtaat aatgtattta agtcctctat ctcccatcaa tccacctgta
     3061 gcatctttat acatatcgag gtaccgggtg aaataatttc ttacatgatc aggattaagt
     3121 ttatcaactt caagtcctgt tgcttcggga gaggcgggac tattttgctg gccggtaagt
     3181 gaatagccga ttctgagtat tctccattta ccttccggtg gtatccattc gagggttcca
     3241 tcttctttca ttttatcagt aatgtttat
//
LOCUS       DAOSNB010000286         3257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29555_length_3257_cov_3.920050, whole genome shotgun sequence.
ACCESSION   DAOSNB010000286 DAOSNB010000000
VERSION     DAOSNB010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29555_length_3257_cov_3.920050"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLG42_09975"
     CDS             complement(<1..1100)
                     /locus_tag="PLG42_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HOU31576.1"
                     /translation="MRTLISWLAYNNDFHRGGEKGPLKGVNKEGPNYSMHKYFWSYDR
                     HILLYSGIGDEAGAEMLKNEIIREFPEHKIEIVNANIKDPINLAEIKPVVEARLLDIE
                     GDEIDIFASPGTPTMQVAWYICHTTLKLKTRILQMRPPDKAKTGKRELIEMKIEQSPT
                     PVTAVIKEKRLAERGMKENYLITNSLKPVYDLAEKIAQNDRTTVLIYGETGTGKEHLA
                     RYIHNNSPRKDREFITVNCSAFGDQLLESRLFGYKKGAFTGAEKDTKGLFQMADRGTI
                     FLDEIGDISPYMQQALLRVIQEKEVHPLGGKSEKVDVRIIAATNRNLPEMCKEGKFRW
                     DLYYRLSVVELSLPPLAERPHEDLEMMIDFFL"
     gene            1295..1729
                     /locus_tag="PLG42_09980"
     CDS             1295..1729
                     /locus_tag="PLG42_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31577.1"
                     /translation="MYDTFYSAKKIILGVMNEIRTELSRTENDLIALKEKYNRKRLLF
                     VFSGNIAEKQQMKFEYECSEESIITRIKTLTWLYDMLKSYSRTNCDMIDIMDIEDLLK
                     DLANTAEKVGMIQTAKLFRYWYRCVIHHRMMLHKPVDNGEFI"
     gene            1820..2953
                     /locus_tag="PLG42_09985"
     CDS             1820..2953
                     /locus_tag="PLG42_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOU31578.1"
                     /translation="MKKSIIYFLLCVMLIPAAIAQQEGYLFTIKKELPCTPVKNQSST
                     STCWCFSGISMLESELLRMGKKPIDLSEMFVVRLTYEKKADMHVRMHGSSNFAGGGEY
                     GDVLTICSEYGLVPDAVYPGLNYGEKVHNHGELDAVLKGYVEKLTERSRLTTAWMNGF
                     KGILDAYLGKVPETFTWEGKTYTPASFIKELGINPGDYVTITSFSHHPFYEKFVLEVP
                     DNWAAGLFYNLPIEEMMQVIDYSINNGYTVAWASDVSDRGFSMKEGVAVVPEKDWNEM
                     TTEEAENVFKAPVKQRVITQEMRQKEFDNFSTTDDHGMHIVGIATDQQGNIYYKVKNS
                     WGETGKYKGFIYVSRPFVMLRTTNIMVNKNGIPPAIAQKLGLK"
BASE COUNT          949 a          670 c          641 g          997 t
ORIGIN      
        1 tccaaaaaga aatcaatcat catttcaaga tcctcatgtg gcctttcggc aagcggagga
       61 agcgacagtt ccaccaccga tagcctgtaa tatagatccc acctgaattt cccttccttg
      121 cacatctctg gcagattcct gttggtggca gcgataattc tgacgtctac tttttcgctt
      181 tttccaccca gcgggtgaac ttctttttcc tgaataaccc tgagcagtgc ctgttgcata
      241 tagggtgaaa tatcacctat ttcatccaga aagattgttc ccctgtcggc catctggaaa
      301 agtcctttcg tgtctttttc agcaccggtg aaagcgcctt ttttataacc gaagagtctc
      361 gattcaagca gctgatcacc gaatgccgaa cagttaaccg ttatgaattc cctatctttt
      421 cttggtgaat tgttgtgtat atagcgtgcc agatgctctt tccctgttcc tgtttcacca
      481 taaatcagga ctgtcgttct gtcgttctgg gcaatttttt ccgcaagatc atatactggt
      541 tttagagaat tggtaatcag gtaattctct ttcatacccc gctccgcaag tcttttttcc
      601 ttaatcactg cagttacagg cgtaggagat tgttcaattt tcatctctat aagctccctt
      661 ttccccgtct ttgccttatc aggcggtcgc atctgtaata ttctggtttt aagtttcagt
      721 gtggtatggc atatatacca cgcaacctgc atcgtgggag ttcctggtga agcaaaaatg
      781 tcaatctcat caccctcaat atctaacagc cgggcttcaa ctaccggctt tatctctgca
      841 agatttattg gatccttaat attggcatta actatctcta ttttgtgttc cggaaattct
      901 ctgataattt catttttcaa catttccgct cccgcttcat ctccgatacc agaataaagc
      961 agaatgtgcc tgtcatagct ccagaaatac ttatgcattg aataattcgg accctccttg
     1021 tttacccctt tcaatgggcc tttctcacct ccccggtgaa aatcattatt gtaggcaagc
     1081 catgaaatta atgttctcat aatatcaaaa tttgatataa aaatataaaa atatgataaa
     1141 tatttttcat aattgttttt agtattatct gttttgagta tcctaatata ttgtatttca
     1201 aattgtttac gttaattatt tttttgtgat aatatttatt aaacagagaa caatctttgc
     1261 caaactggtt catgtcatta aaaattacac ggtcatgtac gatacttttt attcggcaaa
     1321 gaaaataatc ctgggagtta tgaatgaaat ccggactgaa ctgtctcgta cggaaaacga
     1381 tttaattgcg ttaaaagaga aatataaccg gaaacgattg ctttttgtat tctcaggaaa
     1441 tattgctgaa aagcaacaaa tgaaatttga atatgaatgc agcgaggaga gcattattac
     1501 cagaattaaa actcttacat ggctttatga tatgctgaaa tcttattcaa gaactaattg
     1561 tgacatgatt gatattatgg atattgaaga tttattgaaa gatttagcca atactgcaga
     1621 aaaggttgga atgattcaaa cagctaaatt gttccgttac tggtaccgct gtgttattca
     1681 ccatcgtatg atgctgcata aacctgttga taatggagaa tttatttaga tcgaataaaa
     1741 tgaaagaaga attaattgat atcttttgaa tatgattgca aatacttgta tatttttaca
     1801 ggtttcaaat cacaaaaaaa tgaaaaagtc aatcatttat tttcttctgt gtgtaatgtt
     1861 aattcctgcc gccattgcac agcaggaggg ctatctgttt acaatcaaaa aagaactgcc
     1921 atgcactccg gtaaagaacc agtcgtcgac aagcacctgc tggtgttttt cgggtatatc
     1981 gatgctcgaa tctgaactgc ttcgtatggg taagaaaccc atcgatttgt cggagatgtt
     2041 tgttgtgagg ctcacttatg aaaagaaggc tgatatgcat gtgcgtatgc acggcagctc
     2101 aaactttgca ggcggaggtg aatacggcga tgtgctaacc atatgcagcg aatatggact
     2161 tgtgcccgat gcagtctatc ccggattaaa ctatggtgag aaagttcata accatggtga
     2221 gctcgacgcc gtattgaaag gttacgtaga aaaactcacc gaacgctccc ggctgacaac
     2281 cgcatggatg aacggcttca aaggaatact cgatgcctac ctcggtaaag tacccgaaac
     2341 atttacatgg gagggcaaaa catacacccc agcttcattt ataaaagaac tgggaatcaa
     2401 tcccggcgat tatgtaacca ttacatcatt cagccaccat cctttttatg aaaagtttgt
     2461 gctcgaggtg cccgataact gggctgcagg gcttttctat aatcttccga tcgaagagat
     2521 gatgcaggta attgactatt caatcaacaa cggctacact gttgcatggg caagtgatgt
     2581 gagcgaccgc ggtttctcga tgaaagaggg tgtggcagtg gtaccggaga aggactggaa
     2641 cgaaatgacc accgaagagg cagaaaatgt ctttaaagca ccagtgaaac aaagagtgat
     2701 tacccaggag atgagacaga aggagttcga taatttctcc accaccgacg accatggcat
     2761 gcacattgtt ggcatcgcaa ccgatcagca gggcaatata tattataagg taaagaactc
     2821 ctggggcgaa accggcaaat acaaaggttt catatatgta tcgcgcccat tcgttatgct
     2881 gcgtaccacc aacattatgg tgaataaaaa cggcatcccg ccagccatag cccagaaact
     2941 tggattgaag tagtcgtata aatttttgat aaactttaat ttaacttcta tttatacaat
     3001 aaccgcccat tgtgtttcct tttaacataa actgaccccc ttttgtattt ctttttaact
     3061 taaactgacc cccctttgta tttcccccca cggggggaaa ttatgataaa atcctattta
     3121 tcaatccttt taagctttcc cccttggggg aaacgggaaa gggggtcata acttagtatc
     3181 gtcctgaaaa aaattgacag ttttttctct ctacatttga agatgccccc tctttacgaa
     3241 gtagagaggg ggttggg
//
LOCUS       DAOSNB010000287         3248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29662_length_3248_cov_5.651425, whole genome shotgun sequence.
ACCESSION   DAOSNB010000287 DAOSNB010000000
VERSION     DAOSNB010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29662_length_3248_cov_5.651425"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1219
                     /locus_tag="PLG42_09990"
     CDS             101..1219
                     /locus_tag="PLG42_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOU31579.1"
                     /translation="MADSKVFFTNLRASSSVNLLKKFDNLIRKAGIETIDFKRKLTAI
                     KIHFGEPGNLAYLRPNFAARLVKLLKEKDAIPFLTDCNTLYYGRRSNAPWHLEAAFEN
                     GYNPLAVNCPVIIADGIKGTEYREIELNLEYCKSAKIGAAIAESDVIISMSHFKGHEI
                     TGFGGAIKNLGMGCASIGGKLFLHSGSSPEIYEENCTGCRICEKYCNYGAIKVGKDRI
                     AHINKELCTGCGQCVAVCQFDAARVVWQNASEAVNKKTAEYALAAVKNKPAFHINFLV
                     DISPDCDCWDFNDYPIVPDIGIAASFDPVALDQASADMVNAAPVLPGSRIYGKAEKQK
                     DDRQDRFKMTHPNTFWQAALEHAEKIGLGRRKYEIIEV"
     gene            1269..2117
                     /locus_tag="PLG42_09995"
     CDS             1269..2117
                     /locus_tag="PLG42_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOU31580.1"
                     /translation="MNGKVKVARLSVMSNTLLIVLKLVAGLISGSVSIISEAIHSSMD
                     LIAAVIAFFAVRVSDNPPDKRHPYGHGKVENISGVIEAILIFIAAVWIVYEAIKRMIA
                     GSPELDMVWLGLVIMVISGIINTFVSWRLYKTARRTGSVALEADALHLKTDVYTSLGV
                     AAGLGLIIITNIKWIDSVVAILVALIILFESYRLLKKAFTPLLDESWPEEDLITLKKT
                     LDEMGVNYHELRTRAAGNYLFIDLHVEVPKYESVENSHKYCDMIEEKLNEQFNNLNVT
                     IHVEPV"
     gene            2151..2603
                     /locus_tag="PLG42_10000"
     CDS             2151..2603
                     /locus_tag="PLG42_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOU31581.1"
                     /translation="MSLAEKVSNDLMAAMKSKDNVVLDALRAAKTAFTLARAEKGVDS
                     VLTPDEETKVIQKLVKQRLESAEIYKQNNRPELYEKEMAEARVLEKYLPAKMSDEELT
                     SVLKTIIERVGAKTPADLGKVMGVAMKELAGKADGKEISAKVRTLLGG"
     gene            complement(2621..>3248)
                     /locus_tag="PLG42_10005"
     CDS             complement(2621..>3248)
                     /locus_tag="PLG42_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOU31582.1"
                     /translation="PIHRDYILNAKFMASLIVVSVMFFALGFLVMGFGLISIGIPPTP
                     GEFLRIIIFIFISIIYVAFWLNLSIVFSVKFRQPATAALSGIAIWLFFAIFYEMIVDL
                     IMKGLVPPTGLVTEAQMINFENFRLNLLRISPSQLFSDATTTLLMPNVRSLGPLSLVQ
                     VIGTIPGPLSLTQSLMLVWPQVTGLIAATIICFVISYVSFMRKEIRSR"
BASE COUNT         1060 a          666 c          687 g          835 t
ORIGIN      
        1 agtccttgtg cccttcagca aggcaattaa actgcacaaa gatgtcaaca gagacctact
       61 gactcttgta agaatacttt ccatctaatt ttaattaact atggcagatt caaaagtatt
      121 tttcacaaat ctcagagcca gttcatcagt taaccttctt aaaaaatttg ataatcttat
      181 ccgcaaagcc ggaattgaga caattgactt taaaagaaaa ctcacggcca taaagattca
      241 ttttggcgaa cccgggaacc ttgcctacct gcgaccaaat tttgcagccc ggctggttaa
      301 attgcttaag gaaaaagatg ctattccatt cttaaccgac tgcaatacat tatattacgg
      361 aagaagatcc aatgctccct ggcatctcga agcagcattt gaaaacggat acaaccccct
      421 ggcagtaaat tgccctgtaa taattgccga cggaatcaaa ggaacagaat accgcgaaat
      481 agaacttaac cttgaatact gtaagagtgc aaaaatagga gcagccatag ccgaatcaga
      541 tgttattatc tccatgagtc acttcaaagg tcacgaaata actggattcg gaggtgcaat
      601 taaaaacctc ggcatgggat gtgcttccat tggaggcaag ctttttctcc attccggctc
      661 atctcccgaa atatatgagg aaaactgtac agggtgcagg atatgcgaaa agtactgtaa
      721 ctatggagca attaaagttg ggaaagacag aatagcacat attaataaag aattatgtac
      781 cggttgcggc cagtgtgttg ctgtatgcca gttcgatgca gcaagggtag tttggcagaa
      841 tgcatcagaa gctgtcaaca aaaaaacagc tgaatatgcg cttgcagcag taaaaaataa
      901 accagccttc catatcaact tccttgttga tatttcgccc gactgtgact gctgggattt
      961 caacgactat cctattgtac ccgatattgg tattgctgct tcgttcgacc ctgtggcact
     1021 cgaccaggca tctgccgata tggtaaatgc tgcacctgtt ctgccaggaa gtagaatata
     1081 cggcaaggcg gaaaaacaaa aagatgacag acaggaccgt ttcaaaatga cacaccccaa
     1141 taccttctgg caggctgctc tcgaacatgc tgaaaaaata ggactgggga gacgtaaata
     1201 tgaaataata gaagtataac tatatactta gttctacgtt aattttttct ataaatcaac
     1261 cttattagat gaacggtaaa gtaaaggtcg caagattatc agtaatgtca aatactctcc
     1321 ttatcgttct caaacttgta gcaggtctga tcagcggttc ggtgagtatc atttcagaag
     1381 ctatccactc atccatggat ctgatagctg cagtaattgc attctttgct gtgagagtct
     1441 ccgacaatcc gcccgacaaa agacatccat acgggcatgg aaaagttgag aatatttcag
     1501 gggtaataga agctatactg attttcatcg ctgctgtatg gattgtttat gaagccataa
     1561 aacggatgat agccggttca ccagaacttg atatggtctg gctcggattg gtcattatgg
     1621 tcatttctgg cattattaac acttttgtgt cgtggagact atacaaaact gcccgaagaa
     1681 ccggctcagt ggctcttgag gcagatgcac ttcaccttaa aacagatgtc tacacctctc
     1741 ttggagtggc agccggtttg ggacttatta tcatcaccaa cataaaatgg atcgattcag
     1801 tagttgccat tctggttgca ttgattattc tttttgaatc ttaccggctt cttaagaaag
     1861 cctttactcc cctgctggat gagtcatggc ctgaagaaga tttaataact ctcaaaaaaa
     1921 cacttgatga gatgggagta aattatcatg aactccggac tcgtgcagca ggcaactacc
     1981 ttttcatcga ccttcatgtt gaagtaccca aatatgaatc ggtggaaaac tcccacaaat
     2041 attgtgacat gattgaagaa aaactgaacg aacagtttaa taatcttaat gttacaatac
     2101 acgttgagcc ggtataattt aattttacat aaattttaaa agttaaagct atgtcactgg
     2161 cagaaaaagt ttcaaacgat ctgatggcag caatgaaatc gaaagacaat gttgttctcg
     2221 acgcgctgag ggcagctaaa acggccttta cacttgcccg tgccgagaaa ggagttgatt
     2281 cagtgctgac gcccgatgaa gagactaaag tgattcagaa actggtaaaa cagcgtctgg
     2341 agtcagctga aatctacaaa cagaacaatc gccctgaatt atacgaaaag gagatggctg
     2401 aagccagggt tctggaaaaa tatcttcccg caaagatgag tgatgaagaa ttaacctcag
     2461 ttcttaagac aataattgag cgagttggcg caaaaacacc tgccgacctt ggcaaggtta
     2521 tgggtgttgc catgaaagaa cttgcaggta aagctgatgg caaagaaatt tccgcaaaag
     2581 tcagaaccct actgggaggt taaagtaata atgataatta ttatcttgaa cgtatctcct
     2641 ttctcataaa agagacgtaa gatatcacaa aacatattat agtggctgca atcagccctg
     2701 taacctgagg ccacaccagc atcagactct gagtcagcga taatggtccg ggtatggtgc
     2761 caataacctg tacaagagat agaggtccca ggcttcttac attgggcatc agcagagttg
     2821 tagtagcatc actgaaaagc tgacttggcg aaattctcag aagattcagc ctgaaattct
     2881 caaagtttat catctgtgct tctgtcacca ggccggtagg cggaaccagc cccttcatta
     2941 taaggtcaac gatcatttca taaaatatcg caaagaaaag ccatatagca atgcccgaca
     3001 gtgctgcggt ggctggttgg cggaacttga ccgaaaaaac tatcgagaga ttcagccaga
     3061 aagcaacata aataatgctt ataaaaatga agataattat tctgagaaac tcaccgggcg
     3121 taggaggaat acctatagaa ataagcccaa aacccataac aagaaaaccc agtgcaaaaa
     3181 acataacact cacaactata agcgatgcca tgaatttggc gttaagaata tagtcgcggt
     3241 gaatgggc
//
LOCUS       DAOSNB010000288         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_29971_length_3220_cov_5.979147, whole genome shotgun sequence.
ACCESSION   DAOSNB010000288 DAOSNB010000000
VERSION     DAOSNB010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29971_length_3220_cov_5.979147"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..510
                     /locus_tag="PLG42_10010"
     CDS             103..510
                     /locus_tag="PLG42_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HOU31583.1"
                     /translation="MKRNRVIDAEFFYGATPEIFFRAAELRRNMTDAEKLVWERLRNK
                     KISGLTFRRQHPVNQFIADFYCHKARLVIEIDGSIHEIDGYKEKDMGRDDEFSKFGLA
                     TIRFTNREIFEKLDTVISRIETLCKSRIKDLSL"
     gene            926..1981
                     /locus_tag="PLG42_10015"
     CDS             926..1981
                     /locus_tag="PLG42_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOU31584.1"
                     /translation="MNIKTFLITLLTVSFLFSCKSKKPEDLEELARRSSRPEAVLVKT
                     VKLEPSTYYHELISNGKANSSRKAVVPFKVNGIIKELNIKNGQKVNTGDLLAVLEDFE
                     YKSRLTSAQQALEKAEINFRDDLLSSYYVTDTTGLSPAKIKISRIRSGLNDAETALDL
                     ARYNFNNTRLYAPINGTVANLEAMQWNPSQDYKNLCTIIYDEIMNVTFPVIESEFAFL
                     RKGMPVNIIPFINDSTLITGTITQINPQVDETGMISVKAGFRNNGRLIDGMNVRVVIR
                     KPVPDRLVVPKEALVIRQGKDVIFVRQDSLAIWKYVTVEFENSKSISVKEGLEPGDLV
                     IISGNVNLAHETTVREE"
     gene            1978..>3220
                     /locus_tag="PLG42_10020"
     CDS             1978..>3220
                     /locus_tag="PLG42_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOU31585.1"
                     /translation="MKKEAHYSSFLSTFTVNIIFVMLIIVGAAMIPLLSLQLNPTRYL
                     PSITISWSWPEAPVRVVEQEVTTTLEGILTTVTGVKKISSSTFNEGGNITVEFDKNVD
                     LRAKRFEVASLLRESGKRLPERVSYPVISMNMPSNQSGSTILSFQLNGNASSAYIYNL
                     AEEVIKPAIALVEGVYSVNVYGATPQEWEVIYDQAKLAAIGASSSAISNAINNYLLER
                     ELGGASEIVTGGPEKRTYLTLEGNSSETFSWDRIPVAKAAGRIIHLTDVAQIKLQDQR
                     PQSYYRINGLNTVNINVSAGRNVNNIKVAEGVKNVMERIKNELPAGYSIRTSSDNTVY
                     LKEEISKNIFRSVLSVILLLLFVLAISREFKYLLIITISLVANILIAFIFYYLFKLEI
                     HLYSLAGITVSFGIIINNTIVM"
BASE COUNT         1028 a          670 c          713 g          809 t
ORIGIN      
        1 tagcaacgat atagataaat ttaaccaatg ttgtaaattt ttgtcatgta cttagaaaaa
       61 gtactaactt tatttagtaa attattccct taaatataaa atatgaaaag aaacagggta
      121 atagatgcag aattctttta tggtgctact cctgaaatat tctttagagc tgccgaatta
      181 agaagaaata tgacagatgc tgaaaaatta gtatgggaaa gacttaggaa caaaaagatc
      241 tcgggattga cattcagaag acaacatcct gttaatcaat ttatagcaga tttctactgt
      301 cataaagcca gattagttat tgagattgac ggttcaattc atgaaattga cggttataaa
      361 gagaaagaca tgggaagaga tgatgaattc agcaaattcg gactagctac gatccgtttt
      421 actaatagag aaatatttga aaaattagat accgtcatca gcagaattga aacattatgc
      481 aaatcaagga ttaaagattt atcgttgtga tagaaatcaa atttggaggt tctgatacta
      541 ctgttatctg cccatcccgt tacccgcccc tcccggcctc cccaggaggg gagggggaag
      601 aaaagaacat tgcttaaaag acccctctgt tatctgcccc tcccggcctc cccaaatggg
      661 gaggaggaag aaagcatcgc ggctaaagtc ctccatctgg gggatttagg gagtgactta
      721 ggagggaagg agtagacaaa tatccgttct tcaaagtccc ccttttgggg gatttagggg
      781 gcagttaata atgggcagtt taactgtggg atttaggggg cagagttctt ttctaacatt
      841 ttttcctaaa aacatttctt catgttaata ttattgctta gatttatagt taaataatgc
      901 aaaacggatc gtaatcttca taaaaatgaa tatcaagacc tttctgatta ctttactgac
      961 agtatcattt ctcttctcct gcaaatctaa aaaacctgag gatctggagg agctggcccg
     1021 ccgctcatca cgacctgaag ccgtgcttgt gaaaactgtt aaactggaac catcaaccta
     1081 ttaccacgaa ctgataagca acgggaaagc taattcctca agaaaagctg tcgtgccctt
     1141 taaagtgaac gggataataa aagagctgaa cataaaaaac ggacagaagg tgaatacagg
     1201 cgaccttctt gccgtgcttg aagatttcga atacaaatcg agactgacat ctgcacaaca
     1261 ggctcttgaa aaagccgaga taaatttcag ggacgatctg ctgagcagct attatgtcac
     1321 cgatacaacc ggactgtcgc cggcaaaaat aaagatatcg cgcataagaa gcggtctcaa
     1381 tgatgccgaa acagctcttg accttgccag gtataatttt aataacaccc ggctgtatgc
     1441 acctataaac gggacagtag ccaacctgga agcaatgcag tggaacccgt cacaggatta
     1501 taagaacctg tgcacaatca tttatgacga aataatgaat gtcactttcc cggtgataga
     1561 atcggaattt gcttttctca gaaaaggtat gcctgtaaat attataccat ttatcaacga
     1621 cagtacatta ataaccggaa caatcaccca gataaatccg caggtggacg agacgggcat
     1681 gataagcgta aaagccggat tccgcaacaa cggccggctt atcgacggca tgaatgtcag
     1741 ggtggttatc cgcaaaccag tgccagacag actggtagtg cctaaagaag cccttgtaat
     1801 acgtcagggg aaagatgtga tcttcgtacg gcaggactcg cttgcaatct ggaaatacgt
     1861 cactgtcgag tttgagaaca gcaaaagtat ctccgttaag gaaggactgg agcccggtga
     1921 ccttgtaata ataagcggaa atgttaatct tgcacacgaa acaacagtga gggaggaatg
     1981 aaaaaggaag ctcattacag ttctttcctt tccacattca ctgtcaacat aatctttgtt
     2041 atgctgatca ttgtcggtgc ggcaatgatc cctttgctct cacttcagct aaacccgaca
     2101 cgctatctcc cttcgatcac aataagctgg agctggcctg aagccccggt acgggttgtc
     2161 gaacaggaag ttacgacaac gctcgaaggc atccttacaa cagttaccgg cgtgaagaag
     2221 atatcatcct caacatttaa cgagggtggt aatataacag ttgagttcga taagaatgtc
     2281 gatctgaggg ccaaaagatt cgaagttgcc tctctgctca gggagtcagg caagagactg
     2341 cctgaaagag tatcgtaccc ggtcatctca atgaatatgc cctcgaacca gtcgggttcg
     2401 acgatactga gcttccagct caacggcaat gccagctcag catatatata taaccttgcg
     2461 gaagaggtca ttaaacctgc tatagcgctt gtcgaaggtg tctacagcgt taatgtatat
     2521 ggcgcaacgc cccaggaatg ggaagtgata tacgatcagg caaagcttgc cgcaataggt
     2581 gcaagttcat cagctatcag caacgcgata aataactatc ttctcgaaag agagctcggc
     2641 ggagcttcgg aaatagtaac aggcggacct gaaaaaagga cttacctgac acttgagggg
     2701 aacagctcgg aaacattcag ctgggaccgg atccctgtgg caaaggcagc aggtcgcata
     2761 atacacctca ccgatgttgc acagataaag cttcaggacc agcgccccca aagctactac
     2821 aggatcaacg gactaaatac agtcaatatt aacgtcagcg ccgggaggaa tgtcaataac
     2881 ataaaagtgg cggaaggggt gaagaacgtc atggagagaa taaaaaatga gcttccggca
     2941 ggttatagca taaggacatc gtctgataat accgtgtatc tgaaggagga aatatcaaag
     3001 aatattttca gatcggtgct gtcagtcata ctgcttcttc tgttcgtcct tgcaataagc
     3061 cgcgagttca aatatcttct cataattact atcagccttg tagccaatat actcatcgca
     3121 tttatcttct actatctttt taagctggag atccatctct actcacttgc cggtataacc
     3181 gtctctttcg gtatcataat aaacaatacg attgtcatga
//
LOCUS       DAOSNB010000289         3201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_30168_length_3201_cov_3.326764, whole genome shotgun sequence.
ACCESSION   DAOSNB010000289 DAOSNB010000000
VERSION     DAOSNB010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30168_length_3201_cov_3.326764"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLG42_10025"
     CDS             complement(<1..273)
                     /locus_tag="PLG42_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOU31586.1"
                     /translation="MEGKDCDRDIIDLLQKDTSKGFRLLVERYSRKLYWHIRRLVILH
                     EDADDALQNTFMNAWKNLSSFRHESSLYTWLYAIATNEALAIFNRRN"
     gene            403..1500
                     /gene="ychF"
                     /locus_tag="PLG42_10030"
     CDS             403..1500
                     /gene="ychF"
                     /locus_tag="PLG42_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOU31587.1"
                     /translation="MALQCGIIGITNVGKTTIFNCLSGTRVETSSAAFSASKSNIGVI
                     HVPDPRLYELRKFQVTEKIVPTTVTIVDIPGLVRGSSRGEGVGNRFLADIRNTDALIH
                     VLRCFDDELLPHVDGSVDPVRDIETVNLELQVKDLESVEKKIERLSKIAKSGDKDAKR
                     GIEILSHLKEHFENFGNARDLNLTGEEKKYIDDLFLLTIKPVLYVCNVDEKSVHTGNK
                     YVEQVKEYLKGKDAEVLVIAGKMEAEIAELENDEDRMAFLNDAGLDEPGVNKLVRAAY
                     RLLNLITFFTVGPKEIRAWTIKKGTNAQQAAGVIHSDLERGFIRAEVMKYNDFVTLGS
                     EHACKEAGKFHVEGKNYIVEDGDILHIRFNV"
     gene            1506..>3201
                     /locus_tag="PLG42_10035"
     CDS             1506..>3201
                     /locus_tag="PLG42_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOU31588.1"
                     /translation="MNTRIIILSLLIFLLSAVSFSQVTRDEKLAEEVKKYGEAEVVIS
                     YPGRNAIELITRNVSITAVKDKKVYISLTAAALNWFLSQRFDYEIIEKEPAKGIISAS
                     SVSGAMEWDSYPTYTQYDSIMRKFQADYPQLCQLDTIGTSILGRLVLVLKISDNVNDD
                     EPEPEVFYSSTIHGDELGGFVLMLRLADYLLKNYNTDSRVRNLVDNLEIWINPLANPD
                     GTYRTGNEIISPTRSNANGIDLNRNFPDPMNPSIVPEKENIDMMAFMRKRRFVLSANF
                     HSGFEVVNFPWDRWLSKYHADHDWFYYISRKYADTVHVYSGPAYMNYYDNGVVRGAEW
                     YIIYGGRQDFITWELQGREVTIELDDIKQTPAAQLELLWQYNRQSLIGYLENALYGIH
                     GRVVDAVTKKPVPAKIYIAGHDKDSSHVYADSLTGYFTRFLAQGSWNLTFSATGYKDT
                     VVQGVNVVNNQRTDLYVEMKSTPTDIDSVNPEKPLLFPNPSSEIIECRLPDSITGQVK
                     VSVINSNGVKILEYLTSDRPVILNVASLDAGVYTVVFKSTVTGHSASSRFVVYSRHY"
BASE COUNT          968 a          591 c          738 g          904 t
ORIGIN      
        1 atttcttctg ttgaatattg caagtgcctc attagtggcg atggcatata gccatgtgta
       61 caatgaactt tcgtgcctga agctgctcag gtttttccat gcattcatga aggtgttctg
      121 gagggcatcg tcggcatctt cgtgaagaat aaccagacgg cggatatgcc agtataactt
      181 ccggctgtag cgttcaacca gaagcctgaa gcctttactg gtatcctttt gaagaaggtc
      241 tataatatcc ctgtcacagt ctttgccttc caaatccaac ctctctttta attattagat
      301 tgtaattttc aaaaaagttt aaaaagatgt aatccaaatt tcagtaaaaa aataatctca
      361 aagaaggaaa ttgtaccttt gtatgatctt ttttaaacgc ttatggcact gcaatgcgga
      421 attataggga ttacaaatgt aggtaagaca acgattttca attgtctctc aggcacaaga
      481 gttgaaacca gtagtgcagc tttcagtgcg tcgaaatcta acataggtgt tattcatgtt
      541 cccgatcccc ggctttatga gctcaggaag tttcaggtaa cggaaaagat agttcccaca
      601 actgtgacta tagttgatat accggggctt gtacgtggct caagcagggg agaaggtgtg
      661 ggaaacaggt tccttgccga tataagaaac acagatgcac ttatacatgt tctcagatgc
      721 ttcgacgatg agttactccc gcacgttgat ggttcggttg atcccgtaag ggatattgaa
      781 actgtaaacc tcgagctgca ggtcaaagac cttgaatcgg tggagaagaa aattgaacga
      841 ctgtcgaaaa ttgccaagtc gggagataaa gatgcaaaac gggggattga aattttgtcg
      901 catctgaagg aacattttga aaattttggc aatgctagag acctgaatct gaccggtgaa
      961 gagaaaaaat atattgatga cctgtttctt cttacaataa agccggtgtt atatgtatgc
     1021 aatgtggacg aaaaatcggt gcacaccggc aataaatatg ttgaacaggt taaagagtat
     1081 cttaaaggga aggatgctga ggtgctggtg attgcaggta aaatggaagc tgagattgcc
     1141 gaacttgaaa acgatgaaga ccggatggct ttcctgaatg atgcgggtct tgatgaacct
     1201 ggtgtcaata agctggtaag ggcagcctac cgattgctta acctgattac attttttact
     1261 gtgggcccaa aagaaatcag agcatggaca attaaaaaag gaactaatgc ccagcaggct
     1321 gcaggtgtta ttcattctga ccttgaaagg ggatttatca gggctgaagt gatgaaatac
     1381 aacgactttg ttacactagg ctctgagcat gcctgcaaag aggccgggaa atttcatgtg
     1441 gaagggaaaa attatattgt agaagacgga gatatacttc atatccgttt taacgtttaa
     1501 aggatatgaa cacccgtatc atcatactat ccttgctaat atttctgtta tctgctgttt
     1561 ctttttcaca ggttaccagg gatgaaaagc ttgcagagga agtaaaaaag tatggtgagg
     1621 ctgaggtagt tatatcatat ccgggcagga atgctataga gctgattacc agaaatgttt
     1681 caatcacggc agtgaaggat aaaaaagttt acatttcttt gacggctgcc gctttaaact
     1741 ggtttttatc gcaaaggttt gattatgaga ttattgaaaa agagcctgca aaaggtatca
     1801 tatctgcttc ttcggtaagc ggtgcaatgg agtgggacag ctatcctact tacacccagt
     1861 atgattcgat tatgaggaaa tttcaggcag actatcctca actatgccag ctcgatacta
     1921 taggaacaag tatattgggg cgccttgttc ttgttctgaa aatttctgat aatgtaaacg
     1981 atgatgaacc cgagcctgag gtattctatt catcaacaat acatggcgat gagctgggag
     2041 gctttgtctt gatgttaagg ttggcagatt atcttctaaa aaattacaat acagattcaa
     2101 gagtcagaaa tcttgtcgac aaccttgaaa tatggataaa cccacttgcc aaccctgacg
     2161 gaacctacag gaccgggaat gaaataatct ctcccacaag gtcgaatgca aacggtatcg
     2221 accttaaccg aaattttccc gaccctatga atccttctat tgttcctgag aaggaaaaca
     2281 tcgacatgat ggcttttatg cgtaaaagaa ggttcgtact ctctgctaat tttcattccg
     2341 gcttcgaagt ggtaaatttt ccgtgggaca gatggctcag taaatatcat gctgatcatg
     2401 actggtttta ttatataagc agaaaatatg ccgatacggt acatgtatat tctggcccgg
     2461 cctacatgaa ttattatgac aacggggttg taagaggtgc tgaatggtat ataatatatg
     2521 gagggaggca ggatttcata acctgggagt tacagggcag agaggtaacc atagaactgg
     2581 atgacataaa gcagactcct gcagcacagc tggagcttct ctggcagtat aaccggcaat
     2641 ccctgattgg ttatctggaa aatgctcttt atggcattca cggaagggtg gtagatgcag
     2701 ttactaagaa acctgtacct gctaaaatat atattgccgg gcatgataaa gacagctccc
     2761 atgtttatgc tgattcgttg acaggatatt ttacccggtt tcttgcgcag ggtagctgga
     2821 acctgacttt ttcagccaca gggtacaaag acactgttgt tcagggagta aacgttgtaa
     2881 acaaccagcg tacagatctg tatgttgaga tgaaatcgac tcctactgat atcgattcag
     2941 tcaacccgga aaaaccactt ctgtttccca atccatcatc tgaaataatt gaatgccggt
     3001 tgcctgattc tatcacaggt caggtaaaag tctcagtaat aaacagcaat ggtgtaaaaa
     3061 tacttgagta cttgacttca gatagaccgg tgatattaaa tgtcgcttcc ctcgatgcag
     3121 gtgtatatac tgtagttttt aaatcaacgg tgaccgggca ctcagcaagc agccgatttg
     3181 ttgtatattc aagacattat t
//
LOCUS       DAOSNB010000290         3174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_30496_length_3174_cov_3.006412, whole genome shotgun sequence.
ACCESSION   DAOSNB010000290 DAOSNB010000000
VERSION     DAOSNB010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30496_length_3174_cov_3.006412"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1762
                     /locus_tag="PLG42_10040"
     CDS             416..1762
                     /locus_tag="PLG42_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOU31589.1"
                     /translation="MNTRKLNCLFLLLLLAFSLSSNAQLPKETEAQKAQRMKWWTEAR
                     FGMFIHWGLYALPARHEWVKNRERMTNEQYQKYFEMFNPDLFNPREWARMAREAGMKY
                     VVLTAKHHEGFCLWDSKYTDYKCTNTPFGRDIIKEFVEAFRAEGLKVGFYYSLIDWHH
                     PDYTIDRNHPLRQETDSAYERLNRGKDMSKYREYMKNQVRELLTNYGEISIIWFDFSF
                     PGKNGKGRNDWDSEGLLKLARTLQPNIIVDDRLDLHDVEGGWDFVTPEQVKVDKWPEY
                     NGKKVPWETCQTFSGSWGYYRDEYTWKSTAQLLELLIESVSKGGNLLLNVGPTGRGTF
                     DYRAQERLKAMGEWMKFNSRSIYGCTQAPDEFKAPANTLLTYNPSTKRLYIHLLAYPL
                     NRIILENMTDKVKYAQFLHDASEIRYSTVQGTNNLALQLPVLKPPVEIPVIEIFLK"
     gene            1773..2456
                     /locus_tag="PLG42_10045"
     CDS             1773..2456
                     /locus_tag="PLG42_10045"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOU31590.1"
                     /translation="MNLRNTGFLTILLVILLTSSQSGGCRNESAGKKQITAEEVLPSD
                     STCIFDGKTLDGWEITNFGTQGPVDVSGGKIYLGMGDGCTGITYCKSFPEINYEVTLE
                     AMRVEGNDFFCGMTFPVGKDPCTLIVGGWGGTVVGLSSIDGKDASENETTRYMKFDSD
                     RWYKIKLRVTEEKIEAWIDDELIVNFTIDGKRLSIRPEVELSKPFGIASWQTTAALRN
                     IRVRRITEE"
     gene            2546..>3174
                     /locus_tag="PLG42_10050"
     CDS             2546..>3174
                     /locus_tag="PLG42_10050"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOU31591.1"
                     /translation="MCRFNTIRFFTIFLCFMMGICCSAESDTTRHRFAGVNGQYGFII
                     PHSVVIEPVSHSNPYGVLLETGFMSKSYNSWKVFNSWWTAGLQTGYYSFDNPDTLGGA
                     FILAGFAEPVLFSNKRFVMTVRAGGGLSYHTKIYDASANPANQFFCTRISFPVYAAMR
                     FKYRIWSNIYLTLSGYYNHISNGGVKQPNYGMNFTTMALGLDYFSPPLSP"
BASE COUNT          946 a          657 c          742 g          829 t
ORIGIN      
        1 agtgtccggg ctgcggccgc atttactggg aaggatcaca ctttcagagg atgaaggagt
       61 ttattaaggt ttttactgaa ggttcaggaa aataattttc agtttaaaag ctatatgcta
      121 tctttaaaca gaacagaata taaattaagc tttttatctt ctttttgccc ctcccgacct
      181 ccccagatgg ggaggagttg cccctcccgg cctccccaaa tggggaggag ttgcccctcg
      241 aggttaactg tgatgcacac tataaaatcc ttcccccacc tgggggaagg ttgggatggg
      301 ggcagataca aggccgtgat gggggtatta gacaggtaag acaaaaactt aaaaaacaat
      361 taaattgtat aaattcaaac aaatatatta cggacttaac ctaaatctta ataccatgaa
      421 taccagaaaa ctaaactgtt tgtttttgtt attactgctg gctttttcac tgagcagtaa
      481 tgcccagctt ccaaaagaaa ctgaagctca gaaagcacag cggatgaaat ggtggaccga
      541 agcaagattc ggtatgttta ttcattgggg gctttatgca ctgcccgccc gccacgaatg
      601 ggtaaaaaac cgtgaaagaa tgaccaatga acagtaccag aagtactttg aaatgttcaa
      661 tcccgatctg ttcaatccgc gtgaatgggc acgtatggcc agggaagcag gaatgaaata
      721 tgtcgtgctg acggcaaaac atcacgaagg cttctgtctg tgggactcaa agtacaccga
      781 ttacaagtgc accaacacgc cgttcggaag agatatcatt aaggagtttg tggaagcttt
      841 cagagccgaa ggcctgaaag tgggatttta ctattcactt atcgactggc atcatcccga
      901 ttatacaatc gacaggaacc atccattgcg acaggaaacc gactcggcgt atgagcgcct
      961 gaacaggggg aaggacatga gcaaataccg tgaatacatg aagaatcagg taagagaatt
     1021 gctaacgaat tatggtgaaa taagcattat ctggttcgat ttctctttcc cgggcaaaaa
     1081 cgggaaagga agaaacgact gggactcgga aggcctgctc aaacttgccc gtacccttca
     1141 gccaaacata attgttgacg accggctcga cctccacgat gttgaaggcg gatgggactt
     1201 tgtcactccc gaacaggtga aagttgataa atggcccgaa tataacggca aaaaagtgcc
     1261 atgggaaacc tgtcagacct tctctggctc gtggggttat taccgcgatg aatatacatg
     1321 gaagagtacg gcccagcttc ttgaattgct tatcgaatcg gtaagcaagg gtggcaacct
     1381 gctccttaat gtaggtccga caggccgtgg taccttcgat taccgtgcgc aggagagact
     1441 gaaagctatg ggcgaatgga tgaaattcaa cagccgctca atatatggct gcacacaggc
     1501 acccgacgaa ttcaaagcac cggccaatac gctgcttaca tataaccctt caacaaaacg
     1561 cctgtacata catcttcttg cctatccgct taacagaatt atacttgaaa atatgacaga
     1621 taaagtgaaa tatgcacaat ttctgcacga tgcttccgaa ataagataca gcacagtaca
     1681 gggcacaaat aaccttgcac ttcagcttcc ggttcttaaa ccccctgtgg agataccggt
     1741 tattgaaatt tttcttaaat gattcattga ttatgaacct cagaaacaca ggctttttga
     1801 caatattgtt ggtaatcctg ctaacttcat cgcaatcggg tgggtgcaga aatgaaagtg
     1861 caggcaagaa acagattacc gctgaagaag tccttccatc tgactctacc tgtatttttg
     1921 atggtaaaac cctcgacggc tgggaaataa cgaatttcgg aacacaggga ccggttgatg
     1981 tgtcgggtgg aaagatttac cttggcatgg gcgatggctg tacaggaata acctactgta
     2041 aaagttttcc ggaaataaat tatgaagtga cactcgaagc catgcgcgtt gaaggaaacg
     2101 attttttctg cggcatgaca ttcccagtcg ggaaagaccc ctgtacactt atagttggag
     2161 gctggggtgg cactgttgtc ggactgagca gtattgatgg gaaagatgcc tcggagaatg
     2221 agacaactcg atatatgaaa ttcgatagcg acagatggta taaaataaag ctgagggtaa
     2281 ctgaagaaaa gatcgaggca tggatagacg atgaactgat agtgaatttt acaattgacg
     2341 ggaaaagact ttcaatacgt cccgaggtgg agctttcaaa accattcggg atagcatcat
     2401 ggcagacaac cgcagcgctc aggaacatta gagttcggag aattacagaa gagtgatgta
     2461 aatatcactt tgtgttattt atcctgagat cctgatttca ggttctcttc agctggaaat
     2521 ctgtatgttt tgaattgttc tgactatgtg tcggtttaac acaatcagat ttttcaccat
     2581 ctttctttgt ttcatgatgg gcatctgttg tagtgcagag tcggacacga cccgccaccg
     2641 ttttgcaggc gtaaatggtc agtacggttt cattattcct cattcagtgg ttatagaacc
     2701 agtttcgcat agcaatccat atggtgtttt gttggagaca ggttttatga gcaaatcata
     2761 caacagctgg aaagttttta attcatggtg gacagcaggt ctgcagactg gctattacag
     2821 ttttgataac cccgataccc ttggaggtgc gtttatcctg gcaggatttg cagaaccggt
     2881 tctattctcc aataaaagat ttgtaatgac agtaagagca ggcgggggac tctcgtatca
     2941 tacaaaaatt tatgatgcat ccgccaatcc cgcaaatcag tttttctgca cgcgaatcag
     3001 tttcccggtt tatgcagcca tgagattcaa ataccggata tggtcaaata tatatctcac
     3061 attatcaggt tattacaatc acatatcaaa cggaggggtg aaacaaccta attacggtat
     3121 gaactttaca acgatggcac tgggattaga ttatttttcc cctcctcttt ctcc
//
LOCUS       DAOSNB010000291         3125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_31175_length_3125_cov_4.073616, whole genome shotgun sequence.
ACCESSION   DAOSNB010000291 DAOSNB010000000
VERSION     DAOSNB010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31175_length_3125_cov_4.073616"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1112)
                     /gene="xerD"
                     /locus_tag="PLG42_10055"
     CDS             complement(207..1112)
                     /gene="xerD"
                     /locus_tag="PLG42_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOU31592.1"
                     /translation="MSPGINWSDAVRDFETYLRLEKSLSSNSIEAYLHDITSLREYFS
                     GEGRNKKPAEITYNDLQDYIHWLNSSNPNPRTQARMLSGIRAFFRYLLIEGVISENPS
                     ALIESPKIGFHLPEVLSLAEIEKLIDTIDLSKPEGHRNKAIIETLYGAGLRVSELVNL
                     RLTDIHKAEGFLVITGKGNKQRMVPIGSKALKAIELYLEQRRRFRVIHDENIVFLNRR
                     GRKLTRVMIFTIIKDLARKAGIRKKISPHTLRHSFATHLIEAGADLRAVQEMLGHESI
                     LTTEIYTHIDRTFLRDTLVMFHPRA"
     gene            1270..1701
                     /gene="aroQ"
                     /locus_tag="PLG42_10060"
     CDS             1270..1701
                     /gene="aroQ"
                     /locus_tag="PLG42_10060"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOU31593.1"
                     /translation="MKIQIINGPNLNLLGKREPEKYGYTSFEDFLAELREKYPSVEIE
                     YFQSNVEGEIINRIHETGFTYDGIILNPGGYTHTSVAIADAVAAVKCPVVEVHITNVA
                     AREEFRHNSLVGRFCRGSIIGFGLLGYLLAVEAIIHLAKKV"
     gene            1698..>3125
                     /gene="pyk"
                     /locus_tag="PLG42_10065"
     CDS             1698..>3125
                     /gene="pyk"
                     /locus_tag="PLG42_10065"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOU31594.1"
                     /translation="MMKKKRTKIVATISDKRCDPEFIRRLYEEGMDVVRINSAHLDLE
                     GALKIIRNVRQVSDKIAILLDTKGPEIRTTICDAPIYLKKGEIVKISGEPGKNTERGN
                     ICVTFPGFADEVPVGTSVLIDDGEIELKVTGKEGNSLECTVVDDGILGSRKSVNVPGV
                     RICLPSVTPKDRLFIEMAAKEDIEFIAHSFVRNRQDLLDVKAILEKAGSEIKLIAKIE
                     NQEGVDNIDEILDEAYGIMVARGDLAIEIPYEKVPVIQRMIINKCIRRRKPVIVATQM
                     LQTMINNLRPTRAEISDIANAIYSHTDAMMLSGETASGKYPVEAVQTMTKVALEVENH
                     TEQFLDIPPGTQAGEISGHLARIAVETSVKVGAKGIIADTAEGRTIRDMAAFRGRGLI
                     LAQCYKKRTMRELALSYGVYADYQEKKKSVDMFIHTALGKLVKTHDLSDNDIVIVLAG
                     NFSGGRGFSFIEVGTVDYLKNRVTYV"
BASE COUNT          931 a          618 c          701 g          875 t
ORIGIN      
        1 gagatatttt gatacctttt taccaggaaa cagaagatgc atcagtccgg caaagaaaaa
       61 tcccagcagc agatatggtg acatctcaag aaaaattgcc cacagttcag ttccgaattt
      121 taccagataa ttcattttga gtttatttct tagtatataa acaaaattga cagccttctg
      181 tttatttcaa catggaatta ttctatctaa gctctcggat gaaacatcac tagcgtgtcc
      241 ctcaggaaag tcctgtcgat atgggtatat atttcagttg tgaggattga ttcgtgtcct
      301 agcatctcct gtacagccct gaggtcagca ccggcttcaa tcagatgtgt ggcaaacgaa
      361 tggcgcagag tgtgaggact tatcttcttc ctgattcctg ctttccgagc aaggtcttta
      421 ataattgtaa atatcattac ccgtgtaagt tttcttcccc tgcgattaag aaagacaata
      481 ttttcatcat gtattacccg gaaccgcctt cgctgctcaa gataaagctc tattgccttc
      541 agtgctttgc ttcctatagg caccatacgc tgtttatttc cttttcctgt aatcaccagg
      601 aaaccttcag ctttgtgaat atcagtcagt cttaggttta ccagttcaga taccctcaat
      661 cctgcaccat agagggtctc aataattgct ttgtttctgt gaccctccgg cttgctgaga
      721 tctattgtat ctataagctt ttcaatttct gctaacgata aaacttcagg cagatgaaat
      781 ccaattttag gcgattcaat cagcgctgag ggattctcgg aaatgacacc ctcaatcagc
      841 agatatctga aaaatgccct tatacccgag agcatccttg cctgggttct ggggttggga
      901 ttgcttgaat taagccaatg tatataatct tgcagatcgt tgtatgtaat ttcagccggc
      961 tttttgtttc ttccttcacc tgaaaaatat tccctcagac tcgttatgtc atgaagatat
     1021 gcttctatac tgttagacga cagagatttt tcgagtctga gataagtttc aaagtccctt
     1081 acagcatcac tccagttaat tccaggtgac atacaaaatc tttttaacag cgttcacgtt
     1141 atcaatacgc tggtgtaaaa acattgatgc aaaattatta aaatggtaca tattattgat
     1201 tttttgcgct attttgcatt ataaacacaa actgtttttt aagtaggttt ttataaaaat
     1261 attccggaaa tgaaaataca gataattaac ggccccaatc ttaatctgct gggtaaaagg
     1321 gaaccggaaa aatacggata tacttcattt gaagattttc tggctgaatt gcgagaaaaa
     1381 tatccttcag tggagattga atattttcag tcgaatgttg aaggcgagat aattaacaga
     1441 atacacgaaa cggggttcac ttatgacgga attattctta atcctggcgg atatacacat
     1501 acatcggtcg ccatagctga cgcagttgca gcggtaaagt gccctgtggt tgaggttcat
     1561 ataacaaatg ttgctgcacg cgaggagttc aggcataaca gtcttgtggg cagattctgc
     1621 cgcggaagca ttatcggatt cggccttctt ggttatctac ttgcagttga agctattatt
     1681 catttagcga agaaagtatg atgaaaaaga aaaggacaaa aattgttgct actatatccg
     1741 acaagaggtg tgatcctgag tttatcagga ggctgtatga ggaaggtatg gacgtggtaa
     1801 gaataaactc ggcgcacctt gatctggagg gagcacttaa gattataaga aacgtacgtc
     1861 aggtgtccga taaaatagca attctgctgg acaccaaggg tccggagata agaaccacaa
     1921 tatgcgatgc ccctatatat ctgaaaaaag gagaaattgt aaagatttca ggtgaaccgg
     1981 gaaagaatac agagcgggga aatatctgtg ttactttccc tggttttgca gatgaagttc
     2041 ctgtgggaac ctccgtactt attgacgatg gcgaaattga actgaaagtc acaggaaaag
     2101 agggcaactc tctcgaatgc acagttgttg atgatggcat actcggttcc aggaaaagcg
     2161 tgaacgtccc tggtgtcagg atctgtcttc cttcagttac gccgaaagac cggctcttca
     2221 ttgagatggc cgcaaaggaa gatattgagt ttatcgctca ctcttttgtg cgtaaccggc
     2281 aggatttgct cgatgtaaag gcaatactgg agaaagccgg cagcgaaata aaattaatag
     2341 ccaaaataga aaaccaggaa ggggttgaca atatcgacga aattctggat gaagcctatg
     2401 ggataatggt tgcaaggggt gatctggcaa ttgagatccc atatgagaag gttccggtta
     2461 ttcagcgtat gataatcaat aaatgtatcc ggcgaaggaa accggttatc gttgctacac
     2521 agatgcttca gacaatgatc aataaccttc gtccgacacg tgcagagata agcgatatcg
     2581 caaacgcgat ctattcgcat accgatgcca tgatgctcag cggcgaaacc gccagcggga
     2641 aatatcctgt tgaggcagta cagacaatga caaaagttgc ccttgaagtg gaaaaccata
     2701 ctgaacaatt ccttgatata ccgccgggta cacaggcagg tgagatttca ggccaccttg
     2761 ccagaatagc tgtcgagaca tcagtgaagg tgggagcaaa aggaataatt gctgataccg
     2821 ctgaaggacg taccatcaga gatatggctg cattcagggg cagaggactt atccttgccc
     2881 agtgctacaa aaagagaaca atgagggaac ttgctctctc atacggggtc tatgccgact
     2941 atcaggagaa gaagaaatcg gtcgatatgt ttatccatac agctcttgga aaactggtta
     3001 aaacacatga tctgtccgat aatgatatcg tgattgtcct tgccggtaat ttctcgggcg
     3061 gaagaggatt ctccttcatt gaagtgggta cggtagacta cctgaaaaac agagttacct
     3121 atgtc
//
LOCUS       DAOSNB010000292         3091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_31619_length_3091_cov_4.512187, whole genome shotgun sequence.
ACCESSION   DAOSNB010000292 DAOSNB010000000
VERSION     DAOSNB010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31619_length_3091_cov_4.512187"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2828)
                     /locus_tag="PLG42_10070"
     CDS             complement(<1..2828)
                     /locus_tag="PLG42_10070"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOU31595.1"
                     /translation="MKSKYLVKYIILFFIILLCLSYSCKKPSFDQLLREGFINPPDSA
                     KPGVYWYFMDGNIDKKAITEDLEAMKQAGIGYALFLEVNVGIPRGSVDFMSEEWMELF
                     AHAVREAERLGIKIILGSGPGWAGSGGPWVKPEQSMMHIVAADTVVTGPVLFDAQLPL
                     PAPKKPFFGENTLTPELKELRDNWYEDIRVLAFPAPDKQNKIENLDEKAFYYRAPYTS
                     QPDVEPFILPFDSTKETTEPSVKKDKIIDLTLIYQGNGRLTWNVPPGRWVIIRFVKRN
                     NGAVTRPAPLPGLGFESDKFDTAALGEHFRAFTENLIKKVNKGRKSSSPGWTMLHIDS
                     WEMGAQNWSHRFIDEFIARRGYDPVLYLPVFAGYTVNSAEESERFLWDVRQTASELIV
                     ENHAGYFKSLGRKYGFLLSIEPYDMNPASDFDLGAVADIPMGEFWTKGHGFNSSFSCI
                     EATSIGHVTGKPVIAAEAFTADRTEAWKMYPGNMKNQTDWALAMGINRFIFHTFAHKP
                     HGSKYLPGMTMGPYGVHWDRGQTWWPLVSEYHKYLSRCQLMLMQGVSAADILFLTPEG
                     APNVIVPPASALDGNDTISDKKGYSFDACSPSYLIKNAFVVNGRVTFPSGASYTLLVL
                     PRLNTMTPELLEKINELLKTGATVAGWMPVKSPSLAKYPECDKEVKKLSETIWGINWS
                     VGKSQIKYLNGTLFKLEEYIKEPYNPSPDDNYDIYPSYETLVSLMRALGREPDFTCKS
                     GNIRWHHKKLTEKEIYFISNRTDETISDTCYFRDGSDDAELWDPLTGDIRKLKNIFYR
                     GWVTEVPIKLEPYQSFFIVFNKTNAATFRIRTGTENDFPEKKELFSLDRNWSVYFDPS
                     WGGPGEVAFDSISEWTERPEPGIRYYSGTAVYRCSFSMPEGKLNKKANDYFIKINLSN
                     SMAKVKLNGKTAGIIWTSPGELKITDYL"
     gene            complement(2818..>3091)
                     /locus_tag="PLG42_10075"
     CDS             complement(2818..>3091)
                     /locus_tag="PLG42_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOU31596.1"
                     /translation="SGGELPAAVITDAVVRLLPGAISDEQSALTDSFQDDLLAPPVYT
                     RPAEFLGWKVPEILLSGNTPEIEKWRLEQSIERTKRLRPDLLKDEK"
BASE COUNT          765 a          702 c          679 g          945 t
ORIGIN      
        1 ttcaaataat ctgtgatctt cagctctccc ggcgatgtcc atattatacc tgccgttttg
       61 ccgtttagct ttacttttgc cattgaattt gaaaggttta ttttgatgaa ataatcattt
      121 gcctttttgt taagtttccc ctcaggcata ctgaaactgc atctgtaaac tgctgtcccg
      181 gagtagtatc tgatgccggg ttctggtctt tcagtccatt ctgatattga gtcgaatgcg
      241 acttcgccgg gtccacccca tgaaggatca aaataaacac tccagtttcg gtcgagactg
      301 aaaagctcct tcttttcagg gaaatcattc tccgtgccgg ttcttattct gaaagtagca
      361 gcattggttt tattgaacac aatgaagaaa ctctgataag gttccagttt aatcggaact
      421 tctgtcaccc atcctctgta gaatatgttt ttgagcttcc tgatatctcc ggttaatgga
      481 tcccagagct ctgcatcgtc tgaaccatcc ctgaagtaac aggtatctga aatagtttca
      541 tctgtccggt ttgaaataaa atatatctct ttctcagtca attttttatg atgccatctt
      601 atatttcccg atttgcaggt aaaatcaggc tcccttccaa gagccctcat cagtgaaacc
      661 agggtctcgt atgacggata aatgtcataa ttatcatctg gtgaagggtt gtacggttct
      721 ttaatatatt cttcgagttt aaaaagagtt ccgttgagat attttatctg gcttttgccg
      781 acgctccaat ttattcccca gattgtttcc gaaagttttt taacttcttt gtcgcattca
      841 ggatattttg caagtgatgg cgatttcact ggcatccagc ctgcaactgt tgcccctgtc
      901 tttaatagtt catttatctt ttccagtaat tcgggtgtca tggtgtttaa acgtggcaat
      961 acaagcaatg tataggatgc gcctgatggg aaagtaactc gtccattaac gacaaatgca
     1021 tttttgatca ggtatgaagg tgaacaggca tcgaatgagt agcctttttt atcggatatt
     1081 gtatcattac catcgagtgc tgaagcgggt ggaacaatta catttggtgc accttcaggt
     1141 gtaagaaaca aaatatcggc agctgacaca ccctgcatca gcataagctg acatcgtgag
     1201 agatacttat ggtattcact caccagtggc caccatgtct ggccgcggtc ccagtgcaca
     1261 ccataaggac ccattgtcat accaggcaga tatttgcttc catggggctt atgtgcaaag
     1321 gtgtgaaaga taaacctgtt tattcccata gctagagccc aatcggtctg gtttttcatg
     1381 ttaccaggat acattttcca tgcttcagta cggtcggcag taaaggcttc tgcagcaatt
     1441 acgggtttac ctgtaacatg tcctatcgat gttgcctcta tgcagctaaa tgatgaattg
     1501 aacccatgtc cttttgtcca gaactctccc ataggaatgt cggctacagc tccgaggtca
     1561 aagtctgatg caggattcat gtcgtagggc tctatcgaaa gaaggaatcc gtatttgcgt
     1621 ccgagagact taaaatatcc tgcatgattt tcaacaataa gttcactggc tgtttgccgt
     1681 acatcccaca gaaatctctc gctttcttct gccgagttaa ctgtgtaacc tgcgaatact
     1741 ggcagataga gaacggggtc gtaccccctt cgtgcaatga attcatcaat aaatctgtgg
     1801 ctccagttct gggcacccat ctcccagctg tctatgtgca gcatggtcca gcccggtgag
     1861 gacgatttcc ggcctttgtt tacctttttt ataagatttt ctgtaaaagc tctgaaatgt
     1921 tcacccagcg cggcggtgtc gaatttgtcc gattcaaaac ccaatccggg aaggggtgcg
     1981 ggacgtgtaa cagctccatt attgcgtttt acgaaacgga ttatcaccca tctgcctggt
     2041 ggtacattcc atgttagtct tccgttgccc tggtatatca gggtaaggtc tataatctta
     2101 tcctttttta ctgatggctc tgtggtttct tttgtactgt cgaaggggag gataaagggc
     2161 tcaacgtccg gttgcgaagt gtatggagcc ctgtaataaa aggctttttc gtcaaggttt
     2221 tcaattttat tctgcttgtc tggagcagga aaggcaagca ctcttatgtc ctcgtaccag
     2281 ttatctctga gttcctttag ttcaggtgta agtgtatttt caccaaagaa aggttttttt
     2341 ggtgctggta gaggaagctg ggcatcgaat aatactggac ccgtaacaac ggtgtcggcc
     2401 gccacaatat gcatcatact ttgttcgggt ttaacccatg gtccgccgct gccagcccaa
     2461 cccggcccgg aacccagtat tatttttata ccaagccttt ctgcttctct cactgcatga
     2521 gcaaaaagtt ccatccattc ttcgctcata aaatcgacac taccgcgtgg aatgccaaca
     2581 ttcacctcaa gaaacagcgc atagcctatc ccggcctgtt tcatcgcttc aaggtcttct
     2641 gtaatagctt ttttatcaat attgccatcc atgaagtacc agtaaacacc cggcttcgcc
     2701 gagtcaggcg ggttgataaa accctcccgc aacagctggt cgaatgatgg ctttttgcat
     2761 gaatagctca ggcaaagcag aatgatgaaa aaaagaatta tgtatttaac aaggtattta
     2821 cttttcatct ttaagcagat cgggtctcag acgtttggtt cgttcgattg attgttccag
     2881 tctccatttt tctatttcgg gtgtattgcc tgaaaggagt atctcgggca ctttccatcc
     2941 caagaattca gcgggtcggg tatatactgg tggagcgagg aggtcatcct gaaaagagtc
     3001 agtgagagcc gactgttcat cagagatggc cccagggagg agtctaacca cagcgtcggt
     3061 tatgacagcg gcaggcagct cgcctcccga g
//
LOCUS       DAOSNB010000293         3080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_31755_length_3080_cov_2.973554, whole genome shotgun sequence.
ACCESSION   DAOSNB010000293 DAOSNB010000000
VERSION     DAOSNB010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31755_length_3080_cov_2.973554"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLG42_10080"
     CDS             complement(<1..1048)
                     /locus_tag="PLG42_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOU31597.1"
                     /translation="MPKTQNIDPKEVRKPGFITFGKIPVNQYSKTIADERGNYSDADF
                     IRIYRDMVIIREFETMLNSIKTKNEYRGIQYNHPGPAHLSIGQEAAAVGMAYTLGTDD
                     YIFGSHRSHGEIIAKGLSAINKLDEEVLYSLMKNYFGGNTLRVVEKDFTGDIKELAVH
                     FLLYGALAEIFARETGFNKGLGGSMHAFFTPFGIYPNNAIVGGSGDISVGAALYKKIN
                     NKSGIVVCNIGDASMACGPVWEGICFATMDQYRKLWSGEYQGGLPLIFNFMNNFYGMG
                     GQTYGETMGFGILARVGAGVNPDMMHAERIDGYNPLAVIDAFRRKRKILEEKKGPVLL
                     DTLTYRFSGHSPSDA"
     gene            complement(1256..2506)
                     /locus_tag="PLG42_10085"
     CDS             complement(1256..2506)
                     /locus_tag="PLG42_10085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU31598.1"
                     /translation="MATPVIMPKQGQSVETCVITTWFKHKGDYVKTGDLLCSYETDKA
                     SFDLESPAEGTILDIFFNDGDEVPVLTAIAVIGTEGEDVTSFRPGATSKTEKAGETAP
                     QLSESLYAISAEVTTPSMPEGKIRISPRARNLALKEKIDITRIKGTGPAGRIIIRDIE
                     NELKARMQQPEPQFLNVESPSADYTDKPLSNIRKIIAGAMHASLQNSAQLTHHMSADV
                     RKLLEARKKIKEEIQAGKNSPDITLNDMICYCVIKALKKHPEVNSHFLGDKIRTFSDV
                     HLGLAVDTARGLMVPAIRNAGSMSLTVLSRELKEAAEACRRGSIDPELLKSTAASFTV
                     TNLGAYGVEMFTPVLNLPQVAILGVCTITHRPADLGNGVMGFIPFIGLSLTYDHRAID
                     GGPATLFLKEIKEQIETFNLPTLL"
     assembly_gap    2650..2659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2660..>3080
                     /locus_tag="PLG42_10090"
     CDS             <2660..>3080
                     /locus_tag="PLG42_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU31599.1"
                     /translation="PRYKELYHTLRKHILEGIYREGDLLPSENELCSIYGMTRPTVRQ
                     ALALLAGDGLIRKHQGKGSIVHRMPREIGILSVAGTTSAVGDRGLKTDIIVKPKVIPW
                     PGEFMFPLSETEKESGCIYMERLRLLEGTPIFYDINYI"
BASE COUNT          756 a          651 c          666 g          997 t
ORIGIN      
        1 aggcatcgga aggtgaatga ccgctaaagc gataggtaag tgtatcgaga agaaccggac
       61 cttttttctc ttcaagtatt ttccttttcc ttcggaaagc atctatcact gcaagagggt
      121 tatagccatc aattctttcg gcatgcatca tgtcaggatt cactccggca ccaaccctgg
      181 caagaatgcc gaagcccata gtctctccat aagtctgtcc gcccattccg taaaaattgt
      241 tcatgaaatt gaaaataagc ggaagccctc cctgatattc accgctccag agttttctgt
      301 attggtccat agtagcaaaa catattcctt cccataccgg tccgcaggcc atggatgcat
      361 caccgatgtt gcatacaaca atgcctgact tgttatttat ttttttatag agggcagctc
      421 ccacagatat gtcgccagaa cctcctacta tggcattgtt cggatagatg ccgaatggag
      481 taaaaaatgc gtgcatcgaa cccccgagtc ctttgttgaa accggtttcg cgggcaaaaa
      541 tctcagccag cgctccatag agaagaaaat gcactgcaag ttcttttata tcacctgtaa
      601 agtctttttc taccactcta agagtgtttc cgccgaaata gtttttcatt aacgaataga
      661 gtacctcttc atccagtttg tttattgccg acagaccttt ggcaatgatt tcgccgtgac
      721 ttcggtgaga accaaaaata taatcgtcgg ttccgagagt ataggccatg cctactgctg
      781 cagcctcctg accgattgaa aggtgcgccg gccctggatg gttatactga atgccacggt
      841 attcgttttt ggtcttgata ctgttgagca tcgtttcaaa ctcacggatt atcaccatgt
      901 cgcggtaaat ccttatgaaa tcggcatcgg agtaattacc tctctcatcg gcaatggttt
      961 tgctgtactg gtttaccggt atcttaccga aagtgatgaa cccaggtttt ctgacttctt
     1021 tgggatcgat gttttgtgtt tttggcatat taatttactt tatttcattt tactgatttt
     1081 atcctttaat tcaatctgta tttactcatt acttattttc ttcctgttca ttcacaagtt
     1141 cttgtttctt atttcttttt agctatcatc tggttatttt ccggtttctc tcttttttat
     1201 ctctcctgca aaatgttgag agagaaaatg ttctttgtta cgatatgcta tgattttaca
     1261 acaaggttgg gaggttaaat gtttcaattt gttctttaat ttctttaagg aagagtgtag
     1321 cgggtccgcc gtcgattgcc cggtggtcgt atgtgagtga aaggcctata aatgggatga
     1381 agcccattac tccgttgccc aggtcggcag ggcggtgggt tattgtgcac acacccagta
     1441 ttgccacctg cgggaggttt aagacgggtg taaacatttc cacgccgtag gctccaaggt
     1501 ttgttacagt gaaagatgcg gctgtgcttt tgagcagttc ggggtcgatg cttccccggc
     1561 ggcatgcctc tgctgcctcc tttagttctc tggagagaac tgtcaggctc atgctgcctg
     1621 cattccttat tgcaggaacc ataagaccgc gggccgtgtc aacagccagc ccgagatgca
     1681 catctgagaa agttcttatt ttgtcgccga gaaaatggct gttaacttca ggatgttttt
     1741 tcagggcttt gataacacag taacatatca tatcattcaa agtgatatca ggggaattct
     1801 ttcctgcctg tatctcttcc tttatttttt tcctggcttc caggagtttt ctaacatcag
     1861 cgctcatgtg gtgagtaagc tgtgcagagt tctgcagtga ggcgtgcatt gcaccggcaa
     1921 ttattttcct tatgtttgaa agaggtttgt cggtgtaatc tgcagaaggt gattcaacat
     1981 tcaaaaactg tggttcaggt tgctgcattc ttgcttttaa ttcattttca atatctctga
     2041 tgatgattct ccctgctggt ccggtgcctt ttattctggt aatatctatt ttctctttta
     2101 aagcaaggtt tctggcacgt ggtgaaattc ttatttttcc ttcaggcatt gaaggggttg
     2161 taacttctgc agagatggca tacagcgatt cgcttagttg aggagcagtt tccccggctt
     2221 tttctgtctt gctggttgct cctggccgga atgatgttac atcctcacct tcggttccaa
     2281 tcactgcaat ggcagtaagt accggcactt catcaccgtc gttgaaaaat atgtcgagta
     2341 ttgttccctc agctggcgat tcgaggtcga atgaggcttt gtctgtttcg tacgagcaga
     2401 gaagatctcc tgttttgaca taatcgcctt tatgtttgaa ccacgtggtt attacacatg
     2461 tttcgaccga ctgtccctgt tttggcataa ttaccggtgt tgccattgct tttaattttt
     2521 aacttgttaa gatatgtttc aaatcgatct tattaaaata aggttatatg gtatgtgcaa
     2581 aaataattaa ttaaacttgt atttacaagt aaaatttaat atatttacac tttcaaataa
     2641 aaaatgatan nnnnnnnnnc cacgatataa agaattatat catactctga ggaagcatat
     2701 tcttgaaggc atttacaggg aaggtgacct gttgccctcc gaaaatgagc tttgcagcat
     2761 ttacggcatg actcgtccta ctgtaaggca ggctcttgct ttgcttgccg gagatggatt
     2821 aatacgcaaa catcagggga agggaagcat tgtccacaga atgccaagag agataggaat
     2881 cctttcagtc gcaggcacta catcggctgt aggagataga ggccttaaga ccgatataat
     2941 tgtaaagcct aaagtaattc cctggcccgg tgaatttatg ttccctcttt ctgaaaccga
     3001 gaaggaatca gggtgtattt acatggaacg gctcaggctc cttgaaggaa cccctatctt
     3061 ttatgatatt aattatatag
//
LOCUS       DAOSNB010000294         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_31769_length_3078_cov_4.393979, whole genome shotgun sequence.
ACCESSION   DAOSNB010000294 DAOSNB010000000
VERSION     DAOSNB010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31769_length_3078_cov_4.393979"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..538
                     /locus_tag="PLG42_10095"
                     /pseudo
     CDS             41..538
                     /locus_tag="PLG42_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843449.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            630..1244
                     /locus_tag="PLG42_10100"
     CDS             630..1244
                     /locus_tag="PLG42_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOU31600.1"
                     /translation="MKIICVGLNYKQHAAELNMSLPEKPMIFLKPDSALLKNNKPFFL
                     PDFSEDIHYEVELVVKIDRLGKGIAEKYAHRYFNEVTLGIDITARDIQTHLRKLNHPW
                     ELSKCFDGAAPVGKFLPVKSLKKDLNNLDFRLEINGRVVQQGNTSDMIFSIAEIIEYI
                     SVFFTLKTGDLIFTGTPPGVGPLHRNDHLVAYLEDVPVLDFMIK"
     gene            complement(1649..2485)
                     /locus_tag="PLG42_10105"
     CDS             complement(1649..2485)
                     /locus_tag="PLG42_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU31601.1"
                     /translation="MDIQRAFLNAEKNYQKNLEQFTAEVFSGIFLPSHGLEHHRRVWL
                     NAVEIASESDLSGVEDLYKFAGKLIIACYLHDTGMAYDRGERHGIKSVELCRRYLEKH
                     GLPGNEYEDMLEAIEKHDYKEVQAGSNNNLLGLILNTADDLDAFGFAGIYRYIEIYLE
                     RDIPLKILGEKILGNASGRFENFKKSYGSKAHLFNRHLERFRIVEDFCNNYIKESERY
                     RFGTGKPQGYCGIAEVISIMLQNHLNPVHGLDQSNITKNIDDSVIRWYFTELKKDSFH
                     IP"
     gene            complement(2545..2796)
                     /locus_tag="PLG42_10110"
     CDS             complement(2545..2796)
                     /locus_tag="PLG42_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOU31602.1"
                     /translation="MSRTLTFNELRSIKDRLPSGSIKKIADKLNLSEETVRNYFGGWN
                     FEKGESAGLHYEKGPNGGIVVIEDTTILDMAEKMIKTTK"
BASE COUNT          914 a          633 c          580 g          951 t
ORIGIN      
        1 agcattataa ctggcaagat cgcagcctgc gagaaaatca gccagttata atgccaacag
       61 gtttgatatc ccgttacttg cggaagagtt cctgcgagta aatattgatt ttgatttcag
      121 acgcaggaga tatgtggatg tccaggttat ttttcataaa aaggagcaga gaaccctgag
      181 tgcagcatat ctgttttact gcagaaagga acttgaagat gctcatagct caaaggctga
      241 tacgcaggca acatacgaga tcctgaaagc ccagcttgaa agatatcccg acctcgagaa
      301 tgatgttaag aaactggctg aattcagttc gtataacaat aatgcagatt ttgtgggaag
      361 aatcatctat gatgaacagg ggagggaggt ctttaatttc ggaaaatata agggaagact
      421 ggtagaagaa gtgctgaagg aagacccggc atattattcg tggatgatga acggcgaatt
      481 ctccctctat actaaaaagg ttctgacaga aataaaactc cgtcagttcg gaaaataaat
      541 ccttttgccc ggaaggaagg gtagaattag ggtttcaatg aagagttttt agtaataatg
      601 tttataatca aatatttgca tatttctata tgaaaattat ctgtgtggga ttgaattata
      661 aacagcatgc tgccgagctg aatatgagcc tgccggaaaa gccgatgatt tttctgaagc
      721 ctgattcggc actgttgaaa aacaacaaac ctttttttct tcccgacttt tcagaagata
      781 ttcactatga agttgagctg gtggtgaaga ttgacaggct gggcaagggc attgctgaaa
      841 agtatgcaca tcgctatttt aatgaggtga cactgggcat cgatataaca gcccgcgata
      901 ttcagaccca cctgcggaag ctgaatcatc cgtgggagct ttcaaaatgc tttgatggag
      961 ctgcaccggt aggaaagttt ctgccggtaa aaagcctgaa gaaggatcta aacaatctgg
     1021 atttcagact tgaaataaat ggcagggttg ttcagcaggg gaacacatcg gatatgatat
     1081 tcagcatagc agagataata gagtatatat cagtcttttt cactcttaaa accggggacc
     1141 tgatttttac aggcactcct cctggcgtcg gcccactgca tcgtaacgat caccttgttg
     1201 cttacctgga agatgtgccg gtgctggatt ttatgataaa ataaagataa ctactcttac
     1261 tttcattgct tgcctgaccc cctgagaagg gggcttgtgg ttcatgttga ccccccaacc
     1321 ccctggcagg gggctttaat atcattcatt tctaaattat cagctccgga ggaacggcat
     1381 gtttgtagca atggattttc ctcatgtact cacccccaac cccctctcta cttcgtaaag
     1441 agggggctat ctttccttat tatttaaatg aatacataat cccatagatc tatatgacca
     1501 gaacccctgc ggggtactgg ttatatgtaa attttgggac cgaattccta tatgcgaaaa
     1561 tagcttacct gaattatctt attgcccgta gggcataagg ttttgtaaga gataatgcca
     1621 gcaatgattg ttttttacct cgtagaggtt aaggtatatg aaatgaatct ttcttcagtt
     1681 ccgtgaaata ccatctgata actgaatcat ctatattttt agttatattg gattgatcca
     1741 gtccatgcac agggtttaaa tggttttgaa gcattatgct aataacctct gcaatgccgc
     1801 aatagccttg tggtttacct gttccgaaac gatatctttc tgattctttt atgtaattat
     1861 tgcagaaatc ctcaactatt ctgaaccttt ctagatgacg gttgaacaga tgcgccttac
     1921 tgccgtatga ctttttgaaa ttctcaaacc ttcccgatgc attgccaagg atcttttcgc
     1981 ctaaaatttt taacggtata tctctttcaa gatatatttc aatgtatctg taaatccctg
     2041 caaagccgaa tgcatccaga tcatctgcag tattcagaat aaggcccaat aaattgttgt
     2101 tgcttcctgc ttgtacttct ttgtaatcat gtttttcaat cgcttcaagc atgtcttcat
     2161 attcattccc gggaaggccg tgtttctcaa ggtaccttct gcacaattca acacttttta
     2221 taccgtgacg ctccccgcgg tcataagcca ttcctgtatc atgaaggtaa catgcaatta
     2281 tcagcttgcc agcaaacttg tacagatctt ccacccctga taaatcactc tctgaagcta
     2341 tctcaacagc atttaaccac acccttctgt gatgctccag tccgtgcgac ggcaaaaaaa
     2401 taccactgaa tacctctgca gtaaattgct ccaggttctt ctgataattt ttttcggcat
     2461 tgagaaatgc cctctgaata tccatccgta tagataaaaa ccgtacgtaa taatttacag
     2521 ggaaatcaaa aataagagga tctgctattt tgttgtcttt atcatctttt ctgccatgtc
     2581 gagaattgtc gtatcctcta tcaccacaat ccctccgttt ggaccctttt catagtgcaa
     2641 acccgcactc tcaccttttt caaaattcca gcctccaaaa taattcctta ctgtttcttc
     2701 actcaaatta agcttatcgg caattttttt aatgcttcca gaaggaagac gatccttaat
     2761 gcttcttaat tcattgaaag tgagtgttct gctcatgatt atccttcttt aaagttcaac
     2821 cactaacaat caaagataat aaaaatatac catagaaaaa attttacaat ttaaaaaatg
     2881 ttatgctatt aagatacaga aatacagatt gttataaaga aattaaagtt ctttaaccat
     2941 tcccataaac aacttcaatc cttccttctt tttctcgtca aaaatatagt caatattgtg
     3001 agttaaatat tttttcaaag tttcacctct cattggcgat gaattactga aatcctcaac
     3061 taccctgtca atattatc
//
LOCUS       DAOSNB010000295         3051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32157_length_3051_cov_2.841121, whole genome shotgun sequence.
ACCESSION   DAOSNB010000295 DAOSNB010000000
VERSION     DAOSNB010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32157_length_3051_cov_2.841121"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(351..1610)
                     /locus_tag="PLG42_10115"
     CDS             complement(351..1610)
                     /locus_tag="PLG42_10115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOU31603.1"
                     /translation="MATPVIMPKQGQSVETCVITTWFKHKGEYVKAGDLLCSYETDKA
                     SFDLESPAEGTILDIFFNDGDEVPVLTAIAVIGAEGEDIKPFIPGTTHRTTNEAVTTP
                     QIQEPLSSSPAETTTVATPEGKIKISPRARNLALKEKIDITKIKGTGPGGRIIIRDVE
                     NILKTRAQEPEPRAAQLLHAEPSYAEYTDKPLSNIRKIIAGAMHASLQNSAQLTHHMS
                     ADVRKILEARKKIKEEIQAGKKISDITLNDMICYCVIKALKKFPEVNSHFPGDRIRTF
                     TDVHLGLAVDTPRGLMVPAIRNAGSMSLQVLSGKLKEAAEACRRGSIDPELLKSTAAS
                     FTVTNLGAYGVEMFTPVLNLPQVAILGVCTIIHRPADLGNGVMGFIPVIGLSLTYDHR
                     AIDGGPATLFLKEIKEQIETFNLPTLL"
     gene            1749..2456
                     /locus_tag="PLG42_10120"
     CDS             1749..2456
                     /locus_tag="PLG42_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOU31604.1"
                     /translation="MESLPRYKELYRILKKHIQDGIYREGDLLPSENELCSIYGMTRP
                     TVRQALALLAADGLIRKHQGKGSIVHRMPREIGILSVSGTTSAVGNRGLKTDIIVKPS
                     IKPWPDNFMFPLSETEKESGCIYMERLRLLEGTPIFYDINYIANINLPRFTSRQFMDR
                     SLFKILRDHYGVEIKGGEQRIRAIPATDKIGRFLKLKKSSPVLHLERKMETNVNGLYV
                     YSSIYCNTEKYSIFGTF"
     gene            2620..>3051
                     /locus_tag="PLG42_10125"
     CDS             2620..>3051
                     /locus_tag="PLG42_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOU31605.1"
                     /translation="MPHGNIAIVFDCGATNVRVAALNEKGDIIASESYQNNTKPDPFY
                     PGWRIWDAEEIWEKMCLACRRVVAGIDKNDIAGVTVTTFGVDGTLFDKDGNMLYPIIS
                     WQCERTVPIMQNIGKYIPVEEIYSEAGVLPFNFNTINKLVWF"
BASE COUNT          787 a          604 c          672 g          988 t
ORIGIN      
        1 gaaatcggca tcggagtaat tctctctctc ttctgcaatg gttttgctgt actggtttac
       61 aggtattttt ccgaaagtga tgaaccctgg ttttctgacc tcctttggat cgatgttttg
      121 tgttcttggc atataaattt gctttatttc attttcctga ttttatcctt taattaaatt
      181 aattcaatct gtatttattc attactattt tccttcatat taattcttca gttcttgttt
      241 ctcatttcta ttcaaatata atctggttag cttcctgttt ctctcttttt tctttccctc
      301 ctgcaaaatg ttgagagaga aaatgttctt tgttacgata tgctatgatt ttacaacaag
      361 gttgggaggt taaatgtttc aatctgttct tttatctctt taaggaagag tgttgcaggg
      421 ccgccatcta ttgcccggtg gtcgtatgtg agtgatagcc cgattaccgg gatgaagccc
      481 atcacaccgt tgccaaggtc ggcagggcgg tggattattg tgcatacacc caggattgct
      541 acttgcggca ggttcaaaac tggtgtaaac atttccacgc cgtatgctcc aagattcgta
      601 acggtgaaag aggctgctgt gcttttgagc aattcggggt cgatacttcc ccggcggcag
      661 gcctctgctg cttcctttag ttttccggat aaaacctgca gactcatgct gccggcattc
      721 cttattgcag gaaccatgag tcctcgcggc gtgtcaacag ccaacccgag atgcacgtcc
      781 gtgaaggttc taattctatc acctggaaaa tgactgttga cttccgggaa ttttttcagg
      841 gcttttatga cacaataaca gatcatgtca ttcagtgtaa tatcactgat tttctttccg
      901 gcttgaatct cttccttaat tttttttctt gcttcaagaa tttttcttac atcggcgctc
      961 atatgatgag tgagctgtgc cgaattttga agagatgcgt gcattgcccc tgcaattatc
     1021 tttcttatat ttgaaagagg tttatcggtg tattctgcat aagatggttc ggcatgcagc
     1081 aactgtgcag cgcggggttc tggttcctga gcccttgttt tcagtatatt ttctacatcc
     1141 ctgatgatta ttctccctcc tggtcccgta ccttttattt ttgtgatatc tattttttct
     1201 ttcagggcaa ggtttctggc gcggggtgag atttttattt ttccttcagg cgttgcaacg
     1261 gttgttgttt cagccgggct tgaggataag ggttcctgaa tttgcggagt agttactgct
     1321 tcattagttg ttctgtgggt tgtaccgggt atgaatggct taatatcttc accttcggct
     1381 ccgataactg caatggcagt aagaaccggc acttcgtcgc cgtcgttgaa aaaaatatcg
     1441 agtattgttc cttcggcagg tgattcgaga tcgaatgaag ccttgtctgt ttcgtacgag
     1501 cagagcaggt ctcctgcttt cacatactcg cctttgtgct tgaaccatgt agttattaca
     1561 catgtttcga ccgactgtcc ctgttttggc attattaccg gtgttgccat tgttttattt
     1621 tttaacttgt taagatatgt ttaaaagttg tcttgttaaa ataagattat atggcatgtg
     1681 caaaaataat taatttaact tgtatttaca agtaaaaaat aatatattta cattttaaaa
     1741 ttcaggaggt ggaatcatta cccagatata aggaacttta caggatttta aagaagcata
     1801 tacaggatgg gatataccgg gagggtgacc tgctcccatc ggaaaatgag ttatgcagca
     1861 tatatggcat gacgcgacct acggtgaggc aagcacttgc tttgcttgcc gctgacggtc
     1921 tgatacgcaa gcatcagggc aaggggagta ttgttcaccg catgccacgt gagataggga
     1981 tcctttcggt ttcgggaaca acatcagcag taggcaaccg gggactgaaa acggacataa
     2041 ttgtcaagcc ttcaataaaa ccctggccgg ataacttcat gtttccactg tcggagacag
     2101 aaaaggaatc gggatgtatc tacatggagc gtctgaggct gcttgaaggc acaccaatat
     2161 tttatgatat taattatata gcaaatatta atttgccacg ttttacttca cgccagttta
     2221 tggacagaag cctgtttaaa atcctgcgcg atcattatgg cgttgagata aaaggtggcg
     2281 agcagcgcat cagggcaata ccggcgacgg ataaaatagg cagatttctg aaactgaaaa
     2341 aaagctcacc cgttctgcat cttgaaagaa agatggagac aaatgtgaac gggctttatg
     2401 tttattcttc aatatattgt aatacagaaa aatattcgat attcgggacg ttctgaatct
     2461 gaaatggctt tcaaaattta ttcataaaga tatcagatag tttatgtaca aattgacttt
     2521 tatggctgaa tagtaatatt gcattgtact tctgatttaa ttaaccggca catcaatttt
     2581 tgcgaatcat tctttatctt tgattgcaaa gtttttaata tgcctcacgg aaacattgca
     2641 atagtcttcg actgcggcgc aaccaatgtg cgagttgccg cattgaacga gaaaggagat
     2701 ataatagctt cggaatcgta ccagaacaat accaaacccg accccttcta tcctggctgg
     2761 cgcatctggg acgccgaaga aatatgggaa aagatgtgcc tggcatgccg cagggtggtt
     2821 gccgggatag ataaaaacga catcgctggc gttacagtaa caaccttcgg ggtggacggt
     2881 acactattcg acaaagacgg caatatgctt tatcctatca tttcgtggca gtgtgaacga
     2941 actgtgccca taatgcagaa tataggtaaa tacatcccgg ttgaggaaat ctattcagaa
     3001 gccggtgtac tgccttttaa ctttaatacc atcaataaac ttgtatggtt t
//
LOCUS       DAOSNB010000296         3038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32348_length_3038_cov_4.232652, whole genome shotgun sequence.
ACCESSION   DAOSNB010000296 DAOSNB010000000
VERSION     DAOSNB010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32348_length_3038_cov_4.232652"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="PLG42_10130"
     CDS             <1..1461
                     /locus_tag="PLG42_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU31606.1"
                     /translation="DQTANRKVANNFSGFKNLRLFFCNGKDVFDSMNVMSEAKEYVKS
                     TGNPAIVHANCVRIHSHSNSDRHELYRDENERNYVAEYDPLAKFKRLLLRYKRFTAEE
                     LEDIESETKEIIKQAHREAMSAPDPEPSSVFNFVIPEPYPASKYPDGTHDFKGEPKKL
                     IEALNETLKAEFRHNPDTYIWGQDIANKEKGGIFNVTKGMQKEFGKERVFNAPIAEDF
                     IVGTANGMSRFSDRIRIVIEGAEFADYFWPAMEQLVDTSHDYWRSNGKFSPNITIRLA
                     SGGYIGGGLYHSQTIEGALTTFPGIRVVYPSFADDAAGLLRTSIRSRGTTVFLEPKAL
                     YNDIRAATPVPDDFEVPFGKARIRMEGDDIGMISYGNTTIFCLEAAERLRKEHGFTTR
                     VLDLRSLNPLDEEAIMETVKKTGKILVVHEDKVFGGFGGEIAALISEKAFEYLDGPVV
                     RVGSLFTPVGFNKILEKTILPDTDKIYRAAIDLLKY"
     gene            1476..1982
                     /locus_tag="PLG42_10135"
     CDS             1476..1982
                     /locus_tag="PLG42_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOU31607.1"
                     /translation="MEKTAIIYSFNTAKTAQAARLIYENFSDKNIEMINAEDITGEKF
                     LQYKNLILGVPTWFDGELPNYWDEFVPELEELDLNGLRVAIFGPGDQKNYPENFVDGI
                     GILGRLLEERGAELVGFVPADEYNFEKSLALRGDKFMGLPLDFENQASKNKERIIRWV
                     NQLKNELY"
     gene            complement(1992..>3038)
                     /locus_tag="PLG42_10140"
     CDS             complement(1992..>3038)
                     /locus_tag="PLG42_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOU31608.1"
                     /translation="TCWTCKSSNVPGIIEEEGAAGFYRGTWEKRGKQIINPIGCADCH
                     DSKTMNLRISRQALLEAYKAAGKDIGKVSFQEMRSLVCAQCHVEYYFNKNIVEGVDYL
                     VFPWKNGLTAEDIERYYDELRFTDWTHALSRAPMLKAQHPDYEVYMTGTHASRGVSCA
                     DCHMPFISEGGQKFTDHHIQSPLNNVANSCQVCHREETERLVNDVYLRQDKIMESRKI
                     LEGLLVKCHFEAKAAWDKGAKEDQMKEILNDIRRAQWRWDFATAGHGNSFHSPLETLR
                     LITSGISIASEARLKLSKVLVSLGYTGEVKVPDISEKAKAQRLIGLDIEKLTEEKSEF
                     LKKVVPVWNKSEAN"
BASE COUNT          895 a          656 c          621 g          866 t
ORIGIN      
        1 gaccagactg ccaaccgtaa agtggcaaac aatttttccg gttttaaaaa cctccgcctc
       61 ttcttctgca acgggaaaga cgtctttgat tcaatgaatg tgatgtccga agcaaaagaa
      121 tatgtgaaat ccaccggtaa ccctgctatc gttcatgcca actgcgtacg cattcattca
      181 cactccaatt ctgatcggca tgaattgtac cgtgatgaaa acgaaagaaa ttatgttgcc
      241 gaatatgacc ctctcgcaaa attcaagcgt cttcttctga gatataaaag attcacagct
      301 gaagagctgg aagatataga aagtgagaca aaagagatta tcaaacaggc acacagggaa
      361 gccatgagtg ctcctgaccc tgaaccatca tccgtattca attttgtcat tcccgagccc
      421 tatcccgcat ccaaatatcc cgacggaaca catgacttca agggagaacc aaaaaaactt
      481 atcgaagcac tcaatgaaac tcttaaggcg gaatttcgtc ataatccgga tacttatatc
      541 tgggggcagg acatagcaaa caaggaaaaa ggagggatat ttaatgtgac aaaagggatg
      601 cagaaagagt ttggtaaaga aagggttttc aatgctccca ttgctgaaga tttcattgtt
      661 gggacggcta acggtatgag ccgtttcagc gacaggataa gaatagtaat tgaaggtgct
      721 gagtttgccg actatttctg gcctgctatg gaacagcttg tagatacttc tcatgattac
      781 tggagatcaa atggtaaatt ttcaccaaac ataacaatcc gtcttgcatc aggaggttac
      841 attggaggag gactttacca ctcgcaaacc attgaaggag cattgacaac ttttccagga
      901 attcgtgtgg tatatccgtc atttgctgac gatgcagcag ggctgttgcg cacatcaatt
      961 aggtcgagag gcacaacggt ttttcttgaa ccgaaagctc tttacaacga catccgggcc
     1021 gctactcctg tacccgatga ttttgaagta ccgttcggca aggctagaat cagaatggaa
     1081 ggtgatgata ttggaatgat atcatacggc aacacaacaa tattctgcct cgaagcagct
     1141 gaacgactca gaaaggaaca tggctttaca acgcgtgtcc tcgatctgag atctcttaat
     1201 ccacttgacg aagaggctat catggaaaca gtaaagaaaa cagggaaaat tcttgtagtc
     1261 catgaagata aagtatttgg tggtttcggt ggagaaatag ccgcattgat atcagaaaag
     1321 gcatttgaat acctcgatgg tcctgttgta agagtgggct cactctttac acctgttgga
     1381 ttcaacaaaa tacttgaaaa aaccatttta ccagatactg ataaaatata ccgggcagct
     1441 attgatttgt taaaatatta atttctcaat aaactatgga aaagactgct atcatataca
     1501 gttttaacac ggcaaaaacc gcacaggcag ccagactcat ttatgaaaat ttttcagata
     1561 aaaatattga aatgataaat gccgaagata ttaccggaga gaaatttctt cagtacaaaa
     1621 atctgatcct cggtgttcct acatggttcg atggagaact tccgaattac tgggatgagt
     1681 ttgttccgga acttgaggaa cttgatttga atggactcag agttgcaata ttcggaccgg
     1741 gcgaccagaa aaactatcct gagaactttg ttgacggtat tggtattctt ggccgacttc
     1801 tggaagaaag gggtgctgaa ctggtaggtt ttgttcctgc agatgaatat aacttcgaaa
     1861 aatcacttgc actcagaggt gataaattta tgggactacc tctcgatttt gaaaatcagg
     1921 cttcaaaaaa caaagagagg attatcaggt gggttaacca gttaaaaaat gagctttatt
     1981 gagctattta cttagtttgc ttctgatttg ttccagaccg gcacaacctt tttcaggaat
     2041 tccgattttt cttctgttaa tttttcaata tccaatccaa taagcctctg tgcttttgct
     2101 ttttcagaaa tatccggcac ttttacttcc ccggtatatc ccagagaaac aagtaccttt
     2161 gatagtttca gcctggcctc agaagcaatt gatattccgc ttgttattaa ccttaatgtc
     2221 tcaagtgggg agtgaaaaga attgccatga cctgccgttg caaaatccca gcgccattgt
     2281 gctcgtctaa tatcattgag aatctctttc atctgatctt cctttgcacc tttatcccag
     2341 gctgcctttg cctcaaaatg acatttaacc agaaggcctt ccagtatttt gcggctttcc
     2401 attattttgt cctgccgcag ataaacatca ttcaccagcc gttcggtttc ctccctgtga
     2461 caaacctgac atgaatttgc aacattgttg agcgggctct gtatatgatg gtcagtaaat
     2521 ttctgccctc cctcacttat aaaaggcatg tggcagtcgg cacaggatac tcctcttgag
     2581 gcatgggtgc cagtcatata aacctcataa tcgggatgct gtgctttaag catcggggca
     2641 cgacttaaag catgtgtcca atctgtaaaa cgaagctcat cgtaatatcg ttcaatatct
     2701 tcggcagtca atccgttttt ccaaggaaag acaaggtaat caaccccctc aacaatattc
     2761 ttattgaaat agtactctac atggcactgt gcgcatacca gactcctcat ttcctgaaaa
     2821 gataccttgc cgatatcctt tcctgctgct ttatatgctt ccagcagtgc ctgccttgaa
     2881 attctcagat tcatggtttt tgaatcatga caatcggcac atcctattgg atttataatc
     2941 tgtttccccc tcttttccca tgttcccctg tagaaaccgg cagctccttc ctcttcaata
     3001 atccctggta cgttcgagct tttgcatgtc cagcatgt
//
LOCUS       DAOSNB010000297         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32575_length_3021_cov_3.354349, whole genome shotgun sequence.
ACCESSION   DAOSNB010000297 DAOSNB010000000
VERSION     DAOSNB010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32575_length_3021_cov_3.354349"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..361
                     /locus_tag="PLG42_10145"
     CDS             86..361
                     /locus_tag="PLG42_10145"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOU31609.1"
                     /translation="MADRYYPLKIEREDEEKIRKAARMINEKVLQYKQRYSDKDVQDF
                     LAMAALQFVIKLIEEEEKHNNDFLPEALNELANKIDTVLEKKSNDVL"
     gene            611..2173
                     /gene="rny"
                     /locus_tag="PLG42_10150"
     CDS             611..2173
                     /gene="rny"
                     /locus_tag="PLG42_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOU31610.1"
                     /translation="MTAIATNIFNGLTIVIASFCLVAGFGISYLLWHTLLKKRKEKII
                     AEAESEAEVIKKEKILQAKELFLQMKSEHEKVITEKNNKIIQAENRLKQKELTLNQKI
                     EEVQRKKNEVDAIRDNLTAQLELIEKKSEELDKLHRQQIEKLEIISGLSAEEAKNQLI
                     ESLKEEARTEAMSYINEIIDEAKMTANKEAKRIVIQTIQRVATEHAVENAVTVFHIDS
                     DEMKGRIIGREGRNIRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLALHQLVTD
                     GRIHPARIEEIVEKTRKQVEEEILEVGKRTAIDLGIHGLHPELIRLIGKMKYRSSYGQ
                     NLLMHSREVANLSAIMAAELGLNPKLAKRAGLLHDIGKVPDDEPELPHAILGMRLAEK
                     YKEKPEVCNAIGAHHEEVEMTTLIAPIVQVCDAISGARPGARREVVESYIKRLKELES
                     LALQYPGVTKTYAIQAGRELRVIVGADKTTDKDVENLSYEIARRIQSDMTYPGQIKIT
                     VIRETRAINYAK"
     gene            2318..>3021
                     /locus_tag="PLG42_10155"
     CDS             2318..>3021
                     /locus_tag="PLG42_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOU31611.1"
                     /translation="MNKIKIISLVGARPQIIKASAISRAIKNKFSSKIKEVIVHTGQH
                     YDRELSQVFFEELEIPKPDYNLGVGSARHGKQTALMLTGIEEVLLTERPDCLVIYGDT
                     NSTLAGALAAVKQHFPVVHVEAGLRSFNKLMPEEINRIMSDHASTLLFAPTNAAFKNL
                     VAEGFRPENSPPYSINNPKIYLTGDIMYDNSFFFSSLAEKKKSYMLEKLNIERDGFVL
                     VTIHRDINTDDILRLNT"
BASE COUNT         1024 a          534 c          677 g          786 t
ORIGIN      
        1 gtgcgggaaa tagataaatg tattgctctt ttagacaaat gataatatta caattgatga
       61 taataaactt tcaatcaggg ttaatgtggc agatagatat tatccattga aaattgaaag
      121 ggaagatgag gagaaaatca gaaaggctgc caggatgatt aatgagaagg tcctgcaata
      181 taagcaaagg tattctgata aagatgtaca ggattttctg gcgatggcag ccctgcagtt
      241 tgtgataaag cttattgaag aagaagaaaa acacaataat gattttctcc ccgaagcgct
      301 gaatgaactg gcaaataaaa ttgatacagt tctggagaaa aagagtaatg acgttcttta
      361 aaataaaaat atttatctaa tacccgcatt gtttcttcaa taatttttgg aaactcaaca
      421 ctaatcactt tggggtcaag ctcagtttat gcaggacagg cctcatcccg accaggtcgg
      481 gatcccgata aatatcggga cagtggaagt tcgagataag ctggcagccc catccataca
      541 gaaatggggt tttataaaaa tttgaggaaa ctttgcgggt ttttatttat taacttaaaa
      601 tgttatagtt atgacggcaa ttgcaacaaa tatttttaac gggcttacta tagttatagc
      661 ctcgttctgc cttgtggcag gatttggaat ttcttatctg ctgtggcata cacttttaaa
      721 gaaaagaaaa gaaaaaatca ttgcagaggc agaatcagaa gcagaagtaa tcaagaagga
      781 aaaaatattg caggctaagg agctcttcct tcagatgaaa tctgagcatg aaaaggttat
      841 tacagaaaag aataataaaa taattcaggc tgaaaacaga ctcaaacaga aggaacttac
      901 tcttaatcag aagattgaag aagttcagcg taaaaagaat gaggtagatg caataagaga
      961 taatctcact gcacagcttg agctgattga gaaaaaatca gaagaacttg acaagcttca
     1021 ccggcagcaa attgagaaac ttgagattat atcgggtttg tcagctgagg aggccaagaa
     1081 tcagctgatt gagtcattga aggaagaggc gcggaccgag gcaatgtcat atatcaatga
     1141 gattattgat gaggctaaaa tgacagccaa taaggaggca aagaggattg ttatccagac
     1201 tattcagagg gttgcaacgg aacatgctgt tgaaaatgca gtcactgtat ttcatatcga
     1261 ttccgatgaa atgaaaggaa ggataatagg gagggaaggc cgtaacatca gggcacttga
     1321 ggctgctacg ggtgttgaga taattgttga tgatacgcct gaggcaattg ttctatcagc
     1381 ttttgaccct gttcgccgtg aaatagcacg tcttgcgctc catcagctcg taaccgatgg
     1441 tagaatccat cctgcaagga ttgaggaaat agtggaaaag acccgcaagc aggtggagga
     1501 ggagatactt gaggtaggaa agagaacagc tatcgacctt ggtattcatg gcctgcatcc
     1561 tgaacttatc agactcattg gcaaaatgaa atacaggtca tcctatggtc agaacctgct
     1621 tatgcattca agagaagttg caaacctcag tgccatcatg gctgctgaac tcggactcaa
     1681 tccaaaactt gcaaagagag ccggtctgct gcacgatata ggaaaagttc ccgatgatga
     1741 gccggagctg ccacatgcca ttcttggtat gcgtctcgca gaaaaatata aggaaaaacc
     1801 tgaagtatgt aatgctatcg gagcccatca tgaagaggtt gagatgacaa cactgatcgc
     1861 tcctatagta caggtatgtg atgcgatttc tggagcacgc ccgggagctc ggagagaagt
     1921 ggtggaatcg tacatcaagc gactcaaaga acttgaatca cttgcacttc agtatcctgg
     1981 tgttacaaaa acatacgcaa tacaggcagg gagagaactg agagttattg tcggtgcaga
     2041 taaaacaacc gataaagacg tagaaaacct ctcatatgag attgcccgca ggatacaatc
     2101 cgatatgaca tatccgggac agataaaaat tacagttata agggaaacaa gggcaattaa
     2161 ctatgccaaa taatcagaat tgacttcaaa acaccttgat tcgaaaagct ctgtttgttg
     2221 aatgaagtat tgtatctgct gattggatag tgctattttt attgtttgaa ttatttataa
     2281 agtctaaatt tgggtttgct tttaaagaaa tttattaatg aacaaaataa aaataatcag
     2341 tcttgtagga gccaggcccc agattattaa agcttcagca atcagcaggg caatcaaaaa
     2401 taagttttcg tcaaagatta aggaggttat tgttcatacc ggccagcatt acgaccgtga
     2461 gctttctcag gtttttttcg aagaactcga aattccaaaa cccgattata atcttggtgt
     2521 tgggtcagcc cgccacggaa aacaaacagc gctgatgctg accggcattg aagaagtgtt
     2581 attgaccgaa cggccagatt gtcttgtaat atatggcgac acaaactcaa cacttgcagg
     2641 agctcttgca gcagtaaagc agcactttcc ggtggtacat gtagaagccg gcctacgctc
     2701 atttaataaa ttgatgcctg aagagataaa tcgcataatg agtgaccatg catcaacact
     2761 tctttttgca cccacaaatg ctgcattcaa aaatcttgta gctgaaggtt tccgacccga
     2821 aaacagtcca ccttacagta ttaacaaccc gaaaatttac cttacaggtg atataatgta
     2881 tgataattcc tttttctttt cctcgcttgc agaaaagaaa aagtcataca tgcttgagaa
     2941 actgaatatt gaaagggatg gatttgttct tgttaccatt cacagggata tcaacactga
     3001 tgatatactg aggcttaata c
//
LOCUS       DAOSNB010000298         3013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32691_length_3013_cov_4.744760, whole genome shotgun sequence.
ACCESSION   DAOSNB010000298 DAOSNB010000000
VERSION     DAOSNB010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32691_length_3013_cov_4.744760"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..342)
                     /locus_tag="PLG42_10160"
     CDS             complement(1..342)
                     /locus_tag="PLG42_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31612.1"
                     /translation="MRPLFITSIFFTALFLITGSSVERLRPADRKATRETVNLFRALY
                     RLQQRGVMYGHQDDLMYGYTWWYEKGRSDTRDFTGDYPAVAGFELGHLELGDERSIDS
                     VLLLIIFYATD"
     gene            457..729
                     /locus_tag="PLG42_10165"
     CDS             457..729
                     /locus_tag="PLG42_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOU31613.1"
                     /translation="MGEIVLYKIQVTGYVQGVGFRYCTVREARRLGIKGYVRNMPDGS
                     VYIEAEGDREQLEALVRWCNEGPGYVESVKVSTYPPVGYKDFSIKY"
     gene            complement(732..1493)
                     /locus_tag="PLG42_10170"
     CDS             complement(732..1493)
                     /locus_tag="PLG42_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOU31614.1"
                     /translation="MTFAGENTPGSVHIGTSGWSYAHWAGVFYPGDIKPAKYLEYYTT
                     RFSCVELNSSFYHLPSESTVKGWLNRTPASFIFCPKFSRYVTHELHLMNADEATERFF
                     EVFRELKPKLGPVLIQLPPSLKYDEPLVSTFLGILKEKYSSFRFAVEIRNKSFLSDKF
                     FNLLNSYNMAFVIADSGNRYPYSEVVTAGFIYIRFHGRENLYASDYNDDILREYAEKI
                     KKWNHEGKEVWVFFNNDFHGYAVKNALSLMKMLNS"
     gene            1602..2099
                     /locus_tag="PLG42_10175"
     CDS             1602..2099
                     /locus_tag="PLG42_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOU31615.1"
                     /translation="MIAAVDVYYREDSGAVAAAVVFSSFTDNEPYAVYISEIQNAENY
                     ISGQFYKRELPCIITVFKKIKEKIDILIIDGYIDPGNKPGLGRHLWRTLGEKVIVIGV
                     AKTFFKGTEAVKVFRGRSRRPLYITSAGIDTFEAASLIEKMPGNFRIPDMLKLVDQIS
                     RTGIW"
     gene            2231..>3013
                     /locus_tag="PLG42_10180"
     CDS             2231..>3013
                     /locus_tag="PLG42_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOU31616.1"
                     /translation="MIISASCEKDSTQGKYPKLLVGDTEISISEDEPNGVAEIPMYLS
                     IVPKTGGSFEYYTADSTAKAGKDYKSISREKISFKPGETYVIIRVEIIPNSEKIYDAA
                     FKIKLENAENCVLPGSELVIKILNTDYETLVWSDEFNGGSLSTVFWNYETGGGGWGNN
                     ELQTYTNSNENVHLDSGYLHITALNPSPGSYTSGRITTKGKKEFTNGKVEIRAMLPEG
                     KGLWPALWMLGANFSSVGWPTCGEIDIMESLGHAPSTSYGALH"
BASE COUNT          935 a          523 c          661 g          894 t
ORIGIN      
        1 tcagtccgtt gcgtaaaaga taattaacag gagaaccgaa tcaatacttc tttcatcacc
       61 cagttctaaa tgtccaagct caaaacctgc aacagccgga taatcacccg tgaaatcacg
      121 agtgtcagat ctgccttttt cataccacca ggtatagcca tacatgaggt cgtcctgatg
      181 cccgtacatt actcctctct gctgaagtct gtaaagagct ctgaaaaggt taacagtttc
      241 acgggtggcc tttctgtcgg caggccttaa tctttcaacc gaggagccag taatcagaaa
      301 cagtgctgta aaaaagattg atgttataaa tagaggtctc attattaatt ttttagtttg
      361 atatcaaaat tacaaaaaca aacctggatt ctgaacgaaa aaaaagattt cttaatttta
      421 tgttctacca aaaagccttg catcaaagga aataaaatgg gagaaatagt tctttataaa
      481 atacaggtta ccggttatgt tcagggagta ggattcagat actgtactgt aagggaagcc
      541 agacgactgg gaataaaagg atatgtgcgt aacatgcctg atgggagtgt gtacattgag
      601 gctgaaggag acagggaaca gctggaggct ctcgtgagat ggtgcaacga aggccctggt
      661 tatgtggagt cggttaaagt aagtacatac cctccggttg gctataaaga cttttcaatc
      721 aagtactgat ctcaggaatt taacattttc atcaggctta aagcgttttt cacagcgtaa
      781 ccatgaaaat cattgttaaa aaatacccat acttctttac cttcatgatt ccattttttt
      841 atcttttcgg catactcacg gagaatgtcg tcgttgtaat ctgaagcgta gaggttttcc
      901 cggccgtgga acctgatgta aataaaccct gctgtaacaa cttcactgta aggatacctg
      961 tttcctgagt cagcaattac aaatgccatg ttgtagctgt tcaaaaggtt gaaaaatttg
     1021 tcagataaga aggatttgtt tcttatttcc actgcaaacc ggaatgaaga atatttttcc
     1081 tttagtatgc ccagaaacgt tgaaaccagg ggttcatcat atttcaggga ggggggtagc
     1141 tgaatgagaa ccgggcccag tttgggttta agttctctga acacttcaaa aaatctttca
     1201 gttgcttcgt ctgcattcat aaggtgcaat tcatgcgtaa catacctgct gaattttggg
     1261 cagaaaataa atgaagctgg tgtacggttg agccagcctt ttacggttga ttccgacgga
     1321 agatgataga aacttgaatt cagctcaaca caactgaatc gggtggtgta gtattcaaga
     1381 tattttgccg gcttaatatc tccgggataa aatacccctg cccagtgtgc gtagctccat
     1441 cctgatgtac ctatatggac acttcccggc gtgttttctc ctgcaaatgt catattgaga
     1501 aaatgtatat tttaaagtaa atttagttca cttttatcaa agtatttttc ttacgaaaat
     1561 aattttgttt caggatgaag cattaaatat ataattcagt aatgatagct gctgttgacg
     1621 tatattacag agaggattca ggtgctgttg ctgcagctgt ggttttttct tcttttactg
     1681 ataatgaacc ttatgcggtt tatataagtg aaattcagaa tgcggagaac tatatttcag
     1741 ggcagtttta taagcgtgaa ctgccctgta tcattactgt ttttaaaaag ataaaggaga
     1801 aaattgatat tttgattatt gacgggtata ttgatcccgg taataagcct gggctagggc
     1861 gtcatttatg gagaacactc ggtgagaaag taattgttat tggagtggct aagacatttt
     1921 tcaaaggaac agaagctgtg aaggttttca gagggagaag tcggagaccg ctttacatta
     1981 cttctgccgg aattgatacc tttgaagcag cctctctcat agaaaaaatg ccgggaaatt
     2041 tcagaattcc cgacatgcta aagcttgtcg accaaattag ccggacagga atatggtgaa
     2101 ttacccagga gaaataattt ggcataattt gattactttt gagaaacgtt aatttatagt
     2161 taagatgatt attttcaagt ttatttggga tttcagcagg gcgtattttt acgttttttg
     2221 cgttttatta atgattattt cggcatcatg tgagaaagat tcgacacagg gtaaatatcc
     2281 aaagcttctc gtaggcgata cagaaatatc tatatcggaa gatgaaccga atggtgtggc
     2341 agaaattccg atgtatcttt caattgtacc aaaaacagga ggaagtttcg agtattatac
     2401 tgctgacagt acagctaagg caggaaagga ttacaaaagc atttcaaggg agaaaatttc
     2461 atttaaacct ggcgaaacgt acgtgattat aagagttgag atcattccta acagtgagaa
     2521 gatttacgat gcagctttta aaatcaaact tgaaaatgcc gagaattgtg tcttacctgg
     2581 cagtgaactg gtaataaaaa tattgaatac tgattacgaa acccttgtat ggagtgatga
     2641 gtttaatggt ggttcactga gtacagtttt ctggaattat gaaaccggag gagggggatg
     2701 gggcaataat gagttgcaaa catatacaaa ttctaatgaa aatgttcatc tcgacagcgg
     2761 atatcttcat ataacagcac ttaatccttc cccgggctca tacacttcgg gaagaataac
     2821 aacaaaaggc aaaaaagaat tcaccaatgg aaaagttgaa ataagagcca tgttgccgga
     2881 aggtaaaggg ttgtggccag ctttatggat gctgggggcc aatttttcaa gtgttggctg
     2941 gcccacctgc ggtgaaatag acataatgga atctcttggc catgcacctt ctacatctta
     3001 tggtgcactc cac
//
LOCUS       DAOSNB010000299         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32823_length_3003_cov_3.964043, whole genome shotgun sequence.
ACCESSION   DAOSNB010000299 DAOSNB010000000
VERSION     DAOSNB010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32823_length_3003_cov_3.964043"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLG42_10185"
     CDS             <1..621
                     /locus_tag="PLG42_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOU31617.1"
                     /translation="LMADSGKQDFVLNSPEIREALIAKMKEAVELAKRTGAKMALVVP
                     GRYDMKLHMDYQTANVIDNLRYCCDVVEPEGITLVLEPLNPYINHPGLFLQRIPQAYA
                     ICRAVNRPSCKIVNDIYHQQITEGNLIPNIDMAWSEIAAFHLGDSPGRNEPTTGEINY
                     KNIFKHLYEKQYNGVLCMEHGISQRGTKEGELRVIQAYRECDNFEV"
     gene            722..1837
                     /locus_tag="PLG42_10190"
     CDS             722..1837
                     /locus_tag="PLG42_10190"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOU31618.1"
                     /translation="MKNYLFLFILSGLILFSTSCKKKELLYAGRFTKGDEKGLYIFSF
                     NPSNGSLKLKSQEDVGPSPSFFCFGRNNDLLYVINEVMEFKGAFGGGLTTLKLDTETG
                     RPVEKLSEILVPYAGPCHISLSADGRHILIANYPNGSVVVIRLGSNGIPEAVTDTILY
                     VKEEPDRSHAHMIKHDPAGKYIYVTDLGLDRIVTYTLDTINSKLVQTSDGIASLTHGS
                     GPRHFVFNNDGTIMYVINELGSTITVFSVDRQSGALTALQTVKTISDDYIENNYCAEI
                     ALSKNGKFLYGSNRGENTIIVFKVGEGGKLELTGRVSCGGDWPRNFVIHPEGKFMLVG
                     NQKSGYISVFKINQMTGLPEGPVAKAEIPGVAFLAFK"
     gene            1889..>3003
                     /locus_tag="PLG42_10195"
     CDS             1889..>3003
                     /locus_tag="PLG42_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOU31619.1"
                     /translation="MKLTIKFSAALLLILIFLTLSVSFTGDQRKPHSSYVVLVSLDAF
                     RWDYDSIYTTPNLKKIAEEGVMAKRLVSSFPTNTFPNHYSIATGLYPAHHGIVNNTFF
                     APDLGRLYRIGDRSAVENPDFYYGEPIWNTAQKQGALAASFYWVGSEAPVGGIHPYYW
                     KKYDESISYNARIDTVVKWLSYPPAKRPELVTLYFDEPDATGHDYGPVSPETGEVVSL
                     LDSLLGTLREKLSSLPHRDKINLIVLSDHGMTEVSSDRYINLCDIVPARMIKNTYGGN
                     PVVFIEPAEGKKDSVLLLINRTKGVKAWAKETLPERWNYGKSERISAIVAVADSSWYL
                     ATRPLTGKFRGGAHGYDNRNPDMYGIFYAAGPAFKRGV"
BASE COUNT          897 a          594 c          669 g          843 t
ORIGIN      
        1 ctgatggccg actcgggaaa gcaggatttc gttctcaact cgcctgaaat ccgtgaggca
       61 ctaatcgcaa aaatgaagga ggcagtggag ctggcaaaac ggacaggcgc caaaatggct
      121 cttgtggtac cgggcaggta tgatatgaaa cttcatatgg attatcagac agccaatgtg
      181 attgataacc tccggtattg ctgcgatgta gttgaaccag aaggaattac tcttgtgcta
      241 gaacccctga atccttacat aaatcacccc ggcctcttcc ttcagagaat tccacaggct
      301 tatgcaattt gcagagccgt taacagacca tcatgcaaaa tagtcaatga tatttatcat
      361 cagcagatta ctgaagggaa tcttattccc aatatcgata tggcatggag tgagattgca
      421 gcctttcacc ttggcgactc accgggccgc aatgaaccga caacagggga aataaactat
      481 aagaatatct tcaaacatct gtatgagaag cagtataacg gagttctctg catggaacat
      541 ggtataagcc agaggggcac aaaggaaggg gagctgagag ttattcaggc ataccgagaa
      601 tgcgacaact ttgaagtttg aattttttaa acagtctagt gaaggccgga ttttcccggc
      661 cttttttatt ttctttgcca aaaacttatt tttacaaatt caagatttaa aaaagagaaa
      721 aatgaagaac tatctgtttc tttttatcct gtcaggctta atactttttt cgaccagttg
      781 caagaagaaa gagctccttt atgctggaag atttacaaag ggtgacgaga aaggattata
      841 tatctttagt tttaatcctt ccaatggttc attgaaactt aaatcgcagg aagatgttgg
      901 cccgagccca tcatttttct gttttgggcg caataacgac ctgctctatg ttattaatga
      961 ggtaatggaa ttcaaaggtg cctttggggg cggactaaca acactgaagc tcgatactga
     1021 aacaggccgg ccagtggaga agctcagcga gattctcgtt ccttatgcag gcccctgtca
     1081 tatatccctc tcagcagatg gaaggcatat acttatagca aattatccaa atggttcagt
     1141 tgttgtgatc cgtctaggca gtaatggtat acccgaagct gttacagata caattctgta
     1201 cgtaaaagag gagcctgacc gttcccatgc ccatatgata aaacatgacc ctgctggtaa
     1261 atatatttat gtaactgacc tcgggctcga cagaattgta acctatacac ttgataccat
     1321 aaatagcaaa ctggttcaga cttctgatgg aatagcgtct ttgacacatg gttcaggtcc
     1381 ccgtcatttt gtgttcaaca acgatggcac tataatgtac gtcataaatg aactcggttc
     1441 aacaataaca gtcttcagtg tcgacaggca atcaggagca cttaccgctt tgcaaacagt
     1501 gaaaacaatc tctgacgatt atatcgaaaa taattactgt gccgagattg ctctgagtaa
     1561 aaacggaaag ttcctttatg gttccaacag gggtgaaaac acaataatcg tttttaaggt
     1621 cggagagggt ggtaagcttg aacttaccgg acgtgtttcg tgtggtggcg actggcctag
     1681 aaattttgtt attcatccgg aaggaaagtt catgcttgtc ggaaaccaga aatcaggata
     1741 tatttcagta ttcaaaataa atcaaatgac aggtttaccg gaaggacctg tcgcaaaggc
     1801 tgaaataccc ggcgtggcat ttcttgcatt taaataggtt ataattgact acttatcatt
     1861 aattactttt tagatgaata aatcttttat gaaactcact attaaatttt ctgctgcact
     1921 gttgctgata ttgattttcc tcactctttc agtttcattc acgggtgacc agcgtaaacc
     1981 tcacagcagt tatgtggttc ttgtatcgct tgatgctttc aggtgggatt atgacagtat
     2041 atatacaacg ccaaacctga aaaaaattgc tgaagagggt gtaatggcaa aaagacttgt
     2101 ttcctcattt ccaacaaata catttccaaa tcattattca attgccaccg gtctttaccc
     2161 ggcccatcat ggtatagtta acaacacatt ttttgcacct gatctggggc gtctataccg
     2221 tataggtgac agatcggctg tagagaaccc ggatttttat tatggagaac ccatatggaa
     2281 tacagctcag aaacagggtg ctcttgcggc ttcattttac tgggttggct cagaagctcc
     2341 agttggaggg attcatcctt actactggaa gaaatatgac gaaagtatta gttataatgc
     2401 aagaattgat actgttgtaa aatggctgag ttatccgcct gcaaaacgcc ctgagcttgt
     2461 aaccctttac tttgacgaac ctgatgctac aggtcacgat tacggtcctg tttctcctga
     2521 aacaggtgaa gttgtaagcc tgctcgactc tcttctgggt acgctgcggg aaaagctttc
     2581 ttcacttccg cacagggata aaataaacct tattgtgctt tctgatcatg ggatgactga
     2641 ggtatcatcc gacaggtata tcaatctttg cgatattgta cctgcacgca tgataaaaaa
     2701 cacttatgga ggtaatccgg tagtttttat cgaaccggct gaaggaaaga aagacagcgt
     2761 tttgttattg attaacagaa caaaaggagt taaagcatgg gcaaaagaaa ccctgcctga
     2821 gaggtggaat tacgggaaaa gtgaacgtat cagtgcaata gtggcagtgg ccgacagttc
     2881 atggtatctt gcaacaaggc cgctaactgg taaattcaga ggaggagcac acgggtacga
     2941 caacaggaat cctgatatgt acggaatatt ttatgctgca ggacctgctt ttaaaagggg
     3001 tgt
//
LOCUS       DAOSNB010000300         2999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_32874_length_2999_cov_4.085598, whole genome shotgun sequence.
ACCESSION   DAOSNB010000300 DAOSNB010000000
VERSION     DAOSNB010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32874_length_2999_cov_4.085598"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLG42_10200"
     CDS             <1..397
                     /locus_tag="PLG42_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOU31620.1"
                     /translation="EITKYQKSGLKEEQADEYLKILLDCMEKEKPYLNNNLSINDLSS
                     LTGIPRHHITQVLNEKYGKNFFTFINEYRVKEVISRLSDPRYNSYTLLAIAYDSGFNS
                     KATFNSFFKSYTGLTPSEYREKLAGSSGS"
     gene            515..1909
                     /locus_tag="PLG42_10205"
     CDS             515..1909
                     /locus_tag="PLG42_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31621.1"
                     /translation="MRFGKIEVFILASIIKGTLLSVYCLNGQTEVQNQTLSFGGFVRG
                     GAYYDLNRQNDKPLFSSGFSDLALKLEARQEDKMRAFSDIRLRYGSEFNNSVNSITIR
                     EAFTEVYFPFFRFSIGQQIIKWAKTDFTTTNSKLNPQNFTTRSPDREDMDLGNIIASL
                     SWYPSPIFDFQAVYIPFYRPSVLLVDQVPIPEGVEIKSLKSLVTDENYSGYGFKGSIH
                     IRGADLGVTWFDGYDPMPGIKLDEFSLDMSGPVPVASTKLSVTPYKIRSAGIFFEAVT
                     GEICTRGEVALSDPYADYRENEYVPAPEVKWVGGFDYTPGDWRIVFEYSGKYITRFTR
                     PDTEPLIGTEPDYTQLLQLISTPGFDIKEYVRKQTEAFNRLYNYQLEKSYHSAGLRLE
                     KEIAYGKYVTSLSGLYNFTAHELLLIPEIKFKPYDAITITAGAEIYSGRKGSLYKLVN
                     DFMESIYAGIRIDF"
     gene            1921..>2999
                     /locus_tag="PLG42_10210"
     CDS             1921..>2999
                     /locus_tag="PLG42_10210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOU31622.1"
                     /translation="MSRFIIRFRWLIISVCLITGCFFIFLIPKASTEPDIRNYIPSNL
                     QSKIRTDSIESVFGFQDMVMILYRDTSSVITGNNLTTLKRIEEQLSQLSEISSIISPF
                     SIKAIRSEEGYMVAERLVGQIPSNRKEEEELISRINSNSYSRDIVFSPDLKTAAITLT
                     LSNKTHETELLNKIDSVIISSGESNKVMKGGLPVIRKFILKDVRRDAIIVVPVALFIM
                     LLILRLTLGSWRNVYIPFSVVILSTGISMGLIPLLGWKLSIITLLIPIILIAVANNYG
                     IYLVSYYNRVANTIKGSKYEILKEVMGSLRIPVLFSGLTTIAGILGLLAHSIIPARQV
                     GVLAACGISIALAMSFFMVPALLSV"
BASE COUNT          936 a          536 c          595 g          932 t
ORIGIN      
        1 ggaaattaca aagtaccaga agagtggact gaaagaagaa caggcagatg aatatctgaa
       61 gattcttctc gattgcatgg aaaaggaaaa gccttatctc aataataatt taagtatcaa
      121 cgatctatct tctttaacgg gaataccacg tcatcatatc actcaggttc tcaatgaaaa
      181 atatggtaag aattttttta cgtttataaa tgagtacagg gtgaaagagg taatctcaag
      241 gctttctgat ccgagatata acagttacac ccttcttgct attgcatatg attcgggctt
      301 caattcaaaa gccacattta actccttttt taaaagctat actggcctca ctcccagtga
      361 gtacagggaa aagttagctg gcagctccgg ttcctgagca gagtctttcc ctgcaagatt
      421 gaaccagtaa gtttcttttt cccggtatta aaaaatcgga ttggacgacc gtttattctt
      481 caatctcttt ctttgaccga aaaaaaagca aggcatgaga ttcggcaaaa tagaggtttt
      541 catcttagca tctattataa aaggcacatt attatctgtt tactgcctga atggccaaac
      601 tgaagtacaa aaccagacac tttcttttgg aggttttgta agaggtggtg catattatga
      661 tcttaaccgt cagaatgaca agcctttgtt ttcaagcgga ttttctgatc tcgctttaaa
      721 gcttgaagcc cggcaggaag ataaaatgag ggcattttcg gatatcaggt tgcgatacgg
      781 ctctgagttc aacaattctg ttaattctat aacaataaga gaggctttta cagaagtcta
      841 cttccccttt ttcaggttct caataggaca gcagattata aaatgggcaa aaactgattt
      901 tacaaccaca aattcaaagt taaacccgca aaatttcaca acaagatccc ccgacaggga
      961 ggatatggat cttggcaaca taattgcctc attatcatgg tatccttctc ccatatttga
     1021 ttttcaggca gtatatatcc ccttttatcg tccatcggtt cttcttgtag atcaagtgcc
     1081 tattcctgaa ggagttgaaa ttaaatcgtt aaaatcactt gttaccgatg aaaattattc
     1141 gggatatggt tttaagggga gtattcacat cagaggtgca gatctaggcg ttacatggtt
     1201 cgatggatat gatcctatgc cagggattaa gcttgatgag ttttctcttg atatgtcagg
     1261 tcctgttcct gttgcttcga caaaactgag tgtgactcca tataaaataa ggagtgcagg
     1321 aatattcttt gaagcagtga ccggagaaat atgtacgagg ggtgaagtcg cattatccga
     1381 tccttatgct gattacagag aaaatgaata tgttccagct cctgaggtaa aatgggttgg
     1441 agggtttgat tatacaccgg gagactggag aattgttttt gaatattctg gaaaatacat
     1501 tacacgcttc accaggcctg atacagaacc cttaattggc actgagcctg attatacaca
     1561 gcttcttcaa ttgatttcca cgcctggttt tgatataaag gaatatgtaa gaaaacaaac
     1621 agaagctttc aacagacttt acaactacca gctggaaaaa tcatatcatt ctgcaggttt
     1681 gaggctcgag aaagaaattg catatggtaa atatgtcact tctttatctg gtttatacaa
     1741 ttttacggct catgaactct tattgattcc cgaaattaag ttcaaaccat acgacgctat
     1801 tacaattaca gccggagcag aaatatattc aggcaggaaa ggttctttat acaagcttgt
     1861 aaatgacttt atggaaagca tttatgcagg aattagaatt gatttttaat catattatat
     1921 atgagtagat tcatcataag gttcagatgg ctgataattt cagtttgttt aattaccgga
     1981 tgttttttca tctttctcat tcctaaagct tcaactgaac ccgatatcag aaattatatt
     2041 ccttcgaatc tgcagtcgaa aataagaact gactcgattg agagcgtatt cggatttcag
     2101 gatatggtga tgattcttta cagagacacc tcttcagtga ttacgggaaa taatcttacg
     2161 acacttaaga gaattgaaga acaattatca caattaagcg aaatttcgtc catcatctcc
     2221 cctttcagca ttaaggcaat aagaagcgag gaaggttaca tggttgctga aaggcttgtt
     2281 ggacaaatac cttccaacag gaaagaagag gaggagttaa tttccagaat taattcaaat
     2341 agttactcac gtgatatagt tttttctcca gatctaaaga cagctgctat aacacttact
     2401 ctttccaata aaacacatga aacagaatta ttgaacaaga ttgattccgt cattatttca
     2461 tcaggtgaga gtaataaggt tatgaaaggt ggtttacctg taataaggaa atttatactt
     2521 aaagatgtaa gaagggatgc cataattgtt gtccctgttg cactatttat aatgctttta
     2581 attctgcgcc taacactcgg gtcatggagg aatgtttata ttccattttc tgtggtgatt
     2641 ctgtcaacag ggataagcat ggggcttata ccactgctgg ggtggaaatt gtcaattatt
     2701 actcttctca ttccaataat acttatagcc gtagctaaca attacggaat atatcttgta
     2761 agttattata accgggttgc aaatacaatt aagggcagca aatatgaaat ccttaaagaa
     2821 gtaatgggtt ctttaaggat acctgtgtta ttcagtggac tgaccacaat agcaggaata
     2881 cttggtttgc tcgctcattc aataattccc gcaaggcagg ttggagtgct tgcggcatgt
     2941 ggcatttcca tagcattggc aatgagtttc tttatggtac ctgctctgct atctgtcag
//
LOCUS       DAOSNB010000301         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_33338_length_2967_cov_4.047390, whole genome shotgun sequence.
ACCESSION   DAOSNB010000301 DAOSNB010000000
VERSION     DAOSNB010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33338_length_2967_cov_4.047390"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLG42_10215"
     CDS             <1..587
                     /locus_tag="PLG42_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31623.1"
                     /translation="NNPKELKVYVRETDTARPVYESELISESTSLLDVMELFNSADHF
                     FVKSGHRITHIVTVSDLDKLPVRLFMFGILCLFELKIREIIRQRKIKWEDLISNEKLE
                     EIKRRYENKREKDEETTLLDCTDFGSIVYVILGSKKDFAGYFPVNFFGNGKDEKLLKD
                     AIRLRNAIAHGGIIEFDHQRVNEIIKLLKGVIEK"
     gene            complement(645..992)
                     /locus_tag="PLG42_10220"
     CDS             complement(645..992)
                     /locus_tag="PLG42_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31624.1"
                     /translation="MEKNIKYRLWESNEFLDFVRGDSCESRSFFWRDMEELRKKLDEQ
                     KELRRILMSGKNVIVEKGYYIGDRFFLVNWAYSFVQAKGVFMFGIVRVLETECGSENH
                     PLLVNAFIPVNFQ"
     gene            complement(994..1992)
                     /locus_tag="PLG42_10225"
     CDS             complement(994..1992)
                     /locus_tag="PLG42_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4407 domain-containing protein"
                     /protein_id="HOU31625.1"
                     /translation="MKDFMLRFECFLTGYNYEIIKNSCEASRKAVKRFFSSVLIISML
                     WGAIGYAFARRYLKFDVSGAIVFSAIAIFCVVNIERIIILSGKKNVIRGFRIFLGILM
                     ALIGSLITDQFIFREDIEKKKISMLQEEVNAILPKKTLELDNQIRHIDSAILMKEAER
                     SAIIEELSVRPLIKSTSAEKRHFALRQNGSNGEARDTLVARTDYVLTDIPNPKANLLP
                     GLADQITLLRNQKAEKENSKINIRQEIEDELSSKTGFLDELNVLFALLFSSPVAMAVW
                     IFFLLFFITIELFVVMNKFGEEDTDYDAVIAYQKDVKIKSLQNLKEESYQKTRILN"
     gene            complement(2005..2559)
                     /locus_tag="PLG42_10230"
     CDS             complement(2005..2559)
                     /locus_tag="PLG42_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31626.1"
                     /translation="MKLFELFSKKISGNGKELPAPENGSPVVPESLFIDNSEPVDTST
                     HSENSVPAGMEEVYSFLLADYEEKGYNDALTNPDTGYRDDNIRLLKEEFGILIKKTIL
                     NFEVQLNELEFHIETRSRAGLIDLVEELKARRNIIFRKLEDIIRLKQEEGNGAVERMV
                     LSYKRGFMRGLAAITKSELIRKNI"
     gene            complement(2585..2905)
                     /locus_tag="PLG42_10235"
     CDS             complement(2585..2905)
                     /locus_tag="PLG42_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOU31627.1"
                     /translation="MDEAKTYHGYQKEPDKPSVHGSYGSALFDPRWKAARERILARDK
                     QRCLNCGSTENLQIHHRQYHFSKSLNDFVRPWEYNQKYLITLCESCHSKGHRLYRVPV
                     KTVK"
BASE COUNT          855 a          649 c          518 g          945 t
ORIGIN      
        1 ataataatcc aaaagagttg aaagtttatg tgcgagaaac agatactgcg agacctgttt
       61 atgagtcgga actgatatca gaaagcactt cactgctcga tgtgatggag ctcttcaata
      121 gcgccgacca tttctttgtt aaatccggtc acagaattac acacatagtt acagtatccg
      181 acctcgataa gttgcctgta cgactcttta tgttcggtat tttatgcctt ttcgaattaa
      241 aaataaggga gataatacgg caaaggaaaa ttaaatggga agatctcatc agcaacgaaa
      301 aacttgaaga gataaagaga aggtatgaaa ataagagaga aaaagacgag gaaaccactt
      361 tgctcgattg tacagatttt ggcagtattg tttatgtaat ccttggaagt aaaaaagatt
      421 tcgcaggcta tttccctgta aatttttttg gaaacggaaa agacgaaaaa cttctgaaag
      481 acgcaatacg cctgcgaaac gctattgcgc acggagggat tatagaattt gaccaccaac
      541 gtgtaaatga aatcataaaa ctgcttaaag gggtgattga aaaataatat atattctgcc
      601 acggattttt acctgacatc ctgtgtaaaa atctcccggc cactctactg aaaattgacc
      661 ggtataaaag cattgacaag caaagggtga ttttccgaac cacattcggt ctcgagaact
      721 ctcacaattc caaacatgaa aacccccttt gcctgtacaa acgaatatgc ccagtttaca
      781 aggaaaaacc tgtcgccaat gtaatatcct ttctcaacta ttacattttt ccctgacatg
      841 agtatccttc tcagttcctt ctgctcatcg agcttttttc tcagctcttc catgtctctc
      901 cagaagaaac tccgggattc acaggaatct cccctgacaa agtcaagaaa ttcattgctc
      961 tcccatagcc gatattttat gtttttttcc atatcagtta agtattctgg ttttttgata
     1021 actttcttcc tttagattct gaagactttt tatctttaca tctttctgat acgcaataac
     1081 tgcatcataa tcagtatcct cttcaccaaa cttgttcata accacaaaca gttcaattgt
     1141 gatgaagaat aacagaaaaa atatccatac tgccatagcc acaggcgatg agaaaagaag
     1201 tgcaaacaac acattcaact cgtcgagaaa gccggtcttc gatgaaagtt catcctcaat
     1261 ctcctgtctt atgttgattt tggaattctc cttctcagcc ttctgattcc tcagaagcgt
     1321 aatctgatct gccaaacctg gcaaaagatt cgcctttgga ttgggaatgt cggtcagaac
     1381 atagtcggtg cgagccacaa gagtatccct ggcttcgccg tttgagccgt tctgcctcaa
     1441 tgcaaaatgc cttttctcag ccgatgtgct ctttattaaa ggacgtactg acaactcttc
     1501 gattatcgca ctcctctcgg cttccttcat tagaatggcc gaatcaatat gccttatctg
     1561 gttatcaagc tccagcgtct ttttcggaag gatggcatta acttcttcct gaagcatgct
     1621 gatcttcttt ttctcaatgt cctccctgaa gataaactgg tcagtaatta atgaacctat
     1681 taatgccatc agtattccga ggaaaattct gaaaccacgt attacgtttt tctttcctga
     1741 aagaattata atcctttcaa tatttaccac acagaatatg gcaatggccg aaaaaactat
     1801 tgctcctgaa acatcaaatt tcagataacg cctggcaaaa gcatatccta ttgcacccca
     1861 gagcatgctt attattaaaa cggagctgaa aaatcttttc accgcttttc tgctggcttc
     1921 acatgagttc tttattatct catagttgta gcctgttaaa aaacattcga atcttaacat
     1981 aaagtctttc ataattctga agttttaaat gttttttctg attaattctg attttgttat
     2041 agctgcaaga cctctcataa atcctctttt ataggataat accatccttt cgacagcacc
     2101 attgccctct tcctgcttta gccttattat gtcttcaagc ttcctgaaaa ttatgttacg
     2161 ccttgcttta agttcctcaa caagatcaat tagtcctgca cggctgcgag tttcaatatg
     2221 gaactcaagt tcattaagtt gaacctcgaa atttagaatg gttttcttaa taagaattcc
     2281 aaactcctct ttcaacagcc tgatgttatc atccctgtat ccggtatccg gatttgtcag
     2341 ggcgtcgttg tatccctttt cctcataatc ggcaagaagg aatgagtaaa cctcttccat
     2401 acctgccggt acactatttt ctgagtgtgt gctggtgtca acaggttcac tgttatcaat
     2461 gaacaggctc tcgggcacaa caggtgaccc gttttctggt gcgggaagct ccttcccgtt
     2521 accggaaatc tttttactga ataattcgaa tagtttcata atttctgttt tttattgtta
     2581 ttaattattt tactgttttt acaggcaccc tgtaaagcct gtgaccttta ctgtgacagc
     2641 tttcacacag ggtgataaga tacttctgat tatactccca gggtctgacg aaatcgttga
     2701 gcgacttcga aaagtgatac tgcctgtgat gaatctgaag gttttcggtc gaaccacaat
     2761 tcagacacct ttgtttgtca cgggcaagta tcctctccct tgctgctttc catctcgggt
     2821 caaataacgc cgaaccatag cttccgtgca ctgacggttt atcaggttct ttctggtatc
     2881 catgatatgt ttttgcttca tccatgatat ttattttttg ctctaccaat atctattatt
     2941 atgcctgttt tttgacaaaa aaataaa
//
LOCUS       DAOSNB010000302         2930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_33850_length_2930_cov_4.151304, whole genome shotgun sequence.
ACCESSION   DAOSNB010000302 DAOSNB010000000
VERSION     DAOSNB010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33850_length_2930_cov_4.151304"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1435
                     /locus_tag="PLG42_10240"
     CDS             233..1435
                     /locus_tag="PLG42_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOU31628.1"
                     /translation="MRINDIIKVIVLFSVIAVACNKPAPMEKVIEKSLAVAEKQYIQM
                     AEVMKQKPDLLPRTLDTAGNLVTAKSNWWTAGFFPGTLWYLYEYTGDEKFKDYAREMT
                     ARVEKEKFNRGTHDLGFMLYCSFGNGFRLTGDESYKDVMLTGAQSLITRFRPKVGCIQ
                     SWNSRRGWQCPVIIDNMMNLEFLMWAFKTTGDSVYYKICVSHSDTTIKNHFRPDYSTW
                     HVVSYDTITGKVEAKQTAQGYSDESAWARGQSWGLYGYTVMYRETGLERYLEQAKHIA
                     DFLINHPNLPEDKIPYWDYNAPNIPNELRDASAGAIMASALIELSGYVDKETGKKYLD
                     VAEKQLRTLASPEYLAKPGENGNFILKHSVGSLPGKSEVDVPLTYADYYFVEALLRYK
                     NLLKNQQI"
     gene            1481..2803
                     /locus_tag="PLG42_10245"
     CDS             1481..2803
                     /locus_tag="PLG42_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOU31629.1"
                     /translation="MKQGKKLSRREFLGASALGMAGIMILPAASCGRSKTEKAPSDLV
                     RLGFIGLGRQAMFLLNGFLQVPGVEVLAGCDVYGVKRTRFERRVNEFYSKAGKKVEVK
                     TYEKFQDLLARKDIDAVVIATPDHYHALIAIAACKAGKDVYLEKPLTFTIYEGQQLVK
                     AVRENNRILQVGSQQRSDDTFRHAVNLVQSGAIGKITLVNAYVGAPPTPYNLPPEPVP
                     ADLNWDLWLGPLPANIHYNSQLNPPISLDPPKDEEIWGAWRWYKETGGGFTTDWGAHM
                     FDIAQWGLGMDNSGPVEASPIGDGTEFMSFKYANGVVLTSETWDEKKTKGVKFHGENG
                     WIEVARGYFNASDPKLFPEKKEEQTNMPYETRIPHQVNFIESVRTRKDPVATVEIGHR
                     TCTVCNIGNIACDLKRTVKWNPETETFVDDEEATKRMHYTYREPWKLV"
BASE COUNT          924 a          583 c          671 g          752 t
ORIGIN      
        1 caacaggaat ttctgatgat agaaatataa gcaatattgc cgtaaaaaag agatatacaa
       61 catggaagta tttatatttc ataattttat aaatttacat tgtattaatt tcagtcaaaa
      121 tctgaaaatt cgataaacaa aaataacata atgcccatta aatttgattg tagtgtagtt
      181 ttaaatgata atttttatat ttacgaatta aaaattgatt aaaatttcag ttatgagaat
      241 taacgatatc attaaagtta ttgttttatt ttcagttatt gccgttgcct gcaacaagcc
      301 tgcaccgatg gaaaaagtta ttgaaaagtc actggctgtg gctgagaagc aatatattca
      361 gatggcagaa gtgatgaaac aaaagcctga tcttcttccg cgtactcttg atactgccgg
      421 taatctggtt acagcaaaat cgaactggtg gacagcaggt tttttccctg gaacactgtg
      481 gtatctgtat gagtatacag gtgatgaaaa attcaaggat tatgccaggg agatgacagc
      541 cagagtagaa aaggaaaaat tcaacagagg aacgcacgat ctggggttca tgctctattg
      601 cagctttggt aacggattca ggttaacagg tgatgaaagt tacaaagatg ttatgcttac
      661 cggggcacaa tcgctgatta caagattcag gcctaaggtg ggttgtatac agtcgtggaa
      721 cagtcggcgt ggctggcagt gccctgttat aatcgacaac atgatgaacc ttgagtttct
      781 gatgtgggca ttcaaaacaa ctggcgattc ggtatattat aagatatgtg tcagtcactc
      841 agatactacc attaaaaatc attttcgccc cgattacagc acctggcatg tggtctcata
      901 tgataccata actggtaaag tggaagcaaa acagactgca cagggctatt ccgatgagtc
      961 ggcatgggca cgcggacaat catggggtct ttacggctat accgttatgt accgtgaaac
     1021 gggactcgaa agatatcttg aacaggcaaa acatattgca gattttctga ttaatcatcc
     1081 taacctgcct gaagacaaga tcccttactg ggattataat gcaccgaaca taccgaacga
     1141 attacgtgat gcttcagctg gagctattat ggcatcggca ctgattgagt taagcgggta
     1201 tgttgataag gagacaggga aaaaatatct ggatgttgct gaaaaacagc tcagaacact
     1261 ggcttcgccc gaatatctgg ctaaaccggg tgagaatggc aattttatcc ttaaacatag
     1321 tgtgggttca ctccctggaa aaagcgaagt ggacgtacca cttacttatg cagattatta
     1381 ttttgtagaa gctctgctca gatataaaaa tctgctgaaa aatcaacaaa tttgaataaa
     1441 tttttaatta gctgaaagat aaccatttaa ctaaactgac atgaaacagg gaaaaaaact
     1501 ttcaagacgt gaatttctgg gagcatcagc tctcggtatg gcaggcataa tgatactgcc
     1561 tgctgcaagc tgcggacggt ccaaaacgga aaaggccccg tccgatctgg taagactggg
     1621 ctttattggt ctgggccgtc aggcaatgtt ccttctgaat ggcttcctgc aggtacctgg
     1681 tgttgaggtt cttgcaggct gcgacgttta tggtgtgaaa cgcaccaggt ttgaaaggag
     1741 agttaatgaa ttctactcca aagcaggcaa gaaggttgaa gtgaagacat atgaaaaatt
     1801 ccaggatctt cttgcccgca aggacattga tgctgtggtg attgcaacac ctgaccatta
     1861 tcatgcacta attgcaattg ctgcatgcaa ggccggcaag gatgtctacc tcgagaaacc
     1921 tctgaccttt acgatttatg aaggacagca gcttgtcaag gctgttcgtg agaacaacag
     1981 aatactccag gtaggcagcc agcagcgctc tgacgatact ttcagacatg ctgtaaatct
     2041 tgttcagtct ggagctatag gcaagataac acttgtcaat gcttacgttg gtgcgccacc
     2101 aacaccctac aatctgccac ccgaacctgt accagccgac cttaactggg atctctggct
     2161 tggtccattg ccagcaaata ttcattacaa cagccagctc aacccgccca tatcactcga
     2221 tccgccaaaa gacgaagaga tatggggtgc ctggagatgg tacaaagaaa caggaggcgg
     2281 ctttacaact gactggggtg cccatatgtt cgacatagca cagtggggcc ttggaatgga
     2341 taacagcgga ccggttgaag cttcaccgat tggagatggc accgagttta tgtcgttcaa
     2401 atatgcaaac ggagtggtgc ttacttccga aacatgggac gagaagaaga ccaaaggtgt
     2461 caaattccat ggtgagaatg gttggataga ggtagcccgc gggtatttca acgcatccga
     2521 cccgaaactc ttccccgaaa agaaggaaga acagacaaac atgccatacg aaaccagaat
     2581 accccaccag gtgaacttta tcgagtcggt gcgcaccaga aaagatcccg ttgcaacagt
     2641 ggaaataggc caccgcactt gtacagtatg caatatcggt aatattgcat gcgaccttaa
     2701 acgtactgta aagtggaatc ctgaaaccga aaccttcgtc gacgacgaag aggcaacaaa
     2761 gaggatgcat tatacctaca gggaaccctg gaaactggtt taatgcaacc cttcccatac
     2821 caataaaaaa ccccttccgg agaaaccgtg aaggggtttt tattatctcg ataattaaat
     2881 gtgtctggaa cggttgaaaa attataaaag tatttcttaa aaatttatta
//
LOCUS       DAOSNB010000303         2871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_34793_length_2871_cov_5.013494, whole genome shotgun sequence.
ACCESSION   DAOSNB010000303 DAOSNB010000000
VERSION     DAOSNB010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34793_length_2871_cov_5.013494"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLG42_10250"
     CDS             complement(<1..598)
                     /locus_tag="PLG42_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOU31630.1"
                     /translation="MDSKKIIRTTEDFVVNNITKHDSAHDWWHIDRVRRMALFICKEE
                     KSGNPLIVEVSALLHEIGDRKFNDDSGEDVISKMRKMLLETGLNEKMINEIIFISQNI
                     SFSKGYKGTGITDEFKIIQDADRLDAIGAIGIARAFSYGGFTGVPLFDPEGHRESTIK
                     HFYDKLLKLKDLMNTETAKKLAEERHIYMAGFLDRFYQE"
     gene            679..1920
                     /locus_tag="PLG42_10255"
     CDS             679..1920
                     /locus_tag="PLG42_10255"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOU31631.1"
                     /translation="MKAEIITIGDEILIGQTVDTNSAWIGSELSKLGFEITQITSIPD
                     RRESILKILSEVTGRADVVIITGGLGPTSDDITKQTLCEFFNTTLVTNQEVLGMIELM
                     FQRRNLPMTENNRRQADVPASCRVLFNAAGTAPGMWFEKGGTIFISLPGVPLEMKYIM
                     NEHVLHELKKRFSSRIIIHKNIMTYGTFEARLAELLVDFEAQLPSNIKLAYLPSLGII
                     KLRLTGSGNNLVELSKQINEQVQKLYATIPDYIYGEDEETLEMVLGKILKEKQLTICT
                     AESCTGGMVAHMITSVPGSSTYYKGSVIAYDNSIKKNILKVKEKTLKENGAVSREVAE
                     EMAAGALKLFDCDFAVATTGIAGPDGGTPEKPVGSVWISVASKNGVTAEKYQFLHDRE
                     TNIKHFSIAALNMLRLRVLVK"
     gene            2033..2275
                     /gene="rpmB"
                     /locus_tag="PLG42_10260"
     CDS             2033..2275
                     /gene="rpmB"
                     /locus_tag="PLG42_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOU31632.1"
                     /translation="MARICQITGKKAQRGHNVSHSKRRTKRLFLPNLFEKKYFLPEEN
                     RWIKLRVSAAGIRLIDKKGLSAALKEAKEKGYIANY"
     gene            2290..2478
                     /gene="rpmG"
                     /locus_tag="PLG42_10265"
     CDS             2290..2478
                     /gene="rpmG"
                     /locus_tag="PLG42_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOU31633.1"
                     /translation="MAKKGKGNRVQVILECTEHKESGLPGTSRYITTKNKKNTPDRLE
                     RRKYNPILKRYTIHREIK"
     gene            2487..2642
                     /locus_tag="PLG42_10270"
     CDS             2487..2642
                     /locus_tag="PLG42_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOU31634.1"
                     /translation="MAKKVVATLKTGVGKHFVKCIKMVKSEKSGAYIFKEEVVHYDHV
                     KDFFNKK"
     gene            2729..>2871
                     /locus_tag="PLG42_10275"
     CDS             2729..>2871
                     /locus_tag="PLG42_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004352230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOU31635.1"
                     /translation="MSLFGFIGKKEKENLNKGLEKTRESVFARISRAIAGKSHVDDEV
                     LDNL"
BASE COUNT         1025 a          507 c          554 g          785 t
ORIGIN      
        1 actcctggta aaacctgtca agaaacccgg ccatatatat atgtctttcc tcggcaagct
       61 tttttgcagt ctctgtattc atcaaatcct ttagcttgag cagtttgtca taaaagtgct
      121 ttattgtcga ctccctatgt ccttctgggt cgaaaagggg tacacctgtg aatcctccat
      181 acgaaaaagc cctggcaata ccgattgccc ctatggcatc cagtctgtca gcatcctgaa
      241 taatcttaaa ctcatctgta atccctgttc ccttataccc tttgctgaac gaaatgttct
      301 gactgataaa aattatctca ttaatcatct tttcattaag tccggtctcc aaaagcattt
      361 ttctcatttt actgattaca tcttcgcccg aatcatcatt aaattttcta tcgcctattt
      421 catgaagcaa tgcagaaact tcaacaatca aaggattacc cgatttttct tccttgcaaa
      481 taaagagtgc catccgcctg accctgtcaa tatgccacca atcatgagca ctgtcatgct
      541 ttgtaatgtt gttaactaca aaatcttccg tggtccggat aatttttttc gaatccattt
      601 gtcaagtgat ttattgatgt ttgaacttac aaaatatatt tttgcaataa aaacattcca
      661 gaaataaacc acagagaaat gaaagcagaa attatcacaa taggtgatga aatcctgata
      721 ggtcagactg ttgacacaaa ttctgcctgg atcggatctg aattaagtaa actgggtttc
      781 gaaataactc agatcacttc aatacccgat cgtcgtgaaa gtattcttaa aatcctttca
      841 gaagtgaccg gcagagcaga tgtggttatt attaccggtg gacttggacc taccagcgat
      901 gatataacaa aacaaacatt atgtgaattt tttaatacta cacttgtaac aaaccaggaa
      961 gttctgggaa tgattgaact tatgtttcag cgaaggaatt tgccaatgac tgaaaataac
     1021 agaaggcagg cagatgttcc agcatcatgc agggtactct tcaatgccgc tggaacagca
     1081 ccgggtatgt ggttcgaaaa ggggggaacc atttttattt cactgccagg tgtgccactg
     1141 gaaatgaaat atattatgaa tgaacatgta ttacatgaac tgaaaaagag gttcagctca
     1201 cgtattataa ttcataaaaa cattatgacc tatggcacat ttgaagcccg cctggcagaa
     1261 ctgcttgtcg attttgaggc acaacttcct tccaatataa aactggcata tttaccttct
     1321 ctgggaataa ttaaactgag gctcacaggc tcaggaaata acctggttga attgtcgaaa
     1381 cagataaatg aacaggtaca aaaactttat gccacaatac ctgattatat ctatggtgaa
     1441 gatgaagaaa cacttgaaat ggtattggga aagatattaa aagagaaaca attaacaata
     1501 tgcactgctg aaagctgtac aggtggaatg gtagcacaca tgataaccag cgtacccgga
     1561 agctcgacat attataaagg ttcagtaata gcatatgata acagtatcaa aaaaaatatt
     1621 ctgaaagtca aggaaaaaac cctgaaagaa aatggtgctg ttagcaggga agtagctgaa
     1681 gaaatggcag ctggagcact taagttgttt gattgcgatt tcgctgtagc tacaacaggt
     1741 atagcaggac ctgacggagg tacgccagaa aaacctgttg gttctgtctg gatatcagta
     1801 gcttcaaaga atggcgttac tgctgaaaaa tatcagttcc tgcacgacag ggaaacaaac
     1861 ataaaacatt tctctatcgc agcacttaat atgctccgtt tgcgagttct agtcaaataa
     1921 tttgtaaaca ctgtttaaaa aagtttaata aaaatttgtt taatccaata aaaatgacgt
     1981 aatttgcgcg tcaattttat gaataaattt ttaacaggaa atagacatta aaatggcacg
     2041 tatctgtcag ataacaggga aaaaagctca gaggggtcat aatgtatccc actcaaaaag
     2101 aaggacaaaa agattgtttc tcccgaatct ttttgagaaa aaatattttt tacctgaaga
     2161 aaacaggtgg ataaaactga gagtcagtgc agcaggcatc cggctgattg acaaaaaagg
     2221 actgagcgct gcattgaaag aagctaagga aaaaggctat attgctaact attaaaaaag
     2281 aaatacaaca tggcaaaaaa aggaaaagga aacagggtac aggtaattct cgaatgcacc
     2341 gaacataagg agagtggttt gccaggtact tcaaggtata taacaacaaa aaacaaaaaa
     2401 aatactcctg atcgtttgga gaggagaaaa tataacccga ttctaaaaag gtatactatt
     2461 cacagggaaa taaaataatc tgagatatgg caaagaaagt agttgcaaca ttaaaaacag
     2521 gtgtaggtaa acactttgtt aagtgcatca agatggtaaa atctgaaaaa tcaggggctt
     2581 atatattcaa ggaagaggtt gtccattatg accacgtaaa agattttttt aataagaaat
     2641 aaattaaaac actgacccga aaaaaagctt tcttcaacat gaagagggct ttttttgtca
     2701 ttatcaaagc ctgcttagaa aatacaaaat gagtttattc ggattcatag ggaaaaaaga
     2761 aaaggagaac cttaataagg gtctggaaaa aactagagag agtgtatttg ccagaatttc
     2821 aagagcaata gcaggaaaat cacatgttga tgacgaagtt ttagataatc t
//
LOCUS       DAOSNB010000304         2829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_35463_length_2829_cov_5.143115, whole genome shotgun sequence.
ACCESSION   DAOSNB010000304 DAOSNB010000000
VERSION     DAOSNB010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35463_length_2829_cov_5.143115"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..992)
                     /locus_tag="PLG42_10280"
     CDS             complement(183..992)
                     /locus_tag="PLG42_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOU31636.1"
                     /translation="MKNYFHFAGKKIFYTDQGRGPVIVLIHGYLETSEVWEDFAGSLA
                     DSFRILAIDLPGHGNSDIFNHTHTMEFMADVTEALLNVTGTDRAFVTGHSMGGYVTLA
                     FLEKYPHKLTGYCLFHSHPFADTPEVLKRRENDIKMVEGGKKFMLYPDAVKRMYADMN
                     LEKFHDAFEKSKRIASGIRDEGITAVLRGMMARPSRVHLMEAGKVPCLWILGAKDNYI
                     QCESMKTRVKLPPNAELIILENSGHMGFIEEKEKSLEVMKNFLRKIDFKSF"
     gene            1161..1805
                     /locus_tag="PLG42_10285"
     CDS             1161..1805
                     /locus_tag="PLG42_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOU31637.1"
                     /translation="MYKILFSSVSKKFVMAVAGLFLILFLPVHLTINLMLLKSDPLPF
                     NRAAHFMASFPVIKVFEVILFITLIVHIFYGIWLQIQNWMARPIRYASGQKSETSSFS
                     RFMIWTGLTVLIFLIIHFFNFFFIKLGIVEGNPEDFYSVAHELFKIPAYNYIYLACFL
                     LLGFHLHHAFSSAFQTLGLNHRIWTPVIKVLAWVYSIAIPAGFATIAIVLWQLK"
     gene            1824..>2829
                     /locus_tag="PLG42_10290"
     CDS             1824..>2829
                     /locus_tag="PLG42_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOU31638.1"
                     /translation="MTILNSKIPEGPLSEKWTRYKENVKLVSPANRKKLDIIIVGTGI
                     SGAAAASALGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDGDSVYRLFYDMI
                     KGGDYRSREANVYRAAEVSNQVIDHFTALGVPFARDYGGTLDTRSFGGVQVSRTFYAK
                     GQTGQQCLLGCYSSLNRQIKKGAVTMFPRREMLDLVVVEGRARGIITRNLITGEIERH
                     SAHAVVLATGGYGTIYYLSTLAVNSNPSAAWKAYKRGAFFANPSFIQIHPTCVPQMND
                     YQSKLTLMSESLRNDGRMWVPKTKGDTRNPNDIPEDERDYFLERRYPAFGNLVPRDVG
                     S"
BASE COUNT          825 a          601 c          523 g          880 t
ORIGIN      
        1 taccgattct gcgcatttta accggtcgtg gcgacattat tacaattaaa aatccgtcac
       61 aaaaataatg aacttatgtt cataatccaa atcaataaca atttttttat ctttatttta
      121 accctgataa attaacggag agtacaatta agacataaaa atctgaaggg tagctgataa
      181 aatcaaaaag acttaaaatc tatttttctt aaaaaatttt tcattacttc caacgatttt
      241 tccttttcct caataaagcc catatgacca gaattctcaa gtattatcag ttcagcattc
      301 ggtggcagtt taacccttgt cttcatcgat tcgcactgga tataattatc ttttgctccg
      361 agtatccaga ggcatggaac ctttcctgct tccattaaat gaaccctcga aggtcttgcc
      421 atcatccctc ttaatactgc ggttattcct tcatccctta tccccgatgc aatcctcttt
      481 gacttttcaa aagcatcatg aaatttttca aggttcatat cagcgtacat ccgctttaca
      541 gcatcgggat aaagcatgaa tttcttacct ccctcaacca tctttatgtc attttctctc
      601 ctttttaaaa cttcgggagt atcagcaaaa ggatgtgaat gaaacaaaca gtagcctgta
      661 agtttatgag gatatttctc cagaaaagca agcgtaacat atccacccat tgaatgacct
      721 gtaacgaagg ctctgtcagt tccggttaca ttgagaaggg cttcagtaac atcggccata
      781 aattccattg tgtgagtatg attaaatatg tccgaattgc catgccccgg aagatcaata
      841 gcaagaatcc tgaagctgtc tgccagggat ccggcaaaat cttcccatac ttctgaagtt
      901 tcgagataac cgtgtatgag tacaattaca ggccctctac cctggtcggt ataaaaaatt
      961 ttcttaccag cgaaatgaaa ataatttttc aatttaacta tgttttctcc aaaaatagcc
     1021 aaatggtaat aaatgagcaa tgcagcaaat aatttttttg ccaacaatta gcatgtttat
     1081 ttcataaatt ttaatttaat tttatatcca tcatctggag attgtatttg ttaatttact
     1141 cataaactct tatttagtca atgtataaaa ttcttttttc atccgtttct aagaaatttg
     1201 ttatggctgt tgcaggtctg tttctgatat tattcctgcc tgtacatctg actatcaacc
     1261 tgatgcttct taaaagtgat cctttacctt tcaacagggc tgcccatttt atggcttcct
     1321 tcccggttat taaagtattt gaggtaatac tttttataac acttatagtt catatctttt
     1381 acgggatatg gctgcagatt cagaactgga tggcacgacc cataaggtat gccagtggcc
     1441 aaaagtcaga aacctcctcc ttttcaaggt ttatgatttg gacaggccta actgtactga
     1501 tttttcttat aattcatttt ttcaatttct tttttattaa actgggaatt gtggaaggga
     1561 accctgaaga tttctattct gttgctcatg aactctttaa aattcctgct tataattaca
     1621 tctacctggc ttgttttctt cttcttggat ttcacctgca ccatgcattt tcttcggctt
     1681 tccagacgct ggggcttaac catcgtatat ggactcccgt aatcaaagtg cttgcctggg
     1741 tctattccat tgcaattcct gctggttttg caacaatagc tatagtatta tggcagttaa
     1801 agtaacctga taatttaaaa actatgacga ttttaaactc aaaaataccg gaaggacctc
     1861 tttctgagaa atggacaaga tataaggaga atgttaagtt agttagtcct gccaacagga
     1921 aaaagcttga cataataatt gtcggtacag gaatttcagg tgcagctgct gcatcagctc
     1981 tgggcgagct cggttacaat gtaaaggttt tctgttatca ggattcgccg cgtagagcgc
     2041 attcagtggc agcacaggga ggaattaacg ctgcaaaaaa ttacaaaaac gatggcgatt
     2101 ctgtataccg gctcttttat gatatgataa agggtggcga ttaccgttcg cgtgaagcca
     2161 atgtataccg tgcagcggaa gtcagcaatc aggtaataga ccattttacc gcccttggag
     2221 taccctttgc ccgcgactac ggaggcacac tcgatacccg ttcttttgga ggcgtacagg
     2281 tctcacgaac attctatgca aaaggacaaa caggtcaaca atgccttctg ggatgttact
     2341 cctcgctcaa cagacagatt aaaaaaggtg cggttaccat gtttccacga agggaaatgc
     2401 tcgatctggt tgtagtcgaa ggcagggcaa gaggaattat aacccgcaat cttattaccg
     2461 gcgaaattga aagacactca gcccatgccg ttgttcttgc aacaggcggt tacggcacaa
     2521 tatattatct ttccaccctc gcagtcaatt ccaatccctc tgctgcatgg aaagcctaca
     2581 aacggggtgc cttctttgcc aatccgagct tcatccagat tcatccaacc tgtgtgccac
     2641 agatgaacga ttatcagtca aagctgactc ttatgtcgga gtcgctccgc aatgatggcc
     2701 gtatgtgggt accaaaaaca aaaggagaca caagaaatcc caacgatata cctgaagatg
     2761 aaagagatta ttttctcgaa agaagatatc ctgcatttgg taacctggtg cctcgcgatg
     2821 tgggttcga
//
LOCUS       DAOSNB010000305         2827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_35490_length_2827_cov_3.716811, whole genome shotgun sequence.
ACCESSION   DAOSNB010000305 DAOSNB010000000
VERSION     DAOSNB010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35490_length_2827_cov_3.716811"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLG42_10295"
     CDS             <1..427
                     /locus_tag="PLG42_10295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU31639.1"
                     /translation="RGDGIKFRKEHPVLGTARWHYGQDFLAPYGTEVYATGAGTVVAA
                     GWSPNGFGYHVIIDHGYGFRTIYGHLSKIDVPVGLNVKRGDRIGLSGNSGTSSGPHLH
                     YQIDYNGTHQNPLWFFSDDLTPEEYRDMIMTLNSKSKFR"
     gene            531..1502
                     /locus_tag="PLG42_10300"
     CDS             531..1502
                     /locus_tag="PLG42_10300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOU31640.1"
                     /translation="MGKAKYKFNPESLSFDRITLGFRDILLRFMAYFLGSLFIAAIYG
                     MIFTIFFDSPKEKALKREIEQLTLQYELMNREMDNMEKILDHLKETDDNLYRTIFEAE
                     PVPSTLREGGIGGINRYKELENYSNAKLIIETAKKLDRIKKKIVVQSKSFDELISLAR
                     EKEEMLASIPAIMPVSNKDLTRTASGWGLRIHPIYKIVRFHYGIDFTAPVGTEVYATG
                     DGVVESIISSRRGYGNHIVVNHGFGYKTLYAHLDRFNVRQGQKVKRGDVIGFVGNTGL
                     SIAPHLHYEVELNGIKVDPSNYFFHDLTPEEYERIVEIASRSGQSFD"
     gene            1620..2603
                     /locus_tag="PLG42_10305"
     CDS             1620..2603
                     /locus_tag="PLG42_10305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOU31641.1"
                     /translation="MTAKRFVIAGVGDIMPGTSYPSPKYLPPDNNPYPLIENVADILR
                     EPDITFGNLEGSFLNEGEPLKKCRDTTNCYIFRIPEKYAGILSYAGFDLISIANNHFM
                     DFGSPAVIRTMQLLDSLGIKYAGTEEVPYAIITRDSITVGFCAFSVNKGTLNLNDYET
                     AEKIVRKLDNECNIVIVSFHGGSEGAENQRVTRNREYLHGEDRGNVYEFAHKMIDAGA
                     DVVFGHGPHVTRAIELYKDRLICYSLGNFCTYRRINVAGPNGIAPVVRVETDITGRFI
                     SGDIIPVYHDAERIVRYDPLKRAVFKIRELTLLDFPETGIEITDDGKIIKK"
     gene            2609..>2827
                     /locus_tag="PLG42_10310"
     CDS             2609..>2827
                     /locus_tag="PLG42_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOU31642.1"
                     /translation="MPYKEKPVEKLFYTIGEVSKILNVPVSTVRFWENQFEILKPRKN
                     KKGNRLFMPEDLKNLKMIHYLLKEKGMTI"
BASE COUNT          912 a          543 c          596 g          776 t
ORIGIN      
        1 cagaggcgac ggaattaaat ttcgcaaaga acatcccgtt cttggtactg caagatggca
       61 ttacggacag gatttccttg caccatatgg tactgaggtg tatgcaacag gagcaggcac
      121 cgttgtagct gcaggctgga gccctaatgg tttcggatat catgtaatca tcgaccacgg
      181 gtatgggttc agaacaatat atgggcacct gagcaagatt gatgtgcctg tgggactaaa
      241 tgtcaaaagg ggtgacagaa taggactgag cggaaattca gggacatctt caggtccaca
      301 cctacattac cagatcgatt ataacggcac acaccagaat cctctatggt tcttctcaga
      361 tgatcttaca cccgaagaat acagagacat gataatgacc ctgaactcaa agtcaaaatt
      421 ccgctgaaaa ataatttttc actgtttttg ataaatttca tcataagtgt ttaaaatcac
      481 acatttacct gattttgttc ttccctttta atttatatat ttgcaatcgc atgggaaaag
      541 caaaatataa gttcaatccg gaatcactga gttttgacag aataacactg ggtttcagag
      601 atattctgtt gagatttatg gcatattttc tcggttcact ttttattgca gcaatttatg
      661 gcatgatttt tacgatattc tttgattccc ccaaagagaa ggccttaaaa cgggaaatag
      721 aacagcttac cctgcaatat gagcttatga accgggaaat ggataacatg gaaaaaattc
      781 tggatcatct taaggaaact gacgataatc tttaccgtac aattttcgaa gcagagcctg
      841 tgccgtcaac tctcagggag ggaggaattg gaggcatcaa ccggtataaa gaacttgaga
      901 actacagtaa tgcaaagcta attatagaga ctgcaaagaa gctcgataga ataaagaaga
      961 aaatagtagt gcagtcaaaa tcgtttgatg agcttatttc actggcacga gaaaaagaag
     1021 aaatgcttgc ttcaatcccg gctataatgc cagtctcgaa caaagacctt accagaactg
     1081 cctctggatg gggacttaga atccatccta tttacaaaat cgtaagattc cactatggca
     1141 tagattttac tgcacctgta ggtactgaag tttatgcaac cggcgatgga gttgtggaaa
     1201 gtatcatttc gtccaggaga ggttacggca atcatattgt tgttaaccac ggttttggtt
     1261 ataagacact ctatgctcac ctcgacagat ttaatgtgcg gcagggacag aaagtaaaaa
     1321 gaggtgatgt tattggtttt gtaggaaaca ctggtctgtc aatagcaccg catctgcact
     1381 atgaggttga acttaacgga ataaaggttg atccatcaaa ttacttcttc catgacctca
     1441 ctcctgagga atacgagcgc atagttgaaa tagcctcacg aagcggacag tcgttcgatt
     1501 aagattatct tattcacagt gcttttatga ggattagcat tatacctttt ttaattattg
     1561 tttgttatca gctttttttt gttttagagg agccaaaagc gcagacaggt gattcctgca
     1621 tgacggcaaa acgttttgtt attgccggcg taggcgatat aatgcccgga acatcttatc
     1681 catcaccgaa atatcttcca ccagataata atccttatcc tctgattgaa aatgtcgccg
     1741 atatcctcag agaaccggat attacattcg ggaacctcga aggctccttc cttaacgaag
     1801 gtgaaccact taaaaaatgc agggatacca caaattgcta tattttcaga atacctgaga
     1861 agtatgccgg tatactctct tatgcaggct tcgatctgat aagcattgcc aataatcatt
     1921 tcatggactt cggatcgccg gcagtaatcc gtacaatgca actacttgat tcccttggaa
     1981 taaaatatgc tggcacagag gaagttccat atgcaataat aaccagggat tcaataactg
     2041 tgggcttctg cgccttttcg gtaaataaag gtactctcaa cctgaacgat tatgaaactg
     2101 cagagaaaat tgtcaggaaa ctcgacaatg agtgcaatat agttattgta tcatttcatg
     2161 gaggttccga aggggcagaa aatcaaagag ttaccagaaa ccgtgaatat ctccacggag
     2221 aagaccgtgg taacgtttat gaatttgccc ataaaatgat agatgcaggt gcagatgtcg
     2281 tattcgggca cggtcctcat gtcacccggg ctatcgaatt atataaagac agattgatat
     2341 gctacagcct tggtaacttt tgcacttacc gtagaataaa cgttgcaggc ccaaacggaa
     2401 tagccccggt agtaagagtc gaaactgaca ttaccggccg gtttatttcg ggtgatataa
     2461 tacccgtata tcatgacgct gaaagaattg tcagatacga tcctttaaaa agggctgttt
     2521 ttaaaatcag agagttaact ttgcttgatt tccctgagac aggcattgaa ataactgatg
     2581 atggtaaaat cataaaaaaa taataaatat gccatacaaa gaaaaaccgg tagaaaagct
     2641 tttctataca ataggagaag tctcaaaaat tcttaatgtg ccggtttcta cggtacgttt
     2701 ctgggaaaac cagttcgaga tactgaaacc ccggaaaaac aaaaaaggaa acagactttt
     2761 tatgcctgaa gaccttaaaa acctgaagat gattcattac ctgcttaaag aaaagggaat
     2821 gacaata
//
LOCUS       DAOSNB010000306         2811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_35760_length_2811_cov_3.745646, whole genome shotgun sequence.
ACCESSION   DAOSNB010000306 DAOSNB010000000
VERSION     DAOSNB010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35760_length_2811_cov_3.745646"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLG42_10315"
     CDS             complement(<1..606)
                     /locus_tag="PLG42_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOU31643.1"
                     /translation="MEFAQFTLKIFNDFVLGNKDYVDLIVNGPYKLTLHSMGLVDADN
                     RVNFYDGKVRVVDTHGNELCKYGPDEYLEYVAEHVEPWSYLKFPYLKKIGWKGFIDGQ
                     DSGVYHATPLSRLNAADGMATPLAQKEYERMYSTLGGKPVHATLAMHWARLVELLYSA
                     ERCLELAMDPDITGPELRAEPGPIVGEGVGIVEAQRGTLTHH"
     gene            complement(534..1181)
                     /locus_tag="PLG42_10320"
     CDS             complement(534..1181)
                     /locus_tag="PLG42_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOU31644.1"
                     /translation="MKRITIDPITRLEGHGKIEIFLDEEGEVKNVFFQVPELRGFEKF
                     CEGRPAEELPSITAKICGVCPGCHHMASGKAVDAVFGLVPPATARKLRELFYMAHFIH
                     SHTAHFYALAAPDFVLGPGAPPEKRNIVGLIERVGKEIGTEVIRQRRIAQEIQALLGG
                     HQTFVVMNIPGGVRRGLSEAERDYVVEKAKGLWNLLSLPLKYSMILYLEIKIMSI"
     gene            complement(1178..2152)
                     /locus_tag="PLG42_10325"
     CDS             complement(1178..2152)
                     /locus_tag="PLG42_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-nonreducing hydrogenase"
                     /protein_id="HOU31645.1"
                     /translation="MEETKPKLKLAVYWAAACGGCCVSVLDVHEKLFDIISAADLVFW
                     PIALDTKYHDVEKMPDGYIDITLFNGAIRNTENEEMAKLLRKKSKILVAYGSCAHLGG
                     IPGLANFTTKDEIFKRVYFESESVVNEKNLIPVPEYKAEEGTLFLPSFYNDVRALSQV
                     TEPDYYLPGCPPQSERLLEVFKAIVSGAELPPKGSVIGAETKTQCDECIRKKTENKRI
                     KKFYRPWEIKDDGITCFMEQGVLCMGPATRAGCGYRCIKGNAPCRGCYGPPPGVPDPG
                     AKMMSAVASIIDEKDPEGINKILEDLADPAGTFYRFSLPVSLIRRKIL"
     gene            complement(2170..2595)
                     /locus_tag="PLG42_10330"
     CDS             complement(2170..2595)
                     /locus_tag="PLG42_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOU31646.1"
                     /translation="MEFKPRIVAFLCNWCSYTGADLAGTSRMEYKPNVRIIRVMCSGR
                     VEPTFVLKAFANGADGVLICGCHPGDCHYQEGNYRCLRRYLLLQKYIRQIGIEPERLK
                     LEWISASEGKQFAELVNSFTEKIKELGPSKIKDTIRTFA"
BASE COUNT          786 a          690 c          494 g          841 t
ORIGIN      
        1 atgatgagtc aatgttcctc tttgggcttc cacaattcca actccttctc cgactatagg
       61 tcccggctca gccctcagtt ccggacctgt tatatcggga tccatagcaa gttcgagaca
      121 gcgttcggct gaatataaaa gttctacaag tcttgcccaa tgcattgcaa gtgtagcatg
      181 aacaggttta cctccgagag ttgaatacat tcgttcgtat tctttctgtg ccagaggagt
      241 agccatccca tctgctgcat tcagtcttga taacggcgtt gcatgatata ctcccgaatc
      301 ctgtccgtcg ataaaacctt tccaccctat ttttttaaga tacggaaact taagataact
      361 ccatggttca acatgttctg ccacatattc gaggtattca tccgggccat atttacataa
      421 ctcatttcca tgggtatcaa caactcttac cttcccatca taaaaattta ccctgttgtc
      481 agcatcaacc agccccatgg aatgaagcgt taacttataa ggcccgttaa ctatcagatc
      541 gacataatct ttatttccaa gtacaaaatc attgaatatt ttaagggtaa actgagcaaa
      601 ttccacaatc cttttgcctt ttcaacaaca tagtctcgtt cagcttccga gagtcctctt
      661 cgtacgcctc cagggatatt catcacaaca aatgtctggt gaccaccgag aagggcctgt
      721 atctcctggg ctattctacg ctgcctaatg acttctgtac ctatctcttt acccactctc
      781 tcaatcagac ccacaatatt tcttttttcg ggtggtgcac caggcccaag gacaaaatca
      841 ggtgccgcca gtgcataaaa atgtgctgta tggctgtgaa taaaatgggc catataaaaa
      901 agttccctca gctttctggc agtagctgga ggaaccagac cgaacaccgc atcaacagcc
      961 ttgccggaag ccatatggtg acaaccggga catacaccgc aaatctttgc tgttatggaa
     1021 ggaagttcct cagcaggcct tccctcacaa aatttctcaa agccccttag ttcaggtacc
     1081 tgaaaaaaca cattctttac ttcaccttct tcatcaagga aaatctcaat ttttccatga
     1141 ccttcaaggc gggttatggg atcaattgta attcttttca taaaattttt cttcttatca
     1201 atgacactgg aaggctaaaa cgataaaaag taccggcagg atctgccaaa tcttcaagaa
     1261 ttttgtttat cccttcaggg tctttctcat caataatgct tgcaacggcc gacatcatct
     1321 tggctcccgg gtcagggacg cccggcggcg gaccataaca tcccctgcat ggagcattac
     1381 cttttataca acggtacccg catcctgccc ttgttgcagg tcccatgcac aaaactccct
     1441 gttccataaa acatgtaata ccatcatcct taatttccca ggggcggtaa aacttcttta
     1501 tacgcttgtt ctcagtcttt ttacgtatac attcatcaca ctgggtcttt gtttcagctc
     1561 caataaccga acctttggga ggcagttccg caccacttac aattgctttg aaaacctcga
     1621 gcaacctttc actctggggt ggacatcccg gtaaatagta atccggttcg gtaacctgcg
     1681 acagagccct tacatcatta taaaacgaag gcaggaaaag tgtaccttct tctgccttgt
     1741 attcaggaac cggaattaag ttcttctcat tcactactga ttcactttca aagtatactc
     1801 ttttaaatat ttcatccttt gttgtaaaat tggcaagtcc gggaatacca cccagatgag
     1861 cacaactccc ataggcaaca agaattttgc tttttttcct taaaagcttt gccatctctt
     1921 cattttcagt gttccttatt gccccattaa aaagtgttat atcaatatat ccatcaggca
     1981 ttttttcaac atcatggtat ttggtatcaa gggctatagg ccagaatacc agatccgcag
     2041 cagagataat atcaaacaac ttttcatgta catcgagaac cgatacacag cagccaccgc
     2101 atgcagctgc ccagtataca gccagtttta actttggttt tgtttcttcc atgggttctt
     2161 gtttttaaat caggcaaatg ttctgattgt atctttaatt ttacttggcc ccagctcttt
     2221 aattttttca gtgaaactat tgaccagctc agcaaactgc ttcccttcac tggcacttat
     2281 ccattccagt ttaaggcgtt caggttcaat tccaatttgt ctgatatact tctgaagcag
     2341 caaatacctg cgaaggcagc ggtaattacc ttcctggtaa tggcagtcgc cagggtggca
     2401 accacatata agcactccgt cagcaccatt tgcaaatgct ttaaggacaa aagtcggctc
     2461 aacccgcccg gaacacatca ctctgattat cctgacattg ggcttatatt ccatccttga
     2521 ggttcctgca agatcagcac cggtgtatga acaccagtta cataaaaacg caactatccg
     2581 tggcttgaat tccatagaaa tagttattag gttagtttaa aaggtacatg cattaaatat
     2641 aaataaaaaa tattcaaagt aagtaattaa actgattttc tttcagttat cgtgactttt
     2701 ttcacaataa ctcattattt tcttccggta gcagaaggga agatttaata tatgctatta
     2761 gctcaacctg gaaacaatca aatttctcct ggatccggta atgtaaagat a
//
LOCUS       DAOSNB010000307         2802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_35920_length_2802_cov_4.830360, whole genome shotgun sequence.
ACCESSION   DAOSNB010000307 DAOSNB010000000
VERSION     DAOSNB010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35920_length_2802_cov_4.830360"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /gene="ilvE"
                     /locus_tag="PLG42_10335"
     CDS             <1..671
                     /gene="ilvE"
                     /locus_tag="PLG42_10335"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOU31647.1"
                     /translation="TGASLYIRPLLIGSGAEVGVKPSPEYIFLVFVTPVGPYFKDGIK
                     PVNMMISRDFDRAAPQGTGTFKVGGNYAASLRALMAAKDSGYATAIFLDAKEKKYIDE
                     CGPANFFGIKNNTYVTPKSESILNSITNMSLIKVAEDMGMKTERRRVPVEELAEFEEA
                     GACGTAAVITPIAKIFDPGTGKVYEFCKDGTPGPVTLKLYKKITGIQYGEEEDPYGWT
                     TVIE"
     gene            738..1616
                     /locus_tag="PLG42_10340"
     CDS             738..1616
                     /locus_tag="PLG42_10340"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOU31648.1"
                     /translation="MNRKDPALTKTRLTGSYITLIISVSLVLFLLGILGLVLINAHGL
                     SKYFRENLSFSVILDEDAREADIRMLQRDLDAKAYVKSTQYISKDQAAAKLKEDLGED
                     FVNFLGYNPLSSTIDVYLYANYTHPDSVKKIEKYILEYPIVKEVYYQESLLYLINENV
                     QKISIFLLVISLFLFLIALTIINNTIRLSIYSKRFLIRTMQLVGATRAFIRKPFLVRS
                     AIHGLIAALIALILLMSLIYLIEEEFFRFISFGNIYLLLVLSGAIIILGIVISVVSSY
                     FSVNKYLSISEDKLYY"
     gene            1621..1872
                     /locus_tag="PLG42_10345"
     CDS             1621..1872
                     /locus_tag="PLG42_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOU31649.1"
                     /translation="MAKRETEKDRGRFALPPDNYKFIAIGFVILVIGFILMLGGKSES
                     PDQFSEKIFSFRRITLAPIIVVAGFVFEIWAIMRKPREK"
     gene            1891..2676
                     /locus_tag="PLG42_10350"
     CDS             1891..2676
                     /locus_tag="PLG42_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOU31650.1"
                     /translation="MNIFNAIILGLIQGLTEFLPVSSSGHLEIAKSIFGLETESSFYF
                     TVAVHGATVMSIIVVFWNEIIKLLKGGLQFKLNDENKYNLKIIVSMIPVVFVGFFFKD
                     TVEAMFTGNLVFVGTMLIVTSVILFAGHFLSMKKSGKGIGYIEAFTIGIAQAVAVIPG
                     ISRSGATISTGLMLGVKKDEVTKFSFLMVLIPIIGANIVELLSGDFVKGEENEAVLIA
                     GFLSAFFSGFLACKWMITLVRKSKMIWFSVYCLLAGIAAIIAG"
BASE COUNT          882 a          429 c          602 g          889 t
ORIGIN      
        1 ggacgggagc atctttatat atcaggcctc ttcttatcgg tagtggtgca gaagtcggtg
       61 ttaagccttc accggaatat atatttctgg tttttgtaac gcctgtagga ccctatttca
      121 aagatggtat taaacctgtt aatatgatga taagcaggga ttttgacagg gctgcaccac
      181 agggaacagg tacttttaag gtaggcggga attatgctgc aagtttaaga gcattaatgg
      241 ctgcaaaaga tagtggatat gcaacagcta tttttcttga tgcaaaagaa aaaaagtata
      301 tagatgaatg tggtccggca aactttttcg ggataaaaaa caatacatac gtaactccaa
      361 aatcagaatc gatactcaac tcaattacaa atatgagtct gattaaggtt gctgaggata
      421 tggggatgaa aacagaaaga aggagagtcc ctgttgagga actcgccgag tttgaagaag
      481 ctggtgcttg cggcacagca gctgtaataa ctccaattgc taaaatcttc gacccgggta
      541 ctggtaaagt ttacgaattc tgtaaggacg gcactccagg acctgtaacc ctgaagcttt
      601 ataaaaaaat cacggggata caatacggag aagaggagga tccatacgga tggaccactg
      661 taattgaatg aaatttattg aaattcgttg ctgtaattca gcaatgttca ctatttttgc
      721 ggcgaaaaaa attcaaagtg aacagaaaag accctgcact gacaaagaca agattaacag
      781 gttcatatat aacacttatt ataagtgttt cacttgttct attcctgctt ggtatactgg
      841 gtctggttct aattaatgcc catggattgt caaagtattt cagggaaaat ctttcttttt
      901 cagtaatact tgatgaggat gcccgtgaag ctgatataag aatgcttcag agagatcttg
      961 atgcaaaggc ttatgtcaag tcgacacaat atatttcaaa agaccaggca gctgccaagc
     1021 ttaaggaaga tcttggtgaa gattttgtta attttctggg atataatccc ctcagctcta
     1081 caatagacgt gtatctttat gcaaattata cacatcccga cagtgttaaa aaaattgaaa
     1141 agtatattct tgaatatcct attgtgaaag aggtttatta tcaggaatca cttctttatc
     1201 ttatcaatga aaatgtacag aagataagta tttttctcct ggttataagt ttatttcttt
     1261 tcctgatagc tctaacaatt attaataata caatcagatt gtcgatatac tcaaagaggt
     1321 tcttaatcag aacaatgcag cttgttggag caaccagggc ttttatacgt aaaccttttc
     1381 ttgtcagaag tgcaattcac ggcctcatag cagcattaat tgcgcttatt cttttgatga
     1441 gtctgatata tcttatcgaa gaagagtttt ttcgttttat ttcattcggg aatatttatc
     1501 tacttcttgt gttgtcagga gcaattataa ttctcggtat agttatttca gttgtctcat
     1561 catatttttc ggtaaacaag tacctgagca tatctgaaga taaattatac tactgaaact
     1621 atggcaaaga gagaaacaga aaaagataga ggaaggtttg cccttccacc tgataattac
     1681 aaatttatag caataggctt tgtaattctt gttattggat ttattcttat gctgggagga
     1741 aaatcggaga gcccggatca gtttagtgaa aaaattttca gtttcagaag aataacactg
     1801 gctcctataa ttgttgtggc cgggtttgtt tttgaaatct gggcaataat gagaaaaccc
     1861 cgtgaaaaat aaataaattc actctttcag atgaatattt ttaatgcaat aatcctggga
     1921 ttaattcagg gtctgacaga atttttacct gttagcagtt caggccacct tgaaatagca
     1981 aaatcaatat tcggtctgga aacagaaagc agtttttatt ttactgttgc agttcacggt
     2041 gctacagtaa tgagcataat tgtggttttc tggaatgaga taataaaatt gcttaaagga
     2101 ggacttcagt ttaagctgaa cgatgaaaat aaatacaatc taaaaattat tgtttcgatg
     2161 ataccggttg tttttgtcgg attttttttc aaagacactg ttgaggccat gtttacgggt
     2221 aatctggttt ttgttggcac aatgcttatt gttacttcag ttattttatt tgcagggcat
     2281 tttttaagca tgaaaaagag tggcaaaggt atcggatata ttgaggcatt tacgattgga
     2341 attgcacagg ccgtggcagt tattccgggt atctcgaggt cgggcgcaac catttcaaca
     2401 ggacttatgc tgggagtaaa gaaggacgag gtaacaaaat tctcatttct aatggtttta
     2461 attccaataa taggagcaaa tattgttgaa ttattgtcag gcgattttgt aaaaggagaa
     2521 gagaatgaag ctgttttgat tgccgggttc ctttctgcct ttttttcagg gtttctggca
     2581 tgcaaatgga tgataactct tgtgagaaag agcaaaatga tctggttttc tgtatattgt
     2641 ttactggcgg gaattgcagc aataattgca ggttaattgg gtgaaggagg aaaaattaaa
     2701 catattcgag gaaggaaaaa ttctgttgat ggataagccg gtttactgga catcatttga
     2761 tctggtgaac aaagtgagaa ttatgctcag ggaaacactt gg
//
LOCUS       DAOSNB010000308         2787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_36160_length_2787_cov_4.076501, whole genome shotgun sequence.
ACCESSION   DAOSNB010000308 DAOSNB010000000
VERSION     DAOSNB010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36160_length_2787_cov_4.076501"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLG42_10355"
     CDS             complement(<1..236)
                     /locus_tag="PLG42_10355"
                     /inference="COORDINATES: protein motif:HMM:NF017689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit A"
                     /protein_id="HOU31651.1"
                     /translation="MAGIYKIKKGFDINLSGKARSVIGGELTAEYYGVRPPDFPGLIP
                     RMSLKPGDRVKAGTTIFTDKKKPEIRFASPVSGIL"
     gene            395..1687
                     /locus_tag="PLG42_10360"
     CDS             395..1687
                     /locus_tag="PLG42_10360"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOU31652.1"
                     /translation="MSYIIKFLAPVLILFSVPIKISAADVSYNDRIFDPRIKTVQLYR
                     EGWNLSYPVLRLNSDEKLVLHFDLLGDNAESYYYTFIHCDKDWNESQIFTTDYLTGFL
                     EDQIEDYRPSFNTKVHYYHYRVMIPNERIGIKLSGNYIIKVYPAGEPGKPAFTKRFMV
                     TEESAAISAAIRRPQPGEFYNTGQQAEINVRLSKITVRDPYNDIFTFILRNGNFNDAK
                     VNLKPDFVAGNEVRYNLLSRENIFYAGNEYRHFDIRSIRYTGENVRKIDYFEGRYHVF
                     LVPSENREFKPYFFMQDFNGKYYVAVQEGRDMDTEADYVYVYFTLPSYSPIEGGDIYV
                     TGAFNNWAFTSENRMTYDPGREQYQCTMLLKQGWYNYEYIYLKHGATSAPPSFFEGNH
                     YETENDYLIFVYYRNPRDRYDRLIGTLITNSSKKEILY"
     gene            complement(1684..2193)
                     /locus_tag="PLG42_10365"
     CDS             complement(1684..2193)
                     /locus_tag="PLG42_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOU31653.1"
                     /translation="MLPELYRILIITLSDRAYNGEYDDRSGPRIEEILREFAMQEKWK
                     LEVERVVLPDDRDMLHRRLAEAVKRFDIIFTTGGTGIGERDITVETVKPFLTKEIPGI
                     MEYIRVKYGAEKPNALLSRGIAGVAGRCLIYTLPGSVRAVEEYMTEILKTLRHTMEML
                     YGIDTHKKH"
     gene            complement(2256..>2787)
                     /locus_tag="PLG42_10370"
     CDS             complement(2256..>2787)
                     /locus_tag="PLG42_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOU31654.1"
                     /translation="IAPDDEDVTYEMVSGAIAENDIVILTGGVSMGDYDFVQDVLKRA
                     GVKILFDKVRVQPGKPTTFGTHQGGVVFALPGNPVSCFVQFEVLVKPFMYKMCGADWK
                     PAVHPMKLAAKYERKSAERAAWVPVRITDDFRAEPVEYHGSAHITAMPYANGLLFMEP
                     GQKIIEKDETVMVRFI"
BASE COUNT          780 a          628 c          554 g          825 t
ORIGIN      
        1 agaattccac ttacaggcga agcaaacctt atctctggct tcttcttgtc ggtaaatatc
       61 gttgtaccag cctttaccct gtcaccgggc ttaagagaca tcctgggaat caatccggga
      121 aaatcaggtg gccttacacc gtaatactcc gctgtaagtt caccccctat aaccgatctg
      181 gcctttccag ataaatttat gtcaaaacct ttttttattt tatatattcc ggccatttgt
      241 ccaattcatt aaaatagaat gaataataac aactctttca aaataatgtt tttaaaaatg
      301 caaatataaa actattcagg tacgcttttt gggatttacc attaatgatt tatattttag
      361 tagtccaaaa taaataaaga tttgaattga aataatgagt tatattatta aatttttagc
      421 tcctgtttta attttatttt cggtaccgat taaaatttca gcggctgatg tttcatacaa
      481 cgatagaatc ttcgacccga gaataaaaac tgttcagctt tatcgtgaag gatggaatct
      541 ctcctatccg gttctgagac tcaacagtga cgaaaaactt gtgctgcact tcgacctgct
      601 gggtgataat gcggaatcgt actattatac ttttatccat tgcgacaaag actggaatga
      661 atcacagata ttcacaactg attatcttac aggtttcctt gaagaccaga ttgaagatta
      721 caggccttcg ttcaatacaa aagttcatta ctaccattac agggtgatga ttccgaacga
      781 aaggataggc ataaaacttt cggggaacta tataataaag gtatatccgg cgggtgagcc
      841 cggcaaacct gccttcacta agaggtttat ggtaacggag gaatcggctg caataagtgc
      901 tgccatacgc aggcctcagc cgggtgaatt ctacaacacc ggacagcagg ctgaaataaa
      961 tgtcagactt tcaaagatta cagtgagaga cccttacaac gacatcttca ccttcatcct
     1021 gagaaatggc aacttcaacg atgcaaaagt taaccttaag cccgattttg ttgcaggtaa
     1081 tgaagtgaga tacaatttgt tatctcgtga aaatatcttc tatgccggaa atgagtaccg
     1141 ccattttgat ataagaagca tccgttatac aggtgaaaac gtgagaaaga ttgactattt
     1201 tgaaggcagg tatcatgttt ttctggttcc ttcagaaaac agggagttca aaccctattt
     1261 cttcatgcag gattttaacg ggaagtatta cgtagctgtt caggagggac gtgatatgga
     1321 cactgaagct gattatgttt atgtgtattt cacacttcct tcgtacagcc cgatagaggg
     1381 tggtgatatc tatgtaacag gagcattcaa caactgggca tttacaagcg agaaccgtat
     1441 gacatacgac cctggcagag aacaatacca gtgcaccatg cttctcaaac agggatggta
     1501 taactatgaa tatatctatc tgaaacatgg ggcaacgagc gctccgcctt catttttcga
     1561 aggcaatcac tacgaaactg aaaatgatta tctgatcttt gtgtattacc gtaatccccg
     1621 cgaccgttac gaccggctca ttggtacgct gataaccaat tcttcgaaaa aagagatttt
     1681 atattaatgt tttttgtgcg tgtcgatgcc atagagcatt tccatggtat gccgtagagt
     1741 cttcaatatt tctgtcatgt attcttctac tgctctcacc gacccgggaa gggtataaat
     1801 caaacacctg cctgccaccc ccgcaatgcc ccggcttagc agagcgttgg gcttttcagc
     1861 accatatttt actcttatgt actccattat acccggtatc tcttttgtaa gaaaaggctt
     1921 tacagtttca actgttatat cgcgttcacc gatgccagtg ccgcctgttg taaagattat
     1981 atcaaacctt ttaacagctt ccgcgagtct cctgtgtagc atatccctgt cgtcgggaag
     2041 aaccacccgc tcaacttcaa gcttccactt ttcctgcatg gcaaactccc tgagaatttc
     2101 ttctatccgc ggaccgctgc ggtcgtcgta ctcaccgttg taggccctgt cggacagtgt
     2161 gataattaat attctgtata actccggaag catattttca actaaacttt taattaatat
     2221 ctatttgttt ttttagaagg gctgtagatg aggtgttaaa taaacctcac catcacagtt
     2281 tcatcttttt cgataatctt ttgacccggt tccatgaaca acagtccgtt tgcataaggc
     2341 atagccgtga tgtgagccga gccgtggtat tcaaccggct cagccctgaa gtcgtctgtt
     2401 attctcaccg gcacccatgc ggcacgctct gccgatttcc tttcatattt tgcagcaagc
     2461 ttcataggat ggacggcggg tttccagtcg gcaccgcaca ttttgtacat aaatggtttc
     2521 acgagaacct caaactgaac gaagcacgag acggggtttc caggcagggc aaacaccaca
     2581 ccgccttggt gtgttccgaa ggtcgttggc ttgcctggct gaactctcac cttatcgaaa
     2641 agaattttta ctccggctct tttcagaaca tcctgcacaa agtcgtagtc gcccatcgag
     2701 acgcctccgg taagtatcac tatgtcgttc tcggcaattg ccccacttac catctcgtat
     2761 gtaacatctt catcatcagg tgcaatt
//
LOCUS       DAOSNB010000309         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_36199_length_2785_cov_3.956777, whole genome shotgun sequence.
ACCESSION   DAOSNB010000309 DAOSNB010000000
VERSION     DAOSNB010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36199_length_2785_cov_3.956777"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLG42_10375"
     CDS             complement(<1..973)
                     /locus_tag="PLG42_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HOU31655.1"
                     /translation="MIKKALILVLVLLLIGSCNFRTEVREKAPAVYVSDLDQLRARGK
                     IIAVTDFNSTNYFIYRGTPMGFHYELLKSFSDYTGINIEVITSGDINKSVELLNSGDA
                     DIIAVDLSMNSERKEQISLTLPLNRSGQVLVQRKPNRWTSMSGEEIDKSLIRDLTSLT
                     GKMIYVQAGSNSALLINRLNRSLGNKFTVIEVPFEAEKIITLVATRKIDYAVCEGNLA
                     QVNARYYPVLDISTVVGEPEELAWGVRKEGSEKLIAALNEWIGTIKSNGLLSILKAKY
                     YSNDNLTRIFKSDYYTLNTGKISPWDLQIKRYSETINWDWRLLASLIY"
     gene            complement(1029..>2785)
                     /locus_tag="PLG42_10380"
     CDS             complement(1029..>2785)
                     /locus_tag="PLG42_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31656.1"
                     /translation="RIADKYSPFNAWMGNYGQPLYQINFFDRITNPDMFLYRYYYPFM
                     YLPSNPVFMNTRVPFTEMVFTYAGPREMADQTFRIRHSQNVNRYLNFGLIYDIIYSLG
                     QYSYQRTDDKSFTFFSSYTGEKYKLYFSSGINSLTTFENGGITDEEQLKTFKTRDVEV
                     RLGALNKARNVLKNSNILLVQRLSLTGKPQKRDSISTPREGIRGLNGTISHIFIWERT
                     RRSYSDSYPLSGFYDTSHIYISRTVTFDSLSERSIKNTLRFDFSTDPERKFRLGGGVG
                     ISNELFRYSQIVPSAENPPSDTVHWTHHNNLLKGSLFNDIGEKFKWRATGELYLTGYR
                     AGDFIADGYIYKVFGQKEKGISLKITGGFSSVKPSVWVFRWGSNHFVWDNNFLKEFRV
                     NAGAEVSYPYHRLNTRFNYTVIDNYTDFGPDSMPAQHRGGLSVAALYAEKELSLWKFH
                     LSNQILIQKSSNRDILDLPLITLRTAGFFEHNFYFKLTGGNLNTQIGVELFYNTKYYG
                     YGYVPATGSYFRQNKTLIGNYPYINAFLNVKIKRTRVFLVLDHINSGFTGYNYFMVPG
                     YPMNVRAFRYGLAWTFYD"
BASE COUNT          830 a          571 c          525 g          859 t
ORIGIN      
        1 gatatattaa cgaagcaaga agtctccagt cccagtttat tgtctcgcta taccttttta
       61 tttgaagatc ccatggtgaa atcttaccgg tgttaagcgt gtaataatcg ctcttaaaaa
      121 tcctggtcag attatcatta ctataatatt tagccttcag tattgaaaga aggccatttg
      181 attttattgt tcctatccat tcattaagag ctgcaataag tttctcagac ccttctttcc
      241 ttacccccca tgcaagttct tcaggttctc ccactactgt agagatgtcc agaacaggat
      301 agtaacgtgc attaacctgt gcaagattcc cctcgcaaac tgcataatca atcttcctgg
      361 tcgcaacaag cgtaattatt ttttcagctt caaacggaac ttctattacg gtgaatttat
      421 taccaagcga acggttaaga cggtttatca aaagtgccga attagaacct gcctgaacat
      481 atatcatttt ccctgtaagt gatgtaagat cccttataag agatttatca atttcttcac
      541 cactcattga agtccagcgg tttggctttc gctgaacaag tacctgcccg gatctgttaa
      601 gcggaagcgt aagacttatc tgctcttttc tttcagaatt cattgaaaga tcaacggcga
      661 tgatatcagc atcacctgaa ttaagcaatt caaccgactt attaatatcg cccgatgtaa
      721 ttacttcaat attaatgcct gtataatcgg aaaacgattt gagcagttcg taatgaaacc
      781 ccataggggt accgcgatat ataaaataat ttgttgaatt aaagtcagtt acagctataa
      841 tctttcctct tgcgcgtagc tgatccaggt cagaaacata aactgcaggg gctttttccc
      901 tgacctctgt tctgaaattg cacgacccaa ttaataaaag aaccagtact aaaattaatg
      961 ccttctttat caatattcta cctttttaat ttatcccttc aaatcaaggc gcaaaggtag
     1021 taattttttt aatcataaaa agtccaggct aatccatatc tgaatgccct gacattcatg
     1081 ggataaccgg gtaccataaa atagttatag cctgtaaagc cggaattgat atggtcaaga
     1141 accaaaaata cgcgggtgcg tttgatcttt acattgagaa aagcattgat gtaagggtaa
     1201 tttccgatga gtgttttatt ttgtctgaaa tatgaacctg ttgccggaac ataaccgtat
     1261 ccgtaatatt ttgtgttata aaaaagttca actccaatct gggtattaag atttccgccc
     1321 gttaatttga aatagaagtt atgttcaaaa aaacctgctg ttcgcaatgt aataagagga
     1381 aggtcgagta tatcacggtt gctggatttt tgaataagta tctggtttga caggtgaaac
     1441 ttccacaacg acaattcttt ttctgcatag agtgcggcaa ccgaaagccc acccctgtgc
     1501 tgagcaggca tggaatccgg accaaaatct gtataattat cgattacggt ataattaaac
     1561 cgtgtattca gccggtggta aggatatgac acctccgcac ctgcatttac tctgaactct
     1621 ttaagaaagt tgttgtccca tacaaaatga ttacttcccc agcggaacac ccatacagaa
     1681 ggttttacag aggaaaaacc gccagtaatc tttaatgata tccctttctc tttctgaccg
     1741 aaaaccttgt agatatatcc atcagcaata aaatctccag ccctgtaacc tgtaagataa
     1801 agttcccctg tagctctcca tttgaatttt tcacctatat cattgaaaag gcttcccttc
     1861 aaaagattat tatggtgggt ccagtgaaca gtatcggaag gcgggttttc ggcagaagga
     1921 acaatctggc tgtatctgaa aagttcattg cttattccaa ctcctcctcc aagtctgaat
     1981 ttcctttccg gatcagtgga aaaatcaaac cttaaggtat tctttatgct gcgttccgac
     2041 agcgaatcga atgttaccgt acggcttata taaatgtgtg aagtatcata aaaccctgaa
     2101 agaggataag aatcagaata tgatcttctg gttctttccc aaataaatat gtgtgaaata
     2161 gttccattaa ggcctctgat tccttcacgt ggagtagaaa tagaatccct tttctgtggt
     2221 ttcccggtta aggacagccg ctggacaaga agaatgttgc tgtttttcag cacatttctg
     2281 gccttattca gggctcccag tcgtacttca acatctcgtg ttttaaatgt tttaagctgt
     2341 tcctcatcgg ttataccacc attctcaaat gtcgtcaaac tgttaatgcc cgacgaaaaa
     2401 tatagtttat acttttcacc tgtatacgac gaaaaaaatg tgaacgactt gtcatcagtt
     2461 ctctggtaac tgtattgtcc gaggctgtaa atgatatcgt aaataagtcc gaagttaagg
     2521 tatctgttta cattctgaga gtgtcttatg cggaatgtct ggtcagccat ctcccttggt
     2581 cctgcatatg taaacaccat ttcggtaaaa ggaacacggg tgttcataaa gacaggattg
     2641 ctcggaaggt acatgaaagg gtaatagtac ctgtaaagga acatatcagg atttgttatc
     2701 ctgtcgaaga aatttatctg gtacaggggc tggccgtaat ttcccatcca tgcgttaaaa
     2761 ggggaatatt tgtcggcaat ccgat
//
LOCUS       DAOSNB010000310         2780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_36286_length_2780_cov_3.238899, whole genome shotgun sequence.
ACCESSION   DAOSNB010000310 DAOSNB010000000
VERSION     DAOSNB010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36286_length_2780_cov_3.238899"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLG42_10385"
     CDS             <1..617
                     /locus_tag="PLG42_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOU31657.1"
                     /translation="IKFFSDDIRPEEKKLTAETSIHYLWFDDTDYEKLGNFIKWNPAI
                     KAQYDREALIRAVNNDQIDIIATDHAPHTVEEKQQPYFKAPSGGPMLQHSLVAMMELC
                     HRKKISAEKIVEKMCHNPSKIFRIKDRGFIREGYKADLCILNPDKPWTVGKENILYKC
                     GWSPFEGVTFQSRVEYTIVNGTIVYNEGIINDNHRGEKLLFNLK"
     gene            624..1142
                     /locus_tag="PLG42_10390"
     CDS             624..1142
                     /locus_tag="PLG42_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31658.1"
                     /translation="MKEKIITLLFAILAVITLNNCRESKKGTEAPSVVTEEISAEAGL
                     LKIGNDIITEVILKPELSGDPWEAEKIKGFNGEQMFLNLLNKIKHDAIKVYDCITGEE
                     IAPKDAKRILQETGSDISRIGKIQFTEDWYFNPSNNMIIKKVKSISFGYETKRNEGLP
                     PAYVPYFQIRPE"
     gene            complement(1151..2422)
                     /locus_tag="PLG42_10395"
     CDS             complement(1151..2422)
                     /locus_tag="PLG42_10395"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOU31659.1"
                     /translation="MKVYKFGGGVVKDAEGVKNIARILSGTDENLIVVVSAFGKTTNA
                     LEKVLETWYNKNGEFRALLGRIYDYHLDIARKLFTDGKEVIDSLNLAFSKLEDYLLTE
                     KIRDYDYEYDQIVSYGEIWASIIVAGYLQKEGFDVEWVDIRNCLITDDNYRDAGILWG
                     ESSKETKRCFDFLKRKIYVTQGFIGGTITGQTTTLGREGSDYTAAVLANMLDAKEVVV
                     WKDVPGILNADPLWLDDAVKLDAISYKEAVEMSFSGAKIIHPKTIKPLHNKNIPLYVK
                     PFFNPGEPGTLVSSISRVTTDAVPVYIRKENQILISVLPVDFSFVMGDNLSIVFHSFM
                     QHRVKVNLVQASAVSINVCVDYEKTKIEKLIKDLKRDYKVLYNDNVEILTVMRYNREA
                     TARILAGREILLEQKTRRVVRYVVRCLKNSI"
BASE COUNT          897 a          599 c          468 g          816 t
ORIGIN      
        1 aaataaaatt cttttcagat gatatacggc ctgaagaaaa aaaacttaca gcagaaactt
       61 caatccacta cctgtggttc gacgatacag attatgaaaa gctcggtaat ttcataaaat
      121 ggaaccctgc aataaaagct caatacgacc gcgaagccct aatcagagcc gttaacaacg
      181 atcagattga tattatcgca accgaccatg caccacatac agtggaagag aaacaacagc
      241 catatttcaa agccccttca ggtggaccta tgctccagca ttcactggtg gcaatgatgg
      301 aactctgcca ccgcaaaaaa atctctgcag agaaaatagt tgaaaaaatg tgccacaacc
      361 cgtcaaaaat ctttagaata aaagaccggg gatttatcag ggaaggttat aaagctgact
      421 tatgtatcct caaccctgat aagccatgga cagtcggtaa ggaaaacatt ctctacaagt
      481 gcggatggtc tcctttcgaa ggtgtgactt ttcagagcag ggttgaatac acaattgtaa
      541 acggtacaat agtatataat gaaggtatta taaatgataa tcacagaggg gaaaaattgc
      601 tttttaacct aaaataaccg gttatgaaag agaaaattat cactttgctg tttgctatcc
      661 ttgctgttat aactttgaat aactgcaggg agagtaaaaa gggcactgag gcaccttccg
      721 ttgttacgga agaaatatcc gccgaagccg gtttgcttaa aataggtaat gatataatta
      781 ccgaagtgat tctgaaacct gaactttcag gagacccatg ggaagctgaa aaaattaaag
      841 gattcaatgg cgaacaaatg tttctgaatc tgctgaataa aataaaacat gatgcaatca
      901 aagtttatga ttgtattaca ggtgaggaaa tcgctccgaa agatgcaaaa aggattcttc
      961 aggaaactgg ttcagatatt tccagaatag gaaaaattca gtttactgaa gactggtact
     1021 ttaatccatc aaacaatatg ataattaaga aagttaaatc catatcattc ggttatgaaa
     1081 ctaaaaggaa tgaaggtctc cctcctgcct atgtccccta ctttcaaata aggcctgaat
     1141 aaccagaatt tcagatagaa ttcttaagac acctcacaac ataccgtaca accctgcgag
     1201 tcttctgctc aagtagaatc tctcttccgg caagaatcct tgccgtggct tcacggttat
     1261 aacgcatcac cgtcagaatc tcaacattat cattgtaaag aaccttataa tctcttttca
     1321 gatctttaat cagtttttca atcttcgtct tttcataatc aacacataca ttaatactta
     1381 ctgcactagc ctgtacaaga ttaactttta ctctgtgttg catgaagctg tgaaagacta
     1441 tactcaggtt atctcccatc acgaatgaaa aatcaaccgg gagaacagaa ataagtatct
     1501 ggttttcctt ccggatgtaa acaggtacag catcagtagt aactctagaa attgacgata
     1561 caagagtccc tggttcgccg gggttaaaga aaggcttcac atatagcggg atgttcttgt
     1621 tatgaagggg ttttatggtt ttagggtgaa ttatttttgc acctgaaaac gacatttcaa
     1681 cagcctcctt atatgaaatc gcgtcaagct ttacagcatc atcgagccag agaggatcgg
     1741 catttaaaat acccggcaca tctttccaga ccacaacctc ttttgcatcg agcatattag
     1801 ccagaactgc agctgtataa tcagaacctt cacgtccgag tgtggttgtc tggcctgtaa
     1861 ttgtacctcc tataaatccc tgagttacat aaatttttct tttcaaaaaa tcaaaacatc
     1921 tttttgtttc cttactgctt tctccccata atatacctgc atcccggtaa ttatcatctg
     1981 taattaaaca atttcttata tcaacccatt caacatcaaa accttccttc tgcaaataac
     2041 ctgcaacgat tattgaagcc catatttcgc cataagaaac tatctgatca tattcataat
     2101 cgtaatctct tattttttct gttaacagat aatcctcaag ctttgaaaag gcaagattta
     2161 acgaatcaat aacctccttt ccatcagtaa acaacttcct ggcaatatcc agatgataat
     2221 cataaattct tcctagaagt gccctgaatt caccattctt gttataccag gtttcaagca
     2281 ctttttcaag agcatttgtt gttttcccaa aagcagaaac caccacaatc aggttttcat
     2341 ctgttcccga tagtatccgg gcaatatttt tcacaccctc cgcatcttta accactcccc
     2401 ctccgaattt gtaaactttc ataggtaaat aattttataa tttttcccta atcgtaaccc
     2461 ttattttgcc aaggaatagg ttattgtgaa gcctcccgaa aaaagttgac atcactttat
     2521 aaatatacaa tctctcactt ttaattcata tagacacccc ctttttcttt catttttcta
     2581 taatgatccc tttttatttt ctttaagcat aaactgacca cctttgtatt tcctttaagc
     2641 ataaactgac ccccctttgt atttcccccc acggggggaa agatttaaga cagaattcta
     2701 tgtatcaatt tgttaagctt ttcccccctg gggcccgtct gactggcgtc agccgggcag
     2761 ggaaacggga aagggggtca
//
LOCUS       DAOSNB010000311         2779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_36300_length_2779_cov_3.335536, whole genome shotgun sequence.
ACCESSION   DAOSNB010000311 DAOSNB010000000
VERSION     DAOSNB010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36300_length_2779_cov_3.335536"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..859
                     /locus_tag="PLG42_10400"
     CDS             182..859
                     /locus_tag="PLG42_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HOU31660.1"
                     /translation="MANEEIQKQIDEINRKLDLLLEDASVRKQNQEALNDLADDISLI
                     SKEAFKYLVNDLDNAGVELDSDALRCLIIKSIRNLANLGRMIDTIESINDLLLDVEPI
                     LRQIGLDGIRKFNEIEQKGYFDLINQFAVSADKIMSRYSREEINKISDNIVIIADIII
                     NLTNKQTLKKAHAMSVALKELDLNNIQEISVWKLLFRLNRPEVKKSIGFLMAFLEKLN
                     NTETEIN"
     gene            888..1199
                     /locus_tag="PLG42_10405"
     CDS             888..1199
                     /locus_tag="PLG42_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOU31661.1"
                     /translation="MAQKVYAGVTVDVNEEGYFTNPSQWTREIAAEIAKEEGIDLTEQ
                     HYQVIEWLRKRYESGEALSIRSINKSGIIDVKTFYQLFPGAPLKKATKIAGIPKPASC
                     V"
     gene            1218..1718
                     /locus_tag="PLG42_10410"
     CDS             1218..1718
                     /locus_tag="PLG42_10410"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOU31662.1"
                     /translation="MANDKDKIKKVNIICAKGAIEDVYATLVLGNGAVMEGIETNIFF
                     TFFGLDAIVKSRMDKLHTAVVGNPALRLPGGMRMPTLLGVIPGMEAFTSWMMKKEMEK
                     LDIPPVSEFMELITAGGGKIYACKLAMDMFKFKKEDLCEYVSGILTVGEFYELAGGEG
                     SHIIFT"
     gene            complement(1711..>2779)
                     /locus_tag="PLG42_10415"
     CDS             complement(1711..>2779)
                     /locus_tag="PLG42_10415"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOU31663.1"
                     /translation="RLTGVSRAGKRERGSINKRNGKTYHFSVNFFQDDMQTLYLKVMI
                     LKDFTIFTYMIPDEIKIKPVKPVLSGYISEALRLLKNETFPDEDSVHDIRVLMKKARA
                     AVKLVAHQIDSEFFEREYMTFREAGRLLAEWRETSVHRKTLKSIRKSHEDLFSRLSGY
                     PFLESIIATPLEFSSPGKAESDRIEKIEEILRKSLYRVRFQSFGEQDFRLMFQALEST
                     YNEVAGIYLECRRKPRPGKIHSLRKRAKDLLYQLWFFRPMNPPVIKRIEKRLDSLTRD
                     IGKYNDLEQLLQVIGYEPGNPENPPDINELLIIIRDKQHNYLSDVWSEAYRLFCPGQK
                     LVNLLGFRLLSVDLPSGAKIR"
BASE COUNT          907 a          536 c          552 g          784 t
ORIGIN      
        1 gggcttcttc cctttcccgg gcattggacc attcagttta ttgaaagaaa gcaggataaa
       61 ccactatggc aaactcatat tcagatggat gtactggcat atattactga aaggtaaaga
      121 gatgcctatt gataccaata tgaccatggc tggcaaaaaa accgattaaa acattatggg
      181 aatggccaac gaagaaatac agaaacaaat agatgagatc aacaggaaac tggatcttct
      241 cctggaagat gcctctgttc gcaaacaaaa ccaggaggca ctgaatgacc ttgctgatga
      301 catctcactc attagcaaag aagcttttaa gtatcttgta aatgatcttg ataatgcagg
      361 tgtggagctg gatagtgatg cattaagatg ccttataatc aaatcaataa gaaacctggc
      421 caacctggga agaatgatcg atacgataga aagcatcaac gaccttctcc ttgatgtcga
      481 accaatttta aggcagatcg gactagatgg gataagaaaa tttaatgaga tagagcaaaa
      541 aggttatttt gatcttatta accagtttgc agtatcggcc gacaaaatta tgagccgtta
      601 ttcaagagag gaaataaaca aaatatcgga taatattgtt attatagcag acatcataat
      661 aaatttaacc aataaacaga cattgaaaaa ggcacatgcc atgtcagttg ctcttaaaga
      721 attggattta aacaatatac aggaaatctc cgtatggaaa cttctcttca ggctgaacag
      781 gccagaagtt aaaaagagca taggatttct tatggcattt cttgaaaaac tgaataatac
      841 tgaaactgaa ataaattaat atttaattaa catcaaaaca aaaagttatg gcacaaaaag
      901 tatatgctgg agtaacagtt gacgtaaacg aagaaggata ttttaccaat ccttctcaat
      961 ggacaagaga aattgctgct gagattgcta aagaagaggg tattgacctt actgagcagc
     1021 attatcaggt tattgaatgg cttagaaaaa gatacgaaag tggtgaagcg ttatcgataa
     1081 gaagcattaa taaatcgggt attattgatg taaaaacatt ttatcagctt tttcccggag
     1141 ctcccctgaa aaaggctaca aaaattgcag ggattcccaa acctgccagt tgtgtttaat
     1201 tattaaaatt caatatcatg gcaaatgata aggataaaat aaagaaagta aacatcatct
     1261 gcgcaaaagg cgcaattgag gatgtttatg caacacttgt gcttggtaac ggtgcagtaa
     1321 tggaaggtat cgaaactaac atttttttca cattcttcgg actcgatgcc attgttaaaa
     1381 gcaggatgga taaacttcac acagctgtag ttggcaatcc tgccctccga ttacccggtg
     1441 gcatgagaat gccgacactt cttggtgtaa tacccggaat ggaagcattc acgtcatgga
     1501 tgatgaagaa ggaaatggaa aaactcgata ttcccccggt aagcgagttt atggaactta
     1561 tcactgctgg aggaggtaag atatacgcct gcaaacttgc aatggatatg ttcaagttta
     1621 aaaaagaaga tctttgcgaa tacgtatcag gaatacttac tgtgggagag ttctacgaac
     1681 ttgctggcgg tgaaggttca catataatat ttacctgatc tttgcccctg aaggcaaatc
     1741 aaccgacagc agcctgaaac caagaaggtt taccagcttt tgacccggac agaacaacct
     1801 gtaagcttca gaccatacgt ctgataggta gttatgttgt ttatccctta taataatcag
     1861 aagctcattt atatcgggag ggttctcagg gttgcctggt tcatatccga taacctgcaa
     1921 taattgctcc agatcattgt atttgccaat gtcacgtgta agactgtcaa gtcgtttttc
     1981 aattcttttt ataactggtg gattcatcgg tctgaaaaac cagagctggt ataataaatc
     2041 cttggccctc tttcttaaag agtggatttt accaggccgt ggcttcctgc ggcattccag
     2101 atatatccct gcaacctcgt tataggttga ttcaagtgcc tgaaacatca gcctgaaatc
     2161 ctgttcaccg aaactttgaa agcgcacccg gtaaagcgac ttccttaaaa tctcttcaat
     2221 tttttcaatt ctatcagatt cagccttacc aggtgatgaa aactcaagag gtgtcgctat
     2281 aatactttca agaaaaggat accctgaaag ccggctaaaa agatcttcgt gtgatttcct
     2341 gattgactta agagtctttc tgtgaactga tgtctccctc cattctgcaa gcaaccgtcc
     2401 ggcttccctg aaagtcatat attctctttc aaagaattca ctatctatct gatgagcaac
     2461 aagtttcaca gcggctcttg ccttcttcat caataccctg atgtcatgaa cagaatcctc
     2521 atccggaaag gtttcattct taagtaaccg cagtgcttcc gaaatgtatc ccgacagcac
     2581 aggctttacc ggttttattt ttatctcatc aggtatcata tatgtaaata tagtgaaatc
     2641 ttttaaaatc ataaccttta aatagagtgt ctgcatatcg tcctgaaaaa agttgacaga
     2701 gaaatgatat gttttcccgt ttcttttatt aattgacccc ctttcccgtt tccctgcccg
     2761 gctgacgcca gtcagacgg
//
LOCUS       DAOSNB010000312         2728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_37225_length_2728_cov_3.322110, whole genome shotgun sequence.
ACCESSION   DAOSNB010000312 DAOSNB010000000
VERSION     DAOSNB010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37225_length_2728_cov_3.322110"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLG42_10420"
     CDS             complement(<1..440)
                     /locus_tag="PLG42_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31664.1"
                     /translation="MRCCILYNQPRENALADELDILDQVEWVETNLKKAGIETYRKGI
                     TNNFMNEISLLADEKPDFVFNLVESINNKGELLYFIPALLNMHSIPYTGNPLEALFIT
                     TSKFLTVKLLKNEGIRTPESFHISEYKKFEPGKSYIIKPVWEDGS"
     gene            complement(437..1414)
                     /locus_tag="PLG42_10425"
     CDS             complement(437..1414)
                     /locus_tag="PLG42_10425"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31665.1"
                     /translation="MKAGITFDLRSWYLERGFSMEDTAEFDKEATISAIRNVLESMGF
                     DTEEVGNVFQLIEALASGKRWDIVFNIAEGLYGDGRESVVPAILDQYRIPYVFSGPVV
                     LGVALNKYLAKLVASSAGVPVSPGFLITSLDDIKKCDLEYPLFIKPASEGTGKGITNK
                     SILKKPAELKEVAEYLLKRYNQPALVEEYLPGREFTIGILGTGDEAVAIGGMEVICKD
                     ELPYSVEYKENYHLYCNYKPLDDSDISKESKDVALRAWKAIGCADGGRVDVKADRHGR
                     ICFMEVNPLAGLHPIDSDLPILARFSGIEYNELVERIIRSALKRHNLKL"
     gene            complement(1421..2662)
                     /locus_tag="PLG42_10430"
     CDS             complement(1421..2662)
                     /locus_tag="PLG42_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOU31666.1"
                     /translation="MNANNNEASAFHGEGRLKGCSSDEEPGAKAVILSQVETPVQIVS
                     NRNADFIHRYFPLATNEQWNDWKWQLRNAITTLEDLRKIMPLTEKEIMAIKHLKGRLP
                     LRITPYFASLIHNTKPGNPLRRIVIPVVEELIQLPVEKPDPLHEEAYSPVKGIVHRYP
                     DRVLFTVTQVCSAYCRYCTRSHSVGKLDKLGKNDFEKAFDYIRNHTEIRDVLVSGGDP
                     LTMSDEMLDYILKNIRNIPHVEIIRIGTRVPVVLPQRITDKLIATMRKYHPLYISLHF
                     SHPDEITDECAQACNKLADGGFPLGSQTVLLKGINDNVEIMKKLFHKLLMIRVRPYYL
                     YQCDLIPGSGHFRTSIRKGLEIIKGLRGYTSGYAIPQFVVDAPGGGGKIPLLPDYVVA
                     HTKEKIVLRNYKGMICEYPEI"
BASE COUNT          714 a          582 c          549 g          883 t
ORIGIN      
        1 gaaccgtcct cccatacagg tttgattata tagcttttgc caggctcaaa ttttttatat
       61 tccgagatat gaaaagattc tggtgttctt ataccttcgt ttttaagtag tttaactgta
      121 agaaatttac ttgttgtaat aaacagagcc tccagcgggt tgcctgtata gggtattgaa
      181 tgcatgttaa gcagggcagg aatgaaatat agtagttctc ccttgttatt tatagactca
      241 acaagattaa aaacaaaatc tggtttctca tcagccagaa gtgatatttc attcatgaaa
      301 ttattcgtga taccttttct gtaagtttca attccggctt ttttaagatt ggtttcgacc
      361 cattcaacct ggtcgagaat atcgagttcg tcggcaagag cattttcgcg aggctgattg
      421 taaagtatgc agcatctcat aacttcagat tatgtctttt taatgcagac ctgatgattc
      481 tttcaaccag ttcgttgtat tcgatacctg agaaccgtgc aagtattggc aggtctgaat
      541 caatcgggtg aagtccggcc agagggttaa cctccataaa acatattctg ccatgtctgt
      601 cagcctttac atccacacgt ccgccatcgg cacagccaat tgctttccat gctctcagag
      661 ccacatcttt tgattccttt gaaatatcac tatcgtcaag gggtttgtaa ttacagtata
      721 aatgatagtt ttccttgtat tcaactgaat atggaagttc atctttacag attacttcca
      781 ttccccctat tgccaccgcc tcgtccccgg tgccaagaat tccaattgtg aattcccggc
      841 ctggaagata ttcttcaaca agtgccggct gattgtaccg tttaagcagg tattctgcca
      901 cttctttcag ttctgcaggt tttttaagga tactcttatt tgttatgcct ttaccggtac
      961 cttcagaggc aggtttgata aaaagtgggt attcaaggtc gcacttttta atatcgtcaa
     1021 gggaggtaat cagaaagcca ggactaaccg gcacaccggc tgatgaagct accagttttg
     1081 ccagatattt atttagagca actccaagta ctaccggtcc gctgaaaaca tatggtatac
     1141 ggtattgatc gagtatggcg ggaacaactg actcccgtcc gtcaccatac aatccttcgg
     1201 caatattaaa gacgatatcc catcttttac ctgatgcaag ggcttcaata agttgaaaga
     1261 cgttaccaac ttcttcggta tcaaaaccca ttgattcaag cacatttctt atagcgctta
     1321 ttgtggcttc tttatcaaat tctgcagtat cttccataga aaacccgcgt tcgaggtacc
     1381 acgaacgcag gtcaaaagta atccctgcct tcataaaaaa ttaaatttca gggtattcgc
     1441 aaatcatacc cttatagttg cggagaacta ttttttcctt tgtgtgtgct acgacataat
     1501 cgggcaggag tggtatttta cctcctccac ccggtgcatc cacaacaaat tgtggaatgg
     1561 cataaccgct tgtatatcct ctcagtcctt tgattatttc cagtcctttc cttattgatg
     1621 tccggaaatg tcctgaaccc ggaataaggt cgcactggta cagataataa ggcctcactc
     1681 ttatcataag caatttatgg aagagttttt tcataatttc cacattatca tttatgcctt
     1741 taagcagtac agtctgactg ccgagtggaa aaccaccatc agccagtttg ttgcatgcct
     1801 gtgcacactc atctgttatt tcatccggat gtgaaaaatg caggctgata tagagtgggt
     1861 ggtattttct catggtggct ataagtttat ccgttattct ctgtggcaga accacaggca
     1921 cccttgtacc tatcctgata atctctacat gggggatatt tcgaatattc ttcagaatat
     1981 aatcgagcat ttcatctgac atagtaagtg gatctcctcc acttacgaga acatctctaa
     2041 tctcggtatg atttcggata taatcaaagg ctttttcaaa atcattcttt cccagcttgt
     2101 cgagtttgcc taccgaatgt gagcgagtgc agtacctgca ataggcagag caaacctgag
     2161 tcacagtaaa caggactctg tcgggatatc tgtggacaat tccttttaca ggtgaatatg
     2221 cttcttcgtg cagtggatcg ggcttttcaa caggcagttg aataagttct tcaactacag
     2281 gtattacaat tctcctgagt ggattgccag gtttggtgtt gtggataaga gatgcgaaat
     2341 aaggcgtaat tcttaatggc aatctgcctt tgagatgttt gattgccata atctctttct
     2401 ctgttaatgg cattattttt ctgaggtctt ccagcgtagt aatagcattc ctcagttgcc
     2461 atttccagtc gttccattgt tcattagttg cgagagggaa gtagcggtgg atgaagtccg
     2521 cattccggtt gctaactatt tgtactggcg tttcgacctg cgaaagtatt actgctttcg
     2581 cgccaggctc ttcatcagaa gagcaacctt tgaggcgacc ttctccatgg aaggctgaag
     2641 cctcattatt gttagcattc atgatgttgt gaaaatttca ctcctttttt agttgtctgt
     2701 ttcttgttta aaattttgcc gaaagtat
//
LOCUS       DAOSNB010000313         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_37613_length_2706_cov_4.652207, whole genome shotgun sequence.
ACCESSION   DAOSNB010000313 DAOSNB010000000
VERSION     DAOSNB010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37613_length_2706_cov_4.652207"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..553)
                     /gene="rsmG"
                     /locus_tag="PLG42_10435"
     CDS             complement(32..553)
                     /gene="rsmG"
                     /locus_tag="PLG42_10435"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOU31667.1"
                     /translation="MIRDTLTKYFPSLSGVQLEKLCRLEGLYSEWNSKINVISRRDIE
                     NFNIHHLLHSLSIARVISFVPGTEILDIGTGGGLPGIPLAIMFPEVHFVLLDSIRKKI
                     KVVTEIAAALQLTNVKPVWERAENHRGSYDFIVSRAVADFPELVRIAEGKIKKESRNE
                     LKNGIIGSVQNFV"
     gene            complement(550..1167)
                     /locus_tag="PLG42_10440"
     CDS             complement(550..1167)
                     /locus_tag="PLG42_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOU31668.1"
                     /translation="MEIDEVIRDTSELSEKARQDLILVEQAKNGDEKAFAALLNRYRD
                     SIYYMLLKMVNNSSDAKDLTIEAFSKAFQSINTYTPDFAFSTWLFKIATNNCIDFLRK
                     KHKTPQVLEQTDEDDDEGYEARIESDSPDPEEIMINNQDIDNLHKVVEQLKPKYRKLI
                     ELRYFREYSYEEISEELKIPIGTVKVQLFRAKAILYELLSRKGKK"
     gene            complement(1158..2231)
                     /locus_tag="PLG42_10445"
     CDS             complement(1158..2231)
                     /locus_tag="PLG42_10445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOU31669.1"
                     /translation="MSVQLFYYLWFYLATLKYRHPETDGNLPPVSVIICARNEAENLK
                     KFLPLVLEQDYPSYEVIVVNDCSEDDSYKILGEFLAKYPHLKVSTLTKDPLFPHSKKF
                     AQFIGIKAASHDILLFTDADCYPVTNQWIRTMVSGFQPGREFVLGYGGYIKSKGLLNI
                     YQRYETLFIAMQYMGMAIKGIPYMGVGRNMAYLKEVFIRNKGYGSYTHTISGDDDLFV
                     NENAKPSTVSVEFRPAAHTRSVPSSSLTEWVKRKLRHLSTARFYKPVHKILLVAEPFT
                     RIIFYTLFIYLMLTGFFWQIVLGLFILRFLVTHTIFFLNAKKFEEKGILPLFLIFDIF
                     SPVINLILYSGTIRSKSHSMPWK"
     gene            2457..>2706
                     /locus_tag="PLG42_10450"
     CDS             2457..>2706
                     /locus_tag="PLG42_10450"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HOU31670.1"
                     /translation="MFTVTANDRDSKARAGILKTSHGDVPTPVFMPVGTAGTVKGVLH
                     RDLINDIGAGIILGNTYHLYLRPGMEILKAAGGLHRFMS"
BASE COUNT          777 a          553 c          534 g          842 t
ORIGIN      
        1 gttggtgtcc caaatgcagc tgccttttca tttacacaaa attctggaca ctaccaataa
       61 taccgttctt caattcattt ctactttctt tttttatttt tccttcggca attcttacca
      121 gttcagggaa atctgccacg gcacggctga caataaaatc gtagctgcct ctgtgattct
      181 cggctcgctc ccacacgggc ttaacatttg tgagctgcaa tgcggcagca atttccgtca
      241 cgacttttat ttttttcctt atcgaatcga gtaaaacaaa atgcacctca gggaacatta
      301 ttgcaagcgg aatacctggt aggcctccgc cggttccgat atcgagaatt tctgtcccgg
      361 gaacaaaact tattactctg gcaatcgaca gtgaatgaag cagatgatga atattgaaat
      421 tttcaatatc cctgcggctt atcacattta tcttactgtt ccactccgaa tataacccct
      481 ccagcctgca tagtttttcc agttgcacac ctgaaagaga aggaaaatat tttgtgagag
      541 tatcccttat cattttttcc ccttccgcga gagtaactca taaagaattg cctttgccct
      601 gaaaagctga accttcacag ttcctatagg gattttcagt tcttcagata tctcttcata
      661 ggaatattcc ctgaaatatc tcagttctat aagctttctg tatttgggtt tgagctgttc
      721 tacaacctta tgcagattgt caatatcctg gttgttaatc attatttcct cagggtctgg
      781 agaatctgat tcaatgcggg cttcataccc ttcgtcgtca tcttcatctg tctgttccaa
      841 tacctgaggg gttttgtgct ttttcctgag gaagtcaata caattgtttg ttgcaatttt
      901 gaagagccat gtactgaatg cgaaatctgg agtatatgta ttaatgctct ggaatgcttt
      961 actgaatgcc tcaatagtaa ggtctttggc atcggaggaa ttgttgacca tcttcagcaa
     1021 catgtaatat attgagtcgc ggtacctgtt cagaagagct gcaaaagcct tctcatcacc
     1081 gtttttcgcc tgctccacca ggatgagatc ctgccgggcc ttctccgaaa gctcagaagt
     1141 gtcccttatt acttcgtcta tttccatggc atactatggc ttttacttct gatggtaccc
     1201 gaatacaata ttaaatttat taccggtgaa aatatatcaa aaatcagaaa gagaggtaaa
     1261 atacctttct cttcaaactt tttcgcatta aggaaaaata tagtatgagt caccagaaat
     1321 cttaaaataa acagacccag aactatttgc caaaagaaac ctgttaacat aagatagatg
     1381 aacagggtat aaaaaattat tcttgtaaat ggttcagcaa cgagcaggat tttgtgaaca
     1441 ggtttgtaga atcttgcagt gctgaggtgt cttagttttc ttttaaccca ttcggtgagg
     1501 cttgaagatg gtacagatcg tgtatgggct gcaggcctga attccacact tactgttgaa
     1561 ggtttagcat tttcgtttac aaaaagatca tcgtcgcccg atatggtatg ggtgtaacta
     1621 ccataccctt tattcctgat gaaaacctct ttcaaataag ccatattgcg cccaactccc
     1681 atatacggta tcccctttat tgccattccc atgtactgca tggcaataaa aagagtttca
     1741 tatctctggt aaatgttaag aagtcctttt gatttaatat atcccccata gcccagaaca
     1801 aactccctgc ctggttgaaa tcctgaaacc attgttctaa tccattgatt agtaacaggg
     1861 tagcagtcgg catcggtaaa aagcagaata tcatgtgacg cagctttaat accaataaac
     1921 tgagcaaatt tttttgagtg agggaagaga ggatcttttg taagtgtgga gactttcaga
     1981 tgcgggtatt ttgccagaaa ttcaccgagt atcttgtaag aatcatcttc tgaacagtca
     2041 tttacaacga taacttcata cgacggataa tcctgttcaa gtaccagagg aaggaatttt
     2101 ttaagatttt cagcttcgtt tcttgcacag ataattaccg atacgggcgg aaggtttcca
     2161 tcagtttcag gatgcctgta ttttagtgtt gcaagataaa accatagata ataaaaaagc
     2221 tgaactgaca ctgctgtgca gtatataaca aatagagtca gaattaactg actgaaggat
     2281 tggtgcatct gttttataga tatcaggagc gaaaataaga atataattat ttactcattt
     2341 agtttttttt caacattaga cggttaacta ataacagcct gcagcaaaaa agaatcggat
     2401 atttttcaga gaggcaacat ataaatatta cctttgtgct cctgaaagca cagaagatgt
     2461 ttaccgtaac tgctaacgac agggatagca aggcaagggc cgggatattg aagacatcac
     2521 acggggatgt gcctactccg gtatttatgc ctgtcggtac tgcaggcacg gtgaaaggtg
     2581 ttcttcaccg tgacctcatt aatgatattg gtgcagggat aattcttggg aacacttatc
     2641 acctgtatct tcgtccgggt atggagatac tcaaagctgc gggaggtctt caccggttta
     2701 tgagct
//
LOCUS       DAOSNB010000314         2705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_37647_length_2705_cov_5.553962, whole genome shotgun sequence.
ACCESSION   DAOSNB010000314 DAOSNB010000000
VERSION     DAOSNB010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37647_length_2705_cov_5.553962"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..499)
                     /gene="rplU"
                     /locus_tag="PLG42_10455"
                     /pseudo
     CDS             complement(200..499)
                     /gene="rplU"
                     /locus_tag="PLG42_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            816..1415
                     /locus_tag="PLG42_10460"
     CDS             816..1415
                     /locus_tag="PLG42_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOU31671.1"
                     /translation="MKRIKLKVLGISYSQTQSGAYALVLMEEKGQRRIPIIIGAFEAQ
                     SIVIKLENLEPPRPLTHDLFKNFADTFGIEITEVFIHKLEDGIFFSKLICYKDDEEYI
                     IDSRTSDAVALALRFNCPIYTTEEIMEKAGITIPQSEGGTQDFTADKTIKETEASIFE
                     GYTDEELYNMIDEAVKTEDYERAAAIRDELEKRKKRKSK"
     gene            1444..>2705
                     /locus_tag="PLG42_10465"
     CDS             1444..>2705
                     /locus_tag="PLG42_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 N-terminal
                     domain-containing protein"
                     /protein_id="HOU31672.1"
                     /translation="MKKNIIIILLLLVYCNYLFARDFKISSPDGNISVTISTGKQITW
                     SVTYSGNEILASPRTAIVLGDGTIPVENQSIRRPVTGRINEILRPEVPHKRSTITDNC
                     NYLSIVFRSGFTLTFRVYNDGVAYRFESDLKDSLIIQNEIAELSFPDGTTSWFPYESG
                     FMSHNENTFVYSLLDTITSKHLASLPVLFKPGNINVLITESAIEEYPGMWLRGQGNGR
                     LTGVWPPYPAEERLSGDRNLRVTKTEDYIAKVTGKRVYPWRVFIITPDDGGLVESDMV
                     YRLAAPCRLKDTGWIKPGKVAWDWWNALNLYGVDFKAGINNETYKYYIDFAARYGLEY
                     VILDEGWYRQGNVLDVIPGLDIPELCRYGAEKNVGIILWVVFKALDDRMDEALAKFAE
                     WGVKGIKVDFFEKESKTACTFSINLFSKP"
BASE COUNT          768 a          546 c          578 g          813 t
ORIGIN      
        1 gggttgcttc tgtttcttcc tctttttcgg cggtttctgc ctttttagtc cttccagttt
       61 ttttaaccac ttcagcagct tctgcaggag cagtttcggc tttcactgtg gtatcctcaa
      121 caggtgcaac ctcctttggt ttttcagatg cctcttctgc cttctttgct gccgactttc
      181 tcggagcttt ctcctttatg cttactattt caacctgcgt gaaatactgg cgatgtccgt
      241 tcttaaccct gtaacccttt cttctcttct ttttgaatac tataaccttg tcgcctttca
      301 gatgctcaag aaccttggct tctacgactg cgtctttaat aacaggttct cctacagtta
      361 ccttgccatt gtcctcaatc agaaggacct ggtcaaattc aactttattt ccgttttctg
      421 ccttaagccg gtggacaaat atcttttttc cttcttccac cttaaactgc tgcccggcaa
      481 tctctacaat agcatacata gtttttaatc tattttgtta ttccgtgagg cgtaccgttt
      541 ttacgtcggt atctttacaa gccctgccgg atttaattta ttaattcgga ctgcaaagat
      601 ataaaatttc aaatatatgc aagctatttt cttatctttt tatttttatg ttatatcgta
      661 tagcacctta acaatgagaa gtttctgatt atgcagtcta ataatgatta taaagttaat
      721 aaaaaatgac cattgaagaa gggtattgtg ctctataatc tttatatttg ttgtgatacg
      781 tagtttgatt agtttcgtac agcagaacgt ggtttatgaa gaggataaag ttaaaagttc
      841 tggggatatc atacagtcag acacagtcgg gtgcctatgc gctggttctg atggaagaaa
      901 agggacagag gaggattcca attattattg gcgcattcga agcacagtca attgtaataa
      961 aacttgagaa cctggaacca ccgcgtcccc ttactcatga tctttttaaa aattttgcgg
     1021 ataccttcgg gattgaaata actgaggttt ttattcacaa gctagaagac ggcattttct
     1081 tctcaaagct catatgttac aaggacgatg aggagtatat tatcgacagc aggacctcag
     1141 atgcagtggc acttgccctc aggtttaact gtccgatata tacaaccgag gaaataatgg
     1201 agaaagcagg gatcacaatt ccgcaatcgg aaggcggaac ccaggatttt acggctgaca
     1261 agactataaa ggagacagaa gcttcaatat tcgaaggata tacagatgag gaactctata
     1321 acatgattga cgaagcagtc aaaacagaag attatgaacg ggctgccgca ataagagatg
     1381 aactcgagaa gagaaagaaa cgaaaatcga aataatttac gtcttttata aattcaataa
     1441 cttatgaaga aaaacataat tattattctt ctgctacttg tctattgcaa ttaccttttc
     1501 gcgagggatt tcaagataag ttcacccgat ggaaacatat ctgtaacaat cagtaccgga
     1561 aaacagatta cctggtcggt tacatactcc ggtaatgaaa tcctggcatc gccacgtaca
     1621 gccattgttt tgggcgatgg aaccattccg gtggagaacc agagcatcag aaggccggtg
     1681 acagggcgaa ttaatgagat tttacgccca gaagttcccc acaagaggtc aactatcacc
     1741 gacaactgca attatctgtc aattgtcttc agatcgggtt ttacgcttac attcagagtt
     1801 tataacgacg gagtcgctta caggtttgaa tcggacctga aagattcgct cataatacag
     1861 aatgaaattg cggaactctc ttttcctgac ggaacaactt cgtggtttcc ctatgagtca
     1921 ggcttcatgt cgcacaacga aaacactttt gtttattctc tccttgatac tataacctca
     1981 aaacatcttg caagcctgcc ggttcttttc aagccaggca acataaatgt tctgattact
     2041 gaatcggcta ttgaagaata ccccgggatg tggcttcgtg ggcagggtaa tggccggtta
     2101 actggcgtct ggccccccta tcctgcagaa gaaaggcttt cgggcgaccg taatctgaga
     2161 gtgacaaaaa cagaagatta tattgcaaag gttacaggca aaagagtgta tccctggagg
     2221 gttttcatta ttactcccga tgatggtggg cttgttgaaa gtgacatggt ataccgcctg
     2281 gcagctccct gcagattaaa agataccggc tggataaaac ccggcaaggt ggcatgggac
     2341 tggtggaatg ccttaaatct gtacggcgtt gatttcaagg ctggcatcaa caatgagaca
     2401 tataaatatt atattgattt tgctgccagg tacggattgg aatatgttat actcgacgaa
     2461 ggatggtacc ggcaggggaa tgtgctcgat gtgatacccg gccttgatat cccggaacta
     2521 tgcagatacg gggctgaaaa aaatgttggc attatactct gggtggtgtt taaagcgctg
     2581 gatgacagaa tggatgaagc cctcgcaaaa tttgcagagt ggggcgtaaa aggaataaag
     2641 gttgattttt ttgaaaaaga atcaaagaca gcttgtactt tttctataaa tttattttca
     2701 aaacc
//
LOCUS       DAOSNB010000315         2683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_38029_length_2683_cov_4.420472, whole genome shotgun sequence.
ACCESSION   DAOSNB010000315 DAOSNB010000000
VERSION     DAOSNB010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38029_length_2683_cov_4.420472"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLG42_10470"
     CDS             <1..840
                     /locus_tag="PLG42_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOU31673.1"
                     /translation="RCPECVTGSGQSKRPAGFMEVELYKKILSELKSYLLNVNLYFQG
                     EPMLHPDFFKFVSLSRGIRLTLSTNGHYLTDENISRIIESDIREIIVSLDGYDRQTYS
                     AYRRGGDFEKVREGILKLAAALKEKDSRTKLVVQFLVNRHNEQQIQEVRNFAVGAGAR
                     FHLKSMQIINPKGFEEWLPSHEKFRRYFKDDNGDYKLKSRLKNRCFRLWLSPVIAWNG
                     DVLPCCFDKNAEFVLGNIGESSFREIWHGERNRAFREKVLHNRAGIDICRNCTTGLRG
                     VII"
     gene            890..>2683
                     /locus_tag="PLG42_10475"
     CDS             890..>2683
                     /locus_tag="PLG42_10475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU31674.1"
                     /translation="MRTLRIILLSFLFLILAALVAVLIVVRNISRGALPSYQGEMQLS
                     GLIDEVTVYRDERGIPHIYAANEHDLYFATGYIMAQERLWQMDLIRRVTTGRLSEIFG
                     KDYIKTDLFLRSLNMTEKSEKVISNTETEIMECLQAYVDGVNCYIHERGRKLPPEFRI
                     LSYTPEPWSVIDMTNIIGYMGWDLASDNLYSDLFNYRLVKKMGEQKASSLIPDWKAPC
                     TTVFPDFKLDDNSALALMKLISYTDKITSSVFGPFSGSNNWAVSGSKTETGKPVMCND
                     MHLSFGSPGIWIQMHQVIPGKINVTGVLIPGEPFVVAGHNEKIAWGLTNLMVDDIDLY
                     EEKINPSDTNQYFFNGEWVNMKVKKELIRIKGGSDTAMIIRFTHRGPIISEFENVKDA
                     KLSMRWSGYDESNEIKAVYLLNKASNFDEFRNAISHFRSISQNFVYADIEGNIGLNTG
                     GGIPLRKGPGTIIRDGQSGEWDWKGYVPFAQLPFVYNPPEGHVSSANNKTVEDYPVYI
                     SSEFALPYRINRIRQMLNEKEVMTIEDFKRMITDQHSDYAARLTPEILKVVALRMDSL
                     NETERKAFASLQKWDYDMNKDLVAPSVFEFFR"
BASE COUNT          854 a          457 c          635 g          737 t
ORIGIN      
        1 agatgccctg aatgcgtcac gggatcaggg caaagtaaaa ggccagcagg atttatggaa
       61 gtagaacttt acaaaaaaat tttaagtgaa ttaaagtctt atttgttgaa tgtcaatctc
      121 tactttcagg gcgagcctat gctccacccc gatttcttca aattcgtttc gctttccaga
      181 ggcatcaggc ttaccctctc aacaaatgga cattatttga cggatgagaa tatcagtagg
      241 ataattgaat cggatataag agaaataatt gtttcactcg acggatatga caggcaaacc
      301 tattctgcct accgcagggg tggagatttt gaaaaagtca gggaggggat attaaaactt
      361 gcagcagctt tgaaagagaa ggactcccgg acaaaacttg tagtgcagtt tcttgttaac
      421 aggcacaatg agcaacaaat tcaggaagtc agaaattttg ccgtgggagc aggtgccaga
      481 tttcacctga aatcgatgca gataattaat cccaaaggat ttgaagagtg gctgccatcg
      541 catgagaaat tcagaaggta tttcaaagat gataatggag actacaaact taagagccgg
      601 cttaaaaaca ggtgcttcag gttatggctt agtccggtta tagcatggaa cggtgatgtt
      661 ttgccctgct gttttgacaa aaatgccgaa tttgttctag gaaacattgg tgaatcttct
      721 ttcagggaga tctggcacgg agaaaggaac agagcgttca gggaaaaagt gctccacaac
      781 agggcaggca ttgatatttg cagaaattgt accaccggac tcaggggagt aattatttaa
      841 atattccgga taaaattgta aattgaataa aaatttaatt ttcatatata tgagaacttt
      901 aagaatcatt ttgttatcct ttctcttcct gatactggcc gcacttgttg ctgttctaat
      961 tgttgtcaga aacatcagca gaggagcttt accttcatat cagggggaga tgcagctaag
     1021 tgggttgatt gatgaggtta ctgtataccg cgatgagcgc ggcatacctc acatatacgc
     1081 tgcaaatgaa cacgatctct attttgccac aggctatatt atggctcagg agagactctg
     1141 gcagatggat cttatacgaa gggtgacaac aggccgtctt tcagaaatat tcggaaaaga
     1201 ctatatcaag acagaccttt ttctccgtag ccttaatatg actgaaaaat cagaaaaagt
     1261 aatcagtaac acggagactg agataatgga gtgccttcag gcctatgtgg atggtgtgaa
     1321 ctgctatatt catgaaagag gcaggaaatt acccccggag ttcagaatac tttcatatac
     1381 accggagcca tggtcggtta ttgatatgac aaacataata ggttacatgg gctgggatct
     1441 ggcttccgac aatctctatt cagatctttt taattaccgt ctggtaaaga aaatgggaga
     1501 acaaaaggct tcatctctga tacccgactg gaaggcaccc tgtacaacgg tcttccccga
     1561 cttcaagctt gatgacaatt ctgctttggc actcatgaaa cttatttcat atacagataa
     1621 aataacatct tctgtttttg gtccattttc aggaagcaac aactgggctg tctcagggag
     1681 taaaacagaa acaggaaaac ctgtaatgtg caatgatatg cacctgagtt ttggctctcc
     1741 tggaatatgg atacagatgc atcaggtaat tcctggtaaa ataaatgtaa caggagttct
     1801 tattccggga gaaccttttg tggtggcagg acacaatgaa aaaattgctt gggggttaac
     1861 taaccttatg gttgatgata ttgatctgta tgaagaaaag attaatccgt ccgatactaa
     1921 ccagtatttc tttaatggcg aatgggtcaa tatgaaggtg aaaaaggagc tcatcagaat
     1981 aaagggagga agtgatactg caatgattat cagatttact catcgtggac caataatatc
     2041 ggaatttgag aatgtgaaag atgcaaagct ttccatgaga tggtcgggat atgatgaaag
     2101 taacgagata aaggctgtat atctattgaa taaggcttca aattttgatg aattcagaaa
     2161 tgcgataagc catttcagat cgataagcca gaattttgtc tatgccgata ttgaaggtaa
     2221 tatagggttg aataccgggg gaggtattcc tttaagaaag ggtccaggga cgataatcag
     2281 ggatgggcag agcggtgaat gggattggaa aggatatgta ccttttgcac agttgccttt
     2341 tgtttataac ccgcctgaag gtcatgtgtc atcagcaaat aataaaacag tggaggatta
     2401 tccggtttat ataagttctg agtttgcact accataccgg attaacagaa tcaggcagat
     2461 gctgaatgaa aaagaagtga tgacaattga agatttcaag aggatgatta cagatcagca
     2521 ttccgactat gcggccaggt taactccgga gatattgaag gtagtggcat taagaatgga
     2581 ttcactgaat gagactgaga ggaaggcatt tgcatcactg cagaaatggg attatgatat
     2641 gaataaagat ctggtggctc catcggtttt cgaattcttc aga
//
LOCUS       DAOSNB010000316         2653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_38593_length_2653_cov_3.395689, whole genome shotgun sequence.
ACCESSION   DAOSNB010000316 DAOSNB010000000
VERSION     DAOSNB010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38593_length_2653_cov_3.395689"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PLG42_10480"
     CDS             <1..1081
                     /locus_tag="PLG42_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31675.1"
                     /translation="VFKLTDKYNNVTTSEISITRKKVEEKIIRPEYKHIIARKQAELF
                     IDMLKKRADGKLKGVIEKSDFRKQQFGTPDDILAFIKEEAAKQNISGEEVDRLALKVA
                     VMDNILTQAAVDIMARYTTGKLKEILDNLNIYDENLKTFTDLQRFIEEKTGGAIAPEQ
                     LKKIAEEIIAGIDPMIAIIREQIKRYGEISGKSEPVNNAIAAVDSKNILKSGLWLKDF
                     FYEALNKKLSEQDLAAMFKALTALPGTHIEDYIKEFIGYAKGGFADYLKSLNLRKNRI
                     DTPEELINFLIRQRNMNLFQEDELFETIAEFIAKKKIEKEKIIAPEAEKKNYLFILWI
                     ILGAGLIFFFIILMRRRKKKDEREE"
     gene            1231..2508
                     /locus_tag="PLG42_10485"
     CDS             1231..2508
                     /locus_tag="PLG42_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31676.1"
                     /translation="MKKIFTLIIAFLGFFVFSANSQDLKAMQEQFLEAEYFFVSGFYN
                     DALPIYLNLYEQMPDNANLAYRIGVCCLNINGKKKLSVEYLESAVKNVSSRRREGTIM
                     HTMAPYDALFQLGNAYRVNYQFDKAKEAYQKYRRTLLKADVENISFVDHQIEVCNNAR
                     NLISNPARHIIEPASIPLSEEKDNFDPVVSADGNTFVYMTVLPFYNAVMITTRKEDKW
                     SEPENITQWLQPEGQIFVSSLSADGKTLFFSKSDPFNNDIYSITRESSGWSAPVKLNK
                     NINTKYWESQAYITEDGNTIVFSSDRPGGYGGLDIYISYRENGDWGLAKNLGPTINTS
                     FNEDRPALVDGGKVLFFTSQGHENIGGYDIFRSVKTQKGTWSNPENLGYPVNTPDDNI
                     FYTPANNGTAAYISLYREPEGARESIYYVIFKF"
BASE COUNT          932 a          447 c          523 g          751 t
ORIGIN      
        1 cgtattcaaa cttactgata aatacaataa tgtaacaact tctgaaatat ccatcacaag
       61 gaagaaagta gaagagaaaa ttataagacc tgaatacaaa catattattg ccaggaagca
      121 ggctgagtta ttcattgata tgcttaagaa aagagcagat ggtaaactta aaggggtaat
      181 cgaaaagagt gatttcagaa agcagcaatt cggaactcca gacgatattc tcgcatttat
      241 taaggaagag gctgcaaaac aaaatatcag tggtgaagag gttgaccggc ttgcccttaa
      301 ggtggccgtt atggataata tccttaccca ggctgccgtg gatattatgg cacgctatac
      361 aacaggaaaa ctaaaagaaa ttcttgacaa tctaaatatt tacgatgaaa acctgaaaac
      421 atttacagat cttcagaggt ttattgaaga aaagaccggt ggagcaattg caccagaaca
      481 gcttaagaag attgcagaag aaataattgc aggcatcgat ccgatgattg caattataag
      541 ggaacagata aagagatacg gtgagatttc aggcaagagt gaacctgtta ataatgccat
      601 agctgctgtt gattcaaaga atattctgaa atcaggactc tggctcaaag acttcttcta
      661 tgaagcactc aacaaaaaac tttctgaaca ggatcttgca gcaatgttca aagcattgac
      721 tgctttaccc ggtacccata tcgaggatta tattaaagaa tttatcggtt atgcaaaggg
      781 tggatttgct gattatctta agagcctcaa cctgaggaaa aacagaattg atacccctga
      841 agaactgata aatttcctca tccggcagag gaatatgaat ctcttccagg aagatgaact
      901 ttttgaaact atcgctgaat tcattgcaaa gaaaaaaata gaaaaggaaa aaattattgc
      961 acctgaagcg gaaaagaaaa attatctgtt tatcctctgg ataattcttg gagccggact
     1021 aatattcttc ttcataatct taatgagaag aagaaaaaag aaggatgaaa gagaagaata
     1081 aatctatatt cattgaaaga ggggctttaa tcaggaagtc cctcttttaa tttaataaga
     1141 tcatgaaata aagtctgttg ataattttga ttcattaaca atcgtttata taaaacagtt
     1201 tttttacttt tactgaagaa ataaggaaaa atgaagaaaa ttttcacatt aataattgct
     1261 tttcttggct tttttgtttt ttcagccaat agtcaggact tgaaagcaat gcaggagcag
     1321 tttcttgaag ctgagtattt ctttgtgtcg ggtttctata atgatgcttt acccatctac
     1381 cttaatcttt atgaacagat gccagataat gccaacctgg cataccggat aggagtctgt
     1441 tgcctgaata ttaatggtaa aaagaaatta tcggtagaat atttagaatc agcagtaaag
     1501 aacgtttctt caaggcgcag ggaaggaaca attatgcata caatggcacc gtatgatgca
     1561 ttgttccagc ttggcaatgc ttaccgggta aactatcagt ttgacaaagc aaaggaagca
     1621 tatcaaaaat acagaaggac gctccttaaa gctgacgtag agaatatcag ttttgttgat
     1681 catcagatag aagtatgtaa taatgccaga aatcttattt caaaccctgc aaggcatata
     1741 atcgagcctg ccagtatacc tttatctgaa gaaaaagaca atttcgaccc tgttgtttca
     1801 gctgacggaa acacatttgt ttacatgaca gtactgcctt tttataatgc agtaatgatt
     1861 acaacaagga aagaagacaa atggagtgaa cctgagaata tcactcaatg gttgcaacct
     1921 gaaggccaga tatttgtctc ctcactgtcg gcagacggaa agacactttt cttctctaaa
     1981 agtgatcctt ttaacaacga catatattca atcacccgtg aaagcagcgg atggtctgca
     2041 ccggtaaaac tgaataaaaa tatcaataca aaatactggg aatcacaagc atacataact
     2101 gaagacggaa atacaattgt attttcctct gacagacccg gaggatatgg cggacttgat
     2161 atttatatct cctatagaga aaatggcgac tggggcctgg cgaaaaatct tggtccaaca
     2221 ataaacacca gtttcaatga agacagacct gctcttgttg acgggggcaa agttctgttt
     2281 tttacttcac agggacatga aaatatagga ggatacgaca ttttcaggtc ggttaagaca
     2341 cagaagggaa cgtggtcaaa tcctgaaaac ctcggctatc ctgttaatac tccagatgac
     2401 aatatctttt acacaccggc aaataatgga acagcagcat atatatcatt gtacagggaa
     2461 ccggagggtg ccagggaaag tatttattat gtgattttta aattttagcg agagataatg
     2521 aattttcatt tggaatctac ccgttttttt ctatatttgc ttaaaatgtt tattatcagg
     2581 atgaaaaatt ttatccggtt atttatttcc ctgatattca ttattatatc agctgttgtt
     2641 aattcccagt cgt
//
LOCUS       DAOSNB010000317         2652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_38611_length_2652_cov_3.398152, whole genome shotgun sequence.
ACCESSION   DAOSNB010000317 DAOSNB010000000
VERSION     DAOSNB010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38611_length_2652_cov_3.398152"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLG42_10490"
     CDS             complement(<1..420)
                     /locus_tag="PLG42_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31677.1"
                     /translation="MLRLKIKLALFNLVSKMVVAGLFIVFLPYITQRINLRQIDNSLI
                     QKREKVISLIEEYGIEPFIEADSANAFGSYNILKEEFISIEHFDNEEEINSIEVAERL
                     IENEILKYRVHNYSIKVDGEPYLIEIGKSLESISQTER"
     gene            complement(420..1112)
                     /locus_tag="PLG42_10495"
     CDS             complement(420..1112)
                     /locus_tag="PLG42_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOU31678.1"
                     /translation="MNVLIVEDEKSLAREIASFLEKENFLCEIAHTGRDASEKIAVNL
                     YDFILLDLGLPDYDGLELIKEAKKLNSEASFIIITARGAVEDKVKGLNLGADDYLAKP
                     FALVELLSRINAVARRKFNVKTDDIALGEFTLKVLSRKLYYKEKEVELTRKEFDLLQY
                     LALNRNKVLTRQQLYEHIWGNILDDQYDSNFIDVHVKNLRKKLNEYAPAPWLETVRGV
                     GYRITLEDQSKR"
     gene            complement(1125..2003)
                     /locus_tag="PLG42_10500"
     CDS             complement(1125..2003)
                     /locus_tag="PLG42_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31679.1"
                     /translation="MKAVTIFPVLIIAAFPHISSAQETVAEASAADTSSNGGRKQGIH
                     SLYAGTGYGSNMVYLGSTISQDKPYGYASLTYGIGDKFYLSGSAVHLTGTIPYVAFYT
                     ASASFSHTFNSWLDISAGLSRYQVHKSLTDTLFESFTYGEISLGIDWKVLYTKLSAGR
                     IFSTESSSYYQIRNSRYFQTPEFGSGKFYFSFDPYVNLILGTLTKTETSNSSSIVFTP
                     PFRKRGMNQQTSGSTTYKSIFGILEADFGIPVAFNSSRLTIEAEPGYIYPLYEESAYP
                     GTKGFLFLLSAYLRIF"
     gene            complement(2014..2421)
                     /locus_tag="PLG42_10505"
     CDS             complement(2014..2421)
                     /locus_tag="PLG42_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31680.1"
                     /translation="MIKKRDINKMKIKTLILTIPVLFVFSLVNVYSQENVAGTDTTRQ
                     KKTEVATVKKEQNQAQVQNRVQERNQVQGAENAGQQAKPQGQNTGKQVKQVTSARPDM
                     SKARGARPPQITRPSGPPKGIGKPGGAPGRGGR"
BASE COUNT          681 a          571 c          581 g          819 t
ORIGIN      
        1 acgctctgtc tgtgatatgc tttcaaggct tttgcctatt tcaatgagat agggttctcc
       61 gtcaaccttt atggaataat tgtgaacacg gtattttaat atttcattct cgattaatct
      121 ttcggcaacc tctatgctat ttatttcttc ttcattatca aaatgttcta tgcttatgaa
      181 ttcttcctta agaatgttat aactaccgaa ggcatttgct gaatcggctt ctatgaaagg
      241 ttcaatgcca tattcctcga ttaaagaaat aactttttca cgtttctgaa tcagactgtt
      301 gtcgatctgc ctgaggttta tacgctgggt gatataagga agaaagacaa taaacagacc
      361 tgccacaacc atttttgaga caaggttgaa cagggcaagt tttattttta atctcagcat
      421 taccgtttcg actgatcttc aagagttatt ctgtagccca ctcctctgac agtttcgagc
      481 catggggcag gggcatattc attgagtttc tttctaaggt ttttaacatg gacatcaata
      541 aaattgctat cgtactggtc gtcgagaata tttccccaga tatgttcgta aagctgttgc
      601 cgggtaagaa ccttgtttcg gttcaaagcc agatactgaa gcaggtcgaa ttcttttctg
      661 gtcagctcga cttccttttc tttataatag agtttgcgcg acagtacttt aagagtgaat
      721 tctccaagag caatgtcatc tgttttaaca ttgaatttcc tccgggcaac agcatttatt
      781 cttgagagaa gttcgacaag tgcaaagggt tttgccaggt agtcgtcggc accgaggttg
      841 agacctttta ctttgtcttc cacggcaccc cgggcggtaa ttatgatgaa tgatgcttca
      901 ctgtttagtt ttttggcttc ttttataagt tcgagtccgt cgtaatctgg aagaccgagg
      961 tcgagtagta tgaagtcgta aaggttaact gcaatttttt ccgaagcatc tctccccgta
     1021 tgggcaattt cacacaggaa gttctctttc tcgagaaacg atgctatttc acgtgcaaga
     1081 cttttttcat cctcaacaat caggacattc atttgttttt tatattaaaa tattctaaga
     1141 taagcgctta aaaggaaaag aaaacctttt gttccagggt atgctgattc ttcgtataaa
     1201 ggatatatgt aaccaggttc agcttcgatt gttagtcttg acgagttgaa agcgacaggt
     1261 attccgaaat cagcttctaa aatgccgaag atacttttgt aggttgttga acctgatgtt
     1321 tgctgattca tgccccgttt acggaaagga ggcgtaaaca ctatgcttga ggagtttgag
     1381 gtttcagttt tcgtaagtgt gcccagaata agatttacat acggatcaaa tgaaaagtaa
     1441 aactttcctg aaccgaattc gggtgtctgg aaatatcttg aattccttat ctggtaatag
     1501 ctgctgctct cagttgagaa aatcctgccg gcagatagtt ttgtatagag gaccttccag
     1561 tcgatgccaa gggagatctc tccatatgta aaactttcaa agagggtatc ggtaagcgac
     1621 ttatgcacct ggtaacgtga taagcctgct gagatatcga gccaggagtt gaaagtatgg
     1681 ctgaagctgg ctgaagcagt atagaaagca acataaggaa tagtacccgt cagatggaca
     1741 gctgaaccac tgagataaaa tttgtcgccg ataccgtatg taagtgatgc atatccgtaa
     1801 ggtttatcct gagaaattgt tgaacccaga taaaccatgt tgctgccgta accggtacct
     1861 gcatagaggg aatggatgcc ctgctttctc ccgccgtttg atgaagtatc ggcagcggag
     1921 gcttcagcta ctgtttcctg agcggatgag atatgaggaa aagcagctat gatgagtact
     1981 ggaaagatgg ttacagcttt catattatct tatttatctc cctcctcttc ccggtgctcc
     2041 tccgggcttt ccaatccctt tgggcggacc tgatggtctt gtaatctgag gaggtctggc
     2101 accccttgcc ttgctcatat cgggccgggc actggttacc tgcttaacct gctttccggt
     2161 attctgtccc tgaggtttag cctgctgacc tgcattttca gccccctgaa cctgatttct
     2221 ctcctgaaca cggttctgaa cctgcgcctg attctgttct ttcttcaccg tagctacctc
     2281 tgtttttttc tgtcgcgtgg tatcagtgcc agcaacattt tcctgggaat aaacatttac
     2341 aagggaaaaa acaaacagaa caggtattgt taatataagt gttttgattt tcattttatt
     2401 tatatctcgt ttcttaatca tctctgcagc aaatttatga attcaaaatg aagaaacgat
     2461 gaagaagaga tgatttgtat ttactcaccc cccgaccccc ctgggagggg ggcacccccc
     2521 cgacccccct gggaggtagg acccccccaa cccccctgag aggggggcac ccccccgacc
     2581 cccctgggag gggggcaccc cccgaccccc ctgggagggg ggcttgacat tgaataaata
     2641 tttatcttta tc
//
LOCUS       DAOSNB010000318         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_39319_length_2614_cov_3.092224, whole genome shotgun sequence.
ACCESSION   DAOSNB010000318 DAOSNB010000000
VERSION     DAOSNB010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39319_length_2614_cov_3.092224"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1479
                     /locus_tag="PLG42_10510"
     CDS             358..1479
                     /locus_tag="PLG42_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOU31681.1"
                     /translation="MGKESLSVPKRLQSLDALRGFDMFWIMGGEAIFAGLATLTGWPV
                     FKWFAEQLEHVQWHGFHFYDMIFPLFLFIAGISFPFSLSKRMASGEDKARIFRHVIYR
                     GLILVLLGILYNNGVRFKFDELRYGSVLGRIGLAWMFAALIFMNTKLTWRIVWFWALL
                     IGYWLLLALVPAPDGGGAHVFSQEGNFASYVDRILMPGKLYRGNHDPEGILSTVPAIS
                     TALLGMFTGEFLLSNYLSNNLLKKAIYIAGAGIVFVIVGKVWDLAFPINKNLWTSSFV
                     CYVGGLSLLLFALFYLIIDVWNFRKWSFFFVVIGMNPITIYLANRIINFRNAAKFFFG
                     GFTEILPETWTPLINGIGITAVGWVFLYFLYKKKIFLKI"
     gene            1508..2563
                     /locus_tag="PLG42_10515"
     CDS             1508..2563
                     /locus_tag="PLG42_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOU31682.1"
                     /translation="MRDSKRHRATHLKVALFCLTAMLQAASCGQGINELNNESKGLKD
                     YYQNYFPVGVAVAPSHLNPSAPERNLIIREFNSLTAENVMKPGLIHPEENRYFWDNAD
                     IIINFAGEHGMKVRGHTLVWHQQTAPWMFRNSDGTEASKELLLSRLKEHITQVVTRYK
                     GKVYAWDVVNEAIDDSEGKMYRESPWYRICGEEYIEKAFIWAHEADPQALLFYNDYNT
                     ENPAKRDKIYNMLKGLLEKGIPVHGIGLQGHWSISSPSEKELREAIEKYSSLGLQVQI
                     TELDVSVYRWNEDFSNRKPGDDAFTAEREQRQIEQYKMLFRVFRDYKGIITGVTFWNV
                     SDKHSWLDNFPVRNRKN"
BASE COUNT          785 a          525 c          566 g          738 t
ORIGIN      
        1 tgggttgaaa accctgtggt ggagatttcc ctgcctcctg ccccgacttc ggctgatgac
       61 tttattgccc agttttcttt aagcaccatc accggacctt cagggtgctg ggcaggtgct
      121 gataaggtta agaataacat gactgttgtt aagacgatta tgatcttttt catggcttaa
      181 tgatttaatt ccaggaattg atcgtaaaca ataaattttc ctattataaa aataaataaa
      241 tcctttattg cagcaaaata ccggtttgaa aaattccgaa aggaaatcca aagttttaaa
      301 ttttcaaact atctttcgag gagttaatat ttaacattca tattataaac aaaaaatatg
      361 ggaaaagaat ctttatctgt accaaagcga cttcagtcac tcgacgcttt acgcggattt
      421 gacatgttct ggataatggg cggtgaagct atttttgcag gacttgccac tttaaccggc
      481 tggccggtct ttaaatggtt tgccgaacaa cttgaacatg tccagtggca tggttttcac
      541 ttttacgaca tgatattccc gctttttctg ttcatcgcag gaatctcatt ccctttctca
      601 ctctcaaaaa gaatggcctc tggagaagat aaagccagaa ttttcaggca cgttatctac
      661 cgcggtctca tactcgtact gctgggcatc ctttacaata acggagtaag gttcaaattt
      721 gatgagctcc gctatggaag cgttcttgga cgcatcggac ttgcatggat gttcgcggct
      781 ctaatcttta tgaacacaaa acttacatgg cgtatagttt ggttctgggc acttcttatc
      841 ggctactggc tgctacttgc cctggtgccg gcacccgacg gaggaggagc tcatgtattc
      901 tcacaggaag gcaacttcgc aagttatgtc gacagaatac tgatgcccgg aaaactgtac
      961 cgcggtaatc acgaccctga aggaatcctc agtaccgtcc cggcaatatc aacagctctg
     1021 ctcggtatgt ttactggaga atttctgctg tcgaactatc tgagcaataa cctgctcaaa
     1081 aaggcaattt atattgccgg tgcaggaata gtattcgtta ttgttggcaa agtatgggat
     1141 cttgctttcc caattaacaa gaatttgtgg acaagctcct tcgtatgcta tgtgggcgga
     1201 ctgagcctgt tgctgtttgc actgttttac ctgataattg acgtctggaa tttcagaaaa
     1261 tggtcattct tcttcgttgt gataggtatg aacccaataa caatatacct tgctaaccgc
     1321 ataataaatt tcagaaatgc tgcaaaattt ttctttggcg gatttactga aatactacct
     1381 gagacatgga ctccacttat aaacggtata ggtataactg cagttggatg ggtgtttctt
     1441 tatttccttt ataaaaagaa aatattttta aaaatctgaa aaatataaaa cggtaattac
     1501 ttaaaccatg cgagactcaa aaagacacag agctactcat cttaaagtgg cattattttg
     1561 cctgactgct atgctacagg ctgcaagctg tggccagggc atcaatgaac ttaataacga
     1621 aagtaaagga ttaaaagact attaccagaa ttacttccct gtaggtgtag cagttgctcc
     1681 ctcgcacctg aatccttcag cacctgaaag aaatctgata atcagagagt tcaatagctt
     1741 gactgcggaa aatgtaatga aacccggatt gatacatccg gaagagaacc gttatttctg
     1801 ggacaacgcc gacattataa taaattttgc tggcgaacat ggcatgaagg ttagaggtca
     1861 tactcttgtt tggcatcagc agactgctcc ctggatgttc agaaattccg acggcacgga
     1921 agcttcaaaa gagttactcc tttcaaggct taaagaacat attacacagg tagttaccag
     1981 atacaaaggc aaagtctatg catgggatgt agttaacgaa gcaatagatg acagcgaggg
     2041 gaagatgtac agggaatccc cctggtacag aatatgcggt gaagagtata tcgaaaaggc
     2101 ctttatctgg gcacatgaag ccgaccctca ggctcttctc ttttacaacg actataatac
     2161 cgaaaaccct gcaaaacgag ataaaattta taacatgctt aaaggtttgc tcgaaaaggg
     2221 tattccggtg cacggaatcg ggctgcaggg gcactggtca atttcaagcc catcagagaa
     2281 agaactacga gaagctattg aaaaatattc atcactcggg cttcaggtcc agattactga
     2341 gcttgatgta tctgtttaca ggtggaatga agatttcagc aaccggaaac caggggatga
     2401 tgcttttact gctgagaggg agcagagaca aattgaacag tacaaaatgc ttttcagggt
     2461 tttcagggat tataaaggca taattacagg tgtaactttc tggaatgttt cggacaaaca
     2521 ctcatggctc gacaatttcc cggtacgaaa caggaaaaac taacctcttc tgtttgacca
     2581 gaatttgcaa aggaagaaag catattacga ggta
//
LOCUS       DAOSNB010000319         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_40000_length_2580_cov_5.464554, whole genome shotgun sequence.
ACCESSION   DAOSNB010000319 DAOSNB010000000
VERSION     DAOSNB010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40000_length_2580_cov_5.464554"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PLG42_10520"
     CDS             <1..1235
                     /locus_tag="PLG42_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOU31683.1"
                     /translation="TAWMLTSTALVLLMVPGLALFYGGLVRSKNVLGTMMHSFAAMGI
                     ITVLWMVAGYSLSFGRSVFGGFFGWNNDYFFLKGIDSNILDAGIPEYVFTMFQGKFAI
                     ITPALIAGAFAERISFKAYCFFIAIWSLLVYDPLCHWVWASDGFLFNLGAKGAIDFAG
                     GTVVHISSGVSGLVAAIYLGSRRGYPYQVIRPNNLVITMLGAGLLWVGWFGFNAGSSI
                     SSGLSTAQALTATQIAAASGALTWIVIEGIHKGKTTALGFASGILAGLVAVTPAAGVV
                     QPYGALFIGLFATLFCYTAIQIKNKLGYDDSLDVFGIHGVGGITGALLLTFFIRPSWM
                     ADAAKVSHTGSWTIWNQLGVQSIAVGVAIAYAAIMTLVIIYVLNKVMKIRSDEEDEMA
                     GLDRSYHGERGYGMVNPS"
     gene            1259..1606
                     /locus_tag="PLG42_10525"
     CDS             1259..1606
                     /locus_tag="PLG42_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOU31684.1"
                     /translation="MKLITAIIREVQLDKVREALIEAGINRITVSLVSGNGRQRIEEM
                     YRGLTIIPDLIPKIRIEIAVNDESVEKAIQTILKAAKTNGPQGSSGDGKIFVIPLEDC
                     IRIRTGERGPSAI"
     gene            1739..>2580
                     /locus_tag="PLG42_10530"
     CDS             1739..>2580
                     /locus_tag="PLG42_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOU31685.1"
                     /translation="MLVKTFASAVTGIDAVTVTIEVNVSRGIRFFLVGLPDVAVKESQ
                     QRIESALRALGFHWPGKQVVINMAPADIRKEGSAYDLPLAAGILAADEKISNGLLDSF
                     IMMGELSLDGTLQPVRGVLPIAIRARDEGFKGVIVPLKNAMEAAVVNGIDVYGMSSLA
                     DVIDFFNGRKRFEPVKVDLNELFNREANKYDVDFSDVKGQESVKRALEVAAAGNHNVI
                     MVGPPGAGKTMLARRLPTIIPPLMLEEALETTKIHSVAGKIDDNVSLMTRRPFRSPHH
                     TISDV"
BASE COUNT          703 a          507 c          646 g          724 t
ORIGIN      
        1 caactgcatg gatgcttaca tcaaccgcac ttgtactttt aatggtcccc ggccttgcat
       61 tgttctatgg cggactggta agatcgaaga atgtactggg cacaatgatg cacagttttg
      121 ccgctatggg aataataact gtcctttgga tggttgctgg ttattcctta agctttggaa
      181 gaagcgtatt tggtggtttc tttggatgga ataatgacta tttttttctt aaaggaatcg
      241 actcaaatat acttgatgcc ggtatacctg aatatgtttt taccatgttc cagggaaagt
      301 ttgcaattat tacgccggca ctcattgcag gagcttttgc cgaaaggata tcttttaagg
      361 catactgctt cttcatagcg atctggagtc ttctagtata tgatccgtta tgtcactggg
      421 tgtgggcttc cgacggtttc ctttttaacc ttggggccaa gggggccatt gacttcgcag
      481 gaggaactgt agttcacatt tcttcaggag taagcggact ggttgctgca atatatcttg
      541 gttccagaag gggatatcca tatcaggtga ttcgacctaa caacctggtt ataaccatgc
      601 ttggtgcagg cctgctttgg gtaggctggt ttggattcaa tgcaggcagc agtatttcga
      661 gcgggttaag cactgcccag gccctgacag caacacaaat agcagcagcc tcaggtgcac
      721 ttacatggat cgttattgaa ggtattcata aggggaaaac cacagcatta ggctttgcaa
      781 gcggtatact cgcaggtttg gttgctgtta cacctgcagc aggtgtggta cagccttacg
      841 gggcattatt tattggtttg tttgctacac tcttttgcta cacggcaata caaattaaaa
      901 ataaactcgg ttatgatgac agtctcgatg ttttcgggat acacggtgtc ggaggaatta
      961 caggtgccct gctgctcact ttctttatca gaccttcgtg gatggctgat gcggcaaagg
     1021 tatcacacac aggttcatgg acaatctgga accagctcgg agtccagtcc attgctgtcg
     1081 gagtggcaat agcctatgct gccataatga ctttggtaat aatttacgta ctaaacaaag
     1141 taatgaaaat aagatctgac gaagaagatg aaatggccgg actcgacaga tcttaccatg
     1201 gagaaagagg ttatggaatg gttaacccaa gctgattaaa attttaaaat atcataacat
     1261 gaaattaata actgcaataa ttagagaagt ccagctggac aaggtcagag aagcccttat
     1321 agaggcaggg attaacagga tcacagttag tcttgtttca ggaaatggcc gacagagaat
     1381 tgaggaaatg tataggggtc ttacaattat acccgatctt ataccgaaga tcaggattga
     1441 aattgccgtg aatgacgaat cggttgaaaa agcaatacag acaatattga aagcggcaaa
     1501 gacaaatggc ccgcaaggtt cttcaggtga tgggaaaatt tttgtcattc ctcttgaaga
     1561 ttgtataaga ataaggacag gtgagagagg tccttcagca atttgactca taggttggtt
     1621 aggttaggat ggaagatccg gtaagttcaa ttaacggatc ttcctttttt cttttatttt
     1681 ttgtctttta tctttgtttt cctatatttg ttatgcattt cctctaaagt gcttaaacat
     1741 gctggtaaaa acatttgcta gtgcagtaac cggcatagat gccgttactg ttaccattga
     1801 ggttaacgta tcccgcggaa taaggttctt tctggtaggg cttcccgatg tagcagttaa
     1861 ggagagccag cagagaatag aatcggctct cagggctttg gggtttcact ggccaggcaa
     1921 gcaggtggtt ataaatatgg ctccggctga tatccggaaa gagggctcgg catacgacct
     1981 gccacttgct gctggaattc ttgcagctga cgaaaagatc tcaaatggcc ttctcgattc
     2041 gtttatcatg atgggagaac tctcactcga cggcactctg cagcctgtaa ggggagtgct
     2101 ccctatagcc atcagagcca gggatgaagg cttcaaggga gtgattgtcc cgttgaaaaa
     2161 tgcaatggag gctgcagttg ttaacggtat agatgtctac ggaatgagtt cactggctga
     2221 tgtgatagat ttctttaatg gaaggaagag attcgaaccg gtgaaggtgg acctgaatga
     2281 acttttcaac cgtgaagcaa acaagtatga tgttgatttc tccgatgtaa aggggcagga
     2341 gagtgtcaag agggcactgg aagtggctgc tgcaggaaac cataatgtta taatggtcgg
     2401 gcctccggga gcaggtaaaa caatgcttgc ccgccgcctt cccaccataa taccacccct
     2461 gatgctggag gaagctctgg aaaccacaaa aattcactct gtagcgggta aaatagatga
     2521 taatgtctca cttatgaccc gccgcccttt ccgctcaccc caccatacaa tatccgatgt
//
LOCUS       DAOSNB010000320         2547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_40702_length_2547_cov_4.036517, whole genome shotgun sequence.
ACCESSION   DAOSNB010000320 DAOSNB010000000
VERSION     DAOSNB010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40702_length_2547_cov_4.036517"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PLG42_10535"
     CDS             <1..1253
                     /locus_tag="PLG42_10535"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOU31686.1"
                     /translation="TLEKEGCTVSYTDDPQTIPPLYTDILPKEKMLVVFTPAIPPENR
                     ILEFFRWGGYNVYKRSEVLGEISDHTDTIAVAGTHGKTSVSTLIAHILHSSQTGCSAF
                     LGGISCNYGTNLLLSGSRFTVMEADEYDRSFHRLSPLYAVITSADADHLDIYGDYRTM
                     LEAYNEFCRKIRPGGSLILNESIKNVIKPPDNLKVFTYGFSKECSFRINNVKRDDDFY
                     VFDISYFGGELKNLAYRFPGLKNIENAAAASSVALLCGVDGEEIRNSLKSFKGVRRRF
                     DIRINKPGLVYIDDYAHHPEEIKAFVTAVREHYGKRKITAIFQPHLYSRTRDHAEGFA
                     AILDQLDEVILLPVYPAREKPLEGVSSEMIYERMKLKNKKMLEMKDLPAALDVKNLDI
                     LLTIGAGDIDRLVSPIEETLNRMR"
     gene            1257..2033
                     /locus_tag="PLG42_10540"
     CDS             1257..2033
                     /locus_tag="PLG42_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31687.1"
                     /translation="MRILKRILVFLASSYLVVAPVYLTYLIRNERCRGVNIYVNELPD
                     FNLITEREIYGIINSGNPSITGTKLKDLRICDIEDRLKKLQELRVVEVYFTVDGNISI
                     YVLQRNPILRVFAAGGDYFLDDEGVLIRKKRPGTPRLHVAGGNITVSQKMLNGISVLD
                     TAIKKSVLKDIYHIIKYIRNDRFWSAQIDQIWVDGESEINLIPRTGNHIIHLGSAEDF
                     VGKLENLEAFYTKILPLAGWDTYKRINLEFKGQIVCNKYQ"
     gene            2054..>2547
                     /locus_tag="PLG42_10545"
     CDS             2054..>2547
                     /locus_tag="PLG42_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOU31688.1"
                     /translation="MNRKEEYVAAVDIGTTKIVAIVGTRNENGRIEILGLSKAPSRGV
                     KRGVVLNIEETVNAIETTVNDVKKRSGIDFSEVFVGIAGQHIKSMKSRGYILRDNYED
                     EITKDEVFRLIEDMYKIHVDIGEEIIHVIPQNFIVDNETGVKSPIGMCGRRLEANFHI
                     VIGQV"
BASE COUNT          829 a          460 c          567 g          691 t
ORIGIN      
        1 aaacacttga gaaagaaggc tgcacggttt catatactga cgacccacag actattcctc
       61 ctctttatac agatatactg ccgaaagaaa aaatgctggt tgtcttcacc cctgccattc
      121 ctcctgaaaa cagaattctc gaatttttca gatggggcgg atataacgtt tataaaaggt
      181 cggaagtgct cggggaaatc tcagatcaca ccgacacaat agccgttgca ggaacgcatg
      241 ggaaaacctc tgtttcaaca cttattgcgc atatacttca ttcatcacaa actggatgct
      301 ctgcattcct tggcggaata tcctgcaatt acggaaccaa tcttttactg tcgggcagtc
      361 gctttactgt gatggaagcc gatgaatacg acagatcttt ccacaggttg tctccgctgt
      421 atgctgttat aacttccgcc gatgccgacc atcttgatat ttatggagac tacagaacaa
      481 tgcttgaggc atataatgaa ttctgcagga aaatccgacc cggaggatct cttatcctga
      541 atgagtcaat aaaaaatgtt ataaaacctc ctgataattt aaaggttttt acttatggct
      601 tttccaaaga atgctcgttc agaataaata atgttaaaag agatgatgac ttttatgttt
      661 ttgacatttc atattttggt ggtgaattga aaaatctggc ataccgtttt cccggtttga
      721 aaaatattga gaatgctgct gccgcatcat cagtggcact tctatgcgga gtggacggag
      781 aagaaatcag aaattcatta aaatcattca agggtgtaag gagaagattt gatatccgta
      841 taaacaaacc tggactggtc tacatcgacg attatgcaca tcaccctgaa gaaataaaag
      901 cttttgtaac agcagtaagg gaacattacg gtaaaaggaa gatcactgca attttccagc
      961 ctcatctcta ttcccgtacc cgcgaccatg ctgaaggttt tgctgccata ctcgatcagc
     1021 ttgatgaggt cattcttctg cctgtatacc ctgcaaggga aaaacctctg gaaggggtat
     1081 catcagaaat gatttatgag agaatgaaat tgaaaaacaa gaagatgctg gaaatgaaag
     1141 atttacctgc agcacttgat gtgaagaacc tggatatact cctcacaata ggagctggag
     1201 atattgacag acttgtttct ccaattgaag aaacccttaa ccggatgagg taaagtatga
     1261 ggatattaaa aagaatattg gtttttcttg cctccagtta tctggttgtg gcacctgttt
     1321 atctgacata cctcataaga aatgagcggt gtcggggagt aaatatttac gttaacgaac
     1381 ttcctgattt caatcttatt acagaaaggg aaatttatgg aattattaac agcggcaatc
     1441 cttccataac gggaacaaaa ttaaaagacc tgagaatctg tgatatagaa gaccgattga
     1501 agaaactcca agaattgaga gttgttgagg tctattttac ggttgatggc aatatttcca
     1561 tatatgtgct tcagaggaat cctatattga gggtatttgc agcaggtgga gactattttc
     1621 tcgatgatga gggagttttg ataaggaaaa agagacctgg tacacccagg ctccatgttg
     1681 caggtggtaa catcactgta tcacagaaga tgcttaatgg tataagtgta ctggatactg
     1741 caataaaaaa gagtgtgctt aaagacattt accatatcat aaaatatatc aggaacgaca
     1801 ggttctggtc ggcacagatt gaccagatat gggttgatgg tgaaagtgag attaatctca
     1861 ttccgagaac gggaaatcat attatccatc tgggctcagc ggaagatttc gttggaaaac
     1921 tcgaaaatct tgaggctttt tatacgaaga ttcttccttt agccggatgg gatacttata
     1981 aaagaataaa ccttgaattc aaaggacaga tagtatgcaa taaataccag taattaataa
     2041 tcatttctca gccatgaaca gaaaagaaga atatgtagca gcagtcgaca taggcaccac
     2101 aaaaattgtg gcaattgtcg ggaccaggaa tgaaaacgga aggatagaaa tcctgggact
     2161 aagcaaagct ccttcacgag gggttaaaag aggtgtcgta ctcaacattg aagaaacggt
     2221 taatgccatc gagacaaccg taaatgatgt gaaaaaacgt tcggggattg acttcagcga
     2281 ggtttttgta ggtattgccg ggcagcatat caaaagcatg aagagcagag gttacatcct
     2341 tcgcgacaat tatgaggatg agataacaaa ggatgaagtc ttcaggttga tagaggacat
     2401 gtacaaaatc catgttgata tcggggagga gataattcat gtcattcccc agaatttcat
     2461 agtggataat gaaaccggcg tcaaaagccc tatcggtatg tgtggaagaa ggcttgaagc
     2521 aaacttccat atcgtaattg gacaggt
//
LOCUS       DAOSNB010000321         2538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_40885_length_2538_cov_4.097060, whole genome shotgun sequence.
ACCESSION   DAOSNB010000321 DAOSNB010000000
VERSION     DAOSNB010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40885_length_2538_cov_4.097060"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLG42_10550"
     CDS             <1..1107
                     /locus_tag="PLG42_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOU31689.1"
                     /translation="KAKERFYLDKNGNPVSKAKLYQLRDGIFEAIFDRFYKDPTLVAW
                     GEENRDWGGAFAVYRGLTEALPYHRLFNTPIAEGAIVGAAIGYGMCGGRVIAEIMYCD
                     FLGRAGDEVFNQLPKWQAMSGNLIKMPVVVRVSVGSKYGAQHSQDWSSLVAHIPGLKV
                     VFPATPYDAKGLMNSALNGTDPVIFFESQRIYDVGEMFHEGGVPEGYYEIPLGEPDIK
                     RKGNDITILTIGATLYTAVKAADILKEKYGLEAEIIDARTLVPFNYEPVIESVKKTGR
                     IVLASDASSRGSFLKEMAQTITELAFDYLDAPPVVVGSRNWIVPAYEMEKYFFPQPEW
                     IIDAIHCRIIPLKNHNPVHIFSDETVIKLNREGV"
     gene            1168..2418
                     /locus_tag="PLG42_10555"
     CDS             1168..2418
                     /locus_tag="PLG42_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31690.1"
                     /translation="MKIRINNNYLLSHFPPPDELLKIFSGKDLPEYREVNAHIHTPFS
                     FSAFPGMEMIFSMATEENIKVLGINDFYVADGFEEFFKGCISNGIFPLFNIEFIGLMK
                     ECQQKGIRINDPNNPGRIYFSGKGLDYPFHLDTQLLAELSAVKEKTQEQLRQMLDKLN
                     VLITRSATGLSLSFEEVKKRFARELVRERHLAKAVRILAAENFATQEEQLKFIETLYG
                     GKKSKFDMSMPAALENEIRSNLLKAGGEAFVAEDEESFLEPEKIIKIIKSAGGIPCYP
                     VLLDDARGNFTEFEKDYDSLYHSLISLNTGCIELIPGRNDHDILRDFAEFFYRKGFII
                     TFGTEHNTPELEPLTVSARGHKELDPELKKISWKGACVIAAHQYLRAKGKEGYLITGE
                     PDMEIRNRFEELGRCVIEYYLKNY"
BASE COUNT          800 a          533 c          568 g          637 t
ORIGIN      
        1 aaagcgaaag agagatttta tctcgacaaa aatggcaacc ctgtatcaaa agcaaagctg
       61 tatcaacttc gcgacggtat tttcgaagca atatttgaca ggttttacaa ggaccccacc
      121 cttgttgcct ggggtgagga aaaccgtgac tggggaggtg ccttcgcggt ttatcgcgga
      181 cttacagaag cacttccata tcacagattg tttaacactc ccattgcaga aggtgcaata
      241 gttggtgcag ctattggcta cggaatgtgt ggcggcaggg tgatagcaga aataatgtac
      301 tgcgatttcc ttggcagagc aggtgacgag gtgttcaatc aacttcctaa atggcaggca
      361 atgagtggca atctcattaa gatgcctgtt gtggtacgag tatcggttgg ttcaaaatat
      421 ggcgcacagc attcacagga ctggtcgtca cttgtggcac atatacccgg attgaaagtt
      481 gtcttccctg ccacacctta cgatgcaaaa ggtctgatga acagtgccct caacggaact
      541 gatcctgtta tcttcttcga aagccagaga atatacgatg tgggcgaaat gttccacgaa
      601 ggcggcgtac ccgaaggtta ctacgagatc ccactcggag aaccagatat caaaagaaaa
      661 ggaaacgaca taacaatact caccataggt gcaacactct ataccgcagt aaaagctgct
      721 gacatattga aagaaaaata cggactcgaa gcagaaataa ttgatgcacg tacactggtg
      781 cccttcaact acgaaccagt aattgaatcg gtaaaaaaaa cgggacggat cgttcttgca
      841 agcgatgcgt catcaagggg ctctttcctc aaggaaatgg cacagacaat aacagagctt
      901 gcattcgatt accttgatgc ccctcccgta gtggttggct cacgcaactg gattgtgccg
      961 gcatacgaga tggagaaata tttcttccca cagcccgaat ggattattga tgccattcac
     1021 tgcagaatca tacccctgaa aaaccataac cctgtacaca tcttctccga tgaaaccgta
     1081 ataaaactta accgtgaagg tgtttagcaa agattcatat ctttgactgc tgttatggac
     1141 tattcagata aataaaaacc cggaggcatg aaaattagga taaacaacaa ttatttactg
     1201 tcacacttcc cgccgccgga tgaactcctg aaaatattct caggtaaaga tcttcctgaa
     1261 taccgcgaag taaacgctca tatccatact ccattctctt tcagtgcctt ccccggtatg
     1321 gagatgattt tctccatggc gaccgaagaa aatatcaagg ttctgggaat aaacgatttt
     1381 tacgttgccg acggatttga agaattcttc aaaggctgca tcagcaacgg aatcttccct
     1441 ctttttaaca tagagtttat cggcctgatg aaagagtgcc agcaaaaggg aataagaatc
     1501 aacgacccga acaatccggg acgaatctat ttctcaggca aaggactcga ttatccgttt
     1561 cacctcgata ctcaactgct ggctgaactt tcagcagtga aggaaaaaac ccaggaacag
     1621 ctccgacaga tgctcgacaa actcaatgtt ctgattacca gatcagcgac cggtcttagc
     1681 ctcagctttg aagaggttaa aaagagattt gcgagggaac tggtaaggga aaggcatctg
     1741 gcaaaagctg taagaatact tgcagcagaa aactttgcca ctcaggagga gcagttaaag
     1801 tttattgaaa ccctctacgg tggcaaaaaa tcaaaatttg atatgagcat gcctgcagct
     1861 ctcgagaatg aaatccgctc aaatctgcta aaggctggag gtgaagcttt tgttgcagaa
     1921 gatgaagaat ctttccttga acctgagaaa attataaaaa ttataaaaag tgctggtgga
     1981 attccctgtt accctgtatt gcttgatgat gctcggggaa acttcaccga atttgagaaa
     2041 gattatgaca gtctgtacca ttcattgata agtctcaaca caggatgtat tgaacttatc
     2101 cccggaagaa acgaccatga tattctcaga gattttgcag agttctttta caggaaaggc
     2161 tttataataa ccttcggtac cgagcacaac actcctgagc ttgaaccatt gactgttagc
     2221 gcacggggtc acaaagagct cgatcctgaa ttgaagaaaa tttcatggaa aggtgcatgc
     2281 gtaattgctg ctcaccagta tctcagggct aagggaaaag aaggttattt aataacaggt
     2341 gagcctgata tggaaattcg taatagattt gaagaacttg gccggtgcgt aatagagtat
     2401 tatttaaaaa attactagaa tgaaacctga aattgccgaa ctactggaaa tatcccgctt
     2461 ttatggcagc aacaaggatt atgttatagc gggtggcggt aacacttctt ttaagtacga
     2521 aaaaactcta tggattaa
//
LOCUS       DAOSNB010000322         2537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_40903_length_2537_cov_4.450846, whole genome shotgun sequence.
ACCESSION   DAOSNB010000322 DAOSNB010000000
VERSION     DAOSNB010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40903_length_2537_cov_4.450846"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..539)
                     /locus_tag="PLG42_10560"
     CDS             complement(87..539)
                     /locus_tag="PLG42_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOU31691.1"
                     /translation="MSAGIIRVQCDNVIENNTMSSPEEVRIDKFLWAVRVFKSRSAAA
                     DACKKGRIFVNSIPAKPSRTVSIKDIITVKKLPVIYTYQVISLTVNRVPAKNVNLYVT
                     DLTPEDEKVKLYSVKQTLHGKRPKGTGRPTKKERREIDRLFNSDTASW"
     gene            complement(508..1125)
                     /gene="pth"
                     /locus_tag="PLG42_10565"
     CDS             complement(508..1125)
                     /gene="pth"
                     /locus_tag="PLG42_10565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOU31692.1"
                     /translation="MKYLIAGLGNIGEEYKDTRHNVGFLVLDAIAGEFNINFSDKRYG
                     FIAYLNYRGRQLVLLKPSTYMNLSGNAVNYWLKKEKIDLENLLVIVDDVALPLGTIRM
                     RPKGSDGGHNGLAHITSVLGTNEYARIRFGIGNEFAKGRQKEYVLSPWTPEEKEILKE
                     KIPYVTEMIKSFVTAGLEITMTKYNTPRSRQKPSEDVSRDNKSSV"
     gene            complement(1156..1830)
                     /locus_tag="PLG42_10570"
     CDS             complement(1156..1830)
                     /locus_tag="PLG42_10570"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOU31693.1"
                     /translation="MKTLQITGTLRKELGKKDAVRLRKSNNVPCVLYGGKENIHFYTH
                     ENSFLKLIYTEDVHLVNINLNGKQYSTLLKDIQFHPVTDRVIHADFIEVSEDKPVTVS
                     LPVRVTGDSVGVKAGGKLRINKRYLTVKSLIKNIPDHITVDITDLKIHDSIKVGDISI
                     PDVEILEPKNVAVVNIVTSRVAQKAEEAETAEAAAAAGTAEKAGEKPAEKGGEKAAEK
                     TAEEKK"
     gene            complement(1931..>2537)
                     /gene="prs"
                     /locus_tag="PLG42_10575"
     CDS             complement(1931..>2537)
                     /gene="prs"
                     /locus_tag="PLG42_10575"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOU31694.1"
                     /translation="KLSADLLSAAGVDRIITMDLHADQIQGFFNVPVDHLFGSAIFAP
                     YIESLKLDHLAVAAPDMGGSKRANAYARHLQADLVLCYKLRKKPNVVEEMSIIGEVEG
                     KNIVMIDDMVDTGSSMVLAAQMMKERGAKSIRAFATHPVLSGNAVERINNSPIEELVV
                     TDSLPVKINSPKIKVLSIAKILANTIFAVTTNQSISTNFVF"
BASE COUNT          609 a          520 c          525 g          883 t
ORIGIN      
        1 atctggagcg gcaattttat gagggttctc cgcaaagcag aggaatatgc cggcaggcgg
       61 taactcttta tctggttaag gaatttttac caggaagctg tgtctgaatt aaaaagtcta
      121 tctatttccc ttctttcttt tttcgtgggt cggcctgtgc cttttggcct ttttccgtgg
      181 agagtctgtt tgactgaata tagcttaacc ttttcatctt caggggttag gtcggttaca
      241 taaagattta cgttctttgc tggaacccgg ttaacagtga gacttattac ctggtatgta
      301 tatattacag gaagtttttt cactgttatg atgtctttta tggaaacagt tctggatggt
      361 ttagcaggga tgctatttac gaagattctc ccttttttac aggcatctgc tgcagcggaa
      421 cgtgatttga atactcttac tgcccagagg aacttgtcga ttcttacctc ttcaggtgag
      481 ctcatggtat tattttcaat aacattatca cactgaactc ttattatccc ggctgacatc
      541 ttctgacggc ttttgccggg agcggggtgt gttgtatttt gtcattgtta tttcgaggcc
      601 ggcagtgacg aatgatttta tcatttccgt tacatatgga attttctcct ttagaatctc
      661 tttctcttca ggtgtccatg ggctcagtac atactctttc tgccttcctt ttgcgaattc
      721 atttccaata ccgaatctta ttcttgcata ttcgtttgtt cccagcactg aagttatatg
      781 tgcaagtccg ttatgtccgc cgtcacttcc tttcggacgc attctgatgg tccccagtgg
      841 cagtgctacg tcgtcgacta ttacaaggag gttttctaaa tcgatttttt cttttttcag
      901 ccagtagttt actgcattgc cgctgagatt catgtaagtt gaaggtttaa gcaggactaa
      961 ttgtcttccc ctgtaattca ggtaagcaat aaagccatat cttttgtcgg agaaattaat
     1021 gttgaattca cctgcaatag catcaagtac caggaatccg acattatggc gggtatcttt
     1081 atactcttcg ccaatattcc cgagtccagc tatcagatat ttcaagttat gtagagaatt
     1141 gtctgaggag tggtattatt ttttctcttc agcagttttt tcagcggctt tttcaccacc
     1201 tttttcagca ggtttctcac cggctttttc agctgttcct gcagcagcag ctgcttcagc
     1261 tgtttcagcc tcttcggctt tctgggctac tcttgatgta actatattaa cgaccgcaac
     1321 gtttttcggt tccagaatct caacatcagg tatagagata tctccaacct ttatagaatc
     1381 gtgtatcttc aggtcggtga tatcaactgt aatatggtca ggaatgtttt ttatcagtga
     1441 cttaacagtt agataccttt tgttgattct cagtttaccg ccagccttaa cacccaccga
     1501 gtcgcctgta acccttacag gcagcgatac tgttactggt ttatcttctg aaacttcaat
     1561 gaaatcggcg tgaatgaccc tgtcggtaac tggatggaac tggatatctt tcagcagagt
     1621 actatactgc tttccgttga ggttaatatt tacaagatga acatcttcgg tataaatcag
     1681 tttgaggaag ctgttttcgt gtgtgtaaaa atgaatattt tcttttcctc cgtataatac
     1741 acaggggaca ttatttgact ttctgagtct gacagcatcc tttttcccca gttctttcct
     1801 taaagtacct gttatctgta aagttttcat tttattaata attgttgtgc tactcaattc
     1861 cgcggggcgg aatcccatta cacgttaata aatttctttc aaaattggct gcaaatatat
     1921 tacaaatcgg ttaaaataca aaattggtac ttattgactg gttggttgtg actgcaaaga
     1981 ttgtattggc aagaattttt gctatggaga gcacttttat ttttggagaa tttattttta
     2041 cgggcaatga atctgttact acaagttctt caataggaga gttatttatt ctttccactg
     2101 catttcctga tagaacagga tgtgttgcaa aagccctgat actttttgcg ccccgttctt
     2161 tcatcatctg tgcggcaaga accattgaac ttccggtatc gaccatatcg tcgatcatta
     2221 cgatgttttt gccctcaacc tcaccgatta tagacatttc ttctaccacg ttaggttttt
     2281 ttctgagttt gtaacacagt acaaggtcgg cctgaaggtg gcgggcatat gcattagctc
     2341 ttttggaacc acccatatca ggtgctgcta cggcaagatg gtcgagtttc agactttcta
     2401 tatatggagc aaagatggcc gagccgaaga ggtggtcgac agggacatta aagaaaccct
     2461 gtatctgatc ggcatgcaga tccattgtta taatgcggtc gacaccggca gcactaagga
     2521 ggtcggcaga aagtttc
//
LOCUS       DAOSNB010000323         2530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_41054_length_2530_cov_4.841212, whole genome shotgun sequence.
ACCESSION   DAOSNB010000323 DAOSNB010000000
VERSION     DAOSNB010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41054_length_2530_cov_4.841212"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLG42_10580"
     CDS             <1..1145
                     /locus_tag="PLG42_10580"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOU31695.1"
                     /translation="KGPHYRALIITGQNSTDWVASTPVLKQILEKTGIFECSILISPT
                     PGGEWDKFRPAWDNYDLVVLNYNGEKWPETIRNGFISYVNNGGGVLVYHSASIAFPDW
                     KEYNEICGISGWDNRSEKDGPYIYFRGNRMVLDSSAGPAGWHGEPREFEVRIRNTEHP
                     VTRGLPVRWMHAKDLLYARLRGPAKNMEVLATAYCDTTGGGTGRDEPVMMAITYGKGR
                     VFHTTLGFPGDGTSPALQCAGFITTLQRGAEWAASGKVTQPVPRDFPTVSGVVLRTEM
                     KPITLKEEIENLAKYEIGMSTRYYTGLQDRIRKAAGDPDKLLEIEKMMVKLLENDKAT
                     IESKKLVLRELSWMGTDYCIPTIKKLQENAELKDEVDFALERLGVK"
     gene            1155..2084
                     /locus_tag="PLG42_10585"
     CDS             1155..2084
                     /locus_tag="PLG42_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOU31696.1"
                     /translation="MLSVIIPVYNEEAVLDELIRRTVPAIESFTTDYEIIFTDDGSTD
                     GSLKKLLSFREKNPKIKILSLSRNFGHQAAYTAGLEHAAGDLIAMMDADLQDPPELLI
                     EMYRKITVEGFDAVYGRKIGRKGKKRRNFFMYLFHLIFKNIAEISNIENAGNFSMMTR
                     KVVEALLSMREKIRYLPGMRSFVGFRQGYVDYVRDDRYTGKPKMSTGKLFRLAGDAIF
                     SFSRLPIKLCFILGLTGVIIFLAAGIYVIIAKILNFAIPGWSSILLSIYFLGSIQLVF
                     LGIIGEYVYRNYKESQNRPIYFIKEFYGSEPKN"
     gene            2084..>2530
                     /locus_tag="PLG42_10590"
     CDS             2084..>2530
                     /locus_tag="PLG42_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31697.1"
                     /translation="MNRAWHKYAFIVVALLLLAMMLYRSREAGVSCDEVLHYNQSVKV
                     YNYFRSGGTDRTALQDSEQYLKYYGQSYDNITTFLIKSFGIEDIYSFRHLMSSLAGWL
                     AIIVTAIFAVWLSGYSAGLLVLLLFAVSPTFLGHSQNNLKDIPFALG"
BASE COUNT          758 a          476 c          598 g          698 t
ORIGIN      
        1 aaaaaggccc gcattacagg gcactaataa tcacgggaca gaattcgacc gactgggttg
       61 caagcactcc tgtattgaag cagattctcg aaaaaactgg aatttttgaa tgcagcatat
      121 taatttctcc taccccggga ggtgaatggg acaaattccg gcctgcatgg gataattatg
      181 atctggtagt tttaaattat aacggcgaga aatggcctga aacaatacga aatggtttta
      241 tcagttacgt aaataacggt ggaggcgttc tggtatatca ttctgcctct attgcttttc
      301 ccgactggaa ggaatataat gagatatgcg gtatatcggg ctgggataac aggtcggaaa
      361 aggatggacc ctatatatat ttcagaggta atcggatggt gcttgactcc tcagcaggac
      421 cagcagggtg gcatggcgaa ccgcgtgagt ttgaagtaag gataagaaac accgaacatc
      481 ctgttaccag aggtttaccg gtaagatgga tgcatgcaaa agatttgctc tatgcccggt
      541 tgaggggtcc cgctaaaaat atggaagtcc ttgccacagc ttattgtgac accaccgggg
      601 gaggcacagg cagggatgag cctgttatga tggcaataac ctacggtaaa ggcagggttt
      661 tccatactac tcttggattc cccggtgatg gaaccagtcc cgcacttcag tgtgccggct
      721 tcatcacaac tctgcaacgt ggagccgaat gggcagcctc gggaaaagtg acacaaccgg
      781 ttccacgtga ttttcctaca gtttcaggtg ttgttttacg tacagagatg aaaccgatta
      841 cacttaagga agaaattgaa aatcttgcaa aatatgagat cggaatgagt acccggtatt
      901 atacaggtct gcaggacaga atcagaaaag ccgcaggtga ccctgacaaa ttactggaga
      961 tagaaaaaat gatggtcaaa cttctggaaa atgacaaggc aacaatagaa tcaaagaaac
     1021 ttgtattacg tgaactcagc tggatgggaa ccgattattg cattcccaca ataaaaaagc
     1081 tgcaggaaaa tgccgagttg aaagatgagg tagattttgc tcttgaacga ctaggtgtaa
     1141 aataatcaac tggaatgtta tcggttatca ttcctgttta taacgaagaa gcagttcttg
     1201 atgagctgat acgcagaacg gtacctgcaa ttgaatcttt tacaaccgat tatgagataa
     1261 ttttcaccga cgacggcagt actgacggca gccttaagaa attactttcg ttccgtgaga
     1321 aaaaccctaa aataaaaata ttgtcccttt caagaaattt tggccatcag gcagcttaca
     1381 cagctgggct ggagcatgct gcgggagatt taattgccat gatggatgcc gaccttcagg
     1441 acccgcctga actgcttata gaaatgtaca gaaagataac cgttgaagga ttcgatgctg
     1501 tatatggaag aaaaatagga aggaagggga aaaagcgaag gaattttttt atgtatttat
     1561 ttcacctgat ctttaaaaac atagctgaaa taagcaatat agaaaatgcc ggtaatttct
     1621 ccatgatgac ccgcaaggtg gtagaggcac tactttcaat gagagagaag ataagatacc
     1681 ttccgggtat gcggtctttt gtgggcttca ggcaaggata tgttgattat gtaagggatg
     1741 acagatacac cgggaaacca aaaatgagta ccggtaaact ctttcggctt gccggggatg
     1801 caatattttc attttcaagg ttgccaataa aattatgttt catactgggg ctgacagggg
     1861 taattatttt tctggctgcc ggaatatacg taattatcgc aaagatttta aattttgcaa
     1921 taccgggttg gtcatcaata cttctgagta tttattttct gggctccatt caacttgttt
     1981 ttctaggtat cattggcgaa tatgtttacc gtaattacaa agagtcgcag aacagaccca
     2041 tatatttcat aaaggagttt tacggaagtg aacccaaaaa ttaatgaaca gagcatggca
     2101 caaatatgca ttcattgttg tcgctctgct tttgcttgcc atgatgcttt acagaagcag
     2161 ggaagcaggc gttagctgtg atgaagtgct tcattataac cagtctgtca aggtatacaa
     2221 ttatttcagg tcaggcggaa ccgatcgcac agctttacag gattcagaac agtatctcaa
     2281 atattatgga cagtcgtatg ataacattac gacttttctt atcaagtctt tcggtataga
     2341 ggatatctat tctttccgtc atctgatgtc gtctctggca ggatggctgg caataattgt
     2401 aacggcaatt tttgcggtat ggctatcggg ttactctgcc gggcttttgg tgctgcttct
     2461 gtttgccgtt tctcctacat ttcttggtca ttcacagaat aaccttaaag atataccatt
     2521 tgcacttggc
//
LOCUS       DAOSNB010000324         2435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_43211_length_2435_cov_3.998319, whole genome shotgun sequence.
ACCESSION   DAOSNB010000324 DAOSNB010000000
VERSION     DAOSNB010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43211_length_2435_cov_3.998319"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLG42_10595"
     CDS             <1..747
                     /locus_tag="PLG42_10595"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOU31698.1"
                     /translation="DDLYRQYSVQLQKVQKAQSEFDRNSFLYSQSLIPQAEYENSRYQ
                     LSVESENLRRIVSSAVTGWQYDLNQRRNDSVRMVAEYCHYNEELSNRVVTTPVAGEIV
                     ISSDIQEGSTIAANQKILEISPEGELLANVLVKPADIGFIREGQLVRIQVDAFRYNEW
                     GMIEGEIIDVSDDLFIEGNTGAFFRVRCRLKNHTLSLKNGYTAIMKKGMSINARIIVT
                     RRSLFNLLFDRIHKWLNPYMNNPENKSNEV"
     gene            737..>2435
                     /locus_tag="PLG42_10600"
     CDS             737..>2435
                     /locus_tag="PLG42_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOU31699.1"
                     /translation="MRYKIKQRDITDCGAACLASVAAYYRFDIPVARIRQLAGTDRKG
                     TNVLGLLEAAGRMGFTAKGVKGDMDCLGKIPKPAIAHFIVRKVLHHYVVLTKVSQKWV
                     EIMDPSDGEIHRLTYEEFREQWTGVLVIMVPGEKFRPGNEKISFQKRFFELLKPETGV
                     FIQTLIGSLAFSVLGIGMSLYLGKLVDHVLPGGNLNLLNLLGVAMVLVILLRVLLSFF
                     QSVFILKTGQRIDAKLILGYYQHLLKLPQSFFDNMRTGEIISRIGDAVKIRLFINEVA
                     VGLLLNILILVVSFIIMFTFFWKLALIMFIIIPLYTGIYIVSNTLNKKVQRKVMEKAA
                     DLEAHLVESLNAAGTVKRLTLEDFSNIRTETRFVDFLNTIYRSGLNSIFSSGTSGTVS
                     QVFSLVILWAGAAFAIHNEITAGELLAFYSVTGYFTGPVAGIIGFNRTLQDAKIAADR
                     LFEIFDLEPEESSRGIELTREMIGDIEFENVKFRYGTRRNVFESLSLTIRRNEITAVV
                     GESGSGKTTLAVLLQNLYPLQSGNIRIGRNDIRHFTNDSVRKLISVVPQKIDLFAGSV
                     "
BASE COUNT          712 a          425 c          586 g          712 t
ORIGIN      
        1 gatgacctgt acaggcagta ttctgttcag cttcagaagg tgcagaaggc ccaaagtgaa
       61 tttgaccgca acagcttcct ttattcccag tcattgatcc cccaggcaga atatgaaaac
      121 agccgttatc agctcagtgt tgaaagtgaa aatctccgac gaattgtctc atctgctgtt
      181 acgggctggc aatatgatct taatcagagg agaaatgatt ctgtaagaat ggtggcagaa
      241 tactgccatt ataacgaaga acttagtaat agagtggtca ccacaccagt tgcaggagaa
      301 atagttatca gttccgacat tcaggaaggg agtactatag ctgcaaatca gaaaattctt
      361 gaaatatcac ctgaggggga gttgctggca aatgttctgg taaagcctgc cgatataggt
      421 tttataaggg aagggcaact tgtaaggatc caggttgatg cttttcgtta taatgaatgg
      481 ggtatgatag aaggggaaat aattgatgta tcggacgatc tttttataga aggaaatacc
      541 ggagcgtttt tcagggtaag gtgcagatta aagaatcata ctctttctct gaaaaacgga
      601 tatacggcaa ttatgaagaa aggcatgagt atcaatgcca ggataatagt tacccgcagg
      661 agtcttttca acctgttgtt cgacagaatt cataaatggc ttaatcctta catgaataac
      721 ccggaaaaca aatctaatga ggtataaaat aaaacagagg gatataacag actgcggtgc
      781 tgcctgtctt gcatcagttg cagcatatta ccggttcgat atacctgttg cacgtatcag
      841 acagcttgca ggtactgacc ggaaaggcac aaatgttctt ggattgttag aggctgccgg
      901 caggatggga ttcacagcaa agggggtaaa gggtgatatg gattgtctcg gtaaaatacc
      961 caaacctgcc attgcccatt ttattgtcag aaaagttttg catcattatg ttgttttgac
     1021 gaaggtttcg cagaaatggg tagaaataat ggacccttcc gatggtgaaa ttcatcgtct
     1081 aacgtacgaa gagttcaggg agcaatggac aggggtgctt gtaataatgg taccgggaga
     1141 gaaattcaga cccggcaatg aaaagataag ttttcagaaa cgatttttcg aattgctgaa
     1201 gcccgaaaca ggggtcttca ttcaaactct tatcggttca cttgcttttt ctgtccttgg
     1261 tataggcatg tcgttatatc tcggaaaact tgtggaccat gttttaccgg gcggaaacct
     1321 caatttgctc aacctcctgg gagtagccat ggttctggtg attttgctga gggtactgct
     1381 gagtttcttt cagtcggttt ttattcttaa aaccgggcag cggattgatg ctaaattgat
     1441 tctcggatat tatcagcatc tgttgaaact tccgcagtca ttttttgata atatgagaac
     1501 aggagagata atatcaagaa taggggatgc agtcaagata cgacttttta ttaacgaggt
     1561 tgctgttggt ctattgctga atattctgat tctggtcgtt tcttttatca taatgtttac
     1621 atttttctgg aaacttgctc ttataatgtt tattatcatt ccattataca ctggtattta
     1681 tattgtttcg aatacgctca ataaaaaggt tcagaggaaa gtaatggaga aggcagcgga
     1741 tcttgaggca catcttgtag agtcactcaa tgctgcagga acagtaaaga ggctcactct
     1801 tgaggacttt tcaaacatca gaacggaaac acgttttgtt gattttctta atacgatata
     1861 caggtcgggt ctgaacagta tcttttcttc aggtacttca ggcacagtgt cgcaggtttt
     1921 ttcgcttgta atattatggg ccggagccgc ttttgccata cataatgaaa tcactgcagg
     1981 ggagcttctc gcattttatt cagttacagg ttatttcacg ggtccagtgg caggaataat
     2041 agggtttaac cggacattac aggatgcaaa gatagctgcc gacaggcttt ttgaaatttt
     2101 tgacctcgag cctgaagaga gcagcagagg cattgaactt accagggaga tgatagggga
     2161 cattgaattt gaaaatgtaa aattcagata tggaacccgc agaaatgttt ttgaatcgtt
     2221 gagcttgacc atcaggagaa atgaaataac ggctgttgta ggtgaaagtg gttcgggtaa
     2281 aaccactctg gcagttctgc ttcagaattt atatcctctt cagtcgggca atatcaggat
     2341 aggccggaat gatatacgcc attttacaaa tgattctgta aggaagttaa tttcggtagt
     2401 tccccagaag atagacctgt ttgcaggtag tgtgc
//
LOCUS       DAOSNB010000325         2403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_44018_length_2403_cov_4.211244, whole genome shotgun sequence.
ACCESSION   DAOSNB010000325 DAOSNB010000000
VERSION     DAOSNB010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44018_length_2403_cov_4.211244"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLG42_10605"
     CDS             <1..1597
                     /locus_tag="PLG42_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31700.1"
                     /translation="KESMTGIINIKYKVIQPVFLVILLLLAGCEAMTNENFQKINSLP
                     VIEPDYINVTIPPNIAPLNFIVRENGRRFFVVAESEKGDYKIKIRSGNGKICFPMKKW
                     RKLTRNSMGGRITVRIFLSGEQDKITKEYQPFSFYVAPEPVDSYLAYRLIYPGYYCWS
                     NLRIVQRCVENFSEKSIIENQVLDMNCINCHSFCNNRPDRFMVHVRGSKGGTYVVSDG
                     KIERKDLKIDPMPGSATYPSWHPSGKYIAFSSNQVRQTFYANPSKSIEVFDLVSDLII
                     YNMEKNSITMVRDDDTTKYLETFPCWSDDGRYLYFCRAPQNVEEPWRDPEKMELIKYS
                     LVRVPFNPETGVYGKTEMVYDAAAEGKSVSFPRVSPDGNFIVFTLAAHGTFPIWHREA
                     DLYILNLQTGELKKMDINSEETESWHEWSSNGRWLVFSSKRLDGRSSRPFYVYFDSRG
                     NTGKPFVLPQKDPERYITMLESFNLPQPVNGKIKVDPRDFVVSSKSQSVRAIAENLAD
                     SLPEWKKERAEMKRNPGERPIHE"
     gene            1614..>2403
                     /locus_tag="PLG42_10610"
     CDS             1614..>2403
                     /locus_tag="PLG42_10610"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HOU31701.1"
                     /translation="MQKSRSISGKFNEFDVWLFIAVFFISSFVFFISAGSYILFFQEQ
                     QHLFVYLPDFLSKFFVKPGGPLELAGEFFTQFYFSTLTGSIILSLILSLPAVVLHFVN
                     RRLKTGNLYSIVLLLIPSLALFLMQSHYYHTMTYNLGYLLVLLYFLFASSSESRKVQV
                     ATLIIYPLFYYITGAYAFIFAGLYIIYTLFYSKGIRKLYYTLLIAALTGVLLIVSESK
                     IFLIPLKQLLTYPLPSVNNTTHRYLTVFLNGFVILYPAVSYVPGS"
BASE COUNT          724 a          495 c          472 g          712 t
ORIGIN      
        1 taaagagagc atgacaggca ttattaatat taaatacaaa gttattcagc cggtttttct
       61 ggttatatta ttgctccttg ccggatgtga agcaatgaca aatgagaact ttcagaaaat
      121 aaacagcctc cctgttattg aacctgacta tataaatgtc acaatacctc ccaacattgc
      181 acctttgaat ttcattgtca gggagaacgg caggcggttt tttgtagttg ctgaatcgga
      241 aaaaggagat tataaaatta aaatccgttc aggtaacggg aaaatctgtt tcccgatgaa
      301 aaaatggaga aagctgacca gaaacagcat gggcggaaga ataacagtaa gaatcttctt
      361 atccggagaa caagacaaaa tcacaaagga atatcaacct ttttcttttt atgtggcccc
      421 tgaacctgtt gactcttatc ttgcgtaccg tttaatctat ccgggatatt attgctggtc
      481 aaaccttaga atagtccaga gatgtgtcga aaacttcagt gaaaaatcaa taatcgagaa
      541 ccaggttctc gacatgaact gcattaactg ccactctttc tgcaacaacc gccccgacag
      601 gtttatggta catgtaagag gctcaaaagg aggaacctat gttgttagtg acggaaaaat
      661 cgagaggaaa gatcttaaaa ttgaccccat gcctggtagt gccacatatc cttcatggca
      721 tccatcagga aaatatattg ctttctcttc caatcaggta aggcagacct tttatgccaa
      781 cccttcaaaa agtattgaag tcttcgacct cgtctctgat ttaatcattt acaacatgga
      841 gaagaacagt attacgatgg tgcgtgatga tgacaccaca aagtatctcg agacttttcc
      901 atgctggtcg gatgacgggc gatacctgta tttctgcagg gcaccccaga atgtggaaga
      961 accatggcgt gaccctgaaa aaatggagct cataaaatac agccttgtaa gagtgccctt
     1021 taaccccgaa actggtgttt acggaaaaac tgaaatggtg tatgatgcag cagcagaagg
     1081 caaaagcgta tcattcccga gagtatcccc cgatggaaat tttattgttt ttacacttgc
     1141 cgctcacgga actttcccca tatggcatcg tgaagccgac ctttacatat taaacctgca
     1201 gacaggagaa ttaaaaaaaa tggatatcaa cagtgaggaa acggagagct ggcatgaatg
     1261 gtcatcgaat ggcaggtggc ttgtattcag cagtaaaagg ctcgacggaa gaagttccag
     1321 accattctat gtctatttcg acagccgggg caatacggga aaaccttttg ttctaccgca
     1381 gaaggacccc gaaaggtata ttaccatgct cgaatcgttc aaccttcctc agcctgtaaa
     1441 cggaaagata aaagtggacc cacgcgactt cgtggtttcc tcaaaaagcc agtctgtaag
     1501 ggccattgct gaaaacctgg ctgattcgct gcctgaatgg aaaaaagaaa gagcagaaat
     1561 gaaaagaaat ccgggtgaaa gacctatcca tgaatgaaaa aattttcaga aaaatgcaga
     1621 aaagcagaag catatcaggc aaatttaacg aattcgatgt gtggcttttt attgcagtct
     1681 ttttcatctc ttcttttgtt ttctttatca gtgccggcag ttatatactt ttttttcagg
     1741 aacaacaaca tcttttcgtc tatctccctg atttcttaag caagttcttt gtaaaacccg
     1801 gagggccgct tgaattagcg ggagaatttt ttacacaatt ctatttcagt acactaacag
     1861 gttctattat tctgtcatta atactgagcc ttcctgctgt tgtactccat ttcgttaacc
     1921 gcaggcttaa aaccggaaac ttatattcta ttgttttact gctgatccca tcattagcgc
     1981 tctttctaat gcagtcgcat tattatcata caatgacata taacctggga tatcttctgg
     2041 ttctcctcta ctttcttttt gcttcatcgt ccgaaagccg gaaagtacag gttgcaacat
     2101 taatcatata cccgttattt tattatataa ctggagctta tgcatttatt tttgctggat
     2161 tgtatattat ttataccctg ttctattcga aaggcattcg taaattatat tatactttgc
     2221 tgattgcagc tctgaccgga gttttgctaa ttgtttctga aagtaagata tttcttatcc
     2281 ctcttaaaca gcttctgact tatcccctgc catctgtgaa caatacaaca cacagatatt
     2341 taacagtttt tcttaacggg tttgtaatat tgtatcccgc cgtcagttat gttcccggtt
     2401 ctt
//
LOCUS       DAOSNB010000326         2391 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_44318_length_2391_cov_5.095034, whole genome shotgun sequence.
ACCESSION   DAOSNB010000326 DAOSNB010000000
VERSION     DAOSNB010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44318_length_2391_cov_5.095034"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PLG42_10615"
     CDS             <1..1784
                     /locus_tag="PLG42_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOU31702.1"
                     /translation="YEGKEYPVYCRKKESLNAPEEIILDVNRIAEGKSYCSVSGLAVS
                     PDNGILAYGADFLSRRKYTIYFVDLKTGTLLPDIIENTSGNIVWANDSKHLFYTVRNP
                     LTLRAEKVMRHRLATLVSDDREVYFEPDETFSVYINRTKSRRYILINSTQTLSTEVRY
                     INSSKPESQPVVFEPRQRNHEYSVDHIGNEFYIRTNADSAYNFKLMKTSEKNTGKENW
                     IDVVPHRKDVLFEEFELFNNFIVLNERIKGLSTLRILNKTDNSDHYIDFGEETYTCSI
                     NINPDPASSVLRYSYSSLTTPNSVYDYDMITREKKLMKQDEVLGGFDPANYESKRLWA
                     RAADSTMIPISLVYRKGFTRNGKAPLLLYGYGAYGNSTEPSFRPNIISLLDRGFVFAI
                     AHVRGGSEMGRYWYEDGKLLKKKNTFTDFNDCAQYLINEKYTSTDRLFAQGGSAGGLL
                     IGAVINMRPDLYKGVIAAVPFVDIVTTMLDPTIPLTTFEWDEWGDPRKKEYYDYMLSY
                     SPYDQVKKQKYPNIIVTTGFWDSQVQYWEPVKWVAKLREMNTGNSLIVMDINMTAGHS
                     GASGRFERNRVTALQYAFILDLAGIRE"
     gene            1800..>2391
                     /locus_tag="PLG42_10620"
     CDS             1800..>2391
                     /locus_tag="PLG42_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HOU31703.1"
                     /translation="MMGRFLLFWTLIFFASCANQSHNLLVEAESFEDTGGWVVDPQFT
                     EQMGSPYLLAHGLGNPVKNASTRISLPSPGRYYIWVRTRDWAPGKWDAPGRFKLSVNG
                     KELKRTLGTGKVTWSWQYAGKVKIKTPEARIELRDLTGFDGRCDAVYLSTIMEPPPEE
                     LEELNFWRMRIKGEDPSKAEKLYYDLVVVGGGIAGCA"
BASE COUNT          765 a          448 c          551 g          627 t
ORIGIN      
        1 attacgaagg aaaggaatac cctgtatact gccggaagaa agagtcatta aatgctccgg
       61 aggaaataat ccttgatgtt aacaggatag ctgaaggtaa aagttattgt tcagtctccg
      121 ggttagcagt aagtccggat aacggaatat tggcttatgg agccgatttt ctaagcaggc
      181 gcaaatacac catctatttt gtcgatctga aaaccggaac ccttctgccc gatattatcg
      241 aaaatacttc aggaaatata gtgtgggcaa acgacagcaa acatctcttc tacacagtaa
      301 gaaatcctct tacactcagg gctgaaaaag tgatgaggca ccggcttgct actcttgttt
      361 cggacgaccg ggaagtctat tttgagcccg atgagacttt cagcgtttat atcaaccgca
      421 caaagagcag aaggtatatc ctgatcaatt caactcaaac tctttcaacc gaagtaagat
      481 atatcaactc ttctaaacct gaaagccagc ccgttgtatt tgaaccgaga caaagaaatc
      541 atgaatacag tgtcgaccat ataggaaatg aattttacat aagaacaaat gccgatagcg
      601 catacaattt caaattaatg aaaacatccg aaaagaatac cggaaaagaa aactggattg
      661 acgttgtacc acatcgaaag gatgttttat tcgaagaatt tgaattattt aacaacttta
      721 ttgtacttaa tgaaagaatc aaaggactga gtactttaag gatcctgaac aaaactgata
      781 acagcgacca ttatatagat ttcggtgaag aaacatatac ctgcagcata aatatcaatc
      841 ccgatccagc ttcgtctgtt ctcaggtaca gctactcatc tcttactact cccaattctg
      901 tgtatgatta cgacatgatt acccgtgaaa agaaactgat gaagcaggat gaggtactgg
      961 gaggttttga tcctgcgaat tatgaatcga agcgattatg ggcaagggct gccgacagca
     1021 caatgatacc catttcgctg gtgtacagga aaggatttac tagaaatggc aaagcgccat
     1081 tactgcttta tggctatggg gcttacggga attcaaccga accctcattc aggcctaata
     1141 tcatcagtct gctcgataga ggttttgtgt ttgccatagc acatgtaagg ggaggtagcg
     1201 aaatgggaag atactggtac gaagatggaa aacttttaaa gaagaaaaac acattcactg
     1261 attttaatga ttgtgcacaa tatcttatta atgagaaata tactagtact gacaggctct
     1321 ttgcccaggg tggaagtgcg ggtggcctgt tgataggtgc cgtaataaac atgcgccccg
     1381 atttgtacaa aggtgtgatt gcagctgtac catttgtcga tattgttacc acgatgcttg
     1441 acccgacaat accccttact acatttgaat gggacgagtg gggagatccc agaaagaaag
     1501 agtactacga ttatatgctc tcatattcac cttacgatca ggttaaaaag caaaaatacc
     1561 cgaacattat agtaacgaca ggtttctggg actcgcaggt tcagtactgg gaaccggtaa
     1621 aatgggtggc aaaactcagg gaaatgaata ccggtaacag tctgatagtt atggatataa
     1681 atatgacggc aggtcatagc ggtgcttcag ggcgtttcga aagaaaccgt gttactgccc
     1741 tgcagtatgc cttcattctt gacctggcag gaataaggga ataaaatatc tcaaatttta
     1801 tgatggggcg atttttactt ttttggactt taattttttt tgcatcatgc gccaatcaat
     1861 ctcataatct gctggttgag gcggaaagct ttgaagatac aggcggttgg gttgtcgatc
     1921 cccagttcac cgagcagatg ggttcaccat atttgctagc ccatggactc gggaatcctg
     1981 tgaaaaatgc ttcaacaagg atttctctac cctctcctgg cagatattat atatgggtaa
     2041 gaacaagaga ctgggcacca ggtaaatggg atgctccggg aagatttaaa ctttcagtaa
     2101 acggaaaaga actgaaaaga acactgggaa ccggtaaagt tacctggtca tggcaatatg
     2161 ccggaaaagt aaagattaaa actccggaag caaggattga actgagggac ttaacaggct
     2221 ttgacggaag atgcgatgct gtttatttga gtacaataat ggaacctccg cctgaagagc
     2281 tggaagaact taatttctgg agaatgagga taaaagggga agatccttca aaagccgaaa
     2341 aattgtatta tgatctggtt gttgttggcg gtggaattgc agggtgtgct g
//
LOCUS       DAOSNB010000327         2349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_45419_length_2349_cov_3.326504, whole genome shotgun sequence.
ACCESSION   DAOSNB010000327 DAOSNB010000000
VERSION     DAOSNB010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45419_length_2349_cov_3.326504"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..2303
                     /locus_tag="PLG42_10625"
     CDS             99..2303
                     /locus_tag="PLG42_10625"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOU31704.1"
                     /translation="MNGKLFFSQIIKRNGDVVPFNREKIENAVFKATRAIGRPDRAKA
                     NRLSADVLEKLSKLEIRELPTVELVQDQVERTLFEDGDFQLAKAYILYRKQRELARNA
                     QELFSNIEVVDDYLGISDWRVNESANSAYSLQGLNQHISTTITSQYWLDRIYPENISS
                     AHKQGYFHIHDLGFLSVYCVGWDLRDLLLSGFRGVNGKTQTKPARHFRTALGQIVNFF
                     YTMQGEAAGAQAFSNFDTLLAPYIRKDNLDYKQIKQCIQEFLFNMNIPTRVGFQTPFT
                     NITMDLHVPDFLKQEQIVWGGEIQEDVYGSFQNEITLLNKAFAEVMLEGDAAGSLFSF
                     PIPTYNITPDFDWDNSDYDSIWEMTAKYGIPYFSNFVNSDMKPDDVRSMCCRLRLDKR
                     ELIKRGGGLFASNPLTGSVGVVTINLSKLGYEAISENNFFEKLKNLMILARESLEIKR
                     KTIENYTEKGLYPYSKFYLRDIYKRFNCYWKNHFSTIGINGMNECCLNFLGKDIGHPE
                     SKSFAIRVMDFMRNVLEGFQEETGNIYNLEATPAESTAFRFARIDTIQYPDIITANQK
                     AYKEGAAPYYTNSTHLPVGYSDDIYEVLSLQDDLQVKYTGGTVLHVFTGDTNMPAMSA
                     KNLIRKITKAFHLPYITISPTFSICPSHGYIPGEHFSCPECNSETDVYSRIVGYMRPV
                     NQWNKGKKQEFKDRKLFRPDTGNKEMEEDQGMEENQGIPEKASLIPELNKSR"
BASE COUNT          725 a          449 c          519 g          656 t
ORIGIN      
        1 tcgtagcttt cgggcgaaaa gcaattgaat ttctgccatt tgcaggcatt cttagaatcc
       61 cccgcaggtt accattacta atcttaaatt catttaatat gaatggtaaa ctattttttt
      121 cacaaatcat taaacgtaac ggcgatgttg tacccttcaa ccgcgagaaa attgaaaatg
      181 ctgtttttaa agcaacacgc gccatcggcc gtcctgacag ggcaaaagcg aacagattat
      241 cggcagatgt tttggaaaaa ctgtcaaaat tagaaatccg ggaattacct acagtggaac
      301 ttgtacagga ccaggttgaa aggacattgt ttgaagatgg tgattttcaa ctggcaaaag
      361 cttatatcct gtacagaaaa caaagggagc tggcaagaaa tgcacaggaa ttattttcca
      421 acattgaagt tgttgacgat tatcttggta tatctgactg gagggtcaat gaaagtgcca
      481 attcagcata ttcccttcag gggctgaacc agcatatctc cactacaatc acttcacagt
      541 actggcttga caggatctat cctgagaata tctcctcagc ccataagcag gggtatttcc
      601 atattcatga cctcgggttt ctgagtgttt attgtgtcgg ctgggacctg agggacctgc
      661 ttctgtcagg attcaggggt gttaacggga agacgcagac aaaaccggca agacatttca
      721 ggactgccct cggacaaatt gtaaatttct tttatacgat gcagggtgag gctgcgggag
      781 cacaggcttt ctcaaatttc gataccttgc ttgctccata tatcaggaag gataatctgg
      841 attataagca aattaagcaa tgcatccagg aatttctctt taatatgaac atacctacaa
      901 gagtaggttt tcagacccct tttacaaata ttacaatgga tcttcatgtt ccggattttc
      961 ttaaacagga acagattgta tggggtggtg agatacagga agatgtatat ggcagttttc
     1021 aaaacgaaat taccctgctg aacaaggcct ttgcagaagt aatgctcgaa ggtgatgcag
     1081 cgggaagtct gttctctttt ccaataccaa cctataatat tacaccggat tttgactggg
     1141 ataattctga ttacgattca atttgggaaa tgactgcaaa atatggcata ccctattttt
     1201 ccaattttgt gaattctgac atgaagcctg atgatgtaag aagcatgtgt tgccgtcttc
     1261 gtcttgataa aagagaatta atcaagcgcg gaggcggatt atttgcttcc aatcctctta
     1321 cgggttctgt tggtgtggta actattaatc tttccaaact cggctacgaa gcaatatcag
     1381 agaataactt ttttgaaaag cttaaaaacc tgatgattct tgcacgggag agcctcgaga
     1441 taaaacgtaa aacaattgag aattatacag aaaaaggcct gtatccatat tcaaaatttt
     1501 acttgcgtga tatttataaa agatttaact gctattggaa aaaccacttt tcaactatcg
     1561 gaataaacgg aatgaacgaa tgttgtctta atttcctcgg aaaagatatt ggtcatccgg
     1621 aaagcaagtc ttttgccatc agggtaatgg attttatgag aaatgtgctg gaaggatttc
     1681 aggaagaaac cggcaatatt tataatctcg aagctactcc ggctgaaagc actgcattca
     1741 ggtttgcacg gattgacaca atacagtatc cggatataat cactgcaaac cagaaggcat
     1801 ataaggaagg agctgcaccc tactacacaa actcaactca tttgccggtc ggttattctg
     1861 atgatattta tgaagtatta tcgttgcagg atgatcttca ggtgaaatat accggtggta
     1921 cggttcttca tgtttttacc ggggatacaa atatgccggc catgtctgca aaaaacctga
     1981 tcaggaaaat aacaaaggct tttcaccttc cttatataac catctctccg actttcagca
     2041 tctgtccctc acatggctat attccgggag aacacttcag ctgccctgag tgtaattcgg
     2101 aaactgacgt atatagcagg attgtcgggt atatgaggcc ggttaaccaa tggaacaaag
     2161 ggaagaagca ggaatttaag gacaggaaac tgttcaggcc ggacaccggt aataaggaaa
     2221 tggaggaaga tcagggaatg gaggaaaatc agggaatccc tgaaaaggcg tctctgattc
     2281 cggaattaaa taaatcccgc taatgcgtat cggcgggttt cttaaacata gtctgattga
     2341 ctgggaggg
//
LOCUS       DAOSNB010000328         2330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_45910_length_2330_cov_4.028571, whole genome shotgun sequence.
ACCESSION   DAOSNB010000328 DAOSNB010000000
VERSION     DAOSNB010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45910_length_2330_cov_4.028571"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PLG42_10630"
     CDS             <1..987
                     /locus_tag="PLG42_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOU31705.1"
                     /translation="ILLDNKLPGIQGVDVLDYIRNNNLDIVVAMITSYASLDVAVKAT
                     HSGATDFIPKPFTPQELKSSMEHITKQLYLKRITHRLNEEGKKVRYQFLSVLSHELKA
                     PLNAIEGYLVMMKEKQLGDKIDDYAVPIERALRRIQGMRTLIMDLLDFTKIKLQRRED
                     RLQPVDLAEIASDSIATVRPYAIQKEVEIKLDKKSDATVLADPGDMAIIFNNLISNAV
                     KYNVEGGKVEIILDSTDDEVIAVFADTGIGIRPEDKDNLFTEFVRIKREEIKDATGSG
                     LGLSIVKKVTDLYKGTISVESEIGKGSIFTVRLPKNKSGFNDAKHEITGKNS"
     gene            962..1594
                     /locus_tag="PLG42_10635"
     CDS             962..1594
                     /locus_tag="PLG42_10635"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOU31706.1"
                     /translation="MKLPGKTVERLSEYRRILLNLLAEKKNYIYSYELAALLHITAVQ
                     VRRDLMLIGYSSGARKGYDIRELVETIGRIIDSEEGLNVAIVGMGNLGRAVASYFRGK
                     RSKLNLVASFDNDPQKINKVISGVKCFDIKTMEQTIRELNISIAILTVPPDAAKNTCD
                     EIVRYGIRGILNFTTVSLKVPPDVYLEEYDMITSIEKVAYFVKENLKTQG"
     gene            1598..>2330
                     /locus_tag="PLG42_10640"
     CDS             1598..>2330
                     /locus_tag="PLG42_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOU31707.1"
                     /translation="MKVFRGFEGVGRFNNPVVTTGSFDGVHIGHKAIINRLKRHASII
                     KGETLLITFYPHPRKVLYPETEGKNLRLITTLEEKIYLLEKTGLDNVIIVEFTPEFAK
                     TTCEEFVKKYLVDTIGARVVVVGFNHYFGWHRQGDFHFLQELGRKYGFEAEEIPEQEI
                     EHEAVSSTRIRKAISEGYIQRANAYLESFYMITGQLTETKEFELTGHNFFRMHIYDKE
                     DKLVPPPGIYATSIEKEKRKVRGLVL"
BASE COUNT          803 a          433 c          526 g          568 t
ORIGIN      
        1 attcttctag ataataaact gcccggaatt cagggtgtgg atgtgctgga ttatatccgc
       61 aacaacaacc ttgatattgt agtagcaatg ataacgtcgt atgcttctct cgacgtcgcg
      121 gtaaaagcca ctcacagcgg agccaccgat ttcataccca aaccattcac cccgcaggag
      181 cttaaatcga gtatggaaca tatcacaaaa caattatacc ttaaaagaat aactcaccgg
      241 ctcaacgagg aagggaagaa agtcagatac cagttcctct cagtcctgtc gcatgaactg
      301 aaagcacccc ttaatgcaat tgaaggctac ctggtgatga tgaaggaaaa acagcttggc
      361 gataaaatag atgattatgc agtaccaata gagagagccc tgagaaggat tcagggaatg
      421 aggacgctta ttatggatct gctcgatttt acaaagataa aacttcaaag aagggaagac
      481 agattgcaac ctgtcgacct tgcagagata gcatccgatt caatagcaac cgttaggcct
      541 tacgccatac agaaagaggt tgagattaaa ctggataaaa aatccgatgc aacagtcctg
      601 gcagatcctg gtgacatggc aataattttc aataacctga tttccaatgc tgtaaaatat
      661 aatgtcgagg gcgggaaggt tgaaataatt ctggatagta cagatgacga ggtgatagca
      721 gtgttcgcag atacaggcat cggaataaga cctgaagata aggacaacct ttttactgag
      781 tttgtcagaa tcaagaggga agaaataaaa gatgcaactg gcagcgggct gggactttca
      841 atagtaaaaa aagtaacaga tctttataaa ggcactataa gcgttgaaag tgagataggc
      901 aaaggaagta tatttacagt gagattacca aaaaataaat ctggattcaa tgatgcaaaa
      961 catgaaatta ccgggaaaaa cagttgagag gctcagcgag tacagaagaa ttctgctgaa
     1021 tctgcttgct gaaaagaaga attatatcta ttcgtacgaa ctggcagcac tgcttcacat
     1081 taccgctgtt caggtaaggc gcgaccttat gctgataggc tattctagcg gggcaaggaa
     1141 aggttacgac ataagggaac tggtcgaaac aataggaaga ataatagaca gtgaggaagg
     1201 acttaatgtg gcaatagtgg gtatgggcaa cctcggaaga gcagttgctt cttatttcag
     1261 agggaaacgc tccaaactga accttgtggc ttcatttgac aacgacccgc agaaaatcaa
     1321 taaggtaatc tcaggcgtga aatgtttcga tatcaaaacg atggaacaaa ctataagaga
     1381 actgaacatt tcaatagcaa tccttactgt tcctcccgat gcagcaaaaa atacttgcga
     1441 tgagattgtc agatacggta tacgtggaat cctcaatttt acaacagtgt cccttaaggt
     1501 tccgcctgat gtttaccttg aagagtacga tatgataaca tcaattgaaa aagttgccta
     1561 ttttgttaag gaaaatctga aaacccaggg ttaagagatg aaagttttca gaggctttga
     1621 aggtgtcggt agattcaata acccggttgt cacaaccggt tcgttcgacg gtgtacatat
     1681 cggacataaa gcaataatca accggctcaa gcgccatgca agcatcataa aaggtgaaac
     1741 attgcttatt acattctacc cgcacccgcg aaaagtactt tacccggaaa ctgaagggaa
     1801 aaacctccgg ctcattacaa cacttgaaga gaaaatctat cttcttgaaa agacagggct
     1861 cgacaatgta attattgtag aatttactcc tgagtttgca aaaaccacct gtgaagagtt
     1921 cgtaaaaaaa tatctggttg atacaattgg tgcaagagtt gttgttgtgg gtttcaacca
     1981 ctacttcggc tggcacagac agggtgattt ccattttttg caggaactgg gcaggaaata
     2041 tggatttgaa gcagaagaga tacccgaaca ggagatagaa catgaagcag taagttccac
     2101 acgtatcaga aaagctatta gcgaaggtta tatacaacgc gctaatgctt atctcgaaag
     2161 cttctatatg attacgggac aattgactga aacaaaagaa tttgaattaa caggtcataa
     2221 tttcttcagg atgcatattt atgataagga agacaaactt gtccctcctc ccggaattta
     2281 tgcaacaagc attgagaagg aaaagagaaa agtcagaggt ctggttctta
//
LOCUS       DAOSNB010000329         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_48389_length_2241_cov_4.686185, whole genome shotgun sequence.
ACCESSION   DAOSNB010000329 DAOSNB010000000
VERSION     DAOSNB010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48389_length_2241_cov_4.686185"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1090)
                     /gene="trpS"
                     /locus_tag="PLG42_10645"
     CDS             complement(98..1090)
                     /gene="trpS"
                     /locus_tag="PLG42_10645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOU31708.1"
                     /translation="MDIVVSGIRSTGKLHLGNYFGAIRNFVRMQRENKCFFFIADYHS
                     LTTHPKPDDLHGNIKQVLAEFLACGIDPEVATVFIQSDIPEVPELYLLLNMNAYVGEL
                     QRTTSFKEKVRKQPDNVNAGLLTYPVLMAADIIIHKATKVPVGKDQEQHLEMTRRFAR
                     RFNTMYGVNYFPEPDAYNFGEKLIKIPGLDGSGKMGKSDDNGIYLSDSPEEIRKKVMK
                     AVTDAGPTVPNQKMSVPIKNLFTIMSVVSDPATVKYYKEKYATCEIRYGEMKKQLAED
                     IIKFVTPIRERIIQISSDTKYLAKVVAEGAEKARASASATLRDVKEIIGYRKWI"
     gene            1227..1565
                     /gene="rbfA"
                     /locus_tag="PLG42_10650"
     CDS             1227..1565
                     /gene="rbfA"
                     /locus_tag="PLG42_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOU31709.1"
                     /translation="MESTRQKKVSRLIQKEIATILQRKTNELAPGKLISVTVVRMSPD
                     LSLAKVYLSIFPSAEKQEVLNTLKDHSSRIRFELGQKIRTQLRIVPEIAYFIDDSLDY
                     IENIEKLLKH"
     gene            1570..>2241
                     /locus_tag="PLG42_10655"
     CDS             1570..>2241
                     /locus_tag="PLG42_10655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOU31710.1"
                     /translation="MHFEPFKHLAGKIVKKSPLLRKVFYFTLGIIFLRSWHFRRALKK
                     TGQNLPPDASVLDAGSGMGQHTWWMARNFRGWKIKGIDIISEQVNDCNEFFRRSGMGN
                     RISFEVADLVNYSEENQYDLIISVDVIEHIENDIQVFKNFWSSLKNGGVLLITTPSDR
                     GGSGITSAGNPSFIDEHVRNGYSTEEITDKLKSAGFSNIETTYTYGKPGHVSWLISVK
                     YPAMLL"
BASE COUNT          669 a          463 c          428 g          681 t
ORIGIN      
        1 actttggcac atattttgtt cataaattgt tgcaggcaat ctccatcata tacttgattc
       61 ttcagggcag ccaggtcaga tacctggctt taatatttta tatccatttg cggtaaccta
      121 taatttcctt tacatctctc aaagttgcag aagcacttgc tcttgctttt tcagcaccct
      181 ctgctacaac ctttgcaaga tacttcgtgt cggatgatat ctgaattatc ctttccctta
      241 ttggagttac gaatttaatt atgtcttcag ccagctgctt tttcatttcc ccatacctga
      301 tctcacatgt agcatatttt tccttataat attttactgt agcagggtca gatacaacgc
      361 tcataattgt aaagaggttc ttaatcggaa ccgacatttt ctggttagga acagttggtc
      421 ctgcatcggt aacggctttc atcacctttt ttcttatttc ttccggggaa tctgaaagat
      481 atattccatt gtcatcgctt ttacccatct tgcctgagcc atccagcccg ggtatcttaa
      541 taagtttctc tccaaaatta taagcatcgg gttcagggaa atagtttact ccgtacattg
      601 tattgaaacg gcgggcaaac ctgcgcgtca tttcaaggtg ctgttcctga tctttcccaa
      661 ccgggacttt tgtggctttg tgtataataa tatcagctgc cataagtaca ggataagtaa
      721 gcaggcctgc attaacatta tctggctgtt ttcttacctt ttccttaaaa gaagtggttc
      781 gctgcagctc cccaacataa gcattcatgt tgagcaaaag gtaaagttcc ggaacctcag
      841 gaatatcgct ctgaataaag actgtagcca cctcaggatc tattccgcag gcaagaaatt
      901 ctgccagaac ctgtttaata ttcccatgaa gatcatcagg tttagggtgt gttgtaagag
      961 agtgataatc agcaataaag aagaaacact tgttttccct ctgcatcctg acaaaatttc
     1021 tgattgcacc gaaataattt ccaaggtgca gtttccctgt tgatcttatt ccactaacaa
     1081 ctatatccat atcactttta aaatatctcg caaacctatt cttttttttc ttatttccaa
     1141 tggtgcaaat ttacttacat ttctggtcac atcgaatatc tattgaataa gtatttgtac
     1201 ctttgaacgt ttaaattacg cccgatatgg aatcgaccag gcaaaaaaag gtttcaaggc
     1261 tgattcagaa agaaatagcc actattttgc agagaaaaac aaatgaactg gcaccgggca
     1321 aacttatatc ggtaacagtt gtaagaatga gcccggatct ttctctggca aaagtgtatc
     1381 ttagcatatt tccttctgcc gaaaagcagg aagtactgaa cacccttaaa gatcattcat
     1441 cacggatacg tttcgaactg ggacaaaaaa ttagaaccca gctcagaata gtaccggaaa
     1501 ttgcttattt tattgatgac agcctcgact acatcgaaaa cattgaaaag ttactgaaac
     1561 actgatagca tgcattttga accctttaaa cacctggctg gtaaaatagt aaaaaaatcg
     1621 ccattgctca gaaaggtgtt ttacttcact cttggaatta tttttctccg gtcgtggcat
     1681 tttcgcaggg cgttaaagaa aacaggacaa aaccttcctc cggatgcttc agtactcgat
     1741 gcaggttcgg gaatgggcca gcatacatgg tggatggcaa gaaatttcag gggatggaaa
     1801 ataaaaggaa ttgacataat cagtgaacag gttaatgact gtaatgaatt tttcaggcgc
     1861 tcaggaatgg ggaacagaat cagttttgaa gttgctgacc ttgttaatta ctcggaagaa
     1921 aaccagtacg acctgattat atcggttgat gtgattgagc atatcgaaaa tgatattcag
     1981 gtcttcaaaa atttctggag ctcgctcaaa aatggaggag tattgcttat tacaacacct
     2041 tccgacagag gcggctccgg aataacttca gccggaaacc cgtcttttat agacgagcat
     2101 gtaagaaacg gctatagcac agaagaaata acagataaac ttaaatcggc aggtttcagt
     2161 aacattgaaa caacttatac atacggaaaa cccggccatg tatcgtggct gatatcagta
     2221 aagtaccctg ctatgttact t
//
LOCUS       DAOSNB010000330         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_51249_length_2150_cov_4.089737, whole genome shotgun sequence.
ACCESSION   DAOSNB010000330 DAOSNB010000000
VERSION     DAOSNB010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51249_length_2150_cov_4.089737"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="PLG42_10660"
     CDS             complement(<1..1787)
                     /locus_tag="PLG42_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31711.1"
                     /translation="MKRVFLLFLLMGSILNLSAQIKGELKDNFYWGETFILYEEYKDA
                     LPLYQLLLKVNPRNSNYKYRIGQCLLNIPGRKHEAISYLEEAVKDINPKYKEGKFKET
                     KAPFDAYYYLANAYRINNQLEKAIETYELFKKYCNTKVYDTTIVNMQIEACQNALELM
                     KVPLYVKKANLGRPVNEQYPEMNPVVSDDETIMVFNRSEPFQEALYFTKKVNGRWSEP
                     VNIIPYLGLGFEDKNYATSLSSDGRELYIYRAGANYDGNIYVTRRRDDDTWTNLIRLN
                     DNINTKYWESHAAITHDGRRLYFTSNRKGSYGGLDIYFSDRDSTGDWGVAKNIGPVIN
                     TKFNEESPFLGKDDKTLFFSSQGHFNIGGYDIFYSTLLDNGEWSVPLNVGYPLNTTDD
                     DIFFHPVKEGYEAYYSLIEPDGVGSTDIYRIEIFSKDHPRKFYVRGIVKVKDLLALFS
                     DSIRVTAVSKANPEDKVIVYTNPKTGEYIFEIPQGEYTITYEAKNAGVVSRQIEISLF
                     SPSDSILIQDIELPKLDFISELEVRSDKNILYIKGDTIEIPIKAEPESILEVEHWIGD
                     SLVRTDKYILTDSLFVYRMLPEEGTNKLV"
     gene            complement(1969..>2150)
                     /locus_tag="PLG42_10665"
     CDS             complement(1969..>2150)
                     /locus_tag="PLG42_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31712.1"
                     /translation="ILNTHRIKVIFFSLRDIPPSQLISLIEKIAAFKIIIRLVPEGEN
                     VIMGSKTIIAKDDVH"
BASE COUNT          581 a          420 c          391 g          758 t
ORIGIN      
        1 aatacgagct tatttgtgcc ttcttctgga agcatcctgt aaacgaaaag tgaatcggtt
       61 aatatgtatt tatctgttct caccagcgag tccccaatcc agtgctcaac ctccagaatt
      121 gattcaggct ccgcctttat tggtatttcg atggtatcgc cttttatgta tagtatgttt
      181 ttatcgcttc taacctcaag ttcagaaatg aagtccagtt tgggcagttc gatgtcctga
      241 attaatatac tatcggaagg actgaacagg gaaatctcaa tctgtctgga tactacacca
      301 gcattttttg cctcataagt gatagtatat tctccctgag gtatctcaaa aatatattct
      361 ccggttttag gattggtgta aacaataact ttatcttcag gatttgcttt gcttacggca
      421 gtaactctga tgctgtcgct gaaaagggca agaaggtctt ttactttaac tattcctcta
      481 acatagaatt tccttggatg gtctttggag aatatttcaa ttcggtaaat gtcggtagaa
      541 ccaactccat caggttctat cagtgaataa tacgcttcat agccttcttt tacagggtgg
      601 aagaaaatat catcgtcggt tgtattgagc gggtacccca cattgagtgg tacactccat
      661 tcgccgttgt caagcaacgt tgaatagaaa atatcatatc cgcctatatt gaaatgcccc
      721 tgggaactga agaagagagt tttgtcgtct ttacccagaa agggtgattc ttcattgaat
      781 tttgtgttaa ttacagggcc aatattcttt gccactcccc aatcgcccgt actgtcgcga
      841 tcagagaaat aaatatcgag tcccccatac gaaccctttc tgtttgatgt gaaatagagc
      901 cttctaccgt catgagtgat tgcagcatga gactcccagt attttgtatt tatgttatca
      961 ttaagcctta tcaggttggt ccatgtgtca tcatcccttc tgcgtgtaac gtatatattt
     1021 ccgtcgtaat ttgctcctgc ccggtatata taaagttctc ttccatcaga agaaagagag
     1081 gtggcatagt ttttatcttc gaatccaagc cccagatagg ggattatatt tacaggttca
     1141 ctccacctgc cattaacttt cttcgtaaaa taaagagcct cctgaaatgg ctcagacctg
     1201 ttgaatacca ttattgtttc gtcatctgac actaccgggt tcatttcagg atactgctcg
     1261 ttcaccggtc gtccgaggtt tgctttcttg acatagagcg gaactttcat aagttccaga
     1321 gcgttctggc atgcttctat ctgcatgttt actatagtgg tatcataaac tttggtgttg
     1381 cagtatttct tgaataattc ataggtttca attgctttct ccagctggtt gtttatgcga
     1441 taagcatttg ccagataata ataagcatca aacggagctt ttgtttcctt gaatttgcct
     1501 tccttgtatt ttggattgat gtcttttact gcctcttcca ggtaacttat tgcttcatgc
     1561 tttctgccag gtatattgag aaggcattgc cctatgcgat atttataatt gctgtttctg
     1621 gggttaactt tcagtaagag ctgatacagt ggtaatgcat ctttgtattc ttcataaaga
     1681 ataaaagttt cgccccagta aaagttgtct tttaattctc ctttaatctg ggcagacagg
     1741 ttcagtattg aacccatcag caaaaacaac aggaaaacac gtttcatgca aaaaatattt
     1801 tgttaaaatt aatagactat accggccgat tccagttctt ttacttaaat tattcaaatt
     1861 tatacatatt tcattacaaa atattcctgc tcctaaattt agtattaaat taaattgatt
     1921 tttcaacagt tatcggattt atttcatttt tttttaactt tttaattttc aatgaacatc
     1981 atctttggca attattgttt tgctacccat gatcacattt tcaccttccg gtaccagtct
     2041 aattattatt ttaaatgcgg ctattttttc aataagggaa ataagctgag aaggagggat
     2101 atctctcagt gaaaaaaata ttactttgat ccggtgtgtg tttaatattt
//
LOCUS       DAOSNB010000331         2098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_52960_length_2098_cov_4.131669, whole genome shotgun sequence.
ACCESSION   DAOSNB010000331 DAOSNB010000000
VERSION     DAOSNB010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52960_length_2098_cov_4.131669"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..1671)
                     /gene="xylA"
                     /locus_tag="PLG42_10670"
     CDS             complement(352..1671)
                     /gene="xylA"
                     /locus_tag="PLG42_10670"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOU31713.1"
                     /translation="MAKVPKKEFYPGIPKIEYEGVNSKNPLAFRWYNPEAKVGGKKMK
                     DHLRFAVAFWHTFCGDGSDPFGNATRFYPWDNLPLDDKIKQRLEAAFEFFTKIGVEYY
                     CFHDVDVVGNGTVFEIEKRLEKYVPLMKTLQQQTGVKLLWGTANLFSHPRYMNGAATN
                     PDFAVVACAGAQIKNAIDATIELEGQNYLFWGGREGYMSLHNTDMKRELYHMGMMLSM
                     ARDYGRKRGFKGAFFVEPKPMEPSKHQYDYDAATVIGFLRNHRLERDFKLNIEVNHST
                     LAGHSFTHELQVAADAGMLGSIDANKGDYQNGWDTDEFPTNIYEVTEAMMIILNAGGF
                     TTGGINFDAKVRRNSTDPEDLFIAHISGMDTFARALIIADKILRESDYLKMKKKRYES
                     FNKGKGKEFERGKLTLEDLREIAKELGEPAPKSGKQELLEQLINMYI"
     gene            complement(1741..>2098)
                     /locus_tag="PLG42_10675"
     CDS             complement(1741..>2098)
                     /locus_tag="PLG42_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOU31714.1"
                     /translation="FRASQEGIAFSFRYGLDIMKETGIEAGIMRAGAANMFLSPVFRE
                     VLSCVTGSVIHLYNTDGSLGAARGAGIGCGYYKTEKEAFAGLKSVGVTEPDKKISGTY
                     QEIYEKWKEIVVEAIK"
BASE COUNT          522 a          474 c          395 g          707 t
ORIGIN      
        1 aactttacga cctggctgcc aattttgcct tcattataat aatgaatgtc ggaaccgcaa
       61 acacctaccg accttattct tataagaacc tcatcagact ggctgatttc aggaactggc
      121 ttttcggtaa tttcaattct cctgatgccg gtgagaatca ttgctttcat agtcaaaaaa
      181 catatatcat ctttgtgatc ccttcgtgaa ttttttgtgg tcattacgca taaaaacact
      241 tctttccaca gatggcacgg agaatttcac gaagagtcac aaagtcaaca atttattttt
      301 attataagca ttaaaaaata tgcagtttaa gaaagtcaat cgattaatcg attaaatata
      361 catattaatc agttgttcaa ggagttcctg cttaccactt ttcggagctg gttctccaag
      421 ttcttttgca atctccctga ggtcttcgag cgttagttta cctctctcga attcttttcc
      481 tttaccctta ttgaatgact catatctctt ctttttcatt ttgaggtagt cgctctccct
      541 gagtatttta tctgcaatta taagtgctct tgcaaaggta tccataccgc ttatatgggc
      601 tatgaagagg tcttcggggt ctgttgagtt gcggcgcact ttggcatcga agtttatgcc
      661 tccggtagta aagccaccgg cattaagtat aatcatcatt gcctcagtta cttcatagat
      721 atttgtcgga aactcatcag tgtcccatcc gttctgatag tcgcccttgt ttgcgtcaat
      781 acttccaagc ataccggcat cagcggctac ctgcagttca tgtgtaaagc tgtgtccggc
      841 aagtgttgag tggttcactt cgatattaag tttaaaatcc ctctcgaggc ggtggtttct
      901 caggaagcca ataacagttg cagcatcgta gtcgtactgg tgttttgagg gttccattgg
      961 ttttggttca acaaagaatg cacctttgaa acctctcttg cgtccataat cgcgtgccat
     1021 ggagagcatc atgcccatat ggtaaagttc tcgtttcatg tcggtgttat ggagactcat
     1081 atatccttcc ctgcctcccc agaacaaata gttttgtcct tcaagttcaa ttgtagcgtc
     1141 aattgcattt ttaatctgtg ctcctgcaca tgccacaacc gcaaaatcag gatttgttgc
     1201 tgcaccgttc atataacggg gatgtgaaaa gaggtttgct gttccccata gaagtttaac
     1261 tccggtctgc tgctgcagtg ttttcatcag aggaacatat ttctcgagac gtttctctat
     1321 ttcaaacact gtcccgttac ctacaacatc aacatcatga aaacagtaat attcaacgcc
     1381 tatcttggta aagaattcga atgctgcctc gagtctttgt ttaatcttat catcgagtgg
     1441 aaggttatcc catggataga atctggtagc attaccgaag ggatcacttc cgtcgccgca
     1501 gaaggtgtgc cagaatgcca cagcaaacct caggtggtct ttcatttttt ttccgccaac
     1561 cttagcttca ggattatacc atctgaatgc cagcggattt tttgaattta ctccctcata
     1621 ctctatcttt ggtatgccgg ggtagaattc ttttttcgga acttttgcca tagtgtataa
     1681 aattttagat tatttattat ttttacttat tatattttac tgattttcag attaatatga
     1741 ctacttaata gcttcaacca ctatttcttt ccatttttca tatatctcct gatatgttcc
     1801 ggagattttt ttatctggtt ctgtcacgcc caccgatttg agtcctgcga aggcttcttt
     1861 ttcagtttta tagtagccgc agcctattcc agctcccctg gcggcgccca gtgaaccgtc
     1921 ggtgttataa agatgtatca ctgaacccgt tacacaggac agtacttccc tgaatacagg
     1981 actgaggaac atgtttgctg ctcctgcacg cattattcct gcttcaatgc ccgtttcctt
     2041 catgatatcg agtccatacc tgaatgagaa agctattcct tcctgtgatg ctctgaaa
//
LOCUS       DAOSNB010000332         1999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_56564_length_1999_cov_3.896605, whole genome shotgun sequence.
ACCESSION   DAOSNB010000332 DAOSNB010000000
VERSION     DAOSNB010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56564_length_1999_cov_3.896605"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1999)
                     /locus_tag="PLG42_10680"
     CDS             complement(<1..>1999)
                     /locus_tag="PLG42_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOU31715.1"
                     /translation="LPKDMAAELYKPFIIRKLIERGIVKTVKSAKKIVDRRDPVVWDI
                     LENVLKGHPVLLNRAPTLHRLGIQAFQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVH
                     VPLGNTAILEAQILMLASHNILNPANGAPITVPSQDMVLGLYYMTKGKKSSETEKVKG
                     EGLVFYSPEEVIIAHNEGKADLHAWIKVKVDDIVDGKPVKRLIETTVGRVLFNQCVPK
                     EMGFINELLTKKSLREIIGKVLKVAGIAKTAEFLDSIKEMGFMMAFKGGLSFNLDDVI
                     IPEEKIRIVEEGYEQVEEVLNNYNMGFITNNERYNQIIDIWTHVNARLTQALLQQLSA
                     DKQGFNSIYMMLDSGARGSKEQIRQLSGMRGLMAKPQKSGATGSEIIENPILSNFKEG
                     LSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVIINEEDCGTLRGLVATA
                     IKKNEEVVETLYERILGRTAVHDVFHPLTGELIVAAGEEITEEIASVIEDSPIEQVEI
                     RSVLTCESKKGVCAKCYGRNLATGHMVQIGEAVGVIAAQSIGEPGTQLTLRTFHVGGT
                     ASNITTESSLVAKYSGIAEFEELKTVSKKDPEKGEIDVVIGRLAELRIVDRKTGIALT
                     THSVPYGSKLYIKPGQEVEKGTLICEWDPFNAVIVSEVSGKV"
BASE COUNT          478 a          484 c          411 g          626 t
ORIGIN      
        1 aaccttacct gatacttctg atacaataac tgcattaaac ggatcccatt cgcagataag
       61 ggttcccttt tcaacttcct gtccaggttt gatgtaaagt ttggagccgt aaggaacaga
      121 atgtgttgta agggctatgc cggtctttct atctactatt ctgagttcgg caagacgtcc
      181 aattacaaca tcaatttctc ccttttccgg gtcctttttg gatactgttt taagttcttc
      241 gaattctgca ataccgctgt attttgcaac caggctcgat tcagtggtta tatttgaagc
      301 cgtaccacca acgtggaaag tacgcagggt aagctgtgta ccgggttcgc cgatactctg
      361 agcggctatt acgcctactg cttctcctat ctggaccatg tggcctgttg caaggtttcg
      421 tccgtagcat tttgcgcaga cacccttctt tgactcacag gtcagaaccg accttatctc
      481 cacctgttca attggtgaat cctctataac cgatgcaatt tcctcggtaa tctcttcacc
      541 ggctgccact atcagttcac ctgtaagggg atggaacaca tcatgtactg cggttcttcc
      601 aaggattctc tcatacagtg tttcaacaac ttcctcattt ttcttaatag ccgttgctac
      661 aagtcccctc agtgttccgc aatcctcttc gtttatgata acatcctgtg ctacgtcaac
      721 aagtcttctg gttaggtaac ctgcatctgc cgtcttgaga gcagtatcgg caagaccttt
      781 acgggcaccg tgtgtggaaa taaagtattc gagaaccgac aaaccttcct tgaagttcga
      841 aaggatcggg ttttcgatga tctcagagcc tgttgcgccc gatttctggg gtttggccat
      901 aagtcccctc atacccgaga gctgacgtat ctgctccttc gaacctcttg cccctgaatc
      961 gagcatcata tagattgaat tgaatccttg cttgtcggct gatagctgct gtaaaagcgc
     1021 ctgtgtaagc cttgcattaa catgagtcca gatatcaata atctggttgt aacgttcatt
     1081 attagtgatg aaccccatgt tatagttatt cagtacttcc tcaacctgct cgtaaccctc
     1141 ctcaactatt ctgatcttct cttccggaat gatcacatca tcgaggttaa atgagagtcc
     1201 acccttgaat gccatcataa atcccatctc cttgatggag tcaaggaatt ctgcagtttt
     1261 ggctatacct gcaactttaa gaacttttcc gatgatttcg cgaagcgatt tcttggtaag
     1321 aagttcgttt atgaaaccca tctcttttgg aacacactgg ttgaagagta cccttcctac
     1381 agtggtttca atgagtcgtt ttacaggctt gccgtcaact atatcatcta cttttacctt
     1441 tatccatgca tgaagatctg cctttccttc attatgggca atgattacct cttcaggtga
     1501 atagaacacc aggccttctc ccttaacttt ttctgtctcg gagcttttct ttccttttgt
     1561 catgtagtaa agtccgagaa ccatatcctg agaaggcacg gttatgggag caccgtttgc
     1621 agggtttaga atgttatgtg aagccagcat taatatctgg gcttcgagta tggcagtatt
     1681 tccaagtggc acgtgaacgg ccatctggtc accgtcgaag tcggcattga atgccgtaca
     1741 aaccagcggg tgaagctgta ttgcttttcc ttcgatcagc ttcggctgga aagcctgaat
     1801 acccagacgg tgcagtgtag gagcacggtt cagaagcacc gggtgtcctt taagtacatt
     1861 ttcaagaata tcccatacta cagggtctct tctgtcaact atcttcttag ctgatttaac
     1921 agtctttact ataccacgct caataagttt tctgataatg aaaggtttat aaagttcggc
     1981 tgccatgtct tttggcaaa
//
LOCUS       DAOSNB010000333         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_57348_length_1979_cov_4.076403, whole genome shotgun sequence.
ACCESSION   DAOSNB010000333 DAOSNB010000000
VERSION     DAOSNB010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57348_length_1979_cov_4.076403"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLG42_10685"
     CDS             <1..591
                     /locus_tag="PLG42_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31716.1"
                     /translation="IYYRSCLTLFIIASLLFLGVWANINNIFRITGPEYMKGKWVVFF
                     IALGSLIDMATGANSSIFGTSAYYRIQTYFLLLLVVMIIAANIILIPLLGITGAAIGS
                     AGALTLLNLMRFLFLYYKFKLQPYNIKFLYVILIGVISYIISAVIPEMKNLIADILLR
                     SSVVTLLFFIPVYLLKISEDITARTDDVLRFLKIKK"
     gene            605..1939
                     /locus_tag="PLG42_10690"
     CDS             605..1939
                     /locus_tag="PLG42_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOU31717.1"
                     /translation="MDIRFKYFLKNFQLFFNITENDVGYLPDGEAPVLIERKTNDSFW
                     SSHADINFSSVAWKEWKGVKIPFLFDNDQTKDIVTYSGNKAVINYDIVASVFYFLSGW
                     NEIVSPGRDDHQRPIFRESAINKLSLQNTPVVNYYFDILYETIKHVKKAEIKKPLWDN
                     HKFAVALTHDIDKCQSAWIEGSASELRKGRFLSVPVLLAKRFLKNKDEWFNFDEIINI
                     EKKYNATSSFFFLSRRGKSGGLENADYNIERKHLYNTIEFLKRNGFDTGIHGSPGTHV
                     NAQALKSEIKKVSPDSVWGNRFHFLLFDPIKSASVLEECGIKYDSTLGFPDYPCFRRA
                     TCYPFYLFNFEKNDISPVIEIPLTVMDVTLAHKKYLGLNHEKALLIIKNLADEVRKHG
                     GVLTLLWHNTYFSDYKFKGWREVYTGILDYCKENDALLTNARTIYERVWSKA"
BASE COUNT          648 a          353 c          374 g          604 t
ORIGIN      
        1 atatattaca gaagctgtct gactcttttc attattgctt ccctgctttt ccttggagtt
       61 tgggcaaata tcaacaatat tttcagaata accggcccgg aatatatgaa aggaaagtgg
      121 gttgtcttct ttatagcact gggaagcctg atcgacatgg caacaggagc taacagttca
      181 attttcggca cttcagctta ctacagaatt cagacatatt tcctgttact ccttgtagtt
      241 atgattattg cagccaatat tatactcata ccgttactgg gcattaccgg tgctgcaatt
      301 ggcagtgcag gcgcactgac cctgttaaac ttaatgaggt tccttttcct ctattacaaa
      361 tttaagctcc agccttataa tataaaattc ttatatgtca ttttgatagg agtaatttca
      421 tatattatat cggccgttat tccagaaatg aaaaatctga ttgccgatat tctcctgaga
      481 agttctgttg tgactctgct tttcttcata cccgtttacc ttctgaagat ttcggaagac
      541 attaccgcca gaacagatga tgtattgagg tttttgaaaa tcaaaaaata aaagacacta
      601 aataatggat atccggttta aatatttcct gaaaaatttt cagttatttt ttaatatcac
      661 tgaaaatgat gtcggatatc tgcccgatgg tgaagcccct gtacttatcg aacgcaaaac
      721 aaacgattct ttctggtcgt cacatgcaga tatcaatttc agttcagttg catggaagga
      781 atggaaagga gttaaaatcc catttttatt cgacaacgat caaactaaag atatagttac
      841 atacagcggg aacaaggctg taataaacta cgacatagta gcatctgttt tctatttcct
      901 tagcggatgg aacgagattg tatctcccgg cagagatgac catcaaagac caatcttcag
      961 agaaagtgca ataaataaat tgtcactgca gaatactccg gttgtaaatt attatttcga
     1021 catcctgtat gaaacaataa aacatgtaaa gaaagcagaa ataaagaaac ctctatggga
     1081 taatcataaa tttgcagttg cacttaccca cgatatagac aaatgccaga gtgcatggat
     1141 tgaaggctct gcaagtgaat tgagaaaagg ccgttttctg tctgttccag ttttattagc
     1201 caaaaggttc ctcaaaaata aggatgaatg gttcaatttt gatgaaataa taaatattga
     1261 aaagaagtac aatgcgactt cctctttctt ttttctgtcg cgaagaggta aaagcggcgg
     1321 actggaaaat gcagattata atattgaaag aaaacacctt tataatacga ttgaatttct
     1381 gaaaagaaat ggattcgaca caggaataca tgggagtcca ggtacacatg tcaacgctca
     1441 agctttaaaa tctgaaatta aaaaggtgtc gccggattca gtatggggta acaggtttca
     1501 ctttctactt tttgacccga taaaatcagc ctctgtgctg gaagaatgcg gaatcaaata
     1561 cgacagcact ctgggttttc ccgactatcc ctgtttcaga agggctacct gttatccgtt
     1621 ttatcttttt aattttgaaa aaaatgatat ctctccagtt attgaaatac ctcttaccgt
     1681 aatggatgtt actctggcac ataaaaaata cctgggtctg aatcatgaaa aagcattact
     1741 cataatcaaa aatctggccg atgaagtcag aaaacatgga ggtgttctta cattactctg
     1801 gcataatact tatttttcgg attataaatt taaaggatgg agggaagttt atactggaat
     1861 tctcgattat tgtaaagaaa acgatgcttt actgacaaat gcaaggacaa tttatgaaag
     1921 agtctggagc aaagcataag atacttgtac ttgacggtga tcataaaaac gcacttgcg
//
LOCUS       DAOSNB010000334         1954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_58423_length_1954_cov_5.469194, whole genome shotgun sequence.
ACCESSION   DAOSNB010000334 DAOSNB010000000
VERSION     DAOSNB010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58423_length_1954_cov_5.469194"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLG42_10695"
     CDS             <1..911
                     /locus_tag="PLG42_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31718.1"
                     /translation="SFSLLFFGVMHIDFYFVRNTTDRWISRNCSFRQRSKKPEIWDPV
                     TGDISRVMIYRESQGYTIMPLTFAPFESYFVVFKDSPGTAPYSEIKGNESEPPLLKYS
                     DNDIIILDECNFELAGRENLKFKNVIYEQEIKGAWEIFFPLGEEKPSRIIFPRLVSWT
                     ESEIEDIKYFSGIATYKKNFHYDINFLRKGEFRFFLEFEDISKVGDVWLNGKHLGIAW
                     TKPFRFEVTDALKPGNNTLVVNVANTWSNRLKGDALTGKKNTYTNIKITSIGGLDNIY
                     ATWAEVPLIKSGLIGKARIYAIKPVK"
     gene            1005..1910
                     /locus_tag="PLG42_10700"
     CDS             1005..1910
                     /locus_tag="PLG42_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOU31719.1"
                     /translation="MKTTRRIFIRNSALAAAGAMILPKSLPPVPPKAKGIVGLQLYSV
                     RAEMSKDPLGSLKQLAEMGYVYVEHANYVNGKFYGYEPKEFRKILDDLGLKMISGHTA
                     LNPNHWDDSKKDFADSWKKLVEDAAILGQQYVVSPAMNQAQYRTYDDLMKTMEIFNKA
                     GELCKKYKMKFGYHNHDFEFKVEFNGEKMFDIIMKTIDPKMVVIQLDTGNLYNGGAKA
                     LDVVKKYPKRWENIHVKDEIQTEKGTYESCILGEGIVNIQEVLSVATKVGGAEVYIIE
                     QEAYQGKSPMDCVKADLEIMRKWGY"
BASE COUNT          644 a          299 c          438 g          573 t
ORIGIN      
        1 ttagtttttc tttattgttt tttggagtca tgcatattga tttttatttt gtaagaaata
       61 caacagacag atggatctcg cggaactgca gtttcaggca gcgtagcaag aaaccagaaa
      121 tatgggatcc tgtcacagga gatatatcca gggtaatgat ttaccgggag agtcaaggtt
      181 atactatcat gcctttaaca tttgcaccct ttgagtcata ttttgttgtt tttaaagatt
      241 cacccggcac tgcaccttac agtgaaatta aaggaaatga aagcgagccc cctctcctta
      301 aatattcaga taatgatata attatacttg atgagtgtaa ttttgaactt gcagggaggg
      361 agaatttaaa gtttaaaaat gttatttacg agcaggagat taaaggggcc tgggaaatat
      421 ttttccctct gggtgaagaa aaacccagcc gcataatttt cccacggctt gtttcatgga
      481 cagagtcgga gatagaagac attaaatatt tttcaggcat agcaacctac aaaaaaaatt
      541 ttcattatga tattaatttc ttaagaaaag gtgaattcag attttttctt gaatttgaag
      601 atatttcaaa agttggagat gtatggctga atgggaagca tctggggata gcatggacaa
      661 aacctttccg ttttgaggtt acagatgctc ttaaaccagg gaacaacact ctggtagtta
      721 atgttgcaaa cacatggtcg aacaggctca aaggtgatgc cctcactggc aagaagaata
      781 catatacaaa tattaaaata acaagtattg gcggactgga caatatttat gcaacatggg
      841 ctgaagttcc tctgataaaa tcgggcctta ttggaaaagc cagaatatat gcaattaaac
      901 ctgttaaata gtttgacggt attaaactgt tactgtttaa ttatttttat aactttaggt
      961 tcattatatt attttattta ataatctttt aaatcggaag taaaatgaag acaacaagaa
     1021 gaatatttat ccgtaacagt gctcttgcag ctgcaggtgc aatgattttg ccgaagtcat
     1081 taccaccggt acctccaaag gctaaaggaa ttgtgggact tcagctttat tcggtgagag
     1141 ctgaaatgtc aaaagatcct cttggttcat tgaaacaact tgcagaaatg ggatatgtat
     1201 atgtggagca tgccaattat gtaaacggta aattttatgg ttatgaacca aaggaattca
     1261 ggaagattct tgatgacctt ggattgaaga tgataagcgg tcatacggcg ctcaatccga
     1321 atcattggga tgattcgaaa aaagactttg ctgattcatg gaagaaactt gtagaggatg
     1381 cagctatcct tgggcagcaa tatgttgtga gcccggcgat gaaccaggcc cagtaccgca
     1441 cctacgacga tcttatgaag acgatggaaa tctttaacaa ggccggtgaa ctgtgcaaaa
     1501 aatacaaaat gaagttcggc tatcacaatc atgattttga attcaaggtt gaattcaatg
     1561 gtgaaaagat gtttgatatt attatgaaaa cgatagaccc taaaatggtt gtgattcagc
     1621 tcgataccgg gaatctttat aacggtgggg caaaggcact cgatgtggtg aaaaaatatc
     1681 ctaaacggtg ggaaaacatt catgtaaaag atgagatcca gaccgaaaaa ggaacgtatg
     1741 aaagttgcat actcggagag gggattgtga atatacagga agtactttca gtggctacaa
     1801 aggttggagg ggcagaagtc tatataattg agcaggaggc ataccagggg aaatcaccca
     1861 tggattgtgt taaggctgat ctcgagatta tgagaaaatg gggttattga taagcaaaag
     1921 ttttcagttt gtaagttttc tgtattttat tcgt
//
LOCUS       DAOSNB010000335         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_64053_length_1831_cov_4.744369, whole genome shotgun sequence.
ACCESSION   DAOSNB010000335 DAOSNB010000000
VERSION     DAOSNB010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64053_length_1831_cov_4.744369"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLG42_10705"
     CDS             complement(<1..1146)
                     /locus_tag="PLG42_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31720.1"
                     /translation="MKKLLLVVALMGSFILTLNATHNRAGEITYVQLSDLTYEFTITT
                     FTYTLSYADRPQLPIEWGDGTSSIAPRIEIVMLPNYYRKNVYKIIHTFPGPGVYDVVV
                     QDPNRNYGVQNIPNSVNVVFSIRTKLVVNPAMGKNSTPVLLNPPYDKAAKGYLFIHNP
                     AAFDPDGDSLSYQLTVCTRENGKPIENYTFPPATHKFYVDSISGDLVWDTPADTGKYN
                     VAMEIQEWRNGKKIGVVVRDMQIEVYNTKNHPPVNSTLRDFCVEAGDTVDFIVTATDK
                     DDDFIRLKATSGVFSLPGCSATFSKIDSLAGYASARFFWKTCYENVRHQPYDIIIKSE
                     DNSRDLSLFDIDNFRIKVLGPAPHLLSASPEGKFIRLEWDNYGTNVIA"
     gene            complement(1153..1644)
                     /locus_tag="PLG42_10710"
     CDS             complement(1153..1644)
                     /locus_tag="PLG42_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOU31721.1"
                     /translation="MLKLLLIAFWLVVHPVHVTLLSIEYSEEKKTFNAFLRVFYDDFM
                     LDYQLLKGNAFVFDINGDKKEAITRLEGYLKERIQLLAGKMPLDFTITDINLSDNELN
                     IYLQYKSAGKAKVFTVKNAILADIYNDQSNLLIFRYGEFEEGVKLTPGKREHVFEIKR
                     VSD"
BASE COUNT          529 a          378 c          349 g          575 t
ORIGIN      
        1 tgcaattacg tttgttccgt agttatccca ttcaagccgt ataaactttc cttctggtga
       61 agcgctcagg aggtgaggcg cagggccaag cacctttatc ctgaaattgt ctatgtcgaa
      121 caatgaaaga tccctgctgt tatcttctga ttttattatt atatcatagg gttggtgacg
      181 tacattttcg tagcatgttt tccagaaaaa cctggcagat gcatatcctg ccagtgaatc
      241 aattttgctg aaagtggcac tgcacccggg taggctgaaa actccggatg ttgctttaag
      301 tcttatgaaa tcatcatcct tatcggttgc agtaactata aaatccactg tatctcctgc
      361 ttcaacgcag aaatctctga gagtactgtt caccggaggg tggtttttgg tattgtagac
      421 ttcaatctgc atatctctta caaccactcc tattttttta ccattccgcc attcctgaat
      481 ttccattgcc acattatatt ttccagtatc ggcgggggta tcccagacga ggtcgcctga
      541 aattgagtca acataaaatt tatgtgttgc cggcgggaag gtgtagtttt cgataggttt
      601 tccgttttct cttgtgcata cagtaagttg atacgatagg ctgtcaccgt cggggtcgaa
      661 tgcagccgga ttgtgaataa aaagatatcc ttttgcagcc ttgtcgtatg gaggattaag
      721 aagtactggt gtactgttct ttcccattgc ggggttcaca acaagttttg tccttattga
      781 aaatacaaca tttaccgagt ttggtatatt ctggactcca taattgcggt tggggtcctg
      841 tacaaccaca tcgtatactc caggacccgg aaaggtatgg ataattttgt agacattttt
      901 cctgtaataa tttggaagca tcactatttc aatcctggga gcaattgaag aagtgccatc
      961 gccccattca ataggaagct gtggcctgtc ggcataacta agtgtatagg tgaatgtcgt
     1021 aatagtgaat tcataagtaa ggtctgacag ctggacataa gtaatctccc ctgccctgtt
     1081 atgggttgca ttcaaagtga gaatgaatga acccatcaat gccacaacca gcagtaattt
     1141 tttcatactc aattaatcag aaactcgttt aatttcaaac acatgctcac gtttcccggg
     1201 tgtaagtttt actccctctt caaattctcc ataccggaaa atcaaaagat ttgactgatc
     1261 attataaata tcagcaagga tagcattttt tactgtaaaa acctttgctt tcccggcaga
     1321 tttatattgc aagtaaatat tcaactcatt atcagaaaga ttaatatcag ttatcgtgaa
     1381 atccagaggc attttacctg ccagcaattg tattctttct ttaagataac cttccagtct
     1441 ggttattgct tcttttttgt cgccatttat gtcaaataca aaagcattac ctttaagcaa
     1501 ctggtaatca agcatgaaat catcataaaa tactcttaga aaagcattaa atgtcttctt
     1561 ctcctccgaa tattcgatac tcaaaagggt aacgtggaca ggatgaacca ccaaccagaa
     1621 agctattaat aaaagtttaa gcatttattg ctcacaggta attaaaatac tgcaaaaatc
     1681 acgccataaa aacagatttc agacataggt aactctgttt ttcaggtagt ctaccgtacc
     1741 cacttctatg aaggagaatc ctcttccgcc cgagaaatta ccggcaagga taatcacgat
     1801 atcattatcg gacagatcat gtgttttaac c
//
LOCUS       DAOSNB010000336         1808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_65155_length_1808_cov_4.606389, whole genome shotgun sequence.
ACCESSION   DAOSNB010000336 DAOSNB010000000
VERSION     DAOSNB010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65155_length_1808_cov_4.606389"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..980)
                     /locus_tag="PLG42_10715"
     CDS             complement(12..980)
                     /locus_tag="PLG42_10715"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOU31722.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYSAAIAEMINTGVVALTIGQV
                     EDEELKKLGNYGAKKVICVTGDSLKYLDNKIYTSIIAGIAEKENSKVIVFSNNNSGKA
                     IAPGVAVRLKAGVAAAVNALPSSVEPFTIRKKVYSGNAFAHTVIKSEIKVLSLLQNSY
                     DIKETANNAEIERVAWNVEPGMVKTEVLDVQKQTGKVLLTDAEIVVSGGRGMKSPDNW
                     GPLVELAELLGGATACSRPVSDEGWRPHEEHTGQTGKIIAPNLYIAVGISGATQHLAG
                     ISSSKYIVAINIDKDAPIFEAAQYGIVGDAMKVLPKLVEAVRELKK"
     gene            complement(1007..1756)
                     /locus_tag="PLG42_10720"
     CDS             complement(1007..1756)
                     /locus_tag="PLG42_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOU31723.1"
                     /translation="MKILVCISNVPDTTTKIKLSADGKTIDTAGIQWVINPWDELSLT
                     RALELKDDPAAGVTLVTVAHVGTSSSEPTIRKALAIGADDAIRVNAEPADAYFVAAQI
                     AAVLEKEKYDIILCGIESSDYNGSLTGGIIAEFLGIPSVSSVSSLRIENGKPVIVREI
                     DGGKETLTLPVPFVAVVQKGIAKEPRIASMRGIMTARTKQIRVYEPVAVNPLTEIVMY
                     EKPAPRAACKFISPDNPAELIHLLQNEAKVI"
BASE COUNT          452 a          406 c          352 g          598 t
ORIGIN      
        1 aatttacgga attatttttt caattccctt acggcttcaa caagttttgg gagaaccttc
       61 atcgcatcac caactattcc gtattgtgct gcctcaaaaa ttggagcatc tttgtcgatg
      121 tttattgcca caatatattt cgacgaactt atacctgcaa ggtgttgtgt tgctccggat
      181 attcccacgg cgatgtagag gttcggggct attattttac cagtctgtcc ggtatgttct
      241 tcatggggtc tccagccttc gtctgaaaca ggtctcgaac atgcggttgc tccgccgaga
      301 agttctgcca gttctaccag cggtccccag ttatcgggtg atttcatccc cctgccacct
      361 gaaacaacta tttcagcatc ggtaagcaat accttgcctg tctgtttctg tacatccagc
      421 acttctgtct ttaccatacc cggttcaaca ttccatgcca ctctttcaat ttcggcattg
      481 tttgctgttt cttttatgtc gtatgaattc tgcagaaggg aaaggacttt tatttctgat
      541 tttattacag tatgggcgaa ggcattaccc gaatagactt ttttgcgaat agtgaaaggt
      601 tccacgctgg aaggaagagc gtttacagct gctgccactc ccgcctttag tctaaccgcc
      661 actcccggag caatagcttt tcctgaatta ttgttggaaa atacaataac ttttgagttt
      721 tctttttctg caatcccggc aataatggat gtatatatct tattatcaag atattttaat
      781 gaatctccgg taacacatat cactttcttt gctccgtagt tgccaagctt cttaagttct
      841 tcatcttcaa cctgccctat tgtaagggca actactccag tattaatcat ttctgcaatt
      901 gcagcagaat atgaaacgag ttcgaatgaa agtttcttaa actttccgtc ccagttttct
      961 gtataaacta aaactgacat gattttcttt ttgattgatt tgacaattaa ataacttttg
     1021 cctcattctg gagcaggtgt atcagttcgg caggattatc cggactgatg aatttacatg
     1081 ctgctctcgg tgcgggtttt tcgtacataa cgatctcagt gagtggattt acggccacag
     1141 gttcatatac tcttatctgt tttgtacggg cagtcatgat tccccgcatt gatgcaattc
     1201 gtggttcttt tgcaatacct ttctgaacaa ctgcaacaaa tggcacaggc agggtaaggg
     1261 tttccttgcc cccatctatc tctcttacta ttactggttt gccattttca attctgagag
     1321 atgaaacaga tgaaacagaa ggtatgccaa gaaattcggc aattataccg cctgtgagtg
     1381 agccattata atcactcgac tcaatgccgc acaaaataat atcatacttt tccttttcga
     1441 gaactgctgc aatctgtgca gcaacaaaat atgcatcggc aggttctgca ttgactctaa
     1501 tggcatcatc agctcctata gccaatgctt tgcgtatggt aggctctgaa gatgaggtac
     1561 ccacatgtgc cacggtgacc agagtaactc ctgcagcagg atcatctttt aattcaagtg
     1621 cccttgtaag cgacagctca tcccatggat ttatcaccca ttgaatacct gcagtgtcaa
     1681 tggttttacc gtcggccgac aacttaattt tagtggttgt gtcgggtaca ttgctgatac
     1741 aaaccagaat tttcattatg agattttttt gctattgttt tttctttgag gcggcaaata
     1801 taataatt
//
LOCUS       DAOSNB010000337         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2382
            NODE_68081_length_1752_cov_4.106070, whole genome shotgun sequence.
ACCESSION   DAOSNB010000337 DAOSNB010000000
VERSION     DAOSNB010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864449
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:45:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,128
            Genes (coding)                    :: 2,107
            CDSs (with protein)               :: 2,107
            Genes (RNA)                       :: 19
            tRNAs                             :: 16
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 3 of 21
            Pseudo Genes (incomplete)         :: 13 of 21
            Pseudo Genes (internal stop)      :: 6 of 21
            Pseudo Genes (multiple problems)  :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68081_length_1752_cov_4.106070"
                     /isolate="AMR_MDS_2382"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="pyrH"
                     /locus_tag="PLG42_10725"
     CDS             <1..348
                     /gene="pyrH"
                     /locus_tag="PLG42_10725"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122857.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOU31724.1"
                     /translation="KTICLLAAGTGNPFFTTDTAAALRAVEIPADVLLKGTRVDGVYT
                     SDPEKDPSAKKFETISFDEAIQKNLKVMDSTAFTMCRDNNMPIVVFNMNIRGNLFKIV
                     NGEKIGTLVHMAI"
     gene            412..975
                     /gene="frr"
                     /locus_tag="PLG42_10730"
     CDS             412..975
                     /gene="frr"
                     /locus_tag="PLG42_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOU31725.1"
                     /translation="MNEEIELVMEEARERMQKALEHLEHELARMRAGRANPALLDGIT
                     VEYYGVNSPLSQVSNIHTPDPRTILIQPWEKSMLTTIEKAILAANIGLVPVNNGEVIR
                     ITIPPLTEERRLQLVKQVKNEGETARISIRNSRKWANDELKRLRKEGLPEDLEKKAIE
                     DVQEMTDDFIAKVEKVLAIKEKEIMTV"
     gene            complement(1016..>1752)
                     /locus_tag="PLG42_10735"
     CDS             complement(1016..>1752)
                     /locus_tag="PLG42_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOU31726.1"
                     /translation="EILKVMALRMDSLTETERKAFASLQKWDYDMNKDLVAPSVFEFF
                     RFNFPENILADELGELYETMGGTCRDYYLYRIMTEGPDEWIDNIKTDKKETLDDIIFK
                     SFRDCVAEISSKYGTDTAKWKWGDIHRIKLSHPLGSVKLLDFLFGLNSKEYRVGRSNH
                     TISPYTYRKGFEVYHGASERHIFNTSNWDESFTVVPTGVSGIPASEFYLSQTDDYINC
                     RFYMDHFSEPSVKASAKYKAIFKPAR"
BASE COUNT          565 a          388 c          326 g          473 t
ORIGIN      
        1 aagacaatat gcctgcttgc agcaggaacg ggcaacccct ttttcactac cgacaccgca
       61 gcagcactta gagctgtgga aatccctgcc gatgtattgc tgaaaggcac aagagttgac
      121 ggagtatata catccgaccc ggaaaaagac ccttcagcaa aaaaatttga aacaatatca
      181 ttcgacgagg caatccagaa aaacctcaaa gtaatggact caacggcgtt taccatgtgt
      241 cgtgataata acatgcctat tgttgtcttt aatatgaata tcaggggaaa tctttttaaa
      301 atagtgaacg gggagaaaat tggcaccctt gtacatatgg caatttaata ctcaatatct
      361 ataatttatt atttttgttt cgctttaatg gataaataaa catttcagga tatgaatgaa
      421 gaaatagaac tggtaatgga agaagccagg gaaagaatgc agaaagccct tgagcatctt
      481 gaacatgagc tcgcaagaat gcgcgctggc agagctaatc cggctcttct cgacggaata
      541 actgtagaat attacggcgt caactcaccc ctatcccagg tctcaaacat ccatacaccc
      601 gaccccagaa ctatcctaat ccagccatgg gaaaagagca tgcttacaac cattgaaaaa
      661 gctattcttg cagctaatat cggtctggtt cctgtaaata acggcgaagt tattagaatt
      721 actattccac cacttactga agaacggcgg cttcagctgg taaaacaggt gaaaaacgaa
      781 ggggaaacag caaggataag cattcgtaac tcccgtaaat gggctaacga tgaattgaag
      841 aggctccgta aagaaggact gcctgaagac ctcgagaaaa aagcaatcga agacgttcag
      901 gagatgacgg atgactttat cgctaaagtc gaaaaagtac ttgctattaa agaaaaagaa
      961 ataatgaccg tttaagggtt gcaactcttc cccggctctg ttttgcttta cataactacc
     1021 ttgccggctt aaaaatagct ttatatttcg ctgatgcttt tactgaaggc tcactgaaat
     1081 gatccatgta aaacctgcag tttatataat catcggtctg tgacaaataa aattcactgg
     1141 caggaatacc cgaaactcct gttgggacaa ctgtaaacga ctcatcccag ttcgatgtat
     1201 tgaaaatatg cctctcagag gccccatgat atacttcaaa tcctttccga tatgtatagg
     1261 gtgaaatagt atgattgctc cgaccaaccc tgtattcctt tgaattcaac ccgaaaagaa
     1321 agtcaagaag cttaaccgag ccaagaggat gcgataattt tatcctgtga atatctcccc
     1381 acttccactt tgctgtatct gtaccatatt ttgaagaaat ctcagcaaca caatccctga
     1441 aacttttaaa tattatgtca tccagagttt ccttcttatc cgttttaata ttatcgatcc
     1501 attcatcagg tccttctgtc attatccggt ataaataata atccctgcaa gttcctccca
     1561 tagtttcata caattcaccc agttcatctg caagaatgtt ttccggaaag ttaaatctga
     1621 agaattcgaa aaccgatgga gccaccaggt ctttattcat atcataatcc cacttctgca
     1681 gtgatgcaaa tgccttcctc tcagtctcag tcagtgaatc cattcttaat gccattacct
     1741 tcaatatctc cg
//
